LOCUS       DASVQZ010000001         7449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_481177, whole genome shotgun sequence.
ACCESSION   DASVQZ010000001 DASVQZ010000000
VERSION     DASVQZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481177"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1498
                     /gene="folP"
                     /locus_tag="VF547_00005"
     CDS             413..1498
                     /gene="folP"
                     /locus_tag="VF547_00005"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8261236.1"
                     /translation="MTQRHYLRPTAFVDAPFGYDGKVARLAGGMLWFAAVELVAMEGD
                     RRLSSELVPVERIEEALAALGAPAQRAWRHLTSPRAPLTLRERVVRLDQPQVAGILNV
                     TPDSFSDGGRFESGEAAAEAGFAMAEAGAAIVDVGGESTRPGAKPVWEGDEIERVLPA
                     VTRLAAGGTAVSVDTRKAAVMEAALAAGAAMVNDVSALTYDDRSPEVAAAAGCPVVLM
                     HHLGSPETMQHSPSYDGPVLFAVYDWLEERIEAAEAAGIDRARIIVDPGIGFGKTVQH
                     NLDLLNGLALLHGLGCPVMLGASRKRMIGALSAEAPAGERLPGSLALALKGVDQGVQL
                     LRVHDVPETVQALRVWRGLRDAALTPA"
     gene            complement(1623..2201)
                     /locus_tag="VF547_00010"
     CDS             complement(1623..2201)
                     /locus_tag="VF547_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX8261237.1"
                     /translation="MTAGSGAPDPDGADEPPRPEDEALIKLTGTMKWFDATRGFGFLV
                     SEQAKGDILVHFSVLKEHDRRSLPEGAIVECLVAEQERGLQARRVLSIDLSHAVVSEP
                     QRPSGGGGERVDPSALLDEAGEFEPVRVKWFNRLKGYGFLVRDDEDGHDIFVHMETVR
                     RGGLTDLTPDQPLRARIAEGRKGPLAVEVEPR"
     gene            complement(2295..2831)
                     /locus_tag="VF547_00015"
     CDS             complement(2295..2831)
                     /locus_tag="VF547_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX8261238.1"
                     /translation="MRSRPDKRPRPPLDSEALERLGLFYAGRYSTTRARLAAYLRRKL
                     RERGWSGPGEAPVERLVERFAELGYVDDRAFASARAESLLRRGYGERRVRDALKGAGI
                     AEEDSADAQGRAGEEALAAAHRFARRRRLGPYAASAPDRDQLRRAAAAMIRAGHRSDL
                     VREVLGSGPGDLPDPDVP"
     gene            2938..4674
                     /locus_tag="VF547_00020"
     CDS             2938..4674
                     /locus_tag="VF547_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8261239.1"
                     /translation="MPETLVPTPTADRLPRRFADFETLGEALDYAARGERGLNFHDAR
                     GALVRAYPYRELRADALAAAFRLIAAGVAPKDRVAIVAETSPEFAALFFGAVYAGAWP
                     VPLPLPTSFGGRDSYIDQLKVQLDSADPARLFYPAELAALAGAAAEAAGVPGESWESF
                     ERREGPEAELPGAGPDDIAYLQYSSGSTRFPHGVAVTHRGLLNNLAAHSHGMEVIETD
                     RCISWLPWYHDMGLVGCLLSPMANQISTDYLKTEDFARRPLAWLDLITRNEGTTGSYS
                     PTFGYDICARRVSSLTNVAERFDLSRWRIAGNGADMIRPDVMQAFVDAFAPAGFSAKS
                     FCPSYGLAEATLAVTIMPPGEGIVVELVEETTLSGDGKMHRDRPQRYRAIVNCGKPAR
                     DMELEIRDEDGRTLGNREIGKVHCRGPSVMVGYFRDEEATAACLDPDGWLDTGDMGYL
                     SDGYLYIVGRAKDMIIINGKNHWPQDIEWAVEQLPGFKAGDIAAFSITTPGGEETPAV
                     LVQCRTSDAAERSRLREIIRERVRAITGMNCVIELVPPRTLPRTSSGKLSRAKARSLY
                     LTGEIQPYDIAA"
     gene            4857..6944
                     /locus_tag="VF547_00025"
     CDS             4857..6944
                     /locus_tag="VF547_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX8261240.1"
                     /translation="MINFVDAGGQPNNPSAAAANLALIHTLIANTSVANPGGHDYWGA
                     GPHIYFPPGNYHFSGPIRIRKAVRLEGSSAATRANYNTIFSFPVNSQGLVLDSFDTDN
                     LSYLSYANRQTTGAGTRIEGIYFRGSNNALNNAHGVLIRAFVMMRDVHVTGFGGHGIY
                     IKADLSENNGGQASNSQLYDCGAVGNNRSGLVIEGGDANIVQVIGGDYSFNGDWGVAD
                     LSFLGCTFSGIHATQNGQPNAGHRGTTEVSLVTYPGGSGQVWAIRANSAASGLSTEPG
                     TNEMVWTRYHGQLSWTPPTWTVNNSGNLIIRPGGPYLTASIVAWSVWTGCYSEGNQPP
                     SQMSHRAIVIGGDHGSSIQAVDNSGTGQPIWLDAHNAELDLKTNLRVKGLLLGQKRAR
                     PNDSVGMKMSAASAAPVSGTLYNVAPFMDYSWSPGDIVWNQDRSATQSGVTPPLGWRC
                     TAGPDSFEEIPFPTSSSIGANPTFTTVTANQFIVPSQGSISGTGGELYVRGGWVRITN
                     AAATQTYGLFTGSTLQLPGLATTASGANAFLDAGLSNALYRSTSSLRYKTDVKDVDGK
                     YLDKLLDLRPVRYRSTAEADDPSLSHFGLIAEEVAEIEPRLVNFTYADEDYEEVAVTA
                     EDGEGGPRTERRLREGAERKVPDGVQYDRLTVLLLGLAKRQQGRIEALEGRLADLAER
                     LPATDAEARPKRS"
     gene            7108..7185
                     /locus_tag="VF547_00030"
     tRNA            7108..7185
                     /locus_tag="VF547_00030"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7143..7145,aa:Trp,seq:cca)
BASE COUNT         1165 a         2702 c         2475 g         1107 t
ORIGIN      
        1 atgttctggc atttcgagga ggccgggagc ctgctgccgc tcgacgcgct tcgccagcgc
       61 cacctgctga tgctggactg actcggaccg aagcaaaaaa ggtttcaccg tgccactcga
      121 cacgagcgtg aaacgaaggc acattggcgg gcggcgaccg tcccttcacg cccaacgccc
      181 gcacgccttt gaccacggga gacgcgcggg agcccctttg ggcgctgatg ggcccggacc
      241 gataccatgc cccacatggt ggcccgggcc tcggagaatt cagttaccct gctgaccttc
      301 gaaaagaggc ggtcgtcacc caagacctcg atcgcccgaa tcggccgatc acggccttag
      361 tctccgagct tcgggattcc tgtcgctaca attttgcgct agccgcattg ccatgaccca
      421 gcgccactat ctgcggccca ccgcgttcgt cgacgcgccc ttcggctacg acggcaaggt
      481 ggcgcggctg gccggcggca tgctgtggtt cgcggccgtc gagctcgtcg cgatggaggg
      541 cgaccgaagg ctgtcctcgg agctggttcc ggtcgagcgg atcgaggagg cgctcgccgc
      601 gctcggcgct ccggcgcagc gcgcctggcg ccacctgacc tcgccgcgcg cgccgctgac
      661 cctgcgcgag cgggtcgtcc ggctcgacca gccgcaggtc gcggggatcc tcaacgtcac
      721 tccggacagc ttctccgacg gcggccgctt cgaaagcggg gaggcggccg cagaggcggg
      781 cttcgccatg gccgaggcgg gcgccgcgat cgtcgacgtc ggcggcgaat cgacccggcc
      841 cggcgcgaag ccggtctggg agggcgacga gatcgaacgg gtgctgcccg ccgtcacccg
      901 gctcgccgcg ggcgggacgg cggtgtcggt cgacacgcgc aaggcggcgg tgatggaggc
      961 ggcgctcgcc gccggcgccg ccatggtcaa cgacgtctcc gccctcacct atgacgaccg
     1021 ttcgccggag gtcgccgccg ccgccggctg tccggtcgtg ctgatgcacc atctcggcag
     1081 ccccgagacg atgcagcact cgccctccta cgacgggccg gtgctgttcg ccgtctacga
     1141 ctggctcgag gaacggatcg aggcggccga ggcggcgggg atcgaccgcg cccggatcat
     1201 cgtcgacccc ggcatcggct tcggcaagac cgtccagcac aatctcgatc tgctcaacgg
     1261 cctcgccctc ctccacggcc tcggctgccc ggtcatgctc ggcgccagcc gcaagcggat
     1321 gatcggcgcc ctgtcggcag aggcgcccgc cggcgagcgg ctgcccggct cgctcgccct
     1381 cgccctcaag ggcgtcgatc agggggtgca gctcctccgg gtccacgacg tgcccgagac
     1441 ggttcaggcg ctccgggtgt ggcgaggcct gcgcgacgcg gcgctgacgc cggcgtaaac
     1501 cctctccctc aaggggagag ggaggcgccc aaagcgaagc tttgggaggg tgagggcacg
     1561 gcggaggcag gccctcaccc tgccgcttcg cggctgtccc tctcccctgc agggagaggg
     1621 tttcagcgcg gctcgacctc gaccgcgagg gggcccttgc ggccttcggc gattcgggcg
     1681 cgcagcggct ggtccggcgt gaggtcggtg aggccgccgc ggcggaccgt ctccatgtgg
     1741 acgaagatgt cgtggccgtc ctcgtcgtcg cggaccagga agccgtagcc cttgaggcgg
     1801 ttgaaccatt tcacccgaac cggctcgaac tcgccggcct cgtcgagcag ggcggacgga
     1861 tcgacccgct cgccgccccc gccggacggg cgctgcggct cggagacgac ggcgtggctg
     1921 agatcgatcg acaggacgcg ccgcgcctgc aggccgcgct cctgctccgc caccaggcat
     1981 tcgacgatgg cgccctcggg caggctccgg cggtcgtgct ccttgagcac gctgaaatgc
     2041 acgaggatgt cgcctttcgc ctgctcgctg accaggaagc cgaagccgcg ggtcgcgtcg
     2101 aaccatttca tcgtgccggt gagcttgatc agcgcctcgt cttcagggcg cgggggttcg
     2161 tccgcgccgt cggggtcggg cgcgccgctt ccagcagtca ttccgcggcg cgaagtcatt
     2221 aacggccgat ccacgatgac gaaccgggtg agtatcacgg cttttgcgga tttgggaacc
     2281 gtttacggcc gtttttatgg gacatccggg tcaggcaggt cgcccggccc gcttccaagc
     2341 acctcgcgga ccagatcgga ccggtggccg gcccgaatca tcgccgcggc cgcgcggcgc
     2401 agctggtcgc ggtccggggc gcttgcggca taggggccga gccggcgccg gcgggcgaaa
     2461 cgatgggcgg cagccagcgc ctcctccccg gcccggccct gcgcgtcggc ggagtcctcc
     2521 tcggcgatgc cggcgccctt cagagcgtcg cgcacccgcc tttcgccata gccgcgccgg
     2581 agcagggact ccgcgcgcgc cgaggcgaag gcgcggtcgt cgacatagcc gagctcggcg
     2641 aagcgctcga cgagccgctc caccggcgcc tcgcccgggc cgctccagcc gcgctcccgg
     2701 agcttccggc gcagataggc ggcgagcctg gcgcgggtgg tcgagtagcg gccggcatag
     2761 aagaggccga gccgctcgag cgcttccgaa tcgagcggcg gccggggcct tttgtcgggg
     2821 cgggatctca cgcgccacga ttgtgccaca gttcggcccg atgataaacg cgaacctctc
     2881 ccgtccgcgg gcggggttcg tccataattg gccacgacga gaggatccgc caccgcagtg
     2941 cccgagactt tggtcccgac gccgaccgcg gataggttgc cgcgccgctt tgccgatttc
     3001 gagacgctcg gggaagcgct cgactatgcg gcgcgtggcg aacgcggcct caacttccac
     3061 gatgcgcgcg gggcgctggt gcgcgcctat ccgtatcgcg agctgcgcgc cgacgcgctt
     3121 gccgcggcct tccggctgat cgcggcgggg gtggcgccga aggaccgggt ggccatcgtc
     3181 gccgagacca gcccggaatt cgccgccctc ttcttcggcg ccgtctatgc cggcgcctgg
     3241 ccggtgcctc tgccgctgcc gacctcgttc ggcggccgcg attcctacat cgaccagctc
     3301 aaggtccagc tcgacagcgc cgatccggcc cggctcttct accccgccga gctggccgcc
     3361 ctcgccggag cggcggcgga ggcggccgga gtcccgggcg aatcctggga gagcttcgag
     3421 cgccgcgagg ggcccgaggc cgagcttccc ggcgccggcc ccgacgacat cgcctatctc
     3481 caatattcga gcggctcgac ccgcttcccc cacggagtcg ccgtcaccca ccgcggcctc
     3541 ctcaacaacc tcgccgcgca cagccacggc atggaggtga tcgagaccga ccgctgcatc
     3601 tcctggctgc cctggtatca cgacatgggc ctggtcggct gcctgctgtc gccgatggcc
     3661 aaccagatct cgaccgacta tctcaagacc gaggatttcg cccgccgccc gctcgcctgg
     3721 ctcgacctca tcacccgcaa cgagggcacg accggaagct attcgccgac cttcggctac
     3781 gacatctgcg cccgccgcgt ctccagcctg accaacgtcg ccgagcgctt cgacctgtcg
     3841 cgctggcgga tcgccggcaa cggcgccgac atgatccggc ccgacgtcat gcaggccttc
     3901 gtcgacgcct tcgcccccgc cggcttttcg gcaaagagct tctgccccag ctatggcctc
     3961 gccgaggcga ctctggcggt gacgatcatg ccgccgggcg agggcatcgt cgtcgagctg
     4021 gtcgaggaga ccaccctgtc cggcgacggc aagatgcacc gcgaccggcc gcagcgctac
     4081 cgggcgatcg tcaattgcgg caagcccgcg cgcgacatgg agctggagat acgcgacgag
     4141 gacggccgga ctctcggcaa ccgcgagatc ggcaaggtcc attgccgagg gccctcggtg
     4201 atggtcggct atttccgcga cgaggaggcg accgcggcct gcctggaccc ggacggctgg
     4261 ctcgacaccg gcgacatggg ctatctcagc gacggctatc tctacatcgt cggccgcgcc
     4321 aaggacatga tcatcatcaa cggcaagaac cactggccgc aggacatcga atgggcggtg
     4381 gagcagctgc ccggcttcaa ggcgggcgac atcgccgcct tctcgatcac cacgccgggc
     4441 ggcgaggaga ccccggcggt cctcgtccag tgccgtacct ccgacgccgc cgagcggagc
     4501 cggctccgcg agatcatccg cgagcgggtg cgcgcgatca ccgggatgaa ctgcgtcatc
     4561 gagctggtgc cgccgcgcac cctgccgcgg acgtcgtccg gcaagctcag ccgcgccaag
     4621 gcgcgcagcc tctacctcac cggcgagatc cagccctacg acatcgcggc ctagagcccg
     4681 ccgccttcag ccccgcctga atccctcctc ccgtcgacag cgggaaagag gaacgagtcg
     4741 cgtcctgcgg aaactcccta ttgaattcct attaacctgt tctatatatg ttcctctcgc
     4801 gaacgtcggc tgtggggaag tgccgccgtt cgccctcacg tggcaaagga acagacatga
     4861 tcaatttcgt cgacgcgggc ggccagccca acaatccgag cgcggccgcc gccaacctgg
     4921 ccctgatcca tacgctgatc gccaacacga gcgtcgccaa tccgggcggc cacgattatt
     4981 ggggcgccgg cccgcacatc tactttccgc ccggcaacta tcatttcagc ggcccgatcc
     5041 ggatccgcaa ggcggtccgc ctggagggct ccagcgccgc cacccgggcc aattacaaca
     5101 cgatcttcag ctttccggtc aactcgcagg gcctcgtcct cgacagcttc gatacggaca
     5161 acctgtccta tctcagctac gcaaatcgcc agaccaccgg cgccggcacc cgaatcgagg
     5221 gcatctactt caggggctcg aacaacgccc tcaacaatgc ccatggcgtg ctgatccggg
     5281 cgttcgtgat gatgcgggac gtgcacgtca ccggcttcgg cgggcacggc atctacatca
     5341 aggccgatct gtccgagaac aacggcggcc aggcgtcgaa cagccagctc tacgattgcg
     5401 gcgccgtcgg caacaaccgc tccggcctgg tgatcgaggg cggcgacgcc aacatcgtcc
     5461 aggtcatcgg cggcgattat tcgttcaacg gcgactgggg agtcgccgac ctgagcttcc
     5521 tcggctgcac cttctccggc atccacgcca cccagaacgg gcagcccaat gcgggccacc
     5581 gcggcacgac cgaggtcagc ctcgtcacct atccgggggg cagcggccag gtctgggcca
     5641 tccgtgccaa cagcgcggca agcgggctca gcaccgagcc gggcaccaac gagatggtct
     5701 ggacccgcta tcacgggcag ctgagctgga ccccgcccac ctggaccgtc aacaacagcg
     5761 gcaatctcat cattcgtccc ggcggcccct atctcaccgc cagcatcgtc gcctggtcgg
     5821 tctggacggg ctgctattcc gagggcaacc agcccccgtc gcagatgagc caccgcgcga
     5881 tcgtcatcgg cggggaccat ggctccagca tccaggcggt cgacaatagc ggcacgggcc
     5941 agccgatctg gctcgacgcc cacaatgccg agctcgacct caagaccaac ctcagggtga
     6001 agggcctcct gctcggacag aagcgagcga ggcccaacga ttcggtgggg atgaagatgt
     6061 ccgccgcttc ggccgccccg gtctcgggga ccctctacaa cgtcgcgccc ttcatggact
     6121 acagctggtc ccccggcgac atcgtctgga accaggaccg gtccgcgacc cagtcgggag
     6181 tcactccgcc gctcggctgg cgctgcacgg cggggccgga cagcttcgag gagataccct
     6241 tcccgacctc gagcagcatc ggcgccaacc cgaccttcac gacggtcacg gcgaaccagt
     6301 tcatcgtgcc gtcccagggc agcatctcgg gcacgggcgg cgagctctac gtccgcggcg
     6361 gctgggtgag gatcaccaac gccgcggcga cccagaccta cggcctgttc acgggaagca
     6421 ccctccagct gccgggactg gccaccaccg cctcgggcgc caacgccttt ctcgacgccg
     6481 ggctgagcaa cgccctgtac cgctcgacct cgtcgctgcg ctacaagacc gacgtcaagg
     6541 atgtcgacgg caagtatctc gacaagctgc tcgatcttcg accggtccgc taccgttcga
     6601 cggccgaggc cgacgatccg tcgctcagcc atttcggcct gatcgccgag gaggtcgccg
     6661 agatcgagcc gcgcctcgtc aacttcacct atgccgacga ggattatgag gaggtggccg
     6721 tgacggcgga ggacggcgag ggcggccccc ggaccgagcg caggctcagg gagggcgccg
     6781 agcgcaaggt gcccgacggg gtccagtacg accggctgac cgtcctcctg ctcggcctgg
     6841 ccaagcgcca gcagggcagg atcgaggccc tcgagggccg gcttgccgac ctcgccgagc
     6901 ggctgcccgc gacggatgcc gaggctcggc cgaagcggtc ctgaccggac ggccggcgtc
     6961 cttcttccca gcgccggccg cagccccccc gctcttcccg tggggcgggg ggctacacgc
     7021 cgtcggaggg cgggcggcga tgcctccggc gggagaaggc accttgcgcg gatcggccgc
     7081 cgccgttagt tcgttcgttc cggctctagg agtgtagctc agcctggtta gagcatcggt
     7141 ctccaaaacc gagggtcgtg ggttcgaatc cctccactcc tgccagccgg agcgaggcgc
     7201 gaagcggcgc aggtgcccgc ccggcgccgc cggccgccgc ttgaaccgcg atcgacgccg
     7261 cgcgattcac tcgagcgacg ccatggcgtc gtaaagctgc cggggcttcg tccccgccgg
     7321 ccttgcgttc cgccctcccc gacgctatct cgcgaagctc gcatgcatcg gcaaagctcg
     7381 gctgaccggc cccccggtcg agagccgctg cccgaaaggc atttcagaag gacgaagaga
     7441 cgagcatgg
//
LOCUS       DASVQZ010000002         5037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_673684, whole genome shotgun sequence.
ACCESSION   DASVQZ010000002 DASVQZ010000000
VERSION     DASVQZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673684"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1058)
                     /locus_tag="VF547_00035"
     CDS             complement(45..1058)
                     /locus_tag="VF547_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8261241.1"
                     /translation="MSGGWEVAATGAAEGIALVVIITGLLQTLFYVVQLAFAGFALGR
                     RPPVARASSLWNRYSDEAPAISVLAPAFNEEVTIVQSVKSLLALHYPDFEVIVVNDGS
                     KDGTLDRLIEGFGLGPVERFHELAVEHRPIRGLYASERLPRLLVVDKENGGKSDALNA
                     GINLARSPIFCSMDADSLLEPDALIRAVRPFVEDPTRTVAVGGTIRIANGSRIEGGRV
                     LDVGLPRNLLALIQIVEYLRAFLMARLALSKMGVLTVISGAFGLFRRQTAVAVGGYRH
                     GTVGEDMELVVRLHRRLREQKIAYRIEFIPEPVCWTEVPEDLTILGRQRARWQRGALE
                     TFA"
     gene            complement(1055..2092)
                     /locus_tag="VF547_00040"
     CDS             complement(1055..2092)
                     /locus_tag="VF547_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8261242.1"
                     /translation="MSGLLMLWFTSLAMTGAALAIMVGLLLARWGTRWRARARAEERR
                     RLVPLLLGDGEEEIRPLLWLADKFIAELMVELMQLVRGRERERFTARAEALGVPERLR
                     GQLGSGSSRVRQTAAEALAYFEDERTTEGLRHALGDSNPDVRLTAALALAQSGRAPPL
                     RELVYRLDIGRSEKSLLVNALFKELARHGTAELEALLRSPDAPLTARIAAADALAEAG
                     DYGSVSAIAEAALGASENGPELPRMLRALARLGHPAAAPAVARALASAAPSARAAAAE
                     AAGRIGFANMADRLAELLGDPSWRVRFRSAQALVALGDEGLARLRSAAAGGGPPASEA
                     ATRILGEKGLA"
     gene            complement(2089..3213)
                     /locus_tag="VF547_00045"
     CDS             complement(2089..3213)
                     /locus_tag="VF547_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEX8261243.1"
                     /translation="MSAALLAAALAAARPAAPPAGPEARSLYEQGVAARLAGDPERSI
                     LLLRQAAEADPANADLQLQLGLALMAAGRIEEAEAALRETLRLAPAYDDARIALARLA
                     RRRGDDRAARAELDRVSPTNPEAAELRSGLAAPGGGAASAPASAHRWRVDLDGSYSAL
                     GGGRPDWREASLAVSRRLGSSTHVSGQLERSRRFELDDTYGEVRLDRRLEGGGFHLFA
                     GGTPNADFRPRWQIGGGASVRLRSGPSATILTLDARQAHYRSGDIQTVSPGFEQYLAG
                     GRFWLSGRLINIFDESGRHHLGWLARGDFQALASLRFFAGLADSPDTSAGRVVDTFSQ
                     FAGLSWDAGERTTLRLSLAREDRDGGGDRLQIGLGLGLRF"
     gene            complement(3210..3587)
                     /locus_tag="VF547_00050"
     CDS             complement(3210..3587)
                     /locus_tag="VF547_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8261244.1"
                     /translation="MRKKILVCDDDPLLLDLVQFKLTARGYDSSAARDGGEAMAALAE
                     QAPDAVVLDAMMPVLDGYEVLRRIRENPEFAELPVIMLTARRQEQDVLSALQLGANDY
                     MVKPFSPEELVARLGRLLEAAPE"
     gene            complement(3748..>5037)
                     /locus_tag="VF547_00055"
     CDS             complement(3748..>5037)
                     /locus_tag="VF547_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX8261245.1"
                     /translation="QYGCFDQNQNIKIGTDPSGQFITSWVCDQGDVGGVETYVDNRPG
                     PLGPNGFGFGTWGQSNRYTSINGHILPTFYAKQGAVERWRLIHGGVRDTIRLIFLKAL
                     SSPDLKPGNHLKAENMDALIETTCGFSTTPFLLAAADGLTMNQAQTTRSATLQPGYRF
                     DALVQFPTEGDYCVVDALSGAADSVGGVARGARLLGIVRVSPDPAQVNRGTVAEQLID
                     SAQKTMPAAVRGEVISDLQNGLLLRRFAPHQTIAQSELSGGQEMTFDIQGNQPPFMFG
                     VANQLPTSPAYNPQPYNPDRLDRKLILGTAEEWKLTSNAAGHPFHIHVNPFEIVSIIN
                     NNNPSVDLSAPTAPVDFGDPQYRGLRGVWKDTLWIKPGYTATVRTRYQRYIGDFVLHC
                     HILDHEDEGMMQNVAVVLPGGSSASVNAPADGHGNHH"
BASE COUNT          777 a         1721 c         1801 g          738 t
ORIGIN      
        1 cacgccaccc gcccgtagcg cgcgacgttc atcatgtccc ggtgctaggc gaaggtctcg
       61 agcgcgccgc gctgccagcg ggcgcgctgc cggcccagga tggtgaggtc ctcgggaacc
      121 tcggtccagc agaccggctc ggggatgaac tcgatccggt aggcgatctt ctgctcgcga
      181 agccgccggt gaagccgcac gaccagctcc atgtcctctc ccaccgtgcc atgccggtag
      241 ccgccgacgg cgaccgcggt ctgccggcgg aacaggccga acgcgccgga aatgacggtc
      301 agcaccccca tcttgctgag cgccagtcgg gccatcagga aggcgcgcag atattcgacg
      361 atctggatga gcgcgagcag gttcctcggc agcccgacgt cgagcacccg tccgccctcg
      421 atccggctgc cgttggcgat ccggatcgtc cctccgaccg ccacggttcg ggtgggatcc
      481 tcgacgaagg gccggacggc ccggatcagg gcatcgggct cgagcagcga atccgcgtcc
      541 atcgagcaga agatcggcga gcgggcgagg tttatgccgg cgttcagggc gtcggacttg
      601 ccgccattct ccttgtccac gaccagcagc ctcggcagcc gctcgctggc atagagcccc
      661 cggatcgggc ggtgctcgac cgccagctca tggaagcgct cgaccgggcc gaggccgaag
      721 ccctcgatga gccggtccag agtcccgtcc ttcgatccgt cgttgacgac gatcacctcg
      781 aaatccggat agtggagagc gagcagcgac ttgacgcttt ggacgatcgt cacctcttcg
      841 ttgaaggccg gtgcgaggac cgagattgcg ggcgcctcgt cgctgtagcg gttccacaag
      901 gaggaggccc gagcgaccgg cggccgccgg cccagggcga agccggcgaa ggcgagctgg
      961 acgacgtaga agagggtctg gagaaggccc gtgatgatga cgacgagggc gatcccctcg
     1021 gcggctccgg tcgcggcgac ctcccagccg ccgctcacgc caggcccttc tcgccgagaa
     1081 tacgggtggc ggcttccgag gccggaggcc ctccgccggc ggcggcggag cgcagtcggg
     1141 ccaggccctc gtcgcccagg gcgaccagcg cctgggccga acggaagcgc acgcgccagc
     1201 tgggatcgcc gagcagctcg gcgagccggt ccgccatgtt cgcgaacccg atccggcccg
     1261 ccgcctcggc cgccgccgcc cgggcggacg gagcggcgct ggccagcgcc ctggcgaccg
     1321 ctggagcggc ggcgggatgg ccgagccggg caagcgctcg aagcattcgc ggcagctcag
     1381 gaccgttttc ggaagcgccc agggcggcct cggcgatggc cgagaccgac ccgtagtcgc
     1441 cggcttcggc caaggcatcc gccgccgcga tccgggcggt gagcggggcg tccggcgagc
     1501 gaagcagcgc ctcgagctcc gcggttccgt gccgggcgag ctccttgaag agcgcgttga
     1561 cgagcaggga tttctcgctc cggccgatgt cgagcctgta gaccagctcg cgaagcggcg
     1621 gcgcccggcc cgactgggcg agggcaagcg ccgcggtcag ccggacgtcg gggttgctgt
     1681 cgccgagggc gtggcgaagc ccctccgtcg tccgctcgtc ctcgaaataa gcgagcgcct
     1741 cggcggcggt ctggcgcacc cgcgacgaac cgctcccgag ctggccgcga agccgctccg
     1801 gaacgcccag cgcctcggct cgggcggtga accgctcccg ctcccggcct cggacgagct
     1861 gcatcagctc caccatcagc tcggcgatga acttgtcggc aagccataga agcggcctga
     1921 tctcctcctc gccgtcgccg agcaggagcg ggacgagccg cctgcgctcc tcggcccggg
     1981 cgcgggcgcg ccatctggtt ccccagcgcg ccaggagcag cccgaccatg atcgcaagcg
     2041 ccgctccggt catcgccagg gacgtgaacc agagcatgag caatccgctc aaaatctgag
     2101 ccctaggccg agtccgatct gcagccggtc gccgccgccg tcccggtctt cgcgggcgag
     2161 cgacaggcga agggtcgtcc gctcgcctgc atcccaggac agaccggcga actggctgaa
     2221 cgtatccacc acccgcccgg ccgacgtgtc gggggagtcg gcgaggccgg cgaagaaacg
     2281 caggctcgca agcgcctgga aatcgcctcg agcgagccag ccgagatggt ggcgcccgct
     2341 ttcgtcgaag atgttgatca gccgtccgct cagccagaag cggcctccgg cgagatattg
     2401 ctcgaagccg ggggagacgg tctggatgtc gccggagcgg taatgcgcct ggcgggcgtc
     2461 gagggtcagg atggtggccg acggccccga gcgaagccgg acggaggcgc ctccgccgat
     2521 ctgccagcgc gggcggaaat cggcgttcgg cgtgcccccg gcgaaaagat ggaagcctcc
     2581 gccttccagg cgccgatcca gccggacttc gccataggtg tcgtccagtt cgaaccggcg
     2641 cgaccgctcg agctgccccg acacatgggt ggagcttcca agccgccgcg agaccgcgag
     2701 gctggcctct cgccagtccg gccggccgcc gccgagcgcg ctgtagcttc cgtccaggtc
     2761 gacccgccag cgatgggcgg aggcgggcgc cgaggcggcg cctccgccgg gtgcggcgag
     2821 gccgcttcga agctcggccg cctcggggtt ggtgggactg acccgatcga gctctgcgcg
     2881 agccgcccgg tcgtcgcccc ggcggcgggc gagccgggcg agggcgatcc gggcgtcgtc
     2941 ataagccggc gccagccgaa gcgtttccct gagcgccgcc tccgcctctt cgatgcgccc
     3001 ggccgccatc agcgccagcc ccaattgcag ctgcaggtcc gcattggcgg gatcggcctc
     3061 ggcggcttgc ctcagaagca ggatcgagcg ctcgggatcg ccggccaggc gggcggccac
     3121 gccctgttcg tagaggctcc gagcctccgg tccggccgga ggggccgcgg gccgggcggc
     3181 cgccagggcc gccgccagca gtgcggcgct cattccggag cggcttccag aaggcggccg
     3241 agccgggcga ccagctcctc ggggctgaac ggcttcacca tataatcgtt ggcgccgagc
     3301 tggagagcgc tcagcacgtc ctgctcctgt cgccttgccg tcagcatgat caccggaagc
     3361 tcggcgaact ccggattctc gcggatccgc cgaagaacct catagccgtc gagcaccggc
     3421 atcatcgcgt cgaggacgac cgcatcgggc gcctgctcgg cgagagcggc catggcttcg
     3481 ccgccgtcgc gcgcggcgct cgaatcgtag ccgcgcgcgg tcagcttgaa ctgcaccagg
     3541 tcgagaagca gcggatcgtc gtcgcagacc aggattttct tacgcacact caattcccat
     3601 gaccggtcga acgcatctgg cggtggcttg atccgccacc cgccgtccat cccgcctggc
     3661 cccggcgagc gcaagcccgg accgcaacga aaaaggcccc gccttgcggc ggggccttcc
     3721 gtcgttccga gcccggaggc gaaggactta gtggtggttg ccgtgtccgt cggccggcgc
     3781 gttgacgctg gccgaggagc cgccgggcag caccaccgcg acgttctgca tcatgccttc
     3841 gtcctcatgg tcgaggatgt ggcaatggag cacgaagtcg ccgatgtagc gctggtagcg
     3901 ggtgcggacc gtggccgtgt agcccggctt gatccacaac gtgtccttcc acactccgcg
     3961 gagaccgcga tattgcgggt cgccgaagtc gaccggagcg gtcggagcgc tgagatcgac
     4021 gctcggattg ttgttgttga tgatcgagac gatctcgaac gggttgacgt ggatgtggaa
     4081 ggggtggccc gccgcgttgg aggtcagctt ccactcctcg gccgttccga ggatcagctt
     4141 acggtccagc cggtccggat tgtagggctg gggattgtag gccggcgacg tcggcagctg
     4201 gttggcgact ccgaacatga agggcggctg gttgccctgg atgtcgaagg tcatctcctg
     4261 gccgcccgaa agctcgctct gagcgatcgt ctggtgcggg gcgaaacggc ggagcaacag
     4321 gccgttctgg aggtcggaga tgacctcgcc gcggaccgcg gccggcatgg tcttctgggc
     4381 cgagtcgatc agctgctccg cgacggtgcc gcggttcacc tgcgccggat ccggcgaaac
     4441 ccggacgatg cccagcagac gcgcgccgcg cgccactccg ccgacgctgt cggcggcgcc
     4501 gctcagggcg tcgacgacgc aataatcgcc ctccgtcggg aactgaacca gggcgtcgaa
     4561 gcgatagccc ggctgcaggg tggcggagcg ggtggtctgg gcctggttca tggtcaggcc
     4621 gtcggccgcc gcaagcagga agggcgtggt cgagaagccg caggtcgtct cgatgagagc
     4681 gtccatattc tcggccttga ggtggttgcc cggcttgaga tccggcgacg acagcgcctt
     4741 gaggaagatg agtcggatcg tgtcgcggac tccgccgtgg atcaggcgcc agcgctcgac
     4801 cgcgccctgc ttggcataga aggtgggaag gatgtggccg ttgatgctgg tgtagcggtt
     4861 cgactggccc caggtgccga aaccgaagcc gttcggaccc agcggcccgg gccggttgtc
     4921 gacataggtc tcgaccccgc cgacgtcgcc ctggtcgcag acccacgaag tgatgaactg
     4981 gcccgacgga tcggtgccga tcttgatgtt ctggttctgg tcgaagcagc catattg
//
LOCUS       DASVQZ010000003        22168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_252,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000003 DASVQZ010000000
VERSION     DASVQZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 22168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22168
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VF547_00060"
     CDS             complement(<1..1371)
                     /locus_tag="VF547_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX8261246.1"
                     /translation="MPHLYLVDGSSYIFRAYHRLPPLTNRHGLNVGAVYGYTTMLWKL
                     AESLHKEEGPTHLAVIFDAGEKTFRNERYDQYKANRPPPPPDLVPQFPLIRDATRAFS
                     LPCIEEQGLEADDIIACYAKAALEQGWKVTIVSSDKDLMQLVEDGRLDLYDTMNDRRL
                     GRDYVIEKFGVPPEKLGDVLALMGDSVDNVPGVPGIGPKTAAQLIQEYGDLESVLAHA
                     EEIKKPKLRENLLLHTDAARLSRELVTLVCDAPLPEPLGDLEMKGIPDGPLRAFLEEQ
                     GFKALLARLNGTSQVKAPSDSAAPVQREVRPEPKIDRSAYATVTSEAELDRWIAEARA
                     NGLVAVDTETDGYDCVTATLVGISLATECGRACYVPLEHGGHDLLSERPEQIRAEVAL
                     ARLKPLFEDPAVLKIGHNFKFDWIVFDRRGIKVAPVDDSLVMSFNLDAGGLASHAMDD
                     LARKHLD"
     gene            1483..2340
                     /locus_tag="VF547_00065"
     CDS             1483..2340
                     /locus_tag="VF547_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261247.1"
                     /translation="MSRGLLPLSCLALAPFAATTAAAAPFEPTRFSVAVRGTGPDVIL
                     IPGLAASRGIWNSTAAAVPGYRYHLVQVAGFAGTAAAGNRSGPVAAAVADEIARYVVA
                     NRLGRPAIVGHSMGGSIALMIGARHPQSAGRVMVVDMLPSPARPLGIPALVMKPLARL
                     VGGEMQRASRFRLRIKPLVERFGNADWLESRSDGDVIGRSVEELMATDLTPELSRIRA
                     PLTVVYACSPTLGSACGRVSRIYADAYAARPGTRLVRIDRSGHTIMLDQPAAFQGALR
                     AFLRPQAAQ"
     gene            2377..3222
                     /locus_tag="VF547_00070"
     CDS             2377..3222
                     /locus_tag="VF547_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261248.1"
                     /translation="MRWPIFAAAALGLACLGAAPAPVPSAPAARPFAPTRFSVTIQGD
                     GPDVILIPGLTGSREVWRGSVAAVPGYRYHLVQVAGFAGTPARGNARGNVVAPLADEV
                     ARYIAARGLRRPAIVGHSMGGTVAMMVAARYPARVGKVMVVDMLPEPAGIVGGDAAGI
                     RGLADSLRDLSATPGGRRLVESAIRLFGNPESNADPDVVARATHELALTDLTRELPRI
                     AAPLTVVYASLDPSRRAADDARYRAAYASRPGARLVRIDNSGHMIMYDQPAKFRTELK
                     AFLAR"
     gene            complement(3224..3895)
                     /gene="merB"
                     /locus_tag="VF547_00075"
     CDS             complement(3224..3895)
                     /gene="merB"
                     /locus_tag="VF547_00075"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEX8261249.1"
                     /translation="MAELSPEDQDLRGLLARAIARLGHAPSTAELAAEAGMEAAEVEE
                     ALRRQHEAHALLLHPHKCEPWAVHPFALSAGSCWVETAERGYWANCLYCALGIAAALE
                     ADARITTRLGGEGRTAHYRVESGRLLDSPGLFHFSTPARHWWDNVIHACATFQPFASE
                     AEIGPWCRRHALPRGHVMSLPALWRFASDWYGGCLAQPWRKRSPDEVRALFDAHGLTG
                     DFWSW"
     gene            complement(3888..5501)
                     /gene="nadB"
                     /locus_tag="VF547_00080"
     CDS             complement(3888..5501)
                     /gene="nadB"
                     /locus_tag="VF547_00080"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX8261250.1"
                     /translation="MSGTNHEFDVVVIGSGAAGLSAALTLAPKLRVAVLAKGGLSSGS
                     TALAQGGIAAVLEPGDTFESHIEDTIVAGAGLNRRETVEFVVENAPAAIDRLAALGVP
                     FNKGEAEGWHLTREGGHSHRRIVHVADATGWAVQQALEAAAADHPNISLIPDRVAVDL
                     VTGRHAEGPDAGRVWGVYALNRETGRVELFTGRATVLATGGASRAWLYSTNPRGSTGD
                     GIAMAWRAGCRVANMEFNQFHPTCLYHHDVKNFLITEAMRGEGGRLRLPAGAKGGGRR
                     FMKRFDPREELAPRDIVARAIDHELKRLGLDFVHLDISHRDPDFIRAHFPTIAARLAE
                     PDIGIDITREPIPVVPAAHYTCGGVITDLDGRTDVEGLYAAGEVTMSGLHGANRLASN
                     SVLECLVFGVAAAEHINSRWDGLAPPPAIRPWDESRVTDSDEEVVVHHNWREIRRFMW
                     DYVGIVRTTKRLERALNRINLLKQETRDYYRHFRVTPDLVELRNLVEVAELIVRSALT
                     RQESRGLHYTLDYPDLLPEAVDTVLVPHG"
     gene            complement(5625..6557)
                     /locus_tag="VF547_00085"
     CDS             complement(5625..6557)
                     /locus_tag="VF547_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8261251.1"
                     /translation="MTASAISIVDLEKTYAGGKRALEGVSLEVPRGEIFGLLGPNGAG
                     KSTLINILAGLVNKSGGSVSIWGFDIEKHPRNAKVSIGIVPQELVFDPFFTPRETLEI
                     QAGLYGVPKARRRTLELLRAVRLEDKAEAYSRTLSGGMKRRLLVAKAMVHSPPILVLD
                     EPTAGVDIELRQQLWAYVRQLNAEGVTVVLTTHYLEEAEQLCDRIAIINHGRLIANET
                     TRTLVGMAQEKAVEVTVDRDIAALPDASCFEKIDLMGERTLAITYRKDKVNAGEVLAA
                     LQRDGLGIVDVSTREADLEDVFLNLTRASDAPAG"
     gene            complement(6666..8696)
                     /locus_tag="VF547_00090"
     CDS             complement(6666..8696)
                     /locus_tag="VF547_00090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX8261252.1"
                     /translation="MTAAIIDDSFASTIPAFAGRVHPASQDVVASRGLDGEHHHGTPV
                     ASVLGASKDDSGMHGVAFNSTLLMLRTDAPGTCSANSPTCQFNHADLAKAYDLAIQNG
                     ARVINMSMQITPMPAILAEAIGRATAAGIVVILPAGNTFPAGGPEPFASAMIATKPEG
                     GGAVIIAGATNNAGTDLASFSHRAGSGADHYLAAVGEGMWVYNRNGGISTGAIGTSFS
                     APVIAGAVALLAEAFPNLTGQQIVKLLLTTATDMGAPGTDNIYGRGALNLANAFAPQG
                     ALSLAGSEAPLSLTSNGVLSPAMGDAKAGSAGAVFLDGYKRAYETDVGRTLSRGAPYA
                     PLHAALDGRYSTDSTALGPLAVSVTTRLASHGPAEAQLQPMELSHEQARAAKVMAATA
                     VGRLSPKTAVALGLSEGGKALQQRLSGQADRAFLVARDPAGDNGLHARAGSSIGLRHD
                     FGPLALTVTSEAGEVDDPYPAQRSARSGYRSTALVAERRLGRIRLSLGGSLLDEEATI
                     LGGRFSPAFSTAGSASWFADGSASFYLGGGWGASASYRHGWTSIRGGDAMVQGGRLSS
                     NAFALDLTRTGALARNDWIGLRVMQPLRVRSGGLDINLPVGYDYASGRVTYQQRLLNL
                     APTGRELTYELAYGLRLWGGNMAANAFVRTDPGHIESMRNDIGGALRFTLGF"
     gene            9096..9548
                     /locus_tag="VF547_00095"
     CDS             9096..9548
                     /locus_tag="VF547_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8261253.1"
                     /translation="MDALDRSLLALLQEDSRRTADTLGETLGLSRSAITRRIQKLTQA
                     GVIAKEVAILGDAFRGNRVTAIVNVQFDRHSPSEADRFRRVIRALPEVQLFVEITGAS
                     DALLLVSVRDMDHFNIFSDELASMSFVRRYETSFVKRTLKFTTAVPLE"
     gene            9837..10589
                     /locus_tag="VF547_00100"
     CDS             9837..10589
                     /locus_tag="VF547_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261254.1"
                     /translation="MLLHGLMADSTFFERQRPLADTFRIVALDLRGHGQSPVGVAPPT
                     IRRLADDVAELAEALELDGAVGVGWSLGAAVLWRVLAGPASAKFAGSVVGVMTPRVLN
                     EDGWDLGLSSETCEARSQAIRDDFPAFAAAAGRAIFAAPGDEADRAAAAFAANDPAAI
                     DALWQSLVEEDFRPLLPSIRQPTLVVHGAQSHLYGADTAGHLVAALPNARAVAFDRSG
                     HAPHLEQAELFNATLREFAATLPPVRQPEATA"
     gene            10609..13074
                     /locus_tag="VF547_00105"
     CDS             10609..13074
                     /locus_tag="VF547_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8261255.1"
                     /translation="MTRLSTRTLLRLGAAAAALAAVPAPLYAQSEDPPAGATEEDAAA
                     AANEALDEAATAVPSEDADIVVTARRRAESLQDVPIAVTAYQGEALERAGATDITDLS
                     DTTPNVTLETSRGTNTTLTAFIRGVGQQDPVAGFEAGVGLYLDDVFLNRPQAAVLDIY
                     DVERIEVLRGPQGTLYGRNTIGGAIKYVTRRLSDLPTLNARVNLGTYNQIDVVVSGST
                     PIVPGLKVGASAARLSRGGFGDNLNIPGLENYNRDIWAYRATIEMEPTDNFTIRFSAD
                     HTNDNSDPRNGHRLIPSLLTGAPVLADVYDTRAGLNTPEQDIEAFGGSMHAELKLSDS
                     ITIRDIAAYREDRSATPIDFDALPTQDVDVPAIYSNHQFSNELQLLYEGEKLSGVAGF
                     YYLNAEARNIFDVILATTGALIGVPGFTASTFGEVHTKTWAAFADFSYRFTDQFQLSL
                     GGRYTSDNRRSRVIRANLRGGPSPELGGGPINGIFNAVTNPGGRQFGALTSDFSGEKT
                     FKEFTPRASVSFQPNDDHTFYASWSKGFKGGGFDPRGLSTAAPDLNGNGVREADEIFD
                     YFLFDPEKVTSYEIGYKASLFDRRLRLALAGFYADYKDVQVPGSVGAVINGVPTFVGV
                     TTNAGKATFKGVEVEALATLFRGEEGSRLTFAGTLGYLDAQYDEFITNVPGTGPVDVA
                     DFRRIQNTPKWTLSGTLDFSTPLAGGTADINTTLSYRSKTFQFETPSPFLDQEGYALW
                     DANFVWTSRSDRWSIGVHAKNILDKQYITSGYQFLSVNPVTGVPNVNPVTGNVIPSLG
                     REGVVTTFYGNPRQVFLSLGMKF"
     gene            13251..14573
                     /locus_tag="VF547_00110"
     CDS             13251..14573
                     /locus_tag="VF547_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261256.1"
                     /translation="MNAGSSGGGRVLAILLIAYIFNFVDRQIIGILAVPIKAELQLSD
                     TQLSLMGGIAFALFYSGLAIPIAWLADRRSRVNIIAFSVGLWSLFTAACGLAQNFWHL
                     FLARMGVGIGEAGGVAPSYALVSDYFPKEKRARALAFFSLGIPIGSALGVFFGGWIAS
                     HLDWRSAFVIVGLAGIPAALLVKLGIREPVRGGFDSADGRASEPAPPFPAVAATLART
                     PSFWLLSFGAASGSILGYGLIFWLPSFFSRSFDFAGMDPILRALPGWFTGGADPKLVR
                     VSIFYGSIVLVGGVAGTWLGGWLADRTGGDRPASYALIPACCFVIAAPAFALGLFAPS
                     LLVGWLLFTLGQMLALAWLGPVIAAVQHIVPPNMRATASASFLFINNLIGIGFGIFFL
                     GFMSDRMTAAHGVDALQYSILYGLGFYLLSALLYFLAAGRLKRDWYRP"
     gene            complement(14743..17508)
                     /locus_tag="VF547_00115"
     CDS             complement(14743..17508)
                     /locus_tag="VF547_00115"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX8261257.1"
                     /translation="MPTRFASVPGKRAIVDRRALADALRAAPDAAAAILKRGLGAGRA
                     EIARRLEDQPYAGTEAAAAYAFLTDQIVRLAYDHETAALPPPSRSDRLLLLAVGGYGR
                     GEMALHSDVDIAFVTPGKPGRWAGQAVEALLYRLWDLGLKLGPSTRSADELIALASAD
                     HTVRTALLEARWLWGDEPLYGEVQGRFWKEVVAGQAKSFVTEKLEERERRHRRMGDSR
                     YVVEPNVKEGKGGLRDLHTLFWIGKYAYQVTTVADLVGVGLLSATELRQFQRAERFLW
                     AVRCHLHLVAGRAEERLTFDYQREIAARMRYADRPGKSPVERFMRHYFLHAKTVGDLT
                     GVFLAHLDDKFAKKGGRFGLPAIRRRPSRLEGFVLERGRLALPGDGFFRDDPVRLLQL
                     FALADRYELEIHPLAMRAAARDAKLVDQEVRSDPRANALFLDVLTSPRDPERVLRWMN
                     EAGVFGRFVPDFGRVVAQMQFDMYHHYTVDEHSIRAIGLLSRIEKGEMKADHPLATAL
                     FRHIASRRVLYAAVLLHDIAKGRGGDHSLLGAEVARALCPRLGLTEAETETVAWLVRW
                     HLLMSATAFKRDLADPQTIHDFAQRVKSLERLRLLFILTVVDIQAVGPGVWNGWKRQL
                     LRELFEAAEEALRLGHKQKGRSERIAAMQAELAGRLGWEDSRFARHAWRLPDSYWLAE
                     PLEVLERNAALMDSADRAPSRTRPLAVSVEDERGATLLCVYAKDQPGLFYRLSAAISL
                     AGANIIDARIHTTLDGMAVDNFLVQDSKGGPFADRHQLKRLEEGVLRAIDGQEPAMER
                     LEAKALPLLRAEAFAIQPAVFVDNEASSRYTVVEVNARDRAALLGSLARALYESKAAI
                     HSAHIATYGERAVDVFYLTDLHGAKIDSAARLKALRSALLKAAAETGAQDRHPSSPPR
                     PRATSNA"
     gene            complement(17508..20090)
                     /gene="mutS"
                     /locus_tag="VF547_00120"
     CDS             complement(17508..20090)
                     /gene="mutS"
                     /locus_tag="VF547_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX8261258.1"
                     /translation="MATQAKTGSAPTPMMAQYLALKAEAPDCLLFYRMGDFFELFFDD
                     AKAAARCLDIALTARGEHEGEPIPMCGVPIHAAEAYLARLIKGGFRVAIAEQVESPAE
                     ARKRGAKSVVARAIVRVVTAGTLTEEALLDSRAANWLVAVARVGERFGLAAADISTGR
                     FELGSVPPHALDSELARLAAAEVIDQERRSGFDSIAAEKRLCERFEVATLDGFGAFDR
                     AALCAAGGLLAYLDDVAKESLPFLRPPVARSSGDHMMIDAATRESLELTQSAAGGRAG
                     SLLHSVDRTVTGAGARLLAADVGAPLLDRPAIEARLDLVALFEGDAGLRDMLRRQLKA
                     LPDIGRALGRLVAGRGGPRDLGQLRDGLNETRRLHARLAALADPPPLLASLLPALTGH
                     GALTDLLDRALVPSPPIDAAQGGYIAEGYDSALDDLRATGGEGRRAIAGLEARYRQQT
                     GIATRKIRHNGVLGFHIEVPAKHADPLMAADSGFTHRQTLAGVVRFNAPDLHEQAMRV
                     GQAGAHALAAEASHLEELTQAALARAEPIAAAADALARLDVAAALAERAAEGGWARPR
                     FAGHNCFDVEGGRHPVVEDSLRASGARFVANDCALSEEERLWLVTGPNMGGKSTFLRQ
                     NALIAVLAQAGSYVPAERATLGLVDRLFSRVGASDNLARGRSTFMVEMVETAAILSQA
                     TERSFVILDEVGRGTSTYDGLAIAWAVTEAIHDRNRCRCLFATHYHELTRLAERLDAL
                     SLHHVRAREWKGDLVLLHEVAKGPADRSYGLAVAKLAGLPAPVLARARAVLAKLEQGR
                     AATGGIAAGLDDLPLFAATAQQEEPAPDPLQAALAGVDADSLSPREALDLVYRLKRLA
                     AGED"
     gene            complement(20150..20674)
                     /locus_tag="VF547_00125"
     CDS             complement(20150..20674)
                     /locus_tag="VF547_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261259.1"
                     /translation="MSRPASLALFVLLAAAAVLAPLLERPGPPSNGPPAPWPDSLEGR
                     PLIALPAAPEDRLLARRFPGHVARFSDGRRQIVLRQVAAATRRLHPASDCFRAVGYEI
                     GPSPMRMIPGAGPASCFTARRGGRTLRACEQILGSDGRSWPDISSWYWPALVGSSRGP
                     WLAVLTVEPVAATS"
     gene            complement(20671..21465)
                     /locus_tag="VF547_00130"
     CDS             complement(20671..21465)
                     /locus_tag="VF547_00130"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HEX8261260.1"
                     /translation="MPPGLDPRLALVLVPCLFWDAWRLLAGRLGDGLSAVPLALVGLA
                     VATPAIRRIAAGPARPVPLAQVSVALGLYVVATLLAPPLARVAVAVVAVLLLCRRVAG
                     GGLAWPPLVGLALLALPVFPSLDFYLAYPMRLVGAELAAGLLRLNGIAASVDGVALSW
                     NGSLLLFDPACSGLRMLWASLFLVSGVALAAGFGAIRYARAIAIAVALATVGNAVRAA
                     SLFYVENGFVEGLGGPAAHEAVGVVSFLMLAIALLACVAPRLPRSA"
     gene            complement(21465..>22168)
                     /locus_tag="VF547_00135"
     CDS             complement(21465..>22168)
                     /locus_tag="VF547_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX8261261.1"
                     /translation="PWSPAQRARAEQALADTAFDGGEDNLPLLAEAIDAAAGGEAVLL
                     WIHGPQPVAFARSGARLQPLLERSAALPRLVRYQAETGPAFAAGHAWFDTAREVAPSG
                     DARADLAALLGDLAGGGERWEVTRRQSAEGGGPTGSAHIARLWGAERLAGAGQVRGAE
                     RGRAIALAHRLNIVTPVSGAVVLETDAEYERSGLAVPSAGDVPTVPEPATWALIAIVA
                     ALLLWQLRRRRLVPA"
BASE COUNT         3316 a         7673 c         7835 g         3344 t
ORIGIN      
        1 gtcgagatgc tttctggcga gatcgtccat cgcatggctg gcgaggccgc cggcatcgag
       61 gttgaagctc atcaccaggc tgtcgtccac cggcgcgacc ttgattccgc ggcggtcgaa
      121 gacgatccag tcgaacttga aattgtggcc gatcttgagg acggccgggt cctcgaacag
      181 cggcttcagc ctcgccagcg cgacctccgc ccggatctgc tcgggccgct cggacagcag
      241 gtcatgcccg ccatgctcga gcgggacgta gcaggccctg ccgcattcgg tggcgaggct
      301 gatgccgacc agggtggcgg tgacgcagtc atagccgtcg gtctcggtgt ccacggcgac
      361 caggccgttg gccctggcct ccgcgatcca gcggtcgagc tccgcctcgc tcgtcaccgt
      421 ggcgtaggcg gagcggtcga tcttcggctc cggccggacc tcgcgctgaa ccggcgcggc
      481 cgagtcgctc ggcgccttga cctgggaggt gccgttcagg cgggcgagca gcgccttgaa
      541 gccctgctcc tcgaggaagg cgcgcaacgg gccgtccggt atgcccttca tctcgagatc
      601 gccgagcggc tcgggaagcg gcgcgtcgca caccagggtg acgagctcgc gcgagaggcg
      661 ggcggcgtcg gtgtggagaa gcaggttctc cctcagcttc ggcttcttga tctcctccgc
      721 atgggcgagc acgctttcga ggtcgccata ttcctggatg agctgggccg cggtcttggg
      781 cccgatcccc gggactcccg gcacgttgtc gacgctgtcg cccatcaggg cgaggacgtc
      841 gcccagcttc tccggcggca ctccgaactt ctcgatcacg tagtcgcgcc ccaggcgccg
      901 gtcgttcatc gtgtcgtaga ggtcgagccg gccgtcctcg accagctgca tcaggtcctt
      961 gtcggaggag acgatcgtga ccttccagcc ctgctcgagc gccgccttcg cgtagcaggc
     1021 gatgatgtcg tcggcttcca gcccctgctc ttcgatgcag ggcagggaga aggctcgggt
     1081 ggcgtcgcgg atcagcggga attgggggac gagatcgggc ggcggcggcg gccggttggc
     1141 cttgtactgg tcgtagcgct cgttgcggaa ggtcttctcg ccggcgtcga agatgacggc
     1201 gaggtgggtc ggcccctcct ccttgtggag gctctcggcc agcttccaga gcatggtggt
     1261 gtagccgtag acggcgccga cgttgagccc gtgccggttg gtgagcggcg gcaggcggtg
     1321 ataggcgcgg aagatgtagc tggatccgtc gaccagatag aggtggggca tcgggaggct
     1381 ctagcgcagc cggagcggtg gacaaagaat ctttggccgg cgcgcgggac ggggattcta
     1441 cggtctggga ttcgttaacc atcgcgacct agcatcagcc gcatgagtcg cggcctgctc
     1501 ccgctttcct gtctcgccct cgcgcccttc gcggcgacga ccgccgcggc ggcgcctttc
     1561 gagccgacgc gattctcggt ggcggtgcgg ggcaccggac cggacgtgat cctgataccc
     1621 ggcctggccg cgtcgcgcgg catctggaat tccaccgccg ccgccgtgcc cggctaccgc
     1681 tatcatctcg tccaggtcgc cggcttcgcc gggacggccg ccgccggcaa taggtcggga
     1741 ccggtggccg ccgccgtcgc ggacgagatc gcgcgctacg tcgtcgcgaa tcggctcggc
     1801 cggccggcga tcgtcggcca ctcgatgggc gggtcgatcg ccctgatgat cggcgcgcgg
     1861 catccgcagt cggcaggcag ggtgatggtc gtcgacatgc tcccctcacc ggcccgcccg
     1921 ctcgggatcc ccgcgctggt gatgaagccg ctggccaggc tggtcggtgg cgagatgcag
     1981 cgcgccagcc gcttccggct gcggatcaag ccgctcgtcg agcggttcgg caatgcggac
     2041 tggctggaga gccgcagcga cggcgacgtg atcggccgat cggtcgagga gttgatggcg
     2101 accgatctca cgccggagct gagccggatc cgagcgccgc tgacggttgt gtacgcctgt
     2161 tcgccgaccc tgggctcggc ctgcggccgg gtgagccgaa tctatgccga cgcctatgcc
     2221 gcccgccccg gaaccaggct ggtgcggatc gaccgcagcg ggcacaccat catgctggac
     2281 cagccggccg ccttccaggg cgcgctgcgc gcgttcctgc gtccgcaagc cgctcagtaa
     2341 gcgcaggttc aggccggggc cgccagaagc cgcgccatga gatggccgat cttcgccgcc
     2401 gccgccctgg gactggcctg cctcggcgcg gcgccggctc cggtcccgtc cgccccggcg
     2461 gcgcggccgt tcgcgccgac gcgcttctcg gtgacgatcc agggcgacgg ccccgacgtc
     2521 atcctcatcc ccggcctcac cggctcgcgc gaggtctgga ggggcagcgt cgccgcggtt
     2581 ccgggctatc gctaccatct cgtccaggtc gcgggcttcg ccggcacgcc cgcgcgcggc
     2641 aacgccaggg gcaatgtcgt cgcaccgctc gccgacgagg tcgcccgcta catcgcggcc
     2701 cgcgggctcc ggcgcccggc gatcgtcggc cattcgatgg gcggaacggt ggcgatgatg
     2761 gtcgccgcac gctatcccgc ccgcgtcggc aaggtcatgg tggtcgacat gctgccggag
     2821 ccggccggga tcgtcggcgg cgacgcagcc gggatacgcg gcctcgccga cagcctccgc
     2881 gacctcagcg ccacgccggg cggccgccgg ctggtcgagt cggcgatacg gctgttcggc
     2941 aatccggaga gcaatgcgga cccggacgtc gtcgcccgcg ccacccacga gctcgcgctg
     3001 accgacctca cccgcgagct gccccggatc gccgcgccgc tgaccgtcgt ctatgcgagc
     3061 ctcgatccga gccgccgggc cgccgacgat gcgcgatacc gggcggccta tgcgtcccgg
     3121 ccgggggcca ggctcgtccg catcgacaat agcggccaca tgatcatgta cgaccagccg
     3181 gcgaagtttc gaacggagct gaaggccttc ctcgcccgct gattcaccag gaccagaaat
     3241 cgccggtgag gccgtgggcg tcgaacagcg cgcggacctc gtccgggctt cgtttccgcc
     3301 acggctgggc gaggcagccg ccataccagt ccgaggcgaa gcgccacagc gccgggaggc
     3361 tcatgacatg gccgcgcggc agggcatgcc gtctgcacca cgggccgatc tcggcttcgg
     3421 aggcgaaagg ctggaaggtg gcgcaggcgt ggatgacatt gtcccaccag tggcgggcgg
     3481 gggtcgagaa atggaacagg ccgggggagt cgagcagcct tccgctctcg acccgatagt
     3541 gcgccgtccg cccctcgccg cccaggcgcg tggtgatccg cgcgtcggcc tcgagcgccg
     3601 cggcgattcc gagggcgcaa tagaggcaat tggcccagta gccgcgctcc gccgtctcca
     3661 cccagcacga ccctgcggac agggcgaacg ggtgcaccgc ccagggctcg cacttgtgcg
     3721 ggtgcagcag aagtgcgtgc gcctcgtgct ggcgccgcag cgcttcctcg acctcggccg
     3781 cctccattcc ggcctcggcc gcgagctcgg cggtcgaggg cgcgtggccg aggcgggcga
     3841 tcgcgcgagc gaggaggccg cgcagatcct ggtcctcggg gctcagctca gccatggggg
     3901 acgagcacgg tatccaccgc ctcgggaagc aggtcgggat aatcgagcgt gtaatggagg
     3961 ccccggcttt cctggcgggt cagggccgaa cggacgatca gctcggccac ctcgacgagg
     4021 ttgcgcagct cgacgaggtc gggggtgacc cggaagtggc ggtaataatc gcgcgtctcc
     4081 tgcttgagca ggttgatccg gttcagcgcc cgctcgagcc gcttggtggt gcggacgatc
     4141 ccgacatagt cccacatgaa acgccggatc tcgcgccaat tgtggtggac gaccacttcc
     4201 tcgtcggagt cggtgacccg gctctcgtcc caggggcgga tggcgggcgg cggcgcgagc
     4261 ccgtcccagc gggaattgat atgctcggcg gcggcgacgc cgaagacgag gcattcgagc
     4321 accgagttgg acgccaggcg gttggcgccg tgaaggcccg acatggtgac ctcccccgcc
     4381 gcatagaggc cctcgacgtc ggttcggccg tcgaggtcgg tgatcactcc gccgcaggta
     4441 taatgggcgg cggggacgac cgggatcggc tcgcgggtga tgtcgatgcc gatatcgggc
     4501 tcggcgagcc gggcggcgat ggtcgggaaa tgggctcgga tgaagtccgg gtcgcggtgg
     4561 ctgatgtcca ggtggacgaa atccaggccc agccgcttca gctcgtggtc gatggcgcgg
     4621 gcgacgatgt cgcggggcgc cagctcctcg cgcgggtcga agcgcttcat gaaccgccgg
     4681 ccgcccccct tcgccccggc gggcagcctg agcctgccgc cttcgccccg catcgcctcg
     4741 gtgatcagga agttcttgac gtcatggtgg tagaggcagg tcgggtggaa ctggttgaac
     4801 tccatgttcg ctactcggca tccggcgcgc caggccatgg cgatgccgtc gccggtcgat
     4861 ccgcgcggat tggtcgaata gagccaggcg cggctggcgc cgccggtcgc gaggacggtc
     4921 gcccgcccgg tgaacagctc gacccggccg gtttcccggt tgagcgcgta gacgccccag
     4981 acgcggccgg catccgggcc ttcggcgtgg cggccggtga ccagatcgac cgcgacccga
     5041 tccgggatca ggctgatgtt cggatggtcc gccgccgccg cttcgagcgc ctgctgaacc
     5101 gcccagccgg tcgcgtcggc gacgtggacg atccggcggt ggctgtggcc gccctcgcgg
     5161 gtcaggtgcc agccttcggc ctcgcccttg ttgaacggca cgcccaaggc cgccagccgg
     5221 tcgatcgcag cgggtgcgtt ctcgacgacg aactcgaccg tctcgcgccg gttgagcccg
     5281 gcgccggcga cgatcgtgtc ctcgatatgg ctctcgaacg tgtcgccggg ctccagcacc
     5341 gcggcgatgc cgccctgggc gagcgcggtg gacccggagg agaggccgcc cttggcgaga
     5401 acggcgaccc ggagcttcgg cgcgagcgtg agcgccgccg acaggccggc ggcccccgag
     5461 ccgatgacga cgacgtcgaa ttcgtgattt gtgccgctca ccaggcgacc ctaacccgtt
     5521 cgggctgagc ttgtcgaagc cccctccttt gcctctcgag cgcgaacagc aaggaaaggc
     5581 cgtcgacagg ctcggggcga acggagaagg tcgcttccgc cgaactagcc tgcaggtgca
     5641 tcggacgccc tggtcaagtt caggaacacg tcctccaggt ccgcctcgcg cgtcgagacg
     5701 tcgacgattc cgaggccgtc gcgctgaagc gcggcgagca cctcgccggc gttcaccttg
     5761 tccttgcggt aggtgatggc gagggtgcgc tcccccatca ggtcgatctt ctcgaagcag
     5821 gaggcgtcgg gcaaggcggc gatgtcgcgg tcgacggtga cctcgaccgc cttctcctgg
     5881 gccatgccga ccagcgtgcg ggtggtctcg ttggcgatca ggcggccgtg attgatgatc
     5941 gcgatgcgat cgcacaattg ctcggcttcc tcgagatagt gggtcgtcag gaccaccgtc
     6001 acgccttcgg cgttcaattg acgcacatag gcccagagct gctggcggag ctcgatgtcg
     6061 actccggcgg tcggctcgtc gaggacgagg atcggcggcg agtgcaccat cgccttggcg
     6121 accagcaggc gccgcttcat tccgcccgac agcgtgcgcg aataggcctc ggccttgtcc
     6181 tcgagtcgga ccgcgcggag cagctccagc gtccgccgcc gcgccttggg cacgccgtag
     6241 aggccggcct ggatctcgag cgtctcgcgc ggcgtgaaga aggggtcgaa gaccagctcc
     6301 tgcgggacga tcccgatcga caccttggcg ttgcgcgggt gcttctcgat gtcgaagccc
     6361 cagatgctga ccgagccgcc gctcttgttg accagccctg cgaggatgtt gatcagggtc
     6421 gacttgccgg ctccgttggg cccgagcagt ccgaatatct ctccccgcgg cacctcgagg
     6481 ctgacgccct cgagcgccct cttgccgccg gcataggtct tctcgaggtc gacgatcgag
     6541 atggcggagg cggtcatgag gggggcttta gcggctgaac cccctcccgc atagaagccc
     6601 gctcgccgtc acgaagtccc ttagcgtctt cggccggccc atatgtgcgc gccgtgtacc
     6661 cgagatcaga atccgagcgt gaaacggagg gcgccgccga tgtcgttcct catcgattcg
     6721 atgtggccgg gatcggtcct gacgaaggcg tttgcggcca tgttcccgcc ccacaggcga
     6781 aggccatagg ccagctcgta agtcagttcg cgcccggtcg gagcgaggtt gaggaggcgc
     6841 tgctggtagg tgaccctgcc ggacgcatag tcgtagccga ccggaaggtt gatatccaag
     6901 ccgccgctgc ggactcgcag cggctgcatg acgcgcaggc cgatccagtc gtttcgtgcc
     6961 aaggcgccgg ttctggtcag atcgagcgcg aaggcattgc tggagagccg gccgccctgg
     7021 accatggcgt cgccgcctcg gatcgaggtc cagccgtgcc ggtagctggc agaggcaccc
     7081 cacccgccgc cgagatagaa ggaggccgag ccgtcggcga accagctggc cgagccggcg
     7141 gttgagaagg cgggggagaa gcggccgccg agtatcgtcg cctcttcgtc gaggagcgag
     7201 ccgcccaggc tgagccggat ccggcccagc cgccgttcgg cgacgagcgc ggtggaccgg
     7261 tagcccgaac gcgccgaacg ctgcgccgga taggggtcgt cgacctcccc ggcttccgag
     7321 gtcaccgtca aggcgagcgg cccgaagtcg tgccgaaggc cgatgctcga gccggcgcgg
     7381 gcgtgtaggc cgttgtcgcc tgccgggtcc cgcgccacca ggaacgctct gtccgcctgc
     7441 ccggagaggc gctgctggag ggctttgccg ccttcggaca agccaagcgc caccgccgtt
     7501 ttcggcgaga ggcggccgac ggccgtggca gccatgactt tcgccgctcg cgcctgctcg
     7561 tgggaaagct ccatcggctg aagctgcgcc tcggccgggc cgtgggaggc gagcctggtg
     7621 gtgacggaga cggccagggg ccccaatgcc gtggagtcag tgctgtagcg tccgtccagc
     7681 gccgcatgca ggggggcata aggagcgccg cgggaaaggg tgcggcctac atcggtctca
     7741 taggcccgct tgtagccgtc gaggaagacg gcgcctgcgg aacctgcctt cgcatcgccc
     7801 atcgcgggcg acaggacgcc gttgctggtg agcgatagcg gtgcctccga acccgccagc
     7861 gacagggcgc cttgcggcgc gaaggcgttc gcgaggttaa gagcgccccg gccataaata
     7921 ttgtccgtgc cgggagcgcc catgtccgtg gcggtcgtca ggagcagctt gacgatctgc
     7981 tgcccggtca ggttggggaa cgcctccgcg aggagggcga cggcgcccgc tatgacgggc
     8041 gccgagaacg acgtcccgat tgcgccggtc gaaatccctc cattccggtt atagacccac
     8101 atcccttccc cgaccgcggc aaggtagtgg tcggcgccgc tgcccgcgcg gtggctgaac
     8161 gacgcgaggt ccgtgccggc attgttggtg gcgccggcaa tgatcacggc accgccccct
     8221 tcgggtttcg tcgcgatcat cgcggatgcg aagggctcgg gccctcccgc tggaaaggtg
     8281 tttcccgccg gaaggatgac gacgatacca gcggccgttg cccggccgat cgcctcggca
     8341 aggatagcgg gcatcggcgt tatctgcatc gacatgttga tgacgcgtgc gccgttctgg
     8401 atggccagat cgtacgcctt ggccaggtcg gcgtggttga actgacaggt gggagagttg
     8461 gctgagcacg ttccaggggc atccgttctc agcatcagca gcgtggaatt gaaggctacg
     8521 ccgtgcattc cggaatcgtc cttcgacgct cccaatacgg aagccacggg cgtgccatgg
     8581 tggtgctcgc cgtccaggcc gcgcgatgcc acgacatctt gcgacgccgg atggacgcga
     8641 cccgcgaacg cggggatggt cgaggcgaaa ctgtcgtcga ttatggcggc ggtcacgccc
     8701 tttccggtcg cacctttctc ataggcgtgg atcgcatagg cccgcgctgc tcccgccgaa
     8761 ttcttatact cctgagtgtc gaatcccgga tctccggaag ggggaggggg tggcggcggc
     8821 gggggtggag ggggcggagg cggaggtgca gggggcggcg gcacgggctg gacgccgccg
     8881 cctccgccgc cgcaggcgcc aagagcgatg acgagcactg acaggctgac ggtgcggagg
     8941 atcgagcttt tcatcgccat acgcttcgtt cgaggacctg gctgcgggat ttttctttcc
     9001 gatgctaacg ccgcaacggc acatcctcgg accgattatg gcgcagccag cgcatttcga
     9061 ttgcatgagc acctagccgg cgcacattat gtgatatgga cgccctcgac cgctccctat
     9121 tggcccttct tcaggaggac agccggcgga cggccgacac gctcggggag acgctgggcc
     9181 tttcgcgctc cgcgatcacg cggcggatcc agaagctgac gcaggcgggc gtcatcgcga
     9241 aggaggtcgc gatcctcggc gacgcctttc ggggaaaccg ggtgacggct atcgtcaacg
     9301 ttcagttcga tcggcattcg cccagcgaag ccgaccggtt caggcgcgtg atccgggccc
     9361 tccccgaggt ccagctcttc gtggagatca cgggcgcgag cgacgcgctt ctactcgtgt
     9421 ccgtcaggga catggatcac ttcaatattt tctccgacga gctggcaagc atgtccttcg
     9481 tccgccgcta cgaaaccagc ttcgtcaagc ggaccttgaa gttcacgaca gccgtccccc
     9541 tcgaatagtc ctggcgccgg cttcatcatt ccgcacgaca aagaggatag ccgttgcggg
     9601 ccccttgagg gccgctgacg tcggccaatc gacctggcag aggtgcgcga acaaaggact
     9661 acccctctcg gcaacgtact tgaacattga ctcaggtttc agtttcaggc atggcctcgc
     9721 gtgtcgccac aagactccgt ccaaggagcg cgcggccggg ggagaggttg aggcggttca
     9781 cggcatccga cggcgagcgt atcgcttatt ccgatgaggg cggggggcgc ccgctcgtgc
     9841 tcctgcacgg gctgatggcg gattccacct tcttcgagcg gcagcgtccg ctcgccgata
     9901 cgttccggat cgtcgccctc gacttgcgcg gccacggaca atcgcccgtc ggcgtggcgc
     9961 cgccgacgat ccggcggctt gcggacgacg tggccgaact ggccgaggcg ctggagctgg
    10021 acggcgccgt cggcgtcggc tggtcgctcg gagccgcggt gctctggcgg gtcctcgccg
    10081 gccccgcttc ggccaaattc gccggctcgg tggtcggcgt catgacgccg cgggtgctca
    10141 acgaggacgg ctgggacctc ggcctgtcga gcgagacctg cgaggcgcgc agccaggcca
    10201 tacgggacga tttccccgcc ttcgccgccg ccgccggccg ggcgatcttc gcggcgcccg
    10261 gggacgaggc ggaccgcgcc gcggccgcct tcgccgcgaa cgacccggcc gccatcgacg
    10321 cgctctggca atcgctggtc gaggaggatt tccggcctct gctgccttcg atccggcagc
    10381 ccactctggt cgtccacggc gcccagagcc atctctacgg cgccgacacc gccggccatc
    10441 tcgtcgccgc cctgcccaat gcgcgggccg tcgccttcga ccgctccggc catgctccgc
    10501 atctcgagca ggccgaattg ttcaacgcca cccttcgcga attcgcggcg accttgccgc
    10561 cagtccgtca acccgaagcc accgcctaga atgccaggag aaaccgccat gacccgcttg
    10621 tccacccgca ccctgctgag gctcggcgcc gccgcggccg cgctcgccgc cgtccccgct
    10681 ccgctctacg cccagtcgga ggatccgccc gccggcgcca ccgaggagga cgcggccgcc
    10741 gccgccaacg aagcgctcga cgaggccgcc accgccgttc cgagcgagga cgccgacatc
    10801 gtcgtcaccg ctcggcgccg cgcggagagc ctgcaggacg ttccgatcgc ggtcaccgcc
    10861 tatcagggcg aggcgctgga gcgcgccgga gcgaccgaca tcaccgacct ttcggacacg
    10921 acccccaacg tcacgctgga gacgtcgcgc ggcaccaaca ccaccctcac cgccttcatc
    10981 cgcggcgtcg gccagcagga tccggtcgcc ggcttcgaag ccggggtcgg cctctatctc
    11041 gacgacgtct tcctcaaccg cccgcaggcg gcggtgctcg acatctacga cgtcgagcgg
    11101 atcgaggtgc tgcgcggccc gcagggcacg ctctacggcc gaaacacgat cggcggagcg
    11161 atcaaatacg tcacccgccg tctctccgac ctgccgacgc tgaacgcgcg ggtcaatctg
    11221 gggacctaca accagatcga cgtcgtcgtc tccggcagca ccccgatcgt tcccggcctg
    11281 aaggtcggcg cctccgcggc gcggctgagc cgcggcggct tcggcgacaa tctcaacatc
    11341 cccgggctcg aaaattacaa tcgcgacatc tgggcctatc gggccaccat cgaaatggag
    11401 ccgaccgaca atttcacgat ccgcttctcc gccgaccaca ccaacgacaa tagcgatccg
    11461 cgcaacgggc accggctgat cccgagcctg ctcaccgggg cgcccgtcct ggccgacgtc
    11521 tacgacaccc gcgccggcct caacacgccc gagcaggaca tcgaggcctt cggcggttcc
    11581 atgcatgccg agctgaagct gagcgattcg atcaccatcc gcgacatcgc cgcctatcgc
    11641 gaggaccgca gcgccacgcc gatcgacttc gacgcgcttc cgacccagga cgtcgacgtc
    11701 cccgcaatct acagcaacca ccaattctcc aacgagctgc agctgctcta cgagggcgaa
    11761 aagctcagcg gcgtggcggg tttctactat ctcaacgccg aggcgaggaa catcttcgac
    11821 gtcatcctgg cgaccaccgg cgcgctgatc ggcgtgcccg gcttcaccgc gtccaccttc
    11881 ggcgaggtcc acaccaagac ctgggccgcc ttcgccgatt tcagctaccg cttcaccgac
    11941 cagttccagc tctcgctcgg aggccgctac accagcgaca accgccggtc gcgagtgatc
    12001 cgcgccaacc tgcgcggcgg cccctcgcca gagctcggcg gcggtccgat caacggaatc
    12061 ttcaacgcgg tcacgaaccc gggcgggcgc cagttcggcg ccctgacgtc cgacttcagc
    12121 ggcgagaaga ccttcaagga attcacgcct cgcgcctcgg tcagcttcca gcccaatgac
    12181 gatcacacct tctatgcgag ctggtcgaag ggcttcaagg gcggcggctt cgacccgcgc
    12241 ggcctgtcga ccgcggcgcc ggacctgaac ggcaacggcg ttcgcgaggc ggacgagatc
    12301 ttcgactact tcctgttcga tccggagaag gtcaccagct acgagatcgg ctacaaggca
    12361 agcctgttcg accgccgcct gaggctcgcc ctcgcaggct tctacgccga ctataaggac
    12421 gttcaggtgc cgggctcggt cggcgcggtg atcaacggcg tgccgacctt cgtcggggtg
    12481 acgaccaacg ccggcaaggc gaccttcaag ggcgtcgagg tcgaggcgct ggcgaccctg
    12541 ttccggggcg aagagggctc gcgcctcacc ttcgcgggga cgctcggcta tctcgatgcc
    12601 caatatgacg agttcatcac caacgtgccc ggcaccggcc cggtcgacgt cgccgacttc
    12661 cgtcggatcc agaacacgcc gaaatggacc ctgtccggga cgctcgattt ctcgaccccg
    12721 ctcgcgggcg gcacggccga catcaacacg acgctctcct accggtcgaa gaccttccag
    12781 ttcgagaccc cgagcccctt cctggaccag gagggctatg ccctgtggga cgccaatttc
    12841 gtctggacga gccgcagcga ccgctggtcg atcggcgtcc acgccaagaa catcctcgac
    12901 aagcagtaca tcacgtcggg ctaccagttc ctgtcggtca acccggtgac cggcgtgccg
    12961 aacgtcaacc cggtcacggg caacgtcatc ccgagtctcg gccgggaggg cgtggtcacc
    13021 accttctacg gcaatccacg acaggtcttc ctcagcctgg ggatgaagtt ctaggggcca
    13081 ttcccaattc ctcccctgca tcggcagggg agggcgacca tgcgaagcat ggtggggggg
    13141 ccttgctgaa tccgtagaac ccctccaccg cgcttcgcgc ggtcccccgc cccgggaaat
    13201 ctcggggagg atttgagaaa aggcagcgaa tgtcgacacg gccggcccag atgaacgcag
    13261 gcagctccgg cggcggccgc gtgctcgcca tcctcctcat cgcctacatc ttcaacttcg
    13321 tcgaccgcca gatcatcggc atcctcgccg tgccgatcaa ggcggagctg cagctcagcg
    13381 acacccagct cagcctgatg ggcggaatcg ccttcgcgct cttctattcc ggcctcgcca
    13441 tcccgatcgc ctggctcgcc gaccgccgga gccgggtcaa catcatcgcc ttctcggtgg
    13501 gcctgtggag cctgttcacg gccgcctgcg ggctcgccca gaatttctgg catctgttcc
    13561 tggcgcggat gggcgtcggg atcggcgagg cgggcggcgt ggcgccctcc tacgcgctcg
    13621 tctccgacta tttcccgaag gagaagaggg cccgggccct ggccttcttc tcgctcggca
    13681 taccgatcgg ctcggcgctc ggcgtcttct tcggaggctg gatcgcgagc cacctcgact
    13741 ggcgctcggc cttcgtcatc gtcggcctcg ccggcatccc cgccgctttg ctggtcaagc
    13801 tcggcataag ggagccggtg cgcggcggct tcgacagcgc cgacggacgc gcgagcgagc
    13861 cggcgccgcc cttccccgcg gttgccgcca ccctggcgcg caccccgagc ttctggctgc
    13921 tctccttcgg ggcggcgtcg ggctcgatcc tcggctatgg cctgatcttc tggctgccga
    13981 gcttcttcag ccgaagcttc gatttcgccg gcatggaccc gatcctccgg gcgctgccgg
    14041 gctggttcac cggcggggcg gacccgaagc tggtccgggt gtcgatcttc tacggctcga
    14101 tcgtcctcgt cggcggagtc gccggcacct ggctcggcgg ctggctcgcc gaccgcaccg
    14161 gcggcgaccg gcccgcctcc tacgcgctga tcccggcctg ctgcttcgtg atcgccgcgc
    14221 cggccttcgc gctgggcctg ttcgcgccgt cgcttctggt cggctggctg cttttcacct
    14281 tgggccagat gctggctctg gcgtggctcg ggccggtgat cgcggccgtc cagcatatcg
    14341 tcccgcccaa catgcgggcg accgcctcgg cgagcttcct gttcatcaac aacctcatcg
    14401 ggatcggctt cgggatcttc ttcctcggct tcatgtcgga ccggatgacg gcggcccacg
    14461 gcgtcgacgc cctccaatat tcgattctct acgggctcgg cttctatctt ctcagcgccc
    14521 tgctctattt cctcgccgcc ggccgactga agcgcgactg gtaccgcccc tgaatccgaa
    14581 atccgcgact tcggtgccag gcaccgaact ctaagcgttt gatattgcgc ggggaggcct
    14641 gggcggcgag gcgcctgtat gcgaaatccg cgacttcggg tgccaggcac tgagggctac
    14701 gctgtatccg aattccgcga cttcggtgcc aggcaccgag ggctaagcgt ttgatgttgc
    14761 gcgggggcgg ggcggcgagg acgggtggcg gtcctgggcg cccgtttcgg cggcggcctt
    14821 gagcagggcc gatcgcagcg ccttgagccg ggccgcgctg tcgatcttgg cgccgtggag
    14881 gtcggtcaga tagaagacgt cgaccgcgcg ctcgccatag gtggcgatgt gggcggagtg
    14941 gatcgcggcc ttcgattcgt agagcgcgcg cgccaggctg ccgagcagag cggcgcggtc
    15001 gcgggcgttg acctcgacca ccgtgtagcg gctcgacgcc tcgttatcga cgaagaccgc
    15061 cggctggatc gcgaaggcct cggcgcgaag cagcggcagg gccttggcct cgagccgctc
    15121 catcgccggc tcctgcccgt cgatcgcgcg cagcaccccc tcctcgagcc gcttcagctg
    15181 gtggcggtcg gcgaagggcc cgcccttcga atcctggacg aggaaattgt cgaccgccat
    15241 tccgtccagg gtggtgtgga tccgggcgtc gatgatgttg gcgccggcga ggctgatcgc
    15301 ggcgctgagc cggtagaaca ggccgggctg atccttcgcg tagacgcaga gcagggtcgc
    15361 gccccgctcg tcctcgaccg acacggcgag cggccgggtc ctggacggag cacggtcggc
    15421 cgagtccatc agggcggcgt tcctctcgag cacctcgagc ggctcggcca gccagtagct
    15481 gtcgggaagc cgccaggcat ggcgcgcgaa gcgggaatcc tcccagccga ggcgcccggc
    15541 cagctccgcc tgcatcgccg cgatccgctc gctccggccc ttctgcttgt ggccgagccg
    15601 cagagcttcc tccgccgctt cgaacaattc gcgcagaagc tggcgcttcc agccgttcca
    15661 cacccccggg ccgaccgcct ggatgtcgac gacggtgagg atgaagagca gccggagccg
    15721 ctcgaggctc ttcacccttt gggcgaagtc gtggatggtc tgcgggtcgg cgaggtcgcg
    15781 cttgaaggcg gttgcggaca tgagcaggtg ccagcggacc agccaggcga cggtctcggt
    15841 ctccgcctcg gtgagcccga gtctcgggca caaggcgcgc gccacctcgg cgccgagcag
    15901 gctgtggtcg ccgccgcggc ccttggcgat atcgtggagc agcaccgccg cgtagaggac
    15961 gcgccgcgac gcgatgtggc ggaacagcgc ggtggcgagc ggatggtcgg ccttcatctc
    16021 gcctttctcg atccgcgaca gcaggccgat cgcccggatc gaatgctcgt cgaccgtata
    16081 atgatgatac atgtcgaact gcatctgggc gacgacccgg ccgaaatcgg ggacgaagcg
    16141 accgaagacc cccgcctcgt tcatccagcg caggacccgc tcgggatcgc gggggctggt
    16201 caggacgtcg aggaagaggg cgttggcgcg cgggtccgaa cggacctcct ggtcgaccag
    16261 cttggcgtcg cgggcggcgg cgcgcatcgc cagcgggtgg atttcaagct cataacgatc
    16321 ggccagcgcg aagagctgga gcagccggac ggggtcgtcg cggaagaaac cgtccccggg
    16381 caaagcgagt cggccgcgct ccaggacgaa gccttcgagg cggctggggc ggcggcggat
    16441 ggcgggcagg ccgaagcggc cgcccttctt cgcgaacttg tcgtcgagat gggcgaggaa
    16501 cactcccgtg aggtcgccga cggtcttcgc gtggaggaaa tagtggcgca tgaagcgctc
    16561 gaccggcgac ttccccggac ggtcggcata gcgcatccga gccgcgatct cgcgctgata
    16621 gtcgaaggtc aggcgctcct cggcccggcc cgcgacgagg tggaggtggc acctcacggc
    16681 ccacaggaat cgctcggcgc gctggaactg gcgaagctcg gtggcggaga gcaggcccac
    16741 tccgacgagg tcggcgaccg tcgtcacctg ataggcatat ttgccgatcc agaagagggt
    16801 atggaggtcc ctgaggccgc ccttgccctc cttgacgttg ggctcgacga cgtagcggct
    16861 gtcgcccatt cggcggtggc gccgctcgcg ctcctcgagc ttctcggtga cgaagctctt
    16921 cgcctgaccc gccaccacct ccttccagaa gcgtccctgg acctcgccgt aaagcggctc
    16981 gtcgccccac agccagcgcg cctcgagcag ggcggtgcgg accgtatggt cggcggaggc
    17041 gagggcgatc agctcgtcgg cggagcgggt cgaaggcccc agcttcagtc cgaggtccca
    17101 cagccggtag agcagggcct cgaccgcctg ccctgcccag cggccgggct tgcccggggt
    17161 gacgaaggcg atgtcgacgt cggaatggag cgccatctcg ccgcggccgt agccgccgac
    17221 cgcgagcagc agcagccggt cggagcggga cggcggcggc agcgccgcgg tctcatggtc
    17281 gtaggcgagg cggacgatct ggtcggtgag gaaggcgtag gccgccgcgg cctcggtgcc
    17341 ggcatagggc tggtcctcca ggcggcgggc gatctccgcg cggccggcgc ccaggccccg
    17401 cttgaggatc gcggccgcgg cgtccggcgc cgccctcagg gcgtcggcca gggcgcgccg
    17461 atcgacgatc gcccgcttcc ccgggaccga ggcgaagcgg gtcggcatca gtcttcgccg
    17521 gcggcgagcc gcttcagccg gtagacgagg tcgagcgcct cgcgcgggct gagcgaatcg
    17581 gcgtcgaccc cggccagcgc cgcctggagc gggtcgggcg ccggctcctc ctgctgggcg
    17641 gtggccgcga acaggggcag gtcgtcgagg ccggcggcga ttccgccggt ggcggcgcgg
    17701 ccctgctcca gtttggcgag gacggcccgg gcccgggcga ggaccggggc gggaaggccg
    17761 gcgagcttgg cgacggcaag gccgtagctt cggtcggccg ggcccttcgc gacttcgtgg
    17821 aggaggacga ggtcgccttt ccattcgcgg gcgcggacat ggtggagcga caaggcgtcg
    17881 agccgctcgg cgagcctcgt cagctcgtga taatgggttg cgaagaggca gcggcagcgg
    17941 ttgcggtcgt ggatcgcctc ggtcaccgcc caggcgatgg cgaggccgtc ataggtcgag
    18001 gtgccgcgcc cgacctcgtc gaggatgacg aagctgcgct cggtcgcctg cgacaggatg
    18061 gcggccgtct cgaccatctc gaccatgaag gtcgagcggc cgcgggccag gttgtcggag
    18121 gccccgaccc ggctgaacag ccggtcgacg aggccgagcg tcgcccgctc ggccgggacg
    18181 taggacccgg cctgggcgag gacggcgatc agcgcgttct gcctgaggaa ggtcgatttt
    18241 ccgcccatgt tcgggcccgt gaccagccac aggcgctcct cctcggagag cgcgcaatcg
    18301 ttggcgacga agcgggcgcc ggaggcgcga agcgaatctt cgaccaccgg atggcggccg
    18361 ccctcgacgt cgaagcaatt gtggccggcg aaacgcggcc gcgcccagcc cccctccgcc
    18421 gcccgctcgg cgagcgccgc ggccacgtcg agccgggcga gcgcatcggc ggcggcggcg
    18481 atcggctcgg cgcgcgccag ggccgcctgc gtgagctcct ccagatgcga ggcctccgcg
    18541 gcgagggcat gggcgcctgc ctggccgacg cgcatcgcct gctcgtggag gtcgggcgcg
    18601 ttgaagcgga ccactccggc cagggtctgg cggtgggtga agcccgaatc cgcggccatc
    18661 agcggatcgg cgtgcttcgc cggcacctcg atgtggaagc cgagcacgcc gttatggcgg
    18721 atcttccggg tcgcgatgcc ggtctgctgg cggtaccggg cctcgagccc ggcgatggcg
    18781 cgccggccct cgccgccggt ggcgcgcaga tcgtccagcg cggagtcgta gccttcggcg
    18841 atatagcccc cctgcgcggc gtcgatcggc ggcgacggca ccagcgcgcg gtcgagcagg
    18901 tcggtgagcg cgccatggcc ggtcagggcc ggaagcagcg aggcgagcag gggcggcggg
    18961 tcggccaagg cggccagcct ggcatgcagc cgccgagtct cgttcagccc gtcgcgcagc
    19021 tggccgaggt cgcgcggccc gccgcggccg gccacgagcc gcccgagcgc cctaccgatg
    19081 tcgggcaagg ccttgagctg gcgccggagc atgtcgcgaa gcccggcatc gccctcgaac
    19141 agggcgacga gatccagccg cgcctcgatc gccggccggt cgagcaaagg ggcgcccacg
    19201 tccgccgcga gcaggcgtgc tccggcgccg gtaacggtgc gatcgaccga gtggagcagg
    19261 ctgccggcgc gtccgccagc cgccgactgg gtgagctcga ggctctcgcg ggtcgccgcg
    19321 tcgatcatca tatggtcgcc cgaagaccgg gccaccggcg gccgcaggaa cggaagcgac
    19381 tccttggcga cgtcgtcgag ataggcgagc agcccgcctg ccgcgcacag ggcggctcgg
    19441 tcgaaagccc cgaagccgtc gagggtggcg acttcgaagc gctcgcacag gcgcttctcg
    19501 gcggcgatcg aatcgaagcc gctgcgccgc tcctggtcga tcacctccgc cgcggccagt
    19561 cgggccagct ccgagtcgag ggcatggggc gggaccgagc cgagctcgaa gcgcccggtc
    19621 gagatgtccg cggcggccag gccaaagcgc tccccgaccc tcgccaccgc cacgagccag
    19681 ttggcggcgc gcgaatcgag gagggcctcc tcggtcagcg tgcccgcggt gacgacccgg
    19741 acgattgccc gggcgacgac cgacttggcg ccgcgcttgc gcgcctcggc cgggctctcg
    19801 acctgctcag cgatggcgac tcggaagccg cccttgatca gccgcgccag ataggcctcg
    19861 gcggcgtgga tcgggacgcc gcacatcggg atcggctcgc cttcatgttc cccgcgcgcg
    19921 gtgagggcga tgtcgaggca ccgggctgcc gccttggcgt catcgaagaa caattcgaag
    19981 aagtcgccca tccggtagaa aagcaggcaa tcgggcgctt ccgccttgag cgccagatat
    20041 tgcgccatca tcggcgtggg ggcggaacct gtcttcgcct gggtggccat cggccgaggt
    20101 tagagggttg caggccgcca tggaacggcc ttctgacggt ggaggccgct cagctcgtcg
    20161 cggcgaccgg ctccacggtc agcacggcga gccaggggcc gcgcgagctg ccgaccagcg
    20221 ccggccagta ccaggacgag atatcgggcc agctccggcc gtcggagccc aggatctgct
    20281 cgcaggctcg aagcgtccgc ccgccgcgcc gggcggtgaa gcaggaggcg gggccggccc
    20341 ccggtatcat ccgcatcggc gaagggccga tctcgtaacc cactgcccgg aagcagtcgc
    20401 tcgccgggtg aagccgccgc gtcgcagcgg cgacctggcg aagcacgatc tggcggcggc
    20461 cgtcgctgaa tcgggcgaca tggcccggga agcggcgggc gagcagcctg tcctccggtg
    20521 cggccggtag cgcgatgagc ggcctgcctt ccaggctgtc cggccagggc gccggcggac
    20581 cgttgctggg agggcccggt cgctccagca ggggcgccag gaccgccgcg gccgccagca
    20641 gcacgaagag agcgagggag gccggccggc tcatgccgat ctcggtaggc ggggcgccac
    20701 gcaggccagc agggcgatgg ccagcatcag gaaggaaacc acgcccacgg cctcgtgcgc
    20761 cgccggcccg cccagccctt cgacgaagcc gttctcgaca tagaacaggc tggccgcgcg
    20821 gacggcgttg ccgacggttg ccagggcgac ggcaatggcg atggctcgcg cgtagcgaat
    20881 cgcgccgaac cccgcggcga gagccacccc gctgaccagg aacaggctcg cccacagcat
    20941 ccgaaggccg ctgcaggccg ggtcgaacag aagcagcgag ccgttccagc tcaaggccac
    21001 gccgtcgacg ctggccgcga tcccgttcag tcgaagcagg ccggcggcca gctccgcccc
    21061 caccagacgc atcggatagg cgaggtagaa atcgaggctc gggaagaccg gcagggcgag
    21121 caaggcgagg ccgaccagcg gcggccaggc aaggccgccg ccggcgaccc gccggcagag
    21181 cagcaggacg gcgaccaccg cgacggcgac ccgcgcgagc ggcggcgcga ggagggtggc
    21241 cacgacatag aggccaagcg cgaccgagac ctgggcgagc ggaaccggcc tcgccggccc
    21301 ggccgcgatc ctgcgaatcg cgggcgtggc gacggccagc ccgaccagtg cgagcggtac
    21361 cgcggaaagg ccgtccccga gccgccccgc caggagccgc caggcgtccc agaacaggca
    21421 aggcaccagg acgagggcga ggcgcggatc aagcccgggc ggcatcaggc cggaacgagg
    21481 cgacggcggc gcagttgcca cagcagcagg gcggcgacga tcgcgatcag cgcccaggtg
    21541 gcgggctccg gcaccgtcgg aacgtcgccg gcgcttggca ccgcgagacc gctgcgctca
    21601 tattcggcgt ccgtctcgag cacgaccgcg ccgctgaccg gggtgacgat gttcaggcgg
    21661 tgggcgagcg cgatcgcgcg gccgcgctcg gcgcctcgca cctggccggc cccggccagc
    21721 cgttccgcgc cccacaaacg ggcgatatgg gccgagcccg tcggtccgcc gccctcggcc
    21781 gactgccttc gagtcacttc ccaccgctcg ccgccgccgg cgaggtcgcc gagcagggcg
    21841 gcgaggtcag cccgggcgtc gcccgagggc gccacctcgc gggcggtgtc gaaccaggcg
    21901 tggccggcgg cgaaggcggg gccggtctcg gcctggtagc gcaccaggcg cggaagcgcg
    21961 gcactgcgct cgagcagcgg ctgcagtcgg gctccggagc gggcgaaagc gaccggctgg
    22021 gggccgtgga tccacagcag taccgcctcg ccgcctgccg ccgcgtcgat cgcctcggca
    22081 agcagaggca gattgtcctc gccgccgtcg aaagccgtat ccgcgagcgc ctgctcagcg
    22141 cgcgcgcgct gggccggcga ccagggcg
//
LOCUS       DASVQZ010000004         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_96426, whole genome shotgun sequence.
ACCESSION   DASVQZ010000004 DASVQZ010000000
VERSION     DASVQZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96426"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VF547_00140"
     CDS             <1..717
                     /locus_tag="VF547_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8261262.1"
                     /translation="FTGPLGAYATHRTIDADRLVKLPDAVTAEDAAAAMLKGCTAEYL
                     IERCARVEAGQAVLVHAAAGGVGAILVQWLKAIGAVVIAHAGDSRKAAMAKELGADHA
                     LCGPMEGLAAEVRALTDGKGVPAVLDGVGAASWQASLASVARRGLVVTYGNASGPVPP
                     FTAFDLLSAGSVFVTRPTLADYCKTPEEMRASAARLFEMIEKGAVKIRIGATLPLLEA
                     ADAHRALEARATMGSTVLIP"
     gene            complement(726..1370)
                     /locus_tag="VF547_00145"
     CDS             complement(726..1370)
                     /locus_tag="VF547_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671633.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HEX8261263.1"
                     /translation="MDLGSLPTTGPAAAFLQVVLIDLVLAGDNAIVVGALAAGLPAEQ
                     RRRVIMIGIAAALVLRIGFALIVTQLLQLIGLVLAGGLLLLWVAWKMYRELRHAGQSP
                     GSQEIEGDESTGLRPARSFAAAAWAVALADVSMSLDNVLAVAGAAREHPWVLIVGLVL
                     SVALMGVAANVIARYIERYRWIAWIGLAVILWVAGTMIHDGIVSPEVGVKRLFA"
     gene            complement(1413..2669)
                     /gene="rho"
                     /locus_tag="VF547_00150"
     CDS             complement(1413..2669)
                     /gene="rho"
                     /locus_tag="VF547_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX8261264.1"
                     /translation="MHLKDLKQKSPADLVAMAEELGIEGASTLRKQELMFSILKVQAE
                     NGQEIMGQGTIEVLPDGFGFLRSPEANYLAGPDDIYVSPNQVRKFGLRTGDTVEGEIR
                     GPKDGERYFALVRLAQVNYDDPEAVRHRVNFDNLTPLYPDEKLTLDSADPTIKDKSAR
                     VIDIISPQGKGQRALIVAPPRTGKTVLLQNIARAITDNHPEVFLIVLLIDERPEEVTD
                     MQRSVKGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSKMDEVIFEE
                     FKGTGNSEIVLDRKVSDKRIFPSLDVGKSGTRKEELLVDQGTLSKMWVLRRILMQMGT
                     VDAMQFLLDKMKDAKSNEDFFASMNQ"
     gene            complement(2925..3377)
                     /locus_tag="VF547_00155"
     CDS             complement(2925..3377)
                     /locus_tag="VF547_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX8261265.1"
                     /translation="MYDWGTVLTLSYAWIKAGHVIFVIFWIAGLFMLPRFYVYHQESA
                     PGSAEEARWIERERKLRRIIITPAMILVWMFGLALAWINEAWVFGWFQAKFVLVVALS
                     AYHGWMVGYGRRLARGERRLSGKGLRMMNEVPGLAVVLIVVLVIVRPF"
BASE COUNT          574 a         1099 c         1189 g          560 t
ORIGIN      
        1 ttcaccggcc cgctcggcgc ctatgcgacc caccgcacga tcgacgccga ccggctggtc
       61 aagctgcccg atgcggtcac ggccgaggac gcggcggcgg cgatgctcaa gggctgcacg
      121 gccgaatatc tgatcgagcg ctgcgcccga gtcgaggcgg gccaggcggt gctcgtccac
      181 gccgccgcgg gcggggtcgg agcgatcctg gtccagtggc tgaaggcgat cggagcggtc
      241 gtgatcgccc atgccggcga cagccgcaag gcggcgatgg cgaaggagct cggcgccgat
      301 catgcgcttt gcggcccgat ggaaggcctg gccgcggaag tgcgcgcgct gaccgacggc
      361 aagggcgtgc cggcggtgct cgacggggtc ggcgcggcga gctggcaggc ctcgctcgcc
      421 tcggtcgccc ggcgcgggct cgtggtgacc tacgggaacg cctcggggcc ggtgccgccc
      481 ttcacggcat tcgacctgct cagcgccgga tcggtgttcg tgacccggcc gacgctcgcc
      541 gattattgca agacgccgga ggagatgcgg gcctcggcgg ctcggctgtt cgagatgatc
      601 gagaaggggg cggtgaagat ccggatcggc gccaccctgc ccctcctcga ggcggccgat
      661 gcgcaccgcg ccctggaggc gcgggcgacg atgggatcga cggttctcat tccgtagccg
      721 gcagctcagg cgaacagccg cttgacgccg acctcggggc tgacgatgcc gtcgtggatc
      781 atcgtgcctg cgacccacag gatgacggcg aggccgatcc aggcgatcca gcggtagcgc
      841 tcgatgtagc gggcgatgac attggcggcg acgcccatca gggcgaccga caggacgagg
      901 ccgacgatca agacccaggg atgctcgcgg gcggcgccgg cgaccgccag cacgttgtcg
      961 aggctcatcg agacgtcggc gagcgccacc gcccaggcgg ccgcggcgaa gctcctggcg
     1021 ggccgaaggc cggtgctctc gtccccctcg atttcctgcg acccgggcga ttggccggca
     1081 tggcgcagct cgcggtacat cttccaggcc acccacagca gcagcagccc gccggcaagg
     1141 acgaggccga tcagctggag gagctgggtg acgatcaggg cgaagccgat tcgaagcacc
     1201 aaggcggcgg cgatcccgat catgatcacc cggcggcgct gttcggccgg caggcccgcc
     1261 gccagcgcgc cgaccacgat cgcattgtcg ccggcgagga cgaggtcgat cagcacgacc
     1321 tgaaggaagg ccgcggccgg gccggttgtc gggaggctgc cgagatccat cggcgcccct
     1381 tagcggatca gcggcgcgag tccctagcgg gactattggt tcatggaggc gaagaagtcc
     1441 tcgttcgact tggcgtcctt catcttgtcg agcaggaact gcatcgcgtc caccgtgccc
     1501 atctgcatga ggatccgcct gagcacccac atcttcgaga gcgtgccctg gtcgaccagc
     1561 aattcctcct tgcgggtgcc ggacttgccg acgtcgagcg acgggaagat gcgcttgtcg
     1621 gagaccttgc ggtcgaggac gatctcggaa ttgccggtgc ccttgaactc ctcgaagatg
     1681 acctcgtcca tcttcgagcc ggtgtcgatc agggcggtgg cgatgatcga cagggatccg
     1741 ccttcctcga tgttgcgggc ggcgccgaag aagcgcttgg gccgctgcag ggcgttggcg
     1801 tcgacgccgc cggtcagaac cttgcccgac gagggcacga ccgtgttgta ggcgcgcccg
     1861 agccgggtga tcgaatcgag gaggatgacg acgtccttct tgtgctcgac gagacgcttt
     1921 gccttctcga tcaccatctc ggcgacctgg acgtgcctcg aagccggctc gtcgaaggtc
     1981 gaggagatga cctcgccctt caccgagcgc tgcatgtcgg tgacctcctc gggtcgctcg
     2041 tcgatgagca gcacgatcag gaagacctcg ggatggttgt cggtgatcgc ccgggcgatg
     2101 ttctggagga gcaccgtctt gccggtgcgc ggcggcgcga cgatcagggc gcgctggccc
     2161 ttgccctgcg gcgagatgat gtcgatcacc cgcgccgact tgtccttgat cgtcgggtcg
     2221 gcgctgtcga gggtgagctt ctcgtccggg tacagcgggg tgaggttgtc gaaattgacc
     2281 cggtggcgga cggcctccgg gtcgtcgtaa ttgacctggg cgagtcggac gagggcgaaa
     2341 tagcgctcgc cgtccttggg cccgcggatc tccccctcga ccgtgtcgcc ggtcctcagc
     2401 ccgaacttgc ggacctggtt gggcgagacg tagatgtcgt ccggaccggc gaggtaattg
     2461 gcctcgggcg agcgcaggaa gccgaagccg tcggggagga cctcgatcgt tccctggccc
     2521 atgatctcct ggccgttctc ggcctggacc ttgaggatcg agaacatcag ctcctgcttg
     2581 cgcagcgtcg acgcgccctc gatgccgagc tcctcggcca tggcgacgag gtcggcgggg
     2641 gatttttgct tcaaatcttt gagatgcatt gggggtactc agaaatagga tggtcggcag
     2701 gccgcgctgg gaaggatttt cagcggctgg aaatgcctac gcggacggca ggcgaacgac
     2761 ttcacaggcg ttaggctccg cgatttttca agtcaatcct tccttcgata cgccggttcg
     2821 acaggctcac cggctactca ggatgagcgg gcgggaaata tcctcaaccg ctcatcctga
     2881 gtagggactg agcttggcga aggcccgcat cgaaggacct gaagtcagaa cggcctcacg
     2941 atcaccagga cgacgatcag aaccaccgcc agccccggca cctcgttcat catccgaagg
     3001 ccttttccgc tgaggcggcg ttcgcccctg gcgagccggc ggccgtagcc gaccatccag
     3061 ccgtgatagg ccgagagggc gacgacgagg acgaacttgg cctggaacca gccgaacacc
     3121 caggcctcgt tgatccaggc cagggcgagg ccgaacatcc agaccaggat catcgccggg
     3181 gtgatgatga tccgcctcag cttgcgctcc cgctcgatcc agcgcgcttc ctcggccgat
     3241 cccggcgccg actcctggtg gtagacgtaa aagcgcggca gcatgaacag ccccgcgatc
     3301 cagaagatca cgaagatgac gtggccggcc ttgatccagg catagctcaa ggtgagaacg
     3361 gtcccccagt catagatcac actgtctctc cgccgtcttg gcgcaccagc gcgagaagct
     3421 gt
//
LOCUS       DASVQZ010000005        13789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_673994, whole genome shotgun sequence.
ACCESSION   DASVQZ010000005 DASVQZ010000000
VERSION     DASVQZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673994"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VF547_00160"
     CDS             complement(<1..1539)
                     /locus_tag="VF547_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8261266.1"
                     /translation="MRPSFHPIQCSIIALAAALAAPALAQAPAAQPGRPAQAPSRPAG
                     ERPQEVVVTGTRAEVVATPDRTSFNVANDLQVQNGTLADALRAVPGVEVDLQGNVSLR
                     GDPGVTIHIDGRPSAMLKGDNRGDVILSMPAGRIERVEVMTNPSAANSPEGSAGIINL
                     VTRQARKDMTTGTVRATAGLKGRGAIGLSGTHNAGDLTLTGELGYRRFTGETEAVQLR
                     SRLDPATGGFVDSRLDSEIDQRNAFANARAGIEYDLDKRNRLSAELGYRNGRGEVERV
                     DSFAAADPAFSYERAAEIDLRQRGPSAHASWRRTLPGKDHELSADLEVERVRMNRDVE
                     AATDFAGAPDFVERIRNSIRRSDENLKLDYKRRVGEESTLNLGYQGSFTRSEFDFSGE
                     RGPGFGALVPVPGLTNSFDYDQAIHALFGTFVFPVGKLDAQAGLRLEQAETELDQVTD
                     AVSFENDYFRIYPTLHLGYELGKREQLKASYSRRIQRPSPQDLNPYTFYIDPQNLRRG
                     NPALR"
     gene            complement(1651..2526)
                     /locus_tag="VF547_00165"
     CDS             complement(1651..2526)
                     /locus_tag="VF547_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261267.1"
                     /translation="MNSPEPARHSFGMLLRSQLRATGLAIHREAGLAAAALALAGLLV
                     VTSALRYHEELNALPEILLATLPIALLLPWAVWRGDPVFGRAYLWTLPVRRQRAAAAK
                     IAAGALWLVAILLVAMASIFAMAAITGGTFGTEETRLVGPFGSGPAAAVRVPWTTPLW
                     MWLAPFGGALTLYVASSAALVGLRHPLRWLGGVAVAVTLLMVIALNMESHNPLHQGLG
                     RLAEALVGGRWGLDFALTGGAAALSEEIDLPGPGSVTMWRALPTAGAWAAALAAWLGA
                     ALLALALALRRHWER"
     gene            complement(2513..3439)
                     /locus_tag="VF547_00170"
     CDS             complement(2513..3439)
                     /locus_tag="VF547_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8261268.1"
                     /translation="MDNNGAPALAVRTDGLAKSFGRERALDGVHLAVPEGGVYLLAGT
                     NGAGKSTLLRTLLNVERPDSGAAFVLGLDTSLNGAEVRARTGYVPETGEGTYRWMRAG
                     RFLAQHSAFYPAWDEDYARHLVRILEVREDARLGALSKGQARRVQIVAALAHRPPLLL
                     LDELTDGLDPLAREEVLALLAEHLADTKATAILSTHLVAEVETLIDHVGVLRSGRLVA
                     QAPAERLLGQLRRYHLESADGWRAPPALEARVVRRESGLGRSVRLVAAGAEPDIAAAV
                     AASGARVREVDRLSLAEAVPILLQSESSHEFA"
     gene            complement(3432..3815)
                     /locus_tag="VF547_00175"
     CDS             complement(3432..3815)
                     /locus_tag="VF547_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8261269.1"
                     /translation="MQLSIDTRAAKPIYVQVMDEVRRLVAVGEIAAHDMLPSVRQLAA
                     ELRLNHNTIVQAYRELEREGVVYARRGQGTFVAEPSGTEAERDRLLADVVERALTDAH
                     RHGIAPLALIDALSKAAGTQGGKNG"
     gene            4162..4785
                     /locus_tag="VF547_00180"
     CDS             4162..4785
                     /locus_tag="VF547_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261270.1"
                     /translation="MNTRRIAQSYYGIKLLYEFGPEELVHTRTDLSTTHRCSARYENI
                     GVGNPSYLALENRLFTVTALLTAISLAVAALTMIVAYRLQPWLVSIPLGVVPLGFLLV
                     RMRTPFSLHFVLLPVWLDAFGGEAPPIWLRDDDRGQEVLDELVLRWRERLRSLYASVD
                     PDNDAEREAAKFGWLRENGIIEEAEYQAALGEIELLCAQSGSERPLH"
     gene            complement(5165..6802)
                     /locus_tag="VF547_00185"
     CDS             complement(5165..6802)
                     /locus_tag="VF547_00185"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8261271.1"
                     /translation="MARFIFITGGVVSSLGKGLMAAALAALLQARGYKVRIRKLDPYL
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVPARQADNVTSGRIYQGIIQRE
                     RRGDYLGATVQVVPHVTNAIKEFIAADLEADLDFLICEIGGTVGDIESLPFIEAIRQF
                     RNERPRGQSLSIHTTLVPYIAAAGELKTKPTQHSVRELTSLGIQPEILVCRAEHPLPD
                     GDRAKIALFCNVRKEAVIPALDAKSIYAVPAQYHEAGLDAEVLRAFGIEDAPEPRMQR
                     WDDVIDRLFNWESEVTIGVVGKYVGLPDAYKSLTEALVHGGLANRVKVRIAWLDAELF
                     EGPEADIPAQLEPLHGILVPGGFGERGSEGKIASVHFARERKVPFFGICLGMQMACIE
                     AARNLAGLPGAASTEFGEAEEPVVGLITEWMSKEGLQRREAEGELGGTMRLGAYEAVL
                     AGNSHVRSIYGAERISERHRHRYEVNIHYKTALEQEGLLFTGMSPDGALPEIVERPDH
                     PWFVGVQFHPELKSKPFDPHPLFASFIEAAVKQSRLV"
     gene            6979..8232
                     /locus_tag="VF547_00190"
     CDS             6979..8232
                     /locus_tag="VF547_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX8261272.1"
                     /translation="MRVAIVGAGYSGTVAAVEIARAAPGAGIVLVEKSGRFAAGAAYG
                     TDSPGHLLNVRARNMSALAGDPDHFAAWAAGRGHGPDDYVPRRDFRRYLAALLDATPG
                     VSRVTGEAAAVEDGGLRLADGTRIGCDVVVLAGGNFPSRLPERLGPAAIHDPWSLDGA
                     AAVAKAAAGGGELLLVGTGLTMVDMAVSLEEAGFPGRITAASRRGLVPRPHVSPPVPP
                     LDWDPPGRLGALVRELRCHPEWRAAVDGLRPHSIGLWQGLGEAEKKRFLRHLRPWWDV
                     HRHRIAPQVAARIQAMRDSGRLDVAAGRIARVEDGEVAIARRGGGELRRRFTAAINCT
                     GPEGDIRRVADPLIRRLLATGRARADRLGLGLEVDRRSRVVGNGPSPALYALGPLTKG
                     AFWEIVAVPDIRGQAAAVARDIAAD"
     gene            complement(8365..8751)
                     /gene="secG"
                     /locus_tag="VF547_00195"
     CDS             complement(8365..8751)
                     /gene="secG"
                     /locus_tag="VF547_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX8261273.1"
                     /translation="MFTFLLILQSLVAASMVTVILMQRSEGGGLGVGGSPSGLMSARG
                     AADFLTRATSILAVLFVVLSIALAGLATIERAPTKIDPTLSKSAPQGTPLAPAAIPPA
                     ADPLAGAAAQAPANSAAGNGAVPLAQ"
     gene            complement(8896..9645)
                     /gene="tpiA"
                     /locus_tag="VF547_00200"
     CDS             complement(8896..9645)
                     /gene="tpiA"
                     /locus_tag="VF547_00200"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX8261274.1"
                     /translation="MHLRRKLVAGNWKMNGSLAALAELAAIAQAARNAGGVDVAVCPP
                     FTLIAAASARSGGLAVGGQDCHWAASGAHTGAVSAAMLKEAGARLVIVGHSERRAEAG
                     ETDGVVRAKAEAALAAGLQAIVCVGESEEERAAGRAVERVSGQLAGSLPDPGGDGGLI
                     VAYEPIWAIGTGKVASPGDVAEMHAAIRRSLVERFGDSGGRIRILYGGSVSAANAASL
                     FAVDDVDGALVGGASLTAAQFVPIVEAAARS"
     gene            9800..11746
                     /locus_tag="VF547_00205"
     CDS             9800..11746
                     /locus_tag="VF547_00205"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8261275.1"
                     /translation="MLSLFRRGFMAKIMLVVLFISLIAIVITGFGTGGGGLGDLGGLR
                     SGTVASVGGEKITSDRLRSETQRQFERIRREQPELDMAAFLRRGALEEVLDQLIDMAA
                     IAVFGREHGLGVSRQMIDRDIAGVPAFQDVAGRFDDATFRRVLAQEGLTEQQLRDDIE
                     GRLIQRQLVLPVAGSAYVPNAIAYQYASLLLETRSGIVGAVPSAAMGPAQEPTEPELA
                     AFYRTNQARYTIPERRVLRYALFGPEQVAAQSQATEAEIQAAYRQNPAYAARETRSLS
                     QVVLQNEAAARALAQKIAGGTSFAAAAAQAGYGPSDTALGANSREAFAAKSSPAVAAS
                     VFAAAEGSTVGPVRSPFGWHVVRVDDVIVSAARPLAAVRAEIAAQVQQRKATNALSDL
                     AARIENAIGDGSSFDEIVAAQKLAVQETVPVTATGAAPDNPGWQAPAELKALLEGAFA
                     MEPGEDPAVETIQENQRYALLAVSRSIPAAAPPLAQIRDRVKADLLVRRASERARAVA
                     TAIVARINAGTPPSQAFAQAPVKLPAVQTLTASRRDIARQNAQVPPPLAMMFSLPRGK
                     ARILPAPDGRGWFIVYLDRIVPGDARKEPGLAEAVRGQFAQILGDEYARQFTRAIRAG
                     LKIKRNDGAVRALRADLSGTGTAQ"
     gene            11756..12355
                     /locus_tag="VF547_00210"
     CDS             11756..12355
                     /locus_tag="VF547_00210"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX8261276.1"
                     /translation="MGPVSILVLDNYDSFTWNIVHYLREVGAEVRVERNDSLTAAEAL
                     ASGARAFLISPGPGRPDGAGESLALVAACAEARRPLLGICLGHQAIAQHFGGRIVRAE
                     SVMHGKTCGVRHDSTGLFEALPSPLTAARYHSLAVDPSSVPACLAVNATADDESVQGL
                     RHRTLPIHGVQFHPESVASDKGHELLANFVRIAACSGTA"
     gene            12410..13063
                     /gene="lexA"
                     /locus_tag="VF547_00215"
     CDS             12410..13063
                     /gene="lexA"
                     /locus_tag="VF547_00215"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX8261277.1"
                     /translation="MLTRKQSELLTYIQAQLAESGISPSFEEMKEALALKSKSGVHRL
                     ISALEERGFIRRLPNRARALEVLRMPDAQSGIAAQPKPPAAAPQAANDVIELPLHGRI
                     AAGMPIEALEGQTSLPVPAALLGPGEHYALEVAGDSMVEEGILDGDYALIRKTDVARD
                     GEIVVALIDDNEATLKTYRKEGQMIRLDPANRRYDPQRYRPEQVRIQGRLSGLLRRY"
     gene            13212..13550
                     /locus_tag="VF547_00220"
     CDS             13212..13550
                     /locus_tag="VF547_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8261278.1"
                     /translation="MARLNYVELPVADIGAAKRFYEAAFGWSLTGFGPTYAATMTGDT
                     DIGLQADSAEASKAPLPVIDVPDLEAALASVEAAGGRIVRPIFAFPGGRRFHFLDPGG
                     NELAAVTADP"
BASE COUNT         1991 a         4771 c         5028 g         1999 t
ORIGIN      
        1 cctcagcgcc ggattgcctc ggcgaaggtt ctgcgggtcg atgtagaagg tgtaggggtt
       61 gaggtcctgc ggcgagggcc gctggattcg ccggctgtag ctcgccttga gctgctcgcg
      121 cttgccgagc tcgtagccga gatggagcgt cggatagatt cggaaatagt cgttctcgaa
      181 gctcaccgcg tcggtgacct ggtcaagctc cgtctccgcc tgctcgagcc ggaggcccgc
      241 ctgggcgtcg agcttgccga ccgggaagac gaaggttccg aacagggcgt ggatcgcctg
      301 gtcgtagtcg aagctgttgg tgaggccggg aacgggcacg agcgctccga agcccgggcc
      361 gcgctcgccc gagaagtcga actcgctgcg cgtgaagctg ccctgatagc cgaggttcag
      421 ggtcgattcc tcgccgactc ggcgcttgta gtcgagcttg aggttctcgt cggagcggcg
      481 gatggagttg cggatccgct cgacgaagtc gggcgcgccg gcgaagtcgg tggccgcctc
      541 gacgtcgcgg ttcattcgga cccgctcgac ctcgaggtcg gcggacagtt catggtcctt
      601 gccgggcagg gtgcggcgcc aggaggcgtg cgcggagggc ccgcgctgcc tgaggtcgat
      661 ctcggcggcg cgctcgtagg agaaagcggg gtcggcggcg gcgaagctgt cgacgcgctc
      721 gacctcgccg cggccgttgc ggtagccgag ctcggcgctg aggcggttcc tcttgtcgag
      781 atcatattcg attccggcgc gggcattggc gaaggcgttg cgctggtcga tctcggagtc
      841 gagccggctg tcgacgaacc cgccggtcgc cgggtcgagt cgggagcgaa gctggacggc
      901 ctcggtctcg ccggtgaagc ggcggtagcc gagctcgccg gtgagggtga ggtcgccggc
      961 attatgggtt ccgctgaggc cgatcgcgcc gcgtcccttg aggccggcgg tcgcccggac
     1021 ggtgccggtg gtcatgtcct tgcgggcctg gcgggtgacg aggttgatga tcccggccga
     1081 gccttcgggg ctgttcgcgg ccgacgggtt ggtcatcacc tcgacccgct cgatccgccc
     1141 ggccggcatc gacaagatga cgtcgccgcg attgtcgccc ttgagcatcg ccgagggccg
     1201 gccgtcgatg tggatggtga ctccgggatc gccgcggagg ctgacattgc cctggaggtc
     1261 gacctcgacc ccggggacgg ctcggagcgc gtcggcgagg gtcccgttct gcacctggag
     1321 gtcgttggcg acgttgaagc tcgtccggtc gggagtggcg acgacctcgg ctcgggtgcc
     1381 ggtgacgacc acctcctgcg gccgctcgcc ggccggtcgg gacggagcct gggcggggcg
     1441 gcccggctgg gcggccgggg cctgggcgag ggccggtgcg gcgagggcgg cggcaagggc
     1501 gatgatcgag cactggatcg ggtggaagct gggtcgcatg atgcattggc ctttgaagaa
     1561 gatgagcgcg cttctgcctg gcccagtcgc gggcaggcgg gatgagggat cggaggagtt
     1621 tcgttgcagc ccgcaatcgg cgggcccggg tcagcgctcc cagtgcctcc ggagcgcgag
     1681 ggccagcgcc agcagcgccg ctccgagcca ggccgcaagc gccgccgccc aggcgccggc
     1741 cgtgggcagg gcgcgccaca tggtgaccga gcccgggccg ggaaggtcga tctcctcgga
     1801 caaggccgcg gcgccgccgg tgagggcgaa atcgagtccc cagcggccgc cgaccagggc
     1861 ctccgccaag cggccgagcc cctggtggag cggattgtgg gattccatgt tgagcgcgat
     1921 caccatcagc agcgtcaccg cgaccgcgac tccgccgagc cagcgcaacg gatggcggag
     1981 cccgacgagt gcggcgctgc tcgccacata gagggtcaag gcgccgccga acggcgccag
     2041 ccacatccag aggggcgtgg tccaggggac ccgcaccgcg gccgccggcc cggagccgaa
     2101 aggcccgacc agacgcgtct cctcggtccc gaaggtgccg ccggtgatgg ccgccatggc
     2161 gaagatcgag gccatggcga cgagcaggat cgccaccagc cacagggcgc ccgcggcgat
     2221 cttggcggcg gccgcccgct ggcggcggac gggcagggtc cacagatagg cccggccgaa
     2281 aaccggatcg ccccgccaga ccgcccaggg aagcagcagc gcgatcggaa gggtcgccag
     2341 caatatctcc ggcaatgcgt tcagctcctc atggtagcgg agcgcgctcg tcacgacgag
     2401 caggccggcc agggccaggg ccgccgcggc cagccccgct tcgcgatgga tggcgaggcc
     2461 ggtcgcgcgc agctggctgc gcagcagcat cccgaacgag tggcgcgccg gttcaggcga
     2521 attcatggct tgactccgac tgaaggagga tcgggacggc ttcggccagg ctcagccgat
     2581 cgacctcccg cacccgcgcg cccgaagcgg cgacggcggc ggcgatgtcg ggctcggcgc
     2641 cggcggcgac cagccgcacc gacctgccga ggcccgactc gcgccggacc acccgcgcct
     2701 cgagcgccgg cggcgcccgc cagccgtccg ccgactcgag atggtagcgg cggagctgac
     2761 ccagcagccg ttccgccggg gcctgggcga ccagcctgcc cgagcggagc accccgacat
     2821 ggtcgatcaa ggtctcgacc tcggcgacga ggtgggtcga gaggatcgcg gtcgccttgg
     2881 tgtcggcgag atgctcggcg agcagcgcga gcacctcctc gcgggcgagc ggatcgagac
     2941 cgtcggtgag ctcgtcgagg agcaggagcg gcggccggtg ggcgagcgcg gcgacgatct
     3001 ggactcgccg cgcctgcccc ttggagagcg cgccgaggcg ggcgtcctcg cggacctcga
     3061 ggatccggac gaggtggcgc gcataatcct cgtcccaggc cgggtagaag gccgaatgct
     3121 gcgccaggaa gcgtcccgcc cgcatccagc gataggtccc ctcccccgtc tccggcacat
     3181 agccggtcct ggcacgcacc tcggcgccgt tgaggctcgt gtcgaggccg agcacgaagg
     3241 cggccccgct gtccggccgc tcgacgttca agagggtgcg aagcagcgtg ctcttgccgg
     3301 ccccgttggt cccggcgagc agatagaccc ccccttccgg aacggccaga tggacgccgt
     3361 ccagcgcgcg ctcgcggccg aagctcttgg cgagcccgtc ggtccgcacc gccaaggccg
     3421 gggccccgtt attatccatt cttgcctcct tgggtgccgg cggccttgct cagagcgtcg
     3481 atcaaggcga gcggagcgat gccgtggcgg tgggcgtcgg tcagcgcccg ctcgacgacg
     3541 tcggcgagca gccggtcgcg ctccgcctcc gttccggagg gctcggcgac gaacgtcccc
     3601 tgcccccgcc gcgcatagac cacgccctcg cgctcgagct cgcgataggc ctggacgatc
     3661 gtattgtggt tgagcctgag ctcggcggcg agctgccgca ccgacggcag catgtcgtgg
     3721 gccgcgatct ccccgaccgc gaccagccgg cgcacctcgt ccatcacctg gacgtagatg
     3781 ggcttggccg cccgggtgtc gatcgaaagc tgcaagaacc cctccgttgt ccggtgtact
     3841 aggacactgg tacaattgtg tcaaggctgt tccgcccccc gcggaaaagc ggcttcatgg
     3901 ctcacgctcc ccggatgcac ggagagaaaa gaagagggct tcgacaagct cagcccgaac
     3961 ggagatggcg cgatccgact cccggccccc caggggattt gctcgaatcg ctctcaccgt
     4021 tcgccctgag cctgtcgaag ggctttcctt gtcgttcgat aaaagaatag gacacggcct
     4081 cacgagctcg gccgaacgca agaaagcgca tccgactgcc gagcggttta ggggcgccgg
     4141 agaaagcccg ggaaggtcca agtgaacacc cgccggatcg cgcagtccta ctacggcatc
     4201 aagctgctct acgagttcgg cccggaggag ctggtccata ccaggaccga cctgtcgacg
     4261 actcaccgct gctccgcgcg ctacgagaac atcggagtgg gcaatccctc ctaccttgcg
     4321 ctcgagaacc gtctgttcac ggtcacggcc ctgctgactg cgatctcgct agccgtagcg
     4381 gccctgacga tgatcgtcgc ttaccgtctg cagccctggc tggtgagcat ccccttgggg
     4441 gtggttccgc tcggcttcct gctggttcgg atgaggactc ccttctcgct gcatttcgtc
     4501 cttcttccgg tctggctcga cgcgttcggc ggggaggcgc cgccgatctg gttgcgggac
     4561 gacgaccggg gacaggaggt gctggacgag ctcgtgctga gatggcgcga gcggcttcga
     4621 agcctctacg cctccgtcga tccggacaat gacgcggaac gcgaagcggc gaagttcggc
     4681 tggctcaggg agaatgggat catcgaggag gcggaatatc aggccgccct cggcgagatc
     4741 gagctgctct gcgcccaatc gggatccgag cgcccgctcc actaggagac ggagggatca
     4801 ggtcgaatcg ctctcacccg ttcgccctga gcctgtcgaa gggctttcct tgtcgttcga
     4861 taaggaaaag gagacggcaa aattccgagg ccagcttcgc cgattgccgc gccgaggcgt
     4921 tcgtggtatg ttctagtcgg cgctcgccgt gcggaggctg cccggaagga ccggatcggc
     4981 cggtctggcg aaaaaggagc gtgggtgagg aatgcgggcg ttggccgata acgctgtccg
     5041 acccggccgc gcatgccgct gcgagagcgg aggtttgcgg gcgaagttca cgacaaaggc
     5101 gctcgactcg acgaacttca tgaacttcgc cggcaagcgc cgggccttgc aagaatttgc
     5161 aggctcagac gagccggctc tgcttcaccg cggcctcgat gaagctggcg aagagcgggt
     5221 ggggatcgaa gggcttggat ttgagctccg ggtggaactg cacgcccacg aaccagggat
     5281 ggtccggccg ctcgacgatc tccggcaggg cgccgtcggg gctcattccg gtgaagagga
     5341 ggccctcctg ctcgagggcg gtcttgtagt ggatgttgac ctcgtagcgg tggcggtggc
     5401 gctcgctgat gcgctcggcg ccgtagatgc ttcgcacgtg gctgttgccg gccaggaccg
     5461 cctcgtaggc gcccagccgc atggtgccgc cgagctcgcc ttcggcttcg cgcctctgga
     5521 ggccctcctt gctcatccat tcggtgatga ggccgacgac cggctcctcg gcttcgccga
     5581 attcggtcga ggcggcgccc gggaggccgg cgaggttcct cgccgcctcg atgcaggcca
     5641 tctgcatgcc gaggcagatg ccgaagaagg ggaccttgcg ctccctggcg aaatggacgc
     5701 tggcgatctt gccctcggag ccgcgctcgc cgaagccgcc ggggacgagg atgccgtgaa
     5761 gcggctccaa ttgggcgggg atgtcggctt ccgggccctc gaacagctcg gcgtcgagcc
     5821 aggcgatgcg gaccttgacg cggttggcga ggccgccgtg gaccagcgcc tcggtcagcg
     5881 acttgtaggc gtcggggagg ccgacatatt tgccgacgac cccgatcgtg acctcgctct
     5941 cccagttgaa caggcggtcg atgacgtcgt cccagcgctg catgcgcggc tccggggcgt
     6001 cctcgatgcc gaaggcgcgg agcacctcgg cgtcgagccc cgcctcgtga tactgggcgg
     6061 ggacggcgta gatgctcttg gcgtcgaggg cggggatgac cgcctccttc ctgacgttgc
     6121 agaacagggc gatcttggcg cggtcgccgt ccggcagcgg gtgctcggcg cggcagacga
     6181 ggatctcggg ctggattccg aggctggtca gctcgcggac cgaatgctgg gtcggcttgg
     6241 tcttcagctc gccggcggcg gcgatgtagg ggacgagggt ggtgtggatc gagagcgact
     6301 ggccgcgggg acgctcgttc ctgaactggc ggatcgcctc gatgaacggc aggctctcga
     6361 tgtcgccgac ggtgccgccg atctcgcaga tgaggaagtc gagatccgcc tccaggtcgg
     6421 cggcgatgaa ctccttgatc gcgttggtga cgtgggggac gacctggacg gtggcgccga
     6481 gatagtcgcc gcgccgctcg cgctggatga tgccctggta gatccggccc gaggtgacgt
     6541 tgtcggcctg gcgcgcgggg accccggtga agcgctcgta atggccgagg tcgagatcgg
     6601 tctcggcgcc gtcgtcggtg acgtagactt cgccatgctg gtagggcgac atcgtgcccg
     6661 gatcgacgtt gagatagggg tcgagcttgc ggatcctcac cttgtagccg cgcgcctgga
     6721 ggagggcggc gagggcggcg gccatcaggc ccttgccaag cgaggagacc acgccgccgg
     6781 tgatgaaaat gaaccgcgcc atgggaatca ccggatagag aaggcgggcg cggatgggca
     6841 agggcgcgaa tcggaaatat tcgtcgtccc ggcgcagccg ggacccccga cgggtgaaaa
     6901 gatgcggtgc cttccactcc gcgggacccc ggccttggct ggggtgacgg cggctcggcc
     6961 agaggctaag ccgccgtcat gcgggtcgcg atcgtcgggg cgggttattc ggggacggtg
     7021 gcggcggtcg agatcgcgcg cgccgctccg ggcgccggaa tcgtcctcgt cgagaagagc
     7081 gggcggttcg ccgcgggggc ggcctacggc accgattcgc cgggccatct cctcaacgtg
     7141 cgcgcccgca acatgagcgc cctcgccggc gatccggacc atttcgcggc ctgggccgcc
     7201 ggccggggcc acgggcccga cgattacgtg ccgaggcgcg acttccggcg ctacctcgcg
     7261 gccctgctcg acgcgacgcc gggggtgagc cgggtgacgg gcgaggcggc ggcggtcgag
     7321 gatggcgggc tccggctcgc cgacgggacc cggatcggct gcgacgtcgt cgtcctcgcc
     7381 ggcggcaact tcccgtcgcg cctgcccgag cggctcggcc cggcggcgat ccacgaccct
     7441 tggagcctgg acggagcggc ggcggtggcg aaggccgcgg cggggggcgg cgagctgctg
     7501 ctggtcggga ccggcctgac catggtcgac atggcggtca gcctcgagga agccggcttt
     7561 cccggtcgga tcaccgccgc ctcgaggcgc ggcctggtgc cgcggccgca cgtctcgccg
     7621 ccggtgccgc cgctcgactg ggacccgccg gggcggcttg gggcgctggt gcgggagctg
     7681 cgctgccatc cggaatggcg ggcggcggtc gacgggctcc ggccgcacag catcggcttg
     7741 tggcagggcc tcggcgaggc ggagaagaag cgcttcctgc gccaccttcg cccctggtgg
     7801 gacgtgcacc ggcaccggat cgcgccccag gtggcggccc ggatccaggc gatgcgcgat
     7861 tcgggccggc tcgacgtggc ggcggggcgg atcgcgcgag tcgaggacgg cgaggtggcc
     7921 atcgcccgcc gcggcggcgg cgagcttcgg cgccgcttca ctgcggcgat caactgcaca
     7981 ggcccggaag gcgacatccg gcgggtcgcc gacccgctga tccgccggct gctcgcgacc
     8041 ggccgggcgc gggcggaccg gctcgggctc ggactcgagg tcgaccggcg ctcgcgggtg
     8101 gtcgggaacg gcccctcccc tgccctctac gcgctcggcc ctctgaccaa gggcgcgttc
     8161 tgggagatcg tcgcggtgcc cgacatacgc gggcaggcgg cggcggtggc gagggatatc
     8221 gccgccgact gagcggtcct tcgatacggg ccttcgccag gctcagtccc tactccggat
     8281 gagcgattga ggatcaccct gccgctcatc ctgagtagcc ggtgagcttg tcgaaccggc
     8341 gtatcgaagg acgatcggaa gcgtttattg cgcgagcggc accgcgccgt tgccggcggc
     8401 cgagttggcc ggcgcctggg cggcggcgcc cgcgagcggg tcggcggcgg gaggaatcgc
     8461 ggccggagcg agcggcgttc cctgcggggc cgatttggac agggtcggat cgatcttggt
     8521 cggcgcccgc tcgatcgtgg cgaggccggc gagcgcgatc gagaggacga cgaagaggac
     8581 cgcgaggatc gaggtggcgc gggtcaggaa gtcggccgcg ccgcgcgccg acatcaggcc
     8641 cgaggggctg ccgccgacgc cgagcccgcc gccctccgag cgctgcatca ggatcacggt
     8701 caccatcgag gcggcgacca gcgactggag gatgaggagg aaggtgaaca ttgaacggcc
     8761 tcgggtctgg agttcgccac cccagcggag gctggggtcc cagggcgtga aaaatgcgac
     8821 gttcttcacc cgctcgggtc ccggcctgcg ctgggacgac gcgtggcgcg gacttaggcg
     8881 atcgcgcggc cggcttcaac tcctcgccgc cgcctcgacg atcgggacga actgggccgc
     8941 ggtgaggctg gcgccgccga cgagcgcgcc gtcgacgtcg tcgaccgcga acagcgaggc
     9001 ggcattggcg gcgctgaccg agccgccgta gaggatccgg atccggccgc cggagtcgcc
     9061 gaagcgctcg accagcgagc ggcggatggc ggcgtgcatc tcggcgacgt cgcccgggct
     9121 cgccaccttg ccggtgccga tcgcccagat cggctcgtag gcgacgatca ggccgccgtc
     9181 cccgcccgga tcggggagag agccggcgag ctggccgctg acccgctcga ccgcccggcc
     9241 cgccgcccgc tcctcctcgc tctcgccgac gcagacgatc gcctgcaggc cggcggcgag
     9301 cgccgcttcg gccttggcgc ggacgacgcc gtcggtctcg ccggcctcgg ctcggcgctc
     9361 gctgtggccg acgatgacaa gcctggcgcc ggcctccttc agcatcgccg cggagaccgc
     9421 gccggtatgg gcgccgctcg ccgcccaatg gcagtcctgg ccgccgaccg cgagcccccc
     9481 ggagcgggcg gaagcggccg cgatcagggt gaagggcggg cagacggcga cgtccacccc
     9541 gcccgcgttc cgagccgcct gggcgatcgc cgcgagctcg gcgagcgcgg cgaggctgcc
     9601 gttcatcttc cagttgccgg cgacgagctt gcggcggagg tgcatgtcca attccctgcg
     9661 tggagagggc cgaggcgctg gctcgacggg aacccctgcc gcgggcgcct agcaaccgcg
     9721 ggggcgagtg ccaagcttgc ccgcgccggg ccgcccttct atgacccgcg cgaactcccc
     9781 atcgcaggat caacgcccga tgctctcctt gttccgccgc ggcttcatgg cgaagatcat
     9841 gctggtcgtc ctcttcatca gcctgatcgc gatcgtcatc accggcttcg gcaccggcgg
     9901 cggcggcctc ggcgacctcg gcgggctgcg cagcggcacc gtcgcaagcg tcggcggcga
     9961 gaagatcacc tccgaccggc tgcgcagcga gacccagcgc cagttcgagc gcatccgccg
    10021 cgagcagccc gagctcgaca tggcggcctt ccttcgccgc ggcgcgctcg aggaggtgct
    10081 cgaccagctg atcgacatgg ccgcgatcgc cgtcttcggc cgcgagcacg gcctcggcgt
    10141 ctcgcggcag atgatcgacc gcgacatcgc cggcgtcccc gccttccagg acgtggcggg
    10201 gcggttcgac gacgccacct tccgccgggt cctcgcccag gaggggctga ccgagcagca
    10261 gctgcgcgac gacatcgagg gccggctgat ccagcgccag ctcgtgcttc cggtcgcggg
    10321 ctcggcctat gtgccgaacg ccatcgccta ccaatatgcc tcgctgctgc tcgaaacccg
    10381 cagcggcatc gtcggagcgg tgccgtccgc ggcgatgggc ccggcgcagg agccgaccga
    10441 gcccgagctc gccgccttct accggaccaa ccaggcccgc tacacgatcc ccgagcggcg
    10501 ggtgctgcgc tacgccttgt tcggccccga gcaggtcgcc gcccagtccc aggccaccga
    10561 ggccgagatc caggccgcct accgccagaa ccccgcctac gcggcgcgcg agacccgcag
    10621 cctgtcgcag gtggtgcttc agaacgaggc cgcggcgcgc gctctggccc agaagatcgc
    10681 cggcggcacc agcttcgccg ccgccgccgc ccaggccggc tacggcccct ccgacaccgc
    10741 gctcggagcc aacagccgcg aggccttcgc cgcgaagagc tcgcccgccg tcgccgcctc
    10801 ggtgttcgcc gcggccgagg gctcgacggt ggggccggtc cgctcgccgt tcggctggca
    10861 cgtcgtccga gtggacgacg tcatcgtctc ggcggcgagg ccgctcgccg cggtccgagc
    10921 cgagatcgcg gcgcaggtcc agcagcgcaa ggcgaccaac gccctcagcg acctcgccgc
    10981 ccggatcgag aacgcgatcg gcgacggctc gagcttcgac gagatcgtcg cggcgcagaa
    11041 gctggccgtc caggagacgg tccccgtcac cgccaccgga gccgcccccg acaatcccgg
    11101 ctggcaggcg ccggccgagc tcaaggcgct gctcgaaggc gcgttcgcga tggagccggg
    11161 cgaggatccg gcggtcgaga cgatccagga gaatcagcgt tacgcgctgc tcgccgtcag
    11221 ccgctcgatc ccggccgcgg cgccgccgct ggcccagatc cgcgaccggg tgaaggccga
    11281 ccttctggtc cgccgcgcct cggagcgcgc gcgcgcggtg gcgaccgcga tcgtcgcccg
    11341 gatcaacgcc gggactccgc cgtcccaggc cttcgcgcag gcgccggtca agctgcccgc
    11401 cgtccagacg ctcaccgcca gccggcgcga catcgcccgc cagaacgcgc aggtgccgcc
    11461 gccgctggcg atgatgttct cgctgccgcg cggcaaggcc cggatcctgc cggcgccgga
    11521 cgggcggggc tggttcatcg tctatctcga ccggatcgtg cccggcgacg cccgcaagga
    11581 gcccggcctc gccgaagcgg tgcgcgggca gttcgcccag atcctcggcg acgaatatgc
    11641 ccggcaattc acccgggcga tccgcgcagg cctgaagatc aagcgcaacg acggggcggt
    11701 ccgcgcgctc agggccgacc tctccggcac cggaaccgcc caatagggct cgagcttggg
    11761 acccgtctcg atcctcgtcc tcgacaatta cgacagcttc acctggaaca tcgtccacta
    11821 tctgcgcgag gtgggcgccg aggtgcgggt cgagcgcaac gattccctca ccgccgcgga
    11881 ggcgctggcg agcggcgccc gggccttcct gatctcgccg ggcccggggc gcccggacgg
    11941 cgccggcgag tcgctggcgc tggtcgccgc ctgcgccgag gcgcgtcggc ccctgctcgg
    12001 catctgcctc ggccaccagg cgatcgccca gcatttcggc gggcgcatcg tgcgcgccga
    12061 gtcggtcatg cacggcaaga cctgcggcgt gcgccacgat tcgaccggac tgttcgaggc
    12121 ccttccctcg cccctgaccg cggcccgcta ccactcgctg gccgtcgacc cgtcgagcgt
    12181 tcccgcctgc ctcgcggtca acgccaccgc ggacgacgaa tcggtccagg gcctgcgcca
    12241 ccggaccctg ccgatccacg gcgtgcagtt ccatcccgag agcgtcgcca gcgacaaagg
    12301 ccatgaattg ctggcgaatt ttgtcagaat cgcggcttgt tccggaaccg cttgacacgg
    12361 cctgtaacgt tccctacacg ttccgcatac gtaccaaggg gatcgtgaaa tgctgacccg
    12421 caagcaaagt gagcttctca cctacatcca ggcgcagctc gccgagagcg gcatctcgcc
    12481 ttcgttcgag gagatgaagg aggcgctggc gctcaagtcc aagtcggggg tgcatcggct
    12541 gatctccgcg ctcgaggaac gcggcttcat ccgccggctg cccaaccgcg cccgggcgct
    12601 cgaggtcctg cgcatgcccg acgcccagtc cggcatcgcc gcgcagccca agccgcccgc
    12661 ggcggcgcct caggccgcca acgacgtcat cgagctgccg ctccacggcc ggatcgccgc
    12721 cggcatgccg atcgaggcgc tggaggggca gaccagcctc cccgtccccg ccgccctgct
    12781 cggcccgggc gagcattacg cgctcgaggt ggccggcgac tcgatggtcg aggaaggcat
    12841 attggacggc gactatgcgc tgatccgcaa gaccgacgtc gcccgcgacg gcgagatcgt
    12901 ggtcgctctg atcgacgaca acgaggcgac cctcaagacc taccgcaagg aaggccagat
    12961 gatccgtctc gacccggcca accgccgcta cgatccgcag cgctaccggc ctgagcaggt
    13021 ccgcatccag ggccgtctgt ccggcctgct tcggcggtac tgagccttcg atccctctcc
    13081 ctgcaggggt gagggacggc cgcgaagcgg cagggcgagg ggccggctcc ggttctgccc
    13141 ccaccctccc gccgctcgcg gcgggccccc tacctctccc ctgcagggag aggggaagaa
    13201 aggagcatcg catggcccgt ctcaactatg tcgagctgcc ggtcgccgat atcggggccg
    13261 cgaagcgctt ctacgaggcc gcgttcggct ggagcctgac cggcttcggg ccgacctatg
    13321 cggcgacgat gaccggcgac accgacatcg gcctgcaggc cgactccgcc gaggccagca
    13381 aggcgccgct gccggtgatc gacgtccccg acctcgaagc cgcgctggcg tcggtcgagg
    13441 cggccggcgg ccgcatcgtc aggccgatct tcgcctttcc cggcggccgc cggttccatt
    13501 tcctcgatcc cggcggcaac gaactggcgg cggtgaccgc ggacccgtga cccgaggctg
    13561 accgcacctt cggcggtgga cgtccgcagg cttcaccggc cctgcagcta tcccgcaagg
    13621 ccggtccccg gcgaaggccg gggcgcagcg tcggggctcc aggggctcgg tcccggcctt
    13681 cgccgggatg cggttgcaat ccgggggccg ggcccctaga tggccgcttc gcccgtcggc
    13741 gcggacgcat agctcagttg gtagagcagc tgactcttaa tcagcgggt
//
LOCUS       DASVQZ010000006         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_1504,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000006 DASVQZ010000000
VERSION     DASVQZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="VF547_00225"
     CDS             complement(<1..1701)
                     /locus_tag="VF547_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX8261279.1"
                     /translation="MSLIEKIDSPEAGEIEPGKRLIRVGGGQGASLGEHLSSILHRLS
                     WRTPFHALRLRGRHPLKLLAVPKDPVAGDKAAGEAILHGKFVRRGEAFAIESLDFDDP
                     SLPADVCDYIHSFEWLRDLAAAATRERASKLAEKVVHKWLGSHAETIAEPAWRADLWG
                     RRMLFWTAYAPYILSSRDSAYRAVLLNTLSRGARHLDKAAEKARPGLPRITAWAGVIA
                     AALVVQGGPARLGRGESGLLRALAASLHDDGGLVSRSPEEQLMLVELLAQLRAVYYAG
                     ERDMPEAVAEGLSGAVGALLGATLGDEALSSWQGGNMASRRRIAAAIEGSGVRVRPLR
                     QPRGWGYQRLEAKSTVALFDAAPPPPTRAFAGGCASTLAFELSEGPNRLVVNCGGPGR
                     AAGALPAELVHALRSSAAHSTLTLGDRNSTAVHDDGSLGKGVSQVELVRDETAGIVMV
                     EASHDGYVRRFGLVHQRQLTLAADGRELRGQDLLTLQGRRRRPDPIPYSVRFHLHPDV
                     EAATTADGQGALLRVRGRTVWQFRCRGGRLAIEDSLWIDGNCALRETSQLVVAGESPA
                     D"
     gene            complement(1698..>2033)
                     /locus_tag="VF547_00230"
     CDS             complement(1698..>2033)
                     /locus_tag="VF547_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX8261280.1"
                     /translation="LGKKAGVSLNPATPAKMLDYVLEEVDLVLVMSVNPGFGGQSFID
                     SQLRKIEALRKQIDKQGLAVDLQVDGGIDVATARRAVEAGADVLVAGTATFRGGADVY
                     AANIEALRG"
BASE COUNT          313 a          711 c          720 g          289 t
ORIGIN      
        1 gtccgcgggg ctttcgccgg ccaccaccag ctgcgacgtc tcgcgaagcg cgcaattgcc
       61 gtcgatccac aggctgtcct cgatcgcgag ccggccgcca cggcaccgga actgccagac
      121 cgtccgtccc cggacccgga gcagcgcgcc ctggccgtcg gcggtggtcg ccgcctcgac
      181 atccggatgg agatggaagc ggaccgaata agggatcgga tccggccgcc gccgccggcc
      241 ctggagggtg agaagatcct ggccgcgaag ctcgcggccg tcggcggcga gggtgagctg
      301 gcgctggtgg acgaggccga agcgccggac gtagccgtcg tggctggcct ccaccatgac
      361 gataccggcg gtctcgtcgc ggaccagctc gacctgcgag acccccttgc caaggcttcc
      421 gtcgtcatgg accgcggtcg aattgcggtc gcccagagtc aaagtggaat gggcggcgct
      481 gctcctcagc gcatggacga gctccgccgg aagcgctccg gccgcccggc ccgggccgcc
      541 gcaattgacg acgagccggt tcgggccttc cgacagttcg aaggcgaggg tggaggcgca
      601 gccgcccgcg aaggcccggg taggcggcgg cggagcggca tcgaacaggg cgaccgtgct
      661 cttcgcctcg agccgctgat agccccagcc gcgcggctgg cgaagcggcc ggacccggac
      721 tccgctgccc tcgatcgccg ccgcgatccg gcgcctcgag gccatgttgc cgccttgcca
      781 gctggacagg gcctcgtcgc ccagggtcgc tccgagcagc gctccgaccg ctccggaaag
      841 gccttcggcc acggcctcgg gcatgtcgcg ctcgccggca taatagacgg cccgaagctg
      901 ggcgagcagc tcgaccagca tgagctgctc ttccggggag cggctgacga ggccgccgtc
      961 gtcgtgaagc gacgccgcga gcgccctgag caggcccgac tcgccgcggc ccaggcgggc
     1021 cggcccgccc tgaacgacga gcgcggccgc gatcaccccc gcccaggcgg tgattcgagg
     1081 caggcccggt cgcgcctttt ccgccgcctt gtcgagatgg cgggcgccgc gcgaaagagt
     1141 gttgagaagc accgcccggt aggcggagtc gcggctcgac aagatgtagg gcgcataggc
     1201 cgtccagaag agcatgcgcc ggccccacaa atcggcccgc caggcgggct cggcgatcgt
     1261 ctcggcatgg gatccgagcc atttgtggac gaccttctcg gccagcttgg aggcccgctc
     1321 ccgggtcgcg gcggcggcga ggtcgcgcag ccattcgaag ctgtggatgt aatcgcacac
     1381 gtccgccgga agggacggat cgtcgaagtc gagagattcg atcgcgaagg cctcgccacg
     1441 gcggacgaat ttgccgtgca ggatggcctc gccggccgcc ttgtcgcccg ccaccggatc
     1501 cttgggcacg gcgagcagct tgagcggatg ccggccgcga aggcgaaggg cgtggaacgg
     1561 cgtccgccac gacaggcgat ggaggatcga ggagagatgc tcgccgagcg acgctccctg
     1621 tccgccgccg acccggatca gcctcttgcc cggctcgatc tcgccggctt cggggctgtc
     1681 gatcttctcg atcaggctca tcctcgaagg gcctcgatat tggcggcata gacatccgcg
     1741 ccgccgcgga aggtggcggt gccggcgacg aggacgtcgg cgcccgcctc gactgcccgg
     1801 cgcgccgtcg cgacgtcgat cccgccgtcc acctggagat ccacggcgag gccctgcttg
     1861 tcgatctgct tgcgcagagc ctcgattttg cgaagctggc tgtcgatgaa gctctgcccg
     1921 ccgaagccgg gattgacgct catcaccagc acgagatcga cctcttccag aacataatcg
     1981 agcatctttg ccggcgtcgc gggattgagc gaaacgccgg ctttcttgcc gag
//
LOCUS       DASVQZ010000007         7429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_387126, whole genome shotgun sequence.
ACCESSION   DASVQZ010000007 DASVQZ010000000
VERSION     DASVQZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387126"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF547_00235"
     CDS             <1..587
                     /locus_tag="VF547_00235"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8261281.1"
                     /translation="TAATGMDALSHCLEAYCAPGFHPMADGIAAEGMRLIREYLPRAV
                     AAGSDIDARGHMLVAAAMGATAFQKGLGGMHALSHPIGALVGTHHGLTNAVLMPYLLA
                     FNRKAVEERLGRLAGYIGLADASFDGFLAWIVELRRRLAIPHGLAELGVGESDLDRIA
                     AMAEADPSAAGNPRPFGAREARSVLEAAMAGRLG"
     gene            complement(592..1686)
                     /locus_tag="VF547_00240"
     CDS             complement(592..1686)
                     /locus_tag="VF547_00240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8261282.1"
                     /translation="MDRRAFLGSAASALALPGCATTSGRIAAAPTGCLPPVRVDQSRI
                     IRTVAGLRPYRTAGFVVRAEPLGDKRLVHNYGHGGAGITLSWGSSRLAAELGLRGHQG
                     PVAVIGAGVMGLTTARLVQEAGFPVSIYAEALTPHTTSNIAGGQISPYGHFEEDSVTP
                     EWRAQFAAAMDYSWRRFQILVGDDYGIRWLPTYQQTASGGPRPAHPFRPGIRRLSPAE
                     HPFAVEHMILYNTMYVETGRFLRQLTRDVQIAGGRIAVRRFATPADIAALPETLVFNC
                     TGLGARDLFGDKDLHPVRGQLAILLPQPEIRYAVQAQGGYMFPRADGILLGGTFERDE
                     WEAAPQPEAIASIVASHKAMFEGFRCASAA"
     gene            complement(1709..3157)
                     /locus_tag="VF547_00245"
     CDS             complement(1709..3157)
                     /locus_tag="VF547_00245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8261283.1"
                     /translation="MPSPLLPLLAVAFLLAGCGGGGEGYEPEIRDREKRIGAEQHPQL
                     LGEFGGAYAAPEAAYARRVGERIAAAAGLEGECTFTLVNSDVVNAFAVPGCYIYVTRG
                     LMGIVNSEAELASILGHEVGHIVAAHNRRQQRRSLWRNLGVMAVGMVTGSERLTRIAG
                     RAAEYFTLRYSRKQEYEADDLGVRYLAAAGYDPHAASDMLATLGRHEQYMSRTRGRDE
                     ARGIPEWARTHPLAGNRVERAAATAAATGFADDALPEHETAFLREVDGLLYGDDPAQG
                     FVIGRRFAHPLMRIGFEAPPGFTLTNSPQAILIDGPDGLRGEFAGGAIPPGGLEAYAE
                     AVFAALLRGVPAQAAPAQRVRINGVPALLTTVRVQTRDGAIELALAAYDGGGGKAYHF
                     LLVAPPADAAHAAIGALAGSFRLLSADEARSLRPRRIRTVRVTAGENAASIARHMASE
                     HPLDHFLMLNGRSAGEPLRPGELVKIVALAPG"
     gene            complement(3170..4417)
                     /locus_tag="VF547_00250"
     CDS             complement(3170..4417)
                     /locus_tag="VF547_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX8261284.1"
                     /translation="MTAGPVAWSAERLDWTDEPLGEIGLPKGTMRLRSGFGSGLSTRP
                     GDPPGIVWAIGDRGPNLKVKAAAKLYGVEGLERLRDVEGAKIMPRPDLGPALARLRIA
                     DGRVEIVGGFRIADGRGRPVSGLPIPGGPHISCEPAFGLDGAPLDSDPSGLDTEGVAA
                     LPDGGFWVGDEFGPSLVRLGPDGRVAVRLVPEGVALEGAAYPVEARLPAIAARRQINR
                     GFEAVALSPDGSALFLAFQSPLAHPDEQAHERGRHVRLWRLDPATGEVTAQFLYPLDP
                     PEAFRRDRAKGPLGWSDLKVSELCFAGPGSLLVLERGSETTKIYSVSPSEGFELDPSH
                     LDPATRPTVEQLSARDEGPGLPVLAKRLLLSTDEAPDIPADLEGMAILSPSELLLVND
                     NDFGVEGGATSFWRIRFDRPVLV"
     gene            complement(4414..4956)
                     /gene="hslV"
                     /locus_tag="VF547_00255"
     CDS             complement(4414..4956)
                     /gene="hslV"
                     /locus_tag="VF547_00255"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX8261285.1"
                     /translation="MESWHGTTILAVRKNGRAVIAGDGQVTLGQTVIKPNARKVRRLG
                     DGGVIGGFAGATADAFTLFERLERKLEQHHGALMRAAVELAKDWRTDKYLRNLEAMMI
                     VADRDVTLVLTGNGDVLEPGSGVAAIGSGGNFALAAATALYDYEADAEAIARKAMAIA
                     ADICVYTNGNLTVESLEASA"
     gene            5168..5368
                     /locus_tag="VF547_00260"
     CDS             5168..5368
                     /locus_tag="VF547_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261286.1"
                     /translation="MLKRLSNALPAVLAAAPSVIGAVKQVKQALKKPTKLAASADAEA
                     EAAADSGAPPRRLSADSEQTAR"
     gene            5379..>7429
                     /locus_tag="VF547_00265"
     CDS             5379..>7429
                     /locus_tag="VF547_00265"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX8261287.1"
                     /translation="MELLLLLGLAGLAAYALSLASRVKRLEEQVAHWYGEPEPAGPDP
                     LFDSVRAPAAARPAPPRTVPPELIQRAEGPEDLEAAKGPETEPLVGETLGGFFERWVG
                     GRLMIWVGGVALAVAGVLLVRYSIQIGLITPPVQMGMAALFGLLLLALGEFARSRADW
                     TLDRRVAQALVGAGIFVLYATAYGSLVLHQLIGNGTAFGLMVMVTGAALILSLRHGAP
                     SAVMGLVGGFLTPMLVGERSETATPLLTYLALLDVALFTLAGRRGWTWLAAAAALLSF
                     GWTGYLLSWDRPDALAAGVFVVLVGIGASVLRAGNGWQLDFLRPAAIGLVQLAVLVGR
                     VDIGLPAWGLFGILAAACLFLEPRKPEYRPLPIVALSLALLLIAVKAFDQPRHIPAIA
                     AAVTLLFAAGSLPAALRRERPLLPFLTASGALLGPAAILRALRPEWLDRPAWGLLFLL
                     LAAGPIFLAWSRRAAAGVGASDLPLGAAAAAAMLLLGLAAADLLPPVLVPAAWLAIAL
                     GAALAARQWRDPGLVVLVLLAAAVASLAAAERVPQLWAVSLLSLLGVPALASDLPSAG
                     LALLVLLLPAILLAAIWRFLPAPFPRLRSAPLALAGLFAAAGLYLLFKQAFGLSSDED
                     FVARGLAERTIVNQALFAAGWLVCSGRIPVPGLDQRSRWLAGTLLTALAAARLLWFDM
                     L"
BASE COUNT          944 a         2644 c         2737 g         1104 t
ORIGIN      
        1 tcaccgcggc caccggcatg gacgccttgt cccactgcct ggaggcctat tgcgcgcctg
       61 gcttccaccc gatggccgac ggcatcgccg cggagggcat gaggctgatc cgggaatatc
      121 tgccgcgcgc ggtcgccgcc ggctcggaca tcgacgcccg gggccacatg ctcgtcgccg
      181 cggcgatggg cgccaccgcc ttccagaagg ggctcggcgg catgcatgcg ctttcccatc
      241 cgatcggcgc cctcgtcggc acccaccacg gcctcaccaa cgccgtgctc atgccctatc
      301 tgctcgcgtt caaccggaag gcggtcgagg agcggctggg ccggctcgcc ggctatatcg
      361 gcctggccga tgcttcgttc gacggcttcc tggcctggat cgtcgagctt cggcggcgcc
      421 tcgccattcc ccacggcctg gccgagctgg gcgtcggcga gtccgatctc gaccggatcg
      481 cggcgatggc cgaagccgac ccctccgccg ccggcaatcc gcgcccgttc ggcgcccgcg
      541 aagcgcgcag cgtcctcgag gccgcgatgg cggggcggtt gggatagcgg cctaggcggc
      601 cgaagcgcag cggaagccct cgaacatcgc cttgtgcgac gcgacgatgc tcgcgatcgc
      661 ctccggctgc ggcgccgcct cccactcgtc gcgctcgaac gttccgccga gcaggatgcc
      721 gtcggcgcgc gggaacatat aacccccctg ggcctggacc gcgtagcgga tctccggctg
      781 cggcaggagg atggcgagct ggccccggac cggatgcagg tccttgtcgc cgaacaggtc
      841 gcgggcgccg agcccggtgc agttgaagac cagggtctcg ggcagggcgg cgatgtcggc
      901 gggggtcgcg aaccggcgca ccgcgatccg ccctccggcg atctgcacgt cccgggtgag
      961 ctggcgaagg aagcggccgg tctcgacgta catggtgttg tagaggatca tgtgctcgac
     1021 cgcgaacgga tgctcggccg ggctgaggcg ccggatgccg ggacggaagg ggtgcgcagg
     1081 ccgcggaccg ccgcttgcgg tctgctggta ggtcgggagc cagcggatcc cgtaatcgtc
     1141 gccgacgagg atctggaagc ggcgccagct gtagtccatc gccgcggcga actgggcgcg
     1201 ccactcgggc gtgacggaat cctcttcgaa atggccgtag ggcgagatct ggccgccggc
     1261 gatgttggac gtggtgtgcg gggtcaaggc ctcggcgtag atcgagaccg ggaagccggc
     1321 ctcctggacg agccgcgccg tggtgaggcc catgacaccg gcgccgatga cggcgaccgg
     1381 cccctggtgg ccgcgcagcc caagctcggc cgcgagcctc gacgagcccc aggacaaggt
     1441 gattccggcg ccgccatggc cgtaattgtg gaccagcctt ttgtccccaa gcggctcggc
     1501 ccggaccacg aacccggccg tccggtacgg ccgcaggccg gcgacggtgc ggatgatgcg
     1561 gctctggtcg acccggacgg gcggcaggca gccggtcggc gcggccgcga ttcggcccga
     1621 ggtggtcgcg cagcccggca aggcgagcgc ggaggcggcg gagccgagga aggcgcgacg
     1681 gtccatggcc gcagcttagc cctaccggtc agccaggcgc cagagcgacg atcttgacca
     1741 gctcgccggg ccgcagaggc tcgccggcgg agcggccgtt gagcatcagg aaatggtcaa
     1801 gggggtgctc gctcgccatg tggcgggcga tgctggccgc gttctcgccc gccgtcaccc
     1861 gcaccgtccg gatccggcgc ggacgcaggc tgcgcgcctc gtcggcggag agcagccgga
     1921 acgagccggc cagggctccg atcgccgcgt gcgcggcgtc cgcgggaggc gcaacgagca
     1981 ggaaatgata ggccttgccg ccgccgccgt cataggcggc gagtgcgagc tcgatcgcgc
     2041 cgtcgcgggt ctgcacgcgg accgtcgtga gcaaggcggg cacgccgttg atccggaccc
     2101 gctgggcggg tgcggcttgc gccggcacgc cgcgcagcag cgcggcgaag acggcctcgg
     2161 cataggcctc gagcccgccc ggtggaatcg cgccgccggc gaactcgccg cgcagcccgt
     2221 ccggaccgtc gatcaggatc gcctgcgggc tgttggtcag ggtgaagccc ggcggcgcct
     2281 cgaagccgat ccgcatcagc ggatgggcga agcggcggcc gatgacgaag ccctgcgcgg
     2341 gatcgtcgcc gtagagcagc ccgtcgacct cacgaaggaa ggccgtctca tgttcgggga
     2401 gggcgtcgtc cgcgaagccg gtcgccgccg ccgtcgcggc cgcccgctcg acccggttgc
     2461 cggcgagcgg gtgggtgcgc gcccattccg ggattcctcg agcctcgtcg cggccgcggg
     2521 tgcgggacat atattgctcg tgccggccga gagtcgccag catgtcgctc gcggcatggg
     2581 gatcgtagcc ggccgcggcg aggtagcgga ccccgagatc gtccgcctca tattcctgct
     2641 ttcgcgaata gcggagcgtg aaatattcgg cggcgcggcc ggcgatgcgg gtcagccgct
     2701 ccgatccggt gaccattccg acggccatta cacccaggtt ccgccatagc gagcggcgct
     2761 gctgccggcg gttgtgggcg gcgacgatgt gcccgacctc atggccgagg atcgaggcca
     2821 gctcggcctc gctgttgacg atgcccatca gcccgcgggt gacgtagatg tagcagccgg
     2881 gaaccgcgaa ggcgttgacg acgtcgctgt tgacgagggt gaaggtgcac tcgccctcga
     2941 gccccgccgc ggcggcgatc cgctcgccga cccggcgggc ataggccgcc tcgggcgcgg
     3001 cataggcgcc gccgaactcg ccgagcagct gcggatgctg ctcggcgccg atccgcttct
     3061 cgcggtcgcg aatctcgggc tcgtagccct cgccgccgcc cccgcagccg gcgagcagga
     3121 aggccacggc gaggagcggg aggagcggac tcggcatggg caccccggtt cagaccagca
     3181 caggccggtc gaaacggatt cgccagaagc tggttgcgcc gccctcgacc ccgaaatcat
     3241 tgtcgttgac gaggagaagc tcgctcggcg agaggatcgc catgccttcg aggtcggcgg
     3301 ggatgtcggg cgcctcgtcg gtgctgagca gcagccgctt ggcgagaacc ggcaggccgg
     3361 gcccctcgtc gcgggcgctg agctgctcca ccgtcggccg ggtggcggga tcgaggtggg
     3421 acggatcgag ctcgaacccc tcggaagggg acacggaata gatcttggtc gtctcggagc
     3481 cccgctcgag gaccaggagc gagccggggc cggcgaagca gagctcgctg accttgaggt
     3541 cgctccatcc gagcggcccc ttcgcccggt cgcgccggaa ggcctcgggc ggatcgagcg
     3601 ggtagaggaa ctgggcggtc acctctccgg tcgccggatc gagacgccac agccggacgt
     3661 ggcgcccgcg ctcgtgggcc tgctcgtcgg ggtgggcgag cgggctctgg aaggcgagga
     3721 acagcgcgct gccgtcgggc gacagagcca ccgcctcgaa gccgcggttg atctgccgcc
     3781 gggcggcgat cgccggaagc cgcgcctcga ccggataagc ggcgccttcg agcgccacgc
     3841 cctccgggac cagccgcacc gcgacccggc cgtcggggcc gagccggacc agcgaggggc
     3901 cgaactcgtc gccgacccag aagccgccgt cgggcagcgc ggcaacgcct tcggtgtcga
     3961 ggcccgacgg atcggagtcc agcggggcgc cgtcgagtcc gaaagccggc tcgcagctga
     4021 tatggggccc gccgggaatg ggcaggccgg acaccgggcg gccgcggccg tcggcgatcc
     4081 ggaagccgcc gacgatctcc acgcggccgt ccgcgatccg cagccgggcg agggccggcc
     4141 cgagatccgg ccggggcatg atcttggcgc cctcgacgtc gcgcagcctt tcaaggccct
     4201 cgacgccgta gagcttcgcc gccgccttga ccttgaggtt cggcccgcgg tcgccgatcg
     4261 cccagacgat gccgggcggg tcgccggggc gggtcgagag gccggagccg aagccggacc
     4321 gaagccgcat cgtccccttg ggcaggccga tctcgccgag cggctcgtcg gtccagtcga
     4381 gccgctccgc cgaccaggcg accgggcccg ccgtcaagcc gacgcctcca ggctttcgac
     4441 ggtgagattg ccgttggtgt agacgcagat gtcggcggcg atcgccatcg ccttgcgggc
     4501 gatcgcctcc gcgtcagcct catagtcgta gagcgcggtg gcggcggcga gcgcgaaatt
     4561 gccgcccgag ccgatcgcgg cgactccgga tcccggctcg agcacgtcgc cattgccggt
     4621 caggaccagg gtcacgtccc tgtcggcgac gatcatcatc gcctcgaggt tgcgcagata
     4681 cttgtcggtg cgccagtcct tggcgagctc gaccgcggcc cgcatcagcg cgccgtgatg
     4741 ctgctcgagc ttgcgctcga gacgctcgaa cagggtgaag gcgtcggcgg tggcgccggc
     4801 gaagccgccg atcacgccgc cgtcgccgag ccgccggacc ttgcgcgcat tgggcttgat
     4861 caccgtctgg ccgagcgtga cctggccgtc gccggcgatc accgcccgac cgttcttgcg
     4921 caccgccagg atcgtggtcc cgtgccagct ttccatcgac cgctcccgct cttcgagggg
     4981 gcggatatgg gagccggacg cgagcgatgc aatcgagggc cggcggctgc agcttgagag
     5041 gaaagctcac agtctctgat aggacttttc ctcaatatgg gttcatgtta tgttccctcc
     5101 cgactcgtgg tcctcacggg gcgatgaatt tcgagccagg aaggaagctg ccatgtcgat
     5161 aaggtcgttg ctgaagcgcc tgagcaatgc gctccccgcc gtcctcgcgg cggcgccgag
     5221 cgtgatcggc gcggtgaagc aggtgaagca ggcgctcaag aagccgacga aactggcggc
     5281 ctcggcggac gccgaggccg aagccgctgc cgactccggc gcgccgcccc gacgattgag
     5341 cgctgacagc gagcagaccg cccgctagcc tgcgggcgat ggagcttctc ctccttctcg
     5401 gcctcgccgg cctcgcggcc tacgcgctga gcctggcctc gcgggtgaag cgtctcgagg
     5461 agcaggtggc gcactggtac ggggagccgg agccggcggg tccggacccg ctgttcgact
     5521 cggtccgagc cccggctgcg gcgcgccccg cgccgccgcg gaccgtgccg cccgagctga
     5581 tccagcgagc cgagggcccg gaggatttgg aggcggcgaa gggaccggag accgagccgc
     5641 tggtcggtga gacgctcggc ggcttcttcg agcgctgggt gggcggccgg ctgatgatct
     5701 gggtcggcgg agtcgcgctc gccgtcgccg gcgtgctgct cgtgcgctac tcgatccaga
     5761 tcgggctgat cacgccgccg gtgcagatgg gcatggccgc cctgttcggc ctcctgctgc
     5821 tcgcgctcgg cgagttcgcc cgcagccggg cggactggac gctcgaccgg cgagtcgcgc
     5881 aggcgctggt cggcgccggc atcttcgtcc tctacgcgac cgcttacggc tcgctcgtcc
     5941 ttcaccagct gatcggcaac ggcaccgcct tcggcctgat ggtgatggtc accggcgccg
     6001 ccctgatcct gtcgctgcgc cacggcgcgc cgtcggcggt gatggggctg gtcggaggct
     6061 tcctgacgcc gatgctggtc ggcgagcgaa gcgagacggc gaccccgctg ctcacctatc
     6121 tcgccctcct cgacgtcgcc ttgttcacgc tcgccggccg ccgcggctgg acctggctgg
     6181 cggcggcggc ggctctgctc agcttcggct ggaccggcta cctcctctcc tgggaccggc
     6241 ccgacgccct cgccgccgga gtcttcgtcg tcctcgtcgg gatcggcgcc tcggtgcttc
     6301 gcgccggcaa cggctggcag ctcgacttcc ttcggcccgc cgccatcggc ctcgtccagc
     6361 tggcagtgct ggtcggtcgg gtcgacatcg gcctccccgc ctgggggctg ttcgggatcc
     6421 tcgccgccgc ctgcctgttc ctcgagccgc gcaagcccga atatcggccg ctgccgatcg
     6481 tcgccttgtc gctcgccttg ctgctgatcg cggtgaaggc tttcgaccag ccgcgccaca
     6541 ttcctgccat cgcggccgcg gtgaccctcc tgttcgccgc gggatcgctg ccggcggcgc
     6601 tccggcgtga acggccgctg ctccccttcc tcaccgccag cggcgcgttg ctggggccgg
     6661 cggcgatcct gcgcgcgctg aggccggaat ggctggaccg gccggcctgg ggcctgctct
     6721 tcctgcttct cgccgccggc cccattttcc tagcctggag ccgccgcgcc gcggccggcg
     6781 tgggcgcgag cgaccttccg ctcggcgccg cggcggcggc ggccatgctc ctgctcggcc
     6841 tggccgcagc cgacctcctg ccgccggtgc tggtcccggc cgcctggctg gcgatcgcgc
     6901 tcggcgccgc cttggccgcc cggcagtggc gcgatccggg cctggtcgtc ctggtcctgc
     6961 tcgcggcggc cgtcgcctcg ctggcggcgg cggagcgcgt gccgcagctg tgggctgtct
     7021 cgctcctctc gctgctgggc gtgccggcgc tcgcgtccga cctgccctcg gcggggctgg
     7081 ccctgctggt cctgctgctg cccgcgatcc tgctcgcggc catctggcgc ttcctgccgg
     7141 cgccgttccc gcggttgcgc tcggcgccgc tcgcgctcgc cggcctgttc gcggcggccg
     7201 ggctctacct gctgttcaag caggccttcg gcctgtcgtc ggacgaggat ttcgtcgccc
     7261 gcgggctggc ggagaggacg atcgtcaacc aggcgctgtt cgcggcgggc tggctggtct
     7321 gcagcgggcg aatcccggtc cccggcctcg accagcgatc gcgctggctc gccggcacgc
     7381 tgctgacggc gctcgcggcg gctcggctgc tgtggttcga catgctgat
//
LOCUS       DASVQZ010000008         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_2315,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000008 DASVQZ010000000
VERSION     DASVQZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF547_00270"
     CDS             complement(<1..263)
                     /locus_tag="VF547_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261288.1"
                     /translation="MFTDFTETLPPPRPARPPGTAEDWTIPQRWERFGAEEHRVWDVL
                     FARQRRKLAGRAAAAFESGLEGLRLSRSGIPEFDDLNERLFARS"
     gene            complement(622..1086)
                     /locus_tag="VF547_00275"
     CDS             complement(622..1086)
                     /locus_tag="VF547_00275"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8261289.1"
                     /translation="MLSEGDKVPDVRMVSAEGKDISPADFSGQKLVLYFYPKDDTSGC
                     TAEAQAFTALADEFKNAGTWILGISKDDARSHGRFTEKYGLKVALATDADGSVCEAFG
                     TWTEKSMYGRKYMGIERATFLVDRDGVVKRVWRKVKVPGHAEEVLEAARKLP"
     gene            complement(1089..>2094)
                     /locus_tag="VF547_00280"
     CDS             complement(1089..>2094)
                     /locus_tag="VF547_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HEX8261290.1"
                     /translation="RVRRRVNERRFALGVQLIVAHSDPIEAAEGYSRIAEAAINVLAR
                     AAVAEFEAVHGRVPGSELLVLGLGRLGGEALTHASDLDLVYLFSGTHEAESDGPRPLR
                     ATDYFNRLAPRVTAALSVPTASGPIYDVDTRLRPSGTDGMLAVSLESFARYQSEQAWT
                     WEHMALTRARPLFGSPQGRAALQSVIDATLSRPRDPGRLIADAVAMRAEMAAHKAAKG
                     RFDIKLGAGGLVDLEFAVHTLQLKHNVGLYPHLEDALEELVAAGLVSDDIDPALRLLT
                     RMLVMFRLVSPESDEPPDETKPLVAAACGHSDWNALLAAHDAARQRVAELWRRVASGE
                     "
BASE COUNT          319 a          731 c          723 g          321 t
ORIGIN      
        1 gagcgcgcga acaggcgctc gttgaggtcg tcgaactcgg ggatgccgga tcgcgacagg
       61 cgcaggccct cgaggccgct ctcgaacgcc gccgccgccc ggccggcgag cttgcggcgc
      121 tggcgggcga acagcacgtc ccagacgcga tgctcctcgg cgccgaagcg ctcccagcgc
      181 tgggggatcg tccagtcctc cgccgtgccc ggcggccgcg ccggccgggg aggcggcagg
      241 gtctcggtga aatcggtgaa catcggggtc tctccaatat gaaaaacccc gcccggtggt
      301 ccggacgggg tcggttgaag ctgaggcttg ctaagcgcgc gctattccgg tcccgcccga
      361 gcgattccgt aataataata gctggaagcc gcggcggtga tcgtcgcgag agtgcaggcg
      421 gaggcgcgca tcggcttcat cccgtcaagc tgaaggacgg aagcggtttt gtcaacgaag
      481 ggctgagccg gagctgaacg cccggatgcg tggtccccga tccccgttcg ggctgagcct
      541 gtcgaagccc tgtccttccc gcgaaaggaa aggcccttcg acaagctcag ggcgaacggg
      601 gacgaggtgc gccacggctt ctcagggcag cttccgcgcc gcctcgagca cctcctcggc
      661 atggcccggc accttcacct tgcgccagac gcgcttcacc accccgtccc ggtcgacgag
      721 gaaggtcgcg cgctcgatgc ccatatattt gcggccgtac atcgactttt cggtccaggt
      781 cccgaaggcc tcgcagaccg agccgtcggc gtcggtcgcg agcgccacct tgaggccgta
      841 tttctcggtg aacctgccgt ggctcctggc gtcgtccttg gagatcccga ggatccaggt
      901 ccccgcattc ttgaactcgt cggcgagcgc ggtgaaggcc tgggcctcgg cggtgcagcc
      961 ggaggtgtcg tccttgggat agaaatagag gaccagcttc tggccgctga aatcggcggg
     1021 gctgatgtcc ttgccttccg cgctcaccat ccggacatcc ggaaccttgt cgccttcgct
     1081 cagcatcgtc actctcccga cgccacccgg cgccacagct ccgccaccct ctgccgcgcc
     1141 gcgtcgtgcg ccgcaagcaa tgcgttccag tcggaatggc cgcaggcggc cgcgaccagc
     1201 ggcttggtct cgtccggcgg ctcgtccgat tccggcgaca ccagccggaa catgaccagc
     1261 atccgggtca gcagccgaag ggcgggatcg atatcgtccg agacgaggcc ggccgcgacc
     1321 agctcctcga gcgcgtcctc gagatgcggg tagaggccga cattgtgctt cagctgcagc
     1381 gtgtggacgg cgaattcgag gtcgaccagg ccgccggcgc cgagcttgat gtcgaaccgt
     1441 cccttcgccg ccttgtgcgc cgccatctcg gcccgcatgg cgaccgcgtc ggcgatcagc
     1501 cggcccgggt ccctcggcct gctcaaggtc gcgtcgatca ccgactgcag cgcggcgcgg
     1561 ccctgcggcg agccgaagag gggccgcgcc cgggtcagcg ccatatgctc ccaggtccag
     1621 gcctgctcgc tctgatagcg ggcgaagctt tcgaggctga cggccagcat cccgtcggtg
     1681 ccggaaggcc gcagccgggt gtcgacgtcg tagatcggcc ccgacgcggt cggaacgctg
     1741 agcgccgccg tcacccgcgg cgccagccgg ttgaaataat cggtcgcgcg cagcggccgg
     1801 ggaccgtcgc tctccgcctc gtgggtgccg ctgaacagat agacgagatc gaggtccgag
     1861 gcgtgggtca gggcctcgcc gccgaggcgc cccagcccga ggacgagaag ctccgagccc
     1921 ggcactcggc cgtgcacggc ctcgaactcg gcgacggcgg cgcgggcgag gacgttgatc
     1981 gccgcctcgg cgatccgtga atagccttcc gccgcctcga tcgggtcgga atgggcgacg
     2041 atcagctgca cgccgagcgc gaaccggcgc tcgttgaccc ggcgccggac ccgg
//
LOCUS       DASVQZ010000009         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_195133, whole genome shotgun sequence.
ACCESSION   DASVQZ010000009 DASVQZ010000000
VERSION     DASVQZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195133"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF547_00285"
     CDS             complement(<1..187)
                     /locus_tag="VF547_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261291.1"
                     /translation="MRPNLFLIAALALAGCSSEPAPPPPPARTAKAAPAGPPVRVPAE
                     IAAFYRGRGNRPVWVARG"
     gene            complement(1009..1296)
                     /locus_tag="VF547_00290"
     CDS             complement(1009..1296)
                     /locus_tag="VF547_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261292.1"
                     /translation="MRNLVVTLAGLVLTATLAFNATPASAADARCEALPQQIRGALAD
                     ADPAVAKNAARRLKTGEALCRANNERAAAKEFQVALKLLGADGTGTTLAGK"
     gene            1377..2321
                     /locus_tag="VF547_00295"
     CDS             1377..2321
                     /locus_tag="VF547_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8261293.1"
                     /translation="MKQEYIIARNQLDGVEAFLRVAERRSFRAAADDLGLSPSAISQT
                     IRALEGRVGVPLLTRTTRSVGLTEAGERFLEHAQPAFAGLTAAFEAARSFGERPAGLL
                     RLNVPRAAIPFLIEPVIEGFAAAYPEVEVEICGEDELIDLVERGFDAGIRMGDYLEAD
                     MIGIRLTPPFRLVVAGSPDYFERHGRPSVPADLRGHRCIRARAPSTGAIFRWHFDVGG
                     QPLEIAVDGPLIVNDLDVSKAAALRGIGLAYLAEPLVAEHLAAGRLELALADYAAWSP
                     GAYLYYPSRTQMLPKLRAFIDFIRQHRPPERFLQAATA"
     gene            complement(2366..>3035)
                     /locus_tag="VF547_00300"
     CDS             complement(2366..>3035)
                     /locus_tag="VF547_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8261294.1"
                     /translation="RTIALLDEKDRMLGAIGHDLRTPLASLRIRAENMGPEEERERLV
                     ATVEEMTATLDDILTLARTGRAREPVRPVDIAALAEAVAEELRDLGQPVSCEPSPRAV
                     LAVQANLLRRALRNLLDNAVRHGGGGRVAVEARDDRVEIRVEDVGPGIPPERLDEVLE
                     PFRRLETSRNRESGGAGLGLAIAHAVALAHGGRLTLANRAGGGLRASLILPKARVPGD
                     APKP"
BASE COUNT          435 a         1077 c         1060 g          463 t
ORIGIN      
        1 cgccgcgcgc cacccagacc ggccggttgc cccgcccccg gtagaaagcg gcgatctcgg
       61 cggggacccg caccggcggc ccggccgggg cggccttggc cgtccgggcc ggcggcggcg
      121 gcggcgccgg ttccgagctg cagcccgcaa gcgccagcgc ggcgatcaga aaaaggttcg
      181 gacgcaaggg catcgccctc cgatagcatg cccgcccgcc cccgctaaga cccctatcct
      241 cccggatcgg gacgccgaac gatctttggt ccggctggcg tcaagctgga ggatgtggcg
      301 ccagagccgg agccagctcg ccgaggtccg cggggctcag cactcgcggc agatcacccg
      361 agacggagcg cacaacgggc ggcggcgcat ggcgtgcccg cattcaagct cgacgaagat
      421 tattccctgc ccggccccgg ccaccgcggt gacggcgcgg ctgcgcggat cgcgcggctg
      481 atcggtcgat ttcactgctt cccccctcag agaagtcgtt ctctgtcaat gacgtagcag
      541 caattgctac gaacgggaag agcgtctgcg catcgcgcat gacgcgccga gacgagagtc
      601 cgccgatgga gcggaccgac gggggacggc tagcgagggc gtgcgcgctt gccgtccatc
      661 acattggctt gattgtcgta cgcctcggcc agacggagca gcgcatttcg aacatcgccg
      721 gccgtggctc tctccctctc gcgacgagcc tccgccgcca gcctccgata gtcttctgcc
      781 gagtgcatgg gacgctcaat gcgggccgga gcggaaggtt gctgtccgat gctcatccga
      841 acggtttccc cgaacggatc ggcggcgttt agtggaaggg gcgcccgccc cctgtaagaa
      901 cgggcgcccg gcgccggaga taaaggcgcc gggctgagaa cgggtcgggc gtccgttcgt
      961 ttcagtgtgg tccgcgaaat atgattacgc ctgtggccac gcggaggatc atttcccggc
     1021 cagagtggtc cccgtgccgt ccgctccgag cagcttcagg gcgacctgga actccttggc
     1081 ggcggcgcgc tcgttattgg cgcggcagag ggcctcgccg gtcttgaggc ggcgggcggc
     1141 gttcttcgcg acggccgggt cggcgtcggc gagcgcgccg cggatctgct gcggaagcgc
     1201 ctcgcagcgc gcatcggcgg ccgaagcggg cgtggcgttg aaggcgagcg ttgcggtgag
     1261 gacgaggccg gcgagggtca cgacgagatt gcgcatggtc aattccttcc ctgattgtcg
     1321 gggccgagat agacgcgctg gatttctctg ataatccgct ataatatcga tgggccatga
     1381 aacaggaata cataatcgcc cgcaaccagc tcgacggggt cgaggctttc ctgcgcgtcg
     1441 ccgagcggcg cagcttccgc gccgcggccg acgacctggg cctctccccc tcggcgatca
     1501 gccagacgat ccgggccctg gagggacggg tcggagtgcc cttgctgacc cgaaccaccc
     1561 gaagcgtcgg cctgaccgag gcgggcgagc gcttcctcga gcacgcccaa cccgccttcg
     1621 ccgggctcac cgcggcgttc gaggcggcgc gaagcttcgg cgagcggccc gccgggctcc
     1681 tccggctcaa cgttccgcgg gccgccatcc ccttcctgat cgagccggtg atcgagggct
     1741 tcgcggccgc ctatcccgag gtcgaggtcg agatctgcgg cgaggacgag ctgatcgacc
     1801 tcgtcgagcg cggcttcgac gcgggcatcc ggatgggcga ctatctcgag gcggacatga
     1861 tcgggatccg gctgactccg cccttccggc tggtcgttgc cggctcgccc gattatttcg
     1921 aacggcacgg acggccttcg gtgcccgcgg acctgcgcgg ccatcgctgc atccgggctc
     1981 gggccccgag caccggcgcg atcttccgct ggcatttcga cgtcggcggc cagccgctcg
     2041 agatcgcggt cgacggcccg ctgatcgtca acgacctcga cgtcagcaag gcggcggcgc
     2101 tgcgcggcat cggcctcgcc tatctggccg agccgctcgt cgccgagcat ctggcggccg
     2161 gccgcctcga gcttgccctg gccgactatg cggcctggtc gccgggcgcc tatctctatt
     2221 atccgagccg gacccagatg cttcccaagc tgcgcgcctt catcgacttc atccgccagc
     2281 accgcccgcc cgagcgcttc ctccaggccg caaccgcgta gaatcgccga ctgaaaatcg
     2341 gttcgaatcg gtgccagcca cctgatcagg gcttgggcgc gtctccggga acccgtgcct
     2401 tcggcaggat caggctcgcg cgcagccctc ccccggcgcg gttcgccaga gtgagccggc
     2461 cgccatgggc gagcgccacc gcatgggcga tggcaaggcc gaggccggct ccgccgctct
     2521 cccgattgcg cgaggtctcg aggcggcgaa agggctcgag cacctcatcc agccgctcgg
     2581 ggggaatccc cggcccgacg tcctcgaccc ggatctcgac ccggtcgtcc cgcgcctcga
     2641 ccgccaccct gccgccgccg ccgtggcgga cggcattgtc gaggagattg cgaagcgcgc
     2701 ggcggagcag attggcctgg accgcgagca cggccctcgg cgacggctcg caggacaccg
     2761 gctggccgag atcgcgaagc tcctccgcca ccgcctcggc cagggcggca atgtcgaccg
     2821 ggcggaccgg ctcgcgcgcc cggccggtgc gcgcgagggt caggatgtcg tcgagcgtcg
     2881 ccgtcatctc ctcgacggtg gcgaccagcc gctcgcgctc ctcctcgggg cccatgttct
     2941 cggcgcggat gcggagcgag gccagcggcg tgcggaggtc gtgtccgatc gctccgagca
     3001 tgcggtcctt ctcgtcgagc agggcgatcg tccgg
//
LOCUS       DASVQZ010000010         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_2550,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000010 DASVQZ010000000
VERSION     DASVQZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VF547_00305"
     CDS             <1..559
                     /locus_tag="VF547_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8261295.1"
                     /translation="QVVPEGLGLLPEHFRRRLQVTQQCRAEDIEEVRARYSALGIPAE
                     LATYISDMPDKLAWTHLVIARAGASTIAELTVAGRPAILIPLPSATDDHQTVNAREMA
                     KAGGARTIAQKSFTPVELAKQMQKLGLEPAALANAAARARAVGRPQAASDLADLVELT
                     GRDLGADPLPAEEMILTPIPSGAYA"
     gene            574..1986
                     /gene="murC"
                     /locus_tag="VF547_00310"
     CDS             574..1986
                     /gene="murC"
                     /locus_tag="VF547_00310"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX8261296.1"
                     /translation="MKGVAKDIGTIHFVGIGGIGMSGIAEVMHILGYKVQGSDVAEGY
                     VVEGLRKAGIPVRLGHHPDNLGDAAVVVISSAIKKDNPELVCAYERRIPVVRRAEMLA
                     ELMRLKSTVAVAGTHGKTTTTSMIAAMLDAGGIDPTVINGGIINAYGSNARLGSSDWM
                     VVEADESDGSFLRLDGTLAVVTNIDPEHLDHYGSFEKVKDAFVEFVENVPFYGAALLC
                     VDHPEVQTIIPRLRDRRVVTYGFAALADVRGENVRPVAGGNLFDVAIRDRAGEVRTIA
                     DIELPMPGRHNVQNALAAIGVAAELGIDDSTIARGFERFDGVKRRFTRVGEIEGATII
                     DDYGHHPTEIRAVLSAAREGAEGRVIAVVQPHRYTRLRDLMAEFQGAFNDADIVFVTP
                     VYPAGEDPIEGVDAEALAEGLKQRGHRAVKAVADLEDLCLSLRDIAARGDMVICLGAG
                     DITRWAAGLADGIACARAAK"
     gene            2150..3049
                     /gene="murB"
                     /locus_tag="VF547_00315"
     CDS             2150..3049
                     /gene="murB"
                     /locus_tag="VF547_00315"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX8261297.1"
                     /translation="MSDVIDIPSLKGTAERRGSLADFIWFRTGGPAEWLVRPNDAQDL
                     ARFLARLDPSVPVLPVGVGSNLIVRDGGVPGVVVRLPKAMSHVHVEPGNRVRAGGGAM
                     GITVASKARDAHIAGLEFLRGIPGTAGGAVRMNAGAYGREVADILVEATLVLRDGRIE
                     TWPAERLGYTYRHSEVPEGAVVVEALFEGAPGERAAIGAEMDRIAAEREASQPLRSRT
                     GGSTFKNPPGTKAWKLIDEAGCRGLALGDAQVSEKHCNFLLNLGSAKAADIETLGEEV
                     RRRVKAHSGVELEWEIQRVGVAE"
     gene            3152..>3406
                     /locus_tag="VF547_00320"
     CDS             3152..>3406
                     /locus_tag="VF547_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8261298.1"
                     /translation="MDKLHVAVLMGGWSAEREVSLRSGADVVSALESLGHRVTPIDMG
                     RDVAERLREAAPDVVFNALHGTPGEDGTVQGLMDLMGLKYT"
BASE COUNT          540 a         1155 c         1222 g          489 t
ORIGIN      
        1 gcaggtggtg ccggaagggc tcggactgct cccggagcat ttccgccgcc gactccaggt
       61 gacccagcaa tgccgggcgg aggacatcga ggaggttcgc gcccgctatt cggcgctcgg
      121 catcccggcc gagcttgcca cctacatttc ggacatgccc gacaagctcg cctggactca
      181 cctcgtcatc gcccgggccg gcgcctcgac catcgccgag ctgaccgtcg cgggccggcc
      241 ggccatcctc atcccgctgc cgtcggccac cgacgaccat cagacggtca acgcccggga
      301 gatggccaag gcgggcgggg cccggacgat cgcccagaag agcttcacgc cggtcgagct
      361 ggccaagcag atgcagaagc tcgggctcga gcccgccgcg ctcgccaacg ccgcggcgcg
      421 ggcgcgggcg gtcggccgtc cgcaggcggc gagcgacctc gccgacctgg tcgagttgac
      481 cggccgcgac ctcggcgccg atcccttgcc ggccgaggag atgatcctca ctccgatccc
      541 gagcggagcc tatgcctgat ggcgaagcgc gggatgaaag gggtcgccaa ggacatcggc
      601 accatccatt tcgtcggcat cggcggcatc ggcatgtccg gcatcgccga ggtgatgcat
      661 atcctcggct acaaggttca gggctccgac gtcgccgaag gctatgtggt ggaagggctt
      721 cgcaaggccg gcattccggt caggctcggc caccatcccg acaatctcgg cgacgccgcg
      781 gtggtggtga tctcgtcggc gatcaagaag gacaatccgg agctggtctg cgcctacgag
      841 cggcgcattc cggtggttcg gagggccgag atgctggccg agctgatgcg gctgaagtcg
      901 accgtggcgg tggccgggac ccacggcaag acgacgacga cctcgatgat cgcggcgatg
      961 ctcgacgccg gcgggatcga tccgaccgtg atcaacggcg gcatcatcaa cgcctacggc
     1021 tccaacgccc ggctcggcag ctcggactgg atggtggtcg aggccgacga gagcgacggc
     1081 agcttcctga ggctcgacgg caccctggcg gtggtgacca acatcgatcc cgagcatctc
     1141 gaccattacg gctcgttcga gaaggtcaag gacgccttcg tcgagttcgt cgagaacgtg
     1201 cccttctacg gcgcggccct gctctgcgtc gatcatcccg aggtgcagac gatcatcccg
     1261 cgcctgcgcg accggcgggt ggtgacctac ggcttcgccg cgctcgccga cgtccgcggc
     1321 gagaacgtcc ggccggtggc gggcggcaat ctgttcgacg tggcgatccg cgaccgcgcc
     1381 ggggaggtgc gcaccatcgc cgacatcgag ctgccgatgc cggggcgcca caacgtgcag
     1441 aacgcgctcg ccgcgatcgg ggtcgcggcg gagcttggaa tcgacgattc gacgatcgct
     1501 cgcggcttcg agaggttcga cggggtcaag cggcgcttca cccgggtcgg cgagatcgag
     1561 ggcgcgacga tcatcgacga ctacggccac cacccgaccg agatccgcgc cgtcctctcc
     1621 gccgcgcgcg agggggccga gggccgggtc atcgcggtcg tccagccgca ccgctacacc
     1681 cgcctcaggg acctgatggc ggagttccag ggggcgttca acgacgccga catcgttttc
     1741 gtgacgcccg tctatccggc cggcgaggac ccgatcgagg gggtcgacgc cgaagccctg
     1801 gcggaagggc tcaagcagcg cggccaccgg gcggtcaagg cggtcgccga tctcgaggac
     1861 ctctgcctca gcctgcgcga catcgcggcg cgcggcgaca tggtgatctg cctgggcgcg
     1921 ggcgacatca cccggtgggc ggcgggcctc gccgacggca tcgcctgcgc gagggcggcg
     1981 aaatgagcgg ccgcccgcca aatccgatcc ctgcgtcttc ggtgccaggc accgagcgcc
     2041 atgcctttga ttcgattgcg cacccattcc gggaaaatcc gaagtccgct ttttcggtgc
     2101 caggcaccga agcctatgtc gttgaaaagg cggcgggcgg ggcggcggaa tgagcgacgt
     2161 catcgacatt ccgagcctaa aaggcacggc cgagcggcgc ggatcgctgg cggacttcat
     2221 ctggttccgg accggcggcc cggccgagtg gctggtccgg cccaacgatg cccaggacct
     2281 cgcccgcttc ctcgcgcggc tcgatccctc ggtgccggtc ctcccggtcg gggtcggctc
     2341 gaacctgatc gtccgcgacg gcggcgtgcc cggtgtcgtc gtccggctgc ccaaggcgat
     2401 gagccatgtc catgtcgagc ccggcaaccg ggtgcgggcg ggcggcggcg cgatgggcat
     2461 aaccgtcgcg tccaaggccc gcgacgcgca catcgccggg ctcgaattcc tgcgcggcat
     2521 cccggggacc gcgggcggag cggtgcggat gaacgccggc gcctatggcc gcgaggtcgc
     2581 cgacatcctc gtcgaggcga ccctcgtgct tcgcgacgga cggatcgaga cctggccggc
     2641 cgagcggctg ggctacacct accggcacag cgaggtgccc gagggggccg tcgtcgtcga
     2701 ggccctgttc gaaggcgctc ccggcgagcg cgcggcgatc ggcgccgaga tggaccggat
     2761 cgccgccgag cgcgaggcga gccagccgct caggagccgc accggcggct cgaccttcaa
     2821 gaatccgccc ggcaccaagg cgtggaagct gatcgacgag gccggctgcc gcggcctggc
     2881 cctcggcgat gcccaagtct ctgaaaagca ttgcaacttc ctgctcaact tgggcagtgc
     2941 gaaggccgcc gacatcgaga cgctgggcga agaggtgcgg cggcgggtga aggcgcattc
     3001 gggcgtcgag ctcgaatggg aaatccagcg tgtaggagtg gcggagtgac cttcatcgtc
     3061 atcccagcga aagctgggat ccatgcgacc tcagggcagg tcgccgagcc ggcatggacc
     3121 ccagccttcg ctggggtgac ggtgaacggc cgtggataag ctccacgtcg ccgtcctgat
     3181 gggcggctgg tccgccgagc gggaagtgtc gctgaggagc ggggcggacg tggtgtcggc
     3241 gcttgagagc ctcggccacc gggtcacccc gatcgacatg ggccgggacg tcgccgagcg
     3301 gcttcgcgag gccgcgcccg acgtcgtgtt caacgccctt cacgggactc ccggcgagga
     3361 cgggacggtc cagggcctga tggacctgat gggcctcaaa tatacc
//
LOCUS       DASVQZ010000011         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_2654,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000011 DASVQZ010000000
VERSION     DASVQZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2654"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF547_00325"
     CDS             <1..313
                     /locus_tag="VF547_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HEX8261299.1"
                     /translation="QRCGLTLAEIEAELARLPLGRAPTRSDWTAISTRLRTRLDERIA
                     MLEATRDRLDGCIGCGCLSLEACALYNPADRAARAGPGPRFLLGDRAADFAVPRPSPR
                     T"
     gene            complement(401..1582)
                     /locus_tag="VF547_00330"
     CDS             complement(401..1582)
                     /locus_tag="VF547_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8261300.1"
                     /translation="MFFSFVDELRAAGIPASMKEHLVLLEALDADVIASSPEQFYFLA
                     RATFVKDEGLLDRFDQVFGKVFKGLEADYGPDSAPIPEDWLRKVAELYLSEEEMAKIR
                     SLGSWEEIMDALRKRLEEQEGRHQGGSKWIGTAGTSPFGAYGYNPEGIRIGQEGGRNR
                     SAIKVWDKREFRNLDSQVELGTRNIKVALRRLRRFAREGAADELDLDETIDGTARKGW
                     LDIRMRPERHNAVKLLLLLDVGGSMDPHVKVCEELFSAAHSEFKNLEFFYFHNCPYES
                     LWKDNRRRFAERTPTWDVLHKFGHDWKIVFVGDASMSPYEITHPGGSVEHHNEEAGAV
                     WMKRFADTYPSAVWLNPVPEKYWSYSQSTQIVRQLMSDRMYPLTLEGLDDAMRALSRK
                     V"
     gene            complement(1643..2683)
                     /locus_tag="VF547_00335"
     CDS             complement(1643..2683)
                     /locus_tag="VF547_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261301.1"
                     /translation="MGSFWRALAAALVLAAAPAASQPQPRVLEVPATKSWQHAATGMI
                     LPPTAAGLSRGSLVDRTAAEQDVSADYGGEDGLTTSVYIFQTPLPDPALWADRAFAAI
                     LLRPEFGLDPAAPAAAAAPYRRPGAAAAGGIRATFKLDGSGLTATALAVVPLQGWLVK
                     VRMTSARLDPAALDARMTAFLEALRWPAEAKPAPAAAPVVPCRDSLAFKKAKIVPNDM
                     GNALMDAISGTVELDQVEEGKADPVVYCREPGPHAPIGLYRPDGRRDSYVIALGDSGR
                     ALRLAPALTLDLSSGGSAGRRVAMTLLEHDRTTVLPSFNRLPSPEQAMAVAFSGRPRE
                     TISITTTPGEKD"
     gene            2860..>4171
                     /locus_tag="VF547_00340"
     CDS             2860..>4171
                     /locus_tag="VF547_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8261302.1"
                     /translation="MASSAAEQPDGNAINIVARNCGALAIECSDVSGYVSGVSERISS
                     NLRTLDSLEEVTSRLLLDQARVAESTDEARILAEQARERLSEGRAAIEDTIGIFAGLT
                     DLVVQLGERMTGFAEAMSRVQNVSSSIERIAYRTNMLALNATIEAARAGEAGRSFAVV
                     AAEVKKLAQETRTATSEITGTIASLTREAEAVTSEIENGVGRSRVARSGFATITETVR
                     QVADIVALVDRQTDGIAQSTSQIQLSVDSVKSGLSSFAADARDNGGQLLQVHGRLSRL
                     ELLSNEMLDRLASSGVRIDDTPFIEFAQAGMREIRDIIEAAIERGETRADDVFDTRYV
                     PMPGTDPVQYETRFCDFADAHVRPVLDKLMALEPRLIACAITDINGYLPTHISERSQP
                     QSDDPAWNAKYCRNRRNFLDDVTRRAIASEKEAMLATYSMDLGQG"
BASE COUNT          657 a         1486 c         1404 g          624 t
ORIGIN      
        1 ccagcgctgc ggcctgaccc tcgccgaaat cgaggcggag ctggcccggc tgccgctcgg
       61 ccgggcgccg acccgctcgg actggacggc gatcagcacc cgcctccgca ctcggctgga
      121 cgagcggatc gccatgctgg aagcgacgcg cgaccggctc gacggctgca tcggctgcgg
      181 ctgcctgtcg ctggaggcct gcgctctcta caacccggcc gaccgggccg cccgggccgg
      241 gccggggccc aggttcctgc ttggggacag agcggcggac ttcgccgtcc cgcgaccgtc
      301 cccccggact tgatcgggga gccgccttgt cgctccacgg cagcaaagaa ggtggatgcc
      361 ggatcaagtc cggcatgacg acgcacgggc cccacggggt ctagaccttc cggctcagcg
      421 cccgcatcgc gtcgtcgagg ccctcgaggg tgagggggta catgcggtcg ctcatcagct
      481 gccgcactat ctgggtcgac tggctgtagc tccaatattt ctccggcacc gggttgagcc
      541 acacggcgct gggataggtg tcggcgaagc gcttcatcca caccgccccg gcctcctcgt
      601 tatgatgctc gaccgagccg cccggatggg tgatctcgta ggggctcata gaggcgtcgc
      661 cgacgaagac gatcttccag tcgtggccga acttgtggag cacgtcccag gtcggcgtgc
      721 gctcggcgaa gcggcggcgg ttgtccttcc acaggctctc ataggggcaa ttgtggaagt
      781 agaagaattc gagattcttg aactcgctat gggcggcgga aaacaactcc tcgcagacct
      841 tgacgtgcgg gtccatcgag ccgccgacgt cgagcagcag cagcaatttc accgcattgt
      901 gccgctcggg ccgcatgcgg atgtcgagcc agcccttgcg cgcggtgccg tcgatcgtct
      961 catcgaggtc gagctcgtcg gccgcgcctt cgcgcgcgaa gcgcctgagg cgccgcagcg
     1021 cgaccttgat gttgcgggtg ccgagctcga cctggctgtc gaggttcctg aactcgcgct
     1081 tgtcccagac cttgatcgcg ctccggttgc ggccgccttc ctggccgatc ctgatcccct
     1141 cgggattgta gccataggcc ccgaagggcg aggtccccgc ggtgccgatc cacttgctcc
     1201 cgccctggtg gcgtccctcc tgctcctcga gcctcttcct gagagcgtcc atgatctcct
     1261 cccaggagcc gagcgagcgg atcttcgcca tctcctcctc gctgagatag agctcggcga
     1321 ccttcctcag ccaatcctcg gggatcggcg ccgaatccgg gccgtaatcg gcctccagcc
     1381 ccttgaagac cttgccgaac acctggtcga accggtcgag cagcccctcg tccttcacga
     1441 aggtcgcccg ggcgaggaaa tagaattgct ccgggctcga ggcgatgacg tcggcgtcga
     1501 gcgcctcgag gagcacgaga tgctccttca tcgaggccgg gatcccggcc gcccgaagct
     1561 cgtcgacgaa ggagaagaac atggccccgg cctctagcgc gccccgccgg gcagagccag
     1621 cgccggttcc ggacggccgg gcctaatctt tctccccagg ggtggtggtg atcgagatcg
     1681 tctccctggg ccggccggaa aaggcgaccg ccatcgcctg ctccggcgag gggagccggt
     1741 tgaaggaggg aaggaccgtc gtccggtcat gttcgagcag ggtcatcgcc acccgccttc
     1801 cggccgagcc tcccgaagac aggtcgagag tcagcgccgg cgccagccga agcgcgcgac
     1861 cggagtcgcc caaagcgatc acgtaggagt cgcgccgccc gtccggccgg tagaggccga
     1921 tgggcgcgtg cggcccgggc tcgcggcaat agacgaccgg gtcggccttg ccctcctcga
     1981 cctggtcgag ctcgacggtt cccgagatgg cgtccatcag tgcgttgccc atgtcgttgg
     2041 gaacaatctt ggccttcttg aaggcgaggc tgtcgcggca gggaacgacc ggcgccgccg
     2101 ccggcgccgg cttcgcctcg gcgggccagc gcagcgcctc caggaaggcg gtcatccggg
     2161 cgtcgagcgc ggcggggtcg agccgcgcgg aggtcatccg gaccttgacc agccagccct
     2221 ggagcgggac cactgcaagc gctgtggcgg tcagccccga tccgtccagc ttgaacgtgg
     2281 cccggatgcc gcccgccgcc gccgcccccg gccgtcggta gggcgccgcg gccgcggccg
     2341 gggcggcggg atcgagcccg aattcgggcc ggagcagaat tgccgcgaaa gcccggtcgg
     2401 cccacaaagc ggggtcgggc aggggggtct ggaagatgta gacgctggtc gtcagcccgt
     2461 cctcgccgcc gtaatcggcg ctgacgtcct gctccgccgc ggtgcgatcg acgaggctcc
     2521 cacggctgag gccggcggcg gtcgggggca ggatcatccc cgtcgccgca tgctgccacg
     2581 acttcgtcgc cggcacctcc agcacccgcg gctgcggctg gctcgccgcc ggcgccgccg
     2641 ccagaaccag agccgccgcc agcgccctcc aaaagctgcc catcgccgcc ctcgtgcaaa
     2701 ttgccgtcac gcactggtgt cggggactcg tcccggcggg gcaagtgcta atcatggggc
     2761 gaggcgctca agggccgctc cgcaaggcgg gctccttggt taacggcgtt tcaaccattt
     2821 cggcttagcc cttcatcgcg aaacaggcag gggcggcacg tggcgagttc agcggcagag
     2881 caacccgacg gcaatgcgat caacatcgtc gcccgcaatt gcggggcgct ggcgatcgag
     2941 tgcagcgacg tctcgggcta cgtctccgga gtctcggagc ggatctcgtc caacctgagg
     3001 accctcgatt cgctcgagga ggtgaccagc cggctgctgc tcgaccaggc ccgggtcgcc
     3061 gaatccaccg acgaggcgcg gatcctcgcc gagcaggcgc gcgagcgcct gtcggagggg
     3121 cgggccgcga tcgaggacac gatcggcatc ttcgccggcc tgaccgacct cgtcgtccag
     3181 ctcggcgagc ggatgaccgg cttcgccgag gcgatgagcc gggtccagaa cgtctcgtcg
     3241 agcatcgagc gcatcgccta ccgcaccaac atgctggccc tgaacgcgac gatcgaggcg
     3301 gcccgcgccg gcgaggcggg gcgctcgttc gcggtcgtcg ccgccgaggt caagaagctt
     3361 gcccaggaga cccggaccgc gaccagcgag atcaccggca ccatcgcctc gctgacccgc
     3421 gaggccgagg cggtgacctc cgagatcgag aacggcgtcg gccgaagccg ggtcgcccgg
     3481 agcggcttcg cgacgatcac cgagacggtc cgccaggtcg ccgacatcgt cgccctggtc
     3541 gaccgccaga ccgacggcat cgcccagtcg accagccaga tccagctcag cgtcgactcg
     3601 gtcaagtcgg gcctgtcgag cttcgccgcc gacgcccgcg acaatggcgg gcaattgctc
     3661 caggtccatg gccgactctc caggctcgaa ttgctgtcga acgagatgct cgaccgcctc
     3721 gccagctcgg gcgttcggat cgacgacacg cccttcatcg agttcgccca ggccggaatg
     3781 cgcgagatca gggacatcat cgaagccgcc atcgagcgcg gcgagacccg cgccgacgac
     3841 gtcttcgaca cgcgctacgt gccgatgccc ggcaccgacc cggtccagta cgagacccgc
     3901 ttctgcgatt tcgccgacgc ccacgtccgc cccgtgctcg acaagctgat ggcgctcgag
     3961 ccgcggctga tcgcctgcgc catcaccgac atcaacggct atctgcccac ccacatctcc
     4021 gagcggtcgc agccgcagag cgacgatccg gcgtggaacg ccaaatattg ccgcaaccgc
     4081 cgcaacttcc tcgacgacgt cacccgccgg gcgatcgcca gcgagaagga ggcgatgctc
     4141 gccacctact cgatggacct cggccagggc c
//
LOCUS       DASVQZ010000012         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_580532, whole genome shotgun sequence.
ACCESSION   DASVQZ010000012 DASVQZ010000000
VERSION     DASVQZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580532"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VF547_00345"
     CDS             <1..509
                     /locus_tag="VF547_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261303.1"
                     /translation="AAALVACTPSPQDTAMPRQSPDSGKPAQASPQAAVERAGSTAGG
                     VDKTPAAIGPIPQPVSAAENPTPSGPADSPASAQGRRILSTAFVRVGPDGRLTVERRD
                     GRVLVLRDVVMRPGDYCGVHVIGGPAGSKYCGGYAEVAAARPGGGPAPEAPDLAGPNP
                     VKSPPKGT"
     gene            complement(547..622)
                     /locus_tag="VF547_00350"
     tRNA            complement(547..622)
                     /locus_tag="VF547_00350"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(587..589),aa:Thr,seq:tgt)
     gene            736..1689
                     /locus_tag="VF547_00355"
     CDS             736..1689
                     /locus_tag="VF547_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEX8261304.1"
                     /translation="MGGRIGTAWLGAALLLGAAPPPEHTAPWPGLVAGTTRSEIQDSV
                     DRGPSLERGRQARGELVEHRRLDAALGALLPQRKNVVDAYVVVAALDSDPVFAREARE
                     AGRVLARRFDAEGRTLVLAGSDGRGAATLPGGSLHALTLALARAAELMDSQEDVLVLY
                     TTSHGAPYGLTYHDGDEGYGILSPARLKVLLGELGIRNRLLMISACYSGVFVPLVASD
                     TTAVVTAASDSRTSFGCQADNDWTFFGDALVNRALRTPQTLDLAAAEALRLIAQWEKA
                     KGYKASYPQVSVGSGVGAWLKPLEARMPKTATAPVGRPSVE"
     gene            complement(1693..2571)
                     /locus_tag="VF547_00360"
     CDS             complement(1693..2571)
                     /locus_tag="VF547_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261305.1"
                     /translation="MDRSPDRFSHFAAIDWSGARGERQKGIAVAICGGGREAPRLVRP
                     DIGWSREAALRWMLEDMPEGTLVGLDLGPSLPFEDRGAFFPGWEDSPPDARSLWAFVE
                     THCAADPHLSASTFVDHPEAARHFRRHGGRCGDLFEAGRGRLRVTEVAQREQGLNPYS
                     NFNLVGAAQVGKSSLTGMRVLHRLDGRLPIWPFDPVPPSGSLVVEIYTSLAAIAAGLP
                     KNRTKIRDPETLDLVLAALGSDPHAPLARYDDHSTDALISAAWLRLIHTDPRVWQPPR
                     LTPELARTEGWTFGVL"
     gene            complement(2571..3368)
                     /locus_tag="VF547_00365"
     CDS             complement(2571..3368)
                     /locus_tag="VF547_00365"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEX8261306.1"
                     /translation="MAPRISISRSVGMIVFFLIMIPVTISALDALRIQAVSGPATQML
                     RTILNSIPLVIGALILLALGYIIGKLVKVGIEQILPTMGFDRSVSAIGISAEGPAPSR
                     TVGTAAMVGIMIFFAIKAAEMLQSPIIAAMLAQVLELGSHVLFGAVIILAGVAIARIV
                     SNLVSGAGTQGWLPTILRWAIIALSVAIGLRFMGLANEIVIIAFASIIGSAAVACALA
                     FGLGGRPTAHKLLERWTDANRVPAAPVPPRAPTAPPPPMSDSPPPLI"
BASE COUNT          521 a         1171 c         1260 g          451 t
ORIGIN      
        1 ccgccgccgc gctcgtcgcg tgcacgcctt cgccccagga caccgccatg ccccgacaaa
       61 gtcccgattc gggaaagccg gctcaagctt cgccgcaagc cgccgtcgag cgcgcggggt
      121 cgacggcggg cggcgtcgac aagacgccgg ccgcgatcgg gccgatcccg cagccagtgt
      181 ccgcggcgga gaatcccacc ccgagcggcc ccgcggattc gcccgcctcg gcacaggggc
      241 ggcggatcct ctccacggcc tttgtgaggg tgggccccga cggccgcctg acggttgagc
      301 gccgcgacgg gcgcgtgctg gtccttcgcg acgtcgtgat gcgccccggc gattattgcg
      361 gcgtgcacgt aatcggcggc ccggcgggct cgaaatattg cggcgggtac gccgaggtcg
      421 ccgcggcccg gccgggcggc ggccctgcgc cggaggcgcc ggaccttgcc ggtccgaacc
      481 ccgtaaagtc ccctccgaag gggacctagg ctgaaggcga acgggcgccc gcgcagacta
      541 ttgaggtggt gccggctgtc ggactcgaac cgacgaccta ccgcttacaa ggcggttgct
      601 ctaccagctg agctaagccg gcccggcccg ttccatgggt caaaaccgct tgaaggtcca
      661 ccctcacgcg aagccgttga cggagccccg gcggctcgcc gaagatcggc gccggcggac
      721 ggaacgggga atcggatggg cggcaggatc gggacggcat ggctgggggc cgcgctgctg
      781 ctgggggcgg cgccgcctcc cgagcacacg gcgccgtggc cgggcttggt cgccggcacg
      841 acccgctccg agatacagga cagcgtcgac cggggacctt cgctggaacg cggccgccag
      901 gcgagaggtg aactggtcga gcaccgccgc ctcgacgccg cgctgggcgc gctcctcccg
      961 caacgcaaaa acgtggtgga tgcctatgtc gtcgtggccg cgctggacag cgatccggtg
     1021 ttcgcccgcg aggcgcggga agcgggcagg gtgctcgctc ggcgcttcga tgccgagggc
     1081 cgcacgctcg tcctcgccgg cagcgacggg cgaggcgccg ccacgctgcc cggcggcagc
     1141 ctgcacgcgc tcactctggc gctcgctcgc gccgccgaac tgatggacag ccaggaggac
     1201 gtgctggttc tctacacgac gagccacggg gccccgtacg gcctgaccta ccatgacggc
     1261 gacgaaggct atggcattct cagtccggcg cgcctcaagg ttttgctcgg cgagctgggc
     1321 atccgcaacc ggctgctgat gatctccgcc tgctattcgg gcgtgttcgt ccccttggtg
     1381 gcgagcgaca cgacggcggt ggtgacggcc gcttccgaca gccgcacctc gttcggctgt
     1441 caggctgaca atgactggac gtttttcggc gacgcgctgg tgaaccgcgc gctcaggacg
     1501 ccgcagacgc tcgatctggc ggcggcggag gcgcttcggc tgatcgcgca gtgggaaaag
     1561 gcgaagggat ataaggcttc ctacccgcag gtcagcgtcg ggtctggcgt gggcgcctgg
     1621 ctgaagccgc tcgaggcgcg catgccgaag accgcgacgg cgccggtcgg gcggccctcg
     1681 gtcgagtgaa ggtcagagca cgccgaacgt ccagccttcg gtgcgggcca gctcgggcgt
     1741 gaggcgcggc ggctgccaga cacgcgggtc ggtgtggatg aggcgcagcc acgcggccga
     1801 aatcagcgcg tcggtgctat gatcgtcgta gcgggcgagc ggcgcgtgcg gatcgctgcc
     1861 gagcgccgcc agcaccaggt cgagcgtctc cgggtcgcgg atcttcgtac ggttcttcgg
     1921 gagcccggcg gcgatcgccg cgaggctcgt gtagatttcc acgaccagcg agccggaggg
     1981 cgggacaggg tcgaacggcc agatcggcag gcgcccgtcg agccggtgga ggacgcgcat
     2041 gccggtcagg ctcgacttgc cgacctgcgc cgccccgacc aggttgaagt tgctgtaggg
     2101 gttgaggccc tgctcgcgct gcgcgacttc cgtgaccctc agccggccgc ggccggcctc
     2161 gaacaggtcg ccgcagcggc cgccgtggcg gcggaagtgc cgcgccgcct cgggatgatc
     2221 gacgaaggtc gaggcggaga ggtgcgggtc ggcggcgcaa tgcgtctcga cgaaggccca
     2281 gaggctgcgt gcgtcgggcg ggctgtcctc ccagccggga aagaaagcgc cgcggtcctc
     2341 gaaggggagg gaagggccga ggtcgagccc gacaagagtc ccctccggca tatcctccag
     2401 catccagcga agcgccgcct cccgcgacca gccaatgtcc ggccgcacga ggcggggcgc
     2461 ctcgcgcccg ccgccgcaga tcgcgacggc gatgcccttc tgccgctccc ccctggcgcc
     2521 ggaccagtcg atcgccgcga agtgcgagaa acgatccggc gagcgatcca ctagatcagc
     2581 ggcggggggc tgtccgacat cggcggcggc ggagccgtgg gcgcgcgcgg cggcacgggc
     2641 gccgccggga cccggttcgc atccgtccag cgctccagca gcttgtgcgc ggtcggccgc
     2701 ccgcccagcc cgaaggcgag cgcgcaggcg accgccgccg agccgatgat ggacgcgaag
     2761 gcgatgatga cgatctcgtt ggcgaggccc atgaagcgca gcccgatcgc gaccgagagc
     2821 gcgatgatcg cccaccgcag gatggtcggc agccaccctt gcgtgcccgc tccgctgacg
     2881 aggttcgaga cgatccgcgc gatggcgacg ccggcgagga tgatcaccgc cccgaacaac
     2941 acgtggctgc cgagctcgag gacctgcgcc agcatcgccg cgatgatcgg cgactggagc
     3001 atctccgccg ccttgatcgc gaagaagatc atgatcccca ccatcgccgc cgtgccgacc
     3061 gtgcgcgagg gcgccggccc ttccgcggag atgccgatgg cgctgacgga gcggtcgaag
     3121 cccattgtcg gcaggatctg ctcgatgccg accttgacga gcttgccgat gatgtagccg
     3181 agcgcgagca ggatcagcgc gccgatcacc agcgggatcg agttgaggat agtgcgcagc
     3241 atctgcgtcg ccggacccga caccgcctgg atgcgcagcg cgtcgagcgc cgagatcgtc
     3301 accgggatca tgatcaggaa gaagacgatc atgcccacgg agcggctgat cgaaatgcgc
     3361 ggggcgatcg acggcgccgc gcccgccggg ctggcgggga cgg
//
LOCUS       DASVQZ010000013         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_387935, whole genome shotgun sequence.
ACCESSION   DASVQZ010000013 DASVQZ010000000
VERSION     DASVQZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387935"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF547_00370"
     CDS             complement(<1..448)
                     /locus_tag="VF547_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261307.1"
                     /translation="MRIFDSFPFDGELDLLEFRLRENFDQVDHFILVEAAVSYRGAPK
                     RFSFEENAERFAWARAKIRHVKLDGLGPGPTARERAAVQRNASRLALDDSAPDDVVLL
                     LDVDEVPARPLLERLRREGLAEPRRLAMTRHYGFADLLAPRSPCCPT"
     gene            complement(454..1236)
                     /locus_tag="VF547_00375"
     CDS             complement(454..1236)
                     /locus_tag="VF547_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8261308.1"
                     /translation="MKLDSQIAAVVTGGASGLGEATARAFAARGVRVAVFDRDSEKGE
                     RVAAEIGGVFAEVDVTSDEAVAAGFAKARSAHGQERICINCAGVANAAKTVGRDKATG
                     NPTPYPIAQFELVVRVNLIGTFRVLTQSAYGMVGVDPLEDGEKGLIVNTASVAAQDGQ
                     IGQAAYSASKAGVLGMALPIARDLMNEGIRVNTILPGVFETPMVSMMPQNVKDALAAQ
                     VPFPKRLGRPEEYARLALFMAENPYLNGEHVRLDGAIRMAPR"
     gene            1339..1527
                     /locus_tag="VF547_00380"
     CDS             1339..1527
                     /locus_tag="VF547_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261309.1"
                     /translation="MHRLMITCSTDRPWGLHAAVRSDEDCPRCGWTAPGPLGDALAGA
                     REPDGDPVGGSICAPGIE"
BASE COUNT          239 a          567 c          518 g          246 t
ORIGIN      
        1 cggtcgggca gcagggcgag cggggcgcga gcaggtcggc gaaaccgtaa tggcgggtca
       61 tggcgagccg cctcggctcg gccaggccct cgcggcgcag ccgctccagc agcggccggg
      121 cgggcacctc gtcgacgtcc agaagcagca cgacgtcgtc gggcgccgaa tcgtcgaggg
      181 ccagcctcga ggcgttgcgc tgcaccgccg cccgctcccg cgcggtcggc cccgggccga
      241 gcccgtccag cttcacgtga cggatcttcg ctcgcgccca ggcgaagcgc tccgcattct
      301 cctcgaagct gaagcgcttg ggcgcgcccc ggtacgaaac cgccgcctcg acgaggatga
      361 aatggtcgac ctggtcgaaa ttctccctca gccgaaactc cagcaagtcg agctcgccgt
      421 cgaacggaaa ggagtcgaag atgcgcatga cgcctagcgc ggcgccatcc tgatcgcgcc
      481 gtcgagccgc acatgttcgc cgttcagata gggattctcc gccatgaaga gggcgagccg
      541 cgcatattcc tcgggccggc cgagccgttt cgggaagggc acctgcgccg ccagcgcgtc
      601 cttcacgttc tgcggcatca tcgacaccat cggagtctcg aacacgcccg gaaggatggt
      661 gttgaccctg atcccctcgt tcatcaggtc ccgggcgatc gggagcgcca ttccgagcac
      721 gcccgccttc gaggccgaat aggccgcctg gccgatctgc ccgtcctggg cggccaccga
      781 cgcggtgttg acgatcaggc ccttctcgcc gtcctcgagc gggtcgacgc cgaccattcc
      841 gtaggccgac tgggtgagca ctcggaacgt gccgatgagg ttgacccgga cgaccagctc
      901 gaactgggcg atcggatagg gcgtggggtt ccccgtcgcc ttgtcgcgcc ccaccgtctt
      961 ggcggcgttg gcgactcccg cgcaattgat gcagatccgc tcctggccat gcgccgagcg
     1021 ggccttggcg aagccggccg cgactgcctc gtcggacgtc acgtcgacct cggcgaagac
     1081 gccgccgatc tcggccgcga cccgctcccc cttctcggaa tcgcggtcga acacggcgac
     1141 cctcaccccg cgcgcggcga acgcccgggc ggtcgcctcg ccgagaccgg aggcgccgcc
     1201 ggtgacgacg gcggcgattt gactgtcgag cttcatgtag agcctccgct gaacttcgcg
     1261 gacgctccta ggcagtcccg ccgccgccat caacggcccg ctccattttg ttcattgtat
     1321 gcacaggatt gcatagttat gcatcgcctg atgatcacct gctcgaccga tcgcccttgg
     1381 gggcttcatg cggcggtgcg cagcgacgag gattgcccgc gctgcggctg gaccgcgccg
     1441 ggaccgctcg gcgacgcgct cgccggcgcg cgcgagccgg acggagaccc cgtcggcggc
     1501 tcaatttgtg cgcccgggat tgaatgagcc cacccgcggc gccatttcac tccagcccat
     1561 gcagctcatc
//
LOCUS       DASVQZ010000014         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_580877, whole genome shotgun sequence.
ACCESSION   DASVQZ010000014 DASVQZ010000000
VERSION     DASVQZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580877"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VF547_00385"
     CDS             complement(<1..800)
                     /locus_tag="VF547_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX8261310.1"
                     /translation="MTEDLKPKPPLELWGGVECTIVRLGDEYRDQTAETGHLHRRGDI
                     DLIADLGIRTVRYPILWEAIAPERPDDLDWAWTDERLAMLRERGIKVIGGLTHHGSGP
                     KYTELLDPDFPRKLADYAGRVAARYPWIEMWTPVNEPLTTARFSALYGHWYPHTKDYS
                     KFLRALVNECKGTLEAMRAIRAHIPHARLVQTEDLGKCYSTAPLRYQANFENERRWLS
                     LDLLCGAIDPKHPMHRMLIGAGIGWDEIESFAGGEARPDILGINHYLT"
     gene            974..1264
                     /locus_tag="VF547_00390"
     CDS             974..1264
                     /locus_tag="VF547_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261311.1"
                     /translation="MELHEKIGSGTDERRAEPRIEGLIDHATLWFRGEAHEVPVLNIS
                     SRGTMVESALIPRLGEPVLVQFPDCSRIHAFVRWARDGRMGLNFGHEMVLGG"
     gene            complement(1261..1833)
                     /locus_tag="VF547_00395"
     CDS             complement(1261..1833)
                     /locus_tag="VF547_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX8261312.1"
                     /translation="MADQPPLILAFGDSLMAGYGLARDEGFAPQLEAALRGRGVEAEV
                     VNAGVSGSTAGNGRMRLKWVLDALPRPPALAIVSFGGNDLLRGVPARETREDMAAILA
                     VLKARGIPVVLGGMLAPPFLGEAYKKEFNSLFAGLAREYEVAGFYPFFLAGVAGVAGL
                     NLPDRVHPNRDGIARIVSGILPFVLEAVGA"
     gene            1924..2580
                     /locus_tag="VF547_00400"
     CDS             1924..2580
                     /locus_tag="VF547_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8261313.1"
                     /translation="MIDLYTAATPNGWKASIALEELGLPYEVKVLDLGKGEQKAAAFL
                     AINPNGRIPAIVDRDNGDFAVFESGAILLYLAEKTGRLMPADAKGRSRVVQWLMFQMG
                     GVGPMMGQANVFTRYFPEQLPSVIERYRRESRRLFEVLDGHLANHEYLAGDYSIADIA
                     NWSWVHTHAWPGVEVEGLDHLQRWLAAIAARPAVQKGVATPPSAEPSRTVETARSILA
                     "
     gene            2616..>3330
                     /locus_tag="VF547_00405"
     CDS             2616..>3330
                     /locus_tag="VF547_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8261314.1"
                     /translation="MNEITRIAAQPAYSDAARKVLAREPGLFIDGEWVKSSHGRTLPV
                     YDPSTGKEIAAIVDASDADVDKAVAAARRAFDDGRWSGLAPYKRERIMERLAALIEEN
                     IPELAELESIDNGKPRSASQGYDLPKCVQTLRYMAGWATKISGEHVEPAGLPTGSVHA
                     YVRREPIGVCAQIVPWNFPLMMAVQKIAPALAAGCTIVLKPAEQTSLTALRLADLVAE
                     AGIPAGVLNIVTGLGETAGD"
BASE COUNT          549 a         1152 c         1113 g          516 t
ORIGIN      
        1 ctggtgagat agtggttgat gccgaggatg tcgggcctcg cctcgccgcc cgcgaagctc
       61 tcgatctcgt cccagccgat gccggcgccg atcagcatcc ggtgcatggg gtgctttgga
      121 tcgatcgcgc cgcacagcag gtcgaggctc agccagcgcc gctcattctc gaaattggcc
      181 tggtagcgaa gcggcgcggt cgagtagcat ttgcccagat cctcggtctg gacgaggcgg
      241 gcgtgcggga tgtgcgcgcg gatcgcccgc atcgcctcca gcgtgccctt gcactcgttg
      301 accagcgccc gcaggaactt ggaataatcc ttcgtgtgcg ggtaccagtg gccgtagagg
      361 gccgagaagc gcgcggtggt cagcggctca ttgacggggg tccacatctc gatccagggg
      421 taacgcgcgg cgacccggcc ggcatagtcg gcgagcttcc tggggaagtc cggatccagc
      481 aattcggtat atttcgggcc cgacccatga tgggtgaggc cgccgatcac cttgatgccg
      541 cgttcgcgga gcatcgccag ccgctcgtcc gtccaggccc agtcgagatc gtcgggccgc
      601 tcgggcgcga tcgcttccca aaggatcggg tagcgcaccg tccggatgcc cagatcggcg
      661 ataaggtcga tgtcgccgcg ccggtggaga tggccggtct ccgcggtctg gtcgcgatat
      721 tcgtcgccga ggcggacgat cgtgcattcg acgccccccc acaattccag cggaggcttt
      781 ggctttagat cttcggtcat ccggctcgcg ttcgctgttg cggttcagaa acgaaaccgg
      841 cctcggcggg cgccggttcc ggacgaaagc cgtcggttaa agcaagttag cgagatttcc
      901 cgaccgtttc gggccgctag ggaattcgca acgctttgcg gcgcatgctt cccgcctgga
      961 gcggagagcg ccgatggaac ttcacgagaa gatcgggagc ggaaccgacg aacgccgcgc
     1021 agagccgcgg atcgaggggc tgatcgacca cgccactttg tggttccgtg gcgaggcgca
     1081 cgaagtgccg gtgctcaaca tctcctcgcg cgggacgatg gtggagagcg ccctcatccc
     1141 ccgcctcggc gagccggtgc tggtgcaatt ccccgactgc agccgcatcc acgctttcgt
     1201 gcgctgggcg cgcgacgggc gcatggggct caatttcggc cacgagatgg tactcggcgg
     1261 ctaggcgccg accgcctcca atacgaaggg tagaatgccg gacacgatgc gggcgatccc
     1321 gtcgcgattg gggtggaccc ggtcgggcag gttgagaccc gccacgcccg cgaccccggc
     1381 gaggaagaag ggatagaagc ccgccacctc atattcgcga gccaggccgg cgaagaggct
     1441 gttaaactcc ttcttgtacg cctcgccgag gaacggcggc gcgagcatcc ctccgagcac
     1501 gaccggaatg ccgcgcgcct tcagcacggc gaggatggcg gccatgtcct cgcgcgtctc
     1561 gcgcgccggt acgccgcgca gcaggtcgtt gccgccgaac gagacgatgg cgagcgcggg
     1621 cgggcggggc agtgcgtcga gcacccactt cagccgcatc cgcccgttcc cggcggtgct
     1681 gcccgacacg ccggcattga ccacttccgc ctcgacgccc cggccgcgca gcgccgcctc
     1741 gagctggggc gcgaaaccct cgtcgcgcgc caggccgtag cccgccatga ggctgtcgcc
     1801 gaaggcgagg atgagaggcg gttggtcggc catggtcccg ctcaaagctt cagcgggcga
     1861 agagttgcga cggcgcggat taggtcctat ttcgcaaccc acctcttcgc gaaggagcct
     1921 cgcatgatcg acctctacac cgccgccacg cccaacggct ggaaggcctc gatcgcgctg
     1981 gaggagctcg gcctccctta cgaggtcaag gtgctcgacc tcggcaaagg agagcagaag
     2041 gcggccgcgt tcctcgcgat caacccgaac ggccgcatcc cggcgatcgt cgaccgggac
     2101 aatggcgact tcgccgtatt cgagagcggc gccatcctcc tctacctcgc cgagaagacg
     2161 gggcggctga tgccggcgga cgcgaagggg cgcagccgcg tcgtccagtg gctgatgttc
     2221 cagatgggcg gcgtcggccc gatgatgggt caggcgaacg tcttcacccg ctatttcccc
     2281 gagcagctcc cttccgtcat cgagcgctac cgccgcgaga gccgccgcct gttcgaggtg
     2341 ctggacggcc accttgcgaa ccacgaatat ctcgccggcg actattcgat tgccgacatc
     2401 gccaactggt cctgggtcca cacccacgcc tggcccggcg tggaagtgga ggggctcgac
     2461 cacctccagc gctggctggc cgcgatcgcc gcgcgcccgg ccgtgcaaaa gggcgtcgcg
     2521 accccgccca gcgccgagcc gtctcgcacc gtggagaccg ctcgctcgat cctcgcctga
     2581 ccgctataga ccgcccagaa ggagattttc cgcccatgaa cgagattacc cgcatcgccg
     2641 cccagcccgc ttattccgac gccgcccgca aggtgctggc ccgcgagccc ggcctgttca
     2701 tcgacggcga atgggtgaaa tcgagccacg gcaggacgct tcccgtctac gatccctcga
     2761 ccggcaagga gatcgccgcg atcgtcgacg cgtccgacgc cgacgtcgac aaggcggtgg
     2821 cggccgcgcg gcgggcgttc gacgatggcc gctggtcggg cctcgcgccc tacaaacgcg
     2881 aacggatcat ggagcggctc gccgccctca tcgaggagaa catccccgaa ctggccgagc
     2941 tcgaatcgat cgacaacggc aagccgcgca gcgccagcca gggctacgat ctgcccaaat
     3001 gcgtccagac cctgcgctac atggcgggct gggcgaccaa gatcagcggc gagcatgtcg
     3061 aacccgccgg cctgccgacc ggctccgtcc acgcctacgt ccggcgcgag ccgatcggcg
     3121 tctgcgcgca gatcgtgccc tggaacttcc cgctgatgat ggcggtgcag aagatcgcgc
     3181 ccgcgctcgc cgccggctgc acgatcgtcc tcaagcccgc cgagcagacc tcgctgaccg
     3241 cgcttcgcct cgctgacctg gtcgccgagg cggggatccc ggccggcgtg ctcaacatcg
     3301 tcaccggcct cggcgagacg gccggcgacc
//
LOCUS       DASVQZ010000015         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_388855, whole genome shotgun sequence.
ACCESSION   DASVQZ010000015 DASVQZ010000000
VERSION     DASVQZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388855"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="map"
                     /locus_tag="VF547_00410"
     CDS             <1..729
                     /gene="map"
                     /locus_tag="VF547_00410"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX8261315.1"
                     /translation="AGRAAAEVLDALVPHVVPGITTQAIDDIVYREMNARGGIPATLG
                     YRGFTGSCCTSINHVVCHGIPGDRVLKDGDIVNIDVTIILDGWHGDTSRMFLVGDVPI
                     RARRLVDVTYECMMLGIEKARPGNFLGDVGHAIQTHAEKHRYGVVRDFCGHGLGRLFH
                     DAPEVVHAGRPGSGPELRPGMFFTIEPMINIGKPGVKLLEDGWTAVTRDRSLSAQFEH
                     SIGITEDGCEIFTKSPAGLDKPPY"
     gene            1035..>1763
                     /locus_tag="VF547_00415"
     CDS             1035..>1763
                     /locus_tag="VF547_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261316.1"
                     /translation="MRWIVTVGLALSAAVSWAQTPIDQLAKPPADAKRYVIMSTAGKH
                     GEATEWTAADGTRWARASLVLRGQVWEIDEAMKLGTDGMIERYTARGVSPQGDVAESF
                     AVAGGKAEWKSQIDQGSAAYTGPAIYYPAGPGSLSFEHLVERLLASPDRSVALLPGGR
                     AKIEPLAKARIGSGASARTVIAHAITGISNSPIPVWTTEDGKYFASIGFLSNIPVGYE
                     DAQPVLEKAQDEALAARSPALARRF"
BASE COUNT          292 a          622 c          578 g          271 t
ORIGIN      
        1 gccggccgtg ccgcggccga ggtgctcgac gccctcgtcc cgcacgtcgt tccggggatc
       61 acgacccagg caatcgacga catcgtctac cgcgagatga acgcccgcgg cggcattccg
      121 gcgacgctcg gctatcgcgg cttcaccgga agctgctgca cgtcgatcaa ccacgtcgtg
      181 tgccacggca ttcccggcga ccgggtgctg aaggacggcg acatcgtcaa catcgacgtg
      241 accatcatcc tcgacggctg gcacggcgac accagccgca tgttcctggt cggcgacgtc
      301 ccgatccgcg cccgccggct ggtcgacgtc acctacgaat gcatgatgct cgggatcgag
      361 aaggcccggc cgggcaactt cctcggcgac gtcggccacg ccatccagac ccatgccgag
      421 aagcatcgct acggagtcgt ccgcgacttc tgcggccacg gcctcggccg cctgttccac
      481 gacgcgccgg aggtcgtcca tgccgggcgc ccgggcagcg gccccgagct ccgccccggc
      541 atgttcttca ccatcgagcc gatgatcaac atcggcaagc cgggcgtgaa gctgctcgag
      601 gacggctgga cggcggtgac ccgcgaccgt tcgctgtcgg cccagttcga gcacagcatc
      661 ggcatcaccg aggacggctg cgagatcttc acgaagagcc ccgccggcct ggacaagccg
      721 ccttactaga ttccctctcc ctctagggga gagggtaagt tattgctaca cttgaatttt
      781 ccgcacccct ccccatttcc cctccatcgt tcaacaacgg aaaggaggtg gtctgatgtc
      841 tcattgtcat atgccgcgtg cggcgagtcg gatgagcgca gcctccgcgg aggtccgcgc
      901 ctaagacttc aggccgcatt ccggctgaca atggaaaggc cgtcccgcga ggggcggcct
      961 ttcgcaataa ggggcaattg aattgcgggc ggcgcgcgca tagcctcccc ccttggttga
     1021 gaagggggac gccgatgcgt tggatcgtga ccgtcgggct ggcgctgagc gccgccgttt
     1081 cgtgggcgca gacgcccatc gaccaactcg ccaagcctcc cgccgacgcg aagcgctacg
     1141 tcatcatgtc gaccgccggc aagcacggcg aggcgaccga gtggaccgcc gccgacggca
     1201 cccgctgggc gagggccagc ctcgtccttc gcggccaggt ctgggagata gacgaggcga
     1261 tgaagctcgg gacggacggg atgatcgagc gctacacggc ccggggcgtc agcccgcagg
     1321 gagacgtggc ggaaagcttc gccgtcgccg gcggcaaggc ggaatggaag tcgcagatcg
     1381 accagggctc ggcggcctat accgggcccg ccatctatta tccggccggg cccggctcgc
     1441 tctccttcga gcatctcgtc gagcggctcc tcgcctcgcc cgaccgcagc gtggccctgc
     1501 ttccgggcgg tcgggcgaag atcgagccgc tcgccaaggc gcggatcggc agcggcgcct
     1561 cggcgcgcac ggttatcgcc cacgcgatca ccgggatcag caactccccg atcccggtgt
     1621 ggaccaccga ggacgggaaa tatttcgcct cgatcggttt cctctccaac atcccggtcg
     1681 gctacgagga cgcgcagccg gtgctcgaga aggcgcagga cgaggccctc gcggcccgct
     1741 cgccggcgct ggcccgccgg ttc
//
LOCUS       DASVQZ010000016         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_678112, whole genome shotgun sequence.
ACCESSION   DASVQZ010000016 DASVQZ010000000
VERSION     DASVQZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678112"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VF547_00420"
     CDS             complement(<1..975)
                     /locus_tag="VF547_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8261317.1"
                     /translation="MDVQAAEAATATTLVLRDGVILLGAAMLFVMLFRRLGLGAVLGY
                     LVAGALVGPQGLRLVSNPEEMLHIADFGIVLLLFLVGLELHPNRLWRLRHDIFGLGLL
                     QVTLAGLAISAIIYFAAGFSPAASLAIGLPLALSSTAQVLPSLQSDGSINTPAGERAF
                     SVLLFQDLSIVPLITIIAALSRAPADPLTPPGWQLGLYTLGAIAGLVLAGRFLINPLF
                     RLVGRVSERELFIVAGLFIVLAAAAVMETLHLSTALGAFIAGVMLADSPYRHELEVDI
                     EPFRSILLGLFFVAIGMMLDLGAIAQRPLFVIGMAIALIAVKALLLFGI"
     gene            complement(975..1415)
                     /locus_tag="VF547_00425"
     CDS             complement(975..1415)
                     /locus_tag="VF547_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX8261318.1"
                     /translation="MAAPLYTREILRLAASIPHLGDLEGADARADKRSPLCGSRVSVA
                     LSLDGQGRVSAFSQEVHACALGQAAAALLGRHVLGSSLGELETARDALRDFLAGRRDE
                     PGEWPDVSLLASARPFAARHASILLAFDAAAEAAAQAQARRAAA"
     gene            complement(1466..>2114)
                     /locus_tag="VF547_00430"
     CDS             complement(1466..>2114)
                     /locus_tag="VF547_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX8261319.1"
                     /translation="AAPALVRRLVDERLDMVVGARKSEVEEAYRLGHRLGNRLFTGIL
                     ASLFGRTFTDIFSGYRVFSRRFVKSFPALSRGFETETEISVHALELAMPVAEVETDYG
                     ARPEGSHSKLSTWRDGWRIMKTILHLFRIERPVLFYGGFGAFLAAVALVLAVPLIVTF
                     IQIHQVPRFPTAILVTGLMIVAVISFAVGLILDTVVRGRREMRRLAYLALPAVGE"
BASE COUNT          397 a          699 c          770 g          248 t
ORIGIN      
        1 gatcccgaac agcagaagcg ccttcacggc gatgagagcg atcgccattc cgatgacgaa
       61 caacggccgc tgcgcgatcg cgccgagatc gagcatcatt ccgatggcga cgaagaagag
      121 gccgagcagg atcgagcgga acggctcgat gtcgacttcg agctcgtgcc ggtagggcga
      181 gtcggcgagc atgacgccgg cgatgaaggc gccgagcgcg gtcgacagat ggagcgtctc
      241 catcaccgcc gcggcggcga ggacgatgaa gaggccggcg acgatgaaca gctcgcgctc
      301 gctgaccctg cccaccagcc ggaacaaggg gttgatgagg aagcgcccgg ccagcaccag
      361 gccggcaatc gcccccagcg tataaaggcc gagctgccag ccgggcgggg tcagcggatc
      421 ggccggcgcg cggctcaagg cggcgatgat cgtgatcaag ggcacgatcg acaggtcctg
      481 gaacagcagc accgaaaagg cgcgctcgcc cgccggcgtg ttgatcgatc cgtccgactg
      541 caggctgggc aggacctgcg ccgtcgatga caaagcgagc ggcaggccga tcgcgaggga
      601 cgcggccggg ctgaagccgg cggcgaaata gatgatggcg gagatggcga gcccggcgag
      661 cgtcacctgg agcaggccga gcccgaatat gtcgtgccgc agccgccaga ggcggttcgg
      721 gtggagttcc aggccgacca ggaagagaag gaggacgatg ccgaaatcgg cgatgtggag
      781 catctcctcc ggattgctga cgaggcggag cccctgcggg cccaccagag cgccggcgac
      841 gaggtaaccg agcaccgcgc cgagcccgag gcggcgaaag agcatgacga acagcatcgc
      901 cgcgccgagc aggatgacgc cgtccctcag gacgagcgtc gtggccgtcg cggcttcggc
      961 cgcctgcacg tccatcaagc ggcggcccgg cgggcctgcg cctgcgccgc ggcctcggcg
     1021 gcggcgtcga aggcgagcag gatggaggcg tgacgggcgg cgaacggccg cgcggaggcg
     1081 aggagcgaga cgtccggcca ttcgcccggc tcgtcgcggc ggccggcgag aaaatccctc
     1141 agcgcatcgc gggcggtctc gagttcgccg aggctcgatc ccaatacgtg ccggccgagc
     1201 agagccgcgg ccgcctgccc gagcgcgcag gcgtgcacct cctgggagaa ggcgctcacc
     1261 ctcccctgcc cgtcgagcga cagcgcgacg ctcacgcggc tgccgcacaa gggcgaacgc
     1321 ttgtcggcgc gcgcatcggc gccctccagg tcgcccagat gcgggatcga agccgccagg
     1381 cgaagtatct cgcgcgtgta gaggggagcg gccacgagcc tcctataggc gctggggtgg
     1441 cgggcggcca gaagcggtcg gcccgtcact cgccgacggc gggcagcgcc agataggcaa
     1501 gccgccgcat ttcccgtcgg ccgcgcacta ccgtgtcgag gatcagcccg accgcgaagc
     1561 tgatcaccgc cacgatcatc aggccggtga cgagaatggc ggtcgggaag cgcggcacct
     1621 ggtggatctg gatgaaggtc acgatcagcg ggacggcgag caccagcgca acggccgcca
     1681 ggaaggcgcc gaagccgccg tagaagagga ccgggcgctc gatgcggaac aggtgcagga
     1741 tcgtcttcat gatccgccac ccgtcgcgcc aggtcgacag cttcgaatgc gagccttcag
     1801 ggcgggcgcc gtagtcggtc tccacctccg cgaccggcat ggcgagctcc agcgcgtgca
     1861 cgctgatctc ggtttccgtc tcgaagccgc gcgacagcgc cgggaagctc ttgacgaatc
     1921 gtctcgagaa cacccggtaa cccgagaaga tatcagtgaa agtccgcccg aacaggctgg
     1981 cgagaatgcc cgtgaacagg cggttgccga gccggtggcc gagccgatag gcctcttcga
     2041 cttccgactt gcgggcgccc accaccatgt cgagccgctc gtcgaccagg cgccgcacca
     2101 gtgccggcgc cgcc
//
LOCUS       DASVQZ010000017         6267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_485017, whole genome shotgun sequence.
ACCESSION   DASVQZ010000017 DASVQZ010000000
VERSION     DASVQZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485017"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VF547_00435"
     CDS             complement(<1..1359)
                     /locus_tag="VF547_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8261320.1"
                     /translation="MSEKPDYRATVFLPKTDFPMKAGLSQKEPAILARWAEMGLYKRL
                     REARAGRERFILHDGPPYANGDIHMGHAMNKILKDMVVRSQSLLGKDAPYVPGWDCHG
                     LPIEWKIEEQYRKKKLDKDAVPPREFRAECRAYAEKWVAIQSEQFQRLGVMGEWDDPY
                     LTMKYEAEAKIVEELLKFAESGQLYRGAKPVMWSPVEKTALADAEVEYEEITSTQIDV
                     AFEIVESPVSELVGARAVIWTTTPWTIPVNQAIAYGPDIDYSLYLIADSTSYLLIAEA
                     LAEEFQRRTGIGLLDMVPIKGSELAGTVARHPMHHLGGFFARPRPFLPGDFVTTEQGT
                     GLVHMSPDHGEDDFLLCKAHGIDPVFAVAADGKYRDDWLWLGGQGSVINAKFNAPDGP
                     ICSDLREAGALLAASADFKHSYPHSWRSKAKVIFRATPQWFIPMDAQIPSPLAGEGRE
                     GGA"
     gene            complement(1484..2482)
                     /locus_tag="VF547_00440"
     CDS             complement(1484..2482)
                     /locus_tag="VF547_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX8261321.1"
                     /translation="MRIWRWIGSGVVAFAVTVSLINASWIAPRPKGPLILVAHRGLGQ
                     QFDHEGVGRDTCTASRIRPSEHDFIENTLRSMSNARHFGADAIELDVHPTKDGQMVVF
                     HDWTLDCRTDGRGPVRQKTLAELKRLDVGYGYTHDGGRTFPLRGRGVGGMPTVEEALR
                     ETPRMRLFFNFKSKDPRDADGLVAAFRRAGVKIDDKHVFYGHPIVTARLKQLVPGART
                     FWKEEMRACLTDYVKFGWTSYVPESCRGKIVAVPLNYQWAIWGWPKRFLARMESVGTK
                     VILLGPYEKGEIAGIERPEQPPRFRATSAATSGSRISTRSGGRLRIEFPSLNTGRG"
     gene            complement(2479..3405)
                     /locus_tag="VF547_00445"
     CDS             complement(2479..3405)
                     /locus_tag="VF547_00445"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX8261322.1"
                     /translation="MERLDGGSAVPGALRGGIVALGNFDGFHAGHQAVAARAVERAKA
                     EGRPALVATFDPHPVRFFRPETPPFRLTTLDQRQRLFEAAGADAMIVFSFDAGLAATP
                     ADQFISRNLLELIGAAGVVTGEDFTFGRGRGGNIAVLQDAGARLGFSVDAVGPVSDAR
                     GPISSSRIRDALVAGDCGEASRLLTRPFAIEGVVEHGDKRGRTIGYPTANIALGPYLR
                     PRFGIYAVRGRLADGRVVDGAANLGIRPTFEPPRELLEPYFFDFDGDLYGQKIEVELI
                     DFLRPEAKFDSLDALKAQMAADCEEARRRLAR"
     gene            complement(3573..4730)
                     /locus_tag="VF547_00450"
     CDS             complement(3573..4730)
                     /locus_tag="VF547_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX8261323.1"
                     /translation="MRKFVYGLLAVLILAAGGFLLFGPGLVESGMNRVVATPLPAVGD
                     RARALHARLAIADMHADTLLWQRSLLDPARRGQVDLPRLEAGNVALQVFSSVTKTPKG
                     QNYDANGADTDNITLLAVAQLQPPRTWSSLLERSLWHAEKLRRAEAGSEGRLRIVRSG
                     IDVRRLLADRTAGRKVTGALLSIEGLQNMEGRLANLDRLHAAGFRMGGLVHFFDNQVA
                     GSMHGLKKGGLTPLGRQVVRAMEAKGMIVDIAHSSHAAVAEVLAMARRPVVSSHGGVQ
                     ATCQVNRNLTDEEIRGVARTGGVVGIGYWDAAVCDLRPAAIAAAIAHVRDLVGIDHVA
                     LGSDFDGAVTTGFDTAGLVNVTQALIDRGFTDEEIAKVMGGNLLRLLAQLP"
     gene            complement(4733..5224)
                     /locus_tag="VF547_00455"
     CDS             complement(4733..5224)
                     /locus_tag="VF547_00455"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX8261324.1"
                     /translation="MDAPPPVTIVVARASNGAIGLDGGLPWRLPADMKHFKAVTMGTA
                     MVMGRRTFESLPGLLPGRRHIVLTRNPGWKAQGAEVARTTCEARELTGGGPISVIGGA
                     EIIALYLPLADAIELTEVHAEPEADTFLPPFDSHWREVSREDHDAEGERPAFSFVRLE
                     RRG"
     gene            5288..>6267
                     /locus_tag="VF547_00460"
     CDS             5288..>6267
                     /locus_tag="VF547_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX8261325.1"
                     /translation="MRNLVRAIAGLALLLLAAAPAAAQPPLPAPVIAYRGATLLDVRS
                     GALLRGITIVTKGETVEALLPAGAPVPAGAETVDVAGLYAVPGLIDVHQHVENGRGRR
                     AAEAVLRRELYSGVTAVRDMLGDARILADLSRAARFGELAAPDLDFAGFVAGPGYFID
                     DRLAPLARGGMPGQVPWIQAIGPETDLPLAIARIAGTGARGVKVYGNLDGPTVRRIAA
                     EAHRQGLAAWAHAAVFPATPQQVVDAGADSVSHVCMLAYQTLKAPPVSYHDRAPLDQA
                     ALMRGPVHPALARLFESMKARGTVLDTTVAVYHLIERLRVEMPDHPPPTY"
BASE COUNT          938 a         2175 c         2193 g          961 t
ORIGIN      
        1 cgcacccccc tccctgccct cccccgcaag gggggagggg atctgcgcat ccatcgggat
       61 gaaccattgc ggggtggcgc ggaagatgac cttggccttg gagcgccagc tgtgcgggta
      121 ggagtgcttg aagtccgccg aagccgcgag cagcgccccc gcctcgcgca ggtccgagca
      181 gatcggcccg tccggcgcat tgaacttggc gttgatcacg ctgccctggc cgccgagcca
      241 gagccagtcg tcccggtact tgccgtctgc ggcgacggcg aagaccggat cgatgccgtg
      301 cgccttgcag aggaggaaat cgtcctcgcc atgatcgggc gacatgtgga cgaggccggt
      361 gccctgctcg gtggtgacga agtcgccggg caggaagggc cggggccgcg cgaagaagcc
      421 gccgagatgg tgcatcgggt gacgggcgac ggtgccggcg agctcggagc ccttgatcgg
      481 gaccatgtcc aagaggccaa tgcccgtgcg gcgctggaac tcctcggcta aagcctcggc
      541 gatcaacagg tacgaggtcg agtccgcgat caggtagaga ctgtagtcga tgtcggggcc
      601 ataggcgatc gcctgattga cggggatcgt ccaaggggtc gtggtccata tgacggcccg
      661 tgcgccgacc agctcggaaa caggactctc gacaatctcg aacgccacgt cgatctgggt
      721 cgacgtgatc tcctcatatt cgacctcggc atcggccagg gccgtcttct ccaccggcga
      781 ccacatgacc ggcttggcgc cgcggtagag ctggccgctc tcggcgaact tcagaagctc
      841 ctcgacgatc ttcgcctcgg cctcatattt catcgtgaga taggggtcgt cccactcgcc
      901 catcactccg agccgctgga actgctcgga ctggatggcg acccactttt ccgcataggc
      961 tcggcattcc gctcggaatt cccgcggcgg caccgcgtcc ttgtcgagct tcttcttgcg
     1021 gtattgctcc tcgatcttcc actcgatcgg gaggccgtgg cagtcccagc cgggcacgta
     1081 gggcgcgtcc ttgccgagca gcgactgcga gcgcaccacc atgtccttga ggatcttgtt
     1141 catggcgtgg cccatgtgga tgtcgccatt ggcgtagggc gggccgtcgt ggaggatgaa
     1201 gcgttcgcgc ccggcgcgcg cctcgcgcag gcgcttgtag aggcccatct cggcccagcg
     1261 cgcgaggatg gccggctcct tctgggagag gccggctttc atcgggaagt cggtcttagg
     1321 aaggaagacc gtggcgcggt aatcgggctt ttcggacatt gccgggggcc ttagcgcagg
     1381 tagcgcggga attggaagcc gaactcccct tcctgcacgc agggaggggc cggaggtggg
     1441 tgcgttgcct gaagaggcac tgcctgcgga gacccaccca cccctagccc ctccctgtat
     1501 tcagggaggg gaattcaatt ctcaagcgcc cgcccgaccg cgtagaaatc ctcgatccag
     1561 aggtagccgc ggaagtcgcg cggaaccttg gcggctgctc cgggcgctcg atgccggcga
     1621 tctccccttt ctcatagggg ccgagcagga tcaccttggt ccccacgctc tccattcggg
     1681 cgaggaagcg cttgggccac ccccagatcg cccattggta gttgagcggc acggcgacga
     1741 tcttgccgcg gcagctttcc ggcacgtagc tggtccaccc gaacttcaca taatcggtga
     1801 ggcacgcccg catctcctcc ttccagaagg tgcgcgcgcc cggcacgagc tgcttcaacc
     1861 gggcggtgac gatcgggtgg ccgtagaaga cgtgcttgtc gtcgatcttc accccggctc
     1921 ggcggaaagc ggcgacgagg ccgtcggcgt cgcgcgggtc cttgctcttg aagttgaaga
     1981 acaatcgcat ccgcggcgtc tcgcgaagcg cttcctcgac cgtcggcatg ccgccgacgc
     2041 cgcggccgcg aagcgggaag gtccggccgc cgtcgtgggt atagccgtag ccgacgtcga
     2101 ggcgcttcag ctcggccagg gtcttctggc ggaccgggcc gcggccgtcg gtgcggcagt
     2161 ccagcgtcca gtcgtggaag acgaccatct ggccgtcctt cgtcggatgg acgtccagct
     2221 cgatcgcgtc ggcgccgaaa tggcgggcgt tggacatgga gcgcaaggtg ttctcgatga
     2281 aatcgtgctc ggacggccgg atccggctgg cggtgcaggt gtcgcggccg acgccctcgt
     2341 ggtcgaactg ctggccgagg ccgcgatggg cgacgagtat cagcgggccc ttgggccggg
     2401 gcgcgatcca cgaggcgttg atcagcgaga cggtgacggc gaaggcgacg acgccggatc
     2461 cgatccaccg ccagatcctc atcgggcgag cctccgtctc gcctcttcgc aatcggccgc
     2521 catctgggcc ttgagcgcgt ccaggctgtc gaacttcgcc tcaggccgga ggaagtcgat
     2581 cagctcgacc tcgatcttct ggccgtagag gtcgccgtcg aagtcgaaga aatagggctc
     2641 gagcaattcc ctcggcggct cgaaggtcgg ccggattccg agattggccg cgccgtcgac
     2701 gaccctgccg tcggcgagac ggccgcgcac ggcgtagatg ccgaagcgcg gacgcagata
     2761 ggggccgagc gcgatgttgg ccgtcggata gccgatcgtc cggccccgct tgtcgccatg
     2821 ctcgaccacg ccctcgatcg cgaacgggcg cgtcaggagc cgggaagcct cgccgcagtc
     2881 gccggcgacg agagcgtcgc ggatccggct cgacgagatc ggcccccgcg cgtccgagac
     2941 cggcccgacc gcgtcgacgg agaagcccag ccgagcgccc gcgtcctgca gcaccgcgat
     3001 gttgccgccg cggccgcggc cgaaggtgaa gtcctcgccg gtgaccactc cggcggcgcc
     3061 gatcagctcg agcaggttcc gggagatgaa ctggtcggcg ggggtcgcgg ccagcccggc
     3121 gtcgaagctg aagacgatca tcgcatcggc gccggccgcc tcgaacaggc gctggcgctg
     3181 gtcgagcgtg gtcagcctga agggcggcgt ctcggggcgg aagaagcgca ccgggtgggg
     3241 atcgaaggtc gccactagag ccggccggcc ctcggccttg gcgcgctcga cggcgcgggc
     3301 cgccacggcc tggtggccgg cgtggaaacc atcgaaattg ccgagcgcga cgatgccccc
     3361 gcgaagcgcc ccgggaaccg ccgagccgcc gtccagccgc tccatgcgcg cgctatagga
     3421 gagggggcgg aggcccacaa tcctccccgg aacggggagg gggaccatgc gaagcatggt
     3481 ggagggggcc cagcgtccgg gctgcgacga gtccgggccc cctccaccac gtcgcttcgc
     3541 gccgcggtcc ccctccccgt tccggggagg agctagggaa gctgcgccag cagcctcagc
     3601 aaattcccgc ccatcacctt ggcgatctcc tcgtcggtga agccccggtc gatcagcgcc
     3661 tgggtgacgt tgacgagccc ggccgtgtcg aagcccgtgg tcaccgcgcc gtcgaagtcg
     3721 gagccgaggg cgacatggtc gatcccgacg aggtcgcgga catgggcgat ggcggcggcg
     3781 atcgcggcgg gcctcaggtc gcagacggcc gcgtcccaat agccgatgcc gacgacgccg
     3841 ccggtgcggg cgacgccgcg tatctcctcg tcggtgaggt tgcggttgac ctggcaggtc
     3901 gcctggaccc cgccgtggct ggagacgacg gggcgccgcg ccatcgccag cacctcggcg
     3961 acggcggcat ggctcgaatg ggcgatgtcg acgatcatcc ccttcgcctc catggcgcgg
     4021 acgacctggc ggccgagcgg cgtgaggccg cccttcttca gcccgtgcat cgatccggcc
     4081 acctggttgt cgaagaagtg gacgagcccc cccattcgaa acccggcggc atggagacgg
     4141 tcgagattgg cgagccggcc ttccatgttc tggagcccct cgatcgaaag cagggctccc
     4201 gtcaccttgc ggcccgcggt ccggtcggcg agcagccgtc ggacatcgat tccactgcgc
     4261 acgatccgca gccgcccctc ggagcccgct tcggcccgcc gcagcttctc ggcatgccag
     4321 agcgagcgct ccagaagcga gctccaggtc cgcggcggct gcagctgcgc gacggcgagc
     4381 agggtgatgt tgtcggtgtc ggcgccgttg gcgtcgtaat tctgcccctt gggcgtcttg
     4441 gtcaccgacg agaagacctg cagcgcgacg ttgccggcct cgagccgggg cagatcgacc
     4501 tggcctcggc gggccggatc gaggagggac cgctgccaga gcaaggtgtc cgcatgcatg
     4561 tcggcgatgg cgagccgggc gtgaagcgcc cgagcgcgat cgcctacggc gggcagcggc
     4621 gtcgccacca ccctgttcat gccgctctcg acgaggcccg gcccgaacag caggaagccg
     4681 ccggcggcga ggatcaggac ggcgagcagc ccgtagacga atttgcgcat gatcaccccc
     4741 ttcgctccag ccgcacgaag ctgaatgcgg gccgctcgcc ctcggcgtca tgatcctcgc
     4801 gcgagacctc ccgccaatgg ctgtcgaacg gcggcaggaa cgtgtccgcc tcgggctcgg
     4861 catggacttc ggtgagctcg atcgcatccg cgagcggcag atagagcgcg atgatctcgg
     4921 cgccgccgat caccgatatc gggccgccgc cggtcagctc tctggcctcg cacgtcgtcc
     4981 gagcgacctc tgcgccttgc gccttccatc cggggttccg ggtgaggacg atgtgacggc
     5041 gccccggcag cagcccgggc aggctctcga acgtccgccg ccccatcacc atcgccgtgc
     5101 ccatcgtcac cgccttgaaa tgcttcatgt cggcgggaag ccgccagggc aggccgccgt
     5161 ccagcccgat cgcgccgttc gaagcgcggg cgacgacgat cgtgacggga ggcggcgcgt
     5221 ccatcggctt tgcctagcgg ctgacccggg ccggcgctac cttgggacgg aaaaccgagg
     5281 ggagtcgatg cgaaacctcg tccgcgccat cgccgggctt gcgctgctgc tgctcgcggc
     5341 cgccccggcg gccgcccagc cgccgctccc ggcccccgtc atcgcctatc gcggcgcgac
     5401 cctgctcgac gtgcgcagcg gcgcgctgct gcgcggcatc accatcgtca cgaagggcga
     5461 aaccgtcgag gcgctgctcc cggccggagc ccctgtcccc gccggggccg aaacggtgga
     5521 cgtcgccggc ctctacgccg tgccgggcct gatcgacgtc catcagcatg tcgagaacgg
     5581 ccgcggccgg cgcgccgccg aggccgtgct gcgccgcgag ctctacagcg gcgtcaccgc
     5641 cgtgcgcgac atgctcggcg atgctcggat tctcgccgat ctgtcgcgcg cggcgcggtt
     5701 cggcgagctt gccgcgccgg acctggactt tgccggcttc gtggcgggcc ccggctattt
     5761 catcgacgac cggctggcgc ccctcgcccg cggcggcatg ccgggtcagg tgccctggat
     5821 ccaggcgatc gggccggaga ccgacctgcc cctggcgatc gcccgcatcg ccggcaccgg
     5881 cgcgcgggga gtcaaggtct acggcaatct cgacgggccg acggtgcgcc ggatcgccgc
     5941 cgaggcgcac cgtcagggcc tcgcggcatg ggcccatgcc gcggtcttcc cggcgacccc
     6001 gcagcaggtc gtcgacgccg gcgccgacag cgtctcccac gtctgcatgc tggcctatca
     6061 gacccttaag gcgccgccgg tcagctatca cgaccgggcg ccgctcgacc aggcggcgct
     6121 gatgcgcggg ccggtgcatc cggcgctcgc ccgcctgttc gagtcgatga aggcgcgcgg
     6181 caccgtgctc gacaccacgg tcgcggtcta ccatctgatc gagcggctcc gggtcgagat
     6241 gccggaccat cccccgccca cctattg
//
LOCUS       DASVQZ010000018         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_4840,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000018 DASVQZ010000000
VERSION     DASVQZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4840"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VF547_00465"
     CDS             <1..548
                     /locus_tag="VF547_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX8261326.1"
                     /translation="GEAARLWLTEREARLRETAAALKASPEEVPARVAALLDERRRLE
                     RELAEAKKALAMGGGRSAEAQDPEQIGGHGFIGQVLEGMDPKGLRGLVDEAKQRVGSG
                     VVALVAVNDGRATVAVGVTDDLAARRNAVDLVRKAVEAVGGQGGGGRPDMAQGGGPDG
                     ARADEALDAVRRALSEETVAA"
     gene            complement(576..1814)
                     /locus_tag="VF547_00470"
     CDS             complement(576..1814)
                     /locus_tag="VF547_00470"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEX8261327.1"
                     /translation="MTRRSGSADLPLHGGRVPAWLGERMTRLGTVMAEAIILHHGRDA
                     LLARLAHPFWFQSFGAVMGMDWHSSGITTSVIGALKRGLNPRAGELGLQVCGGRGRHS
                     RQTPAELAIAGERAGFDGTALARASRLVAKVDSAAVQDGFDLYLHGFIVADDGKWAVV
                     QQGMNGSSRTARRYHWLSKGLDSFVDSPHAAIEGRRQGEIVNLADRRAVASRRAQLDL
                     LAELGPDRLAREAAALERAPAAEPLQPHLALPGHHEVRPTDVILRRLHGTLAAAAEAG
                     PKDFPELLLVPGVGARTVRSLAMVAEVIHGAPCRFADPARFSLAHGGKDRHPFPVPTK
                     VYDRTISVLKSAVEQAKLGQDEKLGAIRRLDEQARRLEAYVSGPSFEAVMAEERRLSN
                     AYGGRSIFGWEPPAAEAGAA"
     gene            complement(1958..3739)
                     /locus_tag="VF547_00475"
     CDS             complement(1958..3739)
                     /locus_tag="VF547_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8261328.1"
                     /translation="MHADFQTSGFSDALVVLGAAGVVIPAFARFRISPVIGFILVGLL
                     VGPAGLGAMTGQAPWLNYITISNPEAVEPFAEFGIVLLLFAIGLELSFRRLWTMRRLV
                     FGVGAAELLVCALLIGLALHAFGQSWSGSVALGLALALSSTALVLPLVGTTSPVGRSA
                     LAMLLFEDLAIVPIIFALGAMAPYAAAGGFGGLVNTLLLGAAAVAAMLVLGRLLLPRL
                     FAQAARTKSPELFLAASLLVVIVASLATTAVGLSPLMGAVLAGILIAETDYRSEVEVI
                     TAPFRGLALGIFLITVGMSVDLRLVAANWGSIALAVIGIVLLKALVTGLLLRLNGARR
                     AVAAEAGLIMASPSETTLIVLAAAAAAQLIRPETAAFWQIVTAVGLTITPLLAKLGQM
                     AARRVDVQSEDESPAIGRGDEAAVVIIGFGRVGRLVAEMLTAHGRRYLAVDSSIDTVA
                     DCRKEGIPIVYGDVARAELMDRLNLGHASALVLTMDDPVLSVRVTKKVRAWCPDLTIV
                     ARARDPAHAAELYRAGATDAVPETLESSLQLSEAVLVDLGMAMGPVIASIHEKRAELR
                     REIMELGHLTEEPVLERRRLRDALKAR"
     gene            3791..>4096
                     /locus_tag="VF547_00480"
     CDS             3791..>4096
                     /locus_tag="VF547_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8261329.1"
                     /translation="MDADLCIVGGGPAGLVAGLLFARAGVRVTVLEKHADFLRDFRGD
                     TVHPSTLRIFSELGLLDRLLARPHDKVRSVGGLIGGVAVELADLSAFDPRWNFIAMMP
                     "
BASE COUNT          623 a         1405 c         1536 g          532 t
ORIGIN      
        1 ccggcgaagc cgcccgcctc tggctgacgg agcgcgaggc ccgcctcagg gagaccgccg
       61 ccgctctcaa ggcctctccg gaggaagtcc ccgcccgcgt cgccgcgctg ctcgacgagc
      121 gccgtcggct cgagcgcgag ctcgccgagg cgaagaaggc tctggcgatg ggcggcggcc
      181 ggtcggccga ggcgcaggac ccggagcaga tcggcgggca cggcttcatc ggccaggtgc
      241 tcgagggcat ggatcccaag ggcctgcgcg gcctcgtcga cgaggcgaag cagcgcgtcg
      301 gctccggcgt cgtcgcgctg gtcgcggtca acgacggccg ggcgacggtg gcggtcgggg
      361 tcaccgacga tctcgccgcc cgccgcaacg ccgtcgatct ggtccgcaag gcggtcgagg
      421 cggtcggcgg ccagggcggg ggcgggcgcc ccgacatggc ccagggcggc ggcccggacg
      481 gagcgagggc cgacgaagcg ctcgacgcgg ttcggcgggc gctcagcgaa gagacggtcg
      541 ccgcgtaaat cccctctccc ctgcagggag aggattcaag ccgctcccgc ctccgccgcc
      601 ggcggctccc agccgaagat gctgcgcccg ccataagcgt tcgaaagccg ccgctcctcg
      661 gccatcaccg cctcgaacga gggccccgac acataggcct ccagccgccg cgcctgctcg
      721 tcgaggcggc ggatcgcgcc gagcttctcg tcctggccga gcttggcctg ctccacggcc
      781 gacttcagca cggagatggt ccggtcgtag accttggtcg gaaccgggaa gggatgccgg
      841 tccttgccgc catgggcgag cgagaagcgg gcggggtcgg cgaagcggca gggggcgccg
      901 tggatcacct cggcgaccat ggcgagcgag cggacggtgc gggcgccgac tcccgggacg
      961 agcagaagct cggggaaatc cttcgggccg gcctcggccg ccgcggcgag ggtgccgtgc
     1021 aggcggcgca ggatcacgtc ggtcggcctg acctcatggt ggccggggag ggcgaggtgg
     1081 ggctggagcg gctcggccgc cggggcgcgc tccagcgccg ccgcctcgcg ggcgaggcgg
     1141 tccggtccga gctcggcgag caggtcgagc tgcgcgcggc gcgaggcaac ggcgcggcgg
     1201 tcggcgaggt tgacgatctc gccctggcgg cggccttcga tcgcggcgtg gggcgaatcg
     1261 acgaagctgt cgaggccctt cgacagccag tggtagcggc gggcggtgcg gctggagccg
     1321 ttcatgccct gctggaccac cgcccatttg ccgtcgtcgg cgacgatgaa gccgtgcaga
     1381 tagaggtcga agccgtcctg gaccgcggcg ctgtcgacct tggcgacgag ccggctcgct
     1441 ctggcgaggg cggtgccgtc gaagccggct cgttcgccgg cgatggccag ctccgcgggc
     1501 gtctgccggc tgtgccgccc gcgcccgccg cacacctgca ggccgagctc gccggcgcgg
     1561 ggattgagcc cgcgcttgag cgctccgatg acgctggtgg tgattcccga cgaatgccag
     1621 tccatcccca tcacggcgcc gaacgactgg aaccagaagg ggtgcgcgag ccgggcgagc
     1681 aaggcgtccc ggccgtgatg gaggatgatg gcttccgcca tcaccgtgcc cagccgggtc
     1741 atccgctcgc ccagccaggc cggaacccgg ccgccgtgga gcgggaggtc ggcgctgcct
     1801 gagcgtctcg tcatgatcgg gtcctcagga acggaggagg aacgctagca ggagcggcct
     1861 gccgtgtcac ccgtgattgc gaggagcgac tcgacgaagc gatccggcag acctccgagg
     1921 ccgaaccgga ttgcttcgct tcgctcgcga tgacgggtta ccgcgccttc agcgcgtccc
     1981 gcagcctgcg ccgctcgagc accggctcct cggtgaggtg gccgagctcc atgatctccc
     2041 gacggagttc ggcgcgcttc tcgtggatcg aggcgatgac cgggcccatc gccatgccga
     2101 gatcgacgag caccgcttcc gacagctgga gcgaggattc gagagtctcc ggcaccgcgt
     2161 cggtggcgcc ggcgcggtag agctcggcgg cgtgggcggg gtcgcgggcg cgggcgacga
     2221 tggtgaggtc cgggcaccag gcgcgcacct tcttcgtcac ccggacgctc agcaccggat
     2281 cgtccatggt gaggacgagg gcgctggcat ggccgaggtt cagccggtcc atcaattcgg
     2341 cgcgggcgac gtcgccgtag acgatcggga ttccttcctt gcggcagtcg gcgaccgtgt
     2401 cgatgctcga gtcgaccgcc agatagcgcc ggccatgggc ggtcagcatc tcggcgacca
     2461 ggcgtccgac ccggccgaag ccgatgatca cgaccgcggc ctcgtcgccg cggccgatcg
     2521 ccggggactc gtcctccgac tgcacgtcca cccgtcgagc cgccatctgg ccgagcttgg
     2581 cgagcagcgg ggtgatggtg aggccgacgg cggtcacgat ctgccagaag gccgccgttt
     2641 ccgggcggat cagctgggcg gcggcggcgg cggcgagaac gatcaaggtg gtctcggaag
     2701 gggaggccat gatcagaccg gcttccgcgg ccaccgcccg gcgcgctccg ttgaggcgca
     2761 gcagcaggcc ggtgaccagc gccttgagaa gcacgatccc gatcacggcc agcgcgatcg
     2821 agccccaatt ggccgcgacg aggcgaaggt cgacgctcat tcccacggtg atcaggaaga
     2881 tgccgagggc gagcccgcgg aacggcgcgg tgatgacctc gacctcgctt cgataatcgg
     2941 tctcggcgat cagtatccca gccagcaccg cgcccatcag cggcgacagg ccgaccgcgg
     3001 tggtcgcgag gctggcgacg atcaccacca gcaggctggc ggccaggaac agctcggggc
     3061 tcttggtgcg tgccgcctgg gcgaacaggc gcggcagcag cagccgtccg agcaccagca
     3121 tcgcggccac cgccgccgcc ccgagcagca gcgtattgac cagcccgccg aacccgcccg
     3181 cggcggcgta aggcgccatc gcccccagtg cgaagatgat cgggacgatc gccaggtcct
     3241 cgaacagcag catcgcgagg gcggagcggc cgaccgggct ggtcgtgccg accaggggca
     3301 gaaccaaagc ggtcgatgaa agcgccagcg cgaggccgag cgcaaccgag ccgctccagc
     3361 tctgcccgaa ggcgtggagg gcgaggccga tcagaagcgc gcagacgagc agctcggccg
     3421 cgccgactcc gaacaccagc cgccgcatcg tccacagccg gcggaaggac agctcgaggc
     3481 cgatcgcgaa cagcagcagg acgatcccga attcggcgaa cggctccacc gcctcgggat
     3541 tggagatggt gatgtagttg agccagggcg cctgcccggt catcgcgccg aggcccgcgg
     3601 gaccgaccag caggccgacc aggatgaacc cgatcaccgg gctgatccgg aagcgggcga
     3661 aggcggggat gaccacgccg gcggcgccga ggacgacgag cgcgtcgctg aagccggagg
     3721 tttggaagtc ggcgtgcatc ttgcctttat gcaggaacgc gagccattgt cagcccgggg
     3781 gagggtcgaa atggacgctg atctctgcat cgtcggcggc gggcccgccg ggctggtcgc
     3841 aggccttctg ttcgcccggg ccggcgtgcg ggtgacggtg ctcgagaagc atgccgattt
     3901 cctgcgcgat ttccgaggcg acacggtcca tccctcgacc cttcggatct tctccgagct
     3961 gggcctgctg gaccggctgc tcgcccggcc gcacgacaag gtgcgcagcg tcggcggcct
     4021 gatcggcgga gtcgcggtcg agctcgccga cctgtccgcc ttcgatccgc gctggaactt
     4081 catcgcgatg atgccc
//
LOCUS       DASVQZ010000019         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_871616, whole genome shotgun sequence.
ACCESSION   DASVQZ010000019 DASVQZ010000000
VERSION     DASVQZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871616"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF547_00485"
     CDS             <1..819
                     /locus_tag="VF547_00485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8261330.1"
                     /translation="EVCEPNDALGRDEEAVGQLAKAILRDRRPLEMDRIPAGSRLIPA
                     LREALRGKGWVSVRPATPAPTIALESGWKNPSDRFNSGRRSDFRRAARRAAEMGELSF
                     EILSPSSGEFDALFDEAVAVELRSWKRDAGTAIAADQAKERCFRHFFRSACERGTFRI
                     AFLRIDGRAVAMQMALECLDRFWLFKIGFDEDYARCSPGTLLMLHTIGWAAQRELRAY
                     ELLGNVEPWIADFWTRESRDCVCLRAYPFNARGAAAFAADASAWLRKRLLPARR"
     gene            819..1655
                     /locus_tag="VF547_00490"
     CDS             819..1655
                     /locus_tag="VF547_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX8261331.1"
                     /translation="MLGPRGALRNARYAAPLYVERVASGPGPEAAARRCQILAGAGLR
                     ATVGYFQSSRAGAEEIVAANRAVAGLLADGGADVRLAVKAPPLAFDPARLRAVAEAAA
                     AAGLALTFDAHAAKDADRTLDAVAAFLPDFPRTGFALPARWRRSRADSDRFRDGPARI
                     RVVKGEWPDPDWSRPDIESNYLALIARLAGRSAPVAVATHDPDLAERALTLLIEAGTP
                     CELEQLRGLPARRTIAVAARLGVPVRIYVPFGPGWWPYALDKALERPYLLSWMIRDRL
                     GR"
     gene            1821..>2998
                     /locus_tag="VF547_00495"
     CDS             1821..>2998
                     /locus_tag="VF547_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX8261332.1"
                     /translation="MFFAAKSDPGTQQLTAEMAVTEVAVYRGPHYFSHTPMIRIQLDL
                     GRLEEWPTNRIAGFTEALLEMLPGVGRHGCSLRVRGGFEKRLRDGTWLGHVAEHVALE
                     LQTLAGSRATRGKTRSVKNRPGVYNVMFAYNEERVGLLAGRIALELVNSLLPQHLKGI
                     SGLDRIQALDGAFELDRRLDALKRTVRRTALGPSTRALVEEAQRRGIPVMRLDEKSLI
                     QLGHGRFQQKIRASITGRTSLVAADLAGDKAMTKTLLDESGVPVPRGVVVRDADEAVR
                     AARRLRYPLVVKPLDGNHGRGVTIGIENEDQLRFGFGEAQAQSKRGDVVVEQLFPGND
                     HRILVVGGRMIAVAERIPAQVVGDGISTIRRLVAEVNRDPRRGEGHENVMTRIRIDAH
                     V"
BASE COUNT          402 a         1096 c         1070 g          430 t
ORIGIN      
        1 gaggtgtgcg agcccaacga cgccctgggc cgggacgagg aggcggtcgg gcagctcgcg
       61 aaggcgatcc tgcgcgaccg gcggcccctg gagatggacc ggatcccggc gggctcccgg
      121 ctgatccccg cgctccgcga ggcgcttcgg ggcaagggct gggtctcggt ccggccggcc
      181 accccggcgc cgaccatcgc cctcgaatcc ggctggaaga acccgtccga ccgattcaat
      241 tccggccgtc gctcggactt ccggcgcgcc gcccggcgcg cggcggagat gggcgagctg
      301 tcgttcgaga tattgtcgcc ctcgtccggc gagttcgacg ccctgttcga cgaggcggtg
      361 gcggtcgagc tgcgcagctg gaagcgcgac gccggcaccg cgatcgccgc cgaccaggcc
      421 aaggagaggt gcttccgcca tttcttccgc tccgcctgcg agcggggcac gttccgaatc
      481 gccttcctcc ggatcgacgg gcgggcggtg gcgatgcaga tggcgctcga atgcctcgac
      541 cgcttctggc tgttcaagat cggcttcgac gaggattatg cgcgctgctc gcccggaacg
      601 ctgctgatgc tgcacacgat cggctgggcg gcgcagcgcg agcttcgcgc ctacgagctg
      661 ctcggcaacg tcgagccctg gatcgcggac ttctggaccc gcgagagccg cgactgcgtc
      721 tgccttcggg cctatccgtt caacgcgcgg ggcgccgccg ccttcgccgc ggacgcgtcc
      781 gcctggctgc gcaagcggct ccttccggcc cgccgctgat gctcggcccg cggggcgcgc
      841 ttcgcaacgc ccgttacgcg gcgccgctct atgtcgagcg ggtcgcttcc gggccggggc
      901 cggaggccgc cgcgcggcgc tgccaaatcc tcgccggcgc cgggctgcgg gcgacggtcg
      961 gatattttca gtcgagccgg gccggggcgg aggagatcgt cgccgccaac cgcgccgtcg
     1021 ccggcctgct ggccgacggc ggcgccgacg tccggctggc ggtgaaggcc cctcccctcg
     1081 cgttcgaccc ggcccggctg cgggcggtgg ccgaggccgc ggcggcggcg ggcctggcgc
     1141 tgacgttcga cgcccacgcc gccaaggatg cggaccgaac gctcgacgcg gtcgcggcct
     1201 tcctgcccga tttcccccgc accggcttcg ccctgcccgc gcgctggcgc cggagcaggg
     1261 ccgattccga ccggttccgc gacgggccgg cgcggatccg ggtggtgaag ggggaatggc
     1321 cggatcccga ctggtcgcgg cccgacatcg agtcgaacta tcttgccctg atcgcccggc
     1381 tggcgggccg ctcggccccg gtcgcggtcg ccacccacga tccggacctg gccgagcgcg
     1441 cgctcaccct gctgatcgag gccggaacgc cctgcgagct ggagcagctt cgaggcctgc
     1501 cggcgcgaag gacgattgcg gtcgcggcgc ggctcggagt tccggttcgg atctacgtcc
     1561 cgttcgggcc cggctggtgg ccctatgccc tcgataaggc gctcgagcgg ccctatctcc
     1621 tgtcctggat gatccgcgac cggctgggcc gctgaccgcc gccggcggcg cgcccgcacc
     1681 ccccgaactt gatccggatc agtgctttcg gcccggaact tgggcctgtt tccgcgggtt
     1741 tagctggggt catgacgcct ccgaaccagt ccgcgcccgt ggccgatgcg cggaccaagg
     1801 aaaccggctc ccaaaacggc atgttcttcg cggcgaagag cgatcccggc acccagcagc
     1861 tgaccgcgga gatggcggtc accgaggtcg ccgtctatcg cggcccccat tatttcagcc
     1921 acacgccgat gatccggatc cagctcgacc tcggccggct cgaggaatgg ccgacgaacc
     1981 ggatcgccgg cttcaccgag gcgctgctcg agatgctgcc cggcgtcggc cgccacggct
     2041 gctcgctgag ggtccgcggc ggattcgaga aacggctgcg cgacggcacc tggctcggcc
     2101 atgtcgccga gcatgtcgcg ctcgagctgc agacgctggc ggggtcgcgg gcgacccgcg
     2161 gcaagacccg ctcggtcaag aaccggcccg gcgtctacaa cgtcatgttc gcctataacg
     2221 aggagcgcgt cggcctgctg gccggccgga tcgcgctgga gctggtcaac tcgctgcttc
     2281 cgcagcacct gaagggcatc agcgggctcg accggatcca ggcgctggac ggcgccttcg
     2341 agctcgaccg ccggctcgac gcgctgaagc gcacggtgcg ccgcaccgct ctcggcccgt
     2401 cgacccgggc gctggtcgag gaagcgcaga ggcgcggaat cccggtcatg aggctcgacg
     2461 agaagagcct gatccagctc ggccacggcc gcttccagca gaagatccgc gcctcgatca
     2521 ccggccgcac ctcgctcgtc gcggcggacc tggccggcga caaggcgatg accaagaccc
     2581 tgctcgacga gagcggcgtc ccggtcccgc gcggagtcgt cgtgcgcgac gccgacgagg
     2641 cggtccgggc cgctcggcga ctgcgctacc cgctcgtcgt caagccgctc gacggcaatc
     2701 acggccgcgg cgtcacgatc ggaatcgaga acgaggacca gcttcggttc ggcttcggcg
     2761 aggcgcaggc ccagtccaag cgcggcgacg tcgtcgtcga gcagttgttc cccggcaacg
     2821 accatcgcat cctcgtcgtc ggcgggcgga tgatcgccgt cgccgagcgc atccccgccc
     2881 aggtcgtcgg cgacggcatc agcaccatcc gccggctggt cgccgaggtg aaccgcgacc
     2941 cgcggcgcgg cgaggggcat gagaacgtca tgacccggat ccggatcgac gcccatgt
//
LOCUS       DASVQZ010000020         7119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_583011, whole genome shotgun sequence.
ACCESSION   DASVQZ010000020 DASVQZ010000000
VERSION     DASVQZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583011"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..816
                     /locus_tag="VF547_00500"
     CDS             196..816
                     /locus_tag="VF547_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX8261333.1"
                     /translation="MRFRTLALAAAPVAAAAMLVPQAPVVAQAASPLSQVTAHLKAVN
                     TMVAGFRQTDRKGRSLTGTMTLKRPGKLRFQYQKGVPMLIVADGRKLNMIDYETKRVD
                     SWPIARSPLGVILNPNPDLSKIARVVRNDSQTLLVQVKDKPEFGTITLGFAKVPSAPA
                     GLLLQGWTVIDGQNNRTIVKLSGQRFNGAVADSAFTFERPVKGRKG"
     gene            1063..1857
                     /locus_tag="VF547_00505"
     CDS             1063..1857
                     /locus_tag="VF547_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8261334.1"
                     /translation="MAGTVKIASWNVNSVRARLDIVEKFLREEGPDVLCLQETKVCDA
                     DFPAALFGRLGYVHQIVCGQRMHHGVAILSRIPAREEARHDWQANGEARHVGVRLECG
                     VRLENVYVPAGGDTPDRAVNPKFGQKLDFLERMTRWSEACRCPTIMVGDFNVAPLECD
                     VWNHKQLLTVVSHTPVEVEALTRLQQSNDWVDLGRHFHPAPLRLHTWWSYRSLDWTKN
                     DRGRRLDHMWATPDVARLAVSHVVHEPCRNWTRPSDHVPLVTEFAI"
     gene            1854..2900
                     /gene="ribA"
                     /locus_tag="VF547_00510"
     CDS             1854..2900
                     /gene="ribA"
                     /locus_tag="VF547_00510"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX8261335.1"
                     /translation="MSGGRNVARAIDALRRGWPVRIEGIGFLAVETADETALAAFDAD
                     GPADLLISGNRAVTLKLANQRDAVPSGPVRIARTAMTLAEATAVADPALDLAHPLKGP
                     FRTTAVGCGRAGAAAVALARRAGLLPALFLGGAAAKDSVEVEAAEVEESERSPSLRIA
                     SRAKLPTRFAEKAEIVAFRSDDDGTEHVALVLGTPCGKRPLVRLHSECLTGDVLGSLK
                     CDCGPQLDSALDAIAASGWGILLYLRQEGRGIGLVNKLRAYALQDQGFDTVDANLRLG
                     FADDERDFTVAARMLEALAQDEVRLLTNNPRKVQALEAAGIRVAERVSLKAGSNAHNR
                     AYLETKRSRSGHAL"
     gene            complement(2918..3580)
                     /locus_tag="VF547_00515"
     CDS             complement(2918..3580)
                     /locus_tag="VF547_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8261336.1"
                     /translation="MRFAAIIFDFDGVIVDSEILANAILADALTELGHPTTTEQAVDR
                     YIGLHWADTCAAIEAEMGRALPADFKARTSEAFQLRLEEVTAVAGVADFLQALPPIPK
                     AVASSSPIRWLRGSLQRFGLAHHFGERLFSAAEHVPRGKPHPDIYLHAAAELGVEPGR
                     ALVIEDTPAGVTAARSAGMTVIGLCAGLHCGPGYGDRLRDAGAGHVAGAYREVLDIIV
                     SS"
     gene            complement(3577..4245)
                     /locus_tag="VF547_00520"
     CDS             complement(3577..4245)
                     /locus_tag="VF547_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX8261337.1"
                     /translation="MTSASPAQRLEQVRSAIARAARLAGRAPGEAALIAVSKTHGPEA
                     IAPLIEAGQRDFGENRVQEAQGKWPALKAGTPDLRLHLIGRLQSNKSEDAVELFDAIH
                     SVDRPSLVAALAAAMTKLGRRPECFLQVNIGDEPQKGGCAVADLPPLLEQSRAAGLPV
                     IGLMAIPPADVEPAPYFALLSKLARRHALPGLSMGMSADYETAVAIGATHVRVGTALF
                     GERR"
     gene            4268..4777
                     /locus_tag="VF547_00525"
     CDS             4268..4777
                     /locus_tag="VF547_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8261338.1"
                     /translation="MFARQPLPRLWLMTDERQGDDLWAALERLPRGAGVVFRHYGLAA
                     RERRKLFERVRRVAKRRRLLLLAGGKGPRGDGVHGGRRGFSASAHGLRELKTAERNGA
                     KLVFLSPAFPTRSHPGARALGPVRFGLIARQARIPVVALGGMEARKARRLPHIHGWAG
                     IDAFMSSPS"
     gene            complement(4926..5612)
                     /locus_tag="VF547_00530"
     CDS             complement(4926..5612)
                     /locus_tag="VF547_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8261339.1"
                     /translation="MGGAALVALSLILSPAIAANTQTGKKARPTASLRAGTFTPAVSD
                     PRLAAELARRGSQASSFRFTPAPTAVNRSQSIRVAIRARAATPAGALRRAADVSATPV
                     TAITPTVYNLGASIGWKRFALSGDVDSVKGGTLPGGRQAAEVGLSYSGNRFTARAEAG
                     VARADPTTPRIIADDESYTVGVGGSYRIARNLDVTGGVRYKVQRDRLEALADQRRDSQ
                     AVYVGTAFRF"
     gene            5880..6353
                     /locus_tag="VF547_00535"
     CDS             5880..6353
                     /locus_tag="VF547_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEX8261340.1"
                     /translation="MRRAAILALTAAGVFGLSACDFIREDINKSLGGGSPNRVREADL
                     AASQVTTIGVNSYLWRAALETLSFAPMAQVDSNGGVMVTDWYTNPSSPNERVKVTATI
                     LDRDLRADALRIAASRQVLQGGQWIDAPVAAATVQKLEEIILTRARDLRRNAIAS"
     gene            6368..>7119
                     /locus_tag="VF547_00540"
     CDS             6368..>7119
                     /locus_tag="VF547_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8261341.1"
                     /translation="MAARFNPLEADARWQKLWEERGTFHASGDSGRPRAYVLEMFPYP
                     SGRIHMGHVRNYTMGDVLARYRRMNGFDVLHPMGWDAFGMPAENAAMEKKVHPGEWTW
                     SNIDTMRAQLKRLGFALDWSRELATCDPSYYGHEQALFLDLYEAGLVYRKQSEVNWDP
                     VDMTVLANEQVIDGCGWRSGAPVERRKLSQWFLKITDFAEELLDGLGGLEHWPDKVRL
                     MQENWIGRSQGLRFRFELAEPVAGESGFEVFTT"
BASE COUNT         1103 a         2460 c         2475 g         1081 t
ORIGIN      
        1 agggagggcc ccgccgcgaa gcggtgggcg ggtgagggct tatgccccat ccccaggccc
       61 tcgccccggc gagccttgtg gcctgtcgct ctcccgggca gggaccgggg gtgagggaac
      121 tcgcgggttc agaggacatt caagcgcgga agggcagagc ccgcctcaaa atccactgat
      181 ggagaagatt cgatgatgcg tttcaggacg ctcgcccttg ccgccgcgcc ggtcgccgcg
      241 gcggccatgc tggtgccgca ggcgccggtc gtcgctcagg ccgcgtcgcc gctctcccag
      301 gtcaccgcgc acctcaaggc cgtcaacacg atggtcgcgg gctttcggca gaccgaccgc
      361 aagggccgtt cgctgaccgg cacgatgacc ctgaagcggc cgggcaagct gcgcttccag
      421 tatcagaagg gcgtgccgat gctgatcgtc gcggacggtc gcaagctcaa catgatcgat
      481 tacgagacca agcgggtcga cagctggccg atcgcccgct ccccgctcgg cgtcatcctc
      541 aaccccaatc cggacctcag caagatcgcc agggtcgtgc gcaacgactc ccagaccctc
      601 ctcgtccagg tcaaggacaa gcccgaattc ggcaccatca cgctcggctt cgccaaggtg
      661 ccgagcgcgc cggcgggcct gctcctgcag ggctggacgg tgatcgacgg ccagaacaac
      721 cggactatcg tgaaactcag cggccagcgc ttcaacggcg ccgtcgccga cagcgccttc
      781 acgttcgagc ggcccgtgaa gggcaggaag ggctaggccg gccgcgccgc cgttcggcgc
      841 cgtctccgcc cgccgggccg cggccattca tccgcgcgac aggaatgtga cactaataca
      901 tcttttgcga ttgcgggtgg tcgctgtccg ggttttcccc ctgttgcccg gacacgccgc
      961 cgcgatcgag cctcgcgtag accacgcttg gttggccctc gctccacgcc cccggagcgg
     1021 gggcctttgc gttgggggcc tcaaccgcta gagccttcag ccatggctgg aaccgtgaag
     1081 atcgcgtcgt ggaacgtcaa ttccgtccgc gcccgcctcg acatcgtcga gaagttcctg
     1141 cgcgaggagg ggcccgacgt tctgtgcctg caggagacga aggtctgcga cgcggatttc
     1201 ccggcggcct tgttcgggcg cctcggctac gtccaccaga tcgtctgcgg ccagcggatg
     1261 caccacggcg tggcgatcct cagccgaatt ccggcgcgcg aggaggcccg gcacgactgg
     1321 caggcgaacg gcgaggcccg ccacgtcggc gttcggctcg aatgcggcgt ccgcctcgag
     1381 aatgtctacg tcccggccgg cggcgacacg ccggaccggg cggtgaaccc gaagttcggc
     1441 cagaagctcg acttcctcga gcggatgacg cgctggtcgg aagcgtgccg gtgcccgacg
     1501 atcatggtcg gcgacttcaa cgtcgcccca ctcgaatgcg acgtctggaa ccacaagcag
     1561 cttctcacgg tggtcagcca cacgccggtc gaggtcgagg ccctgaccag gctccagcag
     1621 agcaacgatt gggtcgatct cggccgccat ttccatcccg cccccctgcg cctccacacc
     1681 tggtggagct accgctcgct ggactggacc aagaacgacc gcgggcgccg gctcgaccat
     1741 atgtgggcca ctccggacgt cgcccggctg gcggtgagcc atgtggtcca cgagccctgc
     1801 cgcaactgga ctcgtccgtc cgaccacgtc ccgttggtca cggaattcgc gatttgagcg
     1861 gcgggcgcaa cgtcgcccgc gcgatcgatg cgctgcggcg cggctggccg gttcggatcg
     1921 agggcatcgg cttcctcgcc gtcgagaccg ccgacgagac ggcgctggcc gcgttcgacg
     1981 ccgacgggcc ggcggacctg ctcatttcgg gcaatcgcgc cgtgaccctc aagctcgcca
     2041 accagcgcga cgccgtgccg agcgggcccg tccggatcgc gcggacggcg atgaccctcg
     2101 ccgaggccac cgccgtggcc gaccccgcgc tcgacctcgc ccatcccctc aagggcccgt
     2161 tccggacgac cgccgtcggc tgcggccgag ccggcgccgc ggccgtcgcg ctcgcgcgcc
     2221 gggcgggcct gctgccggcc ctgtttctcg gcggcgcggc cgcgaaggac tcggtcgagg
     2281 tcgaggccgc cgaggtcgag gagtcggagc gctcgccgtc gcttcgaatc gcctcgcgcg
     2341 cgaagcttcc gacgcggttc gcggagaagg ccgagatcgt cgccttccgc tccgacgacg
     2401 acggcaccga gcatgtcgcc ctcgtcctgg gcaccccttg cggaaagcgc ccgctggtca
     2461 ggctccacag cgaatgcctg accggggacg tgctcggctc gctcaaatgc gactgcgggc
     2521 cgcagctcga ttcggccctc gatgcgatcg ccgcatcggg atgggggata ctcctctatc
     2581 tccgccagga agggcgaggc atcggcctcg tcaacaagct gcgcgcctac gccctccagg
     2641 accagggctt cgacacggtc gacgccaacc tacggctcgg cttcgcggat gacgagcgcg
     2701 acttcaccgt cgccgcgcga atgctcgagg cgctcgcaca ggatgaggtg cgcctgctga
     2761 ccaacaatcc ccgcaaggtc caggcgctgg aggccgccgg cataagggtc gccgaacgtg
     2821 tgtcgctcaa ggcggggagc aatgcccaca accgggccta tctggaaacc aagcgctcgc
     2881 gcagcggcca cgcgctttga cgccatgacg tctggcatca cgaagataca atgatatcaa
     2941 gtacttccct gtaggcgccg gcaacgtggc cggccccagc atcgcggagc cggtcgccgt
     3001 agccgggacc gcagtggagg cccgcgcaga gcccgatcac ggtcattccg gccgatcggg
     3061 ccgcggtcac gccggccggc gtgtcctcga tgaccagcgc ccgcccgggc tccacgccga
     3121 gctcggccgc cgcgtgcagg tagatgtcgg gatggggctt gccgcgcggc acatgctcgg
     3181 cggcgctgaa caggcgctcg ccgaaatgat gggccaggcc gaaccgttgg agcgatccgc
     3241 gcagccaccg gatcgggctc gacgaggcga ccgccttcgg gatcggcggc agtgcttgaa
     3301 ggaagtccgc gactccggcc acggcggtca cctcctccag cctgagctgg aaagcctcgc
     3361 tggtccgcgc cttgaaatcc gccgggagcg ccctccccat ctccgcctcg atcgccgcgc
     3421 aggtgtcggc ccagtgcagg ccgatatagc ggtcgaccgc ctgctcggtc gtcgtcggat
     3481 ggccgagctc ggtcaaggca tcggcgagaa tggcattggc caggatctcg ctgtcgacga
     3541 tcacgccgtc gaaatcgaag attatcgccg cgaacctcag cgtctttctc cgaacaaggc
     3601 ggtgccgacg cggacgtggg tcgcgccgat cgccaccgcc gtctcgtaat cggccgacat
     3661 gcccatgctg aggccgggca gggcgtggcg gcgggccagc ttcgagagca gggcgaaata
     3721 gggggccggc tcgacgtccg ccggtgggat ggccatcagc ccgatgacgg ggaggccggc
     3781 ggcgcggctc tgctccagca acggcggcag atcggccacg gcgcagccgc ccttctgcgg
     3841 ctcgtcgccg atattgacct ggaggaagca ttccggccgg cggccgagct tcgtcatcgc
     3901 cgccgccagc gccgccacca gggaggggcg gtccaccgaa tggatggcgt cgaacagctc
     3961 gacggcgtcc tccgacttgt tcgactgcag ccggccgatg agatggagcc ggaggtccgg
     4021 cgtgccagcc ttcagcgcgg gccacttgcc ctgcgcctcc tggacgcggt tctctccgaa
     4081 gtcgcgctgg cccgcctcga tcagcggcgc gatcgcttcg ggcccgtggg tcttggaaac
     4141 ggcgatcagg gccgcttccc ccggcgctcg gccggcgagc cgcgcggcgc gcgcgatcgc
     4201 gctccggacc tgctcgagcc gctgggcggg tgatgcgctg gtcatggcgg ctgctatagg
     4261 aagcgccatg ttcgcccgcc agcctctgcc gcgcctctgg ctgatgacgg acgagcgcca
     4321 gggggacgat ttgtgggcgg cgctggagcg gctgccgaga ggagcggggg tcgtcttccg
     4381 tcattacggc ctcgcggcgc gagagcggcg gaagctgttc gagcgggtgc gccgggtggc
     4441 gaaacgccgg cggcttctcc tgctcgccgg cggcaaggga ccgcggggcg acggggtgca
     4501 tggcggacgc agggggttca gcgcttcggc ccacggcctg cgcgagctga agacggctga
     4561 gcgcaacggc gcgaagctgg tgttcctctc gccggccttt ccgacccgct cccacccggg
     4621 ggcccgggcg ctgggaccgg tgcgattcgg cctgatcgct cgacaggcga ggattccggt
     4681 ggtcgcgctg ggcgggatgg aggcgcgaaa ggctcggcgg ctgcctcata tccacggctg
     4741 ggcagggatc gacgctttca tgtcctcccc atcttaagat ggggaggggg gaccctgcga
     4801 agcagggtgg agcggtaact ggcgaggaat tctggaggtt cgagcgagat ccgctgcaat
     4861 ctgcgcctta cccctccacc acagttcgtg tggtccccct ccccatgctc cgcatgggaa
     4921 ggatcttaga agcggaaggc ggtgccgacg tagacggcct ggctgtcgcg ccgctgatcg
     4981 gccagcgctt cgaggcggtc gcgctggacc ttgtagcgga ctccgccggt gacatcgaga
     5041 ttgcgggcga tccggtagga gccgccgact ccgaccgtat agctctcgtc gtcggcgatg
     5101 atccgcggcg tggtgggatc ggcccgggcg acgccggcct cggcgcgagc ggtgaagcgg
     5161 ttgcccgaat agctgaggcc gacctccgcc gcctggcggc cgccggggag cgtcccgccc
     5221 ttcacgctgt cgacgtcgcc ggacaaggcg aatcgcttcc agccgatcga ggcgccgaga
     5281 ttgtagacgg tgggcgtgat cgcggtgacc ggggtcgcgg aaacgtcggc ggcgcggcgc
     5341 agcgctcccg ccggagtcgc ggcgcgggcg cggatggcga cccggatcga ctggctgcgg
     5401 ttgaccgcgg tcggcgccgg cgtgaagcgg aagctgctgg cctgcgagcc gcggcgcgca
     5461 agctcggcgg cgaggcgcgg atccgagacg gcgggggtga aggtgccggc gcgcagcgag
     5521 gcggtcggcc gcgccttctt gcccgtctgg gtgttcgccg cgatcgccgg agacaggatg
     5581 agggacagcg cgacgagcgc cgcacctccc actgcaaccg gtcctgcgag cttcataacc
     5641 aaggctggcg actcccgtac agatcgggat gtagtacgtc caaacgactc aaaccagcga
     5701 agcggcccgg gcggacacga atcgttccgg catgttgcat cttttccaca gcccggcctt
     5761 gacatgcgca cagcagaggc cccgcgcgcg gcgtcggttc ctagagagac cgcttgccgc
     5821 ccgcgcggcg cgttctataa cggcgctcga cccctgtagt ttcaaggaca cattctttca
     5881 tgcgacgcgc tgcaattctg gccctgaccg ccgccggcgt tttcggcctc tcggcctgcg
     5941 atttcatccg cgaggacatc aacaagtccc tcggcggcgg ctcgccaaat cgggtcaggg
     6001 aggcggatct ggccgcctcc caggtgacga ccatcggagt caattcctac ctctggcgcg
     6061 ccgcgctcga aaccctgtcc ttcgcgccca tggcccaggt cgattcgaac ggcggggtga
     6121 tggtcaccga ctggtacacc aatcccagct cgccgaacga gcgggtcaag gtcacggcca
     6181 cgatcctcga ccgggacctg cgggccgacg cgctgcgcat cgccgcctcc cggcaggtgc
     6241 tgcagggcgg acaatggatc gacgccccgg tcgccgccgc caccgtccag aagctcgagg
     6301 agatcatcct cacccgcgcc cgcgaccttc gccggaacgc catcgccagc tgaggagcag
     6361 ccctgagatg gcggcgcgtt tcaacccgct cgaggccgac gcgcgctggc agaagctgtg
     6421 ggaggagagg ggtaccttcc acgcctccgg cgacagcggc cgccctcgcg cctatgtcct
     6481 cgagatgttc ccctatccct cggggcgcat ccatatgggc catgttcgca actatacgat
     6541 gggcgacgtc ctcgcgcgct atcggcggat gaacggcttc gacgtgctcc acccgatggg
     6601 ctgggacgcg ttcggaatgc cggccgagaa tgcggcgatg gagaagaagg tccatcccgg
     6661 cgaatggacc tggtcgaaca tcgacacgat gcgcgcgcag ctcaagcgcc tcggcttcgc
     6721 gctcgactgg agccgcgagc tggccacctg cgacccctcc tattacggcc acgagcaggc
     6781 gctgttcctc gatctctacg aagcggggct ggtctatcgc aagcagagcg aggtcaattg
     6841 ggacccggtc gacatgaccg tgctggccaa cgagcaggtc atcgacgggt gcggctggcg
     6901 ctcgggcgcc ccggtcgagc ggcgtaagct cagccagtgg ttcctgaaga tcaccgactt
     6961 tgccgaagag ctgctcgacg gcctcggcgg actcgagcat tggcccgaca aggtgcggct
     7021 gatgcaggag aactggatcg gcaggagcca ggggctgcgc ttccggttcg agctggccga
     7081 gccggtagcg ggcgaatcgg gcttcgaagt cttcaccac
//
LOCUS       DASVQZ010000021         7523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_679264, whole genome shotgun sequence.
ACCESSION   DASVQZ010000021 DASVQZ010000000
VERSION     DASVQZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679264"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VF547_00545"
     CDS             <1..467
                     /locus_tag="VF547_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8261342.1"
                     /translation="PVMRWLGGLRTAQEHREIILGRIIPWQEERGFTFWVVERKADDS
                     LLGFCGLKLASDPGSPVEGQVEIGWRLREDSWGQGYAREAASASLDFAFGRLGAPNVV
                     ALTVAGNAASRRLMERLGMTRRPDLDYEGPDWADGPVIVYRIEREEWTGRSS"
     gene            446..997
                     /locus_tag="VF547_00550"
     CDS             446..997
                     /locus_tag="VF547_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX8261343.1"
                     /translation="MDRAQLIGFGGKTPRIDAGAFVAPGAVLIGDVEIGPEASIWYNC
                     VLRGDVNRIRVGARSNIQDGSVVHVDSPRPGKEAGLPTIIGEDVLIGHMAMVHGCILH
                     DRAFVGLGAIVMDGCEIESGAMLAAGGLLAPGRRIPAGQLWAGRPAKYVRDLSEEELA
                     GQQAGVAHYVALARLHAAAIRGG"
     gene            complement(984..1490)
                     /locus_tag="VF547_00555"
     CDS             complement(984..1490)
                     /locus_tag="VF547_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261344.1"
                     /translation="MAGLKAPLAAFLTLLLAAACAAPGPYPSLAPRAAEKAYAGEDER
                     QPTPQADDPALAAEIDRLAAAARAGAADFDAALPAAQSAAAAAGAAGSDSWIEAQQDL
                     SRLEAARARTTAALADLDRLAVERASAGTLGEGDRERLRAATAEIQALADAQADRLRR
                     LGESLIRL"
     gene            1571..1647
                     /locus_tag="VF547_00560"
     tRNA            1571..1647
                     /locus_tag="VF547_00560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1605..1607,aa:Arg,seq:acg)
     gene            complement(1657..2214)
                     /locus_tag="VF547_00565"
     CDS             complement(1657..2214)
                     /locus_tag="VF547_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8261345.1"
                     /translation="MARAVRRVVTGHDEEGRSIVLEDGPPARVATLGGESGTTFHEVW
                     NTRRTPAPIDRASGEPHEPGLSLLPPRGGTRIRVLDIPPDDGTVAALPREAVKALFEA
                     IGAGHALTEDPPHPLMHRTETVDYGIVLEGELVLILDGGETIVRAGDIVVQRGTSHAW
                     ANRSNANARIAFVLIDGRFEEALQG"
     gene            2417..2881
                     /locus_tag="VF547_00570"
     CDS             2417..2881
                     /locus_tag="VF547_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8261346.1"
                     /translation="MRLPRPLIRTVFTFLQGLRRLLWLVTGPGRDGVHAVPLTPAGKV
                     VLVRLTYAPGWRLPGGGRKKSETAVDGMLRELSEEIGLVAHGAIERLDDIHPGGLPGD
                     RSALFLVSDVEYRPRQSLEVDEVAEFDRDELPEDLSRSTRETLDALAGRRGR"
     gene            complement(2878..3063)
                     /locus_tag="VF547_00575"
     CDS             complement(2878..3063)
                     /locus_tag="VF547_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEX8261347.1"
                     /translation="MPAKSKAQQKAAGAALSAKRGDTPKSALKGASKQMASSMTEKQL
                     EEFASTKRKGKPEHVGD"
     gene            3149..3865
                     /locus_tag="VF547_00580"
     CDS             3149..3865
                     /locus_tag="VF547_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8261348.1"
                     /translation="MDEIEWWEFGTAREMAEQAAGDIGFVVESAIEAHKGARLALPGG
                     TVGDLIYAQLARSTAIDWPSVTIMPTDDRLVPLGDSLSNYRKLEGFFAAKRANLVSLV
                     DEAALEDRQEAGRLADARLSLVGWPLDLACLGMGADGHTAGLFPGPDLERAVAGPRER
                     RAVGVRPDPMPDEAPVERVTLTAPALASARAIMIVIAGSEKRRVLEQAIKEGPLSAAP
                     IGRLLAAVDAPIDIFWSAEG"
     gene            complement(3980..5233)
                     /locus_tag="VF547_00585"
     CDS             complement(3980..5233)
                     /locus_tag="VF547_00585"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEX8261349.1"
                     /translation="MPLAEINFDGIIGPSHNYSGLSLGNIASATNKGLVSYPRAAALQ
                     GLGKMRHNLRLGLPQGLLPPHRRPDRDWLAQLGTAVEDVPASLRPAAFSASAMWAANA
                     ATVSPEPDTGDGRCHLTIANLRTMAHRSHEWPETLAQLRLAFADETRFAVHPPVPATF
                     GDEGAANHMRLCLAHGEPGVEVFVYGTRGGAFPVRQHVEASQAVARLHGLDPDRALFV
                     AQSEEAVAAGAFHNDVVAVANETVLFAHEQAFEDRPAFYAELRRRLPEVEIVEVPAAS
                     VSLGDAIASYLFNAQLVTLPAGGMALILPEEARETPSVWAWLQAMVAGNGPIRQLFVV
                     DVRQSMANGGGPACLRLRVVAEPEAVDPRFIADESKLDRLAALVEAEWPERIEPEDLL
                     DPALWARMEGARAALLGALDLEELV"
     gene            complement(5233..6252)
                     /locus_tag="VF547_00590"
     CDS             complement(5233..6252)
                     /locus_tag="VF547_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEX8261350.1"
                     /translation="MSFRIRPARNEDFQALYEMAKLTGGGFTNLPPDRGALVEKLIRS
                     QKSFATDASQPGDELFVLMLENAESGQVRGTCQMFGMVGVETPFYSYRIGTLTQTSKA
                     LGKTFRAQMLSLSTDLEGSSEVGGLFLHPGERAGGLGMLLARSRYLFIKMHRERFGRR
                     VLAELRGVIDEAGGSPFWDAIAGRFFGMNFQEADEFNAAHGTQFIADLMPKTPIYTAM
                     LPESARSVMGVPHPSGRAAMKMLEAEGFQFDCYIDIFDGGPTMVAATDQIRTVRESRD
                     LVLAEVADSVDGPNMMLARGRLADFVACCGKVRIDAEERATIDRATAALLGIEPGESF
                     LAMGR"
     gene            complement(6249..7463)
                     /locus_tag="VF547_00595"
     CDS             complement(6249..7463)
                     /locus_tag="VF547_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX8261351.1"
                     /translation="MRGLSSDEAAAMERSAGEPMLAQVEAWAAVNSGSRNLDGLARVA
                     ALLADAFAGLPGALTLRDPAPVESVAADGSVRSIEHGRNLHLAVRPEAPVQLLFTGHM
                     DTVFAADHPFQDVVPREPDGVLGGPGVADMKGGLAVLLAALKSAEASPLAERFGYEVV
                     INSDEEVGSPGSAPLIGEAARGKRAALTFEPSALPDGTLAGARPGSGNFSLVVAGRSA
                     HAGRNPEDGRNALLAAAELALRLADCAGPGLNVNPAKIDGGGPSNVVPDHAVLRMNMR
                     PVTLADQARAQSALEDAVADVAARREVRITVHGGWGRPPKPLDPKAEKLFALVRACGA
                     DLGQTIGWRASGGVCDGNNIAACGVPVVDTMGVRGGAIHSPDEYLIVDSLAERAQLSA
                     LTILRLARGEGL"
BASE COUNT         1045 a         2645 c         2738 g         1095 t
ORIGIN      
        1 agccggtgat gcggtggctg ggcgggctga ggacggcgca ggagcatcgc gagatcatcc
       61 tcggccggat cattccctgg caggaggagc gcggcttcac cttctgggtg gtggagcgca
      121 aggccgacga ctcgcttctc ggcttctgcg gcctcaagct cgcaagcgac ccgggcagcc
      181 cggtcgaggg ccaggtcgag atcggctggc ggctgcgcga ggactcctgg gggcagggct
      241 acgcccgcga agcggcgagc gcgtcgctcg acttcgcgtt cggccggctc ggcgcgccga
      301 acgtcgtcgc gctcaccgtc gccggcaatg ctgcgagccg gcggctgatg gagcgcctcg
      361 gcatgacgcg gcggcccgat ctcgattatg agggccccga ctgggcggac ggaccggtca
      421 tcgtctatcg gatcgagagg gaagaatgga cagggcgcag ctcataggct tcggcgggaa
      481 gacgccgcgg atcgacgccg gggccttcgt cgcgcccggg gcggtgctga tcggcgacgt
      541 cgagatcggg cccgaagcca gcatctggta caattgcgtg ctgaggggcg acgtcaaccg
      601 gatccgggtc ggcgcccgaa gcaacatcca ggacggcagc gtcgtccatg tcgattcgcc
      661 ccgtcccggc aaggaagcgg gcttgccgac gatcatcggc gaggacgtgc tgatcggcca
      721 tatggcgatg gtccatggct gcatcctcca cgaccgcgcc ttcgtcggcc tgggggcgat
      781 cgtgatggac ggctgcgaga tcgagagcgg cgcaatgctc gccgccggcg gcctgctcgc
      841 gcccgggcgg cgcattccgg ccggccagct ctgggcgggt cggccggcca aatatgtccg
      901 cgacctcagc gaggaggagc tggccgggca gcaggcggga gtcgcccatt atgtcgcgct
      961 ggcgaggctc cacgccgccg cgatcagagg cggatgaggc tctccccgag ccgccggagg
     1021 cgatcggcct gggcgtcggc cagggcctgg atttcggcgg tggccgcgcg cagcctttcg
     1081 cggtcgcctt cgccgagcgt tccggcgctg gcccgctcca ccgccagccg gtcgaggtcc
     1141 gcgagcgccg ccgtcgtccg agcccgcgcc gcttccagcc gggagagatc ctgctgggcc
     1201 tctatccagg aatcgcttcc ggctgcgccg gcggcggccg cggccgactg cgcggccgga
     1261 agcgcggcgt cgaaatcggc ggcgccggcc cgcgcggcgg cggcgagtcg gtcgatctcg
     1321 gcggcaagcg ccggatcgtc ggcctgcggc gtcggctgcc gctcgtcctc tcccgcatag
     1381 gccttctccg cggcgcgcgg cgcgagggac gggtaggggc ccggcgccgc gcaggcggcg
     1441 gccagaagga gcgtaaggaa agccgcgagg ggcgctttga ggccggccat ggctcctcct
     1501 aggagccgga ggcggcacgg gcaacggggt tgcgcggcgc cgcccttgcc cctatgtccc
     1561 cgtctcccgc gcgcccatag ctcagctgga tagagcacca gactacgaat ctgggggccg
     1621 gaggttcgaa tccttctggg cgcgccaatt ttcccttcag ccttgcagcg cctcctcgaa
     1681 gcgtccgtcg atcaggacga aggcgatgcg ggcgttcgcg ttcgatcggt tggcccaggc
     1741 gtggctggtg ccgcgctgga cgacgatgtc gcccgcgcgg acgatcgtct cgcccccgtc
     1801 gaggatgagg acgagctcgc cttcgaggac gatgccatag tccaccgtct cggtgcggtg
     1861 catgagcgga tggggcggat cctcggtcag cgcgtggccg gcgccgatcg cctcgaacag
     1921 cgccttcacc gcctcgcgcg gcagggcggc gacggtgccg tcgtcgggcg ggatgtcgag
     1981 cacccggatg cgcgtcccgc cgcgcggcgg cagcagcgag agtccgggtt cgtggggctc
     2041 gcccgaggcg cggtcgatcg gggccggggt ccggcgcgtg ttccacactt cgtggaaggt
     2101 ggtcccgctc tcgccgccca gcgtcgccac gcgggccggc ggcccgtcct cgaggacgat
     2161 cgagcgcccc tcctcgtcat gccccgtcac cacccggcgg actgcccgcg ccatcgtctc
     2221 ctccgtcctt cttgagtccc ggtggtggcc gcaaagcggc cgcggttgca agcgctgcgc
     2281 gaccgcccta cctgcggccg gcccgatgaa gctcttccca tccgccgccc tgaccgcgct
     2341 tcccgccggc cccgccttcg cccggcagga tcgaagcggg aggatgatct tcctgcgcgt
     2401 tgcgatagac tagggcatga ggctgccccg ccccctgatc cggaccgtct tcaccttcct
     2461 ccagggcctt cgccggctgc tctggctggt gaccggtccc ggccgcgacg gggtccacgc
     2521 ggtgccgctg acgccggccg gcaaggtggt cctggtgcgc ctcacctacg cccccggctg
     2581 gcggctgccc ggcggcggcc ggaagaagag cgagacggcc gtcgacggca tgctgcgcga
     2641 gctgagcgag gagatagggc tcgtcgccca cggcgcgatc gagcggctcg acgacatcca
     2701 ccccggcggc ctgcccggcg accgctccgc tctgttcctg gtcagcgacg tcgagtaccg
     2761 gccccggcag agcctggagg tcgacgaggt cgccgagttc gaccgggacg agcttcccga
     2821 ggatctctcc cgttcgaccc gggagacgct cgacgccctc gccggccgcc gggggcgcta
     2881 gtcgccgacg tgctcgggct tgcccttgcg cttggtcgac gcgaactcct cgagctgctt
     2941 ctcggtcatc gaggaggcca tttgcttcga cgcgcccttc agggcgcttt tcggagtgtc
     3001 gccgcgcttg gcggacaaag cggcgcccgc ggccttctgc tgcgctttcg atttggcagg
     3061 catggtttgt ctccctaagg cgatcgccgg ctggaacggg ccgcgcccgc cgagggttcc
     3121 tggtccccgc tgaggagcga cgcagaatat ggacgagatc gaatggtggg agttcggcac
     3181 cgcgcgggag atggcggagc aggcggcggg ggacatcggt ttcgtcgtcg agagcgcgat
     3241 cgaggcgcat aagggcgccc gcctcgccct tccgggcggc acggtcggcg atctgatcta
     3301 cgcgcagctg gccagatcga cggcgatcga ctggccgagc gtgacgatca tgccgaccga
     3361 cgaccggctg gtgccgctcg gcgattcgct cagcaactac cgaaagctcg aaggcttctt
     3421 cgcggcgaag cgggcgaacc tcgtctcgct ggtcgacgag gcggcgctcg aagaccgcca
     3481 ggaggcgggc cgcctcgccg acgcccggct gtcgctggtc ggctggccgc tcgacctcgc
     3541 ctgcctcggc atgggcgccg acggccacac cgccggcctc tttcccggcc cggacctcga
     3601 gcgcgccgtg gctgggccgc gcgagcgccg cgccgtcggc gtccgccccg atccgatgcc
     3661 cgacgaggcg ccggtcgagc gggtcacgct caccgcgccg gccctggcct cggcgcgcgc
     3721 gatcatgatc gtcatcgccg gctccgagaa acgccgcgtg ctcgagcagg cgatcaagga
     3781 aggaccgctc tccgccgccc cgatcggccg actgctcgcc gccgtcgacg ccccgatcga
     3841 catcttctgg agcgcggagg gctaggtcct tcgatacggg gcttcgactt cgctcagccc
     3901 ctactcagga tgagcgacta tttttccgct catgctgagt aggcattgag cgaagtcgaa
     3961 atgccgtatc gaagcacgat cagaccagct cctccaggtc cagggcgccg agcagcgccg
     4021 ccctggcgcc ctccatccgc gcccacaggg ccgggtccag caggtcctcg ggctcgatcc
     4081 gctcgggcca ttcggcttcg accagcgcgg cgagccgatc gagcttcgat tcgtccgcga
     4141 tgaaccgcgg gtccaccgcc tccggctcgg ccacgacccg cagcctcagg catgccggcc
     4201 cgccgccatt ggccatcgac tggcgcacgt cgacgacgaa cagctggcgg atcggcccgt
     4261 tgccggcgac catggcctgg agccaggccc agaccgacgg cgtctcgcgc gcctcctcgg
     4321 ggaggatcag ggccatgccc cccgcgggca gggtcaccag ctgggcgttg aacaggtagc
     4381 tggcgatcgc gtcgcccagg ctgaccgagg ccgccggaac ctcgacgatc tcgacctcgg
     4441 gcagacggcg cctgagctca gcatagaaag ccgggcggtc ctcgaaggcc tgctcgtgcg
     4501 cgaacaggac ggtctcgttg gcgaccgcga cgacgtcgtt gtggaaggcg ccggcggcga
     4561 cggcctcctc cgactgggcg acgaacagcg ctcggtcggg atcgaggccg tggaggcggg
     4621 cgacggcctg gctcgcctcg acatgctggc ggaccgggaa ggcgccgccc ctcgtgccgt
     4681 agacgaacac ctcgacgccc ggctcgccgt gggcgaggca gagccgcata tgattggcgg
     4741 cgccttcgtc gccgaaggtg gcggggacgg gcgggtggac ggcgaagcgc gtctcgtcgg
     4801 cgaaggcgag tcggagctgg gcgagcgtct cgggccattc gtggcttcgg tgcgccatgg
     4861 tccgcagatt ggcgatcgtg aggtggcagc ggccgtcccc ggtatccggc tcgggcgaga
     4921 cggtggcggc gttggcggcc cacatcgccg aggcggagaa ggcggcgggg cgcagcgagg
     4981 ccggaacgtc ctcgacggcg gtgccgagct gggcgagcca gtcccggtcc ggacggcggt
     5041 ggggcggcag cagcccctgc ggcaggccga gccgcaggtt gtgccgcatc ttgccgaggc
     5101 cctgaagcgc cgccgcgcgc ggataggaga cgagcccctt gttggtcgcc gaggcgatat
     5161 tgcccaggct gaggcccgaa taattgtggc tcgggccgat gattccgtcg aagttgatct
     5221 cggcgagcgg catcaacgcc ccatggcgag gaaggattcg ccgggctcga tgccgagcag
     5281 ggccgcggtg gcgcgatcga tcgtcgcccg ctcctccgca tcgatgcgca ccttgccgca
     5341 gcaggcgacg aaatcggcga gccggccgcg ggccagcatc atgttcgggc cgtcgaccga
     5401 atcggcgacc tcggccagca ccaggtcgcg cgattcgcgg acggtgcgga tctggtcggt
     5461 ggcggcgacc atcgtcgggc cgccgtcgaa aatgtcgata tagcagtcga actggaagcc
     5521 ttccgcctcc agcatcttca tcgcggcccg gccggaagga tggggaacgc ccatcaccga
     5581 gcgggcgctt tcgggcagca tcgccgtgta gatcggggtc ttgggcatca ggtcggcgat
     5641 gaactgggtg ccgtgggcgg cgttgaattc gtcggcctcc tggaagttca tcccgaagaa
     5701 gcggccggcg atcgcgtccc agaaaggcga gccgcccgcc tcgtcgatga ctccgcgaag
     5761 ctcggcgagg acccggcggc cgaagcgctc gcggtgcatc ttgatgaaca gatagcggct
     5821 gcgcgccagc agcattccaa gcccgcccgc ccgctcgccc gggtggagga acagcccgcc
     5881 gacctccgag gagccttcga gatcggtgct gaggctgagc atctgcgctc ggaaggtctt
     5941 gccgagcgcc ttggaggtct gcgtcagggt cccgatccgg tagctgtaga agggggtttc
     6001 cacgcccacc atcccgaaca tctggcaggt gccccgcacc tggccgctct cggcgttctc
     6061 gagcatcagg acgaacagct cgtcgccggg ctggctcgcg tcggtggcga agctcttctg
     6121 cgagcggatc agcttttcaa ccagcgcgcc ccggtccggc ggcaggttgg tgaagccgcc
     6181 gccggtcagc ttggccatct cgtagagcgc ctggaaatcc tcgttgcgcg cgggcctgat
     6241 ccggaagctc acaaaccctc tccccgcgcc agccgaagga tggtgagcgc cgacaattgc
     6301 gcccgctcgg cgaggctgtc gacgatcaga tattcgtccg gcgaatggat cgcgccgccg
     6361 cgcacgccca tcgtgtcgac caccggcacg ccgcaggcgg cgatgttgtt gccgtcgcac
     6421 acgccgcccg aagcgcgcca gccgatcgtc tggccgagat cggcgccgca ggccctgacg
     6481 agggcgaaca gcttttcggc cttcgggtcg agcggcttgg gcgggcgtcc ccagccgccg
     6541 tggacggtta ttcgaacctc gcgccgagcg gcgacgtcgg ccacggcgtc ctcgagcgcc
     6601 gactgggccc gcgcctggtc ggcgagggtc accgggcgca tgttcatgcg caggaccgca
     6661 tggtcgggaa cgacgttgga aggaccgccg ccgtcgatct tcgccgggtt gacgttgagc
     6721 ccgggcccgg cgcaatcggc cagccgaagc gccagctcgg ccgccgccag aagcgcgttg
     6781 cggccgtctt ccgggttgcg cccggcatgc gcgctccggc ccgcgacgac gagcgagaaa
     6841 ttgccgcttc ccggccgagc gcccgccagc gtgccgtcgg gcagcgccga gggctcgaag
     6901 gtcagggcgg cgcgcttgcc gcgggccgct tccccgatca gcggcgccga gccgggcgag
     6961 ccgacctcct cgtcggaatt gatcaccacc tcgtagccga accgctcggc gagcggcgag
     7021 gcttccgcgc tcttgagggc cgccagcagg accgcgaggc cgcccttcat gtccgccacg
     7081 ccggggccgc cgagcacccc gtccggttcc cgcgggacga cgtcctggaa cggatggtcg
     7141 gcggcgaaca ccgtgtccat gtgcccggtg aagagcaatt ggaccggcgc ctccgggcgc
     7201 accgcgagat ggaggttgcg gccatgctcg atcgagcgga ccgagccgtc ggcggcgacc
     7261 gactcgaccg gcgcggggtc gcgcagggtc agcgcccccg gcaagccggc gaaggcgtcc
     7321 gcgagcaggg ccgccacacg cgccagaccg tcaaggttgc gcgagccgct gttgaccgcc
     7381 gcccaggcct cgacctgcgc cagcatcggc tcaccggccg agcgctccat cgccgccgct
     7441 tcgtcgctcg aaaggccccg catgaggccg cctgtagccc acgataggcg cctaggccac
     7501 tcccgaggcc ggggggcgcc gcc
//
LOCUS       DASVQZ010000022         7219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_5528,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000022 DASVQZ010000000
VERSION     DASVQZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5528"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF547_00600"
     CDS             <1..186
                     /locus_tag="VF547_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5'-phosphate-dependent protein"
                     /protein_id="HEX8261352.1"
                     /translation="AFAARHLRIVVEPGGAVALAALLAGKAGEVTERTVVVVSGGNVD
                     PALYAAILAGQAAEAAR"
     gene            complement(162..419)
                     /locus_tag="VF547_00605"
     CDS             complement(162..419)
                     /locus_tag="VF547_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261353.1"
                     /translation="MPRREEDWPLDEAFERIEESILPSLSLTIDGMIEAATLARPGHD
                     AELFAAELRTLGLQLEALTRQVEAVSRGGIEAAGYRAASAA"
     gene            487..672
                     /locus_tag="VF547_00610"
     CDS             487..672
                     /locus_tag="VF547_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261354.1"
                     /translation="MAQARSMYTIAVTALSLAVIASFALVGGGIYLLAKRKEGRQGLL
                     MLIAAAVLFGNVLMWTL"
     gene            660..1379
                     /locus_tag="VF547_00615"
     CDS             660..1379
                     /locus_tag="VF547_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261355.1"
                     /translation="MDAVNPLLLVAAMLSQAPAQAPVDERPLESWTCTVGERLDDNVG
                     GMLQMLVDLDGTRREMSYYVNWSSQPGHMAEQRMSWIWIPLDAQRLWKPDEIEFSMKG
                     ERTDEKGSVIFQSPKYGRIFRSADDLAKSLRPTFDMTWIKIGEAYLIAQLWDGWPWTA
                     EHSDRNGVSLGSQAILLPGPEGVQAMFTRLEARLDQDAAQPAAKCQANYGPSQRELEE
                     SQIHRGSPIRRPDLTPATPPR"
     gene            1384..2163
                     /locus_tag="VF547_00620"
     CDS             1384..2163
                     /locus_tag="VF547_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX8261356.1"
                     /translation="MTARIFDFDRAIVREPGDTVVDGLRTGPEAPRHARVLAEHRAYV
                     AALELAGVAVEILPALPDHPDSVFVEDPAFVLTQAAVLLRPGAPSRFAEPAALEPVLR
                     RHFPDVASVDEGFIDGGDILVLPDEILVGLSARTDERGARRFREIVRDLGRNVRVVEP
                     PPGLLHLKTGCALIDERTVIAIPAMASLFPGFEVVVTPEGEEPSANLIRVNDRILMGD
                     CFPRTAALLAERGLDLVAIPCAEIAKIDAGLSCMSLRWRSA"
     gene            2231..3598
                     /locus_tag="VF547_00625"
     CDS             2231..3598
                     /locus_tag="VF547_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8261357.1"
                     /translation="MQSTNPAIDALLSAFSASADPRLLLSALDLAEQDSERRAVIAAS
                     LGAPGAELDAPARERLARAALDMGDALDAVALCGEDPALLAVRVDALLVAGRAVEAET
                     VYRAAVAADPLLEDPAIDSRLEEAKAAEPAMASNIIGFAQAARGLRGADRSEREEEAA
                     ARALFAEPGGAVRFADVGGLDEVKKQISRRIVTPFRKPSLFAKYRRKAGGGVLLFGPP
                     GCGKTLLARATAGECEARFVNVPVVDVVDKYIGEAERKLAAIFADARRDTPTVLFFDE
                     LEALAGSRSGNANQSHVSLVSTFLAEMDGFAHNNEGVLILAATNMPWGVDSAFRRPGR
                     FDRVQFVPPPDRAARAEILRMQLSGRPVAPDCDVDSIAARTAGYSGADLENLVNTAVD
                     LAIEEALASGEEAPVAARHLVEAFSEVRPTTLEWLTTARNYAKYSNAGGQYDDVAAFL
                     KANGL"
     gene            3595..3975
                     /locus_tag="VF547_00630"
     CDS             3595..3975
                     /locus_tag="VF547_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261358.1"
                     /translation="MSDALRYRLIDEPREGPLQRLALPPLLVFIVATFFTPWGFVLIA
                     LNAVALGGPARNREIALAGLALLIYFASRIALGLAVRAGLVELRPAVYVFEAAVGVAL
                     VCLAFAYVSQHRAAELRQYLRRQA"
     gene            3975..4904
                     /locus_tag="VF547_00635"
     CDS             3975..4904
                     /locus_tag="VF547_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8261359.1"
                     /translation="MQPYGLAIHRAEALLAAGNWAAAIEPLRDALTAAPQEAYPHLLL
                     SRALRQQGRLAGARYEADRAVEHAPLWHVAHLELAQVLLAQQHGKAALEAADRAVEIA
                     PGDGFAHLVRARILRAQGRRAEAQASLAEARSLEPNFAPIMAEEGYAALEQGRMDVVE
                     SSGREILTLNPDDPDGLILLGHARLTRGDVEEALRLALSALANAPTDLDALFLLASVK
                     MRKSLAGGLFWRWNRLLVKLGEAKAIFFVVGIFVLYRWALLASHDLGLPAAVETVLMV
                     VYLGFVIYTLSAGTIVRRMVEKEVARVRLDPRF"
     gene            complement(4962..5618)
                     /locus_tag="VF547_00640"
     CDS             complement(4962..5618)
                     /locus_tag="VF547_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261360.1"
                     /translation="MPEVIFPGPEGRLEGRFSPGPRPRAPVALILHPHPQGGGTMNNA
                     IVQALYKTFVRRGFATLRFNFRGVGKSQGVFDNGIGELSDAASALDWVQQVHPEAETT
                     WIAGFSFGAWIGMQLLMRRPEVRGFISIAPPANMYDFTFLAPCPASGIIIQGEGDEVV
                     TPNAVQKLVDKLRTQKHITIHHSTIPQANHFFVNEMPQLMKAVDQYLDFRLDPSCPIR
                     "
     gene            5718..6782
                     /locus_tag="VF547_00645"
     CDS             5718..6782
                     /locus_tag="VF547_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8261361.1"
                     /translation="MPDRIYLDHASTTPILPQAAAAMADACRSWANPSSPHAEGRAAR
                     AQLEDARRRIAAAMGSASTLIFTSGATEAISLVFERARAKDRIVSAVEHPAVLRSALG
                     ARQIAVRRDGTLDLDDLDRALESAQAPLVAVQSVNNETGVIHPIDQVLARVKAAGGLL
                     LTDCAQSAGKLPLPEADFIVLAAHKFGGPPGIGALLVRDTATLEAIGGQEGGYRPGTA
                     AVPSIMGFAAAAEADHAWYEEAERLRARLDEGIVAAGGEIAAADAPRIATIACYRMPG
                     VPGAAQMMQLDLAGIAVTAGSACASGSLKTSHVLTAMGWPEEEAREVIRVSFGPQTSG
                     SDVNALLGHWTAMYGKRRAA"
     gene            6779..>7219
                     /locus_tag="VF547_00650"
     CDS             6779..>7219
                     /locus_tag="VF547_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8261362.1"
                     /translation="MREPVYLDYQATTPLAPEAAAAMRPWLEDRFANPHSSHRLGREA
                     AAVVEVARGRVLEALQIPLPARSEGASPEESLPAETDPPLTPPCIQGGELYFTSGATE
                     AINWALKGAAARLPESRRRIVTLATEHAAVLDTVEYLASLGFDSE"
BASE COUNT         1048 a         2599 c         2452 g         1120 t
ORIGIN      
        1 gcgtttgccg ctcggcacct ccgaatcgtc gtcgagccgg gcggcgccgt cgccctcgcg
       61 gcgctgctcg ccggcaaggc gggcgaggtg acggagcgaa cggtggtcgt ggtgtcgggg
      121 gggaacgtgg atccggccct ctacgcggcc atcctcgcgg gtcaggcggc cgaggcagcc
      181 cggtagccgg cagcctcgat gccgccgcgg gagacggctt cgacctgccg ggtcagcgcc
      241 tccagctgca ggcccagcgt ccgaagctcg gcggcgaaga gctctgcgtc atggccgggg
      301 cgggcgagcg tggccgcctc gatcatccca tcgatcgtca gcgacaggct cggcaggatc
      361 gactcctcga tccgctcgaa agcctcgtcc agcggccaat cctcttccct ccggggcatg
      421 ggcgaaggct acgcctcgcc ctttaaccat tcgtaaaaag acgcctctgt tttcccgtcc
      481 cttcggatgg cacaagcacg gtcgatgtac acgatcgccg tcaccgccct gtcgctcgca
      541 gtcatcgcct ccttcgccct cgtcggcggc gggatctacc tgctggcgaa gcgaaaggag
      601 ggcaggcagg gcctgttgat gctgatcgcc gccgccgtgc tgttcggcaa cgtgctgatg
      661 tggacgctgt gaacccgctg ctcctcgtcg ccgcgatgct ctcgcaggcg cccgcccagg
      721 cacccgtgga cgaacggccg cttgagagct ggacctgcac ggtcggcgaa cggctcgatg
      781 acaatgtcgg cggaatgctg cagatgctgg tcgacctgga cggcactcgc cgcgagatgt
      841 cctattacgt aaactggtcg tcgcagccgg gccatatggc cgagcaaagg atgagctgga
      901 tctggatccc gctcgatgcc caacggctct ggaagccgga cgagatcgag ttttcgatga
      961 agggcgagcg gacggacgag aagggatcgg tgatcttcca gtcgccgaaa tacggccgga
     1021 tcttccgttc ggcggacgac ctggccaagt cgctccggcc gaccttcgac atgacctgga
     1081 tcaagatcgg cgaagcctat ctgatcgcgc agctgtggga cgggtggccg tggacggccg
     1141 agcattcgga ccgcaacggc gtcagcctcg gctcgcaggc gatccttctg cccggcccgg
     1201 aaggcgtgca ggcgatgttc acgcgcctgg aggcccgcct cgaccaggac gcggcgcaac
     1261 cggccgcgaa gtgccaggcc aattacggcc ccagccagcg ggagctcgag gaatcccaga
     1321 tccatcgggg cagtccgatc cgccgtccgg acctcacgcc cgcgactccg ccgcgctgac
     1381 ctcgtgaccg cacggatctt cgatttcgac cgggccatcg tccgcgagcc cggcgacacc
     1441 gtcgtcgacg gccttcgaac cggccccgag gcgccccgcc atgcccgagt cctcgccgag
     1501 catcgcgcct atgtcgccgc cctcgaattg gccggagtcg cggtcgagat cctgccggcc
     1561 cttcccgatc atcccgattc ggtcttcgtc gaggacccgg ccttcgtcct cacccaagcc
     1621 gccgtcctgc tccgccccgg cgcccccagc cgattcgccg agccggcggc gctggagccc
     1681 gtgcttcgcc gccatttccc cgacgtcgct tcggtcgacg agggcttcat cgacggcggc
     1741 gacatcctcg tccttcccga cgagatactg gtcgggctgt cggcgcggac cgacgaacgc
     1801 ggggcgcggc gcttccggga gatcgtccgg gacctcggcc ggaacgtccg cgtcgtcgag
     1861 ccgccgccgg gcctgctcca cctcaagacc ggctgcgccc tgatcgacga gcggaccgtg
     1921 atcgcgattc cggcgatggc ctcgctgttt ccgggcttcg aggtggtggt cactcccgag
     1981 ggggaggagc cgtccgccaa tctgatccga gtcaacgatc gaatcctgat gggggactgc
     2041 ttcccgcgca ccgccgccct cctcgccgaa cgcggcctcg acctggtggc gattccctgc
     2101 gccgagatcg ccaagatcga cgccggcctc tcctgcatgt cgctgcgctg gcgctcggcc
     2161 tgaccggccc gtcttcaacc gttgccttgg tgatgcggcc cactatgctt gcgcctcgaa
     2221 ggggaattcc gtgcagtcga ccaatccggc catagacgcg ctcttgagcg ccttttcggc
     2281 gagcgccgac ccgcggctgc tcctctccgc gctcgatctc gcggagcagg attcggagcg
     2341 gcgggcggtg atcgccgctt cgctcggggc gcccggggcg gagctggacg cgccggcgcg
     2401 cgagcggctc gcccgggcgg cgctggacat gggcgacgcg ctcgacgccg tcgccttgtg
     2461 cggcgaggac cccgcgctcc ttgcagtcag ggtcgacgcc ctgctcgtgg ccggccgcgc
     2521 cgtcgaggcc gagaccgtct accgggccgc ggtcgccgcc gatcccctgc tcgaggatcc
     2581 cgccatcgac tcgaggctcg aggaagccaa ggcggccgag ccggcgatgg cgtccaacat
     2641 catcggcttc gcccaggcgg cgcgcggcct gcgcggcgcg gaccggagcg agcgggagga
     2701 ggaggcggcc gccagggcgc tgttcgcaga gccgggcggc gccgtccgct tcgccgacgt
     2761 gggcggcctc gacgaggtca agaaacagat ttcccgccgg atcgtcactc cgttcaggaa
     2821 gccctcgctc ttcgccaaat accgccgcaa ggccggcggc ggggtgcttc tgttcggtcc
     2881 ccccggctgc ggcaagacgc ttctcgcccg ggccaccgcc ggcgagtgcg aggcccggtt
     2941 cgtcaacgtc cccgtcgtcg acgtggtcga caaatatatc ggggaagcgg agcgcaagct
     3001 ggcggccatc ttcgccgacg cgcgccgcga cacgccgacc gtgctcttct tcgacgagct
     3061 ggaggcgctc gccggctcgc gcagcggcaa cgccaaccag agccatgtca gcctcgtctc
     3121 gacgttcctg gccgagatgg acggcttcgc ccacaacaac gaaggcgtcc tgatcctcgc
     3181 ggcgaccaac atgccctggg gcgtcgactc cgccttccgg cgccccggcc gcttcgaccg
     3241 ggtccagttc gtgccaccgc cggatcgcgc cgcgcgcgcc gagatcctcc ggatgcagct
     3301 gtcgggacgg ccggtcgctc ccgattgcga cgtcgactcg atcgccgcga ggaccgccgg
     3361 ctattcgggc gccgacctcg agaacctggt caataccgcg gtcgatctcg ccatcgagga
     3421 ggcgctcgcc tccggggagg aggctcccgt cgccgcccgc catctggtcg aggccttcag
     3481 cgaggtccgc cccaccacgc tcgaatggct cacgacggcc cggaactacg ccaaatattc
     3541 caatgccggc ggccagtatg acgacgtcgc cgccttcctg aaggcgaacg ggctttgagc
     3601 gacgcgcttc gctaccggct gatcgacgag ccgcgcgaag gccccctcca gcggctcgcg
     3661 cttccgccgc tgctcgtctt catcgtcgcc accttcttca ccccctgggg gttcgtgctc
     3721 atcgccctca atgcggtcgc gctcggcggg ccggcgcgca accgcgagat cgcgctcgcc
     3781 ggacttgctc tcctgattta tttcgcgtcc cggatcgcgc tcggcctggc ggtccgggcc
     3841 ggcctggtcg aattgcgccc ggcagtctac gtcttcgagg cggcggttgg agtcgcgctg
     3901 gtctgcctgg ccttcgccta tgtgtcgcaa caccgggccg ccgagctgcg ccagtatctt
     3961 cggcggcagg cctagtgcag ccgtacggcc tcgccatcca ccgtgccgaa gcgctgctcg
     4021 cggccggcaa ttgggcggcg gcgatagagc cgcttcgcga cgccctgacc gccgccccgc
     4081 aggaagccta tccgcacctg ctgctttcgc gcgcgctccg ccagcagggc cggctcgccg
     4141 gcgcacgcta cgaggcggac cgggcggtcg agcatgcgcc gctctggcat gtcgcgcatc
     4201 tcgagcttgc ccaggtcctg ctcgcgcagc agcacggcaa ggccgcgctc gaggccgcgg
     4261 accgagcggt cgagatcgcg cccggagacg gcttcgccca tctcgtccgc gctaggatcc
     4321 ttcgagccca gggccgccgt gccgaggccc aggccagcct cgccgaggcg cgctcgctcg
     4381 agccgaactt cgcgccgatc atggccgagg agggctatgc ggcgctcgag cagggaagaa
     4441 tggacgtcgt cgagtcgtca ggccgggaga tattgaccct caatcccgac gatccggacg
     4501 gcctcatcct gctcggccac gcccggctca cccgcggcga cgtcgaggag gcgctgcgcc
     4561 tcgcgctcag cgcgctcgcc aacgctccga ccgacctcga cgccctgttc ctgctggcct
     4621 cggtcaagat gcgcaagagc ctcgcgggcg gcctgttctg gcgctggaac cggctgctcg
     4681 tgaagctcgg cgaggcgaag gcgatcttct tcgtcgtcgg gattttcgtg ctctaccgct
     4741 gggcgctcct cgccagccat gacctcgggc ttccggcggc cgtcgagacg gtgctgatgg
     4801 tcgtctatct cggcttcgtc atctatactc tgtcggcggg gacgatcgtg agaaggatgg
     4861 tcgaaaagga ggtggcgcga gtcaggctcg acccacgctt ctgaccccac cccgccggga
     4921 aacgcggatg gacggccctc gaccccgatc gccgggaaac gtcagcggat cgggcagctc
     4981 ggatccagcc tgaaatcgag atactggtcc accgccttca tcagctgcgg catctcgttg
     5041 acgaagaaat ggttcgcctg cggtatggtc gaatgatgga tggtgatgtg cttctgggtc
     5101 ctgagcttgt cgacaagctt ctgcaccgcg ttcggggtca ccacctcgtc gccctcgccc
     5161 tggatgatga tgcccgaggc ggggcagggg gcgaggaagg tgaagtcgta catgttggcc
     5221 ggaggcgcga tcgagatgaa gccgcgcacc tcgggccgcc gcatcagcag ctgcatcccg
     5281 atccaggcgc cgaagctgaa gccggcgatc cacgtcgtct ccgcctcggg gtggacctgc
     5341 tggacccagt cgagcgccga ggcggcgtcg gacaattcgc cgatgccgtt gtcgaacacg
     5401 ccctggctct tgccgactcc gcggaagttg aaccgcaacg tcgcgaagcc gcgcctgacg
     5461 aaggtcttgt agagcgcctg gacgatggcg ttgttcatcg tcccgccgcc ctgcggatgg
     5521 gggtggagga tcagcgcgac cggcgctcgc ggccgggggc cggggctgaa acggccttcg
     5581 agacggcctt ccggaccggg aaagatgact tcgggcattg cacctgcgac ggctcaaggg
     5641 aaagagcggg gctgcccccg cgcgcatggg gctctatata ggcgtgcgcg cccgccgcgc
     5701 aatttgaaag ggaagacgtg cccgatcgca tctatctcga ccacgcctcg acgaccccga
     5761 tcctgccgca ggcggcggcg gcgatggcgg atgcgtgccg gagctgggcc aacccctcct
     5821 cgccccatgc cgaggggcgg gcggcgcggg cgcagctcga ggatgcgagg cgccggatcg
     5881 ccgccgcaat gggctcggcc tcgaccctca tcttcacctc cggcgccacc gaggcgatct
     5941 cgctggtctt cgagcgcgcg cgggcgaagg accggatcgt ctcggcggtc gagcatccgg
     6001 ccgtgctgcg cagcgctctg ggcgcgcggc agatcgccgt ccgtcgcgac ggcacgctcg
     6061 acctcgacga cctcgaccgc gccctcgaat cggcgcaggc gccgctggtc gcggtgcagt
     6121 cggtcaacaa cgagaccggg gtcatccatc cgatcgacca ggtgctggcg agggttaagg
     6181 ccgcgggcgg gctgctcctc accgactgcg cccagagcgc gggcaagctg ccgcttcccg
     6241 aagccgactt catcgtcctt gcggcgcaca agttcggggg gccgcccggc atcggcgccc
     6301 tgctggtgcg cgacaccgcg acgctggagg cgatcggcgg gcaggagggc ggctatcgcc
     6361 cgggcaccgc cgccgtgccc tcgatcatgg gcttcgccgc cgcggctgag gcagatcacg
     6421 cctggtacga ggaggccgag cgcctccgcg cccgtctcga cgagggcatc gtcgccgcgg
     6481 gaggggagat cgccgcggcc gatgctccgc gcatcgccac catcgcctgc taccgcatgc
     6541 ccggcgttcc cggcgccgcc cagatgatgc agctcgatct ggccggaatc gccgtcaccg
     6601 cgggcagcgc ctgcgcctcg ggcagcctca agaccagcca cgtcctcacc gccatgggct
     6661 ggcccgagga ggaggcgcgc gaggtgatcc gagtcagctt cggcccgcag acgagcggga
     6721 gcgacgtgaa cgccttgctc ggccactgga cggcgatgta cgggaaacgc cgggcagcgt
     6781 gagagagccc gtctatctcg actaccaggc gaccaccccg ctcgccccgg aagcggccgc
     6841 ggcgatgcgg ccctggctgg aagaccgttt cgccaacccc cacagcagcc accgcctcgg
     6901 ccgcgaagcg gccgcggtcg tggaagtggc ccgggggcgg gtgctggagg ccctccaaat
     6961 cccgctccct gctcgcagcg agggtgcgtc gcctgaagag tcgttgccgg cggagaccga
     7021 cccacccctg acccctccct gtattcaggg aggggaattg tacttcacct ccggcgccac
     7081 cgaggcgatc aattgggcgc tgaaaggcgc ggccgcccgc ctccccgaga gccgccgccg
     7141 catagtcacc ctcgccaccg agcatgccgc cgtgctcgac accgtcgaat atctcgcgag
     7201 cctgggcttc gactccgaa
//
LOCUS       DASVQZ010000023         3580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_679777, whole genome shotgun sequence.
ACCESSION   DASVQZ010000023 DASVQZ010000000
VERSION     DASVQZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679777"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF547_00655"
     CDS             complement(<1..556)
                     /locus_tag="VF547_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261363.1"
                     /translation="MTDTPPPAAKPKLGFAQLWNISFGFFGIQIGFALQNANMSRIFQ
                     SLGTSLDDLPALWVAAPLTGLLVQPVIGHMSDRTWLGRLGRRRPYFLAGAILAALSLF
                     LMPLAPVLLFAAVMLWVLDASLNVSMEPFRAFVGDMLNKDQHSAGYAVQTAFIGAGAV
                     VGSIFPWALEQIGVSNEAGPGGIPD"
     gene            complement(553..2076)
                     /locus_tag="VF547_00660"
     CDS             complement(553..2076)
                     /locus_tag="VF547_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8261364.1"
                     /translation="MVSRIVIVGGGTAGWMAAAALARFLGPRTDIRLVESEEIGTVGV
                     GEATIPQLRLFNAGLGIDEDAFVRETAGTFKLGVEFVGWGDPDQRYIHAFGTIGRDLG
                     LIPFHQYWLRHRAEGGTADLWDFSASAQAARHNLFGRWEERPERLPSGLAHAFQFDAG
                     LYAAYLRRYAEARGVQRTEGKVKDVTLRSEDGFVEAVTLESGERIGGDLFIDCSGFRG
                     LIIEQALRTGYEDWSKWLPCDRALAVPCASGGEFTPYTRATARAAGWQWRIPLQHRTG
                     NGHVYCSAFMSDDEAARVLLANLDGPALADPRPLRFVAGKRRKAWNRNCVALGLAGGF
                     MEPLESTSIHLVQSGIARLLQLFPARGFMPEEADEYNRQTDVEWLSVRDFLTFHYWAN
                     GRDGEFWRCCRAIEVPESLERRVALFRANGRIFRNSEELFAEVGWLQVMIGQGVVPAG
                     YHPLADQLSSDQLDEFLALGRKHVAHIATQLPDHAEFIARNCAIPQPRAAASAGARL"
     gene            complement(2150..>3580)
                     /locus_tag="VF547_00665"
     CDS             complement(2150..>3580)
                     /locus_tag="VF547_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8261365.1"
                     /translation="PQGWGGGVLPQVGYYNNRIVDDELWQFRAQLERELESGFLSGIK
                     AGFNYTLRDKSLRPDEALIRLAGGALEAPIPTQFLLEPTNLNYLGLGPMVSYDPRELL
                     AAGNVYSLASNSANPDVVAKAYTVNEKLLTGYVQADIRAELGSATLTGNVGVQAVRTD
                     QSSNGNVIVGGVITPAEAGVKFWDILPSLNLSLRLESDWVFRLALAREMQRPRLDQMR
                     IAVSYGLDKAEGIIKGSGGNPFLRPTRANAIDFNVEKYFGRSGYVALQLFYKDLVNFI
                     YDPRETDFDFTGYPLSAADEALATTRIGRLSTAINAEGGHMYGAEAAGTLPFSVFADA
                     LDGFGITGGLSYTKTRIRETATSNPEAIPGYSKWVANGTAFFERAGFSARGSVRHRTS
                     FLGELSGFGGNRTRRQALRETIVDAQIGYDFQPGSRLEGLSLYLQGQNLTDERFATIF
                     DPANPLTVIDYQIYGRRFLAGFNYKF"
BASE COUNT          578 a         1201 c         1244 g          557 t
ORIGIN      
        1 tgtccgggat gccgccgggt cctgcttcgt tggacacgcc gatctgctcc agcgcccagg
       61 ggaagatcga tccgaccacc gcgcccgcgc cgatgaaggc ggtctgcacc gcatagccgg
      121 cgctgtgctg gtccttgttc agcatgtcgc ccacgaaggc gcggaacggc tccatcgaga
      181 cgttcaatga ggcatcgagc acccacagca tcaccgccgc gaagaggagc acgggcgcga
      241 gcggcatcag gaagagcgag agcgcggcga ggatggcccc ggcgaggaaa tagggccggc
      301 ggcggccaag gcggccgagc caggtgcggt cgctcatatg accgatcacc ggctggacga
      361 gcagcccggt gagcggcgcc gccacccaca gcgccggtag atcgtcgagg ctggtgccga
      421 gcgattggaa gatccggctc atattcgcgt tctgcagcgc gaagccgatc tggatcccga
      481 agaagccgaa gctgatgttc cagagctggg cgaagccgag cttcggcttg gcggcgggag
      541 gcggcgtgtc ggtcaaaggc gtgctcctgc gctggcggcc gcgcgcggct gcgggatcgc
      601 gcagttgcgg gcgatgaatt cggcgtggtc gggaagctgg gtggcgatat gggcgacatg
      661 cttccgcccc aacgccagga actcgtcgag ctgatcgctc gagagctggt cggcgagcgg
      721 atggtagccg gccgggacga cgccttggcc gatcatcacc tgcagccagc cgacctcggc
      781 gaacagctcc tcgctgttgc ggaagatgcg gccgttggcg cggaacaggg cgactcgccg
      841 ctcgaggctc tccggcacct cgatcgcgcg gcagcagcgc cagaattcgc cgtcccggcc
      901 attcgcccag taatggaagg tgaggaagtc ccgcacggac agccattcga cgtcggtctg
      961 gcggttatat tcgtccgcct cctcgggcat gaagccgcgc gccgggaaga gctgcagcag
     1021 tcgcgcgatg ccggactgga cgaggtggat gctggtcgat tccagcggct ccatgaagcc
     1081 gccggcgagc ccgagcgcga cgcagttgcg gttccaggcc ttgcgcctct tgcccgccac
     1141 gaagcgcagc gggcgcggat cggcgagggc gggcccgtcg agattggcga gcaggacccg
     1201 cgccgcctcg tcgtcgctca tgaaagcgct gcaatagaca tggccgttgc cggtgcggtg
     1261 ctggagcggg atccgccact gccaccccgc cgcccgcgcc gtcgcccggg tatagggcgt
     1321 gaactccccg ccggaagcgc aggggacggc gagcgcccgg tcgcacggca gccatttcga
     1381 ccagtcctca tagccggtgc ggagcgcctg ctcgatgatt aggccgcgaa agcccgagca
     1441 gtcgatgaag aggtcgccgc cgatccgctc gccgctctcg agcgtcacgg cctctacgaa
     1501 gccgtcctcg ctgcgcagag tcacgtcctt caccttgccc tcggtgcgct ggacgccccg
     1561 cgcctcggcg tagcgccgca gataggcggc gtagaggccg gcgtcgaact ggaacgcgtg
     1621 ggcaagcccg ctcggcaacc gctcgggccg ctcctcccac cggccgaaga ggttgtgccg
     1681 cgccgcctgc gccgaggcgg agaagtccca caggtcggcg gtcccgccct ccgcccgatg
     1741 ccgaagccaa tattggtgga aagggatcag cccgaggtcc cgcccgatcg ttccgaaggc
     1801 gtggatgtag cgctggtccg gatcgcccca gccgacgaac tcgaccccca gcttgaacgt
     1861 ccccgccgtc tcgcggacga acgcgtcctc gtcgatcccg agcccggcgt tgaacaggcg
     1921 cagctggggg atcgtcgcct cgccgactcc caccgttccg atctcctcgg actcgaccag
     1981 ccggatgtcc gttcggggcc cgaggaatcg cgccagcgcc gccgcggcca tccagccggc
     2041 ggtgccgccg ccgacgatca cgatccggct caccatggcg tgggcctaac atgaaacgag
     2101 ccgcgcctcc cccccgaaga cgcggcccgc cccatcgctc gcggcggcgt cagaacttgt
     2161 agttgaagcc ggcgaggaac cgccgcccat agatctggta gtcgatcacg gtgagcgggt
     2221 tcgccggatc gaagatggtg gcgaaccttt cgtcggtcag attctggccc tgcaggtaga
     2281 gcgacaggcc ctccagcctc gatcccggct ggaaatcgta gccgatctgg gcatcgacga
     2341 tcgtctcgcg cagcgcctgc cggcgggtcc ggttgcctcc gaagccggaa agctcgccga
     2401 ggaagctggt ccggtggcgg acgctgccgc gggcggagaa gccggccctt tcgaagaagg
     2461 cggtgccgtt cgccacccat ttcgaatagc ccgggatcgc ctccggattg ctcgtcgccg
     2521 tctctcggat cctggtcttg gtataggaga ggcctccggt gattccgaag ccgtcgagcg
     2581 cgtccgcgaa gacgctgaac ggcagcgttc ccgccgcctc cgcgccgtac atatggccgc
     2641 cctccgcgtt gatcgcggtg ctgagccgcc cgatgcgggt cgtggcaagg gcctcgtcgg
     2701 cggcgctgag cgggtagccg gtgaaatcga agtccgtctc gcgcgggtcg tagatgaagt
     2761 tgaccaggtc cttgtagaag agctgcaggg cgacatagcc gctccggccg aaatatttct
     2821 cgacgttgaa gtcgatcgca ttggcgcggg tgggccgaag gaacggattg ccgccgctgc
     2881 ccttgatgat gccctcggcc ttgtcgagcc cgtagctcac cgcgatccgc atctggtcga
     2941 gccgcggccg ctgcatctcg cgggcgaggg cgaggcggaa gacccagtcg ctctcgaggc
     3001 gaagcgacag gttgaggctt ggcaggatgt cccagaactt gacccctgcc tcggccggag
     3061 tgatcacccc gccgacgatg acgttgccgt tcgacgactg atcggtccgg accgcctgca
     3121 cgccgacatt gccggtgagc gtggcgctgc cgagctcggc ccggatatcc gcctggacat
     3181 agccggtcag aagcttctcg ttgaccgtat aggccttggc gacgacgtcc ggattcgcgc
     3241 tgttcgacgc cagcgaatag acattgcccg cggccagcag ctcacgcggg tcgtagctga
     3301 ccatcgggcc gaggccgaga tagttgaggt tggtcggctc gagcaggaac tgcgtcggga
     3361 tcggcgcctc cagcgcgccg cccgcgaggc ggatcagcgc ctcgtccggg cgaaggctct
     3421 tgtcgcggag cgtatagttg aagccggcct tgatcccgga gaggaagccg ctctcgagct
     3481 cccgctcgag ctgcgcgcgg aactgccaga gctcgtcgtc gacgattcgg ttgttgtaat
     3541 agccgacctg cggcaggact ccgccgcccc agccctgcgg
//
LOCUS       DASVQZ010000024         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_102741, whole genome shotgun sequence.
ACCESSION   DASVQZ010000024 DASVQZ010000000
VERSION     DASVQZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102741"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VF547_00670"
     CDS             complement(<1..460)
                     /locus_tag="VF547_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8261366.1"
                     /translation="MAEEGDIACVVASVISSSLNCLLLLDEKGGVLEFNRAAEEAYGY
                     TAAEIVGRPIWSIIVRNASREEDEDALDAYIENGDAERGLRVVVDVRMKCGEVVPLEI
                     SVTSLAIGGQRRFLLSPRDLSARRANDELAESSRRLELAVEGAKLGTWTFD"
     gene            637..780
                     /locus_tag="VF547_00675"
     CDS             637..780
                     /locus_tag="VF547_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261367.1"
                     /translation="MTAINAKKSRLQLVLLAAAALGSSSAVLSGAVTVLAMQQPAHVS
                     TLA"
     gene            805..1716
                     /locus_tag="VF547_00680"
     CDS             805..1716
                     /locus_tag="VF547_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261368.1"
                     /translation="MYHDFNFGDVLASSVKAAWQVEDVLPAGAGLDFGRRFLPEKFAR
                     TGEIPFLGDDEKRILNQIRGHEYLTIFGLVEEFILPFVLDHARPQLNGDDHRVRALLN
                     FASEEAKHIHLFKLFHQRFTEAFGTPCEAIGPGEAIAAEVLRHDPLSVALVILQVEWM
                     TQSHYVGSVRDDHSLDPLFKSLLKHHWMEEAQHAKLDTLMVEALADGRDEAGIMAAVD
                     GYLEIGAFLDSGLAEQTRLNLDAFERATARKLGDQRREHLQAQQHQALRWTYLGSGMV
                     HPKFTATLAGLSPAARDRIAAVAPAFS"
     gene            1738..2295
                     /locus_tag="VF547_00685"
     CDS             1738..2295
                     /locus_tag="VF547_00685"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8261369.1"
                     /translation="MLDITIRPPVNGIDLAALDQTVAEVEHNPRLGQVAFRVKTDWQG
                     QTRSETTIDSFSLAGEVIPRSFTIVADEPHELLGTNSAPNPQELLMSAVNACMVVGYV
                     AQASVRGITLDSCRIETEGELDLRGFLGIDEGVPTGYRRISYTVSLEGDGTREQFEDI
                     HQAVMATSPNYFNMAQPIQMVGRLA"
     gene            2486..>3065
                     /locus_tag="VF547_00690"
     CDS             2486..>3065
                     /locus_tag="VF547_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEX8261370.1"
                     /translation="MNGSSRGGDVRTGHRRFSASIAILALLSACGGGGGGGSGGGGGG
                     GGGPPAPPPPPPPPSVAVCSLLDRQNWALAQLNEWYLFPELMAANVNPADHSSVQSYV
                     DALVAPARAQGKDRFFTVAESLSASNAFFNSGTSAGFGLRFSYDQAGRVFASEAFEGG
                     PALAAGIDRGAEILAVGTTGADLKPVSELLASG"
BASE COUNT          502 a         1104 c          954 g          505 t
ORIGIN      
        1 tgtcgaaggt ccaggtgccg agcttggcgc cttcgacggc gagctccagg cggcggctcg
       61 attcggcaag ctcgtcattg gcccttcgcg ccgagaggtc tcgcgggctc agcaggaacc
      121 ggcgctgtcc gccgatggcg agcgaggtga cgctgatctc gagcggcacc acctcgccgc
      181 atttcatccg gacgtcgacg acgaccctca agcccctctc cgcgtcgcca ttctcgatat
      241 aggcgtcgag cgcgtcctca tcctcctcgc gcgaggcgtt gcggacgatg atgctccaga
      301 tcggccggcc gacgatctcc gcggcggtat agccataggc ttcctcggcg gcccggttga
      361 actcgagcac cccgcccttc tcgtcgagca gcaggaggca gttcagcgag gagctgatca
      421 cgctcgcgac gacgcaggcg atatcgcctt cttcagccat ccccattccc cgacccccca
      481 gtcgaggccg ttataacggc gatggaagcc ggcagaaacc gcctttctgt aacaattgac
      541 acaattttcc ggcccgcgcg gaatggcccc gacaagtgga gcctccagaa gggcgtggtc
      601 gcggcgatcg atgccgcgcc caaatggaga ctgaaaatga ccgccatcaa cgccaagaag
      661 agccgcctgc agctggtgct gctcgccgcg gccgcgctgg gatcgagctc cgccgttctg
      721 agcggcgctg tgaccgtcct cgcgatgcag cagcccgccc acgtctcgac gctcgcctga
      781 gcgaagcgac cggaagggct ttggatgtac catgatttca acttcggcga cgtcctcgcc
      841 agctcggtca aggcggcctg gcaggtcgag gacgtcctgc ccgccggcgc cggcctcgat
      901 ttcggccgcc ggttccttcc cgaaaaattc gctcgtaccg gcgagatccc cttcctcggc
      961 gacgacgaga agcgaatcct caaccagatt cgcggccatg aatatctgac gatcttcggc
     1021 ctcgtggagg agttcatcct ccccttcgtg ctcgaccatg cccgcccgca gctgaacggc
     1081 gacgaccacc gggtccgtgc gctcctcaac ttcgcgagcg aggaagccaa gcacatccac
     1141 ctcttcaagc ttttccatca gcgcttcacc gaggccttcg gaacgccctg cgaggcgatc
     1201 ggcccgggcg aggccatcgc cgccgaggtg ctccgccacg acccgctgtc ggtggcgctc
     1261 gtcatcctcc aggtcgaatg gatgacccag agccattatg tcggctcggt ccgcgacgac
     1321 cactcgctcg acccgctgtt caagagcctc ctcaagcatc actggatgga ggaagctcag
     1381 cacgccaagc tcgacacgtt gatggtcgag gccctggccg acggccgcga cgaggccggg
     1441 atcatggccg cggtggacgg ctatctcgag atcggcgcct tcctcgattc gggactggcg
     1501 gagcagaccc ggctcaatct cgacgccttc gaacgcgcca ccgcccggaa gctgggggac
     1561 cagcgccgcg agcatctgca ggcgcagcag catcaggcgc ttcgctggac ctatctcggc
     1621 tcgggaatgg tccaccccaa gttcacggcc accctggccg gcctttcccc ggccgctcga
     1681 gaccgcatcg ccgcggtcgc cccggccttc agctgatcac ccgtggagaa taatatcatg
     1741 ctcgacatta cgataaggcc gccggtcaac ggcatcgacc tcgcagcgct cgaccagacc
     1801 gtcgccgagg tcgagcacaa tcccaggctc ggacaggtcg ccttccgggt gaagaccgac
     1861 tggcagggcc agacccgctc cgagacgacg atcgactcct tcagcctggc cggcgaggtc
     1921 atcccgcgca gcttcaccat cgtcgccgac gagccgcacg agctgctcgg caccaacagc
     1981 gcgcccaacc cgcaggagct gctcatgtcg gcggtcaacg cctgcatggt ggtcggctat
     2041 gtcgcccagg cctcggttcg gggaatcacg ctcgactcct gccggatcga gaccgagggc
     2101 gagctcgacc ttcgcggctt cctcggaatc gacgagggcg ttccgaccgg ataccggagg
     2161 atcagctaca ccgtctcgct cgagggcgac ggcacccgcg agcagttcga ggacatccac
     2221 caggcggtga tggccacctc gcccaactac ttcaacatgg ctcagccgat ccagatggtc
     2281 ggccggctgg cctgactcga ttcgcggccg ccttcggctg ctcaccctcc ccgtcccccg
     2341 ggaagactgg accccggcgt tccacggaac gccggggttt tttcgggcgc gccgcccgac
     2401 tccgccccgc gattcgaagg ctcgaatcga gctgtacgaa cagtccccga ttgtgcacca
     2461 tcgcggccga tggggaggtg tgcgggtgaa cggaagttcc aggggcggcg acgtccgcac
     2521 aggtcacagg cgtttctccg cctcgatcgc gatcctcgcg ctgctttcgg cctgcggtgg
     2581 aggcggaggc gggggtagcg gcgggggagg cggcggaggc ggaggccctc cggcgccccc
     2641 gcctccgccg cctcccccgt ccgtcgccgt ctgctcgctg ctcgaccgcc agaattgggc
     2701 cctcgctcag ctcaacgaat ggtatctgtt ccccgagctg atggccgcca acgtcaatcc
     2761 cgcggaccat tccagcgtcc aatcctatgt cgacgccctg gtcgccccgg cccgagccca
     2821 ggggaaggac cgcttcttca ccgtcgccga gtcgctctcg gcgagcaacg ccttcttcaa
     2881 ctcgggaacc agcgccggct tcggcctgcg attctcctac gaccaggcgg ggcgggtgtt
     2941 cgcctccgag gctttcgagg gcgggccggc gctcgcggcg ggaatcgacc gcggcgccga
     3001 gatccttgcc gtcggcacca ccggggccga cctcaagccc gtttccgagc ttctggcgtc
     3061 gggcg
//
LOCUS       DASVQZ010000025         3536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_583700, whole genome shotgun sequence.
ACCESSION   DASVQZ010000025 DASVQZ010000000
VERSION     DASVQZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583700"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VF547_00695"
     CDS             <1..326
                     /locus_tag="VF547_00695"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8261371.1"
                     /translation="MPPRLLPHVIGSPDFIFNNLFLRVQKIDARARSSMADDLKAGRP
                     TEIDHLNGEVVRLAKRLGRNAPVNEAIVSLVRQAEAGVEHLWTPARLRAHVLEGHRAA
                     PIFGY"
     gene            389..3085
                     /locus_tag="VF547_00700"
     CDS             389..3085
                     /locus_tag="VF547_00700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8261372.1"
                     /translation="MRPFATASLFALAMAGAAWTGEAAAPTPAPAPSAAAAVARQIPT
                     QLPSNVRPVMYGILVKPDPANLRFEGSVLTHIEVLRATDSIVMNAADLDIRSAELLSG
                     VDGSVAASGISLDPDKQIVTLRFAKPIAPGRYRLAIEYSGKIYTQAAGLFALDYDSPE
                     GRKRALFTQFEAPDARRFFPGWDEPQFRTPYSLTVTVPPGQDAISNMPQATSDLQADG
                     RRMISFHTTPAMSSYLLFLAVGEFDRITTKVAGTEIGVVAKRGDGEKGRWALESSARI
                     LPWYNDYFGTPYPLPKLDNVAGPGSSQFFGAMENWGAIFSFESILLVDPAITTEERRQ
                     SIFEVAAHEMAHQWFGDLVTMAWWDDLWLNEGFASWMATKATTALHPEWEPELGTVAG
                     RESAINLDSVASTHPVVQRVSTVEQISQAFDAITYRKGEAVITMLEDYVGEAAWRRGV
                     QDYIRTHRLKNTQTDDLWQAIEKAAGKPVTAIAHDFTLQPGVPLIRVEKAPCRGGRSQ
                     LFLRQGEFSRDRQGRPAKAWRVPVIAASQGKAKVRTLVSGGAARLSLPGCGPIVVNSG
                     QTGYYRTLYARPLLTRLKAGFAALKPIDQIGLLADNWALGLAGYQSAAEALDMVDAVP
                     ADANAQVWSRVATILKSVHNLYEGDPERQAMVARYASARLSPVLGRIGWAARSGEAPT
                     VPVLRADLIQTLGEMGDKAVVAEADRRLAAGDPLATAGPLRTTILAVTARNVDPAGWE
                     RLRAQARAERNPLVKAQLYRLLGTARDPALARRALDLALTDEPGATTSAGLISAVAAE
                     HPDLAYDFAIRNREKVEGLVDISSRSRYLAGLGSASADPSMIGKLEDYAKRHLTPESR
                     GRVDVAIAAIRDRVRVRTTRLQDITRWLEARYRSVPNPAERL"
     gene            complement(3063..>3536)
                     /locus_tag="VF547_00705"
     CDS             complement(3063..>3536)
                     /locus_tag="VF547_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX8261373.1"
                     /translation="LGGDLNWTMIGYGALSGAGLIMLDEMLGRAKTLRLPPLAVGIGI
                     YLPMAAVLPVVVGAVIGFFYDRWADRTGRPEFARRMGVLTATGLIVGESLWGVVFAGI
                     VAGSDSDTALVLVGEGFEPFALAGGTILFVLLSVALYRRTMVLVRRSAATGARPG"
BASE COUNT          568 a         1309 c         1159 g          500 t
ORIGIN      
        1 cgatgccgcc caggctgctg ccccacgtca tcggctcgcc cgacttcatc ttcaacaacc
       61 tgttcctccg ggtccagaag atcgacgccc gggcgcgctc gtcgatggcc gacgacctca
      121 aggccggccg gccgaccgag atcgaccacc tgaacgggga ggtggtaagg ctcgccaagc
      181 ggctcgggcg caacgccccg gtcaacgagg cgatcgtctc gctggtgcgc caggcggagg
      241 ccggagtcga gcatctgtgg acgccggcgc ggctccgggc ccatgtgctc gaaggtcacc
      301 gggcggcacc gatcttcggc tactgagttt gacacgacag gcaccttgcc gccacggcag
      361 agcgtcctgt tcccctgcgg agtccttcat gcgcccattc gccaccgcct ccctattcgc
      421 cctcgcgatg gccggcgcgg cctggacggg cgaggccgcg gcccccactc cggctcctgc
      481 cccttccgcc gccgccgccg tcgcgcgcca gatcccgacg cagcttccct cgaacgtgcg
      541 gccggtcatg tacggcatcc tcgtcaagcc cgacccggcc aatctgcggt tcgagggctc
      601 ggtcctcacc catatcgaag tgctgcgggc gaccgactcg atcgtgatga acgcggccga
      661 cctcgacatc agatcggccg agctgctcag cggcgtggac ggcagcgtgg cggcgagcgg
      721 catctcgctc gatcccgaca agcagatcgt gacgctgcgc ttcgccaagc cgatcgcgcc
      781 cgggcgctat cgcctcgcga tcgaatattc cggcaagatc tacacccagg ccgccggcct
      841 gttcgcgctc gactatgaca gccccgaagg caggaagcgg gcgctgttca cgcagttcga
      901 ggcccccgac gcccgccgct tcttcccggg gtgggacgag ccccagttcc gaacgcccta
      961 cagcctcacc gtcaccgtcc ccccgggtca ggacgcgatc agcaacatgc cgcaggccac
     1021 ctccgacctc caggcggacg gccggcggat gatcagcttc cacaccaccc ccgccatgtc
     1081 gagctatctg ctcttcctgg cggtgggcga gttcgaccgc atcaccacca aagtggccgg
     1141 caccgagatc ggcgtcgtcg ccaagcgcgg cgacggcgag aaaggccgct gggcgctgga
     1201 gagctcggcc cggatcctgc cctggtacaa cgactatttc ggaacgccct acccgctccc
     1261 gaagctcgac aacgtcgccg ggccgggcag cagccagttc ttcggggcga tggagaattg
     1321 gggcgcgatc ttctcgttcg aatcgattct cctcgtcgat cccgccatca ccaccgagga
     1381 gcgtcggcag agcatcttcg aggtcgcggc gcacgagatg gcgcaccaat ggttcggcga
     1441 tctcgtcacc atggcctggt gggacgatct gtggctcaac gaaggcttcg cttcgtggat
     1501 ggcgaccaag gcgaccaccg cgctccatcc cgaatgggag ccggagctcg gcaccgtcgc
     1561 gggacgcgag tcggcgatca acctcgattc ggtcgccagc acccatcccg tcgtgcagcg
     1621 ggtcagcacc gtcgagcaga tcagccaggc gttcgacgcg atcacctatc gcaagggcga
     1681 ggcggtgatc accatgctcg aggactatgt cggcgaggcg gcgtggcgcc gcggggtcca
     1741 ggactatatc cgcacccacc ggctcaagaa cacgcagacc gacgacctgt ggcaggcgat
     1801 cgaaaaggcg gcgggcaagc cggtgacggc catcgcgcac gacttcacgc tgcagcccgg
     1861 agtgcccctg atccgggtcg agaaggcccc ctgccgcggc ggccgctcgc agctgttctt
     1921 gcggcagggc gagttcagcc gcgaccgcca gggccgcccc gccaaagcgt ggcgggtgcc
     1981 ggtgatcgcc gcgtcccaag gcaaggcgaa ggtgcggacg ctggtgagcg gcggcgcggc
     2041 ccggctgagc cttccgggct gcggtccgat cgtcgtcaac agcggccaga ccggctatta
     2101 tcggacgctc tacgcccgac ccctgctcac ccggctcaag gcaggcttcg ccgccctgaa
     2161 gccgatcgac cagatcggcc tgctcgcgga caattgggcg ctcggccttg ccggctacca
     2221 gtccgccgcc gaggctctcg acatggtcga cgcggtcccg gccgacgcca atgcccaggt
     2281 ctggagccgg gtcgcgacca tcctcaagag cgttcacaat ctctacgagg gcgaccccga
     2341 gcgccaggcg atggtcgccc gctacgcctc ggcccggctc tcgccggtcc tcggccgaat
     2401 cggctgggct gccaggagcg gcgaggcgcc gaccgttccg gtgctccgcg ccgatctgat
     2461 ccagacgctg ggggagatgg gcgacaaggc ggtggtcgcg gaagccgatc gccgactcgc
     2521 ggcgggcgac ccgctggcca cggccggtcc gctgcgcacg accatcctcg ccgtcaccgc
     2581 ccgcaacgtc gatccggccg gctgggagcg cctcagggcc caggcccgcg ctgagcgcaa
     2641 ccctctggtc aaggcgcagc tctaccggct tctcggcacc gcccgcgacc ccgccttggc
     2701 ccggcgggcg ctcgatctcg ccctgaccga cgagccgggc gcgacgacca gcgccggcct
     2761 gatctccgcc gtggcggcgg agcatcccga tctcgcctac gatttcgcga tccgcaaccg
     2821 cgaaaaggtc gaaggtctgg tcgatatctc ctcgcgatcg cgctacctcg ccggcctcgg
     2881 cagcgcttcg gccgacccgt cgatgatcgg caagctcgag gattatgcga aacgccatct
     2941 gacgccggag tcgcgcggcc gggtcgacgt cgccatcgcc gcaattcgcg accgggtgcg
     3001 ggtgcgcacc acccggctgc aggacatcac tcggtggctg gaggcccgct accgctcggt
     3061 gcctaacccg gccgagcgcc tgtagcggcg gaacgccgga ccagcaccat cgtccgccga
     3121 tagagcgcca ccgacagcag cacgaacagg atcgtcccgc cggccagcgc gaaaggctcg
     3181 aagccctcgc cgaccaggac cagggccgtg tcgctgtccg agccggcgac gatgccggcg
     3241 aagacgactc cccacaggct ctcgccgacg atcaggccgg tggcggtgag aacccccatc
     3301 cttcgggcga attcggggcg ccctgtacgg tcggcccagc ggtcgtagaa gaaaccgatc
     3361 acagcgccga ccaccaccgg cagcaccgcg gccatcggca ggtagatgcc gatgccgacc
     3421 gcgagcggcg gcaggcgcag cgtcttggcg cggccgagca tctcgtcgag catgatgagc
     3481 cccgcgccgc tcagcgcccc gtagccgatc atcgtccagt tgaggtcgcc gccgag
//
LOCUS       DASVQZ010000026         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_6564,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000026 DASVQZ010000000
VERSION     DASVQZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6564"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VF547_00710"
     CDS             complement(<1..1453)
                     /locus_tag="VF547_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8261374.1"
                     /translation="MTYTPPVADQRFVLEHVVGMAELAGHDAYAEATPDLVDAILEGA
                     GAFAAGEFAPLNRVGDEVGAKWTAEGVTMPPGFRQAYSAYVEGGWGTLAGPPEHGGQG
                     LPLVLATVVMEDLGAANMAFSLVTMLSAGAVEALRHHGSPDMQARWLPKLVTGEWTGT
                     MNLTEPQAGSDVGALKTRAEPAGDGSWRVSGQKIFITFGDHDLADNIVHLVLARTPGA
                     PPGTKGISLFLVPKVLPDGSANDLRCVSIEHKLGIHASPTCVMAFGDQGDCRGWLIGE
                     EMGGMRAMFTMMNNARLNVGLQGVSIGERAAQAAVAYATQRVQGMRGGRPAAIVEHPD
                     VRRMLLRMTALTQAARALCYYAAGQTDRGTLGDKAAQARADLLTPLVKAYGTDVGCEV
                     ASLGVQVHGGMGFVEETGAAQHYRDIRIAPIYEGTNGIQAADLVGRKLGLEGGETLRR
                     LLADVRSEAGEGAVGALADVVEVAAERVSAMAAD"
     gene            complement(1619..>1743)
                     /locus_tag="VF547_00715"
     CDS             complement(1619..>1743)
                     /locus_tag="VF547_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX8261375.1"
                     /translation="LRKAGERTTEAVWRRIGSLLDTFTPSECANYIRNSGYASC"
BASE COUNT          255 a          660 c          555 g          273 t
ORIGIN      
        1 cgtccgccgc catcgccgac acacgctccg cggcaacttc cacaacatcc gccagcgccc
       61 caaccgctcc ctcccccgct tccgacctca cgtcggcgag cagccgccgc agcgtctcgc
      121 ccccttccag cccgagcttc cggccgacca ggtcggccgc ctggatcccg ttcgtcccct
      181 cgtagatcgg ggcgatccgg atgtcccggt aatgctgcgc cgcccccgtc tcctcgacga
      241 agcccatgcc gccatggacc tgcacgccga ggctcgccac ctcgcagccg acgtcggtgc
      301 cataggcctt gaccagcggc gtcagcaggt ccgcccgcgc ctgagccgcc ttgtcgccga
      361 gggttccgcg gtcggtctgg ccggcggcat agtagcacag cgcccgcgcc gcctgggtca
      421 gcgccgtcat cctcagaagc atccgccgga cgtcgggatg ctcgacgatc gccgccggcc
      481 gccccccgcg catcccctgc acccgctgcg tcgcataggc gaccgcggcc tgggccgccc
      541 gctcgccgat cgacacgccc tgcaggccga cgttgaggcg cgcattgttc atcatcgtga
      601 acatcgcccg catccccccc atctcctcgc cgatcagcca gccgcggcaa tcgccctggt
      661 cgccgaacgc catcacgcag gtcggcgacg cgtggatccc gagcttgtgc tcgatcgaca
      721 cgcacctgag gtcgttggcg cttccgtcgg gaaggacctt gggcaccagg aacaatgaga
      781 tgcccttcgt ccccggcggc gccccgggcg tgcgggccag caccaggtgg acgatattgt
      841 cggcgaggtc gtggtcgccg aaggtgatga agatcttctg ccccgagacc cgccagctgc
      901 cgtcgcccgc cggctccgct cgggtcttga gcgcgccgac gtccgagccc gcctgcggct
      961 cggtgaggtt catcgtcccc gtccattcgc cggtgaccag cttcggcagc cagcgcgcct
     1021 gcatgtcggg cgagccgtgg tgcctgagcg cctcgaccgc tccggcgctc agcatcgtca
     1081 ccagcgagaa ggccatgttg gccgcgccca gatcctccat aaccaccgtg gcgaggacca
     1141 gcggcagccc ctggccgcca tgctccggag gcccggccag agtgccccag ccgccctcga
     1201 cataggccga ataggcttgg cggaagcccg gcggcatcgt cacgccctcc gccgtccatt
     1261 tcgccccgac ctcgtcgccg acccggttca gcggcgcgaa ctcgcccgcc gcgaaggcgc
     1321 ccgcgccctc gaggatcgcg tcgaccaggt cgggggtcgc ttcggcatag gcgtcgtggc
     1381 cggcgagctc ggccatgccg acgacatgct caagcacgaa gcgctggtcg gcgacgggcg
     1441 gcgtataggt catgccggac tagtatcgag atcggcttcg ccggggaacc caatcatcac
     1501 gtctcgggac gaggctccag gccttattca cgaggctgag gcggtatccg atccgctcct
     1561 catgagtgca ggcacgggcc gaagcgacgc aaccgacgca gactagagca tgatcgtctt
     1621 agcatgaggc gtatccgctg tttctgatgt agttggcgca ttcgctgggt gtgaaggtgt
     1681 cgaggaggga tccgattcgg cgccagacgg cttcggtggt gcgttcgccg gccttgcgga
     1741 gca
//
LOCUS       DASVQZ010000027         9191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_200126, whole genome shotgun sequence.
ACCESSION   DASVQZ010000027 DASVQZ010000000
VERSION     DASVQZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200126"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..686)
                     /locus_tag="VF547_00720"
     CDS             complement(57..686)
                     /locus_tag="VF547_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8261376.1"
                     /translation="MATAIGNADKEPRTERGRKTLRRLIEAAAAEFGERGFHEAAITG
                     ITRRAGVALGTFYTYFESKEEVFRALVLDMSRATRAHVAEAVKGAPDRLAAERIGLEA
                     FIAFTRRHPELYRIIEEAQFVAHDVYREHYLSFADAYRRNLAGARERGEIAAGARGER
                     EDEARAWALIGMSVFLGMRYGLWDEDLSPAEVADIAIELVSEGLRKRRR"
     gene            complement(803..1603)
                     /locus_tag="VF547_00725"
     CDS             complement(803..1603)
                     /locus_tag="VF547_00725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8261377.1"
                     /translation="MVASDTAFAGSIPKFYHRCLGPFLFEPYAEDLARRAAGLGPRRI
                     LETAAGTGIVTAALARALPEAEIIATDLNPDMLRVARSHVDSPRVAFATADAQSLGFA
                     EARFDLVVCQFGAMFFPDRVGAYREARRVLDRGGTLLFNVWDRLDANPASQAVSEALA
                     ALFPNDPPGFLDRVPFGYHDPARIEADLRAAGFESIAVETVAKRSRGSAREVAPGLCQ
                     GSPLRAEIVARAPDRLDEATEAAVAALVRRCGDPVDAAMSAHVFTARA"
     gene            complement(1624..2103)
                     /locus_tag="VF547_00730"
     CDS             complement(1624..2103)
                     /locus_tag="VF547_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEX8261378.1"
                     /translation="MDQTLPVSTIAHLIQLAIAPVFLLAGIAGILNVLAQRLSRVVDR
                     GRALERDFASFDEATRARASEELLIIIRRMRIVNLAITACTASALFVCLVVAILFVAD
                     LAEIPFARPIAVLFIVAMTLLIVGLTLFLAEIRLAARFVSIRHDLLPLAPPRRSTDD"
     gene            complement(2121..4034)
                     /locus_tag="VF547_00735"
     CDS             complement(2121..4034)
                     /locus_tag="VF547_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8261379.1"
                     /translation="MAWRLTAAGWLGCALLQLVLYLRPSPYGGPFLVAWKAFILRPLA
                     YELLAVWLLALPFLLLWLVLYRRPLKSPRWRLAHWALAAVMAVNLAVTAFDHELYRFL
                     GLRLGPNFLDVYADPATLGDSLFLNVLRGDRGGPFLSPVLCLGAPALYSWWAARRIRR
                     PAAALRLRTALTLAILPLAAGAAAYSLAHAKFRLSRLEPALFAAIRDFAWSYEDDAPP
                     GDVAALAAAWQAEWLAGGTDRGWRFTRPFYPYLREPDRPSVPRPKDRWNVLIVQLESV
                     RGVDTGHLRPDRRPSPTPFLDSLAARPDAAVWTRALTFGPPSINGRFALQCSLTPSSR
                     RFITAQTRATFHCLPDALRRLGYRAEMFAAGDIDMDNSTLWIQRMYDRLWRYPEEGQR
                     DRQIFRHAAARIREIGRRGPFIATLISASNHHPFRSPEPELDIAGHGHVRQRILNTER
                     YTDDVVRELIESLRAEPWFERTLIVVNSDHGYNLGEHGGLTGAYSLYRESVWTPFLIV
                     GPHPRLPAGRHDAIVTLLDVAPTIADLIGLRAANPWHGHSLLTLGPGRPVRFGFRAGV
                     MAEAGGWSAVSDPTDGRPRLYDSRFDWLQRRDLAGLHPALARRMLDDADRGRRFNDYL
                     LRHGRLWPPDPSR"
     gene            4162..4617
                     /gene="nusB"
                     /locus_tag="VF547_00740"
     CDS             4162..4617
                     /gene="nusB"
                     /locus_tag="VF547_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX8261380.1"
                     /translation="MPSSPSRSRSRSAARLAAVQALYQREMEETPVPKLLHEFHHHRL
                     GATIEGVEYEEAEVDFFDDLVSGVAAREAELDAAISARLTADWPLHRLDRPMRQILRA
                     GAYELAARIDVPTGSVISEYVDVAKAFYGQKEAGFVNGILDSVAKDVRK"
     gene            4716..5636
                     /gene="thiL"
                     /locus_tag="VF547_00745"
     CDS             4716..5636
                     /gene="thiL"
                     /locus_tag="VF547_00745"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX8261381.1"
                     /translation="MSSESTFIESLRALATHPAARGLADDAAVLEFAGRALVLTHDML
                     VEGVHYRPDDPPADVAWKLVAVNLSDLAAKGARPIGVLLGYSLSEDEWDEAFAAGLGT
                     ALGAFGIPLLGGDTVRGGGARTLALTALGEADGPVPSRAGGRPGDHLWVSGTIGDSGA
                     GLRGLGEALVERYRNPRPRLEAGRSLVPLVAAMMDVSDGLLIDASRLAAASGVAARID
                     LTLVPLSEALLAASGDSRETRLEAATAGDDYELLFAARPDRAGEILALQDGLGLPFSR
                     IGTLAAGTGLRLTAGDDPVPLPPRLGWEHR"
     gene            6015..8186
                     /locus_tag="VF547_00750"
     CDS             6015..8186
                     /locus_tag="VF547_00750"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX8261382.1"
                     /translation="MTVVLIAIACGVAAVLYGILTSMQVLKASAGNERMVAVAAAIQE
                     GAKAYLFRQYSTIGVVGIVVAILVFLFLGPIQAAGFVLGAVLSGAAGFIGMNISVRSN
                     VRTAEAARTSLQRGLTVAFRAGAITGMLVAGLALLAISVFFYILVDAMNFAPDDREVV
                     DALVALAFGASLISIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYVVTVGATMVLIALLVNAGAEQMLALMSLPLAIGGVCILTSI
                     IGTYFVRLGRSQSIMGALYKGFWLTAGLSIPAIFYVMWQTLGGDMNSVIGGATEAAGA
                     DVSTDVAATAVGGGFTGMDLFWCSLVGLAVTGLIVWITEYYTGTNYRPVRSIAKASQT
                     GHGTNVIQGLAISLESTALPTIVICAGIIVSYQLAGLIGIAFAATAMLALAGMVVALD
                     AYGPVTDNAGGIAEMAGMEEDVRTRTDALDAVGNTTKAVTKGYAIGSAALGALVLFGA
                     YTTDLEHYAGELGIGAAGIDFSLSNPYVVVGLLLGALLPYLFGAMGMTAVGRAAGNVV
                     LDVREQFRSNPGIMDGTSRPNYARTVDLVTKAAIKEMIIPSLLPVLTPIVVYFVIAWI
                     AGREQGFAALGALLLGVIVSGLFVAISMTSGGGAWDNAKKYIEDGNYGGKGSEAHKAA
                     VTGDTVGDPYKDTAGPAVNPMIKITNIVALLLLAALAGGAG"
     gene            8305..>9191
                     /locus_tag="VF547_00755"
     CDS             8305..>9191
                     /locus_tag="VF547_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261383.1"
                     /translation="MLRPIVLAAAVLLASSAAPAAPPPLIPVEAFGALPSFANPKISP
                     DGQRVLATADVDGRKAVIVYRLDAADGRFTRINLGELEVTGARWAGNGRILLTVFGKT
                     LYQGVEVPMSRHFAYDFDTGKMRALGGGRIGGLWGGELIHVDPDGAFALLAAQSTVWT
                     APSVLRVDLANGDTKEIVAPEGGVWDWYADSTGTVRAGLGVRGDRWFLLYRDKEGGRF
                     RRTEAKREVAQSATDLDYLLPVVGSDKGYAVANKATGRYGIYRYDFATDSLGEAVFEH
                     PEVDVEGVAYSPRTGELDS"
BASE COUNT         1348 a         3178 c         3319 g         1346 t
ORIGIN      
        1 gaccgaatcc ggagtgagct cggcgcggcg ggcgatcagg tcgggcagat cggcgatcac
       61 cggcggcgct tcctcagccc ctcggatacc agctcgatgg cgatgtcggc gacctcggcc
      121 ggcgacaggt cctcgtccca caggccgtag cgcatgccga ggaagacgct cattccgatc
      181 agcgcccagg ccctcgcctc gtcctcgcgc tcgccgcgcg cgccggcggc gatctcgccg
      241 cgctcgcggg cgccggcgag gttgcggcga taggcgtcgg cgaacgagag gtaatgctcc
      301 cggtagacgt cgtgggcgac gaactgggcc tcctcgatga tccggtagag ctcgggatgg
      361 cggcgggtga aggcgatgaa cgcctcgagg ccgatccgct cggcggcgag ccggtcgggc
      421 gcgcctttca ccgcctcggc gacgtgggcg cgcgtggcgc ggctcatgtc gaggacgagc
      481 gccctgaaca cctcctcctt gctctcgaaa taggtgtaga aggtgccgag agccactccg
      541 gcgcggcggg tgatgccggt gatcgccgcc tcgtggaagc cgcgctcgcc gaactccgcg
      601 gccgccgcct cgatcagccg ccgaagcgtc ttgcggccgc gctcggtccg cggctccttg
      661 tcggcattgc cgatcgctgt cgccatgcgc cctccctcgc cggaaagttg aacccgtttt
      721 cagcttggcg caagctcggc caggatccgc cggacctgcc tcggctgggc gggcttatcc
      781 ttctgacccg ggcgccggag attcacgccc gcgcggtgaa gacgtgggcg gacatggcgg
      841 cgtcgaccgg gtcgccgcag cggcggacca gggcggcgac ggccgcctcg gtggcctcgt
      901 cgagccggtc gggggcgcgc gccacgatct cggcgcgcag cggcgagccc tggcagaggc
      961 cgggggccac ctcgcgggcc gagccgcggc ttcgcttcgc cacggtctcc acggcgatcg
     1021 actcgaatcc ggcggcgcgc aggtcggcct cgatcctggc cgggtcgtga tagccgaacg
     1081 ggactcggtc gaggaagccg ggcggatcgt tggggaacag ggcggcgagc gcttcggaca
     1141 ccgcctgcga ggcgggattg gcgtcgagcc ggtcccagac gttgaacagc agggtcccgc
     1201 cgcggtcgag gacccggcgc gcctcccgat aggcgccgac ccgatcggga aagaacatcg
     1261 cgccgaactg gcagacgacg aggtcgaagc gggcctcggc gaagccgagc gactgggcat
     1321 cggcggtggc gaacgcgacc cgcggcgaat cgacgtggga cctcgccacc cgaagcatgt
     1381 ccgggttgag gtcggtggcg attatctccg cttcgggcag ggcgcgcgcc agcgcggcgg
     1441 tgacgatgcc ggtgccggcg gcggtctcca atatccgtcg cgggccgagg ccagccgcgc
     1501 gcctcgccag atcctccgca taaggctcga acaggaacgg cccgaggcag cgatggtaga
     1561 atttcgggat cgagcccgcg aaggcggtat cggaggcgac catggcggac gtctaggccg
     1621 gccctaatcg tctgtcgagc ggcgcggcgg cgcgagcggc agcaggtcgt ggcggatgct
     1681 gacgaaacgg gcggccagcc ggatctccgc gagaaacagg gtgaggccga cgatgagcag
     1741 cgtcatggcg acgatgaaca gcaccgcgat gggccgcgcg aacggtattt ccgcgaggtc
     1801 cgcgacgaac aggatcgcca cgacgaggca gacgaacagc gcgcttgccg tgcaggcggt
     1861 gatggcgagg ttcacgatcc tcattcgccg aatgatgatc agcagctcct cgctggcccg
     1921 cgcccgggtc gcctcgtcga acgaggcgaa gtcgcgctcg agggcgcggc cgcggtcgac
     1981 caccctcgag agccgctgcg ccagcacgtt caggatgccg gcgatccccg ccagcaggaa
     2041 gacgggggcg atcgccagct ggatcagatg ggcgatggtc gagacgggca gggtctggtc
     2101 catgtcggcg caactccggg tcagcgcgaa gggtccggcg gccacaggcg gccgtggcgg
     2161 agcagatagt cgttgaaccg gcggccgcgg tcggcgtcgt cgagcatccg ccgggcgagc
     2221 gccggatgga gtccggccag gtcccggcgc tggagccagt cgaatcgcga gtcgtagagc
     2281 cgcggccgtc cgtcggtcgg gtcgctgacg gcgctccagc cgcccgcctc cgccatcacg
     2341 ccggcgcgga agccgaagcg gaccgggcgg ccgggcccga gcgtgagcag gctgtggccg
     2401 tgccagggat tggccgcgcg caggccgatc aggtcggcga tcgtcggggc gacgtcgagc
     2461 agggtgacga tcgcgtcgtg gcggccggcg ggcaggcgcg gatgcgggcc gacgatcagg
     2521 aacggggtcc agaccgactc gcgatagagg ctgtaggcgc cggtgaggcc gccatgctcg
     2581 cccaggttgt agccatggtc gctgttgacg acgattaggg tgcgctcgaa ccagggctcc
     2641 gcccgaagcg attcgatcag ctcgcgcacg acgtcgtcgg tgtagcgctc ggtgttgagg
     2701 atgcgctggc ggacatggcc gtggccggcg atgtcgagct cgggctcggg cgagcggaac
     2761 gggtggtggt tgctcgccga gatcaaggtg gcgatgaacg gcccgcgccg tccgatctcc
     2821 cggatccggg cggcggcgtg gcggaagatc tggcggtcgc gctggccctc ctcgggatag
     2881 cgccacagcc tgtcgtacat gcgctggatc cacagggtgc tgttgtccat gtcgatgtcg
     2941 ccggccgcga acatctcggc gcggtagccg agccggcgca gcgcgtcggg caggcagtgg
     3001 aaggtcgccc tcgtctgggc ggtgatgaac cggcgcgacg agggcgtcag cgagcattgc
     3061 agcgcgaagc ggccgttgat gctcggcggg ccgaaggtca gggcccgggt ccatacggcc
     3121 gcgtccgggc gggcggcgag gctgtcgagg aagggcgtgg gcgaggggcg gcggtcgggc
     3181 cgaaggtggc ccgtgtcgac gccgcggacc gattcgagct ggacgatgag gacgttccag
     3241 cggtccttgg gcctcggcac ggaaggacgg tcgggctccc gcagataggg atagaagggc
     3301 cgggtgaagc gccagccgcg gtccgtgccg ccggccagcc attccgcctg ccaggccgcg
     3361 gccagcgccg ccacgtcgcc gggcggagcg tcgtcctcgt agctccaggc gaaatcgcgg
     3421 atcgcggcga acagggccgg ctccagccgg gacagccgga acttggcgtg cgcgagcgaa
     3481 taggcggcgg cgccggcggc gagcggcagg atcgcgaggg tcagcgccgt ccgcagccgc
     3541 agcgcggccg ccgggcgccg gatcctgcgg gcggcccacc acgaatagag cgccggggcg
     3601 cccaggcaga ggaccggcga caggaacggc ccgcccctgt cgccgcgaag gacgttgagg
     3661 aacaggctgt cgccgagcgt ggcgggatcg gcatagacgt cgaggaaatt gggcccgagc
     3721 ctcagcccga ggaagcggta gagctcgtgg tcgaaggcgg tgacggcgag gttgacggcc
     3781 atcaccgcgg ccagcgccca gtgcgccagc cgccagcgcg gcgacttcag gggccggcgg
     3841 tagagcacca gccagagcag caggaaaggc agagcgagaa gccagaccgc gagcagctcg
     3901 taggcgagcg ggcgcaggat gaacgccttc cacgcgacca ggaagggccc gccataaggc
     3961 gagggccgga ggtagaggac gagctggagc agggcgcagc cgagccagcc ggccgctgtc
     4021 agccgccacg ccatggcaaa gtggccggct ccgcgcgccg gccccgccgt ctcccgcaag
     4081 ctgcgccctc cgatctgtgc cgctcatagc atggaggatg accttggcca gcgaccccgg
     4141 cgcggctatg gccggcgtcc catgccgtcc tccccgtccc gttcgcgctc ccggtccgcc
     4201 gcccgcctcg ccgccgtcca ggcgctctac cagcgcgaga tggaggagac gccggtgccc
     4261 aagctgctcc acgaattcca ccatcaccgg ctcggagcga ccatcgaagg cgtggaatat
     4321 gaggaggccg aggtcgactt cttcgacgat ctggtgagcg gggtcgccgc tcgcgaggcg
     4381 gagctcgacg ccgccatctc ggcgcggctg acggccgact ggccgctcca ccgcctcgac
     4441 cggccgatgc ggcagatatt gcgcgccggc gcctacgagc tcgccgcccg catcgacgtc
     4501 ccgaccggaa gcgtcatctc ggaatatgtc gacgtcgcca aggccttcta cggccagaag
     4561 gaagcgggct tcgtcaacgg catcctggac tcggtggcga aggacgttcg gaagtgacgg
     4621 cgcagccgtc attgcgagcg tagcgcggca atccagtgcg gcttcggagt cagcactgga
     4681 ttgcttcgtc gcttcgctcc tcgcaatgac ggcttgtgtc gtccgaatcc accttcatcg
     4741 agtcgctgcg agcgctggcg acccatcccg ccgcccgcgg gctcgccgac gacgccgccg
     4801 tgctcgaatt cgccggccgg gccctcgtcc tcacccacga catgctcgtc gagggcgtcc
     4861 attaccgccc cgatgatccg cccgccgacg tcgcctggaa gctcgtcgcc gtgaacctct
     4921 ccgacctcgc cgccaagggc gcccggccga tcggcgtgct gctcggctac agtctaagcg
     4981 aggatgaatg ggacgaggcc ttcgccgccg gcctcggcac ggcgctcggg gctttcggga
     5041 tcccgctgct cggcggcgac accgtgcgcg gcggcggcgc tcggacgctg gcgctgacgg
     5101 cgctcggcga ggcggacgga ccggtgccgt cgcgcgccgg cgggcggccg ggcgatcatc
     5161 tctgggtcag cggcacgatc ggcgattccg gcgccgggct ccgcggcctc ggcgaggctc
     5221 tggtcgagcg ctaccgcaac ccgcggccgc ggctcgaggc cggccgcagc ctggtgccgc
     5281 tggtggcggc aatgatggac gtctccgacg gccttctgat cgacgcctcg cgcctggcgg
     5341 cggcgagcgg ggtcgcggcc cggatcgacc tgaccctcgt tccgctgtcg gaagccctgc
     5401 tggccgcatc gggcgacagc cgggagaccc ggctggaggc cgccacggcc ggcgacgatt
     5461 acgaattgtt gttcgctgcc cggccggacc gggccggcga gatccttgcc ctgcaagacg
     5521 ggctcggcct ccccttcagt cgcatcggca ctctcgccgc ggggaccggc ctgcgcctca
     5581 ccgccggcga cgacccggtg ccgctgccgc cgaggctcgg ctgggagcat cgctgaaaat
     5641 agggctttga acggcccctt gttcgtctta tgttcaaatc tccggcgagg gcgggttcga
     5701 cagccgaggc gccgtcgccg ggaatccgac gctcgtctga aaacaaagga ttctacggcc
     5761 cgacatgcgg gtcctttgtg tcttttgcgg atcgcgggcg gcgcttggcc ggtccgtgag
     5821 ggcaggcgat ttccgtgccg gactcgttca gcccgtccac ttcggtccgc tttgcggcgg
     5881 ctcggcgagc cgagcatcgg cggacggttg aatcccgcgc gcaagccccc taaagggcgc
     5941 ggcacatccg ggcgcgtccg cgccggggcc attgccctgc ccggctttcg aattccaggg
     6001 gaaggaagaa tttcatgacc gtcgtgctca ttgccatcgc ctgcggagtg gccgcggtgc
     6061 tgtacggcat cctcaccagc atgcaggtgc tcaaggcgtc ggccggcaac gagcgcatgg
     6121 tcgccgtcgc cgccgccatc caggagggcg ccaaggccta tctgttccgg caatacagca
     6181 cgatcggcgt ggtgggcatc gtcgtcgcga tcctcgtctt cctgttcctc gggccgatcc
     6241 aggccgccgg cttcgtgctc ggcgcggtgc tgtcgggcgc cgccggcttc atcggcatga
     6301 acatctcggt ccgctccaac gtgcgcaccg ccgaggccgc ccgcaccagc ctccagcgcg
     6361 gcctcaccgt cgccttccgg gcgggcgcga tcaccggcat gctggtcgcg ggcctcgccc
     6421 tgctcgccat ctcggtcttc ttctacatcc tggtcgacgc gatgaacttc gcgcccgacg
     6481 accgcgaggt cgtcgacgcc ctcgtcgcgc tcgccttcgg cgcctcgctg atctcgatct
     6541 tcgcccgcct gggcggcggc atcttcacca aggccgccga cgtcggcgcc gacctcgtcg
     6601 gcaaggtcga ggccggaatc cccgaggacg acccccgcaa cccggccgtg atcgccgaca
     6661 acgtcggcga caatgtcggc gactgcgcgg gcatggccgc ggatctgttc gagacctacg
     6721 tcgtcaccgt cggcgccacc atggtgctga tcgccctcct cgtgaacgcc ggcgccgagc
     6781 agatgctggc gctgatgagc cttccgctgg cgatcggcgg ggtctgcatc ctgacctcga
     6841 tcatcggcac ctatttcgtc cgcctcggcc gctcgcagtc gatcatgggc gcgctctaca
     6901 agggcttctg gctgaccgcc ggcctgtcca tcccggcgat cttctacgtc atgtggcaga
     6961 ccctcggcgg cgacatgaac tcggtcatcg gcggcgccac cgaggcggcc ggcgccgacg
     7021 tctcgaccga cgtcgcggcg accgccgtcg gcggcggctt caccggcatg gacctgttct
     7081 ggtgctcgct ggtcggcctc gccgtcaccg gcctgatcgt ttggatcacc gaatattaca
     7141 ccggaaccaa ctaccggccg gtccgctcga tcgccaaggc ctcgcagacc ggccacggca
     7201 ccaacgtcat ccagggcctg gcgatcagcc tcgaatcgac cgctctgccg acgatcgtga
     7261 tctgcgccgg gatcattgtc tcctaccagc tcgccgggct gatcgggatc gccttcgccg
     7321 cgaccgcgat gctcgcgctc gccggaatgg tcgtcgccct cgacgcctac ggcccggtca
     7381 ccgacaatgc cggcggaatc gccgagatgg ccgggatgga ggaggacgtt cgcacccgca
     7441 ccgacgccct cgacgcggtc ggcaacacga ccaaggcggt gaccaagggc tatgcgatcg
     7501 gctccgcggc gctcggcgcg ctggtgctgt tcggcgccta cacgaccgac ctcgagcatt
     7561 atgccggcga gctcggcatc ggcgcggccg ggatcgattt ctcgctgtcc aacccctatg
     7621 tggtcgtcgg cctgctgctc ggcgcgctcc tcccctacct gttcggggcg atgggcatga
     7681 ccgccgtcgg gcgagcggcg ggcaacgtcg tcctcgacgt gcgcgagcag ttccgctcca
     7741 atcccggcat catggacggc acctcgcggc ccaattacgc ccgcaccgtc gacctcgtca
     7801 ccaaggcggc gatcaaggag atgatcatcc cctcgctgct gccggtgctg accccgatcg
     7861 tcgtctattt cgtcatcgcc tggatcgccg ggcgcgagca gggcttcgcc gccctgggcg
     7921 cgctgctgct gggcgtgatc gtctccggcc tgttcgtcgc catctcgatg acctcgggcg
     7981 gcggcgcctg ggacaatgcc aagaaatata tcgaggacgg caattacggc ggcaagggct
     8041 ccgaggccca caaggccgcg gtgaccggcg acacggtcgg cgatccctac aaggacacgg
     8101 ccggcccggc ggtcaacccg atgatcaaga tcaccaacat cgtcgccctg ctgctgctcg
     8161 cggcgctggc cggcggcgcc ggctgatcgc gcccgaggcc cgaaagcgac aagccccgcc
     8221 cggcccagcc gggcggggct ttccttgagt cggcagcggc ttgccagacg agccggggcg
     8281 gctaggatcg ggccgggggt gaacatgctg agaccgatcg tcctggccgc ggccgtcctg
     8341 ctcgcatcga gcgcggcgcc ggccgctccg ccgccgctca ttccggtcga ggccttcggc
     8401 gccctgccct cattcgccaa tccgaagatc tcgcccgacg gacagcgtgt gctggccacc
     8461 gccgacgtcg acggccgcaa ggcggtcatc gtctaccggc tcgatgccgc cgacggccgt
     8521 ttcaccagga tcaatctcgg cgagctcgag gtgacgggcg cgcgctgggc cggcaacggc
     8581 cggatcctcc tcaccgtctt cggcaagacg ctctatcagg gcgtggaagt gccgatgagc
     8641 cggcatttcg cctacgactt cgataccggc aagatgcgcg cgctgggcgg cggccggatc
     8701 ggcggcctgt ggggcggcga gctgatccac gtcgaccccg atggcgcctt cgctctgctg
     8761 gcggcgcaat cgacggtctg gaccgccccg tccgtgctgc gggtagacct ggcgaacggc
     8821 gacacgaagg agatcgtggc tccggaaggc ggggtctggg actggtatgc ggattcgacc
     8881 ggaacggtcc gcgcggggct cggagtcaga ggcgaccgct ggttcctgct ctacagggac
     8941 aaggaaggcg ggcgattccg ccgcaccgag gcgaagcggg aggtcgcgca atcggcgacc
     9001 gacctcgact atctgcttcc ggtggtcggc agcgacaagg gctatgcagt cgccaacaag
     9061 gccaccggcc gttacggcat ctaccgctac gatttcgcca ccgactcgct gggcgaggcg
     9121 gtgttcgagc atccggaggt cgacgtcgag ggcgtcgctt actcgccgcg cacgggcgag
     9181 ctcgactcga t
//
LOCUS       DASVQZ010000028         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_103873, whole genome shotgun sequence.
ACCESSION   DASVQZ010000028 DASVQZ010000000
VERSION     DASVQZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103873"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VF547_00760"
     CDS             <1..661
                     /locus_tag="VF547_00760"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8261384.1"
                     /translation="VTDSFELGWQRRKAGTFRSLTGFYRRSRGGVTDIISDLGGGVFL
                     TTRANLATAERAGIEALANGKLSKTLTYNASATLLWNAIDSRENGIAARRSGTTGTAR
                     VNLSWQPTKKDFFQLNGSYSGRQLLPQGHRRSGPIFNLGYRRKVNDRLSLLVTGQDVL
                     SSAKQVVVFDTPTLRDRFEQRGIGRVILLGLSYNLGDQNPKRRPEPQFDFQQGGGEPV
                     Q"
     gene            756..2918
                     /locus_tag="VF547_00765"
     CDS             756..2918
                     /locus_tag="VF547_00765"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8261385.1"
                     /translation="MVQRFAFLAASAATLALGISGSALSQTAPAAAPSAAPAATAEEE
                     DPPQMQFPGWGANPADLDPAVKPGDDFDAYVNGKWKAATRIPAKYPSYGVTTNLRIGS
                     ERAVKAIIDDLAARQNPPGSLEARVADMYRAYLDTEAINRAGLAPVKPYLDRIAAVRS
                     HDELASLWSVTGYPAPMGTFVSIDRGDPNRNILYYGIGGIGLPDRDNYLVDNPRNLEM
                     RGKYLDYMTFLLGKAGHAAPRAAAESVYALEKKIAAAMWDRALSRNPELTTNRMSHAE
                     LVELARPFPIERYLAGRQILASDRFNVINVPPSADKIRSQNLTPEQLAKLGTGLPAMM
                     NLIMATPLDTWKAWMSVRLLSGAADLLPSDIDDAQFAFYGQYLQGRERQRDRWQRAVT
                     EVEGSLGEAVGKVYVERHFPPSSKASMEELVANLRLAMTENLKDLAWMTPATRTAAKA
                     KLDALRVKIGYPAEFETYDGLAVTAGDPIANRLSAARWAWADQLDELRKPVDREKWLM
                     TPQTVNAYYMPTANDMAYPAGYLQPPNFNASADPAVNYGAIGATIGHEIGHGFDDNGS
                     RYDGTGALRNWWTPEDAATFRKLGERLEAQYNAICPLDEGKKCLNGKLTLGENLGDLG
                     GLSMAYKAYQISLKGKKAPVIDGLTGDQRFFISYAQHYRTIYREPFLRQLMETDPHSP
                     SFARVNAVLRNFDPFYAAFKVKPGDKMYLPPAERVRVW"
BASE COUNT          526 a         1102 c          928 g          417 t
ORIGIN      
        1 ggtcaccgat tcgttcgagc tcggctggca gcgccgcaag gccggcacct tccggtcgct
       61 gaccggcttc taccgccggt cgcggggcgg ggtcaccgac atcatctcgg acctgggcgg
      121 cggagtcttc ctcaccaccc gggccaacct cgccaccgcc gagcgcgccg gcatcgaggc
      181 gctcgccaac ggcaagctct ccaagaccct cacctacaac gcctcggcga ccctgctgtg
      241 gaacgcgatc gactcgcgcg agaacgggat cgcggcgcgc cgctccggga cgaccgggac
      301 cgcccgggtc aatctcagct ggcagccgac gaagaaggac ttcttccagc tcaacggctc
      361 ctattccggc cgccagctcc tcccccaggg ccaccgccgc tccggtccga tcttcaacct
      421 cggctaccgc cggaaggtca acgaccgcct cagcctcctg gtcaccgggc aggacgtgct
      481 gagcagcgcc aagcaggtcg tcgtcttcga caccccgacc cttcgcgacc gcttcgagca
      541 gcgcggcatc ggccgggtga tcctcctcgg cctcagctac aatctcggcg accagaaccc
      601 gaagcgaagg cccgagcccc agttcgactt ccagcagggg ggcggggagc cggtgcaata
      661 gggcgggccc ggggcctttt gcgcaatcgc aggtaagccg tccggcgccg ccctaaacta
      721 caagcgtttt caaatcacgc tcgcggggga ggatcatggt acaaaggttc gcgtttctgg
      781 cggcgtcggc ggccacgctg gcgctcggca tctccggctc cgcactttcg cagaccgcgc
      841 cggccgccgc gccctcagcc gcgcccgccg cgacagcgga ggaagaggac ccgccgcaga
      901 tgcaattccc cggctggggc gcgaaccccg ccgacctcga ccccgccgtc aagcccggcg
      961 acgatttcga cgcctatgtg aacggcaagt ggaaggcggc gaccaggatc ccggccaaat
     1021 atccgtccta cggggtcacc accaacctca ggatcggctc cgagcgcgcg gtgaaggcga
     1081 tcatcgacga cctcgccgcc cggcagaacc cgcccggaag cctcgaggcc cgggtcgccg
     1141 acatgtaccg cgcctatctc gacaccgagg cgatcaaccg cgccggcctc gccccggtca
     1201 agccctatct cgaccggatc gcggcggtga ggagccacga cgagctcgct tcgctctggt
     1261 cggtcaccgg ctatcccgcg ccgatgggca cgttcgtcag catcgaccgc ggcgacccca
     1321 accgcaacat cctctattac gggatcggcg ggatcggcct tcccgaccgc gacaattacc
     1381 tcgtcgacaa cccccgcaac cttgagatgc gcggcaagta tctcgactac atgaccttcc
     1441 tgctcggcaa ggccggccac gccgcgccgc gggccgcggc cgagagcgtc tacgcgctcg
     1501 agaagaagat cgcggcggcg atgtgggacc gggcgctctc ccgcaatccc gagctcacca
     1561 ccaaccggat gtcccatgcc gagctggtcg agctcgcccg gcccttcccg atcgagcgct
     1621 acctcgccgg tcgccagatc ctcgccagcg accgcttcaa cgtcatcaac gtcccgccca
     1681 gcgccgacaa gatccggtcg cagaacctga cgcccgagca gctcgccaag ctcggcaccg
     1741 gccttccggc gatgatgaat ctcatcatgg cgaccccgct cgacacctgg aaggcgtgga
     1801 tgagcgtccg gctgctgtcc ggcgcggccg acctccttcc gagcgacatc gacgacgccc
     1861 agttcgcctt ctacggccag tatctccagg gccgcgagcg tcagcgcgac cgctggcagc
     1921 gggcggtcac cgaagtcgag ggaagcctcg gcgaggcggt cggcaaggtc tatgtcgagc
     1981 gccatttccc gccgtcgagc aaggcgtcga tggaggagct ggtcgccaac ctcaggctcg
     2041 ccatgaccga gaatctcaag gacctcgcct ggatgacccc ggccacccgg accgcggcca
     2101 aggccaagct cgacgcgctg agggtcaaga tcggctatcc ggccgagttc gagacctatg
     2161 acggcctcgc cgtcaccgcc ggcgatccga tcgccaaccg cctctccgcc gcccgctggg
     2221 cctgggcgga ccagctcgac gagctgagga agccggtcga ccgcgagaag tggctgatga
     2281 ccccgcagac ggtgaacgcc tattacatgc cgaccgccaa cgacatggcc tatccggccg
     2341 gctacctgca gccgcccaat ttcaacgcct cggccgaccc ggcggtcaac tacggggcga
     2401 tcggcgccac catcggccat gagatcggcc acggcttcga cgacaacggc tcgcgctacg
     2461 acggcaccgg ggcgctgagg aactggtgga ctcccgagga cgcggccacc ttcaggaagc
     2521 tcggcgagcg gctggaggcg cagtacaacg ccatctgccc gctcgacgaa ggcaagaagt
     2581 gcctcaacgg caagctcacc ctcggcgaga acctgggcga cctcggcggc ctctccatgg
     2641 cctacaaggc ctatcagatc tcgctcaagg gcaagaaggc gccggtcatc gacggcctca
     2701 ccggcgacca gcgcttcttc atctcctacg cccagcacta ccggacgatc taccgcgagc
     2761 ccttcctgcg ccagctgatg gagaccgatc cgcacagccc gagcttcgcg cgggtcaatg
     2821 cggtgctgag gaacttcgac cccttctacg cggcgttcaa ggtgaagccg ggcgacaaga
     2881 tgtacctgcc gccggcggag cgggtgaggg tgtggtgagg gaaggggcgc cgctggcctt
     2941 ccgagggtga cgcggtcata aggcgcaatg ctc
//
LOCUS       DASVQZ010000029         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_392060, whole genome shotgun sequence.
ACCESSION   DASVQZ010000029 DASVQZ010000000
VERSION     DASVQZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392060"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VF547_00770"
     CDS             <1..968
                     /locus_tag="VF547_00770"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX8261386.1"
                     /translation="IDPADSASVAANSLDLTREGVRGIDRMGGTILHTSRTDPRTLAE
                     GDQTARVLEVLGRLGIDAMITLGGDGTLRFSAHLSAQGMPVISIPKTMDNDVFGTDYC
                     IGFSTAVSRSVEAINALRTTVESHERIGVVELFGRRSGETALLAGFLAQVDRTVIAEV
                     PADLGILLPLLAEDKASNPASYAMCVVSEGATVKGAQNGDSELSKPASERGDLGIGQQ
                     LGAAIKSRLGCGTVIQELAYLMRAGEPDAMDRMVGFAFGGLAVQLLQRGEAGRMVTLT
                     RGNYSHVPIATLLQGRKSVDVNGLYDPLTYRARLMRVEGMPMFLY"
     gene            1019..2278
                     /locus_tag="VF547_00775"
     CDS             1019..2278
                     /locus_tag="VF547_00775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX8261387.1"
                     /translation="MRKYWLLAAGASLVAASPASADTVCEWIDFAQAIQTAAAPPPDA
                     PRTGEHDRAQTQVAIAMFEALNAIDRRFESYLGMPAAGEAGASQDAAAATAAYQVLLA
                     HFPGQKTALDDSYNVAMLAVADAVARDKGKAIGEAAAKLALAAGGFDPGRKPVPYRPR
                     TSAGTWTATQLPVFAPYLTTFKPWILPSYESVRPAPPPALGSERWARDYDEIKRLGAK
                     TGSARTPEQTLMARYRITPNMTPTLRLIADAKGRSAVANARMFALLAMVTDDAGIANA
                     EAKLHYNFWRPITAIRNGSDDGNDATAEDPAWEPLIATPNHPEYPCAHCTFAASTAAV
                     LKSEVGDAPPGGVRVHSRSIPNSIVQVVPSLDDWVREVSLSRMYGGVHYRFSNEAGEE
                     IGRKVAEMALAKVMRPLPARGKGRKKG"
     gene            2477..2563
                     /locus_tag="VF547_00780"
     tRNA            2477..2563
                     /locus_tag="VF547_00780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2511..2513,aa:Leu,seq:gag)
     gene            2689..>4102
                     /locus_tag="VF547_00785"
     CDS             2689..>4102
                     /locus_tag="VF547_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8261388.1"
                     /translation="MGSPVSAPGIGQAARKPALRRLGSALAGLFIALLLLAIALILWP
                     AAGAPLPTPGRDLVIANVRVVDVERGLAGLSTSVTIRGGVITAIGPPAAGHALPVIDG
                     GGGYLVPGFWDMHMHSFQLSPQLHLPLFLANGVTSVRDMMDCPGERDSLIACLADKKQ
                     WTAKAEAGRLASPRFVSIASYYFEDPALTPAEASARAGAYAARGLDELKVYNRLSRPA
                     YFRVAAEARRSGMRLTGHLPKAVALEEALASGHRSFEHAHVLVRHCFARSEDWRAGRL
                     EHMPPTRLARFMVLGHDRDRCRRTYEALRRSGAWLVPTHVTREEDARAGDPAFLQDSR
                     LAYLDPLSRWAFRDDLAGTRAEYPGREGEEALGRYFRLGLRLTGEAHRAGVPILVGTD
                     TALGGFRYHDEMAHLVRAGLSPAAVLRAATLDAARFAGLESSAGSVTVGKRADLVLLD
                     SNPLENIANTRRIRLVLLAGR"
BASE COUNT          597 a         1488 c         1393 g          624 t
ORIGIN      
        1 cgatcgaccc ggccgattcg gcgagcgtcg ccgccaacag cctcgacctg acccgcgaag
       61 gcgtccgcgg aatcgaccgg atgggcggca cgatcctcca cacatcgcgg accgatccgc
      121 gcactctggc cgagggcgac cagaccgccc gggtcctgga ggtgctcggc aggctcggca
      181 tcgacgcgat gatcacgctc ggcggcgacg gcaccctgcg tttctccgcg cacctctcgg
      241 cgcagggaat gccggtcatc tcgattccca agacgatgga caacgacgtc ttcggcaccg
      301 actattgcat cggcttctcg accgccgtca gccgctcggt cgaggcgatc aacgcgctgc
      361 gcacgacggt cgagagccat gagcggatcg gggtcgtgga gctgttcggc cgccgcagcg
      421 gcgagaccgc cctgctcgcc ggcttcctcg cccaggtcga ccgcaccgtc atcgccgagg
      481 ttccggccga cctcggcatc ctcctcccgc tgctggccga ggacaaggcc tcgaacccgg
      541 cctcctacgc catgtgcgtc gtctccgagg gcgcgacggt gaagggagca cagaatggcg
      601 attcggagct gtccaagccg gccagcgagc gcggcgacct gggaatcggc cagcagctcg
      661 gcgcggcgat caagtcgcgc ctgggctgcg gcaccgtgat ccaggagctc gcctatctga
      721 tgcgggccgg cgagcccgac gcgatggacc ggatggtcgg cttcgcgttc ggcgggcttg
      781 ccgtccagct gctccagcgc ggcgaggccg ggcggatggt gaccctgacg aggggcaatt
      841 acagccatgt cccgatcgcc acgctgctcc agggccgcaa gtcggtcgac gtcaacgggc
      901 tctacgatcc tttgacctat cgtgcgcgtt tgatgcgggt cgagggaatg ccgatgttcc
      961 tctactgatt cggctgctat tctccctccg gggataggct tttcggggga tttcacagat
     1021 gcggaaatat tggcttctcg cggccggcgc ctcgctcgtc gccgcttcgc cggcctcggc
     1081 ggacacggtc tgcgagtgga tcgacttcgc ccaggcgatc cagaccgcgg cggcgccgcc
     1141 gcccgacgcg ccgcgcaccg gcgagcacga ccgcgcccag acccaggtcg cgatcgccat
     1201 gttcgaggcc ctgaacgcga tcgaccggcg cttcgaatcc tatctcggaa tgcctgccgc
     1261 cggcgaggcc ggagcctcgc aggacgcggc cgccgcgacc gccgcctacc aggtgctgct
     1321 cgcccatttc ccgggccaga agacggcgct cgacgacagc tacaatgtcg cgatgctggc
     1381 ggtggccgac gccgtcgccc gcgacaaggg caaggcgatc ggcgaagcgg cggccaagct
     1441 cgctctcgcc gccggcggct tcgacccagg gcgcaagcct gtcccctacc gcccgaggac
     1501 cagcgccggc acctggaccg cgacccagct gcccgtcttc gcgccctacc tcaccacctt
     1561 caagccgtgg atcctgccga gctacgaatc ggtgaggccg gcgcccccgc ccgccctcgg
     1621 cagcgaacgc tgggcccgcg attatgacga gatcaagcgg ctcggcgcca agaccggcag
     1681 cgctcggacg ccggagcaga ccctgatggc ccgctatcgg atcacgccga acatgacccc
     1741 gaccctgcgc ctgatcgccg atgcgaaagg ccgttcggcg gtcgcgaacg cccgcatgtt
     1801 cgcccttctc gccatggtca ccgacgacgc cggaatcgcc aatgcggagg ccaagctcca
     1861 ttacaatttc tggcggccga tcacggcgat ccgcaacggt tccgacgacg gcaacgacgc
     1921 caccgccgag gatccggcct gggagccgct gatcgcaacg cccaaccatc ccgaatatcc
     1981 gtgcgcccat tgcacctttg cggcgtcgac cgcggccgtt ctcaagagcg aggtcggcga
     2041 cgctccgccg ggcggagtcc gggtccattc ccgctcgatc cccaacagca tcgtccaggt
     2101 cgtcccgagc ctggacgact gggtgcgcga ggtctcgttg tcgcgcatgt acggcggggt
     2161 ccattaccgg ttctccaacg aggcgggcga ggagatcggc cgcaaggtcg cggagatggc
     2221 gctcgcgaaa gtgatgcggc cgctgccggc cagggggaag gggcggaaga agggataatc
     2281 ccgtagatcc tccccccccc cccggatttc cgggggagcg gaagaacccc tccaccatgc
     2341 ttcgcatggt ccccctcccc ggcaaatgcc ggggaggatt tcatacggcc aagcgcgggc
     2401 attgcctcgc tccgtcccct ttgctagagg ccccgctcct accggccccg gacgacgttc
     2461 cggggggcaa tcacgtgcgg tcgtggcgga actggtagac gcgcagcgtt gaggtcgctg
     2521 tggccgaaag gccgtggaag ttcgagtctt ctcgaccgca ccaattatag cgcaggggcg
     2581 gattttccgc cgggcggacc gcttccccga ccctccagtc ctcgcgcgcg ccgtctccgg
     2641 acggccggaa cggttgcggc tcgggtcgcg agcaggcagg ggggagcgat gggctcgcct
     2701 gtctctgcgc ccgggatcgg ccaagccgcc cgcaagccgg cgctgcggcg cctgggctcg
     2761 gctcttgcgg gcctgttcat cgcgcttctc ttgctggcga tcgcgctcat cctgtggcct
     2821 gcggcgggcg ctcccctgcc cacgcccggc cgggacctgg tcatcgccaa cgtccgggtc
     2881 gtcgacgtcg agcgcggcct cgccgggctc tcgacgtcgg tgacgatccg cggcggcgtg
     2941 ataaccgcga tcggaccgcc ggccgccggt cacgcactgc cggtgatcga cggcggcggc
     3001 ggatatctcg tcccgggatt ctgggacatg cacatgcaca gcttccagct ctcgccccag
     3061 ctgcacctgc cgctcttcct cgccaacggc gtcacgtcgg tgcgggacat gatggactgc
     3121 ccgggagagc gggacagcct gatcgcctgc ctggccgaca agaagcaatg gaccgcgaag
     3181 gccgaggccg ggcggctcgc gagcccccgc ttcgtcagca tcgcgagcta ttatttcgag
     3241 gacccggcgc tgacgccggc cgaggcctcg gcgcgggcgg gcgcctacgc cgcgcggggc
     3301 ctggacgagc tgaaggtcta caaccggctt tcgcggcccg cctatttccg ggtcgccgcg
     3361 gaggcgcgca gaagcgggat gcggctgacg ggccatcttc ccaaggccgt ggccctcgag
     3421 gaggccctgg cctcggggca ccgaagcttc gagcatgccc atgtgctggt ccgccactgc
     3481 ttcgcgagat cggaggactg gcgggccggc cggctcgagc acatgcctcc gacccggctc
     3541 gcccgcttca tggtgctggg ccatgaccgc gaccgctgcc ggcggaccta cgaggccctc
     3601 cgccgctccg gcgcctggct ggtgcctacc cacgtcacgc gtgaggagga cgcccgcgcc
     3661 ggcgatcccg ccttcctcca ggactcgcgg ctcgcctatc tcgacccgct gtcgcgctgg
     3721 gccttccgcg acgacctcgc ggggacccgg gccgaatatc cgggccggga gggagaggag
     3781 gcgctgggcc ggtatttccg gctcggtctg cggctgaccg gcgaggcgca ccgcgccggg
     3841 gtgccgatcc tcgtgggcac cgacacggcg ctgggcggct ttcgctatca tgacgagatg
     3901 gcgcacctcg ttcgggcggg cctcagcccg gccgcggtcc tgcgcgcggc gactttggat
     3961 gccgcccgtt tcgcggggct cgaaagcagc gccggctcgg tgaccgtcgg caagcgggct
     4021 gatctggtgc tgctcgactc caatccgctc gagaacatcg cgaacacgcg ccgaatccgt
     4081 ctcgtcctgc tcgccggccg gg
//
LOCUS       DASVQZ010000030         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_200725, whole genome shotgun sequence.
ACCESSION   DASVQZ010000030 DASVQZ010000000
VERSION     DASVQZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200725"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VF547_00790"
     CDS             complement(<1..283)
                     /locus_tag="VF547_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261389.1"
                     /translation="MRSFLIPASLLVAAPVSAKPLVPLAPPVGAAVHSIVAGPRSVPD
                     DHPLRGRIAVEPVAAMPNRVGTLFVSVAKPAEFDTALKLALEQSRMLAGP"
     gene            complement(491..931)
                     /locus_tag="VF547_00795"
     CDS             complement(491..931)
                     /locus_tag="VF547_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261390.1"
                     /translation="MSFSGPVSYFRIAEGEPLPDLKPLAPYRAVVVLAADHGSAWQSE
                     VSDWLVRSGCLYMMAWGPDCSSWDDSVEWSNIMEFDPEDIPEDRFVMTTWHEDEPLDE
                     VFWFAGFCASHGVVEIERTVILHVSLQERGEEMLSRFEAAQSRY"
     gene            complement(928..1842)
                     /gene="era"
                     /locus_tag="VF547_00800"
     CDS             complement(928..1842)
                     /gene="era"
                     /locus_tag="VF547_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX8261391.1"
                     /translation="MANPAPLTPTRCGLVAVVGAPNAGKSTLVNALVGQKVAIVSPKA
                     QTTRTRLMGIAIEGGAQILLVDTPGIFEPRRRLDRAMVAAAWAGAADADLIALVVDSK
                     TGDSRRLSAMLEALQGRREPKILILNKVDLVRKEDLLALASQLTLRGTFDNIFMISAA
                     TGDGVGDLKAALAARVPEGPWHFPEDQVSDASERLAASEVTREQLYLQLHAELPYASA
                     VETEKYEERKDGSVAIHQQILVGRPTQRAIVLGKGGARIKAIGEAARKELEALLERKV
                     HLFLHVKVKPDWEEDRGLYREIGLDWVE"
     gene            complement(1995..2660)
                     /gene="rnc"
                     /locus_tag="VF547_00805"
     CDS             complement(1995..2660)
                     /gene="rnc"
                     /locus_tag="VF547_00805"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX8261392.1"
                     /translation="MDKATLDWLTQLLGAAPGDCDLFERALTHSSYGGDQYERLEFLG
                     DRVLGLVVASWLYALFPDEPEGQLSKRLNALVARDVCAEVARELDVGSHVRLGKQARE
                     DGASDSDNVIGDVVEALIGALYLDSGLAAAEAFVRRAWGDRVSTRDKAPQHPKSALQE
                     WAAAKERKPPAYRLTERSGPQHAPTFVVEVEIRGVGSASAEGASKQEAETAAAAKLLE
                     QLR"
     gene            complement(2787..>3443)
                     /gene="lepB"
                     /locus_tag="VF547_00810"
     CDS             complement(2787..>3443)
                     /gene="lepB"
                     /locus_tag="VF547_00810"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8261393.1"
                     /translation="IGDYLFVAKWPYGYSRYSVPFGLASFGGRIWAGEPERGDIVVFR
                     YPGENEDWVKRLIGLPGDRVQMRRGRLFLNGKPVPKVRIADWLMPVTPNSPCRFGGDI
                     RPPSVEAGVAVCRYPRFRETLPGGRSYDVLDQVDGRADDTPAYVVPAGHYFMMGDNRD
                     DSSDSRFSLEQKGVGLLPRDYVVGRALITFFSTDGSAEWIKPWTWFSAARWERVGGTF
                     "
BASE COUNT          536 a         1194 c         1206 g          507 t
ORIGIN      
        1 ccggaccggc gagcatcctc gactgttcga gcgcgagctt gagggcggtg tcgaactccg
       61 ccggcttggc caccgaaacg aacagggttc cgacccggtt cggcatcgcg gcgaccggct
      121 ccaccgcgat ccggccgcgg agcggatgat cgtcggggac ggaccgcggg cctgcgacga
      181 tcgagtggac ggcggcaccg acgggcggag ccagcggcac cagcggcttg gccgaaacgg
      241 gcgcggcgac gaggagcgaa gcagggatca gaaaggagcg catcctccgg gattagcccg
      301 cgggcgttaa atccccgtga agcctcgcaa gtcggagcgt ctggggcgct cggcagccgg
      361 atcgcgccgt cttgcgttcg ggctgagctt gtcgaacccg tctacctttc ctctcatgga
      421 acgacaggaa agcccttcga caggctcagg gcgaacgggt gggagtggtt cgagccaatc
      481 cctcaaggct ctagtagcgg ctctgcgccg cctcgaaccg gctgagcatc tcctccccgc
      541 gctcttgcaa gctcacgtgc aggatcacgg ttcgctcgat ctcgaccacg ccgtgggacg
      601 cgcagaagcc ggcaaaccag aacacctcgt ccagtggctc gtcctcatgc caggtcgtca
      661 tgacgaagcg gtcctcgggg atgtcctccg gatcgaactc catgatgttg gaccactcga
      721 cggaatcgtc ccagctcgag cagtccggcc cccaggccat catgtacagg caaccggagc
      781 ggacgagcca gtcgctcacc tcgctctgcc aagcggaacc atggtcggcc gcaagcacca
      841 ccaccgctcg atagggcgcc aggggcttga gatcgggaag cggttcgccc tcggcgatcc
      901 ggaagtagct gaccggtccg gagaagctca ctccacccag tccagcccga tctcgcggta
      961 gaggccgcga tcctcctccc agtccggctt gaccttgacg tggaggaaga ggtggacctt
     1021 gcgctccagg agcgcctcca gctccttcct cgccgcctcg ccgatcgcct tgatccgcgc
     1081 gccgcccttg ccgagcacga tggcgcgctg ggtgggccgg ccgaccagga tctgctggtg
     1141 gatggcgacc gagccgtcct tgcgctcctc atatttctcg gtctcgaccg cgctcgcata
     1201 ggggagctcg gcgtggagct ggaggtagag ctgctcgcgg gtcacctcgg aggcggcgag
     1261 ccgctcgctg gcgtcggaga cctggtcctc ggggaagtgc cagggcccct cggggacccg
     1321 cgccgccagc gccgccttga ggtcgccgac gccgtcgccg gtggcggcgg agatcatgaa
     1381 tatgttgtcg aaagtgccgc ggagcgtcag ttgcgaggcg agtgcgagga ggtcctcctt
     1441 gcggaccaga tcgaccttgt tgaggatgag gatcttgggc tcgcggcggc cctggagcgc
     1501 ctccagcatg gccgacaacc gcctcgaatc gccagtcttc gaatcgacga cgagggcgat
     1561 caggtccgca tcggccgccc ccgcccaggc cgccgcgacc atcgcccggt cgagccgccg
     1621 ccgcggctcg aagatgccgg gcgtgtcgac cagcaggatc tgcgccccgc cctcgatggc
     1681 gatgcccatc agccgcgtcc gggtcgtctg cgccttcggg ctgacgatcg ccaccttctg
     1741 gccgacgagg gcgttgacca gggtcgactt gccggcgttg ggcgccccga cgaccgcgac
     1801 caggccgcag cgggtggggg tgagtggggc ggggtttgcc aaatcaggtc cttcgatacg
     1861 ggccccttcg acgccgcctg cggcgtcgct caggacatgc ttcgccaagc tcagtcccta
     1921 ctcaggatga gcggaaaggc acaaccgctc atcccgagca gccggtcagc ccgtcgaacc
     1981 ggcgtatcga aggatcatcg cagctgctcc agcagcttcg ccgccgcggc cgtctccgcc
     2041 tcctgcttgg aggcgccctc ggcgctggcc gagccgacgc ccctgatctc gacctcgacg
     2101 acgaaggtcg gcgcgtgctg cggcccggag cgctcggtca gccggtaggc gggaggcttg
     2161 cgctccttcg ccgcggccca ttcctggagg gccgacttgg ggtgctgcgg cgccttgtcg
     2221 cgggtgctca cccggtcgcc ccaggcgcgc cggacgaacg cctcggccgc ggccagcccg
     2281 gagtcgagat agagggcgcc gatcagcgcc tcgacgacgt cgccgatgac gttgtcgctg
     2341 tcggacgcgc cgtcctcgcg cgcctgcttg ccgagccgca cgtggctgcc gacgtcgagc
     2401 tcgcgggcga cctccgcgca gacgtcgcgg gcgaccaggg cgttgagccg cttggagagc
     2461 tggccttcgg gctcgtcggg gaacagggcg tagagccagg acgcgacgac gaggccgagg
     2521 acccggtcgc cgaggaactc cagccgctca tactggtcgc cgccgtagct ggaatgggtg
     2581 agcgcccgct cgaacaggtc gcagtcgccg ggcgccgcgc cgagcaactg agtcagccag
     2641 tcgagggtcg ccttgtccat ctaccaagcg ccccgacgac gccggggggc ttgccggcga
     2701 ccgccgggac ccgggggttc cgccggcgag gccgggcccc ggccttcgcc ggggagcgag
     2761 cgatcgggcc cgcagcggac cgccatctag aaggtccccc cgacccgctc ccagcgcgcg
     2821 gccgagaacc aggtccaggg cttgatccat tcggccgagc cgtcggtcga gaagaaggtg
     2881 atcagcgccc gcccgacgac ataatcgcgg ggaagcaagc ccaccccctt ctgctcgagg
     2941 ctgaagcggc tgtcggagct gtcgtcgcga ttgtcgccca tcatgaaata atggccggcg
     3001 gggacgacat aggccggcgt gtcgtcggcg cgtccgtcga cctggtcgag gacgtcgtag
     3061 gagcggccgc cggggagcgt ctcgcggaag cgcgggtagc ggcagaccgc gacgcccgcc
     3121 tccacgctcg gcggcctgat gtcgccgccg aagcggcacg ggctgttcgg ggtgaccggc
     3181 atcagccagt cggcgatccg gaccttgggc accggcttgc cgttgaggaa gagccggccg
     3241 cgccgcatct gcacacggtc gccgggcagg ccgatcagcc ttttcaccca atcctcgttc
     3301 tcgcccggat agcggaagac gacgatgtcg ccccgttcgg gctcgccggc ccagatccgg
     3361 ccgccgaacg aggcgaggcc gaaggggacg gagtagcgcg aatagccgta gggccatttc
     3421 gccacgaaca gatagtcgcc gat
//
LOCUS       DASVQZ010000031         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_584663, whole genome shotgun sequence.
ACCESSION   DASVQZ010000031 DASVQZ010000000
VERSION     DASVQZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584663"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VF547_00815"
     CDS             complement(<1..826)
                     /locus_tag="VF547_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8261394.1"
                     /translation="MVLPRLASAALRFANSRLLDLSYRLDLPWALGGPESLMVEPSGA
                     CNLRCPLCPTGLKATKRDGYTLSPDDFERALGWFRFTLRTITFWNYGEPFLNRDLAKM
                     VAIASRNGIRSRISTNGHFLEGPMLDDVLAAGLSQLIVSVDTPDPALYGRYRVRGDFE
                     RVVRGFRHAAARKRALGAGTDIIAQYMLMSGSEDVDAIIAHGEALGADKVVVKTIGIG
                     SSVPQPSDRDWALLPEADEFNRYVSRDDLRAKVNWEDSRCSYIWRRMVLNSDGTCVP"
     gene            complement(901..1059)
                     /locus_tag="VF547_00820"
     CDS             complement(901..1059)
                     /locus_tag="VF547_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261395.1"
                     /translation="MSRRDNSDSDGLTPATEQKKPWIAPTAEAQPVRAITAGLSSPNT
                     DGTSGCHS"
     gene            complement(1119..>1619)
                     /locus_tag="VF547_00825"
     CDS             complement(1119..>1619)
                     /locus_tag="VF547_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261396.1"
                     /translation="HSGAGKSTTAYELVRRGWTIVSDDLTRVSLEKGVPLAWPGRSRL
                     RLLPDACRDFGLDPAELASAPKRLGKYIVDLPRWQDPIALSAVVALERSEAALEVLAV
                     SGAARTAVLAEHTYRPHYVAALGRTVEHFRLVAATAGFTKVLRLQGAASVREVADVVA
                     AAACEH"
BASE COUNT          253 a          536 c          588 g          242 t
ORIGIN      
        1 aggggacgca ggtgccgtcg gaattgagca ccatccgccg ccagatgtag gagcagcgcg
       61 aatcctccca gttgaccttg gctcgaaggt cgtcgcgcga gacgtatcgg ttgaactcgt
      121 ccgcctcggg cagcagcgcc cagtcgcggt cgctcggctg cggcaccgac gagccgatcc
      181 cgatcgtctt caccaccacc ttgtcggcgc cgagcgcctc gccatgggcg atgatcgcat
      241 cgacgtcctc ggagccgctc atcagcatat attgggcgat gatgtccgtc cccgcgccga
      301 gcgcccgctt gcgggcggcg gcatggcgga agccgcgcac cacccgctcg aaatccccgc
      361 gcacccggta gcggccgtaa agcgcggggt cgggcgtatc gaccgagacg atcagctggc
      421 taagcccggc ggcgaggacg tcgtccagca tcggcccctc gaggaagtgg ccgttggtcg
      481 agatccggct gcggatgccg tttcgcgagg cgatggcgac catcttcgcc aggtcccggt
      541 tgaggaaggg ctcgccgtaa ttccagaagg tgatcgtgcg cagagtgaag cggaaccagc
      601 cgagcgctcg ttcgaaatcg tcgggtgaga gcgtgtagcc gtcgcgcttc gtcgccttga
      661 gtccggtcgg gcagagcggg cagcgcaggt tgcacgcccc gctcggctcg accatcagcg
      721 attccggtcc gcccagcgcc caggggaggt cgagccggta ggagaggtcg agaagcctcg
      781 aattggcgaa gcggagcgcg gcggaggcga gacgtggcag gaccatgggc cggtctcctc
      841 gtcgggccga aggcggctcg cggccccggg ccgccggaag agccgtcggc cggcccgcgc
      901 ctaggagtgg cagccgctgg tgccgtccgt gttggggctc gacaagccgg cggtgatggc
      961 ccttaccggc tgggcctccg ctgtcggcgc gatccagggc ttcttctgct cagtcgcggg
     1021 cgtcaggccg tcggagtcgc tgttgtcgcg tctgctcatg tcttgctccc gtcggaaatc
     1081 acagtggccg gcgttatggc cgcttcgaag cctgtgcgtc aatgctcgca agcagcggcg
     1141 gccacgacgt cggcgacctc ccgcaccgat gccgccccct gcagccggag gaccttggtg
     1201 aagccggcgg tggcggcgac gagccggaag tgctccacgg ttcggccgag cgcggccaca
     1261 taatggggcc ggtaggtatg ctccgcgagg accgccgtcc gtgcagcccc cgaaacggcg
     1321 agcacctcca acgcggcttc ggaccgctcg agcgcgacga ccgcggaaag ggcgatcgga
     1381 tcctgccacc gaggcaggtc gacgatatat ttgccgagcc gcttgggcgc cgaggcgagc
     1441 tcggccgggt cgagtccgaa atcgcggcag gcgtcgggca ggaggcgcaa tcgggaacgg
     1501 ccgggccagg cgagcggaac gcccttctcc agggacaccc gggtgagatc gtcgctgacg
     1561 atggtccagc cgcggcggac cagctcatag gccgtcgtcg acttgccggc gcccgaatg
//
LOCUS       DASVQZ010000032         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_873784, whole genome shotgun sequence.
ACCESSION   DASVQZ010000032 DASVQZ010000000
VERSION     DASVQZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873784"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VF547_00830"
     CDS             <1..1511
                     /locus_tag="VF547_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8261397.1"
                     /translation="GIGSAAGLGFVAVSGAWARGDGFVPIVVAHRGPADRPSPYEQSS
                     LAVRGIAPLSGGTELQASALLFSDERERGTAFSEIRTRGADASLRVVGRRWSALAYLQ
                     ARDFYNSFASVNPARTTATRTAEQHDVPATGLGARVEARPDVGRGVELRLGGDWRRTE
                     GETRERFAFVAGSGTRGRVAGGRTQTFGAFAEAAWTAGSLTLTAGGRADRWSIADGRL
                     HERVLATGAVLTDTAFRDRSGWEPTGRAGIAWRADSGLTLRSAAYLGWRLPTLNELYR
                     PFRVGVDATAANSELAPERLKGVEAGLEWRPAPRAGIDLTLFANRLDGAIANVTLGRG
                     PGTFPGVGFVAAGGDYRRRANLDSISAKGVELDLRYARGQWSLSGGWSWVEAKVEASG
                     AASALDRLRPAQTPRHSISTTLAWTGHRARAAVSARYVGGQFEDDLNRQRLPGAITLD
                     AAGSWKLSPALALEARAENFGGARVLAGISGDGVEERATPRTLWIGLGVRIR"
     gene            complement(1512..2252)
                     /locus_tag="VF547_00835"
     CDS             complement(1512..2252)
                     /locus_tag="VF547_00835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8261398.1"
                     /translation="MTAQWKGAHPRNFSRGRSGHRPEAIVIHIMDGTLVGTDSWFNDP
                     VSRVSAHYGVGKTGIVHQYVRETDSAFHAGTIVAPTWRLLKRDVNPNFYTLGIEHEGF
                     GGRGAAWPEAMLEASLALVRDLASRWDIPIDADHIVPHRAIRANKPNCPGIDIADYVE
                     RLAAIAPPPRPAPVETAFARQVRIVRAVNVRRGPTTAGVALRTLLPGDAFTAVAKTVG
                     EEVSGNDIWFRNAGQEYVWAANTDHPNG"
     gene            complement(2264..>3103)
                     /locus_tag="VF547_00840"
     CDS             complement(2264..>3103)
                     /locus_tag="VF547_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261399.1"
                     /translation="AVTALVLAVVLQLDSIGLINRLSVDEELRNSLVEQAVKNQEAWR
                     LKMLALEQQQQQQQLEQQQQQQQPLLPQKSVAEPQSSGAGDTADEGDFGLNLVRAKAV
                     EGNATAGEGNASTAEDNASTAEGNAATAEGKVAAARDDAVETNTVANKKRKPEEEPLV
                     AVRRTIEQVGVVALPRDWKQWSEAWWEPAEHEGGFRWALLFGILLSAALLSLGAPFWY
                     SVLANLLKLRSALARKDEVQREERQTTQAAATATTTGAPAVAGGLPPNLAGGEAGDLG
                     ATG"
BASE COUNT          400 a         1085 c         1126 g          492 t
ORIGIN      
        1 ccgggatcgg atcggcggcc ggccttggct tcgtggcggt ctcgggggcc tgggctcggg
       61 gcgacggctt cgtcccgatc gtcgtcgccc accgcgggcc ggccgaccgg ccctcgccct
      121 acgagcagtc gagcctggcc gtgcgcggga tcgcgcccct ttccggcggg accgagctgc
      181 aggcgagcgc gcttctgttt tcggacgaac gggagcgcgg caccgccttt tccgagatcc
      241 gcacgagggg cgcggacgcg tcccttcgcg tcgtcgggcg gcggtggagc gcgctcgcct
      301 atctgcaggc ccgcgacttc tacaacagct tcgccagcgt caatccggcg cggaccaccg
      361 ccacccggac ggcggaacaa catgatgtgc ccgcgaccgg cctcggcgcc cgggtcgagg
      421 cgcggccgga cgtcggacgc ggagtggagc ttcgccttgg cggcgactgg cggcggaccg
      481 aaggcgagac ccgtgagcgc ttcgccttcg tcgccggctc gggcacgcgc ggccgggtcg
      541 ccggtgggag gacgcagacc ttcggcgctt tcgccgaggc ggcctggacc gccgggtcct
      601 tgacgctcac ggcgggcgga cgggccgacc gctggagcat cgccgacggc cgcctgcacg
      661 agcgggtgct ggcgaccggg gcagtgctca ccgacaccgc gtttcgcgac cgttcgggct
      721 gggagccgac cggccgggcc gggatcgcct ggcgggcgga ctcgggcctg acgctgcgca
      781 gcgccgccta tctgggctgg cgcctgccga ccctcaacga gctctaccgg cccttcaggg
      841 tgggcgtcga cgcgacggcg gccaattccg agctcgcgcc ggagcgactc aagggcgtcg
      901 aggccgggct cgaatggcgg ccggcgccgc gggcggggat cgacctcacc ctgttcgcga
      961 accggctcga cggcgcgatc gccaacgtca cgctgggacg cgggcccggc acctttcccg
     1021 gggtcggctt cgtcgccgcc ggcggcgact accgccgtcg cgcgaatctc gactcgatct
     1081 cggcgaaggg cgtcgagctg gacctgcgct acgcccgcgg ccaatggagc ctgtcgggcg
     1141 gctggtcgtg ggtcgaggcg aaggtagagg cgagcggcgc ggcctcggcg ctggaccggc
     1201 tcaggcccgc gcagactccc cggcacagca tttccacgac cctcgcctgg accgggcatc
     1261 gcgcccgcgc cgccgtcagc gcccgctatg tcggcggcca gttcgaggac gatctcaacc
     1321 gccagcggct gcccggggcc atcaccctcg atgccgccgg ctcctggaaa ctgtcgccgg
     1381 ccctggcgct ggaggcgagg gcggagaatt tcggcggagc tcgggtgctc gcgggaatca
     1441 gcggcgacgg ggtcgaggag cgggcgacgc cgaggaccct gtggatcgga ctgggggtcc
     1501 ggattcgctg atcatccgtt gggatggtcg gtattggccg cccagacata ttcctgcccg
     1561 gcgttccgga accagatatc gtttccggac acctcttccc cgaccgtctt ggcgaccgcg
     1621 gtgaacgcgt ctccggggag caaggtgcgc agcgcgaccc ccgccgtggt cgggccgcgc
     1681 cggacgttga ccgcccggac gatccgcacc tggcgcgcga aggcggtctc gaccggggcc
     1741 ggtcgcggcg gcggcgcgat ggcggccagc cgctcgacat aatcggcgat gtcgatgcca
     1801 gggcaattgg gcttgttggc gcgaatcgca cggtgcggga cgatatggtc cgcgtcgatc
     1861 ggtatgtccc agcggctcgc caggtcgcgc accagcgcca ggctggcctc gagcatcgcc
     1921 tccggccagg cggcgccgcg gcctccgaag ccctcatgct cgatcccgag cgtgtagaaa
     1981 ttggggttca cgtcgcgctt cagcagccgc caggtcgggg cgacgatggt tccggcatgg
     2041 aaggcgctgt ccgtttccct gacatattga tggacgatcc cggtcttgcc gaccccgtaa
     2101 tgggccgaca ctcgcgagac cggatcgttg aaccaggaat cggtgccgac gagggtgccg
     2161 tccatgatgt ggatgacgat cgcttccggg cggtggccgg agcggcccct gctgaagttg
     2221 cgggggtggg cgcctttcca ttgcgcggtc atggcggtct ccctcagccc gtggcgccga
     2281 ggtcgccggc ttcgccgccc gcgaggttgg gcgggagtcc gccagcaacg gcgggcgccc
     2341 cggtcgtcgt cgccgtcgcc gcggcctggg tcgtctggcg ctcctcgcgc tgcacttcgt
     2401 ccttgcgggc gagcgccgag cgcagcttca ggaggttcgc gagaaccgaa taccagaagg
     2461 gtgcgccgag gctgagcagg gccgccgaca gcaggatccc gaacagcagc gcccagcgga
     2521 agccgccctc gtgctcggcc ggctcccacc acgcttcgct ccattgcttc cagtcgcgcg
     2581 gaagggcaac gacgcccacc tgttcgatcg tccgccggac ggcgacgagc ggctcctcct
     2641 ccggcttccg cttcttgttc gcgactgtgt tggtttccac cgcatcatcc cgagcggcgg
     2701 caaccttgcc ctcggccgtg gcggcattgc cctccgccgt ggaggcattg tcctcggcgg
     2761 tggaggcatt gccttcgcca gcggtggcat tgccttcgac cgctttggcc cggaccaggt
     2821 tcaggccgaa atccccctcg tcggcggtgt ccccggcacc gctggactga ggctctgcga
     2881 cagatttttg cggcaacagc ggctgctgct gctgctgctg ctgctccagt tgctgttgct
     2941 gttgttgctg ttcgagggcc agcatcttca ggcgccaggc ttcctggttc ttgaccgcct
     3001 gctcgacgag gctgttgcgc aactcctcgt cgaccgaaag gcggttgatc aggccgatcg
     3061 aatcgagctg gaggacgacc gcaagcacca acgcggtcac cgc
//
LOCUS       DASVQZ010000033         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_393122, whole genome shotgun sequence.
ACCESSION   DASVQZ010000033 DASVQZ010000000
VERSION     DASVQZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393122"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="hisB"
                     /locus_tag="VF547_00845"
     CDS             <1..933
                     /gene="hisB"
                     /locus_tag="VF547_00845"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX8261400.1"
                     /translation="RALPPYPLPSLSIEAALAALAPSRRPVHLERIARIKAERERLAP
                     LLARSPVVKSVRSGGGNFLFLEVDEPEALAAKLHGLGIRMRFRPNAAPGGLRLTIGTE
                     AENEAALAALGVSGGRAAHRRADLVRDTKETKIAVAVDLDAPEPRRIATGIPFYDHML
                     DQVAAHAGFSLLLSCDGDLDIDGHHSIEDCAIVLGSALSRALGDRRGIGRFGFSLPMD
                     ETEAHVLIDLSGRPYSRFEGSFGAGQIGAYPTEMTRHVFRSLADSLGAAIHVRVEGEN
                     DHHKTEACFKAFGRALRQALARHGDVLPSTKGVL"
     gene            930..1511
                     /gene="hisH"
                     /locus_tag="VF547_00850"
     CDS             930..1511
                     /gene="hisH"
                     /locus_tag="VF547_00850"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX8261401.1"
                     /translation="MKLAIVDVGCGNIGSVGLAFQRFGIDPTVTGDPAEIAAADKVVL
                     PGVGAAGYAMEQIEARGLAPVLRELRQPLLGICLGMQLLFESSEEEETDCLGLIGGRV
                     RRLDAGPGRPVPHMGWSRLEVGDEAIGLVTGDYVYFAHSFACDSGPETVASADYGREI
                     PAVVRSGNRLGAQFHPERSGEAGARFLRCWLGR"
     gene            1508..>1973
                     /locus_tag="VF547_00855"
     CDS             1508..>1973
                     /locus_tag="VF547_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX8261402.1"
                     /translation="MILYPAMDLMDGRIVRLAQGRFEDSTDYPADPAEALARFAAAGA
                     RWAHVVDLSGTRAGAPAQHGLLADLARGAPLKLQVAGGFRTAEQMRALYEAGVARIVI
                     GSLAVDDPGAVQAFIDAFGAERVALALDVKVEGGEPIVHTRGWAHRTGLTLWE"
BASE COUNT          264 a          707 c          711 g          291 t
ORIGIN      
        1 cgggcgctgc cgccctaccc gctgccgagc ctgtcgatcg aggcggcgct tgcggcgctc
       61 gccccgtcgc ggcggccggt gcacctggag cggatcgcgc ggatcaaggc ggagcgcgag
      121 cggctcgccc cgctcctcgc ccgctcgccg gtggtgaaga gcgtccgcag cggcggcggg
      181 aatttcctgt tcctcgaggt ggacgagccg gaggcgctgg cggcgaagct gcacggcctc
      241 ggcatccgca tgcgcttccg ccccaatgcc gccccgggcg gactccggct caccatcggc
      301 acggaagcgg agaacgaggc tgcgctggcg gcgctcggcg tctccggcgg ccgcgccgcc
      361 caccgccggg ccgacctcgt ccgggacacg aaggagacga agatcgccgt tgccgtcgat
      421 ctcgacgcgc cggagccgcg gcggatcgcc accggaatcc ccttctacga ccacatgctc
      481 gaccaggtgg cggcgcatgc cggcttctcg ctcctcctct cctgcgacgg cgatctcgac
      541 atagacggcc accacagcat cgaggattgc gcgatcgtgc tcggctcggc gctgtcgcgt
      601 gcgctcggcg accgccgcgg catcggccgc ttcggcttct ccctgccgat ggacgagacc
      661 gaggcgcacg tcctcatcga cctgtccggc cgtccctaca gccggttcga gggaagcttc
      721 ggggccggcc agatcggcgc ctatccgacc gagatgaccc gccacgtctt ccggagcctc
      781 gccgacagcc tgggagccgc gatccacgtc cgggtggagg gcgagaacga ccatcacaag
      841 accgaagcct gcttcaaggc cttcggccgg gcgctgaggc aggcgctcgc ccggcacggc
      901 gacgtgctgc cgagcaccaa gggggttttg tgaagctcgc aatcgtcgac gtcggctgcg
      961 gcaatatcgg ctcggtcggg ctcgccttcc agcggttcgg gatcgatccg actgtgaccg
     1021 gcgatcccgc ggagatcgcc gccgccgaca aggtggtcct gcccggagtg ggcgcggccg
     1081 gctatgcgat ggagcagatc gaggcgcgcg ggctcgcgcc ggtgctccgc gagcttcgcc
     1141 agccgcttct cgggatctgc ctcgggatgc agctcctctt cgagtcgagc gaagaggagg
     1201 agacggattg cctcggcctc atcggcggcc gggtgcgcag gctcgatgcc gggcccggcc
     1261 ggcccgtccc ccacatgggc tggagccggc tcgaggtcgg cgacgaagcg atcggcctgg
     1321 tgaccggcga ctatgtctat ttcgcccaca gcttcgcctg cgattccggg cccgagaccg
     1381 tggcgagcgc ggattatggg cgcgaaatcc cggcggtggt ccggagcggt aaccgcctgg
     1441 gagcgcaatt ccatcctgaa cggtcgggcg aggcgggcgc ccgcttcctc cgctgctggc
     1501 tcggccgatg atcctctatc ccgcgatgga cctgatggac ggccggatcg tccggctcgc
     1561 ccagggccgg ttcgaggaca gcacggacta tcccgccgat cccgccgaag cgcttgcccg
     1621 cttcgccgcc gcgggcgccc gctgggccca tgtcgtggac ctcagcggca ctcgcgccgg
     1681 ggcgccggcg cagcatggcc tgcttgccga tctggctcga ggcgcgccgc tgaagctcca
     1741 ggtcgccggg ggattccgga cggcggagca gatgcgggcg ctctacgagg cgggagtcgc
     1801 ccggatcgtc atcggaagcc tcgccgtgga cgatcccggc gcggtccagg ccttcatcga
     1861 cgcgttcggc gcggagcggg tcgcgcttgc gctggacgtc aaggtcgagg gcggcgagcc
     1921 gatcgtccac acccggggct gggcccatcg caccggcctg accctttggg aag
//
LOCUS       DASVQZ010000034        20849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_201698, whole genome shotgun sequence.
ACCESSION   DASVQZ010000034 DASVQZ010000000
VERSION     DASVQZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 20849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20849
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201698"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VF547_00860"
     CDS             complement(<1..440)
                     /locus_tag="VF547_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261403.1"
                     /translation="MPPFRFDPRLSLGSFCAALAAFAASSAAAAGEPAPIIRVETGAH
                     QAFINRLVVLDEGRLLTVSDDQTARSWNHKGRLLAVARGRVGPRDEGALYAAAVSPRY
                     VALGGRAGAAEGAPFVRLLDRATLKPAGLLADLPDVVTALAFST"
     gene            complement(421..1248)
                     /locus_tag="VF547_00865"
     CDS             complement(421..1248)
                     /locus_tag="VF547_00865"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX8261404.1"
                     /translation="MGAGAGLLLTKGSTEGALKGALVGVAAGAGSAYLESKKRANSDR
                     RALAGSVYEDVANANAEAARGLRAFSQARNCRVTEALRIKAAVGRGELSREAATSQLA
                     EHKKRLERDIKLAEKFAKSYDDALVQFRSAAGFLAEGRPDDQLYLEQFAPAKAAAAPP
                     PAILTAKTALTVRSAASVKAAAVSKLARGEAVELLSAQPGGWHEIRRSDGSKGFVAAR
                     FLTASASTGPARVNLSRVSPEVRTVAQPMFEGMEKRGALDAELRVARANTSNATFSL"
     gene            1625..2053
                     /locus_tag="VF547_00870"
     CDS             1625..2053
                     /locus_tag="VF547_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261405.1"
                     /translation="MHKLNLALIAGLGLIASSPALAQDDPDTTINGDTTINASLKNSA
                     TGAGGEDAIATTDVGTISGSKINGTTEINATAENVVTGALGEGAEARLEVGVINNASI
                     DGNTRINASAENIGTVAIGENACAATRVGAIGTKPCKKPR"
     gene            2050..2394
                     /locus_tag="VF547_00875"
     CDS             2050..2394
                     /locus_tag="VF547_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261406.1"
                     /translation="MTRRRLAAAAAALVAGAALAQVVTAQQLNPRKSAPPAAESKKSR
                     MVARAELQAQQDGGSLKSEELTVTKSCGNVQIGGAPPPDPGKSRTLVGSQPGLRDRDN
                     ATVVSNVVNICR"
     gene            2473..3996
                     /locus_tag="VF547_00880"
     CDS             2473..3996
                     /locus_tag="VF547_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX8261407.1"
                     /translation="MAAAAALLSHAAGGAGWAAPKPGNDPRRAAVAAGPDMRATRTVT
                     NFAEALSCMDDLLLQYGKRDIAIINDGIPDATATLRVGTRDMVISALDAMSLKSGAFK
                     YVDADQSDENVRVMQLNVNGSLQRADYYIKGSISQIDQEVLSSGKRMGIATDFLSVGA
                     SKDRSVTNIALELGMYRTSDRTLIRGVRTQNTIQVVRKGKGMDVGGLLPFASLVYEVH
                     SDRAQGSHQTVRTLVELSLIELVGKFTKVPYWRCLALPSADPMARHQADDYYARMRPP
                     EQIMAAQTALHHLGYYQGQFDGVQSPQLGQAVSRYRAEHGLGSGPHVDFELYFSFLTK
                     GLVANGDAGAKRPRPIMAEPIADDGLRFTMKLSDLVQQGSELKLELTPTRNAYFYCFM
                     AGNGKQAYRIYPNRFSGDQPMTQAGSTLQIPAEGDKLAIRLNDVGIERVACIARTQRY
                     DVPPPSLAANDLSPIVASGKLAGLDSVINEHQKRDSMGLNSSVQVKEIQVTRASASR"
     gene            3993..4571
                     /locus_tag="VF547_00885"
     CDS             3993..4571
                     /locus_tag="VF547_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261408.1"
                     /translation="MIRLAAIGLALVAPASRGSAQDEPDTTVPSAQEAAAHRVADPAA
                     PAAKPARAATRVGVSSGGSTRVDAHARNVMTVADDGVAVTNIGVGRPGASTSVYAEDI
                     VTVGRGQSAETDIGTGSGFVSATDIYNGGGRLSIGAEGTKRDGKTCVEIYRNTCIVHF
                     YYRRKNDPCAPGYWIDGRKCRLPSDRKHRIGS"
     gene            4845..5105
                     /locus_tag="VF547_00890"
     CDS             4845..5105
                     /locus_tag="VF547_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261409.1"
                     /translation="MEAEANAEIAFSLNGLIATLLRIRYEFKRLARFRVSLKERCLVA
                     DINLETQEHDLDDDFVVLDADDFVEVAGGTGGHTQVGSAFKA"
     gene            5336..5992
                     /locus_tag="VF547_00895"
     CDS             5336..5992
                     /locus_tag="VF547_00895"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX8261410.1"
                     /translation="MPLRARQHCLPFSLASGWGYHVHSPINFKAMWDGKQIFWNYGDV
                     KGWLPLNTAYLPNSWQRHSEHAPAGSEEFCPIFLEAFPEPGVMQIWSGYVAQTPQDWS
                     LLVRSPINLPGSSFYEVIEGIIETDWWVGPLVTFIRFTKTDVPVKFRRAVPLFQVAPL
                     PRGAYSFQQPAELVHEEPFSEWSEADWDAFRITFEKRNSGKPGDYARYARKRPPPEAL
                     "
     gene            5989..7260
                     /locus_tag="VF547_00900"
     CDS             5989..7260
                     /locus_tag="VF547_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8261411.1"
                     /translation="MTVDPVAENAGAGKPRLFRAAAMNEFERRGAGEPILQQPPNFTT
                     YLIAISILCAALFAFVALATWPAVHTASGYVIPSRSPIEIYPSGSGVVIAIAAPGSRV
                     RAGQPLARLSADTSSLTGSGTAEARLQALESQRQANSNEIRLVDMDRKSRLAQLDSEQ
                     ALLAARIENLRRQSAIANARIANAEQDVAASRTLVDKGYMAAHEMRRRQNLLLEARQA
                     ITVLDLEAVAARNRLRAIAFEKDSISASFSRQAESLRAESFARSGEIATARLAVGETV
                     VAPVSGRVASNEVQVGQRVDKRARLAILLPEATRLQIELALPSRAAGRIAPGQRVSLD
                     FDSYPAAKYGRQTAVVSSVEPIATRSSAGAIAGARTEPEFRILVRPDRDVIAYDGQTY
                     PLRPGMKVRARIVIERRTLLAWLLSPFKTFG"
     gene            7257..9437
                     /locus_tag="VF547_00905"
     CDS             7257..9437
                     /locus_tag="VF547_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX8261412.1"
                     /translation="MTMGMTDQSPLLFSRRRKVPVIYQLEAAECGLACLAMVCGYYGK
                     HVGIFELRHSFRISTKGATVQSLVQAARSAGLLSRVLKVGMGSIHRLQLPAVLHWNLN
                     HFVVLAERKGGKYLINDPARGAHWLDEEEVSKQFTGIAMELTPQEGFETRKRVPALTF
                     STLLRGVVGARGFFVQLALLSLVLQLLALVSPLAVRLIIDNAAMQGDSEFLLVLAAGL
                     GIVILASIALTVLRSKLIVYFSNQFSYHVAVRVFAHMVALPVAYFQARSYGDIVMRYR
                     AVEPIRDLITKAFIPAVLDGIMGILTLLVLFLLSPLLALIPAAAAAGLAAVYLASAAS
                     SRSFQEARTTTRAIDISRVLELVRSIETVKGFGKEDLTRGHWQNLHAQFINANINVDR
                     LAARLATVKTSATLAEEGLVIVIGAAMILGGSLTVGTLLAFYLLNRFFIEKISQFTEQ
                     FLAFRDTSVQLDQLADITTAEPEQTASRSAGLQDVKGALSVDDVWFRYGDAEEWVLKG
                     ASFEVEPGEHLAIAGASGGGKTTLLKVASGLLMPTSGAVRLDGIPIARIDVASLREAL
                     LCVQQNDHLLTGTIAENISFFSPTLDWDRIVACAKMAYIYDEIMQFPMQFHTFVGDLQ
                     NSISGGQKQRICLARAFYQTPKVLGIDEGTSHLDPRAESAVTRTMSGLGITRITVAHR
                     IETLRTADRILLIEDGKLRPVSLDEIKEIGNGRASRDRAFAGGG"
     gene            9394..10491
                     /locus_tag="VF547_00910"
     CDS             9394..10491
                     /locus_tag="VF547_00910"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8261413.1"
                     /translation="MVAQAGIEPSPAEGELEEERLRLQAIRSAVALAGGEPRGGLAAM
                     PRPAELRVLAASRLDSIAYLQFQPDHPVQQLYDAIWLAQQAVVERFVEAAAAAGLVVL
                     PVQGIEKNARYLRGHSLNRRGDLDFIVRAEDLPALARIAESQGFRQAAIAHEGGFLPL
                     TAEQMGMGLAASGMEYQREYPFHGFMPFVAPERMLARIPQALNPLRRVGSKVELILAL
                     DMMTAYHESAPAPLHWERLRASGFRGAQTQAAELDLLFSAHRLYQKLAVAPGPHVTRM
                     LAELVLVLVAEPDLDWEFLESAAMTCRMGAAAYYGLGTAAALIGHPSLHFIETRLANH
                     ADGSIRDEGWLPARLFPAGLLAAEMPDWLIR"
     gene            10535..12370
                     /gene="asnB"
                     /locus_tag="VF547_00915"
     CDS             10535..12370
                     /gene="asnB"
                     /locus_tag="VF547_00915"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8261414.1"
                     /translation="MCGIAAILSEDLGEAAVEARTLAIRRMVAAQTSRGPDAEGVYSH
                     GPVTMGHNLLRILPCPNSTQPFTSSDGALTICYNGEIYNYLDLRADLIRQGVRFRSDT
                     DTETILELYRRDGLDAFAKLRGMFALVLHDVRNGRVIAARDAMGQKALYMRPSADGLY
                     LASTVAALRAGCSEPAEINRRALHFYLWTLMPPAEETMLTGIELLPRGQAAIFDAGGR
                     LLRMTSFAGADGAGAVEDDLDRAGATLTALLAAAVGEQSEGVPRLATHLSGGLDSSAI
                     LSVLPASLRPDVTSYTCSYKVNSVDVPEEERGFEEAFVARRVADYVGVRNEVVLIGAE
                     EYAANLFDVVATVDEPRGNPCLPHFFLARRVAERHRVVLSGEGADELFGGYPWKTRCA
                     ESPDGSAFLQAMAPAPVELLERSLDGRWADQADVRDYVLSSVAAPTRDERLRKILEFD
                     QAHFLQYLLLQADKLGGRFSIEDRYPFLDRRIVDFARSLSPRLLSGGMANSKPVLRRA
                     LKNLVPDEVLTRPKMGFVAPEGSWYRHFLSPLLRDILLSPDSFVRTFYKTGAVEHILE
                     DHFRGRANYRKLIWSLLTLEIWHRTVIRAEGAVDLQQEYRNGRAA"
     gene            12416..13198
                     /locus_tag="VF547_00920"
     CDS             12416..13198
                     /locus_tag="VF547_00920"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8261415.1"
                     /translation="MIDDSWLLLWSGSATVDSVDQLFSPRDWAAGIDSGELRRVEQDW
                     AAALRKAQEQGRRLFDSPAVRLCGLEVRSGRLQLRYELTRYAYYVGARSRSVPTPFSG
                     NPDPTGTVVIGVTADSKIPIGKRHQDVDANPGKFFCFGGFLHPTEDASAEGIPDAFAC
                     ARREWREETGSDLEGAGLELISIVYDRVYPHPEMAFLARTAATSGEFVASRWDAELSD
                     LSFVPVDGIEEFVRRHRTDFTESLVGAFEVLAKRYRIGRDAD"
     gene            13188..14228
                     /locus_tag="VF547_00925"
     CDS             13188..14228
                     /locus_tag="VF547_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX8261416.1"
                     /translation="MLTEDGFFGGSEFGRAFEADLRAALAPEQLAMLSQLCFVLIKPD
                     ALLSGKAGEILDRLRASGFEIVFAHLLQAPRESQFEDLYKYNLTLYNVKHQLGAWWLN
                     RQIYGLGPSLSLLVRGGDGTAGDIAARLGRLKGPSSPYNGAPGQIRWDLQATNRSLNL
                     VHSADGPLSTAREFQLFHPPEALKAVLDSMIEGALHTAHSKAGRMVLAFRASRQEFSF
                     LPTLLALKARVAIAGYGDSNEAATARLLDLYSSIGAASSMTEEVRSILRLAEREREVA
                     GALWPADGPLDQLFRLLAAPERYGPALAAALSHRLKACQIHVSGIELLVLQTSIYYFA
                     ADALAAEAHSPA"
     gene            complement(14231..15358)
                     /locus_tag="VF547_00930"
     CDS             complement(14231..15358)
                     /locus_tag="VF547_00930"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8261417.1"
                     /translation="MPEALSDADERRNENLLLLASGLLGSGRERLCAAAERLIEGIDP
                     LPSSVSPYLLQRAEQTRLVPALDHAAERMGLSGREPLQALSGVHAAAQRRLFEEAGAP
                     LDALEARGIAPALIKGSDYVWSLYPRSRPRQMADVDLLVKPQFLASTIEAFTELGFRQ
                     GAFNRDTLVLTELTSTEFENFLKEHYEMPPFLKLVRCPELDAQRSIINSYCVPRDYIL
                     TCGEEVWLLIEIDVHYNLSFDLSLDVVWRGGREVQAWGRTRRGLAWETQIWFLAARLY
                     VEATDQKQPVLRSLVDLAAILDAAPAIDWDWVIEVGELYGLFVAFHYVFEVLRQLDPA
                     LVPESVARRCLAEATAARNERVNRGALLPALLDSLETQRLA"
     gene            complement(15407..15760)
                     /locus_tag="VF547_00935"
     CDS             complement(15407..15760)
                     /locus_tag="VF547_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261418.1"
                     /translation="MEKNAPAVKEAQTPLDYLASAAARIGSASAELGPRLRSVSKPDD
                     LAAHAQWVASAMVMIASSSVRLRTTAERIASRATQLNIRNDPQIGSYAIRISSAAMHL
                     ASSAVRLASASARIR"
     gene            15926..17425
                     /locus_tag="VF547_00940"
     CDS             15926..17425
                     /locus_tag="VF547_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8261419.1"
                     /translation="MIRILSGPSIEAEDEQAVRSALSRWDGRSCLDVDPEIAFVAWDA
                     SRRLHLVQRDSLGSGTVYRRQKREAVSFSGTMPSLFEAGDLPRLSPRGVAVFLAMQFC
                     PAPTTVVETVTKLLPGEQRVEDAGGRLKHRRIVPAIRFGSAEPGGLDARECLDSIYRA
                     VARRASGRAVASLLSGGIDSTTNLVGLLRSGIEPAACTASFADPAFDERDAAAEVAGH
                     LGLPLRTRLVGVQEPGFLQHLAATVREPICDRALIPTALLLAGAEEAILVTGEGGDEL
                     WGPPRRWPAAASGGSAFPLQAYIEAISCSTAEVRARLLGDRALVRDADCGLSGDLERV
                     AEESLARTDYERAKAIQMFGWFAENVQVKDQAAAAASGLELVLPLVHRSARAMMESLA
                     ASRHYELTRSKALLRGYLSDQVPQAVLQRPKRKFKVPAALWRTDGLMRGIEEACSSQG
                     GVLGGILDYPALVEAARQADFDERILWGLFVLRFWFDRLLREVSARPST"
     gene            complement(17370..18662)
                     /locus_tag="VF547_00945"
     CDS             complement(17370..18662)
                     /locus_tag="VF547_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261420.1"
                     /translation="MERASAELVVGRESRAAFRFIFASALMSAISFSIVIPILPNLVR
                     EIAGGDTASAAEWMMVFASAWGLAQFLCAPLLGALSDRFGRRPVLLISTFGLGVDFLF
                     MALAPTLPLLLVGRMVSGATAASFATAHAYVADIVAKEDRAAFFGRLASSISLGYLIG
                     PVLGGWLGEIDLRLPYAAAASVTLANWLYGFFVLPESLAPERRSGRLELRSPIKAGGL
                     ALLRSRPLLARLAGISFLNSLANMLWGSVWVLFCAHRFGWSPLEMGIAILASGVAGVG
                     VQAWGVGRVVKAAGERNTLLIGAGVACATLAFTAFAPNGWWYAAIIPVAAFGLLLGPG
                     LQGMVSASVGPEEQGRAQGGLQGLNGIATIVGPPIYGMVFAWSLRQPGGTDLSGLALL
                     LSAAFMAGAFVLALGLGRQAPRSTAARTPPARADRTKI"
     gene            complement(18665..20689)
                     /locus_tag="VF547_00950"
     CDS             complement(18665..20689)
                     /locus_tag="VF547_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8261421.1"
                     /translation="MNQNEPARLEGPRTLVRAAALALMLAAAGTAARAAASAAQPPAA
                     GPTPQAVPVPPTAAGRASENVVTQAEDAFGTSIGRETIGLYSAGNVRGFSAISAGNTR
                     IDTLYFDQVQGPNPRIRRSTNIRVGLSALGFPFPAPTGIVDYALKSPGREPAASAYLY
                     QDSWGGAALELDAVLPLGGRLSLGAGAGLYKTEFGDGTDTEQHVEAATLRWTPSPGVD
                     VQPFWQRSQVYDDVGPIFVPAGPYLPPKMERRRFMGPKWAEHEGVSLLYGAKGVVAAG
                     PRTLVRIGLFRSALDDRIGAANLYNAVTRDGQAQHVVLIDPPGTFASNSGELRATHRR
                     QEGGRVHLLHASLRGRDRRRTYGGSDRFDLGTVRIGAEAPAPQPAFEFGEQSRDRVRQ
                     WTAGLAYEGRWERVGELSLGLQKTDYRKRVRLPGAAEATTGSRPWLFNATVALHASAG
                     LAFYAGYVRGLEESGVAPANAANRNEPLPAIRTSQRDAGLRWAIRPDLRFVAGLFEVR
                     KPYFQLDETSRFELLGDVRSRGLELSLAGKLTRELVVVAGAVLLDPRVTGEGVRLGRV
                     GKRPVGLAERTLRLNLDWRPAFARRLSLDLGIAYNSNRPSTRDNRVFVPQRALLDLGA
                     RYRLRQGARPLTLRLSATNLLDAYALDVLSSGTYYTIPGRLVALSLASDF"
BASE COUNT         3346 a         7173 c         7028 g         3302 t
ORIGIN      
        1 tccgtcgaga aggcgagcgc ggtcaccacg tcgggcaggt cggcgagaag cccggcgggc
       61 ttgagcgtcg cgcggtcgag cagccggacg aacggcgcgc cctcggcggc gccggcccgg
      121 ccgccgagcg ccacgtagcg aggcgacacg gccgcggcat agagcgcgcc ctcgtcgcgc
      181 ggcccgaccc ggccccgggc cacggcgagc agccgtccct tgtggttcca gctgcgcgcc
      241 gtctgatcgt cggagacggt gagcagccgg ccttcgtcga gaacgacgag gcggttgatg
      301 aaggcctggt gcgcgccggt ctcgacccgg atgatgggcg ccggctcgcc ggctgcggcg
      361 gccgaggagg cggcgaacgc ggccagcgcg gcgcaaaagc tgccgagcga gagcctcgga
      421 tcaaagcgaa aaggtggcat tggacgtatt cgctctggcg acgcgcagct ccgcatccag
      481 ggcgcctcgc ttctccatgc cttcgaacat cggctgagcg acggtgcgca cctcggggct
      541 gacccggctc aggttcactc gggccggccc ggtcgacgcg gaggcggtca ggaagcgcgc
      601 cgcgacgaaa cccttggatc cgtccgaccg cctgatctca tgccatccgc ccggctgcgc
      661 cgaaaggagc tccacggcct cccctcgcgc aagcttggaa accgcggcgg ccttgaccga
      721 cgcggcggac ctcacggtga gagcggtctt ggcggtaagg atcgccggtg gcggcgccgc
      781 cgcggccttg gccggcgcga attgctccag atagagttga tcatccggcc tgccttcggc
      841 gaggaagccg gcggcgctgc ggaactggac gagcgcgtcg tcatagctct tggcgaactt
      901 ttcggcgagc ttgatgtcgc gttcgagccg cttcttgtgc tcggcgagct gggatgtcgc
      961 ggcttcgcgg ctgagctcgc cccgaccgac cgcggccttg attcgaagcg cttccgtgac
     1021 ccggcagttc ctcgcctgac tgaaggcgcg aaggccccgg gccgcttcgg cattggcgtt
     1081 ggccacgtcc tcgtagacgg acccggcaag cgctcggcgg tcgctgttcg cccgcttctt
     1141 ggattcgaga taggcgcttc cggcgccggc ggcgactccg acgagcgcgc ccttcaaagc
     1201 gccttccgtc gagcccttgg tgagcagcag gcccgcgcct gcgcccaaga tgcctccgat
     1261 aagagagcgt tcgacaaaca gccgagaaac cgagccttca gccgtcttga ggtccttaga
     1321 ggcagcagcg cagggatcag taaagctgct tttcttcgct tctagcgcgg gaccatggaa
     1381 tcctgccact agagcgagag tcgtcagcca gaatctaaca gctcgcacgc tcttggtccc
     1441 ccgaacagaa agttgcttgc gaatcgtcgt tactggtgta ctcttgcaga tgcgctttgc
     1501 aatggtcttc tagactctgc acctcggcgc tggtggggcg ttacgacaat ataactttga
     1561 tcttctcggt acttagcggt tccgagatcg gctgtttttg ccctaagctg ggatggagac
     1621 tctaatgcac aaactaaatc tcgcgctgat cgccggactt ggcttgatcg cgagctcgcc
     1681 cgcgctcgcg caggacgacc cggacaccac catcaatggc gacacgacca tcaatgccag
     1741 cctcaagaat tcggccacgg gggccggcgg cgaggatgcc atcgcgacga ccgacgtggg
     1801 cacgatcagc ggttccaaga tcaacggcac caccgagatc aacgcgacgg cggaaaacgt
     1861 cgtcaccggc gcgctcggcg aaggggccga agcgaggctc gaggttggcg tgatcaacaa
     1921 cgccagcatc gacggcaata cccggatcaa cgcctcggcg gagaatatcg gaaccgtcgc
     1981 gatcggcgag aatgcctgcg cggccacccg ggtcggagcg atcggaacca agccgtgcaa
     2041 gaagcccagg tgacgcggag gcgcctcgcc gcggccgccg cggcgctcgt cgcgggcgcc
     2101 gcgctggccc aggtggtgac ggcgcagcag ctcaacccgc gcaagagcgc gccgccggcc
     2161 gcggaatcga agaagagccg aatggtcgcc cgtgccgagc tccaggcgca gcaggacggc
     2221 ggctcgctca agagcgagga gctgacggtg acgaaaagct gcgggaacgt tcagatcggc
     2281 ggtgcgccgc cgcccgatcc cggcaagtcg cggacgctcg tgggttccca gcccgggctc
     2341 cgggatcggg acaatgcgac ggtcgtctcg aacgtcgtga acatctgccg ctgaggcagg
     2401 gaggtccgcg gagatgatcg gaatgccgtg cctggcttga tgaggatggg gccgcggagc
     2461 ggcgtcctgc gggtggccgc tgcggccgcc ctgctgtcgc acgccgcggg cggagccggc
     2521 tgggccgcgc cgaagccggg caacgatccg cgccgcgcgg cggtggcggc cggccccgac
     2581 atgcgggcga cccggacggt cacgaatttc gcggaagcgc tgtcctgcat ggacgacctg
     2641 ctcctacaat atggcaagcg cgacatcgcc atcatcaacg acgggatccc ggacgcgacg
     2701 gcaacgctgc gggtcggcac ccgcgacatg gtcatctccg cgctggacgc catgtcgctg
     2761 aagagcggcg ccttcaaata tgtcgacgcc gatcagtcgg acgagaacgt ccgggtgatg
     2821 cagctcaacg tcaacggcag tctccagcgc gccgattact acatcaaggg ctcgatcagc
     2881 cagatcgacc aggaggtcct gtcgagcggg aagcgcatgg gcatcgcgac cgactttctc
     2941 tcggtcggcg cttcgaagga ccggtcggtc accaacatcg cgctggagct gggaatgtac
     3001 cggaccagcg accggaccct catccgcgga gtgcggactc agaacacgat ccaggtcgtg
     3061 cgcaagggca agggcatgga cgtgggcgga ttgctgccct tcgcctcgct cgtctacgag
     3121 gtccatagcg accgggcgca gggctcgcac cagacggtgc gcacgctggt cgagctcagc
     3181 ctgatcgaat tggtcggcaa gttcaccaag gtgccctatt ggcgctgcct ggcgctgccg
     3241 agcgccgacc cgatggcccg ccatcaggcc gacgattatt atgcgcgcat gcgtccgccg
     3301 gagcagatca tggcggccca gacggcgctg caccatctcg gctattatca ggggcagttc
     3361 gacggcgtgc agtcgccgca gctcggccag gcggtcagcc gctatcgggc cgagcacggg
     3421 ctcggatccg gcccgcatgt cgatttcgag ctctatttca gcttcctgac caaggggctg
     3481 gtggcgaacg gagacgccgg cgcgaagcgg ccgcggccga tcatggcgga gcccatcgcc
     3541 gacgacggtc ttcgcttcac gatgaagctg agcgacctgg tccagcaggg aagcgagctc
     3601 aagctggagc tgaccccgac caggaacgcc tatttctatt gcttcatggc ggggaacggg
     3661 aagcaggcct accgtatcta tcccaaccgc ttttccggcg accagccgat gacccaggcg
     3721 ggctcgacac tgcaaatccc cgctgagggc gacaagctgg ccatccggct caacgacgtc
     3781 gggatcgaaa gggtcgcctg catcgcccgc acccagcgct acgacgttcc gccgccttcg
     3841 ctcgccgcca acgatctttc gcccatcgtc gccagcggca agctggccgg gctcgattcc
     3901 gtgatcaacg agcaccagaa gcgcgattcg atgggcctga attcttccgt ccaggtcaag
     3961 gagatccagg tgacccgggc gagcgccagc cgatgatccg cctcgccgcg atcggcctcg
     4021 ccctcgtggc gccggcgagt cgcggcagcg cgcaggacga gcccgatacg accgtcccct
     4081 ctgctcagga ggcggccgcg caccgggtcg ccgatccggc agcgccggcg gcgaagcccg
     4141 cgcgcgcagc gacccgcgtc ggcgtctcga gcggcggctc cacccgtgtc gacgcccatg
     4201 cgcgcaacgt catgaccgtc gccgacgacg gcgtcgcggt gaccaacatc ggcgtcggac
     4261 ggccgggagc ctcgaccagc gtctatgccg aggacatcgt caccgtgggc cgggggcaaa
     4321 gcgccgagac ggacataggc accgggtccg gtttcgtcag cgccaccgac atctacaatg
     4381 ggggcgggcg cctcagcatc ggcgccgaag gaacgaagag ggacggcaag acctgcgtcg
     4441 agatctaccg caacacctgc atcgtccatt tctactaccg gcggaagaac gatccctgcg
     4501 cccccggcta ttggatcgac gggcgcaagt gccgcctgcc ttcggacagg aagcatcgca
     4561 taggcagctg agcccggacg ttcgcccagg ccgggctccc cactcggtgc cggcccgacc
     4621 gattcgcttt cggccgtcct cggcggtgtc gccgcccgcg atccgattgc ttgctcactg
     4681 atccgggacg gcgagtcgcg gcgagtcgaa tcgaaacatc ggtccggaaa atcggcttca
     4741 gccttagtcc aacgacgctc cgcgacactc gccgtctcaa gtgccaagtg ttctgaacgc
     4801 ggcgagtcgc cggtgtttca tgcctttgag ttgcgccgac ccaattggaa gcggaggcga
     4861 acgccgaaat tgcattttcg ctcaatggcc tgattgcaac gctcttgcgt atccgatatg
     4921 agtttaaacg tctggcgcgc tttcgcgtca gcttgaaaga gaggtgtttg gtggctgata
     4981 tcaacctgga aacccaagag cacgatctcg acgacgattt cgtcgttctc gacgctgatg
     5041 atttcgttga agtcgctggt ggcaccggcg gccatacgca agtcggctcg gcgttcaaag
     5101 cttgaatcga tggttctggc gcgatgggct cggttcgtcg cgccagtcca tcggcagcaa
     5161 aaaaggctgc ccgtaaagac ggctccgagc ggagccggcg gagggcgatt cgccgccttc
     5221 ccttgcaatc gtccctcgac ctgaagcgcc cctcgtgacc ttccgcctca gatgtggcag
     5281 gctcttcgcc gacgccccgg acatcgtccc tgcggatcaa tccctgggcg gaaccatgcc
     5341 gctccgcgcg cgccagcatt gccttccctt ttccctcgcc tcgggatggg ggtaccacgt
     5401 tcactcgccg atcaacttca aggcgatgtg ggacggcaag cagatcttct ggaattacgg
     5461 cgatgtcaaa ggctggcttc cgctcaacac cgcctatttg cccaattcct ggcagcgcca
     5521 ctccgagcac gctcccgccg ggtccgagga gttctgcccg atcttcctgg aggccttccc
     5581 ggagccgggg gtcatgcaga tatggtccgg ctacgtcgcg cagacgccgc aggactggag
     5641 cctgctggtc cgctccccga tcaaccttcc gggctccagc ttctacgagg tgatcgaagg
     5701 gatcatcgag accgactggt gggtcggccc gctcgtcacc ttcatccgct tcaccaagac
     5761 ggacgtgccg gtgaagttcc gccgggccgt cccgctcttc caggtggctc cgctcccgcg
     5821 cggcgcctat tcgttccaac agccggccga gctcgttcac gaggagccgt tctcggaatg
     5881 gtcggaggcc gattgggacg ccttccggat caccttcgag aagcgcaaca gcggcaagcc
     5941 cggcgattat gcccgctacg cccgcaagcg gcccccgccc gaggccctgt gacggtcgat
     6001 ccggtcgccg agaacgctgg cgccggcaaa ccccgcctgt tccgggcggc ggcgatgaac
     6061 gagttcgagc gccgcggcgc gggcgagccg atccttcagc agccgcccaa tttcaccacc
     6121 tatctgatcg ccatctcgat cctgtgcgcc gccctgttcg ccttcgtcgc gctcgccacc
     6181 tggccggcgg tccacacggc ttcgggctac gtcatcccct cgcgcagccc gatcgagatc
     6241 tacccgtccg gatcgggggt ggtgatcgcc atcgccgcgc cgggcagccg ggtccgggcg
     6301 ggccagccgc tggcccgcct gtccgccgac acctcctcgc tcacgggttc cggcaccgcc
     6361 gaagcgcgcc tgcaggcgct cgagagccag cgccaggcca acagcaacga gatccgcctc
     6421 gtcgacatgg atcgcaagag ccggcttgcg cagctcgatt ccgagcaggc cctgctcgcc
     6481 gccaggatcg aaaacctgcg caggcagtcg gccatcgcca atgcgcgcat cgccaatgcc
     6541 gagcaggacg tggcggcgag ccggacgctg gtcgacaagg gctatatggc ggctcacgag
     6601 atgcggcggc ggcaaaacct gctcctcgag gcgaggcagg ccatcaccgt cctcgacctg
     6661 gaagcggtcg cggcccgcaa ccggctgcgc gcgatcgcct tcgagaagga ttccatctcg
     6721 gcctccttca gccggcaggc cgaatcgctg agggccgaaa gcttcgcccg gagcggcgaa
     6781 atcgcgacgg ctcggctggc cgtcggcgag accgtcgtcg ctccggtttc ggggcgggtg
     6841 gcgtcgaacg aggttcaagt cggccagcgc gtggacaaac gcgcccgcct cgccatcctt
     6901 cttcccgaag cgacccgcct gcagatcgag ctggcgctgc cctcgcgggc cgcgggcagg
     6961 atcgctccgg gccagcgggt cagcctcgac ttcgactcct atccggcggc caagtacgga
     7021 cggcagaccg ccgtcgtctc ctcggtggag ccgatcgcga cgcgctcgtc cgccggggcc
     7081 atcgccgggg ctcggaccga gccggaattc cgcatcctgg tccgccccga ccgcgacgtc
     7141 atcgcctatg acggccagac atatccgctt cggcccggaa tgaaggttcg agcgcggatc
     7201 gtcatcgagc gccggaccct gctcgcctgg ctgctgagcc ccttcaagac gttcggatga
     7261 cgatgggcat gaccgaccag tcgccgcttc tcttcagccg ccggcgcaag gtgccggtca
     7321 tctatcagct cgaggccgca gaatgcgggc tcgcctgtct cgcgatggtg tgcggctatt
     7381 acggcaagca tgtcggcatc ttcgagctga ggcattcatt ccggatctcg accaagggcg
     7441 ccacggtcca gagcctggtc caggccgcgc gatcggccgg actgctgtcg cgggtgctca
     7501 aggtcggcat ggggtccatc caccgtcttc agctgcctgc ggtcctccac tggaacctca
     7561 accacttcgt ggtgctcgcc gagcgcaagg gcggcaaata tctgatcaac gatcccgcca
     7621 ggggcgctca ctggctcgac gaagaggagg tgtcgaagca gttcaccggc atcgccatgg
     7681 agctcacgcc ccaggagggc ttcgagaccc ggaagcgggt cccggccctg accttctcga
     7741 cccttttgag aggagtggtg ggagcgcgcg gcttcttcgt gcagctggcg ctgctttcgc
     7801 tcgtgcttca gctcctcgcg ctggtcagcc cgctcgccgt ccggctcatc atcgacaacg
     7861 ccgccatgca aggcgacagc gagttcctcc tcgtcctcgc cgccgggctg gggatcgtca
     7921 tcctcgcttc gatcgcactg accgtgctca ggtcgaagct catcgtctat ttctcgaacc
     7981 agttcagcta tcatgtggcg gtccgagtct tcgcgcacat ggtggctctg ccggtcgcct
     8041 atttccaggc gaggagctac ggcgacatcg tcatgcggta tcgcgcggtc gagccgatcc
     8101 gggacctcat caccaaggcg ttcatccccg ccgtgctcga cgggatcatg gggatattga
     8161 cgcttctcgt cctgttcctg ctgtccccgc tgctggcgct gatcccggcg gcggcggcgg
     8221 ccgggctggc ggcggtctat ctcgccagcg ccgcgtcgag ccggtcgttc caggaagccc
     8281 ggaccacgac cagggcgatc gacatcagcc gggtgctgga gctcgtccgg agcatcgaga
     8341 cggtgaaggg cttcggcaag gaggacctca cccgcggcca ttggcagaac ctgcacgccc
     8401 agttcatcaa cgccaacatc aatgtcgacc ggctcgccgc ccggctggcg acggtcaaga
     8461 cctccgcgac cctggccgag gagggcctcg tcatcgtcat cggcgccgcg atgatcctcg
     8521 gcggaagcct gaccgtcggc accctgctcg ccttctacct gctgaaccgc ttcttcatcg
     8581 agaagatcag ccagttcacc gagcagttcc tcgccttccg cgacacctcc gtgcagctcg
     8641 accagcttgc cgacatcacc accgccgagc cggagcagac cgccagccgc tcggccgggc
     8701 tgcaggacgt caagggcgcc ctttccgtcg acgacgtctg gttccggtac ggcgatgccg
     8761 aggaatgggt gctcaaaggc gcgtccttcg aggtcgagcc gggcgagcac ctcgccatcg
     8821 ccggcgcctc gggcggcggc aagacgacct tgctgaaggt cgccagcggc ctgctgatgc
     8881 cgacgtccgg agcggttcgg ctcgacggca tcccgatcgc tcggatcgac gtcgcctcgc
     8941 ttcgcgaggc gctcctgtgc gtccagcaga acgaccatct gctcaccggg acgatcgcgg
     9001 agaatatctc cttcttctcg cccacgctcg attgggaccg gatcgtggcc tgcgcgaaga
     9061 tggcctacat atatgacgag atcatgcagt tcccgatgca gttccacacc ttcgtcggcg
     9121 atctccagaa cagcatatcc ggcggccaga agcagcggat ctgcctcgcc cgagccttct
     9181 accagacccc gaaggtgctc ggcatcgacg aaggcacgtc gcatctcgac cctcgggccg
     9241 aaagcgcggt cacgcgcacg atgagcggcc ttggaatcac tcgaatcacc gtcgcgcacc
     9301 gaatcgagac gctgcggacg gcggatcgaa tcctgctgat cgaggacggc aagctccgcc
     9361 ccgtcagcct ggatgagatt aaggagatag gaaatggtcg cgcaagccgg gatcgagcct
     9421 tcgccggcgg agggtgaact ggaggaggag cggcttcggc tccaggccat ccggtcggcg
     9481 gtggccctgg ccggcggcga gccgcggggc gggctggcgg cgatgcctcg gcccgccgag
     9541 ctgcgcgtcc tcgccgcctc gaggctcgac agcatcgcct atctgcagtt ccagcccgac
     9601 catccggtcc agcagctcta cgacgcgatc tggctcgctc agcaagccgt ggtcgagcgg
     9661 ttcgtcgagg ccgccgcggc ggccgggctg gtcgtcctcc ccgtccaggg catcgagaag
     9721 aacgcacgct acctgcgcgg ccattcgctc aaccgccgcg gagacctcga cttcatcgtc
     9781 cgagccgagg acctgccggc cctggcccgg atagcggaaa gccagggatt ccggcaggcc
     9841 gccatcgccc acgagggcgg gttccttccc ctgaccgccg agcagatggg catgggcctc
     9901 gccgcgagcg gaatggaata tcagcgcgaa tatccgttcc acggcttcat gccgttcgtc
     9961 gcgccggagc ggatgctggc ccgcataccg caggcgctca atccgcttcg gcgggtcgga
    10021 tcgaaggtcg agctcatcct cgcgctcgac atgatgaccg cctaccacga gagcgccccg
    10081 gcgccgctcc actgggagag gctgcgcgcc agcggctttc gcggcgccca gacccaggcc
    10141 gccgagctcg acctcctgtt ctccgcccat cgcctgtacc agaagctcgc cgtggcgccg
    10201 gggccgcatg tcacccggat gctggccgag ctcgtcctcg tcctggtcgc cgagcccgat
    10261 ctggactggg agttcctcga gtcggcggcg atgacctgcc ggatgggcgc ggcggcctat
    10321 tacggcctcg gaacggctgc cgccctgatc ggccatccgt cgctgcattt catcgagacg
    10381 aggctcgcga accatgcgga cggctcgatc cgcgacgaag gctggctgcc ggccaggctc
    10441 ttccccgccg gcctgctcgc ggccgaaatg ccggactggc tcatccggtg agaccgacga
    10501 ggcccccggc tgctccacgg gttggaacgg tctgatgtgc gggattgccg ccatcttgtc
    10561 ggaagacctg ggcgaggctg ccgtcgaagc caggaccctg gccatccggc ggatggtcgc
    10621 cgctcagacg tcccgcggac ccgatgccga gggggtctac tcgcacggcc cggtgaccat
    10681 ggggcacaac ctcctgcgaa tactcccgtg cccgaactcg acgcagccgt tcacgtcgtc
    10741 ggacggcgcc ctgaccatct gctacaatgg cgagatctac aattatctgg acctgcgggc
    10801 cgatctgatc cggcagggcg tccggttccg ttccgacacg gacacggaga cgatcctcga
    10861 gctctaccgg cgggacggac tcgacgcctt cgcgaagctg cgcggcatgt tcgccctggt
    10921 cctgcacgac gtccgcaacg gccgggtgat cgccgcccgg gacgccatgg gacagaaggc
    10981 cttgtacatg cggccgtcgg ccgacggcct gtacctggcc tcgaccgtcg ccgcgcttcg
    11041 ggcgggctgc agcgaacccg ccgagatcaa tcggcgcgcc ctgcatttct acctgtggac
    11101 gctgatgcct ccggcggagg agacgatgct caccggcatc gagctgcttc cgcgcggaca
    11161 ggccgcgata ttcgacgccg gcggccggct gcttcgaatg acctcgttcg cgggcgccga
    11221 cggcgccggc gccgtggagg acgacctgga ccgcgccgga gcgaccctga ccgcgctgct
    11281 ggccgccgcg gtcggcgagc agtccgaagg ggtgccgagg ctggcgaccc atctcagcgg
    11341 cggcctggac tcgagcgcga tcctctcggt tctcccggcc tcgcttcgcc cggacgtgac
    11401 gtcatacacc tgttcctaca aggtgaactc cgtcgacgtg ccggaggagg aacgcgggtt
    11461 cgaggaggcc ttcgtcgcga ggagggtcgc ggactatgtg ggcgtccgca acgaggtcgt
    11521 cctcatcggc gcggaggaat atgcggccaa tctgttcgac gtcgtcgcga cggtcgacga
    11581 accccgcgga aatccctgcc tgccccattt cttcctcgcg cgccgggtcg cggaacggca
    11641 ccgggtggtc ctcagcggcg agggggccga cgagcttttc gggggctatc cctggaagac
    11701 tcgttgcgcg gagtcgccgg acgggtcggc ctttctgcag gcgatggcgc cggccccggt
    11761 cgagctgctc gagcggtcgc tcgacggccg ctgggccgac caggcggacg tgcgcgacta
    11821 tgtcctgtcc tccgtcgcgg cgccgacccg cgacgagcgg ctccggaaga tattggagtt
    11881 cgaccaggcg cattttctcc agtatctgct cctccaggcg gacaagctgg gcggtcgctt
    11941 cagcatagag gaccgctatc ccttcctcga ccgcaggatc gtcgatttcg cccgaagcct
    12001 ctcgccgcgg ctgctcagcg gcggaatggc gaactccaag cccgttctcc ggcgggcttt
    12061 gaagaatttg gtgcccgatg aagttctcac ccggcccaag atgggcttcg tcgcgcctga
    12121 aggaagctgg taccgccatt ttctctcgcc gttattgcgc gatatcctcc tgtcgccgga
    12181 ttccttcgtc cggaccttct acaaaacggg ggcggtcgag catattctgg aggatcattt
    12241 ccggggccgt gcgaattatc gcaagcttat ctggtcgctc ttgacgcttg agatttggca
    12301 ccgcaccgtc attcgcgcag aaggagcggt cgacttgcag caggagtatc gcaatggccg
    12361 ggccgcgtga cagggccatg cgcgaatcgc cggtccggcc tggagccggg agggcatgat
    12421 cgacgacagc tggctgcttc tgtggagcgg atccgcgacc gtcgattcgg tggatcaact
    12481 tttctccccc cgcgactggg ccgcgggcat cgattcgggc gagctccggc gcgtcgagca
    12541 ggactgggcc gcggcgctcc gcaaggcgca ggagcagggt cgacgcctgt tcgactctcc
    12601 ggccgtccgc ctctgcggcc ttgaagtgcg ctcgggccgg ctgcagcttc gatacgagct
    12661 cacccgctac gcctattatg tcggcgcccg ctcgcgatcc gtcccgacgc ctttttcggg
    12721 caatccggac ccgaccggga cggtggtgat cggagtgacc gccgactcaa agattccgat
    12781 cggcaagcgc caccaggacg tcgacgccaa tcccggcaag ttcttctgct tcggcggctt
    12841 cctccatccg acggaagacg cctccgccga gggaattccc gatgccttcg cctgcgcccg
    12901 gcgggagtgg cgggaggaaa ccggctcgga cctcgaaggg gcgggcctcg agctgatcag
    12961 catcgtctac gacagggtct atcctcatcc ggagatggcg ttcctcgccc gcaccgccgc
    13021 gacctccggc gagttcgtcg cctctcggtg ggatgccgag ctttccgacc tctccttcgt
    13081 gccggtcgac gggatcgagg aattcgtccg gaggcaccgg accgatttca ccgaaagtct
    13141 ggtcggcgcc ttcgaggtcc tggcgaagcg ctaccggatc gggcgagatg ctgactgagg
    13201 acggcttctt cggcggcagc gagttcggcc gggcgttcga ggcggatctt cgcgccgccc
    13261 tcgcccccga acagctggcg atgctgagcc agctctgctt cgtcctgatc aagcccgacg
    13321 ccctgctttc ggggaaggcc ggcgaaatcc tcgatcggct tcgggcgagc ggcttcgaga
    13381 tcgtcttcgc ccacctgctc caggccccgc gggaaagcca gttcgaggac ctctacaaat
    13441 acaatctcac gctttacaac gtgaagcatc agctcggcgc ctggtggctc aaccggcaaa
    13501 tctacggact cggcccgtcg ctctcgctcc tcgtccgcgg cggcgatggg acggccggcg
    13561 acatcgcggc ccggctcggc cggctcaagg gcccgtcgtc gccctacaac ggagcgcccg
    13621 ggcagatccg atgggacttg caggcgacca accgcagcct caatctcgtc cattcggccg
    13681 acgggccgct ttcgaccgca cgggaattcc agctcttcca cccgccggag gcgctgaagg
    13741 cggtcctcga ttcgatgatc gagggagcgc ttcataccgc ccactcgaaa gccggccgga
    13801 tggtcctcgc atttcgcgcg tcgcgtcagg aattctcctt ccttccgacg ctgctcgccc
    13861 tcaaggcgcg agtggcgatc gccggctatg gggattcgaa cgaagccgcg acggcgcgcc
    13921 tgctcgatct ttattcgtcc atcggcgccg cctcatccat gacggaggag gtgcgatcga
    13981 tccttcggct cgccgaacgc gagcgggagg tcgcgggcgc gctttggccg gccgacggcc
    14041 cgctcgacca gctgttccgc ctgctggcag cgccggagcg atacggcccg gcccttgccg
    14101 ccgcgctcag ccatcgcctg aaggcttgcc agatccacgt gtccggcatc gagctgctcg
    14161 tgctccagac gtcgatatac tattttgcgg ccgacgccct ggcggcggaa gcgcactcgc
    14221 ccgcctgacc tcaggcgagc ctttgcgttt cgaggctgtc gagaagcgcc ggcagcaaag
    14281 ccccccggtt cacccgctcg ttgcgcgcgg ccgtcgcttc ggcaaggcaa cgccgggcca
    14341 cggattcggg aacgagcgcg ggatcgagct ggcggagcac ttcgaaaacg tagtggaacg
    14401 cgacgaacag gccgtacaat tcgccgactt cgatcaccca gtcccagtcg atcgccggcg
    14461 cggcgtcgag gatggcggcc aggtcgacca ggctgcgcag gaccggctgc ttctggtcgg
    14521 tcgcctcgac gtagaggcgg gccgccagga accagatttg agtctcccag gcgagcccgc
    14581 ggcgggtcct gccccaggcc tggacctccc ggcccccgcg ccagaccacg tcgagggaga
    14641 gatcgaacga cagattataa tggacgtcga tctcgatgag cagccagact tcctcgccgc
    14701 aggtcaggat ataatccctg ggcacgcaat aagagttgat gatgcttcgc tgcgcatcga
    14761 gctcggggca ccggacgagc ttcaggaagg ggggcatctc gtaatgctcc ttcaggaagt
    14821 tctcgaactc cgtcgaggtc agctctgtca ggaccagtgt gtcgcgattg aacgctccct
    14881 ggcggaagcc cagctccgtg aatgcctcga tggtcgacgc gaggaactgc ggtttgacga
    14941 gaaggtccac gtccgccatc tggcgcggcc gcgagcgcgg atagagcgac cagacgtaat
    15001 ccgacccttt gatcagggcg ggcgcgattc cgcgcgcttc gagagcgtcg agcggcgctc
    15061 cggcctcttc gaacaggcgc cgctgggcgg cggcatggac tccgctcagg gcttggagag
    15121 gctcccgccc cgagagcccc atcctctccg cggcatgatc gagcgcggga accagccggg
    15181 tctgctcggc ccgctgcaac aggtagggcg aaacggagga cggtagcgga tcgattcctt
    15241 cgatcaggcg ctcggctgcc gcgcagaggc gctcgcgccc gctgcccagt agccccgagg
    15301 ccagaagcag cagattctca ttccggcgtt cgtcggcgtc gctgagggct tcaggcaatg
    15361 atcttccttc cgcatcgacc tggctgcccc gagggcgacc ccgccgtcag cggatgcggg
    15421 cgcttgccga tgccaggcga accgccgagg atgccagatg catcgccgcc gagctgatcc
    15481 ggatagcgta ggagccgatc tggggatcgt tcctgatgtt gagctgggtc gcgcgcgagg
    15541 cgatccgttc cgccgtcgtc cgaagccgca cgctgctgga agcgatcatc accatggccg
    15601 aggcgaccca ctgcgcgtgc gcagccaaat catccggctt cgaaacgctc ctgagtcggg
    15661 gtccgagctc agccgaggca gagccgatcc gtgcagcggc acttgccagg taatccagcg
    15721 gcgtctgggc ttccttaacc gcgggagcat tcttctccat cggaacctcc aagcacaata
    15781 gactaatcta tactgcctca aaatctgtgt ggagtctaga aaggcgatag gaaagcggcc
    15841 taattctgct cagggctgca atcgagtcta attcgagcgc gacaactttg acccgccacc
    15901 caagcggcgg caagactgcg ccgcgatgat tcgaatcctc tccggcccgt ccatcgaagc
    15961 ggaagacgag caggcggtgc gttcggcgct ttcgcggtgg gacggccgct cctgcctgga
    16021 cgtcgatccg gaaatcgcct tcgtcgcgtg ggacgcctcg cggcgactgc acctggttca
    16081 gcgggacagc ctggggtcgg gcacggtgta ccggcgccag aagcgcgagg ccgtgtcctt
    16141 ctcagggacc atgccgtccc tgttcgaagc cggcgacctt ccgcggcttt cgccccgcgg
    16201 agtcgccgtc ttcctggcaa tgcagttctg ccccgccccc accaccgtcg tcgagacggt
    16261 gacgaagctt ctgccgggcg agcagagggt ggaagacgcc ggcggtcgtc tcaagcaccg
    16321 tcggatcgtg cccgcgatcc ggttcgggag cgccgagccc ggcggcctgg acgcccgcga
    16381 atgtctcgac tcgatctacc gggccgtcgc ccggcgggcg tccgggcgcg ccgtcgccag
    16441 tcttctgagc ggaggaatcg actcgaccac caacctcgtc ggcctgctca ggtccgggat
    16501 cgagcccgct gcctgcaccg caagcttcgc cgacccggcg ttcgacgaac gggacgccgc
    16561 ggccgaggtc gccggccatc tcggacttcc gcttcggacc aggctcgtcg gcgtccagga
    16621 gccgggattc ctccagcatc tggcggccac ggtgagggag ccgatctgcg accgcgccct
    16681 catcccgacg gcgctccttc tggcgggagc ggaggaggcc atcctggtca cgggcgaggg
    16741 cggagacgag ctctggggcc cgccgcgccg ctggccggcg gcggcgagcg gcggttcggc
    16801 ctttccgctg caagcctata tcgaggcgat aagctgctcc accgccgagg tccgggcgcg
    16861 gctgttgggc gaccgggcgc tggtccggga tgcggactgc ggcctgagcg gcgatctcga
    16921 gcgcgtcgcg gaggagagcc tggcgcgcac ggactacgaa cgggcgaagg cgatccagat
    16981 gttcggctgg tttgccgaga acgtccaggt gaaggaccag gcggccgccg cggcgtcggg
    17041 cctggagctg gttctgccgc tcgttcacag gagcgcccgg gcgatgatgg aatcgttggc
    17101 ggcgagccgg cattacgagc tgacgcgttc gaaggcgctg ctgcgcggct acctttcgga
    17161 ccaggtgccg caggcggtcc tgcagcggcc gaaacgcaag ttcaaggtgc ctgcggcgct
    17221 gtggcggacg gacggcctca tgcgcggcat cgaggaggcc tgctcttcgc aaggcggagt
    17281 cctcggcgga atcctcgatt atccggctct ggtcgaggcc gcccgacagg ccgatttcga
    17341 cgaaaggata ttgtggggcc tgttcgtcct cagattttgg ttcgatcggc tcttgcggga
    17401 ggtgtccgcg cggccgtcga cctaggagct tggcggccga ggccgagggc gaggacgaag
    17461 gcgccggcca tgaaggcggc cgacagcagc agcgcgaggc cggagaggtc ggtcccgccc
    17521 ggctgccgaa gcgaccaggc gaagaccatc ccgtagatcg gcgggccgac gatggtcgcg
    17581 atcccgttga gcccctggag gccgccctgg gcgcggccct gctcctcggg accgacgctg
    17641 gcgctgacca tcccctgcag gccggggccg agcagcagtc cgaaggccgc gaccgggatg
    17701 atcgcggcat accaccagcc gttgggcgcg aaggcggtga aggcgagcgt tgcgcaggcc
    17761 acgccggctc cgatcagcag ggtgttgcgc tccccggcgg ccttgaccac ccgcccgacc
    17821 ccccaggcct ggactccgac cccggcgact ccggaggcga ggatcgcgat tcccatctcg
    17881 agcggcgacc agccgaagcg atgcgcgcag aacagcaccc agacgctgcc ccagagcatg
    17941 ttggcgaggc tgttgaggaa gctgatcccc gccagccgcg ccagcagcgg cctcgaccgc
    18001 agcagcgcca gcccgcccgc cttgatcggc gagcgcagct cgagccgccc ggaccggcgc
    18061 tcgggcgcca gcgattcggg gaggacgaag aagccgtaca gccagttggc cagggtcacc
    18121 gaggcggcgg ccgcataggg cagcctcagg tcgatctcgc cgagccatcc gcccagcacc
    18181 gggccgatca gatagcccag gctgatcgac gaggcgagcc ggccgaagaa ggcggcgcgg
    18241 tcctccttgg cgacgatgtc ggcgacatag gcgtgggcgg tggcgaagct cgccgcggtc
    18301 gccccgctca ccatccgccc gacgagcagc aagggcaagg tcggggcgag cgccatgaac
    18361 aggaagtcga cgccgaggcc gaaggtcgag atcagcagca ccggccgccg cccgaagcgg
    18421 tccgacagcg cccccagcag cggcgcgcaa aggaactggg ccagccccca ggcggaggcg
    18481 aagaccatca tccactcggc cgcgctcgcc gtgtcgccgc cggcgatctc gcgcacgaga
    18541 ttgggcagga tcgggatgac gatgctgaac gagatcgcgc tcatcagcgc gctggcgaag
    18601 atgaagcgga aggccgcccg cgactcgcgg ccgacgacca gctcggcgga cgcccgctcc
    18661 atgatcagaa gtcgctcgcc aggctgagcg cgacgagccg gccggggatc gtgtagtagg
    18721 tccccgagga cagcacgtcg agcgcatagg cgtcgagcag gttggtcgcc gacagccgaa
    18781 gcgtcagcgg ccgcgccccc tgccggagcc ggtagcgggc gccgaggtcg agcagggccc
    18841 gctgcggcac gaagacccgg ttgtcgcggg tcgagggccg gttcgaattg taggcgatcc
    18901 cgaggtccag cgagagcctg cgggcgaagg ccggccgcca gtcgaggttc aggcgcaagg
    18961 tccgctcggc gagcccgacc ggccgcttgc cgacccgccc gagccggacg ccctcgccgg
    19021 tcacccgcgg gtcgagcagc acggcgcccg cgacgacgac gagctcgcgc gtcagcttgc
    19081 cggccagcga cagctcgagg ccgcggctgc ggacgtcgcc gagaagctcg aaccggctcg
    19141 tctcgtcgag ctggaaatag ggcttgcgga cctcgaacag cccggcgacg aagcgcaggt
    19201 ccggtcggat cgcccagcgc aggccggcgt cgcgctggct ggtccggatc gccggcagcg
    19261 gctcgttgcg gttggcggcg ttggccggag ccactccgct ctcctcgagc ccgcgaacat
    19321 agcccgcata gaaggccagc ccggcgctcg cgtgaagcgc caccgtcgcg ttgaacagcc
    19381 aggggcggct gccggtcgtc gcctcggccg ctccgggcag gcgcacccgc ttgcgatagt
    19441 cggtcttctg aagcccaagg ctgagctcgc cgacccgctc ccagcggccc tcgtaggcga
    19501 gcccggcggt ccactgccgg acccggtcgc gcgactgctc gccgaactcg aacgccggct
    19561 gcggcgccgg cgcttcggcg ccgatccgaa ccgtgccgag gtcgaagcgg tccgagccgc
    19621 cataggtccg gcggcggtcg cggccgcgca ggctggcgtg gagcagatgg actcgcccgc
    19681 cttcctggcg ccggtgggtc gctcggagct cgccgctgtt cgacgcgaag gttccgggag
    19741 gatcgatgag gacgacgtgc tgcgcctgcc cgtcgcgcgt gaccgcgttg tagaggttgg
    19801 cggcgccgat ccggtcgtcc agcgccgatc ggaacaggcc gatccgcacc agcgtgcgcg
    19861 gcccggcggc gacgacgccc ttggctccgt agagcagcga cacgccttca tgctccgccc
    19921 atttcgggcc catgaagcgc cgccgctcca tcttcggcgg cagataggga cccgccggaa
    19981 cgaagatcgg cccgacgtcg tcatagacct gcgagcgctg ccagaagggc tggacgtcga
    20041 cccccggcga aggcgtccag cgcagggtcg ccgcctcgac atgctgctcg gtgtcggttc
    20101 cgtcgccgaa ctcggtcttg tacagcccgg ctccggcgcc gaggctcagc cggccgccga
    20161 gcggcagcac cgcatcgagc tcgagcgcgg cgccgcccca gctgtcctgg tagagatagg
    20221 cggaggccgc gggctcccgg cccggcgatt tcagcgcata gtcgacgatc ccggtcggcg
    20281 ccgggaaggg gaagccgagc gccgacaggc cgacccggat gttggtcgag cggcggatgc
    20341 gcggattggg cccctgcacc tggtcgaaat agagggtgtc gatgcgggtg ttgccggccg
    20401 agatcgccga gaatccgcgc acgttcccgg ccgaatagag cccgatcgtc tcgcgcccga
    20461 tgctggtccc gaacgcgtcc tcggcctggg tgacgacatt ctcgcttgcc cggccggccg
    20521 ccgtcggcgg gaccgggacg gcctgcggag tcggtccggc cgcgggcggc tgggccgccg
    20581 aggccgcggc gcgcgcggcc gtccccgccg ccgccagcat cagcgccaag gcggcggcgc
    20641 gcacgagcgt cctcgggcct tcgaggcgcg caggttcgtt ctggttcatc ggacgggcct
    20701 tgtcgtgcgg gcgttcgcgc agtcggcgcg ctacccggcc gcctccagcg tcagcggcct
    20761 gcggtcggcg acctcggccg gcatggccca gctcgcgaag gtccgcccgg acttcaggtc
    20821 ccgcgccagc cgccgcgacg cgcgaagcg
//
LOCUS       DASVQZ010000035         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_105673, whole genome shotgun sequence.
ACCESSION   DASVQZ010000035 DASVQZ010000000
VERSION     DASVQZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105673"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..687
                     /locus_tag="VF547_00955"
     CDS             94..687
                     /locus_tag="VF547_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX8261422.1"
                     /translation="MRLLLASKSEARRRMLEAAGVRFETVEAELDEGAAKAGLWGAGF
                     DARGVAEELAQLKALSVEAGEDCLVLGSDQTLETADGTLLGKPASREEAWGQLSLLRG
                     ATHHLHSAAVVAEAGEAVWWNCETVSLSMRSFSDEFLHAYLDAEWEQIRWSVGGYRIE
                     GPGAQLFERVEGSQFAILGLPLLPLLGYLRERGLLKS"
     gene            687..1496
                     /locus_tag="VF547_00960"
     CDS             687..1496
                     /locus_tag="VF547_00960"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX8261423.1"
                     /translation="MGIPYAEVIGDPVAHSKSPLIHNFWLRRMNLVGDYRRTRVGAEA
                     LGDYLAERRGDPDWRGCNVTMPCKEAIAAHLDTVDPVARRIGAINTVVPRSAGLLMGT
                     NTDFQGAHFALCDCAPAGSKAVLLGTGGAARAVLEELRQAGAAHVTILSRAEEKARRL
                     LDSFGVDGAVGPIGGAPVADLLINATPLGMEGWPPLDLDLSGLRAGATVFDLVYHPIE
                     TPLLAEARRLRLRTIDGLAMLIEQAAMAFTYFFGEPPPDRYSPELRELLTR"
     gene            1493..2086
                     /gene="coaE"
                     /locus_tag="VF547_00965"
     CDS             1493..2086
                     /gene="coaE"
                     /locus_tag="VF547_00965"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369101.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX8261424.1"
                     /translation="MITLGLTGSIGMGKSTVARMFADEGVPVFDADAAVHRLQGRDGA
                     LVEAIEAHFPGTTGTEGVIRGALAERVLGEPEALRRLEALVHPAVAAERQAFLAANRG
                     SPLVVLDIPLLFEAGGADQVDRIAVVSAPAEVQRQRVLSRPGMTPEKFERILERQLPD
                     SEKRARADFVIPTGGSLEETRRAVRRILACLVPPADS"
     gene            2095..2808
                     /gene="dnaQ"
                     /locus_tag="VF547_00970"
     CDS             2095..2808
                     /gene="dnaQ"
                     /locus_tag="VF547_00970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX8261425.1"
                     /translation="MREIVFDTETTGLSPLAGDRIVEIGCVELVNRVETGRTFHAYFN
                     PDRPMPSGAEAVHGLTDVFLADKPRFHDRVEDLLEFLGDCPLVAHNAGFDFGFLNHEL
                     NNCGRPLICLTRMVDTLQLARSRHPGAKHSLDALCTRFGVDRSLRVKHGALIDAQLLA
                     QCYVELTGGRQIGLTLTDIVAEPIERDAPAGPDRSRDVLIEIRPPRAHAPTEEELQAH
                     ALFLTTLANPIWHRLEGRG"
     gene            2860..3237
                     /gene="raiA"
                     /locus_tag="VF547_00975"
     CDS             2860..3237
                     /gene="raiA"
                     /locus_tag="VF547_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX8261426.1"
                     /translation="MDIRVSGHQVDTGEALRERVGTRLTAVADKYSSRLVSAQVTFGK
                     GPHDHGFTCEILAYAPQGVVLKAADRAAAAPAAFDAAAEKIEKQLRRHSRRLKDHHQD
                     AAAELEAAEGYGYRRGEPEPQGE"
     gene            3309..3776
                     /locus_tag="VF547_00980"
     CDS             3309..3776
                     /locus_tag="VF547_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX8261427.1"
                     /translation="MNDMTDILTVDRVDAGMTVPNKKGLFQLLAVAAARRTGLDSKTI
                     AAALSEREKLGSTGFGGGVAIPHARLAGLNGVFGYFARLAAPVDFQSVDTLPVDLVFL
                     ILSPPDAGADHLKALAAVSRVLRDRNTVAKLRGARSRDALFALLSGVESRDAA"
     gene            3766..4227
                     /locus_tag="VF547_00985"
     CDS             3766..4227
                     /locus_tag="VF547_00985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8261428.1"
                     /translation="MPPDPEAGAAAHFRALESLYRHAPVNGLFESRLEIVEKGFARIR
                     FEVGEPLFHAAGAAHGTLYFKMMDDAAFYACNSLVTDRFLLTTAFNLLFTRPLRAGTA
                     TAEGRWASGRRRVLVGEARLLDSDGQLAASGTGTFMRSHIPLSGLPGYSAG"
     gene            4227..>4526
                     /locus_tag="VF547_00990"
     CDS             4227..>4526
                     /locus_tag="VF547_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEX8261429.1"
                     /translation="MEPRIAASVLAGALIRAAEGEGGFGAVLAKGDAAAGSILVILLE
                     KGGNPRLFERLLQPDGCYSWQESGSQRIENPREVPEFIARRRRFDPDLWVLELDIP"
BASE COUNT          667 a         1605 c         1556 g          698 t
ORIGIN      
        1 cgtcacccgg cgctcgatcg aggagaccgc cttcgcgatc gtcaaattgt gcaacgagcg
       61 caccgacgcc gcggccgccg cggtggcggg ggcgtgaggc tcctgctcgc ctcgaagagc
      121 gaggcgcgcc ggcgcatgct cgaagcggcc ggggtgcggt tcgagacggt cgaggcggag
      181 ctcgacgaag gggccgccaa ggccggcctt tggggcgccg gtttcgatgc gcgcggagtc
      241 gccgaggaac tggcgcagct caaggcgctg tcggtcgagg ccggcgagga ttgcctcgtg
      301 ctgggctccg accagacgct cgagacggcg gacggcaccc tgctcggcaa gccggcctcg
      361 cgcgaggagg cctggggcca gctctccctg ctgcgcggcg ccacccatca tctccattcg
      421 gcggccgtgg tcgccgaggc gggcgaggcg gtgtggtgga attgcgagac cgtgtccctc
      481 tccatgcgca gcttcagcga cgagttcctg cacgcctatc tggacgccga atgggagcag
      541 atccgctgga gcgtcggcgg ttaccggatc gagggccccg gcgcgcaatt gttcgagcgg
      601 gtcgagggca gccagttcgc catcctcggc ctgcccctcc ttcccctgct cggctatctg
      661 cgcgagcggg ggctgctgaa gagctgatgg gcattcccta tgccgaggtg atcggcgatc
      721 cggttgccca ttcgaaatcg ccgctcatcc ataatttctg gctccggcgg atgaacctgg
      781 tcggggacta caggcggacc cgggtaggag ccgaggcgct tggcgactat ctcgccgagc
      841 gcagaggcga ccccgattgg cgcggctgca acgtcaccat gccctgcaag gaggcgatcg
      901 ccgcgcatct cgacacggtc gatccggtcg cccgccgaat cggcgcgatc aacaccgtcg
      961 tccctcgttc cgccgggctg ctgatgggca ccaacaccga tttccagggg gcccacttcg
     1021 ccctttgcga ctgcgcgccg gccggcagca aagcggtgct gctcggcacc gggggcgcgg
     1081 cccgggccgt actcgaagag ctgcgccagg cgggcgccgc gcatgtcacc atcctcagcc
     1141 gcgccgagga gaaggcgcgc aggctcctgg acagcttcgg cgtcgacggc gcagtcggcc
     1201 cgatcggcgg ggcgccggtc gcggacctgc tgatcaacgc gactccgctc ggcatggagg
     1261 gctggccgcc cctcgacctc gacctgtccg ggctccgcgc tggagcgacg gtgttcgacc
     1321 tcgtctatca tccgatcgag actccgctgc tggccgaggc ccggcggctc aggcttcgga
     1381 cgatcgacgg cctcgccatg ctgatcgagc aggcggccat ggccttcacc tatttcttcg
     1441 gcgaaccgcc gccggaccgc tattcgccgg agctgaggga gctgctgacg cgatgatcac
     1501 cctcggcctc accggctcga tcggcatggg caagtcgacc gtcgccagga tgttcgcgga
     1561 cgagggcgtg cccgtcttcg acgccgacgc ggccgtccac cgcctgcagg ggcgggacgg
     1621 ggcgctggtg gaggcgatcg aggcccattt cccgggcacc accgggaccg agggcgtcat
     1681 tcgcggcgct ctggcggaac gggtgctcgg cgagcccgag gcgctccggc ggctcgaggc
     1741 cctggtccac ccggcggtag ccgccgagcg gcaagccttc ctcgccgcca atagaggcag
     1801 tccgctcgtc gtcctcgaca tcccgctcct gttcgaggcg ggcggcgcgg atcaggtgga
     1861 caggatcgcc gtcgtctcgg cgccggcgga ggttcaacgg cagcgcgtcc tctcccgtcc
     1921 cgggatgacg ccggaaaaat tcgagcggat cctggagcgc cagctccccg actcggagaa
     1981 gcgcgcccgc gccgacttcg tcattcccac cgggggctcg ctggaggaga cgcgacgagc
     2041 ggttcgccgc atcctcgctt gcctcgtccc gcccgccgat tcatagtcct ggccatgcgg
     2101 gaaatcgtct tcgacaccga aaccacgggg ctcagtccgc tcgccggcga tcgcatcgtc
     2161 gagatcggct gcgtcgagct tgtgaaccgc gtcgaaaccg gccgtacgtt ccacgcttac
     2221 ttcaacccgg accgtccgat gccgagcggc gcggaggcgg tgcacggcct gaccgacgtc
     2281 ttcctggcgg acaagccgcg attccacgac cgggtcgagg acctgctcga attcctcggc
     2341 gactgcccgc tggtcgcgca taacgccggc ttcgatttcg gcttcctcaa ccacgagctc
     2401 aacaattgcg gccggccgct gatctgcctg acccggatgg tcgacacgct gcagctggcg
     2461 aggagccggc accccggcgc caagcacagc ctggacgcct tgtgcacccg cttcggcgtc
     2521 gaccgcagcc tccgggtgaa gcacggcgcg ctgatcgacg cccagctgct cgcccaatgc
     2581 tatgtcgagc tgacgggcgg ccgccagatc ggcctcaccc tcaccgacat cgtcgcggag
     2641 ccgatcgagc gggacgcgcc ggccggtccg gaccgttccc gcgacgtcct catcgagatc
     2701 cgcccgccgc gggcgcacgc ccccaccgag gaggagctgc aggcccatgc cctcttcctc
     2761 accaccctcg ccaaccccat atggcaccgg ctggaagggc gcggttgacg taaagccgcg
     2821 gcggagccta atccgcgcgg ctccttgctg gagaaagaaa tggatatccg cgtctcgggc
     2881 catcaggtcg acaccggtga agctttgcgc gagcgtgtgg gcactcggct gaccgccgtg
     2941 gccgacaaat attcgtcgcg actcgtctcg gcccaggtga ccttcggcaa ggggccgcac
     3001 gatcatggct tcacctgcga gatcctggcc tacgctccgc aaggcgtggt gctgaaggcg
     3061 gcggaccgcg ccgccgcggc gcccgccgcc ttcgacgcgg cggccgagaa gatcgagaag
     3121 cagctgcggc gtcacagccg gcgcctcaag gaccatcacc aggacgccgc ggccgagctg
     3181 gaggcggccg agggctacgg ataccgacga ggcgagcccg agccgcaggg cgagtgactt
     3241 cgcgggcatc ccccgcgctt ccttcctatc ccaagcgcct gccaggcgcg gccgcatccg
     3301 gtggaccgat gaacgacatg accgacatcc tcaccgtcga ccgagtcgat gccgggatga
     3361 ccgttccgaa caagaagggg ctgttccagc tgctggcggt cgctgccgca cgccggacgg
     3421 ggctcgattc gaagacgatc gcggccgcgc tcagcgagcg ggagaagctg ggctcgaccg
     3481 gcttcggcgg cggagtcgcc attccccatg cgcggctcgc ggggctgaac ggcgtgttcg
     3541 gctatttcgc ccgcctggcg gcgccggtcg acttccagtc ggtcgacacg ttgccggtcg
     3601 acctcgtctt cctgatcctg tccccgccgg acgcgggggc cgaccatctg aaggcgctgg
     3661 cggcggtgag ccgggtgctg cgcgaccgca acacggtggc gaagctgcgc ggcgcgcggt
     3721 cgcgcgacgc gctgttcgcc ctgctctccg gcgtcgaaag ccgcgatgcc gcctgatccg
     3781 gaggccggcg cggccgccca tttccgggcg ctcgaatcgc tctaccgaca cgcccccgtc
     3841 aacggcctgt tcgaatcgcg tctcgagatc gttgagaagg gctttgcgcg catccgcttc
     3901 gaggtcggcg agccgctgtt ccacgccgcc ggcgccgctc acgggacgct ctatttcaag
     3961 atgatggacg acgcggcctt ctacgcctgc aacagcctcg tcaccgaccg cttccttctg
     4021 accaccgcct tcaacctcct cttcacccgc ccgctccgcg ccgggacggc gacggcggag
     4081 ggccgctggg cgagcggacg ccggcgagtc ctggtcgggg aggcgcggct gctcgactcg
     4141 gacggccagc tcgccgccag cggcaccggg accttcatgc gctcgcacat ccccttgagc
     4201 ggcctgcccg gctattcggc gggctgatgg agccccggat cgccgcatcg gtcctggccg
     4261 gagcgctgat tcgcgcggcg gagggcgagg ggggcttcgg cgccgtcctc gcgaagggcg
     4321 atgccgcggc cggctcgatc ctcgtcatcc tgttggaaaa aggcgggaat ccccgcctct
     4381 tcgaacgcct gctccaaccc gacggatgct attcctggca ggagagcgga agccaaagga
     4441 ttgaaaatcc tcgtgaagtt ccagagttca tcgctcggcg gaggcggttc gacccggatt
     4501 tgtgggtcct cgaactggat atcccg
//
LOCUS       DASVQZ010000036         3349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_393262, whole genome shotgun sequence.
ACCESSION   DASVQZ010000036 DASVQZ010000000
VERSION     DASVQZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393262"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VF547_00995"
     CDS             <1..668
                     /locus_tag="VF547_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261430.1"
                     /translation="PVIVYFYGGSWNSGRRQGYAFAGRALAARGFVVVVPDHRLVPEA
                     RYPEFLRDCAAAVRWARRHASRFGGDGGRIVLVGHSAGAYNAAMLALDPSLLGPDRAA
                     IRGFAGLAGPYDFLPLDDPSTIAAFGKWPRPAETQPVRHAGPGAPPALLLHGADDVRV
                     KPRNSRILAERLAAAGGDARLTLYPRLGHVAILTALAVPFRGRAPVLADVAGFARRAA
                     GED"
     gene            674..934
                     /locus_tag="VF547_01000"
     CDS             674..934
                     /locus_tag="VF547_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261431.1"
                     /translation="MDLDAQLRNYFGTGDPAAIAPAALAAGIERMRVDLGLEKDRGNR
                     FALWALLHMLGAAPDLDIAFEDERDREAARNFMDLADRSGDG"
     gene            complement(965..2302)
                     /locus_tag="VF547_01005"
     CDS             complement(965..2302)
                     /locus_tag="VF547_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8261432.1"
                     /translation="MARKRRLWRSLYFQVLVAIALGVLVGWQFPDLGASLKPLGVAFI
                     KLVKMIITPVIFLTVTTGIAGMSDLKAFGRVGAKAMAYFLTFSTLALAVGLAVANVVR
                     PGDGLNVDPAALDQGAVSTYVDKAHDQSITGFLLDIVPDTFFSAFTSGQILQVLFVSI
                     LFGVALALLGRRGEPLLDGFRILTAVMFRIVHILMYAAPVGAFGAMAYTIGAHGIRSL
                     ANLAALIATFYATSLLFVLVVLGLAARAAGFSIFALIRYLKDELLLVLGTSSSESAMP
                     LLMEKLERAGCPKPIVGLVVPTGYSFNLDGTNIYMSLAALFIAQATNTPLSFADQMLL
                     MAVAILSSKGAAGVTGSGFIVLAATLSVLPTVPVAGMALILGVDRFMSECRSLTNFIG
                     NAVATIVVARWERAVDLRRLEAAFAGRGGDFEEAESDALQIEGVSDVTPQAPR"
     gene            2745..3311
                     /locus_tag="VF547_01010"
     CDS             2745..3311
                     /locus_tag="VF547_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8261433.1"
                     /translation="MKKIALLVSAVALPLAVPGVAHAQETTGARPYIGVQAGLHNLNV
                     DEDEIDTAGFDLDDSALTYGLYGGVDFDLGKSAVIGVEGNFNLGNGPIDSEYGIAGRI
                     GFRAKGGTVIFARAGYQWIDIDGAGLIGVDEDLIDDDDLDVSDTVGDFLVGAGADIAV
                     GESMGIRVAVDTVSFDTLRPSVGLHLRF"
BASE COUNT          529 a         1188 c         1136 g          496 t
ORIGIN      
        1 ggccggtgat cgtctacttc tacggcggct cgtggaacag cggccgccgc cagggctatg
       61 ccttcgccgg ccgggccctg gccgctcggg gcttcgtcgt cgtcgtcccc gaccaccggc
      121 tggtgcccga ggcgcgctat cccgaattcc tgcgcgattg cgccgcggcc gtgcgatggg
      181 cgcgccggca cgcgagccgg ttcggcggcg acggcgggcg gatcgtcctc gtcggccatt
      241 ccgccggcgc ctacaacgcc gccatgctgg cgctcgaccc aagcctgctc ggcccggacc
      301 gggccgcgat ccgaggcttc gccgggctcg ccgggcccta cgacttcctg ccgctcgacg
      361 atccgtcgac gatcgccgcc ttcggcaaat ggccccggcc ggccgaaacc cagccggtcc
      421 gccacgccgg gccgggcgct ccgccggcgc tgctcctcca cggcgccgac gatgtccggg
      481 tcaagccgcg caacagccgg atcctcgccg agcgtctggc cgcggcgggc ggcgacgccc
      541 ggctgaccct ctatccgcgc ctcggccatg tcgccatatt gaccgcgctc gccgtcccgt
      601 tccgcgggcg cgcgccggtc ctcgccgacg tcgcaggctt cgcccgccgg gcggccgggg
      661 aggattaggc cggatggatc tcgacgccca gctccgcaac tatttcggca ccggcgatcc
      721 ggccgcgatc gcgccggccg cgctcgccgc ggggatcgag cggatgaggg tcgacctcgg
      781 cctggagaag gaccggggca accgcttcgc gctctgggcc ctgctgcaca tgctcggcgc
      841 cgcgcccgac ctcgacatag ccttcgagga cgagcgcgac cgcgaggcgg cgcgcaactt
      901 catggacctg gcagaccgct cgggggacgg ctgacgcgag cgcccgcgcg cggtccgccc
      961 ggcctcagcg aggggcctgc ggtgtgacgt cgctgacgcc ctcgatctgg agggcgtcgc
     1021 tttccgcctc ctcgaaatcg ccgccccgcc cggcgaacgc cgcctcgagc cggcgaaggt
     1081 ccaccgcccg ctcccagcgc gccacgacga tggtggcgac ggcattgccg atgaagttgg
     1141 tcagcgagcg gcattcgctc atgaaccggt cgactcccag gatcagcgcc attcccgcca
     1201 ccggcacggt cggaagcacc gacagggtcg ccgccagcac gatgaagccg gagccggtga
     1261 ctcccgcggc gcccttcgag ctcaggatcg cgaccgccat cagaagcatc tggtcggcga
     1321 agctcagcgg cgtgttcgtc gcctgggcga tgaacagggc ggcgagcgac atgtagatgt
     1381 tggtgccgtc gaggttgaag ctgtagccgg tcggaacgac cagcccgacg atcggcttcg
     1441 ggcagccggc gcgctcgagc ttctccatca gcagcggcat cgcgctctcg gagctcgacg
     1501 tcccgaggac gagcagcaat tcgtccttga gatagcggat cagggcgaag atcgagaagc
     1561 ccgccgcccg cgccgccagg ccgaggacga cgaggacgaa cagcagcgag gtcgcgtaga
     1621 aggtcgcgat cagcgccgcc agattggcca agctgcggat gccgtgggcg ccgatcgtat
     1681 aggccatggc cccgaacgcg ccgaccggcg ccgcgtacat caatatgtgg acgatccgga
     1741 acatgaccgc ggtcaggatc cggaaaccgt cgagcagggg ctcgccgcgc cggccgagca
     1801 gggccagcgc gaccccgaac aggatcgaga cgaacagcac ctgcaggatc tggcccgagg
     1861 tgaaggcgct gaagaaggtg tcggggacga tgtcgagcag gaagccggtg atcgactggt
     1921 cgtgcgcctt gtcgacatag gtcgagaccg ccccctggtc gagcgcggcc gggtcgacgt
     1981 tcagtccgtc gcccggcctg acgacattgg cgacggccag cccgaccgcc agcgccagcg
     2041 tcgagaaggt caggaaatag gccatggcct tggcgccgac ccgcccgaac gccttcaagt
     2101 ccgacatccc ggcgatcccg gtggtgacgg tgaggaagat gaccggggtg atgatcatct
     2161 tgaccagctt gatgaacgcg acgcccagcg gcttcagcga cgcgccgaga tcggggaact
     2221 gccagccgac cagcacgccg agcgcgatgg cgacgagaac ctggaagtag aggctgcgcc
     2281 ataggcgtct tttccgagcc acgtgcttct ccgtcgggct ccgctatggc cgagcgatcc
     2341 tcgacgctag cgcgcccggg gaggctagtc acgggtccct ggcggaagcg gcccgttgaa
     2401 cggggccgcc agccttccgg ctccggcggc accccggccg cgcccgcgat cccgattttg
     2461 tgcggcgcca cgaacggggc ggccgcgggg ccgtatggga gtagtcgtgc atcgcgctga
     2521 ccgcgcaggg cgaatatccg gcccccgtcg ggcccccgcc tattcaaacc caaactgatc
     2581 catgttgcga tagggcctcc cgccggcctt gagcctccac cacctgttgc ttttggaaca
     2641 cgctgttgcg caatcgtcgc ccttgtcttg cgcaggctcg ccgattcatt aagttcacgg
     2701 ccacctggtc gcaagaacgc gacagatgag aagggttcag atttatgaag aagatcgcat
     2761 tgctggtgag cgccgtggct ctgccgctcg ccgttcccgg cgtggcccat gcgcaggaga
     2821 cgaccggcgc tcgtccctat atcggagtcc aggccggtct ccataacctc aacgtcgacg
     2881 aggacgagat cgacaccgct ggtttcgacc ttgacgattc cgctttgacc tacggcctct
     2941 acggcggcgt cgacttcgac ctcggcaagt cggccgtgat cggcgtcgag ggcaatttca
     3001 acctcggcaa cggcccgatc gattccgaat acggcatcgc cggccgcatc ggcttccgcg
     3061 cgaagggcgg cacggtcatc ttcgcccgcg ccggctatca gtggatcgac atcgacggcg
     3121 ccggcctgat cggcgtcgac gaggacctca tcgacgacga cgatctcgac gtctccgaca
     3181 cggtcggcga cttcctggtc ggcgccggcg ccgacatcgc ggtcggcgag agcatgggca
     3241 tccgcgtcgc ggtcgacacc gtgtcgttcg acaccctccg cccgagcgtc ggcctgcacc
     3301 tgcgcttctg agcgcaagca tccgattccg gaaaatcgaa gcggccggt
//
LOCUS       DASVQZ010000037         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_8668,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000037 DASVQZ010000000
VERSION     DASVQZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8668"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="argJ"
                     /locus_tag="VF547_01015"
     CDS             complement(<1..1142)
                     /gene="argJ"
                     /locus_tag="VF547_01015"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX8261434.1"
                     /translation="MSVTAARGFVAAGCHAGIKRRRFDMALVATDDRRPVAAAAVFTR
                     NKFVAPPVILSRRRLAENGGRAAAIIVNSGNANAGTGAAGLADAEAMAAETARALDVP
                     AEQVLVCSTGIIGTPLPMDVILAATPKLARKLSAGGAHDAARGILTTDHMAKETVVKG
                     STFTLGGMAKGCGMIAPDMATMLAFLTTDAEVSAEEMTAILRDSSDRTFNTLNVDGAT
                     STNDSAMLLASGRRGRPDLSEFADAVHEACEALTLQMARDAEGMTKMVALSVTGAASD
                     AEARIAAKSIAENNLVKCSWYGGDPYWGRLLAAAGSAGIAFEPEKTAVAYGGTVVARG
                     GANAEHDADAVAEHMKGERIEIEVALGLGTGRAQVIGIDLGPGYIK"
     gene            complement(1240..2241)
                     /gene="argC"
                     /locus_tag="VF547_01020"
     CDS             complement(1240..2241)
                     /gene="argC"
                     /locus_tag="VF547_01020"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX8261435.1"
                     /translation="MIRVAILGASGFVGAELLRLCAQHPAFSPGKLFGSSRAGLGLAE
                     VHPHLALAYPQATVNSYAAEALDGVDLVFAALPHGQSQALAQDVLGRGIPFVDLGADF
                     RLGSAEDYRRWYREPHGAPELLCRFVYGIPELDRASIAGSAAVAAAGCYATAAILALK
                     PLMDLIEPGSVVVDAASGVSGAGKGLKEETHFNSVAESFSAYGLVGHRHTAEMEMALG
                     GTVLFTPHLAPMNRGILATCYAAAKGPCDPLEALLEAYAGEPFIHVSERPPATKWVLG
                     SNAVHLTARYDERTGRVLAIAALDNLVKGAAGQMIQCANLMLGLEETAGLPVAGVYP"
     gene            complement(2289..2489)
                     /locus_tag="VF547_01025"
     CDS             complement(2289..2489)
                     /locus_tag="VF547_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261436.1"
                     /translation="MDAMQDVMIDALQIVGVCLLIAIIAVALAVFRPTARRRRRRKRH
                     GQRNRIDLFAPARTEAPTEPDA"
     gene            complement(2518..>3346)
                     /locus_tag="VF547_01030"
     CDS             complement(2518..>3346)
                     /locus_tag="VF547_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8261437.1"
                     /translation="RRAADLRIVRRLEHRGGAAAVAFAPDGKTLATSGYDGAVRLWDV
                     AGGRLVRTLAGAAGTVWTIDYAPDGRRLAAAGEDGLVRVWSPSDGRLLLTLRGHSRNV
                     WEVRFSPDGRRLASGSFDSTARLWDAGTGIALATLSDHDEAVVGLAWSPDGRWLATGG
                     DDSTIRLRRAADGATIRRIDVGNHAYKLAFSPDGRWLANAGRARGGAGTFWHGLTGAG
                     GAGEPVHLWRTADGALVQVLTGPEDMMYVGFSPDGRWLVTSADDGVVTLWRLRLGRA"
BASE COUNT          467 a         1184 c         1212 g          483 t
ORIGIN      
        1 tctttgatgt agccggggcc gaggtcgatc ccgatcacct gcgcccgccc cgtcccgagg
       61 ccgagcgcga cctcgatctc gatccgctcg cctttcatgt gctcggccac ggcgtcggcg
      121 tcgtgctcgg cgttggcgcc gccgcgcgcg accaccgtcc cgccgtaggc gaccgccgtc
      181 ttctccggct cgaaggcgat cccggccgag cccgccgcgg cgagcagccg gccccaatat
      241 gggtcgccgc cataccagct gcatttgacg aggttgttct cggcgatcga cttggccgcg
      301 atccgggcct cggcgtccga cgcggcgccg gtcacggaca gcgcgaccat cttggtcatg
      361 ccctcggcgt cgcgggccat ctgcagggtc agcgcctcgc aggcctcgtg taccgcgtcg
      421 gcgaactcgg acaggtccgg cctgccgcgg cggccggagg cgagcagcat cgcgctgtcg
      481 ttggtcgagg tcgctccgtc gacgttcagc gtgttgaacg tccggtccga agagtcgcga
      541 aggatcgccg tcatctcctc ggccgagacc tcggcgtcgg tggtcaggaa ggcgagcatc
      601 gtcgccatgt cgggggcgat catcccgcag cccttggcca ttccgccgag ggtgaaggtc
      661 gagcccttca ccaccgtctc cttggccatg tggtcggtgg tgaggattcc ccgggccgcg
      721 tcgtgggcgc cgccggcgga cagcttcctg gcgagcttcg gggtcgcggc caggatcacg
      781 tccatcggca ggggcgtgcc gatgatgccc gtcgagcaga ccagcacctg ctcggccggg
      841 acgtccagcg cccgcgccgt ctcggccgcc atcgcctcgg cgtcggcgag gccggcggcg
      901 ccggtgccgg cattggcgtt gccggaattg acgatgatcg ccgccgcccg gccgccgttt
      961 tcggcgagcc gcctgcgcga caggatcacc ggcggagcga cgaacttgtt gcgggtgaac
     1021 accgccgccg ccgcgacggg gcggcggtcg tcggtggcga ccagtgccat gtcgaagcgc
     1081 cgccgcttga tgccggcgtg gcagccggcg gcgacgaatc cccgggccgc ggtgacgctc
     1141 acttcgaatt cctcccctgc ttttgcaggg cagggggacc aggcgaagcc tggcggaggg
     1201 gctctttcca atcccggaga ggaatttcgc tggccgccct cacggataga cccccgcgac
     1261 cggcagcccg gcggtctcct cgaggccgag catcaggttc gcgcactgga tcatctggcc
     1321 ggcggcgcct ttgacgagat tgtcgagcgc cgcgatcgcc agcacccggc cggtgcgctc
     1381 gtcgtagcgc gcggtgaggt ggacggcgtt ggagcccagg acccatttgg tcgcgggcgg
     1441 tcgctccgag acgtggatga agggttcgcc ggcataggct tcgagcaacg cctccagcgg
     1501 gtcgcagggt cccttggcgg cggcgtagca ggtggcgagg atgccgcggt tcatcggcgc
     1561 gaggtgcgga gtgaacagca cggtcccgcc cagcgccatc tccatctcgg cggtgtgccg
     1621 gtgcccgacc aggccgtagg cggagaagct ctcggcgacg ctgttgaaat gagtctcctc
     1681 cttgagcccc ttgccggcgc cgctgacccc ggaggccgcg tcgacgacga cgctgcccgg
     1741 ctcgatcaga tccatgagcg gcttcagcgc caggatcgcc gccgtggcgt agcacccggc
     1801 cgcggccacc gccgccgagc ccgcgatcga tgcgcggtcg agctccggaa tgccgtagac
     1861 gaaccggcag agcagctccg gggcgccgtg cggctcccgg taccagcgcc gatagtcctc
     1921 cgccgagccg aggcggaaat cggcgccgag atcgacgaag gggatgccgc ggccgagcac
     1981 gtcctgagcg agcgcctgcg actggccgtg ggggagggcg gcgaagacga ggtcgacgcc
     2041 gtccagggcc tccgctgcat aactattcac ggtcgcctgc gggtaggcga gcgccagatg
     2101 ggggtggacc tcggcgagtc cgagccccgc ccggctgctt ccgaaaagct tgccgggact
     2161 gaaggccggg tgctgcgcgc agagcctgag aagctctgca ccgacaaagc ccgaggctcc
     2221 gaggatggcg acgcgaatca tccgggcgcc atgcgcgtta ctgcatagtt atgcaagaac
     2281 cttcagaatt atgcatcggg ctccgtgggg gcttccgtcc gcgccggggc gaacaggtcg
     2341 atccggttgc gctgcccgtg ccgcttgcgc cgccgccgcc gcctcgccgt cgggcggaag
     2401 acggccaggg ccacggcgat gatcgcgatc agaaggcaga cgccgacgat ctggagcgcg
     2461 tcgatcatga cgtcctgcat cgcgtccacc tccctcgccg gagcttaggc catgcgccta
     2521 ggccctgcca agccgcagcc tccacagcgt gacgacgccg tcgtcggcgg acgtgaccag
     2581 ccagcgcccg tcggggctga agcccacata catcatgtct tcgggcccgg tcaggacctg
     2641 caccagcgcg ccgtcggcgg tcctccacag atggaccggc tcgccggcgc cgccggcgcc
     2701 ggtgaggccg tgccagaacg tacccgcccc gccccgggcg cggccggcat tggccagcca
     2761 gcgcccgtcg gggctgaagg cgagcttgta ggcatggttg ccgacgtcga tccgtcggat
     2821 cgtcgctcca tccgcggcgc ggcgcaggcg gatggtggaa tcgtcgccgc cggtggcgag
     2881 ccagcgtccg tcggggctcc aggccaggcc gaccacggcc tcgtcatgat cggagagggt
     2941 ggcgagcgca atgccggtgc cggcgtccca gagccgggcg gtcgaatcga agctgccgct
     3001 cgcgaggcgg cgtccgtcgg ggctgaagcg gacctcccag acgttgcgcg aatggccgcg
     3061 gagcgtgagc aggaggcggc cgtccgaagg gctccagacg cgcacgagtc cgtcttcccc
     3121 cgccgcggcg agtcgacgcc cgtcgggcgc atagtcgatc gtccacaccg tcccggcggc
     3181 gcccgcgagc gtccggacga ggcgcccgcc ggccacgtcc cacagccgga ccgcgccatc
     3241 atagccggac gtcgcaagcg tctttccgtc gggcgcgaag gcgacggcgg cggcgccgcc
     3301 gcggtgctcc aggcggcgaa cgatcctgag gtccgcagca cggcgg
//
LOCUS       DASVQZ010000038         7522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_201993, whole genome shotgun sequence.
ACCESSION   DASVQZ010000038 DASVQZ010000000
VERSION     DASVQZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201993"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..326)
                     /locus_tag="VF547_01035"
     CDS             complement(102..326)
                     /locus_tag="VF547_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261438.1"
                     /translation="MDDGKSSDDKLGGRGGGGDSGGGAYPNPHRGKAGYKDGFLGGGG
                     QTERSYHGHGRLGDETVAENPNAPAEDEDD"
     gene            complement(396..1073)
                     /locus_tag="VF547_01040"
     CDS             complement(396..1073)
                     /locus_tag="VF547_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8261439.1"
                     /translation="MVETDVEIRRSVVINLASIGAEAWPFGGGGEFLEILGHLMPGCV
                     LLDMDAPDGIETLSALVRRETGWPILAMSGRPDVSLAVETMKLGALDFLEKPVDAAKL
                     AAALVPAWTALEASLERGEARRIAQARLARLTARELDISLALFGGRSNKAVAHDLGIS
                     VRTVEMHRAHIMAKLGVKSIAEAAVMATHAGLAIAQRKPVASVLGPVGLGRPPSPFDG
                     RRLARSA"
     gene            1431..1967
                     /locus_tag="VF547_01045"
     CDS             1431..1967
                     /locus_tag="VF547_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX8261440.1"
                     /translation="MPVASAGDGLVVEVAHIFSGNALGALDEDGRVHLPPFILREVSR
                     HGDGGRLLLGVHEKDPCITGYGSALRPRLEAELERRRLREEALGLPPDDHHARARRTF
                     GLVEEALYDDGGRLTLPPFARRRGGFGRDILFIGAGSHFEIWDPSVALAEGDDELRFV
                     AQYRLGGTDRGFQEEEGR"
     gene            1964..2389
                     /locus_tag="VF547_01050"
     CDS             1964..2389
                     /locus_tag="VF547_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261441.1"
                     /translation="MSLLCALGGHEAGAREIYNGGYYFARCRRCERDMIRCGAAWQMV
                     PSGHRVVWRTGRHQHSLEPDLSGALPVLHREANLPAVRPPFASWSRDMVKLRRAPAAA
                     ASAAAAVVEREEPHYPRLLVFAALVGAGLQLIFSLGSAR"
     gene            complement(2405..4141)
                     /locus_tag="VF547_01055"
     CDS             complement(2405..4141)
                     /locus_tag="VF547_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8261442.1"
                     /translation="MAGTDRPPGDGNFLIVAPTGRDSEVIGQLLRSASLPFAIDRGGD
                     RLFAAIASGGEAGAIVTDDALARLDTERLRAAIERQPPWSDFPFVLLVRRGETREGSR
                     AMEGLINVTVLERPLHPATLISAVRSAIRGRRRQRLAADYLAEREAAEARLRELAETL
                     EQKVDERTRDLAAANDRLTAEIAERERAEARLVQAQKMEAIGQLTGGVAHDFNNLLTA
                     IVGSLELLLRRTDDERLRRLASNALQAAERGARLTTQLLAFSRRQRLTPTPLDVNRVI
                     AGMIDLLTRSIGPQVKVETRLEPDLSRALADPTQLEVMILNLAINARDAMPAGGSLVI
                     ETRDLAAVPEPLAGELQPGRYVAICVSDTGIGMTPEILQHAFEPFFTTKAQGKGTGLG
                     LAQLYGFARQSGGTARIESEEGRGTSVIIYLPATEAEPELAKSAPAERLRLRRGRILV
                     VDDDDDVRQVAAAMVAELGYSVAVAADAEQALRLLEAGRFDLMVTDVAMPGTNGVELG
                     RQARLVAPDMPILFASGYADVQTFGAELLDEAVLRKPFRIAEVAARIHHLLEDGPPPA
                     GAGLYAVPIDRD"
     gene            complement(4125..5639)
                     /locus_tag="VF547_01060"
     CDS             complement(4125..5639)
                     /locus_tag="VF547_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX8261443.1"
                     /translation="MADSDSAPMDSGAVPSGSEPLDYILGGGYASSHIHLLEGAPGSG
                     KTTLGLQFLLHGKARGEACLYITLSESRDELLQAARTHGWDLSGIEIFELVPPELSLD
                     PDREQSIVYASDLELGETVRLVMEEVERLAPARVVFDSLSEIRVLAQGSLRYRRQVLA
                     LKHFFARRRCTALFLDDLTDVAEDLSLHSLAHGVVRLEQIAMSYGAERRRLRVFKMRG
                     RRFHGGFHDFVIRSGGVEIFPRLVAADHPDTGFEQRAATSGLPELDELVGGGLDYGTT
                     TLVIGPSGSGKSTLSLQFMKSALARGEKVLVVSFDETERVFFRRATGLGMDLTHATED
                     GTFIFRQVDPAELSPGELTGIIREHVEALGASVVVVDSLSGYQHAMPQEQHMLLQMHE
                     IVTYLNQQGVLTFLILAQSGMVGHMQTPVDLTYLSDNVLLLRFFEAHGEIRRALSVLK
                     KRTGGHESAIRELKIGSGGIEVGPRLTGFRGVLTGTPSYEGQSALLEDGIGRNR"
     gene            5776..6450
                     /locus_tag="VF547_01065"
     CDS             5776..6450
                     /locus_tag="VF547_01065"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX8261444.1"
                     /translation="MKPMGEQHLAVLRRHMVEIVDLHFDLASDELGKDSLDPALRAAL
                     LRVPRHLYVPHQLAVAAYQDTPLPIGFDKTISQPFIGALMLDLLELRPGQRVLEVGTG
                     LGWQAAVMSALGAQVWSVEIVEEFAEGARLRLAAEGHDVQIRVGDGSRGWSDHAPFDR
                     ILVTAAAPVPPPALAAQLAPGGRIVIPLGGKDVQQLSVVAKAAGGSLGVREVLPTRFT
                     QLETAT"
     gene            6636..>7522
                     /locus_tag="VF547_01070"
     CDS             6636..>7522
                     /locus_tag="VF547_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEX8261445.1"
                     /translation="MLRVYACIAGEHDIRLVLVAGTICLLAAFTAFSIFEQARRAETR
                     RWAWTSLAGFVAGTGIWATHFVAMLAYRPNLPIGYDLELTLASIAAAVLITGAGWTAA
                     LRRGRAGTLAGGAAIGAGIGTMHYIGMAAVDLPARFVWDEAMVAASLLIGAALSVAAL
                     AEHRRRPQALPWRPALLFTLAICGLHFTAMAAASIYPDPRLGVPSSAIDGAVLTLAVV
                     AMALVILAIGFLMVLFDRKLAKNAIEEARRLRTFADAAVEGLVVIDGERIVDANRSFL
                     ALAGFDSLGDAPERLSDLL"
BASE COUNT         1089 a         2638 c         2638 g         1157 t
ORIGIN      
        1 cctgtcgaag ccctcccctc cacccgtcag cggcataaac aagaagaggg cttcgacaag
       61 ctcagcccga acggcgaaat tcggagccga ccctccggga ctcaatcgtc ctcgtcctcc
      121 gcgggggcgt tgggattttc ggcgaccgtc tcgtcgccga ggcggccgtg gccgtgatag
      181 gagcgctcgg tctggccgcc gccgccgagg aagccgtcct tgtagccggc cttgccccga
      241 tgcgggttcg gataggcgcc gccgcccgaa tcgccgccgc cgccgcgccc gcccagcttg
      301 tcgtcgctgc tcttcccgtc gtccatggcg cgctcctcga agccggttcg gtcacccggc
      361 ctgaatgagc cgcgtcgccc gaaggttccg gaaagctagg cggaccgggc cagccgccgc
      421 ccgtcgaacg gcgaaggggg gcggcccagg cccaccggcc cgagcaccga ggcgaccggc
      481 ttgcgctggg cgatggcgag gccggcatga gtcgccatca ccgccgcctc ggcgatgctc
      541 ttgacgccga gcttggccat gatgtgggcg cgatgcatct ccaccgtccg gacgctgatg
      601 cccagatcgt gcgccaccgc cttgttcgac cggccgccga acagggcgag cgagatgtcg
      661 agctcgcggg cggtgaggcg ggcgagccgc gcctgggcga tccgacgcgc ctcgcccctt
      721 tcgagcgacg cctcgagcgc cgtccaggcc gggacgagtg ccgccgcgag cttggcggcg
      781 tcgaccggct tctcgaggaa atcgagggcg ccgagcttca tcgtctcgac ggcgagcgag
      841 acgtccggcc gcccgctcat ggcgaggatc ggccagcccg tctcgcgccg gacgagcgcg
      901 ctcagcgtct cgatcccgtc gggcgcgtcc atgtcgagca ggacgcagcc cggcatgaga
      961 tggccgagaa tctcgagaaa ctcgcccccg ccgccgaacg gccaggcctc ggcgccgatc
     1021 gacgccagat tgatgaccac ggaccgccgt atctcgacgt cggtctctac cacatacaca
     1081 gtgcgctggt gcgacataag cccgctcccc caaaggccaa tacacagggc cacgcatggt
     1141 caactttccg agtcgaccgg tgaatatgca acctaggaga attacggtga accgcagctt
     1201 gacaggctgg cgggaaagtc tgcgccttca gcgacttagg taaagggggc cgcctctccg
     1261 gcttggcaat agccgatatg atgcaggtta ggtgcgagga cgatttgcga ccacttcgga
     1321 tggtaattcc aacgccgaat catcgtacca ttaccggtgt acaagtccga tctgcggaaa
     1381 cccgccgtta accattaacc tcctccccag cacggatcgc gcaagcctcc gtgccggtag
     1441 cgtcagcggg ggacggactg gtcgtggagg tcgcgcacat cttcagcggg aacgcgctcg
     1501 gcgcgctcga cgaggacggc cgggtccatc tcccgccttt catccttcgc gaggtgagcc
     1561 ggcacggcga cggcggccgg ctgctcctgg gagtccacga aaaggacccg tgcatcaccg
     1621 gctacgggag cgcgctcagg cctcggctcg aggccgagct ggagcgccgc cgccttcgcg
     1681 aggaggcgct cggccttcct ccggacgacc atcacgcgcg cgcccggcgc accttcggcc
     1741 tggtcgagga ggcgctgtac gacgacggcg ggcggctgac cctgcccccc ttcgcgcgcc
     1801 gccgaggcgg cttcgggcgc gacatcctgt tcatcggcgc cggcagccac ttcgagatct
     1861 gggatccgtc ggtggcactc gcggagggcg acgacgagct tcgcttcgtc gcgcaatacc
     1921 ggctcggagg gacggaccgg gggtttcagg aggaggaagg aagatgagct tgctgtgcgc
     1981 gttgggaggc catgaggcgg gggcccggga aatctacaat ggcggctatt atttcgcccg
     2041 ctgccggcgc tgcgagcgcg acatgatccg ctgcggcgcc gcctggcaga tggtgccgag
     2101 cgggcaccgc gtcgtctgga ggacgggccg gcaccagcat tcgctcgagc ccgatctgtc
     2161 cggcgctctg cccgtcctcc accgcgaggc caatctcccg gcggtgcggc cccccttcgc
     2221 ctcgtggagc cgcgacatgg tgaagcttcg gcgcgccccc gccgcggcgg cttcggcggc
     2281 ggcggcggtc gtcgagcggg aggagcccca ttatccgcgg ctgctcgtct tcgccgccct
     2341 ggtcggcgcc gggctgcagc tgatcttcag cctcggctcg gcccggtagc gcaccggccg
     2401 ggcctcagtc gcggtcgatt gggacggcgt agagaccggc gcccgccggc ggcggcccgt
     2461 cctcgagcag gtggtggatc cgggcggcga cctcggcgat ccggaacggc ttgcgcagga
     2521 ctgcttcgtc gagcagctcc gcgccgaagg tctggacgtc ggcatagccg ctggcgaaca
     2581 ggatcggcat gtccggggcg acgagacgcg cctgccggcc aagctccacc ccgttcgtcc
     2641 ccggcatggc gacgtcggtg accatcaggt cgaagcggcc ggcctcgagc agccgaagcg
     2701 cctgctccgc gtcggcggcg accgcgacgc tgtagccgag ctcggcgacc atcgccgcgg
     2761 cgacctggcg cacgtcgtcg tcgtcgtcga ccaccaggat ccggccgcgc cgcagccgaa
     2821 gccgctcggc gggggccgat ttggcgagct cgggctcggc ctcggtggcg gggaggtaga
     2881 tgatgacgct cgtgccccgc ccctcctcgc tttcgatccg ggccgtgccc ccggactggc
     2941 gggcgaagcc gtagagctgg gcgaggccga ggccggtgcc cttgccctgc gccttggtgg
     3001 tgaagaaggg ctcgaacgcg tgctgcaata tctccggggt cattccgatc ccggtgtcgc
     3061 tgacgcagat ggcgacgtag cgtcccggct ggagctcgcc ggcgagcggc tcgggcacgg
     3121 ccgccaggtc gcgggtctcg atcaccaggc tgccgccggc cggcatggcg tcgcgggcgt
     3181 tgatggcgag gttgaggatc atcacctcga gctgggtcgg atcggccagc gcccgcgaca
     3241 ggtccggctc gagccgggtc tccactttca cctgcgggcc gatgcttcgg gtgagcaggt
     3301 cgatcatccc ggcgatgacc cggttgacgt cgagcggggt gggagtcagc cgctggcggc
     3361 gcgagaaggc gagcagctgc gtggtcagcc gggctccgcg ctcggccgcc tgcagggcgt
     3421 tgctggcgag gcggcgaagc ctctcgtcgt cggtgcggcg cagcagcagc tcgagcgagc
     3481 cgacgatcgc ggtcagcagg ttgttgaaat cgtgggcgac gcccccggtg agctgaccga
     3541 tcgcctccat cttctgcgcc tgcacgagcc tcgcctcggc ccgctcgcgt tcggcgatct
     3601 cggcggtgag ccggtcgttg gcggcggcga ggtcgcgggt gcgctcgtcg accttctgct
     3661 cgagcgtctc ggcgagctcg cgcagccggg cttcggcggc ctcgcgctcg gcgagataat
     3721 cggcggcgag gcgctggcgc cggcggccgc ggatcgccga gcggacggcg ctgatcaggg
     3781 tcgccggatg gagcggccgc tcgagcacgg tgacgttgat cagcccttcc atcgcccgcg
     3841 agccctcgcg ggtctcgccg cgccggacga gcaggacgaa ggggaaatcc gaccagggcg
     3901 gctggcgctc gatcgcggcc cggagccgct cggtgtcgag tcgcgcgagg gcgtcgtcgg
     3961 tgacgatcgc ccccgcctcc ccgcccgagg cgatcgccgc gaacagccgg tcgccgccgc
     4021 ggtcgatcgc gaacggaagc gaagcgctgc gcagcagctg gccgatcacc tccgaatcgc
     4081 gcccggtcgg cgcgacgatc aggaaattgc cgtcgccggg aggcctatcg gttccggcca
     4141 atgccgtcct cgagcaaagc ggactgacct tcgtagctgg gagtgccggt aaggaccccg
     4201 cggaagcccg tgagccgcgg tcccacctcg atgccgccgc tcccgatctt caactcccgg
     4261 atcgccgatt cgtgcccgcc ggtgcgcttc ttgagcaccg acagcgcccg ccggatctcg
     4321 ccgtgcgcct cgaagaagcg cagcagaagc acgttgtcgg aaagataggt gaggtcgacc
     4381 ggcgtctgca tgtggccgac cattcccgac tgggcgagga tcaggaaggt gagcacgccc
     4441 tgctggttca gataggtcac gatctcgtgc atctgcagga gcatgtgctg ctcctgcggc
     4501 atggcatgct ggtagccgga gagcgaatcg acgacgacga cgctggcgcc gagcgcctcg
     4561 acatgctcgc ggatgattcc ggtgagttcc cccggcgaca gctccgccgg atcgacctgg
     4621 cggaagatga aggtgccgtc ctccgtcgcg tgggtgagat ccatgccgag cccggtggcg
     4681 cggcggaaga agacccgctc ggtctcgtcg aagctcacca ccagcacctt ctcgccgcgg
     4741 gccagagcgc tcttcataaa ttgcagcgac agggtcgact tgcccgatcc agacggcccg
     4801 atgacgagcg tggtggtgcc gtaatccagg ccgccgccga ccagctcgtc gagttccggc
     4861 aggccgctcg tcgcggcccg ctgctcaaag ccggtatcgg gatggtcggc cgcgacgagc
     4921 cgggggaaaa tctcgactcc gccgctgcgg atgacgaaat cgtggaagcc gccgtggaag
     4981 cggcggccgc gcatcttgaa cacgcgcagc cgccgccgct cggcgccgta ggacatggcg
     5041 atctgctcga gccgaacgac gccgtgggcg aggctgtgca ggctgagatc ctcggcgacg
     5101 tcggtgaggt cgtcgaggaa cagggcggtg cagcgccgac gggcgaagaa atgcttcagc
     5161 gcaagcacct ggcgccggta gcgcagcgag ccctgggcga gcaccctgat ctcggacagg
     5221 ctgtcgaaca ccacccgcgc cggggccaag cgctcgacct cctccatgac caggcggacg
     5281 gtctcgccga gctccaggtc ggacgcgtag acgatcgatt gctcgcggtc gggatcgagg
     5341 ctgagctcgg gcgggaccag ctcgaatatc tcgattccgg aaaggtccca gccatgggtc
     5401 ctggcggcct ggagcagttc gtcgcggctt tccgacagag tgatgtagag gcaggcctcg
     5461 ccgcgcgcct tgccgtggag caggaactgc agcccgagcg tggtcttgcc cgagccggga
     5521 gcgccttcga gcaggtggat gtggcttgag gcgtagccgc ctccgaggat gtaatcgagc
     5581 ggctccgatc cggacggaac cgcgccggaa tccatgggcg ccgagtccga atcggccatt
     5641 tttctctctc cctcgggcgc gctcactgcc tcattgcagg aacgaaatca aacctttgca
     5701 ggacgacttg aaacggctcg ccctcccttg cgttccggct cgcattctgc gccttgtggc
     5761 cggaaggagg ccctcatgaa accgatgggc gagcagcatc tcgccgtgct caggcgccac
     5821 atggtcgaga tcgtcgacct ccatttcgac ctggcgagcg acgagctcgg caaggactcg
     5881 ctcgacccgg cgctgcgcgc cgcgcttctg cgcgtgcccc ggcacctcta cgtcccgcat
     5941 cagctcgcgg tggccgccta tcaggacacg ccgctcccga tcggcttcga caagaccatc
     6001 tcgcagccct tcatcggcgc gctgatgctc gacctgctcg agcttcggcc cgggcagcgg
     6061 gtgctcgagg tcggaaccgg cctgggctgg caggcggcgg tgatgagcgc gctcggcgcg
     6121 caggtgtgga gcgtcgagat cgtcgaggaa ttcgccgaag gcgcccggct tcgcctcgcc
     6181 gccgagggcc acgacgtcca gataagggtc ggcgacgggt cgcgcggctg gagcgaccac
     6241 gccccgttcg accggatcct cgtcaccgcc gccgcccctg tcccgccgcc cgccctcgcc
     6301 gcccagctcg cgcccggcgg ccggatcgtc atcccgctcg gcggcaagga cgtccagcag
     6361 ctcagcgtcg tcgccaaggc ggcgggcggg agcttgggag tgcgcgaggt ccttccaacc
     6421 cgcttcaccc agctcgagac ggcgacctga gccgccatcg gacccggatc cgttccggct
     6481 gagcttgtcg aagccccacg cgaggaagga aaggccttcg agcccaggcc gaacgggttg
     6541 ggggggcagc cgccgagccg ccccgcccca aatgctttac gccttgttta ctctcccagc
     6601 cccataagcg ccgtttccag gggtgggtcg gacgaatgct gcgggtctat gcctgtatcg
     6661 cgggcgagca tgacatcagg ctggtgctcg tcgcgggcac gatctgcctg ctcgcggcct
     6721 tcaccgcttt ctcgatcttc gagcaggcgc ggcgcgccga gactcgccgg tgggcgtgga
     6781 cgtcgctggc cggcttcgtc gccggcaccg gcatctgggc gacccacttc gtcgccatgc
     6841 tcgcctaccg cccgaacctg ccgatcggct acgacctcga gctgaccctg gcctcgatcg
     6901 ccgccgccgt gctgatcacc ggcgccggct ggaccgcggc gctgcgccgc ggccgcgccg
     6961 gcaccctcgc cggcggggcg gcgatcggcg ccggcatcgg caccatgcac tatatcggaa
     7021 tggccgcggt cgacctcccc gcccgcttcg tgtgggacga ggccatggtc gcggcctcgc
     7081 tgctgatcgg agccgcgctc agcgtcgccg cgctcgccga gcaccggcgc cggccgcagg
     7141 ccctgccctg gcgcccggcc ctgctcttca ccctcgccat ttgcggcctc catttcaccg
     7201 ccatggccgc ggcctcgatc tatccggatc cgcggctggg cgtgccttcc tcggcgatcg
     7261 acggggcggt cctgaccctg gcggtggtgg cgatggcgct ggtcatcctc gccatcggct
     7321 tcctgatggt gctgttcgac cgcaagctgg cgaagaacgc gatcgaggag gcgcggcgcc
     7381 tcaggacctt cgccgacgcc gccgtcgagg ggctggtggt gatcgacggc gagcgcatcg
     7441 tcgacgccaa ccgctccttc ctggcgctcg ccggcttcga cagcctgggc gacgctccgg
     7501 agcgactgtc ggacctgctc at
//
LOCUS       DASVQZ010000039         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221 k141_8934,
            whole genome shotgun sequence.
ACCESSION   DASVQZ010000039 DASVQZ010000000
VERSION     DASVQZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8934"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VF547_01075"
     CDS             <1..938
                     /locus_tag="VF547_01075"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HEX8261446.1"
                     /translation="PVRTGPSWRGAAVYGLTATAVTLAGLGIWTSRGLPQRFSKEVQA
                     SVSDSIPRPVPGAEPCYSTGGFPPKPCSLGPAGAPLLATFLGDSHADAQLLALVEAIP
                     AGARGRVEFNALAGCPPVIDVQPAHSRSRCGAFNARFLAPLTKPRRVPLVLSASWAGY
                     SRKPAFRSSGEEGGGRGQASFHSKLLRSSCALASGGPTYIVLPTPHFPYWVSRELQRR
                     LIADPKAPDLAIPFAAHERRNREVVAVLREAERRCGVRLLDPAPYLCRSGVCPGSLGR
                     RPLYRDDHHLTDGGARLLVPMFRSVFADRTGGGGG"
     gene            complement(959..1138)
                     /locus_tag="VF547_01080"
     CDS             complement(959..1138)
                     /locus_tag="VF547_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261447.1"
                     /translation="MNGAKSDRRSSRAAQQSREIEENQEALRRSIAETERLVSQSEDM
                     LRRHRRERDEDEAAG"
     gene            complement(1158..1334)
                     /locus_tag="VF547_01085"
     CDS             complement(1158..1334)
                     /locus_tag="VF547_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261448.1"
                     /translation="MKLKIDLSCGECGSNRLDIPGQDRDDCPILCEECGHALGSLGDL
                     KARVEQAVLRGGGK"
     gene            1544..1900
                     /locus_tag="VF547_01090"
     CDS             1544..1900
                     /locus_tag="VF547_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8261449.1"
                     /translation="MRILDVDQSPAAEAAALTAHLLAGETVEAAFVAPTGAILFTDRR
                     ILLAQREHLLEARIETSSYPYRAIRHFSIQEGEAAESRSTVRIWLGDEPQPLHLRANP
                     GTDLRPLQRLLADRLS"
     gene            complement(1920..3026)
                     /locus_tag="VF547_01095"
     CDS             complement(1920..3026)
                     /locus_tag="VF547_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX8261450.1"
                     /translation="MPAIRPGLRVRPSRKGRSAFAPPAAIADRLTELGGVRDNPGNLR
                     GRCYVPAGLRGPAPLVVVLHGCTQDAAVYDHGSGWSTLADRHGFLLLFPEQQRANNPM
                     LCFNWFAAGDTRRGKGEAASIRAMIEAMARAHDVDPERIFVTGLSAGGAMASAMLATY
                     PDVFAAGAIIAGVAYGCAADVSEAFDCMGGRARSDSAELGARVRRASSHRGPWPRVQV
                     WQGSADRTVVASNADAIVMQWASLHGLGPHPDRVDSVAGYPRRAWLDSKGEPLVEQYV
                     ITGMAHGIPLAPGDDSLGEAGPHMLDVGLSSTARIAAFFRIAPEAEGGREIEAEPPPA
                     PRRHRPAASAQARPQAGGVQQVIEDALRKAGLMS"
     gene            3225..>3461
                     /locus_tag="VF547_01100"
     CDS             3225..>3461
                     /locus_tag="VF547_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261451.1"
                     /translation="MDAPKGRYSVVEKNGRLVVIDNGTGAPLPSTVAPPPGPRGRAGP
                     APATPISDAGPGPVDRAADLLVALAARRWDPQGRA"
BASE COUNT          451 a         1289 c         1199 g          522 t
ORIGIN      
        1 ggccggtccg gaccggccct tcatggcgcg gagcggcggt ctacgggctg acggcgacgg
       61 cggtgaccct ggccggcctc ggcatctgga cgagccgcgg gcttccccag cgtttctcca
      121 aggaggtgca ggccagcgtc agcgattcga tcccgcgccc ggtgccgggc gccgagccct
      181 gctactcgac cggaggcttc ccgcccaagc cttgcagcct cggccccgcc ggagcgccct
      241 tgctggccac cttcctcggc gacagccatg ccgacgccca gcttctcgcc ctcgtcgagg
      301 cgattccggc cggcgctcgg gggcgggtgg agttcaacgc gctcgccggc tgcccgccgg
      361 tgatcgacgt ccagccggcc cattcgcgga gccgctgcgg cgcgttcaac gcgcgcttcc
      421 tcgctccgct caccaagccg aggcgggtgc cgctggtgct gagcgcgagc tgggccggct
      481 attcccgcaa gccggctttc cgctcctcgg gagaggaagg cggggggcgc gggcaggcct
      541 ccttccactc caaactgctc cgctcctcct gcgcgcttgc ctcgggaggg ccgacctaca
      601 tcgttcttcc gactccgcat ttcccttatt gggtgagccg ggagctgcag cgccggctga
      661 tcgcggaccc gaaggctccg gacctggcga ttccgttcgc ggcgcacgag cggcgcaacc
      721 gcgaggtcgt cgcggtgctt cgggaagcgg agcggcgctg cggagtccgg ttgctcgatc
      781 cggcgcctta tctgtgccgc tcgggggtct gcccggggtc gctcggccgg cgcccgctct
      841 accgggacga tcatcacctc accgatggcg gcgcccggct tctggttccg atgttccggt
      901 cggtgttcgc cgaccggacc ggcgggggcg gagggtaggc cgagccccgg cgggcgcctt
      961 agcccgccgc ctcgtcctcg tcgcgctccc ggcggtggcg gcggagcatg tcctcggact
     1021 ggctgacgag gcgctcggtc tcggcgatgc tccgccgaag cgcttcctga ttctcctcga
     1081 tctcacggct ctgctgggcg gcccggctcg accggcggtc cgacttcgcc ccgttcacgg
     1141 ccgggtttcc aaggggttca cttgcctcca ccgcgaagga cggcctgctc gaccctcgcc
     1201 ttgaggtcgc ccagcgagcc cagcgcatgc ccgcattcct cgcacaggat cgggcagtcg
     1261 tcccgatcct gcccgggaat atccaggcga ttgcttccgc attcgccgca ggacaggtcg
     1321 atcttcaact tcacagccgc ctccgcaacg gcgcgttcta gcctcggtca gcccagctct
     1381 ccttgatttc gatcagggtt gctgccgcaa ttcgcgggtt cacggacggg aagaatagcg
     1441 atctcgaacg cgattcggaa tagccgtgag cggcctcgcc gcccgaatga cgggcccgaa
     1501 ggacgggatt gcctaccgcg cctgccgggc ccatctatcg gcgatgcgca tcctcgacgt
     1561 cgatcagtcg ccggccgccg aagcggccgc gctcaccgcc cacctcctcg ccggggagac
     1621 ggtcgaggcc gccttcgtcg cgccgaccgg ggcgatcctg ttcaccgacc gccggatatt
     1681 gctcgcccag cgcgagcatc tcctggaggc ccggatcgag accagctcct atccctatcg
     1741 ggcgattcgg catttctcga tccaggaggg cgaggccgcc gagagccgga gcacggtcag
     1801 gatctggctc ggcgacgagc cgcagccgct ccacctgcgc gccaatccgg ggaccgacct
     1861 gcgcccgctc cagcgccttc tcgccgaccg gctgagctga aggctcgagc cgcggcggct
     1921 cagctcatca gccccgcctt gcgcagggcg tcctcgatca cctgctggac tccgcccgcc
     1981 tgaggccggg cctgggccga cgccgcgggc ctgtgccgac gcggagcggg cggaggctcg
     2041 gcctcgatct cccggccgcc ctcggcctcc ggcgcgatcc ggaagaaggc ggcgatccgg
     2101 gcggtcgagc tcaaaccgac atcgagcatg tgcgggcccg cctcgccaag cgagtcgtcg
     2161 cctggagcca gcggaatccc gtgggccatt ccggtgatca catattgctc gaccagcggc
     2221 tcgcccttcg agtccagcca ggcgcggcgc ggatagccgg cgacggaatc gacgcggtcc
     2281 gggtgcgggc ctagcccgtg caggctcgcc cattgcatca cgatggcgtc ggcattgctg
     2341 gcgacgacgg tgcggtcggc gctgccctgc cagacctgga cccgcggcca ggggccgcgg
     2401 tgggaggagg cgcggcgcac ccgcgcgccg agctcggccg agtcgctccg cgcgcgtccg
     2461 cccatgcagt cgaacgcctc gctcacgtcg gccgcgcagc cgtaggcgac gcccgcgatg
     2521 atcgcgcccg ccgcgaacac gtccggatag gtggcgagca tcgccgaagc catggctccg
     2581 ccggcgctga ggccggtgac gaagatgcgc tcgggatcga cgtcatgggc tctcgccatc
     2641 gcctcgatca tcgcccggat cgaggccgct tcgcccttgc cgcgccgggt gtcgcccgcg
     2701 gcgaaccagt tgaagcacag catcgggttg ttcgcgcgct gctgctccgg gaacagcagc
     2761 aggaagccgt ggcggtcggc cagggtcgac cagcccgaac cgtggtcgta gaccgccgcg
     2821 tcctgcgtgc agccgtggag gacgacgacc agaggggccg gtcccctcag acccgccggc
     2881 acgtagcagc gcccgcgcag attgccggga ttgtcccgca ctcctcccag ctcggtcagg
     2941 cggtcggcaa tcgccgcggg cggcgcgaac gcgcttcgcc ccttgcggct gggcctgacg
     3001 cgcagaccag gtcgaatcgc aggcatgtcg gtcccccggc ggccggaatg ggcggctcgg
     3061 gggaaacgtc cctccatcgt ttttgttgca gtgcaacata aatcgcggtc ccggcgcgcg
     3121 gcatcggtcc gccttcagct cgcctgtcgg acagcgcaat agcgacgacg gccacttcca
     3181 cgatcaggcg tcccgcctta atctcccctg catcggaggt gaggatggac gcccccaagg
     3241 gacgctacag cgtggtcgag aagaacgggc ggctggtcgt catcgacaac ggcaccggcg
     3301 cgccgctgcc gtccaccgtg gcgccgccgc cgggcccgcg gggccgggcc gggccggcgc
     3361 cggcgactcc gatcagcgac gccggtcccg ggccggtcga ccgcgcggcc gaccttctcg
     3421 tcgcgctcgc cgcgcgccgc tgggacccgc agggcagggc c
//
LOCUS       DASVQZ010000040         5909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_202300, whole genome shotgun sequence.
ACCESSION   DASVQZ010000040 DASVQZ010000000
VERSION     DASVQZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202300"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VF547_01105"
     CDS             complement(<1..888)
                     /locus_tag="VF547_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8261452.1"
                     /translation="MTAVAPVGANATRLWGLTAEERLRRIARAQGLEVREDGELLVNL
                     GYAFDPSWLKLVAARPGLLVTRGGVPVLAHATTARAEIARGMQEGRKVLAARDILPWE
                     QAGDLENEELRKKERPFMERLEPGTVRSLERASSFGAYKGVTDLLTKYLWPEWALVLT
                     RWAARIGMTPNQVSLVGALLCLLATWLFWRGWYWPGLAAGLVFMVLDTVDGKLARCTI
                     TSSRIGEAIDHGIDLLHPPFWWWAWGVGLHAYGRPLGEDLFLQVMVVLVGGYVVQRLI
                     EGIFMRKYGMHIHVWRRFDS"
     gene            complement(885..2450)
                     /locus_tag="VF547_01110"
     CDS             complement(885..2450)
                     /locus_tag="VF547_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8261453.1"
                     /translation="MIDLNQANGPLDLEGEVCVIGAGAAGISLTRRLLKLGRTVVLLE
                     SGGLDYEAPAADLNAGENAGEDYYDLEDSRLRFFGGTTAIWGGRCAELDPIDLEARDY
                     VPHSGWPLGWDELERWYDEARPLFGLPPRRPSPDDLRRAGVPLPRFEELETPLWTFDR
                     RFNRFAWESCADLEADPRCQVVTHATATSIVADGRGIERIEARSLGQGRLTVRASAFV
                     LAAGGIENPRLLLASGLGNGHDQVGRYFMEHPHARGGRIVEGSAWRLLKAFGRRHRVG
                     GEDLAALIAPSAALQRREGILNTSLTIVARQPQGRRQFIGMKAYSGLKHSLAPTKRGR
                     ALWMVTKKAAGWAQRHVDPARPWLLHKLGNLDVALLVRGEQAPNPESRVTLTGDRDPT
                     GVPRAKLDWRLSGIDVMSVAVLVDALGRELERLGLGRVEPAGWLGSGRWQTDPLVSSH
                     PIGGYHHMGTTRMGLDPRSSVTDSDGRVHGLGNLWVAGSSLFPTGGWANPTLTIVALA
                     LRSAERISAELGR"
     gene            complement(2527..4044)
                     /gene="hutH"
                     /locus_tag="VF547_01115"
     CDS             complement(2527..4044)
                     /gene="hutH"
                     /locus_tag="VF547_01115"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX8261454.1"
                     /translation="MITLRPGSVTLAELRSIWAGAPVELAAEAWPAIDAAAACVGKIL
                     ESGRTVYGVNTGFGLLAQTRIPADRLAELQRNLVMSHSCGLGDDLSRPVVRLAMATKV
                     VGLARGHSGVRREVVERLLALLDADALPCVPSQGSVGASGDLAPLGHLSAAMLGEGRI
                     ELRGTIMPAGEALRALGLEPLVLGPKEGLALINGTQVSTAVALDTLFTAERVFSSALV
                     SGALSLDALKGTDAAFDPRIHEARGQPGQIAVAAALKRLIEGSEIRHSHDDCGRVQDP
                     YSFRCQPQVMGACLDMLGNVSRTLEIEANAVTDNPLLFPESGDSLSGGNFHAEPVAFA
                     ADMLAMAMCEAGSLSERRTAVLVDPKMSGLPPFLVEDSGVNSGFMIAQVTAAALVSEN
                     KSLAWPASVDTVPTSGNQEDHVSMATHAGTKARRIAGNAAGVVGIELLAAAQGCDFHA
                     PLKSSPRLEAARSEIRRNVPHYAADRYLADELEWAQDAVLAGTLSGEVEAELFGR"
     gene            complement(4121..5780)
                     /gene="hutU"
                     /locus_tag="VF547_01120"
                     /pseudo
     CDS             complement(4121..5780)
                     /gene="hutU"
                     /locus_tag="VF547_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
BASE COUNT          878 a         2158 c         2009 g          864 t
ORIGIN      
        1 gctgtcgaac cgccgccaga cgtggatgtg catcccgtac ttacgcatga agatgccttc
       61 gatcagccgc tggaccacat atccgcccac cagcacgacc atcacctgca ggaacaggtc
      121 ttcgccgagc gggcggccat aggcgtggag cccgaccccc caggcccacc accagaaagg
      181 cgggtggagg aggtcgatgc catggtcgat cgcctcgccg atcctcgagg acgtgatggt
      241 gcagcgggcg agcttgccgt cgaccgtgtc gagcaccatg aagacgagcc ccgccgccag
      301 cccgggccag taccagcccc gccagaacag ccaggtcgcg agcaggcaga gcagcgcgcc
      361 gaccagcgac acctggttcg gggtcatgcc aatccgcgcg gcccatcggg tcagcaccag
      421 cgcccattcc ggccacagat atttggtgag caggtcggtg acgcccttgt aggcgccgaa
      481 agagctcgcc cgctccagcg agcggacggt cccgggctcc agccgctcca tgaacggccg
      541 ctccttcttg cgcagctcct cattctcgag gtcgccggcc tgctcccatg gcagtatgtc
      601 cctcgcggca aggaccttgc gcccttcctg cattccgcgc gcgatttccg cccgggcggt
      661 cgtggcatgg gccagcaccg gcactccgcc ccgggttacc agcagccccg gccgggcggc
      721 caccagcttc agccaggagg ggtcgaaggc atagccgagg ttgaccagca gctcgccgtc
      781 ctcgcggacc tccagaccct gcgcccgggc gatgcggcga agccgctcct cggcggtcag
      841 gccccacagc cgagtcgcgt tggcgcccac cggcgcgacc gcggtcaccg tcccagctcc
      901 gccgagatcc gctccgcgct cctcagcgcc agggcgacga tggtcagggt cggattggcc
      961 cagccgccgg ttgggaagag cgacgatccc gcgacccaca gattgccgag cccgtgcacc
     1021 cgcccgtccg aatcggtgac gctgctccgc ggatcgagcc ccatccgcgt cgtccccata
     1081 tggtggtagc cgccgatcgg gtgcgaggag acgagcgggt cggtctgcca gcggccggag
     1141 ccgagccagc ccgccggctc gacacgcccg agcccgagcc gttcgagctc ccggccgagc
     1201 gcgtcgacca gcacggcgac gctcatgacg tcgatcccgc tcagacgcca gtcgagcttc
     1261 gcccgcggca ctccggtcgg gtccctgtcg ccggtcagcg tcacccggct ctccggattg
     1321 ggcgcctgct cgccgcgcac gagcagcgcc acgtcgagat tgccgagctt gtggagcagc
     1381 cagggcctcg ccgggtcgac atggcgctga gcccagccgg ccgccttctt cgtcaccatc
     1441 cacagcgccc gtccgcgctt ggtcggcgcc aggctgtgct tgagcccgga ataggccttc
     1501 atgccgatga actgccgccg tccctggggc tggcgggcga cgatggtcag cgaggtgttg
     1561 aggatgccct cgcgccgctg cagcgcggcg gacggggcga tcaaggcggc gagatcctcg
     1621 ccgcccaccc ggtgccggcg tccgaacgcc ttcaggagcc gccacgccga gccctcgacg
     1681 atgcgcccgc cgcgcgcgtg cggatgctcc atgaaatagc gcccgacctg gtcgtggccg
     1741 ttgccgaggc cggaggcgag cagcagccgc gggttctcga tcccgcccgc cgccagcacg
     1801 aacgccgagg cgcgcaccgt cagccgccct tggccgagcg agcgcgcctc gatccgctcg
     1861 atgccgcggc cgtccgcgac gatcgacgtc gccgtggcat gggtcaccac ctggcatctg
     1921 gggtcggcct cgaggtcggc gcagctctcc caggcgaagc ggttgaaccg ccggtcgaag
     1981 gtccagagcg gagtctcgag ctcttcgaac cggggcaggg gcacgccggc gcgccgcagg
     2041 tcgtcgggcg agggtcgcct gggcggcagc ccgaaaagcg gccgggcctc gtcgtaccag
     2101 cgctccagct cgtcccagcc cagcggccag cccgaatgcg gcacatagtc gcgcgcttcc
     2161 aggtcgatcg ggtcgagctc ggcgcagcgc ccgccccaga tggcggtggt gccgccgaag
     2221 aagcgcagcc gggaatcctc gaggtcgtaa tagtcctcgc cggcattctc gccggcgttg
     2281 aggtcggcgg ccggcgcctc atagtcgagc ccgccgctct ccagcaggac gaccgttcgc
     2341 ccgagcttca gcagccgccg ggtgaggctg atccccgccg cgccggcgcc gatcacgcac
     2401 acctcgcctt cgagatccag cgggccgttc gcctggttga ggtcgatcat tcggtcaccc
     2461 cagccaaggc tggggtcgat gccgagtcgg tatggatgcc agccttcgct ggcatgacgg
     2521 gggggctcac cttccgaaca actccgcctc gacctcgccc gacagcgttc ccgccagcac
     2581 cgcgtcctgc gcccattcga gctcgtcggc cagataccgg tcggccgcat aatgcggcac
     2641 attgcggcgg atttcggacc gcgccgcctc gagccgcggc gagctcttca gcggcgcatg
     2701 gaaatcgcac ccctgcgcgg ccgcgagcag ctcgatcccg accacgccgg ccgcattgcc
     2761 ggcgatccgc cgggccttgg tgccggcatg ggtcgccatc gagacatggt cctcctggtt
     2821 gcccgacgtc ggcacggtgt cgacgctggc gggccaggcc aggctcttgt tctccgacac
     2881 cagggccgcc gccgtcacct gggcgatcat gaagccgctg ttgacgccgc tgtcctcgac
     2941 caggaacggg ggcaggccgc tcatcttggg gtcgaccagc accgccgtcc ggcgctccga
     3001 gaggctgccg gcctcgcaca tcgccatcgc cagcatgtcg gcggcgaacg ccaccggttc
     3061 ggcgtgaaag ttgccgcccg acagcgaatc gccgctctcc gggaacagca acggattgtc
     3121 ggtgacggca ttcgcctcga tttcgagcgt gcgggagaca tttccaagca tatccaggca
     3181 agcgcccatc acctgcggct ggcagcggaa gctgtagggg tcctgcaccc gcccgcaatc
     3241 gtcgtgcgag tggcggatct cgctgccctc gatcagccgc ttcagcgccg ccgcgacggc
     3301 gatctggccg ggctggcccc gggcttcgtg gatcctcggg tcgaaggcgg cgtcggtgcc
     3361 cttgagcgcg tcgagcgaca gcgctccgga gaccagcgcg gacgagaaca cccgctccgc
     3421 cgtgaacagg gtgtcgagcg cgaccgcggt cgacacctgg gtcccgttga tcagcgccag
     3481 cccctccttg gggcccagca ccagcggctc cagccccagc gcgcgcagcg cctcgccggc
     3541 cggcatgatc gtgcccctga gctcgatccg cccctcgccg agcatcgcgg cgctgagatg
     3601 gccgagcggc gcgaggtcgc cggaggcgcc gaccgacccc tggctcggga cgcagggcaa
     3661 ggcatcggca tcgagcaagg cgagcagccg ctcgaccacc tcgcgccgga cccccgaatg
     3721 gccgcgggcg aggccgacca ccttggtcgc catggcgagc cgaacgacgg gccggctgag
     3781 gtcgtcgccg agcccgcagc tgtgcgacat caccaggttg cgctggagct cggccagccg
     3841 gtcggcgggg atccgggtct gggcgagcag cccgaagccg gtattgaccc cgtagacggt
     3901 gcggccgctc tcgaggattt tcccgacgca ggcggcggcg gcgtcgatgg cgggccaggc
     3961 ctcggcggcg agctccaccg gagcgccggc ccagatcgag cgaagctcgg cgagggtgac
     4021 ggagccggga cggagggtga tcatgcgtca ccccagcgaa ggctggggtc catgccgatt
     4081 ctgaatggat gccagccttc gctggcatga cgggggaggt tcatggcccc accatcggca
     4141 ggtcgagccc ctgctcgcgg gcgcagtcaa gcgcttcctc atagcccgcg tcggcatggc
     4201 gcatcacgcc ggtgccgggg tcgttccaca gcacccgctc gagccgccgc gccgcttccg
     4261 gcgtgccgtc ggcgacgatg accattccgg cgtgctgcga atagcccatg ccgacgccgc
     4321 cgccgtggtg gagcgagacc caggtcgcgc cggaggcggt gttgagaagg gcgttgagca
     4381 ggggccagtc cgataccgcg tccgacccgt ccttcatcgc ttcggtctcg cggttggggc
     4441 tcgccaccga gccggaatcg agatggtcgc ggccgatcac caccggcgcc ttcagttcgc
     4501 ccgaggcgac catctcgttg aacgccatgc ccagccggtg cctctgcccc agcccgaccc
     4561 agcagatccg cgccggaagg ccctggaagg cgatccgctc gcgcgccatg tcgagccagt
     4621 tgtggagatg cggatcgtcc gggatcagcg ccttgacccg ctggtcggtc cggtagatgt
     4681 cctccggatc cccggagagc gccgcccacc ggaacgggcc gatgccccgg cagaagagcg
     4741 gccggacgta ggccgggacg aagcccggaa aatcgaacgc gcgggtcacg ccttggtcct
     4801 tcgcgatctg gcggatattg ttgccataat cgaaggtcgg gacgcccatc gcgcggaagg
     4861 cgagcatcgc ctcgacatgc gccgccatgg attctcttgc agccttctcc acggcggcgg
     4921 ggtcgcgctc gcgcatgttc atccatttct cgacggtcca gccggccggc agatagccgt
     4981 tcaccgggtc gtgcgccgac gtctggtcgg tcaccgcgtc cggccggatc cccctccgca
     5041 ccatctccgg cagcacctcc gcggcattgc cgagaagccc cacggaagtg ggtgcaatgg
     5101 ccgccccgat gatctccagc gcctcgtcca gcgattccgc ccgacggtcg agatagcggg
     5161 tctcgagccg cttcgcgatc cggctctcct ggcattcgat ggccaggcag tgcgccccgg
     5221 ccatcaccgc cgccagcggc tgcgcgccgc ccatcccgcc caggcccgcg gtgaggatcc
     5281 agcggccctt cagatcgccg ccataatgct ggcggcccat ctccgcgaag gtctcgtagg
     5341 tgccctggac gatgccctgg gtgccgatgt agatccacga tccggccgtc atctggccgt
     5401 acatcatcag gcccttgcga tcgagctcgt ggaaaatgct cccaggtcgc ccatttcggc
     5461 accaggttcg aattggcgat gaggacgcgc ggcgcgtcgg cgtgggtgcg gaagacgccg
     5521 acgggcttgc cggactggac gaggagggtc tcgtcctccg cgagccgttc gagcgtctcg
     5581 acgatgcggt cgaagctctc ccagtcccgc gccgcgcggc cgatgccgcc gtagacgaca
     5641 agctcctgcg gcttctcggc gacctcggga tcgaggttgt tcatcagcat gcgcagcggc
     5701 gcttcggtct gccagctctt ggcggaaatc tcggggcctc gcggcgcccg gattagcggg
     5761 gcgttggcga tcaggttcat cggccctctc agggttcgac agtgggaaac agatccagcc
     5821 cagccgcctg caccacggcc cccgaacgga cgagatcggc ggccgctgcc atgtcgtcgg
     5881 ccaagtagcg gtccgcttcg aggcggggc
//
LOCUS       DASVQZ010000041         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_202631, whole genome shotgun sequence.
ACCESSION   DASVQZ010000041 DASVQZ010000000
VERSION     DASVQZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202631"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VF547_01125"
     CDS             <1..810
                     /locus_tag="VF547_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX8261455.1"
                     /translation="LGAATASTQVARLAVREVNLPPDERPEPKRLTPWKLDLQVDAPG
                     RLMVTGLGINSEWSADLAIAGTVTEPRIKGQADLVRGSYDFAGRRFDLERGAIRFLGE
                     SPVNPALDITAEGGVQGLNAVIRVTGRGMKPEITFTSTPALPQDELLSRLLFGTSITN
                     LSAPEALQLAAAVASLNSSGGNLDPINAVRAATGLDRLRIVPADITTGQGTAIAAGKY
                     LGRRVYVEVVTDARGYSATTVEYRITRWLALLSSISTIGRNSVNVRVSKDY"
     gene            886..1323
                     /locus_tag="VF547_01130"
     CDS             886..1323
                     /locus_tag="VF547_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261456.1"
                     /translation="MGSKPIRIEDQGRRAERLALDEAARLHPNSWSSLEIRMIDLSEF
                     GFRAACDARLRPGGGISLDIPGLGPIDAQVEWHRGGEFGARFFAAIDLDLCLWPLDGQ
                     HRHLAQMLVERAAANQAGRDGADRQLRRQILSALPIRKGSFSA"
     gene            1453..>2469
                     /locus_tag="VF547_01135"
     CDS             1453..>2469
                     /locus_tag="VF547_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8261457.1"
                     /translation="MARFSGLGEAKAGDQGVPSTLALLAFDFGAPASDIAGAIAADRA
                     RAVAAAVPLLAGSHLLWACIVAWRLLASDSPFASPAFLLLAALLAVDALGWALAVRAR
                     AAPARLVQLVAAQVLLTGGLWAAAAAFTAGIPGGASPAVVAALLCGAGSGLALFFPVP
                     ALAAASCALVWATAAAVGLDPELLGIGAALVLILCLVSLFRARDAVLGAERRLSAEAE
                     AETAGRFVEEYEASGRGWFWETNSEGAVTYASEQLAARLGLTRGAMIGRRFEDLLFAE
                     EAGNPLGFHLSARFPFTEVIVRAPGAQETWWALSGSPNFDRYGRFLGFRGLGVNLSEQ
                     QRGEA"
BASE COUNT          317 a          938 c          817 g          397 t
ORIGIN      
        1 ctcggcgccg caaccgcctc cacccaggtg gcgcggctcg ccgtgcgcga ggtcaacctt
       61 ccgcccgacg agcggcccga gcccaagcgc ctcaccccct ggaagctgga cctgcaggtc
      121 gacgcaccgg gccggctgat ggtgaccggc ctcggcatca attcggaatg gtcggccgac
      181 ctcgccatcg ccggcaccgt caccgagccc cggatcaagg gccaggccga cctggtgcgc
      241 ggcagctacg atttcgccgg ccgccgcttc gacctcgagc gcggcgcgat ccgcttcctc
      301 ggcgaaagcc cggtcaatcc ggcgctggac atcaccgcgg agggcggcgt ccagggcctc
      361 aacgccgtca tccgcgtcac cgggcgcggc atgaagccgg agatcacctt cacctcgacc
      421 cccgctctgc cgcaggacga gctgctgagc cggctgctgt tcgggacctc gatcaccaat
      481 ctctcggcac ccgaggcgct gcagctcgcc gcggcggtcg cctcgctcaa ttcgagcggc
      541 ggcaatctcg atccgatcaa tgcggtgcgc gccgccaccg gcctcgaccg cctccgcatc
      601 gttccggccg acatcaccac cggccaaggc acagccatcg ccgccggcaa gtatctcggc
      661 cgccgcgtct acgtcgaagt ggtcaccgac gcgcgcggct attccgcgac caccgtcgaa
      721 taccgcatca cccgctggct cgccctcctc tccagcatct ccacgatcgg ccgcaacagc
      781 gtcaacgtga gggtttcgaa ggattattga gcgggagaag tctcgtcagc cattcgttaa
      841 ccccgctccg ctaccttcac aggccgcaca catggggagc gggttttggg atcgaagccg
      901 atccggatcg aagaccaggg gcgcagggcc gagcggctgg cgctggacga ggccgcgcgc
      961 ctgcatccga acagctggtc cagcctcgaa atccgcatga tcgacctgtc cgaattcggc
     1021 ttccgcgccg cctgcgacgc gcggctccgg ccgggcggcg gcatctcgct cgatattccc
     1081 ggcctcgggc ccatcgacgc gcaggtcgag tggcatcgcg gcggcgagtt cggcgcgcgc
     1141 ttcttcgcag ccatcgacct tgacctctgc ctctggccgc tcgacgggca gcaccgccac
     1201 ctcgcgcaga tgctggtcga acgggccgcg gccaaccagg ccggccgcga tggcgccgac
     1261 cgacagcttc gccggcagat cctgtccgca ttgccgatcc gcaagggctc cttctccgct
     1321 taagcaggct ttttgcgggc gcgttaaccc tccggcgcgg cactttctta acctagatca
     1381 tgcgaaagtc gcgccgcgcc cgcgcgcgtc gtgcggcgga gatgacaggg agcgggacaa
     1441 aggcggatgc gcatggcgcg cttttcaggt ttgggcgagg cgaaagcggg tgaccagggc
     1501 gtgccctcga cgctcgccct gctcgccttc gacttcggcg cgccggccag cgacatcgcc
     1561 ggggcgatcg ccgcggaccg cgcccgggcc gtggccgcgg ccgtgccgct actcgccggc
     1621 tcgcacctgc tctgggcctg catcgtcgcc tggcgtctcc tcgcctcgga ttcccccttc
     1681 gcttccccgg ccttccttct gctggcggcc ctgcttgccg tggacgccct gggatgggcg
     1741 ctggccgttc gcgcccgcgc cgcgccggcg cgtctcgttc aattggtcgc cgcgcaggtc
     1801 ctgctcaccg gcgggctctg ggccgccgcc gccgccttca ccgccggaat cccgggcggc
     1861 gcatcgccgg ccgtcgtcgc cgccttgctg tgcggtgccg gctcgggtct cgcgctcttc
     1921 ttccccgtcc ccgcgttggc ggccgcgagc tgcgccctcg tctgggcgac cgcggcggcg
     1981 gtcggcctcg acccggagct gctcggcatc ggcgccgcgc tcgtcctcat cctgtgcctc
     2041 gtcagcctgt tccgcgcgcg cgatgcggta ctgggcgcgg aacggcgcct gtcggccgaa
     2101 gcggaggccg agacggcagg gcgattcgtg gaggaatatg aggcttccgg ccgcggctgg
     2161 ttctgggaga cgaattccga gggcgctgtg acctacgcct ccgagcagct cgcggcccgg
     2221 ctcggcctca cccgcggcgc gatgatcggc cgccgcttcg aggacctgct gttcgccgag
     2281 gaggccggca acccgctcgg cttccatctc tcggcgcgct tccccttcac cgaggtaatc
     2341 gtgcgcgcgc ccggcgcgca ggagacctgg tgggcgctgt ccggcagccc caatttcgat
     2401 cgctacgggc gcttcctcgg ttttcgcggg ctcggcgtga atctctccga gcagcagcgc
     2461 ggcgaagcg
//
LOCUS       DASVQZ010000042         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_202632, whole genome shotgun sequence.
ACCESSION   DASVQZ010000042 DASVQZ010000000
VERSION     DASVQZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202632"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..>1559)
                     /locus_tag="VF547_01140"
     CDS             complement(113..>1559)
                     /locus_tag="VF547_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX8261458.1"
                     /translation="RTRLAGTRQRLADHIRERLPGGAGGIAAALATGDQGGIPEEDAE
                     AMRRSGLAHLLSVSGLHLTAVVGAVMLLTLKLLALSPFLALRFRLVLVAAGTGALAGV
                     AYTLLTGAEVPTVRSCIAALLVLLGIALGREALTLRLVAVGALIVLLLWPESLPGASF
                     QMSFAAITAIVALHEHPRLRALLARREELWPMKLGRFLLGLVLTGFAVELALVPIALW
                     HFHQAGLYGALANIVAIPLTTFVIMPVEALALLFDLAGLGAPFWWLAGAALDLLLGLA
                     HAVAAAPGAVARLPTLPPSAFALMVAGGLWLALWRTGWRRWGLLPASAGAAAALLAPA
                     PDLLVTGDGRHLALRSPSGELALLRGRAGDYVRDLMSQSAGLEGEAGELETLGSAECS
                     DDFCAADLRGWRLLASRSDRLVPVPALAKACAEADIVVSDRRLPAACRPRWLKADRDF
                     LAKSGGLAITLGQTPKVTTVSSGDRHPWRLN"
BASE COUNT          254 a          537 c          600 g          168 t
ORIGIN      
        1 agcggctgtc gtcaccccgc gaaggcgggg tccctcttct aaccgggttc gacaagagaa
       61 ggagaagcgg attcccgctt tcgcgggaat gacgggaggg ggcagatccc acctaattga
      121 gccgccacgg gtgccggtcg ccgctcgata cggtcgtcac cttcggggtc tggcccagcg
      181 tgatcgccag gcctccgctc ttcgccagga aatcgcggtc ggccttgagc cagcgcgggc
      241 ggcaggcggc ggggagccga cggtcggaga cgacgatgtc ggcctcggcg caggccttcg
      301 ccaaggccgg caccggcacc agccggtcgc tccggctcgc cagcagccgc cagccgcgaa
      361 gatcggcggc gcagaaatcg tcgctgcatt cggcggagcc aagagtctcc agctcgcccg
      421 cctcgccctc caggccggcg ctctggctca tcaggtcgcg gacatagtcg cccgcccggc
      481 ctcggagcag ggccagctcg ccgctcgggc tgcgaagggc aaggtggcgg ccgtcgccgg
      541 tgacgagcag gtccggggcg ggcgcgagca aggccgccgc cgctccagcc gaggccggta
      601 ggaggcccca gcggcgccag ccggtccgcc acaaggcgag ccacaggccg ccggccacca
      661 tcaaagcgaa ggccgacggc ggaagggtcg gaaggcgggc gacggctccg ggcgcggcgg
      721 cgacggcgtg ggcgaggccg aggaggaggt cgagcgccgc tccggcgagc caccagaagg
      781 gcgccccgag cccggccagg tcgaacagca aggcgagcgc ctcgaccggc atgatgacga
      841 aggtggtgag cgggatcgcg acgatgttgg cgagcgcgcc gtagaggccg gcctggtgga
      901 aatgccacag ggcgatcggg acgagcgcga gctcgaccgc gaatccggtg aggacgaggc
      961 cgagcaggaa gcggccgagc ttcatcggcc agagctcctc gcgccgggcc agcagcgcgc
     1021 ggagcctcgg atgctcgtga agggcgacga tggcggtgat cgccgcgaag ctcatctgga
     1081 agctcgcccc gggcagcgat tccggccaga gcaggagcac gatcagggcc ccgaccgcca
     1141 ccagcctgag ggtcagcgct tcccgcccaa gcgcgatccc gagcaggacg agcaaggcgg
     1201 cgatgcagga tcggacggtc ggcacctccg cgccggtgag cagggtgtag gcgacccccg
     1261 ccagcgcccc tgtcccggcc gccaccagga cgagccggaa gcgcaaggcg aggaacgggc
     1321 tgagcgcgag cagcttcagg gtcaggagca tcaccgctcc gacgacggcg gtgaggtgga
     1381 ggccgctcac cgagagcagg tgggcgaggc ccgagcggcg catcgcctcg gcatcctcct
     1441 cggggattcc gccctggtct ccggtcgcca gagccgcggc gatccctccg gcgcctccgg
     1501 gcagccgttc gcgtatatgg tcggcgaggc gctggcgggt gccggccagc cgggtccgc
//
LOCUS       DASVQZ010000043         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_298381, whole genome shotgun sequence.
ACCESSION   DASVQZ010000043 DASVQZ010000000
VERSION     DASVQZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298381"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VF547_01145"
     CDS             complement(<1..1232)
                     /locus_tag="VF547_01145"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8261459.1"
                     /translation="MLLLRYCLLALLLLVAAPLQAAGDSSWFYRNSDIAPDPAWTFGT
                     LPNGLRYAVRRNALPEGQVSVRLRIDAGSLHEADEERGWAHFVEHMAFRGTKNFGDGE
                     VRETWQRLGASFGSDTNASTEPTQTVYMLDLPRADRDSLDTSLRLLADMADTAAFDPK
                     ILDMERGVVLSEYGRRTELAVKLRELMMPLFFGGLKFAERDTIGTEATLKGATAEGLK
                     AFYERWYRPDRATLVMVGDADPKLMEELIARRFGGWTASGPAPAEPDYGRIADVAEPT
                     ASLAYPGSPHFATLSWIRPYTPVPPTKARERRDLARTLARRILNRRLEAKARGDSAFL
                     NAQVQAEQSVNVADYTQMSVMAKEGRWQEALKEAYAILADALKAPPSEAEIGRELENI
                     RTAGRAALQGDPTQRSQQR"
     gene            1453..2232
                     /locus_tag="VF547_01150"
     CDS             1453..2232
                     /locus_tag="VF547_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ImuA"
                     /protein_id="HEX8261460.1"
                     /translation="MNTRVVIREMYRNLARAERLAALRAEVRALESTGAAEGRECLPF
                     GIDAIDSRLAAGGLPVAALHEAAGRRPAPGDEAAATLFLASIAARRPGTVLWALARRD
                     LFAPGLAGAGLGPERILYAECGRDEDVLAVMEEGLRHGGLAAVVGEIGRAVMPATRRL
                     QLAAEEGGTLALMLRRWRGTGEDPLGPPSAAVTRWHLACAPSAPLPVPGIGRPRWRLA
                     LARQRGGAPFDLIVETPDAEARLALPAEFEHRPAQGERRAA"
     gene            2531..>3277
                     /locus_tag="VF547_01155"
     CDS             2531..>3277
                     /locus_tag="VF547_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX8261461.1"
                     /translation="MNFEPEGKPPLLLSRRVGNRLEVAAASPEARALGLHPGMAVAQA
                     RALVPGLDIRDHDPEADSAVLQRLALFAARRWTPRAAVSPPDGLFLDLSGVAHLFGGE
                     EAMCRRILAFCGRAGFAARIAVAGTAGAAHALARFGPGGLCPAGGEAEALAPLPLAAL
                     RVDESALAAARRLGLETVGDLLPLPRAPLQRRFGKTLLARLDQALGRSGEPIEPVVPE
                     EPPCAVLRFAEPIATAEAIEKAVEDLLGELV"
BASE COUNT          400 a         1243 c         1167 g          467 t
ORIGIN      
        1 cgctgctgcg agcgctgggt cgggtcgcct tgaagcgcgg cccggccggc ggtccggata
       61 ttctcgagct cgcggccgat ctcggcctcg ctcggcggcg ccttcagggc atcggcgagg
      121 atcgcatagg cctccttgag cgcctcctgc cagcgcccct ccttcgccat caccgacatc
      181 tgggtgtaat cggcgacgtt gacggactgc tcggcctgga cctgggcgtt gaggaaggcg
      241 gaatcgccgc gcgccttcgc ctcgaggcgg cggttgagga tgcgccgcgc gagcgtccgg
      301 gcgaggtcgc gccgctcgcg cgccttggtc ggcgggacgg gcgtgtaggg ccggatccag
      361 ctcagggtcg cgaaatgcgg cgagcccgga taggcgagcg aagcggtcgg ctcggcgacg
      421 tcggcgatgc ggccgtaatc gggctcggcg ggggccgggc cgctcgccgt ccagccgccg
      481 aagcgccggg cgatcagctc ctccatcagc ttcgggtcgg catcgccgac catcaccagc
      541 gtcgcccgat cgggccggta ccagcgctcg tagaaggcct tgaggccctc ggcggtcgcg
      601 cccttcaggg tcgcctcggt gccgatcgtg tcccgctccg cgaatttgag gccgccgaag
      661 aagagcggca tcatcagctc gcgcagcttc accgcaagct cggtccgccg cccatattcg
      721 gagaggacga ctccgcgctc catgtcgagg atcttcgggt cgaaggccgc cgtgtcggcc
      781 atgtcggcga gcaggcgcag gctggtgtcg agcgagtccc ggtcggcgcg gggcaggtcg
      841 agcatgtaga cggtctgggt cggctcggtc gacgcgttgg tgtcgctgcc gaagctcgcg
      901 ccgagccgct gccaggtctc gcggacctcg ccgtcgccga aattcttggt cccccggaac
      961 gccatatgct cgacgaaatg cgcccagccg cgctcctcgt cggcttcgtg cagcgagccg
     1021 gcgtcgatcc tcaggcgaac cgacacctgt ccttccggca gcgcgttgcg gcggacggcg
     1081 tagcgcagcc cgttgggcag ggtgccgaac gtccaggccg ggtcgggcgc gatgtcgctg
     1141 ttccggtaga accagctcga atcgccggcg gcctggagcg gcgcggcgac gaggaggagg
     1201 agagcgagga ggcagtagcg gaggaggagc atggggttcc gatagtcgag aagggagggt
     1261 ggccttagcc gttcctcccc ggaacgggga gggggaccat gcgaagcatg gtggaggggg
     1321 cccgcgtctc cgcctgatcc ggcgccgggc cccctccacc acgcgcttcg cgcgcggtcc
     1381 ccctccccgt tccggggagg agcggtttcc cgttcgccaa cttgactctc gctgaacagg
     1441 ctggaacatt ctgtgaacac gagagtcgtg attcgcgaaa tgtaccgtaa cctcgcccga
     1501 gccgaacgcc tggccgccct gcgcgcggag gtgcgggcgc tcgaatcgac gggcgccgcg
     1561 gagggacgcg aatgccttcc cttcgggatc gacgccatcg attcgcggct cgcggccggc
     1621 gggctgccgg tcgcggccct ccatgaagcg gcgggccggc gcccggcgcc cggcgacgag
     1681 gccgccgcaa ccctgttcct cgcctcgatc gcggcccgcc gccccggcac cgtgctgtgg
     1741 gccctcgccc gccgcgacct tttcgctccc ggcctggctg gcgctgggct cgggccggag
     1801 cggatcctct atgccgagtg cgggcgcgac gaggacgtgc tggcggtgat ggaggagggg
     1861 cttcgccacg gcggcctcgc cgcggtggtc ggcgagatcg gacgggcggt catgcccgcc
     1921 acccgccggc tccagctcgc cgccgaggaa ggcggaacgc tcgccctcat gcttcggcgc
     1981 tggcgcggca ccggcgagga cccgctcggc cccccgtccg ccgccgtcac ccgctggcac
     2041 ctcgcctgcg ccccttccgc ccccttgccc gtccccggca tcggccgccc ccgctggcgc
     2101 ctcgccctcg cccgccagcg cggcggcgcc ccgttcgacc tgatagtgga gacccccgat
     2161 gccgaggctc gcctcgctct acctgccgaa tttgagcacc gaccggctca gggcgagcga
     2221 agggcggcgt gaggcgatga attcctcccc aggaaatcct ggggaggaat tagaacgcga
     2281 accccgctcc tccccggaac ggggaggggg accgccggcg aagccggggg tggagggggc
     2341 ccagcgtctg ggcaatggag gcgtgggccc cctccaccag ccttcggctg gtccgcctcc
     2401 ccgtcccggg gaggagcgag gcagcgaacc ctgctcctgc ccgaacgaag cgcactggcg
     2461 ccccggcgct cactgggccc gcgccggcgc tgcgaagttc acgaaaagga cctccgactc
     2521 ggtgaacttc atgaacttcg agccggaggg gaagcctccc ctcctcctct cccgccgggt
     2581 cggcaaccgg ctcgaggtcg ccgccgcctc gcccgaagcg cgcgccctcg gcctccaccc
     2641 cggcatggcc gtcgcccagg cgcgcgccct cgtccccggc ctcgacatcc gcgatcacga
     2701 tcccgaggcg gactcggccg tcctccagcg tctcgccctc ttcgccgccc gccgctggac
     2761 cccgcgcgcc gccgtctcgc cccccgacgg cctgttcctc gacctcagcg gagtggccca
     2821 tctgttcggc ggcgaggagg ccatgtgccg ccggatcctc gccttctgcg ggcgcgccgg
     2881 cttcgccgcc cggatcgcgg tcgccggcac cgcgggcgcc gcccacgccc tggcccgctt
     2941 cggccccggc ggcctgtgcc ccgccggcgg cgaggccgag gcgctcgccc cgctcccgct
     3001 ggccgccttg cgggtcgacg aatcggccct cgccgccgcc cgccggctcg gcctcgagac
     3061 ggtcggcgac ctgctgccgc tgccccgcgc gcccctccag cgccgcttcg gcaagaccct
     3121 gctcgcccgc ctcgaccagg cgctcggccg ctccggcgag ccgatcgagc cggtcgtgcc
     3181 cgaggagccg ccttgcgccg tccttcgctt cgccgagccg atcgccaccg ccgaggcgat
     3241 cgaaaaggcc gttgaggatc tgctcggcga gctggtc
//
LOCUS       DASVQZ010000044         5639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_683506, whole genome shotgun sequence.
ACCESSION   DASVQZ010000044 DASVQZ010000000
VERSION     DASVQZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683506"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VF547_01160"
     CDS             complement(<1..872)
                     /locus_tag="VF547_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261462.1"
                     /translation="MSRSPPLRFLVLLLAGWCGMRTAVLAPGWWTPPASAGRRAPAER
                     RPALAPRPAAPASSERDAAPGRVAAAAALPRRRPTPKPAAVPAPVARRTTAVAELAWD
                     LAPASPSGRRGPTGALPGEAVPARAAAASRWSFAAWSYLREGNSAPLAAGGLLGGSQA
                     GLRLGYRLNRDEARPLALTARLSTPLRRPAAEASLGLDWQPLRRLPVHLLGERRQRLG
                     GEGRSAFALTVHGGLSDAPLGRLRIDAYAQAGIVGLRSADRFGDGALRLSLPLGGRVR
                     AGLGAWAAAQPGAAR"
     gene            complement(869..2281)
                     /locus_tag="VF547_01165"
     CDS             complement(869..2281)
                     /locus_tag="VF547_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEX8261463.1"
                     /translation="MTPIDFRGAESMGAVGLVFGSLDFIMREAALFAATGFLILGLGD
                     LAVDLLWIGSRLRAPGGRSPSVDELPQPERPGMLAVFVPAWDEAAVIGQMLGAAVSAW
                     GGGDWRLYVGTYPNDPATRAAAAAVAEADPRVRVVVGVDPGPTTKADCLNRLWQALLD
                     DEAEAGRPAKAVILHDAEDVVHPDELRLFDALVERFDLVQLPVLPLIDPYSRWLGGHY
                     ADEFSESHGKELVVRGALGAGLPSAGVGCAFSREALGRLARERGGLPFDADSLTEDYE
                     LGLRIAEAGGRSIFVRAAGRGGGLVATREYFPGTLRAAVAQKARWTAGIALAGWDRLG
                     WRGGLAERWMRLRDRQAPLAALLLFAGYLALLLWLALKVREGTTGAAPAPLPPALSFL
                     VAANMLLLGWRLAMRAAFTAASYGWREGLRAVPRTAIGNAVAMLAAAAALGRYHALRR
                     GGRPRWGKTAHIFPAVPPAR"
     gene            2546..3622
                     /locus_tag="VF547_01170"
     CDS             2546..3622
                     /locus_tag="VF547_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8261464.1"
                     /translation="MAAQPSDPPAPAAIAGPPAAGTYVVHAIDVGTGLSIFVEGHDFT
                     LLYDAGSNDDSARGKGKDRVLAYLAKVRPDMTVLDHLVLSHPHKDHVEMMDDVLEFYQ
                     VRNVWDSGALNDTCGYRALLEAIIAEPGVVYHNSLGSGGTHDVAVKDAGTCHGRKRPA
                     TTLRVPRSSQITNAPVPLGAGATMTILLADGSVKMPRGNFNEASVVARLLLGTRSVLL
                     PGDGEAGGRKKPSDLPKANSAEAKLLACCALQLRSDILVASHHGSMTSSRNAFLDAVG
                     ASQYVVSVGPKAYSGTVLPDSEVIDEYNKRGKLWTTALNDATCPTSRKIGPDKDGRPG
                     GCDNVRIVIGPDGSIAPDYFRAVD"
     gene            3765..4613
                     /locus_tag="VF547_01175"
     CDS             3765..4613
                     /locus_tag="VF547_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX8261465.1"
                     /translation="MYVLAPSPARAWGESGHYTVCEIAYLNLTPRARAEVDRLIAAGG
                     GYDSFTRSCTFPDKPRQRASEHFANYPRNAARVAGPACPGGRPCIVQAIESDLAVLRS
                     PTASDDDKSKALKFLGHWYGDIHQPLHISFADDQGGNLIDQSGSCQFSLHSVWDTCIV
                     ERRLFGPGDDRYARARAAAAALNRTITARQRRQWARTRPWQWAGESYEISRRAATGYC
                     LRKARTCRYSATAAAYVEGAAKRVQTVDDAYLDRARRIVADRLKRGGIRLAHALNRAF
                     DPAYRG"
     gene            4623..4823
                     /locus_tag="VF547_01180"
     CDS             4623..4823
                     /locus_tag="VF547_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261466.1"
                     /translation="MRALSFLAAAASLALGPASLDPCPSGRAAARAYACCKICSTGKA
                     CGDSCIARWKTCHKGKGCACDG"
     gene            4873..>5639
                     /gene="nhaA"
                     /locus_tag="VF547_01185"
     CDS             4873..>5639
                     /gene="nhaA"
                     /locus_tag="VF547_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX8261467.1"
                     /translation="MADARPQRPRSALRAFLKSEAAGGVLLMAAAAAALVVANSPLAS
                     DYQELLHQRVGPMSVHHWINDALMALFFLLVGLEIKREYLDGRLATWERRRLPILAAA
                     AGMAVPALLYLAVAGREPGLAAGWAIPAATDIAFAIGVLALLGKRAPTSLKLFLTTVA
                     IADDLGAVAIIALAYTDSLDAAALGAAAAILLVLYAMGKSGVRRLWPYLIGAGLLWYA
                     VLLSGVHATVAGVLAAAAIPIVAAPGAPDSADSPLHRL"
BASE COUNT          825 a         2099 c         1975 g          740 t
ORIGIN      
        1 cgggccgcgc cgggctgggc ggcggcccag gctccgagtc cggcgcggac gcgtccgcca
       61 agcggcagcg acagccgcag cgccccgtcg ccgaaccggt ccgccgagcg caggccgacg
      121 atccccgcct gggcataggc atcgatccgc aggcggccga gcggcgcgtc gctgaggcct
      181 ccgtggacgg tcagcgcgaa agcggatcgt ccctcgccgc ccagccgctg ccggcgctcc
      241 ccgagcagat ggaccgggag ccggcgcagc ggctgccagt cgaggcccag cgacgcttcg
      301 gcggcggggc gccgcagcgg cgtggacagg cgcgcggtca gggcgagcgg acgggcctcg
      361 tcgcggttca ggcggtagcc gaggcgcagg ccggcctggc tgccgccgag caggccgccg
      421 gcggcgagcg gcgccgaatt cccctcccgc aggtaggacc aggccgcgaa cgaccatcgc
      481 gacgccgcgg cggcccgggc cgggacggcc tcgccgggga gcgcgcctgt gggcccccgc
      541 cggccgctcg gcgaggccgg ggcgaggtcc caggccagtt cggcaacggc cgtcgttcta
      601 cgcgcgacgg gcgccggcac cgccgcaggc ttcggcgtcg ggcgccggcg tgggagtgcg
      661 gcagccgcgg ccacgcggcc gggcgctgcg tcccgctcgg acgaagccgg cgcggcgggg
      721 cgcggcgcca aggcgggccg gcgctcggcc ggcgcccggc ggccggcgct ggcgggcggc
      781 gtccaccatc ccggcgccag cacggccgtc cgcattccgc accagccggc gagcaggagg
      841 acgaggaagc gcagcggcgg cgaccggctc atcgcgccgg cggcaccgcc gggaagatgt
      901 gcgcggtctt gccccagcgc gggcggccgc cgcgccgaag cgcatggtag cgtccaaggg
      961 ccgccgcggc ggcgagcatc gcgacggcgt tgccgatcgc ggtccgaggc acggccctca
     1021 gcccctcgcg ccagccgtag gacgcggcgg tgaatgcggc gcgcatcgcc aggcgccagc
     1081 cgagcaggag catgttcgcc gcgacgagga aggagagcgc cggcggcagc ggcgcgggcg
     1141 cggcgccggt ggtcccctcc cgcaccttga gcgccagcca caggagcagc gcgagatagc
     1201 cggcgaagag cagcagcgcc gcgagcgggg cctggcggtc gcgaagccgc atccagcgct
     1261 cggccaagcc tccgcgccag cccagccggt cccagccggc cagggcgatc ccggccgtcc
     1321 accgcgcctt ctgcgccacc gcggcgcgca gcgtgccggg aaaatattcg cgggtcgcga
     1381 ccagcccgcc gccgcgcccg gcggcgcgga cgaagatcga gcggccgccg gcctcggcga
     1441 tccgcaggcc gagctcgtaa tcctcggtga ggctgtcggc gtcgaagggc aggccgcccc
     1501 gttcccgggc cagccgcccg agcgcctcac gcgaaaaggc gcacccgacg ccggccgagg
     1561 ggaggccggc gccgagcgcg ccacgcacga ccagctcctt gccgtgcgat tcggaaaact
     1621 cgtcggcata atggccgccg agccatcgcg aataaggatc gatcaggggc aggacgggga
     1681 gctggacgag gtcgaaccgc tcgaccagcg cgtcgaacag tcgaagctcg tccggatgga
     1741 cgacgtcctc cgcgtcgtgg aggatgaccg ccttggccgg ccgccccgcc tctgcctcgt
     1801 cgtcgagcag ggcctgccac agccggttga ggcagtcggc cttggtcgtc gggccgggat
     1861 cgacgccgac caccacccga acccgcggat cagcttcggc caccgcggcg gccgcggccc
     1921 gggtcgccgg atcatttgga taagtgccca catagagccg ccagtcgccc ccgccccagg
     1981 ccgagacggc ggcgcccagc atctgcccga tcaccgccgc ctcgtcccag gccggcacga
     2041 agacggcgag catccccggc cgctccggct gcggcagctc gtcgacggag gggctgcggc
     2101 cgccgggcgc gcggagcctc gatccgatcc agagcaggtc gaccgcgagg tcgccgaggc
     2161 ccaggatcag gaacccggtc gccgcgaaca gcgccgcctc gcgcatgatg aagtcaaggc
     2221 tgccgaacac cagccccacg gcgcccatcg attccgcccc cctgaagtcg atgggcgtca
     2281 tcattcattt tcgtccaata aaagcaagtc aattttgaaa acaattgatt ttcggttgcg
     2341 gggcccgccg tggcgagcgg ccggtcgcgg aacttgctcc attcccgagc gaatatcgga
     2401 ataacacata caggtatcgc gatttgcgat atggtccccc gaggctcggc gctcggggcg
     2461 tcagcgagcc ggctgggggg ccgccatgcg caaattgacg atcggttttt tcgctgccgg
     2521 actcgcactg gcctccgggg ccggcatggc ggcgcaaccc tccgatcccc cggccccggc
     2581 ggccatcgcc ggaccgccgg cggcgggcac ttacgtcgtc cacgcgatcg acgtgggcac
     2641 cggcctctcg atcttcgtcg aaggccatga tttcaccctg ctctacgacg ccggcagcaa
     2701 tgacgattcg gcgcggggca agggcaagga tcgggtgctc gcctatctcg cgaaggttcg
     2761 gccggacatg acggtcctcg accatctggt cctcagccat ccgcacaagg accatgtcga
     2821 gatgatggat gacgtgctcg aattctacca ggtccgcaac gtctgggatt cgggcgccct
     2881 caacgacact tgcggctatc gtgccctgct cgaggcgatc atcgccgagc ccggcgtcgt
     2941 ctatcacaat tcgctgggca gcggcggcac ccacgacgtg gccgtgaagg atgcaggcac
     3001 ctgtcacggc aggaagcggc ccgcgacgac gctgcgggtg cctcggagca gccagatcac
     3061 caatgccccg gtcccgctcg gagccggtgc gacgatgacg attctcctcg ccgacggcag
     3121 cgtgaagatg ccgcgcggca atttcaacga ggccagcgtc gtcgcccggc tgctgctcgg
     3181 cacgcgcagc gtgctgcttc ccggggacgg cgaggccggc gggcgcaaga agcccagcga
     3241 tctccccaag gccaattccg ccgaagccaa gctgctcgcc tgctgcgcgc tgcagctgcg
     3301 ctcagacata ttggtggcca gccaccacgg cagcatgacc tcgtcccgaa acgcgttcct
     3361 ggatgcggtc ggcgccagcc agtatgtcgt gtcggtcggg cccaaggcct attcgggcac
     3421 cgtcctgccg gacagcgagg tcatcgacga atataacaag cgcggcaaat tgtggaccac
     3481 cgcgctcaac gacgcgacct gtccgaccag caggaagatc ggccccgaca aggacggacg
     3541 ccccggcggc tgcgacaacg tccgcatcgt catcggcccc gacggatcga tcgctccaga
     3601 ctatttccgg gcggtcgact gaccgcgccg gctgcgcatt atcgatttct cgtatatccc
     3661 ctcttcggat ataagcgtcc ccggcggttg ggcccgcgca gcgcggccac cgtcctgggg
     3721 ggacgcggcc gatgcgctgg atcatcgcga ttgcattcgc tttcttgtat gtcctggcgc
     3781 cgagcccggc ccgggcgtgg ggcgagagcg gccattacac cgtctgcgag atcgcctatc
     3841 taaacctgac gccgagggcg agggcggagg tcgaccggct gatcgcggcc ggcggcggct
     3901 atgacagctt cacccggtcc tgcacctttc cggacaagcc gcggcagcgc gccagcgagc
     3961 atttcgccaa ttacccgcgc aacgcggcgc gagtcgccgg ccccgcctgc cccggcggcc
     4021 ggccctgcat cgtccaggcg atcgagagcg acctggccgt cctccgctcg ccgacggcgt
     4081 cggacgacga caagtcgaaa gccctgaaat tcctcggcca ctggtatggc gacatccacc
     4141 agccgctcca catctcgttc gccgacgatc agggcggcaa cctcatcgat cagagcgggt
     4201 cgtgccagtt cagcctccac tcggtgtggg acacctgcat cgtcgagcgg cgcctgttcg
     4261 gcccgggcga tgaccgctat gcgcgcgctc gggccgccgc cgcggcgctg aaccgcacga
     4321 tcaccgcccg gcagcggcgc cagtgggcga ggacgcgccc gtggcaatgg gccggcgaat
     4381 cctacgagat cagccggcgg gccgcgaccg gctattgcct ccgcaaggcg cgcacgtgcc
     4441 gctattcggc gaccgccgcc gcctatgtcg agggcgccgc caagcgcgtc cagaccgtag
     4501 acgacgccta tctcgaccga gcccggcgaa tcgtcgccga ccggctgaag cgcggcggca
     4561 tccgcctcgc ccatgcgctg aaccgggcct tcgacccggc ctatcggggc tgagccggac
     4621 cggtgcgcgc gctcagcttt ctcgccgccg ccgcctcgct cgcgctcggc cccgcgtcgc
     4681 tcgatccctg cccctccgga agggccgccg cccgggccta tgcctgctgc aagatctgct
     4741 cgacgggcaa ggcctgcggc gacagctgca tcgcccgctg gaagacctgc cacaagggca
     4801 agggctgcgc ctgcgacggc tgagcgcctg agaccggttc cccgcaggcc gcccgaccgc
     4861 taagcggcgg ccatggccga cgcgcgaccc caacgacccc gctcggcgct gcgggccttc
     4921 ctgaagagcg aggcggccgg cggcgtgctt ctgatggcgg cggcggcagc ggctctggtc
     4981 gtcgccaatt cgccgctggc ctccgactat caggagctgc tccatcaacg ggtcggcccg
     5041 atgagcgtcc accattggat caacgacgcg ctgatggccc tgttcttcct gctcgtcggc
     5101 ctggagatca agcgcgagta cctcgacggc cgcctcgcca cctgggagcg gcggcggctg
     5161 ccgatcctcg ccgcggccgc cggaatggcg gtgccggccc ttctctacct cgccgtggcc
     5221 gggcgcgagc cggggctggc ggcgggctgg gcgatcccgg ccgcgaccga catcgccttc
     5281 gcgatcggag tcctcgcctt gctgggcaag agggcgccga cctcgctgaa gctgttcctc
     5341 accaccgtcg ccatcgcgga cgatctcgga gcggtcgcga tcatcgccct ggcctacacc
     5401 gactcgctcg acgcggccgc gctcggcgcg gcggcggcga tccttctcgt cctctacgcg
     5461 atgggcaaga gcggcgtccg ccggctgtgg ccgtacctga tcggcgccgg gctgctctgg
     5521 tacgccgtac ttctgtcggg cgtgcacgcc accgtcgccg gagtgctcgc ggccgccgcc
     5581 attcccatcg tcgccgcgcc cggcgcgccc gactcggcgg attcgcctct ccaccggct
//
LOCUS       DASVQZ010000045         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_108016, whole genome shotgun sequence.
ACCESSION   DASVQZ010000045 DASVQZ010000000
VERSION     DASVQZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108016"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VF547_01190"
     CDS             <1..1267
                     /locus_tag="VF547_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8261468.1"
                     /translation="FLDGSRFAEDQTVVVDKGKIVAVGPAASTRPPAGAKLIDGRGKT
                     LVPGLWDAHMHFFDDSSGPMLLSLGITSARDPGNVTALTLARRARREKGDLLSPKVYA
                     STLIDGKGPNTAQVAEVATTQEEAIEAVRRAKANGMTGVKFYGTFNPAWLPAAAAEAH
                     RLGLHVHGHVPAGMRTVDAINAGYDEVTHIYFMMMEAMPDEVVAVSNGIQRFQGPGRY
                     AKDVDLDGKAIRTLIETMARRKTVADPTLVVAESLFVPENGDLSPAYAPFLGTLPPAT
                     ERQFRQGGFAVPAGLTRADWRASYRKLAGLVGAMHKAGVPIVAGTDGSGMELVRELEL
                     YVQDAGFTPAEALAAATIVPARLVGADSRTGSIAVGKAADLVLVDGDPSRRIGDLRHT
                     QLVMMDGKLMDADALRAAGGFGGRPKFAE"
     gene            complement(1489..1752)
                     /locus_tag="VF547_01195"
     CDS             complement(1489..1752)
                     /locus_tag="VF547_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261469.1"
                     /translation="MPVSNLTLVERIARVLAGRYHSINADGDDPSAGPSVDETWHEYV
                     QDALSILRTMREPDQVMAAAGDAEVWERMVEAALSVERTVAGN"
     gene            1901..2365
                     /locus_tag="VF547_01200"
     CDS             1901..2365
                     /locus_tag="VF547_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEX8261470.1"
                     /translation="MQPSEACIKIIQKSESCAKRQPDGTYKAYPDPGSGGDPWTIGWG
                     STGPDIKPGTVWTQKQCDERFSEHVAQFAEAVTKAIGSAPTTQHQFDAMVSLAYNIGV
                     NAFAGSTVLRKHKAGDYKGAAAAFGMWNKAKGKVLNGLTTRRAAEAELYAKP"
BASE COUNT          433 a          870 c          865 g          368 t
ORIGIN      
        1 cttcctcgac ggcagccgct tcgccgagga ccagacggtc gtcgtcgaca agggcaagat
       61 cgtcgccgtc ggaccggcgg ccagcacccg cccgcccgcc ggcgccaagc tgatcgacgg
      121 caggggcaag acactcgtcc ccggcctgtg ggacgcgcat atgcatttct tcgacgacag
      181 ctccgggccg atgctgctgt cgctcggaat cacctcggcg cgcgatcccg gcaacgtcac
      241 cgcgctcacc ctcgcccggc gcgcgcgccg tgagaagggc gacctgctca gcccgaaggt
      301 ctatgcctcg accctgatcg acggcaaagg gccgaacacc gcgcaggtcg ccgaggtcgc
      361 gacgacgcag gaggaggcca tcgaggccgt tcgccgcgcg aaggccaacg gcatgaccgg
      421 ggtgaagttc tacggcacgt tcaacccggc ctggcttccg gccgcggccg cggaggccca
      481 caggctcggc cttcatgtcc acggccatgt cccggccggg atgcgcaccg tcgacgcgat
      541 caacgcgggc tatgacgagg tcacccacat ctacttcatg atgatggagg ccatgccgga
      601 cgaggtcgtc gccgtgtcca acgggatcca gcgcttccag ggtccgggcc gctacgccaa
      661 ggacgtcgac ctcgacggca aggcgatccg gacgctgatc gagacgatgg cgcgcaggaa
      721 gaccgtcgcc gacccgacgc tggtcgtcgc cgagagcctg ttcgtgccgg agaatggcga
      781 cctctcgccg gcctatgcgc cgttcctcgg cacgctgccg ccggcgaccg agcgccagtt
      841 ccgccagggc ggcttcgccg tgcccgcggg cctcacacgc gccgactggc gggccagcta
      901 caggaagctc gcgggcctgg tcggagcgat gcacaaggcc ggcgtgccga tcgtcgccgg
      961 caccgacggc agcggcatgg agctggtccg cgagctcgag ctctacgtcc aggacgcggg
     1021 cttcacgccg gccgaggcgc tggccgcggc gacgatcgtc cccgcgcggc tcgtcggggc
     1081 ggattcgagg accggctcga tcgcggtcgg caaggcggcc gacctggtgc tggtcgacgg
     1141 cgatccttcg cggcggatcg gcgacctccg ccacacccag ctggtgatga tggacggcaa
     1201 gctgatggac gccgacgcgc tccgagccgc cggagggttc ggaggaaggc cgaaattcgc
     1261 ggaatgatgt cctccccatg ctcagcatgg ggagggggac cacccgaagg gtggtggacg
     1321 ggtccttcga tacgccgctt cgacttcgct cagcggctac tcaggatgag cggttgtgct
     1381 cttccgctca tcctgagtag ggactgagcc tggcgaaggc ccgtatcgaa ggactcctcc
     1441 cccatgcttc gcatggtccc cctccccgcc gtttcccggc agggaggatt agttgcccgc
     1501 caccgtccgc tcgacgctga gcgccgcctc gaccatgcgc tcccagacct ccgcgtcgcc
     1561 ggcggcggcc atgacctggt ccggctcgcg catcgtccga aggatcgaca gcgcgtcctg
     1621 gacatattcg tgccaggtct cgtccacgct cggccccgcg gaggggtcgt cgccgtcggc
     1681 gttgatgctg tgatagcggc ccgcgagcac gcgggcgatg cgctcgacca gggtcaggtt
     1741 ggagacgggc atggacgaac ctccttcgtt tcgagcagac aacggaacaa gcgcccccgg
     1801 gttgcgacgg cgacggtccc tgccgacgct tgccgattga cagcccgata ggccggtata
     1861 cgttttccac attcaggccg gagtcgacgg ggaggtcgaa atgcagccga gcgaagcgtg
     1921 catcaagatt atccagaaat ccgaaagctg cgccaagcgg cagccggacg ggacctacaa
     1981 ggcctatccg gaccccggct cgggcggcga tccctggacg atcggctggg gctcgaccgg
     2041 gcccgacatc aagccgggga cggtgtggac ccagaagcag tgcgacgagc gcttcagcga
     2101 gcatgtcgcg caattcgcgg aggccgtcac caaagccatc ggctcggctc cgaccaccca
     2161 gcatcagttc gacgcgatgg tcagcctcgc ctacaatatc ggagtcaacg cctttgccgg
     2221 cagcacggtg ctgaggaagc acaaggccgg cgactacaag ggcgcagccg ccgccttcgg
     2281 catgtggaac aaggccaagg gcaaggtgct gaacggcctc accacgcgaa gggcggccga
     2341 ggcggagctt tacgcgaagc cgtaacgggt cgaaggatcg gcccccacct cccggacgcc
     2401 gttcgccctg agcctgtcga agggcctgcc ttgctgctca caaaggaaaa gggagggctt
     2461 cgacgggctc agcccgaacg gggagaacga atccgagagc gctcggcagt ctgattgcgc
     2521 tgcttccgtt cgggct
//
LOCUS       DASVQZ010000046        13341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_587762, whole genome shotgun sequence.
ACCESSION   DASVQZ010000046 DASVQZ010000000
VERSION     DASVQZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13341
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587762"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..446)
                     /locus_tag="VF547_01205"
     CDS             complement(204..446)
                     /locus_tag="VF547_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX8261471.1"
                     /translation="MAEGTIAADELRLLIERIERLEEEKKAIADGVTDVYGEAKARGY
                     DTKTMRAIVRLRKMETHDRQEADALLETYKAALGLV"
     gene            507..2000
                     /gene="pyk"
                     /locus_tag="VF547_01210"
     CDS             507..2000
                     /gene="pyk"
                     /locus_tag="VF547_01210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX8261472.1"
                     /translation="MANSETLPSKFVPRTRKVRILATLGPASRSPEMIRKLAEAGADA
                     FRINMSHGTHKDHLTLIETIRALEKELDRPTTILADLQGPKLRVGRFEGDGVDLEKGQ
                     TFVFDRDPAPGTSKRAELPHREIFGGVEKGTRLLLDDGKLVQRVVSATADRIETRVEV
                     GGRLSNNKGVNVPDVVLPLAALTDKDRADLAFALDQHVDWIALSFVQRPEDVAEARRL
                     IAGRAALLAKIEKPAAVDRIDGILELADAVMVARGDLGVELPPEAVPPLQKMIVEAAR
                     RFGRPVVVATQMLESMIEAPTPTRAEVSDVATAVYDGADAVMLSAESASGKYPCEAVT
                     MMNSIATSVENDPSYSERVHFTRTLPDPTTADAIAQAANTIVGTVDAKAIVCFTSSGS
                     TARRLSRERPAVPLLVLTPSGKTARKLGILWGAHAVRTRDIANFEEMIAKAKRMALRH
                     GVARGGDRIVIIAGVPFGTPGSTNVIHIVRLVGDELERHRKTQEKLH"
     gene            complement(2128..3006)
                     /locus_tag="VF547_01215"
     CDS             complement(2128..3006)
                     /locus_tag="VF547_01215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX8261473.1"
                     /translation="MSDFEVVDCDLLRRFPLPRHPEDGDKEDRGRLLVIAGSRELAGA
                     ALLAGTGGLRAGAGKLQIATGASVAPALAVAVPEARVVGCPETDEGCIAAEAIPPLLE
                     WAAGAQAILIGCGLQHGPPLETMLDSLLASGLDKPLVLDAAVLGSLAPRAATLRAWRG
                     GAILLPHAREMARLLECEPEAVEADPLAAARRAAETFGAVALIKGQYSYIVAPHGRAF
                     RFTGGGVGLATSGSGDALAGIVGGLCARGADPLAAALWGVHLHGEAGRKLAKRIGRIG
                     FLARELLDEVPGLMED"
     gene            complement(3003..3767)
                     /locus_tag="VF547_01220"
     CDS             complement(3003..3767)
                     /locus_tag="VF547_01220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8261474.1"
                     /translation="MRQRWPASLWIVRHGQSAGNVARDAADAAGLGMIDLDIRDVDVP
                     LSDLGHRQADALGRWFAALPEEERPEVVLTSPYVRAQQTAAAICAAGGTASGAKGPVK
                     DERLREREFGLWDRLTTAGVREKFPDQAEQRAVLGKFYHRPPGGESWADVILRLRSAL
                     DTVSLHHADRRVLIVCHQVVVLCFRYILEELDEAQILAIDKEGDVVNCGLLRYAFEPD
                     DEKDCVPKLVCYNFAAPLIEEEAPITSEPDAMVAAR"
     gene            complement(4013..4138)
                     /gene="ykgO"
                     /locus_tag="VF547_01225"
     CDS             complement(4013..4138)
                     /gene="ykgO"
                     /locus_tag="VF547_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX8261475.1"
                     /translation="MKIRNSLKSLKSRHRDCRVIRRRGRTYVINKTNRRFKARQG"
     gene            complement(4213..5193)
                     /locus_tag="VF547_01230"
     CDS             complement(4213..5193)
                     /locus_tag="VF547_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8261476.1"
                     /translation="MATEAEISDLLVRGRAYAEAGDSRAAASFYAAALRAAAAAPALP
                     APLLARLGTAQSYLREAAARYQAHLERAVAGAGAGPRLDQALAIMLGREPLHLPPVAY
                     PQRPSVFHFPGLPPRPFYERELFAWAPALEAATPAIRSEVQALLSEGADFRPYVEAEK
                     DRPQRDFHGLQGDPSWTAFYLWKDGGLVEANAERCPRTVEAMRRVPMSAIGSRTPSVF
                     FSLLRPGAHIPPHHGMLNSRLICHLPLIVPPGGWLRVGAETRLWREGELLVFDDSIEH
                     EAMNPAAALRVVLIFDIWRPELTEPERRGISAIFDAIDAYSPAAAPQQDA"
     gene            complement(5243..6424)
                     /locus_tag="VF547_01235"
     CDS             complement(5243..6424)
                     /locus_tag="VF547_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8261477.1"
                     /translation="MELSNQEAERLVREAVDAFQQGRPGEARARLDRVAATGRANAQI
                     WLLLATVCRALRDVSGEEAALDRVLEMEPRAIRALIMKADCRAAAADYRIAASLYKKA
                     RNIAASERLPPGQTLPADLVAELRRAEAAAAGLDARYSVHIESSLAAQGLPPENRSYR
                     FQQSIDIMSDRGRIFLQEPTGYYFPELPHVQFYDREDFDWVPSIEAATDVIREELLAV
                     MATGIGDFCPYIRPEPNQPRHHPLFNKTEWSALFFVENGRLSEETIARCPETWKAVQA
                     APLPWIAGWEPTVMFSLLRAKTAIPPHSGTHNTRLICHLPLIVPPDCGFRVGNQIRQW
                     EEGKLLLFDDTIEHEAWNNSSEDRVVLIFGIWRPELSDRERHEVSALFSVPSDEEETP
                     S"
     gene            complement(6433..7437)
                     /gene="galE"
                     /locus_tag="VF547_01240"
     CDS             complement(6433..7437)
                     /gene="galE"
                     /locus_tag="VF547_01240"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX8261478.1"
                     /translation="MSKPSVLVTGGAGYIGSHAVLALLDAGWKVAVIDNLVTGFRWAV
                     PDEAVFAEGDIADQALVGRLIEEQGIGAIIHFAGSIVVPESVENPLKYYENNTAKSRS
                     LIESAVRGGVRHFIFSSTAATYGIPESVPVVETSRTQPINPYGWSKLMTERMLADVAA
                     AHPLNFCALRYFNVAGADPRGRSGQSTAGATHLIKVAVEAAIGKRDSVSVYGTDYDTP
                     DGTGVRDYIHVSDLAAAHVHALEKLIEDPGASHIMNCGYGRGFSVLEVLDAVDRVTNV
                     RIDRRMEARRAGDPDTLVADNAKILATLPWQPKLDDLDTIVAHALAWERHLAERPVSS
                     "
     gene            7536..8114
                     /locus_tag="VF547_01245"
     CDS             7536..8114
                     /locus_tag="VF547_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX8261479.1"
                     /translation="MKSAFLRSAPLVLAASLAACAGGGGGKAPIGSGVEVARFHLGDP
                     VARAQIAVEPFDKADANRPEFPAYAAAVGRQLSRLGWQVVPTATNSEQIALIDLEQGS
                     REAVAALSAARIGRGLSPAAPAGTSANVTATLLEVAIRRRSDGTVFWEGRAVGEARTD
                     SPQAARTAAVERLAEALFRDFPGESGRTIRVK"
     gene            8111..9244
                     /locus_tag="VF547_01250"
     CDS             8111..9244
                     /locus_tag="VF547_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEX8261480.1"
                     /translation="MTLSVTSPFDGGNIRLVAIAGDRIDLEIAKDNQSDFYQWFYFRL
                     TGGAGRAVELRILNCAGAAYPLGWEDYRACMSSDREEWERVDTAYEDGVLSIKATPPS
                     NSVWFAYFAPYTMERHHDLVSSVAAHPLVEHRSLGQTLDGQEMDYFRIGEGPLQVWLY
                     ARQHPGETMAEWWMEGALERLLDEADPVARRLLAKATFHIVPNMNPDGSRRGHLRTNA
                     VGTNLNREWHDPSPERSPEVLCVRNEMDRTGVDFAMDVHGDEAIPANFLAGFEGIPSW
                     NEAQQKRFDRFAEELVRFSPDFQTRKGYEISKPGEANMSMSTSQLAERYGAVSMTLEM
                     PFKDNEDLPDPRRGWSPERSALLARACLDALHAVIDELAPRKT"
     gene            9361..10047
                     /gene="gpmA"
                     /locus_tag="VF547_01255"
     CDS             9361..10047
                     /gene="gpmA"
                     /locus_tag="VF547_01255"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX8261481.1"
                     /translation="MPILVLIRHGQSAWNLENRFTGWWDVDLTPKGVEEARLAGELMA
                     GKGLDFDLCFTSLQTRAIKTLNLALEAMGRLWLPVEKDWRLNERHYGGLTGLNKAETA
                     ARHGDEQVHVWRRSFDTPPPPMEPGGPYDLSGDRRYSGIAVPRTESLKDTIARVLPYW
                     ESRIVPALQRGQRVLISAHGNSLRALVKHLSGIPDDEITGLEIPTGQPIVYRLDAGLA
                     AIDRYYLSER"
     gene            10058..11023
                     /locus_tag="VF547_01260"
     CDS             10058..11023
                     /locus_tag="VF547_01260"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8261482.1"
                     /translation="MVDEPVSELRRRAGDGDGEARLALGLRLLGDDGDAAEGAALIEA
                     ECEAGSAEAAALLATLEAMGAARPQSWTRALDYLELAACRGSRRARAQLAMLGRNEAE
                     QDWKRLRAAVDVAELTAAPPRRPVSEQPRIRTFERFASPAECDWVIAIARGRLNPAWV
                     WDPLTGRNRPDPSRTNRGLDLGVAEMDLVVQILRARISTASNLPLPVFEPAQIMHYSV
                     GQEFRPHHDYLDPGQPGDREQIARLGQRIATFLLYLNDDYEGGETEFLKTGFRYRGGK
                     GDALLFANVDAGRAPDPLTTHAGRPPTRGEKWILSQWIRDRSPGP"
     gene            11224..12201
                     /locus_tag="VF547_01265"
     CDS             11224..12201
                     /locus_tag="VF547_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8261483.1"
                     /translation="MAFDARFEPLAGVPRAPADPRVELGIELLGERGAESRGAALIEA
                     AFQAGDSEAAAVLATIEAMGAGRPQSWPRALELLEIAALRGSERARAQLRTLGRSNSS
                     DWSALRSGADLAALAAPPVRRPVSERPRIRVIEGFATPGECDWVIAAARGKLRPAQVW
                     DPSTGEGRDVPNRTNRALELGLADMDVVLQLLRTRIAPASNLPLPVFEPAQIMQYRSG
                     EEFRPHYDFLDPDQPGHAPELARFGQRIATFLLYLNDDFEGGETAFPKTGFKYRGRRG
                     DALLFANVDAATRAPDPLTLHAGLPPMRGEKWIFSQWIRDRSPGPPAGG"
     gene            complement(12198..12773)
                     /locus_tag="VF547_01270"
     CDS             complement(12198..12773)
                     /locus_tag="VF547_01270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8261484.1"
                     /translation="MSKHASDFAGLPVETSDENALLRAALAEAQQRIVELERNAESDT
                     VTPLPSARRFRAELERVVGLAERHRTPAAVVGIELTSLAEIHESHGHFAADAALVHVA
                     RLLCGLIRSTDVLARTGDNRFGLILDHLDHNSAIDTAERLARCIAANPLDLGDVRVKL
                     EAKVATTGILPGDGVEDVLLRADRNLALADA"
     gene            12855..13331
                     /gene="purE"
                     /locus_tag="VF547_01275"
     CDS             12855..13331
                     /gene="purE"
                     /locus_tag="VF547_01275"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX8261485.1"
                     /translation="MAEVGIIMGSRSDWETMRHAADMLDALEIAYETKVVSAHRTPER
                     LYDYARGAAGRGLKVIVAGAGGAAHLPGMAASMTRLPVLGVPVRSRALDGLDSLLSIV
                     QMPAGVPVGTLAIGQAGASNAGLLAASILALSDEALAGRLEAWRQRQSDGVAEAPE"
BASE COUNT         2071 a         4613 c         4642 g         2015 t
ORIGIN      
        1 cgcccttgcg atacatgatg ttcttgaact tgctgtggcc ggccatcggg tccctctaat
       61 ccgttcgggc tgagcctgtc cttcgatacg ggccttcgcc ttcgctcagt ccctactcag
      121 gatgagcgga aaggaaagac agccaatcgc catccgctcg tcctgagtag ccgtcgagcc
      181 tggcgaaacg gcgtatcgat ggatcacacc agccccagcg ccgccttgta ggtctcgagc
      241 agggcgtcgg cttcctggcg gtcgtgggtc tccatcttgc gaaggcggac gatcgcgcgc
      301 atcgtcttgg tgtcgtagcc gcgggccttg gcctcgccgt agacgtccgt cacgccgtcc
      361 gcgatcgcct tcttctcctc ctcgagccgc tcgatccgct cgatcaggag cctcagttcg
      421 tcggccgcga tcgttccttc cgccatttga tccttcttcc atccggggtt gagccgcggg
      481 cttaggggcg gctatgcgcg gcttcaatgg ccaattccga aacgctcccc agcaaattcg
      541 tcccccgcac ccgcaaggtg cggatcctcg ccacgctggg gccggccagc cgaagcccgg
      601 agatgatccg caagctcgcc gaggcgggcg cggacgcctt ccgcatcaac atgagccacg
      661 gcacccacaa ggaccatctc acgctgatcg agacgatccg ggcgctcgag aaggagctcg
      721 accggccgac gaccatcctc gccgacctgc agggacccaa gctcagggtg gggcgattcg
      781 agggcgacgg agtcgatctc gagaagggcc agaccttcgt cttcgaccgc gatccggccc
      841 cgggcacgtc gaagcgggcg gagcttcccc atcgcgagat attcggcggg gtcgagaagg
      901 gcacccggct gctgctcgac gacggcaagc tcgtccagcg ggtcgtgagc gcgaccgccg
      961 accggatcga gacccgggtc gaggtcggcg gccgcctgtc caacaacaag ggggtcaacg
     1021 tccccgacgt ggtgctcccg cttgccgcgc tgaccgacaa ggaccgcgcc gacctcgcct
     1081 tcgcgctcga ccagcatgtc gactggatcg ccctttcgtt cgtgcagcgg cccgaggacg
     1141 tcgccgaggc gcggcgcctg atcgcggggc gcgccgcgct gctcgccaag atcgagaagc
     1201 cggccgcggt cgaccggatc gacggcatat tggagctggc cgacgcggtg atggtcgcgc
     1261 gcggcgacct cggcgtcgag cttccgcccg aggcggtgcc gccgctccag aagatgatcg
     1321 tcgaggcggc ccggcgcttc ggccgcccgg tggtggtggc gacccagatg ctcgaatcga
     1381 tgatcgaggc gccgaccccg acccgtgccg aggtctccga cgtggcgacc gccgtctacg
     1441 acggcgccga cgcggtgatg ctttcggcgg agagcgcctc gggcaaatat ccctgcgagg
     1501 cggtgacgat gatgaactcg atcgccacca gcgtcgagaa cgacccgagc tattccgagc
     1561 gggtccactt cacccgcacc ctgcccgatc cgaccaccgc cgacgccatc gcccaggcgg
     1621 cgaacacgat cgtcggcacg gtcgacgcca aggcgatcgt ctgcttcacc tcgtccggct
     1681 ccaccgcccg acggctgtcg cgcgagcggc cggcggtgcc gctgctggtg ctcaccccgt
     1741 cggggaagac ggcccggaag ctcggcatat tgtggggcgc gcatgccgtc aggacccgcg
     1801 acatcgccaa tttcgaggag atgatcgcca aggccaagcg gatggcgctc cgccacggcg
     1861 tggccagggg cggcgaccgt atcgtgatca tcgccggggt gccgttcggg actccgggct
     1921 cgaccaacgt catccatatc gtccggctgg tcggcgacga gctcgagcgg caccggaaga
     1981 cccaggagaa gcttcactag aaatcccgcc tacgatttcg cggggagcgg accgcgcgca
     2041 gcgcggtgga ggggtcttcg gaaccagcaa ggcgcctccc accctgctgc gcctggtccc
     2101 cctcccctgc aatgcagggg aggattttca gtcctccatc aggcccggca cttcgtcgag
     2161 aagctccctc gcgaggaagc cgatccggcc gatgcgcttg gccagcttgc ggccggcctc
     2221 gccgtggagg tggacgcccc acagggccgc ggcgagcgga tcggcccccc tggcgcacaa
     2281 tccgccgacg attccggcaa gggcgtcgcc cgatcccgag gtcgcgaggc ccactccgcc
     2341 gccggtgaag cggaaggcgc ggccgtgcgg ggcgacgatg tagctgtatt ggcccttgat
     2401 cagggcgacc gccccgaacg tctcggcggc gcggcgggcg gcggcgagcg ggtcggcctc
     2461 caccgcctcc ggctcgcatt cgagcagccg cgccatctca cgggcatggg ggaggaggat
     2521 ggcgccgccg cgccacgccc gcagggtcgc ggcccgaggc gcgaggctgc cgagcacggc
     2581 cgcgtcgagc accagcggct tgtcgaggcc gctcgccaac agcgaatcga gcatcgtctc
     2641 gagcggcggg ccgtgctgga gcccgcagcc gatcaatatg gcctgggcgc ccgcggccca
     2701 ttccagcaag ggcgggatcg cttcggccgc gatgcagccc tcgtccgtct ccggacagcc
     2761 gacgacgcgc gcttcgggca cggcgaccgc gagcgccggg gcgacgctgg cgccggtggc
     2821 gatctggagc ttgccggcgc ccgcgcgcaa tccgccggtg ccggcgagca gcgccgcgcc
     2881 cgccagctcg cggctgccgg cgatgacgag caggcggccg cgatcctcct tgtccccgtc
     2941 ttcgggatga cgtgggagcg ggaaacggcg caggagatcg cagtcgacga cctcgaaatc
     3001 gctcatcgcg cggcgaccat ggcgtcgggc tcggacgtga tcggcgcctc ctcctcgatc
     3061 agcggcgcgg cgaaattgta gcagaccagc ttgggcacgc agtccttctc gtcgtcgggc
     3121 tcgaacgcgt agcggaggag tccgcaattg acgacgtcgc cctccttgtc gatcgccagg
     3181 atctgcgcct cgtccagctc ctccaatatg taacggaagc acaggaccac gacctggtgg
     3241 cagacgatca ggacgcggcg gtcggcgtgg tggaggctga ccgtgtcgag agcgcttcga
     3301 aggcgcagga tgacgtcggc ccagctctcg ccgcccggag gccgatggta gaatttgccg
     3361 agcaccgcgc gctgctcggc ctggtccggg aatttctcgc gcaccccggc cgtcgtcagc
     3421 cggtcccaaa ggccgaattc gcgctcgcgc aggcgctcgt ccttgaccgg ccccttggcg
     3481 ccggaggcgg tgccgcccgc cgcgcagatc gccgccgcgg tctgctgcgc gcggacgtag
     3541 ggcgaggtga gcacgacctc gggccgctct tcctcgggca gggcggcgaa ccagcggccg
     3601 agcgcgtcgg cctgacgatg gccgaggtcg ctcagcggca cgtcgacgtc acggatgtcg
     3661 aggtcgatca ttcccaggcc ggcggcatcg gcggcgtcgc gcgcgacatt gccggcgctc
     3721 tgcccgtggc ggacgatcca caaactggcg ggccatcttt gtctcatcgc cgaaatcaaa
     3781 agcgcgagcg ggatggaagt tgcgtccgcg ccccgcatcg agcggggggg ggggggggga
     3841 gccggcgcat ggcgctcgcc ggccgcggcc ctggatcgtc ggcgttggcg aggctgcggg
     3901 cacgctcacc cgttcggccc ggcctgtcga agcccgacct ttcctctctc gggaacaacg
     3961 aaagcccttc gacgggctca gggcggaccg agcgggtctc ggcccccgga cgtcagccct
     4021 ggcgcgcctt gaagcggcgg ttggtcttgt tgatgacgta ggtgcggccc cggcggcgga
     4081 tcacgcggca gtcgcggtgg cgcgacttga gggacttcag ggagttgcgg atcttcatgg
     4141 ccttgaggac ctgtatcttt cggattgtgc cggaaaaacg aggcgttccc ctaggcggca
     4201 ggcctcgcca agtcaagcgt cctgctgcgg cgcagcggcg ggcgaataag cgtcgatcgc
     4261 gtcgaaaatc gccgagatgc cgcgccgctc cggctcggtc agctcgggcc gccagatgtc
     4321 gaagatgagg acgacacgaa gagccgcggc cgggttcatc gcctcatgct cgatgctgtc
     4381 gtcgaagacc agcagctcgc cttcccgcca gagccgggtc tcggcgccga cgcgcaacca
     4441 tccgccgggg ggcacgatca acggcaaatg gcagatcagc cgggaattga gcatgccgtg
     4501 gtgcggcgga atatgggcgc cgggccgaag cagcgagaag aagaccgacg gggtgcgcga
     4561 cccgatcgcg ctcatcggca cccgccgcat ggcctcgacg gtgcgcgggc agcgctcggc
     4621 attcgcctcc accaggccgc cgtccttcca aagatagaaa gccgtccagc tcggatcgcc
     4681 ctggaggccg tggaagtcgc gctgcggacg gtccttctcg gcctcgacat aagggcggaa
     4741 gtcggcgcct tcggagagca gggcctggac ctcgctccgg atggccggcg tcgcggcctc
     4801 cagagccggc gcccaggcaa agagctcgcg ctcgtagaag ggacggggag gcaggcccgg
     4861 aaagtgaaag acgctcggcc gctgcggata ggcgaccggc ggaagatgca gcggctcgcg
     4921 gcccagcatg atcgcgagcg cctggtcgag ccgcggcccg gcaccggccc cggcaacggc
     4981 gcgctcgaga tgggcctggt accgggcggc ggcttcgcga agataggatt gggcggtgcc
     5041 cagccgggcg aggagcggag cgggaagagc cggggcggcg gctgcggcgc gcagcgcggc
     5101 ggcatagaag ctggcggcgg ctcgggagtc cccggcctcg gcataggctc ggccccggac
     5161 gagcagatcc gagatttccg cctcggtggc cacgctcagc ttcgccccgc cgaccggcct
     5221 tccagaggtc gcggcggacc cgtcacgacg gcgtctcctc ctcgtccgag ggcacggaga
     5281 aaagcgcgga tacctcgtgc ctctcgcggt cgctcagctc cggtctccag atgccgaaga
     5341 tcagcacgac gcggtcctcg gaactgttgt tccaggcttc gtgctcgatc gtgtcgtcga
     5401 acagcagaag cttgccctcc tcccactgcc ggatctggtt gccgactcgg aagccgcagt
     5461 cgggcggcac gatcagcggc aggtggcaga tcagccgggt attgtgggtg ccgctgtggg
     5521 gcgggatcgc cgtcttcgcg cggagcagcg agaacatgac ggtcggctcc cagccggcga
     5581 tccacggcag gggcgcggcc tggacggcct tccaggtttc cgggcagcgc gcgatcgtct
     5641 cctcggacag ccggccgttc tcgacgaaga aaagcgcgct ccactcggtc ttgttgaaca
     5701 agggatggtg gcgcggctgg ttcggctcgg gacggatgta ggggcaaaag tcgccgatcc
     5761 ccgtcgccat gaccgccagc agctcctcgc ggatgacgtc ggtggccgcc tcgatcgaag
     5821 gcacccagtc gaaatcctcg cgatcgtaga actgcacatg gggcagctcg gggaaataat
     5881 agccggtcgg ctcctgcagg aagatgcgtc cgcggtccga catgatgtcg atcgactgct
     5941 ggaagcggta gctgcggttc tcgggcggca gtccctgcgc cgccagcgag ctctcgatgt
     6001 ggacggaata gcgtgcatcg agcccggccg ccgccgcctc ggcccggcga agctccgcca
     6061 ccaggtccgc aggcagggtc tggccgggcg gaaggcgctc gctcgcggcg atgttcctcg
     6121 ccttcttgta gagcgaggcc gcgatccggt aatcggccgc ggcggcgcgg caatcggctt
     6181 tcatgatcag ggcccggatc gcccgcggct ccatctccag cacccggtcg agcgcggcct
     6241 cctcgccgga aacgtcgcgc agcgcgcggc agacggtggc gagcagcagc cagatctggg
     6301 cgttggcgcg ccccgtcgcc gcgacccgat cgaggcgggc acgcgcctcg cccgggcggc
     6361 cctgctggaa ggcgtcgacg gcctccctga cgagcctttc cgcctcttga ttgctgagtt
     6421 ccatggcgcc ttctaggaag agaccggtcg ttccgccaga tgcctctccc aggcgagggc
     6481 atgggcgacg atggtgtcga gatcgtccag cttcggctgc cagggaagcg tggccaggat
     6541 cttcgcattg tccgcgacga gcgtgtccgg atcgcccgcg cgccgcgcct ccatacgccg
     6601 atcgatccgc acgttggtga cccggtcgac cgcgtcgagc acttccagaa ccgaaaagcc
     6661 gcggccgtag ccgcagttca tgatgtggct ggcgcccgga tcctcgatca gcttctcaag
     6721 cgcatggaca tgggccgcgg cgaggtcgct gacgtggata tagtcgcgga cgccggtgcc
     6781 gtccggagtg tcgtagtcgg ttccgtagac cgacaccgag tcgcgcttgc cgatcgccgc
     6841 ctcgaccgcg accttgatca aatgggtcgc cccggcggtc gactggcccg agcggccccg
     6901 cgggtcggcg ccggcgacgt tgaagtagcg cagcgcgcag aagttgagcg gatgcgcggc
     6961 ggcgacgtcg gcaagcatcc gctcggtcat cagcttcgac cagccatagg ggttgatcgg
     7021 ctgggttcgc gacgtctcca ccaccggcac gctctcgggg atgccatagg tcgcggcggt
     7081 tgaggagaag atgaaatggc ggactccgcc cctcaccgcg ctctcgatca gcgagcggct
     7141 cttggccgtg ttgttctcat aatatttgag cggattttcg accgattccg gcaccacgat
     7201 ggagccggcg aaatggatga tcgcgccgat gccctgctcc tcgatcagcc ggccgaccag
     7261 cgcctggtcc gcaatgtccc cctcggcgaa gacggcttcg tccggcacgg cccagcggaa
     7321 gccggtgacg agattgtcga tcaccgcgac cttccagccg gcgtcgagca gcgccagcac
     7381 cgcatggctg ccaatatagc cggcgccgcc ggtcaccagc acggaaggct tgctcatcta
     7441 acctcttccc tcaaggaacc cgaatgcggc aaagagcgct ggacaggtca cggtccgcgc
     7501 cccacggtcc gcgcgctaga cgaagaggtg ccggaatgaa atcagctttt ctgcgcagcg
     7561 ccccgcttgt ccttgccgcg tcgcttgccg cctgcgccgg tggcggcggc ggcaaagcgc
     7621 ccatcgggag cggcgtcgaa gtcgcgcgct tccacctcgg cgaccccgtc gctcgggccc
     7681 agatcgcggt cgagccgttc gacaaggccg atgccaaccg gcccgaattc ccggcttatg
     7741 ccgccgcggt cgggcgccag ctcagccggc tcggatggca ggtggtgccg accgccacca
     7801 attcggagca gatcgccctc atcgatctcg agcagggcag ccgcgaggcg gtggccgctc
     7861 tcagcgcggc gcgcatcggc cgcggcctgt cgcccgcggc gcccgccgga acctccgcca
     7921 acgtcaccgc caccctcctc gaagtggcca tccggcgccg ctcggacggc accgtcttct
     7981 gggagggccg tgccgtggga gaggcccgca ccgacagccc gcaggcggcc cggaccgccg
     8041 ccgtcgagcg gctcgccgaa gccctgtttc gggacttccc cggcgagtcg ggccgcacta
     8101 tcagggtcaa atgaccctct ccgttacgag ccccttcgac ggcggcaaca ttcgcctggt
     8161 cgccatcgcg ggcgaccgca tcgacctcga gatcgccaag gacaaccagt ccgatttcta
     8221 ccaatggttc tatttcaggc tcaccggcgg cgccggccgg gcggtggagc tgcgcatcct
     8281 caactgcgcc ggagccgcct atccgctggg ctgggaggat taccgcgcct gcatgtcgag
     8341 cgaccgcgag gaatgggagc gggtcgacac cgcctatgag gacggcgtcc tgtccatcaa
     8401 ggcgactccg ccttccaaca gcgtctggtt cgcctatttc gcgccctaca cgatggagcg
     8461 ccaccacgat ctggtctcat cggtcgccgc ccatccgctg gtcgagcacc ggtcgctcgg
     8521 ccagacgctc gacggccagg agatggacta tttccggatc ggcgagggac ccctccaggt
     8581 ctggctctac gcccgccagc atcccggcga gacgatggcc gaatggtgga tggaaggcgc
     8641 gctcgagcgg ctgctcgacg aggccgaccc ggtcgcccgc cggctgctcg ccaaggcgac
     8701 cttccacatc gtccccaaca tgaatcccga cgggtcgcgg cgcggccacc tgcggaccaa
     8761 tgccgtgggc accaacctca accgcgaatg gcacgacccc agcccggagc gcagcccgga
     8821 ggtgctgtgc gtccgcaacg agatggaccg gaccggagtc gacttcgcca tggacgtcca
     8881 cggcgacgag gcgatccccg cgaatttcct cgccggcttc gagggcattc cgagctggaa
     8941 cgaggcgcag cagaaacgct tcgatcgctt cgccgaggag ctggtccgct tctcgcccga
     9001 tttccagacc cgcaagggct atgagatctc gaagcccggg gaagccaata tgtcgatgtc
     9061 gacctcgcag ctggcggagc gctacggcgc ggtgtcgatg acgctggaga tgccgttcaa
     9121 ggacaatgag gacctgcccg atccgcgccg cggctggtcg ccggagcgaa gcgccctcct
     9181 cgcccgcgcc tgcctcgacg cgctccacgc cgtgatcgac gagctggcgc cgaggaagac
     9241 ttaaggggcc gtcattgcgg gtggagcgaa gcaatccagt gcggcgtgcg aggctccgga
     9301 ttgcttcgtc gcttcgctcc tcgcaacgcc agctcagccg aacggagttt ccttcctgaa
     9361 atgccgatcc tcgtcctcat ccgccacggc cagtcggcgt ggaacctcga gaaccggttc
     9421 accggctggt gggacgtcga tctgactccc aagggggtcg aggaagcgcg gctcgccggc
     9481 gagctgatgg cggggaaggg cctggacttc gacctgtgct tcaccagcct ccagacccgc
     9541 gcgatcaaga ccctaaacct cgccctggaa gcgatgggac ggctctggct tccggtcgag
     9601 aaggactggc ggctgaacga gcgccattac ggcggcctga ccggcctcaa caaggcggag
     9661 accgcggccc ggcacggcga cgagcaggtt cacgtctggc gccggtcgtt cgacaccccg
     9721 ccgccgccga tggagcccgg cggaccctat gacctttccg gcgatcgccg ctattccgga
     9781 atcgcggtgc cgcgcaccga gagcctgaag gacacgatcg cgcgggtgct gccatattgg
     9841 gaaagccgga tcgtgcccgc cctgcagcgg ggccagcggg tcctgatctc ggcccacggc
     9901 aattcgctgc gggcgctggt caagcatctt tccggtattc ccgacgacga gattacgggc
     9961 ctcgagatcc ccaccggaca gccgatcgtc taccggctgg acgcggggct ggcggcgatt
    10021 gaccgctatt acctgagcga gcggtgatag gacggatatg gtcgacgagc ctgtctccga
    10081 gctgcgccgg cgggccgggg acggagacgg cgaggcccgg ctcgcgctcg gcctgaggct
    10141 gctgggcgac gacggagatg ccgccgaagg cgccgcgctg atcgaagccg aatgcgaggc
    10201 cggcagcgcc gaagcggccg ctctcctcgc gacgctcgaa gcgatgggcg cggcgcgccc
    10261 gcaaagctgg acccgggcgc tcgactatct cgagcttgcc gcctgccgcg gctcccgccg
    10321 tgcgcgcgcc cagctggcga tgctcggccg gaacgaagcg gagcaggact ggaagcgcct
    10381 tcgagccgcc gtcgatgtcg ccgagcttac cgccgcgccg ccgcgacggc cggtttccga
    10441 gcagccgcgc attcgcacgt tcgaaaggtt cgcctctccc gccgagtgcg attgggtgat
    10501 cgccatcgcc cgcggccgac tgaaccccgc ctgggtgtgg gatccgctga ccggccggaa
    10561 ccgccccgat ccgagccgga ccaatcgcgg cctcgacctc ggcgtcgccg agatggacct
    10621 ggtcgtgcag attctccgcg cccgaatctc gacggcgagc aatctgccgc tgccggtctt
    10681 cgagcccgcc cagatcatgc attactcggt cggccaggaa ttccggccgc atcacgatta
    10741 tctcgatccg ggtcagcccg gcgatcggga gcaaattgcc cgtctcggcc agcgcatcgc
    10801 gactttcctc ctctatctga acgacgatta cgagggcggc gagaccgagt tcctcaagac
    10861 cggcttccgc tatcgcggcg gcaagggaga cgcgctgctg ttcgccaatg tcgacgccgg
    10921 gcgcgcgccg gatcccctca ccacccacgc cggacgcccg ccgacgcggg gcgagaaatg
    10981 gatcctgtcg caatggatcc gggaccgttc gcccgggccc tgagtcgttc ggcggcggtg
    11041 ccgtttgtcg aaaaaagccg gtcgttcaaa gaacgtcctg aaatggcgtt gaacccctcg
    11101 ctaggttggc ggccgatgta ggcgggggga gagccaaatg cctcagcgat gctgctcgtt
    11161 cgataatctc gcgccggcgg gcgaccggcg ttccggctgc atctcctgat ctcaagcccg
    11221 gtcatggctt tcgacgcgcg tttcgagccg ctcgccggcg tgccgcgggc gccggcagac
    11281 cctcgggtcg agctcgggat cgagctgctg ggcgagcggg gcgcagaaag ccgcggcgcg
    11341 gcgctgatcg aggcggcgtt ccaagcgggc gacagcgagg cggcggcggt cttggcgacg
    11401 atcgaggcaa tgggggccgg ccggccgcaa agctggccga gggcgctcga gctcctcgag
    11461 attgcagcgc tgcgggggtc ggaacgagcc cgggcccagc ttcggacgct gggccgctcc
    11521 aattcctcgg actggagcgc gctgcgctcc ggggcggacc ttgcggcgct cgctgcgccg
    11581 ccggtccgcc gaccggtttc ggagcggccg cgaatccgcg tgatcgaggg attcgcgacc
    11641 cccggggaat gcgattgggt catcgccgcg gcgcggggca agctccggcc ggcgcaggtc
    11701 tgggatccgt ccacgggcga gggacgagac gttccgaacc gcaccaaccg ggctctggag
    11761 ctgggactgg cggacatgga cgtggtgctg caactcctcc ggacccgaat cgcgcctgcc
    11821 agcaatcttc ccctgcccgt cttcgagccg gcgcagatca tgcagtaccg ctccggggag
    11881 gagttccggc cgcactacga tttcctcgat ccggatcagc ccggccacgc tccggagctg
    11941 gcgcgcttcg gccagcgcat cgccaccttc ctcctctacc tgaacgacga tttcgagggc
    12001 ggcgagacgg cctttcccaa gaccgggttc aaatatcgcg gccgccgcgg cgacgcgctg
    12061 ctgttcgcca atgtcgatgc ggcgacgcgg gcgcccgacc cgctgacgct ccacgccggc
    12121 ctgccgccga tgcgcgggga gaaatggatc ttctcgcagt ggatccgcga ccggtcgccg
    12181 ggaccgccgg cgggcggcta ggcgtccgcc agggcgaggt tgcgatcggc ccggagcagg
    12241 acgtcctcga cgccgtcgcc gggaaggatt ccggtcgtcg cgaccttcgc ttccagcttc
    12301 acccggacat cgccgaggtc gagcggattg gcggcgatgc agcgtgccag gcgctcggcg
    12361 gtgtcgatcg cgctgttgtg gtccagatga tcgaggatca ggccgaagcg gttgtcgccg
    12421 gtgcgggcga ggacgtcggt cgagcggatc aggccgcaca gcagccgggc gacatggacc
    12481 aaggcggcat cggcggcgaa atggccatgg ctctcgtgga tttcggcgag cgaagtcagc
    12541 tcgatgccga ccaccgcggc gggcgtccgg tgccgctcgg caagcccgac gacgcgctcg
    12601 agctcggcgc ggaagcggcg cgcgctggga agcggggtga cggtgtcgct ctcggcgttg
    12661 cgctcgagct cgacgatgcg ctgctgcgcc tccgccagcg ccgctcggag aagcgcattc
    12721 tcgtcggagg tctcgaccgg caggcccgcg aagtctgatg catgtttcga catgaggaca
    12781 gcctagcgca ctctggttaa gcaaaaacga agctctggcc tccccgcgcc ccgcccgata
    12841 gaggtgcgca ggtcatggcc gaggtcggga tcatcatggg cagccgctcc gactgggaga
    12901 cgatgcgcca tgccgccgac atgctcgacg cgctcgaaat tgcctacgaa acgaaagtcg
    12961 tgtcggccca ccgcacccct gagcggctct acgattatgc gcgcggcgcg gccgggcggg
    13021 gcctcaaggt gatcgtcgcc ggcgccggcg gagcggccca cctgccggga atggcggcgt
    13081 cgatgacccg gctgcccgtg ctcggagtgc cggtccggtc gagagcgctg gacggcctcg
    13141 acagcctgct ttccattgtc cagatgcccg ccggagtgcc cgtgggcacg ctggccatcg
    13201 gccaggccgg cgcctccaat gccggcctgc tcgcggcctc catcctggcc ttgtccgacg
    13261 aggccctggc cggccgcctc gaggcctggc ggcagcggca gagcgacggc gtcgccgaag
    13321 cgccggaatg acgctgcccc c
//
LOCUS       DASVQZ010000047         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_490563, whole genome shotgun sequence.
ACCESSION   DASVQZ010000047 DASVQZ010000000
VERSION     DASVQZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490563"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VF547_01280"
     CDS             complement(<1..1052)
                     /locus_tag="VF547_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8261486.1"
                     /translation="MPEAYIVAAARTAGGRRGGALKDWHPADMAAEVLDALIERSGLD
                     PAAVEDVIMGCVGQAGEQAFHIGRNAVLASSLPTSVPAVTIDRQCGSSQQAVQFAAQA
                     VMSGTQDVVIAAGVESMTRVPMGLPVTLPMQHGIGSGPFSKRIQERFGVTMFSQFTGA
                     EMIAEKYQFRKEQLDAFALESHRKAAAATEAGAFEEEIVLLKVVDAEGKESLHTRDEG
                     IRYDATLEGIASVKLIKDGGKITAANASQICDGASGVVVVSEAALKAHGLTPLARIVN
                     LTVTGGDPVVMLEEVIPATRRALKRSGMSIADIDLYEVNEAFAPVPLAWLREVGADPA
                     RLNVNGGAIALGHPLGA"
     gene            complement(1225..1665)
                     /locus_tag="VF547_01285"
     CDS             complement(1225..1665)
                     /locus_tag="VF547_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261487.1"
                     /translation="MRTGEERRGEREAAGAEVGGAAAASAANLFQVTLVIVPPGGGEA
                     EYSLQVAVPALPREGDYVTVMRRREGPVAGKHIGTEDFIVRRVWWAFDYADDGRLYEE
                     PHDRIIGSVNGIGIECELAKGHYSSEAHRRACGAGARTFEASAY"
     gene            complement(1810..2694)
                     /locus_tag="VF547_01290"
     CDS             complement(1810..2694)
                     /locus_tag="VF547_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261488.1"
                     /translation="MKKLASLSSVLAALPLAACGTMALDEPQSLGERNAAYSYIPLDP
                     LPVFITNRKGCPTASASDPRTLMDSLPDNAVRIAIREIIGKGDVSLGPVAVGTSGSRY
                     QVVLDYINVDTANIRFSVGVKGDEELLPLSTYSEGREYFARRIDDSAVTGPNLVVIPV
                     YVGVGLRLTANVEVLRGKVNLASLGAIAAGAEANRVAGSLVVQTLGINGKQVAATLPL
                     PSELNATTVQQAIQSLGAIKAIMYDTQNTRVVPRVTGIYNPLETDDPKLINLIVSQLA
                     ESHIPWSPPCAGAGTTAD"
BASE COUNT          413 a          956 c          917 g          457 t
ORIGIN      
        1 gcgccgagcg ggtggcccag agcgatggcg ccgccgttga cgttgagccg ggcggggtcg
       61 gcgccgacct cgcgcagcca ggcgaggggg acgggggcga aggcctcgtt gacctcgtag
      121 aggtcgatgt cggcgatgct catgccgctt cgtttcagcg cccggcgggt cgcggggatg
      181 acctcctcga gcatgaccac ggggtcgccg ccggtgacgg tcaggttgac gatccgggcg
      241 agcggggtga ggccgtgcgc cttcagcgcg gcctccgaga cgacgacgac gccggaggcg
      301 ccgtcgcaga tctggctggc gttggccgcg gtgatcttgc cgccgtcctt gatcagcttg
      361 accgacgcga tgccttcgag cgtggcgtcg tagcggatgc cctcgtcgcg cgtatggagg
      421 ctctccttgc cctcggcgtc gaccaccttg agcagcacga tctcctcctc gaacgcgccg
      481 gcctcggtcg cggcggccgc cttgcggtgg ctctccagcg cgaaggcgtc cagctgctcc
      541 ttccggaact ggtatttctc ggcgatcatc tcggcgccgg tgaactggct gaacatcgtc
      601 accccgaacc gctcctggat ccgcttcgag aaggggccgg agccgatgcc gtgctgcatc
      661 ggcagggtga cgggcagccc catcggcacc cgggtcatcg attcgacccc cgcggcgatg
      721 acgacgtcct gggttccgct catcaccgcc tgggcggcga actggaccgc ctgctgcgac
      781 gatccgcact ggcggtcgat ggtgacggcc ggcaccgagg tcggcagcga ggaggcgagg
      841 acggcgttgc ggccgatgtg gaaggcctgc tcgccggcct ggccgacgca gcccatgatc
      901 acgtcctcga ccgccgccgg atcgagcccg gagcgctcga tcagcgcgtc gagcacctcg
      961 gcggccatgt cggccggatg ccagtccttc agcgccccgc ccctgcgccc gccggcggtc
     1021 cgggcggcgg cgacgatata agcttcgggc atggacgggc tcctcgggat ggacggccgc
     1081 cgttagcatc cccggctccg aatggggagg gggccggcgc cggcggtccc ctgcggccga
     1141 cccttccttc ccccggccgc tcccggctgc cgggccgagc tcggatcggc gacgggagcg
     1201 cgtccgcctc gcagagcgac gatctcagta ggcgctcgcc tcgaaggtcc tcgcgccggc
     1261 tccgcaggcc cgccggtggg cctcggacga ataatggccc ttcgccagct cgcactcgat
     1321 gccgatgccg ttgacggagc cgatgatcct gtcgtgcggc tcctcgtaga gccggccgtc
     1381 gtccgcatag tcgaaggccc accacacccg cctgacgatg aagtcctcgg tcccgatatg
     1441 cttgccggcg acgggaccct cgcgccggcg catcaccgtc acataatcgc cctcgcgcgg
     1501 cagcgccggc accgcgacct gaaggctgta ctcggcctcg ccgccgcccg gcggcacgat
     1561 cacgagggtc acctggaaca gattcgccgc gctcgcggcg gcagcgcccc caacctccgc
     1621 cccggccgcc tcgcgctcgc cgcgccgctc ctcccccgtc ctcattctcc cgccctcaaa
     1681 tcgccgagaa acaggaacac tacaggaaca gaaggatcga agcaagcgga attgcgcacg
     1741 ctcttatcga agctcaaagc gctggcccgg atttggaggt tttcggacag cacgccagaa
     1801 atgcgcgcgt cagtccgccg tggtcccggc gccggcgcaa ggcggggacc acggtatgtg
     1861 cgattcggca agttgggaca cgatcaggtt gatcagcttt ggatcgtcgg tttcgagcgg
     1921 attgtagatt cccgtcaccc tcggcacgac ccgggtattc tgggtgtcgt acatgatcgc
     1981 tttgattgcg ccgaggctct ggatcgcctg ctggaccgtg gtggcgttca gctcgctcgg
     2041 aagtggcagc gtcgccgcga cctgtttgcc gttgatgccc agcgtctgca cgacgagcga
     2101 gcccgccacc cggttcgctt cggcccccgc cgcgatcgca ccaaggctgg caaggttcac
     2161 cttcccccga agcacctcga cattcgccgt cagccgcagg cctacgccga catagaccgg
     2221 gatgaccaca aggttggggc cggtgaccgc actgtcgtcg atccgccgcg cgaaatattc
     2281 ccgtccctca ctataggtcg agagcggcag cagctcctca tcgcctttga ctccgaccga
     2341 gaagcggatg ttggccgtgt ccacgttgat atagtcgagc acgacctggt accggctgcc
     2401 gctggtgccg accgccacgg gccctagcga gacgtcgccc ttcccaatga tctcacgaat
     2461 tgcgatgcgc accgcattgt cgggcaggct gtccatgaga gtccgagggt cgctcgccga
     2521 cgcggtcggg catcctttcc gattcgtgat gaagacgggg agggggtcca gcggaatgta
     2581 cgagtaagcg gcgttacgct cgccgagcga ctgcggctcg tccagcgcca tcgttccgca
     2641 ggccgcgagc ggcaaagcgg caagaacgct cgacaggctt gcaagctttt tcatgcctaa
     2701 attcctctcg gttcggcaag tgcttagccg cttcgcaagg cgt
//
LOCUS       DASVQZ010000048         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_299791, whole genome shotgun sequence.
ACCESSION   DASVQZ010000048 DASVQZ010000000
VERSION     DASVQZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299791"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF547_01295"
     CDS             <1..193
                     /locus_tag="VF547_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261489.1"
                     /translation="FGFSSLHPEMGSTIRGLAALAPGTLGLMHGPAFTGDGAAALIAL
                     ADDYDRRTAAWRERRAQAA"
     gene            293..583
                     /locus_tag="VF547_01300"
     CDS             293..583
                     /locus_tag="VF547_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261490.1"
                     /translation="MRLTLALPLLALAACNVDNDSANDQMTVEYNQQRIEDAAAATAR
                     TARDVGSAAGNVAVSTGRAIGNEVGDIDVDVDVSRNRSGQGNRPESNEAEAR"
     gene            complement(605..751)
                     /locus_tag="VF547_01305"
     CDS             complement(605..751)
                     /locus_tag="VF547_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX8261491.1"
                     /translation="MFEDPVAKRTAVAAAIGAVVAIPVPFVGPLFGAVVGAGIGYLSA
                     KRRG"
     gene            937..2349
                     /locus_tag="VF547_01310"
     CDS             937..2349
                     /locus_tag="VF547_01310"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8261492.1"
                     /translation="MGERVFISYAHVDHRALERLHVFLEPLRREGKIITWTDNEVTGG
                     RRFKTEISKALEDSGIFIALVSPDYLHSSYCYDIEFRRAQALEEAGGLTILPVILEPC
                     DWLSTPLGEFKALPRDGAPISTWPNENEAFLDVVKGIRRTLRTPAAATAAAQASPTRA
                     ADPAWSDKRRWRRVAAYVAPERIENPHLSTNRDHRPVVILNAPGRVDCFDVYKGEAET
                     FYEFPDEPPPYADCLVCHGLTSVYARTSGNLGVMTIDEQTGDYESETLKAHSSTPILA
                     DFNHDATRFVSGDATGEVHVWNWPKRKIESTLSTLVEAAVRAAFSLDGRHVAIIGQTG
                     QIELFDLATTRSLARASATVRGKRADAAFSLDNAHLAAFDGADRWDVFSVPGLARVPS
                     EDWREIMIGLRGSAENTLVSSSAAGSLTIMERRAGTSGYERDEIGWPDRRATDLAVSE
                     DGRHVAVILDRRELCVYAKA"
     gene            2465..2929
                     /locus_tag="VF547_01315"
     CDS             2465..2929
                     /locus_tag="VF547_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261493.1"
                     /translation="MRRTAIDLARGFTAALLSTLPGAWLLGSMSNSLGGWILGGLAAL
                     PFVAAGLLLAAPAMLALRRAGRNSALNYLLLGAGSGVLVESMLEVAIAVGTPYSPLQL
                     GGAWGAAVGLWSSAMWCLIFRDRPRSFGRGSAAPGSTPGTGGPAEAAGLLRR"
     gene            2981..3472
                     /locus_tag="VF547_01320"
     CDS             2981..3472
                     /locus_tag="VF547_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261494.1"
                     /translation="MTGRLSASSVGAAAALAVAAAALVLPSQPAKAQLDPNGTGNCRV
                     GGTVSLRDGRVGTVVRAQGNSCHVRLADGSEQYALQWMLTPVRGGKGAPASGAGGKGA
                     PAGGGKLVPGNYQCYGGAAGNMRVRLTGPRYNGAYAVPLPDGRVGLSYRPGGPFYMTC
                     ERR"
BASE COUNT          535 a         1306 c         1229 g          537 t
ORIGIN      
        1 gttcggcttc tcgagcctcc accccgaaat gggctcgacc atccgcggcc tcgccgcgct
       61 cgcgcccggg accctggggc tgatgcacgg gcccgccttc accggcgacg gcgccgcggc
      121 cctgatcgcc ctcgccgacg attacgaccg ccgcaccgcg gcctggcgcg agcggcgcgc
      181 gcaggcggcc tgaacggccg gcgccggctc ggcacccggc gctcgcgcgg ccggctggcg
      241 gaccccgggg ggcttggagg cgttggtcgc ccgattgcaa aggagccccg ccatgcgcct
      301 caccctcgcc ctgcccctgc tcgcgctcgc cgcctgcaac gtcgacaacg attcggccaa
      361 cgaccagatg accgtcgaat acaatcagca gcggatcgag gacgcggccg ccgccaccgc
      421 ccgcaccgcc cgcgacgtcg gctccgccgc cggcaacgtc gcggtcagca ccggccgggc
      481 gatcggcaac gaggtcggcg atatcgacgt cgacgtcgac gtcagccgca atcgctcggg
      541 acagggcaat cggcccgagt cgaacgaggc cgaggcgcgc tagcggccag ccgccgccgc
      601 cggctcagcc gcggcgcttg gccgacagat agcctatgcc ggcgccgacg acggcgccga
      661 acagcgggcc gacgaacggc accgggatcg ccaccaccgc gccgatcgcc gccgcgacgg
      721 cggtccgctt cgccactgga tcctcgaaca tctttctcgc ctccggttga gggtgtgtta
      781 tagcaataat acagccgtcc gtccagcctc ctgatcgcag aggcatcgat attctgccgg
      841 cgcccagagg ccggtgcgca ccggcctgca aagcgcttga gttcgatgct gtcggatgca
      901 acgcctctgt gcatcgtgga ttcgacggag gacaaggtgg gggaacgtgt cttcatctct
      961 tacgcgcacg tggaccatcg ggcgctcgag cggctgcacg tcttcctcga gcctctccgc
     1021 cgcgaaggga agatcatcac ctggaccgac aatgaggtga cgggcggccg ccgcttcaag
     1081 acggagatct cgaaggcgct cgaggacagc ggcatcttca ttgccctggt cagccccgac
     1141 tatctccact cctcctattg ctacgacatc gagttcaggc gggcccaggc gctcgaggag
     1201 gcgggcggcc tgacgatcct cccggtgatc ctggaaccct gcgactggct gtccacgccc
     1261 ctgggagagt tcaaggcgct ccctcgggac ggggcgccga tctccacctg gccgaacgag
     1321 aacgaggcct tcctcgatgt cgtgaaaggt atccggcgga ccctccggac gccggcggcc
     1381 gcgacggcgg cagcccaggc gtctccgacg cgcgcggccg acccggcatg gtcggacaaa
     1441 cgccgatggc ggagggtggc ggcctatgtg gcgcccgagc ggatcgaaaa cccccatttg
     1501 tcgacgaacc gggaccatcg gcccgtcgtc atcctcaacg cgccgggacg ggtggattgc
     1561 ttcgacgtgt acaaaggcga ggcggaaacc ttttacgagt ttcccgacga gccgccgccc
     1621 tatgcggact gcctcgtgtg ccacggcttg acgtcggtct acgcgcgcac ctccggcaat
     1681 ctcggagtca tgaccatcga tgagcagacc ggcgactatg agagcgagac cctcaaggcc
     1741 cattccagca cacccatcct ggctgatttc aatcatgacg ccacgcgctt cgtctccggg
     1801 gacgcgaccg gcgaggtgca tgtctggaac tggccgaagc ggaagatcga atcgaccctg
     1861 tcgactctcg tcgaggcggc ggtcagagcc gcattctccc tggacggacg ccatgtcgcc
     1921 atcatcgggc agacgggtca aatcgagctc ttcgatcttg ccacgacgag gtcgctggcg
     1981 cgggcttcgg cgacggtgcg cggcaagcgg gcggacgcgg ccttcagcct cgacaacgcg
     2041 catctggcgg cattcgacgg cgccgatcga tgggacgtct tctcggtccc gggactggcg
     2101 cgggtgccca gtgaggactg gcgggagatc atgataggcc tgcgcggctc cgcggagaac
     2161 accctggttt cctcctccgc agccggttcg ctgacgatca tggagaggcg ggccggcact
     2221 tcgggctacg agagggacga gatcggctgg cccgaccggc gcgccaccga cctggcggtc
     2281 tcggaggacg gccggcacgt cgccgtcatc ctcgaccgcc gcgagctctg cgtctacgcc
     2341 aaggcctgag cttcgtccgc ccggcggcgc gtccccttcc cgatcgggac gcccgcggcg
     2401 gccagcgtta aggtcctcgt gaaggcttcg gcctagggtc gccccgaacg gggggaaaat
     2461 ccacatgcgc aggacagcga tcgatctcgc caggggcttc accgcggcgc tgctgagcac
     2521 gctccccggg gcctggctgc tcgggagcat gtcgaattcg ctcggaggct ggatcctcgg
     2581 cggactcgcg gcgctgcctt tcgtcgccgc cgggctgctg ctcgcggctc cggcgatgct
     2641 cgcgctccgg cgggcggggc ggaactccgc gctcaactac ctcctgctcg gggcgggctc
     2701 gggcgtgctg gtcgaatcga tgctggaggt cgcgatcgcc gtcggcacgc cctattcgcc
     2761 gctccagctg ggcggcgcct ggggcgccgc cgtcggactg tggagcagcg cgatgtggtg
     2821 cctgatcttc agggaccggc cgcggagctt cggccgcggt tccgccgcgc cgggctcaac
     2881 cccgggaacg ggcggtccgg cggaggcggc cggcctgttg cggcgatagc gatccgatgg
     2941 catttccgcc cgatcgaagg cgttcaagga gaaatccagc atgaccggaa gattgtcggc
     3001 atcgtcggtc ggcgcggccg ccgcgctcgc cgtcgccgcg gcggccctgg tccttccgtc
     3061 ccagcccgcg aaggcgcagc tcgaccccaa cgggaccggc aattgccggg tcggaggcac
     3121 ggtctcgctc cgcgacggcc gggtcggaac cgtcgtccgc gcgcagggca acagctgcca
     3181 cgtccgcctc gccgacggca gcgagcaata tgccctgcaa tggatgctga ccccggtccg
     3241 cggcggcaag ggcgccccgg cgtccggcgc cggcggcaag ggcgctccgg cgggcggggg
     3301 caagctcgtc cccggcaatt accaatgcta tggcggcgcc gccggcaaca tgcgcgtcag
     3361 gctcaccggc ccccgctaca acggcgccta cgccgtcccg ctccccgacg gccgggtcgg
     3421 cctctcctac cgccccggcg gccccttcta catgacctgc gagcgccggt agggcgaagg
     3481 cggccggcct tttttccggt cctcgcgcgg gtccggtcgc gacctcgaaa aggcctctcg
     3541 ccccgccctc cccgatccga cgtccgccgt cggcggcatt tccgaccggc tttcgaaccg
     3601 atctggt
//
LOCUS       DASVQZ010000049         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_11562, whole genome shotgun sequence.
ACCESSION   DASVQZ010000049 DASVQZ010000000
VERSION     DASVQZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11562"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VF547_01325"
     CDS             complement(<1..548)
                     /locus_tag="VF547_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8261495.1"
                     /translation="MATSPIVESASPPYASDSFQRGLARWNRARLVPAFPEDGWPGAE
                     ERDERMRRQEGGFIDSLRAEIAAEAAGAPAEPDAFLAWFEGLRETGPGQKDPLFPWLA
                     ERADREQMRWFLGQEAAGEAGFEDLLALTQVRMPARAKLEFARNYWDEMGRGNPKGMH
                     GPMLDTLIETLRLRPAIETTVW"
     gene            704..1150
                     /locus_tag="VF547_01330"
     CDS             704..1150
                     /locus_tag="VF547_01330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEX8261496.1"
                     /translation="MLMVETELRQSAIHGTGVFLLEPVRKGDLIWRFDSRIDRVYSED
                     DLASLPERMQRFLRTYSTWHEATRLWILCGDNGRHFNHSDTPNTLSLGVGFGDDVAAL
                     DLPAGVELTTNYALICDSVRRNGASYLSAAAPASAASEKRPSAATV"
     gene            complement(1084..2412)
                     /locus_tag="VF547_01335"
     CDS             complement(1084..2412)
                     /locus_tag="VF547_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261497.1"
                     /translation="MRFLGVTETCDLGSLYMRLITEGHEVRVFIEDEAAHGTMAGLVP
                     RTGDWRAELDWVGRDGIILFEAVSEGYGALQDVLRAQGHMVIGGSAFGDRLENDRAYA
                     QALLAGLGLRTAAVHEFDDPAAGDAFLAARPGRYVLKFSGPDFASSDNYVGQRADGAD
                     VRAVLAARFRARGGERASYILMDHVDGVEMGVGAYFDGERFLAPACLDWEHKRFFAGD
                     MGELTGEMGTVATFERSGRFFDLTLARIAPLLREQGHVGYVNLNTIVNAEGIWPLEFT
                     CRFGYPGFAVLEPLQAIGWADLFLAMARRSAAEFPVRPGFSLGVVLTTPPFPYTRRQV
                     EEPVGLPVLLDPALGAEDRRHLHFGEVGLEGGALVTSGLYGWTMVVTGTGPDIAAAKR
                     AAYGRAGRVSIPAVRYRLDIGDRLIAGDHQTVAALGLFSEAADAGAAADR"
     gene            complement(2412..>2677)
                     /locus_tag="VF547_01340"
     CDS             complement(2412..>2677)
                     /locus_tag="VF547_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8261498.1"
                     /translation="GLVNRIEADRDAVVAAGLDLARTIAAKSPLAVAGAKMSLNYSRG
                     RTVEEGLRHVALWNAGALVSADLTAAVQGRLSKQVPEFKDLAD"
BASE COUNT          411 a          949 c          939 g          378 t
ORIGIN      
        1 tcccataccg ttgtctcgat cgccggccgg agcctcagcg tttcgatgag agtgtccagc
       61 atcgggccgt gcatgccttt gggattgccg cggcccatct cgtcccaata gttgcgcgcg
      121 aactcgagct tggcgcgcgc cggcatccgg acctgggtca aagcgagcag gtcctcgaag
      181 cccgcctccc cggccgcttc ctggcccagg aaccagcgca tctgctcgcg gtcggcgcgc
      241 tcggcgagcc aggggaagag cgggtccttt tgtccggggc cggtctcgcg caggccctcg
      301 aaccaggcga ggaaggcgtc gggctcggcc ggcgcgccgg cggcctcggc ggcgatctcg
      361 gcgcgcagcg aatcgatgaa gccgccctcc tgccgccgca tccgctcgtc gcgttcctcg
      421 gcgccgggcc agccgtcctc cggaaaggcc gggaccagcc gggcccggtt ccagcgggcg
      481 aggccgcgct ggaaggagtc gctggcgtag ggcggggagg cggattcgac gatcggtgag
      541 gtagccaagc tcgggccttc gggaaggtgg gcgcgcccgg tcaaccattc cggaagtcca
      601 atgttccgct taacctatat caagcaaata cttacccgga acagcggcaa tcccgacgcg
      661 ttggcggcgc gatcgaacca ctctccggaa gaagaacgcc ttcatgctga tggtagaaac
      721 cgagcttcgc cagagcgcca tccacggcac cggcgtgttc ctgcttgagc cggtgcgcaa
      781 gggcgacctc atctggcggt tcgattcgcg catcgaccgg gtgtacagcg aggacgacct
      841 agccagcctg cccgagcgca tgcagcgctt cctgcgcacc tattcgacct ggcacgaggc
      901 gacccggctg tggatcctgt gcggggacaa cggccgccat ttcaaccatt cggacacgcc
      961 caacacgctc tcgctgggcg tcggcttcgg cgacgacgtc gcggcgctcg acctgccggc
     1021 gggagtcgag ctcaccacca actacgcgct gatctgcgac agcgtccgaa ggaacggggc
     1081 gagctacctg tccgcggcgg cgccggcgtc ggccgcctcg gagaagaggc cgagcgcggc
     1141 gaccgtctga tggtcgccgg cgatcagccg gtcgccgatg tcgagccgat agcggaccgc
     1201 cgggatggag acccgtccgg cgcggccgta ggcggcccgc ttcgccgccg cgatgtccgg
     1261 cccggtcccc gtcaccacca tggtccagcc atagaggccg ctggtgacca gcgccccgcc
     1321 ttcgaggccg acctcgccga aatggaggtg gcgccggtcc tccgcgccga gcgccgggtc
     1381 gaggaggacg ggcaggccga ccggctcctc gacctgcctt cgcgtatagg ggaagggcgg
     1441 ggtggtcagg acgacgccga ggctgaagcc gggccggacc gggaattcgg cggcggaccg
     1501 ccgcgccatc gccaggaaaa ggtccgccca gccgatcgcc tggagcggct ccagcaccgc
     1561 gaagccggga tagccgaacc ggcaggtgaa ctcgagcggc cagatgccct cggcgttgac
     1621 gatcgtgttg aggttgacgt agccgacatg gccctgctcg cgaagcagcg gcgcgatccg
     1681 cgccagggtc aggtcgaaga accggccgct gcgctcgaag gtcgcgacgg tgcccatctc
     1741 gccggtcagc tcgcccatgt cgccggcgaa gaagcgcttg tgctcccagt cgaggcaggc
     1801 cggggcgagg aaccgctcgc cgtcgaaata ggcgccgacc cccatttcga cgccgtcgac
     1861 atggtccatc aatatgtagc tcgcacgctc cccgccgcgc gcccggaagc gtgccgccag
     1921 caccgctcgg acgtcggcgc cgtcggcgcg ctggccgaca taattgtcgc tcgacgcgaa
     1981 atccggcccg ctgaacttga ggacgtagcg gcccggccgg gcggcgagga aggcgtcccc
     2041 cgccgccggg tcgtcgaact cgtgcaccgc ggcggtcctc agcccgagcc cggcgagcag
     2101 cgcctgggca taggcgcggt cgttctcgag ccggtcgccg aaggcgctgc cgccgatcac
     2161 catgtggccc tgcgcgcgaa gcacgtcctg aagcgcccca tagccctccg acacggcctc
     2221 gaagaggatg attccgtccc ggccgaccca gtccagctcg gcccgccagt cgcccgtccg
     2281 ggggacgagg ccggccatgg tcccgtgcgc ggcctcgtcc tcgatgaaga ccctgacctc
     2341 gtgcccctcg gtgatgaggc gcatgtagag gctgcccagg tcgcaggtct cggtcacccc
     2401 gaggaagcgc atcagtcggc gaggtccttg aactcgggga cctgcttgct cagccggccc
     2461 tggacggcgg cggtgagatc ggcgctgacc agcgccccgg cattccacag cgcgacgtgg
     2521 cgcaggccct cctccaccgt ccggccgcgg ctgtagttga ggctcatctt ggcgccggcg
     2581 acggcgagcg gggacttggc ggcgatggtc cgcgccagat cgaggccggc ggcgacgacc
     2641 gcgtcccgat cggcctcgat gcggttgacc aggccga
//
LOCUS       DASVQZ010000050         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_685188, whole genome shotgun sequence.
ACCESSION   DASVQZ010000050 DASVQZ010000000
VERSION     DASVQZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685188"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="VF547_01345"
     CDS             <1..1663
                     /locus_tag="VF547_01345"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX8261499.1"
                     /translation="EDGAITYMRTDGVQMAPEAVSGARRAVTERFDAGYVPDKPRAYQ
                     TKAKNAQEAHEAIRPTDFGRDRAGSGDHGRLYELIFKRALASQMASARLERTTAELSD
                     GTGQNGLRATGQVVLFPGFLALYEEGRDEPDDDESRRLPRLREGDAPAKKKVVAEQHF
                     TQPPPRYSEASLVKKMEELGIGRPSTYAATLQTLKDRSYVELEKNRFVPLESGRLLTA
                     FLERFFERYVSFDFTAHLEDELDDISGGRAQWQQVLEAFWRDFKPKTAEIMEQQPSAI
                     TAALDDFLAPWLFPDRQDGTDPRLCPACGEGRLSLRGGRFGAFVACSNYPECRFTRRF
                     GQGGEEGGSDSGPAVLGTDPESGEEVSLRTGRFGAYVQLGEGKDSKRASIPRDVAQDQ
                     VDLAQALRLLSLPRTIGTHPESGKPITASIGRYGPYLAHEGKYAKLGSTAEVFETGMN
                     AAVVKLAEAAAGAGRARGSREPLKRLGAHPRTEAEIRLMEGRFGPYVTDGTTNATLPK
                     SLDPDQLTLEEAAQLIDERAAKGPPVKKSRGRKPAARKAPAKKKG"
     gene            1663..1959
                     /locus_tag="VF547_01350"
     CDS             1663..1959
                     /locus_tag="VF547_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261500.1"
                     /translation="MRRRWTLVGIGVVAELVALALWAFVGSYQGLFWPFVLLSVLAAL
                     AGAWIVLATMIDITFHRPRGERLRPLRGFDLVLGGGLFVLGLLQLRDALGQLPA"
     gene            complement(1923..2711)
                     /locus_tag="VF547_01355"
     CDS             complement(1923..2711)
                     /locus_tag="VF547_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX8261501.1"
                     /translation="MTLGDDQLDRYARHIVLREIGGEGQVRLLAAHVALVGAGGIGGP
                     ALQYLAAAGIGRLTVIDDDRVSLDNLQRQILFGTADVGRAKAEVAEEAAARLNPDVRF
                     SGRRERLTAANAGALLAGADVVVDGSDNFATRLVVSDTCTELRIPLVSAAIGQFQIQV
                     GTWRGWEADRPCYRCFVGDAFDSEDCDSCAELGVLGAAAGMAGSWAALEAIRQIVGFG
                     GEPAGRVHVFDGLAPAMRSLKVTKDPACRACADQAGSWPSASRS"
     gene            complement(2708..3145)
                     /gene="dut"
                     /locus_tag="VF547_01360"
     CDS             complement(2708..3145)
                     /gene="dut"
                     /locus_tag="VF547_01360"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX8261502.1"
                     /translation="MIDIEVKRLSNGEGLPLPSYASDGAAGLDVVAAEDLTLAPGARR
                     AVATGFAIAIPKGFEVQVRPRSGLALKHGITCLNTPGTIDSDYRGEVKVILANLGAEP
                     FEVRRGERIAQLVPAAVLQAAFREVEALDSTERGAGGFGSTGR"
BASE COUNT          484 a         1185 c         1150 g          442 t
ORIGIN      
        1 cgaggatggg gcgatcacct atatgcggac cgacggcgtg cagatggcgc cggaggcggt
       61 ctccggagcg cggcgtgccg tcaccgagcg gttcgacgcc ggctacgtgc cggacaagcc
      121 gcgggcctac cagaccaagg ccaagaacgc ccaggaagcg cacgaggcga tccgcccgac
      181 cgatttcggc cgcgaccggg ccggctcggg cgaccatggc cggctctacg agctgatctt
      241 caagcgcgcc ctggccagcc agatggcctc ggcgaggctc gagcggacca ccgccgagct
      301 cagcgacggc accggccaga acgggcttcg cgcgaccggc caggtcgtcc tcttccccgg
      361 cttcctcgcc ctctacgagg aaggccgcga cgagcccgac gacgacgaat cccgccgcct
      421 gccgcggctg cgcgagggcg acgccccggc gaagaagaag gtcgtcgccg agcagcattt
      481 cacccagccg ccgccccgct attccgaggc gagcctggtc aagaagatgg aggagctcgg
      541 catcggccgc ccgtcgacct acgcggcgac cctgcagacc ctcaaggacc gatcctacgt
      601 cgagctcgag aagaaccgat tcgtgccgct ggagagcggg cggctcctca ccgccttcct
      661 cgagcggttc ttcgagcgct acgtctcgtt cgacttcacc gcccatctcg aggacgagct
      721 cgacgacatc agcggcggcc gggcgcagtg gcagcaggtg ctcgaggcgt tctggcgcga
      781 cttcaagccg aagaccgccg agatcatgga gcagcagccc tcggcgatca ccgccgcgct
      841 cgacgatttc ctcgcgccct ggctgttccc cgacaggcag gacggcaccg acccgcgcct
      901 ctgccccgcc tgcggcgagg ggcggctgtc gctgcgcggc ggccggttcg gcgccttcgt
      961 cgcctgctcc aactatcccg agtgcaggtt cacccgccgc ttcggccagg gcggcgagga
     1021 gggcgggtcg gattccgggc cggcggtgct cggcaccgac ccggaatcgg gcgaggaggt
     1081 gagcctgcgc acggggcggt tcggggccta tgtccagctc ggcgagggca aggattcgaa
     1141 gcgcgcgtcg atcccgcgcg acgttgcgca ggaccaggtc gacctcgctc aggcgctccg
     1201 actgctgtcg ctgcccagga cgatcggaac ccatcccgag agcggcaagc cgatcaccgc
     1261 ctcgatcggc cgctacgggc cctatctcgc ccatgagggc aaatatgcga agctcggctc
     1321 gaccgccgag gtcttcgaga ccggaatgaa cgcggcggtg gtcaagctcg ccgaggcggc
     1381 ggccggcgcc ggccgggcgc ggggctcgcg cgagccgctg aagcggctcg gcgcccatcc
     1441 gcgcaccgag gcggagatcc ggctgatgga gggccgcttc ggaccctatg tcaccgacgg
     1501 aaccaccaac gccaccttgc ccaagtcgct cgatccggac cagctcacgc tcgaggaggc
     1561 cgcgcagctg atcgacgagc gcgccgccaa gggcccgccg gtcaagaaga gcaggggccg
     1621 caagccggcc gcgaggaagg ccccggcgaa gaagaagggc tgatgcggcg gcgctggacc
     1681 ttggtcggga tcggagtggt cgccgagctg gtggcgctgg cgctctgggc gttcgtcggg
     1741 tcctatcagg gcctgttctg gccgtttgtg ctgctgtccg tcctcgccgc cttggcgggc
     1801 gcctggatcg tcctcgccac gatgatcgac atcaccttcc accgcccgcg cggcgagcgg
     1861 cttcggccgc tccgcggctt cgacctggtg ctcggcggag ggctgttcgt cctcggcctg
     1921 cttcagctgc gcgacgcgct cggccagctt cccgcctgat cggcacaggc ccggcaggcg
     1981 gggtccttcg tcaccttgag gctgcgcatc gccggcgcca gcccgtcgaa gacgtggacc
     2041 cggcccgccg gctcgccgcc gaagccgacg atctgccgga tcgcctccag cgccgcccag
     2101 ctccccgcca tccccgccgc ggcgccgagc acgccgagct cggcgcagct gtcgcaatcc
     2161 tccgagtcga acgcgtcgcc gacgaagcag cgatagcagg gcctgtccgc ctcccacccg
     2221 cgccaggtgc cgacctggat ctggaactgg ccgatcgccg cggagacgag cgggatccga
     2281 agctctgtgc aggtgtcgga gacgaccagg cgggtggcga aattgtcgct gccgtcgacg
     2341 acgacgtccg cccccgccag caacgccccg gcattggccg cggtgagccg ctcgcgccgg
     2401 ccggagaatc gaacgtcggg attgagcctc gccgcggcct cctccgccac ctcggccttg
     2461 gcgcgtccga cgtcggcggt tccgaacagg atctggcgct ggaggttgtc gagcgagacg
     2521 cggtcgtcgt cgatgacggt cagccgcccg atccccgccg cggcgagata ctgcagcgcc
     2581 ggcccgccga tcccgccggc gccgaccagc gcgacatggg cggcgagcag ccggacctgg
     2641 ccctcgcccc ctatttcccg caggactatg tggcgggcgt agcggtcgag ctggtcgtcg
     2701 ccgagcgtca ccgtccggtc gagccgaatc cgccggcgcc gcgctcggtg gagtcgagcg
     2761 cctcgacctc gcggaacgcc gcctggagca cggccgccgg caccagctgg gcgatgcgct
     2821 cgccgcggcg gacctcgaac ggctcggcgc cgagattggc gaggatcacc ttgacctcgc
     2881 cgcgataatc gctgtcgatc gttcccggcg tgttgaggca ggtgatgccg tgcttcagcg
     2941 ccaggcccga gcgcggccgc acctggacct cgaagccctt ggggatggcg atcgcgaagc
     3001 cggtcgccac cgcccggcgc gcgccggggg cgagcgtcag atcctcggcc gcgacgacgt
     3061 cgaggccggc ggcgccgtcg ctggcatagg aggggagggg aagcccttca ccgttggaaa
     3121 gccgttttac ctcgatgtcg atcatggcgc ctctcgatcc ggtgatcgag cgcgtagcgc
     3181 ccggctccct gggttgcaaa cagcagtccg aggaagacca ggaccagggc gggccaccag
     3241 cccgggaaat cctgcggcca g
//
LOCUS       DASVQZ010000051         8465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_109141, whole genome shotgun sequence.
ACCESSION   DASVQZ010000051 DASVQZ010000000
VERSION     DASVQZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8465
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109141"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..910)
                     /locus_tag="VF547_01365"
     CDS             complement(38..910)
                     /locus_tag="VF547_01365"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX8261503.1"
                     /translation="MRRLSRLVGLAPLLAASPASAHGGDAGAGWTTSPLVTVPLVLAA
                     LVYAVGLMRLWARSGQGRKALRRDCALFAAGWLTLAGALTSPLHRAGATSFTMHMIEH
                     EILMLVSALLLVAGRPGAVLLWALPAELRRPLGSVGRWRLWRVAADPFVATFVQSAVV
                     VAWHVPALFDLALAGDAWHAAQHLSFVLSALLFWWAMLHGRAGPLVSAACLFLSAMIG
                     GGVGGLMALSSSPWYRGYAALGLTPSGLTAGQDQQLAGLIMWVPGGAWHLAAALWFLM
                     RALRNMEARHALPR"
     gene            complement(898..1230)
                     /locus_tag="VF547_01370"
     CDS             complement(898..1230)
                     /locus_tag="VF547_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261504.1"
                     /translation="MTGRRRPLEHLLPWLGMAAAAVGWGASHQVASNAVFDDCRVGDP
                     AFVLLVCLGGLAVAALGGYFSFDVWRRDESEGRRFIGLVGALLAALAAFAILLQAASS
                     LILPPCAA"
     gene            complement(1227..1841)
                     /locus_tag="VF547_01375"
     CDS             complement(1227..1841)
                     /locus_tag="VF547_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX8261505.1"
                     /translation="MREPRFTADLSSLPTHGFGHRALTWWGEIAFFLIEGTAFVMAIA
                     AYFFLMNQEQLWPPPPFAPPDLLAGTLFTLLILLSEIPNSLAKHAAEHGELRKLRRLL
                     LLHSAIGLALLGLRLWEFDSLNVLWSDNAYGSIVWALLLLHAVHFLTDWVDTIVLAAL
                     MHTRHGIEARRFVDCAENAMYWRFVWLSWLPLYGLIYWLPRLVS"
     gene            complement(1841..3772)
                     /gene="ctaD"
                     /locus_tag="VF547_01380"
     CDS             complement(1841..3772)
                     /gene="ctaD"
                     /locus_tag="VF547_01380"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX8261506.1"
                     /translation="MTARMAPGDHDRDGPELTGEALHARLDKTWRREPGLWGWLSTTD
                     HKEIGRRYLVTAFVFLVLGGILALLIRAQLATPESGLIGPDRYNQIFTMHGTTMMFLF
                     AVPVMEAMAVFLIPLMLGTRNIAFPRLNAFSYYVYLAGGCLLWVAFVLDVGPDAGWFS
                     YVPLAGPEFSPGKRVDVWAQMVTFTEVAALAVAVELTATILKHRAPGMNLGRMPLFAW
                     AILIVSLMVIFSMPSVTLASSFLLSDRLVGTHFFNSYELGDSLLWQHLFWFFGHPEVY
                     IIFLPAAGFVSEIVQTFSRRPTFGYTAIVLALAGTGLLAFGLWVHHMFATGLPRLGFA
                     FYTAASMAVSVPAGIQIFCWIATIWAGRPRFATPLLWVIGFVVTFVIGGLTGVMLASV
                     PIDLQLHDTFFVVAHFHYVLIGGAVFPLLGAITYWFPKFTGRMMSERVGKLGFWLSFI
                     GFQVAFFPMHLLGILGMPRRVYTYPAGLGWETLNLVSTIGAFAFALSLLIFVGNALVS
                     LRRGREAGPDPWDAPGLEWATESPPAPYNFAHIPIVSGRTPLWAEGEALPVAHGLRVD
                     DRELLLTTAIDAKPDLREPSAEPTIWPLIAALATTAMFVSSMFTPWAVLVGSVPVAAA
                     LIAWFWPKDPDVHPEPVID"
     gene            complement(3769..4719)
                     /gene="coxB"
                     /locus_tag="VF547_01385"
     CDS             complement(3769..4719)
                     /gene="coxB"
                     /locus_tag="VF547_01385"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX8261507.1"
                     /translation="MTGWLDTLQSAAGKDGQQSAFVWELFAIFLTVTALFYLAVILFL
                     LWAIVRRRRGEEPRGDRGLRTALVGWVGLIALTLAGLTLATWVYDRDLARAADAPALE
                     IEVTARQWWWDVRYADDDADRIFRTANELHLPAGIPVHITLRSNDVIHSFWIPNLAGK
                     QDMIPGRVSDLSIRPLREGLFRAQCAEFCGLQHARMALDVTVESPAAFAAWREAQLRP
                     AAAPAAALEAAGHALVTGRQCASCHMIAGTSAAGQVAPDLTHFASRRTIAAGTLEMNR
                     ANIAAWIADPQRPKPGNNMPKVPLSPAELDAVVAYLETLR"
     gene            complement(4716..5225)
                     /locus_tag="VF547_01390"
     CDS             complement(4716..5225)
                     /locus_tag="VF547_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8261508.1"
                     /translation="MSSPFRAEAALLALLALAALAGCRRETRDYQSEALKAAERGAAF
                     EGNAFHIAQGQRLYAWMNCAGCHAHGGGGMGPPLRDDEWRYGGEIGDIAATILDGRPN
                     GMPAFRARVTEDQAWQLAAFVRALSAQPRQDALPGRADEPRNTEPMTLDERKPIRKVT
                     AKQDDASEE"
     gene            complement(5203..7080)
                     /locus_tag="VF547_01395"
     CDS             complement(5203..7080)
                     /locus_tag="VF547_01395"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX8261509.1"
                     /translation="MLKRLGVWTIFALLLAIAVAAIALSWGMRPSPSERRVAVLETLA
                     PLPRSEARAVLAPSARPAGDGQWTMPLGDYAASRFSTLDEINPANVARLRPVFTFDTG
                     VAKGHEAAPLVIGTTMYIVTPFPNIVYALDLARPGNPLKWVFRPKPLQRAQGVACCDV
                     VNRGAAFADGRLFLNTLDGQTIALDPQTGREMWRTRLANIDIGETITMAPLVAEGRVL
                     VGSSGGEFGVRGWLAALDAASGALLWKAWSTGPDSDVLIGPEFKPFYASERGRDLGVS
                     SWQPEGWKLGGGNAWGWISYDPENHLVYYGTGNPGPWNPDQRPGDNKWTAGLFARDIR
                     TGQARWFYQSSPHDLFDHDDINESILLDLPVGGRRRQVLVRPGRTGFMYVMDRATGQV
                     LSADPYAHVNSARGVDLATGRLIPVPEKAPRSGRVVRDICPASPGGKDWNPSAFSPRT
                     GLIYVPHLNLCMDHGITEANYIAGTPYLGAESRLHAGPGGNRGFLTAWDPVARRAVWR
                     IREDLPLWSGALATAGDLVFYGTMDGLFKAVDARSGRLLWQHRLNSGTIGQPIAYRGP
                     DGRQYVAILAGVGGWSGAIVSGALDKGDPTSALGFVNAVRDLPARTDAGGRLYVFALP
                     R"
     gene            complement(7158..8192)
                     /locus_tag="VF547_01400"
     CDS             complement(7158..8192)
                     /locus_tag="VF547_01400"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8261510.1"
                     /translation="MHLRVAGLSLSVILAACRAETAPPAVRVYVTNEMSGDLTIIDPE
                     ARRVVGRVALGKRPRGIVASPDGRLLYIALSGSPVAGPGVDESTLPAADKAADGIAVF
                     DIASGRVLRVLRGISDPETVAVSPDGNSLYVASEDSGRLIVMDSRSGRIRAAAAVGGE
                     AEGTAVSPDGSLVYATSEEDHTVAVYETATGRVRTRIPVGERPRSIAFAPDGSRAFIP
                     GERDSSVTAIDVATDRVAGKVRVAGENVRPMGIALSPDGRHVFVTTGRGRELVRIVAA
                     SLAVSGRTEVGERPWGLALSPDGRFAFTANGPSNDVAMVDTQSLSVIARFPAGERPWG
                     AAAVRLADPG"
BASE COUNT         1436 a         2792 c         3098 g         1139 t
ORIGIN      
        1 gcctccgcag gccgcaagca gcagcgcgaa ggcgaggcta gcggggcagc gcatgccgag
       61 cctccatatt cctcagcgcg cgcatcagga accacaaggc ggcggcgagg tgccaggcgc
      121 ctccgggaac ccacatgatc agcccggcaa gctgctggtc ctgcccggcc gtgaggccgg
      181 acggagtaag gcccagcgcc gcatagcccc ggtaccaggg gctgctcgac agcgccatca
      241 ggccgccgac gccgccgccg atcatggcgg agaggaacag gcaggcggcg gacaccagag
      301 gtccggcgcg gccatggagc atcgcccacc agaacagcag ggcgctgagg acgaagctca
      361 gatgctgcgc ggcgtgccag gcgtcgccgg ccagcgcgag atcgaaaagc gcgggcacat
      421 gccaggcgac gacgacggcg ctctggacga aggtcgcgac gaacggatcg gccgccaccc
      481 gccacagccg ccaacgcccc acggatccga gcggccggcg gagctcggcc ggaagggccc
      541 agagcagcac cgctccggga cgcccggcga ccagcagcag cgccgagacc agcatgagga
      601 tctcatgttc gatcatgtgc atcgtgaagc tggtcgcgcc ggctcggtgc agcggcgagg
      661 tcagtgcgcc ggccagcgtc agccaccccg ccgcgaacag cgcgcagtcg cgccggagcg
      721 ccttgcggcc ctgcccggag cgggcccaga gccgcatcag cccgacggcg tagaccaagg
      781 cggccagaac gagaggaacg gtgaccagcg gcgacgtcgt ccagccggcg ccggcgtcac
      841 cgccgtgggc cgaggccggg gaggcggcga gcagcggggc gagcccgacc aggcggctca
      901 ggcggcgcat ggcggcagga tcagcgacga cgcggcctgg agcaggatcg cgaaggccgc
      961 cagggcggcg agcagggcgc cgacgaggcc gatgaaacgg cggccctcgc tctcgtcgcg
     1021 gcgccagaca tcgaacgaaa aatagccgcc gagagcggcc acggccagac cgccgaggca
     1081 gacgagcagg acgaaagcgg ggtcaccgac gcggcagtcg tcgaagacgg cgttgcttgc
     1141 gacctgatgg gaggcgcccc agccgacggc cgccgccgcc atgccgagcc aggggagcag
     1201 atgctcgagc gggcgccggc gaccggtcac gagaccagcc tcggcagcca gtagatgagc
     1261 ccgtagaggg gcagccagct cagccagacg aagcgccagt acatggcgtt ctccgcgcaa
     1321 tcgacgaagc ggcgggcttc gatgccgtgc ctggtatgca tcagggcggc gaggacgatc
     1381 gtgtccaccc agtcggtcag gaaatggacc gcgtggagca agagcagggc ccagacgatc
     1441 gagccgtagg cattgtcgct ccagagcacg ttgagggaat cgaactccca gaggcgcaat
     1501 ccgagcaggg ccaggccgat cgcggagtgg agcagaagca gccggcgcag cttgcgcagc
     1561 tcgccatgct cggcggcatg cttggccagg ctgttcggta tctcgctgag caggatcaat
     1621 agggtgaaca gcgtgccggc gagcagatcg ggcggagcaa agggcggcgg cggccacaat
     1681 tgctcctggt tcatcaggaa gaaataggcg gcgatcgcca tgacgaaggc ggtgccctcg
     1741 atcaggaaga aggcgatctc gccccaccag gtcagcgcgc ggtggccgaa gccgtgggtc
     1801 ggcaggctgg acagatcggc ggtgaaacgg ggctcgcgca tcagtcgatc accggctcgg
     1861 ggtggacgtc ggggtctttc ggccagaacc aggcgatcag cgccgcggcg accggcacgg
     1921 agccgacgag gaccgcccag ggcgtgaaca tggacgagac gaacatggcc gtggtggcga
     1981 gcgccgcgat cagcggccag atcgtcggtt cggcggaagg ctcgcgaaga tcgggcttcg
     2041 cgtcgatcgc cgtcgtcagg agcagctcgc gatcgtcgac cctaaggccg tgcgcgaccg
     2101 gcagggcctc gccctccgcc cagaggggcg tgcggccgct gacgatgggg atgtgggcga
     2161 aattgtacgg cgcgggcggc gactcggtcg cccattcgag gcctggagcg tcccacggat
     2221 cgggcccggc ctcgcggccg cggcgcagcg acaccaaggc gttgccgacg aagatgagga
     2281 ggctgagcgc gaaggcgaag gcgccgatgg tggagaccag gttcagggtc tcccagccga
     2341 gcccggccgg ataggtgtag acccggcggg gcatgcccag gatgcccagg aggtgcatcg
     2401 ggaagaaggc gacctggaaa ccgatgaagg agagccagaa gccgagcttg cccacccgtt
     2461 cggacatcat ccgcccggtg aacttgggga accagtaggt gatcgccccg agcagcggga
     2521 agacggcacc gccgatcagc acgtagtgga agtgggcgac gacgaagaaa gtgtcgtgga
     2581 gctggaggtc gatcgggacg ctggccagca tcactccggt gaggccgccg atgacgaagg
     2641 tgacgacgaa gccgatcacc cagagcagcg gcgtcgcgaa gcgggggcgg ccggcccaga
     2701 tcgtcgcaat ccagcagaag atctggattc ccgcggggac cgaaaccgcc atgctggcgg
     2761 cggtgtagaa ggcgaacccc agccggggca gcccggtcgc gaacatgtga tggacccaca
     2821 ggccgaaggc gagcaggccg gtgccggcca gagcgagcac gatcgcggtg tagccgaagg
     2881 tagggcggcg gctgaacgtc tggacgatct cgctgacgaa gccggccgcc ggcaggaaga
     2941 tgatgtagac ctcgggatgg ccgaagaacc agaagaggtg ctgccagagc agcgaatcgc
     3001 cgagctcgta gctgttgaag aaatgcgttc cgacgaggcg atcggagagc aggaagctcg
     3061 acgccagggt caccgacggc atcgagaaga tgaccatcag cgagacgatc aggatcgccc
     3121 aggcgaagag cggcatgcgt ccgaggttca ttcctggcgc gcggtgcttg aggatggtgg
     3181 cggtgagctc gaccgccacg gcgagcgcgg cgacttcggt gaaggtcacc atctgcgccc
     3241 agacgtcgac ccgcttgccc ggcgagaatt ccgggccggc gagcggcacg taggagaacc
     3301 agcccgcgtc cggcccgacg tcgagcacga acgccaccca gagcaggcag ccgccggcca
     3361 gatagacgta gtaggagaag gcgttgagcc gcggaaaggc gatgttgcgg gtgccgagca
     3421 tcagcgggat gaggaacacc gccatggcct ccatcaccgg cacggcgaag aggaacatca
     3481 tcgtggtgcc gtgcatggtg aagatctggt tgtagcggtc ggggccgatc aggccgctct
     3541 ccggagtcgc gagctgggct cggatcagca gagcgagaat cccgccgagg accaggaaga
     3601 cgaaggcggt gacgaggtag cggcggccga tctccttgtg gtcggtggtg ctcagccagc
     3661 cccacaggcc cggctcgcgc ctccaggtct tgtccagccg ggcgtggagc gcctcgccgg
     3721 tcagctccgg gccgtcgcgg tcgtggtcgc cgggagccat tctggcggtc atcgcaacgt
     3781 ctccagatag gcgacgaccg cgtcgagctc ggcagggctg agcgggacct tgggcatgtt
     3841 gttgccgggc tttggtcgct gcgggtcggc gatccaggcg gcgatgttcg cccggttcat
     3901 ctcaagggtg ccggcggcga tggtgcggcg gctggcgaaa tgggtgagat cgggggcgac
     3961 ctggcccgcc gcggaggtgc ccgcgatcat gtggcatgac gcgcattggc ggccggtgac
     4021 gagggcgtgg ccggcggcct cgagcgccgc ggccggcgcc gcagccggcc gaagctgggc
     4081 ttcgcgccag gcggcgaagg cggcgggcga ctccaccgtc acgtcgagcg ccattcgggc
     4141 atgctgcagc ccgcagaact cggcgcactg ggctcggaac agaccttcgc gcagcggccg
     4201 gatcgacagg tcggagaccc ggcccgggat catgtcctgc ttgccggcga gattggggat
     4261 ccagaagctg tggatgacgt cgttcgaacg caaggtgatg tggacgggga tcccggccgg
     4321 aaggtggagc tcgttggcgg tgcggaagat ccggtcggcg tcgtcgtcgg catagcgcac
     4381 gtcccaccac cattggcggg cggtcacctc gatctccagc gccggcgcgt cggcggcgcg
     4441 ggcgaggtcg cggtcgtaga cccaggtcgc cagcgtcagg ccggcaagag tgagcgcgat
     4501 cagtccgacc cagccgacca gcgcggtccg caatccgcgg tcgccgcggg gctcctcgcc
     4561 gcggcggcgt cggacgatcg cccagagcag gaagaggatg acggcgaggt agaacagggc
     4621 ggtgacggtg aggaagatgg cgaacagctc ccagacgaac gcgctttgct gcccgtcctt
     4681 gccggcggcg gactggaggg tgtcgagcca gcccgtcatt cctcgctcgc gtcgtcctgc
     4741 ttggccgtca ccttccggat cggcttgcgc tcgtcgaggg tcatcggctc ggtgttgcgc
     4801 ggctcgtcgg ctcggcccgg aagcgcgtcc tggcgcggct gggcggaaag ggcgcggacg
     4861 aaggcggcga gctgccaggc ctggtcctcg gtcactcggg cccggaacgc cggcatgccg
     4921 ttggggcgcc cgtcgaggat ggtggcggcg atgtcgccga tctcgccgcc gtagcgccat
     4981 tcgtcgtccc tcagcggcgg ccccatgccg ccgccgccgt gggcgtggca gcccgcgcag
     5041 ttcatccacg cgtagagccg ctggccctgg gcgatatgga aggcgttgcc ctcgaacgcg
     5101 gcgccgcgct cggcggcctt cagggcttcg ctctgatagt ccctcgtctc gcggcgacag
     5161 ccggcgagcg cggccagcgc cagcagcgcc agcagcgccg cctcagcgcg gaagggcgaa
     5221 gacataaagc ctccctccgg cgtcggtccg cgccggcagg tcgcggacgg cgttgacgaa
     5281 tcccagcgcc gaggtcggat cgcccttgtc gagcgcgccc gagacgatcg cccccgacca
     5341 gccgccgacg cccgcgagaa tggcgacata ttggcggccg tcggggcctc gataggcgat
     5401 cggctggccg atcgttcccg agttcagccg gtgctgccaa aggagccgcc cgctgcgcgc
     5461 gtcgaccgct ttgaacaggc cgtccatggt cccgtagaag acgagatcgc cggcggtcgc
     5521 gagcgcgccg ctccatagcg gcaggtcctc gcggatccgc cagacggctc ggcgcgcgac
     5581 cgggtcccat gcggtgagaa agccgcgatt gccccccggt ccggcgtgca gccgcgactc
     5641 cgcgcccaga tagggcgtgc cggcgatgta attcgcctcg gtgatgccgt ggtccatgca
     5701 tagattgagg tgcggcacgt agatgaggcc ggtgcggggc gagaaggccg aaggattcca
     5761 gtccttgccg ccgggggagg cggggcagat gtcgcgaacg acccggccgc tgcgcggcgc
     5821 cttctccgga accgggatca gccgccccgt cgccaggtcg acgccgcggg cgctgttcac
     5881 atgggcatag gggtcggccg acaacacctg cccggtcgcc cggtccatga catacatgaa
     5941 gccggtgcgt ccgggccgga cgagcacttg ccgccggcgg ccgccgaccg gcaggtcgag
     6001 caggatgctc tcgttgatgt cgtcatggtc gaacaggtcg tgcgggctcg actggtagaa
     6061 ccatcgcgcc tggccggtgc ggatgtcgcg ggcgaagagg ccggcggtcc atttgttgtc
     6121 gcccggccgc tggtccggat tccagggccc gggattgccg gtcccgtaat agacgaggtg
     6181 gttttcggga tcgtagctga tccagcccca ggcattgccg ccgccgagct tccacccttc
     6241 cggctgccag ctcgagactc cgaggtcgcg gccgcgctcg ctcgcgtaga agggcttgaa
     6301 ttcggggccg atcagaacgt cggagtcggg cccggtgctc cacgccttcc acaacagcgc
     6361 cccgcttgcc gcgtcgagcg cggccagcca gccgcgaacg ccgaactcgc cgcccgagct
     6421 gccgacgagg acccggcctt cggcgaccag cggcgccatg gtgatcgtct cgccgatgtc
     6481 gatattggcg agcctcgtcc gccacatttc ccgtcccgtc tgcgggtcga gcgcgatcgt
     6541 ctggccgtcg agcgtgttta ggaagagccg tccatccgca aacgcggcgc ctcggttcac
     6601 gacgtcgcag caggcgactc cctgggcgcg ctgcagaggc ttcggccgga acacccattt
     6661 cagtggattg ccggggcgcg cgaggtcgag cgcatagacg atgttgggga agggcgtgac
     6721 gatgtacatc gtcgtcccga tcaccagcgg cgccgcctca tgtcccttgg cgactccggt
     6781 gtcgaaggtg aagacgggcc gaagccgcgc cacgttggcc gggttgatct cgtcgagcgt
     6841 gctgaatcgg ctggcggcgt aatcgcccag gggcatcgtc cactgtccgt ctccggcggg
     6901 cctcgccgag ggcgccagga cggcgcgcgc ctcggaccgg gggaggggcg ccagcgtctc
     6961 gagcacggcc acgcgccgct cggacggcga cggcctcatc ccccaggaaa gggcgatcgc
     7021 ggcaaccgcg atcgctaaca gcaaagcgaa gatggtccag acgccgagcc gcttcagcat
     7081 cgccctagtg ggtctcctgc cgtcctcggg cttgaaaatg gcatcgggag gcgaaagttc
     7141 cgcctccgta tcgatttcta acctggatca gccaatcgga ccgccgccgc gccccaaggc
     7201 cgctcgcctg ccgggaaacg cgcgatcacg ctcaggctct gcgtatcgac catcgccacg
     7261 tcgttcgacg gtccgttggc ggtgaaggcg aagcggccgt cagggctgag cgcgaggccc
     7321 cacggccgct ctccgacctc ggtgcgtccg ctgaccgcga ggctcgcggc cacgatccgg
     7381 acgagctcgc gtccccgccc ggtcgtcacg aagacgtgcc ggccatcggg gctcagagcg
     7441 atccccatcg ggcggacatt ctcgcccgcc accctcacct tcccggcgac gcggtcggtg
     7501 gcgacgtcga tcgcggtcac ggaggagtcc ctttcgccgg ggatgaaggc gcggctgccg
     7561 tcgggggcga aggcgatgct tcgcggccgc tcgccgaccg ggattcgggt gcgcacccgg
     7621 ccggtcgcgg tttcatacac cgcgaccgta tgatcctcct cggaggtcgc gtagacgagc
     7681 gaaccgtccg gggagaccgc cgtcccttcc gcctcgccgc cgaccgcggc cgccgcccga
     7741 atgcgaccgc tgcgcgaatc catgacgatg agccgtccgc tgtcctcgct ggcgacgtag
     7801 aggctgttcc cgtccgggct gaccgccacc gtctcgggat cggagatgcc gcgcagcacg
     7861 cgcagcaccc ggcccgacgc gatgtcgaac acggcaatgc cgtccgcggc cttgtcggcg
     7921 gccggcagcg tcgattcgtc gacgccgggg cctgcgaccg gagacccgct gagcgcgatg
     7981 tagaggaggc ggccatccgg gctcgcaacg atcccccggg gccgcttgcc gagcgccacc
     8041 cggccaacga cccggcgcgc ctccggatcg ataatggtca gatcgcccga catttcgttt
     8101 gtgacgtaaa cccgcacggc gggcggcgcg gtctccgccc ggcaagccgc aagtatcacc
     8161 gagagagaca gccccgcgac gcgcaagtgc atcacactcc atcctttcgc ccagtgcaga
     8221 gctgccagaa taagccgcgc tccgttcctg aggagtccga ttctccgttc gaagcgcaga
     8281 atcgattctg gcttcgatcc ggcggcagaa cgcggggccg gtctgccaac cgcgccgtcg
     8341 attgggcgga gtgtcgctgg cgcagctcgg ggcggcgcgc ccccgccgtc gacgggcgca
     8401 ccggtcctaa ttccgtttcg agattaatct ggatattcaa ttgagagttt agaattttat
     8461 ttttt
//
LOCUS       DASVQZ010000052         5564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_685588, whole genome shotgun sequence.
ACCESSION   DASVQZ010000052 DASVQZ010000000
VERSION     DASVQZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685588"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VF547_01405"
     CDS             complement(<1..1236)
                     /locus_tag="VF547_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX8261511.1"
                     /translation="MPVSPTYPGVYIQEVSSGVRTVAGVATSIAAFVGRTRRGPVNQP
                     VTCFNFGEFTRRFGGLWLDSSVSYAVDDFFANGGGQCEIVRLYEPDGNDAGVATAQFT
                     GFALEASSPGSWGDQLTATATVPDPNEPGTIAAAAKYGLGPSEIIDLTIVDAGSGAAE
                     TFRTLTAKADSERRIDRVLGDGSSLVRCPAVPANIPLQLETVPFTGGGDGLALVDDSY
                     LVSEDLKTGLYALKKADIFNLLCVPPDIRGGDLSPDVREAALRLCKSERALFIVDPPA
                     DWDDNPSDAANKAKTEQIANPLLPLADADYAALFFPNLIKRDRMRGGQLDVFPPCGAV
                     AGVMARTDVNRGVWKAPAGLAATLSNIDGLTVKLTDEENGLLNPIAINCLRTFPNIGR
                     VVWGARTMKGSDQLSDDYKY"
     gene            complement(1302..1676)
                     /locus_tag="VF547_01410"
     CDS             complement(1302..1676)
                     /locus_tag="VF547_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261512.1"
                     /translation="MADSIAWSFSAVASTGGSAKAAGKTEVDGVMIVRTTIPVDPAPA
                     TLTLQLDSAEKVSFLSISSSAYRDNLMIKSGEVELDLEGPLVLHGNAVALFSDNLTSL
                     QVTNPAGDAVELTILVGFDPTG"
     gene            complement(2073..3074)
                     /gene="trpS"
                     /locus_tag="VF547_01415"
     CDS             complement(2073..3074)
                     /gene="trpS"
                     /locus_tag="VF547_01415"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX8261513.1"
                     /translation="MAGTKTKKTVFSGVQPTGDLHIGNLIGAISLWVREQDRHDNIFC
                     VVDLHALTVPEAIEAARLRASSLDVAALYVACGIDPERSIIFLQSQVRQHAELAWILN
                     CVTPMGWMNRMTQFKSKAEKAETVGVGLYSYPILQAADILLYHTDLVPVGEDQRQHIE
                     ITQDIAQRFEGLFGYKFTRPEIMVQQVGARVMGLDDPEAKMSKSAANPRHAISILDEP
                     ATILKKLKSAKTDSRTAVDAADPGAGVRNLLTIYHAFAGGTWEDALKRFDGQGYGALK
                     IGVAEAVIAGLEPIQSRYAELRRDEAALTAMLARSAEAAAERAEATMKDVRAAVGVR"
     gene            complement(3140..3709)
                     /locus_tag="VF547_01420"
     CDS             complement(3140..3709)
                     /locus_tag="VF547_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261514.1"
                     /translation="MEERLDIETAAAAAAFAKARSRAVLLALAAREQSLRQLADSTGL
                     RLNLLHYHVARLHALGLIETVRAEPRPGRPVKIYRAVARAFFVPARLVGAEAGEKLST
                     ELRERLASARARSGGEGTLYFVGDDGRPRMRRLGGADPAEGAEFWSLLDLGRADAAAL
                     AAEMKALLGRYRGRARGRPYLVHAAFAPK"
     gene            3825..5003
                     /locus_tag="VF547_01425"
     CDS             3825..5003
                     /locus_tag="VF547_01425"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8261515.1"
                     /translation="MRTRLAAAISGLCLLIAPQAEARPAASPAPDAAAAAARCVRERA
                     RSSGFSGIVSIAQRGKPAALVALGRAAGPDSPAIAERTRFNLASASKMFTAVAVAQLV
                     DGGRVRLDDPIGRFVEGLTPEASAVTVRQLLTHSSGLGNFFAPQNLAAMIRARTASDL
                     LPLLASEKPAFPPGSRVSYSNSGFALLGVLIERVSGQSYGDYLRSRLFAPAGMADTGL
                     DPKPLATLAVGMTAARPGQGPRPGAVRTAEAVAPDSAALHPAPGATQGFGSPAGGLFS
                     TAADMHGFLEALAAHRLASPAMTAAMTAPQVEVAPATADSPARRYGFGFGVGAFEGHR
                     WFGHNGGNLGANVEVAAFPDDGWSIVVLSNRDPPAATDLFRYARGLITSPRPGDCRGA
                     "
     gene            5116..>5564
                     /gene="maiA"
                     /locus_tag="VF547_01430"
     CDS             5116..>5564
                     /gene="maiA"
                     /locus_tag="VF547_01430"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX8261516.1"
                     /translation="MSKPVLFDYFRSSASYRVRIALNLKGIDHDRVSVNLVEDGQRAP
                     AYRAVNPQGLVPALEIDGRRLTQSLAIVAYLDATRPEPPLLPADPADAAHVRALALLI
                     ACDIHPLNNLRVLRYLKDGLGREQEARDSWYRHWVAEGFAALEAMAAP"
BASE COUNT          847 a         1890 c         1949 g          878 t
ORIGIN      
        1 gtacttgtag tcgtccgaga gctggtcgga acccttcatc gtccgcgcgc cccagaccac
       61 ccgcccgatg ttggggaagg tgcgaaggca attgatcgcg atcgggttca gaaggccgtt
      121 ctcctcgtcg gtgagcttca ccgtgagccc gtcgatattg gaaagcgtcg cggcaagccc
      181 cgccggcgcc ttccagacgc cgcggttgac gtcggtgcgc gccatcaccc cggccaccgc
      241 gccgcagggt ggaaagacgt cgagctgccc gcccctcatc cggtcgcgct tgatgaggtt
      301 gggaaagaag agggcggcgt aatccgcgtc ggcgagcggc agcagcgggt tggcgatctg
      361 ctcggtcttc gccttgttgg cggcgtcaga gggattgtcg tcccaatccg ccggcggatc
      421 gacgatgaac agcgcccgtt ccgacttgca cagccggagc gcggcctcgc gcacgtccgg
      481 gctcaggtcg ccgccgcgga tgtcgggggg aacgcagagc aggttgaaga tgtcggcttt
      541 cttgagcgca taaaggccgg tcttcagatc ctcgctgacc agatagctgt cgtcgacgag
      601 cgccaagccg tcgccgccgc cggtgaacgg aacggtttcg agctgcagcg ggatattggc
      661 gggaacagcc gggcagcgga cgagcgagga accgtccccg agcacccggt cgatgcggcg
      721 ctcgctgtcc gccttggccg tcagggtcct aaaggtttcg gccgcgccgc tgccggcatc
      781 gacgatggtg aggtcgatga tctcgctggg gccgagacca tatttggcgg ccgccgcgat
      841 cgtgccgggc tcgttcggat ccggcaccgt cgccgtcgcc gtgagctgat cgccccagct
      901 gcccggactc gacgcctcga gggcgaaccc ggtgaactgc gccgtggcga cgccggcatc
      961 gttgccgtcg ggctcgtaga gtcggacgat ctcgcattgg ccgccgccgt tggcgaagaa
     1021 gtcgtcgacc gcgtaggaga ccgagctgtc gagccagagg ccgccgaaac ggcgcgtgaa
     1081 ctcgccgaaa ttgaagcagg tgacgggctg gttgaccggt ccgcgccgag tccttccgac
     1141 gaaggccgcg atcgacgtcg cgactccggc gacggtcctc acgccggagc tgacttcctg
     1201 gatgtagacg ccgggatagg tcggacttac gggcatggct tcgctccccc ttcgggcttc
     1261 gggcaaaatc caaattcaaa ccagattcgc ggcggggcgg ctcaacccgt cggatcgaat
     1321 cccaccagaa tcgtcagctc gacggcgtcg cccgccgggt tggtgacctg cagcgacgtg
     1381 agattatcgc tgaacagggc gacggcgttg ccgtgaagga ccaggggacc ctcgaggtcg
     1441 agctccacct cgccgctctt gatcatgagg ttgtcgcggt aggcggagct ggagatcgac
     1501 aggaacgaga ccttctcggc ggaatcgagc tgcagggtca gagtggcggg ggccggatcg
     1561 accgggatcg tcgtgcggac gatcatgacc ccgtcgacct ccgtctttcc cgctgccttg
     1621 gccgagccgc cggtggaggc gacggcgctg aagctccaag cgatcgaatc ggccatttag
     1681 agcccccccg tccaagtaaa gccaaggggt tacaagtaac attgagcatt gattcacgca
     1741 acgtagcccg aaagtggaac gaacttttcg gccaattcaa aatgctttcc cgaccgaata
     1801 agttgagtta tcgagacgtc gaatatattc atgtgtttac ggctgagctg gccgcaatcg
     1861 attcagtcgc tacttaaatt tgtccgaaac ggacacaata gaacaaggag tgaatcaaaa
     1921 caacttgccc tggacagctc ttagcgaagc cagtgcttgc gcgaatcgcg cttgttcatc
     1981 agaaaacgat ggaggttggc tggaatatca tcgaaagaga ggcctggcct cgggcggcgt
     2041 gcgtacttcc cgcgctgggg cgggaggcgg gattatctca cgcccaccgc cgcccgcacg
     2101 tccttcatcg tcgcctcggc gcgttcggcg gcggcctcgg ccgagcgcgc cagcatcgcc
     2161 gtcagcgcgg cctcgtcgcg gcggagctcg gcgtagcggc tctggatcgg ctccaggccg
     2221 gcgatgacgg cttcggcgac gccgatcttc aacgcgccgt aaccctggcc gtcgaaccgc
     2281 ttgagagcgt cttcccaggt gccgccggca aaggcgtggt agatggtgag gaggttcctg
     2341 accccggcgc ccggatccgc cgcgtcgacg gcggtgcggc tgtcggtctt ggccgacttg
     2401 agcttcttga ggatcgtcgc gggctcgtcg aggatcgaga tggcgtggcg cggattggcg
     2461 gcgctcttcg acatcttcgc ttccggatcg tcgaggccca tcacccgcgc gccgacctgc
     2521 tggaccatga tctcgggccg ggtgaacttg tagccgaaca gcccctcgaa gcgctgcgcg
     2581 atgtcctggg tgatctcgat atgctggcgc tggtcctcgc cgacggggac gaggtcggta
     2641 tggtagagca ggatgtcggc ggcctggagg atcggatagg aatagaggcc cacgccgacc
     2701 gtctcggcct tctccgcctt cgacttgaac tgcgtcatcc ggttcatcca gcccatcgga
     2761 gtcacgcagt tgaggatcca ggcgagctcc gcatgctggc gcacctggct ctgcaggaag
     2821 atgatcgacc gttccggatc gatgccgcag gcgacgtaga gggccgcgac gtcgaggctg
     2881 ctcgccctga gccgcgccgc ctcgatcgcc tcgggaacgg tcagcgcgtg gaggtcgacg
     2941 acgcagaaga tgttgtcgtg ccggtcctgc tcgcgcaccc acaaggagat ggcgccgatc
     3001 aggttgccga tatgcagatc gccggtcggc tggacgccgg agaacacggt cttcttcgtc
     3061 ttggtcccgg ccacgggcgc ctcttcttct ggactttcag ggcgggccta accgcgggga
     3121 gggcctcagc gcaagcggct cacttggggg cgaaggcggc gtggacgaga taggggcgcc
     3181 ccctcgcccg gccccggtag cgcccgagca gcgccttcat ctcggcggcg agcgcggcgg
     3241 cgtcggcgcg gccgagatcg agcaggctcc agaactccgc gccctccgcg ggatcggctc
     3301 cgccgagccg gcgcatgcgc gggcggccgt cgtccccgac gaaatagagc gtgccttcgc
     3361 cgccgctgcg ggcgcgggcc gaggcgagac gctcgcgaag ctcggtcgag agcttctccc
     3421 cggcctcggc gccgacgagc cgggccggga cgaagaaggc gcgcgcgacc gcccggtaga
     3481 tcttcaccgg cctgcccggc cggggctcgg cgcggacggt ctcgatcagc ccgagggcgt
     3541 ggagccgggc gacgtggtag tggagcaggt tgagccgaag cccggtcgaa tcggcgagct
     3601 ggcgaagcga ctgctcccgg gcggcgagag cgagcaggac ggcccggctg cgcgccttgg
     3661 cgaaggcggc cgcggcggcg gcggtctcga tgtccaacct ctcttccatg cgcccctcga
     3721 tcggcccgcg ggatccgtag ccacaagcta gttcaaactg gcggctttta gttgaactag
     3781 caacaagcgg accgtcgcgg gacccggcaa ggaaggaaat cgcgatgagg acaagactgg
     3841 cggccgccat ttcgggcctg tgcctgctga tcgcgcctca ggcagaggcc cggcccgcgg
     3901 cctcgcccgc gcccgacgcg gcggccgcgg cggcccgctg cgttcgcgag agagcgcgga
     3961 gctcgggctt ctccgggatc gtctcgatcg cgcaacgcgg caaaccggcc gccctcgtcg
     4021 ctctcggacg cgccgcgggc ccggacagcc cggccatcgc cgagcgcacg cggttcaacc
     4081 tggcctcggc gagcaagatg ttcaccgccg tcgccgtcgc ccagctggtc gatggcggcc
     4141 gggtgcggct cgacgatccg atcggccggt tcgtcgaggg gctcacgccc gaggcgtcgg
     4201 cggtgacggt ccgccagctg ctcacccaca gctcgggcct cggcaacttc ttcgcgccgc
     4261 agaacctcgc cgcgatgatt cgcgcccgga cggcgtcgga cctcctgccc ctcctcgcct
     4321 ccgagaagcc ggccttcccg cccggatcgc gggtcagcta ttcgaacagc ggcttcgcct
     4381 tgctcggcgt gctgatcgag cgcgtctccg ggcaaagcta tggcgactat ttgcgcagcc
     4441 gcctgttcgc gccggccggg atggccgaca ccggcctcga cccgaaaccc ctcgccacgc
     4501 tggcggtggg catgaccgcg gcccgcccgg ggcagggccc gcggccgggg gcggtccgca
     4561 ccgccgaggc ggtcgcgccg gactcggccg ccctccaccc ggcgccgggc gcgacccagg
     4621 gcttcggctc ccccgccggc gggctgttca gcaccgccgc cgacatgcac gggttcctcg
     4681 aggcgctggc cgcgcaccgc ctggccagcc cggcgatgac cgccgcgatg acggcgcctc
     4741 aggtcgaggt cgccccggcc acggccgact cgcccgcgcg ccgctacggc ttcggcttcg
     4801 gggtcggagc gttcgagggg caccgctggt tcggccacaa tggcgggaac ctcggcgcca
     4861 atgtcgaggt cgccgccttt cccgacgacg gctggtcgat cgtcgtcctg tccaaccgcg
     4921 atccgccggc ggcgaccgac ctgttccgct acgcccgcgg cctgatcacg agcccccgcc
     4981 ccggggactg ccggggggcc tgagcggcgg cgtcaggacc gcggcgcctc cggcgggcgg
     5041 ggcgccggcc cagtcgcgtg ggtccggcgg gtcatgccgc ttcctaagca cggccggccc
     5101 ggctagcgtg cggccatgag caagccggtc ctgttcgact atttccgctc gtcggccagc
     5161 tatcgcgtgc ggatcgcgct gaacctgaag gggatcgacc acgaccgggt gtcggtcaat
     5221 ctggtcgagg acgggcagcg ggcgccggcc tatcgggcgg tcaacccgca gggcctggtg
     5281 cccgccctcg agatcgacgg gcgccggctg acccagagcc tggcgatcgt cgcctatctc
     5341 gacgcgaccc ggccggagcc gccgctgctc cccgccgacc cggccgacgc cgcccatgtc
     5401 cgggcgctgg cgctgctgat cgcctgcgac atccatccgc tcaacaatct gcgggtgctc
     5461 agatatctca aggacgggct cggccgggag caggaggccc gcgacagctg gtatcgccac
     5521 tgggtggccg agggcttcgc cgccctggag gcgatggcgg cccc
//
LOCUS       DASVQZ010000053         5151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_300996, whole genome shotgun sequence.
ACCESSION   DASVQZ010000053 DASVQZ010000000
VERSION     DASVQZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300996"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VF547_01435"
     CDS             complement(<1..417)
                     /locus_tag="VF547_01435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8261517.1"
                     /translation="MRQSAPGRRKARLEVRQAGQADVAGIVALVDRAYDRLSGYSRGM
                     VRGQINNWPEGQFVAVYEGKIAGYCATMRLDERLALSPHSWDEITGDGFASRHDPTGD
                     WLYGFEMCVDPKLRGVRLGQRLYDARKALAERLELKG"
     gene            complement(414..1634)
                     /locus_tag="VF547_01440"
     CDS             complement(414..1634)
                     /locus_tag="VF547_01440"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX8261518.1"
                     /translation="MPKIKVKNPVVELDGDEMTRIIWQWIRERLIQPYLDVELLYFDL
                     GIEHRDATEDRVTVEAAEAIKAHGVGVKCATITPDEARVEEFGLKKMWKSPNGTIRNI
                     LGGVVFREPIVIENVPRLIPGWTDPIVVGRHAFGDQYKATDFRVPGPGKLTIKWEGEG
                     GETIEHEVFDFPSSGVAMGMYNLDESIRDFARACFNFGLGRGWPVYLSTKNTILKAYD
                     GRFKDIFEEIFESEFKPQFDAAGTEYQHRLIDDLVASALKWSGKFVWACKNYDGDVQS
                     DMVAQGFGSLGLMTSILMTPDGKIVEAEAAHGTVTRHYRMHQQGKATSTNPLASIFAW
                     TGGLKYRGKFDDTPEVTRFAETLERVCVETVESGQMTKDLAILIGPEQSWMTTEQFFE
                     AIRANLEKRMAATA"
     gene            1842..2579
                     /locus_tag="VF547_01445"
     CDS             1842..2579
                     /locus_tag="VF547_01445"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX8261519.1"
                     /translation="MTSLEKPDLVTTNVRYRMPNVHPEGRKFVVIAAAVTLFFWMIVE
                     WEVLGWLFAGLTLWVAAFFRDPIRTTPKGEGLIVAPADGMVTMIATVPPPPELAGEDG
                     LPPGPCVRVSIFMSVFDVHINRSPIAGEIKRVVYIAGKFLNADLDKASEDNERQHFLV
                     EGRDGVRVGFTQIAGLVARRIVPWVKAGDFVAAGQRVGLIRFGSRVDVYLPAGTSSQV
                     LLGQRTIAGETVVARAGVSEALEGVAQ"
     gene            2576..3367
                     /locus_tag="VF547_01450"
     CDS             2576..3367
                     /locus_tag="VF547_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEX8261520.1"
                     /translation="MKGPVERRGIPLRAVVPNAVTALALCSGLSGIRFAIAEKWDVAI
                     AMIMIAAVLDGMDGRIARMLNGSSRFGAELDSLSDVIAFGVSPAVILHLWSLQFWPRF
                     GWIFALAYAVCMALRLARFNARIDVEDQPHKSAGFLTGVPAPAGAALLLLPILLWLAS
                     DRQWTWLQDYRLVAPWAAAIAFLVISSLATYSWTSFRLRRRVRLEAIVIIAIVGGALL
                     TAPWETLSVLAVLYLAAIPLSILSYSRVKAQRAAASRPPEPPRGA"
     gene            4308..>5151
                     /locus_tag="VF547_01455"
     CDS             4308..>5151
                     /locus_tag="VF547_01455"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8261521.1"
                     /translation="MSWDEIIKGTSGSDIINGGSGDDYLQGRAGGDFLDGGEGNDQLQ
                     GNEGDDYIRGGAGNDFIHGGTGTDTAVYSGSVKDYAFSRNGEEFFLNHVGGSMIDGYD
                     RLISIERLVFADAVIDLTQNNAPIAFDDTASTNEDVGAYSGSSVLANDFDWEGNSMTA
                     TPGTFNGVYGTLTMNANGTYTYTPYASTQALALGQVVQDSFSYTVSDGQASSTGTLTV
                     SVAGRNDAPVANADSASTGENATVTVNVLANDSDIDNGAVLTVTAASAPAGKGTASVA
                     GNQVQ"
BASE COUNT          824 a         1726 c         1712 g          889 t
ORIGIN      
        1 gcccttgagc tcgagccgct ccgccagcgc cttgcgtgcg tcgtagagcc gctggccgag
       61 gcgcaccccg cgaagcttcg ggtcgacgca catctcgaag ccgtagagcc agtcgcctgt
      121 cggatcgtgg cgcgaggcga agccgtcgcc ggtaatctcg tcccagctgt gcggggacag
      181 cgccagccgc tcgtcgagcc gcatggtcgc gcaatagccg gcgatcttgc cttcgtagac
      241 ggccacgaac tggccttcgg gccagttgtt gatctggccg cgcaccatcc cgcgactgta
      301 gccggaaagg cggtcgtagg cgcggtcgac gagggcgacg atgcccgcga cgtcggcctg
      361 gcccgcctgc cggacctcga gccgcgcctt gcggcgcccc ggtgcgcttt gcctcacgcg
      421 gtcgccgcca tcctcttctc gagattggcg cggatcgcct cgaagaactg ctcggtggtc
      481 atccagctct gctcgggtcc aatgaggatg gcgaggtcct tggtcatctg gccggactcg
      541 accgtctcga cgcagacccg ctcgagcgtc tcggcgaagc gggtgacctc gggcgtgtcg
      601 tcgaacttgc cgcgatactt gaggccgccg gtccaggcga agatcgaggc gagcgggttg
      661 gtcgacgtcg ccttgccctg ctgatgcata cggtagtggc gggtcaccgt gccgtgcgcg
      721 gcctccgcct cgacgatctt gccgtccgga gtcatcagga tcgaggtcat caggccgagc
      781 gagccgaagc cctgagcgac catgtcggac tggacgtcgc cgtcgtaatt cttgcaggcc
      841 cagacgaact tgccgctcca cttcagcgcc gaggcgacca ggtcgtcgat caggcgatgt
      901 tggtactcgg tgccggccgc gtcgaactgc ggcttgaact cgctctcgaa aatctcctcg
      961 aaaatgtcct tgaagcggcc gtcataggcc ttgaggatgg tgttcttggt cgacagatag
     1021 accggccagc cgcgcccgag gccgaagttg aagcaggcgc gggcgaagtc gcggatcgat
     1081 tcgtcgagat tgtacatgcc catggcgacg cccgaggagg ggaagtcgaa cacctcgtgc
     1141 tcgatcgtct cgccgccttc gccctcccat ttgatggtca gcttgcccgg gccgggcacc
     1201 cggaagtcgg tcgccttgta ctggtcgccg aaggcgtggc ggccgacgac gatcgggtcg
     1261 gtccagcccg ggatcagccg aggcacgttc tcgatcacga tcggctcgcg gaagaccacc
     1321 ccgccgagga tgttgcggat ggtgccgttg ggcgacttcc acatcttctt gaggccgaat
     1381 tcctcgaccc gcgcctcgtc gggcgtgatg gtcgcgcatt tgaccccgac gccgtgcgcc
     1441 ttgatcgcct cggcggcctc gaccgtcacc cggtcctcgg tggcgtcgcg atgctcgatg
     1501 ccgagatcga agtagagcag ctcgacgtcg agatagggct ggatgagccg ctcgcggatc
     1561 cattgccaga tgatgcgggt catctcgtcg ccgtcgagct cgacgacggg gttcttcacc
     1621 ttgatcttgg gcattcggcg cacgctttcc tttggagtgc tgtcgcgcgc ggctctaggc
     1681 ggggacgggg cttcgatcaa ttcccgtcat cccggcgcag gccgggcctc gtgcggtgat
     1741 agggctctga gctttttctc gtcggggtcc ctcccgtggc cgggacgacg gccggggcgc
     1801 gacggttgtc acgtctctcg ccccggccta aaggctcgag catgacctcc ctcgaaaagc
     1861 cggacctggt caccacaaac gtccgctacc gcatgcccaa cgtgcatccg gagggccgca
     1921 agttcgtcgt catcgcggcg gcggtgaccc tgttcttctg gatgatcgtc gagtgggagg
     1981 tcctcggctg gctcttcgcc ggactgacct tgtgggtggc ggccttcttc cgcgacccga
     2041 tccggaccac gcccaagggc gaggggctga tcgtcgcgcc ggcggacggg atggtgacga
     2101 tgatcgcgac ggtgccgccg ccgcccgagc tcgccggcga ggacggcctg ccgcccgggc
     2161 cctgcgtgcg ggtctcgatc ttcatgtcgg tgttcgacgt ccacatcaac cgctcgccga
     2221 tcgccggcga gatcaagcgg gtcgtctaca tcgccggcaa gttcctcaac gcggacctcg
     2281 acaaggcgag cgaggacaat gagcggcagc atttcctggt cgaaggccgc gacggcgtgc
     2341 gcgtcggctt cacccagatc gccggcctgg tggcgcggcg gatcgtgccc tgggtcaagg
     2401 cgggcgactt cgtcgccgcc ggccagcgcg tcggcctgat ccgcttcggc agccgggtcg
     2461 acgtctatct gcccgccggg acctcgtccc aggtgctgct cgggcagcgc accatcgccg
     2521 gcgagacggt ggtcgcccgc gccggcgtgt ccgaggcgct ggagggcgtc gcccagtgaa
     2581 ggggccggtc gagcggcggg gaatcccgct gcgcgccgtc gtccccaatg cggtgacggc
     2641 tctcgccctg tgctcgggcc tgtcgggaat ccgcttcgcc atcgccgaga aatgggacgt
     2701 ggcgatcgcg atgatcatga tcgcggccgt gctcgacggc atggacggcc gcatcgcccg
     2761 aatgctgaac ggctcgagcc ggttcggtgc ggagctcgat tcgctgtccg acgtgatcgc
     2821 cttcggcgtt tcgccggcgg tcatattgca tctgtggtcg cttcaattct ggccgcgttt
     2881 cggctggatc ttcgcgctcg cctacgccgt ctgcatggcg cttcgcctcg cccggttcaa
     2941 cgcccgcatc gacgtcgagg accagccgca caaatcggcc ggcttcctca ccggcgtgcc
     3001 ggcgccggcc ggggccgcgc tgctgctgct gccgatcctg ctctggctgg cgagcgaccg
     3061 ccagtggacc tggctgcagg actacaggct ggtggcgccc tgggcggcgg cgatcgcctt
     3121 cctcgtcata tcgagcctcg ccacctattc ctggacatcg ttccggcttc gccggcgggt
     3181 ccgcctcgaa gccatcgtca tcatcgccat tgtcggaggg gcgcttctga ccgcgccctg
     3241 ggaaacgctg agcgtgctgg ccgtgctcta cctggccgcg atccccctca gcatcctgag
     3301 ctattcgcgc gtcaaagcgc agcgcgccgc cgcatcgagg ccgccggagc cgccgcgggg
     3361 ggcgtagcca cgccgagctc tccgcgcagg gcaagcgata tttcgcgcca gttgaatctt
     3421 atggtcaggt ggatcgccac cagcgcagca agcagggcca gcgtgaagaa gagcgcggca
     3481 gccaggtaag tcacagcctt atccccttac ttatccacaa ccgcacaacg cgccggtgcg
     3541 ggctttgatc cattagaccc ttgaaacacc atcatgttca cgatatgttc gaaatgtcaa
     3601 gtcctttggc gatggaccat cgcccggacg aagtcgggga tcctgggagg gaatggcgcg
     3661 gatgttcagg ctgcggaaac ggggatcggc tcgaacggcc gtcgtctctc cccctgcgcg
     3721 cccggcctcg ccggcgacgc cgtcccaaag aaaaagcccc agccctttcg ggccggggct
     3781 ctgcggtgtc ggcgggaggg gggaggggga tgccgatcac cgcggggggg tgagggcaga
     3841 acgtcgtccc cggcttcggg ttcccgaaaa aattcgagct cgaatgaaag tcgtcccgat
     3901 aaacgaagcg cctcggaatg cgtaccgagg cgcttcgccg acagctgcca tcaaaggcga
     3961 ctatctcccg tccccgattc ccctcttacc ccaaaagagc gattcggaaa agccgccaag
     4021 cgcccccgtg cgtctccgcc gggcgcccgc ggcggccgcc gcaggcgggg attccattaa
     4081 ccatatcttg gctctggaca gcggctttcg gccgcgtcaa tctggccgac ggggcagaaa
     4141 aagtcggggc gagtcgagta ctcgtcccgc tctcggttgg ggggattcaa tcgagatcgc
     4201 tgagggaggt agtgccggga catccggcgc gagcagtttc tgtccaattc atcggaaatg
     4261 cttgcgtctg accgctcgag ctatcgaaaa ccggggagga atgtaaaatg agctgggacg
     4321 agatcatcaa gggcacgagc ggcagcgaca tcatcaacgg cggaagcggc gacgactacc
     4381 tgcagggccg ggccggcggc gatttcctcg acggcggcga gggcaacgac cagctccagg
     4441 gcaacgaagg cgacgactat atccgcggcg gcgccggcaa cgatttcatc cacggcggca
     4501 ccggcaccga taccgccgtc tattcgggct cggtcaagga ttacgccttc agccgcaacg
     4561 gcgaggagtt cttcctcaac catgtcggcg gcagcatgat cgacggctat gaccggctga
     4621 tctcgatcga gcgcctggtc ttcgcggacg cggtgatcga cctcacccag aacaacgcgc
     4681 cgatcgcgtt cgacgacacc gcctcgacca acgaggacgt cggcgcctat tcgggctcga
     4741 gcgtcctcgc caacgatttc gactgggaag gcaactcgat gacggcgacg ccgggcacct
     4801 tcaacggtgt ctacggcacg ctcacgatga acgccaacgg cacctacacc tacacgccct
     4861 acgcctcgac ccaggcgctg gcgcttggcc aggtcgtgca ggacagcttc agctacaccg
     4921 tcagcgacgg gcaggcgagc agcaccggca cgctcaccgt ctcggtcgcc ggccgcaacg
     4981 acgcgccagt cgcgaatgcg gacagcgcgt cgaccggcga gaatgcgacc gtgacggtca
     5041 acgtcctcgc caacgacagc gacatcgaca acggcgccgt cctcaccgtc accgcggcct
     5101 cggcgccggc cggcaagggt accgcctccg tggccggcaa tcaggtccag t
//
LOCUS       DASVQZ010000054         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_109470, whole genome shotgun sequence.
ACCESSION   DASVQZ010000054 DASVQZ010000000
VERSION     DASVQZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109470"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF547_01460"
     CDS             <1..550
                     /locus_tag="VF547_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261522.1"
                     /translation="QLAAAPAAAAARSAPVRLASAQRPEPAVVVTARRSLRAARAEAL
                     DVEPLSFASAEAPAPLPTTLASMSMVLRHLPRTALAPRPAPHQAPAAFASSVPPPELP
                     HVEVHHRLVRVSASGGEARFFVVGHRLTPEEAAELRKAVGQAAAERQKVKFRRDRGED
                     AGSSESLFDIHIQTLVKGEEAR"
     gene            547..1104
                     /locus_tag="VF547_01465"
     CDS             547..1104
                     /locus_tag="VF547_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261523.1"
                     /translation="MTHNRILKLGAAAAVLVAGAAGAAFAHPHPDGDDDRVERVVVVR
                     DGKGGEHHGKAGKDVRRFEIIGADGGHHGKPGKHVRRFEIIDAHGGAHGKPGGPHVRH
                     FEMLGDTLARCESGEKVVDETAGDGDSKTKTVICLKGQPTAATAERLEEALARINSND
                     ELSAEQKARITTALRSAIDRARSAR"
     gene            1203..2054
                     /locus_tag="VF547_01470"
     CDS             1203..2054
                     /locus_tag="VF547_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8261524.1"
                     /translation="MSESGRVERLGPRVRRVLAPNPSPFTYTGTQTYLVGEDEVAVID
                     PGPDLPDHIDALVAAVDGRRVAAILCTHTHRDHSPASRPLQAATGAPIVGCAPLSFDD
                     AGPRADEAFDSAYRPDRVLADGDRVAADGWTLEAVATPGHTSNHLCLALPEEAALFTG
                     DHVMGWSTTVVAPPDGDMADYMASLDRLLARGDRVYYPAHGPAVEDPHAHVRRLVDHR
                     RMRERQILDHVERGEGRIPAMVADIYAEIDPRLFPAAGRSVLAHLLDLERRKLVRAEG
                     DSWTLAA"
     gene            2036..>2570
                     /locus_tag="VF547_01475"
     CDS             2036..>2570
                     /locus_tag="VF547_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX8261525.1"
                     /translation="MDLGRLNLRLWLPALAGLLLGAAIGYALRPSQRPSAPDAEEVAA
                     TVLLSVRDQGRLVPFSARFVSVASASESHLGLTARKTLIMPGTVRYGVDLARLRRSDL
                     AWDERSRTLSVTLPPLELSGPEIDLNQVQESSEGGFVMALAGSEGALDRANRQNAQDE
                     LMRQARAPAPMQLARQAA"
BASE COUNT          358 a          987 c          889 g          336 t
ORIGIN      
        1 ccagctcgca gcggcccccg ccgccgccgc cgcgcgctcg gcgccggtcc ggctcgcctc
       61 ggcccagcgg cccgagccgg ccgtggtggt caccgcccgc cgctcgcttc gcgccgcccg
      121 tgccgaggcg ctggacgtcg agccgctgag cttcgcctcg gccgaggccc cggcgccgct
      181 gccgaccacg ctcgcctcca tgtcgatggt gctgaggcac ctcccgcgca cggcgctggc
      241 gccgcgcccg gcgccgcatc aggcgcccgc cgccttcgcc tcgtcggtcc cgccgccgga
      301 gctgccgcat gtcgaggtcc atcaccgcct ggtccgggtg agcgcgagcg gaggcgaggc
      361 ccggttcttc gtcgtcggcc atcgcctgac ccccgaggag gcggccgagc ttcgcaaggc
      421 cgtcggccag gccgccgccg agcggcaaaa ggtcaagttc cgccgcgatc gcggcgagga
      481 cgcgggcagc tccgaaagcc tgttcgacat ccacatccag actctggtca agggagaaga
      541 agcaagatga cccacaatcg gatcctgaag ctcggcgccg ccgcggcggt gctggtcgca
      601 ggcgccgccg gcgcggcttt cgcccatccc catccggacg gcgacgacga cagggtcgag
      661 cgggtcgtgg tcgtccgcga cggcaagggc ggcgagcatc acggcaaggc cggcaaggac
      721 gtccgccgct tcgagatcat cggagccgac ggcggtcatc atggcaagcc cggcaagcat
      781 gtccgccgct tcgagatcat cgacgcccac ggcggcgctc acggcaagcc cggcggaccg
      841 cacgtccggc acttcgaaat gcttggcgac accctggcgc gttgcgagag cggcgagaag
      901 gtcgtcgacg agaccgccgg cgacggcgac agcaagacca agacggtgat ctgcctcaag
      961 gggcagccca ccgccgccac cgccgagcgg ctcgaggagg cgctcgcccg gatcaactcc
     1021 aacgacgagc tgagcgcgga gcagaaggcg cggatcacca ccgcgctgcg ctccgcgatc
     1081 gaccgggccc gcagcgcccg ctgacgggca agcgcgactc gggggaaggg cgggggtccc
     1141 cttacccccg ccctttctct tgcccgcttg gttttcgcgc ccggcgatgc cacatcggcg
     1201 cgatgagcga gagcggcagg gttgaaaggc tggggccgcg ggtgcgccgg gtcctcgcgc
     1261 ccaacccgtc gcccttcacc tacaccggga cccagaccta tctggtcggc gaggacgagg
     1321 tggccgtgat cgatccgggc cccgatctgc ccgaccatat cgacgctctc gtcgcggcgg
     1381 tcgacggccg gcgggtggcc gcgatcctgt gcacccacac ccaccgcgac cacagcccgg
     1441 cgagccgtcc gctccaggcc gccacgggcg cgccgatcgt tggctgcgcc ccgctcagct
     1501 tcgacgacgc cggaccccgc gccgacgaag ccttcgattc cgcctaccgg cccgaccggg
     1561 tgctcgccga tggcgaccgc gtcgccgccg acggctggac gctcgaggcg gtggcgaccc
     1621 ccggccacac gtccaaccat ctctgcctcg ccttgccgga agaggcggcg ctgttcaccg
     1681 gcgatcacgt catgggctgg tcgacgacgg tcgtcgcgcc gccggacggc gacatggccg
     1741 attatatggc aagcctcgac cggctgctcg cccgcggcga ccgcgtctat tatcccgccc
     1801 atgggcctgc ggtcgaggat ccgcacgccc acgtccgccg cctggtcgac catcgccgga
     1861 tgcgcgagcg gcagatcctc gaccacgtcg aacgcggcga gggtcggatc ccggcgatgg
     1921 tcgccgacat ctatgccgaa atcgacccgc gcctgttccc ggccgccggc cggtcggtcc
     1981 tggcccatct cctcgatctg gagcggcgca aactggtccg cgcggaggga gactcatgga
     2041 ccttggccgc ctgaacctgc gcctctggct gccggcgctc gcggggctgc tcctgggcgc
     2101 ggcgatcggc tacgcccttc gcccttcgca acggccgtcg gcgcccgacg ccgaggaggt
     2161 cgcggccacg gtcctgctct cggtgcgcga ccagggccgg ctggttccgt tcagcgcccg
     2221 cttcgtctcg gtggcgagcg ccagcgagag ccatctcgga ctcaccgcgc gcaagaccct
     2281 gatcatgccg ggaacggtcc gctacggcgt cgacctcgcc cggcttcggc gctcggacct
     2341 cgcctgggac gagcgcagcc ggaccctttc cgtcactctg ccgccgctcg agctttcggg
     2401 cccggagatc gacctcaacc aggtccagga atcgtcggaa ggcggcttcg tcatggccct
     2461 ggccggatcg gagggggcgc tcgaccgggc caaccgccag aatgcgcagg acgagctcat
     2521 gcgccaggcg cgcgcgccgg cgccgatgca gctggcgcgc caggcggcga
//
LOCUS       DASVQZ010000055         9512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_397492, whole genome shotgun sequence.
ACCESSION   DASVQZ010000055 DASVQZ010000000
VERSION     DASVQZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9512
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397492"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VF547_01480"
     CDS             complement(<1..1458)
                     /locus_tag="VF547_01480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8261526.1"
                     /translation="MTVALTRAAFEAAPARLPGPLDREHFLDAQRRHRRATWRAAAIG
                     IPAVMAAGIPLCVIVTPVLLAPFMVAAFVLEGLGLLSPGAWTWVHQAAHILPDTWAAL
                     WNGTPIAWGVLAAVFVAPGAVTMFGVWLALRLLLGRAWIPSVLSRLGARPADRRRLAE
                     RTLCNIAEELAVAAAVPPPRVFLVDSGRVNAAVVGHEVEEAVLVVSTGLLETMDRAET
                     QALVAHLIASVGNGDMRIAATFFAIFEAWGAMALMTEIPFSREARGALRRMALALRRA
                     AAGRCDPEEARAVADELLEGAGPISRGFDEYMERIEYEWRPLRNLLVDLPILVTSCIF
                     SITARLTVTLCTILLFAPPLSALWRARRKLADSAAVELTRQPAALASAVEKLHAADVE
                     LPGGHFCYFLFHDWPRIDPEKEQTEVAGHVLGMQLDPHKRLQDLRPLGSGREVTIPVS
                     GWEGWRKELKDLPILLSGLAVALPLLALMFAVSLAA"
     gene            complement(1462..2046)
                     /locus_tag="VF547_01485"
     CDS             complement(1462..2046)
                     /locus_tag="VF547_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8261527.1"
                     /translation="MTWLFLILLGLALLAAFILVAARNRLVRLRHQVVNARGQIDVQL
                     ARRHDLIPNLVELVKGAMAHEHGLLTALVEARGDAMRALQGGGGSPESAEAEDRLTSK
                     LAPLLSFVESQPHLMSLDNVRALQEELSSTENRIAFARQHYNDAATRHNEAGESFPAS
                     LFAGAADKSPLWTMPAGAERVPAVDLRFGAAAAG"
     gene            2137..3510
                     /locus_tag="VF547_01490"
     CDS             2137..3510
                     /locus_tag="VF547_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8261528.1"
                     /translation="MNKILLASAMALLAFPAFAQGLGQPERLRDAALARDETAWDIVE
                     GLTTEVGPRPAGTEAEARAREWAVRKLKSLGFANVHVEPYDMPVWVRGEERAEIVAPF
                     PQKLAVTALGRSGATPPEGIAAQVIAFDSVAELESAPDSSVRGRIVFLSHGMTATQDG
                     SQYGHFGPVRRLAPSIAARKGAAAIVIRSLGTDYHRNPHTGTQTVAEGVVPIPAGALS
                     LPDADQLKRMLKRGRPVTMRLVLAPRELGTRPSGNVVAEVRGSDPKAGIILVGGHLDS
                     WDLGTGAIDNAAGVAITAAAAKRIMEAGRPRRTIRIVWFGSEEVGGYGNKAYLAAHRD
                     EKIVFVSESDFGADRIWRMEPGFATANAGLADRIAKLLEPLGIARGTQVASGGVDLGP
                     WVTAGVAAVDLQQDGTRYFDYHHTPDDTLDKIDPEQLRQNVAAWTAMLSVVANAPEEI
                     VRKTAAP"
     gene            complement(3534..3610)
                     /locus_tag="VF547_01495"
     tRNA            complement(3534..3610)
                     /locus_tag="VF547_01495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3574..3576),aa:Pro,seq:tgg)
     gene            complement(3706..3780)
                     /locus_tag="VF547_01500"
     tRNA            complement(3706..3780)
                     /locus_tag="VF547_01500"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3746..3748),aa:Ile,seq:cat)
     gene            complement(3840..4868)
                     /locus_tag="VF547_01505"
     CDS             complement(3840..4868)
                     /locus_tag="VF547_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8261529.1"
                     /translation="MAAAGPVEGSGGPGRLDRPPEAGRYIDLPESFGRRFTIFCDTEE
                     EFDWSRPRSRDNRHCTHMRAMPDLQERFRRRGVAPVYLVDHPIATDPESVAMLCRWME
                     RGECSIGTQLHPWVNPPHDEEVTPFNSFAGNLPRALERAKIHCLTDSIEAAFGRRPTV
                     YRAGRYGVGPNTAELLEEAGYSADVSVRALFEYGDEGGPDFSRVEPRPYRVGGALVEL
                     PLSAAYVGRLRSKGRGLYRAAGRLPRGRGLLYRTGLLNRVPLTPEGVPLAEALAAVRT
                     LIGDGLRWFSLSFHSPSVEPGHTPFVRTGEDLRAFHAWWDGMLDFLEAQGIAPASIEE
                     TVAAARAA"
     gene            complement(4874..6514)
                     /locus_tag="VF547_01510"
     CDS             complement(4874..6514)
                     /locus_tag="VF547_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8261530.1"
                     /translation="MRFDDMIATVLRQSEGRPAALAAKWRQLVDLVAQGRADAGSQDD
                     AVAWLRERRAQVPQELRAEAARALGGRRVSPAALAFFAEDSASVAAPLLAAAQMEAGE
                     WLDLLPRLGPVARGLLRHRRDLPAPVVDALARFGAADFALSGEVAAGAGSGVSPAAER
                     SEAQIRDLVARIENFRRRRDAVGAAAAAAAPPVEEFQWETGPDGVILWVEGAPRGPLV
                     GQSIASIAERGQYGVDGQAAGAFEKRAPFRDARFSVPGTGAASGDWRISGVPFFDAAH
                     GNFLGYRGAARRPRLDEVAHGAPAEAPTGLFGTDLPADSLRQLIHELRTPLNAIIGFA
                     EMIDGQYMGPAALSYRARASDIMDQARRLLSAVDDLDTAARIETQRLQLAEASVDAVA
                     LLCRLHDAYERVARQRGSRIVIEIERDLPDAKVEMDAAERMFARMLAATIGLAREGET
                     ITATMAMGQDGGRKMLCLSIDRPRAIEGLDEAALLDPGYSPDGDWPGAPALGLGFALR
                     LVRNLAEAVGGALQIGAGRFSLFLPPLEPAERSSGRSG"
     gene            complement(6684..7955)
                     /locus_tag="VF547_01515"
     CDS             complement(6684..7955)
                     /locus_tag="VF547_01515"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8261531.1"
                     /translation="MKSVSRIALGVALLLGTAAVVGTAPAEAQKKKKGEAAQPAQRNF
                     NLSKEERAALLPVQTAITAKDWATAQASLPAAVAAAQGPDARYAVGRFQLEIGLGTNN
                     VQMQAQGLDNLIASGQVPAADLPVIYRNQAVLANNAGDKAKAEAAFGRVVELSPNDPE
                     ALINLAQVKNDLKKPKEAVQLLSRAIEMKRASGQTVDESWYKYALKLAFDGRRDPALR
                     QSSMALSRQLVAAYPTKENWRDALLIFRDSSSLDAAAELDVLRFMRASGALAGERDWF
                     DLADGLYRAGNYGEAKAVLDDGVAKRMIDPKKAAFAELIRLNNARLNGDRASLAGEET
                     KAMAGASGALALKIGDAFYGYGEHAKAIALYRAALAKGGVDANLVNTRIAMALLASGD
                     RAGAEAAFKSLSGARADLGAFWLAWLARSGA"
     gene            8306..>9512
                     /locus_tag="VF547_01520"
     CDS             8306..>9512
                     /locus_tag="VF547_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8261532.1"
                     /translation="MASVPPTIDSSDISPISIVEEMKTSYLDYAMSVIVARALPDVRD
                     GLKPVHRRILYACQEGGFVPGRPYRKSAKIVGDVMGNYHPHGDSAIYDALARLAQDWS
                     MRVMLIDGQGNFGSMDPDPPASMRYTEARLAKVAMTLLEDLDKDTVDFTPNYDGSRTE
                     PQVLPARFPNLLVNGAGGIAVGMATNIPPHNLGEVIAACKAYMDEPGITIEQLMEIIP
                     GPDFPTAPLILGQAGARSAYHTGRGSIIMRARHEVEEGRGERRSIVLTSIPYQLGKNT
                     LVEKIAEAAKDKRIEGVADIRDESSREGVRVVIELKRDATPEVVLNQLWRHTPAQSSF
                     PANMLAIRGGRPETLNLRDIIEAFVRFREEVITRRTKFELNKARERAHILLGLVIAVT
                     NLDEVVRIIR"
BASE COUNT         1436 a         3379 c         3403 g         1294 t
ORIGIN      
        1 ggcagcgagg ctgacggcga acatcagcgc gagcagcgga agcgcgacgg ccagccccga
       61 gagcaggatc ggaaggtcct tgagctcctt gcgccagccc tcccagccgc tcaccggaat
      121 ggtgacctcg cggccggagc cgaggggcct caggtcctgg agccgtttgt ggggatcgag
      181 ctgcatgccc aggacatggc cggcgacctc ggtctgctcc ttctcgggat cgatcctcgg
      241 ccagtcgtgg aagaggaaat agcagaagtg accgccgggc agctcgacgt cggcggcatg
      301 gagcttctcg acggccgagg cgagggccgc gggctggcgg gtcagctcga ccgcggccga
      361 atccgccagc ttgcgccgcg cccgccacag ggccgagagg ggcggcgcga acaggaggat
      421 cgtgcacaag gtgacggtga gtcgggccgt gatcgagaag atgcacgacg tgacgaggat
      481 cgggaggtcg acgaggaggt tgcgcagcgg gcgccattca tattcgatcc gctccatata
      541 ctcgtcgaag ccgcggctga tcggcccggc gccctcgagc agctcgtcgg ccaccgcccg
      601 cgcctcctcc ggatcgcagc gcccggcggc ggcccggcgc agggcgagcg ccatgcgccg
      661 aagcgcgccg cgggcctccc ggctgaacgg gatctcggtc atcagggcca tcgcgcccca
      721 ggcctcgaag atcgcgaaga aggtggctgc gatgcgcatg tcgccgttgc cgaccgaagc
      781 gatcagatgc gcgaccaggg cctgggtctc ggcccggtcc atcgtctcga gcaggcccgt
      841 gctgacgacg aggaccgcct cctccacctc gtggccgacg accgcggcgt tgacccggcc
      901 ggaatcgacc aggaagaccc gcggcggcgg gacggcggcg gcgacggcca gctcctcggc
      961 gatgttgcag agcgtccgct cggccaggcg cctccggtcg gcgggccgcg ccccgagccg
     1021 gctcaggacg ctcgggatcc aggccctgcc gagcagcagc cgaagcgcca gccagacccc
     1081 gaacatggtc accgccccgg gcgcgacgaa gacggcggcg aggactcccc aggcgatcgg
     1141 ggtgccgttc cacaaggctg cccaggtatc gggcaggatg tgcgcggcct ggtggaccca
     1201 ggtccaggcg ccgggcgaga gcaggccgag gccctcgagg acgaaggccg cgaccatgaa
     1261 cggcgcgagg agaacgggcg tgacgatcac gcagagcggg attccggcgg ccatcaccgc
     1321 cgggatgccg atcgcggccg cccgccaggt ggctcggcgg tggcgcctct gggcgtcgag
     1381 gaaatgctcg cggtcgagcg ggccgggcag ccgcgccggg gcggcctcga acgccgctcg
     1441 ggtcagcgcg accgtcatcg cctagccggc ggcggcggcg ccgaagcgga ggtcgaccgc
     1501 ggggacccgc tcggcgcccg ccggcatggt ccagagcggc gacttgtcgg cggcgccggc
     1561 gaacaggctg gccgggaagg actcgccggc ctcgttatgg cgggtggcgg cgtcgttata
     1621 atgctggcgc gcgaaggcga tgcggttctc ggtggagctc agctcctcct gcagcgcgcg
     1681 gacgttgtcg aggctcatca ggtggggctg ggattcgacg aaggagagca gcggcgcgag
     1741 cttcgaggtc agccggtcct cggcctcggc cgattcgggc gaacctccgc cgccctggag
     1801 ggcgcgcatc gcgtcgccgc gggcctcgac caaagcggtg agcaggccat gctcatgggc
     1861 catggcgccc ttcaccagct cgaccagatt ggggatgagg tcgtgccggc gggcgagctg
     1921 gacgtcgatc tggccgcgcg cgttcaccac ctgatggcgc agccggacga gccggttgcg
     1981 ggcggcgacg aggatgaagg ccgcgagcag cgccaggccg agcaggatca gaaacaacca
     2041 ggtcatgaga gcgtctcccc cggcggggat agcgaagcgg cgttaaggac cggtttcccg
     2101 ctttcccgga ctcggccttg tggctagggc gtgcccatga acaagatcct gctcgcttcc
     2161 gccatggccc tgctcgcctt tccagcgttc gcccaagggc ttggccagcc cgaacggctg
     2221 cgcgacgcgg ccctggccag ggacgagacg gcctgggaca tcgtcgaggg gctgaccacg
     2281 gaggtcggcc cgcgcccggc cggcaccgag gccgaggctc gggcgcgcga atgggcggtc
     2341 aggaagctca agtcgctggg cttcgccaac gtccatgtcg agccctacga catgccggtc
     2401 tgggtgcgcg gcgaggagcg ggcggagatc gtcgccccct tcccccagaa gctggccgtg
     2461 accgcgctcg gccggagcgg ggcgaccccg ccggagggga tcgccgccca ggtgatcgcc
     2521 ttcgactcgg tggccgagct ggagtcggcg cccgattcct cggtcagggg caggatcgtc
     2581 ttcctcagcc acgggatgac ggcgacccag gacggctcgc aatacggcca tttcgggccg
     2641 gtgcgccgcc tggcgccgtc gatcgcggcc cgcaagggcg ctgcggcgat cgtcatccgg
     2701 tcgctgggca ccgactatca ccgcaacccc cacaccggca cccagaccgt cgccgagggg
     2761 gtggtcccga tcccggccgg cgccctgtcg cttcccgacg ccgaccagct caagcggatg
     2821 ctgaagcgcg gccggccggt gacgatgcgg ctcgtcctcg ctccgcgcga gctcggcacg
     2881 cgaccgtcgg gcaacgtcgt cgccgaggtc cggggcagcg atccgaaggc cggaatcatc
     2941 ctcgtcggcg gccatctcga cagctgggac ctgggcaccg gcgcgatcga caatgccgcc
     3001 ggggtcgcca tcaccgccgc ggcggcgaaa cggattatgg aagccggccg gccgcggcgg
     3061 acgatccgga tcgtctggtt cggctccgag gaggtgggcg gctacggcaa caaggcctat
     3121 ctcgcggcgc accgcgacga gaagatcgtc ttcgtctccg aatccgattt cggcgccgac
     3181 cggatctggc ggatggagcc cggcttcgcg accgccaacg ccggcctcgc cgaccgcatc
     3241 gccaagctgc tcgagccgct cgggatcgcg cgcggcaccc aggtcgcgag cgggggcgtc
     3301 gacctcggcc cctgggtcac ggcgggcgtg gcggcggtcg acctgcagca ggacggcacc
     3361 cgctatttcg actatcacca cacgcccgac gacacgctcg acaagatcga tcccgagcag
     3421 ctcaggcaga acgtcgccgc ctggacggcg atgctctcgg tcgtggcgaa cgcgcccgag
     3481 gagatcgtcc gcaagaccgc cgcgccctga accggctgcg cagcggaagg cgatggtcgg
     3541 ggagacagga ttcgaacctg cgaccctctg ctcccaaagc agatgcgcta ccaggctgcg
     3601 ccactccccg ccttcgccct ccgggctacg gcgggcaagc ccgccaaggc tccgaaccga
     3661 ttcctcctgg cttgccagcc gaagctcccg caagggagcg aaggctggtg ggcccggagg
     3721 gattcgaacc cccaacctag ccgttatgag cggccggctc taccgttgag ctacaggccc
     3781 ggggggtcgg atagggagcg ggggcgacga tggcaagcgg ccggcgccgc ggggggggcc
     3841 taggcggccc gggccgcggc gacggtctcc tcgatcgagg cgggcgcgat tccctgcgcc
     3901 tcgaggaagt cgagcattcc gtcccaccag gcgtggaagg cgcgcaggtc ctcgccggtg
     3961 cggacgaacg gcgtgtggcc gggctcgacc gacggcgaat ggaacgacag gctgaaccag
     4021 cgcaggccgt cgccgatcag cgtccgcacc gccgccagcg cctcggcgag cggcacccct
     4081 tcgggcgtca gcgggacccg gttgagcagg ccggttcgat acagcaggcc gcggccgcgc
     4141 ggcagccggc ccgcggcccg atagaggccc ctgcccttcg agcggaggcg gccgacatag
     4201 gcggcgctga gcggaagctc gaccagcgcg ccgccgaccc ggtagggccg cggctcgacc
     4261 cgcgagaagt ccggcccgcc ctcgtcgcca tattcgaaca gcgcccggac cgagacgtcg
     4321 gccgaatagc ccgcctcctc gagcagctcg gcggtgttgg ggccgacgcc gtagcggccg
     4381 gcgcggtaga cggtcgggcg gcggccgaag gcggcctcga tcgagtcggt caggcaatgg
     4441 atcttggccc gttccaacgc cctgggaaga ttgcccgcga agctgttgaa aggcgtgact
     4501 tcctcatcgt gcggcgggtt gacccaggga tggagctggg tgccgatcga gcattcgccg
     4561 cgctccatcc agcggcacag catcgcgacc gattccggat cggtggcgat cggatggtcg
     4621 acgagataga ccggcgccac gccccggcgc cggaagcgct cctgaaggtc aggcatggcg
     4681 cgcatgtggg tgcaatggcg gttgtcgcgg cttctcggcc ggctccagtc gaactcctcc
     4741 tccgtgtcgc agaagatcgt gaagcggcgc ccgaagcttt cgggcagatc gatgtagcgg
     4801 cccgcctccg gcggccggtc gagccgccca ggtccgcccg atccctcgac ggggcctgcc
     4861 gccgccacgt ccctcagccg ctccggcccg aggagcgttc ggccggctcg agcggcggca
     4921 agaagaggga gaaccggccg gcgccgatct gcagggcgcc gcccacggcc tcggcgaggt
     4981 ttcgaaccag cctcagggcg aagccgaggc cgagggccgg agcgcccggc cagtcgccgt
     5041 cggggctgta gcccggatcg agcagggccg cttcgtcgag cccctcgatc gccctgggac
     5101 gatcgatcga caggcacagc atctttcgtc cgccgtcctg gcccatcgcc atcgtcgcgg
     5161 tgatcgtctc gccttcgcgg gcgaggccga tcgtcgcggc gagcatccgg gcgaacatcc
     5221 gctcggccgc gtccatctcg accttggcgt cgggcaggtc gcgctcgatc tcgatgacga
     5281 tgcggctgcc gcgctgccgg gcgacccgct cataggcgtc gtggagtcgg cacagcaggg
     5341 cgacggcgtc gacgctggcc tcggcgagct ggagccgctg agtctcgatc cgggcggcgg
     5401 tgtcgaggtc gtccaccgcg gacagcagcc gccgcgcctg gtccatgata tcggacgccc
     5461 gcgcccggta gctcaaggcc gccggaccca tatattggcc gtcgatcatc tcggcaaagc
     5521 cgatgatcgc gttgagcggg gtcctcagct cgtggatcag ctggcgcagg gaatcggcgg
     5581 gcagatcggt cccgaacagc cccgtcggcg cctccgccgg cgcgccgtgg gcgacctcat
     5641 cgagccgcgg ccgccgcgcg gcgccgcgat agcccaggaa attgccgtgg gcggcgtcga
     5701 agaacgggac gcccgagatc cgccagtcgc ccgacgcggc gcccgtcccg gggacgctga
     5761 agcgggcatc gcggaacggg gcgcgcttct cgaacgcgcc ggcggcctgg ccgtcgacgc
     5821 catattggcc gcgctcggcg atcgaggcga tgctctgccc gaccaggggc ccgcgcggcg
     5881 ccccttccac ccaaaggatc acgccgtcgg gcccggtctc ccactggaac tcctcgaccg
     5941 ggggcgccgc ggcggcggcg gcggcgccga ccgcgtcgcg gcggcggcgg aaattctcga
     6001 tccgggcgac gaggtcgcgg atctgcgcct cgctgcgctc ggccgccggg ctgaccccgc
     6061 tgccggcgcc cgcggcgacc tcgccgctca aggcgaaatc ggcggcgccg aaccgggcaa
     6121 gcgcgtcgac gacgggggcg ggaaggtcgc ggcggtggcg cagcagcccg cgcgcgaccg
     6181 gtccgagccg gggcagaagg tcgagccact ccccggcctc catctgggcg gcggcgagca
     6241 gcggcgccgc caccgaggcg ctgtcctcgg cgaagaaggc caaggcggcg gggctgaccc
     6301 ggcggccgcc cagcgcccgc gcggcctcgg ccctcagctc ctgcgggacc tgcgccctcc
     6361 gttcgcgaag ccaggccacg gcatcgtctt gcgagcccgc atcggcgcgg ccctgcgcga
     6421 cgagatcgac cagctggcgc catttggcgg cgagcgcggc cggccgcccc tcgctctgcc
     6481 tcagcaccgt cgcgatcatg tcgtcgaaac gcaagcgggc cagcccttcg tgaaacggac
     6541 cgcgtgccgg ccctgccaat cgttaacgcg cgctccggac gagcgaaagg gcggatttgg
     6601 gggccgtcgc aatatgggac ggcacgcgca cgcaaaaggg gcgccggatc gctcctgcgc
     6661 ccccttctcc gcctgcgccg cgtctaggcg ccgctgcgcg ccagccaggc cagccagaag
     6721 gcgccgagat cggctcgcgc cccgctgagc gacttgaacg ccgcttccgc gccggcccgg
     6781 tcgcccgagg cgagcagcgc catggcgatg cgcgtgttga cgaggttggc gtcgaccccg
     6841 cccttggcga gcgccgcccg gtagagcgcg atcgccttgg catgctcgcc atagccgtag
     6901 aaggcgtcgc cgatcttaag cgccagcgcg ccgctggcgc cggccatcgc cttggtctcc
     6961 tcgccggcga ggctggcccg gtcgccgttc agacgggcgt tgttcagccg gatgagctcg
     7021 gcgaaggcgg ccttcttcgg gtcgatcatg cgcttcgcga cgccgtcgtc gagaacggcc
     7081 ttggcctcgc cgtaattgcc ggcgcgatag aggccgtcgg cgaggtcgaa ccagtcgcgc
     7141 tcgccggcca gcgccccgct ggcgcgcatg aagcgaagga cgtcgagctc ggccgcggcg
     7201 tcgaggctgc tcgaatcgcg gaagatgagc agcgcgtcgc gccagttctc cttggtcgga
     7261 taagcggcga cgagctggcg gctgagcgcc atggacgact ggcgcagcgc cggatcccgg
     7321 cggccgtcga aggcgagctt gagggcatat ttgtaccagc tctcgtcgac cgtctggccc
     7381 gacgcgcgct tcatctcgat cgcccggctg aggagctgca cggcctcctt gggcttcttg
     7441 aggtcgttct tgacctgggc caggttgatc agcgcctcgg gatcgttcgg actcagctcg
     7501 accacccggc cgaaggcggc ttccgccttg gccttgtcgc cggcattatt ggcgaggacc
     7561 gcctggttgc ggtagatgac cggcaaatcg gcggcgggca cctggccgct ggcgatcaga
     7621 ttgtcgaggc cctgcgcctg catctgcaca ttgttggtgc cgaggccgat ctcgagctgg
     7681 aagcggccga ccgcatagcg ggcgtccggg ccctgggcgg cggcgaccgc cgcgggcagg
     7741 ctcgcctgcg cggtcgccca gtccttggcg gtgatcgcgg tctgcaccgg cagcagcgcc
     7801 gcgcgctcct ccttgctgag gttgaagttg cgctgggccg gctgcgccgc ctcgcccttc
     7861 ttcttcttct gcgcctcggc gggcgccgtt ccgaccactg ccgcggtgcc gaggagcaga
     7921 gcgacgccga gcgcgatcct ggaaacggat ttcatagaag atgcctcctg ttggacacgc
     7981 ctgagccgac ggtccgggtt cgaacggata cgaggcggcc tctaccgcca aacgaggacg
     8041 gtttctagcg ccgccgcgct gaaccgccct tgaaccgccg ctggaggaaa agccgttgca
     8101 taacagcatc ttcaccgtgg cgcctcgagc cgaaacaaaa atctcccgag ccgctggtgc
     8161 cggccgccgc gacgcctatc ttaggactct cctcgcgcgg gcgcgcatgc gtgcgcgcgg
     8221 ggtggcgccg ggcggaccct tccgctaggc tgaccttcga cccgtatctc gcgctccggc
     8281 gcttcaccac agacaggaaa tcctcttggc cagcgtcccg ccgaccatcg acagcagcga
     8341 catctccccg atctccatcg tcgaggagat gaagacctcc tatctcgact acgccatgtc
     8401 ggtgatcgtc gcgcgcgccc tgcccgacgt gcgcgacggc ctcaagccgg tccaccgccg
     8461 gatcctctac gcatgccagg aaggcggctt cgtccccggc cggccgtacc gcaagtcggc
     8521 caagatcgtc ggcgacgtca tgggcaatta ccacccgcac ggcgacagcg cgatctacga
     8581 cgccctcgcc cgcctcgccc aggactggtc gatgcgggtg atgctgatcg acggccaggg
     8641 caatttcggc tcgatggatc cggacccccc ggcgtcgatg cgttataccg aggcgcggct
     8701 cgcgaaggtc gcgatgaccc tgctcgagga tctcgacaag gacaccgtcg acttcacgcc
     8761 caattacgac ggctcgcgga cggagccgca ggtcctcccc gcccgcttcc cgaacctgct
     8821 ggtgaacggc gccggcggga tcgcggtcgg gatggccacc aacatcccgc cgcacaacct
     8881 gggcgaggtc atcgccgcct gcaaggccta tatggacgag ccgggcatca ccatcgagca
     8941 gctgatggag atcatccccg gccccgactt cccgacggcg ccgctcatcc tcgggcaggc
     9001 cggcgcccgc agcgcctatc ataccggccg cggctcgatc atcatgcgcg cccgccacga
     9061 ggtcgaggaa ggccgcggcg agcgccgctc gatcgtcctc acctccatcc cctaccagct
     9121 cggcaagaac accctcgtgg agaagatcgc ggaggcggcc aaggacaagc gcatcgaggg
     9181 cgtcgccgac atccgcgacg aatcgagccg cgaaggggtc cgtgtcgtga tcgagctgaa
     9241 acgcgacgcg acccccgagg tggtgctgaa ccagctttgg cgccacacgc ccgcccagtc
     9301 gagcttcccc gccaacatgc tggcgatccg cggcggccgg ccggaaacgc tcaacctccg
     9361 cgacatcatc gaggcgttcg tccggttccg cgaggaagtg atcacgcgcc gcaccaagtt
     9421 cgagctcaac aaggcgcgcg agcgtgccca tatcttgctc gggctcgtca ttgcggtcac
     9481 caatctcgac gaggtggttc gcatcatccg cg
//
LOCUS       DASVQZ010000056         6799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_686254, whole genome shotgun sequence.
ACCESSION   DASVQZ010000056 DASVQZ010000000
VERSION     DASVQZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686254"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF547_01525"
     CDS             <1..222
                     /locus_tag="VF547_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8261533.1"
                     /translation="ADGRVCVATLANGGITAIEPDGALEHFPFPDPICTNICFGGEDM
                     RDAWITASGTGRLYRARWPRPGLKLAFNA"
     gene            complement(245..1144)
                     /locus_tag="VF547_01530"
     CDS             complement(245..1144)
                     /locus_tag="VF547_01530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8261534.1"
                     /translation="MPELPNPVYWSIPGFIGLLLVELWVARRSQRRLHEGKDTATSLG
                     LGLGSLFAGLLSAGLVVSLSLWVYRFHLVEIGYAWWAFVLCFLLDDLAYYAFHRSAHR
                     VRWFWASHVVHHTSERYNLSTALRQTWTGFLSLNFLFRLPLFLIGFPPPMVFFCVGLN
                     LVYQYWIHTEMIRRMPAWFEAVMNTPAHHRVHHAINPRYLDRNYAGVFIIWDRLFGSF
                     EPERDDDPPCYGTVKPLGSYNLFWAAFHEWVEMARDAWRAPGLRAKLAYLVREPGWSH
                     DGSRETSDMIRARWREEKGTRTF"
     gene            1300..2754
                     /locus_tag="VF547_01535"
     CDS             1300..2754
                     /locus_tag="VF547_01535"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX8261535.1"
                     /translation="MSDLESIPGIIDQLCEMFDESVAALRGALARYLLRGERPDAEAR
                     ARGLFAYPELRIDYSFDQPPHFPPRAFGRLNQPGRYAISIARPRHFRKYLAEQLGYLV
                     RDYRVEISVGPSASEIPYPYVLDGTDDLRLDGAHAADLSRWFPATELSHIGDEVADGT
                     WDYLRHPDRPLALFDGPRVDFSLARLRHYTGTPVEHTQRYLLFTNYVRYVDEFVRWAT
                     EELRREDSPYEALSAPGGVLATRDTPDPVAEVAAGSWRKVQMPAYHLIGANGGGITLV
                     NIGVGPSNAKTICDHLAVLRPEVWLMIGHCGGLRPSQTIGDYVLAHAYLRDDHVLDGT
                     LPVEIPIPPIAEIQQALYDAAAEVTGEAPQELKRRLRTGTVVTTDDRNWELRIQSSAL
                     RFNQSRAVAIDMESATVAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANAFYERAIS
                     QHLRIGIETLRILKDQGAVLHSRKLRSFDEPPFR"
     gene            complement(2761..4503)
                     /locus_tag="VF547_01540"
     CDS             complement(2761..4503)
                     /locus_tag="VF547_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX8261536.1"
                     /translation="MRIWTGAGAAALALAGCAAPMAERRAAAPVEVKLIAFNDFHGNL
                     EPPRQSVEAPRREEGSVRVPAGGAAYLASAIDRLRSANPNHAVVSAGDMIGASPLVSA
                     VFLDEPTIHAMNLIRVDYNAVGNHEFDKGREELLRMQKGGCEKHTARQPCAVEPFPGA
                     NFKFLAANVRTADGATLFPSYGIKSFGRGRARVRIGFIGLTLKGTPTLVTPTGVAGLA
                     FEDEAETINALVPRLRSEGADAIVVLIHQGLSTRSGYNDKGCEGVDGDLLPILARLDP
                     AVDLVVSGHTHNAYVCDYGRIDRTRPILVTSAGRYGTMVTDIGLTIDPAAGRVTAKRA
                     DNLIVQSEAYGNIALTDRYPRYDPEPAVAALIRRYLDKSGPLANRVVGRVTAPATRAL
                     SPSREMVLGDIIADAHLAATRPSGAQIAFTNGFGVRADLQPAADGSVTFGQLFAIQPF
                     GNTLAVKTLTGRQIRAVLEQQFDSGSNTVTAPNMLLPSANVRYAYDLSKPAGQRILDL
                     FIDGKAVADDTVYRVAMNSFLATGGDNFTVFREGSDAAGGPVDLDALEAYIAASSPLT
                     PPVPERIRDVTPKP"
     gene            4670..5158
                     /locus_tag="VF547_01545"
     CDS             4670..5158
                     /locus_tag="VF547_01545"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX8261537.1"
                     /translation="MPAVAPYVGAMTRDPLVQHARRAALGAYAPYSRFSVGCAIESVD
                     GEVVVGANMENACYRLGVCAEIAALTAAQHAFGLAKVRRIAVAGGHVEEGALAGEAVC
                     TPCGGCRQAIYEAACLSGSDLEVISANGAGSAVEMRRISELLPSGFGPANLADAGPVR
                     PT"
     gene            complement(5145..6095)
                     /locus_tag="VF547_01550"
     CDS             complement(5145..6095)
                     /locus_tag="VF547_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEX8261538.1"
                     /translation="MATLLEPRPIASVSLTAAEHDPDGFSEELGRSFEEYGFAIIADH
                     GIPEELIRRAEEKAKAFFALPDQVKRGYHVPGGGGARGYTPFGIETAKGHKAHDLKEF
                     WHVGRELPDGHPFRGPMPDNVWPEEVASFRDTFLELYETFDRTGVKILRAIARYLGIE
                     EEYLVDSVRDGNSVLRLLHYPPIEGEPGSHIRAGAHEDINTITLLLGAEEAGLELLTR
                     EGQWIPVSPKPGELVVNIGDMLQRLTNGRLRSTSHRVVNPPRERWGLPRYSMPFFLHF
                     RSDFMIEALPGTVPPGEQPKWPPCTADEYLQERLREIKLV"
     gene            complement(6229..>6799)
                     /locus_tag="VF547_01555"
     CDS             complement(6229..>6799)
                     /locus_tag="VF547_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX8261539.1"
                     /translation="ISDQPVEVSETFEDGEKPANIIMAAAQGAQTGVKLAVAVGAMVL
                     AFVALVALANGLLGGVGGWFGQPDLSFQRIVGYVFQPIMFLIGIPWNEAGTAGGLFGT
                     KIVLNEFVAFIDLGAMQPGTLSDRSRAIVTFALCGFANFSSIAIQMAVTGGLAPNQRP
                     TIARLGIKALIAGSLANLMSAALAGLLLP"
BASE COUNT         1079 a         2283 c         2383 g         1054 t
ORIGIN      
        1 gccgacggcc gggtctgcgt cgccaccctg gccaacggcg ggatcaccgc catcgagccg
       61 gacggcgcgc tcgagcattt ccctttcccg gatccgatct gcaccaacat ctgtttcggc
      121 ggcgaggaca tgcgggacgc ctggatcacg gcctcgggca ccggccgcct ctacagggcg
      181 aggtggccgc ggccgggcct caagctggcc ttcaacgcgt gaatggggac acgtaccttt
      241 tgggtcaaaa ggtacgtgtc cccttctcct cgcgccaccg agcccttatc atgtcgctgg
      301 tctcgcgcga gccgtcgtgg ctccagccgg gctcgcggac caggtaggcg agcttggctc
      361 ggagccccgg cgcccgccag gcgtcgcggg ccatctcgac ccattcgtgg aaggccgccc
      421 agaacagatt ataggagccg agcggcttca ccgtgccgta gcacggcgga tcgtcgtcgc
      481 gctccggctc gaagcttccg aacagcctgt cccaaatgat gaacacgccg gcataattgc
      541 ggtcgagata gcgcggattg atcgcgtggt ggacccggtg gtgcgccggc gtgttcatca
      601 ccgcctcgaa ccaggcgggc atccgccgga tcatctcggt gtggatccaa tattggtaga
      661 ccaggttcag gccgacgcag aagaagacca tcggcggcgg gaagccgatc aggaagagcg
      721 ggagccggaa caggaagttg aggctgagga agccggtcca ggtctgccgc agggcggtcg
      781 agaggttgta gcgctcggac gtgtggtgga cgacgtggct cgcccagaac cagcgcaccc
      841 ggtgggccga gcggtggaag gcgtaataag cgaggtcgtc gagcaggaag cacaggacga
      901 acgcccacca ggcgtagccg atctcgacga ggtggaagcg atagacccac aggctgaggc
      961 tgaccaccag cccggcggac agcaggccgg cgaacaggct gcccagcccg aggccgagcg
     1021 aggtcgccgt gtccttgccc tcgtgcagcc gccgctgcga gcgcctggcc acccacagct
     1081 cgacgagcag caggccgatg aagccgggga tcgaccagta gacggggttg ggcagctcgg
     1141 gcacgatgtg agactagctc gcctccgccg tcattgcgag cacagcgaag caatccagcg
     1201 gctcagacgc ccgaacctgg attgcttcgt cgcttcgctc ctcgcaatga cggcctccgc
     1261 tctagccccg cgccgaaagc cgtgcgaagg gaaggggaga tgagcgatct cgaatccatc
     1321 cccggcatca tcgaccagct ttgcgagatg ttcgacgaat cggtcgcggc tctgcgcggc
     1381 gcgctcgccc gctacctgtt gcgcggcgag cggccggacg ccgaggcacg ggcgcggggg
     1441 ctgttcgcct atcccgagct caggatcgac tacagcttcg accagccgcc gcacttcccg
     1501 ccgcgcgctt tcggccgact caaccagccc ggccgctacg cgatcagcat cgcgaggccg
     1561 cgccacttcc gcaaatatct cgccgagcag ctcgggtatt tggtccggga ctatcgggtc
     1621 gagatttcgg tcgggccgtc cgccagcgag attccctatc cctacgtgct cgacggtacc
     1681 gacgacctga ggctggacgg ggcccatgcc gccgacctca gccgctggtt cccggcgacc
     1741 gagctctcgc atatcggcga cgaggtcgcg gacggcacct gggactatct caggcatccc
     1801 gaccgccccc tggccctgtt cgacggtccg cgggtcgatt tcagcctcgc ccgcctgcgc
     1861 cattataccg ggactccggt cgagcatacc cagcgctacc tgctcttcac caactacgtg
     1921 cgctacgtcg acgagttcgt ccgctgggcg accgaggagc tgcggcggga ggacagcccc
     1981 tatgaggcct tgtcggcgcc gggcggagtg ctcgcgacgc gcgatacgcc cgatccggtc
     2041 gccgaggtgg cggcggggag ctggcgcaag gtccagatgc cggcctacca tctgatcggc
     2101 gcgaatggcg gcggcatcac gctcgtcaac atcggcgtcg gcccgtccaa cgccaagacg
     2161 atctgcgacc atctcgccgt gcttcggccg gaggtgtggc tgatgatcgg ccattgcggc
     2221 gggctgaggc cgagccagac gatcggcgac tacgtcctcg cccacgccta tctgcgcgac
     2281 gaccatgtcc tcgacggcac tttgccggtc gagatcccga tcccgccgat cgccgaaatc
     2341 cagcaggcct tgtacgacgc cgccgcggag gtcacggggg aggcgccgca ggagctcaag
     2401 cggcggctga ggacgggcac ggtcgtgacc accgacgacc gcaattggga gcttcggatc
     2461 cagtcctccg cgctgcgttt caatcagtcg cgcgcggtgg cgatcgacat ggaatcggcg
     2521 acggtggcgg cgcagggcta ccgattccgg gtcccctacg gcaccttgct gtgcgtctcc
     2581 gacaagccgc tccacggcga gatcaagctg cccggccagg ccaacgcctt ctacgagcgc
     2641 gcgatcagcc agcatctgcg gatcgggatc gaaaccctgc gcatcctcaa ggaccagggc
     2701 gccgtccttc acagccgcaa gctgaggagc ttcgacgagc cgccgttccg gtagggcgcg
     2761 ctacggcttg ggagtgacgt ccctgatccg ctcgggcacc ggcggagtga gcgggctcga
     2821 ggcggcgatg taggcctcga gagcgtcgag gtcgacgggg cctccggcgg cgtcggaccc
     2881 ctcccggaac acggtgaaat tgtcgccccc ggtggcgagg aagctgttca tcgccacgcg
     2941 gtagaccgtg tcgtcggcca cggccttgcc gtcgatgaag aggtcgagga tgcgctgccc
     3001 cgccggcttc gacaggtcgt aggcgtagcg gacgttggcc gacggcagca gcatgttggg
     3061 cgcggtgacc gtgttcgagc cgctgtcgaa ctgctgctcg agcacggccc ggatctggcg
     3121 cccggtcagg gtcttgacgg cgagcgtgtt gccgaagggc tggatggcga acagctggcc
     3181 gaaggtgacg cttccgtccg ccgcgggctg gaggtcggcg cgcaccccga agccgttggt
     3241 gaaggcgatc tgcgctcccg aagggcgggt ggcggcgaga tgggcgtcgg cgatgatgtc
     3301 gcccagcacc atctcccgcg acgggctcag cgcgcgcgtc gcaggcgcgg tgacccggcc
     3361 gacgacgcgg ttcgcgagcg gccccgactt gtcgagatag cgccggatga gcgccgccac
     3421 cgcgggctcc gggtcatagc gcggataccg gtccgtgagc gcgatattgc cataggcctc
     3481 gctctgcacg atcagattgt ccgcccgctt cgcggtcacc ctgccggcgg ctggatcgat
     3541 ggtgaggccg atgtcggtga ccatggtgcc gtagcggccc gcgctggtga cgaggatggg
     3601 ccgcgtccgg tcgatccggc catagtcgca gacataggca ttgtgggtat ggccggagac
     3661 gaccagatcg acggccgggt cgagccgggc gaggatcggg agcaggtcgc cgtcgacgcc
     3721 ttcgcagccc ttgtcgttgt agcccgaccg ggtcgacagc ccctggtgga tgagcaccac
     3781 gatcgcgtcg gcgccctcgg accggagcct gggcaccagc gcgttgatcg tctccgcctc
     3841 gtcctcgaag gccaggccgg cgacgccggt gggagtgacg agcgtcggag tccccttaag
     3901 cgtcagcccg atgaagccga ttcggacgcg ggcacgaccg cgcccgaagc tcttgatgcc
     3961 ataagagggg aagagcgtcg cgccgtcggc ggtgcggacg ttggccgcga ggaacttgaa
     4021 gttcgcgccc ggaaacggct cgaccgcgca gggctgccga gcggtatgct tctcgcagcc
     4081 gcccttctgc atccggagca gctcctcccg ccccttgtcg aattcgtgat tgccgaccgc
     4141 attatagtcg acccggatca ggttcatcgc atggatggtc ggctcgtcga gaaagacggc
     4201 cgagacgagc ggcgacgcgc cgatcatgtc gccggccgag acgacggcat ggttgggatt
     4261 ggcggagcgc agccggtcga tggcggaggc caggtaggcc gcgccgcccg cgggcacccg
     4321 caccgagcct tcctcccggc gcggcgcttc gacggattgc ctgggcggct ccagattgcc
     4381 gtggaagtcg ttgaaggcga tcagcttaac ctcgaccggg gcggccgcgc gccgctccgc
     4441 catcggcgcc gcgcagcccg ccagggccaa cgctgccgca cccgcacccg tccagatccg
     4501 catggaaagc ttctccctct cggcgcgctg gtggcacgct cctcgagcgc tgtcactagg
     4561 ggacgacatg ggcgcgtcgc cccagcgaag gccggggccg tagcaggcga ggggcgccgc
     4621 tgcacttcac tccaggggat ccccgcttcc ccgggacgag acgctaggca tgccggccgt
     4681 tgccccctat gtcggcgcga tgacccgaga tcccctcgtc cagcacgccc gccgggcggc
     4741 gctcggcgcc tatgcccctt attcgcgctt cagcgtcggc tgcgcgatcg aatcggtcga
     4801 cggcgaggtc gtggtcggcg ccaatatgga gaacgcctgc taccggctcg gcgtctgcgc
     4861 cgagatcgcc gccctgaccg ccgcccagca cgccttcggc ctggcaaagg tgaggcggat
     4921 cgccgtcgcc ggggggcatg tcgaggaggg cgcgctcgcg ggcgaggcgg tctgcacccc
     4981 ctgcggcggc tgccgccagg cgatctacga ggcggcctgc ctgtcgggct ccgacctcga
     5041 ggtgatctcc gccaacgggg cgggcagcgc ggtcgagatg cggcgcatct ccgagctgct
     5101 cccgagcggc ttcgggccgg ccaatttggc cgacgccggg ccggtcagac caacttgatc
     5161 tccctcagcc gctcctggag atattcgtcg gcggtgcagg gcggccactt cggctgctcg
     5221 cccggcggca ccgtcccggg cagggcctcg atcatgaagt ccgagcggaa gtgcaggaag
     5281 aagggcatcg agtagcgagg aagcccccag cgctcgcgcg gcgggttgac gacccggtgg
     5341 gaggtcgagc gcagccgccc gttggtgagg cgctggagca tgtcgccgat gttgacgacg
     5401 agctcgcccg gcttcggcga gaccgggatc cactggcctt ccctggtgag cagctcgagc
     5461 ccggcctcct cggcgccgag cagcagtgtg atggtgttga tgtcctcgtg cgctccggcg
     5521 cggatgtggc tgccgggctc gccctcgatc ggtggatagt ggagcaatct gaggaccgaa
     5581 ttgccgtcgc gcaccgaatc gaccagatac tcctcctcga tgccgaggta gcgggcgatc
     5641 gccctcaata tcttgacccc ggtccggtcg aacgtctcgt aaagctcgag gaaggtgtcg
     5701 cggaagctcg ccacctcctc cggccagaca ttgtccggca tcgggccgcg gaaggggtgg
     5761 ccgtcgggca gctcccgacc gacatgccag aattccttga ggtcgtgggc cttgtggccc
     5821 ttggcggtct cgatcccgaa cggcgtgtag ccgcgcgcgc cgccgccgcc ggggacgtgg
     5881 tagccgcgct tcacttggtc gggcagggcg aagaaggcct tcgccttctc ctcggcgcgg
     5941 cggatgagct cctcggggat gccgtggtcg gcgatgatcg cgaagccata ttcctcgaag
     6001 cttcgaccga gctcctcgga aaagccgtcg ggatcgtgtt cggccgccgt cagcgagacg
     6061 gaggcgatgg ggcggggttc taggagggtg gccatgggcg tcttctaacc ccaacgacgc
     6121 aactcgtcac cccagccgtc atcccagcgt cggctgggat gtcggcgagc gaaaggcgct
     6181 ggcggtcttc accccccggg ggccccagcc tgcgctgggg cgacgagttc acggcagcag
     6241 cagccccgcc agcgccgcgc tcatcagatt ggcgagcgag ccggcgatca gcgccttgat
     6301 gccgagccgg gcgatcgtcg gccgctggtt cggcgcgagg ccgccggtca ccgccatctg
     6361 aatcgcgatc gagctgaaat tggcgaagcc gcatagcgcg aaggtgacga tcgcgcggct
     6421 gcggtcgctc aacgtgcccg gctgcatcgc gccgagatcg atgaaggcga cgaactcgtt
     6481 gagcacgatt ttggtgccga acagcccgcc ggccgtccca gcctcattcc aggggatccc
     6541 gatcaggaac atgatcggct ggaagacgta gccgacgatg cgctggaagc tgagatcggg
     6601 ctggccgaac cagccgccga ctccgccgag caggccgttg gcgagcgcca ccaaagcgac
     6661 aaaggcgagc accatggcgc cgaccgcgac ggcgagcttc acaccggtct gggcgccctg
     6721 ggccgcggcc atgatgatgt tggccggctt ttcgccgtct tcaaaggttt cggagacttc
     6781 caccggttgg tcggagatc
//
LOCUS       DASVQZ010000057         5796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_686753, whole genome shotgun sequence.
ACCESSION   DASVQZ010000057 DASVQZ010000000
VERSION     DASVQZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686753"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..577)
                     /locus_tag="VF547_01560"
     CDS             complement(140..577)
                     /locus_tag="VF547_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8261540.1"
                     /translation="MPRMIFVNLPVAELDRSIAFYKAIGAEQNRQFSDETAAMMVFSD
                     AIHVMLLTHGKFRQFTAKRIADSHETVQVLLALSCESREAVDEITEKAIAAGGRETRD
                     RDDHGWMYGRAFEDPDGHTWEPTWMDLDAAPQAVAQPDAKAEA"
     gene            642..1340
                     /locus_tag="VF547_01565"
     CDS             642..1340
                     /locus_tag="VF547_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX8261541.1"
                     /translation="MKLRKITEAGPAGPKRRYDVACGTAHALDLVGERWALLVMRELM
                     LGPKRFSDLRSDLPGISANVLTQRLEGLEAAGLLVRRRLPPPASTQVYELTPWGYEAE
                     PIVQSLGKWAARSPAHDPTLPISATSIMLSFRTMFDPQRAGNFRARIGFRLGAESFLA
                     KIEDGRFENGRGDFDGADVVFTGTPTGLAAFVYGGQPIEVLEDVGLLKLEGDPEVAAR
                     FARLFPLPPKVGAI"
     gene            complement(1471..1546)
                     /locus_tag="VF547_01570"
     tRNA            complement(1471..1546)
                     /locus_tag="VF547_01570"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1511..1513),aa:Ala,seq:cgc)
     gene            1775..2854
                     /locus_tag="VF547_01575"
     CDS             1775..2854
                     /locus_tag="VF547_01575"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX8261542.1"
                     /translation="MGYGSDDRYDNRWRDRDEHGGWRGEGRGGYGRGDDRGFFDRAGD
                     EVRSWFGDDEAQRRREMDERRWEQEQRMSGGRSNDPRSGGSSWMGSGWGNQPGESWHR
                     DRPGVRGGWDRDRSSEREGWFSSSAGGERGERWGGGSDWRDEGFGNDRVTHGSSGFGG
                     EPHHGRRFDRNDSGRVGTSSPMGQSFAGYGGRDELTRGGFGGDIHDPHYSEWRRRQID
                     ELDRDYDEYRREHQSKFEAEFGGWRQKRQSQRQMLGKVTEHMEVVGSDGSHVGKVDKV
                     RGDRIILAKSDPNAGGMHHSVPCGWVESVEDKVILNRTAESAMDSWRNEDTGRALFER
                     PDQGSEGPHALDRSFSGTYGGRNDD"
     gene            3000..3998
                     /locus_tag="VF547_01580"
     CDS             3000..3998
                     /locus_tag="VF547_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8261543.1"
                     /translation="MSTETTRAWHLKSRPQGMPTMDNFELKEAPLGELRDGWIRVENK
                     WLSVDPYMRGRMNDVKSYVPSFEVGAPMDGGAVGTVVESRSPDFAVGETVFHMMGWRE
                     AAAGPAERFNKLPPLPVEDHQWLGNLGLTGGTAYFGLLRAAQAKEGDTVFVSAAAGGV
                     GSAVVQIAKAKGMTVIGSAGGAEKCAWVRELGADEVIDYKAEPIVPALIRAAPKGIDV
                     YFDNVGGEHLDSAFAAARQNARFAICGMIEGYNGAPPYAFKYIMRVIAARIMLKGFIY
                     TDYLAEMADFYRDMGGWIAAGKVTSRETVRDGIEAMPEAFLDLFRGANIGKMLVRL"
     gene            complement(4136..5515)
                     /locus_tag="VF547_01585"
     CDS             complement(4136..5515)
                     /locus_tag="VF547_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500068.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8261544.1"
                     /translation="MQSVQLLGEDDFDGWRDAARTLAGSGVPASEVSWSTGGGGDLFA
                     APTELPRPGAPFSVPRAFLTLAETAICHSDPERFALLYALLLRLRERPGLLSDSADPL
                     VQRLERMAKAVRRDVHKMRAFLRFREVETGEGPRFVAWFEPEHHIVRHNAGFFVRRFA
                     SMRWSILTPEVSLHWDGETLIEGPGARRADAPDGDPLEAVWKTYYASIFNPARLKTGA
                     MLKEMPKKYWKNMPETALVGQLVAGAQAREAKMVEAARGQIGGNLSAAWEAVREEAKG
                     CTRCHLYKCASQTVFGEGPLDARILFVGEQPGDQEDLAGRPFVGPAGQLFDRALGEAG
                     VDRSAAYVTNAVKHFKFEQRGKRRIHSKPDAGEIEACRWWIEQERLLIRPPLTVALGA
                     TAARSIFGKAVTITAMRGRAHALPEGGEAWVTVHPSFLLRVQDNKEAEYARFVADLRL
                     FGERAKALA"
     gene            complement(5590..>5796)
                     /locus_tag="VF547_01590"
     CDS             complement(5590..>5796)
                     /locus_tag="VF547_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HEX8261545.1"
                     /translation="LGVKAVDRLLESRRWRRLRLEDVARLTVSIAKVRPFICADGWRP
                     TVLTDRADLAAWLKPKENQLELFA"
BASE COUNT          932 a         1877 c         2092 g          895 t
ORIGIN      
        1 ggcggcgaca tcgggatccg gcgtcagcag ctcgtaccag atgaagctgc cgtggcggtt
       61 tcggctctca gacattcttg tcccctataa tccaggttaa tcgcggcggc gattcgtccc
      121 gccgcggact ggccacgcct caggcctcgg ccttcgcgtc gggctgggcc acggcctggg
      181 gcgccgcgtc gaggtccatc caggtcggct cccaggtatg gccgtcggga tcctcgaagg
      241 cgcggccgta catccagcca tgatcgtccc tgtcgcgagt ctcgcggccg cccgcggcga
      301 tcgccttctc ggtgatctcg tcgacggcct cgcggctttc gcaggacagg gcgagcagca
      361 cctgcaccgt ctcgtgcgag tccgcgatcc gcttggcggt gaactgccgg aacttcccgt
      421 gggtgagcag catcacatgg atggcgtccg agaagaccat catcgccgcg gtttcgtcgc
      481 tgaactggcg attctgctcg gcgccgatcg ctttgtagaa ggcgatggag cggtcgagct
      541 cggccacggg caggttcacg aagatcatcc tgggcatgtc gtccctcctg gtgcgaatcg
      601 atgtttgacg ctgatgggcg attcagttat aaaaagcaac tatgaagtta agaaaaataa
      661 ctgaagcggg cccggccggc cccaagcggc gctacgacgt cgcctgcggg accgcccacg
      721 cgctcgatct ggtcggcgag cgctgggcgc tgctggtgat gcgcgagctg atgctgggcc
      781 ccaagcggtt cagcgacctc cgctcggacc tgcccggcat cagcgccaac gtcctcaccc
      841 agcggctgga ggggctggag gcggcggggc tgctcgtccg ccggaggctc ccgccgcccg
      901 cgtcgaccca ggtctacgag ctgaccccct ggggctatga ggccgagccg atcgtccagt
      961 cgctcggcaa atgggcggcg cgctcccccg cgcacgaccc aaccctgccg atcagcgcca
     1021 cgtcgatcat gctgtcgttc cggacgatgt tcgacccgca gcgggcgggg aatttccggg
     1081 cccgaatcgg gttccggctg ggggccgaga gcttcctggc gaagatcgag gatgggcggt
     1141 tcgagaatgg aaggggcgac ttcgatggag cggatgtcgt gttcaccggg actccgacgg
     1201 gtctggcggc tttcgtttac ggcggccagc cgatcgaagt cctggaagac gtaggattgc
     1261 tgaagctgga gggggatccg gaggtcgcgg caaggttcgc gaggctcttc cccctgccgc
     1321 cgaaggtggg cgcgatttag cgcagccgct ttagcgcgaa cgcgcggctt tccgatgagg
     1381 cagtagccct ccttcgctaa agctacggcg ggcagccttc cccctccgac ctccggctgg
     1441 cttgccagcc gaagcccgaa gggcgaaggc tggtggagct gaggggaatc gaacccctga
     1501 cctctgcagt gcgattgcag cgctctccca tctgagctac agccccgcgc cttttcggcg
     1561 gccaggtttc cccgtggata gcggggctgg gaggcgccca gatagcgatc ccgccgcgct
     1621 tgcgcaatgc cgggattcgg ttgggcgggg agtgcccacg ggtcggctct taacatggaa
     1681 cagtctcccc gaagccccgt tccccttgca tcccgcggcg cgcggatggg ccgcgccggt
     1741 atccccgcgg gcacctgatc acgaggagag tatcatgggt tacggcagcg acgatcgcta
     1801 tgacaatcga tggcgcgacc gcgacgagca tggcggctgg cgcggcgaag gacgcggcgg
     1861 ctacggccgc ggcgacgacc gcggcttctt cgaccgggcc ggcgacgagg tccgctcctg
     1921 gttcggcgac gacgaggccc agcgccggcg cgagatggac gagcgccgct gggagcagga
     1981 gcaacggatg agtggcggcc gaagcaacga tccgcgctcc ggcggatcga gctggatggg
     2041 cagcggctgg ggcaaccagc ccggcgagag ctggcaccgc gaccgccccg gcgtacgcgg
     2101 cggctgggac cgcgaccgtt cgagcgagcg cgaaggctgg ttctcgagct cggccggcgg
     2161 cgagcgcggc gagcgctggg gcggcggctc cgactggcgc gacgagggct tcggcaacga
     2221 tcgcgtcacc cacggcagct ccggcttcgg cggggagccg caccacgggc gccgcttcga
     2281 ccgcaacgac agcggccggg tcgggacctc gagtccgatg ggccagagct tcgccggcta
     2341 cggcggccgc gacgagctga cccgcggcgg cttcggcggc gacatccacg acccgcatta
     2401 ttccgaatgg cggcggcggc agatcgacga gctcgaccgc gactatgacg aatatcgccg
     2461 cgagcatcag tcgaagttcg aggccgagtt cggcggctgg aggcagaagc gccagagcca
     2521 gcgccagatg ctcggcaagg tgaccgagca tatggaggtg gtcggctccg acggcagcca
     2581 tgtcggcaag gtcgacaagg tccgcggcga ccgcatcatc ctcgccaagt cggatcctaa
     2641 cgcgggcgga atgcaccaca gcgttccgtg cggctgggtc gagagcgtcg aggacaaggt
     2701 gatcctgaac cgcaccgccg agagcgcgat ggactcgtgg cgcaacgagg acacgggacg
     2761 cgccctgttc gagcgtccgg accagggaag cgagggcccg cacgccctcg accgaagctt
     2821 ctccggaacc tacggcggcc gcaacgacga ctgagcggcg tcggccggtg atgaggcccg
     2881 gcttccccag ggaggccggg ccttttttcg tcattgcgag gagcgaagcg acgcggcaat
     2941 ccacgggcga ggcgcggcac tggattgctt cgcttcgctc gcaatgacgg gagagagcga
     3001 tgagcaccga gaccacccgc gcctggcatt tgaagtcgcg cccccagggc atgcctacga
     3061 tggacaattt cgagctgaag gaggcgccgc tcggcgagct ccgggacggc tggatccggg
     3121 tcgagaacaa gtggctgtcg gtcgatccct acatgcgcgg gcggatgaac gacgtgaaga
     3181 gctacgtgcc ctcgttcgag gtcggagcgc cgatggacgg cggcgccgtc ggcaccgtgg
     3241 tcgaaagccg gtcgcccgac ttcgcggtgg gcgagaccgt cttccacatg atgggctggc
     3301 gcgaggccgc ggcggggccg gcggagcggt tcaacaagct gccgccgctg ccggtcgagg
     3361 accatcagtg gctgggcaat ctcggcctca ccggcggcac cgcctatttc gggctgctga
     3421 gggcggcgca ggcgaaggag ggggacacgg tcttcgtctc cgccgcggcg ggcggggtcg
     3481 gatcggcggt ggtccagatc gccaaggcaa agggcatgac cgtgatcgga tcggcgggcg
     3541 gggccgagaa atgcgcctgg gtcagggaac tcggcgcgga cgaggtgatc gactacaagg
     3601 ccgagccgat cgtcccggcg ctgatcaggg cggcgccgaa ggggatcgac gtctatttcg
     3661 acaatgtcgg cggcgagcat ctcgactcgg cgttcgcggc ggcgcgtcag aacgcgcgct
     3721 tcgccatctg cggaatgatc gaaggctata acggcgcccc gccctacgcc ttcaagtata
     3781 tcatgcgcgt catcgccgcc cggatcatgc tgaagggctt catctacacc gactatctgg
     3841 cggagatggc cgacttctac cgcgacatgg gcggctggat cgcggccggc aaggtcacca
     3901 gccgggagac ggtgcgcgac gggatcgagg cgatgcccga ggccttcctc gacctgttcc
     3961 gcggcgccaa catcggcaag atgctggtga ggctctgatt ggctgcgagg ggtccttcga
     4021 tacgggcctt cgccttcgct cagtccctac tcaggatgag cggttgcgga catgtccttt
     4081 ccgctcatcc tgagtagccg gtgagcctgg cgaaccggcg tatcgaagca caacgtcagg
     4141 ccagcgcctt cgcgcgttcg ccgaacaggc gcaggtccgc gacgaagcgg gcatattcgg
     4201 cctccttatt gtcctggacc ctgagcagga agctggggtg gacggtaacc caggcctcac
     4261 ccccttcggg cagggcgtgg gcgcggccgc gcatggcggt gatcgtcacc gccttgccga
     4321 agatcgagcg ggcggcggtg gcgccgagag cgacggtgag cgggggccgg atcagaagcc
     4381 gctcctgctc gatccaccag cggcaggcct cgatctcgcc ggcgtcgggc ttggagtgga
     4441 tccggcgctt gccgcgctgc tcgaacttga aatgcttgac cgcattggtg acgtaggcgg
     4501 ccgaccggtc gacccccgcc tcgccgaggg cgcggtcgaa cagctggccg gcagggccga
     4561 cgaacggccg gccggcgagg tcctcctgat cgccgggctg ctcgccgacg aacaggatcc
     4621 gcgcgtcgag cgggccttcg ccgaacaccg tctgggaggc gcatttgtac aggtggcagc
     4681 gggtgcagcc cttcgcctcc tcgcggaccg cctcccaggc ggcgctgaga ttgccgccta
     4741 tctgtcctcg tgccgcctcg accatcttcg cctcgcgcgc ctgcgccccc gccaccaact
     4801 gccccaccaa cgccgtctcg ggcatgtttt tccagtattt cttcggcatc tccttgagca
     4861 tcgcgccggt cttgaggcgg gccgggttga agatggaggc ataataggtc ttccagaccg
     4921 cctcgagcgg atcgccgtcc ggcgcgtcgg ctcggcgcgc gccgggcccc tcgatcaggg
     4981 tctcgccgtc ccagtggagc gagacctcgg gggtcaggat cgaccagcgc atgctggcga
     5041 agcggcggac gaagaagccg gcattgtggc ggacgatgtg atgctcgggc tcgaaccagg
     5101 cgacgaagcg ggggccttcg ccggtctcga cctcgcggaa gcgcaggaag gcgcgcatct
     5161 tgtggacgtc gcgccggacc gccttggcca tgcgctcgag ccgctggacg agcgggtcgg
     5221 cggagtcgga cagcaggccc ggccgctcgc gaagccgaag gaggagcgcg tagagcaggg
     5281 cgaagcgctc cgggtccgag tggcagatgg cggtttcggc gagggtgagg aaggcgcgcg
     5341 ggacggagaa gggggcgcct gggcgaggca gctcggtggg ggcggcaaac aggtcgccgc
     5401 cgccgcccgt gctccagctc acctcggagg cgggcacgcc cgatccggca agggtgcggg
     5461 cggcgtcacg ccagccgtcg aaatcgtctt cgccgaggag ttggaccgac tgcatctcgt
     5521 catgccagcg ccggctggca tccattccga ttcagcatgg accccagctt tcgctggggg
     5581 gacgaaggat catgcgaaga gttccagctg attctccttg ggtttgagcc aggccgcgag
     5641 atccgctcta tccgtcaaca cggtcggccg ccagccgtcc gcgcagatga acggacggac
     5701 cttggcgatc gagacggtca gccgggcgac gtcctccaga cgcagccttc gccagcggcg
     5761 gctctcgagg agcctgtcga ccgccttgac gccgag
//
LOCUS       DASVQZ010000058         7851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_493241, whole genome shotgun sequence.
ACCESSION   DASVQZ010000058 DASVQZ010000000
VERSION     DASVQZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493241"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VF547_01595"
     CDS             complement(<1..766)
                     /locus_tag="VF547_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8261546.1"
                     /translation="MSADAAPTRFTLYRDAGSRFGGPLRRAWGRKWVRRLAYLAAIPI
                     LLYFLLWLLFARNLPSAETLLTYQPPLPTNVRGIDGEPVQSFARERRVELSYDEFPQQ
                     LIDAFTSAEDKTFFSHGGIDYPGLVGAVGDYISKVGSGERARGGSTITQQVAKNLLLG
                     DEYSVTRKIKEAFLARRIENVLTKQQILALYLNQIFLGRNAYGVQAASRAYFDKDVGQ
                     LQLHEAAYLAILPKAPSNYDPQRRTQRALDRRNWVLA"
     gene            853..1140
                     /locus_tag="VF547_01600"
     CDS             853..1140
                     /locus_tag="VF547_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX8261547.1"
                     /translation="MEIEFDPEKRDQTIKRRGLDFAEAERIFNVYALTLEDRRFDYGE
                     DRFVTYGMLNGELVACVWTSRAEGRTRRIISLRKARKNEREIYYLNRPEPR"
     gene            1097..1420
                     /locus_tag="VF547_01605"
     CDS             1097..1420
                     /locus_tag="VF547_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX8261548.1"
                     /translation="MSEKSTTSTGRNPDEHADDWDPDTAPILTHESAARAQISIGDRI
                     VREADPPLGTRRGRPPKPSGERKVLVSLRLAPDIVEWFRATGPGWQSRIEELLRREVR
                     TAAES"
     gene            complement(1417..2148)
                     /locus_tag="VF547_01610"
                     /pseudo
     CDS             complement(1417..2148)
                     /locus_tag="VF547_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(2270..2806)
                     /locus_tag="VF547_01615"
     CDS             complement(2270..2806)
                     /locus_tag="VF547_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8261549.1"
                     /translation="MEFDPQPRLAGDLIEFRPLVPADWDALYAVASDPAIWEQHPAPD
                     RWREPAFRGFFEDALASGGALVAIDRADGSVIGSSRYHGFDPERSEVEIGWTFLARSR
                     WGGVFNGEMKRLMLDHAFRFVESVLFLVGPQNRRSRRAVEKIGGKLDGRAERFGRESL
                     VYRLRSADWADRAAGTAA"
     gene            complement(2850..4007)
                     /locus_tag="VF547_01620"
     CDS             complement(2850..4007)
                     /locus_tag="VF547_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261550.1"
                     /translation="MRPSLFALLAAAFALPAAIPAACAAPGAETEAGQGEVTWTIEAD
                     PRQRAKGEIQFALTRRTANSRWMHSHTRPLAGLDGLSAEQLASPTGAPVSFRLNRDAG
                     AIACEGVARRWHGTGECRFQPSAAFAASLAERGYGRATDAQLFSLALHDLGHAYLEEL
                     KRQGYARPTVADIIRAGDHGAGLDYLKGMGGHGYRVGTLDSLIMMRDHGVTPDYVAEL
                     ARFGLRDLSSEQVGRLRDHGITPDFVAGFRTAGYDVPLDQLIRLRDHGVTPDYVRQLA
                     GYDLKSVPADQLVQLRDHGVMPDFVGEAARHGYRFSTAELIRLRDHGVTPDYLGELKA
                     LGYGGLASDDIVKLRTHGVSADFIRRVNAAGGGRRSAAELVSLRIGGWAER"
     gene            complement(4176..5150)
                     /locus_tag="VF547_01625"
     CDS             complement(4176..5150)
                     /locus_tag="VF547_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8261551.1"
                     /translation="MARVALLSNPRSTGNRALLPRVRSFCAAHKDIFHYEVEHVDQIG
                     TALRTIAMVEPRVLVINGGDGTVQAALTEIHNGRHFGDSPPPVAVLPNGKTNLIALDL
                     GADGDPLETLERVLAIARSDLAPHIVQRELITLSGGSGHGKPVLGMFLGGAYLADTML
                     YCRNKLYPLGLPNGVAHVLALLLGLLGVVLGRRASFLPPPPATLRVSGMKSGELNGRF
                     AFLMVTTLQRLLFKGHMPGAGARTGAMQLLVIDRNIGSLLGAFFAAVRGKLGRKRLSG
                     VHLERGDEIRIEGAGSNVLLDGELFQAECGRPIVLKPSRPVPFVKLAA"
     gene            complement(5154..5663)
                     /locus_tag="VF547_01630"
     CDS             complement(5154..5663)
                     /locus_tag="VF547_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX8261552.1"
                     /translation="MSRLFLSAAAVAAALTLPAAPAAAALGPDAAACSAGANRPAMLV
                     SVNGFKNRVGKLRIQLYGGNPSQFLERGAKLKRIDLPVTRAGAMNVCVAVPSTGTYAI
                     AVRHDADNNGKTNWNDGGGFSNNPKLSLLKLKPSHRAAAVAVGRGVKVVPVVLNYRSG
                     LTIAPIGQK"
     gene            5983..6234
                     /locus_tag="VF547_01635"
     CDS             5983..6234
                     /locus_tag="VF547_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261553.1"
                     /translation="MKMIVKGAMVAGLMALAACGENTPAENAADNVEAVSENVADNLE
                     EAADNMSNDTAADATENKADAVREAGENKADAIEANSAD"
     gene            6516..6872
                     /locus_tag="VF547_01640"
     CDS             6516..6872
                     /locus_tag="VF547_01640"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX8261554.1"
                     /translation="MKNRRFAARLGFALAGLLLVFRREKSFRAQAGLAAAAVVALLVL
                     RPGPLWAALVASAATLVLALELVNSALEYLLDRLHPAHCPEIGAAKDAAAAAVLVASL
                     AALGVGALMLFAVLSG"
     gene            6954..7202
                     /locus_tag="VF547_01645"
     CDS             6954..7202
                     /locus_tag="VF547_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8261555.1"
                     /translation="MNLIIILIVGAVVGWLAGIITRSGGGLLFDILVGIVGALIAGWL
                     FGGGASILNGPLNMMAFLYSLLGAVLLLLVVKLIRRAT"
     gene            complement(7177..>7851)
                     /locus_tag="VF547_01650"
     CDS             complement(7177..>7851)
                     /locus_tag="VF547_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261556.1"
                     /translation="AAVFSHFPLAAAGGHALRRILRDWTGPDSPRLAALRAALETAEG
                     AKPATDEGLHRRRLAAYDELYCQCGFGLAGRYPDSSLVECPEARTPLAAAAAAALRSA
                     FERHLHDLPAGSALCADLADCLGRLVGAVQTSLRAALEAQGRINALLGRSPPVRPFLG
                     RDLARFVALERPALRKEAKGPPFFVDEIADLLGIAIDIGADEVTIGPKRKTAAPPCLR
                     SPGGSA"
BASE COUNT         1198 a         2623 c         2855 g         1175 t
ORIGIN      
        1 cggcgagcac ccagttgcgc cgatcgaggg cgcgctgggt gcggcgctgg ggatcgtagt
       61 tgctgggcgc cttgggcagg atggcgagat aggccgcctc gtggagctgg agctggccga
      121 cgtccttgtc gaaataggcc cgcgaggccg cctgcacgcc ataggcgttg cggcccagga
      181 agatctggtt gaggtagagc gccaggatct gctgcttggt caggacgttc tcgatccggc
      241 gcgccaggaa ggcttccttg atcttgcggg tgaccgaata ttcgtcgccg aggaggaggt
      301 tcttggcgac ctgctgggtg atggtcgagc cgccccgggc gcgctcgccg ctgccgacct
      361 tggagatata gtcgccgacc gccccgacca ggcccggata gtcgatgccg ccgtggctga
      421 agaaggtctt gtcctcggcg gaggtgaagg cgtcgatcag ctgctgcggg aattcgtcgt
      481 aggaaagctc gacccgccgc tcgcgggcga agctctgcac cggctcgccg tcgatgccgc
      541 gcacgttggt cgggagcggc ggctggtagg tgaggagggt ttccgccgac ggcaggttgc
      601 gggcgaacag cagccagagg aggaaataga gcaggatcgg gatcgccgcg agataggcga
      661 gcctccggac ccacttcctc ccccatgcgc gccgcagcgg cccgccgaag cggcttccgg
      721 catcgcgata cagggtgaag cgggtagggg cggcgtcggc ggacattcga cggaagctct
      781 agccgatgat ggcggtgggg tgaagcgcct ccgggcttga ctttcccgat atattgtata
      841 tacaataatt atgtggaaat cgaattcgac ccggagaagc gcgaccagac gatcaaaagg
      901 cgggggttgg atttcgcaga agccgagcgg atcttcaacg tctatgcatt gaccctggaa
      961 gaccggcgat tcgattacgg cgaggaccgc ttcgttacct acggtatgct gaacggggag
     1021 ttggtcgcgt gcgtctggac gtcacgagcc gaaggacgaa cgcggcgcat catcagcttg
     1081 cggaaggcga ggaagaatga gcgagaaatc tactacctca accggccgga accccgatga
     1141 gcacgccgac gattgggatc cggacaccgc tccgatcctt acccatgagt cggcggcgcg
     1201 ggcgcaaatc tcgatcggcg acaggatcgt ccgcgaagcc gatcctccct tgggcactcg
     1261 gcgcggccgc cctcccaagc cgagcggcga acgcaaggtg ctggtgtcgt tgcggctggc
     1321 gccggacatc gtcgaatggt tccgcgccac cggtcctggc tggcaatcgc gaatcgagga
     1381 actgttgcgg cgggaggttc gcacggcggc cgagagctag cgccccgcca ccctcaccag
     1441 ccgctgcgcg aaatgggttt cgatcgcact cttcattccc agcgcgatct ggcggcggcc
     1501 ctcggccgaa tcgatatagg cggtgtcgac ggcgttggtg agatagccgg tctcgaacag
     1561 gatcgacggc acgtcgggcg ccttcagcac cacgagcgag gcgaagcggt gataatcggg
     1621 gcggaagggg aaataggccg aggcctcgcg cttcagcagc gccgcgaagt cggccgagac
     1681 gttcatgctc tcgcgctggg cgaggtcgat caggatccgg tcgacccccg cctcgccgct
     1741 gagcgccgtg ccggcgatcc ggtcggagcg gttctgctgc tcggcgagca gggccgcctc
     1801 ccggtcggac gccacttcgg acaaggtgta gatggtcgcc ccgcgggccc cgtcgttgct
     1861 cggggccgca tcggcgtgga tcgagatgaa cagctcggcg cgcatcttgc gggcgatctc
     1921 gtagcggtcc ttgaggacca ggtagcgatc gtcctcgcgg gtcatcgcca cccgcacccg
     1981 ccccgaatcg gccagcgcgt cgcggatcgc cgtggcgatg gcgagcgtca cgtccttctc
     2041 gcgcctgccg ccgaacggtg aggttgcgcc gggatcgcgg ccgccgtggc cggggtccag
     2101 cacgaccagc ggccgagccg ggtcgctcga cccgtcgatc cggggcaggc cgggagcggg
     2161 gtcgctccgg tcgatggcga tcgccccccc gtcgtcccgc ttcgccgccg gcccggacgg
     2221 agccgccgcg gcgatcagga tcagcagggc gagcagcgcc tccatcgccc taggcagccg
     2281 tgcccgcggc ccggtccgcc cagtcggcgg agcgcagccg gtagacgagg ctctcccggc
     2341 cgaaccgctc ggcgcggccg tcgagcttgc cgccgatctt ctcgaccgcc cggcgggacc
     2401 gccggttttg agggccgacg aggaacagca cgctttcgac gaagcggaag gcatggtcga
     2461 gcatcagccg cttcatctcg ccgttgaaga cgccgcccca ccggcttcgg gcgaggaagg
     2521 tccagccgat ctcgacctcg ctgcgctccg ggtcgaagcc gtgatagcgc gacgagccga
     2581 tcaccgaccc gtcggcgcgg tcgatcgcga ccagcgctcc gccggaggcc agcgcgtcct
     2641 cgaagaagcc gcggaacgcc ggctcccgcc agcggtccgg tgcgggatgc tgctcccata
     2701 tcgccgggtc ggaagcgacc gcgtagagcg cgtcccagtc tgcggggacg agcggacgaa
     2761 actcgatcag gtcgccggcg agccgaggct gggggtcgaa ttccatcgcc cgccactatc
     2821 ccggcggccg cggcgcttgg caaggcggct caccgttccg cccagcctcc gatgcgcagc
     2881 gagaccagct cggcggcgct tcgccggccg ccgccggcgg cgttgacccg gcggatgaag
     2941 tcggcactga ctccgtgggt gcgcagcttg acgatgtcgt ccgaggccag cccgccatag
     3001 ccgagcgcct tgagctcgcc gagatagtcc ggcgtgacgc catgatcgcg caggcggatc
     3061 agctcggcag tcgagaaacg atagccgtgc cgcgcggcct cgccgacgaa atcgggcatc
     3121 acgccatggt cgcgaagctg gacgagctgg tcggcgggca cgcttttcag gtcgtagccg
     3181 gccagctggc ggacgtaatc gggggtcact ccatggtcgc gcaggcggat gagctggtcg
     3241 agcgggacgt cgtagccggc ggtccggaag ccggcgacga agtccggcgt gatgccatgg
     3301 tcgcgaagcc gccccacctg ctcggacgac aggtcgcgaa ggccgaagcg ggcgagctcg
     3361 gcgacataat cgggggtgac gccgtggtcg cgcatcatga tcagggaatc gagggtgccg
     3421 acacgatagc cgtgcccgcc catgcccttc agatagtcga ggccggcgcc gtgatcgccg
     3481 gcccggatga tgtcggcgac ggtcgggcgc gcatagccct ggcgcttcag ctcctcgaga
     3541 taggcatggc cgaggtcgtg cagggcgagc gagaacagct gggcgtcggt ggcgcgcccg
     3601 tagcctcgtt ccgcgaggga agcggcgaag gccgcgctgg gctggaagcg gcactcgccg
     3661 gtgccgtgcc agcgccgcgc gacgccctcg caggcgatcg cgccggcgtc gcggttgagc
     3721 cggaagctca ccggcgcgcc ggtcggggag gcgagctgct cggcgctgag gccgtcgaga
     3781 ccggcgagcg ggcgggtgtg actgtgcatc cagcggctgt tggcggtgcg gcgggtcaga
     3841 gcgaactgga tttcgccttt cgcccgctgc cggggatcgg cctcgatcgt ccaggtcacc
     3901 tcgccttggc ctgcctcggt ctcggcgccg ggcgcggcgc aggcggcggg aatcgcggcg
     3961 ggaagcgcga aggcggcggc gaggagggcg aaaagggaag ggcgcatgct gcaactccgt
     4021 gtgggttcag ccgatcgatg caaggagcgc gccatcgcgc gaggggcgcg aatgggccgc
     4081 ttttgctcga aacgcggcgc ccttgtgtcc gcagccggac tccccggtgt ccggccgcgg
     4141 acatcggcgg cggccgatcc gcggcggctc gagcttcagg cggcgagctt gacgaagggg
     4201 acggggcggc tcggcttgag cacgatcgga cgcccgcatt cggcctggaa cagctcgccg
     4261 tcgagcagga cgttggagcc ggcgccctcg atccggatct cgtcgccccg ctcgagatgg
     4321 actccggaca ggcgctttcg gcccagcttg ccccgcaccg ccgcgaagaa ggcgccgagc
     4381 agggagccga tattgcggtc gatgacgagc agctgcatcg ctccggtgcg cgctccggcg
     4441 ccgggcatgt ggcccttgaa caggaggcgc tgcagggtcg tcaccatcag gaaggcgaac
     4501 cggccgttga gctcgccgct cttcatcccc gagactcgca gcgtcgccgg cggcggcggc
     4561 aggaagctcg ctcggcggcc gaggacgacg ccgagaaggc cgagcagaag ggccagtaca
     4621 tgggcgacgc cgttcggcag gccgagcggg tagagcttgt tccggcaata gagcatcgtg
     4681 tcggcgagat aggcgcctcc gaggaacatg ccgagcaccg gcttgccgtg gcccgaccct
     4741 cccgacagcg tgatcagctc gcgctggacg atatggggcg ctaggtcgga gcgcgcgatg
     4801 gcgaggactc gctcgagcgt ctcgagcggg tcgccgtcgg cgccgaggtc gagggcgatg
     4861 aggttcgtct tgccgttggg cagcaccgcg accgggggcg gcgaatcgcc gaaatggcgg
     4921 ccgttgtgga tctcggtcag agccgcctgg acggtgccgt cgccgccgtt gatcacaagc
     4981 acccgtggct cgaccatggc gatcgtacgc aacgccgtcc cgatctggtc gacgtgctcg
     5041 acctcgtagt ggaagatgtc cttgtgcgcc gcgcagaagc tcctgacccg cgggagcagc
     5101 gcccggttgc cggtcgaccg cgggttggag aggagcgcga cgcgggccat cgactatttc
     5161 tgcccgatcg gggcgatggt gaggccgctg cgatagttga gcacgaccgg cacgaccttc
     5221 acgcctcggc ccaccgccac cgccgccgcc cggtgcgagg gcttgagctt gagcagcgaa
     5281 agcttcgggt tgttcgagaa gccgccgccg tcgttccagt tggtcttgcc gttgttgtcg
     5341 gcgtcgtggc ggaccgcgat cgcgtaggtg cccgtgctcg gcacggcgac gcacacgttc
     5401 atcgcgccgg cgcgggtcac gggcaggtcg atccgcttca gctttgcccc gcgctcgagg
     5461 aactgggacg gattgccgcc gtaaagctgg atgcgcagct tgccgacgcg gttcttgaag
     5521 ccgttgacgc tgaccagcat ggccggccgg ttggcgccgg cgctgcaggc ggccgcgtcg
     5581 gggccgagcg ccgccgccgc cggcgcggcg ggcagggtca gcgcggccgc aacggcggca
     5641 gcggaaagaa acagtctcga catcgccact caaactcccg ggcgcttgcg ccgtgccgct
     5701 cgtcgagcgg gcagcgcggt gtacggatcc ccatcgcgag caatcgcgga gggttgcggc
     5761 caaaataggg ccgtggcggg tcgaaatgcg ttcggtggcc cgcttgacgg tttcaattgt
     5821 gacgatccgg atcgtccttc cggcgctgta acgaggtggg tttgccgcgg ggcttatcca
     5881 tgttagcgtc cccgccgggt ccaaggtcac gcaaggtccc acggcgcttc ggccgcgacg
     5941 cggccgaagc gtctcttttg attgaacgga aggggcaagg ttatgaaaat gatcgtcaag
     6001 ggcgcgatgg tcgccggtct gatggcgctc gccgcatgcg gcgaaaacac gccggccgag
     6061 aacgcggccg acaatgtcga ggcggtgagc gaaaacgtcg ccgacaacct cgaagaggcc
     6121 gccgacaaca tgtcgaacga caccgccgcc gacgcgaccg agaacaaggc cgacgccgtc
     6181 cgcgaggcgg gcgagaacaa ggccgacgcg atcgaggcca actccgccga ctgatcgccc
     6241 gggacggccg ctctcggcgc gagacgcggc cgtcccgccg attatggcga ggcgccggcg
     6301 aggcccggat ttggccgctc aggctccggt agccggaggg ctcacgtgcc tagcgacggc
     6361 cgccatctcg ccgaagcccg gctctccggc gcgcctatcg ggaaagccgc gatttggcgt
     6421 gcacccgaaa tcctccacat ccccacgcgg cgggccgtct ccgctccgcg ccggcgttcc
     6481 aaaaggcgcc gatgcacggt ctcgatcccc gagggatgaa gaatcggcgg tttgcggcgc
     6541 gtctagggtt cgcgctggcc ggcctccttc tcgtcttccg acgcgagaag agcttccgag
     6601 ctcaggccgg ccttgccgcg gccgctgtcg tcgccctcct ggtccttcgg cccggcccgc
     6661 tctgggccgc cctggtcgcc tcggccgcga cgctcgtcct cgcgctggag ctggtcaact
     6721 ccgctctcga atatctgctc gaccgcctcc accccgcgca ttgcccggag atcggcgccg
     6781 ccaaggacgc cgccgccgcc gccgtgctcg tcgcgagcct cgccgccctg ggcgtcggag
     6841 ccttgatgct gttcgccgtc ctctcgggct gacggcgcgc ggcggaacgc aatacgctcg
     6901 cgaaacgtct tcagcctgcc ggcccgagcc cgcggccgct ggagacactc gccatgaacc
     6961 tgatcatcat cctcatcgtc ggcgcggtcg tcggctggct cgccggaatc atcacccgga
     7021 gcggcggcgg gctcctgttc gacatcctcg tcggcatcgt cggcgccctg atcgccggct
     7081 ggctgttcgg gggcggcgcc tcgatcctca acgggccgct gaacatgatg gccttcctct
     7141 attcgctgct cggagccgtc ctcctgctcc tggtcgtcaa gctgatccgc cgggcgacct
     7201 gaggcacggc ggcgcggcgg tcttccgctt cggaccgatc gtcacctcgt cggctccgat
     7261 atcgatcgcg atgccgagca ggtcggcgat ctcgtcgacg aagaacggcg ggcccttcgc
     7321 ttcctttcgc aaggccggcc tctccagcgc gacgaagcgc gcgaggtccc gaccgaggaa
     7381 ggggcggacc ggtgggctgc ggccgagcag ggcgttgatc cggccctgcg cctccagcgc
     7441 cgcgcgcagc gaggtctgga cggcgccgac cagcctgccc aggcaatccg cgaggtcggc
     7501 gcacagcgcg ctgcccgccg gaaggtcgtg caggtggcgc tcgaacgccg accgcagcgc
     7561 ggccgccgcc gccgccgcca gcggcgtcct cgcctcgggg cattcgacga gcgaggaatc
     7621 cggatagcgg ccggccagtc cgaagccgca ttggcaatag agttcgtcat aggccgcgag
     7681 ccggcgacgg tgcagccctt cgtcggtggc cggcttcgcc ccttcggccg tctcgagcgc
     7741 agcgcgcagg gccgccaggc gaggcgaatc gggcccggtc cagtcgcgca gaatgcggcg
     7801 cagggcgtgg ccgccggctg ccgccagcgg gaagtgcgag aagaccgcgg c
//
LOCUS       DASVQZ010000059         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_111467, whole genome shotgun sequence.
ACCESSION   DASVQZ010000059 DASVQZ010000000
VERSION     DASVQZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111467"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VF547_01655"
     CDS             complement(<1..912)
                     /locus_tag="VF547_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX8261557.1"
                     /translation="MSLLDLPSPKTEGWRWSDLSGLPGLAARAASGAAPESLPWLPCD
                     GPRLLFVDGRLDPERSDPGPVSPGPVEAEAFDHPLARLVGGEGWALRLGRDHSPSGLV
                     QVVHVSTGGADHLAARIALDVDAHASVLETYVGEGWSNRSSAISLSRGARLMLARRVL
                     GTAGFASLTDRASVGEGASLTITTLAAGGADTRLDGEVILAGEGAYAEAGGALLARGR
                     QRHDVNLVLRHEQPLGTSRQVWRSVADDRSICSVAARVEVARHAQKTDGEQSLKGLLL
                     SRSATINAKPELEIFADDVKCAHGATVG"
     gene            complement(1022..1765)
                     /gene="sufC"
                     /locus_tag="VF547_01660"
     CDS             complement(1022..1765)
                     /gene="sufC"
                     /locus_tag="VF547_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX8261558.1"
                     /translation="MLKIENLHATVADKPILKGLTLAVNAGEIHAIMGPNGAGKSTLA
                     YTLGGRPGYEITGGSATLGGRDLLQMTPDERAAAGLFLGFQYPVEIPGVSNVQFLRTA
                     LNAQRRARGESEISAGDFLRLTREQADALGMSMDMMKRNVNVGFSGGEKKRNEMVQMG
                     IIDPRLAVLDETDSGLDIDALRIVSNGINRIMRRPDKAVLLITHYQRLLDLVVPDFVH
                     VLAEGRIVRSGGPELAHQLEREGYAEVAA"
     gene            complement(1767..>2385)
                     /locus_tag="VF547_01665"
     CDS             complement(1767..>2385)
                     /locus_tag="VF547_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX8261559.1"
                     /translation="GIEMGETAGADRRAPAHGEGEERPVKSPYQDKGWRPEAPPLQGR
                     GLGWGLSNAQLKELHRRAAEMRRNPTEPEKRLWRNLSNSQLGDHKFRRQSVIGCFIAD
                     FLCPQKALIVEVDGDTHDEAKDRVRDDILAKRGFRVLRVTNDDVMSNMDGVLQHILIT
                     LNETPGRWNNPHPNPSPEGEGLEEVHPSPSGDGPGVGPIRKESVR"
BASE COUNT          372 a          787 c          836 g          390 t
ORIGIN      
        1 gccgaccgtc gctccgtgag cgcatttgac gtcgtcggcg aagatctcga gctccggctt
       61 ggcgttgatc gtcgcggagc gggagaggag gagacccttc agcgactgct cgccgtccgt
      121 cttctgggcg tggcgggcga cctcgacccg ggcggcgacc gagcagatcg accggtcgtc
      181 cgcgaccgat cgccagacct ggcggctggt gccaagcggc tgctcgtggc ggaggacgag
      241 attgacgtca tggcgctgcc gtccgcgggc gagaagggcg ccgcccgcct cggcataggc
      301 gccctcgccg gcaaggatca cctcaccgtc gagcctcgtg tcggcgccgc ctgcggccaa
      361 ggtggtgatc gtcaggctgg cgccttcgcc gacgctggcg cggtccgtca ggctggcgaa
      421 accggcggtt ccaaggaccc ggcgagccag catcagccgg gcgcccctgg acaggcttat
      481 cgcggacgac cggttggacc agccttcgcc gacataggtt tcgagcaccg aggcgtgggc
      541 gtcgacgtcc agcgcgatcc gagccgcgag atggtccgcg ccgccggtcg agacatgaac
      601 gacctggacg aggcccgaag gcgaatggtc gcggccgagc cggagagccc agccctcgcc
      661 tccgacgagc cgcgccagag ggtggtcgaa agcttccgcc tcgaccgggc cgggcgacac
      721 ggggcccgga tcgcttcgct ccggatccag ccggccgtcg acgaagagca ggcgcggccc
      781 gtcgcagggg agccagggaa gcgactcggg agcggccccg gatgccgccc gagccgcgag
      841 cccgggcagg ccggaaagat cgctccagcg ccagccctcg gtcttcggcg atggaaggtc
      901 gagaaggctc acggcgccgc ctttccctgg atcgggcgac tgtccggaac cggacgaaga
      961 tccgtaaggt cagtgtcaca cgcgcgcgcg cgcgagggga ggagcaagcg acgatcccgc
     1021 gtcatgccgc cacctcggca tagccttcgc gctcgagctg gtgagcgagc tccggcccgc
     1081 cggagcggac gatccgccct tcggccagca cgtgcacgaa gtccgggacg acgaggtcga
     1141 ggaggcgctg ataatgggtg atcaggagca ccgccttgtc ggggcggcgc atgatccggt
     1201 tgatcccgtt cgagacgatt cgaagcgcgt cgatgtcgag gccggaatcg gtttcgtcga
     1261 gaaccgccag gcgcggatcg atgatcccca tctggaccat ctcgttgcgc ttcttctcgc
     1321 cgccggagaa gccgacgttc acgttgcgct tcatcatgtc catcgacatt cccagcgcat
     1381 ccgcttgctc gcgggtcagg cgaaggaagt cgcccgccga aatctccgac tcgcctcggg
     1441 ctcggcgctg cgcgttgagc gcggtgcgga ggaactggac gttggaaacg ccgggaatct
     1501 cgaccggata ttggaagccg aggaacaggc ccgcggcggc gcgctcgtcc ggcgtcattt
     1561 ggagcaggtc gcggccgccc agggtcgccg agccgcccgt aatctcatag ccgggccgtc
     1621 cgccgagcgt gtaggccagc gtcgacttgc ccgcgccgtt cggacccatg atggcgtgga
     1681 tctcgccggc gttcaccgcg agggtaaggc ccttgaggat cggcttgtcg gcgaccgtgg
     1741 cgtggaggtt ttcaatcttc agcattctaa cgaacactct cttttcggat aggccccacc
     1801 cccggcccgt cccctgaagg ggaggggtga acttcctcta gcccctcccc ttcaggggag
     1861 gggttggggt gggggttgtt ccagcgaccc ggcgtttcgt tcaaggtgat caggatgtgc
     1921 tggaggacgc catccatgtt ggacatcacg tcgtcgttgg tcacgcgcag gacgcggaaa
     1981 ccgcgcttgg cgagtatgtc gtcgcgcacg cgatccttgg cctcgtcgtg cgtgtcgccg
     2041 tcgacttcga cgatcagcgc cttctgcggg cagaggaaat cggcgatgaa gcagccgatc
     2101 accgactggc ggcgaaattt atggtcgccg agctgagagt tggacaggtt ccgccacagt
     2161 cgcttctcgg gctcggtggg attgcggcgc atctccgcgg cgcggcggtg gagctccttc
     2221 agctgcgcgt tcgacaggcc ccaccccaaa cccctcccct gaaggggagg ggcttcaggg
     2281 cgccagcctt tgtcctggta aggagacttc actggacgct cctctccttc gccatgcgcc
     2341 ggcgctcggc gatctgcgcc ggcagtttcg cccatctcga ttcca
//
LOCUS       DASVQZ010000060         3090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_207650, whole genome shotgun sequence.
ACCESSION   DASVQZ010000060 DASVQZ010000000
VERSION     DASVQZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207650"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1118
                     /locus_tag="VF547_01670"
     CDS             258..1118
                     /locus_tag="VF547_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8261560.1"
                     /translation="MLIVNAHSRRGRELFREAVEKLRAAGVELTASYAIRDPAKLVPK
                     VREAVRDGAPMVIVGGGDGSLSCAVDELVDRDCVFALLPLGTANSFARTLGIPLDLDG
                     AIAVIAEGRRRRVDLGMIGDDYFANCAAMGLSPMIGEGVPHKLKRWLGRVGYLIWAIW
                     CLVRFHPFRLTVDDGSGPRTIWATEVRIANGGFHGGVEVVEAADVDSGEIVIQAVTGR
                     SNLLLIWDWISKYLKLPARHATTEEFHGRRFTLDTRPRQRISIDGEVSARTPVTVQVA
                     ERAIEVVVPA"
     gene            complement(1287..2747)
                     /locus_tag="VF547_01675"
     CDS             complement(1287..2747)
                     /locus_tag="VF547_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8261561.1"
                     /translation="MNSRAGPRGGRDLTQGPIAPTLLAFALPTLGSSILQSLNGSINA
                     IWVGRFLGEEALAATSNANIVIFLFTAFVFGFSMAATILVGQAFGRGDVDGARRAIGT
                     ATGAFVPISILGAAAGWLLAPAILKLLGTPADAAPLALAYLRVIFIAMPPALIMVSWM
                     MALRGAGDSLTPLWFMVLAVLLDSGLNPVFILGLGPAPRLGIAGAATATVIANCVSLA
                     GLLVHVYVRDLPLRLRGAELRYLKPDRALLATVVRKGLPMGLQMIVVSASALALIGLV
                     NREGVHTAAAFGVAMQLWTYLQMPAMALGAAVSAMGAQNLGAGRWDRVGAITRSGVVF
                     AVSITAGLVLLLAIADRPALALFLGGDSPALPIARHIQILATWSFLLFGVTLVLFGTV
                     RANGAVIGPLVILFVGLFPVRLGFALGAYPWLGADALWLSFPVSSLVNMGLASAFYLH
                     GGWRSSRMQAPPVAADECVEEALADAEPGGRLNPAG"
     gene            complement(2875..>3090)
                     /locus_tag="VF547_01680"
     CDS             complement(2875..>3090)
                     /locus_tag="VF547_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
                     /protein_id="HEX8261562.1"
                     /translation="LEGVIDVDAERARLSKAAEAAEKERDSLAARLANPSFVERAKPE
                     AVEKARADHEAKASDAEKYRAALARLG"
BASE COUNT          502 a         1071 c         1113 g          404 t
ORIGIN      
        1 atccacgacc gccagaccaa caagaagtac aagtccaacc tggccgacac gaccttgtag
       61 gggaattcct ccccggcatt ggccggggac ggggaccggc cgaaggctgg tgggggtctt
      121 tcaggcaccg aagaacccct ccaccgcgct tcgcgcggtc cccctccccc ggaaatccgg
      181 gggaggaatt aatcggacct tcccccttct ccctgcgttg cctcaccgtg tccccgagtc
      241 ccgttcccaa acaggccgtg ctgatcgtca acgcccattc gcggcgcggg cgggagctgt
      301 tccgcgaggc cgtcgagaag cttcgcgccg cgggcgtcga gctcaccgcc tcctatgcga
      361 tccgcgaccc ggccaagctc gtgcccaagg tccgcgaggc ggtgcgggac ggcgctccga
      421 tggtcatcgt cggcggcggc gacggctcgc tctcctgcgc cgtcgacgag ctggtcgacc
      481 gcgactgcgt cttcgcgctg ctgccgctgg gcaccgccaa cagcttcgcc cggacgctcg
      541 gaatcccgct cgacctcgac ggggcgatcg ccgtcatcgc cgagggccgc cggcgccggg
      601 tcgacctcgg catgatcggc gacgactatt tcgccaattg cgcggcgatg ggcctgtcgc
      661 caatgatcgg cgagggcgtc ccgcacaagc tgaagcgctg gctcgggcgg gtcggctatc
      721 tgatctgggc gatctggtgc ctggtccggt tccacccctt ccggctgacc gtcgacgacg
      781 gcagcggccc gcgcacgatc tgggcgaccg aggtccggat cgccaacggc ggcttccacg
      841 gcggagtcga ggtggtcgag gccgccgatg tcgacagcgg cgagatcgtc atccaggcgg
      901 tgacggggcg aagcaacctg ctgctgatct gggactggat ctcgaaatat ctcaagcttc
      961 cggcccgcca cgccaccacc gaggaattcc acggccgccg cttcacgctc gacacccgcc
     1021 cgcgccagcg catctcgatc gacggcgagg tgagcgctcg aacgccggtc accgtccagg
     1081 tcgccgagcg cgcgatcgag gtcgtcgtcc cggcctgatc cggcgaaggc cgccgaaaca
     1141 taaaattcct tccgatcggt cccggccgat cggagcacct tcgaggcaag agccgtcgtc
     1201 ccagcgcagg ctgggaccca agggagagtt cgcactgccg tccgggccgg gggcgttcag
     1261 gcggcgggga tccttcgccc cctgggctag ccggcgggat tgagacggcc gccgggctcg
     1321 gcgtcggcca gggcctcctc gacgcattcg tcggccgcga ccgggggcgc ctgcatccgg
     1381 ctcgagcgcc agccgccgtg aagatagaaa gccgaggcga ggcccatgtt gacgagcgag
     1441 ctgaccggga agctgagcca cagggcgtcg gcgccgagcc agggataggc gccgagcgcg
     1501 aagccgaggc ggaccggaaa cagcccgacg aacaggatga cgagcggacc gatcaccgcg
     1561 ccgttcgccc gaaccgtgcc gaacaggacg agggtcactc cgaacagcag gaagctccac
     1621 gtggcgagga tctggatgtg gcgggcgatc ggcagggccg ggctgtcgcc gccgaggaac
     1681 agggccaggg cgggccggtc ggcgatggcg aggaggagga cgaggccggc ggtgatcgag
     1741 acggcgaaga cgacccccga gcgggtgatc gcgcccaccc ggtcccagcg gccggcgccg
     1801 agattctggg cgcccatcgc gctgaccgcg gcgccgagcg ccatggccgg catctgcaga
     1861 taggtccaga gctgcatcgc cacgccgaag gcggcggcgg tgtggacgcc ctcgcggttg
     1921 acgaggccga tcagggcgag cgccgaggcg gagacgacga tcatctggag gcccatcggc
     1981 aggcccttgc gaacgacggt cgcgagcagc gcccggtccg gcttcaggta gcgcagctcg
     2041 gccccgcgca gcctgagcgg caggtcgcgg acgtagacat ggacgagcaa gccggcgagg
     2101 ctgacgcaat tggcgatcac cgtcgcggtg gcggcgccgg cgatgcccag cctcggcgcc
     2161 gggccgaggc cgaggatgaa gaccgggttg aggccgctgt cgagcaggac ggccaggacc
     2221 atgaaccaca agggcgtcag ggagtcgccc gcgcccctca gcgccatcat ccacgacacc
     2281 atgatcaagg cgggcggcat ggcgatgaag atgacccgca ggtaggcgag cgcgagcggc
     2341 gcggcgtcgg ccggcgttcc gagcagcttg aggatcgccg gcgcgagaag ccagcccgcg
     2401 gcggcgccga ggatcgagat ggggacgaac gctccggtcg cggtgccgat cgcccgccgc
     2461 gccccgtcga cgtcgccgcg cccgaacgcc tggccgacga ggatcgtcgc cgccatgctg
     2521 aagccgaaga cgaaggcggt gaagaggaag atgacgatat tggcgttgga cgtcgcggcg
     2581 agcgcctcct cgccgaggaa gcgcccgacc cagatcgcgt tgatcgagcc gttgagcgac
     2641 tggaggatgg acgagccgag cgtcggcaaa gcgaaggcga gcagggtcgg cgcgatcggc
     2701 ccttgggtga ggtcgcggcc gccgcggggt cctgctcgag agttcatcgc ctgctcccgt
     2761 tcaccccctc tccccggcgg ggagagggtc ggggtgaggg gtcacggcgc ctgtcgctcg
     2821 agcgccgaac cccctcaccc cctgccactc tcccccaagg ggagagggga gaattcaacc
     2881 cagccgggcc agggccgcgc gatacttctc cgcgtccgag gccttcgcct catggtcggc
     2941 ccgagccttc tccaccgctt ccggcttcgc ccgttcgacg aagctcggat tggcgaggcg
     3001 cgcggcgagg gagtcccgct ccttctcggc cgcctcggcg gccttcgaaa gccgagcccg
     3061 ctcggcatcg acgtcgatga cgccctcgag
//
LOCUS       DASVQZ010000061         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_399645, whole genome shotgun sequence.
ACCESSION   DASVQZ010000061 DASVQZ010000000
VERSION     DASVQZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399645"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF547_01685"
     CDS             complement(<1..576)
                     /locus_tag="VF547_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8261563.1"
                     /translation="MTDIRNREAQDAVDRASTYEWGFTSEIEQDFAPKGLGEDTVRFI
                     SAKKNEPEWMLEWRLKAYRAWLTMEEVTWAKLDIPPIDFQDAYYYAEPRQKPTLASLD
                     ELDPEIRRTYEKLGIPIEEQKVLAGVEGARKVAVDAVFDSVSVATTFREELKRAGVIF
                     LSISEAVREYPDLVRKYLGSVVPQRDNYFACL"
     gene            complement(573..1043)
                     /locus_tag="VF547_01690"
     CDS             complement(573..1043)
                     /locus_tag="VF547_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEX8261564.1"
                     /translation="MRLTSLADYAVVVMAAAARRPAGARLSASLLAEETGVPLPTTQK
                     VMGRLAGAGLLESARGTGGGFRLARAAAVISLADIIEAVEGPIAMTNCIEGSIHDCAL
                     EGSCRVKPHLNAANDAVRSALAAVSLAQLCAPAKAGAQIKAPDWAPASAGEQVR"
     gene            1157..1639
                     /locus_tag="VF547_01695"
     CDS             1157..1639
                     /locus_tag="VF547_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261565.1"
                     /translation="MRPVPFTMLLAALLLPAAACGEPPPAAPRPGDLYELTKSYETSM
                     ETSDGSSGSSQGQDALLERVIAVRPDGLELEYDVPKSDSSGAYWQFPVRVFRPVGGEL
                     QLLNRAELESRVDGWLKKGKMTAPPADAGSSPGTRSGSTATRNRRSTSSPPTSSGRRI
                     "
     gene            1735..2025
                     /locus_tag="VF547_01700"
     CDS             1735..2025
                     /locus_tag="VF547_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261566.1"
                     /translation="MEVDPDVVRRGEAESDVVVAELSGKPVTIDAAARERAKQSVSGT
                     ISVTLHADAAGNVTKRTKTVVLKVRKPDGPLEKRTATETVERRFVSGRGGGR"
     gene            2098..3117
                     /locus_tag="VF547_01705"
     CDS             2098..3117
                     /locus_tag="VF547_01705"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX8261567.1"
                     /translation="MALYPLVRPLVFALDAERAHRLTIRALRAVPAGEPAREDPRLAI
                     RVAGLDFPNPVGLAAGFDKDAEVPAQMLGFGFGFVEVGTLTPRPQPGNERPRLFRLAE
                     DRAVVNRMGFNNGGFDAAKARLERRRRGIVGVNVGANKDSADRIADYVAGVGELGPFA
                     DYLTINVSSPNTPGLRGLQDEGALRALLAAVGEAREGVAPIFLKVAPDLGPGEIDGIA
                     RAAVEAGIDALIVGNTTLSRPPLRSRHGREAGGLSGAPLKPLALQRLKDFRAATGAGI
                     PLIAAGGIENGVDAYERIRAGASLVQLYTALVYQGPGLAAAILGELRALLSRDGFETL
                     AEAVG"
     gene            complement(3149..>3859)
                     /locus_tag="VF547_01710"
     CDS             complement(3149..>3859)
                     /locus_tag="VF547_01710"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8261568.1"
                     /translation="VRVPVAGLLDPAYVDSRARLIGDRAGPAPKAGAPAGAPVAARDS
                     TLEPAGTTHFIVRDAAGNVVSMTATVESLFGSGRMVDGFFLNNEMTDFAFDPRDSEGR
                     PAANAVAPGKRPRSSMTPLVMLDADRRFAGALGSAGGNAILAYVGKSLVAAVDWNLAM
                     GEALALPNLVARGGAFQGEVTRFEPAVLQGLRARGIELRPGQGEDSGLTGVIVRAGRL
                     DGAADPRREGVARIERAR"
BASE COUNT          548 a         1332 c         1428 g          551 t
ORIGIN      
        1 caggcaggcg aagtaattgt cgcgctgcgg gaccaccgag ccgagatatt tgcggaccag
       61 gtccggatat tcgcggacgg cctcggagat ggagaggaag atgaccccgg cgcgcttcag
      121 ctcctcgcgg aaggtggtgg cgacggagac gctatcgaac acggcgtcga ccgccacttt
      181 gcgggcgccc tcgaccccgg cgagcacctt ctgctcctcg atcgggatcc ccagcttctc
      241 gtaggtgcgg cggatctcgg ggtcgagctc gtcgagcgag gcgagggtcg gcttctggcg
      301 cggctcggcg taataatagg cgtcctggaa atcgatcggc gggatgtcga gcttggccca
      361 ggtcacctcc tccatcgtca gccaggcccg atacgccttc aatcgccatt cgagcatcca
      421 ctcgggctcg ttcttcttcg ccgagatgaa gcggaccgtg tcctcgccga ggcccttggg
      481 cgcgaaatcc tgctcgatct cggaggtgaa gccccattcg taggtcgacg cccggtcgac
      541 cgcgtcctgc gcctcgcggt tgcggatgtc ggtcatcgca cctgctcccc ggcggaagcc
      601 ggggcccagt cgggcgcctt gatctgggcc ccggccttcg ccggggcgca gagctgcgcc
      661 agcgacacgg ccgcgagcgc gctgcgcacg gcgtcgttgg ccgcgttgag atggggcttg
      721 acccggcagc tgccctcgag cgcgcaatcg tggatcgatc cttcgatgca gttggtcatc
      781 gcgatcggtc cctcgaccgc ctcgatgatg tcggcgaggc tgatgacggc cgccgcccgc
      841 gcgagtcgaa agcccccgcc ggtgccgcgg gccgattcga gcagaccggc gccggcgagc
      901 cgtcccatca ccttctgggt ggtcggcagc ggcactccgg tctcctccgc gaggaggctc
      961 gccgacaggc gcgcgcccgc cggccgccgg gcggcggcgg ccatcacgac gacggcgtag
     1021 tcggcaaggg aggtgaggcg catgttctaa tcggaccaaa tcgttccgat tgcatgtggc
     1081 ggggttgcgg ccgggaatca agcctttgct cccctcgcac gttgcaaggg cggcattaag
     1141 cgggcagtcc cgggagatga gaccggtccc cttcacgatg cttctcgccg ccctgctgct
     1201 gccggccgcc gcctgcggcg aaccgccgcc cgccgccccg cggcccggcg acctctatga
     1261 gctgaccaag agctacgaaa cctcgatgga gaccagcgac ggctcgtcgg gaagctcgca
     1321 gggccaggac gcgctcctcg agcgcgtgat cgcggtgcgg cccgacggcc tggagctgga
     1381 atatgacgtc cccaagtcgg actcgagcgg cgcctattgg cagtttccgg tgcgcgtctt
     1441 ccggccggtc ggcggagagc tgcaattgct caaccgggcc gagctcgaat cgcgggtcga
     1501 cggctggctg aagaagggca agatgaccgc gccgcctgcg gacgctggat cttcacctgg
     1561 gacgcgttcc ggatcgactg cgacccgcaa tcggcgatcg acatcatctc cgcctacgag
     1621 ctcgggccgt cggatctgag ggacggcgct ccctatcgga cgcgcgaggc ccgggcgccg
     1681 gcgccgctgg cgaggaaggc cgcagggccg gagggctcga cctatgtcgc ggagatggag
     1741 gtcgaccccg acgtggtccg ccgcggcgag gcggaatccg acgtcgtcgt ggcggagctc
     1801 tcgggcaagc ccgtcaccat cgacgccgcg gcccgggagc gggcgaagca gtcggtttcc
     1861 ggaacgatca gcgtcaccct gcatgccgat gcggcgggga acgtcacgaa gcggaccaag
     1921 acggtcgtgc tgaaggtgcg caagccggac ggccccttgg agaagcggac ggccaccgag
     1981 accgtcgagc ggcggttcgt ctcggggcgc gggggcggac gatagcgacg gcgcggcgct
     2041 ctcccggggc tccgaaacgg gttcaggatc gggggaggct tatgccatag gcccgccatg
     2101 gcgctttacc ccctcgtccg gccgctcgtc ttcgccctcg acgcggagcg ggcgcaccgc
     2161 ttgacgattc gggcgctgag ggccgtgccg gcgggcgagc cggcgcggga ggatccgcgc
     2221 ctcgcgatcc gagtcgccgg gctcgatttc ccgaacccgg tcggtctcgc cgccggcttc
     2281 gacaaggacg ccgaggtgcc cgcccagatg ctcggcttcg gtttcggctt cgtcgaagtg
     2341 ggcaccctca cgccgcgccc gcagccgggc aatgagcggc cccgcctgtt ccggctcgcc
     2401 gaggatcggg cggtggtcaa ccggatgggc ttcaacaacg gcggcttcga cgcggccaag
     2461 gcccggctgg agcgccgtcg gcgcggcatc gtcggagtca atgtcggcgc caacaaggac
     2521 agcgccgacc gaatcgccga ctatgtcgcg ggggtgggcg agctcggacc gttcgccgat
     2581 tatctcacca tcaacgtctc ctcgcccaac acgccgggcc tgcggggact gcaggacgag
     2641 ggcgcgctcc gggcgctgct cgcggccgtc ggcgaggccc gcgaaggcgt cgcgccgatc
     2701 ttcctgaagg tggccccgga cctcgggccg ggggagatcg acggcatcgc ccgcgccgcc
     2761 gtcgaggccg ggatcgatgc cctgatcgtc ggcaatacga cgctgtcgag gccgccgctt
     2821 cgctcgaggc acggccgcga ggccgggggg ttgtcgggag cgccgctcaa gccgctcgcc
     2881 cttcagcggc tgaaggattt ccgggccgcc accggcgccg gcatccccct gatcgccgcc
     2941 ggggggatcg agaacggggt cgacgcctat gagcgcatcc gcgccggcgc cagcctcgtc
     3001 cagctctata cggcgctggt ctaccagggc cccggcctgg ccgcggcgat cctcggcgag
     3061 ctcagggccc tgctgagccg cgacgggttc gagaccctcg ccgaagcggt gggatgaagg
     3121 cccgatcccg ttgcccgggc cggtgcggtc agcgggctcg ctcgatccgc gcgacgccct
     3181 cgcgtcgcgg gtcggcggcg ccgtcgaggc ggccggcccg gacgatgact ccggtcagcc
     3241 ccgaatcctc gccctggccg ggccgaagct cgatgccgcg ggcgcgcagg ccttggagga
     3301 cggcgggctc gaaccgggtg acttcgccct ggaaggcgcc gccccgggcg acgaggttgg
     3361 gcagggccag ggcttcgccc atggcgaggt tccagtcgac ggcggcgacc agcgacttgc
     3421 cgacataggc gaggatggcg ttgccgccgg ccgagccaag ggcgccggcg aagcggcggt
     3481 cggcgtccag catgacgagc ggagtcatgg acgagcgcgg ccgcttgccc ggggcgacgg
     3541 cattggcggc cgggcgcccc tcggagtcgc gcgggtcgaa ggcgaagtcc gtcatctcgt
     3601 tgttgaggaa gaagccgtcg accatccgcc ccgagccgaa cagggattcg acggtcgcgg
     3661 tcatcgagac gacgttgccg gccgcgtcgc ggacgatgaa atgggtggtg ccggccggct
     3721 ccagggtgga atcgcgcgcg gcgacggggg ccccggccgg cgctcccgcc ttcggcgccg
     3781 ggccggcgcg gtcgccgatc agccgcgccc gggagtcgac ataggcgggg tcgagcaggc
     3841 ccgcgaccgg cacccggac
//
LOCUS       DASVQZ010000062         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_880318, whole genome shotgun sequence.
ACCESSION   DASVQZ010000062 DASVQZ010000000
VERSION     DASVQZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880318"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1344)
                     /locus_tag="VF547_01715"
     CDS             complement(31..1344)
                     /locus_tag="VF547_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8261569.1"
                     /translation="MRFRFLACLSLLAAVPAAAETVVTGARIRGPAEPTTIVVHAGRL
                     VTDASRPAVGPSTITITDGRITAVTDGLTPPPAGAGFVDLSGKTVLPGLIDMHVHLSG
                     DPGGDFRDEAVDPDEWAALVGAKNARLTALAGFTTVRDLGSPPLVGFMLRRATDRGFV
                     PGPRIVASGPAISIVGGHGDVSGFRPEVIRALGVDNTCTGAEECAARVREFSRAGADI
                     IKITATGGVLSQQARGLGQHFTDAEMRSIVDTAHSLGLKVAAHAHADAGIAAAVRAGV
                     DSIEHGTFASAATIRLMKERGTSLVPTLMAFTGIRERLGKGIYTPVVETKIRETLSEV
                     GKAARLARAAGVPVVFGTDAAVFEHGRNAEELAMLVELAGMSPAEAIASATSGAARLL
                     GMETQIGRIAPGYSADLIAVSGDPLRDVRALEHVDFVMVRGRTIR"
     gene            complement(1460..2383)
                     /locus_tag="VF547_01720"
     CDS             complement(1460..2383)
                     /locus_tag="VF547_01720"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8261570.1"
                     /translation="MRKALIGILMAASAAVPAVAQAQDSDRDARREARAEQARERYQA
                     AGERNQARYERRVERQEARQEERQVQRQQRAEAPVQVQQAAPVQQQQQRQSWSDRRGG
                     DGQRSERWSGGQRGERWGGGDRSDYRRDIEASREASRRSADQTIGGNYARQAQRNQER
                     YEQRVREDRHDRDGGWDRDRRDRDGGWDRDRRGDRWSDNRGSRRGSWNRSWRNDNRYD
                     WQRYRYSNRNLFRSGRYYAPYRGYSYNRLNIGFILDSLFYSNRYWLSDPYQYRLPPAP
                     YGTQWVRYYDDVVLVDVYTGEVLDVIYDFFW"
     gene            complement(2489..3031)
                     /locus_tag="VF547_01725"
     CDS             complement(2489..3031)
                     /locus_tag="VF547_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8261571.1"
                     /translation="MSRFTVNGQPVHYRLDPETPLLWALRDASNLTGTKYGCGTGHCG
                     ACTVHVDGRAVRSCRVRLADLEGSFVTTIEALSNDRSHPVQQAFVAESVPQCGFCIPG
                     MVMAAAALIDRNAAPTDAEIDSAITNICRCGIYPRVREAVRRAGRMRSGEGRITAAPP
                     PAIDPADAARQVPALRPRRP"
     gene            3064..>3614
                     /locus_tag="VF547_01730"
     CDS             3064..>3614
                     /locus_tag="VF547_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8261572.1"
                     /translation="MGSERISIGRDGAVAHVELARDGKFNAMDHDMFAAIGEAFRTLG
                     ADPSVRAILLSGRGRHFTAGLDLHYASSQFPPSEDPGRAAEARLRHIKWLQDAFTAAE
                     EARPPVIAAIHGGCIGAGVDLATACDIRLASADAFFQVAEVDVAITADLGTLQRLAHL
                     IPEGIVRELAYTGRRMGAEEAARL"
BASE COUNT          498 a         1243 c         1324 g          549 t
ORIGIN      
        1 gggcgccggg gtcgccgatc gcgcgcgtgc tcatcggatc gttcttcccc ggaccatcac
       61 gaagtcgaca tgctccaggg cgcgcacatc gcgcaagggg tcgccgctca ccgcgatcag
      121 atcggccgaa tagcccgggg cgatgcgtcc gatctgggtc tccatcccga gcagcctggc
      181 ggcgccgctg gtcgccgagg cgatcgcctc ggccgggctc atgccggcga gctcgaccag
      241 catcgccagc tcctcggcat tgcggccgtg ctcgaacacc gcggcgtcgg tcccgaacac
      301 gaccggcact ccggcggccc gggcgagcct ggccgccttg cccacctcgc tcagggtctc
      361 gcggatcttg gtttcgacga ccggcgtgta gatgcccttg ccgagccgct ctcggatgcc
      421 ggtgaacgcc atcagggtgg gaaccagcga ggtcccccgc tccttcatca gccggatggt
      481 ggcggcgctg gcgaaggtgc cgtgctcgat cgagtcgacc ccggcgcgga cggcggcggc
      541 gatgccggca tcggcatggg cgtgcgcggc gaccttgagc ccgagcgaat gggcggtgtc
      601 gacgatcgac ctcatctcgg cgtcggtgaa atgctggccg aggccgcgcg cctgctgcga
      661 gaggactccg ccggtcgccg tgatcttgat gatgtcggcg ccggcgcggg agaattcgcg
      721 gacccgggcg gcgcattcct cggcgccggt gcaggtgttg tcgactccga gcgctcggat
      781 cacctcggga cggaagcccg agacgtcgcc gtggccgccg acgatcgaga tggcggggcc
      841 cgaagcgacg atccgcgggc cgggaacgaa gccccggtcg gtggcgcggc gaagcatgaa
      901 gccgaccagg ggcggcgagc cgaggtcgcg gaccgtcgtg aagccggcga gcgcggtgag
      961 gcgcgcgttc ttggcgccga cgagggcggc ccattcgtcc ggatcgacgg cctcgtcgcg
     1021 gaaatcgccg ccggggtcgc cggagaggtg gacgtgcatg tcgatcagtc cgggcaggac
     1081 cgtcttgccc gacagatcga cgaacccggc tccggcgggc ggcggcgtca ggccgtcggt
     1141 gacggcggtg attcggccgt cggtgatggt gatcgtcgaa gggcccacgg ccggtctgga
     1201 cgcgtcggtg acgaggcggc cggcgtggac gacgatcgtc gtcggctcgg cggggccgcg
     1261 gatccgggcg ccggtgacga cggtctcggc ggcggccggc acggcggcga gaaggcttag
     1321 gcaggctagg aagcggaagc gcatcgcgaa ccccttggcg agagggcgcc tttctgctga
     1381 tccgccaagg cgttgacaag caaaaagccc ggagcgccat cgcgctccgg gctccttgtc
     1441 cgtcggcttc gtccggttcc taccagaaga agtcgtagat cacgtcgagc acctcgccgg
     1501 tgtagacgtc gaccagcacc acgtcgtcat aatagcggac ccactgggtg ccgtagggcg
     1561 ccggcggcag gcggtactga tacggatcgc tcagccagta gcggttcgaa tagaacaggg
     1621 aatcgaggat gaagccgatg ttcagccggt tgtagctgta gccgcggtag ggcgcgtaat
     1681 agcggcccga gcggaagagg ttgcggttcg aatagcggta gcgctgccag tcgtaccgat
     1741 tgtcgttgcg ccagctgcgg ttccagctgc cgcggcggct gccgcgattg tcgctccagc
     1801 ggtcgccgcg gcgatcgcgg tcccagccgc cgtcgcggtc gcggcgatcg cggtcccagc
     1861 cgccgtcgcg gtcgtgccgg tcctcgcgga cgcgctgctc gtagcgctcc tggttgcgct
     1921 gcgcctggcg ggcataattg ccgccgatcg tctggtcggc gctccgccgc gaagcctcgc
     1981 ggctcgcctc gatgtcgcgc cgatagtcgg aacggtcgcc gccgccccag cgctcgccgc
     2041 gctggccgcc gctccagcgc tcgctgcgct ggccgtcgcc gccgcgccgg tccgaccagg
     2101 actgccgctg ctgctgctgc tgaacgggcg cagcctgctg gacctgcacc ggcgcctcgg
     2161 cgcgctgctg acgctggacc tggcgctctt cctgccgcgc ctcctgacgc tcgacccggc
     2221 gctcgtagcg cgcctggttg cgctcgccgg ccgcctgata acgctcgcgc gcctgctcgg
     2281 cccgagcttc gcgccgagcg tcgcggtcgc tgtcctgggc ctgcgccacc gcgggcaccg
     2341 ccgccgaagc cgccatcaaa atcccgatta atgccttacg catggagaac tcctttcacc
     2401 ccggccggcc gctctcggcg ctcccgatcg ccattcgctt tgccgattcg aggatgagcc
     2461 gttgctgaac cgtgacgaag ggttccgttc agggtctgcg cgggcgcagg gcgggaacct
     2521 ggcgggccgc gtcggccgga tcgatggcgg ggggcggcgc ggcggtgatc cggccttcgc
     2581 cgctgcgcat ccggccggcg cggcgcacgg cctcgcggac ccgaggatag atcccgcagc
     2641 ggcagatgtt ggtgatcgcc gagtcgatct cggcatcggt gggggcggcg ttgcggtcga
     2701 tcagcgcggc ggcggccatg accatgcccg ggatgcagaa cccgcattgc ggcacgcttt
     2761 ccgcgacgaa ggcctgctgg acggggtgcg agcggtcgtt cgaaagcgcc tcgatggtgg
     2821 tgacgaagct cccctcgaga tcggcgaggc gaacgcggca gctcctcacc gcgcgaccgt
     2881 cgacatggac ggtgcaggcg ccgcaatgac cggttccgca gccgtatttg gtgccggtga
     2941 ggttggacgc gtcgcgcagc gcccaaagca ggggcgtctc agggtcgagg cggtagtgga
     3001 cgggctgccc gttgacggta aagcggctca tatcacgccg tttctagggg agcccgacgg
     3061 aagatgggaa gcgaaaggat ctcgatcggc cgcgacggcg ccgtcgccca tgtcgaactg
     3121 gcccgggacg gcaagttcaa cgccatggac catgacatgt tcgccgccat cggcgaggcg
     3181 ttccggaccc tcggcgccga tccttcggtg cgggcgatcc tcctgtccgg ccgcgggcgc
     3241 cacttcaccg ccggcctcga ccttcactat gcttcgagcc agttcccgcc gagcgaggat
     3301 ccaggccgcg cggccgaggc gcgcctcagg cacatcaagt ggcttcagga cgccttcacc
     3361 gcggcggagg aggcgcggcc gccggtgatc gcggcgatcc acggcggctg cattggcgcc
     3421 ggcgtcgacc tggccacggc ctgcgacatc cgactcgcct cggccgacgc cttcttccag
     3481 gtcgcggagg tcgacgtcgc catcaccgcc gacctgggca cgctccagcg cctcgcccac
     3541 ctcatcccgg aagggatcgt ccgcgagctc gcctataccg gccgccggat gggggccgag
     3601 gaggcggcca ggct
//
LOCUS       DASVQZ010000063         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_783403, whole genome shotgun sequence.
ACCESSION   DASVQZ010000063 DASVQZ010000000
VERSION     DASVQZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783403"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VF547_01735"
     CDS             complement(<1..896)
                     /locus_tag="VF547_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261573.1"
                     /translation="MIINDRLAAWLESPSARKATAEAMAELSARIASIPAFAALGREL
                     AAAAAAGSDAVLALAGAFIGDDGAIQAVIDASLAAAARDPLCRPPLRASRNEVQDGLL
                     LFSHPLLVVQLAVMGADALAIKRGSDVDAPPIAFTGQRMLFRFLKGGNAVLSIWEAPM
                     IGSGFTAKAGARCRLRERRRLADGDSIAFDGRRESFVVERAESDLVYIFASTSLDSSP
                     VATEYDPDSLELIATSSTDDASSRIQMMLALLRTMGRSDAAPLFAARLDAPHFHARWQ
                     AMREFLALDAELALPHLRRMAGS"
     gene            complement(951..1079)
                     /locus_tag="VF547_01740"
     CDS             complement(951..1079)
                     /locus_tag="VF547_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261574.1"
                     /translation="MKIIDIAKLPELRTKVGIHGSARQQEVGGVFCAGVVVVVNSQ"
     gene            complement(1267..1887)
                     /locus_tag="VF547_01745"
     CDS             complement(1267..1887)
                     /locus_tag="VF547_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261575.1"
                     /translation="MTDPDLQRLADLWREPDAADQGAFEALARRARLRGRIVAYLNFA
                     AVAVTVGGVAIGVFMKPGTVTMVAAIALILVTVVVLRKRRQIKQMTRTLETAGREAFI
                     ETSISNATANLRRTRLSLSFFPIGVVVALFYKMSVRVGGRTELMLDAFLEWAPSPRGL
                     VTFAILGLLFAWGLRSASRTKRELRQLEELRSAYAEEAKCDEDEAA"
     gene            complement(1884..2354)
                     /locus_tag="VF547_01750"
     CDS             complement(1884..2354)
                     /locus_tag="VF547_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8261576.1"
                     /translation="MSSSCTGLINRIALSYEADPSLRRELIQDILLAIWVALPSYRGE
                     ASIKTFAASIAQKRCISHVSKRAREPRQVELPNDLVSAAPAPDEAALRNDQKRLLVES
                     IQLLPIPQREAIVLCLEGFSYSEMAIILGISPNAVMLRCQRAKSALKTIVERRS"
     gene            2489..3559
                     /locus_tag="VF547_01755"
     CDS             2489..3559
                     /locus_tag="VF547_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8261577.1"
                     /translation="MERSAQNSGTIEVRTSHRFDSAALEQWLEREVESYRGPLRIEQF
                     RGGQSNPTYKLITPGRSYVLRRKPPGKLLPGAHAVDREYRVITALGRQGFPVARTYGL
                     CLDEAVIGTAFYVMEMVEGRIFWEADFPEVPRADRPAYFDAMNAAMAQLHSIDPEAAG
                     LGDYGKPGNYFERQIGRWSRQYLGDVEAGRVDSMDRLVEWLPRNIPPGEEPARIIHGD
                     FRCDNMIFHPAEPRVLAVLDWELSTLGHPLADFTYHLMVWRMPAGVSTGLAGLDLAAL
                     NIPSEPDYVAAYCRRTGRSSIPDIDFYMAFNMFRLAAIVHGIKGRLARGTASSAHAGE
                     MAASLEPLAERAWQQAVRAGAR"
     gene            3614..>4407
                     /locus_tag="VF547_01760"
     CDS             3614..>4407
                     /locus_tag="VF547_01760"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX8261578.1"
                     /translation="MAQDGADQRLRRALRQIGWARLAGTAVFLVVALVLARYSWQLPM
                     ASDAERALYDMRFERSAQRVVQDDRIVLVTYDDQTLRALQKRSPLDRRVLAEALAALD
                     RMQTRAIGIDILLDQPQAEDAQLLAAFKAMRTPTLIAYATPEQNEDQIGYEQHEFLRG
                     FIAAARTDRVRPASIRMEPDYEDGVVRRWPERKGNLPPQLANAMTRVHPEFGSYSGSI
                     DFLQPAEAERPVFASFAIDTLPLLGEALRDSIRGRYVLIGGNIKDQ"
BASE COUNT          711 a         1499 c         1519 g          678 t
ORIGIN      
        1 gatccggcca tcctcctcag atgcggcaag gccagctcgg catcgagcgc gaggaattcc
       61 cgcatcgcct gccagcgggc gtggaaatgg ggcgcgtcga gccgcgcggc gaagagcggg
      121 gcggcgtcgc tccgccccat cgtgcggagc agggccagca tcatctggat ccgggagctc
      181 gcatcgtcgg tgctgctggt ggcgatcagc tcgaggctgt cggggtcata ttccgtcgcg
      241 accgggctcg aatcgagcga ggtcgaggcg aatatgtaga cgaggtccga ttcggcccgc
      301 tcgacgacga agctctcgcg ccgtccgtcg aaggcgatcg agtcgccgtc ggcgagccgc
      361 ctgcgctcgc gcaggcggca gcgcgcgccc gccttcgcgg tgaagccgga tccgatcatc
      421 ggcgcctccc atatggaaag cacggcattg ccgcccttca ggaagcggaa cagcatgcgc
      481 tggcctgtga aggcgatagg cggcgcgtca acgtcggagc cgcgcttgat cgccagggca
      541 tcggcgccca tcacggccag ctggacgacc aggaggggat ggctgaacag cagcagcccg
      601 tcctgcacct cgttgcgcga cgctcgaagc gggggcctgc acagcggatc ccgcgccgcg
      661 gcggcgagcg aggcgtcgat gaccgcctgg atcgccccgt cgtccccgat gaaggcgccg
      721 gccagcgcca gcaccgcgtc cgagccggct gctgcggcgg cggcgagctc gcgcccgagc
      781 gcggcgaatg ccggtatcga cgcgatccgg gcggagagct ccgccatcgc ctcggcggtg
      841 gccttccgag cgctcgggct ctcgagccac gccgcgagac ggtcgttgat gatcatgctt
      901 tactcctcca aggcgcccgg gggagccgcg ggaccccccg gagccgagat tcactggctg
      961 ttgacgacga cgacgacgcc ggcgcaaaaa acgccgccga cttcctgttg tcgggccgaa
     1021 ccgtggatcc ccaccttggt cctgagctcg ggcagcttcg cgatatcgat gatcttcatc
     1081 tggtcttcct tggttgcagg gttgcgggga cgaagcggca agcgcccgct gcctcggctt
     1141 tcgccgcctg cgacgattgt aaccccgccc gtccccgccc gctcagtcgt gcaggggccg
     1201 cggaatcatt caggggaagc caaattaagc tgtgggtatt tcagggaaaa atgcgtggaa
     1261 aagacgctag gcggcctcgt cctcatcgca tttcgcttcc tcggcatagg cgctgcgcaa
     1321 ttcctccagc tggcggagct cgcgcttggt ccgcgaggcc gatcgaagcc cccaggcgaa
     1381 gagcagcccg aggatcgcga aggtgacgag gccgcggggc gacggcgccc attcgaggaa
     1441 ggcgtcgagc atcagctcgg tccgcccgcc gacccggacc gacatcttgt agaagagcgc
     1501 gacgacgact ccgatgggga agaaggacag gctgagccgg gtccgcctga ggttggccgt
     1561 ggcgttgctg atgctggtct cgatgaaggc ctcgcggccg gcggtctcga gcgtccgggt
     1621 catctgcttg atctgccggc gcttgcgaag gacgaccacg gtgaccagga tgagggcgat
     1681 ggcggccacc atcgtcaccg tgccgggctt catgaagact ccgatcgcca cgccgccgac
     1741 cgtcaccgcg accgcggcga agttcagata ggcgacgatc cggccgcgca gccgggccct
     1801 gcgcgcgagc gcctcgaagg ctccctggtc ggccgcgtcc ggctccctcc acaggtcggc
     1861 caggcgttga agatcgggat cggtcatgag cgtcgctcca cgatcgtctt cagggcgctc
     1921 ttcgcgcgct ggcagcgcag catcacggcg ttcggtgata tgccgagaat gattgccatt
     1981 tcgctatagc tgaagccttc caggcaaagg acgatggctt cgcgctgggg gatgggcagg
     2041 agctggatgg attcgaccag caatcgtttc tggtcgttgc ggagcgccgc ctcgtcgggc
     2101 gccggagcgg cggagacgag gtcgttgggg agctcgacct ggcgcggctc ccgggcgcgc
     2161 ttggagacgt gcgagatgca ccgcttctgg gcgatcgagg cggcgaaggt cttgatcgac
     2221 gcttcgcccc ggtagcttgg cagcgccacc cagatcgcca gcaagatgtc ctggatcagc
     2281 tcgcggcgaa gcgagggatc cgcctcgtac gagagcgcga tgcggttgat caggcccgtg
     2341 cacgacgagc tcatctccgc aaagagttgc tccttagagc cgttcgtggc cattactaca
     2401 ccggtgccca gggccggacc cggccccggc ttatgtgccg catagtagcg cgaagatcaa
     2461 ttcggcggcg ggaaaggaat ttgcgacgat ggagcggagc gcccagaata gcggcacaat
     2521 cgaagtccgg acctcgcaca ggttcgacag cgccgcgctg gagcaatggc tcgagcggga
     2581 agtcgagagc tatcgcggtc cgcttcggat cgagcagttc cgcggcgggc agtccaaccc
     2641 gacctacaag ctgatcaccc ccggccgaag ctacgtcctt cgccgcaagc cgccgggcaa
     2701 gctcctgccc ggcgcccacg ccgtcgaccg cgagtatcgg gtcatcaccg ccctgggccg
     2761 gcagggcttc ccggtggcgc gcacctacgg cctgtgcctc gacgaagcgg tgatcggaac
     2821 cgccttctac gtgatggaga tggtcgaggg ccggatcttc tgggaggccg acttccccga
     2881 ggtgccgcgg gccgaccggc cggcttattt cgacgcgatg aacgcggcga tggcgcagct
     2941 ccattcgatc gatccggaag cggccgggct cggcgactac ggcaagccgg gcaattattt
     3001 cgagcgccag atcgggcgct ggtcccggca atatctcggc gacgtcgagg ccggccgggt
     3061 cgactcgatg gaccggctgg tcgagtggct gccgcgcaac atcccgcccg gagaggagcc
     3121 ggcgcggatc atccacggcg acttccgctg cgacaacatg atcttccacc cggccgagcc
     3181 cagggtgctg gccgtgctcg actgggagct gtcgacgctc ggccatccgc tggccgactt
     3241 cacctatcac ctgatggtct ggcggatgcc cgccggggtc tcgaccggcc tcgccgggct
     3301 cgacctggcg gccctcaaca tcccgtcgga gcccgactat gtcgccgcct attgccggcg
     3361 gaccggccga agctccattc ccgacatcga cttctacatg gccttcaaca tgttccgcct
     3421 cgccgctatc gtccacggca tcaagggccg gctcgcccgg ggcaccgcct cctcggccca
     3481 tgccggcgag atggccgcga gcctcgagcc gctcgccgag cgcgcctggc agcaggcggt
     3541 gcgcgccggc gcccgctagc cccgccgccc ctttgcctct atctgcggag gggcgcggct
     3601 ttccgggggg acgatggcac aggacggtgc ggaccagcgg ctgaggcggg ccttgcggca
     3661 gatcggctgg gcgcggctcg cgggcacggc cgtcttcctg gtcgtggcgc tggtcctcgc
     3721 gcgctattcc tggcagctgc ccatggccag cgacgccgag cgggcgctgt acgacatgcg
     3781 cttcgagcgg tccgcccaga gggtggtcca ggacgaccgg atcgtgctcg tcacctatga
     3841 cgaccagacc ctgcgcgcgc tgcagaagcg ctcgccgctc gaccgaaggg tgctggccga
     3901 ggcgctcgcc gccctcgacc ggatgcagac cagggcgatc ggcatcgaca tcctgctgga
     3961 ccagccgcag gccgaggatg cgcagctcct cgccgcgttc aaggcgatga ggaccccgac
     4021 gctgatcgcc tacgccacgc ccgagcagaa cgaggaccag atcggatatg agcagcacga
     4081 gttcctgcgc ggcttcatcg ccgcggcgcg aaccgaccgg gtccggccgg ccagcatcag
     4141 gatggagccg gactatgagg acggagtcgt ccgccgctgg cccgagcgca aaggcaacct
     4201 cccgccccag ctcgccaatg cgatgacccg ggtccatccc gaattcggaa gctattcggg
     4261 aagcatcgat ttcctccagc cggccgaggc ggagcgcccc gtgttcgcaa gcttcgccat
     4321 cgataccctg ccgctgctcg gcgaggccct gcgcgacagc atccgcggcc gctacgtcct
     4381 catcggcggc aacatcaagg accagga
//
LOCUS       DASVQZ010000064         5117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_783550, whole genome shotgun sequence.
ACCESSION   DASVQZ010000064 DASVQZ010000000
VERSION     DASVQZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783550"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF547_01765"
     CDS             <1..381
                     /locus_tag="VF547_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetylesterase"
                     /protein_id="HEX8261579.1"
                     /translation="HPANKQEVGRRLARAAAAVAYGAREPAGPLAIRARRSGGGGIVV
                     EFAGVTGVLRSWSGSSVVGVELCGATQQSCRYAPAVAEGSRLLIAGDGKPAARVRYAW
                     ADSPVVNLYDEAPLPPGPFELPIE"
     gene            complement(413..2080)
                     /locus_tag="VF547_01770"
     CDS             complement(413..2080)
                     /locus_tag="VF547_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261580.1"
                     /translation="MPATDADLPKPHEAPQSEKLVIAASSLGTVFEWYDFYLYGLLAT
                     IISAQFFSGVNETTGFIFALAAFAAGFAVRPFGAIVFGRIGDIVGRKNTFLVTMGIMG
                     LSTFLVGLLPSYASAGVAAPVLLVLLRLLQGLALGGEYGGAATYVAEHAPNSKRGLYT
                     SFIQTTATLGLFAALLVVIGVRTALGEEAFADWGWRVPFIVSILLLAVSMWIRMQLRE
                     SPVFQKMKDEGRTSRAPLTEAFARWGNLRLVLIALFGAVAGQAVVWYTGQFYALFFLE
                     KMMKVDGATTNMMVAVALAIGTPFFILFGWLSDRIGRRPIILAGCILAALTYFPIFHA
                     LAKAANPALYAAQQAAPVSVVADPADCSVQFDPIGRNRFDARSCDIAKSFLAKAGVSY
                     ENVAAPAGSVAQVRIGGTSIPAVDPGAVSGPARAAAIKAFQAEAAGALEAVGYPDKAD
                     PARIDKPRVILLLTLLVLYVTMVYGPIAALLVEIFPARIRYTSMSLPYHIGNGWFGGF
                     LPTIAFAMVAASGDIYYGLWYPIVVAALTAVIGLVFLRETRGRDIDS"
     gene            complement(2191..3966)
                     /locus_tag="VF547_01775"
     CDS             complement(2191..3966)
                     /locus_tag="VF547_01775"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX8261581.1"
                     /translation="MRTPVRPFRSRDWFAAPGRSDMAALYLERFMNYGLTPEELRCGK
                     PIVGIAQSGSDLAPCNRIHLELAHRVRAGIRDAGGIPMEFPTHPIFENCRRPTAALDR
                     NLSYLGLVEILNGYPIDAVVITTGCDKTTPAGIMAASTVDIPAITLSGGPMLDGWHEG
                     ELVGSGTVIWRSRRLLGAGEIDEEEFLRRATDSAPSAGHCNTMGTASTMNAVSEALGM
                     SLPGNAAIPAPYRERGQMAYETGRRAVELAYEDLRPSRILTRNAFLNAIRVNAAIGGS
                     SNAQPHIVAMARHAGVEIGPEDWMAHGYDVPLLVNMQPAGKYLSERFHRAGGVPAVMW
                     ELLQAGRLDGAAITVTGRTVEANLEGREATDREVILPFARPLRERAGFLVLKGNLFDF
                     AIMKTSVISEEFRRRYLSRPGMEGVLEARAAVFDGSDDYHHRINDPELDIGEDSILVI
                     RGAGPLGWPGSAEVVNMQPPDRLLQRGIASLPTLGDGRQSGTSDSPSILNASPESAAG
                     GGLAWLRTGDVIRIDLNSGTCDALVDEAEIARRKAQAPPATPASNTPWEELYREKTGQ
                     LAEGGVLEFAVKYRGTSAKTPRHNH"
     gene            complement(4109..>5117)
                     /locus_tag="VF547_01780"
     CDS             complement(4109..>5117)
                     /locus_tag="VF547_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX8261582.1"
                     /translation="AGIRGRMTTIQQIMIITGLTGAFLVNYFLAASAGESTRAFWGGL
                     EAWRWMYLMQAVPAAIFLVALFFIPESPRYLVSKGRDEQARNVLTSLFGGEVASAKLE
                     EIRASFSQDHRPRLSDLKRPDGRGIRPIVWAGILLAVFQQLVGINVIFYYGATLWQLA
                     GFTEDQSLQINIVSGAVSIAACFITIAVIDKIGRKPLLLIGSAGMAATLFAMVYAFAN
                     GTLDAAGNLQLSSGLGTVALIAANLYVIFFNVSWGPVMWVMLGEMFPNQIRGSALAVA
                     GFAQWFSNYLIAQSFPIMAASLGLAVSYSFYAVSAVISFFLVQKFIHETKGKELEEMP
                     G"
BASE COUNT          812 a         1647 c         1903 g          755 t
ORIGIN      
        1 catccggcca acaagcagga ggtgggccgg cggctggccc gcgccgccgc ggccgtcgcc
       61 tacggggcgc gggagccggc ggggccgctg gcgatccggg cgcgtcgaag cggcggcggc
      121 ggaatcgtcg tcgagttcgc gggcgtcacc ggcgttctgc gcagctggag cggctcgagc
      181 gtcgtcggcg tcgagctgtg cggcgccacc cagcaaagct gccgctatgc gccggccgtc
      241 gccgagggga gcaggctgct gatcgccggc gacgggaagc ccgccgcgcg ggtccgctat
      301 gcctgggccg acagccccgt cgtcaacctc tatgacgagg cgccgctgcc cccggggccg
      361 ttcgagcttc cgatcgaatg accctgccgc ccctttccgg cggcgtcggg gctcaggagt
      421 cgatgtcccg gccgcgggtt tcgcgaagga agaccaggcc gatgaccgcg gtcagcgccg
      481 cgaccacgat cgggtaccat aggccgtaat agatgtcgcc gctggcggcg accatggcga
      541 aggcgatggt cggcaggaag cctccgaacc agccgttgcc gatatggtag ggcagcgaca
      601 tcgaggtgta gcggatgcgc gcggggaaga tctcgacgag cagggccgcg atcggcccgt
      661 agaccatggt cacgtagagg acgaggaggg tgaggaggag gatcacgcgc ggcttgtcga
      721 tccgggcggg gtcggccttg tcggggtagc cgaccgcctc gagcgcgccg gccgcctcgg
      781 cctggaaggc cttgatcgcc gcggccctcg ccgggccgga gacggcgccg ggatcgacgg
      841 ccgggatgga cgtgccgccg atcctcacct gggccaccga cccggccggg gcggcgacgt
      901 tttcatagct gacccccgcc ttggcgagga acgacttggc gatgtcgcag gagcgggcgt
      961 cgaacctgtt gcgcccgatc gggtcgaact ggaccgagca gtcggcgggg tccgcgacga
     1021 cgctcaccgg cgccgcctgc tgcgccgcgt agagggcggg attggcggct ttggcgagcg
     1081 cgtggaagat cgggaaatag gtgagggcgg cgaggatgca gccggcgagg atgatcggtc
     1141 gccggccgat gcggtcggac agccagccga acaggatgaa gaagggcgtg ccgatcgcca
     1201 gcgccaccgc gaccatcatg ttggtggtgg cgccgtccac cttcatcatc ttctcgagga
     1261 agaacagcgc gtagaactgg cccgtgtacc agaccaccgc ctggccggcg accgcgccga
     1321 acagggcgat cagaaccagc ctgagattgc cccagcgcgc gaacgcctcg gtcagcggcg
     1381 ccctggaggt ccggccctcg tccttcatct tctggaagac ggggctctcg cgaagctgca
     1441 tccggatcca catcgacacg gcgagaagca ggatcgagac gatgaagggc acccgccagc
     1501 cccagtcggc gaaggcctcc tcgccgagcg cggtgcggac gccgatcacg accagcaagg
     1561 cggcgaacag gccgagggtg gcggtcgtct ggatgaagct ggtgtagagg ccgcgcttcg
     1621 aattgggcgc gtgctcggcg acgtaggtcg cggcgccgcc atattcgccg ccgagcgcga
     1681 ggccctggag gaggcgcagc aggacgagca ggaccggcgc ggccactccg gcgctggcgt
     1741 agctgggcag caggccgacg aggaaggtcg agaggcccat gatgcccatg gtgacgagga
     1801 aggtgttctt gcggccgacg atgtcgccga tgcggccgaa gacgatcgct ccgaacggcc
     1861 gaacggcgaa gcccgcggcg aaggcggcga gggcgaagat gaagccggtc gtctcgttga
     1921 cgccggagaa gaactgggcc gagatgatcg tcgccagcag gccgtagaga tagaagtcgt
     1981 accattcgaa gaccgtcccg agcgaggagg cggcgatgac cagcttctcg ctctggggcg
     2041 cctcgtgcgg cttgggcagg tctgcgtcgg tggccggcaa ggacagtctc ccttagctcg
     2101 caccgtcatt gcggggagcc tggcggcgcg gcaacccggc gtgccttccg aggcggctct
     2161 ggattgcctc gcctcgctcg cgatgacgag tcaatggttg tgcctgggcg tcttcgccga
     2221 cgtgccgcgg tatttcaccg cgaactccag cacgccccct tcggcgagct ggccggtctt
     2281 ctcccgatac agctcttccc aaggggtgtt gctcgccggc gtcgccggcg gcgcctgcgc
     2341 cttccgcctc gcgatctcgg cctcgtcgac cagcgcgtcg caggtgcccg agttcaggtc
     2401 gatgcggatg acgtcgccgg tgcggagcca ggcgaggccg ccgccggccg cgctctccgg
     2461 cgaggcgttg aggatcgacg ggctgtcgga cgtgcccgac tgccggccgt cgccgagcgt
     2521 cggaaggctg gcgatgccgc gctggaggag ccggtccggg ggctgcatgt tgaccacctc
     2581 ggccgagccc ggccagccca gaggaccggc gccgcggatg acgaggatcg aatcctcgcc
     2641 gatgtcgagc tcgggatcgt tgatccgatg gtgatagtcg tccgagccgt cgaagaccgc
     2701 ggcgcgcgcc tcgagcacgc cctccatgcc cggccgcgac aggtagcggc ggcggaactc
     2761 ctcggagatg acgctcgtct tcatgatcgc gaagtcgaac agattgccct tgaggacgag
     2821 gaacccggcg cgttccctga gcggccgggc gaaggggagg atgacctccc ggtcggtcgc
     2881 ttcccggccc tccagattcg cctcgacggt gcggccggtg acggtgatcg cggcgccgtc
     2941 gagccggccg gcctgaagca gttcccacat cacggcgggc actccgccgg cgcggtggaa
     3001 gcgttcgctg agatattttc cggccggctg catgttgacc agcaagggca cgtcatagcc
     3061 gtgcgccatc cagtcctcgg ggccgatctc cacgccggcg tgacgggcca tggcgacgat
     3121 gtgcggctgg gcgttgctcg agccgccgat cgcggcgttg acccggatcg cgttgaggaa
     3181 ggcgttgcgg gtgaggatcc gcgacggccg caggtcctca taggcgagct cgaccgcgcg
     3241 gcgtccggtc tcgtaggcca tctggccgcg ctcccgatag ggggcgggga tggcggcatt
     3301 gccgggaagc gacattccga gcgcctcgga gacggcgttc atcgtcgagg ccgtgcccat
     3361 cgtgttgcaa tggccggccg agggggccga atcggtcgct cggcggagga actcctcctc
     3421 gtctatctcc cccgctccga gcagccggcg cgagcgccag atcaccgttc ccgagccgac
     3481 cagctcgcct tcgtgccagc cgtcgagcat cggcccgccg gagagggtga tggccggaat
     3541 gtcgaccgtc gaggcggcca tgatcccggc cggggtggtc ttgtcgcagc cggtggtgat
     3601 gacgaccgcg tcgatcggat agccgttcag tatctcgacc aggccgagat aggagaggtt
     3661 gcggtcgagc gccgcggtcg gccgccggca gttctcgaag atcgggtggg tcgggaactc
     3721 catcgggatc ccgcccgcgt cgcggattcc ggcgcggacc cggtgggcga gctcgaggtg
     3781 gatccggttg cacggagcga ggtcggagcc ggactgggcg atgccgacga tcggcttgcc
     3841 gcagcgcaat tcctccggcg tcaggccgta gttcatgaac cgctcgaggt agagcgccgc
     3901 catgtcgctc cggcccggcg cggcgaacca gtcgcgcgag cggaacgggc ggacgggagt
     3961 cctcacctgt cgtccctgcc ggtgcagcgg gcggggaggg ggaccagccg aagggtggtg
     4021 gaggggtaat ggcggaggct tcggcctgct gcccctccac caggcttcgc ctgttccccc
     4081 tcgccatctg cgatggggag gatcgcgatc atcccggcat ctcctcgagc tccttgccct
     4141 tcgtctcgtg gatgaacttc tggacgagga agaagctgat caccgcggag acggcgtaga
     4201 agctgtagct gaccgccagg cccagggagg cggccatgat cgggaagctc tgggcgatca
     4261 gatagttgga gaaccattgc gcgaagccgg ccacggcgag ggcggagccg cggatctggt
     4321 tggggaacat ctcgcccagc atcacccaca tcaccgggcc ccagctgacg ttgaagaaga
     4381 tcacgtagag gttggcggcg atcagcgcga ccgttccaag cccgctcgag agctgcaggt
     4441 tgccggcggc gtcgagcgtg ccgttggcga aggcgtagac catcgcgaac agggtcgccg
     4501 ccatgccggc cgagccgatc aggagcagcg gcttgcggcc gatcttgtcg atgacggcga
     4561 tggtgatgaa gcaggccgcg atcgacactg cgcccgagac gatgttgatc tggagggact
     4621 ggtcctcggt gaagccagcg agctgccaca aggtggcgcc gtaatagaag atgacgttga
     4681 tcccgacgag ctgctggaac accgccagca ggattcccgc ccagacgatc ggccggatgc
     4741 cgcggccgtc cggacgcttg aggtcgctca gcctgggccg gtggtcctgg ctgaagctgg
     4801 cccggatctc ctcgagcttg gcgctcgcga cctcgcctcc gaacagcgag gtcagcacgt
     4861 tgcgcgcctg ctcgtcgcga cccttggaga ccaggtagcg cgggctttcg gggatgaaga
     4921 agagcgcgac gaggaagatg gcggcgggga ccgcctgcat cagatacatc cagcgccagg
     4981 cctccagccc gccccagaag gcccgggtcg attcgcccgc ggaggccgca aggaagtagt
     5041 tgaccaggaa cgccccggtg aggccggtga tgatcatgat ctgctggatc gtcgtcatcc
     5101 ggccgcggat gccggcg
//
LOCUS       DASVQZ010000065         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_590944, whole genome shotgun sequence.
ACCESSION   DASVQZ010000065 DASVQZ010000000
VERSION     DASVQZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590944"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VF547_01785"
     CDS             <1..957
                     /locus_tag="VF547_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX8261583.1"
                     /translation="FIEGTYPHPLPCIPGHEAAGVVEAVGSEVRTVKAGDHVVTCLSA
                     YCGHCEYCVTGRMSLCPGGDTRRARGAPPRITAADGSAINQMLNLSGFAEAMLVHEHA
                     CVAIDPEMPLDRAAVIGCAVTTGAGTVFNACKLVPGETVAVIGCGGVGLATVNAARIA
                     GAGRIIAADPVPEKRELAIRLGATDVVDPTDETAGAQIVEMTKGGVDHAIEAVGRPAS
                     AALAVKSLRRGGTATILGMMPLDQKVGLGAMDLLSGKKLQGAFMGANRFPVDIPRLVD
                     FYLRGLLDLDSIVAETIPLERVNEGFQRMKRGDAARSVVVFD"
     gene            1037..1810
                     /locus_tag="VF547_01790"
     CDS             1037..1810
                     /locus_tag="VF547_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8261584.1"
                     /translation="MSLFDLSGKVAVITGSSRGIGRAIAEAMADQGAKVVISSRKAEA
                     CQAVADSINAEHEGAAIVVPASISSKEALQTLVDETRRRLGRIDVLVCNAASNPYYGP
                     MGGISDDQFRKILENNVIANHWLIQMVAPEMIERKAGSIIIVSSIGGLKASAVIGAYN
                     VSKAADFQLARNLAAEFGPHQVRVNCIAPGLIRTDFARALWENPDTLKMVTAHTPLQR
                     IGEPHEIAGAAVFLASDASTFVTGQAIVVDGGSTVGVGL"
     gene            1905..>2238
                     /locus_tag="VF547_01795"
     CDS             1905..>2238
                     /locus_tag="VF547_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8261585.1"
                     /translation="MRLADKIAIVTGAGSGIGRASAIRFAAEGARVICADLKGQDETA
                     AAIGAAALAVQADAGSEDDVQDLVQAALQHFGGLDVFFANAGVSGGLASVFEQGVEDW
                     QEVLRVNLI"
BASE COUNT          353 a          803 c          752 g          330 t
ORIGIN      
        1 ttcatcgagg ggacctatcc ccacccgctg ccctgcatcc ccggccacga agccgccggc
       61 gtggtcgagg cggtggggag cgaggtccgg acggtcaagg cgggcgacca tgtcgtcacc
      121 tgcctctccg cttattgcgg ccattgcgaa tattgcgtga ccgggcggat gtcgctctgc
      181 cccggcggcg acacccgccg ggcccgcggc gctccgcccc ggatcacggc ggcggacgga
      241 agcgcgataa accagatgct caacctctcc ggcttcgccg aggcgatgct ggtccacgag
      301 catgcctgcg tcgccatcga cccggaaatg ccgctcgacc gggccgcggt gatcggctgc
      361 gcggtgacca ccggcgccgg taccgtcttc aacgcctgca agctggttcc cggcgagacg
      421 gtggcggtga tcggctgcgg cggggtcggc ctcgccacgg tcaacgccgc gcggatcgcc
      481 ggcgccggcc ggatcatcgc cgccgatccg gtgcccgaga agcgcgagct cgcgatcagg
      541 ctcggagcga ccgacgtcgt cgacccgacg gacgaaacgg ccggggcgca gatcgtcgag
      601 atgaccaagg gcggggtcga ccatgccatc gaggcggtcg gccgccccgc ctccgccgcg
      661 ctcgcggtca agtccctccg ccgcggcggc accgccacca tcctcggcat gatgccgctg
      721 gaccaaaagg tcgggctcgg agcgatggac ctgctctcgg gcaagaagct ccagggcgcc
      781 ttcatgggcg ccaaccgctt cccggtcgac atcccccgcc tcgtcgattt ctacctgcgc
      841 gggctgctcg atttggactc gatcgtcgcc gagacgatcc cgctggaacg ggtcaacgaa
      901 ggcttccagc ggatgaagcg cggcgacgcg gcgagatcgg tcgtggtgtt cgattagctc
      961 cgtcacccca gcgaaggctg gggtctatac ggagtcggca tggaccccag ccttcgctgg
     1021 ggtgacggag gatggaatga gcttgttcga cctcagcggc aaagtcgccg tgatcaccgg
     1081 ctcgtcgcgc ggcatcggcc gggcgatcgc cgaggcgatg gcggaccagg gcgcgaaggt
     1141 ggtcatctcc tcgcgcaagg cggaggcctg ccaggcggtc gccgactcga tcaacgccga
     1201 gcatgagggc gccgccatcg tcgtccccgc ctccatctcg tcgaaggaag ccctccagac
     1261 cctcgtcgac gagacccgcc gccggctcgg ccggatcgac gtcctcgtct gcaacgccgc
     1321 ctccaacccc tattacgggc cgatgggcgg gatcagcgac gaccagttcc gcaagatcct
     1381 cgagaacaac gtcatcgcca accactggct gatccagatg gtcgcccccg agatgatcga
     1441 gcgcaaggcc ggctcgatca tcatcgtcag ctcgatcggc gggctcaagg cctcggccgt
     1501 gatcggcgcc tacaacgtct ccaaggccgc cgacttccag ctcgcccgca acctcgccgc
     1561 cgagttcggc ccccaccagg tccgggtcaa ttgcatcgcc ccgggcctga tccgcaccga
     1621 tttcgcccgg gcgctgtggg agaatcccga cacgctcaag atggtcaccg cccacacccc
     1681 gctccagcgc atcggcgagc cccacgagat tgccggcgcg gccgtcttcc tggcgagcga
     1741 cgcctccacc ttcgtgacag gccaggcgat cgtggtagat gggggaagca ccgttggagt
     1801 tgggctctag gccgtcattg cgagcgaagc gaagcaatcc agtgctgcgc cgctgcccgc
     1861 tggatggccg cgtcgccagg ctcctcgcaa tgacgaggta aaccatgaga ctcgccgaca
     1921 agatcgccat cgtcaccggc gccggctcgg ggatcggccg ggcgagcgcg atccgcttcg
     1981 ccgccgaggg cgcgcgcgtg atctgcgcgg acctcaaggg ccaggacgag accgcggccg
     2041 cgatcggcgc cgccgcgctc gcagtccagg ccgacgcggg cagcgaggac gacgtccagg
     2101 acctcgtcca ggccgccctc cagcatttcg gcggcctcga cgtcttcttc gccaacgccg
     2161 gcgtgtcggg cggcctcgcc tcggtcttcg agcagggcgt cgaggactgg caggaggtgc
     2221 ttcgggtcaa tctgatcg
//
LOCUS       DASVQZ010000066         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_493999, whole genome shotgun sequence.
ACCESSION   DASVQZ010000066 DASVQZ010000000
VERSION     DASVQZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493999"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1190)
                     /locus_tag="VF547_01800"
     CDS             complement(81..1190)
                     /locus_tag="VF547_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX8261586.1"
                     /translation="MDKPLAGLKVVELARILAGPWAGQLLADLGAEVVKIERPEIGDD
                     TRHWGPPFAADGAAAYFHACNRGKRSLAIDLDTAQGQAQVRALAAEADVLIENFKVGG
                     LAKYGLDYASLAVPNPGLVYCSITGFGQDGPYASRAGYDFVIQAMGGAMSLTGEPDGP
                     PQKSGVAYADMFTGLYATVAILAALRERDRTGRGAHVDMALLDTQVAVLGNQALYWMV
                     SGEVPRRMGNGHVNLVPYQAFEVADGEIVVAVGNDRQFERLCAILGLESLARDERFAT
                     NPARVVNRAALIPILERAVRTRRRAELSDALEAAGVPAGPINAVDAVFADPQVVHRGL
                     RIEGASPGLASPIVIDGLRQVSERPAPGLGEAGPC"
     gene            complement(1272..>1525)
                     /locus_tag="VF547_01805"
     CDS             complement(1272..>1525)
                     /locus_tag="VF547_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8261587.1"
                     /translation="RVGRRLDEGELIPETISLIKRNNVGKALDIARTARDMHGGNGIS
                     AEFQVMRHAMNLETVNTYEGTHDVHGLILGRAITGISAF"
BASE COUNT          241 a          524 c          519 g          241 t
ORIGIN      
        1 cctcgtatcg cgcgatgtga tcagaagcac ctcgatgccg ccacttgccg agcggcggac
       61 ggggatgact ccatattgga tcagcacggc cccgcctccc cgagccccgg cgccggacgc
      121 tccgagacct ggcggaggcc gtcgatgacg atcggcgagg cgaggccggg gctcgccccc
      181 tcgatacgca ggccgcgatg gacgacctgc gggtcggcga acaccgcgtc gacggcgttg
      241 atcggcccgg cggggacgcc cgcggcctcc agcgcgtcgg acaattccgc ccgccggcga
      301 gtgcgcactg cccgctcaag aatcgggatc agcgccgccc ggttgacgac tcgcgccgga
      361 ttggtggcga agcgctcgtc gcgggcgagc gattccagcc ccaatatcgc gcagagtcgc
      421 tcgaactggc ggtcgttgcc gaccgcgacg acgatctcgc cgtcggcgac ctcgaaagcc
      481 tggtagggga cgaggttgac gtggccgttg cccatccgcc gcggcacctc gccggaaacc
      541 atccagtaga gcgcctggtt gccgagcacc gcgacctggg tgtcgagcag ggccatgtcg
      601 acatgggcgc cgcggccggt gcggtcccgc tccctcagcg cggcgaggat ggccaccgtc
      661 gcatagaggc ctgtgaacat gtccgcatag gcgacccccg atttctgcgg agggccgtcg
      721 ggctcgccgg tcagggacat cgccccgccc atcgcctgga tgacgaagtc gtagcccgcc
      781 cgcgaggcgt agggtccgtc ctgtccgaag ccggtgatcg agcaatagac gaggccgggg
      841 ttcggcacgg cgagggaagc gtagtcgagc ccgtatttcg ccagcccgcc caccttgaaa
      901 ttctctatca gcacgtcggc ttcggccgcc agggcccgca cctgggcctg gccttgggcg
      961 gtgtcgagat cgatcgccag cgaccttttg ccgcggttgc aggcgtggaa ataagccgcc
     1021 gctccgtcgg cagcgaaagg cggcccccaa tgccttgtat cgtcaccgat ctccggccgc
     1081 tcgatcttca cgacctcggc gccgagatcc gccagcagct gtcccgccca cggaccggca
     1141 aggatgcgcg caagctccac caccttcagg ccagcaagag gcttatccat gctcctccgt
     1201 cgccccggcg aaagctgggg tccatgctga gggggtacgg attccagctt tcgctggaat
     1261 gacggggaag gtcaaaacgc cgagatcccc gtgatcgccc gtcccaggat caggccgtgg
     1321 acgtcgtgcg tgccctcgta ggtgttgacc gtctcgaggt tcatcgcgtg gcgcatcacc
     1381 tggaactcgg ccgagatgcc gttgccgcca tgcatgtcgc gggcggtacg ggcgatgtcg
     1441 agcgccttgc cgacattgtt gcgcttgatg aggctgatcg tctcggggat gagctcgccc
     1501 tcgtcgaggc ggcggccgac ccgaa
//
LOCUS       DASVQZ010000067         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_591494, whole genome shotgun sequence.
ACCESSION   DASVQZ010000067 DASVQZ010000000
VERSION     DASVQZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591494"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..2770)
                     /locus_tag="VF547_01810"
     CDS             complement(2..2770)
                     /locus_tag="VF547_01810"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX8261588.1"
                     /translation="MATRFASVPNKRAIIDRRALADALSEAPDGAAAILKRALNEGRA
                     EIARRLEDKPYAGSESAAAYAFLTDQIVRLAYERAVASLPPRGPGDRLLLLAVGGYGR
                     GEMALHSDIDIAFVTPALPGAWAKQAVEAILYLLWDLGLKLGPSTRSTDELIDLAGSD
                     HTVRTALLEARWLWGDEALFEEAQIRFWKEVVAGKAKSFVTEKLEERNARHRRMGDSR
                     YVVEPNVKEGKGGLRDLHTLFWIGKYAYQVRSVADLVGVGLLSQSELHQFQRAERFLW
                     AVRCQLHLVAGRAEERLTFDYQREIATRMRYADRPGKSPVERFMRHYFLHAKMVGDLT
                     GVFLAHLDEKFARKGGRLGLPAIRRRPSRLEGFVLDRGRLALPHDDYFREDPVRLLQI
                     FALADLYGLEIHPLAMRAAARDSKLVDQEVRSDPRANALFLDVLTSPRDPETVLRWMN
                     EAGVFGRFVPDFGRVVAQMQFDMYHHYTVDEHSIRAIGLLSRIQKGESKEDHPLATAL
                     FRHIASRRVLYAAVLLHDIAKGRGGDHSVLGAEVARSLCPRLGLSEAETETVAWLVRW
                     HLLMSATAFKRDLADPQTIQDFAARVQSPERLRLLFILTVVDIQAVGPGVWNGWKRQL
                     LRELFDAAEEALRLGHKQKGRSERIAATQAELAARLGWDDSRFARHAWRLPDSYWLAE
                     PIDVLEKNAALIDSADRAPEKTGPLVASIEEERGATLLSVYAKDRPGLFYRLSAAISL
                     AGANIIDARIHTTLDGMAVDNFLVQDASGGPFADRHQLDRLQENVLRAVEGQEPAMDR
                     LEAKALPLLRAEAFAIQPEVFVDNRASSRYTVVEVNARDRAALLGGLARALYESKAAV
                     HSAHIATYGERAVDVFYLTDLNGSKIEAPARLKALRANLLEAAAENDAGHRLARPAPP
                     GNRAISRP"
     gene            complement(2770..>3348)
                     /locus_tag="VF547_01815"
     CDS             complement(2770..>3348)
                     /locus_tag="VF547_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX8261589.1"
                     /translation="MVEMVETAAILAQATPRSLVILDEIGRGTSTYDGLAIAWAVTEA
                     IHDRNRCRCLFATHYHELTRLAERLDSLSLHHVRAREWKGDLVLLHEVAKGPADRSYG
                     LAVAKLAGLPGPVLARARSVLAKLEQGRAATGGIAAGLDDLPLFAAAAAQEEPAPEPV
                     QAALAEVDADSLSPREALELVYRLKRLAAGEG"
BASE COUNT          499 a         1151 c         1182 g          516 t
ORIGIN      
        1 cctaaggtct tgagattgcc cggttgccgg ggggcgccgg ccgggcgagc cggtgcccgg
       61 catcgttttc cgcggcggcc tcaagcaggt tcgcccggag cgccttcagc cttgcggggg
      121 cctcgatctt cgagccgttg agatccgtca ggtagaagac gtcgacggcc cgctcgccat
      181 aggtggcgat atgggcggaa tggaccgcgg ctttcgattc gtacagcgcc cgggcgaggc
      241 cgccgagcag ggcggcccgg tcgcgcgcat tgacctcgac cacggtgtag cggctcgacg
      301 cccgattatc gacgaacacc tcgggctgga tggcgaaagc ctccgctcgg agcagcggaa
      361 gggccttggc ctccagccgg tccatggccg gctcctgccc ctcgaccgct cgcagcacat
      421 tctcctgcag ccggtcgagc tggtggcggt ccgcgaacgg tccgcccgag gcgtcctgga
      481 ccaggaaatt gtcgaccgcc attccgtcca gggtggtgtg gatccgggcg tcgatgatgt
      541 tggctccggc gaggctgatc gcggcgctga gccggtagaa gaggcccggc cggtccttgg
      601 cgtagacgct gagcagggtc gctccgcgct cctcctcgat cgaagcgacg agcggaccgg
      661 tcttctccgg cgcccggtcg gcggagtcga tcaacgccgc attcttctcg agaacgtcga
      721 tcggctccgc gagccagtag ctgtcgggca gtcgccaggc gtgccgggcg aaccgggaat
      781 cgtcccagcc cagcctggcg gcgagctccg cctgggtcgc ggcgatccgc tcgctccggc
      841 ccttctgctt gtggcccagc cgaagcgcct cctcggcggc gtcgaacagc tcgcgaagca
      901 gctgccgctt ccagccgttc cacacgcccg ggccgacggc ctgtatgtcc acgacggtga
      961 ggatgaagag gagtcgaagc cgctccgggc tctgcacccg ggcggcgaag tcctggatgg
     1021 tctgcgggtc cgcgaggtcg cgcttgaagg cggtcgcgga catgagcagg tgccagcgaa
     1081 cgagccaggc gacggtctca gtctccgcct cgctcaggcc cagcctcggg cagagcgacc
     1141 gggccacctc cgcgccaagg acgctatggt cgccgccgcg ccccttggcg atgtcgtgga
     1201 gaaggacggc cgcatagagg acgcggcgcg acgcgatgtg gcggaacagc gccgtggcga
     1261 gcggatggtc ctccttcgac tcgcccttct ggatccggga gaggaggccg atcgcccgga
     1321 tcgaatgctc gtcgaccgta taatggtggt acatgtcgaa ctgcatctgg gcgacgaccc
     1381 tgccgaaatc gggaacgaag cggccgaaca cccccgcctc gttcatccac cgcagcaccg
     1441 tctccgggtc gcgggggctg gtcagcacgt cgaggaacag cgcgttggcg cgaggatcgg
     1501 agcgcacctc ctggtcgacc agcttcgaat cgcgggctgc ggcgcgcatc gccagcgggt
     1561 ggatctcgag gccgtagagg tcggccaaag cgaagatctg gagcagccgg accggatcct
     1621 ctcgaaaata atcgtcgtgg ggcaaagcga gccggccgcg gtcgagcacg aacccctcca
     1681 gccggcttgg ccggcggcgg atcgccggca gaccaagccg gccgcccttt ctcgcgaact
     1741 tctcgtcgag atgggcgagg aagaccccgg tcaggtcccc gaccatcttc gcgtggagga
     1801 aatagtggcg catgaagcgc tcgaccggcg acttgcccgg ccggtcggcg tagcgcatcc
     1861 gggtggcgat ctcgcgctga tagtcgaagg tgaggcgctc ctcggcccga ccggccacga
     1921 ggtggagctg gcaccgcacc gcccacagga atcgctcggc gcgctggaac tggtgcagtt
     1981 ccgactggga gaggagcccc actccgacca ggtccgccac gcttctgacc tgataggcat
     2041 atttgccgat ccagaagagc gtatggaggt cgcgcagccc gcccttcccc tccttgacgt
     2101 tgggttcgac gacgtagcgg ctgtcgccca tccgccggtg tcgggcgttc ctttcctcca
     2161 gcttctcggt gacgaagctt ttcgccttgc cggccaccac ctccttccag aagcggatct
     2221 gggcctcttc gaacagcgcc tcgtcccccc acagccagcg cgcctccagg agcgccgtcc
     2281 ttaccgtatg gtcggatccg gcgaggtcga tcagctcgtc ggtggagcgg gtggaagggc
     2341 cgagcttcag ccccaggtcc cagaggaggt agaggatcgc ctccaccgcc tgcttcgccc
     2401 aagcccccgg cagagcgggc gtgacgaagg cgatgtcgat gtcggaatga agcgccatct
     2461 ccccccgtcc gtagccgccg acggccagaa gcagcagccg gtcgcccggt ccccgcggcg
     2521 ggagcgacgc gaccgcccgc tcgtaggcga gccgcacgat ctggtcggtg aggaaggcat
     2581 aggcggcggc gctctcgctc cctgcatagg gcttgtcctc gagccggcgg gcgatttcgg
     2641 cccggccctc gttgagggcg cgcttcagga tcgccgccgc cccgtccggg gcctcggaga
     2701 gcgcgtcggc gagcgcccgc cggtcgatga tcgccctctt gttcgggacg gaggcgaagc
     2761 gggtcgccat cagccctcgc cggcggccag ccgcttcagc cggtagacga gctccagggc
     2821 ctcgcgcggg ctgagcgagt cggcgtccac ctcggcgagt gccgcctgca ccggctcagg
     2881 ggccggctcc tcctgcgccg ccgccgccgc gaacaggggc aggtcgtcga gcccggcggc
     2941 gatcccgccg gtggcggctc ggccctgctc gagcttggcc agcacggatc tcgcccgggc
     3001 aagcaccggc cccggcaggc ccgcgagctt ggcgaccgca aggccgtagc tccggtcggc
     3061 cgggcccttg gcgacttcgt gcagcaggac gaggtcgccc ttccactcgc gcgcgcggac
     3121 gtggtggagg gacagggaat cgagccgctc ggcgagtcgg gtgagctcgt gataatgggt
     3181 tgcgaacagg cagcggcagc ggttgcggtc gtggatcgcc tccgtcaccg cccaggcgat
     3241 ggcgaggccg tcataggtcg aggtcccccg cccgatttcg tcgaggatga cgagacttcg
     3301 gggtgtcgcc tgcgcaagaa tcgcggcggt ctctaccatc tcgaccat
//
LOCUS       DASVQZ010000068        12607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_494343, whole genome shotgun sequence.
ACCESSION   DASVQZ010000068 DASVQZ010000000
VERSION     DASVQZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12607
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494343"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /gene="plsY"
                     /locus_tag="VF547_01820"
     CDS             complement(<1..603)
                     /gene="plsY"
                     /locus_tag="VF547_01820"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX8261590.1"
                     /translation="MQAIVAPLAALLLGYALGSIPFGILLTRLGGAGDLRQVGSGNIG
                     ATNVLRTGRKGLAAATLLLDMAKGAAALVLADRLLPGTVQLAAIGAMVGHLWPVWLRF
                     AGGKGVATYFGVVLGFALVDGLGWPAPAAYAAAWLGVLLATRYSSLGGLAAAAAMPVA
                     AGLLGRFDLALLFLGFTLLVFWSHRSNIGRLLAGTEPRVGG"
     gene            850..1647
                     /gene="murI"
                     /locus_tag="VF547_01825"
     CDS             850..1647
                     /gene="murI"
                     /locus_tag="VF547_01825"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX8261591.1"
                     /translation="MTSGRPLLVFDSGVGGLSVLGAIRGLLPTAPVVYAADSAGYPYG
                     TRRPAEIEARVPALLGRLAERHDPELIVIACNTASTIALDSVRAALDLPVVGTVPAIK
                     PAALLSTTRTIGVLGTEATVRQPYVDRLSAEFAADCTVIRHGSAELVDLAEAKLRGEP
                     PDPDSVSAALAGLFARPGGGRIDTVVLACTHFPLIEAELAAAAPHPVRFVDGKEGIAR
                     RTAWLTRGRRWPEAPSEGIALFTGRGTDVEAYRPGLARYGLTRIERL"
     gene            1752..2966
                     /gene="hemA"
                     /locus_tag="VF547_01830"
     CDS             1752..2966
                     /gene="hemA"
                     /locus_tag="VF547_01830"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX8261592.1"
                     /translation="MDYNRIFAQAIDRLHAEGRYRVFIDILRNRGTFPNARCFAGHNG
                     PKPITVWCSNDYLCMGQHPAVVEAMEEALHDVGAGSGGTRNIGGNTHYHVELEAELAD
                     LHGKEAALLFTSGYISNEATLSTVAKLLPGCIIFSDELNHASMIAGIRASGCEKRVFR
                     HNDLEHLEALLAEADPESPKLIAFESVYSMDGDVAPIGAFCDLADRYNAITYLDEVHA
                     VGMYGPRGGGISERDDVARRVTIIEGTLGKAFGVMGGYIAADRNIVDVVRSYAPGFIF
                     TTSLSPVLVAGALASVRHLKASCAEREAQQANAERLKAIFEDARLPVMPSTTHIVPLM
                     VGDPVKAKKISDILLAEYGCYVQPINYPTVPRGTERLRFTPGPRHDEAMMRELAKALV
                     EIWDRLELRAAA"
     gene            3160..3618
                     /gene="rpiB"
                     /locus_tag="VF547_01835"
     CDS             3160..3618
                     /gene="rpiB"
                     /locus_tag="VF547_01835"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX8261593.1"
                     /translation="MAERIAIVSDHAAFEMKAALAAYLRDGGHAVTDLGPDGIDSVDY
                     PDYGYKLADEIAAGRADLGVALCGSGIGISIAANRHPACRCALVSEPLSARLAREHND
                     ANAIAMGGRLLGLEMAKACLDAFLATSFGGGRHQRRVDKLSNPAFARENA"
     gene            3615..4952
                     /locus_tag="VF547_01840"
     CDS             3615..4952
                     /locus_tag="VF547_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX8261594.1"
                     /translation="MSSRSAFDFAEVQHSGFFTGRLAEADPAVAEAVAAELDRQQNQI
                     ELIASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYQGCAPSDAVETLAIERAKQLFNC
                     GFANVQPHSGAQANGAVMLALAKPGDTILGMSLDAGGHLTHGAKPALSGKWFNAVQYG
                     VRRDDDLIDFDQVEALAKEHRPTLIIAGGSAYPRHIDFARFRAIADEVGAIFMVDMAH
                     FAGLVAAGEHPTPFGHAHVVTTTTHKTLRGPRGGMVLTDDEAIARKINSAVFPGLQGG
                     PLMHVIAAKAVAFGEALQPEFKSYARAVIANAKALAGRLKERGADLVSGGTDTHVALV
                     DLRPLGVTGKDADEALERASITCNKNGIPFDPLPPVKTSGIRVGSPAGTTRGFGVAEF
                     RDIADMVADVLEGLRDKGEHGDPAVEAEVRRRVRKLCARFPIYQSPLPFRGEG"
     gene            5029..5490
                     /gene="nrdR"
                     /locus_tag="VF547_01845"
     CDS             5029..5490
                     /gene="nrdR"
                     /locus_tag="VF547_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX8261595.1"
                     /translation="MRCPFCSHENSQVKDSRPTEDGASIRRRRQCEECSARFTTFERV
                     QLRDLTVVKAGNRREPFDRGKLERSIAIACRKRPVPAERIDRLVSSLQRQIETSGDSE
                     IPSTRIGELVMDGLKALDPVAYIRFASVYKDFREAKDFEEFAGSVGGGESL"
     gene            5759..6871
                     /locus_tag="VF547_01850"
     CDS             5759..6871
                     /locus_tag="VF547_01850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HEX8261596.1"
                     /translation="MDFYAYLLRCSDGHYYVGHTDDLDARVAQHQSGAVPGYTQKHRP
                     VQLVWADRFPDRDSALAAERQMKGWSRAKKEALIRGDWEALRELAKRKPVLREAASPS
                     SAAPQDERSGLPPAHPEEGLSPGEGPSRRTPAIVLVRPQLGENIGKAARAMLNFGLTD
                     LRLVAPRDGWPNPSAGPAASGADLVLERARVFDSVAEAVADCAHVYATTVRKRGLVVP
                     VVTPEEAAREIRAQSEPSAILFGAERSGLETDEVAVAGKIVTVPVNPEFRSLNLAQAV
                     ILLAYEWSKLEALAVPTEGDAPEPRASQAQLEGLIGQLDEALEAAGYFHPPDRTPATR
                     NTIRTILTKAGWSNREVQALRGMIRALVEPRPGGRG"
     gene            complement(6882..7214)
                     /locus_tag="VF547_01855"
     CDS             complement(6882..7214)
                     /locus_tag="VF547_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX8261597.1"
                     /translation="MNANRTAPQACRTMAEVRHGVDRLDEMLVRLIGERFRYMEAAAR
                     IKPDRGQVRDEGRKAQVLANVARLAEEHGVPCAVAAELYERLIEASIAYELGRFDEIR
                     NGARTARE"
     gene            7305..7553
                     /locus_tag="VF547_01860"
     CDS             7305..7553
                     /locus_tag="VF547_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261598.1"
                     /translation="MRKLILIAALVAPTGTALADGGSAEANAKRNTQMVCRPIAETGS
                     RLSKKRVCMTREQWAEQKRIMRTDLNQAQTRRNEPKSE"
     gene            7731..8345
                     /gene="rpsD"
                     /locus_tag="VF547_01865"
     CDS             7731..8345
                     /gene="rpsD"
                     /locus_tag="VF547_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX8261599.1"
                     /translation="MSKRTSAKYKLDRRMGENVFGRPKSPVNRREYGPGQHGQRRKGK
                     MSDFGIQLRAKQKLKGYYGDVTEKQFKRTYTEAARMKGDTGQNLIGLLERRLDMVVYR
                     AKFAPTIWAARQLVSHGHIRVNGVKCNIASRRVNVGDEITLGNKAQEMALVMEAQGLA
                     ERDIPDYVVPDGTAKATFTRVPTLDEVPYPVRMEPNLVVEFYSR"
     gene            complement(8403..10364)
                     /locus_tag="VF547_01870"
     CDS             complement(8403..10364)
                     /locus_tag="VF547_01870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8261600.1"
                     /translation="MTAPLALLLLVGAGAPADPPAAFGVRERVLDISMSPGGSKIAFI
                     TPGPGQSTVLYTVDASGGKAPQRAVAADGRPERLTECGWVSEERLICTLYFVLENFVP
                     NQPLAGSRLIALNADGTDAKLLSRRVSANDLYLAVGGGEVIDWLPGSDGKVLIGRSYV
                     PESQIGTRFEEKRKGYAVDRIDTVSLRSDMVEQPDDHAGEYISDGRGNVRIKATFDVA
                     GATGYASGKYNYFYRTKGSRDWKPFSNYNVNTQEGLWPVAVDPQLDVAYGFRKDSSGR
                     QVLVSVALDGSKRETVVFAHPQVDVDSTLRIGRAQRVVGATFATEYRRAVFFDKELDA
                     LGRSLSKALPGLPLVAFVDSSEDESKLLLRAGSDTDPGRYYVFDKKSKTLNEIMLSRP
                     ELENVQLAEMKPITYKASDGTQVPAYLTLPPGSSGKGLPAIVMPHGGPSARDEWGFDW
                     LAQFFASRGYAVLQPNYRGSSGYGDAWYEKNGFQSWRSAIGDVNDAGRFLVAQGIAHP
                     AKLAIFGWSYGGYAALQANVLDPSLFKAAVAVAPVTDLATMIQDSIYWSNHRIVRNFI
                     GTGPHLKEGSPAENAGKIRAPVLLFHGDRDMNVGIRQSRIMADRLKDAGRPAELVVYP
                     NLDHSLEDSSARIDMLRRTDAFLKQALKL"
     gene            10504..12183
                     /locus_tag="VF547_01875"
     CDS             10504..12183
                     /locus_tag="VF547_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8261601.1"
                     /translation="MMVPLRSLAVAAAAALLLAGCATSRGRAPAAPPTNPAISLATLT
                     TVTQILSSDEYEGRAPTTAGEAKTVAYVADRFRKAGLQPGNKGSWFQDVPLVESTATP
                     TALTVTGGAAPIVLNQRADFVANSYQVQPKIDLRDSEMVFVGYGINAPERGWNDYQGV
                     DVRGKTVVILVNDPDWQDEDLEGPFNGKAMTYYGRWTYKYEEAARQGAAAALIVHDTE
                     PAAYGWNVVQSSWTGPQYNMDEPGGHMDQSKVIGWLTNEAARRVLAASGQDLDALSRA
                     ARQKGFRAVPLRTRASVSLANRIKKQASRNVIGVLPGAKRPNEYVIYTAHWDHLGRCD
                     ADKTGDDICNGALDNATGTAGLIALAEAHARRGNPERSIVFLAVTAEESGLLGSRYYA
                     EHPVYPLERTVGGINMDGLNVTGNTRDFVVIGKGKSELEDYMKRATDRFGLEIKNEPT
                     PQAGYYYRSDHFSLARGGVPMLYAEGGEDLVNGGTAAGRAAAEDYRRNRYHQPSDEYD
                     PSWDWSGAVRDLQIYWLMGRELADSTAWPNWHPGDEFRAIRDRSLAGAAPQ"
     gene            12180..>12607
                     /locus_tag="VF547_01880"
     CDS             12180..>12607
                     /locus_tag="VF547_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX8261602.1"
                     /translation="MTLRQPPEWAEHEWVWIGFPSHSDLWQDDLEPARREVAAFAAAV
                     HADGDGEEVRLVAADPASARAAAALAPFATVVQEPFGDIWLRDTGPIVLGRGAERRPR
                     SFRFNGWGGKYELEGDDTIGPRLAQGTALEARRFDWVLEGG"
BASE COUNT         2083 a         4457 c         4199 g         1868 t
ORIGIN      
        1 gccgcccacc cgaggctcgg tcccggcgag cagccggccg atgttcgagc gatggctcca
       61 gaagaccagc agggtgaagc cgaggaacag cagggccagg tcgaaccgcc cgagcagccc
      121 ggccgcgacc ggcatggcgg ccgccgcggc gaggccgccg agcgacgaat agcgggtggc
      181 gagaagcact ccgagccagg cggcggcgta ggcggccggc gccggccagc cgaggccatc
      241 gaccagcgcg aagccgagca ccaccccgaa ataggtcgcc acgcccttgc cgccggcgaa
      301 gcgcagccac accggccaga ggtggccgac catcgccccg atcgccgcca gctgcaccgt
      361 gccgggaagg aggcgatcgg cgaggacgag ggcggcggcg cccttggcca tgtcgagcag
      421 aagggtggcg gcggcgaggc ccttgcggcc ggtcctcagg acgttggtcg cgccgatatt
      481 gccggagccg acttgccgca ggtcgccggc gccgccgagc cgggtgagca ggattccgaa
      541 cgggatcgag cccagcgcat agccgagcag caacgcggcc aaaggtgcga cgatcgcttg
      601 catgtccacg acccctgctg aaagtttagc aaatcaacta atcaatgcaa tcgattcatc
      661 gaaacccggc aagcccgaat tcctccccgg gatttcccgg ggagggggga ccagccgaag
      721 gctggcggag gggcttcccg ccgaggcgga gaacccctcc accgcgcttc gcgcggtccc
      781 cctccccggc aaaggccggg gaggaattaa ggctcgcgtc cgcaaatcct ctgccgctaa
      841 acggggcgcg tgaccagtgg ccgcccactc ctcgtcttcg actccggcgt cggcggcctc
      901 tccgtcctcg gcgcgatccg cggcctgctg ccgaccgcgc ccgtggtcta cgccgccgac
      961 agcgccggct atccctacgg cacccggcgg ccggccgaga tcgaggcgcg cgtgccggcc
     1021 ctgctcggcc gcctcgccga gcgccacgat ccggagctga tcgtgatcgc ctgcaacacc
     1081 gcctcgacca tcgccctcga ttcggtccgg gcggcgctgg acctgccggt ggtcggaacc
     1141 gtgccggcga tcaaaccggc cgcgctcctg tcgacgaccc ggacgatcgg agtgctcggc
     1201 accgaggcga cggtccgcca gccctatgtc gaccggctct ccgccgagtt cgccgccgac
     1261 tgcacggtga tccgccacgg ctcggccgaa ctggtcgacc tcgccgaggc caagctgcgc
     1321 ggtgagccgc ccgacccgga ctccgtctcg gccgccctcg ccggcctgtt cgcccggccc
     1381 ggcggcggcc ggatcgacac cgtcgtcctc gcctgcaccc atttcccgct gatcgaggcg
     1441 gagctcgccg ccgccgcgcc ccatccggtc cgcttcgtcg acggcaagga ggggatcgcc
     1501 cgccgcaccg cctggctgac ccgcggccgg cgctggcccg aggccccttc ggagggcatc
     1561 gccctcttca ccggccgagg caccgacgtc gaggcctacc gccccggcct cgcccgctac
     1621 gggctgaccc ggatcgagcg gctgtaaata gcccctctcc cgcctttcgc gggagaggga
     1681 gattgagccg tctagccaat cccccgccaa atcggctaaa ggcggtcgaa gggacccccg
     1741 gggaaggcgc attggactat aaccgaatct tcgcgcaggc gatcgaccgg ctgcatgccg
     1801 agggccgcta ccgggtcttc atcgacatcc tcaggaaccg cggcaccttc cccaacgcgc
     1861 gctgcttcgc gggccacaac gggcccaagc cgatcaccgt ctggtgctcg aacgactatc
     1921 tgtgcatggg ccagcatccc gccgtggtcg aggcgatgga ggaagcgctg cacgacgtcg
     1981 gcgccggctc gggcggcacc cgcaacatcg gcggcaacac ccattaccat gtcgagctcg
     2041 aggccgagct cgccgacctc cacggcaagg aggcggcgct gctgttcacc tccggctaca
     2101 tctcgaacga ggcgactctg tcgaccgtcg ccaagctcct gcccggctgc atcatcttct
     2161 ccgacgagct caatcacgcc tcgatgattg ccgggatccg agcctcgggc tgcgagaagc
     2221 gcgtgttccg ccacaacgac ctggagcatc tcgaggccct gctcgccgag gccgacccgg
     2281 agtcgcccaa gctgatcgcc ttcgagagcg tctattcgat ggacggcgac gtcgccccga
     2341 tcggcgcctt ctgcgacctc gccgaccggt acaacgccat cacctatctc gacgaggtcc
     2401 acgccgtcgg catgtacggc ccgcgcggcg gcggcatctc cgagcgcgac gacgtcgccc
     2461 gccgggtgac catcatcgaa ggcacgctcg gcaaggcgtt cggagtgatg ggcggctata
     2521 tcgccgcgga caggaacatc gtcgacgtcg tccgaagcta cgcgccgggc ttcatcttca
     2581 ccacctcgct gtcgccggtg ctggtcgccg gggcgctggc gagcgtccgg cacctcaagg
     2641 cctcctgcgc cgagcgcgag gcccagcagg cgaatgccga gcgcctcaag gcgatcttcg
     2701 aggacgcgcg gctgccggtg atgccgtcga ccacccatat cgtgccgctg atggtcggcg
     2761 atccggtcaa ggccaagaag atcagcgaca tcctgctcgc cgaatatggc tgctacgtcc
     2821 agccgatcaa ctatccgacg gttccccgcg gcaccgagcg cctccgcttc accccggggc
     2881 cgcgccacga cgaggcgatg atgcgcgagc tggccaaggc gctggtcgag atctgggacc
     2941 gtctggagct tcgcgcggcg gcctgacgtc cttcgattcg gatcgtcgcc aagcccagcc
     3001 cctactcagg atgagcgggg cccactccat cccgctcgtc ctgagtagcc ggtgagcttg
     3061 gcgaaccggg ggatcgaagg accagacgct gcaatcactg gtggcaatcg cgtccgccat
     3121 cccctacaaa atgggcttca accccattgc cggagtccca tggccgagcg catcgccatc
     3181 gtatccgatc atgccgcgtt cgagatgaaa gccgccctcg ccgcttatct gcgcgacggc
     3241 ggccacgccg tgacggacct cgggccggac ggaatcgatt cggtcgacta tcccgattac
     3301 ggctacaagc tcgccgacga gatcgcggcg ggccgggccg acctgggagt ggccctctgc
     3361 ggctccggga tcggaatctc gatcgccgcc aaccgccatc ccgcctgccg ctgcgcgctc
     3421 gtatcggagc cgctgtcggc gcgcctcgcc cgcgagcata acgacgccaa cgcgatcgcc
     3481 atggggggcc gcctgctcgg cctcgagatg gcgaaggcct gtctcgacgc cttcctcgca
     3541 acgtcgttcg gcggcggccg ccaccagcgc cgggtcgaca agctttccaa ccccgccttc
     3601 gccagggaaa atgcatgagc agccgtagcg ccttcgattt cgccgaggtc cagcattcgg
     3661 gcttcttcac cggccggctc gccgaggccg atccggcggt cgccgaggcg gtcgccgccg
     3721 agctcgaccg gcagcagaac cagatcgagc tgatcgcgtc ggagaacatc gtctcgcgcg
     3781 ccgtgctcga ggcgcaggga tcggtcctga ccaacaaata tgccgagggc tatccgggcc
     3841 gccgctatta ccagggctgc gcgccttcgg atgccgtgga aacgctggca atagagcgcg
     3901 ccaagcaatt gtttaattgc ggcttcgcca acgtccagcc gcactcgggc gcccaggcga
     3961 acggcgcggt gatgctggct ctggccaagc cgggcgatac gatcctgggc atgtcgttgg
     4021 acgccggcgg ccacctcacc cacggcgcca agcctgccct ttcgggcaag tggttcaacg
     4081 cggtccaata cggagtccgg cgcgacgacg acctgatcga cttcgaccag gtcgaggcgc
     4141 tggccaagga acaccggccg acgctcatca tcgccggcgg ctcggcctat ccgcgccaca
     4201 tcgacttcgc ccggttccgc gccatagcgg acgaggtcgg cgcgatcttc atggtcgaca
     4261 tggcccattt cgccgggctc gtcgcggcgg gcgagcaccc gacccccttc ggtcacgccc
     4321 atgtcgtcac caccaccacc cacaagactc ttcgcggccc gcgcggcggg atggtcctga
     4381 ccgacgacga ggccatcgcc aggaagatca actcggcggt cttcccgggg ctccagggcg
     4441 ggcctctgat gcacgtcatc gccgccaagg cggtcgcctt cggcgaggcc ctgcagcccg
     4501 agttcaagag ctacgcgagg gccgtcatcg ccaacgccaa ggcgctcgcc ggccggctga
     4561 aggagcgcgg cgccgacctc gtctccggcg gcaccgacac ccatgtcgcg ctcgtcgacc
     4621 tgaggcctct cggagtcacc ggcaaggacg ccgacgaggc gctcgagcgc gcctccatca
     4681 cctgcaacaa gaacggcatc ccgttcgatc ccctgcctcc ggtcaagacc agcggaatcc
     4741 gggtcggctc gcccgccggc accacccgcg gcttcggggt cgccgagttc cgggacatcg
     4801 ccgacatggt cgccgacgtg ctcgaggggc ttcgcgacaa gggcgagcat ggcgacccgg
     4861 cggtcgaggc tgaggtgcgg cgccgggtgc gcaagctctg cgcccgtttc ccgatctatc
     4921 aatcccctct ccccttcagg ggggagggct agggagaggg gacgtgatgc cggccgcccc
     4981 ccgcctcccc ctctccccga ccctctcccc tgaaggggag agggagtagt gcgctgcccc
     5041 ttctgctccc atgagaacag ccaggtgaag gacagccgcc cgaccgagga cggagcctcg
     5101 atccggcgtc gtcgccagtg cgaggaatgc agcgcccgct tcacgacgtt cgagcgggtg
     5161 cagctgcgcg acctcaccgt ggtcaaggcc ggcaaccggc gcgagccgtt cgaccggggc
     5221 aagctcgagc gctcgatcgc catcgcctgc cgcaagcgcc cggtgccggc cgagcggatc
     5281 gaccgcctcg tctcctcgct ccagcgccag atcgagacca gcggcgacag cgagatcccc
     5341 tcgacccgga tcggcgagct ggtgatggac ggcctcaagg ccctggaccc ggtcgcctac
     5401 atccgcttcg caagcgtcta caaggacttc cgcgaagcga aggatttcga ggagttcgcg
     5461 gggagcgtgg ggggtggcga gagcctctga gcaggccttc gatacgtcct tcgatgcgtc
     5521 cttcgacacg tccttcgata cgccgcttcg acttcgctca gcggctactc aggatgagcg
     5581 ggagcgactg acctccatcg ctcatcctga gtagggactg agcctggcga aggcccgtat
     5641 cgaaggaccc gcgaaggtca catatgaact ctgtatccgg gcccgccccg ccttgtcccc
     5701 ctccgctcat cctgagtagg gactgagcga aggcgaaggc ccgtatcgaa ggacgctggt
     5761 ggatttctac gcttacctgc tgagatgctc cgacggccac tattatgttg gccataccga
     5821 cgatctcgac gcgcgcgtcg cgcagcacca aagcggagcc gttccgggct atacgcaaaa
     5881 gcatcggccg gttcagctgg tctgggccga ccgcttcccg gaccgcgaca gcgccctcgc
     5941 cgcggagcgc cagatgaagg ggtggagccg ggccaagaag gaagcgctga tccgaggcga
     6001 ttgggaagcg ctgagggaat tggcgaaaag gaagccggtc cttcgagagg ccgcttcgcc
     6061 aagctcagcg gctcctcagg atgagcggtc cggactgcct cccgctcatc ctgaggaggg
     6121 actgagcccc ggcgaaggcc cgtctcgaag gacccccgcc atcgtcctcg tccgccctca
     6181 gctcggcgag aatatcggca aggcggctcg ggcgatgctc aatttcgggc tgaccgacct
     6241 caggctggtg gcgccccgcg acggctggcc caatccttcc gccggaccgg cggcgtcggg
     6301 cgcggacctg gtcctggaac gggcgagggt gttcgactcg gtcgccgagg cggtggccga
     6361 ttgcgcacac gtctatgcca ccaccgtgcg caagcgcggc ctcgtcgtgc cggtggtgac
     6421 tccggaggag gcggcgcggg agattcgggc gcagtcggaa ccctcggcca tcctgttcgg
     6481 cgccgaacgc tcgggcctgg agacggacga ggtcgccgtc gccggcaaga tcgtcaccgt
     6541 gccggtcaat cccgagttcc ggtcgctcaa cctcgcccag gccgtcatcc tcctcgccta
     6601 cgaatggtcg aagctggagg cgcttgcggt tccgaccgaa ggcgatgcgc cggagccgcg
     6661 cgccagccag gcccagctcg aggggctgat cgggcagctc gacgaggcgc tcgaggccgc
     6721 cggctatttc cacccgcccg accggacccc ggcgacccgg aacacgatcc gcaccatcct
     6781 caccaaggcg ggctggtcga accgcgaggt ccaggcgctg cgcggcatga tccgagccct
     6841 ggtcgagccg cggccgggcg ggcgcggttg acgccttttg cttattcccg cgcggtccgg
     6901 gcgccgttcc tgatctcgtc gaaccggccc agctcgtagg cgatcgaggc ttcgatcagc
     6961 cgctcgtaaa gctccgccgc gaccgcgcag ggcacgccgt gctcctccgc caggcgggcg
     7021 acattggcga gcacctgcgc cttgcgcccc tcgtcgcgca cctgtccgcg gtccggcttg
     7081 atccgggcgg cggcctccat gtagcggaag cgctcgccga tcagccggac gagcatctcg
     7141 tcgagccggt cgaccccgtg gcggacttcg gccatcgtcc ggcaggcctg cggagccgtt
     7201 cggttcgcgt tcatgcgccc tgctttaacg ccgccgcgca gggtgtcgag cttgacttcg
     7261 gcgccgccag cggagaattt gccgcggact ggatggggat tgcgatgaga aagctgattt
     7321 tgatcgcggc gctggtggcg cccacgggga ccgcccttgc cgacggcggc agcgccgagg
     7381 ccaatgccaa gcgcaacacg cagatggtgt gccgcccgat cgccgagacc ggctcccgcc
     7441 tgtccaagaa gcgcgtctgc atgacccggg agcaatgggc cgagcagaag cgcatcatgc
     7501 gcaccgacct caaccaggcg cagacccgcc ggaacgagcc caagtccgaa tagccggacg
     7561 acccttcaac aggcctggaa gcggcgccgc ggttgacttt cgcggcccgc gccgccatgt
     7621 gcggcgcttc gcaattggcc ccgcaccccg gtgaagcggt ggccgcgctt gggaaatttc
     7681 ccctgcggtg cggttccggg acggacgaca acagcagatt ggaccgatcg atgagcaagc
     7741 gcaccagcgc caagtacaag ctcgaccggc gaatgggcga gaacgtcttt ggacggccca
     7801 agagcccggt caaccgccgc gaatacggcc ccggacagca cggccagcgc cgcaagggca
     7861 agatgtcgga cttcggcatc cagctccgcg ccaagcagaa gctgaagggc tattacggcg
     7921 acgtcaccga gaagcagttc aagcgcacct acaccgaggc cgcgcggatg aagggcgaca
     7981 ccggccagaa cctgatcggc ctgctcgagc gccgtctcga catggtcgtc taccgcgcca
     8041 agttcgcgcc gacgatctgg gcggcgcgcc agctggtcag ccacggccac atccgggtca
     8101 acggggtgaa gtgcaacatc gcctcgcggc gggtcaacgt cggcgacgag atcaccttgg
     8161 gcaacaaggc gcaggagatg gcgctggtga tggaggcgca gggcctcgcc gagcgcgaca
     8221 ttcccgacta cgtcgtcccg gacggcaccg ccaaggcgac cttcacccgc gttccgacgc
     8281 tggacgaggt cccctatccg gtgcgcatgg agccgaacct ggtcgtcgag ttctactcgc
     8341 gctgaggctt ctgacgagag ctgcgaacga ggggcggcgc cgcgaggggc cgcccctttt
     8401 cgctacagct tcagagcctg cttaaggaag gcgtccgtgc gccggagcat gtcgatgcgg
     8461 gcactgctgt cctcgaggct atggtcgagg ttcgggtaga cgaccagttc ggccgggcgg
     8521 ccggcgtctt tcaggcgatc ggccatgatc cgcgactgcc ggatgccgac gttcatgtcc
     8581 cggtctccgt ggaagaggag gaccggcgcc ctgatcttgc cggcgttctc ggccggcgat
     8641 ccttccttga ggtgcgggcc ggtgccgatg aagttgcgca cgatccgatg gttcgaccag
     8701 tagatcgaat cctggatcat cgtcgcaagg tcggtgaccg gcgccacggc caccgcggcc
     8761 ttgaacaggc tcggatcgag gacgttggcc tgcagcgccg catagcctcc gtaggaccag
     8821 ccgaagatcg ccagcttggc cggatgggcg atgccctggg cgacgagaaa gcgcccggcg
     8881 tcgttgacgt cgccgatcgc cgagcgccac gactggaaac cgttcttctc gtaccacgcg
     8941 tcgccatagc ccgaggaacc gcgataattg ggttggagca cggcatagcc gcggctggcg
     9001 aagaactggg cgagccagtc gaagccccat tcgtcgcgcg cgctcgggcc gccgtgcggc
     9061 atcacgatgg ctggaagacc cttgccgctg cttcccgggg gcagcgtcag ataggccggc
     9121 acctgggtgc cgtccgaggc cttgtaggtg atgggcttca tctcggcgag ctggacattc
     9181 tccagctccg gccgcgacag catgatctcg ttcagggtct tggacttctt gtcgaagaca
     9241 taatagcggc caggatcggt atcgcttccc gcccgaagca ggagcttgct ctcgtcctcg
     9301 ctcgaatcga cgaaggcgac gagcggcaat ccgggcaagg ccttggacag cgagcggccg
     9361 agcgcgtcca gctccttgtc gaagaatacg gcgcggcgat attcggttgc gaaggtcgcg
     9421 ccgacgacgc gctgggcgcg gccgatgcgc agggtcgagt cgacgtccac ctgcggatgg
     9481 gcgaagacga cggtctcgcg cttcgagccg tcgagcgcga cgctgaccag gacctggcga
     9541 ccggacgagt ccttgcggaa gccgtaggcg acgtcgagct gcggatcgac ggcgaccggc
     9601 cagaggccct cctgcgtgtt cacgttgtag ttcgagaagg gcttccagtc gcgcgagccc
     9661 ttggtccgat agaaataatt gtacttgccg ctcgcgtaac cggttgctcc ggcgacatcg
     9721 aacgtcgcct tgatccggac gttgcctcga ccgtcgctga tatattcgcc ggcatggtcg
     9781 tcgggctgct cgaccatgtc ggagcgcagc gagaccgtgt cgatccggtc gaccgcataa
     9841 cccttcctct tttcctcgaa acgggtgccg atctgggatt cgggcacata gctccggcca
     9901 atcaggacct tgccgtcact gcccgggagc cagtcgatca cttcgccgcc gccgaccgcg
     9961 agatagaggt cgttggcgct gacccggcgg ctgagcagct tggcgtcggt gccgtcggca
    10021 ttgagcgcga tcaggcgcga gccggcgaga ggctggttcg ggacgaagtt ctcgaggacg
    10081 aaatagaggg tgcagatcag gcgctcctcg gagacccagc cgcattcggt cagccgctcg
    10141 ggtcgcccgt cggccgcgac ggccctctgc ggcgcctttc cgccgctggc atcgaccgtg
    10201 tagaggacgg tcgactggcc gggccccggg gtgatgaagg cgatcttcga tccgcccggc
    10261 gacatcgaga tgtcgagcac gcgttcgcgg acgccgaatg cggccggcgg atcggccggc
    10321 gcgcccgcgc ccacgaggag aagcagcgca agcggcgccg tcatgaatat cctaaacatg
    10381 aataccccca gtcacttagg cccctatccc aagtggcggg aggatggcac cgcatccgtc
    10441 cgcgcgcaag cttgcgtgcg ccgaatcttg ctgtcatggg gcgcccgagg gggaagagga
    10501 gacatgatgg tgccgcttcg atcccttgcc gtcgccgccg ccgcggcctt gctgctcgcc
    10561 ggctgcgcca cgtcgcgcgg ccgcgccccg gccgctcccc cgaccaatcc ggccatctcg
    10621 ctggcgaccc tcaccaccgt cacccagatc ctctcctccg acgaatatga gggccgcgcc
    10681 ccgaccactg cgggcgaggc gaagacggtc gcctatgtcg ccgaccgttt ccgcaaggcc
    10741 ggcctgcagc cgggcaacaa gggaagctgg ttccaggacg tgccgctggt cgagagcacc
    10801 gccaccccga ccgccctgac cgtcaccggc ggcgccgcgc cgatcgtcct caaccagcgc
    10861 gccgatttcg tcgccaattc ctaccaggtg cagccgaaga tcgacctcag ggacagcgaa
    10921 atggtcttcg tcggctacgg catcaacgcg cccgagcgcg gctggaacga ttatcaaggc
    10981 gtcgacgtgc gggggaagac ggtcgtcatc ctggtcaacg atccggactg gcaggacgag
    11041 gacctcgaag gccccttcaa cggcaaggcg atgacctatt acggccgctg gacctacaaa
    11101 tatgaggaag cggcacggca gggcgccgcc gccgcgctga tcgtccacga caccgagccg
    11161 gccgcctatg gctggaacgt cgtccagtcg tcctggaccg ggccccaata caatatggac
    11221 gagcccggcg gccacatgga ccagtcgaag gtgatcggct ggctcaccaa cgaggcggcg
    11281 cgccgggtgc tcgcggcctc cggccaggac ctcgacgccc tctcgcgggc cgccaggcag
    11341 aagggcttca gggccgttcc gctgcgcacc cgggcgtccg tctcgctcgc caaccggatc
    11401 aagaagcagg cgtcgaggaa cgtcatcggc gtcctgcccg gcgcgaagcg gccgaacgaa
    11461 tatgtgatct acaccgccca ctgggaccat ctcggccgct gcgacgccga caagaccggc
    11521 gacgacatct gcaacggcgc gctcgacaat gcgaccggga ccgccggcct catcgctctc
    11581 gccgaagccc atgcccggcg cggcaatccc gagcgctcga tcgtcttcct cgccgtcacc
    11641 gccgaggaat ccggcctgct cggctcccga tattatgccg agcaccccgt ctatccgctc
    11701 gagcgcaccg tcggcgggat caacatggac ggcctcaacg tgaccggcaa cacgcgcgac
    11761 ttcgtcgtca tcggcaaggg caagtccgag ctcgaggact atatgaagcg ggcgacggat
    11821 cggttcggcc tggagatcaa gaacgagccg acgccgcagg ccggctatta ttaccgctcc
    11881 gaccatttca gcctcgcccg cggcggcgtg ccgatgctct atgccgaggg cggcgaggac
    11941 ctggtcaacg gcggcacggc ggcgggccgc gccgccgccg aggattatcg ccgcaaccgc
    12001 taccaccagc cgagcgacga atatgacccg tcctgggact ggtcgggggc ggtccgcgac
    12061 ctccagatct actggctgat gggccgcgag ctggccgatt cgaccgcctg gcccaattgg
    12121 catccgggcg acgagttccg ggccatccgc gaccgaagcc tcgccggcgc cgcgccgcaa
    12181 tgacgttgcg ccagccgccc gaatgggcgg agcatgagtg ggtttggatc ggctttccga
    12241 gccactcgga cctgtggcag gacgatctcg agccggctcg gcgcgaggtg gccgccttcg
    12301 ccgccgcggt ccatgccgac ggcgacggcg aggaggtgcg gctggtcgcc gccgaccccg
    12361 cttcggcccg cgccgccgcc gccctcgccc ccttcgccac cgtggtgcag gagccgttcg
    12421 gcgacatctg gctgcgcgac actgggccga tcgtcctcgg ccgcggagcg gagcggcggc
    12481 cgcgcagctt ccgcttcaac ggctggggcg ggaaatacga gctcgaaggc gacgacacga
    12541 tcgggccaag gctcgcacag ggcaccgccc tcgaggcgag gcgcttcgac tgggtcctgg
    12601 aaggcgg
//
LOCUS       DASVQZ010000069         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_591867, whole genome shotgun sequence.
ACCESSION   DASVQZ010000069 DASVQZ010000000
VERSION     DASVQZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591867"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VF547_01885"
     CDS             <1..1001
                     /locus_tag="VF547_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX8261603.1"
                     /translation="QHVDDSAYAGFLADRIASALAAQGVPAPDILPPHISPPNSRRRA
                     SLRADRGGALGFNAEASHRIVDMRECHILRPELFALVAPLRGLLRALKASATAGVRLT
                     LADQGVDLLLEKVEADGLAAAEAVGAFAADHRLARLALDDGYGAQTRWEPVPVTITLG
                     GVPVPLPHNAFLQATAEGEGALVEAVRRAVGPARIVADLFAGLGTFALALEGRVLAVE
                     GARDAAIALKAGAGRAQRQVFVDHRDLYRRPLDPAELNRFEAAVLDPPRAGAKEQSAA
                     IARSEVPRIAYVSCNPSTFARDAATLIEGGYRFDWALPVGQFRWSTHVELVGAFSR"
     gene            complement(998..1438)
                     /locus_tag="VF547_01890"
     CDS             complement(998..1438)
                     /locus_tag="VF547_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8261604.1"
                     /translation="MNSPILAPVVALVGWTLLVMIWMMAARFAAMKRKNISLKGRVGG
                     RGGTQLEGVVEDQVMWKAHNYMHLVEQPTLFYAIALALALGHAGGGNSLILAWAYVGL
                     RVAHSLVQATVNVVAYRFLLFALSSLALIGLTIQGIRMMFSPLY"
     gene            complement(1515..>2359)
                     /locus_tag="VF547_01895"
     CDS             complement(1515..>2359)
                     /locus_tag="VF547_01895"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX8261605.1"
                     /translation="STSAGGCMDLYADLIKHNMVDAVVATGATIVDMDFFEALGHKHY
                     QALEIPDDDTLRSLYIDRIYDTYIDEESLQETDHTIGAIADSLEPRPYSSRAFIREMG
                     KWLVENGKKENSLVKLAYEHDVPIFCPAFTDSSAGFGLVKHQVDAMKAGRPYLTMDSI
                     ADFRELTDIKIKAGTSGLLMIGGGVPKNFVQDTVVCAEILGHDDVEVHKYAVQITVAD
                     VRDGACSSSTLQEAASWGKVSTAMEQMVFAEATSVLPLLASDAYHRGHWQGRTKRRFA
                     KLFE"
BASE COUNT          362 a          810 c          796 g          391 t
ORIGIN      
        1 tccaacatgt cgacgactcg gcctatgccg gcttcctggc cgatcggatc gcctcggcgc
       61 tggccgcgca gggcgtcccg gcccccgaca tccttccgcc ccatatctcg ccgcccaaca
      121 gccgccggcg cgcgtcgctg agggcggacc ggggcggagc gctcggcttc aacgccgagg
      181 ccagtcaccg gatcgtcgac atgcgcgaat gccatatcct tcgtccggag ctgttcgccc
      241 tcgtcgcgcc gctgaggggg ctgcttcgcg ccctgaaagc ctcggcgacc gccggcgtgc
      301 gcctgaccct cgccgaccag ggcgtcgacc tgctgctcga gaaggtcgag gcggacggcc
      361 tggcggcggc cgaggccgtg ggcgccttcg ccgccgacca tcgcctcgcc cggctggcgc
      421 tggacgacgg ctacggcgcc cagacccgct gggagccggt gccggtcacg atcacgctcg
      481 gcggagtgcc ggtgccgctc ccgcacaatg ccttcctcca ggcgacggcg gagggggagg
      541 gcgcgctcgt cgaggcggtg aggcgcgccg tggggccggc ccggatcgtc gccgacctgt
      601 tcgccgggct cggcaccttc gcgctcgccc tcgagggccg ggtgctcgcg gtcgaaggcg
      661 ctcgcgacgc cgcgatcgcc ctcaaggccg gggccggccg ggcgcagcgt caggtcttcg
      721 tcgaccatcg cgacctctac cgccgcccgc tcgacccggc ggagctgaac cgcttcgagg
      781 cggcggtgct cgatccgccg cgcgccggcg cgaaggagca gtcggcggcg atcgcgcgct
      841 ccgaggtccc gcgcatcgcc tatgtgtcgt gcaatccctc gaccttcgcc cgcgacgccg
      901 cgacgctgat cgagggcggc taccgtttcg attgggcgct ccccgtcggc cagttccgct
      961 ggtcgaccca cgtcgagctg gtcggagcgt tcagccgcta atagagcggg ctgaacatca
     1021 tcctgatccc ctgaatcgtg aggccgatca gcgccagcga cgacaaggcg aacaggagga
     1081 agcgataggc gacgacgttg accgtcgcct gcaccaggct gtgcgccacc cgcagtccca
     1141 cataggccca ggcgagaatc aggctgttgc cgccgccggc atggccgagg gccagcgcca
     1201 gcgcgatcgc gtagaagagc gtcggctgct cgaccaggtg catgtagttg tgcgccttcc
     1261 acatcacctg gtcctcgacg acgccttcga gctgggtgcc gccgcggccg ccgacccggc
     1321 ccttcaatga gatgttcttg cgcttcatcg ccgcgaagcg cgcggccatc atccagatca
     1381 tgaccagcag cgtccagccg acgagcgcga cgaccggtgc gagtattgga ctgttcatgg
     1441 cgatcctccc ccttgagagg gggagggtgc gtcataatgg gcccggattc aattcctccc
     1501 ggcgccggag gaggctattc gaacagcttc gcgaagcggc gcttcgtccg gccctgccaa
     1561 tggccgcggt gataggcgtc cgacgccagc aggggcagga cgctggtcgc ctcggcaaag
     1621 accatctgct ccatcgccgt cgacaccttg ccccagctcg ccgcttcctg gagggtcgag
     1681 gaggagcagg ccccgtcgcg gacgtcggcg acggtgatct ggaccgcata tttgtggacc
     1741 tcgacgtcgt cgtggccgag gatctcggcg cagacgaccg tgtcctgcac gaaattcttg
     1801 ggcaccccgc cgccgatcat cagcaggccc gacgtgcccg ccttgatctt gatgtccgtc
     1861 agctcgcgga agtcggcgat cgaatccatc gtcagatagg gccggcccgc cttcatcgcg
     1921 tcgacctgat gcttgaccag gccgaagccc gccgagctgt cggtgaaggc gggacagaag
     1981 atcggaacgt catgctcata ggcgagcttg acgaggctgt tctccttctt gccgttctcg
     2041 accagccact tgcccatctc gcggatgaag gcgcgcgacg agtaggggcg gggctccagc
     2101 gaatcggcga tcgctccgat cgtatgatcg gtctcctgca gcgattcctc gtcgatatag
     2161 gtatcgtaga tgcggtcgat atagagcgaa cggagcgtat cgtcgtcagg gatttcaaga
     2221 gcttggtagt gcttgtggcc tagcgcttcg aagaaatcca tgtcgacgat cgtggcaccg
     2281 gtggcgacga ctgcatcgac catgttgtgc ttgatgaggt ccgcatagag atccatgcag
     2341 ccgccggcgg aggtcgagc
//
LOCUS       DASVQZ010000070         6882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_15416, whole genome shotgun sequence.
ACCESSION   DASVQZ010000070 DASVQZ010000000
VERSION     DASVQZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15416"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..865
                     /gene="ccmC"
                     /locus_tag="VF547_01900"
     CDS             152..865
                     /gene="ccmC"
                     /locus_tag="VF547_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX8261606.1"
                     /translation="MHILANPTRFLRLARPLTAWLGWGGALLIAAGLGWGLFVAPPDY
                     LQGEAVRIIYIHVPAAWLGMAGWAGIAVASLMQIVWRHPLAAVAVRALAVPGAVFAFI
                     CLATGSLWGRPAWGTWWEWDGRMTSMLVLLFLYVGLIALAEAERERGGEGRLTAVFGL
                     VGAINLPIIHYSVLWWRTLHQGQSIGLTRSSIDGSMLWPLLASALGFSLLFGAVVLMR
                     MRTALAVQKVEARMRRMAG"
     gene            862..993
                     /locus_tag="VF547_01905"
     CDS             862..993
                     /locus_tag="VF547_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX8261607.1"
                     /translation="MTHWPFILGAYAVALIGTLGLTGWSFAAMRRAERAVDALKDER"
     gene            990..1430
                     /gene="ccmE"
                     /locus_tag="VF547_01910"
     CDS             990..1430
                     /gene="ccmE"
                     /locus_tag="VF547_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX8261608.1"
                     /translation="MKAKNQRLTLLVLAVAAVLGAVLLAMSALRDQAAYFYAPGDVAR
                     GAPPAGRSVRIGGMVQAGSLKRAPDGVTIEFLVRDETPHRIRVRFRGIVPDLFKENSG
                     VVAEGRFEAGGLFVADNILAKHDENYMPPQLDRKKGEHKSETLK"
     gene            1625..3463
                     /locus_tag="VF547_01915"
     CDS             1625..3463
                     /locus_tag="VF547_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX8261609.1"
                     /translation="MPERNFASAIRPVAVVQALLVAAAFLALVRLFLRTDLSVALVAA
                     NSHSMKPWLYKLAGAWGNHEGSMLLWVTVLAVAGAAVALFERRLEERTLIATLAAQAA
                     IGLGFYAFLLFSSNPFERLVPAPAEGNGLNPLLQDPGLAFHPPTLYFGYVGVSIAFSF
                     AVGALVTRDVGPVFARAMRPWVLGAWIFLTLGITAGSYWAYYELGWGGWWFWDPVENA
                     SLMPWLAATALLHSVTVLATRDGLRAWTVMLAVVAFSMSMVGTFLVRSGILTSVHAFA
                     VDPERGAFILLLLAFYIGGALTLFGLRVGTVKEGSTFEPVSREGALVLNNLLLSAVLG
                     IVLVGTLYPLIAEAATGEKLSVGPPYFNTAAGPLALALVAAMAAGPLLKWRRDRLREV
                     AGRIALPILVAALALLALVLLVPGLRLLPLLGLVLALGVGAASLAPLWKRNLRRTPLF
                     TWGMVIAHLGIAVALAGMASESAFSREKLVAARVGESVAVGPWTLRFDSVEPVAGPNW
                     TAVEARLTARRGGGAPFVLLPQARMFSSPPTETSEAAIATRLDGQLYTVIGKPDGGGR
                     WQLRLWWKPFVTLIWLGGGLVALGGLLALVGRIRRGRRQAPAEAWA"
     gene            3460..3987
                     /locus_tag="VF547_01920"
     CDS             3460..3987
                     /locus_tag="VF547_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX8261610.1"
                     /translation="MKRLLLWLPLALFAAFVVTVAIGLRKPADTQIRSRMIGKPMPDF
                     ALEPAVAGHPPLAAVDLRRGKPRLVNVFASWCVPCKVEAPQLEALKRRGVPIDGIAIR
                     DRSEDVARFLAEWGDPFDRIGADPASKVQFALGSSGVPETFVVDGRGVIRHQHIGDIR
                     PEHVAQIVAAYEAAK"
     gene            4050..4469
                     /locus_tag="VF547_01925"
     CDS             4050..4469
                     /locus_tag="VF547_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX8261611.1"
                     /translation="MLRFARNDGAAILLALAAPAFAQSSLPPSRYAYTQLEDARQEAE
                     AKALMETLRCLVCQGQSIADSDADMAGDMRALVRERIAAGEKPEAIRRWLIDRYGAWV
                     SYRPPVEPLTWPLWAAPLLLFAAGLYLARGRFRRKPR"
     gene            4469..5095
                     /locus_tag="VF547_01930"
     CDS             4469..5095
                     /locus_tag="VF547_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8261612.1"
                     /translation="MGWLILLLLGLAAFAAIWRFGRLDGAGAQLLAAALLLGFAGYAW
                     QGRPGLAGSPKAPPASQALPDSAFARMRQDMLGRFDTADRWLTIAEGYQRRGDTRAGA
                     GLIRAALREHPDNAILWIGYGNALVIHAGGMITPAAQLAFERAGQLAPDHPAPRFFFG
                     VALAQSGRLDEAERTWRALIAAAPPDAAWRAGVEAQLQQLEQARAAGR"
     gene            complement(5247..5459)
                     /locus_tag="VF547_01935"
     CDS             complement(5247..5459)
                     /locus_tag="VF547_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX8261613.1"
                     /translation="MSTQQTPPPTPEKDPEEWVTGDESMTGPQASYLKTLSQEAGEEF
                     DPGLSKAEASKRIDELQHETGRGIDH"
     gene            complement(5525..6625)
                     /gene="ald"
                     /locus_tag="VF547_01940"
     CDS             complement(5525..6625)
                     /gene="ald"
                     /locus_tag="VF547_01940"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX8261614.1"
                     /translation="MRVGVPKEIKNHEYRVGLTPGSVAELTGAGHEVIVETKAGMGID
                     FDDQAYVDAGARIAPDAASTFREADMIVKVKEPQAREIALLEPRHLLFTYLHLAPDRP
                     QTEGLMKSGATCIAYETVTAADGSLPLLKPMSEVAGRMSIQVGAHYLEKEQGGRGVLL
                     GGVPGVAPARVAILGGGVAGVNAAQMAVGMRADVTIYDISNARLAELDMFFGSQIKTA
                     YSSRAAIAHAVSKCELVIGAVLVPGAAAPKLVTRDMLKTMKRGSVLVDIAIDQGGCFE
                     TSRATTHDDPVYEIDGVIHYCVANMPGAVARTSAFALNNATLPFVLKLAGQGAEVAMK
                     SDPHLANGLNVSGGKIRHRAVAEALDLPFEAA"
     gene            6806..6882
                     /locus_tag="VF547_01945"
                     /pseudo
     CDS             6806..6882
                     /locus_tag="VF547_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
BASE COUNT          885 a         2453 c         2382 g         1162 t
ORIGIN      
        1 caccacccgg ggtcctgcca tgccgcgagc gccacctcta cgagggggcc gtatgcgccg
       61 gccggcagcc cgcacgcttc gccaaaatgg ggcgtgtgcc gctgctacgg ctttgagccc
      121 gggtccggct ccgctaagga cgcagcgggc aatgcatatt ctcgcgaatc cgacacgctt
      181 cctgcggctt gcgcggccgc tcactgcctg gctcggctgg ggcggggcgc tgctgatcgc
      241 cgccggcctg ggatgggggc tgttcgtcgc gccgcccgat tatctccagg gcgaagccgt
      301 gcgaatcatc tacatccacg tgccggccgc ctggctcggc atggcgggct gggcgggcat
      361 cgcggtcgca agcctgatgc agatcgtctg gcggcacccg ctcgcggcgg tggcggtccg
      421 agcgctcgcc gtgccgggag cggtcttcgc cttcatctgc ctcgccaccg gatcgctttg
      481 gggacggccc gcctggggca cctggtggga atgggacggg cggatgacgt cgatgctcgt
      541 gctgctcttc ctctacgtgg gattgatcgc ccttgccgag gccgagcgcg agcgcggcgg
      601 cgaggggcgg ctcacagccg tcttcggcct cgtcggagcg atcaacctgc cgatcatcca
      661 ttattcggtc ctgtggtggc gaaccctgca ccaggggcag agcatcggcc tgacccgatc
      721 cagcatcgac ggctcgatgc tctggccgct gctggcgtcg gcgctcggct tcagcctgct
      781 gttcggagcg gtcgtgctga tgcggatgcg caccgcgctg gcggtgcaga aggtcgaggc
      841 gcggatgcgc cggatggccg gatgacccat tggccgttca tcctcggcgc ctatgcggtg
      901 gccctgatcg gcacgctcgg cctgaccggc tggagcttcg ccgccatgcg ccgcgccgaa
      961 cgggcggtgg acgccctgaa ggatgaaagg tgaaggcgaa gaaccagcgc ctgacgctgc
     1021 tcgtcctcgc cgtcgccgcg gtgctcgggg cggtgctgct cgccatgtcc gccctcaggg
     1081 accaggcggc atatttctac gcccccggcg acgtcgcccg gggagcccct ccggccgggc
     1141 gctccgtccg catcggcggc atggtccagg ccggatccct gaagcgcgcg cccgacggcg
     1201 tcaccatcga gttcctcgtc cgggacgaga ccccgcacag gatccgagtc cgcttccgcg
     1261 gcatcgtccc cgatctcttc aaggagaata gcggagtcgt cgccgaaggc cggttcgagg
     1321 ccggcgggct gttcgtcgcc gacaacatcc tcgccaagca tgacgagaat tacatgcccc
     1381 cgcagctcga ccgcaagaaa ggcgagcaca agagcgagac cctgaaatga tcgccgaggc
     1441 aggcctggcg gccttgtggc tggcggcggc gctgtcgctg ctccagctcg ggctggggat
     1501 cgcggcactc ttttcctccc ctgcatttgc aggggagggg gaccgggcgg agcgcggtgg
     1561 aggggttttt cagagtcagc aagaacccct ccaccaggcc tcgcctggtc cccctccccg
     1621 gcaaatgccg gagaggaatt ttgcgtccgc catccgtccc gtcgccgtcg tgcaggcgct
     1681 gctcgtcgcc gccgccttcc tcgccctcgt ccggctgttc cttcgcaccg acctttccgt
     1741 cgccctggtc gccgccaaca gccattcgat gaagccctgg ctctacaagt tggccggcgc
     1801 ctggggcaat cacgaggggt cgatgctgct gtgggtgacc gtgctggccg tggcgggcgc
     1861 cgcggtcgcg ctgttcgagc gccggctgga ggagcggacg ctgatcgcga ccctggcggc
     1921 gcaggcggcg atcggccttg gattctatgc gtttctgctc ttttcctcaa acccgttcga
     1981 gcggctcgtc ccggccccgg ccgagggcaa cggcctcaac cccctgctcc aggaccccgg
     2041 cctcgccttc catccgccga ccctctattt cggctatgtc ggggtctcga tcgctttctc
     2101 cttcgcggtg ggagcgctcg tcacccgcga cgtcggtccc gtcttcgccc gtgcgatgcg
     2161 gccgtgggtg ctcggcgcgt ggatcttcct gacgctgggg atcaccgccg gctcctattg
     2221 ggcctattac gagctcggct ggggcggctg gtggttctgg gacccggtgg agaacgcctc
     2281 gctcatgccc tggctcgcgg cgacggccct gctccattcg gtgaccgtgc tcgccacccg
     2341 cgacggcctt agagcctgga cggtgatgct ggccgtggtc gccttctcga tgtcgatggt
     2401 cggcaccttc ctggtccgct ccggcatcct gaccagcgtg cacgccttcg ccgtcgatcc
     2461 cgagcggggg gccttcatcc tcctgctgct cgccttctac atcggcggcg ccctgacgct
     2521 gttcggcctt cgggtcggga cggtgaagga gggctcgacc ttcgagccgg tcagccgcga
     2581 gggcgcgctg gtgctcaaca accttctgct ctcggccgtg ctcggaatcg tcctcgtcgg
     2641 gacgctctat ccgctgatcg cggaggccgc gaccggcgag aagctctcgg tcggcccgcc
     2701 ctacttcaac acggccgccg gcccgctcgc gctcgctctg gtggcggcga tggcggctgg
     2761 gccgctgctc aaatggcggc gcgaccggct gcgcgaggtg gccggacgga tcgctctgcc
     2821 gatcctcgtc gccgcgctcg cgctgctggc gctggtgctg ctcgtgccgg gcctgcgcct
     2881 gctgccgctg ctcggcctgg tcctggcgct cggcgtcggc gcggccagcc tggcgccgct
     2941 gtggaagcgc aacctgcggc ggacgccgct gttcacctgg ggcatggtga tcgcccatct
     3001 cggaatcgcc gtcgctctgg ccggcatggc gtcggagagc gcgttcagca gggagaagct
     3061 ggtcgccgcc cgggtcggcg aatcggtggc ggtcggaccc tggaccctgc gcttcgactc
     3121 ggtcgagccg gtggccggac cgaactggac ggcggtcgag gcccggctca ccgcccgccg
     3181 cggcggcggc gccccgttcg tcctgctgcc gcaggcgcgc atgttcagca gcccgccgac
     3241 cgagaccagc gaggcggcga tcgccacccg gctcgacggc cagctctaca ccgtgatcgg
     3301 caagccggac gggggcggcc gctggcagct cagattgtgg tggaagccgt tcgtcaccct
     3361 gatctggctg ggcggcggcc tcgtcgcgct cggcggactg ctcgctttgg tcggtcggat
     3421 ccgccgcggg cggcgccagg cccccgcgga ggcctgggca tgaagcgcct gctcctctgg
     3481 ctgccgctgg ccctgttcgc ggccttcgtc gtcaccgtcg cgatcgggct ccggaagccg
     3541 gccgacaccc agatccgatc gcgcatgatc ggcaagccga tgcccgattt cgcgctggag
     3601 cccgcggtcg ccggccaccc gccgctcgcc gccgtcgatc ttcgccgggg caagccgcgc
     3661 ctcgtcaacg tcttcgccag ctggtgcgtg ccatgcaagg tcgaggcgcc gcagctcgag
     3721 gcgctcaagc ggcgcggggt tccgatcgac gggatcgcca tccgcgaccg gagcgaggac
     3781 gtcgcccgct tcctcgccga atggggcgat cccttcgatc ggatcggggc ggatccggcc
     3841 agcaaggtcc agttcgcttt gggctcgtcg ggcgtcccgg agaccttcgt cgtcgacggc
     3901 cgcggcgtca tccgccacca gcatatcggc gacataaggc cggagcatgt ggcgcagatc
     3961 gtcgccgctt acgaggcggc gaaatgatcc cgtcattgcg aggagcgaag cgacgcggca
     4021 atccagcggg ccccggaggc cgctctggat tgcttcgctt cgctcgcaat gacggggctg
     4081 caatccttct cgcgctcgcc gcccccgctt tcgcccaatc gtccctgccg ccgtcgcgct
     4141 acgcctatac ccagctcgag gatgcgcggc aggaggccga ggccaaggcg ttgatggaaa
     4201 ccctgcgctg cctcgtctgc cagggccagt cgatcgccga ttcggacgcc gacatggccg
     4261 gggacatgcg cgctctggtg cgcgagcgga tcgccgcggg cgagaagccg gaggcgatcc
     4321 ggcgctggct gatcgaccgc tacggcgcct gggtcagcta caggccgccg gtcgagccgc
     4381 tgacctggcc gctctgggcg gcgccgctgc ttcttttcgc cgccggcctc tacctggccc
     4441 gcggccgttt ccggcggaag ccgcgctgat gggctggctg atcctgctcc tgctggggct
     4501 cgccgccttt gccgccatct ggcgcttcgg gcggctcgac ggcgccggcg cccagctgct
     4561 cgccgccgcc ctcctgctcg gcttcgccgg ctatgcctgg cagggccgcc ccgggctggc
     4621 cggaagcccc aaggcgccgc ccgcgtctca ggccctgccg gacagcgcct tcgcccggat
     4681 gcgccaggac atgctcggcc ggttcgacac ggccgaccgc tggctgacga tcgccgaagg
     4741 ctatcagcgc cgcggcgaca cgcgcgccgg cgcaggcctg atccgggcgg cgctgcgcga
     4801 gcatcccgac aatgcgatcc tgtggatcgg ctacggcaat gcgctggtga tccacgccgg
     4861 cgggatgatc actccggccg cgcagctcgc cttcgagcgg gcgggccagc tcgcgcccga
     4921 ccacccggcg ccccgcttct tcttcggcgt ggcgctggcc cagtccggcc gcctcgacga
     4981 ggccgagcgg acctggcggg cgctgatcgc ggcggcgcct cccgacgccg cgtggcgcgc
     5041 cggagtcgag gcccagctcc agcaattgga gcaggcgagg gcagcgggca gataaatcct
     5101 ccccaccgtc cgagtgaagc aggatcgaga gataacggcg ccgaatcaga agcttctgaa
     5161 gaaaatctcc gaagccgtgc gagctaaccg accaatccct acgggtggtc cccctcccca
     5221 cgggctttgc ccgcagggag gatgagtcag tggtcgatgc cccgaccggt ctcgtgctgg
     5281 agctcgtcga tccgcttcga ggcttcggcc ttgctgagcc cgggatcgaa ttcctcgccc
     5341 gcttcctggc tcaaggtctt cagatatgag gcttgcgggc ccgtcatcga ttcgtcgccg
     5401 gtgacccatt cctcggggtc cttctccggg gtgggcggcg gagtctgctg agtcgacatg
     5461 atttttccct tgatgcaaca ccggctcaac caacggcgat gttccgatgt tgttccaggc
     5521 ggcctcaggc ggcctcgaac ggcaggtcga gcgcctcggc gaccgcccgg tgacggatct
     5581 tgccgcccga gacgttcagg ccgttggcca ggtgcggatc ggacttcatc gcgacctcag
     5641 caccttgccc ggcgagctta agaacgaacg gcaaggtcgc attgttgagc gcgaaggccg
     5701 aggtgcgcgc gaccgcgccc ggcatgttgg ccacgcaata atggatgacc ccgtcgatct
     5761 cgtagacggg atcgtcgtgg gtcgtcgccc gcgacgtctc gaagcagccg ccctggtcga
     5821 tggcgatgtc gaccagaacc gagccgcgct tcatcgtctt gagcatgtcg cgggtgacca
     5881 gcttgggcgc cgccgcgccc ggcaccagga ccgcgccgat caccagctcg cacttgctca
     5941 cggcatgggc gatcgccgcc cgcgacgagt aagcggtctt gatctggctg ccaaagaaca
     6001 tgtcgagctc ggccagccgc gcgttggaga tgtcgtagat ggtgacgtcg gcgcgcatcc
     6061 cgaccgccat ctgggccgcg ttcaccccgg cgactccgcc gccgaggatc gcgacccggg
     6121 cgggcgcgac gccgggaacg ccgccgagga gcacgccgcg gccgccctgc tccttctcga
     6181 gataatgggc gccgacctgg atcgacatcc ggcccgccac ctccgacatc ggcttgagca
     6241 gcggcagcga gccgtcggcg gcggtcaccg tctcataggc gatgcaggtc gcgccggact
     6301 tcatcagccc ttcggtctgg ggccggtccg gggcgagatg gaggtaggtg aagagcaggt
     6361 ggcgaggctc gagcagggcg atctcgcggg cctgcggctc cttcaccttc acgatcatgt
     6421 cggcctcgcg gaaggtggag gcggcgtcgg gggcgatgcg ggcgccggcg tcgacatagg
     6481 cctggtcgtc gaagtcgatc cccatcccgg ccttggtctc gacgatgacc tcatggccgg
     6541 cgccggtgag ctcggccacc gagcccgggg tgaggccgac gcgatattcg tgattcttga
     6601 tctccttggg cacgccgacg cgcatgttcg cagctcctcg cagcttttcg gtttggcgcg
     6661 cctatagcgg ggcgatcggg ctttgtcgcc gttgcggaaa cgcccgcctc catgctagac
     6721 gcccgcgcct tccccgatga ccgacgtccc tcccgccacc gatccgaccg cggactcgcc
     6781 gcccggcgcg cgcccgcgct cccaggccgg gctcgccctc ggcgcgatcg gcgtcgtctt
     6841 cggcgacatc ggcaccagcc cgatctatgc cttccgagag gc
//
LOCUS       DASVQZ010000071         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_881817, whole genome shotgun sequence.
ACCESSION   DASVQZ010000071 DASVQZ010000000
VERSION     DASVQZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881817"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF547_01950"
     CDS             complement(<1..272)
                     /locus_tag="VF547_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261615.1"
                     /translation="MKRPLLLVLSLLVATAAAEPSDSEEAKLRAAVERGTLLFELDRA
                     AWVTTDDMLERFGRRDMPIKGWVTERDGADGYRVTYYGDGPGGPVA"
     gene            complement(316..972)
                     /locus_tag="VF547_01955"
     CDS             complement(316..972)
                     /locus_tag="VF547_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8261616.1"
                     /translation="MIDLYTAATPNGWKVSILLEELALPYEVHVLDLGAGTQKEAWFT
                     AINPNGRIPAIVDRDEGDFAVFESGAIMVYLAEKTGRLMPSDPKGRSRVMQWLMFQMG
                     GVGPMMGQANVFTRYFPEELPSVIDRYRRESRRLFEVLDGRLARSEWLAGDYSIADIA
                     NWAWVQTHEWPGVPVDGLDHLQRWLEAVRARPAVQKGVTVPPRADPAKSVATARSILA
                     "
     gene            complement(1050..3533)
                     /locus_tag="VF547_01960"
     CDS             complement(1050..3533)
                     /locus_tag="VF547_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8261617.1"
                     /translation="MTPLRLALRDLRGGLAGLRLLAVCLFLGVLALAGVGSLSAAIVA
                     SLAERGQEILGGDVQLVVSQREATAQELAAFSREGRISRITRMRAMAARPDGEDSVLV
                     ELKGVDESWPMFGMFRASPGRRPKGAQVAIGPELADRLGVRTGDRIRIGAAELEVSGL
                     IAEEPDRLGQGFTLGSTALVDGTGLAATGLVQPGSLYTSAYRLKLPPGADPTKVAERL
                     KRGFGAAGFQVQERTNGAPGTRRFIERLGQFLTLVGLTALIVAGIGVGNGVASYLDSK
                     RGTIATLKLLGATSRTLFLSYLFQVGLVALAATAAGLAAGALVPAAVVAVAGDALPVP
                     PRLSVYWPPLLASAAYGLLIAAVFALVPIARARTVPAAALFRGDLEPLRRPGPAVLLI
                     VAAAACAIAGLAVGTAREPGFAAGFIGSALGLVLLLTALGWLVRRTASLLPRPRHPVF
                     RIALANLYRPGAQTGRLVVALGLGLTLFTGLAVIETNLAGQIRTAIPARAPSFFMLDI
                     PVERIGDFRSLARRAAPEGELVTIPSLRGSVVAVGGRRVADMAQIPPEAWFLRGDRGL
                     TYSATLPQGSRIVAGRWWPSDYRGPPLVSLDVEAARAVGLKVGDPITVSVLGREIEAR
                     IASLREIDWDTMGFNFVIVFAPGVLESAPHSFMATIAMPEAQERPFARAVSRAFPTVS
                     AIRVKEVVETVGTMLSQLAVAVRAAASVAILAGIAVLVGALAASRRARTYDSVVLKML
                     GATRPRILAAQAAEFAALALVVGLVALALGSAAGWWVVVRLFELDWAPDWLSVASTLA
                     LGAVVTLVLGLVGSLPALAARPARALRTL"
BASE COUNT          559 a         1287 c         1323 g          416 t
ORIGIN      
        1 caggcgaccg ggcccccggg gccgtcgccg taataggtga cccgatagcc gtcggcgccg
       61 tcccgctcgg tcacccaacc cttgatcggc atgtcccggc gcccgaatcg ctccagcatg
      121 tcgtcggtgg tgacccaggc ggcgcggtcg agctcgaaca gcagcgtgcc gcgctcgacg
      181 gcggcgcgca gcttcgcctc ctcggaatcg gacggctccg ccgccgccgt cgcgaccagc
      241 agggagagga cgagcagcag cgggcgcttc atcgccggag cctagcaaag gccggcgggc
      301 ccgtacaggc gaggctcagg ccaggatcga cctcgccgtc gccaccgact tcgccggatc
      361 ggcccgcggc gggacggtca cgcccttctg aaccgccggg cgcgcccgca ccgcctcgag
      421 ccagcgctgg agatgatcga ggccgtccac cggcacgccc ggccattcgt gggtctggac
      481 ccaggcccaa ttggcgatgt cggcgatcga atagtcgccg gccagccatt cgctgcgggc
      541 gagccggccg tcgagcacct cgaacagccg ccggctctcg cgccggtagc ggtcgatcac
      601 gctcggaagc tcctccggga agtagcgggt gaagacattg gcctggccca tcatcggccc
      661 gaccccgccc atctggaaca tcagccactg catgacccgg ctccggccct tcgggtcgga
      721 cggcatcagc cggcccgtct tctcggcgag atagaccatg atcgcgccgg attcgaagac
      781 ggcgaaatcg ccctcgtcgc ggtcgacgat ggcgggaatc cggccgttgg ggttgatcgc
      841 ggtgaaccag gcctccttct gcgtcccggc gccgaggtcg aggacatgga cctcgtaggg
      901 cagcgccagc tcctcgagca ggatcgagac cttccagccg ttgggcgtgg cggcggtgta
      961 gagatcgatc atggggcgct cccgatgagg ttgggctcgc ctcggatggt atcacatcca
     1021 ccgttcggtt tcagtttcac cggcgccgcc tacagcgtcc tcaacgcccg cgccggcctg
     1081 gccgccaagg ccggaagcga tcccaccagc cccaggacga gcgtcaccac cgcgcccagc
     1141 gccagcgtcg aggccaccga gagccagtcc ggcgcccagt cgagctcgaa caggcgcacc
     1201 acgacccacc agccggcggc gcttcccagc gcgagggcga cgaggccgac gaccagcgcg
     1261 agcgcggcga actcggcggc ctgggcggcg aggatgcggg gccgcgtggc gccgagcatc
     1321 ttcaggacca cgctgtcgta ggtgcgcgcg cggcggctgg cggcgagcgc tccgacgagg
     1381 accgcgatgc cggcgaggat cgccaccgag gcggcggcgc gcaccgccac cgcgagctgg
     1441 gagagcatgg tcccgaccgt ctccaccacc tccttgaccc ggatcgccga caccgtcggg
     1501 aaggcccgcg agacggctcg ggcgaagggc cgctcctgcg cctcgggcat ggcgatcgtc
     1561 gccatgaagc tgtgcggcgc gctctcgagc acgccggggg cgaagacgat gacgaagttg
     1621 aagcccatcg tgtcccagtc gatctcgcgc agcgaggcga tccgcgcctc gatctcgcgg
     1681 cccagcaccg aaaccgtgat cggatcgccg accttcaggc ccaccgcccg agccgcctcg
     1741 acgtcgaggg agaccaaggg cggcccgcga tagtcgctcg gccaccagcg accggcgacg
     1801 atccggctcc cctgcgggag ggtcgccgaa taggtgaggc cgcggtcgcc gcgaaggaac
     1861 caggcctcgg gcggaatctg agccatgtcg gcgacccgcc ggccgccgac ggcgacgacc
     1921 gatccgcgca gcgacgggat ggtgacgagc tcgccttccg gcgccgcccg gcgggcgagg
     1981 ctgcggaagt cgccgatccg ctcgaccggg atgtcgagca tgaagaagct cggcgcgcgg
     2041 gcggggatgg cggtgcggat ctggccggcg aggttggtct cgatcaccgc caggccggtg
     2101 aacagggtga gtccgaggcc gagcgcgacg acgagtcggc cggtctgggc gcccgggcgg
     2161 tacaggttgg cgagcgcgat ccggaatacc ggatgcctcg gcctcggcag cagcgatgcg
     2221 gtccgccgga ccagccagcc gagcgcggtg agcagcagca cgaggccgag cgccgacccg
     2281 atgaagccgg ccgcgaagcc gggctcgcgc gcggttccga cggcgaggcc ggcgatggcg
     2341 caggcggcgg cggcgacgat caggagcacc gccgggccgg ggcgtcgaag cggctccagg
     2401 tcccctcgga acagcgccgc cgccggaacg gtccgcgccc gggcgatcgg caccagggcg
     2461 aaaaccgcgg cgatgagcag gccgtaggcg gcgctggcga gcaggggcgg ccaatagacg
     2521 gagagccggg gcggcaccgg cagcgcgtcc cccgccaccg ccacgactgc cgccggcacg
     2581 agcgccccgg ccgcgagccc cgcggccgtg gcggcgagcg cgaccagccc gacctggaac
     2641 agataggaca ggaacagcgt gcggctggtg gcgccgagca gcttcagggt ggcgatcgtg
     2701 ccgcgcttgg aatcgagata ggaggcgacc ccgttgccca ctccgatgcc ggcgacgatc
     2761 aaggcggtga ggccgaccag ggtcaggaac tggccgagcc gctcgatgaa gcggcgggtg
     2821 cccggggcgc cgttggtgcg ctcctggacc tggaagccgg cggcgccaaa gccccgcttg
     2881 agccgctcgg ccaccttggt cggatcggct ccgggcggaa gcttcagccg gtaggcgctg
     2941 gtgtagaggc tgccgggctg gacgaggccg gtggccgcga gtccggttcc gtcgaccagc
     3001 gcggtcgagc cgagggtgaa gccctgcccg agccggtccg gctcctcggc gatcaggccg
     3061 ctcacctcga gctcggccgc gccgatccgg atccgatcgc cggtccggac tccgagccga
     3121 tcggcgagtt ccgggcctat cgcgacctgg gcgcccttcg gccggcggcc gggagacgcg
     3181 cggaacatgc cgaacatcgg ccaggactcg tccaccccct tgagctcgac cagaacgctg
     3241 tcctcgccgt ccggccgcgc cgccatcgcg cgcatccggg tgattcggct gatccggcct
     3301 tcgcgcgaga aggccgcgag ctcctgcgcc gtcgcctcgc gctgggagac gacgagctgc
     3361 acgtcgccgc ccaatatctc ctgcccgcgc tcggccagcg aggcgacgat cgcggcggac
     3421 agcgagccga ccccggcgag ggccagaacg ccgaggaaca ggcagacggc gagcaggcgc
     3481 agcccggcga gcccgccccg aaggtcgcga agcgcgaggc ggaggggggt cacctaaatc
     3541 ctccccatgc gcagcatggg gagggggacc aggcgaagcc tggtg
//
LOCUS       DASVQZ010000072         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_304720, whole genome shotgun sequence.
ACCESSION   DASVQZ010000072 DASVQZ010000000
VERSION     DASVQZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304720"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VF547_01965"
     CDS             complement(<1..161)
                     /locus_tag="VF547_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEX8261618.1"
                     /translation="MSTQSPAPAIPPSLFAYSIVYGGMVCIAGVLANKQVALGPLGVE
                     AGIFAFLLLV"
     gene            237..1229
                     /locus_tag="VF547_01970"
     CDS             237..1229
                     /locus_tag="VF547_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX8261619.1"
                     /translation="MTGTRLDVLAIGDAIVDVIATADDSFLEEEGLVKGSMQVLDAAG
                     ATRLYAKMRQARETSGGSAANTMAGVAAMGGRAGFVGQVADDQLGEIFAHDIRALGVE
                     FNTPPLSGGAPTGRCLILVTPDAQRTMNTFRGAAHELTTDALDAQQIREAAILYLEAY
                     LWRSAGPRAAMEEAMRIARAAGRKVAFTLSDIACIAPHRAEMMAMIDGGAIDLLFANE
                     NEICELADLPDRAAAVEAIQARVPLLVVTCGAEGALAVQGGRTVSVPIARIGTGVIDT
                     TGAGDLFAAGFLVGQARGRSLEASLRIGSIAAAEVISHFGARPEAGLKALVEKL"
     gene            1306..1875
                     /locus_tag="VF547_01975"
     CDS             1306..1875
                     /locus_tag="VF547_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX8261620.1"
                     /translation="MRRLAVYCGSSMGTDPAFTEAARALGEEMARRGIGLVYGGGHRG
                     LMGVVADSVLTAGGEAFGVIPQALVELEVAHTGLTELHTVTSMHERKAKMTELTDAFV
                     ALPGGIGTFDELFEAWTWNALGYHAKPFALLNVAGFWDGLIGFLDQATMHGFMSPARR
                     AQLIVGTDIGDVIDRLDAAIAAAEPGMVW"
     gene            1886..>2813
                     /locus_tag="VF547_01980"
     CDS             1886..>2813
                     /locus_tag="VF547_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX8261621.1"
                     /translation="MGSDVNRILMGLAGIAAILAIAVLLSSNRRAIRLRVVGAAFALQ
                     AGIAVLVLYTSWGKAGIEGLSRGVANLLGYAGKGTEFLFGRLASDPLGQNFAIQALPV
                     IIFFAALVAILYYLGIMQRVVRWVGGAIGWVTGISRVESLGAAANIFVGQSESPLVVR
                     PYLPSLPPAQIFTLMTVGMAGVAGTILAAYAALLGEQYLPYLLAAAFMSAPGGILMAK
                     IMMPDEPRTDELPLEDGTAPAEEKIDVAETFEDGEQPANIIMAAAQGAQTGVKLAVAV
                     GAMVLAFVALVALANGLLGGIGGWFGIENLSFQ"
BASE COUNT          422 a          965 c          989 g          437 t
ORIGIN      
        1 accagcagca ggaaggcgaa gatccccgcc tccaccccca gcgggccgag cgcgacctgc
       61 ttgttggcca gcacgccggc gatgcagacc atgccgccat agacgatcga ataggcgaac
      121 agcgagggag ggatggcggg cgctgggctc tgggtcgaca tgacggctgc gtaaccggtt
      181 tgcgccgccg caaaaagggc tttccgtcgg gcgcgcttgc ggccatcacg gcgcgcgtga
      241 ctggaacccg cctggacgtc ctcgccatcg gcgacgcgat cgtggacgtc atcgccaccg
      301 ccgacgacag cttcctcgag gaggaagggc tggtcaaagg gtcgatgcag gtgctcgacg
      361 cggccggcgc cacccggctc tacgccaaga tgcgccaggc gcgggagacc agcgggggat
      421 cggcggcgaa cacgatggcc ggcgtcgccg caatgggcgg gcgcgccggc ttcgtcggcc
      481 aggtcgccga cgaccaactc ggcgagatct tcgcgcacga catccgcgcg ctcggagtcg
      541 agttcaacac cccgccgctc agcggcggcg cacccaccgg gcgctgcctg atcctcgtca
      601 cgcccgacgc gcagcggacg atgaacacct tccgaggcgc cgcccacgag ctgacgaccg
      661 acgcgctcga cgcccaacag atacgcgagg ccgcgatcct ctatctcgaa gcctatctgt
      721 ggcgctcggc aggtccgcgg gcggcgatgg aggaggcgat gcggatcgcc cgcgcggccg
      781 gccgcaaggt cgccttcacc ctctccgaca tcgcctgcat cgcgccgcac cgcgccgaga
      841 tgatggcgat gatcgacggc ggcgcgatcg acctcctgtt cgccaacgag aacgagatat
      901 gcgagctcgc cgacctgccg gaccgggccg ctgcggtcga ggcgatccag gcccgggtcc
      961 cgttgctcgt ggtaacctgc ggcgcggagg gagcgctcgc cgtccagggg ggccgcaccg
     1021 tctcggtgcc gattgcccgg atcggcaccg gcgtgatcga taccaccggc gccggggatc
     1081 tgttcgcggc cggcttcctc gtcggccagg cgcgcggacg cagcctcgag gcctcgcttc
     1141 gcatcggctc gatcgccgcg gccgaggtga tctcgcattt cggcgcccgt ccggaagcgg
     1201 gcctcaaggc cttggtcgag aaattgtgac tcgggtgacg aaccatgatc cctctcccct
     1261 ggggcgaggg ggcaaatcgg cgcagcctgc atccgagtcc cggcgatgag gaggctcgcc
     1321 gtctattgcg gctccagcat gggcaccgat cccgccttca ccgaggccgc gcgggcgctg
     1381 ggcgaggaga tggcgcggcg gggcatcggg ctcgtctatg gcggcggcca tagagggttg
     1441 atgggggtgg tcgcggactc cgtgctcacg gcgggcggcg aggccttcgg agtgatcccc
     1501 caggcgctgg tcgagctgga ggtcgcccac accgggctca ccgagctcca tacggtcacg
     1561 tcgatgcacg agcgcaaggc caagatgacg gagctgaccg acgccttcgt cgccttgccg
     1621 ggcggaatcg ggacgttcga cgaattgttc gaagcctgga cgtggaacgc gctgggctat
     1681 cacgccaagc ccttcgccct gctcaacgtc gccggcttct gggacgggtt gatcggcttc
     1741 ctcgatcagg ccacgatgca cggattcatg tcgcccgccc ggcgggccca gctgatcgtc
     1801 ggcaccgaca tcggcgatgt gatcgaccgt ctcgacgccg ccatcgcggc ggcggagccg
     1861 ggaatggttt ggtgaggggg caggcatggg cagtgacgtc aatcggatcc tgatgggtct
     1921 cgcgggtatc gcggcgatcc tggcgatcgc cgttctgctc tcctccaacc gccgcgccat
     1981 ccggcttcga gtcgtcggag ccgccttcgc actccaggcc ggcatcgccg tgctcgtgct
     2041 ctacaccagc tggggcaagg ccgggatcga agggctttcg cgcggagtgg ccaacctgct
     2101 cggctatgcc ggcaagggca cggaattcct gttcggccgc ctcgccagcg acccgctcgg
     2161 ccagaatttc gcgatccagg ccctgccggt gatcatcttc ttcgcggctc tcgtcgccat
     2221 cctctattat ctcggcatca tgcagcgggt ggtccgctgg gtcggcggcg cgatcggctg
     2281 ggtgaccggc atcagccgag tcgaatcgct cggcgccgcc gccaacatct tcgtcggcca
     2341 gagcgagtcg ccgctggtgg tgcggccgta tctgccgagc ctgccgccgg cgcagatctt
     2401 caccctgatg acggtcggca tggccggcgt ggcgggcacc atcctcgccg cctacgccgc
     2461 tttgctcggc gagcaatatc ttccctatct gctggcggcg gcgttcatgt cggcgccggg
     2521 cggcatcctg atggccaaga tcatgatgcc ggacgagccg cgaaccgacg agctcccgct
     2581 ggaggacggc accgcgccgg cggaggagaa gatcgacgtg gccgagacgt tcgaggacgg
     2641 cgagcagccg gccaacatca tcatggccgc cgcccagggg gcgcagaccg gcgtcaagct
     2701 cgccgtcgcg gtcggcgcga tggtgctcgc cttcgtggct ctggtggccc tcgccaacgg
     2761 gctgctcggc ggcatcggcg gctggttcgg gatcgagaat ctgagcttcc agc
//
LOCUS       DASVQZ010000073         4593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_304792, whole genome shotgun sequence.
ACCESSION   DASVQZ010000073 DASVQZ010000000
VERSION     DASVQZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304792"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VF547_01985"
     CDS             <1..1005
                     /locus_tag="VF547_01985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8261622.1"
                     /translation="GDSALGFEVELFDSLEAAAADAGGALDRSASPFDRLAWFRLVEA
                     HCPPPGRPLVARARRGDGAAAWLFLARQGRRAEAWRCWYSLRFGLIGAEEGAEPIARI
                     LRKRLSRLEIAPVAGPRALALAFRRAGWIVFLEHATISWQAETAGKDFAAYWAARPGK
                     LRSTAQRKAKAAGLEIELHRVFDPAAWSAYEEVYAASWKPEEGAPAFLRAFAEQEGEA
                     GRLRLGIARKDGRPLAAQLWTVDGGTAWIHKLAYREDSKSLSPGTVLSMAMFRAALDE
                     DRVERIDYGTGDDAYKRDWMDERRILWRLEAFNPMSPAGLAGAARAGLSALVRRARSR
                     "
     gene            1034..1312
                     /locus_tag="VF547_01990"
     CDS             1034..1312
                     /locus_tag="VF547_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8261623.1"
                     /translation="MPTAYDDEQEVDATLRAVLRDVLGLPQAQVAAMDESSPLFGALP
                     EFDSMAVAGLLTELEERLGILIEDHEVDADMLESFGSLLTFARAKSLQ"
     gene            1309..1956
                     /locus_tag="VF547_01995"
     CDS             1309..1956
                     /locus_tag="VF547_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261624.1"
                     /translation="MTGPGLDYARYRVGGRDEWMMRIGCGPALLFLPPLFEEMNRTRA
                     LLAAAMRALAADGFACWLPDLPGTGESGRSLEEVSWQDWSDAAAAALEASGAAASVSL
                     RGGALLDEAAPARWRFAPATGASLARDLARAGLMTEGGGGYAPSAALLAALAEAEPAT
                     GGTLRTLRLATDRAEADLKLEGPPLWRRAEPQNSYELATAIASDISQWARACGAC"
     gene            1941..2735
                     /locus_tag="VF547_02000"
     CDS             1941..2735
                     /locus_tag="VF547_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HEX8261625.1"
                     /translation="MRRLLSFDCEGASLGASLDSADGETGLLLVTGGTQTRIGSHRMY
                     ERLAAALAREGWPCFRYDRRGVGDSEGEDPDFKGSGPDLAAAASAFRREQPQLNRLLG
                     FGLCDGASTLALHGKAAGLDGYVLVNPWFVEAEAGEPPAAAIKSRYKDQLLSLDGWKR
                     LLSGSISYRKVLKGLGRIIASRPSSLSGEIAASLAEARVPAQLVLATRDPTAIAARAE
                     WSSPPFKRLREANPAPLTIDSDAHTFSRPGDEAALLEAVKVALARL"
     gene            complement(2759..3736)
                     /gene="trxB"
                     /locus_tag="VF547_02005"
     CDS             complement(2759..3736)
                     /gene="trxB"
                     /locus_tag="VF547_02005"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX8261626.1"
                     /translation="MPATQSTRMLILGSGPAGLSAAIYGARAGLAPIVVQGIQPGGQL
                     TTTTDVENYPGFRDVIQGPWLMEEMQAQAEHVGARMVWDHIAEVDLSSRPFRLIGDAG
                     TVYSGDVLVIATGAQARWLGLPSEEHLKGRGVSACATCDGFFYRGKKVAVIGGGNTAV
                     EEALYMTNHSHDVTLIHRRDSLRAEKILQDRLFANPKIKVLWNKAVDAFVAGGEPEGL
                     VALDLRDTRTGETSRLDVEGGFVAIGHTPATELFRDKLVLDKDGYIEVEPGSTRTSVE
                     GVFACGDVMDKIYRQAVTAAGTGCMAALDAEKFLAEAEFEASAAVHEVA"
     gene            3831..>4593
                     /locus_tag="VF547_02010"
     CDS             3831..>4593
                     /locus_tag="VF547_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8261627.1"
                     /translation="MPHDNPLGLDGFEFVEFTSPDPEALGDLFRRLGFAHRGDHKTKR
                     IRHYAQGNINFLLNMEASGHAENFRGAHGPSASAMAFRVADAERALGLAVERGATAVE
                     GEYGLPAIDGIGGSYLYLVDRYGAETIYDRDFTPVGAEGDRAGAGLHTLDHLTHNVHR
                     GRMDHWAQFYERIFNFRQIRYFDIEGQQTALLSRAMTAPDDKIRIPLNESQDEFSQIE
                     EFLKEYKGEGIQHLALATDDIFATVDALRANGIRFQ"
BASE COUNT          663 a         1636 c         1614 g          680 t
ORIGIN      
        1 ggggatagcg cgttgggctt cgaggtggaa ctgttcgata gcctcgaggc ggccgccgcc
       61 gacgccggcg gcgcgctcga ccggagcgcc tcgccgttcg accggctcgc ctggttccgc
      121 ctggtcgagg cccattgccc gccccccggc cggccgctgg tcgcccgcgc ccgccgcggc
      181 gacggcgcgg cggcctggct gttcctcgcc cggcagggcc ggcgggcgga ggcgtggcgc
      241 tgctggtaca gcctcaggtt cggcctgatc ggcgccgagg agggcgccga gccgatcgcc
      301 aggattctgc gcaagcgcct gagccggctg gagatcgcgc ctgtcgccgg cccgcgcgcc
      361 ctcgccctgg cgttccggag ggccggctgg atcgtcttcc tggagcacgc caccatctcc
      421 tggcaggcgg aaacggccgg caaggatttc gcggcctatt gggcggcgcg ccccggcaag
      481 ctgcgcagca ccgcccagcg caaggccaag gccgcgggac tcgagatcga gctccaccgc
      541 gtcttcgatc ccgctgcctg gtcggcctat gaggaggtct acgccgcgag ctggaagccc
      601 gaggaaggcg cccccgcctt cctgcgcgcc ttcgccgagc aggagggcga ggccggacgg
      661 ctccggctgg gcatcgcccg caaggacggc cggccgctgg cggcgcagct ctggacggtg
      721 gacggcggca ccgcctggat ccacaagctc gcctatcgcg aggattcgaa gtcgctctcg
      781 cccggcacgg tgctgagcat ggcgatgttc cgcgccgcgc tggacgagga ccgggtcgag
      841 cggatcgact atggcacggg cgacgatgcc tacaagcgcg actggatgga cgagcgccgc
      901 atcctgtggc ggctggaggc cttcaacccg atgtcgcccg ccggcctggc cggagcggcc
      961 cgggccgggc tatcggcgct tgtccgccgc gctcgaagcc gttaggcgcg gcgcgccacg
     1021 aggggagccc cgaatgccga ccgcctatga cgacgaacag gaagtcgacg cgacgcttcg
     1081 ggcggtgctg cgcgacgtgc tcggactgcc gcaggcccag gtggcggcga tggacgagtc
     1141 gagccccttg ttcggagccc tgccggaatt cgactcgatg gcggtcgccg ggcttctgac
     1201 cgagctcgag gaacggctcg ggatcctcat cgaggaccat gaggtcgacg cggacatgct
     1261 tgagagcttt gggtcccttc tcaccttcgc gcgggccaaa tcgctccagt gacgggcccc
     1321 ggcctcgatt atgcccgcta ccgggtggga ggccgcgacg aatggatgat gcggatcggc
     1381 tgcgggcccg cgctgctgtt cctgccgccg ctgttcgagg agatgaaccg gacccgggcc
     1441 ctgctcgccg cggcgatgcg cgcccttgcc gcggacggct tcgcctgctg gctccccgac
     1501 cttcccggca cgggcgagag cggacgctca cttgaggagg tctcgtggca ggactggagc
     1561 gacgccgccg ccgccgcgct cgaggcttcc ggggccgccg cgtcggtcag cctgcgcggc
     1621 ggcgccctgc tcgacgaagc ggctccggcg cgctggcgct tcgcgccggc caccggcgcc
     1681 tcgctggcgc gcgatctcgc ccgggccggg ctgatgacgg agggcggcgg cggctatgcg
     1741 ccctcggccg ccctgctcgc cgccctcgcc gaggcggagc cggcgacggg cgggacgctt
     1801 cggaccttgc gcctggcgac cgaccgggcc gaggccgatc tgaagctcga aggaccgccg
     1861 ctctggcgcc gggcggaacc tcagaattcc tatgagctcg ccacggcaat agcttccgac
     1921 atatcgcaat gggcacgcgc atgcggcgcc tgctgagctt cgactgcgag ggcgcgtcgc
     1981 tcggcgccag cctcgattcc gccgacggcg agacgggcct cctgctcgtc accggcggca
     2041 cccagacccg gatcggatcg caccggatgt acgagcggct ggcggccgcc ctggcccggg
     2101 agggctggcc ctgcttccgc tacgatcgcc gcggagtcgg cgacagcgag ggcgaggatc
     2161 cggacttcaa ggggagcggc cccgatctcg ccgcggccgc ctccgccttc cgccgcgagc
     2221 agccgcagct caatcgcctg ctcggcttcg gcctgtgcga cggcgcctcg acgcttgcgc
     2281 tccacggcaa ggcggccggg ctcgatggct atgtcctcgt caatccctgg ttcgtcgagg
     2341 ccgaggccgg agagccgccg gccgcggcga tcaagtcgcg ctacaaggac cagcttctca
     2401 gcctggacgg ttggaagagg cttctttccg gatcaatatc ttaccgcaag gtcctgaagg
     2461 gattgggaag gataatcgcg agccggccgt cgagcctgtc gggcgagatc gccgcctcgc
     2521 tcgcggaggc cagggttccc gcgcagctgg tcctcgcgac ccgcgacccg acggcgatcg
     2581 ccgctcgggc ggaatggtcg tcgcccccct tcaagcgcct gcgcgaggcc aatccggcgc
     2641 cgctgacgat cgacagcgat gcgcacacct tctcccgccc gggggacgag gcggccttgc
     2701 tcgaggccgt gaaggttgcg ctggcgcggc tgtaacccct ccattccagg gaagggaatc
     2761 aggccacctc gtgaaccgcc gccgaagctt cgaactcggc ctcggccagg aacttctcgg
     2821 cgtccaaggc cgccatgcag cccgtcccgg ccgccgtcac cgcctggcgg tagatcttgt
     2881 ccatgacgtc gccgcaggcg aagacgccct cgacgctggt ccgggtgctg cccggctcga
     2941 cctcgatata gccgtccttg tcgaggacca gcttgtccct gaacaattcg gtggcgggcg
     3001 tgtggccgat ggcgacgaac ccgccctcga cgtcgagccg cgaggtctcg ccggtgcggg
     3061 tgtcgcgaag gtcgagggcg accagcccct ccggctcgcc gccggcgacg aaggcgtcga
     3121 ccgccttgtt ccaaagcacc ttgatcttcg ggttggcgaa cagccggtcc tgcaggatct
     3181 tctcggcccg aagcgagtcg cggcggtgga tcagggtgac gtcgtggctg tggttggtca
     3241 tgtagagcgc ttcctcgacg gcggtgttgc cgccgccgat caccgccacc ttcttgccgc
     3301 ggtagaagaa gccgtcgcag gtcgcgcagg ccgagacgcc cctccccttc agatgctctt
     3361 cggaaggcag gcccagccag cgcgcctggg cgccggtggc gatgaccagg acgtcgccgc
     3421 tgtagacggt gccggcgtcg ccgatcagcc ggaacggacg gctcgacaga tcgacctcgg
     3481 cgatatggtc ccagaccatc cgggcgccga catgctcggc ctgggcctgc atctcctcca
     3541 tcagccacgg gccctggatg acgtctcgga agcccggata attctcgacg tcggtggtgg
     3601 tggtcagctg gccgcccggc tggatgccct ggacgacgat cggagccagc ccggcgcgcg
     3661 cgccgtagat ggccgcggag aggccggccg ggcctgagcc gaggatgagc attcgggtgg
     3721 attgggtcgc gggcatgacg gcgagatagg agcttggggt ggaggcgcgc aacccatttg
     3781 ccgcttcccc ggcgccttga tagagagccg ccagcgcgag gagtaagacg atgccccacg
     3841 acaatccgct aggcctcgac ggcttcgagt tcgtcgagtt caccagcccg gatcccgagg
     3901 cgctcggcga cctcttccgc cgcctcggct tcgcccaccg cggcgaccac aagaccaagc
     3961 ggatccgcca ctatgcgcag ggtaacatca acttcctgct caacatggaa gcgtccggcc
     4021 acgccgagaa tttccgcggc gcccacggcc cgtcggccag cgccatggcc tttcgggtcg
     4081 ccgacgccga gcgcgctctg gggctggccg tcgagcgggg cgcgacggcg gtcgagggcg
     4141 agtacggcct gcccgcgatc gacgggatcg gcggctccta cctctatctc gtcgaccgct
     4201 acggcgccga gaccatctac gaccgggatt tcacccccgt cggcgcggag ggcgatcgtg
     4261 ccggagccgg gctccacacg ctcgaccacc tcacccacaa cgtccaccgc ggccggatgg
     4321 accattgggc gcaattctac gagcgcatct tcaacttccg ccagatccgc tatttcgaca
     4381 tcgagggcca gcagaccgcc ctcctgtcgc gggcgatgac cgcgcccgac gacaagatcc
     4441 gaatcccgct caacgagagc caggacgagt tcagccagat cgaggagttc ctgaaggaat
     4501 ataagggcga gggcatccag catctggcgc tggccaccga cgacatcttc gccaccgtgg
     4561 acgcccttcg cgccaacggc atccgcttcc agg
//
LOCUS       DASVQZ010000074         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_16244, whole genome shotgun sequence.
ACCESSION   DASVQZ010000074 DASVQZ010000000
VERSION     DASVQZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16244"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1116)
                     /locus_tag="VF547_02015"
     CDS             complement(100..1116)
                     /locus_tag="VF547_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8261628.1"
                     /translation="MYEQVYGFTERPFQLTPDARYWFESRTHKKAMAYLGYGLAQGEG
                     FIVVTGEIGAGKSTLVAHLMATIDRERLNAISLVSTQVEGDDMLRLAAQGLGLETGGV
                     MKAQLLDRIEQRLREEASRGKRTLLVVDEAQNLPVSALEELRMLSNFTSQGRALIQIF
                     LLGQPEFRDRVADDDSLEQLRQRIIAAHHLEPIGANEVEPYVVHRLAVAGWQGRPAFE
                     PDAFAALYRHAGGVPRKLNQLASRLLLYAGVEQLDSIGGGDVDSVAADMAADRPASAS
                     SRVLPLRRPAAACEPAGDSSVERRIAAIEARLDEQEAAVRRVLTLLLDWVENESRPRR
                     SNAA"
     gene            complement(1132..2772)
                     /locus_tag="VF547_02020"
     CDS             complement(1132..2772)
                     /locus_tag="VF547_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261629.1"
                     /translation="MKKALFQGPPSRRWLSVAATIGVSLIAGTAAVQGEPRTSVHPYL
                     EVQQVATMDFDRGEVLTYTGVGGGIDASVATRRVQATISYNYQRRIGWNDRLEDHDVH
                     SGLAAVHVDAVPGLLAFDAGAMAARTHADIRFPVPAARTIDSSAVAEVYSAYAGPTLT
                     SHVGPVSVGASYRLGYVKVDDHSLAGTGLPPGTPRVDRYSSSTVHNANVSFGMAPGRL
                     PFGWTVGAGYVREDMNRLDSKFEGHYVRGDVVLPVSPTLAVTGGVGYENMQGSQQDFL
                     RDPATGLPVLTPGGNLIPDKSRPRLETYDHDGYMWDVGLIWRPTRRTELQARGGWRYG
                     GESFTASLSHKINAHSALNASIYDSVSSFGRLLVTDLSGLPTKFQAPSNGSLTGAGCV
                     FGNDPGTGACFGDAFQSISNFNFRNRGATIRYSAARGPWSMGLGAGYANRKYLAPDSS
                     IFALRGVTDQSFNLQGNLGRRLTRSSGIDLDAYAAWYDSGLAGSDSSFGAGLTGRYYR
                     NIFRDRLQAQIAAGLYTTQAGELDASYGSILFGLRYTF"
     gene            complement(2769..3806)
                     /locus_tag="VF547_02025"
     CDS             complement(2769..3806)
                     /locus_tag="VF547_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261630.1"
                     /translation="MNKHSPLSQSGSLLERAAELYGFGPALAADPAPRRQPGAAEELG
                     PGLRRGAAGGEEDGRGEEADCAPRRGAGPGAADELAPGLRRDEEAAGQARSLLPVVTE
                     AKAPVDRDKLHERGFVVPDSPAGPLAEEFRIVKRQLLLGMGAGSPVAADKRRTVLVCS
                     AQPDEGKTFCAVNLALSLAGERDLEVLLVDADFPKPEVLDLLGIEAKGAGFVDALADP
                     SLDPERLVIRTDVGGLSVLGPGRYENDVPELLGSERTAQVLARLTEARPKRIVIFDSP
                     PALMASAAAALASHVGQLLLVVRADQTTEADLKEAAGLLSGCPQISLLLNGTGFAATG
                     RRFGSYYGYGQ"
BASE COUNT          579 a         1368 c         1453 g          583 t
ORIGIN      
        1 gaagcatggt ggaggggttc ttccggagtc ggcaaggctc ctccaccatg cttcgcacgg
       61 tccccctccc cgcgaaatcg cggggaggaa tcatggatct caagcggcat tgctgcggcg
      121 cggccggctt tcgttctcga cccagtcgag cagcagggtc aagacccggc gaacggccgc
      181 ttcctgctcg tccagccggg cctcgatcgc cgcgatccgg cgctcgaccg aggagtcgcc
      241 ggccggctcg caagccgccg ccggccggcg cagcggaagc acccgactgg aggcgctggc
      301 cggccggtcg gcggccatgt cggccgcgac tgaatcgacg tcgccgccgc cgatcgagtc
      361 gagctgctcg acgccggcgt agaggagcag gcgcgaggcg agctggttca acttgcgcgg
      421 aacgccgccg gcgtggcggt agagcgcggc gaaggcgtcc ggctcgaacg cgggacgccc
      481 ctgccagccc gccaccgcca gccggtggac gacatagggc tcgacctcgt tcgcgccgat
      541 cggctcgaga tgatgggcgg cgatgatccg ctggcgcagc tgctcgaggc tgtcgtcgtc
      601 ggcgacccgg tcccggaact ccggctgccc gagcaggaag atctgaatga gcgcacggcc
      661 ttgcgaggtg aagttggaga gcattcgaag ctcttcgagg gccgagacgg gcaggttctg
      721 ggcctcgtcg acgaccagca gcgtgcgctt gcccctcgac gcctcctcgc gcagcctctg
      781 ctcgatccgg tcgagcagct gcgccttcat gacgcccccg gtctcgagcc cgagcccctg
      841 ggcggcgagg cggagcatgt cgtcgccctc gacctgggtc gagacgaggc tgatcgcgtt
      901 cagccgctcg cggtcgatcg tcgccatcag atgggcgacc agggtcgact tgccggcgcc
      961 gatctcgccg gtgacgacga tgaagccctc gccctgggcg aggccgtagc cgagataggc
     1021 catcgccttc ttgtgggtac ggctctcgaa ccagtagcgc gcgtccggcg tcagctggaa
     1081 gggccgctcg gtgaagccgt agacttgctc gtacatgtcc ttgacgtccc tctagaaggt
     1141 gtagcgcagg ccgaacagga tcgagccgta gctggcgtcg agctccccgg cctgggtcgt
     1201 gtagaggccc gccgcgatct gcgcctgcag ccgatcgcgg aagatgttgc ggtagtagcg
     1261 gccggtgagg cccgcgccga agctcgaatc ggagccggcg aggccgctgt cgtaccaggc
     1321 cgcataggcg tcgaggtcga tgcccgacga gcgcgtcaga cggcggccga gattgccctg
     1381 caggttgaag ctctggtcgg tcactccgcg aagggcgaag atgctgctgt cgggagccag
     1441 atacttgcgg ttggcgtagc cggcgccgag gcccatgctc cacggcccgc gcgccgccga
     1501 gtagcggatg gtcgcgccgc ggttgcggaa gttgaagttg ctgatcgact ggaaggcgtc
     1561 gccgaagcag gcgccggtgc ccggatcgtt gccgaacacg cagcccgcgc cggtcaggct
     1621 gccgttcgac ggagcctgaa acttcgtcgg caagccactg agatcggtca ctaaaagccg
     1681 gccgaagctg cttacgctgt cgtagatgga ggcgttcagc gccgaatggg cgttgatctt
     1741 gtgcgacagc gaggccgtga agctctcgcc gccgtagcgc cagccgcccc gcgcctgcag
     1801 ctcggtccgg cgggtcggcc gccagatcag gcccacgtcc cacatgtagc cgtcatggtc
     1861 gtaggtctcg agccgcggcc gggacttgtc ggggatgagg ttgccgccgg gggtgagcac
     1921 cggcaggccg gtcgccgggt cgcgcaggaa atcctgctgc gagccctgca tgttctcata
     1981 gcccaccccg ccggtgacgg cgagcgttgg cgacaccggc aggacgacgt cgccccgcac
     2041 ataatggccc tcgaacttgg aatcgagccg gttcatgtcc tcgcggacgt agccggcgcc
     2101 gaccgtccag ccgaacggca gccggccggg cgccattccg aagctgacat tggcgttgtg
     2161 gacggtgctc gacgaatagc ggtcgacccg cggagtgccc ggcgggaggc cggttccggc
     2221 gaggctgtgg tcgtcgacct tgacgtagcc gagccggtag ctggcgccga ccgagacggg
     2281 cccgacgtgg ctggtcaggg tcggcccggc ataggcgctg tagacctcgg cgacggcgct
     2341 gctgtcgatc gttcgcgccg ccggcaccgg gaagcggatg tcggcatggg tgcgcgccgc
     2401 catggcgccg gcgtcgaagg cgagcaggcc cgggaccgcg tcgacatgga cggcggcgag
     2461 gccgctgtgg acgtcatggt cctcgagccg gtcgttccag ccgatccggc gctggtaatt
     2521 gtagctgatc gtcgcctgga cccgcctcgt cgcgaccgag gcgtcgatgc cgccgccgac
     2581 tccggtgtag gtcagcacct cgccgcggtc gaaatccatc gtcgcgacct gctgcacctc
     2641 cagatagggg tggacgctgg tcctcggctc gccctggacg gcggccgtcc cggcgatcag
     2701 gctgaccccg atcgtcgctg cgacggagag ccagcgacgg ctcggaggtc cttggaaaag
     2761 agcctttttc attggccata tccataatag gaaccgaatc ggcggccggt cgccgcaaag
     2821 cccgtgccgt tgagcagcag gctgatctgc gggcagccgg agaggaggcc cgcggcctcc
     2881 ttcaggtccg cttcggtggt ttgatcggca cgcacgacaa gcagcagctg accgacgtgg
     2941 ctcgccaggg ccgcggccgc ggaggccatc agggccggcg gcgaatcgaa gatcacgatc
     3001 cgcttcggcc gcgcctcggt gagccgggcg aggacctggg cggtgcgctc ggacccgagc
     3061 agctcgggca cgtcgttctc gtagcgcccg ggcccgagca ccgagaggcc gccgacgtcg
     3121 gtccggatca ccaggcgctc gggatcgagc gagggatcgg cgagcgcgtc gacgaagccg
     3181 gcgcccttcg cctcgatgcc gagcaggtcg agcacctcgg gcttggggaa gtcggcgtcg
     3241 accaggagca cctcgaggtc gcgctcgccg gccagcgaca gcgccaggtt gacggcgcag
     3301 aaggtcttgc cctcgtcggg ctgggccgag cagaccagga ccgtccgccg cttgtcggcg
     3361 gccaccgggc tgccggcgcc catcccgagc agaagctggc gcttgacgat ccggaactcc
     3421 tcggcgagcg ggcccgccgg gctgtcggga acgacgaagc cgcgctcgtg cagcttgtcg
     3481 cggtccaccg gcgccttggc ttcggtgacg accggaagca agctgcgagc ctgcccggcg
     3541 gcctcttcat cccggcgaag gccgggggcc agctcgtccg cggcgccggg ccccgccccg
     3601 cgccggggag cgcaatccgc ttcctccccg cgtccgtctt cctcgccgcc ggctgcgccc
     3661 cggcgaaggc cggggcccag ctcttccgcg gctccgggct gccggcgggg agcgggatcc
     3721 gcggcgagcg ccgggccgaa gccgtagagc tccgccgccc gctcgaggag cgagccgctt
     3781 tggctgaggg ggctgtgctt gttcatggtc cgggctccct cacgccaccg tgcttcgctg
     3841 ccagaactcg accgcgagca gcagcgccca ggcggcgccg agcgcgcccg cgccgccgcc
     3901 gagccagacc agccgctgcc ggtgcctcgc ccgggtctcc tcggtgaaca cctcggagat
     3961 cgagccgagc acgggcaggc cgg
//
LOCUS       DASVQZ010000075        18216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_785362, whole genome shotgun sequence.
ACCESSION   DASVQZ010000075 DASVQZ010000000
VERSION     DASVQZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18216
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785362"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VF547_02030"
     CDS             complement(<1..174)
                     /locus_tag="VF547_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX8261631.1"
                     /translation="MTLDTISVARSHGGTQHVVKHASRATGTDMTFSVFVPPQADEGA
                     ACPVVWYLSGLTCT"
     gene            complement(274..1386)
                     /locus_tag="VF547_02035"
     CDS             complement(274..1386)
                     /locus_tag="VF547_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX8261632.1"
                     /translation="MKTRAAVAVAPNRPLEIHEVDLDGPKAGEVLVEIMATGICHTDA
                     YTLEGKDSEGTFPSILGHEGAGVVREVGEGVTAVRPGDHVIPLYTPECRQCRSCLSRK
                     TNLCTAIRATQGKGVMPDGTSRFRLGGETIHHYMGCSTFSNFTVMPEIAVAKVREDAP
                     FDRICYVGCGVTTGVGAVIWTAGVEPGSTVVVFGLGGIGLNVIQGARLVGAARIVGVD
                     INPAKRAMAEAFGMTDFVNPNEVGADRLVQALVDLTGGGADYSFDCTGNTDVMRAALE
                     CCHRGWGVSTIIGVAEAGREIATRPFQLVTGRVWKGSAFGGARGRTDVPRIVDWYMDG
                     RIAIDPLITHTMPLDDINKAFDLMHAGESIRSVVIY"
     gene            1575..2087
                     /locus_tag="VF547_02040"
     CDS             1575..2087
                     /locus_tag="VF547_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX8261633.1"
                     /translation="MLIALAAAAAFQSSAPPPPTPPCSTPEYRQLDFWVGEWDAEFAL
                     PGGKTGRATNRITRDEFGDCVIAEHFSQPDTGYLGASFSTWDRLKKKWVQTWVDNGGA
                     YITLAGGPVEGQPFSFELVTLDPRGPQQVHSRMIWQDVKPNSFTWRWQARQPDGSWAD
                     SWVIKYTRRK"
     gene            complement(2206..3789)
                     /locus_tag="VF547_02045"
     CDS             complement(2206..3789)
                     /locus_tag="VF547_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8261634.1"
                     /translation="MTPDTDVLVIGAGHNGLVCAYYLAAKGLKVTILERRGVVGGAAV
                     TEEFHPGFRNSTASYTVSLLNPKVIEDMRLYEHGLKVVLRRIDNFLPTLGPDHLLSGA
                     GGLTRREIARHSPADADGYDAYSAALRSVVAILRDWILRAPPNAGGGLADVLAALKLG
                     NAVRGMSAEEQRNLLDFFTKSAADILGRYFETDIVQALFGFDAVVGNYASPYDPGSAY
                     VLLHHLFGEAAGVAGAWGHAIGGMGAITQAMAKAARERGVEIVLDSPVEEIIVERGRA
                     AGAVAGGRAWRAKAVAANVNPRFLFGSLLPDGAVAAEISRRMAGWAAESATFRMNVAL
                     SELPRFASLTEPGDHLTAGIIIAPSLRYMDRAYTSARTEGWAREPIVEMLIPSTLDDS
                     LAPPGGHVASLFCQHFPYEVEGGWEARVEEAADHVIDHVDRYAPGFKASVVGRLALSP
                     WHLEQRFGVPRGDIFHGKMGLDQLFSARPMLGHADYRMPLPGLYLCGSGAHPGGGVTG
                     APGHNAAAAIWADRRKWKR"
     gene            3885..4436
                     /locus_tag="VF547_02050"
     CDS             3885..4436
                     /locus_tag="VF547_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261635.1"
                     /translation="MKILVYLASPITISLILIGAMRIDGEFSRDIPQPPAVVAAALET
                     VDVVDQPHAQKALREAGEKRVPQILAQRDPDGFSWFVMSRGKSVLRMKAQLVPSADGA
                     STHVSTEVEPGEVEAGPDVPPLFASRSEVEPLFAIAVERALGDYIPRSERSLASLQEL
                     PARYKTQEEMNAEYEARHPDSSF"
     gene            4479..5264
                     /locus_tag="VF547_02055"
     CDS             4479..5264
                     /locus_tag="VF547_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261636.1"
                     /translation="MLKMLLKNFGFFILFVTGTAYVSGGLAGGAYSREVERPPAEVMA
                     ALADLDITAQPGSPGTDPSRSGGVQPVFRLERSGDRMTWTVMSGEQVAVRMIALVEPV
                     RDGKHSRVTASVERGDAPDDVVSPAFRSEGITMGLFGMALEGELNRLTAPPPADQARC
                     DALMAEFEAGNMAAGFAERPDNLTEAVGNTAKIAVRLHAMEAELRRNGCPTEPSRDFV
                     EVSDEMSNGGQGGQAPIADIPVEGISFEPGKPMVDLSQGKRRN"
     gene            complement(5304..5768)
                     /locus_tag="VF547_02060"
     CDS             complement(5304..5768)
                     /locus_tag="VF547_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8261637.1"
                     /translation="MSGLDGLDRKLLACLQQDNLMTAEALADRVGRSPSAVARRLRRL
                     RDSGAVVADVALVSEQAAGHPLSAVIHVQLERHALTEVASFKRQLAASQNVQFCLEIS
                     GAFDILLLVVVADMEAFNLFADDLLAGQRAVRRYETSFVKRRLKASLALPLD"
     gene            5883..7223
                     /locus_tag="VF547_02065"
     CDS             5883..7223
                     /locus_tag="VF547_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8261638.1"
                     /translation="MNRHGIALGIAAAALAGAAGAPAQPARTGPAPAASPAPAAGEAV
                     VAELRRVLAANYVLPEVRPKLDSALGRGLAAGRYATTDPAVLVERVNADLAAVTKDKH
                     LGLHYDPRQAAEIAAAPQGQAEDGPPTPGQIRQARLRNHGFVEMKVLPGNVRYVDLQG
                     FVWVGPESAAAYDNAMRFLAGGDAAIVDLRKNGGGSPEAVQYLVSHFMEPNRPLVTFH
                     MGATQVDRLATLAELPAGRMVGKPLYVLTSGHTASAAEEFAGHVAGYRLGELIGETTA
                     GAGFRNSFFALPGGYVVSVSVGRAVLASTGKDWEGVGIPPATAVEEGKALQVAQAQAL
                     RRLAAAAPAPEKARLEARATLLAAQVEPVATALPLAAYAGTYGERKVWVEEGRLAFQR
                     EGGPKFRLVAIGPNQFAFEEDPATRLEYRTAGGAATGFDLIRGDGSRVAADRTS"
     gene            7358..7675
                     /locus_tag="VF547_02070"
     CDS             7358..7675
                     /locus_tag="VF547_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX8261639.1"
                     /translation="MSCPHCAVPLVMSERQGVEIDYCPQCRGIWLDRGELDKILERAA
                     ADFGPAAPQAPRAPQAPVAPQAPPYPQGGHYDPRYDQGRHHDQGYGRHHKRRKSWLEE
                     IFD"
     gene            complement(7680..8210)
                     /locus_tag="VF547_02075"
     CDS             complement(7680..8210)
                     /locus_tag="VF547_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8261640.1"
                     /translation="MVGGGEAATEGLMRAVRAGLAVSALAAALAGCVRNPPPEAAAAP
                     TASASPRQMVAARQAAFHLSGAAMGNMKAAIDRGDDPKGQAYAARGVARWARALPTLF
                     PDSTRGIGPSRAKPEIWAAKPDFEAKAAAYAEAAEALAAAAAGGDRAAFAAAHAAAGA
                     TCKACHDLYQAPAAPR"
     gene            complement(8549..8932)
                     /locus_tag="VF547_02080"
     CDS             complement(8549..8932)
                     /locus_tag="VF547_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261641.1"
                     /translation="MEAAIERLAALAFLVTGLSHLLAPRAWVRFFERVEAQGEAAGVW
                     NGLLHLPLGLAVAALHPVWEGPALAVTLIGWVLLAKATAQLCSPALARRSLAMATGER
                     GVARCRIAGAAALAIGAGIGWVAWR"
     gene            complement(8965..9891)
                     /locus_tag="VF547_02085"
     CDS             complement(8965..9891)
                     /locus_tag="VF547_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261642.1"
                     /translation="MPQHNPPEEEGLRPYGPAILLTLVVGFAFLAGVKLFNLPSGAAP
                     AGPPPLPAPGFATMGGFLAYRGFAFLSLVLSAGLAVAAIGAIRLFVAGRRTRDLLLAV
                     LAAEAGVALVLTFAGGSAMRDIADGILCSGGRAFQAWEFCALGPGIGGVQAGLDRWVE
                     AACIVAAGAIVFAIFTVAAARRPRTLPKWIASQERCVTLLLAAGSALLTVRMLLHDSY
                     LRWAFAEFLAVEKPPPPLAAYLGGTATFNGAVETAILGLTWLVALLLMERRPPMAAAV
                     RGDPGGAKFSAYNLPAILTPFLTAVGTGLLRG"
     gene            complement(10001..10387)
                     /locus_tag="VF547_02090"
     CDS             complement(10001..10387)
                     /locus_tag="VF547_02090"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX8261643.1"
                     /translation="MSESKENTNPSSVDPDFDPDEAPEWPDEVWDRAEFAIGDRVIRP
                     ATGTFTRDFTPEEIRARRGRPPLGTEPKVQQSLRLSKAVLDHFRATGPGWQARIDEVL
                     RRHVAEAEAKAGQRRVAEEREPYRGE"
     gene            complement(10347..10619)
                     /locus_tag="VF547_02095"
     CDS             complement(10347..10619)
                     /locus_tag="VF547_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX8261644.1"
                     /translation="MEIGFDPAKREWTLEHRGLDFADAEKVFAGPKFTVEDNRRDYGE
                     NRYLTYGRLDDRLIAIVWTPRGDGRHVISMRKANEREQRKYQSQLG"
     gene            complement(10884..11345)
                     /locus_tag="VF547_02100"
     CDS             complement(10884..11345)
                     /locus_tag="VF547_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261645.1"
                     /translation="MGRKLIAPWALPLALAACTEPAQDVAANRSPPSDAPAPAGGGEG
                     GVRLALLPPADEGPLSALAGRLELDGRCLYLRLPDGSRILPAFQIAGLAWNPSAGAME
                     WRGRAFRPGDPVQLGGAFERGSSTALPWRQAPAPECDASRIFAAWSITPAG"
     gene            complement(11335..12543)
                     /locus_tag="VF547_02105"
     CDS             complement(11335..12543)
                     /locus_tag="VF547_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261646.1"
                     /translation="MSFGGAGHAQETTQPDNTPVQTVEESIAIDAEWYARSQAVTREE
                     AIRRLRIQREMGDVIDRMRRQYQGRLAGIVVDHKPDYRLRVRLTGSEAVAAQSVSLAG
                     STLPIAFETGAPATVDALVDSIQSNLPALKTLFPTLDGIGTDESTSQIVLTVYAPDAV
                     TAAAAKAKESEVAALLKQPFRIETTSLMEKLMDVRGGAKFTSSGGALCTTGFVVKNTS
                     GTTGVATAAHCEGMNTYYNPNGTTIATTIVASSETLDADQDVEVHTSGYVERPEFYAD
                     TTASARVLTGRRYKSSTGYDDNVCHRGEVTKYSCGYVKQTNYTPVDSAGNPLRCGAVA
                     CGPYYVRVEGPELACYHGDSGGPVFASTVAFGLLKTGAASGTAKGQCARMSYMSTDSL
                     PTGWSLLYGP"
     gene            complement(12682..12990)
                     /locus_tag="VF547_02110"
     CDS             complement(12682..12990)
                     /locus_tag="VF547_02110"
                     /inference="COORDINATES: protein motif:HMM:NF022044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX8261647.1"
                     /translation="MRIEVVFRSGDAGRGNAKLNKCPGCAEQVQAEARKCRFCGYDSL
                     GIPSLLVKPDQAGMGCAILAVIGLLIFASGGCRGIGAWVLSLCGRRKSVQNGYFPESI
                     "
     gene            complement(13123..13437)
                     /locus_tag="VF547_02115"
     CDS             complement(13123..13437)
                     /locus_tag="VF547_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX8261648.1"
                     /translation="MILLEKRRRRRRAAAGLPIFGPAVRAEGMEGTGPVRQPLPLSRE
                     VVEYFRAMGPGWQARIDEVLKRHVREEEERARRRAAEAAGAKARVAEEREAFRGEGEE
                     GG"
     gene            complement(13509..13721)
                     /locus_tag="VF547_02120"
     CDS             complement(13509..13721)
                     /locus_tag="VF547_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261649.1"
                     /translation="MPDILISNERWKATSNLSLAFCGGLLAASAARVWEIGDLDGSAS
                     VWLVVGLAFGFFAWLTLGFLVPEGAQ"
     gene            14030..15994
                     /gene="ftsH"
                     /locus_tag="VF547_02125"
     CDS             14030..15994
                     /gene="ftsH"
                     /locus_tag="VF547_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8261650.1"
                     /translation="MSDQNPRKPDKDPSSPWMKSLFIWAGILLALILFVQLVDGGGSR
                     GAAAGTMAYSEFLNRVDEGSVKEVSIGKETITGKLASGETFRTHFIPDPQLTGRLREK
                     GVAFQGEPEPQTSVWLILLYQSLPFLLILGIAFFIMRQMQKNAGSGAMGFGRSKAKLL
                     TEKSGRVTFDDVAGIDESREELQEIVDFLKDPTKFARLGGKIPKGALLVGAPGTGKTL
                     LARAVAGEANVPFFTLSGSDFVEMFVGVGASRVRDMFEQAKKNAPCIVFIDEIDAVGR
                     HRGAGLGNGNDEREQTLNQLLVEMDGFEANEGIIIIAATNRPDVLDPALLRPGRFDRQ
                     VVVPRPDIEGREKILAVHMKKVPLAPDVDPRTIARGTPGFSGADLANLVNEAALLAAR
                     KGKRLVAMSEFELAKDKVMMGAERKSMVMTEDEKKATAYHEAGHALVSLHVPGCDPLH
                     KVTIIPRGRALGVTWNLPERDRYSMSMKQMKARLALCFGGRIAEQLIYGVEELNTGAS
                     NDIQQATDMARAMVMEYGMSPKLGWLRYRDNQEEVFLGHSVARSQSVSEETARLIDSE
                     VRRLIEEAEATARNVLTENLDELHRLAGALLEYETLTGEEAKKAIKGEDIGREDPDSR
                     RPAMPVAGTSIPSTRRPRGGGIGGPAPQGA"
     gene            16124..16489
                     /locus_tag="VF547_02130"
     CDS             16124..16489
                     /locus_tag="VF547_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261651.1"
                     /translation="MKLLAALSLAVVPLGAAQAMDVASFLAKADALQKKGMMALMSSD
                     YKLLKNEIVTHSQTLRNERLAAQKAGRRPAYCPPAKSGLKSDEILAAFRSIPAAQRPR
                     TDVKDALRALLARKFPCRS"
     gene            complement(16493..17692)
                     /locus_tag="VF547_02135"
     CDS             complement(16493..17692)
                     /locus_tag="VF547_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX8261652.1"
                     /translation="MSGGLESVGEAVTGGLAARAVEPGAGEGRGEGGGACLNCGTALL
                     GPHCHQCGQAGHVHRTLTAFWHDLAHSVLHFDGKLWRTLPMLALRPGELTRRYVAGER
                     AGFVSPMALFLFSVFLMFAVFSSVGGPFGGGSTPEPDRSPAQERAEARRDFDRERAEA
                     LAELRELRAERARRAAARQPTASVDGRIQRLQARLALEEKLFNQALDLIAAEEKSEAD
                     RAKAEAENTVAGPARKAVEAADSNEMVLVSGADSLNAWFRNAYRKAKENPSLLAYKLQ
                     TNAYKFSWALIPISVPFLWLLFLHRARYRRFKAYDHTVFVTYSIAFMSLGLIALSLLR
                     LVGMPAVFAVLAITFVPPVHIYRQLRGAYALSRWSAVWRTFVLINFAFIAASLFFTLL
                     LALGLLG"
     gene            17869..18015
                     /locus_tag="VF547_02140"
     CDS             17869..18015
                     /locus_tag="VF547_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261653.1"
                     /translation="MTKPLKELVASTGDQGLKVFVRTASRELTLDDLEVIAGGRGARW
                     DPYG"
BASE COUNT         2917 a         6312 c         6395 g         2592 t
ORIGIN      
        1 ggtgcaggtg agcccggaaa gataccagac gactggacag gcggcgccct cgtcggcctg
       61 cggcggcacg aagacggaga aggtcatgtc ggtgccggtc gcacggctgg catgcttcac
      121 gacatgctgc gtgccgccgt gcgagcgggc gacggagatt gtgtcgaggg tcatcatcca
      181 ctccgttcgc cctgagcctg tcgaagggcc tgctttcctg gaaagaaaat aaggggcttg
      241 gacaggctcg gcccgaacgg gttcaattgc ctgtcagtag atcaccacgc tcctgatgct
      301 ctcccccgca tgcatcaggt cgaaagcctt gttgatgtcg tcgagcggca tcgtgtgggt
      361 gatgagcggg tcgatggcga tccggccgtc catgtaccag tcgacgatgc gggggacgtc
      421 ggtgcggccg cgggcgccgc cgaaggcgct gcccttccag acgcgcccgg tgaccagctg
      481 gaagggccgg gtggcaatct cgcggccggc ctcggcgacc ccgatgatgg tcgagacccc
      541 ccagccccgg tggcagcatt cgagggcggc gcgcatcacg tcggtgttgc cggtgcagtc
      601 gaagctgtag tcggcgccgc cgccggtgag gtcgaccagg gcctggacga gcctgtcggc
      661 cccgacctcg ttcgggttga cgaaatcggt catcccgaac gcctcggcca tggcgcgctt
      721 ggctgggttg atgtcgaccc cgacgatcct ggcggcgccg accagccggg cgccctggat
      781 gacgttgagg ccgattccgc cgaggccgaa gacgacgacg gtcgaccccg gctcgacgcc
      841 ggcggtccag atcaccgcgc cgacgccggt ggtgactccg cagccgacgt agcagatcct
      901 gtcgaagggc gcgtcctcgc ggaccttcgc gaccgcgatc tcgggcatca cggtgaagtt
      961 cgagaaggtc gagcagccca tgtaatggtg gatcgtctcg ccgccgaggc ggaagcggct
     1021 ggtgccgtcg ggcatcacgc ccttgccctg ggtggcgcgg atcgccgtgc acaggttggt
     1081 cttgcggctg aggcaggatc tgcactgccg gcattcgggc gtgtagagcg ggatgacatg
     1141 gtcgcccggc cggaccgccg tgacgccttc gcccacctcg cggacgacgc cggccccctc
     1201 gtggccgagg atggagggga aggtgccctc gctgtccttg ccctccagag tgtaggcgtc
     1261 ggtatggcag atgccggtcg ccatgatctc gaccagcacc tcgcccgcct tggggccgtc
     1321 gagatcgacc tcgtggatct cgagcggacg gttgggggcg acggcgacgg cggcgcgggt
     1381 cttcattctt cctcctttcc tccccggaaa gggggagggg gaccagccga aggctggtgg
     1441 agggccggcc cctgtgcaac ctcctgaggc cgggccccca accaccgcac ttcgtgcggt
     1501 ccccctcccc ggtccgggga ggagatgtca agccgacggc attcgactac aaggcgtgac
     1561 gggaggattt cgccatgttg atcgcgctcg ccgccgccgc cgccttccag tcgtccgccc
     1621 cgccgccgcc gaccccgccc tgctcgaccc ccgaataccg ccagctcgac ttctgggtcg
     1681 gcgagtggga cgcggagttc gccctgccgg gcggcaagac cgggcgggcg acgaaccgaa
     1741 tcacccgcga cgagttcggc gactgcgtga tcgccgagca tttcagccag cccgacaccg
     1801 gttatctcgg cgcgtcgttc agcacctggg atcggctcaa gaagaaatgg gtccagacct
     1861 gggtcgacaa tggcggcgcc tacatcacgc tcgccggagg accggtcgaa gggcagccct
     1921 tcagcttcga gctggtcacg ctcgacccgc gcggtccgca gcaggtccat agccggatga
     1981 tctggcagga tgtgaagccg aacagcttca cctggcgctg gcaggcgcgc cagcccgacg
     2041 gaagctgggc cgacagctgg gtgatcaaat atacgaggcg aaagtgaaaa aaggggacac
     2101 gtactttttc acgaaaaaag tacgtgtccc cattcctcgg tgcgagctgc ccctccacca
     2161 ggctccgaat ggtccccctt cccatgcttc gcatggggag gatgatcagc gcttccactt
     2221 cctgcggtcg gcccagattg ccgccgccgc gttgtgcccc ggcgccccgg tcacgccccc
     2281 gcccggatgc gcgcccgatc cgcacagata gagccccgga agcggcatcc ggtagtcggc
     2341 atggccgagc atcgggcgcg ccgagaagag ctggtcgagg cccatcttgc cgtggaagat
     2401 gtcgcctctc ggcactccga aacgctgctc gaggtgccag ggggagaggg ccaggcggcc
     2461 gaccacggag gccttgaagc cgggcgcgta gcggtcgaca tggtcgatga catggtcggc
     2521 ggcctcctcg acgcgggcct cccagccccc ttccacctcg taggggaaat gctggcagaa
     2581 gaggctggcg acatggcctc ccggcggcgc caggctgtcg tcgagggtcg aggggatcag
     2641 catctcgacg atcggctcgc gcgcccagcc ctcggtccgg gcgctggtgt aggcgcggtc
     2701 catgtagcgc agcgaggggg cgatgatgat cccggcggtc agatggtcgc ccggctcggt
     2761 caggctggcg aagcggggga gctcggacaa ggcgacattc atccggaagg tggcggattc
     2821 ggcggcccag ccggccatcc ggcgcgagat ctcggcggcg acggcgccgt cggggagaag
     2881 gctgccgaac aggaagcgcg gattgacgtt ggcggccacc gccttcgccc gccaggccct
     2941 gccgcccgcc accgcgcccg ccgcccggcc gcgctccacg atgatctcct cgaccggcga
     3001 atcgaggacg atctcgacgc cgcgctcgcg cgccgccttg gccatggcct gggtgatcgc
     3061 gcccatgccg ccgatcgcat ggccccacgc gccggccacc ccggccgcct ccccgaacag
     3121 atggtggagg aggacgtagg cggagccggg gtcgtagggg ctggcataat tgccgaccac
     3181 ggcgtcgaag ccgaacagcg cctggacgat gtcggtctcg aaatagcggc cgaggatgtc
     3241 ggcggcggac ttggtgaaga agtcgagcaa gttgcgctgc tcctccgcgc tcatgccgcg
     3301 aacggcgttg ccgagcttca gggccgcaag cacgtccgcc agcccgccgc cggcattggg
     3361 cggagcgcgc aatatccagt cgcgcaggat ggcgacgacc gagcgcagcg cggccgaata
     3421 agcgtcgtag ccgtcagcat cggcgggcga atgccgggcg atctcgcgcc gggtgaggcc
     3481 gcctgcgccg gagaggagat ggtccggtcc gagcgtcggc aggaaattgt cgatcctccg
     3541 gagcacgacc ttcaggccgt gctcgtagag ccgcatgtcc tcgatcacct tcgggttgag
     3601 gaggctgacg gtgtagctgg cggtcgagtt gcggaatccg gggtggaact cctcggtcac
     3661 cgccgccccg ccgacgacgc cgcggcgctc gaggatcgtc accttcaggc ccttggcggc
     3721 cagataatag gcgcagacga ggccgttatg gccggcgccg atgacgagta cgtcggtgtc
     3781 gggggtcatg gccgcggcta tacccagcgg ccccgcgccc gtccatttac ccggtcttca
     3841 tccgctccgc ttaggccggg gccgggggag agctttctat ggtgatgaag atactggtct
     3901 atctggcgtc gccgatcacg atctcgctga tcctgatcgg cgcgatgcgg atcgacggcg
     3961 aattctcgcg cgacatcccc cagccgccgg ccgtcgtggc ggcggcgctc gagaccgtcg
     4021 acgtcgtcga ccagcctcat gcgcagaagg cgctccgcga ggccggcgag aaaagggttc
     4081 cgcagatcct cgcgcagcgc gacccggacg gcttcagctg gttcgtgatg agccgcggca
     4141 agtccgtgct gaggatgaag gcgcagctcg tgccctccgc cgacggcgcc tcgacccatg
     4201 tcagcaccga ggtggagccg ggcgaggtcg aggccgggcc cgacgtgccg ccgctgttcg
     4261 cctcccgctc cgaggtcgag ccgctgttcg cgatcgcggt ggagcgggcg ctgggcgact
     4321 atatcccccg atcggaacga agcctcgcca gcctgcagga gctgcccgcc cgctacaaga
     4381 cgcaggaaga gatgaacgcc gaatatgagg ccaggcatcc ggattcgagc ttctagccgc
     4441 cgccgcgcag ggccgcggtc cgtcgaaggg aaagcaaagt gttgaagatg ctcctgaaga
     4501 atttcggctt cttcatcctg ttcgtgacgg gcacggccta cgtctcgggc ggcctcgcag
     4561 gcggcgccta ttcgcgcgag gtggagcgcc cgccggccga ggtgatggcg gcgctggccg
     4621 atctcgacat caccgcccag ccgggctctc cgggcaccga tccgtcgcga tccggcggag
     4681 tccagcccgt cttccggctc gagcggtccg gggaccggat gacctggacc gtgatgagcg
     4741 gcgagcaggt ggccgtccgg atgatcgcgc tcgtcgagcc cgtccgggac ggcaagcaca
     4801 gccgggtcac cgcctcggtg gagcgcggcg acgctcccga cgacgtcgtc tcccccgcct
     4861 tccgctcgga ggggatcacg atgggcctgt tcgggatggc gctggaaggg gagctcaacc
     4921 ggctcaccgc gccgccgccg gccgaccagg ccagatgcga tgcgctgatg gcggagttcg
     4981 aggcgggcaa catggcggcg ggcttcgccg aaaggcccga caacctgacc gaggccgtcg
     5041 gcaacaccgc gaagatcgcc gtccggctgc acgccatgga ggcggagctc cgccgcaacg
     5101 gctgcccgac ggagccgagc cgcgatttcg tcgaggtgtc cgacgagatg agcaatggcg
     5161 gccagggggg ccaggctccc atcgccgaca ttcctgtcga aggaatcagc ttcgagcccg
     5221 gcaagccgat ggtcgacctt tcgcagggca agcgccggaa ctaggcggtc gccgttggga
     5281 tcaccgccct cgcgcgggcc tggctagtcg agcggcaagg cgaggcttgc cttcaggcgc
     5341 ctcttgacga agctggtctc gtagcggcgg acagcccgct ggccggcgag aaggtcgtcg
     5401 gcgaacaggt tgaacgcctc catgtcggcg accacgacga gcaggaggat gtcgaacgcc
     5461 cctgaaatct cgaggcagaa ctggacgttc tgcgaagccg cgagctggcg cttgaagctg
     5521 gcgacctcgg tgagggcgtg ccgttcgagc tggacgtgga tgacggcgga cagcggatgg
     5581 cccgccgcct gctcggagac caatgcgaca tcggcgacga cggcgccgct gtcgcgcagc
     5641 cggcgcagcc ggcgggccac ggccgagggc gagcggccga cccggtcggc gagcgcctcc
     5701 gcggtcatca gattgtcctg ctggaggcag gcgagcaatt tgcggtcgag cccgtcgagt
     5761 cccgacatcg ggcgattcgt cccttcattt tgcaagaaac cggcagcggc gctgcgatct
     5821 ttgccggaat ctgccgtccg agccaagcga tagtccgact gacggctatg acggagaggc
     5881 agatgaaccg gcacggaatc gcactgggta tcgcggccgc ggccctggcc ggggcggcgg
     5941 gcgcgccggc ccagccggcc cggacgggcc cggcgccggc ggcttcgccc gcgcccgccg
     6001 ccggcgaagc cgtggtcgcg gagcttcgtc gggtgctggc cgccaattac gtgcttcccg
     6061 aggtccggcc gaagctcgac tccgcgctcg ggcgcggact ggccgccgga cgctacgcca
     6121 cgaccgatcc ggccgtcctg gtcgagcggg tcaatgccga tctcgccgcg gtcaccaagg
     6181 acaagcatct gggcctgcac tatgatccgc gccaggccgc cgagatcgcg gcggcgccgc
     6241 agggccaggc cgaggacggg ccgcccacgc ccggccagat ccgccaggct cggctgcgca
     6301 accacggctt cgtcgagatg aaggtgctgc caggcaatgt gcgctacgtc gacctccagg
     6361 gcttcgtctg ggtcggcccc gaaagcgccg cggcctacga caatgcgatg cgcttcctcg
     6421 ccggcggcga cgcggcgatc gtcgacctca ggaagaacgg cggcggcagc ccggaggcgg
     6481 tccagtatct ggtcagccat ttcatggagc cgaaccggcc gctggtgacc ttccacatgg
     6541 gcgcgaccca ggtcgaccgg ctggcgaccc tggccgagct tcccgccgga cggatggtcg
     6601 gcaagccgct ctacgttctg accagcggcc acaccgccag cgccgccgag gagttcgccg
     6661 gccacgtcgc cggctaccgg ctgggcgagc tgatcggcga gacgaccgcg ggcgccggct
     6721 tccgcaacag cttcttcgcc cttcccggcg gctatgtggt cagcgtctcg gtcgggcgcg
     6781 cggtgctcgc ctcgaccggc aaggattggg agggcgtcgg aattccgccg gcgacggcgg
     6841 tcgaggaggg caaggcgctc caggtcgccc aggcccaggc cttgcgccgg ctggccgccg
     6901 ccgcgccggc gccggagaag gcccgcctgg aggcccgggc gacgctgctc gcggcccagg
     6961 tcgagccggt cgcgaccgcg ctgccgctgg ccgcctatgc cggcacctac ggcgagcgca
     7021 aggtctgggt ggaggagggg cggctcgcct tccagcgcga gggcgggccg aaattccgac
     7081 tggtcgcgat cggccccaac cagttcgcgt tcgaggagga cccggcgacc cggctcgaat
     7141 accggaccgc cggcggcgcc gcgaccggct tcgacctgat ccgcggcgac ggcagccggg
     7201 tcgcggccga ccgcacgtcc tgaccgccgc tccagccgcg gcgcgatccg ggcccgggcc
     7261 cgatccgagg cggcgggagg ccgcgcattg ccaatcgccc gcgaaggcct atctgcccgg
     7321 cagccgaccc gacgggagac gaatgatgcc tggccccatg tcctgccccc attgcgccgt
     7381 tccgctggtg atgagcgagc ggcagggagt ggagatcgac tattgcccgc aatgccgcgg
     7441 catctggctc gaccgaggcg agctggacaa gatcctcgag cgcgccgccg ccgacttcgg
     7501 gcccgccgcg ccccaggcgc cgcgcgcgcc ccaggcgccc gtcgcgccgc aggccccgcc
     7561 ctacccgcag ggcggccatt acgacccgcg ctacgaccag ggccgccacc acgaccaggg
     7621 ctacggccgc caccacaagc gccgcaaatc ctggctcgag gagattttcg actgatccgc
     7681 tagcgcggcg ccgccggcgc ctggtagagg tcgtggcagg ccttgcaggt cgccccggcc
     7741 gcggcgtggg cggcggcgaa ggcggccctg tcgccgcccg cggcggcggc ggcgagcgcc
     7801 tcggccgcct cggcataggc cgccgccttc gcctcgaaat cgggcttggc ggcccagatc
     7861 tccggcttcg cccggctcgg gccgatcccc cgggtcgagt ccgggaacag ggtcggcaga
     7921 gcccgcgccc agcgcgccac gccgcgggcg gcataggcct ggcccttggg gtcgtcgccg
     7981 cggtcgatcg ccgccttcat gttgcccatc gccgcgccgg agaggtggaa ggcggcctgc
     8041 cgggcggcga ccatctgccg gggcgaggcc gaagccgtcg gcgccgcggc ggcctcgggc
     8101 gggggattgc ggacgcatcc cgccagcgcc gccgccaggg ccgaaaccgc cagacccgcc
     8161 cgaaccgccc gcatcagacc ctccgtcgcc gcctccccgc cgcctaccat gccgccccgt
     8221 ccgccgccat cccgccgcga ggaggatgtt ggcaaaggtg ccgcggagcg tgagtccggc
     8281 ggctgcggcg ggctaaggtc ttgagcggga agcggtggac gggtccctcc tttcatcgga
     8341 ttccgccaac aagcccagag ggcgggacgg atcgcccggg tccggcgcga atcgccggac
     8401 ttcgccagaa cgcgcagggg cggttgcggg cgccgccggc cgccgtcctc gagccgcgac
     8461 ggcgcgtggg cggccgaccg gcgcatccgg gcgcctccgg ccaggcgcga gccgatcggt
     8521 gccggcgcgc tgcaagaatt tgcaaaggtc acctccaggc cacccagccg atccccgccc
     8581 cgatcgccag cgccgccgcc ccggcgatcc ggcaccgagc caccccccgc tcccccgtcg
     8641 ccatggccag gctccgccgc gccagcgccg gcgagcagag ctgcgcggtg gccttcgcca
     8701 gcagcaccca gccgatcagg gtcacggcaa gcgccggccc ctcccagacc ggatggagag
     8761 cggcgacggc gagcccgagc ggcagatgca gaagcccgtt ccagaccccc gccgcctccc
     8821 cctgagcctc gacccgctcg aagaagcgca cccaggcccg cggcgcgagc aggtgggaca
     8881 ggccggtgac gaggaacgcc agcgccgcaa gcctttcgat cgccgcttcc atcgcgccct
     8941 cccttccggc acccgcggcg cccttcagcc cctcagcagg ccggtgccca cggcggtcag
     9001 gaacggagtc aggatcgccg gcaggttgta ggccgagaat ttcgccccgc ccggatcgcc
     9061 ccggacggcg gcggccatgg gcgggcggcg ctccatcagc agcagagcga ccagccaggt
     9121 gaggccgagt atggcggtct cgacggcgcc gttgaaggtc gccgtcccgc ccagataggc
     9181 ggcgagcggc ggcggcggct tctcgacggc gaggaactcg gcgaaagccc agcgcagata
     9241 ggaatcgtgg aggagcattc ggacggtgag gagcgccgag ccggcggcga ggagcagggt
     9301 cacgcaccgt tcttggctgg cgatccattt gggcagggtg cgcgggcggc gcgcggcggc
     9361 gacggtgaag atggcgaaga cgatcgctcc ggcggcgacg atgcaggccg cctcgaccca
     9421 gcggtcgagc ccggcctgaa ccccgccgat gcccgggccg agggcgcaga attcccaggc
     9481 ctggaaggcg cgcccgcccg agcacaatat cccgtccgcg atgtcgcgca tcgccgagcc
     9541 gcccgcgaag gtgagcacga gcgcgacccc cgcctccgcg gcgagcacgg cgaggaggag
     9601 gtcgcgggtc ctccggccgg cgacgaacag ccggatcgcc ccgatcgcgg cgacggcgag
     9661 gccggccgac aggacgagcg agaggaaggc gaagccgcga taggcgagga agccgcccat
     9721 cgtcgcgaag ccgggggcgg ggaggggagg cgggccggcg ggcgcggcgc cggacgggag
     9781 gttgaacagc ttcacccccg cgaggaaggc gaagccgacg acgagggtga ggaggatggc
     9841 cggcccatag gggcgcaagc cttcttcttc cggcgggttg tgctgcggca tggtcgggcc
     9901 ccctcgcggg gggtgtttcg cacagccgcg gcgggctgcc aacgggggtg cggggggcgg
     9961 cgggggcggg gcggggccga tcagggccgg ggtgccggcg tcattccccc cgatagggct
    10021 cccgctcctc cgccacccgc cgctggcccg ccttcgcctc cgcttcggcg acgtgccgcc
    10081 tcagcacctc gtcgatccgg gcctgccagc ccggccccgt cgcccggaaa tggtcgagca
    10141 ccgccttcga caaccgaagc gactgctgca ccttcggttc ggtgcccaac ggcggccggc
    10201 cgcgccgggc gcggatttcc tcgggggtga agtcgcgggt gaaggtccca gtcgccggcc
    10261 ggatcaccct gtcgccgatg gcgaattcgg cccggtccca gacctcatcc ggccattccg
    10321 gcgcctcgtc gggatcgaaa tcgggatcaa ccgagctggg attggtattt tctttgctct
    10381 cgctcattgg cttttctcat tgaaattacg tggcggccat cgccgcgggg cgtccagacg
    10441 atcgcgatca gccgatcatc gagccgtcca taggtcaggt agcggttctc gccataatcc
    10501 cgtcgattgt cctccaccgt gaacttcggt cccgcgaaca ccttctcagc gtcggcgaaa
    10561 tcgagcccgc gatgctcgag cgtccactcg cgcttggcgg ggtcgaagcc gatctccacg
    10621 gcctcgatct acgcccatag cacggccacg gcaatatgta gcagcgaaaa tgcgaagatc
    10681 ggtttttggc gaaacggaaa tggcgacctt gcctggcatt gcgcctgccc ctgaacctcg
    10741 accggctcga agaagagcac ccaggcgcgg ggcgcggcga ggtgcgggag gccagtgacg
    10801 acgaacgcca gagccgcccc gggtaaatcc tattttcgat gcgcgtagga aattcgaggg
    10861 gactcggaca ttggcgggca tgctcagccg gcgggcgtga tgctccatgc cgcgaagatc
    10921 cgcgaggcgt cgcactccgg ggcgggggcc tggcgccagg gcagcgccgt ggacgagccg
    10981 cgctcgaacg ctccgccgag ctgcaccggg tcgccaggcc ggaaagcgcg gccgcgccac
    11041 tccatggcgc cggccgacgg gttccaggcg aggcctgcga tctggaaggc gggaaggatc
    11101 cggctgccgt cggggaggcg cagatacagg cagcgcccgt cgagctcgag cctgcccgcg
    11161 agcgcggaga gcggcccttc gtcggccggc gggagaagag ccaggcggac cccgccctcc
    11221 ccgccgcccg cgggagcggg ggcgtcggag ggcggggacc ggttggcggc gacatcctgg
    11281 gccggctccg tgcaggctgc cagcgccagc ggcagagccc agggcgcgat cagcttacgg
    11341 cccataaagg agggaccatc cggtcggcag gctgtcggtg gacatgtaag acatgcgcgc
    11401 gcactggcct ttcgcggttc ccgaagctgc ccccgtcttg agcagcccga aggcgacggt
    11461 cgaggcgaag acggggccgc cgctgtcgcc atgatagcat gcgagctcgg ggccttcgac
    11521 ccttacgtaa taaggcccgc aggcgaccgc gccgcatctc agcggattcc cggcgctgtc
    11581 gaccggcgtg taattggtct gcttcacata gccgcagcta tatttcgtga cctctccgcg
    11641 gtggcagaca ttgtcgtcat agccggtgct gctcttgtag cggcggccgg tcaggacccg
    11701 cgccgaggcg gtggtgtccg catagaattc cggccgctcg acatagccgc tggtgtgaac
    11761 ctcgacgtcc tggtcggcat cgagcgtctc ggagctggcg acgatcgtcg ttgcgatggt
    11821 cgtcccgttc ggattgtaat aggtattcat gccctcgcaa tgcgccgcgg tggcgacacc
    11881 ggtcgtgccg gacgtgttct tgacgacgaa tccggtggtg caaagggcgc cgcccgagga
    11941 agtgaacttc gcgccgccgc gcacgtccat cagcttttcc atcaggctcg tggtctcgat
    12001 ccggaacggc tgcttgagca gggccgccac ctcggactcc ttcgccttgg ccgcggccgc
    12061 cgtcacggca tcgggggcgt agaccgtcag aacgatctgg ctggtgcttt cgtcggtgcc
    12121 gattccgtcg agcgtcggaa acagggtctt gagggcggga aggttgctct ggatcgagtc
    12181 gaccagagcg tcgaccgtcg ccggagcgcc ggtctcgaac gcgatcggca aggtcgaacc
    12241 cgccagcgag acgctctggg cggcgacggc ctcgcttccg gtcagcctga ccctcagccg
    12301 gtagtcgggc ttgtggtcga cgacgattcc ggcgagcctg ccctgatact ggcgccgcat
    12361 ccggtcgatg acatcgccca tctcgcgctg gatccgcagg cggcgaatcg cctcctccct
    12421 ggtgacggcc tgcgagcgcg cgtaccattc ggcatcgatg gcgatcgact cctcgaccgt
    12481 ctggaccgga gtattgtcag gttgagtggt ttcctgggcg tgacccgctc caccgaacga
    12541 catcagacct atagtcaatg cagcaacaga tgcagccctg gccgcgaaca cgtgaatccg
    12601 tttcatatta gcctctacgc aagaaccgga ggaaaggctt tcctccaact gcaagaagct
    12661 atcagtatcc tatttgttcc tttaaattga ctccggaaaa tatccatttt ggacggattt
    12721 tcgtcgtccg cagagcgaga ggacccaagc gccgatcccc cggcagccgc ccgacgcgaa
    12781 aatgagcaag ccgatcaccg ccaggatggc gcagcccatc ccggcctggt caggctttac
    12841 cagcagcgac gggatgccga gagaatcata gccacaaaac cggcacttgc gcgcctcggc
    12901 ctgcacctgc tcggcacagc cgggacactt gttcagtttc gcattgcccc tgcccgcatc
    12961 cccggaccgg aataccactt cgatccgcaa ccggaatccc ccgttcaccc gcgcccgcta
    13021 caagccgggc gacattgcca aagccccgcc aaaccctgtt ttggaggcga agcggctatg
    13081 agcggccgag agcggggcgt cgagtgggcg ccccaggaga catcagccgc cctcctcacc
    13141 ctccccccga aaagcctccc tctcctccgc cacgcgcgcc ttcgcgcccg ccgcctccgc
    13201 cgcccgccgc cgggcccttt cctcctcctc gcgcacgtgc cgcttcagca cctcgtcgat
    13261 ccgcgcctgc cagcccggcc ccatcgcccg gaaatactcg accacctcgc gcgacagagg
    13321 cagaggctgc cggaccggcc ccgtcccctc catgccctcc gcccgcaccg ccggcccgaa
    13381 gatcggcagc cctgccgccg cccgccgccg gcggcggcgc ttctcgagca aaatcatcgt
    13441 cgcgaatatg gctaggacga gaagcgggac cgcgatgggc gcgaatgggc cgagcgggat
    13501 ataccagttc actgcgcgcc ctccggcacg agaaagccga gcgtcagcca cgcgaagaaa
    13561 ccgaatgcca gaccaacgac aagccagacg gaggcggacc catcgaggtc cccgatctcc
    13621 cacacccgcg ccgcgctggc cgccagcagg ccgccgcaga aggccaggct gaggttcgag
    13681 gtcgccttcc atcgttcgtt gctgatcagg atgtccggca taacatcccc gctcgattac
    13741 tgaaaaacga atagaaccga tttcagttca atgcttttag acgatctccc gatcgtcggc
    13801 aagcggggcg gatgccgcgc ctcggcgccg cgcggtccag gggcccccct gtcccgcctt
    13861 cgcccggcca cggcccgccg cgcggtaaac aggttgttca gccggacgcg ctaagttgcg
    13921 cttcccccgc aaaaccctat tttagcgggt gaacaaggaa ccaacggtac ctcctgggct
    13981 cccctccttt ttcgggaccc catgaaagta ccactttcat gggacgatca tgagcgacca
    14041 gaacccccgc aagcccgaca aggacccctc cagcccgtgg atgaagagcc tcttcatctg
    14101 ggccgggatc ctgctcgcgc tgatcctgtt cgtgcagctc gtcgacgggg gcgggtcgcg
    14161 tggcgccgcg gcggggacga tggcctattc ggagttcctc aaccgggtcg acgaggggtc
    14221 ggtcaaggag gtgtcgatcg ggaaggagac gatcaccggc aagctcgcca gcggggagac
    14281 gttcaggacc cacttcatcc ccgatccgca gctgaccggg cggctccgcg agaagggcgt
    14341 cgccttccag ggcgagccgg agccgcagac ctcggtgtgg ctgatcctgc tctaccagtc
    14401 gctgcccttc ctgctgatcc tcgggatcgc cttcttcatc atgcggcaga tgcagaagaa
    14461 cgcgggctcg ggggcgatgg ggttcgggcg gtcgaaggcg aagctgctga ccgagaaatc
    14521 ggggcgggtg acgttcgacg acgtcgccgg gatcgacgag agccgcgagg agctgcagga
    14581 gatcgtcgac ttcctcaagg acccgaccaa gttcgcccgg ctgggcggca agatcccgaa
    14641 gggggcgctg ctcgtcggcg cgcccggcac cggcaagacg ctgctggcgc gggccgtcgc
    14701 cggcgaggcc aacgtgccct tcttcaccct ctcgggctcc gacttcgtcg agatgttcgt
    14761 gggcgtgggc gcgagccggg tgcgcgacat gttcgagcag gccaagaaga acgcgccctg
    14821 catcgtcttc atcgacgaga tcgacgccgt cggccggcac cgcggcgcgg ggctgggcaa
    14881 cggcaatgac gagcgcgagc agactctgaa ccagctgctc gtcgagatgg acggcttcga
    14941 ggcgaacgag ggcatcatca tcatcgccgc gaccaaccgg cccgacgtgc tcgatcccgc
    15001 gctgctgagg ccggggcggt tcgaccggca ggtcgtcgtg ccgcggcccg acatcgaggg
    15061 gcgcgagaag atcctcgccg tccacatgaa gaaggtgccg ctggcgcccg acgtcgatcc
    15121 gcgcaccatc gctcggggga cgcccggctt ctccggggcg gacctcgcca acctcgtcaa
    15181 cgaggcggcg cttctcgccg cgcgcaaggg caagcgcctg gtcgcgatgt cggagttcga
    15241 gctcgccaag gacaaggtga tgatgggcgc cgagaggaag tccatggtga tgaccgagga
    15301 cgagaagaag gcgaccgcct atcacgaggc cggccatgcc ctcgtctcgc tgcacgtgcc
    15361 gggctgcgac cccttgcaca aggtgacgat catcccgcgc gggcgggcgc tgggcgtgac
    15421 ctggaacctg cccgagcggg accgttactc gatgtcgatg aagcagatga aggcgcggct
    15481 cgcgctctgc ttcggcgggc ggatcgccga gcagctgatc tacggagtcg aggagctgaa
    15541 caccggcgcc tcgaacgaca tccagcaggc gaccgacatg gcccgggcga tggtgatgga
    15601 atatggcatg tcgcccaagc tcggctggct tcgctaccgc gacaaccagg aggaggtgtt
    15661 cctcggccac agcgtggcga ggagccagag cgtctccgag gagacggcgc ggctgatcga
    15721 ctcggaggtg cgccggctga tcgaggaggc cgaggcgacg gcgcgcaacg tgctcaccga
    15781 gaatctcgac gagctccacc ggctcgccgg ggccctgctc gaatatgaga ccctgaccgg
    15841 cgaggaggcc aagaaggcga tcaagggcga ggacatcggc cgcgaggatc ccgattcgcg
    15901 ccggcccgcc atgcccgtcg cgggcacctc gatcccctcg acccggcggc ctcgcggcgg
    15961 cggcatcggc ggcccggcgc cccagggggc gtagcgcgct caggtcctcc ccgcgatttc
    16021 cctccccggc gagggccggg gaggattgaa gggtccgttc aaccggcgtt caggcgccgg
    16081 gccgtccctt cccggcgcca cgtgggaggg aaatatcgtg agaatgaagc tgcttgccgc
    16141 cttgtcgctg gccgtcgtcc cgctcggcgc cgcccaggcg atggacgtcg cctcgttcct
    16201 ggccaaggcc gacgcgctcc agaagaaggg catgatggcg ctgatgtcga gcgactataa
    16261 attgctcaag aacgagatcg tcacccactc ccagaccctg cgcaacgagc ggctcgcggc
    16321 ccagaaggcc ggccgccggc ccgcctattg cccgccggcc aagagcgggc tcaagagcga
    16381 cgagatcctc gccgccttcc gctcgatccc ggcggcgcag cgtccgcgca ccgacgtcaa
    16441 ggacgcgctc cgggcgctgc tggcgcgcaa attcccctgc cgaagctgag gctcagccga
    16501 ggaggccgag cgcgagcaga agggtgaaga acaggctcgc ggcgatgaag gcgaagttga
    16561 tcagaacgaa ggtgcgccag accgcgctcc accgcgacag cgcataggcg ccgcgcagct
    16621 gccggtagat gtggacgggc ggaacgaagg tgatcgcaag cacggcgaac acggccggca
    16681 tcccgaccag ccgaagcagg ctgagcgcga tcaggcccag gctcatgaag gcgatcgaat
    16741 aggtgacgaa gacggtgtgg tcataggcct tgaagcgtcg gtagcgcgcc cggtggagga
    16801 acagcagcca caggaaaggc accgagatcg ggatcagcgc ccaggagaat ttgtaggcgt
    16861 tggtctgcag cttgtaggcg agcagcgacg gattctcctt ggccttcctg taggcgttgc
    16921 ggaaccaggc gttgagcgaa tccgcgcccg agacgaggac catctcgttc gagtccgccg
    16981 cctcgaccgc ctttctcgcc ggcccggcga cggtgttctc cgcctcggcc ttcgcccggt
    17041 ccgcctcgct cttctcctcg gcggcgatca ggtcgagcgc ctggttgaac agcttctcct
    17101 cgagcgcgag cctggcctgg aggcgctgga tccggccgtc gacggaagcg gtcggctggc
    17161 gcgccgccgc ccgccgggcg cgctcggccc gaagctccct gagctcggcc agggcctcgg
    17221 cgcgctcgcg gtcgaagtcg cgccgggcct cggcccgctc ctgggccggg ctgcggtccg
    17281 gctcgggcgt cgacccgccc ccgaacggcc cgccgaccga cgagaagacc gcgaacatca
    17341 ggaagaccga gaagaggaac agggccatcg gcgacacgaa gccggcccgc tcgccggcga
    17401 cgtagcgccg ggtcagctcg cccggccgaa gcgccagcat cggcagcgtc cgccacagct
    17461 tgccgtcgaa atggaggacg ctgtgggcga ggtcgtgcca gaaggcggtg agcgttcggt
    17521 ggacgtgccc cgcctggccg cactggtggc aatgggggcc gagcagagcg gtcccgcaat
    17581 tgaggcaggc gccgccgcct tccccccggc cttcgccggc gcccggctcg accgcgcgcg
    17641 cggcgaggcc gcccgtcacc gcttcgccga cgctttccag ccccccgctc atcatcgcgg
    17701 caacatgacc gaggcggagc ggcgcgtcga gacgctttgc aagggcgaca cgagaaggcg
    17761 aagcgcgagc aaaaggttca tggcgttcaa tacggcgacc gttccggcga ggtatgaaca
    17821 agtgccggtc aagttcggcc ggcgtcgaag agcagaaagg ctatcgaaat gaccaagccg
    17881 ctcaaagaac tggtcgcgag cacgggagac cagggtctca aggtgttcgt ccgcaccgcc
    17941 tcccgcgagc tgacgctgga cgacctcgag gtcatcgccg gcggccgcgg cgcccggtgg
    18001 gatccgtacg gctgatgccg ccgccgccgg aggagcgtgc gagccgttcc gcgaccccct
    18061 cgcgccccag gccgcatgct cttcgccggc gggcggggct ccgagtccgg cactgcgccg
    18121 cgccgccgga tgccgccgcc cgttcaacct gttgaaggac cggtccgatg caattttcgc
    18181 ctggcgaccc gtttccctat ctggaagggc agacgc
//
LOCUS       DASVQZ010000076         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_114646, whole genome shotgun sequence.
ACCESSION   DASVQZ010000076 DASVQZ010000000
VERSION     DASVQZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114646"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VF547_02145"
     CDS             complement(<1..798)
                     /locus_tag="VF547_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8261654.1"
                     /translation="MSLLRALLLLLVLMASPAAAQEFPALTGRVVDAANIIPDADEAA
                     LTQELKALERNSSRQLVVATVPSLGGYPIEDYGYQLGRHWGIGQKGANNGVILLVAPN
                     ERKVRIEVGYGLEPIMTDALSNQIITQQILPRFKADDYPGGIRAGAAEIVAQLQAPPE
                     QAEQKALEAQQAAESRGSGEDDFGGLFPLIFWGAVLLFVFLRMIGRGGRRGRRHRRRG
                     VWVWGPGVGGGDWGGGWGGSSSGGGFDFGGGGFSGGGGSFGGGGASGG"
     gene            complement(795..1349)
                     /locus_tag="VF547_02150"
     CDS             complement(795..1349)
                     /locus_tag="VF547_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX8261655.1"
                     /translation="MRQALILPLLLLGAAACDRQPAPAPQAAAAGGETLPALTGPVVD
                     RADLLSPDEEARLARKAEALRREIGPQYVIVTVASLGGRPIEDYSLDLARRWGLGHKD
                     RDDGLMLLVAPAERKVRIEVGVGLERRVTNGYAAQVIEERALPSFKAGRFPQGIEEAS
                     DALIARLRAEPDGADKPREKPVAL"
     gene            complement(1358..1969)
                     /locus_tag="VF547_02155"
     CDS             complement(1358..1969)
                     /locus_tag="VF547_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8261656.1"
                     /translation="MKRTSLMMAFLAPVAGVSLAGCGVNSIPTAEEEAKAKWADVQAA
                     YQRRANLIPNLVSTVRGAAASEERILVGVTEARANATRITVSPDQLTDPAAMQRFSEA
                     QSRLSAAILPLQRLQEAYPNLQSQANFATLMSQLEGTENRINIAIRDYNSAVQSYNTE
                     IRTFPSVIGAKVIYGAKPMATYQATTPNADVAPEVNFDTNTSR"
     gene            2044..2421
                     /gene="ssrA"
                     /locus_tag="VF547_02160"
     tmRNA           2044..2421
                     /gene="ssrA"
                     /locus_tag="VF547_02160"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            2561..2806
                     /locus_tag="VF547_02165"
     CDS             2561..2806
                     /locus_tag="VF547_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261657.1"
                     /translation="MRALLLAPLLLLAACEPEPENIQAKSETMERLLESRAKEIEAEA
                     ENGVDAQVAPLDNEADALLNQLESNAAAPANAAANAQ"
BASE COUNT          455 a         1055 c          984 g          427 t
ORIGIN      
        1 tcctcccgac gcgccgccgc cgccgaacga gccgccgccg cccgagaagc cgcctccgcc
       61 gaagtcgaag ccgccgccgc tcgagcttcc gccccagccg ccgccccagt cgccgccgcc
      121 gacgcccggc ccccagaccc agactccgcg ccgccggtgc cgccgcccgc gccggccgcc
      181 gcggccgatc atcctgagga agacgaacag cagcaccgcg ccccagaaga tgagcgggaa
      241 caggccgccg aaatcgtcct cgcccgagcc gcgcgattcg gccgcctgct gcgcctcgag
      301 cgccttctgc tcggcctgct cgggcggcgc ctggagctgg gcgacgatct cggcggcgcc
      361 ggcgcggatg ccgccgggat aatcgtccgc cttgaagcgc ggcaggatct gctgcgtgat
      421 gatctggttc gacagcgcgt cggtcatgat cggctcgagc ccgtagccga cctcgatccg
      481 caccttgcgc tcgttggggg cgacgaggag gatgacgccg ttattggccc ccttctggcc
      541 gatcccccaa tggcggccga gctggtagcc atagtcctcg atcggatagc cgccgaggct
      601 gggcacggtg gcaacgacga gctggcggct gctgttgcgc tcgagcgcct tcagctcctg
      661 ggtcagcgcc gcctcgtccg cgtcggggat gatgttggcg gcgtcgacga cccggccggt
      721 cagcgccggg aactcctggg cggccgccgg cgacgccatc agcaccagca ggagaagcag
      781 ggcgcgcagc aggctcatag cgcgaccggc ttttcgcgcg gcttgtcggc gccgtcgggc
      841 tcggcgcgga gccgggcgat gagcgcgtcg ctcgcctctt cgattccctg cgggaagcgg
      901 cccgccttga agctgggcag cgcacgctcc tcgatcacct gcgccgcata gccgttggtc
      961 acccgccgct cgagcccgac cccgacctcg atccgcacct tgcgctcggc cggggcgacc
     1021 agcagcatca gcccgtcgtc gcggtccttg tggcccaggc cccagcgccg cgccagatcg
     1081 agcgaataat cctcgatcgg ccggccgccg agcgaggcga cggtgacgat cacatattgc
     1141 ggcccgatct ccctgcgcag cgcctcggcc ttgcgggcga gccgcgcttc ctcgtccggc
     1201 gacagcaggt cggcccgatc gacgaccggt ccggtcaggg ccggaagcgt ctcgcctccg
     1261 gcggcggccg cctgcggcgc cggcgcgggc tgccggtcgc aggcggccgc gccgagcagc
     1321 agcagcggaa ggatcagcgc ctggcgcacc gtccgcgtca gcggctggtg ttggtgtcga
     1381 aattcacctc cggcgccacg tccgcgttcg gagtggtcgc ctggtaggtc gccatcggct
     1441 tggccccgta gatcaccttc gcgccgatca ccgaggggaa ggtgcggatc tccgtattat
     1501 aggactgaac ggcgctgtta tagtcgcgga tggcgatgtt gatccggttc tcagtgccct
     1561 cgagctggct catcagggtg gcgaaattgg cctggctctg caggttcgga taggcttcct
     1621 ggagccgctg cagcggcagg atggcggcgc tcagccggct ctgcgcctcg gagaaacgct
     1681 gcattgcggc cgggtcggtc agctggtccg gcgagaccgt gatccgggtg gcgttggcgc
     1741 gcgcctcggt cacgcccacc aggatccgct cctcggaggc ggcggccccc cgcacggtcg
     1801 agacgaggtt cgggatcaaa ttcgcccggc gctggtaggc ggcctggacg tcggcccatt
     1861 tggccttggc ctcttcctcg gcggtcggga tcgaattgac tccgcatccg gcgagcgaga
     1921 ccccagccac cggcgccagg aaagccatca tcagggaagt gcgcttcatg tcggacccct
     1981 cgttcaactg cgagcaagca agtagggact tcgcttcgcc cccgcaatgt ctcgcgcccg
     2041 ggacggcttt aaattaaccg cgaagcgcct atatggggag gtgccgggct tgcccggcta
     2101 tggcgataaa ctgcggcgtg caatagacgc agcggacccg ggggcggtac ccggcggctc
     2161 caccaggtgg gccgagccac aagggcgaca cgcgtggacc gcgcgccgcc cctgcggctc
     2221 agctgatggg gccgaactag gatcgacgtg tgttgaaaag cgctgttttt gcccggcctg
     2281 aatgcgccgt aaaaccgttc aaaaccacaa gtgccaacga caacgaggcg ctcgcgattg
     2341 ccgcgtaaca aggtcctcac ggactgagtt acaaggacga aagcgcggtt cgggccgcac
     2401 cgggcaacag aagcggacac cggcggtacg gggagcaccg agcaacagaa gctccccact
     2461 tccttaccgt cgcccggcga aggccgcctc ccgcgggtga aagacaccgc gcttttaccg
     2521 ccgggtttcc tgcgttcgcg ggaatgacgt atgaggcgtc atgcgcgccc ttctcctcgc
     2581 cccgcttctc ctcctcgccg cctgcgagcc cgagcccgag aacatccagg ccaagtccga
     2641 gaccatggag cgcctgctcg aatcgcgggc gaaggagatc gaggccgagg cggagaacgg
     2701 cgtcgacgcc caggtcgccc ccctcgacaa cgaggccgat gccctcctca accagctcga
     2761 atcgaacgcc gccgcgccgg cgaacgccgc cgccaacgcg caatagacgc gccgcccccc
     2821 gccccccgcg gcccgcaatc ttgccgccgc cggccggacc tcattgcgga cccgaaagca
     2881 gcggttcagc atgagcggtg cagacgcccg aaatatgaat c
//
LOCUS       DASVQZ010000077         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_402544, whole genome shotgun sequence.
ACCESSION   DASVQZ010000077 DASVQZ010000000
VERSION     DASVQZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402544"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF547_02170"
     CDS             <1..188
                     /locus_tag="VF547_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C13"
                     /protein_id="HEX8261658.1"
                     /translation="QPLAAASTEALKLVGEWEADKKLDPSYPQVSIGSRVDAWLKPLE
                     ARMPKTATRPVGTPAVD"
     gene            complement(319..1173)
                     /locus_tag="VF547_02175"
     CDS             complement(319..1173)
                     /locus_tag="VF547_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261659.1"
                     /translation="MTRFERFAAIDWSGAKGRRHKGIAVAVAEAGTASPRLIRQGHAW
                     ARTELLEWLLAEAAAAPTLFGFDFSFAPPIAERGEYLPGEPDVPSTAREFWAYVDERS
                     DDEDLGAASFLEQVHRRHFYFGIADGVKARFMHFRQCDARLNLQGGRKTASAYDAIGA
                     AQVAKASFSGMRLLHRLDGMVAIWPMDELPEQGSAVVEIYTRIYLRRAGLSGTKLRSR
                     ADLDRALVGLGSRPARLRFEPDDHQTDAMVTAAGMRALAADEPRAFDPEGLTPELARS
                     EGWTFGIL"
     gene            complement(1316..2629)
                     /locus_tag="VF547_02180"
     CDS             complement(1316..2629)
                     /locus_tag="VF547_02180"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEX8261660.1"
                     /translation="MYSNEAGDADYWQAQLMFWGPRILAAIAILVIAWLLARAAKWAI
                     AKVVDRVPALRRHYEAEPGRTLGSLIGDIAFWLIILIGIMLALQPLQLGGVLDPVRQL
                     TTNALAFIPNVVGAGLIFFIGLIVARIVRRLVEGSLLAANADGWLRKTGLAETAGTVP
                     ASPEGAAPSVAASRTSISRSVGMVVFFLIMIPVTISALDALKIQAISGPATEMLRTIL
                     DSIPLVIGALILLAIGYLLGKLAKQAIEQLLPSMGFDRSVSAIGIAPAGGSPSRTVGT
                     AVMIAILIFFAIKAAELLQSPLIAAMLAQVLELGSRVLFGAVIILGGVAIARIVSNLV
                     SGAATEGWLPTILRWSIIALAVAMGLTFMGLANEIVIIAFAAIIGSAAVACALAFGLG
                     GRPTAHKLLERWTEGNAVPATPSRPRPPKAPTPPTPPSDSQPPLV"
     gene            complement(2703..>3179)
                     /locus_tag="VF547_02185"
     CDS             complement(2703..>3179)
                     /locus_tag="VF547_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8261661.1"
                     /translation="RGLRARLGDGERALAFPFESLLWKIGMTRTFPETDWQGNFVLSS
                     QVWTTARDLARLGLLYQNDGVWNGERILPAGWGAYVSRRGPAQPAGGYGYGATFWTFP
                     ADLGLPADAYVGQGNRGQYLAIVPSRRIVIVRRGYDGPGTGFDPGAFVRDVLAALR"
BASE COUNT          501 a         1090 c         1156 g          432 t
ORIGIN      
        1 cgcagccgct ggccgccgcg tcgaccgagg cgctgaagct ggtcggcgag tgggaggcgg
       61 acaagaagct ggacccctcc tacccccagg tcagcatcgg cagccgggtc gacgcctggc
      121 tgaagccgct cgaggcgcgg atgccgaaga ccgcgacccg gccggtgggg acgccggcag
      181 tggactgaaa cccctctccc tgaaggggag agggacaggc gcgaagcgcc agggtgaggg
      241 cctggaatgg ggtcggaatg ccctcaccct cccaccgctc cgcggcgggc ccctccctct
      301 cccctgcagg gagagggatt ataaaatccc gaacgtccaa ccctcgcttc gggcaagctc
      361 gggggtcaga ccttccgggt cgaacgctcg cggctcgtcg gccgccagag cccgcattcc
      421 ggccgccgtg accatggcgt cggtctggtg gtcgtccggc tcgaagcgaa gccgcgccgg
      481 acggcttccg agccccacga gagcgcggtc gagatcggcc cgcgaccgaa gcttggttcc
      541 gctcaggccg gcccggcgca gatagatccg ggtgtagatc tcgacgacgg cgcttccctg
      601 ctccggcagc tcgtccatcg gccagatcgc gaccatgccg tcgaggcggt ggagcagccg
      661 catgccggag aagctggcct tggcgacctg ggcggcgccg atcgcatcat aggcgctggc
      721 cgtcttgcgg ccgccctgga ggttcagccg ggcatcgcac tgccggaaat gcatgaatcg
      781 cgccttcacg ccgtccgcga tcccgaaata gaaatggcgg cggtggacct gctcgaggaa
      841 gctcgccgcg cccaggtcct cgtcgtcgct tcgctcgtcg acataggccc agaattcgcg
      901 cgcggtcgac gggacgtccg gctcgcccgg cagatattcg ccgcgctcgg cgatcggcgg
      961 ggcgaagctg aagtcgaagc cgaacagggt cggcgccgcg gccgcctcgg cgagcagcca
     1021 ttccagcagc tcggtgcgcg cccaggcatg gccctggcgg atcagccgcg ggctcgccgt
     1081 ccccgcctcg gcgacggcga cggcgatgcc cttgtggcgc ctgcccttcg cgcccgacca
     1141 gtcgatcgcg gcgaagcgct cgaaccgggt caccgctcag tcctccccgt cgcgcagcga
     1201 tggggagggg gcccatgcga agcgtggtgg aggagccgat ggcgcggtcg gcccctccac
     1261 cacccttcgg gcggtcccct ccccacgagc ttcgctcgca gggaggacga ggtgctcaca
     1321 ccaggggcgg ctggctgtcc gacggcggag tcggcggcgt cggcgctttc ggcggcctcg
     1381 gccgcgaggg tgtcgccggc accgcattgc cctccgtcca gcgctcgagc agcttgtgcg
     1441 ccgtcggccg tccgcccagc ccgaaggcga gagcgcaggc gacggccgcc gagccgatga
     1501 tcgcggcgaa ggcgatgatc acgatctcgt tggcgaggcc catgaaggtc aagcccatcg
     1561 cgaccgccag ggcgatgatc gaccacctga ggatggtcgg cagccagccc tcggtggcgg
     1621 cgccgctgac caggttcgag acgatccgcg cgatcgccac gccgccgagg atgatcaccg
     1681 cgccgaacag cacgcggctg ccgagctcga gcacctgggc gagcatcgcg gcgatcagcg
     1741 gcgactggag cagctcggcg gccttgatcg cgaagaagat gaggatcgcg atcatcaccg
     1801 cggtgccgac ggtgcgcgac ggcgagcctc ccgccggggc gatgccgatc gcgctgaccg
     1861 accggtcgaa gcccatcgac ggcagcaatt gctcgatcgc ctgcttggcg agcttgccga
     1921 gcagatagcc gatggcgagc aggatcagcg ccccgatcac cagcgggatc gaatcgagga
     1981 tggttcggag catctcggtc gccgggccgg agatggcctg gatcttgagc gcgtcgagcg
     2041 ccgagatggt caccgggatc atgatcagga agaagacgac catgcccacc gagcggctga
     2101 tcgacgttcg gctcgcggcg acgctgggag cggcgccctc ggggctcgcc gggaccgtgc
     2161 cggcggtctc ggcgaggccg gtcttgcgca gccatccgtc ggcattggcg gcgagcagcg
     2221 agccttcgac cagccggcgg acgattcggg cgacgatcag gccgatgaag aagatcagcc
     2281 cggcgccgac gacgttcggg atgaaggcga gcgcgttggt ggtgagctgc cgcaccggat
     2341 cgagcactcc gccgagctgg agcggctgca aggcgagcat gatgccgatg aggatgatga
     2401 gccagaaggc gatgtcgccg atcaggctgc cgagcgtccg gcccggctcg gcctcatagt
     2461 gccgccgaag cgccggcacc cggtcgacga ccttggctat cgcccatttg gcggctcgcg
     2521 cgagcagcca ggcgatgacc agaatggcga tggcggcgag tatcctgggc ccccagaaca
     2581 tgagctgcgc ctgccagtaa tcggcgtctc cggcctcgtt gctgtacata ggaatgtccc
     2641 cttgttccgg gcggccgcgc cgctccgctt caataatgag cgggcggcgg ccggggttcc
     2701 gctcagcgca gtgcggcgag cacgtcccgg acgaaggcgc ccgggtcgaa gccggtgccc
     2761 ggcccgtcat agccgcggcg gacgatcacg atccggcgcg acgggacgat tgccagatac
     2821 tggccgcggt tgccctgccc gacataggcg tcggcgggca ggccgagatc cgccgggaag
     2881 gtccagaagg tggcgccgta gccgtatccg ccggccggct gggcgggccc gcgccgcgag
     2941 acgtaggcgc cccagcccgc cggaaggatg cgctcgccgt tccagactcc gtcgttctgg
     3001 tagaggaggc cgagccgggc gaggtcgcgc gccgtcgtcc agacctggct ggagaggacg
     3061 aaattgccct gccagtcggt ctccgggaag gtccgggtca tgccgatctt ccacagcagc
     3121 gactcaaacg ggaaggcgag cgcccgctcg ccgtcgccga gcctggctcg aaggccgcg
//
LOCUS       DASVQZ010000078         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_306046, whole genome shotgun sequence.
ACCESSION   DASVQZ010000078 DASVQZ010000000
VERSION     DASVQZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306046"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..613
                     /locus_tag="VF547_02190"
     CDS             68..613
                     /locus_tag="VF547_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8261662.1"
                     /translation="MFTDMRNGTAQDWAHIAAEHGKHIRTTAARQIMEGLARLEAIEV
                     GFAANQLTHSLMAGTLARRSGASDEEVVAALCHDLGKLFSIPTHGPIAAEMLKPYVRE
                     DIYHAVYWHQEFQGRYYFEHLGKDPEGRERFKGESWYGFAEKLVDDWDAPAFDPGFEV
                     DPLESFEPEVTRVFSNPRSMV"
     gene            736..1764
                     /locus_tag="VF547_02195"
     CDS             736..1764
                     /locus_tag="VF547_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8261663.1"
                     /translation="MAIQPVRWPALKDWRDVLVRTWKEAGDDNVGLLAAGVAFYAFLA
                     FVPLLAATVLVYGLAAEPETVAGHIRVLFSLLPRDAAILVGDQLKSMTESPESAKGWA
                     LVAAIALAIYGASKGSGAIVTALNIAYEVKESRGFVKSTLLSLAMTVGALVVLVAAAA
                     GISVIGYVEAFFPGFPGWAHKAFQFLFWAVAVVAVGFGLAAVYRYAPNRPDAPWVWIS
                     PGSASATLLWLAASLGFGLYVAQFGNYNATYGSLGGVVVFLTWLYLSAYIVLMGGEMN
                     SELERQQAKEAGLPARPAAAAAAAARAEAPPAPAERRPDRVSWPLAALGFLLSRRAPG
                     GPARPPSR"
     gene            complement(1685..>2346)
                     /locus_tag="VF547_02200"
     CDS             complement(1685..>2346)
                     /locus_tag="VF547_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8261664.1"
                     /translation="AALLACAGSGARRRRPGARAGIGFALLLFALIAWQLTDFLDQSY
                     YVALALVLVVLVGEQLLVLRQARSAGEKEALRAAGLEARLKEAAAGSGDRIARLKDGA
                     VTRQVPEAQILFVRAADDYCEARLADGRTLLVTMSLSRLQQALSERFVRIHKSYSVNA
                     AQVAALGPRPGGGRQLTLADGTILPVGRAYMDAVSAMEAEPGRPAPGARGGSRGPQGA
                     S"
BASE COUNT          332 a          841 c          816 g          357 t
ORIGIN      
        1 gccggggccg cttttcgttg aaagcgccgg cggcggggcg taatccggcg gcgacaggag
       61 agcgctgatg ttcaccgaca tgaggaacgg caccgcccag gactgggcgc atatcgcggc
      121 cgagcacggc aagcacataa ggacgaccgc cgcgcgccag atcatggagg gcctcgcccg
      181 actggaggcg atcgaggtcg gattcgccgc caaccagctc acccacagcc tgatggccgg
      241 gaccctcgcc cgccgctcgg gcgcctcgga cgaggaagtg gtcgcggcgc tctgccacga
      301 cctcggcaag ctcttctcga tccccactca cgggccgatc gccgccgaga tgctcaagcc
      361 ctatgtccgc gaggacatct accacgccgt ctactggcac caggaattcc agggccgcta
      421 ttatttcgag catctcggca aggaccccga ggggcgcgag cgcttcaagg gcgagagctg
      481 gtacggcttc gccgagaagc tggtcgacga ctgggacgcg ccggcgttcg atccgggctt
      541 cgaggtcgac ccgctcgaga gcttcgagcc ggaggtgacg cgggtgttca gcaacccgag
      601 gtcgatggtc tgattcgtct ccccagcaaa ggctggggcc ccggagagga aagacgccgc
      661 tgcctttcac ccgcttggac cccagccttc gctggggtga cggaaccgcg gccgctccct
      721 ccggttctcg ccgccatggc aatccaaccc gtccgctggc ccgcgctcaa ggattggcgg
      781 gacgtactcg tccgcacctg gaaggaggcg ggcgacgaca atgtcgggct gctcgccgcc
      841 ggggtcgcct tctacgcctt cctcgccttc gtgcccctgc tggcggcgac ggtgctggtc
      901 tacggcctcg ccgccgagcc ggagacggtc gcggggcata tccgcgtttt gtttagtttg
      961 ttgccgcgcg acgccgcaat tctcgtcggc gaccagctga aaagcatgac ggaatcgcca
     1021 gaaagcgcca aaggctgggc cctggtcgcc gccatcgcgc ttgcgatcta cggcgccagc
     1081 aaaggctccg gcgcgatcgt caccgcgctc aacatcgcct atgaggtcaa ggagtcgcgc
     1141 ggcttcgtca agtcgaccct cctctcgctc gccatgaccg tcggcgccct cgtcgtgctg
     1201 gtcgccgccg ccgcgggcat ctcggtgatc ggctatgtcg aagccttctt ccccggcttt
     1261 cccggctggg ctcacaaggc gttccagttc ctgttctggg ccgttgcggt ggtcgcggtc
     1321 ggcttcggcc tcgccgcggt ctaccgctac gcgcccaacc ggccggacgc gccctgggtc
     1381 tggatcagcc ccggctcggc ctcggcgacg ctgctgtggc tggccgccag cctcggcttc
     1441 ggcctctacg ttgcccagtt cggcaattac aacgcgacct acggctcgct cggaggggtc
     1501 gtcgtcttcc tcacctggct ctacctttcc gcctatatcg tcctgatggg cggcgagatg
     1561 aactcagagc tggagcgcca gcaggcgaag gaggcgggcc tcccggcgcg gcccgcggcg
     1621 gcggcggcgg cggcggcgag ggccgaggcc ccgccggccc cggccgagcg gcggcccgac
     1681 cgggtcagct ggccccttgc ggccctcggc ttcctcctct cgcgccgggc gccgggcggc
     1741 ccggctcggc ctccatcgcg ctgacggcgt ccatataggc gcgtccgacc ggcaggatcg
     1801 tcccgtcggc gagggtgagc tgccggccgc cgccggggcg cggcccgagc gcggccacct
     1861 gggcggcgtt gaccgaatag cttttgtgga tgcgcacgaa ccgctccgac agcgcctgct
     1921 ggaggcgcga caggctcatg gtgacgagca gggtgcggcc gtccgcaagc cgggcctcgc
     1981 aataatcgtc ggcggcgcgg acgaacagga tctgcgcctc cggcacctgg cgggtgaccg
     2041 cgccgtcctt cagccgcgcg atccggtcgc cggagccggc cgcggcctcc ttcagccgcg
     2101 cctcgaggcc ggccgcgcga agcgcctcct tctcgccggc gctgcgcgcc tgacgcagga
     2161 ccagcagctg ctcgccgacc agcacgacca ggaccagggc gagcgcgaca taataggact
     2221 ggtcgaggaa atcggtcagc tgccaggcga tcagggcgaa gaggagcagg gcgaatccga
     2281 ttcccgcgcg ggctccgggc ctgcggcgcc gggcgccgct gccggcgcag gcgaggaggg
     2341 ccgccg
//
LOCUS       DASVQZ010000079         4708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_593710, whole genome shotgun sequence.
ACCESSION   DASVQZ010000079 DASVQZ010000000
VERSION     DASVQZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593710"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1681
                     /locus_tag="VF547_02205"
     CDS             167..1681
                     /locus_tag="VF547_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8261665.1"
                     /translation="MTEHSIRDIVIVGGGTAGWMAAAALSHLLDRRTVRINLVESDEI
                     GTVGVGEATIPPLIAYNNMLGINEDEFVAATKGTFKLGIEFVDWGLPGERYFHPFGDH
                     GQDFRGVHFHQLYLRERRRRALDDISHWSMSAAAARLGRYARPGPDARLPFSQLLYAF
                     HFDAGRYARFLRGHAERNGVRRTEGKIVDTTLDSVNGHVRSVTLADGSILAGDLFIDC
                     SGFRGLLIEQVLGTGYEEWTHWLPCDRAVAVPSTYPGQPDPFTRSTARPGGWQWRIPL
                     QHRMGNGLVYSSAHLADADAEALLLANLEGEKLADPRRLSFTAGRRRLAWNRNVVSLG
                     LSSGFIEPLESTSIHLVQSGIVKLLALFPDRRFDPVERDEYNRQMQDVYEDIRDFIIL
                     HYKATRRDDSDFWNYCRTMEVPDSLAWKMELWRAKGRLFREGRELFGTPSWVAVMLGQ
                     GIVPEDHEPAADALDPDMIADALDKMRTSYRQMAEHMPTHGDFLARSAAAVRTA"
     gene            complement(1668..3143)
                     /locus_tag="VF547_02210"
     CDS             complement(1668..3143)
                     /locus_tag="VF547_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8261666.1"
                     /translation="MSGEQEGGRLIVRGAQATALGFAARLAARLIFLFIAGRLFGASA
                     FGAFALALAVVELAVAVGSLGTKKTLFHLLDRHGGESRRPLPHLLLDAALLVGAASLM
                     LGIAIGAAALAMPASLLGPAVATALLWLAPTVAGQALLDLLLAATRWTHAIRHEVVAR
                     SVIEPWAMLAGTAGAWLLGWRAEGLAIGYGCGTLAALIYAASAARRRLGGLRLPAWRP
                     NRRALAETVRGSADNTATDLLNVLYNRVDLYLVGALLGEAPAGIYGMARQLAAPLRQI
                     RQSFDGLLIPLAARSLAAGGVRGAAEALASATRLVLVLQLPLLLAFFAAGRPFLAWIG
                     ADFAAAYWALVALAAAETLQAALSVGDLVFVYLRPRVGLWLTLGSIVVGIAAALCLIP
                     ALGTAGAALSVLLAYGVRAAIRSWVLRASFGFDVPRAHNAAPLAASACGAAAAWLIGG
                     GWWALAASVALYAVVLASWLKATGERLALSGFAVPAEVRPS"
     gene            3208..4335
                     /locus_tag="VF547_02215"
     CDS             3208..4335
                     /locus_tag="VF547_02215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8261667.1"
                     /translation="MRAPPPDARPAVAVFRAPLFNPSETFVRAHLAALRRYRPLAVGL
                     VAKGETDARRLLPASARERLALTLFGRAEALAGRLRPENPALVHAHFGTDGLLALPLA
                     EALGVPLVTTLHGHEVSRAPARLLLSGRLTWMRYALVGRRLRERGRLFLAVSDAVRKR
                     ALDAGYPPERTIIHYNGVDTSVFRPSGAAPEPGLILHVGRLVEKKGTRDLIDAVAGID
                     GAILVVVGDGPSRRALERRARALGAAVRFAGALPPVEVLGWMRRAWLLAAPSVTARDG
                     DAEGLPTVICEAAAAGLPVVATRHSGIPEAVLDGETGLLSAEGDVAALARNLSFLLAS
                     PDMREAMSAAGRRLARERFDLEAQTARLERHYDSVVGRSSA"
     gene            4335..>4708
                     /locus_tag="VF547_02220"
     CDS             4335..>4708
                     /locus_tag="VF547_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261668.1"
                     /translation="MRLVYPVLWSRLGREASQEQTANTVAALARHGVEITLVLPRGAV
                     DPPLDAEAVRLWFGVRGDFGLVQRRSRWGGENAVRSTLWMREMLRDPVLAGADLLYSR
                     VPVMLAWPGPYPLPFATDLYRP"
BASE COUNT          697 a         1721 c         1645 g          645 t
ORIGIN      
        1 tattcaagcg gttgaacctg acaagccatt tgtccgattt atagaacagt gtggctggac
       61 ggacgcagcc gccagggcgc gagaaggcga tccggccgag cccggccgcg caggagttga
      121 ggttgcccac cttgcggatc ggactggcct gccgcactgg aagccgatga ccgagcattc
      181 gatccgcgac atcgtcatcg tcggcggcgg caccgcaggg tggatggccg cggccgccct
      241 ctcgcacctc ctcgaccgaa ggacggtccg gataaacctg gtcgagtcgg acgagatcgg
      301 cacggtcggg gttggcgagg cgaccatccc gccgctcatc gcctacaaca acatgctggg
      361 gatcaacgag gacgaatttg tcgccgcgac caagggtacg ttcaagctgg gcatcgagtt
      421 cgtcgactgg ggcttgcccg gcgagcgcta cttccatccg ttcggggacc atggccagga
      481 cttccgcgga gtccatttcc accagctcta cctgcgcgaa cgaaggcgac gggcgctcga
      541 cgatatctcg cactggtcga tgagcgcggc cgccgcccgg ctcggacgct acgcccggcc
      601 cggaccggac gcccgccttc ctttctccca gctcctctac gccttccatt tcgacgccgg
      661 ccgctatgcc cgcttcctgc gcggccatgc cgagcgcaac ggcgtgcgcc gaaccgaggg
      721 gaagatcgtc gacaccaccc tcgactccgt gaacgggcat gtccggtcgg tcaccctggc
      781 cgacggctcg atcctcgccg gcgacctgtt catcgactgc tcgggcttcc gcggcctgct
      841 gatcgagcag gtgctcggca ccggctacga ggagtggacg cactggctgc cctgcgatcg
      901 ggcggttgcg gtgccgagca cctatccggg ccagcccgac cctttcaccc gatcgaccgc
      961 ccgtccaggc ggctggcaat ggcggattcc gcttcagcac cggatgggca acggccttgt
     1021 ctattcgagc gcccacctgg ccgacgccga cgccgaggcg ctgctgcttg cgaacctcga
     1081 gggcgagaag ctcgccgacc cgcgccgcct gtccttcacg gccggccgcc ggcgcctggc
     1141 gtggaaccgc aacgtcgtct cgctcggcct ttccagcggc ttcatcgagc cgctcgaatc
     1201 gaccagcatc cacctggtcc agagcggcat cgtcaagctg ctggcgctct ttcccgaccg
     1261 ccggttcgat ccggtcgagc gcgacgaata taatcggcag atgcaggacg tctacgagga
     1321 catccgcgac ttcatcatcc tccattacaa ggcgacgcga cgcgacgatt ccgacttctg
     1381 gaactattgc cggacgatgg aggtaccgga cagcctggcc tggaagatgg agctttggcg
     1441 ggcgaagggc cgtctgttcc gcgaggggcg cgagctgttc ggcacgccca gctgggtcgc
     1501 ggtcatgctc ggccagggca tcgtccccga ggaccacgag ccggccgccg acgcgctcga
     1561 ccccgacatg atcgccgacg cgctcgacaa gatgcgcacc agctatcggc agatggcgga
     1621 gcacatgccg acgcacggcg acttcctcgc ccgctcggcc gccgctgtca ggacggcctg
     1681 acctcggcgg gcacggcgaa gcccgacagg gccagccgct cccccgtcgc cttcagccag
     1741 gacgcgagaa cgaccgcgta gagggccacc gaggcggcga gcgcccacca gccgccgccg
     1801 atcagccagg ccgccgccgc cccgcaggcg gacgcggcga gcggcgcggc attgtgggcg
     1861 cggggcacgt cgaagccaaa gctcgccctg agcacccagc tgcggatcgc ggcccgcacg
     1921 ccataggcca aaagcaccga aagtgccgct ccggccgtgc cgagagcggg aatgaggcag
     1981 agggccgccg cgatcccgac gacgatgctc ccgagggtca gccagagccc gaccctcggc
     2041 cgcagataga cgaagacgag atcgccgacg ctcaaggcgg cctgcagggt ctcggcggcg
     2101 gcgagggcca ccagcgccca ataggcggcg gcgaaatccg ccccgatcca ggcgaggaag
     2161 ggccggccgg cggcgaagaa ggcgagcagc agcggaagct gcaggacgag cacgagccgg
     2221 gtcgcggagg cgagcgcctc ggcggctccc ctcaccccgc cggccgcgag gcttcgcgcc
     2281 gcgagcggga tgagcaggcc gtcgaagctc tggcggatct ggcgaagcgg cgccgcgagc
     2341 tgccgcgcca tgccgtaaat gcccgccggc gcctcgccga gcagcgctcc gaccagatag
     2401 aggtcgacgc ggttgtagag cacgttgagc aggtcggtgg cggtgttgtc ggccgatccg
     2461 cgcacggttt cggccagagc ccgccggttg ggccgccagg cggggaggcg cagcccgccg
     2521 agccgccgcc gagccgccga ggcggcgtag atgagcgcgg caagcgtgcc gcacccatag
     2581 ccgatggcga gaccctccgc gcgccagccc agcagccagg ccccggcggt gccggccagc
     2641 atcgcccagg gctcgatcac gcttcgggcg accacttcgt ggcggatggc gtgggtccag
     2701 cgggtggcgg cgagcagcag gtcgagcagc gcctggccgg cgaccgtcgg cgcgagccag
     2761 agcagggcgg tggcgaccgc cgggccgagc agcgaggcgg gcatggccag ggcggctgca
     2821 ccgatcgcga tcccgagcat gaggctggcg gcgcccacca gaagcgccgc gtccaggagt
     2881 aggtgcggca acgggcggcg cgattcgccg ccgtgccgat cgagcaaatg gaacagggtc
     2941 ttcttggtgc cgaggctgcc gacggccacc gcgagctcga ccaccgccag agccaaagcg
     3001 aaggcgccga aggcgctggc gccgaacagg cgtccggcaa tgaacaggaa gatcagcctc
     3061 gccgcgagcc gggcggcaaa gccgagcgca gtcgcctgcg cgccccggac gatcagccgg
     3121 ccgccttcct gctctccgct catgccgcgc gcgccgttcc cactttcggc ttgtatcccg
     3181 gctcgcgacc gatgaccaag gggggcaatg cgcgctcccc cgcccgacgc ccggcccgcg
     3241 gtcgcggtct tccgcgcgcc gctcttcaac ccgtcggaaa ccttcgtccg ggcgcatctg
     3301 gcggccctgc ggcgctaccg gccgctcgcc gtcgggctcg ttgccaaggg ggagacggac
     3361 gcgcgccggt tgttgccggc aagcgcgcgc gagcggcttg ccctgaccct gttcggacgg
     3421 gccgaagccc ttgccgggag gctccgaccc gaaaacccgg cgctggtcca cgcccatttc
     3481 ggcaccgacg gcctgctcgc cttgccgctc gcggaggcgc tcggcgtgcc gctcgtcacc
     3541 acgctccatg gccatgaagt gagccgcgcg ccggctcggc tgctgctttc gggccgactg
     3601 acctggatgc gctatgccct ggtcggacgg cggctgcgcg agcgcgggcg gctcttcctc
     3661 gccgtctccg acgcggtgcg gaaacgcgcc ctcgatgcgg gctatccgcc ggagcgcacg
     3721 ataatccact acaacggcgt cgacacgagc gtcttccggc cgtccggagc ggcgccggag
     3781 cctgggctca tcctccatgt cggccgcctg gtggagaaga aagggacgcg cgacctgatc
     3841 gacgccgtgg ccggcatcga cggcgccatc cttgtcgtcg tcggcgacgg accgtcgcgg
     3901 cgcgcgctgg agcggcgggc gcgcgcgctc ggagcggccg tgcgcttcgc cggggcgctg
     3961 ccgccggtcg aggtgctcgg ctggatgcgg cgcgcatggc tgctggcggc gccgagcgtc
     4021 accgcccggg acggcgacgc cgaggggctg ccgaccgtca tctgcgaggc cgccgccgcc
     4081 ggactgcccg tcgtcgccac ccgccactcg ggcattcccg aggcggtcct cgacggcgag
     4141 accggcctgc tctcggccga aggggacgtc gccgccctcg cccgcaacct ttcgttcctg
     4201 ctcgcctccc ccgacatgcg cgaggccatg tccgcagccg gacgccggct cgcccgcgag
     4261 cgcttcgacc tcgaggctca aaccgcccgc ctcgagcggc attacgattc ggtcgtcggc
     4321 cggagctcgg cctgatgcgg ctcgtctacc ccgtcttgtg gtcgcgcctc ggtcgcgagg
     4381 ccagccagga gcagacggcg aacacggtcg ccgcgctggc ccggcacggc gtggaaatca
     4441 ccttggtcct gccccgcgga gccgtcgatc cccccctcga cgccgaggcg gtgcgcctat
     4501 ggttcggagt gcggggcgat ttcggcctcg tccagcgccg gagccgctgg ggcggcgaga
     4561 acgcggtccg ctcgaccctt tggatgcgcg agatgctccg cgatccggtg cttgccggcg
     4621 ccgatctcct ctattcgcgg gtgccggtca tgctggcctg gccggggccc taccccctcc
     4681 ccttcgcgac cgacctctat cgtccctg
//
LOCUS       DASVQZ010000080         4899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_115415, whole genome shotgun sequence.
ACCESSION   DASVQZ010000080 DASVQZ010000000
VERSION     DASVQZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115415"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="recJ"
                     /locus_tag="VF547_02225"
     CDS             complement(<1..1383)
                     /gene="recJ"
                     /locus_tag="VF547_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX8261669.1"
                     /translation="MLTTAHVCGVSRSILGQPWRWRGAAADGHDDAFRPDELVDQLLM
                     ARGVERDQLERHRLPTLRGFMPDPSLFRDMDPAAERLADAVEAGEAVTVFGDYDVDGA
                     TSAALLVRLLRALGSEPRAYIPDRLMEGYGPSGEALVRLAGEGARLIVTVDCGAQAFE
                     ALAMARAAGVDVIVVDHHKCASELPVALALVNPNRLDEAPDAAAHGHLAAVGMCFLLG
                     AATVRVLRARGFFARREEPRLLDLLDLVALGTVADVAQLRGLNRAFVTQGLKVMAQRR
                     NTGLAALADAARLSRAPTCADLGWALGPRINAGGRVGKSDLGVRLLTTEDRAEAEAIA
                     AELDRLNEERRAIETMVCEAAEALCGGQDNRSVVLVAKPGWHPGVIGIVAGRLKERFG
                     RPAIVVALDETGIGKGSGRSIGGVDLGAAVLAAKDSGLLVAGGGHAMAAGLTVERGRI
                     EALADFLDDRL"
     gene            1467..2084
                     /locus_tag="VF547_02230"
     CDS             1467..2084
                     /locus_tag="VF547_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8261670.1"
                     /translation="MTDDDRAEEAALAASIRLSPIATVLSNPRLPDNPIVAANAAFCA
                     LTGYEEREIVGRNCRFLAGPDTERWLTERIRAALRGLAPSLTEILNYKKDGTAFRNAV
                     LIAPLFGASGEVAWFLGSQVEVGEGSAAPLALRQERAVRLAAALSPRQLDVLREMASG
                     QRNKQIAWHLGLSEKTVKMHRALLLEKLGVRTTADAIRLAVEAGL"
     gene            2302..2850
                     /locus_tag="VF547_02235"
     CDS             2302..2850
                     /locus_tag="VF547_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX8261671.1"
                     /translation="MSKPAIAAALLALAAPAAFAASKAPMVGGAAMYPSRTIVENAAR
                     SKAHTTLVAAVKAAGLVDTLSGAGPFTVFAPTNAAFARLPAGTVDTLLRPENKPMLQS
                     VLTYHVVPGRVSAAQLIGKIKAGRGVARLATVQGGTLTARLAGGKVVLTDEKGGRATV
                     TQADVYQSNGVIHVTDAVSLPR"
     gene            complement(2888..3715)
                     /locus_tag="VF547_02240"
     CDS             complement(2888..3715)
                     /locus_tag="VF547_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8261672.1"
                     /translation="MSLAVFLAVLFAALLHAGWNALVKTGLDRFSSILFLGLVQGAIA
                     LLLLPFFGLPARAAWPWVLAGSALHSGYKLALIRAYGHGDLSQVYPLSRGAAPLIVAV
                     AGALFLGETMDAARTAAVVAIAAGIMLMAGRGGLTRAGIGWALVTAGFTAAYTLADGV
                     GARVAGSASAFILTMIALDGLIMLAFGLAARGAAGIAAMLPAWRPGTAAGAMSLGAYW
                     IAIWAFTQAPLATVAALRETSVLFAMLIAAVLLKEKLSPRRWTAGALILAGVVLMRV"
     gene            3810..>4899
                     /locus_tag="VF547_02245"
     CDS             3810..>4899
                     /locus_tag="VF547_02245"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8261673.1"
                     /translation="MARDDAGSDASARLDETPGFRAFVSYSHADTVAARRLQRRLETY
                     RVPRRLADRVAPMGAAQGRVGPVFRDREDLPASADLSEAVKHALARSQALIVLCSPDA
                     ARSLWVAREIQLFRSIHPDRPVLAALIRGSPEEAFPAELRRDGAEPLAADFRRAGDGR
                     KLAFLKVVAGILGIPLDELIQRDGQRRQRRVAAVMAAAVAAIVVLGATTIVAVEARRE
                     AELQRAEAQRQRAEAEGLVEYMLSDVRPSLKSVGRLDVQHKLNERAMRYYAGQGALDA
                     LSDDSLERRARVIGATGEDYENEGYFDKALLHYRTVHASTRALLAKAPGNPTRVLAHA
                     RSENRLALLSITRAQQAETRLQQDGRRPE"
BASE COUNT          720 a         1854 c         1670 g          655 t
ORIGIN      
        1 gagccgatcg tcgaggaagt cggccagcgc ctcgatccgg ccgcgctcga cggtgaggcc
       61 cgccgccatc gcatgcccgc cgcccgcgac gagcagtccg ctgtccttcg ccgccaacac
      121 cgcggcgccg agatcgactc cgccgatcga gcggcccgag cccttgccga tccccgtctc
      181 gtcgagggcg accacgatcg ccggccggcc gaaccgctcc ttgagccggc cggcgacgat
      241 tccgatcacg ccgggatgcc agccgggctt cgcgaccagc accaccgagc ggttgtcctg
      301 gccgccgcac agggcctccg ccgcctcgca gaccatcgtc tcgatcgccc ggcgctcctc
      361 gttcagccgg tcgagctcgg cggcgatcgc ctcggcttcg gcccgatcct cggtggtcag
      421 cagccggacg ccgaggtccg acttgccgac ccggccgccg gcgttgatcc tgggcccgag
      481 cgcccagccg agatcggcgc aggtcggcgc ccggctcagc cgagccgcgt cggcgagggc
      541 ggcgaggccg gtgttgcggc gctgcgccat caccttgagg ccctgggtga cgaaggcccg
      601 gttgaggcct ctcagctgcg ccacgtcggc gaccgtgccg agcgcgacca ggtcgagcag
      661 gtcgagcagc cgcggctcct cgcggcgggc gaagaagccc cgcgcgcgaa gcacccggac
      721 ggtcgccgcg ccgagcagga agcacatgcc gacggcggcg agatggccgt gcgccgcggc
      781 gtcgggggcc tcgtcgagcc ggttgggatt gaccagggcc agggcgacgg gaagctcgct
      841 cgcgcatttg tgatggtcga cgacgatgac gtcgaccccg gcggcccgcg ccatcgccag
      901 cgcctcgaac gcctgggcgc cgcaatcgac ggtgacgatc agtcgcgcgc cctcgcccgc
      961 cagccgcacc agcgcctcgc cggacggtcc gtaaccttcc atcagccggt cgggaatgta
     1021 ggcccgaggc tccgatccga gcgcccggag cagccgcacc agcagggcgg cggacgtcgc
     1081 cccatcgacg tcgtagtcgc cgaacaccgt caccgcctcg cccgcctcga ccgcgtcggc
     1141 gagccgctcg gcggccgggt ccatgtcccg gaacagcgac gggtcgggca tgaagccgcg
     1201 cagggtcggc agccggtgcc gctccagctg atcgcgctcc acgccgcggg ccatcagcaa
     1261 ctggtcgacc agctcgtccg gccggaaggc gtcgtcatgg ccgtccgcag ccgcgccgcg
     1321 ccagcgccag ggctggccga ggatcgagcg cgagactccg cagacatggg cggtggtgag
     1381 catcgcggtg agcctagcgc attgcgagtc cgccgtgaat cgtccttctc gcttctatcc
     1441 acatctgttt tcgctaatct cgcgagatga cggacgacga tcgggcggag gaagcggcgc
     1501 tggcggcatc gatccgcctc agcccgatcg cgaccgtgtt gagcaacccg cgcctccccg
     1561 acaatccgat cgtcgccgcc aatgcggcct tctgcgctct gacgggctac gaggagcggg
     1621 agatcgtcgg ccggaactgc cgcttcctgg ccggtcccga caccgagcgg tggctgaccg
     1681 agcgcatccg ggcggcgctg cgcggattgg cgccgtcgct caccgagatc ctcaattaca
     1741 agaaggacgg caccgccttc cgcaacgccg tcctcatcgc gcccctgttc ggcgcctcgg
     1801 gcgaggtcgc ctggttcctc ggctcgcagg tggaggtcgg cgaaggctcg gccgcgccgc
     1861 tcgctctgcg ccaggagcgc gccgtgcgcc tcgccgcggc cctgtcgccg cgtcagctgg
     1921 acgtgctccg cgagatggcg tcgggccagc gcaacaagca gatcgcctgg catctcggcc
     1981 tgtcggagaa gacggtgaag atgcaccgcg cgctcttgct cgagaagctc ggcgtcagga
     2041 cgaccgccga cgccatccgc ctggcggtcg aggcgggcct ctaattcctc cccggcattt
     2101 gccggggagg gggaccatgc gaagcctggt ggaggggtct tttccgcgga cttcgaagga
     2161 cccctccacc gcgctccgcg cggtccccct ccccgcggaa tcgcggggag gatttcttac
     2221 cctaccgcct aaagtccgta tcgtccggcc gccgcgccgg gcctattcgc cgcggcctcc
     2281 ccccttcgca acgaggtccc catgtccaag cccgctatcg ccgccgccct cctcgccctc
     2341 gccgctcccg ccgccttcgc cgcctccaag gcgccgatgg tcggcggcgc ggccatgtat
     2401 ccgtcgcgca cgatcgtcga gaacgccgcg cgctccaagg cccacaccac gctggtcgcc
     2461 gccgtcaagg ccgccgggct ggtcgacacg ctctccggcg ccgggccgtt caccgtcttc
     2521 gccccgacca acgccgcctt cgcccgcctg cccgccggca ccgtcgacac cctgctgcgg
     2581 cccgagaaca agccgatgct ccagtccgtc ctcacctacc atgtcgtccc gggccgggtc
     2641 agcgccgccc agctcatcgg caagatcaag gcgggacggg gcgtggccag gctcgccacc
     2701 gtccagggcg gcaccctcac cgcccggctc gccggcggca aggtcgtcct gaccgacgag
     2761 aaaggcggcc gcgccaccgt cacccaggcc gacgtctacc agtccaacgg cgtcatccac
     2821 gtcaccgacg ccgtctccct gccccgctga cgagattcct ccccgagccc gcctcgggga
     2881 ggaactccta cacccgcatc agcaccacgc cggccaggat cagcgccccc gccgtccagc
     2941 gccgtgggga gagcttctcc ttgagcagca ccgccgcgat cagcatcgcg aacagcacgc
     3001 tggtctcgcg cagggccgcg accgtcgcca gcggcgcctg ggtgaaggcc cagatcgcga
     3061 tccaataggc gccgagcgac atcgccccgg cggccgttcc cggacgccag gccggaagca
     3121 tggcggcgat gccggcggct ccgcgggcgg cgagcccgaa ggcgagcatg atcaggccat
     3181 cgagggcgat catcgtcagg atgaaggccg acgccgagcc cgcgaccctc gccccgaccc
     3241 cgtccgccag ggtgtaggcg gcggtgaagc cggcggtgac cagcgcccag ccgatcccgg
     3301 ctcgggtgag cccgccccgg ccggccatca gcatgatccc agccgcgatc gcgaccactg
     3361 ccgccgtcct cgccgcgtcc atcgtctcgc cgaggaagag cgcgccggcg acggccacga
     3421 tcaagggggc ggcgcctctg gacagcggat agacctggct gagatcgcca tggccgtaag
     3481 ctcggatgag ggcgagcttg taaccgctgt gaagcgccga tcccgccagc acccagggcc
     3541 aggccgcccg ggcgggcaaa ccgaagaagg gcagcagcag gagcgcgatg gcgccctgta
     3601 ccaggccgag gaacaggatc gacgagaacc ggtcgaggcc ggtcttgacg agggcgttcc
     3661 accccgcgtg gaggagcgcg gcgaagagga cggcgaggaa gacggcgagg ctcatccgtt
     3721 ttggcggtta tacgaggcta tatgctggtt tcacatgtgt ttttcggata ggcattgatc
     3781 ttctgtggct tgtgcggggg gcgcggctgt tggccaggga cgatgcggga agcgacgctt
     3841 cggcgcggct cgacgagacc ccgggcttcc gcgctttcgt cagctacagc cacgccgaca
     3901 ccgtcgccgc ccggcgcttg cagagacggc tcgagaccta tcgagttccc cggcgcctcg
     3961 ccgaccgggt ggcgccgatg ggcgcggcgc agggccgcgt cgggccggtc ttccgggacc
     4021 gggaggacct gcccgccagc gcggacctca gcgaggccgt caagcacgcg ctcgcccgct
     4081 cccaggcgct gatcgtcctc tgctcgcccg acgccgcgcg ctcgctttgg gtcgcgcgcg
     4141 agatccagct gttccgctcg atccaccccg atcgtccggt gctggcggcg ctgatccgcg
     4201 ggagtcccga ggaggccttc cccgccgagc ttcgaaggga tggcgccgag ccgctcgccg
     4261 ccgacttccg ccgggccggg gacggccgca agctcgcctt cctcaaggtc gtggccggaa
     4321 tcctcggaat cccgcttgac gagcttatcc agcgcgacgg ccagcgccgc caaaggcgcg
     4381 tggcggcggt catggccgcc gccgtcgcgg ccatcgtggt gctcggagcc acgacgatcg
     4441 tcgccgtcga agcccgccgc gaagccgagc tccagcgcgc cgaagcccaa cgccagcgcg
     4501 ccgaggccga agggctggtc gaatatatgc tgtccgacgt gaggcccagc ctcaagtcgg
     4561 tcggccggct cgacgtccag cacaagctca acgagcgggc gatgcgttat tacgccggcc
     4621 agggcgcgct cgacgctctg tccgacgaca gcctcgagcg ccgcgcgcgg gtgatcggtg
     4681 cgaccggcga ggattacgag aatgagggct atttcgacaa ggccctgctc cactaccgca
     4741 ccgtgcacgc cagcacccgc gcgctgctcg ccaaggctcc cggcaacccg acccgggtgc
     4801 tcgcgcacgc gcgcagcgag aatcggctcg ctctgctttc gatcacccga gcccagcagg
     4861 ccgaaacccg gttgcagcag gatggacggc ggcccgagc
//
LOCUS       DASVQZ010000081         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_786733, whole genome shotgun sequence.
ACCESSION   DASVQZ010000081 DASVQZ010000000
VERSION     DASVQZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786733"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF547_02250"
     CDS             <1..533
                     /locus_tag="VF547_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261674.1"
                     /translation="ERLFVYVGYSHAAEAAMASPGGPVEWMATRLKRLTGIDPLTIDQ
                     TVIDETSARRRAYRDLVAPRLGGRPGILFSSGRPLVEGEYAGVVDLQVVHPPLRLVHG
                     RPEWMRRIGRRPVDIPRRLLPERGKRLVQAFAAGEPDDATPLDQIIVEAGQRAPPLLL
                     PRGRFRWAVQDPPPGR"
     gene            600..1562
                     /locus_tag="VF547_02255"
     CDS             600..1562
                     /locus_tag="VF547_02255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8261675.1"
                     /translation="MRHAVFAAAALALGLSACVQVRQDEDARQSTRAAGWRPVPVPGT
                     REPSEVVAATTAADWRAVDPENLILMELADGQRVAIELAPAFAPVHAANIRAFARAGW
                     WNNAAIYRVQDNYVSQWGNGDAENPLPAGVIRTPPAEYDRALAGLNVRPLGYPDSYAP
                     MAGHVDGWPVAWDPESGRAWLTHCYGSVGAGRDEAPDTGTAGELYAAIGHAPRHLDLN
                     IATVGRVIEGMAALSARPRGTGNLGFYDPAKGEQPIRIARVRVAADVPASERPSFEVM
                     RTDTAAFSAYVTGRANRGGSFFNRPAGGTDLCNVNVPVRRRPAG"
     gene            complement(1817..2089)
                     /locus_tag="VF547_02260"
     CDS             complement(1817..2089)
                     /locus_tag="VF547_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261676.1"
                     /translation="MAISVLEARGGSLVPPASQRGYHIVYRDHETNYCPGCGRTHWYI
                     GRLSAECAFCATALPLENASLVAGGQIVRHVRRSGFGPGEQHAFAA"
BASE COUNT          318 a          768 c          791 g          313 t
ORIGIN      
        1 gcgagcggct cttcgtctat gtggggtaca gccacgccgc cgaggcggcg atggcgagcc
       61 ccggcggacc tgtcgaatgg atggccacgc ggctcaagcg cctcaccggc atcgatccgc
      121 tcaccatcga tcaaaccgta atcgacgaga ccagtgcccg ccgccgcgcc tatcgcgacc
      181 tcgtcgcgcc gcgcctgggc ggacgtcccg gcatcctctt ctcgagcggc cggccgctcg
      241 tcgaaggcga atatgcaggc gtggtcgatc tccaggtcgt ccacccgcct ctgcggctgg
      301 tccatggccg gccggagtgg atgcgccgga tcgggcggcg cccggtcgat atcccgcgtc
      361 ggctgctgcc ggagcgcggg aagcggctcg tccaggcctt cgccgccggc gagcccgacg
      421 atgcaacgcc gctcgaccag atcatcgtcg aggcggggca gcgggcacct ccgctccttc
      481 ttccgcgcgg gcgcttccgt tgggcggtgc aggacccgcc gcccgggcgc tgagcaggtc
      541 gggcttgatc gcgccgcagt ttccggcacg gtggcgctgc atcaacaggg gtcctcgcca
      601 tgcgccacgc cgtcttcgcc gcagccgctc tcgcgctcgg cctttccgcc tgcgtgcagg
      661 tgcgccagga cgaggacgcc cggcagtcga cgcgcgccgc cggatggcgc cctgtcccgg
      721 tacccgggac acgcgaaccg tcggaagtcg tcgcggccac gaccgctgcg gactggcgcg
      781 ccgtcgatcc ggagaatttg atcctcatgg agcttgccga cgggcagcgc gtcgcgatcg
      841 agctcgcccc ggcctttgcg ccggtccacg ccgcgaacat ccgcgccttc gcgcgggccg
      901 gctggtggaa caacgccgcc atttaccggg tgcaggacaa ttacgtctcg caatggggca
      961 atggcgatgc cgagaacccg ctgcccgccg gcgtgatccg gacgccgccg gccgaatacg
     1021 accgggcgct cgccggcttg aacgttcggc cgctcggcta tcccgacagc tatgcgccga
     1081 tggccggcca tgtcgacggc tggccagtcg cctgggaccc ggagagcggc cgcgcctggc
     1141 tcacccattg ctacggcagc gtcggcgccg gacgcgacga ggcgcccgac acgggcacgg
     1201 ccggcgagct ctacgcggct atcggccacg cgccgcgtca cctcgacctc aacatcgcga
     1261 cggtcggtcg ggtgatcgag ggcatggcgg ccctgtctgc gcggccgcgt ggaaccggca
     1321 atctcggctt ctacgatccg gcaaagggcg agcagccgat ccgcatcgcg cgggtgcggg
     1381 tcgccgccga tgttccggca agcgagcggc cctccttcga agtgatgcgc accgacacgg
     1441 cggccttctc ggcctacgtc acggggcgcg caaaccgcgg cggcagcttc ttcaaccgcc
     1501 ccgcaggcgg caccgacctg tgcaacgtca acgtgccggt gcggcggcgg cccgcgggct
     1561 gaagaggttc ggggcctgaa acagttgcgg gtcccgccgg agagggaggc tccgggggac
     1621 ccgcctgttg aggcgccggc ggggggacga accgcctttc gcgccccgga tcggcttcgt
     1681 cgcggttgcg gccgcgatga agccgcgggt cggggcagag gggaggtttg cccggaccct
     1741 gaggcaagag cgggaggggg aagccgctcg cgcctcggta gctcagctta ggcggccgta
     1801 gccgcgcagc cgcaggtcag gcggcgaaag cgtgctgttc gcccgggccg aagccggagc
     1861 ggcggacgtg gcggacgatc tggccgccgg cgacgaggga ggcgttctcc agcggcagcg
     1921 cggtcgcgca gaaggcgcat tccgcggaca gccggccgat gtaccaatgg gtgcggccgc
     1981 agccggggca gtaattggtc tcgtgatcgc gatagacgat gtggtaccca cgctggctgg
     2041 ctggcgggac aaggcttccg ccgcgggctt caagaacgct aatcgccatt acattggctc
     2101 ccttctcaca aactcgaact tgaccggccc aacgcgccgg gagtcggaga agtttcatgg
     2161 atttagcatg aacggggacg agcgccgcca
//
LOCUS       DASVQZ010000082         4753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_497396, whole genome shotgun sequence.
ACCESSION   DASVQZ010000082 DASVQZ010000000
VERSION     DASVQZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497396"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="VF547_02265"
     CDS             complement(<1..2041)
                     /locus_tag="VF547_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX8261677.1"
                     /translation="MADDERQATRLQVATMRPEDSGRGLARLPRRLMAALALAEGDVI
                     EVTGKRSTPARAVGPYAEDEGLDIVRLDGLQRANAEIGSGDFVTIRKVESKPAQRVVF
                     APAQKNLRLHGSAQALKRSFGMKPLTAGDVVATTGQQQIQRGDIPPELRQMLNAPAYA
                     LQEIRLTVVSTAPKGVVHIDANTEVELRPEYAEATDQRRADVTYDDLGGIGTTIDQLR
                     EMVELPLRYPEIFERLGVDPPKGVLLYGPPGTGKTRLARAVANESDASFFHIAGPEIM
                     GSAYGESERRLREVFEEAAKSAPAIIFIDEIDSIAPKRGQVQGETEKRLVAQLLTLLD
                     GLEPRQNLVVIAATNRPEALDEALRRPGRFDREIIIGVPDEGGRREILAIHTRGMPLD
                     GGVNIKELARRTFGFVGADLAALVREAAIEAVRRIMPQINLEDGTIPPEVLETLSVKG
                     SDFEEALKRVQPSAMREVMVQAPTTRWEDVGGVDEARERLREGVELPLKSPEAFKRMG
                     IRPAKGFLLYGPPGTGKTLLAKATAREAEANFIAVKSSDLLSKWYGESEQQIARLFAR
                     ARQVAPTVIFIDELDSLVPARGGGLGEPQVTERVVNTILSEMDGLEDLGSVVVIGATN
                     RPNLIDPALLRPGRFDELIYVSVPNEAGRRHILGIHTQSMPLAPDVDLDALARRTE"
     gene            2558..3451
                     /locus_tag="VF547_02270"
     CDS             2558..3451
                     /locus_tag="VF547_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8261678.1"
                     /translation="MRRLPPLTALEAFIQVARLGSVKAAAEALALSSPALSRRIQALE
                     RFVGHPLFERRHQAVHLNPDGERLMAEIGPSIDALALAMERATGNVEMMRLRLAVPSL
                     FASQRLMPSLPSLRALHPDLHIDLDTGPNRLARLDEGLDAAIAITTEIDPSLYSRRLD
                     SNCVIAIGSRALQQTIREPAQLAEATIFLHRDMPETFDYWREAVGLPGLQPAAIDHFD
                     SGQLILDAAAQGLGVAFMLESHLYGSGDERLVRLFDHRVETPYSYWFACRRSALKRRP
                     VRIFHDWLFEVLLPGEMREAA"
     gene            complement(3542..4300)
                     /locus_tag="VF547_02275"
     CDS             complement(3542..4300)
                     /locus_tag="VF547_02275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8261679.1"
                     /translation="MARGVTLITGASAGLGAEFARQCRKRGETVALVARRKDRLEALR
                     ASLGEAVFVHAADLTRPDQVEALLPWLESEGLEIETLINNAGFGATGTFADTPRERLL
                     EMIDLNVRSLTDLCRLVLPSMIERRRGGILNVASTAAFQAGPYAAVYYATKAYVLSLT
                     EALHHEAKGSGVRVSALCPGPTATEFFEVAGAPDSKLAKVAVRPEGVVAAGLRGLDRN
                     RPIVIPGIANNIGAFGTRLMPRAALRRIVASLKA"
     gene            complement(4303..>4753)
                     /locus_tag="VF547_02280"
     CDS             complement(4303..>4753)
                     /locus_tag="VF547_02280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8261680.1"
                     /translation="LNTLVLNLDSGGDVVIKLRPDLAPGHVARIRELVEEGFYDGVVF
                     HRVIPGFMAQGGDPTGTGMGGSDKPDLKAEFSAAPHVRGVASMARSSNPNSANSQFFI
                     CFDDARFLDNQYTVWGEVTEGMEHVDALPKGEPPREPGKIVKASLRP"
BASE COUNT          681 a         1594 c         1692 g          786 t
ORIGIN      
        1 gctcggtgcg gcgggcgagc gcgtcgaggt cgacgtcggg ggcgagcggc atgctctggg
       61 tgtggatgcc gaggatgtgg cgccggccgg cctcgttcgg cacggagacg tagatcagct
      121 cgtcgaagcg gccgggacgg agcaaggcgg ggtcgatcag gttcgggcgg ttggtggcgc
      181 cgatcaccac caccgagccc aggtcctcga ggccgtccat ctcggagagg atggtgttga
      241 ccacgcgctc cgtcacctgc ggctcgccga ggccgccgcc gcgggcgggg acgaggctgt
      301 ccagctcgtc gatgaagatc acggtcgggg cgacctggcg ggcgcgggcg aacaggcggg
      361 cgatctgctg ctcgctctcg ccataccatt tggacagcag atcggaggac ttgacggcga
      421 tgaaattggc ctccgcctcg cgcgcggtcg ccttggcgag cagggtcttg ccggtgcccg
      481 gcgggccgta gaggaggaag cccttggccg gccggatgcc catgcgcttg aaggcctcgg
      541 ggctcttcaa aggcagctcg acgccctcgc gcagccgctc gcgcgcctcg tcgaccccgc
      601 cgacgtcctc ccagcgggtg gtcggcgcct ggaccatcac ctcgcgcatc gccgagggct
      661 ggacccgttt gagcgcctcc tcgaagtcgc tgcccttgac cgagagggtt tcgagcacct
      721 cgggggggat ggtgccgtcc tcgaggttga tctgcggcat gatccggcgg accgcctcga
      781 tcgccgcctc gcggaccaga gcggcgaggt cggcgccgac gaagccgaag gtgcggcggg
      841 ccagctcctt gatgttgacc cccccatcga gcggcatgcc tcgggtgtgg atcgccagga
      901 tctcccgccg tccgccctcg tcggggactc cgatgattat ctcgcggtcg aagcggccgg
      961 gccgcctcag cgcctcgtcg agcgcctcgg gccggttggt ggcggcgatg acgacgaggt
     1021 tctgcctggg ctcgaggccg tcgagcaggg tcagcagctg ggcgacgagg cgcttctcgg
     1081 tctcgccctg gacctggcct cgcttgggcg cgatcgaatc gatctcgtcg atgaagatga
     1141 tcgccggcgc cgacttcgcc gcctcctcga acacctcccg caggcgccgc tcgctctcgc
     1201 cataggccga gcccatgatc tcggggccgg cgatgtggaa gaagctggcg tcgctctcgt
     1261 tggcgacggc tcgggcgagg cgggtcttgc cggtgcccgg cgggccgtag agcagcacgc
     1321 ccttgggcgg atcgaccccg agccgctcga atatctccgg atagcgaagc ggcagctcga
     1381 ccatctcgcg gagctggtcg atcgtcgtcc cgattccgcc gagatcgtcg taggtgacgt
     1441 cggcgcggcg ctgatccgtg gcctcggcat attcggggcg gagctcgacc tcggtattgg
     1501 cgtcgatatg gacgacgccc ttgggcgcgg tcgagacgac ggtgagccgg atctcctgca
     1561 gcgcgtaggc cggggcgttg agcatctggc gaagctcggg cgggatgtcg ccgcgctgga
     1621 tctgctgctg gcccgtcgtg gcgacgacgt cgccggcggt cagcggcttc atgccgaagc
     1681 tgcgcttcag cgcctgggcc gagccgtgca ggcgcaggtt cttctgggcc ggagcgaaga
     1741 cgacgcgctg cgcgggcttc gattcgacct tgcggatggt gacgaagtcg cccgagccga
     1801 tctccgcatt ggcgcgctgg agcccgtcga ggcggacgat gtcgagcccc tcgtcctcgg
     1861 cataggggcc gaccgctcgg gccggggtcg agcgcttgcc ggtgacctcg atgacgtcgc
     1921 cctcggcgag ggccagggcg gccattagcc gccgcggcag ccgtgccagg ccgcgccccg
     1981 aatcctccgg ccgcatcgtc gcgacctgga gccgggtcgc ctgtctctcg tcgtccgcca
     2041 tcgggccggc cttccttcgt ttccgtcgcc gtgaaatagg aagggggaac ggcgttgcca
     2101 cctcgccggg tccgaagagt cgaaaaaagg gagccgcgtc ggttcgcgcg attggcctca
     2161 ggacgcggtg cgagcccatt ttctagacaa ggcgcgaagg cgcgaaggca cgaagcgtag
     2221 gcttgggctg tcggccgaag gccgcataat cctcaccctc agactgtgct tcgtgccttc
     2281 gtgccttcgg gcctttcctc caaaaatgag ggcgagctac ggtcggaggc aaagtcgctg
     2341 gaagcgcgaa aaaaaaaggg ccggtcccgg aaggaccgac cctgaaagtt tttaggagag
     2401 gatgcctgaa aggcagggtc tttctccccc tccgacgttt attgtgcaag tgcgaacaag
     2461 tatgctacag atgcatttgt tgcacttgct ttccgaggga tgacgagcgt gccggcccga
     2521 ttgcaagctg ggtgcagcgc acaataggag gccgaagatg cgccgattgc cgccgctcac
     2581 cgccctcgaa gccttcatcc aggtcgcccg cctcggctcg gtcaaggcgg ctgccgaggc
     2641 gctcgccttg tcgtcgcccg ctttgtcgcg ccggatccag gcgctggagc ggttcgtcgg
     2701 ccacccgctg ttcgagcggc gccaccaggc cgtccacctc aatcccgacg gcgagcggct
     2761 gatggcggag atcggcccgt cgatcgacgc gctcgccctg gccatggagc gcgcgaccgg
     2821 caatgtcgag atgatgcgcc tcaggctcgc cgtgccgtcg ctgttcgcct cgcagcggct
     2881 gatgccaagc ctgccttcgc tgcgggcgct gcatcccgat ctccatatcg atctcgatac
     2941 cgggcccaac cggctcgccc ggctcgacga gggtctcgac gcggcgatcg ccatcaccac
     3001 cgagatcgac ccgtcgctct actcgcgccg gctcgacagc aattgcgtca tcgcgatcgg
     3061 atcgcgcgcg cttcagcaga cgatccgcga gccggcccag ctcgccgaag ccaccatctt
     3121 cctccaccgc gacatgcccg agacgttcga ctattggcgc gaggcggtcg gcctgcccgg
     3181 cctccagccg gccgcgatcg accatttcga ttccggccag ctgatcctcg acgccgccgc
     3241 ccagggcctc ggagtcgcct tcatgctcga gagccatctc tacggatcgg gcgacgaacg
     3301 cctggtgcgg ctgttcgacc accgggtcga gaccccctac agctactggt tcgcctgccg
     3361 ccgctcggcc ctgaagcgcc gccccgtcag gatcttccac gactggctgt tcgaggtgct
     3421 tctgcccggc gagatgcggg aggcggcctg accgccgtca ttgcgagcga agcgaagcaa
     3481 tccagtccgg cgcctcagcc gcgctggatt gcttcgtcgc ttcgctcctc gcaatgacgg
     3541 cctaagcctt cagcgaggcg acgatccgcc gcagcgcggc gcgcggcatc agccgggtgc
     3601 cgaaggcgcc gatattgttg gcgatgccgg ggatgacgat cggccggttg cggtcgaggc
     3661 ccctgaggcc cgccgcgacg acgccttcgg gccggacggc gaccttggcg agcttcgagt
     3721 ccggcgctcc ggccacctcg aagaactccg tcgccgtggg tccggggcag agcgcgctca
     3781 ccctcactcc gctgcctttc gcctcgtggt ggagcgcttc ggtcagcgac agcacatagg
     3841 ccttggtggc gtaatagacg gcggcatagg ggccggcctg gaaggcggcg gtcgacgcca
     3901 cgttgaggat gccgccgcgc cggcgctcga tcatgctggg aaggacgagg cggcagaggt
     3961 cggtcagcga gcggacgttg aggtcgatca tctccaggag gcgctcgcgc ggcgtgtccg
     4021 cgaaggtgcc ggtggctccg aagccggcat tgttgatcag cgtctcgatc tcgaggcctt
     4081 cggattccag ccaggggagc agcgcttcga cctggtcggg gcgggtgagg tcggcggcgt
     4141 ggacgaagac ggcctcgccg aggctcgcgc gcagcgcctc gagccggtcc ttgcgcctcg
     4201 ccaccagggc gacggtctcg ccgcgcttgc ggcactggcg ggcgaactcg gcgccgaggc
     4261 ccgccgaggc gccggtgatg agcgtgaccc cccgcgccac cgtcagggcc tcagcgacgc
     4321 tttgacgatc ttgcccggct cgcgcggcgg ctcgcccttg ggaagggcgt cgacatgctc
     4381 catgccctcg gtgacctcgc cccagacggt gtactggttg tcgaggaagc gggcgtcgtc
     4441 gaagcagatg aagaactggc tgttggcgct gttcggattg gacgagcgcg ccatcgaggc
     4501 gaccccgcgg acgtggggtg ccgcgctgaa ctcggccttg aggtcgggct tgtcggagcc
     4561 gcccatgccg gtgccggtcg gatcgccgcc ctgggccatg aagcccggga tcacacgatg
     4621 gaagacgact ccgtcgtaga agccctcctc gaccagctcg cggatcctgg cgacgtggcc
     4681 cggcgcgagg tcgggcctga gcttgatgac gacgtcgccg ccgctgtcga ggttgaggac
     4741 cagggtgttg agc
//
LOCUS       DASVQZ010000083         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_307637, whole genome shotgun sequence.
ACCESSION   DASVQZ010000083 DASVQZ010000000
VERSION     DASVQZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307637"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..2418)
                     /locus_tag="VF547_02285"
     CDS             complement(112..2418)
                     /locus_tag="VF547_02285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX8261681.1"
                     /translation="MSSRLARNLLLTTVCAAGLLLAGCGGGGGGTGGGPAPIAAPPPA
                     PAPPPPPPPPPPPPPPPPTGTNFNDAEYARSNGTSAHASIAAYNSGATGRGVKIAVVD
                     TGLNPNLPEFAGKVDPASRDIAANRGITDTEGHGTAVSAVAAAARDGFGVMGVAFDST
                     ILSFNTSDPTDCDPDDGCTHASVDIAEAIDLARINGARVINISLGGPDPSAAVNAAIA
                     RAAAAGVLIVMSAGNVGDEPGGENPESFSLSAAAAGNVIIAGAMDASRNSATFSNKAG
                     TGASRYLTAIGVRVRAPDHTGTQFLWSGTSFSAPVITGAAALLASAFPNLTGQQIMAI
                     LLNSADDAGAAGTDPIFGRGILNVERAFLPQGTLSLPGGQEVDPSASSGSGSTTMGDA
                     KAGVEGMIVLDGYSRAYAMTLASALQRAEQERPLGQALQPGLTTATSGARGVAVSITV
                     DRKLTGQPQVGLAQLGLTYEDARTARVLSGIAVGKLGPRMAAAMGLSESAKTLERRLT
                     GHHQDAFLVARDPMSRTGFHGDSAAALGLRRQVGRIGVTLTAERGEVFQPGFDRSIAR
                     PRYTVASIGADRRFGRATFSLGASRLDEESTVLGARFSPTIGVGGGASWFADANASLD
                     LGRGWGARAAYRRGWTATAGTGGLAQRGRLETDAWSLDLAKTGAFRPGDRFALRLMQP
                     LRVRSGGFDLSVPVSYSYDTLSAGYENRFFNLAPTGREIDFEAAYGFGLLDGAGHLSA
                     NAFARRHPGNVAAMDTDLGAAIRFTLGL"
     gene            2532..3194
                     /locus_tag="VF547_02290"
     CDS             2532..3194
                     /locus_tag="VF547_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX8261682.1"
                     /translation="MPAPLASIDCWIFDLDNSLYPAAANLFELIDVRMGEYIARRLGC
                     EMAEARRIQKGFFRDHGTTLAGLMKEHGVEPRDFLDYVHDIDLARITADPRVVAALDR
                     LPGRKFVFTNGDETYARRVLDRLGLANAFDGVHDIHAMDYVPKPDPRAYAAMCDRLAI
                     NPGSALFAEDMARNLVPAKKLGMTTVWVDNGSEQASHDADPAFIDYRITDIGEWLEEI
                     TS"
BASE COUNT          482 a         1101 c         1208 g          476 t
ORIGIN      
        1 gccggggagg gggaccaggc gaagcccggt ggaggggttc ttccggacgc cgaagaaccc
       61 ctccaccggg gctgcgcgcg gtcccctccc cccggaaatc cgggggagga actacaaacc
      121 gagggtgaac cggatcgccg cgccgaggtc cgtgtccatc gccgcgacgt tgccgggatg
      181 gcgacgcgcg aaggcattgg cgctgaggtg accggcgccg tcgagcagac cgaagccgta
      241 agcggcctcg aaatcgatct cgcggcccgt gggcgccaga ttgaagaagc ggttctcgta
      301 gcccgcgctc agcgtatcgt agctgtacga cacggggacg ctgaggtcga agccgccgga
      361 gcgcacgcgc agcggctgca tcaaccgcag ggcgaaacgg tctccgggcc gaaaagcgcc
      421 ggtcttcgcc aggtcgaggg accaggcgtc ggtctcgagc ctgccgcgct gcgccaggcc
      481 gccggtgccg gcggtcgccg tccagccgcg gcggtaggcg gcccgggcgc cccagccgcg
      541 gccgaggtcg agcgaggcgt tggcatcggc gaaccagctg gcccctccgc cgacgccgat
      601 ggtcggcgag aagcgtgcgc cgagcaccgt cgactcctcg tcgagccggc tggcgccgag
      661 gctgaaggta gcccggccga aacggcggtc cgcgccgatc gaggcgacgg tgtagcgggg
      721 ccgggcgatc gaccggtcga agcccggctg gaacacctcg ccgcgctcgg ccgtgagggt
      781 gacgccgatc cggccgacct ggcgccgtaa acccagcgcc gcggcggagt cgccgtgaaa
      841 gccggtccgg ctcatcgggt cgcgggcgac caggaaggcg tcctgatggt gcccggtcag
      901 gcgccgctcc agggtcttgg cgctctccga caggcccatc gccgccgcca tgcgcgggcc
      961 gagcttgccg accgcgatgc ccgacagcac gcgcgccgtg cgcgcgtcct cgtaggtcag
     1021 gcccaattgg gcgagcccga cctgcggctg cccggtgagc ttgcggtcga cggtgatcga
     1081 gaccgcgacg ccgcgggcgc ccgaggtcgc ggtggtgagg cccggctgaa gcgcctggcc
     1141 gagcggcctt tcctgctcgg cgcgctgcaa ggcggaggcc agggtcatcg cataagcgcg
     1201 cgaatagccg tcgaggacga tcatgccttc gacaccggcc ttggcgtcgc ccatcgtcgt
     1261 cgaccctgag ccgctgctcg ccgagggatc gacttcctgg ccgccgggaa ggctgagggt
     1321 gccctggggg aggaaggcgc gctcgacgtt gaggatgccg cggccgaaga tcggatcggt
     1381 gcccgcggcg ccggcgtcgt cggcgctgtt cagcaggatc gccatgatct gctggccggt
     1441 gagattgggg aaggcgctgg cgagcagcgc tgccgcgccg gtgatcacgg gggccgagaa
     1501 ggacgtgccc gaccacagga actgggtgcc ggtgtgatcc ggcgcgcgca cgcgcacgcc
     1561 gatcgcggtg aggtagcgcg acgcgccggt gccggccttg ttcgagaagg tcgcgctgtt
     1621 gcgcgaggcg tccatcgctc cggcgatgat gacgttgccg gcggcggcgg cggagaggga
     1681 gaaggattcg gggttctcgc cgccgggctc gtcgccgaca ttgccggccg acatgacgat
     1741 cagcacgccc gccgccgccg cccgggcgat ggcggcgttc actgcggcgc tgggatcggg
     1801 tccgccgagg gagatgttga tcaccctggc tccgttgatg cgggcgaggt cgatcgcctc
     1861 cgcgatgtcg acgctggcgt gggtgcagcc gtcgtcggga tcgcagtcgg tcggatccga
     1921 ggtgttgaac gacaggatgg tcgaatcgaa ggccacgccc atcacgccga agccgtcgcg
     1981 ggccgcggcg gcgaccgccg agaccgccgt gccgtggcct tccgtgtcgg tgatcccgcg
     2041 attggcggcg atgtcgcggc tggccggatc gaccttgccg gcgaattcgg gaaggttggg
     2101 attgaggccc gtgtcgacga ccgcgatctt caccccccgt ccggtcgcgc cgctgttgta
     2161 cgccgcgatc gacgcgtgcg cgctggtgcc gttggagcgg gcatattcgg cgtcgttgaa
     2221 gttcgtcccc gtcggcggcg gcgggggcgg agggggcggc ggaggtgggg gcgggggcgg
     2281 cgcgggagcc ggcgggggcg cggcgatcgg cgcgggaccg ccgcccgtgc ctccgccgcc
     2341 gccgccgcag cccgcgagga ggagcccggc cgcgcagacg gtcgtcagca gcagattgcg
     2401 tgcaagcctg gagctcatcc ccccaccttt cggctaaaca tctcagaatt ttggactttt
     2461 ccgattcgtt cctacccgtt tgatgctttc attttgctga aggccctccc cgccctatga
     2521 ggcccgccat gctgcccgcc ccgctcgcgt cgatcgactg ctggatattc gacctcgaca
     2581 acagcctcta ccccgccgcc gcgaaccttt tcgagctgat cgacgtccgc atgggggagt
     2641 atatcgcacg acggctggga tgcgaaatgg ccgaggcgcg gcgcatccag aagggcttct
     2701 tccgcgacca cggcaccacc cttgcgggcc tgatgaagga gcatggggtc gagccgcgcg
     2761 atttcctcga ttacgtccac gacatcgatc tggcccgaat caccgccgat ccgcgcgtgg
     2821 tcgcggcgct ggaccggctg ccgggccgca agttcgtctt caccaacggc gacgagacct
     2881 acgcgcgccg cgtgctcgac cggctcggac tcgccaacgc cttcgacgga gtccacgaca
     2941 tccacgccat ggattatgtg cccaagcccg atccgcgcgc ctatgcggcg atgtgcgatc
     3001 gcctcgccat caaccccgga agcgccctgt tcgcggagga catggcgagg aacctggttc
     3061 cggccaagaa gctcgggatg accaccgtct gggtcgacaa cggctccgag caggcgagtc
     3121 acgacgccga tcccgccttt atcgactacc gcattaccga tatcggcgag tggctggagg
     3181 agatcacttc atgaccgagg acctgcgccg gacgatcgag gaagcgtggg agcggcgcga
     3241 ctcgctgtcc ggcggcgagg ccggact
//
LOCUS       DASVQZ010000084         6869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_404203, whole genome shotgun sequence.
ACCESSION   DASVQZ010000084 DASVQZ010000000
VERSION     DASVQZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404203"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1199
                     /locus_tag="VF547_02295"
     CDS             255..1199
                     /locus_tag="VF547_02295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX8261683.1"
                     /translation="MKVIVTRKLPAAVEAGLAARFDVELNPEDAPFDRERLAGAMRRC
                     DVLAATVTDRIDADLIARAGERLRLIANFGVGTDHIDLESASARGVAVTNTPGVLTED
                     TADLVMALILTAMRRLGEGERVLRAGLWRGWGPTDQLGCSLTGKALGIVGMGRIGSAL
                     ARRARAFGMEIHYHNRRPAPDGEARYWPDLDAMLAAVDVVSLNAPYRPDARHLIDRRR
                     LALMRRGAWLINAARGNLVDEEALIEALAAGRIAGAGLDVYPDEPHVDPRLLELPNLV
                     LLPHLGSATLESRTAMGEKVLANIVAFAEGRELPDRVA"
     gene            complement(1214..2620)
                     /locus_tag="VF547_02300"
     CDS             complement(1214..2620)
                     /locus_tag="VF547_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8261684.1"
                     /translation="MFKGLKPILYAGREVWPLVEGGKGVSATNHASSGAWAAAGGIGT
                     VSAVNADSYDPEGKIIPQVYHALTRRERHEELVQYAIDGAVAQVQRAYDIAGGRGAIN
                     INVLWEMGGAQRVLHGVLERTKGLVAGVTCGAGMPYKLSEIAASYGVNYLPIISSDRA
                     FRALWKRAYSKAAEWLAAVVYEDPWLAGGHNGLSNAEDPLKPQDPYPRVRELRETMRE
                     GGIPDSVPIVMAGGVWNLKEWENWIDNPELGQIVFQFGTRPLLTKESPIPEAWKQRLM
                     TLDEGDILLHRFSPTGFYSSAVRNPFLRSLEARSHRQIAFTKEELGDHCFQLDVGVGN
                     RKNYWVTRGDLLHAREWFAQGYTEALKTPDETLIFVTPEEKVQIRKDQADCMGCLSQC
                     AFSSWADNEKNTTGRLADPRSFCIQKTLQDIAHGGPVDQNLMFAGHAAFRFKKDPFYS
                     NGFVPTVKQLVDRILTGD"
     gene            complement(2682..3821)
                     /locus_tag="VF547_02305"
     CDS             complement(2682..3821)
                     /locus_tag="VF547_02305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8261685.1"
                     /translation="MSAVRILHAHSTFGRGGKEARAVRLMNAFGGSAEHAVISASRGE
                     FGAREALAPEVAADFPADAPPLSGLPGLPRLFRLSRYMRRFDLVLTYNWGAFDAVMAR
                     HLFGGPPLVHHEDGFNEDEAGELKTRRNLYRRLGLGAAHRLVVPSARLEAIARKAWGQ
                     PAERVARIANGIDVPRYALPPSQEIPGLEKRPGEVVIGTVAGLRAVKNLPRLVRAFAA
                     MTAPGARLVIVGAGPESERIAAEARRLGVGDRLLMAGFLADPARWIGRFDIFALSSDS
                     EQFPISLVEAMAAGLPAVSTAVGDVTQMVAPDNRPLIVEAEDEAAFAAALDSLAQRPD
                     LRRAIGRANRERAEAEYDEAGMIARYARLYGEAIGRPGAFMPSAR"
     gene            4035..5114
                     /locus_tag="VF547_02310"
     CDS             4035..5114
                     /locus_tag="VF547_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261686.1"
                     /translation="MDWTLGVLSSWPAGLAVGLFSVAYIAAIIWTGIEPRKVAAPFEP
                     PPMPTLTPAEAVIYERTRLAQMRAEEQRALDAEEQARSSREFHRALDAYTRFERRAPP
                     PLPSVGQAHLGTPGAAVFGIDAAESFVAIDLQNFGQTPARDVTYKTLTGWFPQQPREP
                     IAYESEGGVGITDPGHVQHIKLILKGPLRSALIRRSVRGGEGFYVLASVDYTTENGTR
                     KRRKIAYYLDLQGQQLPDRWMLSVCPTGNDELDLGIGDPTAYGDHEPARDVERTERRR
                     ALIKMGRDLAFGYCHGDREETFREYLEGQRGYADIRPHLSEGYRGRLEAVRMVYADPD
                     GAKYPTLVQWFLDELDRLEMEWGLI"
     gene            complement(5280..5612)
                     /locus_tag="VF547_02315"
     CDS             complement(5280..5612)
                     /locus_tag="VF547_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261687.1"
                     /translation="MTMYVYSTAGEPRGFLFETTIFGLDGVPLGRVLGSRVHRFDGSY
                     AGEWFHQMVVDRPTARPRSIPPIAVPASRPPEPVTCWRRHVADYGAHVDAFDRLLAPA
                     EQYQIAAE"
     gene            5772..5996
                     /locus_tag="VF547_02320"
     CDS             5772..5996
                     /locus_tag="VF547_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261688.1"
                     /translation="MKLIVMKKGGEPGEVAINPDRVTHVRTASGPFTDIYFGDHRVAV
                     EGTFAQVVARLTGQAEIPDAPPQKSWFPAR"
     gene            complement(6012..>6869)
                     /locus_tag="VF547_02325"
     CDS             complement(6012..>6869)
                     /locus_tag="VF547_02325"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8261689.1"
                     /translation="RVGEDLTFDGSAETDGKFLVYGGHGTDILKGGAGVDVFFFEGDR
                     WGADDRVDGGGGRDAVVSSGTGLKQVTFGANSLTNIESISVNATLASDPSAKPSYAFV
                     LHNGNVTPGGNLIVNANSLVDPTQTLSVNGSAVVDGSLTMYGGAGNDTLIGGAGADLL
                     YGGGGRDTLTGGGGADTFQLRAAGESLPWQGDSIQDFQGGVDKIDLSRIDADITTPGD
                     QAFTVIGSAQFTGHAGELRIVDMGMNQWLIMADVDGDANADFSTQADLMISVTRTDSS
                     AMLGSDFIL"
BASE COUNT         1102 a         2330 c         2332 g         1105 t
ORIGIN      
        1 aggtcgccgg tttattgcgc ccgggcccgg tgcgcatcat cgccttgccg gcgctgatcg
       61 acgcccaata gggcgtggcc cgatcgtcct gcgcctcggc cggccccacc gtgaggccgg
      121 tcgccaacgc cgccgccagc acgactctcc tcgccatcgc cgcctccttc tccagcctct
      181 taggcaggag cgggccgcag gttcaacggc gatctgcctt gacgctccgc ctcccctttc
      241 cccaagcggg acggatgaag gtgatcgtca cgcgcaagct cccggcggcg gtggaggccg
      301 ggctggccgc gcgcttcgac gtcgagctca accccgaaga cgccccgttc gaccgcgagc
      361 ggctggccgg ggcgatgcgc cgctgcgacg tgctcgcagc cacggtcacc gaccggatcg
      421 acgccgacct catcgcccgc gccggcgagc gcctgaggct gatcgccaat ttcggggtcg
      481 ggacggacca tatcgacctc gaaagcgcga gcgcgagggg cgtggccgtc accaacaccc
      541 ccggagtcct caccgaggac acggccgatc tcgtcatggc gctgatcctg acggcgatgc
      601 ggcgcctcgg cgaaggcgag cgcgtccttc gagccggcct ctggcgaggc tggggcccga
      661 ccgaccagct cggctgcagc ctgaccggga aggcgctcgg aatcgtcggc atgggccgga
      721 tcggctcggc gctggctcgg cgggcgcggg cgttcgggat ggagatccac taccacaatc
      781 gtcgccccgc cccggacggc gaggcccgct actggcccga tctcgacgcc atgctcgcgg
      841 cggtggacgt ggtcagcctc aacgcgccct atcggccgga cgcccgccat ctgatcgacc
      901 ggcggcggct ggcgctgatg cggcgcggcg cctggctgat caacgccgcc cgcggcaatc
      961 tcgtcgacga ggaggcgctg atcgaggcgc tcgccgccgg ccggatcgcc ggcgccgggc
     1021 tcgacgtcta tcccgacgag ccgcacgtcg atccgcgcct gctcgagctt ccgaacctgg
     1081 tgctgctccc ccacctcggc tcggcgaccc tggagagccg caccgcgatg ggcgagaagg
     1141 tcctcgccaa catcgtcgcc ttcgccgaag ggcgggaatt gcccgaccgg gtcgcttgat
     1201 cccgccgctt gactcagtcg cccgtcagga tgcggtcgac cagctgcttc accgtcggga
     1261 cgaagccgtt cgagtagaac ggatccttct tgaagcggaa ggcggcatgg ccggcgaaca
     1321 tcaggttctg gtcgacgggg ccgccgtggg cgatgtcctg gagcgtcttc tggatgcaga
     1381 agctgcgcgg atcggcgagc cggccggtcg tgttcttctc attgtcggcc caggatgaga
     1441 aggcgcactg gctgaggcag cccatgcagt cggcctggtc cttgcggatc tggaccttct
     1501 cctcgggggt cacgaagatc agcgtctcgt cgggagtctt gagcgcctcg gtatagccct
     1561 gagcgaacca ttcgcgcgca tggagcaggt cgccgcgggt cacccaatag ttcttcctgt
     1621 tgccgacgcc gacgtcgagc tggaagcaat ggtcgccgag ctcctccttg gtgaaggcga
     1681 tctggcggtg cgagcgcgcc tcgaggctgc gcaggaaggg attgcggacc gccgagctgt
     1741 agaagccggt gggcgagaaa cggtgaagca ggatgtcgcc ctcgtcgagc gtcatcagcc
     1801 gctgtttcca cgcctccggg atcgggctct ccttggtcag caagggccgg gtgccgaact
     1861 ggaagacgat ctggcccagc tccggattgt cgatccaatt ctcccattcc ttcaggttcc
     1921 agacgccgcc cgccatgacg atcggaacgc tgtcgggaat tccgccctcg cgcatcgtct
     1981 cgcgcagctc cctgacccgc ggataggggt cctgcggctt cagcgggtcc tcggcgttgg
     2041 aaaggccgtt atggcccccg gccagccagg gatcctcgta gacgaccgcg gccagccact
     2101 cggcggcttt cgaataggcg cgcttccaca gcgcccggaa ggcgcggtcg gagctgatga
     2161 tcggcaggta gttgacgccg taggaggcgg cgatctcgga caatttgtag ggcatgcccg
     2221 ccccgcacgt cactccggcg accaggccct tggtccgctc gagcacgccg tggaggaccc
     2281 gctgggcgcc gcccatctcc cacagcacgt tgatgttgat cgccccgcgc ccgccggcga
     2341 tgtcgtaggc gcgctggacc tgcgcgaccg cgccgtcgat cgcatattgg accagctcct
     2401 cgtggcgctc gcggcgggtc agggcatggt agacctgggg tatgatcttg ccttccgggt
     2461 cgtagctgtc ggcattgacc gccgagacgg tgccgattcc tcccgccgcc gcccaggcgc
     2521 cggagctggc gtggttggtc gccgacacgc ccttgccgcc ctcgaccagc ggccacacct
     2581 cgcggccggc atagaggatc ggcttcaatc ccttgaacac atgtttctcc agaaaaaccg
     2641 agagggccta taccgccact ctcgcgcaaa agcgtcaaaa atcatcgcgc cgaaggcatg
     2701 aacgctccag ggcggccgat ggcttcgccg tagagccggg catagcgcgc gatcatcccg
     2761 gcctcgtcat attcggcctc ggcccgctcc cggttggctc ggccgatggc gcggcgcagg
     2821 tcgggacgct gggcgaggct gtcgagcgcg gcggcgaagg cggcctcgtc ctcggcctcg
     2881 acgatcagcg gccggttgtc gggagcgacc atctgggtga cgtcgccgac cgcggtcgac
     2941 accgccggca ggcctgcggc catcgcctcg accagcgaga tcgggaactg ctcgctgtcg
     3001 gaggacagag cgaagatgtc gaagcggccg atccagcgcg ctggatcggc gaggaagccc
     3061 gccatcagca gccggtcgcc gaccccgagc ctgcgcgcct cggcggcgat gcgctcgctt
     3121 tccggcccgg cgccgacgat gacgaggcgg gcgcccgggg ccgtcatggc ggcgaaggcg
     3181 cgcaccaggc gcggcaggtt cttgacggcg cgaagccccg cgacggtccc gatcaccacc
     3241 tcgccgggcc tcttctcgag gccgggtatc tcctgcgacg gcggcaaggc gtagcgcggc
     3301 acgtcgatgc cgttggcgat ccgggcgacc cgctccgccg gctggcccca ggccttgcgc
     3361 gcgatcgcct cgagccgggc cgagggcacc accagccggt gggcggcgcc gaggccgagg
     3421 cgccggtaga ggttgcgccg ggtcttcagc tcgccggcct cgtcctcgtt gaagccgtcc
     3481 tcgtggtgga cgagcggggg accgccgaac agatgccgcg ccatcaccgc gtcgaacgcg
     3541 ccccaattgt aggtgaggac gaggtcgaag cggcgcatgt agcgcgacag ccggaacagg
     3601 cgcggcagcc cgggcaggcc ggacaatggc ggcgcgtcgg ccgggaaatc cgccgcgact
     3661 tcgggcgcga gcgcctcgcg ggcgccgaac tcgccgcgcg acgcggatat gacggcatgc
     3721 tcggcggagc cgccgaatgc gttcatcagc cggaccgccc gcgcttcctt gccgccgcgg
     3781 ccgaaggtgg aatgggcatg gaggatgcgc accgcgctca tcgcgccagc atggcaggtg
     3841 agcgccctcc ccgtcaccct cttcgtcacg ccgaagcgag ttggcgctta tcctcttacg
     3901 ccaagggtgc ctagatgttg taagcggaat cgatgtggaa gctcgttcac cgattcctct
     3961 gttggctccc cttcggcgtc gctggcagcg tttgggccat cacagagcgc tggcccaagg
     4021 agtcccagcc ttgggtggat tggacgctcg gcgtgctgag cagctggccc gctgggctcg
     4081 cagtagggct gttctcggtc gcttatattg cggcgataat ttggactggc attgagcctc
     4141 ggaaagtggc ggctcccttt gagccgcctc ctatgcccac gctgaccccg gcggaagcgg
     4201 ttatctacga gcgaacgaga cttgctcaaa tgcgcgccga agagcaacga gccttagacg
     4261 cggaggaaca ggcgaggagt agccgcgagt ttcatcgcgc ccttgatgct tacacgcgat
     4321 tcgagaggag ggcacctcca cccttgccta gcgtcggtca agcgcatctt ggtacgccgg
     4381 gagccgccgt gttcgggata gatgcggcgg agtcgttcgt agcaatcgac cttcagaatt
     4441 tcggccaaac tccggcccga gacgtcacct acaagacatt gaccggatgg tttccccaac
     4501 agccgaggga accaatcgcc tatgaatcag agggcggcgt cggtatcacc gatccggggc
     4561 acgtccagca cattaagctg atcttgaaag gtccactgag gagcgccctc attcggcgta
     4621 gcgtgcgagg aggagagggt ttctacgttc tcgcaagcgt cgattacact accgagaacg
     4681 gcacgaggaa gcggcgcaag attgcctatt acttagacct tcaagggcag caacttccgg
     4741 acaggtggat gctcagtgtg tgccctacag ggaacgatga acttgatctg ggtatcggtg
     4801 atccgacagc ctacggcgac catgaaccgg caagggacgt ggagcgaaca gagcgtcgtc
     4861 gcgccctgat taagatgggt cgggacttgg cgttcggcta ctgtcacggc gaccgagagg
     4921 agacctttcg cgagtaccta gagggtcagc gcggctacgc tgatattcga ccacacttga
     4981 gcgagggcta caggggcagg ctggaggcgg ttcggatggt ctacgccgat ccggacggcg
     5041 cgaaataccc gaccctcgtg caatggtttc tggacgagct ggatcggctc gaaatggaat
     5101 ggggcctgat atgacgaaag accctcgcgc ccgcttctac cgccttttca aggcggtgct
     5161 ctccgtcccc cgcctagcgc gcgaaagcgc agcaggcagc cgcagaacac gaaggagcag
     5221 ccttggcgca gggaataaac ccgaaggaag ttcaggacgg cgtcagcgct ggaggcgaac
     5281 tactccgcgg cgatctgata ttgctccgcc ggcgcgagca ggcgatcgaa cgcgtcgaca
     5341 tgggccccat agtccgccac gtgccgccgc cagcaggtga caggctccgg cggcctcgag
     5401 gccggaacgg cgatcggcgg tatcgagcgc ggccgcgccg tcggccggtc gaccaccatc
     5461 tggtggaacc actcgcccgc atagctgccg tcgaaccgat gcacccgcga gccgagcacc
     5521 cggcccagcg gcacgccgtc gaggccgaag atcgtcgtct cgaacaggaa gccccgcggc
     5581 tcgccggcgg tgctgtacac gtacatcgtc attccaaccc tgcccctcgc tacccgatcc
     5641 ggccccgcct cccggcggca ccgcccgccc aaaggttcgc cgggacccgc cggccgttca
     5701 agctcgtaca aacccgtagt taacccgttg ccgctaagcc tcgactggac attcggggag
     5761 cgcggccgat cgtgaagctg atcgtgatga agaagggcgg cgagccgggc gaggtggcca
     5821 tcaaccccga tcgggtgacc catgtgcgaa ccgcctcggg gccgttcacc gacatctatt
     5881 tcggcgatca tcgggtcgcg gtcgaaggca cgttcgccca ggtcgtcgcg cggctcaccg
     5941 gccaggccga gatacccgat gccccgcccc agaagagctg gttccccgcc cgctgacggc
     6001 gaagcgcggt cctacaggat gaagtcggag cccagcatcg cggacgagtc ggtgcgggtg
     6061 accgagatca tcaagtcggc ctgggtcgag aagtcggcgt tggcgtcgcc gtcgacgtcg
     6121 gccatgatca gccactggtt catgcccatg tcgacgatcc tgagctcgcc ggcgtggccg
     6181 gtgaactggg cgctgccgat gacggtgaag gcctggtcgc cgggcgtcgt gatgtcggcg
     6241 tcgatccggc tgaggtcgat cttgtcgacg ccgccctgga agtcctggat gctgtcgccc
     6301 tgccagggca gggattcgcc ggccgcgcga agctggaacg tgtccgctcc gccgccgccg
     6361 gtgagggtgt cccggccgcc gccgccgtag agcaggtccg cccccgctcc gccgatcagg
     6421 gtatcgttgc cggcgccgcc gtacatggtg aggctgccgt ccaccacggc gctgccgttc
     6481 acgctgagag tctgggtggg gtcgaccagc gaatttgcgt tgacgatcag gttcccgccc
     6541 ggggtcacat tgccgttgtg cagcacgaag gcgtaggacg gcttggcgct cggatcgctg
     6601 gccagcgtcg cgttgaccga gatcgactcg atgttggtca ggctgttggc tccgaacgtc
     6661 acctgcttga ggccggtgcc gctgctcacc accgcgtcgc gcccgccgcc gccgtcgacc
     6721 cggtcgtccg ccccccaccg gtcgccctcg aagaagaaga cgtcgacccc cgctccgccc
     6781 ttgaggatgt cggtgccgtg gccgccatag acgaggaact tgccgtccgt ctcggccgag
     6841 ccgtcgaacg tcaggtcctc ccccacccg
//
LOCUS       DASVQZ010000085        10287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_787148, whole genome shotgun sequence.
ACCESSION   DASVQZ010000085 DASVQZ010000000
VERSION     DASVQZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787148"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VF547_02330"
     CDS             complement(<1..897)
                     /locus_tag="VF547_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8261690.1"
                     /translation="MQAMWRNYLTVGYRALTKSRTYAFINIVGLAIGLAACLMILLYV
                     RHETSYDKWLPDADRVFQVQSIHTDPETGQVSRQQGTHGVITESLAKDFPQIEAIARA
                     EGAEPVFLRNGEASFAPMIRADESFFQILDIPFLYGDKATALKNVDDLVLSRSEALKR
                     FGGINVVGQTMTVIRRGERSAMRVTGIFEDLPRNSHMNFKMVSRISDLEKGECGWGCI
                     NGNVYLKLRPGADPEEINRQLPAWEKRNITPIDVGGVKVSEGDEFDWKLVNVQDVHLS
                     EAEGEPERPGNDRGTLLTFTIVA"
     gene            complement(1043..1426)
                     /locus_tag="VF547_02335"
     CDS             complement(1043..1426)
                     /locus_tag="VF547_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX8261691.1"
                     /translation="MSENEGARKFQEAWGYSDAVVVGDTIYLSGIVVGLGRGETDLEA
                     AYERAYQRIGSILKRAGASWDDVVDISSFHTDVEGQIEKMVAAHKRHVKAPYPAWTAI
                     GVAKILGGGSTEIKIVAKRPGGAQR"
     gene            complement(1669..2349)
                     /locus_tag="VF547_02340"
     CDS             complement(1669..2349)
                     /locus_tag="VF547_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8261692.1"
                     /translation="MLSMRELSRVYRTDTVETTALDRIDLDIAEGEFVAIMGPSGCGK
                     STLLNVVGMLDSPTSGSYVFDGTEVAGLPESKLADFRKKNIGFIFQSFNLVDELSVRE
                     NIELALLYHDVPASERRSRVEAVMDKVGVAHRAKHRPSQLSGGQQQRVAVARALVGNP
                     KLILADEPTGNLDTQHGEEVMKMLQALNREGSTIVMVTHSPAHADYAGRVVNMLDGRI
                     LIERRRAA"
     gene            complement(2367..3671)
                     /locus_tag="VF547_02345"
     CDS             complement(2367..3671)
                     /locus_tag="VF547_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8261693.1"
                     /translation="MIRIKSNENGPRAESGTGMDKVVASTGLSQRTKIAIGVAAVLLL
                     GLLFWWFAPSAGSQTVAAERLTISVAAKGTFDDFLPLRARVEPLVTVYLDAVEGGRVE
                     KVMVEDGAIVQQGQLLAVLSNSDLALNLLARQSDVSREINSMRSQELALAQTSIGDER
                     AVIDAQLAADKARRQYEMQKPLVEKGFIPAKVFNDSRDEYLSNQRRAEVLRRGRAINQ
                     RLQTSQLAQLRASNASLSGSLDIARATLDALNIRAPVSGQLTAFSIQVGQSLNRGERL
                     GQVDSAGRNKLVASVDEYYLGRVQAGQTATVESGGKTYRVKVAKIYPQVRNGVFEVDL
                     IFVGAEPPALNRGQTLQIKLTLGDPAPALLIPNGAFYNETGGAWVFVVAPDGGEAVKR
                     NVRLGRRNADYIEVLEGLEPGERVITSPYTGFADKDRLDLET"
     gene            complement(3797..3976)
                     /locus_tag="VF547_02350"
     CDS             complement(3797..3976)
                     /locus_tag="VF547_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261694.1"
                     /translation="MRKMETWLTGLLWAAAALLLPMAALEPVIPASPQAVDVALGACA
                     DGSAHLAMGCASIQL"
     gene            4192..5595
                     /locus_tag="VF547_02355"
     CDS             4192..5595
                     /locus_tag="VF547_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8261695.1"
                     /translation="MAGERKPFWRCIVIDDDQDILLSARLLLRDMFEEVATFQAPEDA
                     MAAIGGDGADVVLLDANFGRGATNAAEGFHWLGEILARDPQAVVVMITAHGGVNIAVE
                     AMKRGATDFVSKPWSNERLLATVRTAASLRTSRQAAVTERKRAVAISAPAAGGETPLL
                     GQSAAMRRVYSLIERAAPTEANVLILGENGTGKELVARELHGKSRRAGNVFVSVDLGA
                     VAENLFESELFGHVKGAFTDARGDRIGRLQAADGGTLFLDEVGNLPLHLQPKLLTALE
                     QRQIVPVGANKPVAIDVRVIAATNLPPERLIDEGLFRQDLLFRLNTVEIELPPLRERR
                     DDIRVLVEHYVALYAKKYAKPARELPAEVMDALVAYDWPGNVRALRHAAERAVILAGE
                     GAFTFEDFSLSRSTAPRQAAVSTPPSLGADLNLERAEKQMVEQALKRHAYNISLAAQE
                     LGLTRASLYRRMDKHGL"
     gene            5585..6925
                     /locus_tag="VF547_02360"
     CDS             5585..6925
                     /locus_tag="VF547_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8261696.1"
                     /translation="MGFRSRFIAGLGWRAAMLLATLFLFLSALEVEGLGVARLLAAGL
                     GLWALYELWRYIQRTNMELARFLEAVRLGDLSQSFAHRADGSGFTDIGEALDEGIRSL
                     REERLRLTESNRFCEAVLDDAPAPLLTVDADGRVELANKTARRLFVRHHGVRIEDYRI
                     YGETFADALGDDSIGKPRLVPLMLDELPQTAMVSAASVHRLGGMVRVVAVQPIQGELN
                     AVEIAAQSDLVRVLTHEIMNSMTPVTSLAQSAASLMAEVDKGGDPAIADARAAVETLA
                     RRADGVMHFVESYRQISRTPVVRRRHFEVLPWARELESLFRAGDGADGVALSLEVTPE
                     RLTLDADPDLMCQVLINLLRNAAEAARDHSDSPAVSLAFAALPGARVQIEVADNGAGV
                     PEALRQDVFLPFFTTKAKGTGVGLSLARQVVLAHRGSIGLGTSDSGGALFRILL"
     gene            complement(6909..7409)
                     /locus_tag="VF547_02365"
     CDS             complement(6909..7409)
                     /locus_tag="VF547_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX8261697.1"
                     /translation="MLSQRTRYTIRALLHLADRYGEGPVQLSEIAQAQNIPPKFLTVM
                     LSQMIREKLVASRRGREGGYWLAKPPGEISYGAIVRLTRGSLGLLPCASRYRHETCRN
                     CISEDQCRLHRVMLMVRDETARILDGLSLADAIPEDAFADGSFDTGLRPGSVPAPAAM
                     SPKAIS"
     gene            complement(7462..9393)
                     /locus_tag="VF547_02370"
     CDS             complement(7462..9393)
                     /locus_tag="VF547_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8261698.1"
                     /translation="MTALAGYWGFGGADQPSGRCERMLRAQQVYAPDPPVSWSGGDIA
                     VGRRLFRTLPEDRHDRGPVVGGGGRWVLVADLRLDNRPELCAELGIERARAALLSDAA
                     VAMAAVERWGDEAIGRLVGDFALALWDRDRARLLLARDFLGQRPLHYHRGSGFFAFAS
                     MAKGLHTLPELPPEPDCRTVAAFVALMPDNGPETFYAGVEKVLAGHVVTVTRDGLRSE
                     RWWRPAMRDLGLKGTEQYAAALREQFDRAVADRLRGAEAGIASHLSAGLDSSTVTATA
                     ARLLAPSGARLTAFTAVPRQGFPERSSAEVIPNEGPHAAAVAAMYPNIDHVLVPNSGS
                     PVASLDRNFFLCERPVLNLCNSVWSNRIQDLARERKLGILLTGARGNASISYDGMALL
                     SELASSGRLLRLARESWALVRNGTRVGSVAAQTIGPFLPGLLWRAISRLRGRGRRLTD
                     YTVINPEAIEAMRLRELAAERGLDPNYRPRRDAFEARVWALGRTDIGNYNKATLGGWG
                     IDMRDPTADQRLIEFCLSVPLEEYLRDGRRRALVRTAFADRLPPVIVEEKRKGHQAAD
                     WYEGVNAARDAIAEELGRIAASAPARTALDSRKMERLVGDWPTEGWQRQATVDNYRLA
                     LMRGLSAGHFVRKASGSNE"
     gene            complement(9397..9669)
                     /locus_tag="VF547_02375"
     CDS             complement(9397..9669)
                     /locus_tag="VF547_02375"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEX8261699.1"
                     /translation="MIVSRRAELIETEVDGELVALHVDNGTCYGFNGTATRIWAMIEQ
                     PRRLSELKAELTREFDVDPEVCERQLGELLRELEGDGLVELAPAAD"
     gene            complement(9769..9909)
                     /locus_tag="VF547_02380"
     CDS             complement(9769..9909)
                     /locus_tag="VF547_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261700.1"
                     /translation="MQDERAKAPQPQHERATWIEPEISRLEAGSAELQIGQIDDGPND
                     KS"
     gene            complement(10007..>10287)
                     /locus_tag="VF547_02385"
     CDS             complement(10007..>10287)
                     /locus_tag="VF547_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HEX8261701.1"
                     /translation="VSGSADKILDFSIGDLIDLSVIDADSGTAGNQAFSFIGNAAFGN
                     HAGELRYQSNGGNEWLVQGDTNGDGTADFQFVLVINDADPITVADFIP"
BASE COUNT         1498 a         3549 c         3561 g         1679 t
ORIGIN      
        1 agcgacgatg gtgaaggtga gcaaggtgcc gcggtcgttg ccgggtcgct cgggctcgcc
       61 ttccgcttcg ctgagatgga cgtcctggac gttgaccagc ttccagtcga attcgtcgcc
      121 ctcgctgacc ttcacgccgc cgacgtcgat cggcgtgatg ttgcgcttct cccaggcggg
      181 gagctgccgg ttgatctcct cgggatcggc gcccggccgg agcttgagat agacgttgcc
      241 gttgatgcag ccccagccgc actcgccctt ctcgaggtcg ctgatccggc tgaccatctt
      301 gaagttcatg tggctgttgc gcggcaggtc ctcgaagatt ccggtgaccc gcatcgccga
      361 gcgctcgccg cggcggatga cggtcatcgt ctggccgacg acgttgatcc cgccgaaccg
      421 cttcagcgcc tcgctgcggc tcagcacgag gtcgtccacg ttcttcagcg cggtagcctt
      481 gtcgccgtag aggaagggga tatcgagaat ctggaagaag ctttcgtcgg cccggatcat
      541 gggcgcgaag ctcgcctcgc cgttgcgtag gaagaccggc tcggcgccct cggctcgggc
      601 gatcgcctcg atctgcggga aatccttggc gagcgattcg gtgatgacgc cgtgggtgcc
      661 ctgctggcgg ctgacctggc cggtctcggg gtcggtgtgg atgctctgga cctggaagac
      721 ccgatcggcg tcgggcagcc atttgtcgta gctggtctcg tggcggacgt agaggaggat
      781 catcaggcac gccgccaggc cgatcgcgag gccgacgatg ttgatgaagg cgtaggtccg
      841 gctcttcgtc agggcccggt agccgacggt gagatagttg cgccacatgg cttgcatcag
      901 atcctgcccg ccctctcccc ttcaggggag aggagaagcc gccgaaggcg gcgaaggaga
      961 gggggagtcg cgcgggtctc gccccactcc ccctctcccc ggccctctcc cctgaaggtg
     1021 agagggaggt cgtcgtggcc ggtcatcgct gcgcccctcc gggccgcttc gcgacgatct
     1081 tgatctcggt ggatccccca cccaggatct tggccacgcc gatcgcggtc caggccgggt
     1141 agggcgcctt cacgtggcgc ttgtgggcgg cgaccatctt ctcgatctgg ccttcgacgt
     1201 cggtgtggaa gctgctgatg tcgacgacgt cgtcccagct cgcccccgcc cgcttcagga
     1261 tcgagccgat gcgctggtag gcccgctcat aggccgcctc gaggtcggtc tcgccccggc
     1321 ccaggccgac gacgatgccg ctgaggtaga tcgtgtctcc gacgacgacc gcgtcgctgt
     1381 aaccccacgc ctcctggaac ttacgcgcgc cctcgttctc gctcatcagc accgtcgagt
     1441 cctgccggac cccggctccc gccgcggcgg ccgggacgat ggccgccgcg acggaaagga
     1501 tgaggaggag gaagcgccga aattcttccc cgagatttct cggggagggg gaccaggcga
     1561 agcctggtgg aggggttctt gctgagtccg gaaagaaccc ctccgcggcg cttcgcgcgg
     1621 tccccctccc cacgaaatcg cggggaggga ttgaggcgcg gaaagctgtc acgccgcgcg
     1681 gcgccgctcg atgaggatcc ggccgtcgag catgttgacg accctgccgg cataatcggc
     1741 atgcgccggc gagtgggtga ccatcacgat ggtcgagcct tcgcggttca gcgcctggag
     1801 catcttcatc acttcctcgc catgctgcgt gtcgaggttg ccggtcggct cgtccgccag
     1861 gatcagcttg ggattgccga ccagggcgcg ggcgacggcc acgcgctgct gctggcctcc
     1921 ggacagctgg ctcggccggt gcttggcgcg gtgggcgacc ccgaccttgt ccatcactgc
     1981 ctcgacccgc gagcggcgct cggaggccgg gacgtcgtga tagagaaggg ctagctcgat
     2041 attctcgcgc accgacagct cgtcgaccag attgaagctc tggaagatga agccgatgtt
     2101 cttctttcgg aaatcggcga gcttgctctc cggaagcccg gccacctcgg tgccgtcgaa
     2161 cacgtaggag ccgctggtcg gcgaatcgag cattccgacg acgttgagca aggtcgactt
     2221 gccgcagccc gaggggccca tgatggcgac gaattcgccc tcggcgatgt cgaggtcgat
     2281 tcggtccagg gcggtcgtct cgacggtgtc ggtgcggtag acccgcgaca gttcacgcat
     2341 gctcagcatt gcttccagct ccttcgtcag gtctcgaggt ccagccgatc cttgtcggcg
     2401 aaaccggtat agggagaggt gatgactcgc tcgccgggct ccagcccctc gagcacctcg
     2461 atatagtcgg cgttgcggcg gccgaggcgg acgttgcgct tgaccgcctc gccgccgtcc
     2521 ggggcgacga cgaacaccca cgcgccgccg gtctcgttgt agaaggcgcc gttggggatg
     2581 agcagggccg gcgccgggtc gccgagcgtc agcttgatct gaagcgtctg gccgcggttg
     2641 agcgcgggcg gctccgcgcc gacgaagatg aggtcgacct cgaacacccc gttcctgacc
     2701 tgcgggtaga tcttggcgac cttgacccgg taggtcttgc cgccggactc gacggtcgcg
     2761 gtctggcccg cctggacgcg gccgagataa tattcgtcga ccgaggcgac gagcttgttg
     2821 cggccggcgc tgtcgacttg gccgagtcgc tcgccgcggt tcagcgactg gccgacctgg
     2881 atcgagaagg ccgtgagctg gcccgacacg ggcgcccgga tgttgagcgc gtcgagggtg
     2941 gcccgggcga tgtcgaggct tccgctcagc gaggcgttgg aggcacggag ctgcgcgagc
     3001 tggctggtct gcagccgctg gttgatggcc cggccccggc gcagcacttc cgcgcgtcgc
     3061 tggttggaca gatattcgtc gcggctgtcg ttgaacacct tggccgggat gaagcccttc
     3121 tccaccagcg gtttctgcat ctcgtactgg cgccgggcct tgtccgccgc gagctgcgca
     3181 tcgatgaccg cgcgctcgtc gccgatgctg gtctgggcga gggcgagctc ctggctgcgc
     3241 atcgagttga tctcgcgcga cacgtcggac tgccgcgcga gcaggttgag cgccagatcg
     3301 gagttggaaa ggacggcgag cagctgcccc tgctggacga tggctccgtc ctcgaccatc
     3361 accttctcga cccggccgcc ctcgaccgcg tcgagataga ccgtgacgag cggctcgaca
     3421 cgcgcccgca agggcaggaa gtcgtcgaac gtgcccttcg ccgccaccga gatggtcagc
     3481 cgctccgccg ccacggtctg gctgcccgcg ctcggcgcga accaccagaa gagaaggccc
     3541 agcagcagca ccgccgcgac gccgatggcg atcttggtcc gctgcgacag gcccgtggag
     3601 gccacgacct tgtccatgcc ggtgccgctc tcggccctcg gcccgttctc gttcgatttg
     3661 atcctgatca cgctcatccc aacctcatgc gagtccatca gcaatcatcg tgccacggac
     3721 aggacggcgg cgcaggccgc cgggcgccga cgagtgtacg attccggaca tacgcctgta
     3781 cgggggcgga caggggtcac aattgtatgc tcgcgcaacc catggcgaga tgcgcgctgc
     3841 cgtcggcgca ggcgccgagc gcgacgtcga cggcctgcgg gctcgccggg atcaccggct
     3901 ccagggccgc catcggcagc agcagcgcgg ccgcggccca cagcaggccc gtcagccatg
     3961 tctccatctt ccgcatcgtc tgtctcccgg atactcgccc actgctcagc atgagccgtg
     4021 ccaatggcgg aaaacggcgg tttccggcga ttatcggcgg ctgtgccagg tcccgtggcg
     4081 ttcgcttccg aacgccgcga gtccgtggcc ggacgatccg gccgcgctcg gcggcaccat
     4141 tggcacgatt attgtttgtc cggttccgga cgccgctgta ggaggaacgc tgtggctggg
     4201 gagcgtaagc cgttctggcg gtgcatcgtc atcgacgacg atcaggacat attgctctcc
     4261 gcccggctcc tcctgcgcga catgttcgag gaggtggcga ccttccaggc ccccgaggac
     4321 gcgatggcgg cgatcggcgg cgacggcgcc gacgtcgtct tgctcgacgc caatttcggc
     4381 cgcggcgcga ccaacgccgc cgaaggcttt cactggctgg gcgagatcct cgccagggat
     4441 ccgcaggcgg tggtggtgat gatcacggcc catggcgggg tcaacatcgc cgtcgaggcg
     4501 atgaagcggg gcgccaccga tttcgtctcc aagccctggt ccaacgagcg tctgctggcg
     4561 acggtccgaa ccgccgcgtc gctgcggact tcgcgacagg cggcggtcac cgagcgcaag
     4621 cgcgcggtcg ccatctccgc ccccgccgcc ggcggcgaga cgcccctgct cggccaatcc
     4681 gccgcgatgc gccgggtcta ttcgctgatc gagcgcgccg cgccgaccga ggcgaacgtc
     4741 ctcatcctcg gcgagaacgg caccggcaag gagctggtgg cgcgcgagct ccacggcaag
     4801 tcgcggcgcg ccggcaacgt cttcgtgtcg gtcgacctgg gcgcagtggc cgagaatctg
     4861 ttcgaatccg agctgttcgg ccatgtcaaa ggcgcgttca ccgacgcccg cggcgaccgc
     4921 atcggccggc tgcaggcggc cgacggaggc accctcttcc tcgacgaggt cggcaatctt
     4981 ccgctccacc tccagccgaa gctgctgacg gcgctggagc agcggcagat cgtgccggtc
     5041 ggcgccaaca agccggtcgc gatcgacgtc cgggtgatcg cggcgaccaa cctgcccccg
     5101 gagcggctga tcgacgaggg cctgttccgc caggacctgc tgttccgcct caatacggtc
     5161 gagatcgagc tgccgccgct gcgcgagcgc cgcgacgaca ttcgggtgct ggtggagcat
     5221 tacgtcgcgc tctacgcgaa gaaatatgcc aagcccgccc gcgagctgcc ggccgaggtg
     5281 atggacgctc tggtcgccta cgactggccg ggcaacgtcc gggcgctccg ccacgccgcg
     5341 gagcgcgccg tcatcctcgc cggcgaagga gccttcacct tcgaggattt ctccctgtcc
     5401 cgctcgacgg cgccccgcca ggccgcggtc agcacgccgc ccagcctcgg cgccgacctc
     5461 aacctcgagc gcgccgagaa gcagatggtc gagcaggccc tgaagcggca cgcctacaac
     5521 atctcgctgg ccgcccagga gctcggcctg acccgagcct cgctctatcg acgcatggac
     5581 aagcatgggc tttagaagcc gcttcatcgc cggcctcggc tggcgggcgg cgatgctgct
     5641 tgcgacgctc tttctgttcc tgtcggccct ggaggtcgag ggcctgggcg tcgcccgcct
     5701 gctcgcggcc ggcctcggcc tctgggcgct ctacgaattg tggcgctaca tccagcgcac
     5761 caacatggag ctcgcccgct tcctggaggc ggtgcggctg ggggatcttt cgcaaagctt
     5821 cgcccatcgc gccgatggca gcggcttcac cgacatcggc gaggcgctcg acgagggcat
     5881 ccggagcctg cgcgaggagc gcctgcggct caccgaatcg aaccggttct gcgaggcggt
     5941 gctcgacgac gccccggctc cgcttctgac cgtcgacgcc gacggccggg tcgagctggc
     6001 caacaagacg gctcggcggc tgttcgtccg ccaccacggc gtcaggatcg aggactaccg
     6061 gatctacggc gagaccttcg ccgacgcgct cggcgacgat tcgatcggca agccgcggct
     6121 ggtgccgctg atgctggacg agcttccgca gacggcgatg gtcagcgccg cctcggtcca
     6181 ccgcctcggc ggcatggtgc gcgtagtcgc ggtccagccg atccagggcg agctcaatgc
     6241 ggtcgagatc gccgcccaga gcgacctcgt ccgggtcctg acccacgaga tcatgaactc
     6301 gatgaccccg gtgacgtcgc tcgcgcagag cgcggcgagc ctgatggccg aggtcgacaa
     6361 gggcggcgat ccggcgatcg ccgacgcccg cgcggcggtc gagactctcg cccggcgcgc
     6421 cgacggggtc atgcatttcg tcgagagcta tcgccagatc agccgaacgc cggtcgtccg
     6481 ccgccgccat ttcgaggttc tgccctgggc gcgcgagctc gaatccctgt tccgggccgg
     6541 ggacggcgcc gacggcgtcg cgctgtcgct cgaggtgacg cccgaacggc tgacgctcga
     6601 cgccgacccc gacctgatgt gccaggtgct gatcaacctg cttcgcaacg ccgccgaggc
     6661 cgcgcgcgac cacagcgact cgccggcggt gtcgctcgcc ttcgccgccc tgcccggcgc
     6721 gcgagtccag atcgaggtcg ccgacaacgg cgccggcgtg cccgaggcgc tgcgccagga
     6781 cgtgttcctg cctttcttca ccaccaaggc gaagggcacg ggcgtgggcc tcagcctcgc
     6841 ccggcaggtg gtcctcgccc atcgaggctc gatcggactc ggcaccagcg acagcggcgg
     6901 cgcgctgttc aggatattgc tttagggctc atcgctgccg gagcggggac cgagcctggg
     6961 cgaaggccgg tgtcgaagga cccgtcggcg aaggcgtctt ccggtatcgc atccgccagc
     7021 gacagcccgt cgaggatccg cgcggtctcg tcccgcacca tcagcatcac ccggtgcagc
     7081 cggcactggt cctcgctgat gcagttgcgg cacgtctcgt ggcgataccg gctcgcgcag
     7141 gggagcaggc cgagcgagcc gcgggtgagc cgaacgatcg cgccatagct gatctcgccg
     7201 ggaggcttcg ccagccaata gccgccctcg cgcccgcgcc ggctggcgac gagcttctcg
     7261 cggatcatct gcgaaagcat caccgtcagg aacttgggcg ggatgttctg ggcctgggcg
     7321 atctcgctga gctggaccgg tccttcgccg tagcggtcgg ccaggtggag gagcgcgcgg
     7381 attgtatagc gcgttcgttg agaaagcatt ccgcctcatg aaccctggcg acaggcttgg
     7441 caaccgggcg gcggcgtcgg gctattcgtt cgagccggac gccttgcgga cgaagtgacc
     7501 ggcggacagg ccgcgcatca gcgccagccg gtagttgtcg accgtggcct ggcgctgcca
     7561 gccctccgtc ggccagtcgc cgaccagccg ctccatcttc cgcgagtcga gcgccgttcg
     7621 ggccggggcc gaggcggcga tccggcccag ctcctcggcg atggcgtcgc gagcggcgtt
     7681 caccccctca taccagtccg ccgcctggtg gcccttgcgc ttctcctcga cgatcaccgg
     7741 cggcagccgg tccgcgaagg cggtccggac cagcgcgcgc cgccggccgt cgcgcagata
     7801 ttcctcgagc ggcaccgaca ggcagaattc gatcagccgc tggtcggcgg tcggatcgcg
     7861 catgtcgatt ccccagccgc ccagggtcgc cttgttgtaa ttgccgatgt cggtccggcc
     7921 cagcgcccac acccgggctt cgaaggcgtc ccgccgcggc cggtaattgg ggtcgagccc
     7981 gcgctccgcg gcgagctcgc ggagccgcat ggcctcgatc gcctcggggt tgatcacggt
     8041 atagtccgtc agcctccgcc cccgcccgcg caggcggctg atcgcccgcc agagcagccc
     8101 gggcaggaaa gggccgatgg tctgggccgc gacgctgccg acccgggtgc cgttgcggac
     8161 gagggcccag gactccctcg ccaggcgaag cagccgtccg ctcgaggcga gctcggagag
     8221 gagggccatg ccgtcgtagc tgatgctcgc attgccgcgg gcgccggtga gcaggatgcc
     8281 gagcttgcgc tcgcgcgcga ggtcctggat ccggttgctc cataccgaat tgcagaggtt
     8341 gaggacgggc cgttcgcaga ggaagaagtt ccggtcgagg ctggcgacgg gcgacccgct
     8401 gttggggacg agcacatgat cgatattggg gtacatggcc gccaccgccg ccgcatgagg
     8461 cccttcgttc gggatgacct ccgccgacga gcgctccgga aacccctggc ggggcacggc
     8521 ggtgaacgcc gtcaggcgcg cgccgctcgg cgccagcagc cgcgccgccg tcgcggtgac
     8581 cgtgctgctg tccagccccg cgctgaggtg ggaggcgatc ccggcctcgg cgccgcgcag
     8641 ccggtccgcg accgcccggt cgaactgctc gcgcagggcc gcggcatatt gctccgtccc
     8701 cttcagcccc aggtcgcgca tcgccggacg ccaccatcgt tcggagcgca gcccgtcgcg
     8761 ggtcacggtg acgacatggc cggcgagaac cttctcgacg cccgcgtaga aggtctcggg
     8821 cccgttgtcg ggcatcagcg cgacgaacgc cgccaccgtc cggcaatcgg gctcgggcgg
     8881 aagctcgggc agggtgtgaa gccccttggc catggaggcg aaggcgaaga agcccgagcc
     8941 gcgatgataa tggagcggcc tctggcccag gaagtcgcgc gccagcagca gccgcgcgcg
     9001 gtcgcggtcc catagcgcca gggcgaaatc gccgacgaga cggccgatcg cttcgtcgcc
     9061 ccagcgctcc accgccgcca tggcgaccgc ggcgtcggag aggagggcgg ctcgagcccg
     9121 ctcgatgccg agctccgcgc agagctccgg ccgattgtcg agccgcaggt cggcgaccag
     9181 gacccagcgc ccgccgccgc cgacgacggg gcctcgatcg tggcggtcct cgggaagcgt
     9241 tcggaacagc cggcggccga cggcgatgtc gccgccgctc cacgaaacgg gcggatcggg
     9301 cgcatagacc tgctgggccc tcagcatccg ttcgcaacgg ccggagggtt ggtcggcgcc
     9361 gccgaacccc caatagcccg ccaaggcggt catggcttaa tcggcggcgg gcgcgagttc
     9421 gacgaggccg tccccttcga gctcccggag cagctcgccc aattgccgct cgcaaacctc
     9481 gggatcgacg tcgaactcgc gggtcagctc cgccttcagc tcggacaggc gccgcggctg
     9541 ctcgatcatc gcccagatcc gcgtggcggt gccgttgaag ccgtagcagg tgccattgtc
     9601 gacgtgcagc gcgacgagct ccccgtcgac ctccgtctcg atcagctccg cgcgccgact
     9661 gacgatcatg tcttgtgtca cgccgatgta tttccggttt ctgagggcga cgcgggggcg
     9721 acgcggacgc gcgtcgcgcc cgctccgccc cttcgcccgg tcgcggcttc agctcttgtc
     9781 gttggggccg tcgtcgattt ggccgatctg aagctcggcc gagcccgcct cgaggcgaga
     9841 gatttcgggc tcgatccagg tggcgcgttc gtgctgcggt tgcggcgctt tggctctctc
     9901 gtcctgcatg gtcgcttatc ctgcccttcg aatcactgtt gctgcctacc tctacaacac
     9961 caattcggcg ttggcgagag agaaggcccg gggtccgaac ggagccctag gggatgaagt
    10021 ccgcgaccgt gatcggatcc gcatcgttga tcacgagcac gaactggaag tcggccgtgc
    10081 cgtcaccgtt ggtgtcgccc tggaccagcc actcgttgcc gccgttgctc tggtagcgga
    10141 gctcgccggc gtggttgccg aaggcggcgt tgccgatgaa gctgaacgcc tggttgccgg
    10201 ccgtcccgct gtccgcgtcg atcacggaca ggtcgatgag gtcgccgatc gagaagtcga
    10261 ggatcttgtc cgcgcttccg gagacgg
//
LOCUS       DASVQZ010000086         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_787290, whole genome shotgun sequence.
ACCESSION   DASVQZ010000086 DASVQZ010000000
VERSION     DASVQZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787290"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..425
                     /locus_tag="VF547_02390"
     CDS             108..425
                     /locus_tag="VF547_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6127 family protein"
                     /protein_id="HEX8261702.1"
                     /translation="MMADPGNPMLALLVAQAEGQGAGMVTLRALVEEASELGAERALE
                     ALGLRDQKARRDMDELRELLQAWRDAKKSAWQAVVGWAVRILLALLVLGMSVKLGLWE
                     QLR"
     gene            422..970
                     /locus_tag="VF547_02395"
     CDS             422..970
                     /locus_tag="VF547_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEX8261703.1"
                     /translation="MSGASTPAKAGAQVQLPSGPRPSPGRSAVRFAGYAAVFDRPDRG
                     GDVVRPGAFARSLKRRARAVPLLWQHDVRRPVGRVEYLKEDRRGLRVIARLSDGEAGR
                     VAAALLEAGQVAGLSFGYWVRTASGQAPRLLTEVDLVEISLVTVPMQPGARVHALAHG
                     AQASSCGATMVKPGTGRSSGPS"
     gene            complement(904..1341)
                     /locus_tag="VF547_02400"
     CDS             complement(904..1341)
                     /locus_tag="VF547_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261704.1"
                     /translation="MTSKLLAALALLATAAGPAAGQGRPNEARDLMRSIGGGGLSGKK
                     LAKAIAKAERSPLGSKANPVRENMPTGELAYLARLRCPDGSKPAAERQGNVGMGVYRN
                     IVDLYRVTCAGAAPVEIYMDMYHDGPELRPVPGFTIVAPQDDA"
     gene            1516..2661
                     /locus_tag="VF547_02405"
     CDS             1516..2661
                     /locus_tag="VF547_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX8261705.1"
                     /translation="MWSEEGNDMLEVKADPLEASFEALEREGDEVAQLREEMAQLKSR
                     IDAQAIAGARPALSGARAQASPFVEGYLRKGLESGIEVKAMSGTTDSAGGYAVPEEID
                     AEIGRLLTAISPIRAIANVVKVGSAGYRKLVTSGGTPSGWVGETAPRPETGTPTFIEV
                     APPSGELYANPAASQAMLDDAAFDVEDWLASEIATEFARAEGSAFVAGSGINRPKGFL
                     AAPNSAQGDPVRAFGTLQYLATGTAGGLGTNAADKLIDLVQTLRPPYRQGAVFVMNSA
                     TASTIRKLKTSDGAFLWQPGLTAGQPDSLLGYPVVEAEDMPDIAVDSLSIAFGNFRVG
                     YLIAERTETQILRDPFTNKPFVHFYATKRVGGQVANSEAIKLLKFSA"
     gene            2797..3345
                     /locus_tag="VF547_02410"
     CDS             2797..3345
                     /locus_tag="VF547_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261706.1"
                     /translation="MIEGEALVLPPEAAGAAKGFLRVERPDEDGLIETLAAAAAELCE
                     AFIGQALLARGFVETMPASRAWQKLSRTPVRAITLVEALPAEGPAQTLAVGAYAIDID
                     AAGDGWVRLTEPGAARRIRVGYQAGVAGSWPDLPAALRHGILRLAAHLYMNRAEEGGR
                     AAAEPPRAVAALWRPFRRLRMR"
     gene            3357..3572
                     /locus_tag="VF547_02415"
     CDS             3357..3572
                     /locus_tag="VF547_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261707.1"
                     /translation="MFERLMARAGELADRAAQRRRAALAEALGEDAPDGVRVREEEDG
                     IAISGARLGLRYALEPGLRWILTGRRR"
     gene            3569..3964
                     /locus_tag="VF547_02420"
     CDS             3569..3964
                     /locus_tag="VF547_02420"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEX8261708.1"
                     /translation="MSGAGAALQAAAIEAVAALELGGAYPGPPLQAAFPHAIVECGAE
                     SDWGHKSGKGRELRLAITLRDSGERPERAQAFADVAEAAIEAGLDVDGWRLVSLALLR
                     RRTVAEARGSRAGWAVTIDYRARMLADEG"
BASE COUNT          567 a         1320 c         1641 g          567 t
ORIGIN      
        1 agtgactgcc accagtgaaa gtcacttctt ggcgattttt cgagggctcg gcgggttcgg
       61 gaggtggcgg gtctcgccgg gaccacggat cttttcgaag gacagcaatg atggccgatc
      121 cgggcaatcc gatgctcgcc ctcctcgtcg cccaggcgga ggggcagggg gcggggatgg
      181 tgactctgcg cgcgctggtc gaggaggcga gcgagctcgg cgccgagcgc gcgctcgagg
      241 cgctcggcct cagggatcag aaggcgcggc gcgacatgga cgagcttcgc gagcttctcc
      301 aggcgtggcg cgacgccaag aaatcggcct ggcaggcggt ggtcggatgg gcggtgagga
      361 tcctgctggc gcttctggtg ctggggatga gcgtcaagct cggcctttgg gagcagctca
      421 gatgagcggg gcgagcactc cggccaaggc cggtgcccag gtccaactcc cgtctgggcc
      481 ccggccctcg ccggggcgca gcgctgttcg gttcgcgggc tatgcggcgg tcttcgaccg
      541 gcccgaccgg ggcggcgacg tggtccggcc gggcgcgttc gcccgcagcc tcaagcggag
      601 ggcccgggcg gtgccgctgc tttggcagca tgacgtgcgc cggccggtcg ggcgggtcga
      661 atatctgaag gaggaccggc gcggcctcag ggtgatcgcc cggctttcgg acggggaggc
      721 gggccgagtg gcggcggcgc tgctggaggc ggggcaggtg gccgggttga gcttcggcta
      781 ttgggtgagg accgcgagcg gccaggcgcc gcgcctgctc accgaggtcg atctggtcga
      841 gatcagcctg gtgacggtgc cgatgcagcc gggcgcgcgc gtgcacgcgc tcgcgcacgg
      901 ggctcaggcg tcgtcctgcg gagcgacgat ggtgaagccg ggaacgggcc gaagctcggg
      961 gccgtcgtga tacatgtcca tgtagatctc gaccggcgcg gctccggcgc aggtgacccg
     1021 gtagaggtcg acgatgttgc ggtagacacc catcccgaca ttgccctggc gttcggcggc
     1081 gggcttgctg ccgtccggac agcgcagtcg ggcgagatag gcgagctcgc ccgtgggcat
     1141 gttctcgcgg accggattgg ccttcgaccc gagcgggctg cgctcggcct tggcgatcgc
     1201 cttggcgagc ttcttgcccg agaggccgcc gccgccgatc gagcgcatca ggtcgcgcgc
     1261 ctcgttcggg cgcccctggc cggcggcggg gccggcggcg gtcgcgagca gggcgagggc
     1321 ggcgagcagc ttcgaggtca tggcgttcct cccgtgagca gcggggcagg atagaccgcc
     1381 ggcggcggaa gccaagggtg gagcgccgcg agcggggcag ccccttcggg cggacggccg
     1441 ccgccgccga ccgccgatcg agcgagacga attcgatttt ccggcttccg ccggaaggac
     1501 gggcacgcgc gtgccatttg gagcgaggag ggaaatgaca tgctggaagt gaaagcggat
     1561 ccgctggagg cgtcgttcga ggcgctggag cgggaggggg acgaggtcgc gcagctccgc
     1621 gaggagatgg cgcagctgaa gagccggatc gacgcgcagg cgatcgccgg cgcccggccg
     1681 gcgctgagcg gcgccagggc gcaggcctcg cccttcgtcg agggctatct gaggaagggg
     1741 ctggagagcg ggatcgaggt caaggcgatg tcgggcacca ccgactcggc cggcggctat
     1801 gcggtgccgg aggagatcga cgccgagatc ggtcggctgc tgaccgcgat ctcgccgatc
     1861 cgggcgatcg ccaacgtcgt caaggtgggc agcgccggct accgcaagct ggtcacctcc
     1921 ggcggcacgc cgtcgggctg ggtcggcgag acggcgccga ggccggagac cgggaccccg
     1981 accttcatcg aggtggcgcc gccgtcgggc gagctctacg ccaatccggc ggcgagccag
     2041 gcgatgctcg acgacgccgc gttcgacgtc gaggactggc tcgcgagcga gatcgcgacc
     2101 gagttcgcgc gcgccgaagg ctcggcgttc gtcgccggca gcgggatcaa ccggccgaag
     2161 ggtttcctgg cggcgcccaa ctcggcgcag ggggaccctg tccgggcgtt cgggaccctg
     2221 cagtatctgg ccaccgggac ggccggaggg ctcggcacga acgccgccga caagctgatc
     2281 gacctcgtcc agacgcttcg gccgccctat cggcagggcg cggtgttcgt gatgaactcg
     2341 gcgacggcgt ccaccatccg caagctcaag acttcggacg gggcgttcct gtggcagccg
     2401 gggctgacgg cgggccagcc cgacagcctg ctcggctatc cggtggtcga ggcggaggac
     2461 atgcccgaca tcgccgtcga cagcctgtcg atcgcgttcg gcaacttccg ggtcggctat
     2521 ctgatcgccg agcggaccga gacgcagata ctgcgcgatc cgttcaccaa caagccgttc
     2581 gtccatttct acgccaccaa gcgggtcggc gggcaagtgg cgaattcgga ggcgatcaag
     2641 cttctgaagt tcagcgcctg acgccgctcc cctccctgca cgcagggacg ggccgggggg
     2701 gggtgcgtcc gcggacgcac actgccgcgg aagcttgccc acccccaacc ccaccctgcg
     2761 tgcagggcgg gggatttctc ccaaggagag aatatcatga tcgagggcga ggcgctggtc
     2821 ctgccgccgg aggcggcggg ggcggcgaag ggattcctgc gcgtcgagcg accggacgag
     2881 gacgggctga tcgagacgct ggccgcggcg gccgcggagc tgtgcgaggc gttcatcggg
     2941 caagcgctgc tggcccgagg cttcgtcgag acgatgccgg cgagccgggc gtggcagaag
     3001 ctgtcgcgga cgccggtccg ggcgatcacg ctggtcgagg cgctgccagc cgaagggccg
     3061 gcgcagacac tggcggtcgg cgcctatgcg atcgacatcg acgccgcggg cgacggctgg
     3121 gtgcgactga ccgagcccgg cgcggcgcgg cggatccggg tcggctacca ggccggggtc
     3181 gccggcagct ggccggacct gccggcggcg ctcaggcacg gcatccttcg gctggccgcc
     3241 catctctaca tgaaccgcgc cgaggagggc ggccgggccg cggccgagcc gccgcgcgcg
     3301 gtggcggcgc tgtggcggcc ctttcgccgc ctgcggatgc gctgaaggag gatcgaatgt
     3361 tcgagcgact gatggcgcgc gccggcgagc tcgccgaccg cgctgcgcaa cggcggcgag
     3421 ccgcgctcgc cgaagccctg ggcgaggacg cgcccgacgg ggtgcgcgtg cgcgaggagg
     3481 aggatgggat cgcgatctcg ggagccaggc tcggcctgcg ctacgcgctc gagcccggcc
     3541 tgcgctggat cctcacgggg aggcggcggt gagcggcgcg ggcgcggcgc tccaggcggc
     3601 agcgatcgag gccgtggcgg cgctcgagct gggcggagcc tatcccgggc cgccgctgca
     3661 ggcggctttc ccgcacgcga tcgtcgagtg cggcgcggaa tcggattggg gccacaagag
     3721 cggcaagggc cgcgagcttc ggctggcgat caccctgcgc gactcgggcg agcggccgga
     3781 gcgggcgcag gccttcgccg acgtcgcgga agccgcgatc gaggccggcc tcgacgtgga
     3841 cggatggcgc ctcgtctcgc tcgcgctgct gcggcggcgc accgtcgccg aggcgcgggg
     3901 cagccgcgcc ggctgggcgg tgacgatcga ctaccgggcg cggatgctcg cggacgaagg
     3961 ctgacgagcc gcgttattgc ggcttgtcgg cgggaaggct gctcttgaac gtctcgatgg
     4021 cgttctcgtg gatgtagccg atctcttcct gagcgttgcg ctccgccgtc gcccgggcga
     4081 tcttggcgga gacgt
//
LOCUS       DASVQZ010000087        12401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_404413, whole genome shotgun sequence.
ACCESSION   DASVQZ010000087 DASVQZ010000000
VERSION     DASVQZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404413"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1472)
                     /locus_tag="VF547_02425"
     CDS             complement(51..1472)
                     /locus_tag="VF547_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8261709.1"
                     /translation="MTRKVQLSLSLAFAALAAAGCTNPDPPASASDQTPRARPATYSR
                     DPFPSTYRRYPGAVTAITGATVFDGEGGRIENGTVVLADGVVRAVGGPDTAIPAEATR
                     IDGRGKWVTPGIIDIHSHLGDYPSPGVEAHQDGNEVTGPVRSEVWAEHSVWPQDPGFS
                     RALANGGVTSLHVLPGSANLFGGRGVTLKNVPARTMQQMKFPDAPYSLKMACGENPKR
                     VYGGRNQMPASRMANIALMRQTWARAQEYRRKWDKYERDGGEMPGRDLAMDTLRGVLA
                     GEITVQNHCYRADEMANIMDMAKEFGYKVSTFHHAVESYKIADLLKANGVCSAMWADW
                     WGFKMEAYDGIQENIPFVHNAGACAIVHSDDANGIQRLYQEAAKALANGRRAGIDITD
                     EVAWTWLSLNPARALGIADKTGSLKPGKMADVVLWNGHPFSVYTRPERVWVDGALMYD
                     ANDPRRRPVSDFELGQPGEGDVK"
     gene            complement(1559..2542)
                     /locus_tag="VF547_02430"
     CDS             complement(1559..2542)
                     /locus_tag="VF547_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261710.1"
                     /translation="MALIETPPGNNHSGRRRFSQRALWPATILFEVPALANNQAGNAG
                     IELGSYPDWIVALTAIAATYLGLRQLRHLAEGQQAGVAAQQTAARHAEAAARHAEISK
                     QQAELTAQYGRSAAQQAKATARHAQVAARQAELAATQQRIVAEHHKENVQIAEANLLL
                     RIDETIEGPEMRPSRVALRTLFSSIDARWGHRTRSLSKSDYRCKIVSRYLDMLRHAAA
                     SAYAKDAQGRQRGEAAKARYDVLMLLPGYIETMGWLWRDGYISEERLLNLYDGIIVQV
                     IGSTLNHIRGRQGSNAHYLEMAIALYERALVWRDPLPQVWRDITLPPKKLR"
     gene            complement(2529..4379)
                     /locus_tag="VF547_02435"
     CDS             complement(2529..4379)
                     /locus_tag="VF547_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX8261711.1"
                     /translation="MVNVAPETTTEGEKPNVSRSDRDTWFGHPRQLARLFTTEMWERF
                     GYYGMRALLVLFLTQHFLFGDSTANGLYGAFTSLVYLTPLIGGLVADRYLGSKRSVKL
                     GAILMSLGYLGLCFNGPAAKPFFETGGQRYEVQIERQGEEATQYVIAGGQRYLFKGNE
                     DGSVNLTPAGAAPAAAGQAPAAPAAAAALPPSVAKGQYETGGERDPFFVMLMLFSLSA
                     VIVGNGLFKPNISTIVGSLYDATDSRRDAGFTIFYMGINLGSLISQFFCPLLAVWFGW
                     WAGFGLAAFGMLIAWALFQFDGGRLNGYGEPPAEHSHKTLMLVTLGALAMIPVAWFLL
                     NNTMANAAASAHAAQSGGGILGYIASLPILGQVMFWTYFAAVIGIPIWAYLKGTREEF
                     HMMVVAVILTVFSTVFWTLFEQAGSSLTLFADRNTDRQIGGYLMPAGQTQIFNPIFIV
                     LLAPLFSLMWVALARKRLEPSIPVKFAIGLTLVGAGFLVLVFGSQFAGGDYKVPLIWL
                     ALAYLIHSIGELCLSPVGLSMITKLSIARVVGLMMGVWFLSSAMAQYVGGVVAQFASV
                     ETVGGEVTNPALSLSTYMGVFKTIGLVAIGIGAFLFVFSFLLKKWMHGVN"
     gene            4504..5082
                     /locus_tag="VF547_02440"
     CDS             4504..5082
                     /locus_tag="VF547_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX8261712.1"
                     /translation="MLNDRTSLLSYLATRRSARPRDLVEPGPDPDQLRRILEIAARSP
                     DHGKLAPWRFVHVARDRRAAFAQTLEAAYRSGKAEPPSRIEIEAVHRFATQAPELIVL
                     LSCPVEGTKIPVWEQLLSCGAVGMNLLHAASALGFAGGWVTGWAAYSPAVLEALGGRE
                     PERVAGFFFLGTPGTELEERVRPALDDVVRQW"
     gene            5168..5512
                     /locus_tag="VF547_02445"
     CDS             5168..5512
                     /locus_tag="VF547_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8261713.1"
                     /translation="MSEERPVYLRLRDNIGAMILEGRVADGDPLPSVRSLAAEFGANP
                     LTVAKAYQSFQDEGLVLVRRGVGMFVAPGATDRLRKAGREDFLNHRWPEIVAQIRRLG
                     LSAEELLERVSA"
     gene            5792..6010
                     /locus_tag="VF547_02450"
     CDS             5792..6010
                     /locus_tag="VF547_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261714.1"
                     /translation="MHPARAFFTSLSISLVVLTLSYQAAALPAIIAPPKPVPMPASNA
                     WMDNAEAVKAKGRSQDDMSELAPLADVL"
     gene            complement(6150..7967)
                     /locus_tag="VF547_02455"
     CDS             complement(6150..7967)
                     /locus_tag="VF547_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8261715.1"
                     /translation="MDRRTFIASSGAAALAATLPATVLAQPRAGSGDAELNALFERIF
                     QQAVQRSPELATQLGLDKGEYAGLKGRLTPRTLAERARQLAQVKAALAELAAVDRSAL
                     SPAARLNLEVVTYSLTNQTIAPQRFGLDSAIRPYRIFQQGGAYFSVPDFLNTAHTINT
                     RDDAEAYLARLEAFAVALDQDSAMQRIEASRGYLAPAWSLDLTLGQMAKLREQKADES
                     SMVQSLVKRAKAKDIEGRWERRAERIVEERVYPALDRQMALVRDLRAASRPGDGIGRV
                     PHGDEIYAVALEQATTTRFTPDEVHRMGLQQVAEISAQLDRILRSQGMSRGSVGERLA
                     ALNVSPSQLYPNTDEGRAELIAGLNEGVKAMDARLPQAFATLPKAPLEIRRVPVEIQD
                     GASNGYYNRASLDGSRPAIYFINLKDTGDWPKYSLPALTYHEGSPGHHLQISLAQESA
                     DIPTIRKVGGFSAYSEGWALYAEQLADELGAYATPLERAGYLQSFLFRAARLVVDTGL
                     HAKQWSRERATDYLVATTGFARPRSQREVERYCTQAGQACSYKVGHMAWTRARTEAQR
                     ALGRRFDLKRFHEILKDGAMPLTILERRIRERTAGAKRG"
     gene            8268..8990
                     /locus_tag="VF547_02460"
     CDS             8268..8990
                     /locus_tag="VF547_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8261716.1"
                     /translation="MFGLYHQIALALFAGFVLAELLLPGRAFPTLRLWRLRAALSTIA
                     YFAVATFAPLIWDGTLGAYRLFDATALPFWAQVAGGFLALELGVYVWHRTMHNVDPIW
                     RWFHQMHHSAERIDVWGAFYFSPLDMLGFTFLGSLVLVLGFGLGGEAALVVNVFVTFL
                     AMFQHANIRTPHWLGYLIIRPESHAAHHERGVHAFNYCDLPLWDMLFGTFRNPREFEG
                     EAGFWDGASGRIGPMLLGRDVA"
     gene            8987..9877
                     /locus_tag="VF547_02465"
     CDS             8987..9877
                     /locus_tag="VF547_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261717.1"
                     /translation="MIRFLLAVLAAYMLVLGAAASAAPIDQGGWNARKQSVRLANGVR
                     LAYVELGDPDGAPLLLLHGYTDTSRSWTQVAPWLLRHRLLVPDQRGHGGSDAPACCYS
                     ASVFAEDARLFLDAMKVERAAVAGHSLGSMVAMTLAAEHPERVTRVALIGSTALVPVK
                     RGDWLWTNAHALKAPLDPSSDFLREWHPANQPTPVDKAFAEAAMAEILAIPLHVWRGV
                     MRELAEVPAGRHAADVGAPVLILSGGKDPLFSAEHHAALVKAFPKAESQVFPDLGHNL
                     NWERPADVGPVLARFFAAER"
     gene            complement(9982..12042)
                     /locus_tag="VF547_02470"
     CDS             complement(9982..12042)
                     /locus_tag="VF547_02470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8261718.1"
                     /translation="MRNFRLAFTAALLLGGAATPLLAQPGAAAPAAASAKPRYGDFGI
                     DLSAMDRSVKPGDSFWHYVNGNWDKTTEIAADRTSAGAGVLLVDEAERQVRAIVEDLA
                     RDPARSGKIGQQVGDFYASWMDEAAIEAAGTAPLKPYLARFAAISNRTDLIRAFAQPG
                     VISPVGVGILPDPADPTRYIAAAGQGGLGLPNRDYYLLQGEKYDAIRAAYRAYVVKIQ
                     QLAGIADAEAKADRIIALETALAKVHWEPARQRDIKQIYNPMNRAQLAELAPQLEWPT
                     FLDAAGLGSVETVIAAEKSAITETGKMLDSVPLSTWKDYLAFHFVSSHAPYLPKAFDQ
                     ANFDFYSKTLRGVPQMRDRWKRGVQIINGSLGEAVGRVYVERHYPPESNRQMAELIAN
                     LRSGLEARLAASTWMDDATKAQARAKLAAFDPRIGHPSKWIDYSSLDVKRGDLLGNVV
                     RSEKFAHELQLSRLGKPVDRALWDMTPQTVNAYYNPLMNQITFPAAILQPPYFDPKAD
                     PAVNYGSIGAIIGHEIGHGFDDQGRQFDPTGRIRDWWTAGSSTEFNKRAEKLGKQYDQ
                     YEPIPGTKINGKLTMGENIGDLGGLEMAYAAYKRYTAKHGEPKVLGGLTGDQRFFIAY
                     GQSWRSKVREGAMRERLLTDPHSPPEYRVDGVVRNVDAWYKAFGVKPGDKLYLPPEER
                     VSIW"
BASE COUNT         1832 a         4200 c         4280 g         2089 t
ORIGIN      
        1 tgcgcgaacg tccctcgact gcgctcggga cgaacggctg cgtgcggttg tcacttcacg
       61 tctccttcac cgggctgccc aagctcgaag tcgctgaccg ggcgccgccg cgggtcgttc
      121 gcatcgtaca tcagggcgcc gtcgacccag acccgctcgg gccgggtgta gacgctgaac
      181 ggatgcccgt tccacagcac cacgtcggcc atcttgccgg gcttcaggct gccggtcttg
      241 tcggcgatcc cgagcgcccg cgccgggttg aggctgagcc aggtccaggc gacctcgtcg
      301 gtgatgtcga tcccggcccg gcggccgttg gcgagcgcct tcgcggcttc ctggtagagc
      361 cgctggatgc cgttggcgtc gtccgaatgg acgatcgcgc aggcgccggc gttgtggacg
      421 aaggggatat tctcctggat gccgtcatag gcctccatct tgaaccccca ccagtccgcc
      481 cacatcgccg agcagacgcc gttggccttc aggaggtcgg cgatcttgta gctctcgacg
      541 gcgtggtgga aggtcgaaac cttgtagccg aactccttgg ccatatccat gatgttggcc
      601 atctcgtcgg cgcggtagca atggttctgg acggtgatct cgccggcgag cacgccgcga
      661 agcgtgtcca tggcgaggtc ccggccgggc atctcgccgc cgtcgcgctc atatttgtcc
      721 cacttgcggc gatattcctg cgcgcgcgcc caggtctggc gcatcagcgc gatgttcgcc
      781 atccggctgg cgggcatctg gttgcggccg ccatagacac gcttcgggtt ctcgccgcac
      841 gccatcttga gcgaataggg ggcgtccggg aacttcatct gctgcatcgt tcgcgccggc
      901 acgttcttca acgtgacgcc gcggccgccg aacaggttgg ccgagccggg gaggacgtgg
      961 aggctggtga ccccgccgtt ggccagcgcc cggctgaagc ccggatcctg cggccagacg
     1021 ctgtgctccg cccagacctc ggagcggacc gggccggtca cctcgttgcc gtcctggtgc
     1081 gcctcgaccc cggggctcgg atagtcgccc agatggctgt ggatgtcgat gatgcccggg
     1141 gtcacccact tgccgcgacc gtcgatccgc gtcgcctcgg cggggatcgc cgtgtcgggg
     1201 ccgccgaccg cgcggaccac gccgtcggcg agaacgaccg tgccgttctc gatccggccg
     1261 ccctcgccgt cgaacaccgt cgctccggtg atcgccgtca ccgcgccggg atagcggcgg
     1321 taggtcgacg ggaagggatc gcgcgaatag gtcgcgggac gggccctggg cgtctggtcg
     1381 gaggcgctcg cgggcgggtc cggattggtg catcccgcgg ccgccagggc ggcaaacgcc
     1441 aggctaaggg aaagttgaac ttttcgcgtc ataaggccgt ttgggttggg gttgccacag
     1501 tgtcaagact cgctatattg ctccaggtgg agatcaggat gattaataaa gaactggatt
     1561 agcgcaattt tttcggcggc agcgtgatgt cgcgccacac ttgcggcagg ggatcccgcc
     1621 agaccagggc acgctcgtaa agggctatcg ccatctcgag ataatgggca ttcgagcctt
     1681 ggcggccgcg aatgtggttc agtgtcgagc cgatcacctg aacgatgatg ccgtcataga
     1741 ggttcaacag ccgttcctcg ctgatgtacc cgtcgcgcca gagccatccc atcgtttcga
     1801 tatagccggg cagcagcatc agcacgtcgt agcgggcttt cgcggcctcg ccgcgctggc
     1861 ggccttgggc atccttggcg taggcggacg cggcggcatg tcggagcatg tccagatagc
     1921 gcgagacgat cttgcatcga tagtcgctct tcgacaggct ccgggttcga tggccccagc
     1981 gagcatcgat ggagctgaaa agcgttcgaa gggccacccg tgacgggcgc atctccgggc
     2041 cctcgatcgt ctcatcgatc cgaagcagaa gattggcctc ggcgatttga acgttttcct
     2101 tgtggtgctc cgccacgatt cgctgctggg tagccgccag ctccgcctgc cgcgccgcca
     2161 cctgggcgtg ccgggccgtg gctttcgcct gctgggccgc actgcgacca tattgggcgg
     2221 tcagttcggc ttgctgcttg gaaatctcgg cgtgccgggc ggcggcctcg gcatgtctgg
     2281 cggcggtctg ctgcgccgcc acgccggcct gctggccctc ggccagatgg cgcagctgcc
     2341 tcagtcccag ataggtcgcg gcgatcgcgg tcaaggccac gatccaatcc ggatagcttc
     2401 ccagctcgat cccggcattg ccggcctggt tgttggcgag ggccggcact tcgaacagga
     2461 tcgtagcggg ccatagcgcc cgctgcgaaa agcggcggcg tcctgaatgg ttattccctg
     2521 gcggcgtctc aattaacgcc atgcatccac ttcttcagca ggaaggagaa tacgaacagg
     2581 aaggcgccga tgccgatcgc gaccaggccg atcgtcttga acacgcccat gtaggtcgaa
     2641 aggctcagcg ccgggttggt cacctcgccg ccgaccgtct cgacgctggc gaactgcgcc
     2701 acgactccgc cgacatattg cgccatcgcc gagctgagga accagacgcc catcatcagg
     2761 ccgacgacgc gggcgatgga cagcttggtg atcatgctga ggccgacggg cgacaggcac
     2821 agctcgccga tcgagtggat cagataggcg agcgccagcc agatcagcgg caccttgtag
     2881 tcgccgccgg cgaattgcga gccgaacacc agcaccagga agcccgctcc gacgagggtc
     2941 agtccgatcg cgaacttgac cgggatgctg ggctcgagcc gcttcctcgc cagcgccacc
     3001 cacatcaggc tgaacagggg cgcgagcagg acgatgaaga tcgggttgaa gatctgggtc
     3061 tgtccggccg gcatcagata gccgccgatc tggcggtcgg tgttgcggtc ggcgaacagg
     3121 gtgagcgacg agcccgcctg ctcgaacagg gtccagaaga cggtcgagaa gacggtgagg
     3181 atcaccgcca ccaccatcat gtggaactcc tcgcgcgtgc ccttcagata ggcccagatc
     3241 gggatcccga tgacggcggc gaaataggtc cagaacatca cctggccgag gatcggaagc
     3301 gaggcgatgt agcccaggat cccgccgccg gactgcgccg catgggcgga ggcggccgcg
     3361 ttggccatgg tgttgttgag caggaaccag gcgaccggga tcatcgccag ggcccccagg
     3421 gtgacgagca tcagggtctt gtggctgtgc tcggcgggag gctcgccata gccgttgagc
     3481 cggccgccgt cgaactggaa cagagcccag gcgatcagca ttccgaaggc ggcaaggccg
     3541 aagccggccc accagccgaa ccacaccgcc agcagcgggc agaagaactg gctgatcagc
     3601 gagccgaggt tgatgcccat gtagaagatg gtgaagcccg cgtcccggcg gctgtcggtg
     3661 gcgtcgtaga gcgagccgac gatggtcgag atgttgggct tgaacaggcc gttgccgacg
     3721 atcaccgccg agagcgagaa cagcatcagc atgacgaaga agggatcgcg ctcgccgccg
     3781 gtctcgtact ggcccttggc gacgctgggc ggcagggcgg cggccgcggc cggggctgcc
     3841 ggagcctggc cggcggcggc cggagcggcg cccgccggag tgaggttcac cgaaccgtcc
     3901 tcattgccct tgaacaggta gcgctggccg ccggcgatga catattgggt ggcttcctcg
     3961 ccctggcgct cgatctggac ctcgtagcgc tggccgccgg tctcgaagaa gggcttggcg
     4021 gccgggccgt tgaagcacag gccgagatag cccagcgaca tcaggatcgc gcccagcttc
     4081 accgagcgct tcgagcccag gtagcggtcg gcgacgaggc cgccgatcag cggggtcagg
     4141 tagacgaggc tggtgaaggc tccgtagagc ccgttcgcgg tggaatcgcc gaacaggaaa
     4201 tgctgggtga ggaacagcac cagcagggcg cgcatgccgt aatagccgaa ccgctcccac
     4261 atctcggtgg tgaacagccg ggcgagctgg cggggatggc cgaaccaggt gtcgcggtcg
     4321 cttcggctga cgttgggctt ttcgccttcg gtcgtagtct cgggagctac attgaccatc
     4381 gctcactctc ctggtcgggc atcgctcgtg ccggcggcct cgttcgccgc ggcaccctag
     4441 cggcgaatcc gcccgtgtga agccggattg ttgcgttgtc tcaaggctgg tagcgcccgg
     4501 cgaatgctca acgaccgcac ctcgctcctc tcctacctcg ccacgcgccg gtcggcgcgg
     4561 ccgcgcgacc tggtcgagcc ggggcccgat ccggaccagc ttcgccggat cctcgagatc
     4621 gccgcccgca gcccggacca cggcaagctc gcgccgtggc gcttcgtcca tgtcgcgcgc
     4681 gaccggcgcg ccgccttcgc gcagacgctc gaggccgcct atcgctcggg caaggccgag
     4741 ccgcccagcc ggatcgagat cgaggccgtc caccgcttcg ccacccaggc gcccgagctg
     4801 atcgtgctgc tctcctgccc ggtcgagggg accaagatcc cggtgtggga gcagcttctc
     4861 tcgtgcggag cggtcgggat gaacctcctc cacgccgcct cggccctggg cttcgccgga
     4921 ggctgggtga ccggctgggc ggcctattcg ccggcggtgc tggaggcgct cggcgggcgc
     4981 gagccggagc gggtcgccgg attcttcttc ctgggaacgc cgggcaccga gctggaggag
     5041 cgcgtccggc cggcgctcga cgatgtggtt cggcagtggt gacaccctcg tcatccccct
     5101 ccccgagcag gctcggggag gatttcgcgc gcttgactgc cacactgtat tagctcacta
     5161 tagcggcgtg tccgaagaac gccccgtcta tctccgcctg cgcgacaata tcggcgcgat
     5221 gatcctcgaa ggccgagtcg ccgacggcga tccgctgccg tcggtgcgaa gcctcgccgc
     5281 cgagttcggc gccaacccgc tcaccgtcgc caaggcctat cagagcttcc aggacgaggg
     5341 gctggtgctg gtcaggcgcg gcgtcggcat gttcgtcgcg cccggcgcga ccgaccggct
     5401 gcgcaaggcg gggcgcgagg acttcctcaa ccaccgctgg cccgagatcg tcgcccagat
     5461 ccgccggctc ggcctcagcg ccgaggagct gctcgagcgg gtctcggcct gatccttcga
     5521 tacgggcctt cgccaggctc agtccctact caggatgagc ggatggagag tggttgccac
     5581 cgctcatcct gagtagccgt tgagtttggc gaaacggcgt atcgaaggac tgacacaatt
     5641 gacaccaact tcccggttcg acggcatcgc tccgacacgc tcggcggcga aatctggcct
     5701 cgcggcttcg gccggcggcg tatcgccccc tccgcctccc ggccggagcc gctccccgcc
     5761 cggggatcac gccaacgagg acccgaccgc catgcacccc gcccgcgcct tcttcacctc
     5821 gctctcgatc agcctcgtcg tgctgacgct gagctaccag gcagcggcgc tcccggcgat
     5881 catcgcgccg cccaagccgg tgccgatgcc ggccagcaac gcgtggatgg acaatgccga
     5941 ggcggtgaag gccaagggcc gcagccaaga cgacatgtcg gagctcgcgc cgctggccga
     6001 cgttctctga gcctaatttc ctccccgcga tttcgcgggg aggggaccgc gcgaagcgcg
     6061 gtggaggggt tcttcagact cggcaagaac ccctccacca ggcttcgccg ggccccctcc
     6121 ccaggaaatc ctggggagga ttgagacgcc tagccccgct tggcccccgc cgtccgctcc
     6181 ctgatccgcc gctccaggat ggtgagcggc atcgcgccgt ccttcagtat ctcgtggaac
     6241 cgcttgaggt cgaagcgccg cccgagcgcg cgctgcgcct cggtccgggc ccgggtccag
     6301 gccatgtggc cgaccttgta gctgcacgcc tgcccggcct gcgtgcagta gcgctcgacc
     6361 tcgcgctggg agcgggggcg ggcgaagccg gtggtggcga cgagatagtc ggtcgcccgc
     6421 tcgcggctcc actgtttggc gtggaggccg gtgtccacga ccagccgggc ggcccggaac
     6481 aggaaggact ggagatagcc cgcccgctcg agcggagtcg cataggcgcc gagctcgtcg
     6541 gcgagctgct cggcataaag cgcccagccc tcgctatagg cgctgaagcc gccgaccttg
     6601 cggatcgtcg ggatgtcggc cgattcctgg gccaggctga tctggaggtg atggccgggc
     6661 gagccttcgt gataggtcag ggccggcagg ctgtacttcg gccagtcgcc cgtatccttg
     6721 aggttgatga agtagatggc cggccgcgag ccgtcgagcg aggcgcggtt gtaatagccg
     6781 ttcgaggccc cgtcctggat ctccaccggc acccggcgga tctcgagcgg cgccttgggc
     6841 agggtggcga aggcctgggg cagccgcgcg tccatcgcct tcacgccctc gttgaggccg
     6901 gcgatcagct cggcccggcc ttcgtccgta ttgggataga gctgggaggg actgacgttg
     6961 agcgccgcca gccgctcgcc gacgctgccg cggctcatgc cctggctcct gaggatccga
     7021 tcgagctggg cgctgatttc ggcgacctgc tgcaggccca tgcgatggac ctcgtcgggg
     7081 gtgaagcgcg tcgtcgtcgc ctgctcgagc gcgaccgcgt agatctcgtc gccgtgcggc
     7141 acccgcccga tgccgtcgcc gggccggctg gccgcgcgga gatcgcggac cagcgccatc
     7201 tggcggtcga gggcgggata gacgcgctcc tcgacgatcc gctcggcccg cctctcccac
     7261 cggccctcga tgtccttggc cttggcgcgc ttgaccagcg actggaccat gctgctctcg
     7321 tccgccttct gctccctgag cttggccatc tggcccagcg tcaggtcgag cgaccaggcc
     7381 ggggcgagat agccgcgcga cgcctcgatc cgctgcatcg cgctgtcctg gtcgagcgcg
     7441 accgcgaacg cctcgagccg cgccagatag gcctcggcgt cgtcccgggt gttgatcgta
     7501 tgggcggtgt tgaggaagtc gggcaccgag aaataggcgc cgccctgctg gaagatgcga
     7561 tagggtcgga tcgcgctgtc cagcccgaag cgctgcggcg cgatcgtctg gttggtcagc
     7621 gagtaggtga cgacctcgag gttgagcctg gccgccggcg acaaggccga ccggtcgacc
     7681 gcggcgagct ccgccagcgc cgccttgacc tgcgccagct gccgtgcccg ctcggcgagc
     7741 gttcgggggg tgagccggcc cttgagcccg gcatattcgc ccttgtcgag gccgagctgg
     7801 gtggcgagct ccggcgagcg ctgcaccgcc tgctggaaga tgcgctcgaa cagagcgttg
     7861 agctcggcgt cgcccgaacc ggcgcgcggc tgggcgagga cggtcgcggg aagcgtggcg
     7921 gcgagggcgg cggcgcccga cgaggcgatg aaggtgcggc gatccaaggg gaacctcctt
     7981 tgagtcgagc tgcgcctcta ggccggaggc gcggccggcg caaagccgtc gttgcgagga
     8041 gcgcagcgac gaagcaatcg aggtacagcg cctgcgaccg aggcggcact ggattgcctc
     8101 gcttcgcccg caatgacagg ggcgtaacaa tcgacacaat tttccctccg ccgcggcatg
     8161 ctgcggtcac ccgccccctt cattgagcgc accacaggct tccggccggc ggcgtacccg
     8221 ccccctccgc gccggccgga gccgacccca caacaatgga ggaccctgtg ttcggtctct
     8281 accatcagat cgcgctcgcg ctttttgcgg gcttcgttct cgccgagctc ctgctccccg
     8341 gccgcgcctt cccgaccctg cgcctctggc gcctgcgggc ggcgctttcg accatcgcct
     8401 atttcgccgt cgcgaccttc gcgccgttga tctgggacgg cacgctcggc gcgtaccggc
     8461 tgttcgacgc caccgccctg cccttctggg cgcaggtggc cggcggcttc ctggcgctcg
     8521 agctgggagt ctacgtctgg caccgcacga tgcacaacgt ggacccgatc tggcgctggt
     8581 tccaccagat gcaccacagc gccgagcgca tcgacgtctg gggcgccttc tacttctcgc
     8641 cgctcgacat gctgggcttc accttcctgg gcagcctcgt gctggtgctc ggcttcggcc
     8701 tcggcggcga ggcggcgctg gtcgtcaacg tcttcgtcac cttcctcgcc atgttccagc
     8761 acgccaacat ccgcacgccc cactggctcg gctatctgat catccggccg gaaagccatg
     8821 cggcgcatca tgagcgcgga gtgcacgcct tcaactattg cgacctgccg ctctgggaca
     8881 tgctgttcgg cacgttcagg aacccgcgcg agttcgaggg cgaggcgggc ttctgggacg
     8941 gcgcctcggg ccggatcggg ccgatgctgc tggggaggga cgtggcatga tccgcttcct
     9001 cctcgccgtc ctcgccgcct acatgctcgt gctgggagcg gccgcttccg ccgcgccgat
     9061 cgaccagggc ggctggaacg cgcgcaagca gagcgtccgc ctcgccaacg gcgtccgcct
     9121 ggcctatgtc gagctcggcg acccggacgg cgcgccgctc ctcctgctcc acggctatac
     9181 cgacacgagc cggagctgga cccaggtcgc gccctggctc ctgcggcacc gcctgctcgt
     9241 ccccgaccag cgcggccatg gcgggtcgga tgcgcccgcc tgctgctatt cggcctcggt
     9301 tttcgccgag gatgcgcgcc tgttcctcga tgcgatgaaa gtggagaggg ccgccgtcgc
     9361 cggccattcg ctcggctcga tggtggcgat gacgctcgcc gccgagcatc ccgagcgggt
     9421 gacgagggtg gctctgatcg gctcgaccgc gctggttccg gtgaagcgcg gcgactggct
     9481 gtggaccaac gcccatgccc tgaaggcgcc gctcgacccg agctccgact tccttcgcga
     9541 atggcatccc gccaatcagc cgactccggt cgacaaggcc ttcgccgagg ccgccatggc
     9601 cgagatcctc gccatcccgc tccacgtctg gcgcggagtc atgcgcgagc tcgccgaggt
     9661 ccccgccggc cgccacgccg ccgacgtcgg ggcgccggtg ctgatcctct ccggcggcaa
     9721 ggacccgctg ttctcggcgg agcatcatgc ggcgctggtc aaggccttcc ccaaggcgga
     9781 gtcgcaggtc ttccccgatc tcggccataa cctgaactgg gagcggccgg ccgacgtggg
     9841 cccggtgctg gcgcggtttt tcgcagccga gcgctgagct gaaacggccg tcattgcgag
     9901 cgaagcgaag caatccagtg cggccttcga ggtccgctgg attgcttcgt cgctacgctc
     9961 ctcgcaatga cgtcgccgca cctaccagat gctgacgcgc tcttccggcg gcaggtacag
    10021 cttgtcgccc ggcttgacgc cgaacgcctt gtaccaggcg tcgacgttcc tcacgactcc
    10081 gtccacccga tattcgggcg ggctgtgcgg atcggtcagc agccgctcgc gcatcgcccc
    10141 ctcgcgcacc ttcgagcgcc agctctggcc gtaggcgatg aagaagcgct ggtcgccggt
    10201 gaggccgccg agcaccttgg gctcgccatg cttcgccgtg tagcgcttgt aggcggcata
    10261 ggccatttcg agcccgccca gatcgccgat attctcgccc atggtgagct tgccgttgat
    10321 cttcgtgccc gggatcggct cgtactggtc gtactgcttg ccgagcttct cggcgcgctt
    10381 gttgaactcg gtgctcgagc ccgccgtcca ccagtcccgg atccggccgg tcggatcgaa
    10441 ctggcggccc tggtcgtcga agccgtggcc gatctcgtgg ccgatgatcg ctccgatcga
    10501 gccgtaattg accgccgggt cggccttcgg gtcgaaatag ggcggctgaa ggatcgcggc
    10561 cgggaaggtg atctggttca tcagcgggtt gtaataggcg ttcacggtct gcggggtcat
    10621 gtcccacagc gcccggtcca ccggcttgcc gagccgcgac agctgcagct cgtgggcgaa
    10681 cttctccgac ctgacgacat tgccgagcag gtcgccgcgc ttcacgtcca ggctcgaata
    10741 gtcgatccac ttgctcggat ggccgatcct cgggtcgaag gcggcgagct tggcccgggc
    10801 ctgcgccttg gtcgcgtcgt ccatccaggt cgaggcggcg aggcgcgcct cgagcccgga
    10861 gcgcagattg gcgatcagct cggccatctg gcggttgctc tcgggcggat aatgacgctc
    10921 gacatagacc cggccgacgg cctcgccgag cgagccgttg atgatctgga ctccgcgctt
    10981 ccagcggtcg cgcatctgcg gcacgccgcg cagggtcttc gagtagaagt cgaaattggc
    11041 ctggtcgaac gccttgggca gatagggggc gtggctgctg acgaagtgga aggcgagata
    11101 gtccttccag gtcgacagcg gcacggagtc gagcatcttg ccggtctcgg tgatcgcgct
    11161 cttctccgcg gcgatgacgg tctcgacgct gcccaggccc gcggcgtcga ggaaggtcgg
    11221 ccattcgagc tgcggcgcga gctccgcgag ctgggcgcgg ttcatcggat tgtagatctg
    11281 cttgatgtcg cgctggcgcg ccggctccca atggaccttg gcgagcgccg tctcgagcgc
    11341 gatgatccgg tcggccttgg cttcggcgtc ggcgatcccg gcgagctgct ggatcttgac
    11401 gacataggcg cgataggcgg cgcggatcgc gtcgtacttc tcgccctgga gcagataata
    11461 gtcgcggttg ggcaggccga gcccgccctg gccggcggcg gcgatgtagc gggtcggatc
    11521 ggccgggtcg ggcaggatgc ccactccgac cgggctgatg actccgggct gcgcgaaggc
    11581 gcggatcagg tcggtgcggt tggagatggc ggcgaacctg gccagatagg gcttgagcgg
    11641 cgcggtcccg gccgcctcga tcgccgcctc gtccatccag ctggcgtaga agtcgccgac
    11701 ctgctggccg atcttgccgg accgcgccgg gtcgcgcgcg aggtcctcga cgatcgcgcg
    11761 gacctggcgc tcggcctcgt cgaccagcag gactccggcg ccggcgctgg tgcggtcggc
    11821 ggcgatctcg gtggtcttgt cccaattgcc gttgacgtag tgccagaagc tgtcgcccgg
    11881 cttgaccgag cggtccatcg cggaaaggtc gatgccgaaa tcgccgtagc gcggcttggc
    11941 cgaggcggcc gccggcgccg ccgctccggg ctgggcgagg agcggcgttg cggcgccgcc
    12001 gagcagaagg gccgccgtaa aggcgagacg aaagttgcgc atgtgaagat ctccccgggc
    12061 cggattcggc cgtcgtcccg acggttacca acccgcgccg cgccccgcaa cgcgcgcaat
    12121 tgtaaatgag caaagtcggt cgggaccgtc ccgaattcct ccccgcgatt tcgcggggag
    12181 ggggaccagg cgcagcctgg tggaggggtt cttcggaccc aggaagaacc cctccaccgc
    12241 gcttcgcgcg gccccccttc ccggcaaaag ccggggagga atttccatcg cccctcctcc
    12301 cgccccgccg ccgccgcgat tcggcgctcc gtggtggact cgggagcggc gcggcggtta
    12361 aaggcgccgc cgggaagggt tttcgtgttc atgccgaagt t
//
LOCUS       DASVQZ010000088         3633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_498021, whole genome shotgun sequence.
ACCESSION   DASVQZ010000088 DASVQZ010000000
VERSION     DASVQZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498021"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VF547_02475"
     CDS             <1..1419
                     /locus_tag="VF547_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX8261719.1"
                     /translation="VPLGTAIGLKVRERLTGEAPPAAARGGIDLVSEWIEDKAGADLD
                     ALGLVLDDQAAFAALATKLLRDLELIEGEAEAESDPDEGDEGEGGDKDQGDEGEAQDE
                     EDEGGASGESEMRAEPGDSSDEEGERDWSEEEMAEGEEGLGEEGEEGMLPVRPNRPLS
                     DLPPQFDYRIFTTRFDETIEAPELCDEEELGRLRAYLDQQLVHLQSAVTKLANRLQRR
                     LMAQQNRSWDFDQEEGLLDAARLARVIVNPAHSLSYKVERETEFRDTVVSLLLDNSGS
                     MRGRPISIAAISADILARTLERCGVKVEILGFTTRAWKGGQARETWLAQGRPPNPGRL
                     NDLRHIVYKAADEPWRRARKNLGLMMREGLLKENIDGEALLWAHNRLIARPEERRILM
                     VISDGAPVDDSTLSVNSGTYLERHLRQVIAWIQSRSPVELVAIGIGHDVTRYYERAVT
                     IMDAEQLGGTMIEQLAALFDAP"
     gene            complement(1521..1880)
                     /locus_tag="VF547_02480"
     CDS             complement(1521..1880)
                     /locus_tag="VF547_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8261720.1"
                     /translation="MFRKITLATAAAATLAMVPAAAEARHRDGYYGRDGYSQTYRGDR
                     YGRYDSRYYGRTDYRYSGRRCSGTTGTIVGGVAGALLGREVTRDRYRGRSGTTGAILG
                     GAIGALAGRAVQKSGCR"
     gene            complement(2004..2732)
                     /locus_tag="VF547_02485"
     CDS             complement(2004..2732)
                     /locus_tag="VF547_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415171.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8261721.1"
                     /translation="MRSLFPIVAMSLALTACSSSAPDPRGVGRDEVLVQVSASGRADS
                     RPDEARFTVGVETIAPAARAASAQNSATFNRVAAALARLGVREDDLQTRSITLSRIDY
                     GPNRGRFQANNLIDVKVRDIKRAGEAIAAITEAGANVLSGPNLRVSDPENAARSAYAA
                     AYRAARARAEAYAQAAGLKVARVLAIRDGSGGQSPYLRYETGDSVAMEQAAPPPIAVN
                     AAPPVQPGISTREVQIRVDFALAK"
     gene            2829..3470
                     /locus_tag="VF547_02490"
     CDS             2829..3470
                     /locus_tag="VF547_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEX8261722.1"
                     /translation="MQQLIRIAALALAALLATFATPAAAQEQPEGQSKADYLAWLSRD
                     AGARTMVLSFKSFLEAADVDGVVPTWQLVRTASKWRDCGGPRFEVAPASEWVHIADTL
                     RFVKDEVEPVIGDVHPVSGFRNAELNQCSGGAKASAHRHFYALDLVPADSGLARAALI
                     RDVCRIHDSAGREFGIGLGFYTGKRFHLDSKGFRRWGANGSGATSPCATGNYA"
BASE COUNT          561 a         1244 c         1307 g          521 t
ORIGIN      
        1 gtcccgctcg gcacggcgat cgggctcaag gtccgcgagc gcctcaccgg cgaggcgccg
       61 ccggccgccg cccggggcgg gatcgacctc gtctccgagt ggatcgagga caaggccggc
      121 gccgacctgg acgcgctcgg cctggtcctc gacgaccagg cggccttcgc tgcgctcgcc
      181 accaagctgc tgcgcgacct cgagctgatc gagggcgagg cggaggccga gagcgatccc
      241 gacgagggcg acgagggcga gggcggcgac aaggatcagg gggacgaggg cgaggcgcag
      301 gacgaggagg acgaaggcgg cgcctcgggc gaatccgaga tgcgggccga gcccggcgat
      361 tcgtccgacg aggagggcga gcgcgactgg tccgaggagg agatggccga gggtgaggaa
      421 ggcctgggcg aggagggcga ggagggcatg cttccggtcc ggcccaaccg tccgctgtcc
      481 gatctgcccc cgcaattcga ctatcgaatc ttcaccaccc ggttcgacga gacgatcgag
      541 gcgccggagc tgtgcgacga ggaggagctc ggccggctgc gcgcctatct cgaccagcag
      601 ctcgtccacc tgcagagcgc cgtcaccaag ctcgccaacc gcctccagcg ccggctgatg
      661 gcgcagcaga accggagctg ggacttcgac caggaggagg gcctgctcga cgcggcgcgg
      721 ctggcgcggg tgatcgtcaa cccggcccat tcgctcagct acaaggtgga gcgcgagacc
      781 gagttccgcg acaccgtcgt ctcgctgctg ctcgacaatt cgggctcgat gcgggggcgg
      841 ccgatctcga tcgccgcgat ctcggccgac atcctcgccc gaaccctcga gcgttgcggc
      901 gtcaaggtcg agatcctcgg cttcaccacc cgggcgtgga agggcgggca ggcgcgggag
      961 acctggctgg cgcagggccg accgcccaat cccggccggc tcaacgacct gaggcacatc
     1021 gtctacaagg ccgccgacga gccttggcgg cgggcgcgca agaatctcgg cctgatgatg
     1081 cgcgagggcc tgctcaagga gaatatcgac ggcgaggcct tgctgtgggc gcacaaccgg
     1141 ctgatcgcgc ggcctgagga gcggcgcatc ctgatggtca tctcggacgg cgccccggtc
     1201 gacgattcga cgctgtcggt gaacagcggc acctatctgg agcgccacct gaggcaggtg
     1261 atcgcctgga tccagtcgcg ctcgccggtc gagctggtgg cgatcgggat cggccacgac
     1321 gtcacccgct attacgagcg ggcggtgacc atcatggacg cggagcagct tggcggaacg
     1381 atgatcgagc aattggcggc gctcttcgac gcgccttgat ttgcctcccc acgcgaacgt
     1441 ggggaggcaa aaaagaaggg ccggcctccc tcaggagacc ggccccgccc tcgatacagt
     1501 accggagggt tacgccaagc ttagcggcag cccgacttct ggacggcccg cccggcgagc
     1561 gcgccgatcg ctccgccgag gatcgcgccg gtggtgccgc tgcggccgcg ataacgatcg
     1621 cgggtgacct cgcggccgag cagggcgccg gcgactccgc cgacgatggt gccggtggtg
     1681 cccgagcagc ggcggcccga atagcgatag tcggtccggc cgtaatagcg gctgtcgtag
     1741 cggccgtagc ggtcgccgcg ataggtctgc gaatagccgt cgcggccgta atagccgtcg
     1801 cggtggcggg cctcggcggc ggcgggaacc atggccaggg tcgcggcggc ggcggtggcg
     1861 agagtgattt tcctgaacat tcgaaactcc ttggggcggc ggacacatcc cggccgctgc
     1921 acgaatcgct tctgcctccg cagccttgag ccgctcctga atgatgccgt cagccgccgt
     1981 tcagcctcgc ggcccgccgc ttctcatttc gccaatgcga aatcgacccg gatctggacc
     2041 tcgcgcgtgg agatgccggg ctggaccgga ggcgcggcat tgaccgcgat cgggggcgga
     2101 gcggcctgct ccatcgccac cgagtcgccg gtttcgtagc gcaggtacgg cgattggccg
     2161 ccggagccgt cccggatcgc caggacccga gcgaccttca gcccggccgc ctgcgcatag
     2221 gcctcggccc gcgcccgcgc cgcgcgatag gcggcggcgt aggcggagcg ggcggcgttc
     2281 tcggggtcgg agacgcgcag gttcgggccc gagaggacgt tggcgcccgc ctcggtgatt
     2341 gcggcgatcg cttcgccggc ccgcttgatg tcgcgcacct tgacgtcgat caggttgttg
     2401 gcctggaagc ggccgcggtt ggggccgtag tcgatcctgg acagggtgat cgagcgggtc
     2461 tggaggtcgt cctccctgac ccccagccgc gcgagggcgg cggcgacgcg attgaaggtg
     2521 gcgctgttct gggcgctcgc ggccctggcg gccggggcga tcgtctcgac cccgacggtg
     2581 aagcgggcct cgtcgggccg gctgtcggcc cgtccggagg cggagacctg gacgaggacc
     2641 tcgtcccggc cgacgccccg cgggtcgggc gccgagctcg agcaggccgt cagagcgagg
     2701 gacatggcca cgatcgggaa aaggctgcgc atcggtcgga ctccaatggt ttagcgcgtt
     2761 ataacattac attcccgcgc gccgccagac cccttgaaaa atggcgagaa tgtggctatc
     2821 tatctgttat gcaacaactt atccgcatcg cagcgctcgc gctggccgcc cttcttgcaa
     2881 ccttcgccac cccggccgct gcccaggagc agcccgaagg ccagagcaag gccgactatc
     2941 tggcctggct ctcgcgcgac gccggcgccc ggacgatggt gctctccttc aagagcttcc
     3001 tcgaggccgc ggacgtcgat ggcgtcgtcc cgacctggca gctggtccgc accgccagca
     3061 agtggcgcga ttgcggcggc ccgcgcttcg aggtcgcgcc ggccagcgaa tgggtgcaca
     3121 tcgccgacac cctgcgcttc gtcaaagacg aggtcgagcc ggtgatcggc gacgtccatc
     3181 ccgtctccgg cttccgcaac gccgagctga accaatgctc cggcggcgcc aaggcgagcg
     3241 cccaccgcca tttctacgcg ctcgacctcg tcccggccga ctccggcctc gcccgagccg
     3301 ccctgatccg cgacgtctgc cggatccacg attcggccgg ccgcgagttc gggatcggac
     3361 tcggcttcta caccggcaag cgcttccacc tcgattccaa gggcttccgc cgctggggcg
     3421 cgaacggcag cggcgccacc agcccctgcg ccacgggcaa ttacgcctga ccgcggcccg
     3481 ctacgtgatg tcccgaggat ggaaggttcg ctcggccgcg cgataagggc cgccgcaccg
     3541 gccccgggag agttcgcgat ggacgtgcag gaagcgagga cgttcaggag cggccgcggc
     3601 gtcgctcttc ggctcccgga cagcctcgcc atc
//
LOCUS       DASVQZ010000089         4359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_117261, whole genome shotgun sequence.
ACCESSION   DASVQZ010000089 DASVQZ010000000
VERSION     DASVQZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117261"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF547_02495"
     CDS             <1..560
                     /locus_tag="VF547_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261723.1"
                     /translation="GTVRLRRGVLPAALAAATVALVFAFAWFMQDPGRWPFEALESSF
                     RETAAGYAKGEVVSGRPETFHAPPARLLDFVLISADRFLHFFAVDAARFSDIHRAAQM
                     VFFLPCFALAAWLAIALWRGRTPFGPAERKVFLAAFGALFSYALFHALVQVDFDWRYR
                     LPVVPHLILLAAGGLGDLARREAAR"
     gene            557..1399
                     /locus_tag="VF547_02500"
     CDS             557..1399
                     /locus_tag="VF547_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8261724.1"
                     /translation="MTGEALYLFSVDVEDPRTGVAQGDALPPRVPAMVDLYLEWLARS
                     SAKGTFFVVGEVARRHPEVVRRIAEAGHELGCHSDSHLPLDRLGRAAFRDDLERNLDA
                     LRSAGAGEIKGYRAPCFSATAGTGWMYEALAGLGFDYSSSVLPARNPQYGQPGFGVRP
                     RLIEGIVELPVTLLPWRWLPVPFGGLYFRVLPRPLLRRALAACKARGEAVASYHHPYD
                     ADTEQNFTHAGFRRWGPFDLLMRSNRDAVMPRLAMARAMGFEFAPYGPFAAAARADLS
                     RLAA"
     gene            1396..2247
                     /locus_tag="VF547_02505"
     CDS             1396..2247
                     /locus_tag="VF547_02505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8261725.1"
                     /translation="MSAEEAYRLAPLAMSGDGFERVSALLRRVFPRARHLTPGYLAWQ
                     YSRNPDGEAVGFSAYAGDALVGHLAGVALSARIEGEIRRGLLLVNSAVHADHRRRSLM
                     SRLSEAIFAEGAVRGYSFSISTGNRYSSKPLLTRYQPIGPLEARIGIGRSRRAAMPAP
                     ASFERIWSEEALRWRLANPERRYCVRRRGGQLSISAATGLPGVAALLYQGAGEAPPTA
                     GSAPGPLGLWLGRDPGIAWRRSSFLPIPERLRPSPLNLFFRDLTGGGFVPDPDRLIFQ
                     ALDFDAY"
     gene            2279..2647
                     /locus_tag="VF547_02510"
     CDS             2279..2647
                     /locus_tag="VF547_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261726.1"
                     /translation="MTDTRPDPAEAEPPLSLQEAVDAGIRRISTALVVAGAIIGLAVY
                     ARPGPPRFEAVVDGDRIIRVDTHKGTIISCEGQQTCQLILKSGQRLTRVKRTDAPPQA
                     EALPKPAVPTPPAAAPAAEK"
     gene            complement(2610..4172)
                     /gene="metG"
                     /locus_tag="VF547_02515"
     CDS             complement(2610..4172)
                     /gene="metG"
                     /locus_tag="VF547_02515"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX8261727.1"
                     /translation="MAEPFYVTTAINYPNGPPHIGHAYEAVATDVIARFQRSMGRDVL
                     FQTGTDEHGLKMAQAARERGISPARLADEMSHIFKRMDDRLEISYDRFIRTTEPAHHR
                     ASQAIWRAMADRGDLYLGRYEGWYSVRDEAYYDEGELIDGADGGRLSPQGTPVEWTVE
                     ESWFFRLSAYQEQLLAHYRNNPGFIRPESRRNEVLRFVEGGLSDLSVSRTSFDWGVKV
                     PSDERHVMYVWLDALTNYLTGIGYPDTHSETYRRFWPADLHVIGKDVVRFHAVYWPAF
                     LMSAGLPLPRQVFGHGHVLLRGEKMSKSAGNVVDPVGLIDSFGADALRYFLMRDVTFG
                     QDGSYSPEAIVTRVNADLANSFGNLAQRTLSFVAKNCGGELPSGGRGEPADAELLELV
                     RRTTAADVPALFGELSLSQGIEAWIRAVFACNQYIDVQAPWTLRKTDPERMTAVLATL
                     CEAIVDLAIAIAPVVPGSAAKLLDQMGVPDAERSFGALPDTERYARLAQSKVLLEPPS
                     PIFPRLELPPEG"
BASE COUNT          595 a         1556 c         1511 g          697 t
ORIGIN      
        1 ccggcacggt ccggctccgc cgcggagtcc tgccggcggc gctggcggcc gcgaccgtcg
       61 ctctcgtctt cgcctttgcc tggttcatgc aggatcccgg gcgctggccg ttcgaggcgc
      121 tcgaatcctc cttccgggag acggccgcgg gctatgcgaa gggcgaagtg gtgagcggca
      181 ggcccgagac gttccacgcg cccccggcaa ggctcctcga cttcgtgctg atctccgcgg
      241 accgcttcct tcacttcttc gccgtcgacg cggcgcggtt cagcgacatc catcgggcgg
      301 cgcagatggt cttcttcctg ccctgcttcg cgctcgcggc ctggctcgcg atcgcgctgt
      361 ggcgcggccg cactcccttc ggcccggccg agcggaaggt cttcctggcc gctttcggcg
      421 ccctgttctc ctatgcgctg ttccacgctc tggtgcaggt ggatttcgac tggcgctatc
      481 gcctgcccgt cgttccccac ctgatcctgc tcgccgccgg cggcctgggc gacctcgccc
      541 ggcgcgaggc ggcgcgatga cgggtgaggc gctctatctg ttcagcgtcg acgtcgagga
      601 cccgcgcacc ggagtcgcgc aaggcgacgc cctgcccccg cgggtgccgg cgatggtcga
      661 cctctatctg gaatggctgg cccggagctc ggccaagggc accttcttcg tcgtcggcga
      721 ggtcgcgcgc cggcatccgg aggtggttcg gcggatcgcg gaggcggggc acgagctggg
      781 ctgccattcg gattcccacc tgccgctgga ccggctcggc cgagcggcgt tccgggacga
      841 cctcgagcgc aatctcgacg ccctgcgctc cgccggagcg ggggagatca agggctatcg
      901 cgcgccctgc ttctcggcga ccgccgggac cggctggatg tacgaggcgc tggccgggct
      961 cggtttcgat tattcgagct cggtgctgcc ggcgcgcaac ccgcaatacg gccagcccgg
     1021 cttcggcgtt cgaccgcgcc tgatcgaggg gatcgtcgag cttcccgtta ccctgctgcc
     1081 ctggcgctgg ctgccggtgc ccttcggcgg cctctatttc cgggtgcttc cgcggccttt
     1141 gctgcgccgg gcgctcgccg cctgcaaggc gcgcggcgag gccgtggcca gctaccacca
     1201 cccctatgac gccgacaccg agcagaattt cacccatgcc ggcttccgcc gctggggccc
     1261 gttcgacctg ctgatgcgct cgaaccggga cgcggtcatg cccaggctgg ccatggcgcg
     1321 ggccatgggc ttcgagttcg ccccctacgg ccccttcgcg gccgcggcgc gcgcggacct
     1381 gtcgaggctg gcggcatgag cgcggaggag gcctaccgcc tcgccccgct cgccatgagc
     1441 ggcgacggtt tcgagcgcgt ctcggcgctg ctgcgacggg tcttcccgcg cgcgcggcac
     1501 ctcacgcccg gctaccttgc ctggcaatat tcgcgcaatc ccgacggcga ggcggtcggc
     1561 ttcagcgcct atgcgggcga cgccctcgtc ggccaccttg cgggagtggc tcttagcgcc
     1621 cggatcgagg gggagatccg gcgcggactg ctgctggtga acagcgccgt ccatgccgac
     1681 caccggcgcc gcagcctgat gagccggctc agcgaggcga tcttcgcgga gggcgcggtt
     1741 cgaggctaca gcttctcgat ctcgaccggc aaccgctaca gcagcaagcc gctgctcacc
     1801 cgctaccagc cgatcggccc gctcgaggcg aggatcggga tcggccggtc gcggcgggcc
     1861 gcgatgccgg cgccggccag cttcgagcgg atctggagcg aggaggcctt gcgctggcgg
     1921 ctcgccaatc cggaacggcg ctattgcgtc cgccggcgag gcggccaact ctcgatcagc
     1981 gcggccaccg gtctgcccgg cgtcgccgcc ctgctctacc agggggccgg cgaggcgccg
     2041 cccaccgcgg gaagcgcgcc gggcccgctc gggctgtggc tcggccgcga ccccggcatc
     2101 gcctggcgcc gctcgtcctt tctgcccatc ccggagcggc ttcgtccctc tccgctcaac
     2161 ctgttcttcc gcgacctgac cggcggcgga tttgttccgg accccgaccg gctgatcttc
     2221 caggcgctcg acttcgacgc ctactgagcg catgaaatcg gccgatggag gccctgccat
     2281 gaccgacacg cgtcccgacc cggccgaagc cgagccgccc ttgtcgctgc aggaggcggt
     2341 cgacgcgggg atccgcagga tctcgaccgc gctcgtcgtc gccggcgcga tcatcgggct
     2401 cgccgtctac gcccgccccg gcccgccgcg cttcgaggcc gtggtcgatg gcgaccggat
     2461 catccgggtc gacacgcaca agggcacgat catctcctgc gaggggcagc agacctgcca
     2521 gctgatcctg aagagcggac agcggctgac ccgcgtgaag cggaccgatg ccccgcccca
     2581 ggccgaggcc ctgccgaagc cggccgttcc tacccctccg gcggcagctc cagccgcgga
     2641 aaaataggcg aaggcggctc gagcaggacc ttggactggg cgagcctcgc gtagcgctcg
     2701 gtgtcgggga gcgcgccgaa gctccgctcc gcgtccggaa cgcccatctg gtcgagcagc
     2761 ttcgccgccg atccggggac cacgggcgcg atcgcaatgg cgagatcgac gatcgcctcg
     2821 cacaaggtgg cgagcacggc cgtcatccgt tcgggatcgg tcttgcgcag agtccagggc
     2881 gcctggacgt cgatatattg attgcaggcg aagacggcgc ggatccaggc ctcgatcccc
     2941 tggctgagcg agagctcgcc gaacagggcg gggacgtccg cggccgtcgt ccgccgcacc
     3001 agctcgagca gctcggcgtc ggccggctct ccccttcctc ccgagggcaa ctcgccgccg
     3061 caattcttcg cgacgaacga cagggtgcgc tgggcgaggt tgccgaagct gttggcgaga
     3121 tccgcattga cccgggtgac gatcgcctcg ggactgtagc tgccgtcctg gccgaaggtg
     3181 acgtcgcgca tcaggaaata gcgcagggcg tcggctccga aggagtcgat cagcccgacc
     3241 gggtcgacga cgttgccggc cgatttcgac atcttctccc cgcgcagcag gacatggccg
     3301 tggccgaaca cctgcctcgg cagcggcaac ccggccgaca tcaggaaggc gggccaatag
     3361 acggcgtgga agcgcaccac gtccttgccg atcacatgga gatcggccgg ccagaatctc
     3421 ctgtaggtct ccgaatgggt gtccggatag cctatgccgg tcagatagtt ggtgagcgcg
     3481 tcgagccaga cgtacatgac gtgccgctcg tcgctcggca ccttcacccc ccagtcgaag
     3541 ctggtcctcg acaccgagag gtccgagagg ccgccctcga cgaaacggag cacctcgttg
     3601 cggcgggatt cgggacggat gaagccggga ttgttgcggt aatgggcgag cagctgctcc
     3661 tgataggccg agagccggaa gaaccagctc tcctccaccg tccactcgac cggggtcccc
     3721 tgcggcgaca gccggccgcc gtcggcgccg tcgatcagct cgccttcgtc gtaataggct
     3781 tcgtcgcgga ccgagtacca gccttcgtag cgccccagat agaggtcgcc gcgatccgcc
     3841 atcgcccgcc agatcgcctg gctggcgcgg tggtgggccg gctcggtggt gcggatgaag
     3901 cgatcgtatg aaatctcaag acggtcgtcc atcctcttga agatgtgcga catctcgtcg
     3961 gcaagacggg ctggcgaaat acccctttcc cgcgccgcct gggccatctt gaggccgtgc
     4021 tcgtcggttc cggtctggaa gaggacgtcg cggcccatcg agcgctggaa gcgggcgatg
     4081 acgtcggtgg cgaccgcctc gtaggcgtgg ccgatatgcg gcggcccgtt cggatagttg
     4141 atcgcggtgg tgacgtagaa gggctcggcc atacccgccc ttagcggctc ggagcgagcg
     4201 atgcgagcat gccggcgagc tcgaacacca cgctttccgg ctccagcgac aggccacggg
     4261 cgctcgcggc gagggcctgg gctcgctccc aggcgccgag cgcctcggcc agggccgggc
     4321 cgccgcgcgt tctcgcttcg gcggcgatga ccgacggcg
//
LOCUS       DASVQZ010000090         4832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_885742, whole genome shotgun sequence.
ACCESSION   DASVQZ010000090 DASVQZ010000000
VERSION     DASVQZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885742"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..353
                     /locus_tag="VF547_02520"
     CDS             195..353
                     /locus_tag="VF547_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX8261728.1"
                     /translation="MQQAHMSALQHRHADIEARILEESQRPLPDMATLSRLKKEKLKL
                     KEAIAGIH"
     gene            complement(601..1056)
                     /gene="dksA"
                     /locus_tag="VF547_02525"
     CDS             complement(601..1056)
                     /gene="dksA"
                     /locus_tag="VF547_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX8261729.1"
                     /translation="MATALSEVYGSAYASGGFPDSYRPSANEEFMNPVQLAYFRSKLL
                     GWKDEILRESQGTMASLKSGPIREADVTDRASSETDWAIELRTRDRQRKLISKIDSAL
                     RRIDTGEYGFCEVTGEPISLARLEARPIATMTVEAQERHERNERITRDD"
     gene            complement(1803..2465)
                     /locus_tag="VF547_02530"
     CDS             complement(1803..2465)
                     /locus_tag="VF547_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261730.1"
                     /translation="MLVELPLEVELLVDALVLVLADVDALVEEAELVVVLAKLDEDEV
                     VETVPLDVLVEDPPVDVEVELPPVELEVELPPVEVEVEPPLVLLELPPVEVELPPVEV
                     ELPPVDVDPPLVEVEPPLVELPPVEVELPPVELPPVEVEPPLVEVELPPLPPVEVLVD
                     AVKMALPLEPPKKPPLKKPPPKPEPKPPLPPITVTPPPLPPAIGGKGGSGGRGIAAPP
                     PG"
     gene            complement(2641..3135)
                     /locus_tag="VF547_02535"
     CDS             complement(2641..3135)
                     /locus_tag="VF547_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261731.1"
                     /translation="MRFKLSLLAGLALASLSAPALAAVVVGAVVKDTKGGEVGTITRV
                     EGANLVLKTDRHEVTLPVSSFTATDEGALFGMTRDELNAEVDKAKAAADAQLVVGATV
                     KGIAGAAVGTIEAVDAQFVTLKLISGTSIRLPRSAVAGGPNGPVTGMTAEQIEAAAKA
                     ASPS"
     gene            complement(3194..3637)
                     /locus_tag="VF547_02540"
     CDS             complement(3194..3637)
                     /locus_tag="VF547_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment family protein"
                     /protein_id="HEX8261732.1"
                     /translation="MQVPHNSFVVVADGKKMLFFRNEGDSEFPKLEVERKRETSDAPD
                     RDRKTDGPGRTFDASGGAGRSAYQEVDFHQLEEERFAAETAELLRKRALRNDFESLII
                     VAPPKTLGELRKHYHKEVEKRLAGEVAKDLTGHPVTEIEKILQSA"
     gene            3728..4492
                     /gene="surE"
                     /locus_tag="VF547_02545"
     CDS             3728..4492
                     /gene="surE"
                     /locus_tag="VF547_02545"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX8261733.1"
                     /translation="MRILLTNDDGVNASGLRVLERIAARFSDDVWIVAPTEEQSGAGH
                     SLTLTRPVRLRKLGERRFCVTGTPTDAVMMAVAHLMKEAPPDLVLSGVNRGANLGEDV
                     TYSGTVSAAMEGAMAGVPAIALSQAYAREGMGDTVPFAAAEAWAERVLRPIIEGGMPP
                     RTLVNVNFPALPGEAVKGIRVAQQGRHDYGRLRIVKRTDPRGYDYFWFGLAPMVETPG
                     HSTDLEVVADGYVAVTPLHLDLTHAPSLARLAELYG"
     gene            4492..>4832
                     /locus_tag="VF547_02550"
     CDS             4492..>4832
                     /locus_tag="VF547_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX8261734.1"
                     /translation="MPHRRPRGPDEVNMRRRGRLPVWAQLAWRAALVVGLLAFTIGLH
                     WFEREGLKDTHDGHISFADVVYFTMISITTTGYGDIVPVAAHTRLYDALVVTPIRIFF
                     VLIFIGTAYTFV"
BASE COUNT          778 a         1706 c         1544 g          804 t
ORIGIN      
        1 cgcgacgccg ctgggatggc attgccctcg atcgcttcca tggcgctcct tggacgctac
       61 tcaaacgctt cgaaaggcgc tatagatagt ccattaacgg cccgtcgtac ataatgcggc
      121 ggccatcgga tcgatgggag acagaccacg cctccggacg ttgatcaccc tacccaacag
      181 cctaggagat gggaatgcaa caggcgcata tgtcggcatt gcagcaccgc cacgcggata
      241 tcgaagcgcg gatcctcgaa gaaagtcagc ggcctttgcc ggacatggcc accctctcca
      301 ggctcaagaa ggaaaagttg aagctcaagg aagcgatcgc cgggatccac taggcgaatc
      361 acagcgcgca tctcgcccgc gacgagccga tctcgaatcg gctcgaacgg gggcattaag
      421 ctaaaatgaa ccgttcgggc tgagcctgtc gaagcccctc ccttcgccgc ggccgcaagg
      481 gcaggaaggg cttcgacaag ctcagcccga acgggtgttc gagatccgac gggcttgatg
      541 cccggctttc acgccgatca tccgaataag ccggatcgaa gccgaacttc gatcgaagga
      601 tcaatcgtcg cgggtgatgc gctcgttgcg ctcgtgccgc tcctgggcct cgaccgtcat
      661 cgtcgcgatc ggccgcgcct cgagccgggc cagcgagatc ggctcgccgg tcacctcgca
      721 gaagccatat tccccggtgt cgatccggcg aagcgccgag tcgatcttgg agatgagctt
      781 gcgctgccgg tcgcgcgtgc ggagctcgat cgcccagtcg gtttccgagg aagcgcggtc
      841 ggtgacgtcc gcctcgcgga tcgggccgct cttcagcgac gccatcgtcc cctgcgattc
      901 gcgcaggatc tcgtccttcc agccgagcag ctttgacctg aaataggcga gctgcaccgg
      961 gttcatgaac tcttcgttcg cactcggccg gtagctgtcc ggaaaacctc ccgaagcata
     1021 agccgagccg taaacctcac tcaaagccgt cgccataacc cctccactcc tcaacgcgcc
     1081 gcgcccagcg gtcacgctac ctcctgagct ccgcttccga gtcgtccgga aatgtcgaag
     1141 cttgccccag cgcgcctata cgcctcgcaa aggggcagta caagtcactc aaatagcggt
     1201 taaaacttca cgccgattcg cgcatcgggc gtgacttttt tgccccgctg tccgagctcc
     1261 ggcccgttac gcctggggtc ccggccaaca gactccgaat cgggagccgg ccgagcgtta
     1321 accatgtttg tccgccccag cccggcagct attcgttcat gccttacccg cccttgtcca
     1381 caattgggac ggaagcggcc ccaacgggca ttttgcgggc attccgaggt gacttcgtgg
     1441 ttaacgcgct tgcacttaaa gctttccttt gcatctgtgt gcgtagctcg atgccgacgc
     1501 cgatcttccg acagggactt tcggctacgc atcggaacct gggaaacaag gtgcgctgtc
     1561 atgtcggtaa aaatggctct cgctaaactc tgtgcctgcg ccgccggtgg cgcgatcctc
     1621 ggcggaggcg ccgtccatgt cgcggagaac ccgccgcagc ggccgtcctt cgtcaagcac
     1681 gccaagatgg tgaagacggc accgcggcgc tacgccaggg tcaagccggt caagcgctac
     1741 aagagggtgc ggacggcctg cgtcgtcaag cccggcacga ccaccaccac ccgcacggtg
     1801 agctatccgg gcggcggcgc ggcgatgccc cttcccccgc ttccgccctt gcccccgatg
     1861 gccggcggca gcggcggcgg agtgaccgtg atcggcggca gcggcggctt cggctccggc
     1921 ttcggcggcg gcttcttcag cggcggcttc ttcggcggct ccagcggcaa cgccatcttc
     1981 accgcgtcga ccagcacctc gaccggcggc agcggcggaa gctcgacctc caccagcggc
     2041 ggctcgacct ccaccggcgg aagctcgacc ggcggaagct cgacctccac cggcggaagc
     2101 tcgaccagcg gcggctcgac ctcgaccagc ggcggatcga cgtcgaccgg cggaagctcg
     2161 acctccaccg gcggaagctc gacctcgacg ggcggaagct cgagcagcac cagcggcggc
     2221 tcgacctcga cctcgaccgg cggcagctcc acttcgagct cgaccggcgg cagctcgacc
     2281 tcgacgtcca cgggcgggtc ctcgaccagc acgtcgagcg gcaccgtctc gacgacctcg
     2341 tcttcgtcca gcttcgcgag cacgaccacc agctccgcct cttcgaccag cgcgtcgacg
     2401 tcggcgagca ccagcaccag cgcgtcgacc agcagctcga cctcgagcgg cagctcgacc
     2461 agcacgtcga gcggcggcca cgacgtgccg gcgccgccga tggtcctgct gttcggagcg
     2521 gcggccgcgg ccctggtcgc gcgtcgcagg atctcccgcg ccggcgactg atcgcgccgg
     2581 ccggacagcg aaaaggccgc gggacccttc gcccgcggcc ttttccatgt ctgccgagtc
     2641 ctaggagggc gaggcggcct tcgccgcagc ctcgatctgc tccgcggtca tccccgtcac
     2701 cggcccgttc gggccgccgg ccacggcgct tcggggaagc cggatgctgg tacccgagat
     2761 cagcttcagg gtcacgaact gggcatcgac cgcctcgatc gtgccgaccg cggcgccggc
     2821 gattcccttc acggtcgctc cgacgacgag ctgggcgtcg gccgcggcct tggccttgtc
     2881 cacctcggcg ttgagctcgt cccgggtcat tccgaagagg gcgccctcgt cggtcgcggt
     2941 gaagctcgac accggcaggg tgacctcgtg ccggtcggtc ttgaggacga gattggcgcc
     3001 ctcgacccgg gtgatcgttc cgacctcgcc gcccttggtg tccttgacga cggcgccgac
     3061 gacgaccgcg gccaaggccg gcgcggagag ggaagcgagc gcgaggccgg cgagaagact
     3121 gagcttgaaa cgcattgtca ctcctggatg gttcggggtt gagggacgcc cccggccgaa
     3181 gccggccttc aggctaggcc gactgcagga ttttctcaat ctcggtgacc ggatggccgg
     3241 tcaggtcctt ggcgacctcg ccggcgaggc gcttctcgac ctccttgtgg taatgcttgc
     3301 gcagctcgcc cagggtcttg ggcggggcga cgatgatcag cgattcgaaa tcgttcctca
     3361 gcgcccgctt cctcagcagc tcggcggtct cggcggcgaa cctctcctcc tcgagctggt
     3421 ggaagtcgac ctcctggtag gcgctccgcc cggcgccgcc cgaagcgtcg aaggttcggc
     3481 cgggaccgtc ggtcttgcgg tcgcggtccg gagcgtcgct cgtctcgcgc ttgcgctcga
     3541 cctccagctt ggggaattcg gaatcgccct cgttgcggaa gaacagcatc ttcttgccgt
     3601 cggcgacgac cacgaagcta ttgtggggaa cctgcatcgc tctctccttc ttcggaccga
     3661 ccaactcccg aatcgcggcg agggttgcgc gcttgcgcga caccgagcgg cgtcctagcg
     3721 ctcgcacatg cgcatcctcc tcaccaacga cgacggcgtg aacgccagcg gactgagggt
     3781 gctcgagcgg atcgcggcgc gattttccga cgatgtctgg atcgtcgccc cgaccgagga
     3841 gcagtccggc gcgggccatt cgctcaccct cacccgcccg gtgcggctga ggaagctggg
     3901 cgagcggcgc ttctgcgtca ccggaacacc gaccgacgcg gtgatgatgg cggtcgcgca
     3961 cctgatgaag gaagccccgc ccgacctcgt cctgtcggga gtcaaccgag gcgccaatct
     4021 cggcgaggac gtcacctatt cgggcaccgt ctcggcggcg atggaagggg cgatggcggg
     4081 ggtcccggcg atcgccctca gccaggccta tgcccgggaa ggcatgggcg acacggtgcc
     4141 gttcgccgcc gccgaggcct gggcggaacg cgtgcttcgg ccgataatcg agggcggcat
     4201 gccgccgcgc accctcgtca acgtcaactt cccggccctt ccgggcgagg cggtgaaggg
     4261 gatccgggtc gcccagcagg gccggcacga ttacgggcgg cttcggatcg tcaagcgcac
     4321 cgacccgcgc ggctacgact atttctggtt cgggctggcg ccgatggtcg agactccggg
     4381 ccattcgacc gacctcgagg tggtcgccga cggctatgtc gcggtcactc cgctgcatct
     4441 cgacctcacc cacgcgccct cgctggcgcg gctggcggag ctttacggct gatgccccac
     4501 cggcgcccgc ggggacccga cgaggtcaac atgcggcgcc gcggccgcct cccggtctgg
     4561 gcgcagctcg cctggcgggc cgccctcgtc gtcggtctac tcgccttcac catcgggctc
     4621 cactggttcg agcgcgaggg cctaaaggac acgcacgacg gccacatcag cttcgccgac
     4681 gtcgtctatt tcacgatgat cagcatcacc accaccggct atggcgacat cgtgccggtc
     4741 gccgcccaca cccggctcta cgacgcgctg gtggtgaccc cgatccggat cttcttcgtc
     4801 ctcatcttca tcggcaccgc ctacacgttc gt
//
LOCUS       DASVQZ010000091         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_885774, whole genome shotgun sequence.
ACCESSION   DASVQZ010000091 DASVQZ010000000
VERSION     DASVQZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885774"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VF547_02555"
     CDS             complement(<1..196)
                     /locus_tag="VF547_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX8261735.1"
                     /translation="MADLFASDPPPPPSGQAALPAGAPLADRLRPAKLDEVVGQGHLT
                     GPEGAIERMVKAGKLASMILW"
     gene            632..1279
                     /locus_tag="VF547_02560"
     CDS             632..1279
                     /locus_tag="VF547_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8261736.1"
                     /translation="MGLRFNLGPGGIGFDLGPGGFGPRPPRPPRGPGGFGGGFGDWGD
                     WSGDWGGGRGRRRRRQVDASELRLVLLHMIAAEPRHGYDLIRQFEELSGGEYAPSPGT
                     VYPMLTMLGDMGHIEEVPGEGTRKVFRATEEGKAWLAERADEADALLERLTGLGAGSR
                     RAGGAPIGRAVSNLLASLWGRVTRDDMDEATLHDIAEVLDEAARRIERLKKGGEA"
     gene            complement(1325..2404)
                     /locus_tag="VF547_02565"
     CDS             complement(1325..2404)
                     /locus_tag="VF547_02565"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8261737.1"
                     /translation="MDGQAAHAPDATPLDAGRDRVARQKASLAASAGWLLGIAEYYCA
                     LVPPEGASASQRLRRAARTLAWTSPQQERLAFFADRYADLDAEAERALADAATSPDVL
                     ADIARRLEQAASEALTFYVALQSEAQRAEELSRTSAAEENQPSRLGARAEVGAAVRAR
                     LPEVGAVKVQAAGLELYRVPGFFSAGECAAIVELIERDLYPSGVLGDSTDKEFRTSKS
                     CNLDRRDEIVGIFEDRVSALAGINPRFGESVQGQRYEVGQQFKAHFDFFHANESYYAD
                     AAARGGQRTWTAMLFLNEPEAGGHTDFPTAGVSAAPETGTLLVWNNMAPDGRCNEYAL
                     HHGTPVIAGRKYVLTKWFRERPWIF"
     gene            complement(2456..>3086)
                     /locus_tag="VF547_02570"
     CDS             complement(2456..>3086)
                     /locus_tag="VF547_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8261738.1"
                     /translation="DVGIWTRGIDRAIFHPGRRDLEWRRSLGVEDDVPVIGFVGRLVM
                     EKGLGVFADSIDRLEAGGVRHKVLVVGKGPAREWFENRLPAACFAGFQGGADLGRAVA
                     SMDMLFNPSVTETFGNVTLEAMAAGLPVVAARATGSESLVADGVTGRLIEAGDADAMA
                     DALAFYCRDEAARHAAGEAGERASRRFGWDEVNQALVDGYLRIVARHGR"
BASE COUNT          460 a         1014 c         1158 g          454 t
ORIGIN      
        1 cccacaggat catcgaggcg agtttgcccg ccttcaccat ccgctcgatc gcgccttcgg
       61 ggccggtcag atggccctgg ccgacaacct cgtcgagctt cgccggccgg agccggtcgg
      121 cgagcggcgc ccccgcgggc agggccgcct ggcccgaagg cgggggcggc gggtcggatg
      181 cgaagagatc ggccaccggg gcgctctagc ccaagcccga acgggcggcg agtcggcgcg
      241 ggctgcggct gccgaaaggt gacaaagctg gtgccgaaag ccggagaatc tcgtcacctt
      301 tgtcaacttc cggagcctgc gacgggtggt cggcaggggg cctgtcaggg gcactcgcgg
      361 cagcttggga cacaagggcg agcccagcac gacgaatcct attttggaag gggtcgatcc
      421 tccccgctcg cggggagagg gaccatgcgc agcatggtgg agggggggcg gagacttgtg
      481 ggagtgccag atgaagagcc ccctccacca ccctccccgt cccggggagg attgcccttc
      541 tccaagccac tgatatattg gagtaacaca gctcttgctt ccgcgagcgt ttcaagatat
      601 atcttgagcg actcacagca ggagcttgat catgggtttg cgcttcaacc tcggccccgg
      661 cggcatcggt ttcgaccttg gtccgggcgg cttcggccct cggccgccgc gccctccgcg
      721 cggacccggc ggtttcggcg gcggcttcgg cgactggggt gactggagcg gcgactgggg
      781 cggcgggcgg ggacggcggc gccggcgcca ggtcgatgca tccgagctga ggctcgtcct
      841 gctccacatg atcgcggccg agccgcgcca cggctacgat ctcatccgcc agttcgagga
      901 attgtccggc ggcgaatatg caccgagccc gggcaccgtc tacccgatgc tcaccatgct
      961 cggcgacatg ggccatatcg aggaagtgcc gggcgagggc acgcgcaagg tcttccgcgc
     1021 gaccgaggag ggcaaggcgt ggctcgccga gcgggcggac gaagcggacg cgctgctcga
     1081 gcgcctgacc ggtctcggcg ccggctcccg ccgcgcaggc ggcgccccga tcggccgggc
     1141 cgtatccaac ctgctcgcct cgctctgggg gcgagtcact cgcgacgaca tggacgaggc
     1201 gacattgcac gacatcgccg aggttctcga cgaagccgcg cgccggatcg agcggctgaa
     1261 aaagggcggg gaggcgtagg ggcgctgctg tccctctccc tcaaggggag aggggtagaa
     1321 gccttcaaaa gatccagggg cgctccctga accatttggt gagcacgtac ttgcggccgg
     1381 cgatgacggg cgtgccgtgg tggagggcgt attcgttgca gcggccgtcc ggagccatgt
     1441 tgttccagac gaggagggtg ccggtctcgg gcgccgcgga gacgccggct gtcgggaagt
     1501 cggtgtggcc gccggcctcc ggctcgttca ggaacagcat cgccgtccag gtgcgctgcc
     1561 cgccccgcgc cgccgcatcg gcataatagc tttcgttggc gtgaaagaag tcgaaatgcg
     1621 ccttgaactg ctgcccgact tcgtagcgct ggccctggac ggactcgccg aagcgcgggt
     1681 tgatgccggc cagggccgag acgcggtcct cgaagatgcc gacgatctcg tcccggcgat
     1741 cgaggttgca gcttttgctg gtgcggaact ccttgtcggt cgagtcgccg agcacgccgg
     1801 acgggtacag gtcgcgctcg atgagttcga cgatcgccgc gcactccccc gcggagaaga
     1861 agccgggcac acggtacagc tccagccctg ccgcctgcac tttgacggcg ccgacttcgg
     1921 gcagacgggc gcgaaccgcg gcgccgacct cggcgcgtgc gccgaggcgg gagggctggt
     1981 tctcttcggc ggcactggtg cggctcagtt cctcggcccg ctgcgcctcc gactgaagcg
     2041 cgacatagaa ggtgagggct tcggaggcgg cctgttcgag gcgccgggcg atgtcggcga
     2101 gcacgtccgg cgacgtggcg gcgtcggcca acgcgcgctc ggcctcggcg tcgagatcgg
     2161 cgtagcggtc ggcgaagaag gcgaggcgct cctgctgcgg gctcgtccag gccagagtgc
     2221 gggcggcgcg gcgcaggcgc tgcgaggccg aggcgccttc gggcggcacc agggcgcagt
     2281 aatattcggc gatgcccagc agccatccgg ccgaggcggc gagcgaggcc ttctgccgcg
     2341 cgacccggtc gcggccggca tccaaggggg ttgcgtcggg ggcgtgtgct gcttgtccgt
     2401 ccatcggcgc gttatggcat gacgcctcga ggggcggaag cgggtgcggc ggcgctcagc
     2461 ggccgtggcg ggcaacgatg cgcagatagc cgtcgacgag cgcctggttg acctcgtccc
     2521 agccgaagcg gcggctggcg cgctcgccgg cttcgcccgc cgcgtggcgc gcggcttcgt
     2581 cgcggcagta aaaagcgagc gcgtctgcca tggcgtccgc gtcgcccgcc tcgatcaagc
     2641 ggccggtgac gccgtcggcg acgaggctct cgctgccggt cgcgcgcgcc gccacgacgg
     2701 ggagaccggc ggccatggct tcaagggtga cgttgccgaa ggtctcggtg accgacgggt
     2761 tgaacagcat gtccatcgaa gcgacggcgc ggccgagatc ggcgccgccc tggaagccgg
     2821 cgaagcaggc ggcggggagg cggttctcga accactcgcg tgccggcccc ttgccgacga
     2881 cgaggacctt gtggcgcacg ccgcccgcct cgagccgatc gatcgaatcc gcgaagacgc
     2941 cgaggccctt ttccatgacg aggcggccga cgaagccgat gacaggaacg tcgtcctcga
     3001 cgccgaggct tcggcgccat tcaaggtcgc ggcggcccgg gtggaagatc gcgcggtcga
     3061 tgccgcgggt ccagatgccg acgtcg
//
LOCUS       DASVQZ010000092         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_787770, whole genome shotgun sequence.
ACCESSION   DASVQZ010000092 DASVQZ010000000
VERSION     DASVQZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787770"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2381)
                     /locus_tag="VF547_02575"
     CDS             complement(120..2381)
                     /locus_tag="VF547_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX8261739.1"
                     /translation="MRPEDSGRGLARLPRRLMAELALAEGDVIEVTGKRATPARAVGP
                     YAEDEGLDIIRLDGLQRANAEIGSGDFVTLRKVESKAAQRVVFAPAQKNLRLHGSAQA
                     LKRSFGMKPLTAGDVVATTGQQQIQRSDIPPELRQMLNAPAYALQEIRLTVVSTAPKG
                     VVHIDANTEVELRPEYAEATETRRADVTYDDLGGIGTTIDQLREMVELPLRYPEIFER
                     LGVDPPKGVLLYGPPGTGKTRLARAVANESDASFFHIAGPEIMGSAYGESERRLREVF
                     EEAAKASPAIIFIDEIDSIAPKRGQVQGETEKRLVAQLLTLLDGLEPRQNLVVIAATN
                     RPEALDEALRRPGRFDREIVIGVPDEGGRREILAIHTRGMPLDGGVDIKELARRTFGF
                     VGADLAALTREAAIEAVRRMMPQINLDEGTIPQEVLDNLSVKGSDFEEALKRVQPSAM
                     REVMVQAPTIRWDDIGGVDDARERLREGVELPLKSPEAFKRMGIRPAKGFLLYGPPGT
                     GKTLLAKATAREAEANFIATKSSDLLSKWYGESEQQIARLFARARQVAPTVVFIDELD
                     SLVPARGGGMGEPQVTERVVNTILAEMDGLEDLGSVVVIGATNRPNLIDPALLRPGRF
                     DELIYVAVPNEAGRRKILSIHTAKMPLAEDVDLDGFAARTDRFTGADLEDLVRRAGLF
                     ALRRSIDAVQVTGSDFEMAMEETRASVTPEMEKDYEKIQESLKRDAMSLDSGGIGFIS
                     PGMLTPRGPKGAD"
BASE COUNT          324 a          787 c          860 g          410 t
ORIGIN      
        1 gagggggacc gcgcgaagcg cggtggaggg gctctcttgg ggaagggggg cccctccacc
       61 attcttcgaa tggtccccct cccctgcaaa tgcaggggag gatttgaggg gctggcggct
      121 taatccgccc ccttcggccc cctcggcgtg agcattccgg gggagatgaa gccgattccg
      181 ccggaatcga ggctcatcgc gtcgcgcttc aggctttcct ggatcttctc gtaatccttc
      241 tccatctcgg gagtgacaga ggcgcgggtc tcctccatcg ccatctcgaa gtcggagccg
      301 gtcacctgca ccgcgtcgat cgagcggcga agcgcgaaga gtccggcgcg gcggacgaga
      361 tcctcgaggt cggcgcccgt gaagcggtcg gtccgggcgg cgaagccgtc gaggtcgacg
      421 tcctcggcga gcggcatctt cgcggtgtgg atggagagga tcttgcggcg gccggcctcg
      481 ttcggcaccg caacgtagat gagctcgtcg aaccggccgg ggcggagcag ggccgggtcg
      541 atcaggttcg ggcggttggt cgctccgatc acgacgacgg agcccagatc ctcgagcccg
      601 tccatctcgg cgaggatcgt gttgaccacc cgctcggtca cctgcggctc gcccattcct
      661 ccgccgcgcg cgggcaccag gctgtcgagc tcgtcgatga agacgacggt gggcgcgacc
      721 tggcgtgccc gggcgaagag gcgggcgatc tgctgctcgc tctcgccata ccattttgag
      781 agcaggtccg acgatttggt ggcgatgaag ttcgcctccg cctcgcgcgc cgtcgccttg
      841 gcgaggagcg tcttcccggt gccgggcggg ccgtagagga ggaagccctt ggccgggcgg
      901 attcccatcc gcttgaacgc ctcggggctc ttgagcggaa gctcgactcc ctcgcgaagg
      961 cgttcgcggg cgtcgtccac tccgccgata tcgtcccagc ggatcgtggg cgcctggacc
     1021 atcacctcgc gcatcgccga gggctggacg cgcttcaggg cctcctcgaa gtcgcttccc
     1081 ttgaccgaga gattgtccag cacctcctgc ggaatggtgc cttcgtcgag gttgatctga
     1141 ggcatcatcc gccggaccgc ctcgatcgcc gcctcgcggg tcaaagcggc gagatcggct
     1201 ccgacgaacc cgaaggtgcg gcgggcgagc tccttgatgt ccaccccgcc gtccaagggc
     1261 attccgcggg tgtggatggc gagtatctcg cggcggccgc cctcgtcggg aaccccgatg
     1321 acgatctcgc ggtcgaagcg gccgggccgg cggagcgcct cgtccaaggc ctcgggccgg
     1381 ttggtggcgg cgatgacgac gaggttctgg cgaggctcga ggccgtccag gagggtgagg
     1441 agctgggcga cgaggcgctt ctctgtctcg ccctggacct ggccgcgctt gggggcgatc
     1501 gaatcgatct cgtcgatgaa gatgatcgcg ggcgaggcct tggccgcttc ctcgaacacc
     1561 tcgcggaggc gccgctcgct ctcgccatag gccgagccca tgatctccgg cccggcgatg
     1621 tggaagaagc tcgcgtcgct ctcgttggcg accgcgcggg cgagccgggt cttcccggtc
     1681 cccggcggtc cgtagagcag cacgcccttg ggcgggtcga ctcccagccg ctcgaagatc
     1741 tccggatagc gaagcgggag ctcgaccatc tcgcggagct ggtcgatcgt cgttccgatc
     1801 ccgccgaggt cgtcataggt gacgtcggct cggcgggtct cggtcgcctc ggcatattcg
     1861 gggcggagct cgacctcggt attggcgtcg atgtggacga cccccttggg cgcggtcgag
     1921 acgacggtca gccggatctc ctggagggcg taggccggcg cgttgagcat ctgccgcaat
     1981 tccggcggga tgtcgcttcg ctggatctgc tgctggccgg tcgtcgcgac gacgtcgccg
     2041 gcggtgagcg gcttcatccc gaagcttcgc ttgagcgcct gggccgagcc gtgcaggcgc
     2101 agattcttct gcgcgggcgc gaagacgacc cgctgcgccg ccttggattc gaccttgcgc
     2161 aacgtgacga agtcgccgga gccgatctcg gcattggctc gctggaggcc gtcgaggcgg
     2221 atgatgtcga gcccctcgtc ctcggcatag ggaccgaccg cccgggccgg cgtggcgcgc
     2281 ttgccggtga cctcgatgac gtcgccctcg gcgagcgcga gctcggccat cagccgccgg
     2341 ggcaggcggg cgaggccgcg gccgctatcc tccggccgca t
//
LOCUS       DASVQZ010000093         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_117767, whole genome shotgun sequence.
ACCESSION   DASVQZ010000093 DASVQZ010000000
VERSION     DASVQZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117767"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VF547_02580"
     CDS             <1..141
                     /locus_tag="VF547_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine aminotransferase"
                     /protein_id="HEX8261740.1"
                     /translation="GAAYDVAGYRDAPPGIRIWCGATVETDDVEALTPWLDWAFHEAR
                     SS"
     gene            247..>1694
                     /gene="serA"
                     /locus_tag="VF547_02585"
     CDS             247..>1694
                     /gene="serA"
                     /locus_tag="VF547_02585"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX8261741.1"
                     /translation="MPKVLISDQMDPNAAEIFRANGVEVDEKPGLSKDELKAIIGDYD
                     GLAIRSATKVTKDVLEAATNLKVVGRAGIGVDNVDIPAASAKGVVVMNTPFGNSITTA
                     EHAIALMFALARELPQADASTQAGKWEKNRFMGVELTNKTLGLIGAGNIGSIVADRAL
                     GLKMKVIAYDPFLTPERAVDLGIEKVELDELLARADFITLHTPLTDQTRNILSRENIA
                     KTKKGVRIINCARGGLIDEEALKDALESGQVAGAALDVFREEPAKQSPLFGTPGFVAT
                     PHLGASTTEAQVNVAIQVAEQMSEFLTRGGVTNALNVPSLSAEEAPKLRPYMELAEKL
                     GSLVGQLAHGSLAGLSIEAEGAAAELNLKPITGAVLAGFMRIHSDTVNMVNAPFLAKE
                     RGLDVREIRHDREGDYHTLVRVSAQTEAGERSVSGTLFGNSAPRLVEIFGIKVEAELE
                     GDMIYIVNEDVPGFIGRLGSLLGESGVNIATF"
BASE COUNT          302 a          609 c          527 g          256 t
ORIGIN      
        1 ggcgcggctt acgatgtcgc cggctatcgc gacgcgccgc ccggcatccg catctggtgc
       61 ggcgccacgg tcgagaccga cgatgtcgag gctctgacgc cttggctcga ctgggccttc
      121 cacgaggcgc gcagctccta gttccggaga ccgtcacctc agcgaagctg tggtccatac
      181 ggcctctcca ccctttccga ttcagcatgg atcccggccc tcgccgggat gacgaggaat
      241 tccaaaatgc ccaaagtcct gatttccgac cagatggacc ccaatgccgc ggagattttc
      301 cgcgccaacg gagtcgaggt cgacgagaag cccggcctca gcaaggacga gctcaaggcg
      361 atcatcggcg actatgacgg cctcgccatc cgctcggcca ccaaggtgac caaggacgtc
      421 ctcgaagcgg cgaccaacct caaggttgtc ggccgcgccg gcatcggcgt cgacaatgtc
      481 gacatccccg ccgcctcggc gaagggcgtc gtcgtcatga acacaccctt cggcaactcg
      541 atcaccaccg ccgagcacgc catcgctttg atgttcgcgc tggcccgcga gcttccccag
      601 gccgacgcct ccacccaggc cggcaagtgg gagaagaacc gcttcatggg cgtcgagctc
      661 accaacaaga cgctcggcct gatcggcgcc ggcaatatcg gctcgatcgt cgcggaccgg
      721 gcgctcggcc tcaagatgaa ggtgatcgcc tacgatccgt tcctgacgcc ggagcgggcg
      781 gtcgacctcg gcatcgagaa ggtcgagctc gacgagctgc tcgcccgcgc cgacttcatc
      841 accctccaca cgccgctgac cgaccagacc cgcaacatcc tcagccgcga gaacatcgcc
      901 aagaccaaga agggcgtccg gatcatcaac tgcgcccgcg gcgggctgat cgacgaggag
      961 gcgctgaagg acgcgctgga gagcggccag gtcgccggcg ctgcgctcga cgtcttccgg
     1021 gaggagcctg cgaagcagag cccgctcttc ggcacgcccg gcttcgtcgc cacaccgcac
     1081 ctcggcgcct cgaccaccga ggcgcaggtc aacgtcgcca tccaggtggc cgagcagatg
     1141 tcggaattcc tgacccgcgg cggcgtcacc aacgcgctca acgtcccctc cctctcggcg
     1201 gaggaggcgc cgaagcttcg cccttatatg gagcttgccg agaagctcgg ctccctggtc
     1261 ggccagctcg cccacggctc gctcgcgggc ctctcgatcg aagccgaggg cgctgcggcc
     1321 gagctcaacc tgaagccgat caccggcgcc gtgctggccg gcttcatgcg catccattcg
     1381 gacacggtga acatggtcaa cgcgccgttc ctggcgaagg agcgcggcct ggacgtccgc
     1441 gagatccgcc acgaccgcga gggcgactac cacaccctcg tccgcgtctc ggcccagacc
     1501 gaggcgggcg agcgctcggt gtctgggacc ctgttcggca attcggcgcc gcgcctggtc
     1561 gaaattttcg gaatcaaggt cgaggccgag ctcgagggcg acatgatcta catcgtcaac
     1621 gaggacgtac ccggcttcat cggccggctc ggctcgctgc tcggcgaatc gggggtgaac
     1681 atcgccacct tcca
//
LOCUS       DASVQZ010000094         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_405186, whole genome shotgun sequence.
ACCESSION   DASVQZ010000094 DASVQZ010000000
VERSION     DASVQZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405186"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="VF547_02590"
     CDS             complement(<1..1550)
                     /locus_tag="VF547_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX8261742.1"
                     /translation="MTDLHKILSATRPMTLAGAPAGFLPWLAADLARAAKGRAVFVAP
                     DEAAMRHLQDAAAYFAPELEVLAFPAWDCLPYDRASPSLRASSERLATLHALQRKAGK
                     PQLLVTTVNAVTQRTLTPFRVRQLVARLAPGERIDMARLSALLSANGYHRTDTVQDAG
                     EYAVRGGIVDLFPAGESEGLRLDFFGDEIESVRRFDPATQRTTGNEAGGFTLLPASET
                     LLDDDSVKRFRSRYRERFGATATGDPLYQAVSEGRRLAGLDHWLPLFEERLDTLFDHL
                     GDDDLVVRDAHDAGAADARFEAIADYYENRKRAQSSDPGSYRPLEPETLYLSREEWDG
                     LIAARPLHLATPFHEPESRTVVDFGVDAARDFAPERSQGGNVYEAVVEHVAALRKAGK
                     KVVLASYSAGARERLKGLLADHGLKKAVEVDTWQEALGASAPPRNGEGDHAKHGGGGP
                     EAGTPPPASGRSPSPSRGGTIALAVLPLDHGFTTSDVALLTEQDMLGDRLVRRRKRRK
                     SADAFLSEL"
     gene            complement(1551..1817)
                     /locus_tag="VF547_02595"
     CDS             complement(1551..1817)
                     /locus_tag="VF547_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX8261743.1"
                     /translation="MDRDDRLKRLHFRAWHRGTREADFLIGGFFDAHGSGWGESEMDW
                     FEALLEEQDVDIMAWALGTEPPPERYAGGQMRRLQDLNYVKHHR"
     gene            2046..>2691
                     /locus_tag="VF547_02600"
     CDS             2046..>2691
                     /locus_tag="VF547_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX8261744.1"
                     /translation="MRPDILNPLFAEVEVLKGIGPALAKPLKRLGLNRVLDLLFHLPV
                     SWIERKKVDVLDMADAGGVVTVVVTPVDYRQGGPRSPFRVYAADRAGNYLSLTYFHNP
                     GWAKKQFPLGVPKVVSGRMEMYGQELQMVHPDYVLEPKEALDLPEREPVYPLSEGLTN
                     KRMADLAGQALARAPALAEWIEPSLLAARGWPSWQEALGSVHHSRSAGPARERLA"
BASE COUNT          384 a          927 c          981 g          399 t
ORIGIN      
        1 agctcggaga ggaacgcgtc ggccgacttg cgccgcttgc ggcggcgcac gagccggtcg
       61 ccgagcatgt cctgctcggt cagcagggcg acgtccgacg tggtgaagcc atggtcgagc
      121 ggaagcacgg cgagggctat cgttcctccc cgggacgggg agggggaccg gccggaggcc
      181 ggtggagggg tccccgcctc cgggccccct ccaccatgct tcgcatggtc cccctccccg
      241 ttccggggag gagcagaggc gccgagcgct tcttgccatg tatcgacctc gacggccttt
      301 ttaagcccat ggtcggcgag cagtcccttc agccgctcgc gcgctccggc ggaatagctc
      361 gccagcacga ccttcttgcc cgccttcctc agcgccgcga catgctcgac caccgcctcg
      421 tagacgttgc ccccctgcga gcgctccggc gcgaagtcgc gggcggcgtc gactccgaaa
      481 tcgaccaccg tcctgctctc gggctcgtgg aaaggcgtgg cgaggtggag cggccgcgcc
      541 gcgatcagcc cgtcccattc ctcccgcgag agatacaggg tctccggctc gagcggccgg
      601 tagctgccgg ggtccgagga ctgcgcccgc ttccggttct cgtaatagtc ggcgatcgcc
      661 tcgaagcggg cgtcggcggc gccggcgtcg tgggcgtcgc ggacgaccag gtcgtcgtcg
      721 ccgagatggt cgaacaaggt gtcgagccgc tcctcgaaca ggggcagcca atggtcgagg
      781 ccggcgaggc gccgcccctc cgacaccgcc tggtagagcg gatcgccggt ggcggtggcg
      841 ccgaagcgct cgcggtagcg ggagcggaag cgcttgacgc tgtcgtcgtc gagcagcgtc
      901 tcggaggcgg gaaggagggt gaagccgccg gcctcgttgc cggtggtccg ctgggtggcg
      961 gggtcgaagc ggcggacgct ctcgatctcg tcgccgaaga aatcgagcct gaggccctcg
     1021 ctctcgccgg ccgggaacag gtcgactatg cctccgcgaa ccgcatattc gccggcgtcc
     1081 tggacggtgt cggtgcggtg gtagccgttg gcgctgagca gcgccgacag cctcgccatg
     1141 tcgatccgct cgcccggagc gagccgggcg acgagctggc gcacccggaa cggggtcagg
     1201 gtccgctggg tgacggcgtt gacggtggtg acgagcagct gcggcttgcc ggccttgcgc
     1261 tggagcgcgt gaagcgtcgc cagccgctcg gacgaggcgc gcagggacgg ggaggcccgg
     1321 tcgtagggca ggcagtccca ggcggggaag gcgagcacct ccagctccgg ggcgaaatag
     1381 gccgccgcgt cctgcaggtg ccgcatcgcc gcctcgtcgg gggccacgaa cacggcgcgc
     1441 cccttcgccg cgcgcgccag gtcggcggcg agccagggca ggaagcccgc gggcgccccg
     1501 gccagggtca tcggccgagt cgcggagagg atcttgtgga ggtcggtcac ttatcggtgg
     1561 tgcttgacgt agttgagatc ctggagccgc cgcatctgcc cgcccgcgta gcgctccggc
     1621 ggcggctcgg ttccgagcgc ccaggccatg atgtcgacgt cctgctcctc gagcagcgcc
     1681 tcgaaccagt ccatctccga ctcgccccag ccggagccgt gggcgtcgaa gaagccgccg
     1741 atcaggaagt cggcttcccg ggtgccgcga tgccaggcgc ggaaatggag gcgtttgagg
     1801 cggtcgtcgc ggtccatggg gtccatgcgg aaaaggccgc cgggagaatc cgggcggctg
     1861 gtgccggggc gatgtagggt cggcgagccg gccgctcaat cccccctccc tgcacgcagg
     1921 gaggggcggg gggtgggtac ttctccggaa atgcactgcc gcatctgacg cacccacccc
     1981 tgacccctcc ctgcgtgcag ggagggggat ttctacagcg cggctccgca cgctatggac
     2041 ccctcatgcg gcccgacatc ctcaacccgc tcttcgccga agttgaagtg ctcaagggca
     2101 tcggaccggc gctcgccaag cccctgaagc gcctcggcct gaaccgcgtc ctcgacctcc
     2161 tcttccattt gccggtgagc tggatcgagc ggaagaaggt cgacgtgctc gacatggcgg
     2221 atgccggcgg agtggtcacc gtcgtcgtca ctccggtcga ctaccgccag ggcggaccgc
     2281 gaagcccgtt ccgggtctac gccgccgacc gcgccggcaa ttacctgtcg ctcacctatt
     2341 tccacaatcc gggctgggcg aagaagcagt tcccgctcgg cgttccgaag gtcgtgtccg
     2401 ggcggatgga gatgtacggc caggagcttc agatggtcca tccggactat gtgctggagc
     2461 ccaaggaggc gctcgacctg cccgagcgcg agccggtcta tcccctgtcc gaaggcctca
     2521 ccaacaagcg catggccgac ctcgccggcc aggccctggc gcgggcgccg gcgctcgccg
     2581 agtggatcga gccgagcctc ctggccgcgc ggggctggcc gtcctggcag gaggcgctcg
     2641 gaagcgtgca ccacagccgc agcgccggtc ccgcgcgcga gcgcctggcc t
//
LOCUS       DASVQZ010000095         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_308785, whole genome shotgun sequence.
ACCESSION   DASVQZ010000095 DASVQZ010000000
VERSION     DASVQZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308785"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VF547_02605"
     CDS             <1..1099
                     /locus_tag="VF547_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HEX8261745.1"
                     /translation="MLDLAIEGRVEGWRVHLDRLQDAADRTAAVTRAAYPELRIPFHA
                     RFRHFVAGPPRLPKGNPQERGRAAFDLVILSVLLDAGSGPGWRYTDSTSGKTFTRSEG
                     LAVASQRMFESGALSDIASLTTEALAQGFQVSDDNPLAGLEGRANLLRRLGAQVAARP
                     DLFPGGRPGGLFDVLAARAGGGPLPAPAVLELLLEALGPVWADRLEVSGVALGDCWRH
                     PAIRRDDATDGLVPLHKLSQWLSYSLIEPLQEGGIEVVEIDGLTGLAEYRNGGLFVDT
                     GVLTLADAEDASRPHNVSDPLIVAWRAMTVALLDRIAPLVRARLGVSEADFPLARMLE
                     GGSWAAGRQIAAELRPGGGPPFTIISDGTVF"
     gene            1102..1731
                     /gene="upp"
                     /locus_tag="VF547_02610"
     CDS             1102..1731
                     /gene="upp"
                     /locus_tag="VF547_02610"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX8261746.1"
                     /translation="MDGLTIVSHPLVQHKLTILRDRTSSIQLFRTVVRELATLLCYEV
                     TRDLPVERVPVETPIASAELPAIAGKKLVFAPILRAGLAMAEGMLDLVPSARVAHIGL
                     YRDPATLEAIEYYFKTPEDVADRRVIVVDPMLATGHTATAAIDRLKEAGVTDMRFVCL
                     LAARPGAEALREAHPDVPIWAAGMDEELNEHGYIVPGLGDAGDRTYGTR"
     gene            complement(1852..>2509)
                     /locus_tag="VF547_02615"
     CDS             complement(1852..>2509)
                     /locus_tag="VF547_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8261747.1"
                     /translation="SNTLLISIGVPPAAASAGVHTVESFTTAVSGISHVAHRNVDWNL
                     FGRLVIPGVMGGVIGAYLLTNIHADTARPFVLAYLSCIGVYILWRGLRHEHVDKRPRH
                     VAPLGLAGGFLDAAGGGGWGPVVTSNLLIQGSDPRRTIGTVNTAEFFLTVAISATFIA
                     ALGWAAFTTATVGLLIGGVLAAPLGAFAAKRVAARHLLVLVGGVLLVTSLYGIWRALS
                     "
BASE COUNT          381 a          889 c          866 g          373 t
ORIGIN      
        1 gatgctcgac ctcgcgatcg aggggcgggt cgagggatgg cgcgtccacc tcgaccgctt
       61 gcaggacgcc gccgaccgca cggcggcggt gactcgcgct gcctatcccg aactgcgcat
      121 cccgttccac gcgcgcttcc ggcacttcgt cgccggtccg ccgaggctcc ctaaaggcaa
      181 tccgcaggag cgcggccggg cggcgttcga cctcgtcatt ctctcggtcc tgctcgacgc
      241 cgggtcgggc ccgggctggc gctacacgga cagcacaagc ggcaaaacct tcacccgctc
      301 ggaaggcctc gccgtcgcga gccaacgcat gttcgaatcc ggtgcgctga gcgatatcgc
      361 ttcgctcacc accgaggcgt tggcgcaggg cttccaggtc tcggacgaca atccgctcgc
      421 cggcctggaa ggccgcgcga acctcctgcg ccgcctcggc gcgcaggtcg cggcgcggcc
      481 cgaccttttt cccggcggcc ggcctggcgg cctgttcgac gtgctcgccg cgcgtgccgg
      541 cggcggcccg ctgcccgcgc ccgccgtcct cgagctgctg ctcgaagcgc tcggccccgt
      601 ctgggcggat cggctcgaag tgagcggcgt ggcgctgggc gattgctggc gccacccagc
      661 gatccggcgg gacgatgcca ccgacggcct cgtcccgctc cacaagctct cccaatggct
      721 gagctattcg ctgatcgagc cgctgcagga gggcgggatc gaggtggtgg agatagatgg
      781 cctgaccggt ctcgccgaat accgcaacgg cggcctgttc gtggacacgg gcgtgctgac
      841 cctcgccgac gcggaagacg catcgcgtcc gcacaatgtg tccgatccgc tcatcgtcgc
      901 ctggcgcgcg atgacggtgg cgctgctcga tcgcatcgcg ccgcttgtcc gcgcccgcct
      961 tggcgtctcc gaagccgatt tcccgctcgc ccgcatgctc gaaggcggga gctgggcggc
     1021 cggccggcag atcgcggccg agcttcgccc gggcggcggc ccgcccttca ccatcatcag
     1081 cgacggaacg gtattctaga catggacggc ctcacgatcg tttcgcaccc tctggtgcag
     1141 cacaagctca ccatccttcg cgaccggacg agctccatcc agctgttccg aaccgtggtg
     1201 cgagaactcg ccactttgct ctgctacgag gtgacgcgcg acctccccgt agagcgcgtt
     1261 cccgtcgaga cgccgatcgc atcggccgag ctcccggcca tcgccggcaa gaagctggtc
     1321 ttcgcgccga tcctgcgcgc cggcctcgcc atggccgaag gcatgctcga tctcgttcct
     1381 tccgcccgcg tcgcgcatat cggcctctac cgcgatcccg ctaccctcga agccatcgaa
     1441 tattacttca agacgcccga ggacgtcgcc gaccgacgcg ttatcgtcgt cgatccgatg
     1501 ctcgccaccg gtcacaccgc caccgccgcg atcgaccgcc tgaaggaagc gggcgtcacc
     1561 gacatgcgct tcgtctgcct cctcgctgcc cgcccgggcg ccgaggcgct ccgcgaggcg
     1621 catccggacg tgccgatctg ggcggccggg atggacgagg agctgaacga gcacggctat
     1681 atcgtccccg gcctcggcga cgccggcgac cggacctacg ggacgcgcta agggcggggc
     1741 gcggcaaacc gcccttcgac aggctcaggg ctggcggggt ggtcagggct accgggtgca
     1801 acaaaccccg ccagggctga gcctgtcgaa gccggtgtgc agcgcgcaaa ctcaggaaag
     1861 cgcgcgccag atcccgtaga ggctggtgac gaggagcacg cccccgacga ggacgagcag
     1921 gtgcctggcc gccaccctct tcgcggcgaa ggcgcccagc ggcgcggcga ggacgccgcc
     1981 gatcagcagg cccaccgttg ccgtcgtgaa cgcggcccag cccagcgcgg cgatgaaggt
     2041 ggccgagatc gcgacggtga ggaagaattc ggcggtgttg acggtcccga tcgtccggcg
     2101 cggatccgat ccctggatga ggaggttcga ggtgaccacc ggcccccagc cgccgccgcc
     2161 ggccgcatcg aggaagcctc cggcgaggcc gagcggggcg acgtgcctgg gccgcttgtc
     2221 gacatgctcg tgcctgaggc cgcgccagag gatgtagacg ccgatgcagc tcagataggc
     2281 gaggacgaag gggcgggcgg tgtcggcgtg gatgttggtg aggagatagg cgccgatcac
     2341 tccgcccatc actcctggaa tgacgaggcg cccgaacaga ttccagtcga cgttgcggtg
     2401 cgcgacatgg ctgatgccgg agacggcggt ggtgaagctt tcgaccgtgt gaaccccggc
     2461 cgaggcggcg gcgggcggaa cgccgatgct gatgaggaga gtgttcgag
//
LOCUS       DASVQZ010000096         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_118131, whole genome shotgun sequence.
ACCESSION   DASVQZ010000096 DASVQZ010000000
VERSION     DASVQZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118131"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..809
                     /locus_tag="VF547_02620"
     CDS             246..809
                     /locus_tag="VF547_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8261748.1"
                     /translation="MITRIREVRRAKKLTLQNVADRCDPPTTPQTIGRLETGTRTVSV
                     GWLNRIARALGVEATDLVHQPERDDVPLAAIIGDRGAHAPRRDGVALPPQPQPGTVAV
                     AVESSVGDYRSGDEIWCLRLAPDDFGRALNRDVLVPRPAGRFLFGRLIGREEGKLHLL
                     PLGAARRQLVIADPPWMAMASRLIRNL"
     gene            complement(1009..1084)
                     /locus_tag="VF547_02625"
     tRNA            complement(1009..1084)
                     /locus_tag="VF547_02625"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1049..1051),aa:Val,seq:tac)
     gene            complement(1126..2064)
                     /locus_tag="VF547_02630"
     CDS             complement(1126..2064)
                     /locus_tag="VF547_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX8261749.1"
                     /translation="MTAVATDPAYQALEWIEYRAGPAVLVLADEPAGERWREAARSAG
                     CRVIDVVPIAKAADRLDRQVAADAVLLDCPDDRAGLLDPLLRRLADAADAWRFGSVVA
                     TPMALVDRVAAQVFVPGIEQLCEPTQGERVAAVRRVAAHHPSQVHDVNKAQATLRLQQ
                     LSEEVGRIANVLAALSEDEAAAAAMAAVGAGPNGEGERLDGAFIRSIIRARRLRDHFF
                     QGELFADPAWDMLLDLMAARVERQRVAVSSLCIAAAVPPTTALRWIKTLCDQGLFVRV
                     ADPEDGRRVFIELSVETATMMEAYLRSAQRLAPLLV"
     gene            complement(2314..3009)
                     /locus_tag="VF547_02635"
     CDS             complement(2314..3009)
                     /locus_tag="VF547_02635"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX8261750.1"
                     /translation="MRKLGMIGGTSWLSTAMYYEQINKAVAKARGGLSSAPLVIESLD
                     LAPIAEWELAGAWRRVEEAIAAAAARLEAAGAEGLVICSNTTHKVAPAVEAAVGVPLL
                     HIADAAAERLAADGVKKAGLIGTRFAMNEPFLRERIEARGIALALPDAATAAEIDRII
                     FEELAKGQVTRSSQRRLKTCLTEMGKARQQAVILGCTELVLLVDPGASVIPLYDTTAI
                     HAAAAARWILDEF"
BASE COUNT          442 a         1129 c         1025 g          467 t
ORIGIN      
        1 cagccgagcg gcgattcggc gaggttgacg gtgaccgagc gcgccgccgc ccggccgcgg
       61 cccttgccgc gctcgcgccc gtcctggggc gccggccgct ccgccagcag ccgattgcgc
      121 cgcgcgcgct ccgtcttcat cgccccgtcc ccgtatcagt acataacacg aacatcttgc
      181 catggcgcgc cggatgtagg aaggtggaaa aagaaccgat aaggtttctg atccggggaa
      241 ttgagatgat cacccgaata cgggaggtgc gccgcgccaa gaagctcacc ctccagaacg
      301 tcgccgaccg ctgcgatccg ccgacgacgc cgcagaccat cggccggctc gagacgggca
      361 cgcgcacggt ctcggtcggc tggctcaacc ggatcgcccg tgcgctcggc gtggaggcga
      421 ccgacctcgt ccaccagccc gagcgcgacg acgtgccgct cgccgccatc atcggcgacc
      481 gcggcgccca tgccccccgc cgcgacggag tcgccttgcc gccgcagccc cagcccggga
      541 cggtcgcggt ggcggtcgaa tcgagcgtcg gcgactatcg cagcggcgac gagatctggt
      601 gcctgcgcct ggccccggac gacttcggcc gggcgctcaa ccgcgacgtg ctcgttcccc
      661 gccccgccgg ccgcttcctg ttcggccgcc tgatcggccg cgaggaaggc aagctccacc
      721 tcctcccgct cggcgccgcc cgccggcagc tggtcatcgc cgacccgccc tggatggcga
      781 tggcgtcgag gctgatccgg aacctctgaa gcgcggtcgg gttggagcga tccggaccgc
      841 gtgaaggact cgcgtccggg ccccgtccta ttgccggcag gagcctttcc gccccatgtt
      901 cctcttcttc tccaaccgcc tcggctgcgg cggctcgctg ctcgtcaccg cgctcggcac
      961 gctggtcgtc ctctggctgc tcggctggct gcgcttctga aaggcgggtg gtgggcgctg
     1021 acgggctcga accgccgaca tcttccgtgt aaaggaagcg ctctaccaac tgagctaagc
     1081 gcccgcgccg gggcctgtgc caccgcgggc ggacagcggc aagcctcaga cgaggagcgg
     1141 ggcgaggcgc tgggcggacc tgagataggc ctccatcatc gtcgcggtct cgaccgacag
     1201 ctcgatgaag acgcggcggc cgtcctcggg gtcggcgacc cggacgaaca ggccctggtc
     1261 gcagagcgtc ttgatccacc tcagcgccgt cgtcggcggc accgccgcgg cgatgcacag
     1321 gctcgagacg gcaacgcgct ggcgctccac cctcgcggcc atcaggtcga gcagcatgtc
     1381 ccaggcggga tcggcgaaca gctcgccttg gaagaaatgg tcgcgaagcc ggcgcgccct
     1441 tatgatcgag cggatgaagg cgccgtcgag ccgctcgccc tcgccattgg gaccggcgcc
     1501 gacggcggcc atggcggcgg cggcggcctc gtcctcggac aaggcggcga ggacgttggc
     1561 gatccggccg acctcctcgc tcagctgctg aaggcgaagc gtggcctggg ccttgttgac
     1621 gtcgtgcact tgcgacggat gatgggccgc gacccggcgg accgccgcga cccgctcgcc
     1681 ctgggtcggc tcgcagagct gctcgatccc cggcacgaag acctgggcgg cgacccggtc
     1741 gaccagcgcc atcggagtgg cgacgacgct gccgaagcgc caggcgtcgg ccgcatcggc
     1801 gagccgacga agcagggggt cgagcaagcc cgcccgatcg tccgggcagt cgagcagaac
     1861 ggcatcggcg gcgacctgcc ggtcgagacg gtcggcggcc tttgcgatcg ggacgacgtc
     1921 gatgacccgg cacccggcgg accgcgccgc ttcccgccat cgctcgccgg cgggctcgtc
     1981 ggcgagcacg agcacggcgg ggccggcccg atactcgatc cattccagag cctgataggc
     2041 tgggtccgtc gccactgccg tcatcgccgt ccgctccccc aagcgcagat tgcgaccgtt
     2101 acggagcaat tacaggcccc acattggtcc attttggtgt ttcttacact tccgcggcgc
     2161 ggggggcgcc tgttttttgt cgaaggggtc ccgtccgtct cggtcaggac ccgccgcggc
     2221 ggccttggag cgcgcaaacc caaggccttg cgcgaatccc ggatagtccg ccgccggcgg
     2281 ctttcatgtc gtcggactcc atccaattcg gatctaaaat tcgtccaaaa tccaccgagc
     2341 cgccgcggcg gcatggatcg ccgtcgtgtc gtagagcgga atcacgctgg cgccggggtc
     2401 gacgagcagc accagctcgg tgcagccgag aatcacggcc tgctggcgcg ccttgcccat
     2461 ctcggtcagg caggtcttca ggcggcgctg cgagcttcgc gtgacctgcc ccttggccag
     2521 ctcctcgaag atgatccggt cgatctccgc ggcggtggcg gcatcgggca gagcgagcgc
     2581 gattcccctg gcttcgattc gctcccgaag gaacggctcg ttcatcgcga agcgcgtgcc
     2641 gatcagcccc gccttcttca ccccgtcggc cgccagccgc tcggccgcgg catcggcgat
     2701 atggagcagg ggcaccccga ccgccgcctc gaccgccgga gcgaccttgt gcgtggtgtt
     2761 gctgcagatg accaatccct cggccccggc cgcctcgagc ctcgccgccg cggcggcgat
     2821 ggcctcttcg accctgcgcc acgcgccggc cagctcccac tcggcgatcg gcgcgagatc
     2881 gaggctctcg atgacgagcg gcgccgagct cagcccgccg cgcgccttcg ccaccgcctt
     2941 gttgatctgc tcgtaataca tggcggtcga gagccagctc gtcccgccga tcatccccag
     3001 tttgcgcacg tttccattcc caaagcctcg ctcgcctcca gcttgccgga ccaggcttct
     3061 tca
//
LOCUS       DASVQZ010000097         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_498575, whole genome shotgun sequence.
ACCESSION   DASVQZ010000097 DASVQZ010000000
VERSION     DASVQZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498575"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /gene="tolB"
                     /locus_tag="VF547_02640"
     CDS             <1..500
                     /gene="tolB"
                     /locus_tag="VF547_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500982.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HEX8261751.1"
                     /translation="TDIYRVSAQGGAPVRLTTSPGIDVGGSYSPDGSKIVFESDRSGS
                     QQLYVMNADGSGQRRISFGGGGYATPEWSPRGDLIAFTRIAGNFRIGVMSPDGSGERL
                     LTDSWQDEAPSWSPNGRVLQFFRTSQGRGGKSSVWQVDLTGRNERRIPTPLDGSDPAW
                     GPILP"
     gene            527..1069
                     /gene="pal"
                     /locus_tag="VF547_02645"
     CDS             527..1069
                     /gene="pal"
                     /locus_tag="VF547_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX8261752.1"
                     /translation="MRQTPWKVAAIAAALALTAGCGKKAPKELPPAPQEETGGETSGA
                     GEDASAGGVGTEALPGSREAFLQAVGMGGDRVFFDTDQYDLDDRDRATLDAQAGWLQS
                     NPRVRVTIEGHADERGTRDYNLALGDRRANAAKNYLAARGVSPSRMTVISYGKERPEA
                     LGSDETAWAQNRRAVTVLPE"
     gene            complement(1243..2166)
                     /locus_tag="VF547_02650"
     CDS             complement(1243..2166)
                     /locus_tag="VF547_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8261753.1"
                     /translation="MTQVPWKLVIHGGAGVLERERSTPEQDREIREALDRALAAGSEI
                     LAAGGSALDAVERAACVLEDDPNFNAGRGSVFTYQGTNELDAAVMDGRDRDAGAVTGV
                     TATRNPVSLARAVMEHSPHVFLSREGADQFSRDHGLEQAEPEWFATAERRRQLEVMRS
                     QEDHYFDEELKYGTVGAVAVDVHGHVAAATSTGGLTGKRWGRIGDTPLIGAGTYADDR
                     AGAVSATGAGEYFIRAAAAHEICARMRMLGESARVAADKAIADVGALGGSGGVILVTP
                     AGDAAWAFNTPGMYRGIASAEGRRVAIYADE"
     gene            2391..>3216
                     /locus_tag="VF547_02655"
     CDS             2391..>3216
                     /locus_tag="VF547_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8261754.1"
                     /translation="MATSRHESPDHQALRLSRERPAHTYNGYAMLLALLAALVAMVLG
                     FGLAVRDGGGAGPVLLVLAALVALLILFGFYLLQPNQAAALLLFGDYKGTDRHSGLRW
                     RWPWLTRTKISTRVHNVTSERLKVNDLRGNPIEIATNVVWRVTDTAQALFDVDDYNKF
                     VFVQIESAVRAIAARYPYDDIEHQETTLRGHAETVSDELKEELQERVGVAGITIDECR
                     LTHLAYAAEIAQSMLRRQQAEAVIAARQKIVIGAVSMVENALQLLSEKGVVELDDER"
BASE COUNT          503 a         1165 c         1071 g          477 t
ORIGIN      
        1 ataccgacat ctaccgggtc tccgcgcagg gcggagcgcc ggtgcggctc accacctcgc
       61 ccgggatcga cgtcggcggt tcctattcgc cggacgggtc gaagatcgtc ttcgaaagcg
      121 accgctcggg cagccagcag ctgtacgtga tgaacgccga cggctccggc cagcgccgga
      181 tcagcttcgg cggcggcggc tacgcgacgc ccgaatggag cccgcgcggc gacctcatcg
      241 ccttcacccg gatcgccggc aacttccgga tcggggtaat gagccccgac ggctcgggcg
      301 aacgccttct gaccgacagc tggcaggacg aggcgccgag ctggtcgccc aacggccggg
      361 tcctccagtt cttccgcacc agccagggcc gcggcggcaa gtcgagcgtc tggcaggtcg
      421 acctcaccgg ccgcaacgag cgccggatcc ccaccccgct ggacggatcg gaccccgcct
      481 ggggcccgat cctgccctag atgcacccga ccgaaggaga gagcgtatga gacagacccc
      541 ctggaaggtc gcggcgatcg ccgcggcgct ggcgctgacc gccggctgcg gcaagaaggc
      601 ccccaaggag cttccgcccg cgccgcagga ggagaccggc ggcgagacga gcggcgccgg
      661 cgaggacgcc tcggccggcg gggtcggcac cgaggcgctg cccggctcgc gcgaggcctt
      721 cctgcaggcg gtgggaatgg gcggcgaccg cgtgttcttc gacaccgacc agtacgacct
      781 cgacgaccgc gaccgggcca cgctcgacgc ccaggccggc tggctgcaat ccaatccgcg
      841 cgtccgagtc accatcgagg gccatgccga cgagcgcggc acccgcgact acaatctcgc
      901 cctgggcgac cgccgcgcca acgccgccaa gaactatctc gccgcccgcg gcgtctcgcc
      961 gtcgcggatg acggtgatca gctacggcaa ggagcggccc gaggcgctcg gctccgacga
     1021 gaccgcctgg gcccagaacc gccgcgcggt gacggtgctc ccggagtaag atctccctct
     1081 ccccttcagg ggagagggcc ggggagaggg ggcgttcgac ctgctgcccc tctcctcccg
     1141 ccgcttcgcg gctccttcct ctcccctgaa ggggagaggg ttattgcaaa gggggacacg
     1201 taccttttcc cgaaaaaggt acgtgtcccc attctccgtt ttctattcgt ccgcgtagat
     1261 cgccacgcgg cgcccttccg ccgaggcgat gccgcggtac atgccggggg tgttgaacgc
     1321 ccaggcggcg tcgcccgccg gggtcaccag gatcacgccg ccgctgccgc ccagcgcgcc
     1381 gacgtcggcg atcgccttgt ccgcggcgac ccgggcgctc tcgccgagca tccgcatccg
     1441 cgcgcaaatc tcgtgggcgg cggcggcgcg gatgaaatat tcgccggcgc cggtcgccga
     1501 gaccgccccg gcgcggtcgt cggcataggt gcccgcgccg atcagcggcg tgtcgccgat
     1561 ccggccccag cgcttgccgg tgaggccgcc ggtcgaggtc gccgccgcga catggccgtg
     1621 gacgtccacc gcgaccgccc cgaccgtgcc gtatttcagc tcctcgtcga aataatggtc
     1681 ctcctgcgag cgcatgacct cgagctggcg ccgccgctcg gcggttgcga accattcggg
     1741 ctcggcctgc tcgaggccat ggtcgcgcga gaactggtcg gcgccttcgc ggctcaggaa
     1801 gacgtggggg ctatgctcca tcaccgcccg ggccaggctg accggattgc gagtcgcggt
     1861 cactccggtc accgcgccgg cgtcgcggtc gcgcccgtcc atcaccgcgg catcgagctc
     1921 gttcgtgccc tgataggtga agaccgagcc ccggccggcg ttgaaattgg gatcgtcctc
     1981 gagcacgcag gccgcccgct cgacggcgtc gagggcgctg ccgccggccg ccaggatctc
     2041 cgacccggcg gcgagggcgc ggtcgagcgc ctcgcggatc tcacggtcct gctcgggcgt
     2101 cgaccgttcc cgctccagca ctccggcgcc gccgtggatg acgagcttcc aggggacttg
     2161 ggtcattcga aggctccgtt cggacagcga acgcacggag gggccgacct atccgtcgcc
     2221 aagggatcgg acaaggccgc ctttcccgtc attgcgagga gcgaagcgac gaagcaatcc
     2281 agcctcgcgc catcgtgccg ctggattgct tcgcttcgct cccgcaatga cgaaggggct
     2341 tgcgcccctc attgcatata attatgatat cataattaca ggagggcgaa atggcgactt
     2401 cacggcacga atctcccgac caccaggcct tgcgcctctc gcgcgagcgg ccggcccata
     2461 cctataacgg ctatgccatg ctgctggcgc tgctcgccgc gctcgtcgcg atggtcctcg
     2521 gcttcggcct ggcggtccgg gacggaggag gcgcagggcc ggtgctgctc gtcctcgccg
     2581 ccctcgtcgc gctcctgatc ctgttcggct tctacctgct ccagcccaac caggcggcgg
     2641 cgctgctgct gttcggcgac tataagggca ccgatcgcca ttcgggcctg cgctggcgct
     2701 ggccgtggct gacccgaacc aagatctcca cccgagtcca caacgtcacc tcggagcggc
     2761 tcaaggtcaa cgacctcagg ggcaacccga tcgagatcgc caccaacgtc gtctggcggg
     2821 tgaccgacac cgcccaggcg ctgttcgacg tcgacgacta caacaagttc gtcttcgtcc
     2881 agatcgagag cgcggtccgg gccatcgccg cccgctaccc ctatgacgat atcgagcatc
     2941 aggagacgac ccttcgcggc catgccgaga cggtcagcga cgagctcaag gaggagcttc
     3001 aggagcgggt cggggtcgcc ggcatcacca tcgacgaatg ccgcctcact catctcgcct
     3061 acgccgccga gatcgcccag tcgatgcttc ggcgccagca ggcggaagcg gtgatagccg
     3121 cgcggcagaa gatcgtcatc ggcgcggttt ccatggtcga gaacgcgctc cagctcctgt
     3181 cggaaaaagg cgtcgtcgag ctggacgacg aacgca
//
LOCUS       DASVQZ010000098         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_214667, whole genome shotgun sequence.
ACCESSION   DASVQZ010000098 DASVQZ010000000
VERSION     DASVQZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214667"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VF547_02660"
     CDS             <1..350
                     /locus_tag="VF547_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8261755.1"
                     /translation="APLRMASHIGLWLVAASVLLLAYIAVGFFTGTAIQGWTSLMLVV
                     VVLGAVQMFVLGMIGEYLGRLYMEAKHRPLYIVAEIAGHAGGSPSLGHVAHEAETIAT
                     SDTPGGNGSRPIA"
     gene            complement(284..1012)
                     /locus_tag="VF547_02665"
     CDS             complement(284..1012)
                     /locus_tag="VF547_02665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8261756.1"
                     /translation="MERIVYDRMAELDSRHWWYRARREILADLIERRIDLPEDPRILE
                     IGCGTGHNLVMLKRFGRVDAIEIDPAARDIASRRLGHPVMDAPLPALTGVEDRAYDLV
                     AILDVLEHVDEDREALESIARKLRPGGRILIAVPAHPWMWSAHDVVNHHKRRYTRKTL
                     RAVVAAAGLKLEMLSWFNSLLFPLAAAARFAGKVRRKEDSDDKLPPRPVNALFEAIFG
                     LERYAIGRLPFPPGVSLVAIVSAS"
     gene            complement(1013..1417)
                     /locus_tag="VF547_02670"
     CDS             complement(1013..1417)
                     /locus_tag="VF547_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8261757.1"
                     /translation="MKPIGAIPPLFGQVIRFGLVGGFVTVIGMAVYYFPATYMGVAPL
                     LANLLAYAVAMALGYVLHSSVSFRGHGSRDNPARRTGRFLVVSLVSLGLNSLWVWTLT
                     GLLDGPTWWPLVPMLFVTPAATFALNRQWVFG"
     gene            1503..>1997
                     /locus_tag="VF547_02675"
     CDS             1503..>1997
                     /locus_tag="VF547_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HEX8261758.1"
                     /translation="MEGLDGLERRWRWFVLLFWLAASGLLLYTRWAQINAFALGDTDD
                     NMRMMQVRGLLAGQDWYDLRQHRLNPPAGADIHWSRLADLPIAGIRLALAPFTGGAVA
                     EKAAVALAPLLPMAAAMAAVAVTARRLVAPRAFALAIAVLLCGHSARAMWSPLRIDHH
                     GWQLA"
BASE COUNT          281 a          679 c          723 g          314 t
ORIGIN      
        1 cggcgccgct caggatggcg agccatatcg gcctttggct ggtcgccgcc tcggtgctcc
       61 tgctcgccta catcgccgtc ggcttcttca ccggcaccgc gatccagggc tggacctcgc
      121 tgatgctggt cgtcgtcgtg ctcggcgcgg tgcagatgtt cgtgctcggg atgatcgggg
      181 aatatctcgg ccggctctac atggaggcca agcaccggcc gctctacatc gtcgccgaga
      241 tcgccggcca cgccggcggc agcccgtcgc taggccatgt cgctcacgaa gcggagacga
      301 tcgcgaccag cgacacgccc ggcgggaacg gcagccgccc gatcgcgtag cgctcgaggc
      361 cgaatatcgc ctcgaacagc gcgttgaccg gcctgggcgg aagcttgtcg tcgctgtcct
      421 ccttcctgcg caccttgccg gcgaagcggg cggcggccgc gagcggaaag agcaggctgt
      481 tgaaccagct gagcatctcg agcttcagcc cagcggcggc gaccacggcg cgcagggtct
      541 tcctcgtgta gcggcgcttg tgatggttga cgacgtcgtg ggcgctccac atccagggat
      601 gggccggcac ggcgatcagg atgcggccgc cgggcctcag cttccgggcg atgctctcga
      661 gcgcttcccg gtcctcgtcg acatgctcga gcacgtcgag gatcgcgacc agatcatagg
      721 ccctgtcctc cacgccggtc agcgcgggca ggggggcgtc catcacgggg tggccgagcc
      781 gccggctggc gatgtcgcgg gcggcggggt cgatctcgat cgcgtcgacc cggccgaagc
      841 gcttgagcat gaccaggttg tggccggtgc cgcagccgat ctcgaggatt cgcgggtcct
      901 cgggcaggtc gatccggcgc tcgatcaggt cggcgaggat ctcgcggcgc gcgcggtacc
      961 accagtgccg gctgtcgagc tccgccatgc ggtcgtagac gatcctctcc actcagccga
     1021 acacccattg gcggttgagc gcgaaggtgg cggccggagt gacgaacagc atcggcacga
     1081 gcggccacca ggtcggcccg tcgagcagcc cggtcagggt ccagacccag aggctgttga
     1141 gcccgaggct gaccagcgag acgaccagga agcgcccggt ccgcctcgcg gggttgtcgc
     1201 ggctgccatg gcccctgaag ctgacgctgc tgtggaggac atagcccagc gccatcgcga
     1261 cggcgtaggc gagcaggttc gcgagcagcg gcgccacgcc catgtaggtg gccgggaaat
     1321 aatagacggc catcccgatc acggtgacga agccgccgac gaggccgaag cggatgacct
     1381 ggccgaacag gggcgggatc gcgccgatcg gcttcatctc agcgcgtttc cttcctgctc
     1441 acggcttgcc ctttgtcgtg cagcgactaa ggccgtggcg aggggttgaa aagggtcttc
     1501 ggatggaggg gctggacggg ctggagcgac gctggcgctg gttcgtcctg ctcttctggc
     1561 tcgcggcctc gggcctcctc ctctacacgc gctgggccca gatcaacgcg ttcgcgctcg
     1621 gcgacaccga cgacaacatg cggatgatgc aggtccgcgg cctcctcgcc ggccaggatt
     1681 ggtacgatct ccgccagcac cggctgaacc cgccggcggg cgccgacatc cactggtcgc
     1741 ggctggccga cctgccgatc gccggcatcc ggctggcgct ggcgccgttc accggcggcg
     1801 cggtcgcgga gaaggcggcg gtggcgctgg ccccgctgct gccgatggcg gccgcgatgg
     1861 cggcggtcgc ggtcacggcg cggcggctgg tggcgccgcg ggccttcgcg ctggcgatcg
     1921 ccgtcctgct ctgcggccat tcggcccgcg ccatgtggtc gccgctcagg atcgaccatc
     1981 acggctggca gctggcg
//
LOCUS       DASVQZ010000099         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_20869, whole genome shotgun sequence.
ACCESSION   DASVQZ010000099 DASVQZ010000000
VERSION     DASVQZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20869"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /gene="rpmG"
                     /locus_tag="VF547_02680"
     CDS             complement(<1..158)
                     /gene="rpmG"
                     /locus_tag="VF547_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX8261759.1"
                     /translation="MAKPTTVKIKLVSTADTGFYYVTKKNPRNITEKMTFRKYDPKVR
                     KHVEFKEA"
     gene            515..718
                     /locus_tag="VF547_02685"
     CDS             515..718
                     /locus_tag="VF547_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261760.1"
                     /translation="MRQLAAAYALTPVVRVAEALERAVAEDRRACPRALYLGRLQDAI
                     GCETMEESAAEAMLASVSIRLCA"
     gene            722..1288
                     /locus_tag="VF547_02690"
     CDS             722..1288
                     /locus_tag="VF547_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX8261761.1"
                     /translation="MEALLISFIAAALGEWGDKTQLLVIALAARYRRPLPILAGVLAA
                     ALANSLIAAFGGALVHDMVAPRAASLLVALALIFAGVAGLIRPKPYESAGTSRGGPFL
                     VAAASFFVLEFGDKTQFLTFAIAARFDAFALAAAGATAGIMAGSVPAALLGQGLAKAV
                     PLKALRIGFGALFLAVGLVVGLSARRLL"
     gene            complement(1285..2112)
                     /locus_tag="VF547_02695"
     CDS             complement(1285..2112)
                     /locus_tag="VF547_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8261762.1"
                     /translation="MRRTLQRLFVMLLLAATAVVAWAYWTAVSDPVVRSTRVAAARWP
                     GNVPPLRLLLMSDVHVGGPDMPPARLRRIVEQINRLRPDIVLIAGDLVTDKRLATRYY
                     SHDEAVAPLAGLRPRLGTVAVLGNHDHWRDAAAARRALAEANIRVLANQAVQVGPVAV
                     GGLDDDFTGRADLRATLAALRRLDGPRLILSHSPDPFPDAAPDVFLMLAGHTHCGQVA
                     PPLIGPLSTMSKYGRRYACGVVRERGRTLVVTAGLGTSGIPLRLGAVPDLWLVEVGP"
     gene            complement(2117..2398)
                     /locus_tag="VF547_02700"
     CDS             complement(2117..2398)
                     /locus_tag="VF547_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8261763.1"
                     /translation="MSGEAPPCFLCGRTLGRRVEWHHPVPRSLGGRETVPVHPICHRA
                     IHRALDNKALARDYASPAALRAHPELARFIAWVSGKDPDFHAPTRRGRK"
BASE COUNT          362 a          880 c          857 g          385 t
ORIGIN      
        1 ttcgcttcct tgaactcgac gtgcttgcgc accttcggat cgtatttgcg gaaggtcatc
       61 ttctcggtga tgttccgagg attcttcttg gtcacgtaat agaagccggt gtcggcggtg
      121 ctgacgagct tgatcttgac cgtggtcggc ttggccatga cagcctctcg aattcggggt
      181 ttcgtaaaaa ataaaagcgg cgtccccgcc gctcgaatgc gccgccgcct ctgccccgaa
      241 gcagtgccgc tgtcaagggt tgcgggcctt cgaagccgcg cgaggtccga tcgccgcgga
      301 ccgaccggag attcgaagcc tgacgctcgc cgctttaacg aagcggtaac cctatcggcg
      361 catcgtcgcc ccgaccgacc aggggagcgg gacgatgcgg gccgaagcgg atggaatgat
      421 gctcgttcga gcggagcttt gcgagcgcct cgccagcctc cagacggcgg caaggcgcct
      481 ttcccaccgt gatttcgtgg cgagcgtcgg cgccatgagg cagctggccg ccgcttacgc
      541 gctgacgccg gtggtgcggg tagccgaagc cctcgaaaga gcggtcgcgg aggaccgccg
      601 cgcctgtcct cgcgccctct atctcggccg actccaggat gcgatcggct gcgagacgat
      661 ggaggagagc gccgcggaag cgatgctcgc ctcggtctcg atccgcctct gcgcctgagc
      721 cttggaagcg ctcctcatca gcttcatcgc cgccgcgctc ggcgagtggg gcgacaagac
      781 ccagctgctc gtcatcgccc tcgccgcccg ctaccgcagg cccctgccga tcctcgccgg
      841 ggtgctggcc gcggccctcg ccaacagcct gatcgcggcc ttcggcggag ccctcgtcca
      901 cgacatggtg gcccccaggg cggcctcgct tctggtcgcg ctcgcgctga tcttcgccgg
      961 cgtggccggg ctgatccggc ccaagcccta cgagtcggcc ggcacttccc ggggcggtcc
     1021 cttcctcgtc gccgccgcct ccttcttcgt gctcgaattc ggcgacaaga cccagttcct
     1081 gaccttcgcg atcgccgccc ggttcgatgc cttcgcgctg gccgccgccg gggcgacggc
     1141 cggaatcatg gccggaagcg tgcccgccgc cctgctcggg cagggcctcg ccaaggcggt
     1201 cccgctcaag gccctgagga tcggcttcgg agccctgttc ctggccgtcg gcctcgtcgt
     1261 cgggctgagc gcccggcgcc tcctctaagg gccgacctcg accagccaga ggtcgggcac
     1321 cgctcccagg cgcagcggta ttccgctggt gccgagcccg gccgtcacca ccagcgtcct
     1381 cccgcgctcg cggaccaccc cgcaggcgta gcggcggcca tatttcgaca tggtggaaag
     1441 cgggccgatg agaggtgggg caacctgccc gcaatgggta tggccggcga gcatcaggaa
     1501 cacgtccggc gcggcgtccg ggaacggatc gggactgtgg ctgaggatga gcctcggccc
     1561 gtccagccgg cgcaaggcag cgagggtcgc gcgcagatcg gccctgccgg tgaaatcgtc
     1621 gtccaggccg ccgaccgcca cgggtccgac ctgcacggcc tgattggcca gcacgcgtat
     1681 gttcgcctcg gcgagggccc ggcgggcggc ggcggcatcg cgccaatgat cgtgattgcc
     1741 gagaacggcg acggtcccaa gccgagggcg gagcccggca aggggcgcga ccgcttcgtc
     1801 gtgggaatag tagcgggtgg ccaggcgctt gtcggtcacg aggtccccgg cgatcaggac
     1861 gatgtcgggc cggagccggt tgatctgctc cacgatccgg cgcagccgcg ccggcggcat
     1921 gtccggtccg ccgacatgca cgtccgacat gaggagcagc cgaagcggag gaacattgcc
     1981 cggccagcgg gcggcggcga cccgggtgct ccggacgacc ggatcggaca ccgccgtcca
     2041 ataggcccat gcgacgaccg ccgtcgcggc gagcaggagc atcacaaaga ggcgttggag
     2101 cgtgcgccgc atcgtctcac tttcggcccc gccgcgtcgg ggcgtgaaaa tccggatcct
     2161 tgccgctgac ccaggcgatg aagcgcgcca gctcgggatg cgcgcgaagc gccgccggcg
     2221 aagcatagtc ccgcgccagc gccttgttgt cgagcgcccg gtggatggcg cggtggcaga
     2281 tcggatggac cggcaccgtc tcgcgtccgc cgaggctgcg cggcaccgga tgatgccact
     2341 cgacccgccg gcccagcgtc cggccgcaga ggaagcacgg cggcgcctcc ccgctcacgc
     2401 ggcgaccgtc tccgccccgg gatagtccgt atagccttcc ggcccctgac tgtaccaggt
     2461 ggccatcacg tccggagcca gctc
//
LOCUS       DASVQZ010000100        12075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_499503, whole genome shotgun sequence.
ACCESSION   DASVQZ010000100 DASVQZ010000000
VERSION     DASVQZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499503"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF547_02705"
     CDS             <1..199
                     /locus_tag="VF547_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261764.1"
                     /translation="CASSGSFVETGVAAIAVRPGPTVNPPDRRGLAEKVNDLGAEGNR
                     LFPKPFSAVRKRTMLQNGYTR"
     gene            375..3773
                     /locus_tag="VF547_02710"
     CDS             375..3773
                     /locus_tag="VF547_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8261765.1"
                     /translation="MAFAAPALAQGTTAPQDADPAELQSETEVQSGVDAQTGAQGEGA
                     TAGGDQTITVTGSRIRRPNLESTVPITSIGGEQFFQTGDTNIGDTLNDLPQLRSTFAQ
                     QNPGLGIGIAGLNLLDLRGLGTSRTLVLVNGRRHVPADILNNAVSPDINTIPNDLIER
                     VDIVTGSQSATYGSDAIAGVVNFILRRNFDGIQVRGQAGIADGGFGGNQYVSAMVGRN
                     FMGDRANITLHGEYAKQDRVFASHIDHLRTQNGFLVVDVDTAGLPNGSDNFPDRTFFR
                     DIRSASINRFGLVPITQAGTTTAVAGGTLPAGTVITATPQACGQGIGSTNGGPGPGSG
                     ASVGTPYNCTFLFTPTSNLIPQTGTRTGQGIIGGIVGGNGQTGREGQLLSVFPFVERY
                     NFNLLAHFSVSDAFEPFLEAKWNRVNALGNNAGPSFIQGTFGQFDTRERVRLDNPFLS
                     PADRTAIANAILASGCNTSLTAPCAVPGVASSFNRTTRADPTLPGSPVQGIGGPLNAA
                     DRAAIAAGTYRFVLARQLADAGIRDEQFQRDTYRIVGGVRGTFNEDWNYELAVNYGKF
                     KQDVTTFGFLDRQRFLLSLDAGRNPATGQIQCRSQFDPAARVPFAGSDARLAADVAAC
                     VPYNPFGGSDNSAAVDYFSVNAKTKAGLEQFDVLGYVGGDTSQIFELPGGPISFVLGG
                     EYRRERARYEDDDFVESGATNAVIIGEFDPPTFKVKEAFAEIRIPILRDMPFFHELTV
                     TGAGRVSDYNSAVGTVYTYNVGGDWAPVRDLRFRAGYGKAVRAPNVSETGFPAVPNFA
                     PGFLDPCASGQIANNPNRQANCQEALGALLPSLPNVAYSLPIISGSNPNLAEETSKSL
                     TVGGVFQPRFVPGFSLSVDYYDIKVSNVIVSLSAQAIANGCYDQPSLQNPLCGVFERF
                     MGPGTSPGGFAPGQILENSLVSAPVNFAKLVRRGIDVNAAYRTRFGKDVRFDANLIYV
                     HNLQTSNFLNPSDPNFENRILGEVGDPKDEFRLDADLRYKAFTFGYRLRYIGPAAFSA
                     FEDANPLDSACSAAGCPPNNADFADRDSVPAVWYHDIRFAWDLDNLGGLGKSFQFYFG
                     VDNLLNRKPPLGIAATGTGPGGVGNGAIYDIRGRNFYGGFRARF"
     gene            complement(3868..4512)
                     /locus_tag="VF547_02715"
     CDS             complement(3868..4512)
                     /locus_tag="VF547_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261766.1"
                     /translation="MKVLGRYKKDGYAHLKGLVPVEVAQAFLQRLKADIGPAPIPLSR
                     APSHVNLLTRPAFEAYGHHYTPMLYFLWGLTPIMEQIVGRALLPTYDYLRIYRQGDVC
                     KVHSDRYSCEHSLSLTLGYSDGKPWALEVETARSEPSSKVDEAFGARFSSVAMQVGDA
                     VLYQGVHHRHGRTVPNPNRWSAHLFLHWVDREGPYREHAFDGQMKPAPVDFSFS"
     gene            4619..5608
                     /locus_tag="VF547_02720"
     CDS             4619..5608
                     /locus_tag="VF547_02720"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8261767.1"
                     /translation="MAADDMMVRIDALVGGGRHAEAARLLMAAAAGGDPAALSQLGFW
                     RVAGSIVRRDLAKARALFAEAAAKGDSGAALIHASFLANGTGGEAQWPEALAALRRLA
                     GKVPQAAAQLRLIETMDLDEDGFPARPIPVRPLAASPHAVAAEGFLTRAECAYLIRAA
                     ETGFQPSVVVDPATGRMVPHPGRICDAAMFGVFAEDLAVNAINRRIAALSGTAPAQGE
                     SLQVLRYRPGGQYKPHMDGLPAEPNQRILTVLVYLSDDYEGGETRFERTGLAFRGKAG
                     DALLFRNANAEGRPDPMSLHAGLPVAKGVKYLASRWIRARAFTFPPPRPLLDL"
     gene            complement(5571..6497)
                     /locus_tag="VF547_02725"
     CDS             complement(5571..6497)
                     /locus_tag="VF547_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8261768.1"
                     /translation="MARIAHLSDIHFGRNDPKVVDGVNAWLEAQQPDLVIISGDFTQR
                     ARIEQFREASAWLNRLRASGWPILAVPGNHDVPLYDVFTRFAAPLRRYKRYISNDLCP
                     WFEDDEVAVLGINTARSLTIKDGRINREQMKIIEDRFAAVAPDKTKILVTHHPLFSMP
                     IGEGTELTEAVGRHEDAVAAVCRAGVNIALAGHFHRTYAEAARKMVETAGGALVIQAG
                     TATSTRLRNNELQSFNWIHAHRNNDIELQVIAWDGSGFRRGSHVRFAYDGENWSSRTI
                     SHDDPLETAAAGLAQVPEPAAKDPEGAEAAGR"
     gene            complement(6599..7645)
                     /gene="alr"
                     /locus_tag="VF547_02730"
     CDS             complement(6599..7645)
                     /gene="alr"
                     /locus_tag="VF547_02730"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8261769.1"
                     /translation="MPQPHSPLRLLLDGDALVANWRWLAGQGAGAACGAAVKADGYGL
                     GARDVARRLANEGCRDFFVATWPEAEALLPWLGAASVSVLHGLAEEDLAHAAGSPARP
                     VLNTALQVQRWKQAAPGRPCDVMIDTGINRLGLSPAEVAAGLLGGLEIDTLMSHLACA
                     DEDVAMNERQRAAFAEIAACVPAKRRSLANSAGICLGPAYAFDLTRPGLALYGGVPRA
                     EAAGHIRQVVRVEAQIVQRRRVEAGASVGYNAAWTAHAPTELAILNLGYADGYLRGFS
                     GTGRARVGGGYAPVIGRVSMDLTALRVDEAPELKEGDWVELDYALPAAAAQSGLSQYE
                     LLTTLGSRHERVWT"
     gene            complement(7746..9341)
                     /locus_tag="VF547_02735"
     CDS             complement(7746..9341)
                     /locus_tag="VF547_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261770.1"
                     /translation="MATAVSGDYVIDAKRDRLVIFASTLGTVFEWYDFFIYGTLAAVI
                     GKQFFPASSETAQFLLALASFGVGFGVRPIGAVLFGYLGDKLGRKYTFLVTITLMGIA
                     TAAVGLLPGYAAIGVAAPALLVFLRVLQGLALGGEYGGAAIYVAEHAPRNRRGFYTSF
                     IQAGVIGGFILSVAVVLASELFVDKAQWEAWGWRIPFLFSIVLLAVSLWVRLKLKESP
                     VFQAMKEAGEVAKNPLAETFATWSNIKLVLVALFGIAAGLTVIWYTAQFQAMYFLQNS
                     LRIEDTAARAMIGAAAVISMIWFILFGWLSDRVGRKGPIVIGYALTVVLIFPLFHWMA
                     SAANPELSAAMRKNPVVVTGSDCSYAPFAKVQPTDCGKLLDALSKKGIAYSTADAPAG
                     APPQVTIGGVAVDASDPASLDFGLKIAGYKLEKSAPTFGQALQVVLAIVLIGLCSGMT
                     YGPVAALLVELFPARIRYTSMSIPYHIGTGYFGGFLPLISQYIVARTGDPFAGLWYTV
                     GVVAMALVVTFLWLPETAGKELE"
     gene            complement(9542..10156)
                     /locus_tag="VF547_02740"
     CDS             complement(9542..10156)
                     /locus_tag="VF547_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8261771.1"
                     /translation="MADDLNSVKSGRGYHHGALRPALIAAAEAVLAERGTQGFSLREA
                     ARRAGVSPAAPAHHFGDASGLLTAVAAGAFRDLGDALEAALAADGRAARIRAQGSAYV
                     RFALANRARFDLMFRAALLKRDDPDYAAAAARAFRLLDDAVRGGEGPAPRPGAPGTAP
                     SIACWSTVHGFARLAIDGEFGTLPGAAEAAAEVLLPAVLDHLAV"
     gene            10192..10641
                     /locus_tag="VF547_02745"
     CDS             10192..10641
                     /locus_tag="VF547_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HEX8261772.1"
                     /translation="MLDPNIAFGFANNFGLVAWLALLASLFVPRLRSWVWPATQFVIP
                     AVWAAAYVLLLVQGMPETDGSFSSIEGVRSLFGSDSALTAGWLHYLAFDLFVGTWIAR
                     DSTERRIHGLLVVPCLALTLVFGPSGLLLYLLMRLAFGRRDPTETAS"
     gene            10638..11462
                     /locus_tag="VF547_02750"
     CDS             10638..11462
                     /locus_tag="VF547_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261773.1"
                     /translation="MTALGLPLPLTRLPILTELLERQRLLALYGLATLLLALPVLALQ
                     AIDPRTLHGVGVWVKPAKFLVSVGIFALTAAWYWGYVAPERRGSGPLRLTAAVLVGSA
                     SFELLYILLQAGRGLDSHFNFTSPLYVAMYGLMGLAAVLLVGTVLPLAWQIVRHPVPG
                     LRGDFVAALAIGLVLTFLLGGALGGYMSAQAGHSVGAQGGHVPFFGWNRSGGDLRVAH
                     FLGIHAEQALPLFAASMAGRSPRMRWTGLALATLAYVALTLAVFAQAVAGKPLLAL"
     gene            complement(11472..11852)
                     /locus_tag="VF547_02755"
     CDS             complement(11472..11852)
                     /locus_tag="VF547_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8261774.1"
                     /translation="MNPVPISGGCHCRAVRFEARVPAAVEVLDCNCSICAMTGFRHLI
                     VPHSDFRLVSGEAKLTHYRFGTGAADHLFCAVCGVKSFYQPRSHPEAWSLNLNALDDV
                     SALEVTVRSFDGRNWEAARSGLAG"
     gene            complement(11863..>12075)
                     /locus_tag="VF547_02760"
     CDS             complement(11863..>12075)
                     /locus_tag="VF547_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261775.1"
                     /translation="SVRAAFSRLQKEGYAVAGGEAAAPRPPRPGGPPASQHRPFRRPP
                     PKRDGAGPGRPPGKGGGRPPRKPPSR"
BASE COUNT         1867 a         4262 c         4080 g         1866 t
ORIGIN      
        1 ctgtgcgtcg agcggtagct tcgtcgagac cggcgtcgcc gcgatcgccg tccgccccgg
       61 cccgacggtg aatccgccgg atcgccgggg attggccgag aaagtgaatg acctcggcgc
      121 cgaaggcaac cggctcttcc caaagccttt ttccgccgtc cgcaagcgga cgatgttgca
      181 gaacggttac actcgctgaa cttttgatga accgaccgcc tcccgccctt gcgcgggggg
      241 gatgaatcgt ctctgcgtta cctcagaggc cgtgtgcagc aagcagggcc gtcaaagagg
      301 gatgaatcgg gatgagaaag tttggcctcc tcggcacgag cgcgattcgc tctgccgcct
      361 ttatcggctt ttcgatggcg ttcgccgcgc cggccctggc gcagggcacc actgcccccc
      421 aggatgcgga tccggccgag ctgcagagcg agactgaggt tcaatcggga gtcgacgcgc
      481 agaccggcgc gcagggcgaa ggcgccaccg ccggcggcga ccagacgatc accgtcaccg
      541 gctcgcgcat ccgcaggccg aacctcgaat cgacggttcc gatcacttcg atcggcggcg
      601 agcagttctt ccagacgggc gacaccaaca tcggtgacac cctgaacgac ctgccgcagc
      661 tccgcagcac tttcgcccag cagaatccgg gcctcggcat cggcatcgcc ggcctcaacc
      721 tgctcgacct tcgcggtctc ggcacgtcgc gcacgctggt gctggtcaac ggccgccgcc
      781 acgtccccgc cgacatcctc aacaacgccg tgtcgccgga catcaacacg attccgaacg
      841 acctgatcga gcgcgtcgac atcgtcaccg gcagccagtc ggcgacctac ggctcggacg
      901 ccatcgccgg cgtcgtcaac ttcatccttc gccgcaactt cgacggcatc caggtgcgcg
      961 gccaggccgg catcgcggac ggcggcttcg gcggcaacca atatgtctcg gcgatggtcg
     1021 gccgcaattt catgggcgac cgcgccaaca tcaccctcca tggcgaatat gcgaagcagg
     1081 atcgcgtgtt cgcgtcgcac atcgaccacc tgaggaccca gaacggcttc ctcgtcgtcg
     1141 acgtcgacac ggccggactg ccgaacggca gcgacaactt cccggatcgc accttcttcc
     1201 gcgacatccg cagcgccagc atcaaccgct tcggcctggt cccgatcacc caggcgggaa
     1261 cgaccacggc cgtggccggc ggcacgcttc cggcgggtac cgtcatcacg gccactccgc
     1321 aggcttgcgg ccagggcatc ggttcgacca acggcggccc cgggccgggc tcgggcgcgt
     1381 ccgtcggcac gccgtacaac tgcaccttcc tcttcacccc gacgagcaat ctcatcccgc
     1441 agaccggtac ccgcacgggc cagggcatca tcggcggcat cgtcggcggc aatggccaga
     1501 ccggccgtga ggggcagctg ctttcggtgt tcccgttcgt cgagcgctac aacttcaacc
     1561 tgctcgcgca cttctcggtc agcgacgcct tcgagccgtt cctcgaagcc aagtggaacc
     1621 gggtcaacgc gctcggcaac aatgccggtc cgtcgttcat ccagggcacc ttcgggcagt
     1681 tcgacacccg cgagcgcgtc cggctggaca acccgttcct cagccccgct gaccggacgg
     1741 cgatcgccaa tgccatcctg gcctcgggct gcaacaccag cctcaccgcg ccgtgcgccg
     1801 tgccgggcgt tgcgagcagc ttcaaccgga cgacccgcgc cgacccgacg cttccgggat
     1861 ccccggtcca gggcatcggc ggtccgctga atgccgcgga ccgcgccgcg atcgccgccg
     1921 ggacctatcg tttcgtgctt gcccggcagc tggccgacgc gggcatccgc gacgagcagt
     1981 tccagcgcga cacctaccgc atcgtcggcg gtgtccgcgg cacgttcaac gaggactgga
     2041 actacgagct cgccgtcaat tacggcaagt tcaagcagga cgtgacgacg ttcggcttcc
     2101 tcgaccgcca gcggttcctg ctgtcgctcg atgccggccg caacccggcc acgggccaga
     2161 tccagtgccg ttcgcagttc gatccggccg cccgcgtgcc cttcgcgggc agcgacgccc
     2221 ggctcgccgc cgacgtcgcc gcctgcgttc cctacaatcc gttcggcggc tcggacaaca
     2281 gcgctgcggt cgactacttc agcgtcaacg cgaagaccaa ggcggggctc gagcaattcg
     2341 acgtgctcgg ctatgtcggc ggcgacacga gccagatctt cgagctgccc ggcggcccga
     2401 tcagcttcgt tctcggcggc gagtatcgcc gggagcgtgc gcgctatgaa gacgacgact
     2461 tcgtcgagag cggcgcgacc aacgccgtca tcatcggcga gttcgatccg ccgaccttca
     2521 aggtgaagga ggccttcgcc gagatccgca tcccgatcct gcgcgacatg cccttcttcc
     2581 acgagctgac ggtgacgggc gccggccgcg tgtccgacta caattcggcg gtcggaacgg
     2641 tctacaccta caatgtcggc ggcgactggg cgccggtccg tgaccttcgc ttccgcgccg
     2701 gctacggtaa ggcggtgcgc gcaccgaacg tttcggagac cggcttcccg gccgttccga
     2761 acttcgctcc ggggttcctg gatccgtgcg cttcgggcca gatcgccaac aaccccaacc
     2821 gtcaggcgaa ctgccaggag gctctcgggg cgctgctccc gagccttccc aacgtcgcct
     2881 attcgctgcc gatcatcagc ggcagcaacc cgaacctggc cgaggaaacg tcgaagtcgc
     2941 tcactgtggg cggagtgttc cagccgcgct tcgtgcccgg cttctccctc agcgtcgatt
     3001 attacgacat caaggtcagc aacgtcatcg tctcgctgtc cgcccaggcg atcgcgaacg
     3061 gatgctacga ccagccgagc ctgcagaacc cgctctgcgg ggtcttcgag cgcttcatgg
     3121 gtccggggac cagcccgggc ggcttcgcgc ccggccagat cctcgagaac tcgctcgtct
     3181 cggcgccggt caacttcgcc aagctggtgc gtcgcggcat cgacgtgaac gcggcctatc
     3241 gcacgcggtt cggcaaggac gtccgcttcg acgccaatct gatctatgtg cacaacctcc
     3301 agacgagcaa cttcctgaac ccgagcgacc cgaacttcga gaatcgcatt ctcggcgagg
     3361 tcggcgatcc gaaggacgag ttccggctgg atgcggacct caggtacaaa gccttcacgt
     3421 tcggttaccg gctccgctac atcggtccgg cggcgttcag cgcgttcgag gatgccaacc
     3481 cgctggatag cgcctgcagc gccgccggct gcccgcccaa caacgcggac ttcgcggaca
     3541 gggacagcgt cccggccgtc tggtatcacg acatccgctt cgcctgggat ctcgacaacc
     3601 tcggtggtct tggaaagagc ttccagttct acttcggcgt cgacaacctg ctcaaccgca
     3661 agcccccgct gggcatcgcg gcgacgggca ccggcccggg cggcgtcggc aacggcgcga
     3721 tctacgatat ccgcggtcgc aacttctacg gcggcttccg cgcccgcttc taatagtcct
     3781 tccaagatct cgggggccgg aacgatgttc cggccccctt tttttgtcga tcgtggacgg
     3841 cgcgcgggcg cttcgccgcc gtgctgctca gctgaagctg aagtcgaccg gagccggttt
     3901 catctggccg tcgaacgcat gctcgcgata cggcccctcg cggtccaccc agtgcaggaa
     3961 gaggtgcgcg gaccagcggt tgggattggg cacggtgcgg ccgtggcgat ggtggacgcc
     4021 ctgatagagg acggcgtcgc cgacctgcat ggcgacggac gagaagcggg cgccgaaagc
     4081 ctcgtcgacc ttcgacgagg gctcgctgcg cgccgtctcg acctcgagcg cccaaggctt
     4141 gccgtcgctg tagccgagcg tcagcgacag gctgtgctcg cacgaatagc ggtcggaatg
     4201 gaccttgcag acgtcgccct gccggtagat gcgaagataa tcgtaggtgg gaagcagggc
     4261 gcggccgacg atctgctcca tgatcggagt gagcccccac aggaaataga gcatcggcgt
     4321 gtaatgatgg ccataggcct cgaaagccgg ccgggtcagc aggttgacgt ggctcggggc
     4381 gcggctgagc gggatcgggg ccggtccgat atcggccttc aggcgctgca ggaaggcctg
     4441 agcgacctcg accggcacca ggcccttgag atgggcgtag ccgtcctttt tgtagcggcc
     4501 gaggaccttc atcgggccag cgctaatgcg aggacggttg gaccgcaagc ggcccgggcc
     4561 gcggcggccg agtcgcggcc caccttttct tatcgcccgc aggcactaag gaaaaggcat
     4621 ggccgctgac gacatgatgg tccgcatcga cgcgctcgtg ggcggcggcc ggcacgccga
     4681 ggcggcgcgc ctgctgatgg ccgccgcggc cggcggcgat ccggcggcgc tctcccagct
     4741 cggtttctgg cgggtcgccg gaagcatcgt gcgccgcgat ctggcgaagg cccgcgccct
     4801 gttcgccgag gcggcggcga aaggcgattc cggcgcggcc ctgatccacg cctcgttcct
     4861 ggccaacggc accggcggcg aggcgcaatg gccggaggcg ctggcggccc tgaggaggct
     4921 cgccggcaag gtcccgcaag cggccgcgca gctgcggctg atcgagacga tggacctcga
     4981 cgaggacggt tttcccgctc ggcccatccc ggttcgtccg ctcgcggcct cccctcatgc
     5041 ggtcgcggcc gaaggcttcc taacccgggc ggaatgcgcc tatctgatcc gagccgccga
     5101 gaccggcttc cagccttcgg tggtggtcga tcccgccacc ggccggatgg tgccgcaccc
     5161 gggccggatc tgcgacgcgg cgatgttcgg agtcttcgcc gaggatctcg cggtcaacgc
     5221 gatcaaccgc cggatcgccg ccttgagcgg caccgctccc gcccagggcg agtcgcttca
     5281 ggtcctgcgt taccggcccg gcggccagta caagccgcac atggacggcc ttcccgccga
     5341 gccgaaccag cgcatcctca cggtcctcgt ctacctctcc gacgattacg agggcggcga
     5401 gacgcggttc gagcgcaccg gccttgcctt ccgcggcaag gcgggcgacg cgctcctgtt
     5461 ccgcaacgcc aacgccgagg gccgcccgga tccgatgtcg ctccacgccg gcctgccggt
     5521 cgcgaagggg gtcaaatatc tcgcctcgcg ctggatccgg gcccgggcct tcaccttccc
     5581 gccgcctcgg ccccttctgg atctttagcg gccggctcgg ggacttgcgc gagcccggcc
     5641 gccgccgtct cgagcggatc gtcgtgagag atcgtccgcg agctccaatt ctcgccgtca
     5701 taggcgaagc ggacgtggct tccgcggcgg aagccgctgc cgtcccaggc gatcacctgc
     5761 agctcgatgt cgttgttccg atgggcgtgg atccagttga agctctgaag ctcgttgttg
     5821 cgcaggcggg tcgaggtcgc cgtccccgcc tggatgacga gcgctccgcc ggcggtttcg
     5881 accatcttgc gcgcggcctc ggcataggtg cggtggaaat ggccggcgag ggcgatgttg
     5941 accccggccc ggcacaccgc cgccaccgcg tcctcgtggc ggccgacggc ctcggtaagc
     6001 tcggtcccct cgccgatcgg catcgagaag agcgggtggt gggtgacgag gatcttggtc
     6061 ttgtccgggg cgaccgcggc gaaccggtcc tcgatgatct tcatctgctc gcggttgatc
     6121 cggccgtcct tgatggtgag ggagcgggcg gtgttgatgc cgagcacggc cacctcgtcg
     6181 tcctcgaacc aggggcacag gtcgttggag atgtagcgct tgtagcggcg cagcggcgcg
     6241 gcgaagcggg tgaagacgtc gtagagcggg acgtcgtgat tgccggggac ggcgaggatc
     6301 ggccagcccg aggcgcgcag ccggttgagc caggccgagg cctcgcggaa ttgctcgatc
     6361 cgggcgcgct gggtgaagtc gcccgagatg atgacgaggt cgggctgctg cgcctcgagc
     6421 caggcgttga cgccgtcgac gaccttcgga tcgttgcggc cgaaatgaat gtcggagaga
     6481 tgggcgatcc gggccatggg gggagaactc gttcctttcg ccggagttgc ccaccgcgca
     6541 gcaactcttc cggaaaccac cctcccccgg cccctccccc gcgtgcaggg agggggggct
     6601 acgtccacac ccgctcgtgc cggctcccga gcgtcgtcag aagctcgtat tgcgacaggc
     6661 cggactgggc ggcggcggct gggagggcgt aatcgagctc gacccagtcg ccctccttca
     6721 gctccggcgc ctcgtcgacc cggagcgcgg tgaggtccat cgagacccgg ccgatcaccg
     6781 gggcgtagcc gccgccgacg cgggcccggc cggtgccgga aaagccgcgc agatagccgt
     6841 ccgcatagcc caggttgagg atcgcgagct cggtcggggc atgggcggtc caggcggcgt
     6901 tgtagccgac gctggcgccc gcctcgaccc ggcggcgctg gacgatctgc gcctcgaccc
     6961 ggacgacctg gcggatatgc ccggccgcct ccgccctcgg cactccgccg tagagcgcga
     7021 ggccgggccg ggtgaggtcg aaggcatagg cgggaccgag gcagatgccg gcgctgttgg
     7081 cgaggctccg gcgcttcgcc ggcacgcacg cggcgatctc ggcgaaagcg gcgcgctggc
     7141 gttcgttcat cgccacatcc tcgtcggcgc aggcgaggtg gctcatcagc gtatcgatct
     7201 cgaggccccc cagaagcccc gctgccacct cggccgggct gagaccgagc cggttgatgc
     7261 cggtgtcgat catgacgtcg cagggccgtc ccggcgccgc ctgcttccag cgctggacct
     7321 ggagcgccgt gttcagaacc ggccgggcgg gcgagccagc ggcgtgcgcc agatcctcct
     7381 cggcgaggcc gtggaggacg gagaccgagg cggcgccgag ccaggggagc agcgcctccg
     7441 cctcgggcca ggtggcgacg aagaaatcgc ggcagccttc gttggcgagc cgcctcgcca
     7501 cgtcgcgggc gccgaggccg tagccgtccg ccttcaccgc cgccccgcac gcggcgccgg
     7561 ccccctgccc cgccagccag cgccaattgg cgacgagggc gtcgccgtcc agcagaaggc
     7621 gcagcgggga atggggctgg ggcacgaggg cgcggatagc gggtgccgga cttcggggaa
     7681 accgtcattc cctcgaaagc ggagaagggc gttcgatcaa cccgccaaac gccttgcggc
     7741 aaggcctatt ccagctcctt gcccgccgtc tccggcagcc agaggaacgt gacgacgagg
     7801 gccatggcca ccaccccgac cgtgtaccac aggccggcga acggatcgcc ggtcctggcg
     7861 acgatatact ggctgatcaa gggcaggaat ccgccgaaat agccggtgcc gatatgatag
     7921 gggatcgaca tcgacgtgta gcggatccgc gccgggaaaa gctcgacgag aagcgcggcg
     7981 accggcccat aggtcattcc cgagcaaagg ccgatcagca cgatggcgag cacgacctgc
     8041 agcgcctggc cgaatgtcgg ggcgctcttc tcgagcttgt agccggcgat cttgaggccg
     8101 aaatcgagcg acgccggatc ggaggcgtcg accgcgacgc cgccgatcgt cacctgcggc
     8161 ggcgcgccgg cgggagcgtc ggccgtggaa taggcgatgc ccttcttgga aagcgcgtcg
     8221 agcagcttgc cgcagtcggt cggctgtacc ttggcgaacg gagcgtagct gcagtcgctt
     8281 ccggtcacga ccaccgggtt cttgcgcatc gcggcggaaa gctccggatt ggccgccgag
     8341 gccatccagt ggaacagggg gaagatcagg acgacggtga gcgcatagcc gatgacgatc
     8401 ggccccttgc gtccgacccg gtccgaaagc cagccgaaca ggatgaacca gatcatggag
     8461 atgacggccg cggccccgat catggcgcgg gcggcggtgt cctcgatcct cagcgaattc
     8521 tggagaaagt acatcgcctg gaactgggcg gtgtaccaga tgacggtcag gccggcggca
     8581 atcccgaaca gggcgacgag gacgagcttg atgttcgacc aggtcgcgaa ggtctccgcg
     8641 agggggttct tggccacctc cccggcttcc ttcatggcct ggaagacggg gctctccttg
     8701 agcttcagac gcacccagag cgagacggcg agaagcacga tggagaagag gaacgggatg
     8761 cgccagcccc acgcctccca ctgcgccttg tcgacgaaca gctcggatgc gaggacgacg
     8821 gcgacggaga ggatgaagcc gccgatcacc ccggcctgga tgaagctggt gtagaagccg
     8881 cgcctgttcc ggggcgcatg ctcggcgacg tagatcgcgg ccccgccata ttcgccgccc
     8941 agcgccagcc cctgcagcac cctcaggaag acgagcagag cgggggccgc gactccgatg
     9001 gccgcatagc cgggcagcag gccgaccgcg gcggtggcga tgcccatcag ggtgatggtg
     9061 acgaggaagg tgtacttgcg cccgagcttg tcgcccagat agccgaacag caccgcgccg
     9121 atcggccgca ctccgaagcc gactccgaag ctggcgagcg cgagcaggaa ctgggcggtc
     9181 tcgctggagg cggggaagaa ttgcttgccg atgaccgcgg cgagcgtccc gtagatgaag
     9241 aaatcatacc attcgaagac cgtgccgagc gtcgaggcga agatgaccag ccggtcccgc
     9301 ttcgcgtcga tcacgtagtc gccggatacc gccgtggcca tcaacgtccc ctgccctttg
     9361 cgcctgtgta agcgggccgg catggcaccg gcaagcgccc ctgaattcct ccccaggatt
     9421 tccgggggag ggggaccgcg cgcagcgcgg tggacgggtt cattcggatc tcggcaaggc
     9481 ccctccacca ggcttcgaat ggtccccctc cctgcaaaat gcaggggagg aacaagacgc
     9541 gtcagaccgc cagatgatcg agcaccgcgg gcagcagcac ctccgcggcc gcctcggcgg
     9601 cgcccggcag ggtgccgaac tcgccgtcta tggccagccg ggcgaagccg tggacggtcg
     9661 accagcaggc gatggagggg gccgtcccgg gcgcgcccgg ccttggcgcc ggcccctcgc
     9721 cgccgcgcac cgcatcgtcg aggagccgga aggcccgcgc ggcggcggcg gcatagtccg
     9781 gatcgtcgcg cttgagcaga gccgcccgga acatgaggtc gaagcgggct cggttcgcga
     9841 gggcgaagcg gacataggcg ctgccctgag cgcggatccg cgccgcgcgg ccgtccgccg
     9901 ccagcgccgc ctccagggcg tcgccgaggt cgcggaaggc gccggccgca accgcggtca
     9961 gcaggcccga ggcatcgccg aaatggtggg ccggagcggc cggggagacg cccgctcggc
    10021 gcgccgcctc gcgcaggctg aagccctggg tcccgcgctc ggccagcacc gcctcggcgg
    10081 ccgcgatcag cgccggccga agtgcgccgt gatgatagcc gcgaccggac ttgacactgt
    10141 tcagatcatc cgccataccg atctgtacaa tgtaaagatt ggagtggcaa cgtgctggac
    10201 ccgaacatcg ccttcggctt cgcgaacaat ttcgggctgg tcgcctggct cgccttgctc
    10261 gcctccctgt tcgtgccgcg cctgaggtcc tgggtgtggc cggcgacgca gttcgtcatc
    10321 ccggccgtct gggccgccgc ctacgtgctg ctcctcgtcc agggcatgcc ggagaccgac
    10381 gggagcttca gctcgatcga gggcgtgcgg agcctgttcg gcagcgactc cgctctgacc
    10441 gccggctggc tccactatct cgccttcgac ctgttcgtgg gcacctggat cgcccgcgat
    10501 tcgacggagc ggcggattca cggcctgctc gtcgtcccct gtctcgctct gaccctcgtc
    10561 ttcggcccat ccggcctcct cctctacctc ctcatgcgcc tcgccttcgg ccgccgcgac
    10621 ccgacggaga ccgcttcgtg accgcgctcg gccttcccct ccccctcacc cggctgccga
    10681 tcctgacgga actgctcgag cgccagcgcc tgctcgccct ctacggactg gcgaccctcc
    10741 tcctcgccct gccggtgctc gccctgcagg cgatcgaccc gcgcaccctt cacggcgtcg
    10801 gcgtctgggt gaagcccgcc aagttcctgg tctcggtcgg aatcttcgcg ctcaccgccg
    10861 cctggtattg gggctatgtc gcgcccgagc ggcgcgggag cggccccctg cgcctgaccg
    10921 cggccgtgct cgtcggctca gccagcttcg agcttctcta catcctcctc caggccgggc
    10981 gcgggctcga ctcgcacttc aacttcactt cgccgctcta cgtggcgatg tacggcctta
    11041 tggggctagc ggcggtgctg ctggtcggca ccgtccttcc cctcgcctgg cagatcgtcc
    11101 gccaccccgt gcccggcctg cgcggcgatt tcgtcgcggc gctggcgatc ggcctcgtcc
    11161 tcaccttcct gctcggcggc gccctcggcg gctatatgag cgcccaggcc ggccattcgg
    11221 tcggcgccca gggcggccac gtccccttct tcggctggaa ccggagcggc ggcgacctgc
    11281 gcgtcgccca cttcctcggc atccacgccg aacaggccct tcccctgttc gccgcttcga
    11341 tggccggccg gagcccgcgc atgcgctgga ccgggctggc gctcgccacg ctcgcctatg
    11401 tcgcgctcac cctcgccgtc ttcgcccagg ccgtggccgg aaagccgctg ctcgccctct
    11461 aggcctgttc ctcagcctgc gaggcccgat ctcgccgctt cccagttgcg cccgtcgaag
    11521 cttcgcaccg tcacctccag cgccgacacg tcgtcgaggg cgttgaggtt caggctccac
    11581 gcctcggggt gggagcgcgg ctggtagaag ctcttcacgc cgcagaccgc gcagaagaga
    11641 tgatccgccg cgccggtgcc gaagcgatag tgggtaagct tcgcctcgcc cgagacgagc
    11701 cggaagtccg aatgggggac gatgaggtgc ctgaagccgg tcatcgcgca gatcgagcaa
    11761 ttgcagtcca gcacctccac cgccgccggc acccgcgcct cgaagcggac cgctcggcaa
    11821 tggcaaccgc cggagatcgg gaccggattc acggcgagat gctcagcgcg acggcggctt
    11881 gcgcggaggg cggcctccgc ctttgcccgg agggcggccc ggcccggccc cgtcgcgctt
    11941 gggcggcggc cggcggaacg gccggtgctg ggaggccggc gggcctcccg ggcgcggcgg
    12001 gcgcggcgcg gcggcctcgc cgccggcgac cgcatagcct tccttctgga gtcgcgagaa
    12061 ggcggcgcga acgct
//
LOCUS       DASVQZ010000101         8386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_119394, whole genome shotgun sequence.
ACCESSION   DASVQZ010000101 DASVQZ010000000
VERSION     DASVQZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119394"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF547_02765"
     CDS             complement(<1..720)
                     /locus_tag="VF547_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261776.1"
                     /translation="MADADIVGNIAEPGGDAHPTPPRGRRRGSRDPKVFVLDRELSEV
                     HLLLDNVSANPATSLAERIAAADGLEDVLPDDWIEQVCRISWPPDGTEEDKAEDAALL
                     IKAKDRLNRLSYPASGSTIAFTLLVTQHDTQSEPWLRRRDGGEPAETRSPTRNSLAQT
                     AYPDLLPKAVRFRKAMRSISIGLVAALFVTCLLSWYVAYGNSALAELSAAESALETVD
                     KRVDDAEAAEDSQRQGPAAALT"
     gene            complement(939..1406)
                     /locus_tag="VF547_02770"
     CDS             complement(939..1406)
                     /locus_tag="VF547_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261777.1"
                     /translation="MADPDPFALPPRLAALIEDLPLDDLAFTPVPVRARRDGWSPECQ
                     RAFIIRLALCGSASTAARAVGKTRRSAYRLREHEGAESFALAWDKAARWGQNSRLDFA
                     LERAIAGEVRPYYYRGRKCGENIVYSEGLSVAVLKLMGRRFGDPEKKRFFGPR"
     gene            1543..2208
                     /locus_tag="VF547_02775"
     CDS             1543..2208
                     /locus_tag="VF547_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX8261778.1"
                     /translation="MRKPRRSRAPANQELLRSFSIDHAERVTGLSKLRLARWDRLGLF
                     SPEAVDPGDKGNPYARVYTFNDLVGLRTLALLVGKYRVPIREIRGAYKKLAKLVKSPW
                     SDTPLSVCKRQIVFDLHGQPRNVTDGQAVLKHIPLPEIADEVAKRTEALRKRRKEQIG
                     TTERRKFFVHNAIVLAGTRIPVRAIESFIGEGYSDKAIVEEYPSLTRADVAMVRRQMS
                     ARI"
     gene            complement(2668..3336)
                     /gene="purQ"
                     /locus_tag="VF547_02780"
     CDS             complement(2668..3336)
                     /gene="purQ"
                     /locus_tag="VF547_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX8261779.1"
                     /translation="MKSAVIVFPGSNCDRDLAVAIEAVTGSRPAMVWHRETELPAGLD
                     LVAVPGGFSYGDYLRSGAMAARSAIMRAVGEAAARGVPVLGVCNGFQILTEAGLLPGA
                     LMRNAGLHFVCRDVPLKVENSQTAFTSRYEAGEAITIPVAHHDGNYQADPETLDRLEG
                     EGRVAFRYADEVNGSARGIAGIVSEAGNVLGMMPHPERMVEPAHGGADGRRLFEGLLE
                     AVGA"
     gene            complement(3333..3584)
                     /gene="purS"
                     /locus_tag="VF547_02785"
     CDS             complement(3333..3584)
                     /gene="purS"
                     /locus_tag="VF547_02785"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX8261780.1"
                     /translation="MKTRVYVTLKNGVLDPQGKAIHHALEGLGFSGVRDVRAGKLIEL
                     DLDDGVGDSEVEAMCRKLLANTVIENFRIEREAPAERPA"
     gene            3683..3832
                     /locus_tag="VF547_02790"
     CDS             3683..3832
                     /locus_tag="VF547_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261781.1"
                     /translation="MVGLALMMFLALVAFGSQRLGWSDPDGQVQLGIFMSSLFGIICG
                     CKTRG"
     gene            3899..4234
                     /gene="erpA"
                     /locus_tag="VF547_02795"
     CDS             3899..4234
                     /gene="erpA"
                     /locus_tag="VF547_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX8261782.1"
                     /translation="MAEPSEIALTPAAAARVAAIAERQGKPAILRLSVEGGGCAGFSY
                     RFGLADEVESDDLVAGEGGVTLVVDPMTHDLVKGGAVDYVESLGGAAFQVRNPNAASG
                     CGCGSSFSV"
     gene            4344..5129
                     /gene="xth"
                     /locus_tag="VF547_02800"
     CDS             4344..5129
                     /gene="xth"
                     /locus_tag="VF547_02800"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8261783.1"
                     /translation="MKIVTYNLNGIRARLPRLVEYLEEEKPDALCLQEIKCADEAFPI
                     EDVRAAGYDGVWHGQKGFNGVAILTPAAAPAPALRRVGLPGDPDDTHSRYIEAEVGGV
                     TIGSIYLPNGNPVGTEKFAYKLAWMERLREHASALLAEERPVVLAGDWNVIPTDSPDD
                     VFSARAMRHDALMQPESRAAFRRILHAGWTDAIRARHPDGPVYTFWDYTAGCWQRDAG
                     FRIDHLLLSPHAADRLLDAGVDKSYRGREKASDHAPTWVRLEK"
     gene            5167..7002
                     /locus_tag="VF547_02805"
     CDS             5167..7002
                     /locus_tag="VF547_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8261784.1"
                     /translation="MQPLTRRHFLGSAGAVAALQLLPGCATRGASEAAPPVAAMLSAM
                     AEQLLAEYPENATALGLDKDARAGLKSRLTDRSLHGRARLAGAAADRLRRIGAVDRAG
                     LEPADRIHLGVVETAHRLAVEGFRFPYGDVVTLNQQWSYRNAPYVVAQNTGAFVEIPD
                     FLDSNHTVAAAADAEAYLARLEAYAEALDGETERLAHDRGLGVVAPDFLLDKTLKQMR
                     SARSQPVPEWGLVTSLERRTTSIPGDWRGRAEALARGKVAPAMDRQIAELARHRARAD
                     GRAGVWKLPQGEDYYAWALRAATTSSLTPDEVHRMGLEQLAELQGRMEPLLRAQGLTR
                     GTVGERMTALGNDPRHLFPNTDAGRQQILAFVNERVADIRNRLPRAFATLVPGRLIIK
                     RVPPEIEAGAPGGYAAAGTIDGSVPGNYYINLRDTSMWPRFSLATLTYHEGIPGHIWQ
                     GEYTYKLPLIRSLLAFNAYSEGWALYAEQLADELGAYEGDPLGELGYLQSLSFRACRL
                     VVDSGLHAQRWTRAKAIQWFAETNGSTVEEVQGEVDRYCAWPGQACGYKVGHSEIVRL
                     REKAKAALGDRFDLRRFDDALVLGGNVPLTLLEGVVDRHVAAARS"
     gene            7160..8026
                     /locus_tag="VF547_02810"
     CDS             7160..8026
                     /locus_tag="VF547_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261785.1"
                     /translation="MTAIAALGWAALAPAQDAVPREVDLEIIAGEDWAATGIEVRPGD
                     RISIEAGEAPSPHGPKSPDGEPDPVAENVLEPRLPYAALIGRIGEGESFFVGARYKGR
                     MSAAGPLRLRWNLIRRPIARFAEVFPTKVRHRPGRKVDKDRTDSADKKGGDKAPVDKA
                     PVDEARVDEAPVEPVVEAPPPVAATPPATAKPVPARAVQPRPAAASSRWPWWLALGVA
                     GAGAAGLALQRTGRARRVRRTRALLGLTPSLDLADGRCRGDDLALEGPSASLRARLEP
                     GIAHMEEEGGHG"
     gene            8019..>8386
                     /locus_tag="VF547_02815"
     CDS             8019..>8386
                     /locus_tag="VF547_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261786.1"
                     /translation="MVEGSGITVADLLPDRFDGLEEQVKARLADDGDTGAGKLAWDLI
                     GAEIEGALRSVLDCDLIEALAGAWKSARALQDYADPAKHPPGEASVVHLGERKVEREL
                     HPVVGVTIGARECMELRFTLA"
BASE COUNT         1339 a         2849 c         2898 g         1300 t
ORIGIN      
        1 cgtcaaagcc gccgcaggcc cctgacgctg gctgtcctcg gccgcttcgg cgtcgtcgac
       61 ccgcttgtcg accgtctcga gcgcgctctc ggcggcgctc agctcggcga gggcggaatt
      121 accgtaggcg acgtaccagg agagcaggca ggtgacgaac agggcggcga ccaggccgat
      181 gctgatcgag cgcatcgcct tgcggaagcg gaccgccttg ggaagaagat ccggataggc
      241 ggtctgggcg agcgagttgc gggtcggcga ccgcgtttcg gcgggctcgc cgccgtcccg
      301 ccggcgaagc cagggctcgc tctgggtgtc gtgctgggtg acgagcaagg tgaaggcgat
      361 ggtcgatccg ctcgccggat aggacagccg gttgagccgg tccttggcct tgatcagcag
      421 ggccgcgtcc tccgccttgt cctcctcggt tccgtcgggg ggccagctga tccggcagac
      481 ctgctcgatc cagtcgtcgg gcaggacgtc ctccagtccg tcggcggcgg cgatgcgctc
      541 ggccaggctg gtggccggat tggccgagac gttgtcgagc agcaggtgga cctcgctcag
      601 ctcgcggtcg aggacgaaga ccttggggtc gcggctgccc cgccggcggc cgcgaggcgg
      661 agtcggatgg gcgtcgccgc ccggttcggc aatattaccg acaatgtccg catcggccat
      721 gtgcctaccc cctcaacggc cgcgaagagg gcgcatagtc gtcgcgccat tcgcgtgccc
      781 agcgcggtag aatgacatcg tgcgacaatt gctgcaaccc caggtcttta cttgccccgt
      841 tcaggcggcg gcgcccggcc cggacgcggg acttcgtgct gaaagccgcg cccgaggacg
      901 cggtcgcaaa aggagtctcg aaaagacaca aaaacccctc atctcggccc gaaaaatcgt
      961 ttcttttcag ggtcgccgaa gcgccgcccc atgagcttga gaacggcgac gctgagcccc
     1021 tcgctgtaga cgatgttttc gccgcacttg cgcccgcgat aataataagg ccgcacctcg
     1081 ccggcgatgg cgcgctccag cgcgaaatcg agacggctgt tctgccccca tcgagcggcc
     1141 ttgtcccagg cgagggcgaa gctctccgcc ccctcatgct cgcgcaggcg atatgcgctc
     1201 cgccgggtct tgcccacagc ccgcgcggcg gtgctcgccg aaccgcagag cgcaaggcga
     1261 ataatgaagg cacgctggca ttccggcgac cacccgtccc gccgtgcgcg caccgggacg
     1321 ggcgtgaagg cgagatcgtc gagcggaagg tcctcgatga gcgcggcgag gcgagggggc
     1381 agcgcgaagg ggtccggatc ggccatcaag cgaggctagc attcactaat gttcttgtaa
     1441 agttcttttt tagttctaag aggtggcatt gtcgcccact ggcgccgccc atgcgatttt
     1501 agccctccac atgggagcgg cggtagtata ggaattcagg ttgtgagaaa gcccagacgg
     1561 tctagagccc ctgcaaatca ggaacttcta cgctctttca gtatcgatca tgcagagcgc
     1621 gtaaccggcc tatctaagct tcgacttgcc cgttgggata ggcttggcct gttttccccg
     1681 gaggcagtgg atccaggtga caagggtaac ccctatgctc gggtatacac tttcaacgac
     1741 ctggttggac tgcgcacgct ggctcttttg gtagggaaat accgcgtacc gataagggaa
     1801 attcgcggtg cgtataagaa gctcgccaag cttgtcaaaa gcccttggtc ggacaccccc
     1861 ctctctgttt gcaagcgcca aatcgttttc gacttacacg gtcaaccgcg aaatgtgacc
     1921 gatggtcagg cggttctaaa acatatcccg cttccggaaa tcgcagacga agtggcaaag
     1981 cggacagagg cgctacggaa acgtcgcaag gagcagatcg gaacgacgga gcggcgcaag
     2041 tttttcgttc ataatgcgat cgtgctggcg ggaacgcgca tcccagttag agcaatcgag
     2101 agcttcatcg gagagggcta cagcgacaag gcaatcgttg aagaataccc ttcgctgaca
     2161 cgcgccgatg tggcgatggt gcgccgacag atgagtgctc gcatctgacc ggcggccgaa
     2221 agctgcgatt ttttgctgat cattgtgtgg cggaatctgt ctgtcgtttc ctggaggaac
     2281 gagggcacga ggttgtcagg ctacgttcga tactgccaac cgatacgcct gaccccattg
     2341 tagcgaagac ggcccaggac ctggaggcca tcctgataag cgacgatcag gacttcaacg
     2401 tcatcgccgg tcgtaggccc acggcgaggc agcgccgctt ccagcggtta agccgagtta
     2461 atatgcggtg tccgcatgct aaagcggtcc aacggctcgc cgccgctatc gacctgattg
     2521 agttcgaata cgaagccgct caatcgcggc cagacagacg aatgatcatc gatattcaac
     2581 cgaccttaat ccgcaccctt cgctaagccg aagccggcgg ttcgggctcg ttcgctgcga
     2641 ttagatcagt gataaatgac gatcgatcta ggccccgacc gcctcgagca ggccctcgaa
     2701 caggcgccgc ccgtcggctc cgccgtgggc cggctccacc atccgctcgg ggtgcggcat
     2761 cattccgagg acgttgccgg cctcgctgac gattccggcg atgccgcggg cggaaccgtt
     2821 gacctcgtcg gcgtagcgga aggcgacccg gccctcgcct tcgaggcggt cgagcgtctc
     2881 ggggtcggcc tggtaattgc cgtcgtggtg ggcgaccggg atggtgatcg cctcgccggc
     2941 ctcgtagcgg ctggtgaagg cggtctggct gttctcgacc ttgagcggga cgtcgcggca
     3001 gacgaagtga aggccggcgt tgcgcatcag ggcgcctgga agcagccccg cctcggtgag
     3061 gatctggaag ccgttgcaca caccgagcac cggtacgccg cgtgccgcag cctcgcccac
     3121 ggcgcgcatt atggcagagc gggcggccat tgcccccgat cgcagatagt cgccgtagga
     3181 gaagccgccg gggacggcga cgaggtcgag gccggcgggc agctccgtct cgcggtgcca
     3241 gaccatcgcc gggcggctgc cggtcaccgc ctcgatcgcc acggccaggt cgcgatcgca
     3301 attcgagccg gggaagacga tgacggcgct tttcacgccg ggcgctccgc cggcgcctcg
     3361 cgctcgatcc ggaaattctc gatgacggtg ttggcgagca gcttgcggca catcgcctcg
     3421 acctcggaat cgccgacgcc gtcgtccagg tcgagctcga tcagcttgcc ggccctcacg
     3481 tcccggacgc cgctgaagcc gagcccctcg agcgcgtggt ggatggcctt gccctgcggg
     3541 tcgagcacgc cgttcttcaa ggtcacgtag acgcgggtct tcatcgccgc cctatgccgc
     3601 caaagcggcc gcgggcacaa gcgttcaccc cgccgtttgc ggccttttgc gatcgtcgag
     3661 gcgcaagaag cgggaaagcg ccatggtcgg cctcgcattg atgatgttcc tggccctggt
     3721 cgccttcggg tcgcagcggc tcgggtggtc cgacccggac ggccaggtcc agctcggcat
     3781 cttcatgtcg tccctgttcg gcatcatctg cggctgcaag acccgcggct agccgagggc
     3841 gtccttcccc gaatagcgct cgcttgtggg ggaggcgacg gtcccctatc ttgaggccat
     3901 ggccgagcct tccgaaatcg ccctcacgcc cgccgccgcg gcccgggtcg ccgcgatcgc
     3961 cgagcggcag ggcaagccgg ccatcctgcg cctcagcgtc gagggcggcg gctgcgccgg
     4021 cttcagctac cgcttcggac tcgccgacga ggtcgagagc gacgacctcg tcgcgggcga
     4081 gggcggagtc accctggtcg tcgacccgat gacccatgat ctggtcaagg gcggagcggt
     4141 cgattacgtc gagagcctcg gcggcgccgc cttccaggtg cgcaacccga acgcggcatc
     4201 cggctgcggc tgcggatcca gcttcagcgt ctagcgtcgt cccagcgaag gcgggacccc
     4261 agcgagaacg ggcgcggcgc ttttccgccg cgggatgcca gccttcgctg gcatgacggg
     4321 aagggcgccg ctatccggct tccatgaaga tcgtcaccta caatctgaac ggcatccgag
     4381 cgcggctgcc gcggctggtc gagtatctcg aggaggagaa gcccgacgcg ctctgcctcc
     4441 aggagatcaa gtgcgccgac gaggcctttc cgatcgagga cgtccgggcg gccggctacg
     4501 acggcgtgtg gcacgggcag aaggggttca acggagtcgc catcctcacc cccgcggccg
     4561 cgccggcgcc ggcgctgcgc cgcgtcgggc ttccgggcga cccggacgac acccacagcc
     4621 gctacatcga ggccgaggtc ggcggagtga cgatcggctc gatctacctg cccaacggca
     4681 atccggtcgg gaccgagaag ttcgcctaca agctcgcctg gatggagcgg ctccgcgagc
     4741 atgcttcggc gctgctggcc gaggagaggc cggtcgtgct ggccggcgac tggaacgtca
     4801 tccccaccga cagccccgac gacgtcttct cggcgcgggc gatgcggcac gacgcgctga
     4861 tgcagccgga gagccgggcc gccttccgcc ggatcctcca cgccggctgg accgacgcca
     4921 ttcgggcgcg ccatccggac gggcccgtct acaccttctg ggactatacc gccggctgct
     4981 ggcagcgcga cgccggcttc cgaatcgacc acctcctgct ctcgccgcac gcggccgacc
     5041 ggctgctcga tgccggagtc gacaagagct atcgcggccg cgagaaggcc tcggaccacg
     5101 ccccgacctg ggtgaggctg gaaaagtgac cgtcaccagt gacggtcagc aatcggaagg
     5161 atcgcgatgc agcctttgac ccgccgccat ttcctcggct cggccggagc cgtcgccgcg
     5221 ctgcagctgc ttcccggctg cgcgacccgg ggcgcttcgg aagcggcgcc gccggtcgcg
     5281 gcgatgctga gcgccatggc cgagcagctg ctcgccgaat atcccgagaa cgccaccgcg
     5341 ctcggcctcg acaaggacgc ccgagcgggc ctcaagtcga ggctcacgga ccgctcgctg
     5401 catggccggg cgcggctggc gggcgcggcc gcggaccggc tgaggcggat cggcgcggtg
     5461 gaccgagccg ggctcgagcc cgccgaccgg atccatctcg gagtcgtcga gacggcgcac
     5521 cgcctcgccg tggagggctt ccgcttcccc tatggcgacg tcgtcaccct caaccagcaa
     5581 tggtcctacc gcaatgcgcc ctatgtcgtg gcccagaaca ccggcgcctt cgtcgagatt
     5641 ccggacttcc tcgacagcaa tcacacggtc gcggcggcgg ccgacgccga ggcctatctg
     5701 gcgcggctcg aggcctatgc cgaggcgctc gacggcgaga ccgagcggct cgcccacgac
     5761 cgcggcctcg gcgtcgtcgc gcccgatttc ctgctcgaca agacgctcaa gcagatgcgg
     5821 tccgcccgct cgcagccggt gccggaatgg ggcctggtca cctcgctcga acgccgaacg
     5881 acgtcgatcc cgggggactg gcgggggcgg gccgaggccc tggcccgcgg caaggtcgct
     5941 ccggcgatgg accggcagat cgccgagctc gcccgccacc gcgcccgcgc cgacggccgc
     6001 gccggagtct ggaagcttcc gcaaggcgag gattattacg cctgggcgct tcgggcggcg
     6061 acgacgagca gcctgacgcc cgatgaggtc caccggatgg gcctcgagca gctcgccgag
     6121 ctgcagggcc ggatggagcc gctgcttcgc gcccagggcc tgacgcgggg cacggtcggc
     6181 gagcggatga cggcgctcgg caacgatccc aggcacctct tcccgaacac cgacgccggc
     6241 cgccagcaga tcctcgcctt cgtcaacgag cgggtggccg acatccgcaa ccgcctgccg
     6301 cgcgccttcg cgaccctcgt tccggggcgt ctgatcatca aaagggtgcc gccagagatc
     6361 gaggccggcg ctccgggggg ctatgcggcg gccggcacga tcgacggctc ggtgccgggc
     6421 aattactaca tcaaccttcg cgacacttcg atgtggccgc gcttcagcct cgccacgctg
     6481 acctatcacg agggcattcc gggccatatc tggcagggcg aatataccta caagctcccg
     6541 ctgatccgat cgctgctcgc cttcaacgcc tattcggagg gctgggcgct ctacgccgag
     6601 cagctcgccg acgagctcgg agcctatgag ggcgatccgc tcggcgagct cggctatctc
     6661 cagtcgctct ccttccgcgc ctgccggctg gtcgtcgact ccggcctcca cgcccagcgc
     6721 tggacccggg cgaaggcgat ccagtggttc gccgagacca acggctcgac cgtggaggag
     6781 gtgcagggcg aggtcgaccg ctattgcgcc tggcccggcc aggcctgcgg ctacaaggtc
     6841 gggcacagcg agatcgtccg gctgcgggag aaggcgaagg cggcgctggg cgaccgcttc
     6901 gacctgcgca ggttcgacga cgctctggtg ctcggcggca acgtcccgct gaccctgctc
     6961 gagggcgtgg tcgaccgcca tgtcgcggcc gccaggtcct agcgccgaaa cggaccagaa
     7021 ggcggccgcg cgcggattga ctccgccggt tcccgggcgg actctcctcg gcgcggcgag
     7081 ggggccgcgc ggcatggcga tcccggaacc gctggcacgg gtctggcgtg ggaggcgagg
     7141 acggagcctg gtcctggcat tgacggcgat cgcggcgctc ggctgggcgg ccctcgcccc
     7201 cgcccaggat gccgtcccgc gcgaggtcga cctcgagatc atcgccggcg aggattgggc
     7261 ggcgaccggg atcgaggtcc ggcccggcga caggatctcg atcgaggcgg gcgaggcgcc
     7321 gtcccctcat ggccccaagt ctcccgacgg ggagcccgat ccggtggccg aaaacgtcct
     7381 cgagccgcga ttgccttacg ccgccctgat cgggcggatc ggcgagggcg agagtttctt
     7441 cgtcggggcc cgctacaaag gcaggatgag cgcggccgga ccgctccggc tccgctggaa
     7501 cctgattcgc cggcccattg cccgcttcgc cgaggtgttc ccgacaaagg tccgccacag
     7561 gccgggccgg aaagtcgaca aggacaggac ggactcggcc gacaagaagg gcggggacaa
     7621 ggcgccggtc gacaaggcgc cggtcgacga ggcgcgggtc gacgaggcgc cggtcgagcc
     7681 ggtcgtcgag gcgccgccac ctgtcgccgc gacgccgccc gccaccgcga agcccgtccc
     7741 ggctcgggct gtccagcccc gccccgccgc ggcgtcgagc cgctggccct ggtggctcgc
     7801 tttgggcgtg gccggggccg gcgccgccgg actggcgctc cagcggacgg ggcgcgcccg
     7861 gagggtccgg cggacccgcg cgctgctcgg cctgacgcct tcgctcgacc tcgcggacgg
     7921 ccgctgccgc ggcgacgacc tcgcgctcga aggaccgtcg gcctcgcttc gggctcggct
     7981 cgagccgggc atcgctcaca tggaggagga aggcgggcat ggttgagggg tcggggatca
     8041 ccgtcgcgga cctcctgccc gaccggttcg acgggctcga ggagcaggtc aaggcgcggc
     8101 tcgcggacga cggcgacacc ggcgccggca agctcgcctg ggacctgatc ggcgccgaga
     8161 tcgagggcgc gctgcgctcg gtgctcgact gcgacctgat cgaagcgctg gccggggcct
     8221 ggaagtcggc ccgggccctc caggactatg ccgatccggc caagcatccg ccgggcgagg
     8281 cctcggtggt ccatctcggc gagcgcaagg tggagcgcga gcttcatccc gtcgtgggcg
     8341 tgacgatcgg cgcccgtgaa tgcatggagc ttcgcttcac gctcgc
//
LOCUS       DASVQZ010000102         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_694482, whole genome shotgun sequence.
ACCESSION   DASVQZ010000102 DASVQZ010000000
VERSION     DASVQZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694482"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF547_02820"
     CDS             <1..181
                     /locus_tag="VF547_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261787.1"
                     /translation="ILFGAPAIAAALHGLAGGGAPAAAVAEPAWAPPPLPVPPPPPPQ
                     APNPDPYAGASVPAR"
     gene            263..400
                     /locus_tag="VF547_02825"
     CDS             263..400
                     /locus_tag="VF547_02825"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX8261788.1"
                     /translation="MAAQGELEKEVVTHKKGYEGFVFLMKWGAIICMVIALIVILLIS
                     E"
     gene            408..1535
                     /locus_tag="VF547_02830"
     CDS             408..1535
                     /locus_tag="VF547_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX8261789.1"
                     /translation="MKLAVLKETAPAERRVAATAETVRKFAALGAEVAVEAGAGLAAS
                     VADADYRAAGATPGPRDAVLAGADAVLAVQAPDAAALGGAKPGALAVALFDPFRSRER
                     VEGYAAAGLEALAMELMPRITRAQSMDVLSSQANLAGYKAVLDAAAEYGRAFPMMMTA
                     AGTIPAARVFVMGVGVAGLQAIATARRLGAVVSATDVRAATREQIESLGAKAVFVETV
                     AGIEGEGSGGYATEMSDEYKAAQAELVSGHIAKQDIVVTTALIPGRPAPRLISDAQLA
                     SMRPGSVVVDLAVEQGGNVEGSSPGEVAERHGVRIVGHRNVPSRLAADASALYARNLY
                     NFLSAFWDKEAGRPVLPDEDEVTQGVRLTKDGQVVCARLLG"
BASE COUNT          209 a          589 c          613 g          209 t
ORIGIN      
        1 catcctgttc ggagcgccgg cgatcgccgc cgccctccac ggcctcgccg gcggcggggc
       61 gccggcggcg gccgtggccg agccggcctg ggcgccgccg ccgcttcccg tgccgccgcc
      121 gccgccgccg caggcgccca acccggatcc ctatgcgggc gcgtcggtgc cggcgcgctg
      181 agcccggggc cgcgcgttgc ggccttccca cgccgccgcc gcgttgataa gaggtcgccg
      241 cacccaaggc acgaggacgg acatggcggc gcaaggcgag ctcgagaagg aagtcgtcac
      301 ccacaagaag ggctatgagg gcttcgtctt cctgatgaag tggggcgcca tcatctgcat
      361 ggtcatcgcc ttgatcgtca tcctgctgat cagcgaataa gccggcggtg aagcttgcgg
      421 tcctgaagga gacggcgccg gccgagcgca gggtcgcggc gacggccgag accgtgagga
      481 agttcgccgc gctcggagcc gaggtcgcgg tcgaggccgg ggccgggctc gccgcctcgg
      541 tcgccgacgc cgactaccgg gccgccgggg cgactcccgg cccgcgcgac gcggtcctcg
      601 ccggcgccga cgccgtcctc gccgtccagg cccccgacgc ggccgcgctc ggcggggcga
      661 agccgggcgc gctcgcggtc gccctgttcg atccgttccg gagccgcgag cgggtcgagg
      721 gctatgccgc ggccggcctc gaggcgctgg cgatggagct gatgccgcgc atcacccgcg
      781 cccagtcgat ggacgtgctc tcgagccagg ccaatctcgc cggctacaag gcggtgctcg
      841 acgcggccgc cgaatatggt cgggccttcc cgatgatgat gaccgcggcg gggacgatcc
      901 cggcggcgcg ggtcttcgtc atgggcgtcg gggtggccgg gctccaggcg atcgccaccg
      961 cccgccggct gggcgccgtc gtctcggcga ccgacgtccg ggccgccacc cgcgagcaga
     1021 tcgagagcct cggcgccaag gccgtgttcg tcgagacggt ggcggggatc gagggcgagg
     1081 gctccggcgg ctacgcgacc gagatgtcgg acgagtacaa ggcggcgcag gcggagctgg
     1141 tctcgggcca catcgccaag caggacatcg tcgtcaccac cgccctcatc ccgggccggc
     1201 cggcgccgcg gctgatctcc gacgcccagc tggcctcgat gcggccgggc agcgtcgtcg
     1261 tcgacctcgc ggtcgagcag ggcggcaatg tcgaaggctc ctcgccgggc gaggtggcgg
     1321 agcggcatgg ggtgcggatc gtcggccacc gcaacgtccc ctcgcgcctc gccgccgacg
     1381 cctcggccct ctacgcccgc aacctctaca atttcctctc cgccttctgg gacaaggagg
     1441 ccggccggcc ggtgctcccc gacgaggacg aggtgaccca gggcgtgcgg ctgaccaagg
     1501 acgggcaggt cgtctgcgcg aggctgctgg gatgatcgcc gccattcctc cccatgcccc
     1561 gcatggggag ggggaccatg cgcagcatgg tggaggggca tcgacggtgc ggatggcccg
//
LOCUS       DASVQZ010000103         5249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_216704, whole genome shotgun sequence.
ACCESSION   DASVQZ010000103 DASVQZ010000000
VERSION     DASVQZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216704"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VF547_02835"
     CDS             complement(<1..412)
                     /locus_tag="VF547_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase"
                     /protein_id="HEX8261790.1"
                     /translation="MKIGKIETFFVPPRWLFVRVESDEGAVGWGDASLEGWAEAVDGA
                     FAALRDRFLGHDPFRIEDVFQIGYRGGFYRGGPVLMSALAGLEQALWDLKGRAFGLPA
                     WEMMGGRVRDRVRAYAWIGGDRPHEIGEAAAARRA"
     gene            complement(542..1774)
                     /locus_tag="VF547_02840"
     CDS             complement(542..1774)
                     /locus_tag="VF547_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX8261791.1"
                     /translation="MEVCGHYRHWRTDFDCCDELGIHFLRYGPPLYRTLLGPNTYDWE
                     FADLTFAELKRRNLTPIVDLCHFGVPDWIGNFQNPDFPQLFATYAGAFADRFPWVQLY
                     TPVNEMFICAIFSAKYGWWNEQAKDDRSFVTALKHIVKANVLAMIEILKRRPDAIFIQ
                     SESSEYFHADSPSAIHKAELMNSRRFLSLDLNYGRRVDSEMYEYLMDNGMSREEYHFF
                     LNHRLKQHCILGNDYYMTNEHRVYADGHTESSGEVFGYSEITRQYYNRYRLPVMHTET
                     NLREGPKGDEAVQWLWKEWANVLRIRNVGIPTVGFTWYSLTDQVDWDTALRQQNGNVN
                     PLGLYDLDRNIRNVGRAYKKLISDWRDVLPASSLCLSVPIVAPAHTDRPFAREQKAEA
                     AKHLKGFVTESANPEAGE"
     gene            2048..2470
                     /locus_tag="VF547_02845"
     CDS             2048..2470
                     /locus_tag="VF547_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8261792.1"
                     /translation="MRLSRAQMDAKMDAHFAFEAQDDVEGVLATLTDDAEHDIVGWPG
                     GPTRGREGPRGFYETLFGDLADGRVETLRRYYGDDFMVDESLWRGRAPGRPFGIEGRG
                     RPLEFRLLHVVEFDEAGDMKRENVWLDLAAIQRQLQDV"
     gene            2470..3078
                     /locus_tag="VF547_02850"
     CDS             2470..3078
                     /locus_tag="VF547_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8261793.1"
                     /translation="MAQTGSGGRPNQRRRTRKDLLEAAARLMRRGKSPTLDEIAEEAL
                     VSRATAYRYFPGVEALLLEASLDVAFPDAAALFAEGPLDVAARVEAAEAAVSEMVREN
                     EPALRTMLANALQARPKAEPDDLPARQNRRSQLIDAALAPGAFAPAARDRLAKALAFF
                     IGTEAMLVGKGVLQLDEAEAAAVKRWAMRSLVTAAARADSDT"
     gene            3142..3264
                     /locus_tag="VF547_02855"
     CDS             3142..3264
                     /locus_tag="VF547_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261794.1"
                     /translation="MASAAPSLKGEWTYRSFANEPKPVDGNAQAALGLIFGEGS"
     gene            3327..3590
                     /locus_tag="VF547_02860"
     CDS             3327..3590
                     /locus_tag="VF547_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261795.1"
                     /translation="MDLIGSFRPAGAGAPAVIAARGTGRKGSGIEDFVYDYIFYPAPA
                     WPAGIGQRQALVGTVLRAADHGGAKKGAVVSAIAVRRGSKPAG"
     gene            3677..4564
                     /locus_tag="VF547_02865"
     CDS             3677..4564
                     /locus_tag="VF547_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX8261796.1"
                     /translation="MRWDEGFIAVDWGTTNRRAWRLGPGGAVAAEMEDDQGILAVPKG
                     GFHSAVGAVRARLGDLPMLMAGMIGSNRGWIEADYVPCPAGLPDLAARLRWIDGERAA
                     IVPGVSFDGDGRADVMRGEEVQLLGAYAEGWIDGDASLCHPGTHNKWVRLADGRIAAF
                     RTVMTGEMFNLLREHSILADLLAAPAEPGAAFDAGVRHGLDRDDLIAELFSVRARVLL
                     GKAPREEAAAYVSGLLIGADLKVGLRLVGDGEIVAMGRPELTRLFAAALAVAGRPARA
                     VDGEAAFLAGVRHLAELVQ"
     gene            4561..5196
                     /locus_tag="VF547_02870"
     CDS             4561..5196
                     /locus_tag="VF547_02870"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX8261797.1"
                     /translation="MTPDEELGRRLSECPLVAIIRGVTPGEAEAIGAAIVEAGIGIVE
                     VPLNSPDPLASIERLARSLGDSALIGAGTVLDPFDVGRVRDAGGRIIVSPNTFPPVIE
                     ASAAAGLVSLPGYFTPSEAFAALRAGATALKLFPAEGASPAVVRAQRAVLPKGVPLLV
                     VGGIAPDNMGPWLEAGASGFGLGSGLYKPGQSAEETAAKARAYVEAVQRSA"
BASE COUNT          807 a         1726 c         1872 g          844 t
ORIGIN      
        1 gggcccggcg ggccgcggcg gcctcgccga tctcgtgcgg gcggtcgccg ccgatccagg
       61 cataggcccg cacccggtcg cggacccggc cgcccatcat ctcccaggcg ggaaggccga
      121 acgcccgccc cttgaggtcc cacaaggcct gctcgagacc cgccagcgcc gacatgagga
      181 cggggccgcc gcggtagaag ccgccgcggt agccgatctg gaagacgtcc tcgatccgga
      241 acgggtcgtg gccgaggaag cggtcgcgaa gggccgcgaa ggcgccgtcc accgcttccg
      301 cccacccttc caggctcgca tcgccccagc cgaccgcgcc ctcgtccgat tcgacgcgga
      361 cgaacagcca gcgtggcggg acgaagaagg tttcgatctt gccgatcttc atgctttacc
      421 tcgggtcctt cgatatcggc cttcgcctcg ctcagtccct acgcaggacg agcggtggag
      481 ttacgccaat ccgctcatcc tgagtagccg ctgagcgaag tcgaagcggc gtatcgaagg
      541 atcattcccc cgcctccgga ttggcggact cggtcacgaa gcccttgaga tgcttggcgg
      601 cctccgcctt ctgctcgcgg gcgaacgggc ggtcggtatg ggccggcgcg acgatcggca
      661 ccgacaggca caggctcgag gcgggcagga cgtcgcgcca gtccgagatc agcttcttgt
      721 aggcgcggcc gacgttgcgg atgttgcgat cgagatcgta gaggccgagc ggattgacgt
      781 ttccgttctg ctggcggagc gccgtgtccc agtcgacctg gtcggtcagc gaataccagg
      841 tgaagccgac ggtcgggatg ccgacgttgc ggatccggag cacgttcgcc cattccttcc
      901 agagccactg aaccgcctcg tcgcccttgg gcccttcgcg aagattggtt tcggtgtgca
      961 tcaccggcag ccggtagcgg ttgtaatatt ggcgcgtgat ctcgctatag ccgaatacct
     1021 cgcccgagct ctcggtatgc ccgtcggcat acacccgatg ctcgttcgtc atgtaataat
     1081 cgttgccgag gatgcagtgc tgcttcagcc tgtgattgag gaagaaatga tactcctcac
     1141 ggctcatgcc gttgtccatc agatactcgt acatctcgct gtcgacgcgg cggccgtaat
     1201 tgaggtcgag gctgaggaaa cggcgggagt tcatcagctc cgccttgtga atcgccgacg
     1261 ggctgtcggc gtggaaatat tccgagcttt ccgactggat gaagatcgcg tcgggccgcc
     1321 gcttcagtat ctcgatcatg gcgagcacgt tcgccttgac gatgtgcttc agcgcggtga
     1381 cgaagctgcg gtcgtccttg gcctgctcgt tccaccagcc gtatttcgcc gagaagatcg
     1441 cgcatatgaa catctcgttg accggcgtgt agagctgcac ccacgggaag cggtcggcga
     1501 acgcgccggc ataggtggcg aagagctgcg ggaaatcggg attctggaaa ttgccgatcc
     1561 agtccgggac gccgaaatga cataggtcga cgatgggcgt gaggttgcgc cgcttcagct
     1621 cagcgaaggt gaggtcggcg aactcccaat cataggtgtt gggaccgaga agggtgcggt
     1681 agagcggcgg cccgtagcgc aggaaatgga ttccaagctc gtcgcagcag tcgaagtcgg
     1741 tgcgccagtg ccggtaatgg ccgcacacct ccatttggtc gacccgcgtc cggccgccgt
     1801 tgatcgtcgg gatgctgttc tcgatcccgg tcgcgaaaat gaagttggcg atgaggccct
     1861 ccctcggctt cgtgcggtca aacggctagc ccgcgcgcgg cggcggggcc agcccgcaat
     1921 gcgtggagag gaggaaaaaa ggcgacgcgg cggagaggct cgcttccccc agctagagac
     1981 attcttattg taatgagact ttggtctcat tatattgcag cctactcgat tgtgggagga
     2041 ggaaagaatg aggctgagcc gagcgcagat ggacgccaag atggacgctc acttcgcctt
     2101 cgaggcccag gacgacgtgg agggcgtgct ggcgacgctg accgacgacg cggagcacga
     2161 catcgtcggc tggccaggcg ggccgacgcg gggacgggaa ggcccgcggg gcttctacga
     2221 gaccctcttc ggcgacctcg ccgacggccg ggtggaaacg ctgcggcgtt attatggcga
     2281 cgacttcatg gtcgacgaat cgctctggcg aggccgggcg cccggacggc cgttcggaat
     2341 cgagggtcga ggacgcccgc tcgaattccg ccttctccac gtcgtggagt tcgacgaggc
     2401 cggcgacatg aaacgtgaga atgtgtggct ggatctggcc gcgatccaac gccagctgca
     2461 ggacgtctga tggcgcagac cggcagcggc ggacgcccca accagcgccg ccgtacgcgc
     2521 aaggatctgc tcgaggcggc ggcccggctg atgcggcggg ggaaaagccc cacccttgac
     2581 gagatcgcgg aggaggccct ggtgtcccgc gcgaccgcct atcgctattt cccgggggtc
     2641 gaggcgctgc tgctcgaagc ttcgctcgat gtcgctttcc ccgacgcggc cgccttgttc
     2701 gccgaggggc ccctcgacgt cgcggcccgc gtcgaggccg cggaagcggc ggtgagcgag
     2761 atggtgcggg agaatgagcc cgcgctgcgt acgatgctcg ccaatgccct ccaggcgcgg
     2821 cccaaagccg agccggacga cctgcccgcg cggcagaacc gccgctcgca gctgatcgat
     2881 gcggcgctgg cacccggcgc gttcgcgccg gcggcgcggg accggctcgc caaggcgctc
     2941 gccttcttca tcggcaccga ggcgatgctc gtcggcaagg gcgtgctcca gctcgacgag
     3001 gcggaagccg ccgcggtcaa gcgctgggcc atgcgctcgc tggtgacggc ggcggcccgc
     3061 gccgattcgg atacataagg agaggcgctc gggtgcggcg ggtgatagct tcccgtcgtt
     3121 cctccggaaa gggaagtgcc gatggcgagc gcagcaccga gcctcaaggg cgaatggacc
     3181 tatcgaagct tcgccaacga gcccaagccg gtcgacggca acgcccaggc ggccctgggc
     3241 ctcatcttcg gcgagggcag ctgaccatca cccaggccga cccgagcggc ttccgggcgg
     3301 ccctgtcgtt cggcggcaag tcggtgatgg acctgatcgg cagcttccgg ccggccgggg
     3361 ccggcgcccc cgcggtcatc gccgcccgcg gcaccggccg caaggggtcc ggaatcgagg
     3421 atttcgtcta cgactacatc ttctatccgg ccccggcctg gccggccggg atcggccagc
     3481 gccaggcact ggtcggcacg gtgcttcggg ccgccgacca tggcggcgcc aagaagggcg
     3541 ccgtcgtctc cgcgatcgcg gttcggcgcg gctccaagcc ggccggctga tcgccggcgc
     3601 cggggcgacg cggcccttgg cccggtgaaa ccgctgggct cgcccgctta agggcgttaa
     3661 cgacgccaga atgggcatgc gctgggatga aggtttcatc gccgtcgact ggggcacgac
     3721 caatcgtcgc gcctggcggc tcggtcccgg gggcgccgtc gccgcggaga tggaggacga
     3781 ccagggcata ttggcggtgc cgaagggcgg gttccattcg gctgtgggcg cggtccgtgc
     3841 gcggctcggc gacctgccga tgctgatggc cggaatgatc ggctccaacc gcggctggat
     3901 cgaggcggat tacgtcccct gccccgccgg ccttccggac ctggctgcgc ggctgcgctg
     3961 gatcgacggc gagcgggccg cgatcgtgcc cggagtctcg ttcgacggcg acggcagggc
     4021 ggacgtcatg cgcggcgagg aggttcagct gctcggcgcc tatgccgaag gctggatcga
     4081 cggcgacgcc tctctctgcc accccggcac ccacaacaaa tgggtgcggc tcgccgacgg
     4141 ccgaatcgcc gccttccgga cggtgatgac cggggagatg ttcaacctgc tgcgggagca
     4201 cagcatcctg gcggacctgc tggctgcccc cgccgaaccg ggcgccgcat tcgacgccgg
     4261 cgtccggcac ggtctcgacc gcgacgatct catcgccgag ctgttctcgg tgcgcgcgcg
     4321 cgtcctgctc ggcaaggcgc cgcgcgagga ggcggccgcc tatgtcagcg ggcttctgat
     4381 cggcgccgac ctgaaggtgg gcctgaggct cgtcggcgac ggcgagatcg tcgcgatggg
     4441 ccggccggag ctgacccgcc tgttcgccgc ggcgctggcg gtggccggcc ggcctgcgcg
     4501 ggcggtcgac ggcgaggcgg cctttctcgc cggcgtgcgg catctcgcgg agcttgtgca
     4561 atgacccctg acgaagagct cggacggcgg ctgtccgaat gcccgttggt cgcgatcatc
     4621 cgcggagtca cccccggcga agccgaggcg atcggagcgg cgatcgtcga ggctggaatc
     4681 ggcatcgtcg aagtcccgct caactcgccc gacccgctcg cgagcatcga gcggctggcc
     4741 aggagccttg gcgactcggc gctgatcggc gccggcaccg tgctcgatcc gttcgacgtc
     4801 ggccgagtgc gcgacgccgg cggccggatc atcgtgtcgc ccaacacctt cccgccggtc
     4861 atcgaggcct cggcggcggc cgggctggtg tcgctgcccg gctatttcac gccgtcggaa
     4921 gccttcgccg cgctgcgcgc cggcgcgacg gcgctgaagc tgtttccggc cgagggggcg
     4981 tccccggcgg tggtgagggc acagcgggcg gtgcttccca agggggtgcc gctgctcgtc
     5041 gtcggcggca tcgcccccga caatatgggc ccgtggctgg aggcgggtgc gagcggtttc
     5101 ggcctcggct cgggcctcta caagcccggc cagtcggccg aggagacggc ggcgaaggcg
     5161 cgcgcttatg tcgaagctgt gcagcgctca gcctagagcc gcctccgcct tggcgaaggc
     5221 tgcctcgtcg tcgacgtcga gccaccagc
//
LOCUS       DASVQZ010000104         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_310927, whole genome shotgun sequence.
ACCESSION   DASVQZ010000104 DASVQZ010000000
VERSION     DASVQZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310927"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..524)
                     /locus_tag="VF547_02875"
     CDS             complement(78..524)
                     /locus_tag="VF547_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8261798.1"
                     /translation="MSFVSARDNPHVAALREAYRRWRDTRGGCADEILALFDDRIEMR
                     SVLAAEDVPSQLAGVHRTRAEAAEYFAALARDWEMVDYVIEQFVADGEYGEDVVAIGW
                     CAWRHRASGTVVETPKVDIWRFQFGKATRFTEMFDSLAFARAVGAA"
     gene            complement(602..1453)
                     /locus_tag="VF547_02880"
     CDS             complement(602..1453)
                     /locus_tag="VF547_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8261799.1"
                     /translation="MDRPPLRRTASRPFPLLALLAAACSSPAPAPEEQPSAQAAPAFA
                     RLSADPVAHGERLGTVLGCNGCHGKDLTGEDWSDPEWGRLWTANLTRAVPRFADDSAL
                     DRAIRGGVGHDGRELWGMPSSLFTHLADSEVKALIAWLRSHRPAGSEHPLPTFGPRAR
                     KEIAAGTWRSSAAEVRQDRGIWPPDAGPGHALGRYIVRATCAECHGLRLEGDKDQEKD
                     RPAPPLAVAAAYDRDQFRHLLRTGKPIGGRELRMMSAVARWRYSRFTEGELDSVHAYL
                     KAVADRR"
     gene            1669..2865
                     /locus_tag="VF547_02885"
     CDS             1669..2865
                     /locus_tag="VF547_02885"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8261800.1"
                     /translation="MRRSFKAMLRNARLRVWLFVFPALAILTGTLAALILNGHEATGQ
                     RREADRRYVQALDVLLATSEIKSAVNAAIRGERGYLLTNDDRFLRPYAESRRDIPRLY
                     DRLRRLVRDNPGQSAEAERLRGRLTSYYALLDHTIALAAAGRGEAAQRVVRRGLGKRE
                     VENVLRAVGRIEAEEHRLLARRRAAFALADQRSESYGLALAAAGLAMLILLVVSAIAA
                     RRANARAAEATAELQRIAATDALTGLWNRRHLLERMEAEIGRSRRAGRPLCLAILDVD
                     HFKRVNDTHGHPAGDEVLRVLARRVRDAVRSSDVVGRMGGEEFAILMPETDRAQAQLV
                     CERLRETVAARPVRLPSGADLAVTLSTGVALMAGGEAPDRLISRADSALYDAKAEGRN
                     CVRLAA"
BASE COUNT          610 a         1364 c         1476 g          566 t
ORIGIN      
        1 tcgacggccg ccaatgaggg cgatcggccc gtgatggccg gctgacccca tcctcgtgcg
       61 cgcgcggcgc gccggcttca ggccgcgccg accgcgcggg cgaaggcgag gctgtcgaac
      121 atctcggtga agcgggtcgc cttgccgaac tggaagcgcc agatgtcgac cttgggcgtc
      181 tcgaccaccg tgccgctggc gcggtggcgc caggcgcacc agccgatcgc caccacgtcc
      241 tcgccatatt cgccgtcggc gacgaactgc tcgatgacgt agtcgaccat ctcccagtcg
      301 cgggcgaggg cggcgaaata ttcggcggcc tcggcgcggg tgcggtggac tccggcgagc
      361 tgggagggga cgtcctccgc ggcgagcacc gagcgcatct cgatccggtc gtcgaaaagg
      421 gcgaggatct cgtcggcgca gccgcccctc gtgtcgcgcc agcgcctgta cgcctcgcga
      481 agcgccgcga cgtgcggatt gtcccgggca gacacgaagc tcactccccc accgctggcc
      541 ccggggcgca gcgtagcaga aggcggcccg agggtcgagg gcgggggcgc gggggccgtg
      601 gtcagcgccg gtccgcgacg gccttcagat aggcgtggac ggaatcgagc tcgccctcgg
      661 tgaagcgcga atagcgccag cgggcgaccg cgctcatcat cctgagctcg cggccgccga
      721 tcggcttgcc ggtgcgcagg agatgccgga actggtcgcg gtcgtaggcg gcggcgaccg
      781 cgagcggcgg cgcgggccgg tccttctcct ggtccttgtc gccctcgagt cggagcccgt
      841 ggcattcggc gcaggtggct cggacgatgt agcggccgag cgcgtggccc gggccggcgt
      901 cgggcggcca gatgccgcga tcctggcgga cctccgcggc ggaggagcgc caggtgccgg
      961 ccgcgatctc cttcctggcg cgcgggccga aagtggggag cggatgctcg cttccggcgg
     1021 ggcggtggct ccgcagccag gcgatcagag ccttcacctc ggaatcggcg agatgggtga
     1081 agaggctcga gggcattccc cacagctcgc gcccgtcatg gccgacgccg ccgcggatcg
     1141 cccggtcgag cgcggaatcg tcggcaaagc gggggacggc ccgggtgagg ttggcggtcc
     1201 acagccggcc ccattcggga tcgctccagt cctcgccggt gagatccttg ccgtggcagc
     1261 cgttgcagcc gagcaccgtg ccgagccgct cgccatgggc gacgggatcg gcggagagcc
     1321 gggcgaaggc gggcgcggcc tgggcggagg gctgctcctc gggcgcggga gcgggcgagg
     1381 agcaggcggc ggcgaggagg gccaggagcg gaaacggccg cgaggcggtg cggcggaggg
     1441 gcggacgatc catcggacgg ctccctgagg cggtgcgcgg atcggcgaca ctatagattt
     1501 tcgccgggct tgcgagcctc gccctccggg aaagccgatc ccggctgggc ctgcggcggg
     1561 ccgacggacg gaaggttccg aagccggccg aaggacacgc cttgccgctt tcttaaccgg
     1621 cttccggcta ggcttcccgc tcggaccttc ccgcggccga ggaccaggat gcgacgcagt
     1681 ttcaaggcca tgctccgcaa cgcacggctg cgggtctggc tgttcgtctt cccggcgctc
     1741 gccatcctca ccggcaccct cgcggcgctg atcctgaacg gccacgaggc gaccgggcag
     1801 aggcgcgagg ccgaccgccg ctacgtccag gcgctcgacg tgctgctcgc gaccagcgag
     1861 atcaagagcg cggtcaacgc cgcgatccgc ggcgagcgcg gctatctgct gaccaacgac
     1921 gaccggttcc tgaggcccta tgccgagagc cgccgcgaca tcccccggct ctacgatcgg
     1981 ctccgccgcc tcgtccgcga caatccgggc cagagcgccg aagccgagcg gctgcgcggg
     2041 cggctgacca gctattacgc gctcctcgac cacaccatcg ccctggcggc ggcggggcgg
     2101 ggcgaggcgg cccagagggt cgtccgccgc ggcctcggca agcgcgaggt cgagaacgtg
     2161 ctgcgcgcgg tcggccggat cgaggccgag gagcatcgcc tgctcgcccg ccgccgggcc
     2221 gccttcgcgc tcgccgacca gcgctccgaa agctacggcc tggcgctggc ggcggcgggc
     2281 ctggcgatgc tgatcctgct cgtcgtctcg gcgatcgcgg cgcgtcgggc gaacgcccgg
     2341 gcggccgagg cgaccgccga gctccagcgg atcgccgcca ccgacgccct caccggcctg
     2401 tggaaccggc gccacctgct cgagcggatg gaggcggaga tcggccggtc gcgccgcgcc
     2461 ggccggcccc tgtgcctcgc catcctcgac gtcgaccatt tcaagcgggt caacgacacc
     2521 cacggccacc ccgccggcga cgaggtgctg agggtgctgg cgaggcgggt cagggacgcg
     2581 gtgcggtcga gcgacgtcgt cgggcggatg ggcggcgagg agttcgccat cctcatgccg
     2641 gagaccgacc gggcccaggc gcagctggtc tgcgagcggc ttcgcgagac ggtggcggcg
     2701 aggccggtgc ggctgccttc cggggcggac ctggcggtga ccttgagcac cggggtcgcg
     2761 ctgatggcgg gcggggaggc gccggaccgg ctgatctccc gcgcggactc ggcgctctac
     2821 gacgccaagg cggaggggcg gaactgcgtc aggctcgccg cctgaggcgt ccgcggcggc
     2881 ttccggccag gaccccggcg cctggaaacg cggccttttc ccggcaaagt tcatgaagtt
     2941 tatcgagtcg ggcgcctttt gaatgaactt cgagccgaga atcatggccc gccaaggcct
     3001 tggccccgcc gcccccggga ttcccaccgg ccgttcccgg cccatcaccg accccctccg
     3061 ttccgccgcg gccccgcagg cttgagcagc gcctgtcgca acgctggaac atagcagaaa
     3121 cgcgccgtcc gcgatagctc gggcagccga ggggatggag cgacgttcgc ttcgcacgaa
     3181 ggcacgaagg gcacgaaggc acgaaggcgc cgtggggcgg cggggcggag cgttctcccg
     3241 cagctggcgc cagaaagagt gacagtatac attttagcgc caggcacagc cgggccacgc
     3301 tccgcaaaaa ggtatactgt cactgttttt ctcaggcctc ttcggtgctt cggtgcttcg
     3361 ggcgagcgga ttcaagtcgc ggaagcggcg aggcggtgag gccagaaaga gtgacagtat
     3421 acattttagc gcctgccata gccggccgat gctttgggaa aatggtatac tgtcactgtt
     3481 ttccatgacg aaccagcgga gattcgtcct tcgacaaggc gcttcgactg cgctcagcgc
     3541 cgctcaggat gagcggaaat aaggaagcgc ctagtagcgt ccttcgacta agctcaggac
     3601 aggctcctct cccggctggc gggatcgggc gcgacagctt atgctgcccg tcagccatcc
     3661 ttcgcctcct tttatcggga gccgttcgct ggtcgcggcc gaagcgaggc gggcgccttg
     3721 gttcggccgg tcttattatt cgtgcacgat aaacaggggc gggtcaatag tcgatctcga
     3781 aaaaagaccc cgcggccgcc cgcggaccgg cgagtccccg acgaagggcg tccgcatccc
     3841 cgtcgagctg gaagcgaaga tcaccgcctg gatcgagcgg cagcctgagc ccaaaccttc
     3901 ccgctccgaa gcgatccggc ggctgatcga gagggggctg cccgcgaagt agccctggcc
     3961 ggcggaattc gaccaaggga tactaatgcc taaataaata ggagagtcgg gctctc
//
LOCUS       DASVQZ010000105         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_120600, whole genome shotgun sequence.
ACCESSION   DASVQZ010000105 DASVQZ010000000
VERSION     DASVQZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120600"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..>2153
                     /locus_tag="VF547_02890"
     CDS             350..>2153
                     /locus_tag="VF547_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX8261801.1"
                     /translation="MAVRLVPETATPAAGSKVTLALVMTPREGWHGYWRNPGDAGVGT
                     RIAWRLPEGASAGPIRFPVPGRLIIQGLMNYVYEGEHALLVDLAVPAGLAPGTKLPVR
                     GKVDYLVCTDKICVPESAEIAADLAVGPGGPKAAGFERYRRALPKPLSDPGLFERRGG
                     MLRLAISLPAAAAAQDPYFFPVTEGALVYSAPQSVSRRGDRLIVETRAGDSAPAVLEG
                     VLKLGPGLGLAVRAEPGPVGPAGLAVEPPAAASSGGRTLLLALAGALLGGLLLNVMPC
                     VFPILSLKALSLARAGGDERAARREAFAYAAGAVLACLALGAVLLAVRAGGAAVGWAF
                     QLQDPRVILFLLLLVTAIALNLAGLFELPALGGRLAGGRTGAFWTGALAAFVATPCTG
                     PFMGAALGAALILPAAAALAVFAGLGVGLALPFLLLGYVPALRRRLPRPGRWMARFRR
                     ILAVPMALTALGLAWILERQVGLAGLAVGHAAAAALALLLGWAGRRQGWWPVAPAALA
                     AGLAIAFLPAAPAPAEARSGGSLRAEPFSEARLAELRASGRPVFVYFTADWCLTCKVN
                     EAAVLDQDEVAADFRGRGVAVLVGDWTRGDAAIGR"
BASE COUNT          208 a          830 c          773 g          342 t
ORIGIN      
        1 agtatttcgg agtggcgagg atcgggcgcg accagctcga ggattatgcg gcccggcgcg
       61 gggtgagcct ccaggaggcc gagcgctggc tgagaccgaa cctcgactga taatcctccc
      121 catagaagat ggggaggggg accatgcgaa gcatggtgga ggggtagcgg cgcaggcttc
      181 tgccatccgc ccctccacca cccgtcgggt ggtccccctc cccacgcgct tcgctcgcgg
      241 ggaggataag ggatgctata cccccctcgt gaatcgcctc ctcctcgccc tcctcctgct
      301 cctctgcgcc tcgcctgcgc acgcccagct gccgccgggc cagaaggcga tggccgtccg
      361 cctcgtgccc gagaccgcga ctccggccgc cggctcgaag gtcaccctcg ccctcgtcat
      421 gaccccccgc gagggctggc acggctattg gcggaatccg ggcgacgccg gggtcggcac
      481 ccgcatcgcc tggcggctgc cggaaggggc gtccgcgggg ccgatccgct tcccggtgcc
      541 cggaaggctg atcatccagg ggctgatgaa ctatgtctac gagggggagc atgccctgct
      601 cgtcgacctt gcggtgccgg ccggcctcgc gccgggcacc aagctgccgg tgcgcggcaa
      661 ggtcgattat ctcgtctgca ccgacaagat ctgcgttccg gaatcggccg agatcgccgc
      721 cgacctcgcc gtcggccctg gcggaccgaa agccgccggg ttcgagcgct accggcgggc
      781 gctgccgaag cccctgtccg atcccgggct tttcgagcgg cgcggcggga tgttgcgcct
      841 cgccatatcg ctcccggccg ccgcggccgc gcaagacccc tatttcttcc cggtcacgga
      901 gggcgcgctg gtctattcgg cgccgcagtc ggtctcgcgc cgcggcgacc ggctgatcgt
      961 ggagacccgg gccggcgatt cggcgcccgc ggtcctcgag ggcgtgctga agctcggccc
     1021 cggcctcggc ctggcggttc gggcggagcc cgggccggtc ggcccggccg gactagccgt
     1081 cgagccgccc gcggccgctt ccagtggcgg ccggacgctt ctgctcgcgc tggccggagc
     1141 cctcctcggg ggcctgctgc tcaacgtcat gccttgcgtc ttccccatcc tcagcctgaa
     1201 ggccctgagc ctcgcccgcg ccggcggcga cgagcgggcg gcgcggcgcg aagcgttcgc
     1261 ctatgccgcg ggcgccgtcc tcgcctgcct ggcgctcggc gcggtgcttc tggcggttcg
     1321 cgccggcggc gccgcggtcg gctgggcgtt ccagctccag gatccgcgcg tcatcctctt
     1381 cctgctgctg ctcgtcaccg cgatcgccct caacctcgcc ggcctgttcg agctgccggc
     1441 gctgggcggc cggctggccg gcggccggac cggcgccttc tggaccgggg ctctcgccgc
     1501 cttcgtcgcg actccgtgca ccggtccgtt catgggcgcc gccctcggcg ccgcgctgat
     1561 attgccggcg gcggccgcgc tggcggtgtt cgccggcctc ggcgtcggac tggcgctgcc
     1621 tttcctcctg ctcggctacg tccccgccct gcggcggcga ttgccgaggc cggggcggtg
     1681 gatggcgcgc ttccgccgca tcctggcggt gcccatggct ctgaccgcgc tcggcctcgc
     1741 ctggatcctg gagcgccagg tcggcctcgc cggcctcgcc gtcggccatg ccgccgcggc
     1801 cgccctggcg ctgctcctcg gctgggcggg ccgccggcag ggctggtggc cggtggcccc
     1861 cgcggctctc gccgccggcc tggcgatcgc cttccttccc gccgctccgg cgccggccga
     1921 ggcccgctcc ggcggctcgc tccgcgccga gccgttcagc gaggcccgcc tcgccgagct
     1981 gcgcgcatcg ggccgcccgg tcttcgtcta tttcacggcc gactggtgcc tcacctgcaa
     2041 ggtcaacgag gccgcggtgc tcgaccagga cgaggtcgct gccgacttcc gcggccgcgg
     2101 cgtcgcggtc ctggttgggg actggacccg cggcgatgcg gcgatcgggc ggt
//
LOCUS       DASVQZ010000106         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_599035, whole genome shotgun sequence.
ACCESSION   DASVQZ010000106 DASVQZ010000000
VERSION     DASVQZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599035"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..924)
                     /locus_tag="VF547_02895"
     CDS             complement(163..924)
                     /locus_tag="VF547_02895"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8261802.1"
                     /translation="MLESVSAVVREAGAIAAARCGTAFRRWEKAPGHPVCDIDLEVDG
                     FLRERLSAIDPEAGWLSEETLDSSDRIERPRVWVVDPIDGTRDFLRGRPGWCVSVALV
                     EDRTPLIGVLDAPARGEHWTARKGGGAWRNGSRLRVSGRRDLAGARVPAEQLADPDRD
                     LVAVSRPNSIALRLALVAAGEADLAATLRWGFEWDIAAAALIAAEAGATVSGALGQPL
                     AFNSANGEAFGVLAATPAIHAAAVERLRARAMDLV"
     gene            complement(917..>1739)
                     /locus_tag="VF547_02900"
     CDS             complement(917..>1739)
                     /locus_tag="VF547_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX8261803.1"
                     /translation="GYSSSASLIAGAGSEMQRDYAYHSVRHYAQLEAPETIGRRAAER
                     AVARLRPARLASGTMPVLFDPRVGDSLLGHLTAAISGPSITRRTSFLLDKEGDMVFPQ
                     GVTVRDDPHRPRGLRSKPFDGEGLPTRPSNLIEAGRLTGWLLDSASARQLGRGPTGHA
                     VRGSSGGPNVGATNVHMEPGPLSPAQLIGEVERGVYVTQLIGQGVNPVTGDYSRGASG
                     FIVEKGRLGAAVEEITIAGNLIDMFANLVPADDLRFRRAVNVPTLRIDGMTVAGA"
BASE COUNT          234 a          655 c          632 g          218 t
ORIGIN      
        1 cgcggtcccc ctccccggca aaggccgggg aggaatggag gagaccagga ttcctccgtt
       61 cgggctgagc tcgtcgaagc ccgttccttc tcccgcagcg cgcaacggcg aggccttcga
      121 caggttcggg ccgaacgggg atgtcgcgac gccgcgcccg gctcagacca ggtccatcgc
      181 ccgcgcccga agccgctcga ccgcggcggc gtggatggcg ggcgtggcgg cgagcactcc
      241 gaacgcctcg ccgttggcgc tgttgaaggc gagcggctgt cccagcgccc cgctcaccgt
      301 cgccccggcc tcggcggcga tcagggcggc ggcggcgatg tcccactcga agccccagcg
      361 cagggtcgcc gccagatcgg cctcgcccgc cgccaccagc gcgaggcgca gggcgatcga
      421 gttgggcctc gacaccgcga cgaggtcgcg gtcggggtcc gcaagctgct cggccgggac
      481 ccgcgcgccg gccaggtccc ttcggccgct gacgcgaagc cgcgagccgt tgcgccaggc
      541 gccgccgccc ttccgcgccg tccaatgctc gccgcgcgcc ggagcgtcga gaacgccgat
      601 cagcggcgtc cggtcctcca ccagcgccac cgacacgcac cagccgggac ggccgcgcag
      661 aaagtcgcgc gtgccgtcga tcggatcgac cacccacacc cgcggccgct cgatccggtc
      721 ggaggaatcg agcgtctcct ccgaaagcca gccggcctcg ggatcgatcg cgctgaggcg
      781 ctcgcgcagg aagccgtcga cctcgaggtc gatgtcgcac accggatggc cgggcgcctt
      841 ctcccagcgc cggaaggcgg tgccgcagcg ggcggcggcg atcgcccccg cctcgcggac
      901 gaccgccgag accgactcaa gcaccggcca ccgtcatccc gtcgatcctc agcgtcggga
      961 cgttgaccgc ccggcggaag cgcaggtcgt ccgcggggac gaggttggcg aacatgtcga
     1021 tcagattgcc cgcgatcgtg atctcctcga ccgcggcgcc tagccggccc ttctcgacga
     1081 tgaagcccga cgcgccgcgg ctgtagtcgc cggtcaccgg gttgacgccc tggccgatca
     1141 gctgggtgac gtagactccg cgctcgacct cgccgatcag ctgcgccggg ctcagcggcc
     1201 ccggctccat atggacgttg gtggcgccga cgttcggccc gccggaggag ccgcggacgg
     1261 cgtggccggt cggaccgcgc ccgagctgcc gcgccgacgc gctgtcgagc agccagccgg
     1321 tcaggcggcc cgcctcgatc aggttggacg gccgggtcgg cagcccttcc ccgtcgaacg
     1381 gcttggagcg caggccgcgg ggccgatgcg ggtcgtcgcg gacggtgact ccctgcggga
     1441 acaccatgtc cccctccttg tcgagcagga agctggtgcg ccgggtgatc gaggggccgc
     1501 tgatcgcggc ggtcaggtgg ccgagcagcg agtcgccgac ccgcgggtcg aacagcacgg
     1561 gcatcgtccc gctcgccagc ctggccggcc gcagccgggc caccgcgcgc tccgcggcgc
     1621 ggcggccgat cgtctcgggg gcttcgagct gcgcgtaatg gcgaacgctg tgataggcgt
     1681 agtcgcgctg catctcgctg ccggcgccgg cgatcaggct cgccgacgag gaatagccg
//
LOCUS       DASVQZ010000107         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_599508, whole genome shotgun sequence.
ACCESSION   DASVQZ010000107 DASVQZ010000000
VERSION     DASVQZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599508"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VF547_02905"
     CDS             <1..1171
                     /locus_tag="VF547_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261804.1"
                     /translation="AVHTTLYDLANIFLIGGILLFPHGRLSPRITLALLAALPLLLFL
                     SGDFYRSFLIAAMAASVLLFLWRLRTAAGDERQQLKWAVFGFSGYAFFLGVSLTTDML
                     KAGAASLGQQLALELAAGFAFGLAFLLLQAGLLVALLRYRLYDAEVAISRSASFALIA
                     LILAGIFAATMEGVKEVILRFFGRDAGSIAPIVGAALSTVMINPVYERVQAWAERRFQ
                     RRLAEMRRDLPDCLRDLRHFASLSELVEEVLARISAGVRSARLALVLDGRTTGTRGVG
                     AEAVDAWLAATALDPSCSSGVETGDPFFPLRRPLSLDDGTCLGWILLGPHPDRSRLST
                     AELDALDELAEPVARAVRVVLAREARERALAETLARQQRQIDALARRLPREAGNN"
     gene            1269..1964
                     /locus_tag="VF547_02910"
     CDS             1269..1964
                     /locus_tag="VF547_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261805.1"
                     /translation="MAGTAFKALIGAASAAMLAAPAALAAAPLVVRSLGPSAKAYPPG
                     RAVGENVPLVLKAGDVVTVLAPSGARTLRGPGTFKVAGGGTAPPFNPRSRFGAMRGDE
                     KPPVPGLFDVDVSRGGTMCLADATKVLLWRPTSAADATLELVPAGGAAQRLDWAAGSL
                     MQRWPQQLPIADGAEYSVKFADEAQARRLRFKTLAAAQDDPTALAKALIDNGCQAQLD
                     LLIQNAPQEADGG"
     gene            2033..2356
                     /locus_tag="VF547_02915"
     CDS             2033..2356
                     /locus_tag="VF547_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261806.1"
                     /translation="MPDLPSAYPLQDLFDAAVRGRSVRLTCRECRHSNVFSSHALWWL
                     FHRKGWRDGFADVRRRCVCLLCLHRRRRKVRNPELELGADPPTDATLPLPPEMDWKRE
                     LRRRR"
     gene            complement(2377..>2706)
                     /locus_tag="VF547_02920"
     CDS             complement(2377..>2706)
                     /locus_tag="VF547_02920"
                     /inference="COORDINATES: protein motif:HMM:NF025460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HEX8261807.1"
                     /translation="EAALPLLWAVGLADDLKRPDAQSEPFDILKPLVHFRRHELLERA
                     ALRPAGELLDSADLHFCWECASRLALEEEAPPPAGLDGGVLLERHRAFTWLVQGGGDW
                     DEAAARA"
BASE COUNT          329 a         1032 c          927 g          418 t
ORIGIN      
        1 cgcggtccac accacgctct acgacctcgc caacatcttc ctgatcggcg ggatcctgct
       61 cttcccgcac ggccggcttt cgcctcggat cacgctcgcc cttctcgccg ctttgccgct
      121 cctcctgttc ctcagcggcg atttctaccg ctccttcctg atcgcggcga tggcggcgtc
      181 cgtcctcctc ttcctgtggc ggcttaggac cgccgccggc gacgagcgcc agcagctcaa
      241 atgggcggtg ttcggctttt ccggctacgc cttcttcctc ggggtctcgc tgaccaccga
      301 catgctcaag gccggcgcgg cgagcctcgg ccagcagctc gcgctcgagc tcgccgccgg
      361 cttcgccttc ggcctcgcct tcctgctcct ccaggccggc cttctcgtcg ccctgctccg
      421 ctaccggctc tacgacgccg aggtggcgat cagccgctcg gcgagcttcg cgctgatcgc
      481 cctcatcctc gccggaatct tcgcggcgac gatggagggg gtgaaggagg tgatcctgcg
      541 cttcttcggt cgtgacgccg gctcgatcgc tccgatcgtc ggcgccgcct tgtcgacggt
      601 gatgatcaac ccggtctacg agcgcgtcca ggcctgggcc gagcggcgct tccagcgccg
      661 gctcgccgag atgcgccgcg acctgcccga ctgcctgcgc gacctgcgcc atttcgccag
      721 cctctccgag ctcgtcgagg aggtgctggc ccggatctcg gccggggtgc gctcggcccg
      781 gctggcgctg gtgctggacg ggcggacgac ggggacccgg ggcgtgggcg ccgaggcggt
      841 cgacgcctgg ctcgccgcca ccgcgctcga tccgtcctgc tcgtcgggag tggagaccgg
      901 cgatcccttc ttcccgcttc ggcgcccgct cagcctcgac gacggcacct gcctcggctg
      961 gatcctgctc ggcccgcacc ccgaccggtc ccggctcagc accgcagagc tcgacgcgct
     1021 cgacgagctc gccgagccgg tcgcccgagc ggttcgggtg gtgctggcgc gcgaggcgcg
     1081 cgagcgcgcc ctggccgaga cgctcgcccg tcagcagcgg cagatcgacg cgctggccag
     1141 gcgcctcccg cgcgaggccg gcaacaattg acacgaagcc gcgccggtcg cggcacgaag
     1201 ctgaaacagg gctggattag cccgccgttc aaaggttatc ttgggatgtc cggcgaaagg
     1261 tggaagacat ggcgggaacc gcgttcaagg cgttgatcgg agcggcctcg gccgcgatgc
     1321 tggcggcgcc cgccgcgctc gccgccgcgc cgctcgtcgt gcgctcgctg gggccgtcgg
     1381 ccaaggccta tccgcccggc cgcgcggtcg gcgagaatgt gcccctggtg ctcaaggccg
     1441 gcgacgtggt caccgtgctt gctccgtcgg gcgcgcgcac cctccgcggc ccgggcacgt
     1501 tcaaggtcgc cggcggcggc accgccccgc ccttcaaccc ccgctcgcgc ttcggcgcga
     1561 tgcgcggcga cgagaagccg cccgtgccgg gcctcttcga cgtcgacgtg tcccgcggcg
     1621 gcaccatgtg cctggccgac gccaccaagg tcctgctctg gcggccgacc agcgccgccg
     1681 acgcgaccct ggagctggtt cccgccggcg gcgccgccca gcgcctcgac tgggcggccg
     1741 gctcgctgat gcagcgctgg ccgcagcagc tgccgatcgc cgacggggcc gaatattcgg
     1801 tcaagttcgc cgacgaggcg caggcccggc ggctgcgctt caagaccctc gccgccgccc
     1861 aggacgatcc gaccgccctc gccaaggcgc tgatcgacaa tggctgccag gcccagctcg
     1921 acctgctcat ccagaacgcc ccccaggaag ccgacggcgg ctaggcggga tcgcgccgcc
     1981 gaaatcgcgg acggggcttt cgaagcccgt ctatgttcgc tatttgttcc ggatgccgga
     2041 tcttccttcc gcctatccgc tccaggatct gttcgatgcg gccgtgcgcg gccggtcggt
     2101 tcggctgacc tgccgcgagt gccgccacag caacgtcttc agcagccacg ccttgtggtg
     2161 gctgttccat cgcaagggct ggcgggacgg gttcgccgac gtccggcggc gctgcgtctg
     2221 cctgctctgc ctccaccgcc ggcggcgcaa ggtgcgcaat cccgagctgg agctgggcgc
     2281 ggacccgccg accgacgcca ccttgcccct gccccctgaa atggactgga agcgcgagct
     2341 tcggcgccgc cgctgagccg ggaagggtgc ggcgcttcag gcccgcgccg ccgcttcgtc
     2401 ccagtcgccg ccgccctgga ccagccaggt gaaggcgcgg tggcgctcga gcaggactcc
     2461 cccgtcgagg cccgcgggcg gcggcgcctc ctcctcgagg gcgaggcggg acgcgcattc
     2521 ccagcagaaa tggaggtcgg cggaatcgag cagctcgccc gccggccgaa gcgccgcgcg
     2581 ctccagcaac tcgtggcggc ggaaatggac gagcggcttc aggatgtcga acggctcgct
     2641 ctgggcgtcg ggccgcttca ggtcgtcggc caggccgacc gcccacagca agggcagggc
     2701 cgcctc
//
LOCUS       DASVQZ010000108         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_218756, whole genome shotgun sequence.
ACCESSION   DASVQZ010000108 DASVQZ010000000
VERSION     DASVQZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218756"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1020)
                     /locus_tag="VF547_02925"
     CDS             complement(67..1020)
                     /locus_tag="VF547_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4424 domain-containing protein"
                     /protein_id="HEX8261808.1"
                     /translation="MKRYLILLIALAAPAAANDSTAEHAAGGLVLTRSADIDMVSEDL
                     FVSARLVRVRYVFRNRSPRDIRTTVAFPMPDRDLGEANFSDVAYPEDFRTTVDGRPVT
                     MAVERKALLKGADRTALLAGLGLAPESEGKALDALGEAQRERLVKLGLAAIDEYDNGK
                     GWEKHYVPAWTVKETWHWQQVFPAGRDLVVEHSYVPGTGGSVDAALGFPEFRAGPEGR
                     AMIRDYCVEPDFLAGLDRLRRRSPATPEQRIGYILTTGGNWRSPIGRFRLVVDKGAPE
                     NLVSFCGEGVRKISPTRFEMVRSNWRPERDLKVLIVTPGGE"
     gene            complement(1138..1407)
                     /locus_tag="VF547_02930"
     CDS             complement(1138..1407)
                     /locus_tag="VF547_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEX8261809.1"
                     /translation="MTDRDFLLQVQGYGLMTAEVAYYMPDHPKLIQLFVWQQYDVAPR
                     FPVLHRFLDHWRTDVEAVLHSVRVAHQHLIRPAEWRAVDGVISIN"
     gene            1645..>2221
                     /locus_tag="VF547_02935"
     CDS             1645..>2221
                     /locus_tag="VF547_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261810.1"
                     /translation="MFRILLLAAAIAGLSWFQFVNTASARDVGRNAADLFDPRPARDI
                     LFVGNSRMFTNDLPSMVRAVADSADSPVKYRVRMWALPGRTFVDHARSKAVQALLAER
                     WDRVILQAQSAAQLEQGSRADFTAYGGRLIGRARAAGSPSSLVVNWTYSEAVFREHPG
                     WTRSEAYALIQRDHRALARTSGAELINVGTMF"
BASE COUNT          350 a          748 c          774 g          349 t
ORIGIN      
        1 ccggaagaac ccctccaccg ggcttcgcct ggtccccctc cccggcaaag gccggggagg
       61 aattgatcat tcgccgcctg gcgtcacgat cagcaccttg aggtcgcgct cgggcctcca
      121 gttcgaccgg accatctcga accgcgtcgg cgagatcttg cgcacgccct cgccgcagaa
      181 gctgaccaga ttctccggcg cgcccttgtc cacgacgagg cggaaccggc cgatcggcga
      241 acgccagttg ccgccggtgg tgaggatgta gccgatccgc tgctccggag ttgccgggct
      301 ccggcgccgc agccggtcga ggccggcgag gaagtcgggc tcgacgcaat agtcgcggat
      361 catggcgcga ccttcggggc cggcgcggaa ttcgggaaag ccgagcgccg cgtcgacgct
      421 gccgccggtg cccggcacgt agctgtgctc gacgacgagg tcgcgtccgg ccgggaagac
      481 ctgctgccag tgccaggtct ccttgaccgt ccaggcgggg acgtaatgct tctcccagcc
      541 cttgccattg tcatattcgt cgattgcggc gaggccgagc ttcaccagac gctcgcgttg
      601 cgcctcgccg agggcgtcca aggctttccc ctccgattcc ggggcgaggc cgagcccggc
      661 gaggagggcg gttcggtcgg cgcctttcag gagcgccttg cgctccaccg ccatcgtcac
      721 cggccgcccg tcgaccgtcg tccggaaatc ctcgggatag gcgacgtcgc tgaaattcgc
      781 ttcgccgagg tctcggtcgg gcatcgggaa ggcgaccgtc gtgcggatgt cgcggggcga
      841 gcggttgcgg aagacgtagc gcacgcgcac cagccgggcc gagacgaaca ggtcctccga
      901 gaccatgtcg atgtcggcgc ttcgggtgag caccaggccg ccggcggcat gctccgcggt
      961 gctgtcgttg gcggcggccg gcgcggcgag cgcgatgagg agaatgaggt atcgcttcat
     1021 cctcgaagct tatcacaagt gtccctcccg tcaccccggc ggaggccggg gtcccccgag
     1081 aagaaggcac tgccaagtgg acggggatcc cggccttcgc cgggatgacg ctgcgcgtca
     1141 gttgatcgag atcaccccgt ccacggcgcg ccactcggcg gggcggatga gatgctggtg
     1201 ggcgacccgc acggagtgga gcaccgcttc gacgtcggtg cgccaatggt ccaggaagcg
     1261 gtggaggacg gggaagcgcg gcgcgacgtc atattgctgc cagacgaaca gctggatgag
     1321 cttgggatgg tccggcatgt aataggcgac ctcggccgtc atcagcccgt agccctggac
     1381 ctggagcagg aaatcgcggt cggtcatcgc gccctccgaa gcttgcgggg gccagcatag
     1441 gaaaagatgc ttaacgggac gctaaccatg atcggccttc ctccaacgac ggcggcggcc
     1501 ccgccgtctc tcaatggaat cgtaaatcgc gcgtcgccgc aagccgttcc gccgagcgcg
     1561 ggggacgtcg ccggccgagg ctcggcttaa cccgctgcta gggaaacgcc tttaggcaca
     1621 ggcgctcatc ggcgagggcg cgcaatgttt cgaatcctgt tgctggcggc cgcgatcgcc
     1681 ggcctgtcct ggttccagtt cgtgaacacg gcctcagccc gcgacgtcgg ccgcaacgcc
     1741 gccgacctgt tcgatccgcg gcccgcccgc gacatcctgt tcgtcggcaa cagccgcatg
     1801 ttcaccaacg atctgccctc gatggtccgt gccgtcgccg attcggccga ctccccggtc
     1861 aaatatcggg tgaggatgtg ggcgctgccc gggcggacct tcgtcgacca tgcccgaagc
     1921 aaggccgtcc aggcgcttct ggcggagcgt tgggaccgag tgatcctgca ggcccagagc
     1981 gccgcccagc tggaacaggg cagccgggcc gacttcaccg cttacggggg gcggctcatc
     2041 ggccgggcgc gagcggcggg ctcgccgtcg agcctggtcg tgaactggac gtacagcgag
     2101 gcggtgttcc gcgagcatcc gggctggacc cgcagcgagg cctatgccct catccagcgc
     2161 gatcaccggg cgctcgctcg gacgagcgga gcggagctga tcaacgtcgg caccatgttc
     2221 a
//
LOCUS       DASVQZ010000109        12846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_408634, whole genome shotgun sequence.
ACCESSION   DASVQZ010000109 DASVQZ010000000
VERSION     DASVQZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408634"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF547_02940"
     CDS             complement(<1..192)
                     /locus_tag="VF547_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8261811.1"
                     /translation="MAMNVGQESETGEESPMSDINTTPLVDVMLVLLIIFLITVPVVI
                     QTIPMKLPKVDFEPTQTKPE"
     gene            complement(270..1109)
                     /locus_tag="VF547_02945"
     CDS             complement(270..1109)
                     /locus_tag="VF547_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8261812.1"
                     /translation="MATPAAGAAHGGDNPYGLMAALEQGGAIAWATFIILVIMSAGSW
                     YVLFVKLFEQQKIINQGRRARTSFWQSANLREGASKLEKNSAYRQIVDDGILALEQHD
                     KLTDPIDQHDWMLNSLARSQGAISSRLSSGLAFLATVGSTAPFVGLFGTVVGIYRALV
                     KIGAAGQASIDTVAGPVGEALIMTALGLVVAVPAVLAYNWLIRRNKAIVEELARFSNE
                     LHGYMMSGGAVRPVIASRAGGAAGGAAAGAGAAKGPGAASPGGTTATGSAVGAPHGGP
                     KKV"
     gene            complement(1168..1857)
                     /locus_tag="VF547_02950"
     CDS             complement(1168..1857)
                     /locus_tag="VF547_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8261813.1"
                     /translation="MAYADNRMSKSRMSAIFIVALLHALLGYALITGLAYNVVKKVAA
                     DLKTFNVEEEPPPPEEKPPPPPPEQPQTPPPIVTPPPIVRTNTPPPPMVTTNVAPPPV
                     ITYTAPPATPAPPAPAPPPPPPPPPPPRKVTPAKARANLASLFSDEDYPASAVRAGEQ
                     GATGFRLEVGANGRVTNCTVTSSSGSSALDSTTCRLLRSRARFSPATDSTGAPTADTS
                     TGRIVWRLPAD"
     gene            2151..2963
                     /locus_tag="VF547_02955"
     CDS             2151..2963
                     /locus_tag="VF547_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261814.1"
                     /translation="MAGLLAAAAVPAESQVAPASAGPTYADLADLALAAPVAAQVRVV
                     QAVAVKADRAPGLDPAMARFYVQAALVSLIRSPQSLPARLSYVVDLPRDAKGKPPKLR
                     KGSEFLLLAAPVAGRPGELRLIAPDSQVAFTPDRAATLRSIIREASSASPPPRIAGIG
                     RAFHVPGAIRGESETQFFLQTADGTPVSLTVLRRPGETASWAVALSEIVDEAAAAPAP
                     GTLLWYRLACGLPRTLPRQSLAEADAEGAAAIQADYRLILERLGPCVRSRAK"
     gene            complement(3056..3457)
                     /locus_tag="VF547_02960"
     CDS             complement(3056..3457)
                     /locus_tag="VF547_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX8261815.1"
                     /translation="MNEQVKLDGIVPAGLAPRSRKIVLDDYCISADIGFHDFEVGAPQ
                     RLLVTVEVWVDEASFPADDRPDGAWDYDFLRTEIGALIEGRRFNLQETVAREIYDLVA
                     ARRGVTALRVATRKPDIYPDCAGVGVELTSY"
     gene            complement(3450..4214)
                     /locus_tag="VF547_02965"
     CDS             complement(3450..4214)
                     /locus_tag="VF547_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8261816.1"
                     /translation="MASEFEGPRTAIVTGGARRIGAALCRALAEDGWNLLIHCRRSVD
                     EAEALARELGGASLVRADLGLPDAADRVMAALDGLPPARLLVNNASRFEFDTADDFTV
                     ERWDGILATNLRAPAMLSQAFAARVAGGGLIVNLLDAKLAAPNPDFFSYTVSKAGLAA
                     LTELCARAWAGRGLRVCGIAPAVTLLSGEQSQENFDSVHALNALGRGVRVEDIVAALR
                     FAIATPTLTGQTLTLDAGQRFLGLPRDVQFLEPGDE"
     gene            4289..4855
                     /locus_tag="VF547_02970"
     CDS             4289..4855
                     /locus_tag="VF547_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX8261817.1"
                     /translation="MPMPPPTDDLAGLSLAEIARLAEERRLPPVEQWNPTHCGDSEMR
                     IARDGTWFHQGSPIGRPAMVRLFSTILRREPDGRFVLVTPVEKLDIEVEDAPFVAVEL
                     RAEGAGEAASLAFRLNTGDVVVAGPEHPLRFEAGREGPHPYLGVRGGLEALIARPVYY
                     ELAELALAGGEPPGLWSGGAFFPMAPAR"
     gene            4852..5445
                     /locus_tag="VF547_02975"
     CDS             4852..5445
                     /locus_tag="VF547_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX8261818.1"
                     /translation="MSLADRLRHALERGRRRRPILLEGDVLEGAEGDLMPAAVLVAVV
                     DRPAPTVILTRRPETMRRHPGQISFPGGRIDPGDDGPVAAALREAEEEIGLPRDAVEV
                     VAMADPYRTITGFQVTPVVGVVPPGLPLEPQPGEVAAMFEAPLDYLLDPRHQIVRTVE
                     WGGRERRYYEIEWEGRRIWGATAAMIVNLSRRLEPAR"
     gene            5442..6650
                     /locus_tag="VF547_02980"
     CDS             5442..6650
                     /locus_tag="VF547_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX8261819.1"
                     /translation="MKLPKADWQRREGMKSLLAALDARGGATRYVGGCVRDTLLGLPV
                     SDVDLATRLLPEEAMARLKAAKIKVVPTGIAHGTVTAVVAGKPVEVTTLRRDVATDGR
                     RATIAYTDDWREDAARRDFTINALSADPVGLDVHDYFGGGEDLATRRVRFIGDPLTRI
                     AEDHLRILRFFRFFARFGAGEADGEALEACAARANDLMALSRERIADELTKLLALPEP
                     VEAVRLMIERGILRPVLPEIGSEGAERLARLVRREADAGVPPHPLRRLAALLPPDPAV
                     AAAVAARLRLSNRAAKRLAAAAGRAPGDGPSPELLAYRIGAEEAADRILLGEGDARAT
                     ARLAGWQRPRLRVGGGDLIAMGLVAGPAVARTLQAIEREWAEAGFPPEAAAQRAIARR
                     HVDQALRERQ"
     gene            complement(6626..7495)
                     /locus_tag="VF547_02985"
     CDS             complement(6626..7495)
                     /locus_tag="VF547_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8261820.1"
                     /translation="MLRNAQFALKRSKASGEIQIYEPNQARAARRRFSIETELRRSIE
                     AGGLSLAFQPLIELRTGAVAGFEALARWDHPHRGPISPAEFIPVAEESGLIVPLGQWA
                     LETALATLAHWDSAEGGALPFSVSVNLSALQLARDDLVGFVAAALARHGLAGDRLTLE
                     LTESAIVQDPRRATTVLGGLKALDVSVAMDDFGTGYTSLAYLQRLPIDVLKIDRSFVS
                     GMLRDTDSVAIVRAILSLASALGMATTAEGIDAPELAQALAEMGCSHGQGFHFARPLE
                     AGAALAYWRSRSA"
     gene            complement(7468..8388)
                     /locus_tag="VF547_02990"
     CDS             complement(7468..8388)
                     /locus_tag="VF547_02990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8261821.1"
                     /translation="MQAAKARGRAKSAAGRCPPQGGVPPELAPQFQQVLETVRQRYVD
                     ALPIAAAILTIADDSFIECANDPFRRLAEWDERRGERRIRAVPLLRSGPIGTRLAAFL
                     ESGDAAVQFDTTDGRSIAGRHFTVRFARLTLLPGQPARCMVSLLDKTAEVETERSLRA
                     EMLRDSLTGLPNRSAFNEEVAAVLEDPGFQPGSHAVLVIDMRRFSRVNECVGSLAGDE
                     LLITFARRLFSVLRGGDLLARTGGDEFGILMRLDRGLEEALQAAVRIEAALAMPFRLS
                     GSGSTVRSAARCSAAARRWPRRCCATPSSR"
     gene            8639..8887
                     /locus_tag="VF547_02995"
     CDS             8639..8887
                     /locus_tag="VF547_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261822.1"
                     /translation="MKKSFRRFAMVMLASIGGLTAPVPAVAQEDDPCLECHRNCYQAY
                     VVNSNQPATYQLCTNWCYQQYCYNYAPTGPELLAVRFD"
     gene            complement(8939..11170)
                     /gene="parC"
                     /locus_tag="VF547_03000"
     CDS             complement(8939..11170)
                     /gene="parC"
                     /locus_tag="VF547_03000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX8261823.1"
                     /translation="MSDASNLIDPIQNHTFEDALSKRYLVYALSTITARSLPDVRDGL
                     KPVHRRLLWAMRLLRLDPAAGYKKCARVVGDTIGKYHPHGDQSVYDAMVRLAQDFSLR
                     YPLVDGQGNFGNIDGDNAAAYRYTEARLTPAAIELMAGLEEGTVDFRPTYNGEEEEPE
                     VFPGLFPNLLANGASGIAVGMATSIPPHNLAEIIDAAVHLIDNPRAEPSALMDFVAGP
                     DFPTGGVIVDSAATIAAAYETGRGTFRVRGRWAVEDQGRGTWVAVISEIPYQVPKSKL
                     IEQIAALIADKKLPILADVRDESDAEIRIVVEPRSRTVEAGLLMDSLFRLTDLEVRVP
                     LNMNVLDADRTPRVMGLREVLSAWLAHQFEVLVRRAQHRVAKIEDRLELLEGYLVAFL
                     NLDRVIEIIRTEDEPKPVMVAEFALTDRQAEAILNMRLRQLRRLEEMQLRGERDDLIA
                     EREDLLKLIGSRDRQRTRLKKDLAGLKARYGPDRRTLIEEAAPAREIPLEAMIEREPI
                     TVIMSQRGWIRAMKGHVDPAEADALKFKEGDGARFFFHAQTTDKLLLAAADGRFYTLA
                     ADRLPGGRGFGEPVKLMADVEGEGNIVALFKAAVADKILLATSDGRGFVTSTAGAIAE
                     TRKGKQIVNLRPGARLRTVRPIGEGDDYVGVIGENRKMVVYPLSELPEMGRGQGVQLQ
                     RYRDGGLADATTFRFSDGLSWPMGGESGRVRTEADMNPWRAARGAAGRMPPMGFPKER
                     PFG"
     gene            11287..12192
                     /locus_tag="VF547_03005"
     CDS             11287..12192
                     /locus_tag="VF547_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX8261824.1"
                     /translation="MRKAICLSLLLATAACGQAQNDEAAPSTVGNMTTFDVEEAPPTA
                     TDASTSRSPRPPGVAPTAAPGVAFNYRYAFRLPAENIGRVQEEHAQACEKLGVEHCRI
                     TAMTYTDDGDRHIEAQLAFKLDPALARTFGRDGIAAVTKAQGELLQAAITGTDVGSEI
                     AAADRSQAQQADEIRRLEQQLARPGLSSSERVELQQQLQALRGSVRAGQAEQTGRRQR
                     LASTPVVFDYRAGKTGTRLERAIADAGDNFAGAAITVLVVLVTLLPWLAALLLLWLLW
                     RWLRRRIPLLQRSTAPAAEPSPPEA"
     gene            complement(12173..>12846)
                     /locus_tag="VF547_03010"
     CDS             complement(12173..>12846)
                     /locus_tag="VF547_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8261825.1"
                     /translation="SDLIDEDESLLVISTAQTFASNWLAGHLGAFQIARPELAVRLLT
                     SNGLVDFARDDVDVAVRMGDGQWPGLRKHFLFHHHFTPMCTPEFRDRPRLYRPEDVLR
                     APRLTPDDEWWKLWLNAAGVAEPAERTPGIRLDSQATEGQAAMAGHGLGMMTPMFWRA
                     ELAAGRLVRPFDLIACERRAHWLVYPEHKRGRAKIRAFRDWLLTEIAAEAPDGPAAAF
                     TPPAG"
BASE COUNT         1894 a         4424 c         4598 g         1930 t
ORIGIN      
        1 ctccggcttg gtctgggtgg gctcgaagtc gaccttggga agcttcatcg ggatcgtctg
       61 gatgacgact ggcacggtga tcaggaagat gatgaggaga accagcatga cgtcgacgag
      121 cggcgtcgtg ttgatgtccg acatcgggga ttcttcgccg gtttcactct cttggccgac
      181 attcatggcc atggtcggta ttcctatcta gctcgagtgc cgcggaaagc tgcgccggcg
      241 aaaccgaggg gcctcgccgg cgctattggt cagactttct tcgggccgcc atggggagcg
      301 ccgactgcgc ttccggtggc ggtggtcccg cccggcgagg ccgcgcccgg acccttggcc
      361 gctccggctc cggctgccgc tccgcccgca gcgccgccgg cgcggctggc gatgaccgga
      421 cggaccgctc cgcccgacat catgtagccg tgaagctcgt tggagaagcg ggcgagctcc
      481 tcgacgatcg ccttgttgcg gcggatcagc cagttgtagg cgagcaccgc cggaaccgcg
      541 acgacgaggc cgagcgcggt catgatcaga gcctctccga ccgggccggc gacggtgtcg
      601 atcgaggcct ggccggccgc gccgatcttc accagggcgc ggtagattcc gacgacggtt
      661 ccgaacagtc cgacgaacgg agcggtcgag ccgacggtgg cgaggaaggc gaggccgctc
      721 gacagccgcg aggagatcgc gccctggctg cgcgccagcg agttcagcat ccagtcgtgc
      781 tggtcgatcg gatcggtcag cttgtcatgc tgctcgaggg cgaggatgcc gtcgtcgacg
      841 atctggcgat aggcgctgtt cttctcgagc ttggaagcgc cttcgcgcag gttggccgac
      901 tgccagaagc tggtccgggc gcggcgaccc tggttgatga tcttctgctg ctcgaacagc
      961 ttgacgaaca gcacgtacca ggaaccggcc gacatgatca cgaggatgat gaaggtggcc
     1021 caggcaatcg cgccgccctg ctcgagtgcg gccatgaggc cataagggtt gtcgccgccg
     1081 tgggctgcgc ctgctgctgg ggtggccatg gtttctactt tcctctcgat agataatgaa
     1141 aaatcgtgat ctgaagtcga tccatcctca gtcggccggc agacgccaga cgattcggcc
     1201 ggtcgaggtg tcggcggtcg gagccccggt gctgtcggtc gcgggcgaga aacgggccct
     1261 ggacctcaga agccggcagg tcgtcgagtc gagcgcggaa gagccgctgg acgacgtcac
     1321 cgtgcaattg gtgaccctgc cgttggcacc gacttcgagc ctgaacccgg tcgcaccctg
     1381 ctctccggct cggacggccg aagccggata atcctcgtcg gagaaaagcg acgccaggtt
     1441 cgccctcgcc ttggccggag tcaccttccg cgggggcgga ggcggcggcg gcggcggcgg
     1501 cggcgccgga gcgggaggcg ccggggtcgc gggcggtgcc gtataggtga tgaccggcgg
     1561 cggagccacg ttggtcgtca ccatcggcgg cggcggcgtg ttcgtccgaa cgatcggcgg
     1621 aggagtcacg atcggaggcg gagtctgggg ctgttccggc ggcgggggcg gcggcttctc
     1681 ctcggggggc ggcggctcct cctcgacgtt gaacgtcttg aggtcagccg ccaccttctt
     1741 gaccacatta taggccaggc cggtgatcag tgcgtagccg agaagagcgt ggagcagagc
     1801 gacgatgaag atcgccgaca tccggctctt gctcatcctg ttgtcagcat atgccattaa
     1861 tcagccacac tccctctctt cgtccctctg ccggggaccc gcgcgtcatt aacaacgctg
     1921 ctaaacccca acggctccat ccgaaatgcg atgtatcaac atatcatcga ctggagcggg
     1981 ttcctctatc gtgagactaa ggaggacgca atcctccagt gaaggcatcg cacttacccc
     2041 caaaacggcc gaaatccgcc gttcatcggg ctaagcatat gggaaacggg acataaactt
     2101 acggaggcgg aacggtggca caggcgctaa ctttttgtaa acggacgctg atggccggac
     2161 tgctggctgc ggcggcggtc ccggccgaat cgcaggtcgc tccggcttcg gcggggccca
     2221 cctacgccga tctcgccgat ctcgctctcg ccgcaccggt cgcggcccag gtccgcgtgg
     2281 tccaggccgt cgcggtcaag gccgaccggg cgccgggcct cgatcccgca atggcacgct
     2341 tttacgttca ggcggcgctc gtctcgctga tccgctcgcc tcagtccctc ccggcccggc
     2401 tcagctacgt cgtggacctg ccgcgcgatg ccaagggcaa gccgccgaag cttcgcaagg
     2461 gcagcgagtt ccttctcctc gccgcgccgg tggccggccg gccgggcgag ctccggctga
     2521 tcgctccgga ttcgcaggtc gccttcacgc cggaccgggc ggcgaccctt cgctcgatca
     2581 tccgcgaagc gagctcggcc tcgccgccgc cccggatcgc cgggatcggc cgggccttcc
     2641 atgttccggg cgcgatccgg ggcgagagcg agacccagtt cttcctccag accgcggacg
     2701 gaaccccggt atcgctgacg gtgcttcgcc gccctggcga aaccgcgtcc tgggcggtgg
     2761 cgctcagcga gatcgtcgac gaggccgccg cggctccggc gccgggcacc ctgctctggt
     2821 accgcctcgc ctgcgggctt ccgcgcaccc tgccgaggca gagccttgcc gaagcggatg
     2881 ccgagggcgc cgcggcgatc caggcggatt ataggctgat cctcgagcga ttggggcctt
     2941 gcgtgaggag tcgggcgaag tgacccgtca ccccggcgaa agccggggcc tcgtggggat
     3001 tggccgcggc gcctttaccg acccgaggtc ccggctttcg ccgggatgac gatggtcaat
     3061 aggaggtgag ctccaccccg accccggcgc agtcggggta gatgtcgggc tttcgggtgg
     3121 cgacccgaag cgcggtcact ccgcgccggg cggcgacgag atcgtaaatc tcccgggcca
     3181 ccgtctcctg gaggttgaac cggcggcctt cgatcagggc gccgatctcc gttcggagga
     3241 agtcgtagtc ccaagccccg tcgggccggt cgtcggccgg gaagctggcc tcgtcgaccc
     3301 agacctcgac cgtgaccagc agccgctgcg gcgctccgac ctcgaaatcg tggaagccga
     3361 tgtcggccga gatgcaataa tcgtcgagca cgatcttgcg gcttctgggc gcaagcccgg
     3421 ccggaacgat cccgtcgagc ttcacctgct cattcatcgc ccggctccag gaactgaacg
     3481 tcgcggggca gtccgaggaa gcgctgcccg gcgtcgaggg tgagcgtctg cccggtcagc
     3541 gtcggcgtgg caatggcgaa gcgcagggcg gcgacgatgt cctcgacccg cacgccccgg
     3601 ccgagcgcgt tgagggcgtg gacggagtcg aaattctcct ggctctgctc gccggacagc
     3661 agcgtcaccg ccggggcgat gccgcagacc cttaggccgc ggcccgccca ggcccgggcg
     3721 cacagctcgg tgagggcggc gaggccggcc ttcgagaccg tgtaggagaa gaaatcggga
     3781 ttgggcgcgg cgagcttggc gtcgagcagg ttgacgatca ggccgccccc ggcgactcgg
     3841 gcggcaaagg cctgcgacag catcgccggc gcccgcagat tggtggcgag gatcccgtcc
     3901 cagcgctcga ccgtgaaatc gtcggcggtg tcgaactcga agcgcgacgc attgttgacc
     3961 agcagccgcg ccggcggaag tccgtccagg gccgccatca cccggtccgc cgcgtcgggc
     4021 aggccgaggt cggccctgac cagcgacgcg ccgcccagct cgcgggccag ggcttcggcc
     4081 tcgtcgaccg agcggcggca gtggatcagc agattccagc cgtcctcggc cagcgcccgg
     4141 cacaaggccg cgccgatccg cctggcacct ccggtgacga tcgccgtccg cggcccttcg
     4201 aactcgctgg ccatggcgcc ggataggatg gacggcggaa ggcggcaagg cttgccgcgg
     4261 cggccaagcc ccgccagagt cgctcgccat gccgatgccg ccgcccaccg acgacctcgc
     4321 cggcctttcg ctcgccgaga tcgctcgcct cgcggaggag cgcaggctgc cgccggtcga
     4381 gcagtggaac ccgacgcatt gcggcgacag cgagatgcgc atcgcccgcg acggcacctg
     4441 gttccaccag ggcagcccga tcggacggcc ggcgatggtg cgcctgttct cgaccatcct
     4501 caggcgcgag ccggacggac gcttcgtgct ggtcaccccg gtcgagaagc tggacatcga
     4561 ggtggaggac gcgccgttcg tggcggtcga gctccgggcc gagggcgcgg gcgaggccgc
     4621 gtcgctcgcc ttccggctca acacggggga cgtcgtcgtg gcgggtcccg agcacccgct
     4681 gcgcttcgag gccggccggg aagggccgca cccctatctg ggcgtccgcg gcgggctcga
     4741 ggcgctgatc gcccggccgg tctattacga gctcgccgag ctcgcgctgg ccggcggcga
     4801 gccgcccggg ctgtggagcg gcggcgcctt cttcccgatg gcgccggcac ggtgagcctg
     4861 gccgaccggc tgcgccacgc cctcgagcgc gggcggcggc gccggccgat cctgctggaa
     4921 ggcgacgtgc tcgagggggc cgaaggcgac ctgatgccgg cggcagtgct ggtggccgtg
     4981 gtcgaccgcc cggcgccgac ggtgatcctc acccggcggc cggagacgat gcgccggcat
     5041 cccgggcaga tcagcttccc cggcggccgg atcgacccgg gcgacgacgg ccccgtcgcc
     5101 gccgcgcttc gagaggccga agaggagatc ggcctccccc gcgacgccgt cgaagtggtt
     5161 gcaatggccg acccgtaccg gacgatcacc ggcttccagg tgacccccgt ggtcggagtc
     5221 gtgccccccg gcctcccgct cgagccgcag ccgggcgagg tcgcggcgat gttcgaggcc
     5281 ccgctcgatt atctcctcga tccccgccac cagatcgtcc ggacggtcga atggggcggc
     5341 cgcgagcgcc gctattacga gatcgagtgg gaggggcgcc ggatctgggg cgcgaccgcg
     5401 gcgatgatcg tcaacctgtc gcggcggctg gagccggcgc gatgaagctc cccaaagcgg
     5461 actggcagcg gcgcgagggc atgaaatccc tgctcgcggc gctcgacgcc cggggcggcg
     5521 ccacccgtta tgtcggcggc tgcgtgcgcg acaccctcct cggcctgccg gtcagcgacg
     5581 tcgatctcgc cacgcgcctt ctcccagagg aagcgatggc ccggctgaag gcggcgaaga
     5641 tcaaggtggt gccgaccggc atcgcccacg gcaccgtcac cgccgtcgtc gccggcaagc
     5701 cggtcgaggt gacgacgctc aggcgggacg tcgccaccga cggccgccgc gccaccatcg
     5761 cctataccga cgactggcgc gaggacgcgg cccggcgcga tttcaccatc aacgccctct
     5821 ccgcggaccc ggtcggcctc gacgtccacg attatttcgg cggcggcgag gaccttgcga
     5881 cccgccgggt gcgcttcatc ggcgatccgc tcacccgcat cgccgaggac catctgcgga
     5941 tcctgcggtt cttccgcttc ttcgcccgct tcggagccgg agaggcggac ggcgaggcgc
     6001 tggaagcctg cgcggcccgg gccaatgacc tgatggcgct gtcgcgcgaa cggatcgcgg
     6061 acgagctgac caagctgctc gccctgcccg agccggtcga ggccgtccgg ctgatgatcg
     6121 agcgggggat attgcggccg gttcttccgg agatcggctc cgagggggcc gagcggctgg
     6181 cccggctggt ccggcgcgag gccgacgcgg gcgtgccgcc gcatccctta cgccggctcg
     6241 ccgccctgct gccgcccgat ccggcggtcg cggcggcggt ggcggcgcgg ctgcggctgt
     6301 cgaaccgggc cgccaagcgg ctggccgcgg cggctgggcg cgcgccgggc gacgggccgt
     6361 cgccggaact gctcgcctat cggatcgggg ccgaggaagc cgccgaccgg atcctgctcg
     6421 gcgagggcga cgcccgggcc acggcgaggc tggccggctg gcagcggccg cggcttcgcg
     6481 tcggcggcgg cgacctcatc gccatgggcc tcgtcgcggg cccggcggtg gcgcgcacgc
     6541 tgcaggcgat cgagcgcgaa tgggcggagg cgggctttcc gccggaagcc gcggcgcaac
     6601 gggcgatcgc ccgccgccat gtcgatcagg cgcttcgcga gcgccaatag gcgagggccg
     6661 ccccggcctc gagcgggcgc gcgaaatgga agccctggcc gtgcgagcag cccatctcgg
     6721 ccagggcctg cgccagctcc ggcgcgtcga tcccctcggc ggtcgtcgcc attccgagtg
     6781 cgctggcgag cgacaatatg gcccgcacga tggcgaccga atccgtgtcg cggagcatcc
     6841 ccgagacgaa gctgcggtcg atcttgagca cgtcgatcgg cagccgctgc agataggcca
     6901 gcgaggtgta gccggtgccg aaatcgtcca tcgccacgct gacgtcgagc gccttgaggc
     6961 cgcccagcac cgtcgtcgcc cggcgcgggt cctggacgat cgcgctctcg gtgagctcga
     7021 gcgtcagccg gtcgccggcg aggccgtggc gggccagggc cgccgcgacg aagccgacga
     7081 ggtcgtctcg cgcgagctga agcgcggaaa ggttgacgct gaccgagaag ggcagcgccc
     7141 cgccctcggc cgaatcccag tgcgcgaggg tcgccagggc ggtctccagc gcccattggc
     7201 cgagcggaac gattagcccc gattcctcgg cgaccgggat gaactcggcc ggcgagatcg
     7261 gccccctgtg cggatggtcc cagcgggcca gcgcctcgaa gccggcgacg gccccggtgc
     7321 gaagctcgat cagcggctgg aaggcgagcg aaagtccgcc cgcctcgatc gagcgcctga
     7381 gctcggtctc gatgctgaat cgccgccgag cggcgcgcgc ctggttgggc tcgtagatct
     7441 ggatctcccc gctcgccttg gagcgcttca gcgcgaactg ggcgttgcgc agcacctcct
     7501 cggccagcgc ctcgctgccg ctgagcagcg cgcagccgag cgcacagtcg acccggatcc
     7561 ggagagccgg aagggcatcg ccagcgccgc ttcgatccgg accgcggcct ggagcgcttc
     7621 ctcgagcccg cggtcgagcc gcatcaatat tccgaactcg tcgccgccgg tgcgggcgag
     7681 caggtcgccg cctcgcagca cggagaagag ccggcgcgcg aaggtgatca gcaattcgtc
     7741 gccggcgagc gagccgacgc attcgttgac ccggctgaag cggcgcatgt cgatgaccag
     7801 gacggcatgg ctgccgggct ggaagccggg atcctccagc accgccgcca cctcctcgtt
     7861 gaacgcgctg cggttgggca ggccggtgag gctgtcgcga agcatctcgg cgcgcaggct
     7921 gcgctcggtc tcgacttcgg ccgtcttgtc gagcagcgag accatgcagc gggcgggctg
     7981 cccgggcagc agggtgagcc gagcgaagcg gacggtgaaa tggcggccgg cgatgctccg
     8041 gccgtcggtc gtgtcgaact ggacggccgc gtcgccgctc tcgaggaagg cggcgagacg
     8101 ggtcccgatc ggcccggagc ggagcagcgg caccgcgcgg atccggcgct cgccccgccg
     8161 ctcgtcccat tcggcgaggc ggcggaaggg atcgttggcg cattcgatga agctgtcgtc
     8221 ggcgatggtg agaatggcgg cggcgatcgg aagcgcgtcg acataacgct ggcggaccgt
     8281 ctccagcacc tgctggaatt gcggcgccag ctcgggcggc acgccgccct gcggcgggca
     8341 ccgtcccgcc gccgactttg ctcgcccccg cgctttcgct gcctgcatgg gaatcgcggt
     8401 agagaaatag ggttaacgca tcgttgagaa cgcctgcgta agggtcggcc gaagcggtcg
     8461 ttcctggccc ggtcggcgcc cccctgcgcg aagtccccgg acgcgtcggg ccgctgccat
     8521 tgtggctaag tgtttgcctc catttcggaa cttgttgccg ttgaccgtcc ctgacgcggc
     8581 ctccagcctc ccctcgccga actttccgtg cggcgcgcct gttgggagga gagacctgat
     8641 gaagaaatct tttcgccgtt tcgcgatggt gatgctggcc tcgatcggcg gcctgacggc
     8701 accggttccg gccgtggcgc aggaagacga cccgtgcctg gagtgccacc gcaattgcta
     8761 tcaggcctat gtcgtcaact cgaaccagcc ggccacgtat caattgtgca caaactggtg
     8821 ctatcagcag tattgctaca actatgcgcc gaccggcccg gagctgctcg ccgtccggtt
     8881 cgactgagcc cgagcgcgaa ggagacgcgg gcccgcccca ggggcgggtc cgttcccttc
     8941 agccgaaagg cctttctttc gggaagccca tcggcggcat ccgcccggcg gcgcctcggg
     9001 ccgcgcgcca ggggttcatg tcggcctcgg tccgcacccg gccgctctcg ccgcccatcg
     9061 gccagctcag cccatcggag aagcggaagg tggtggcgtc ggcgaggcct ccgtcgcggt
     9121 agcgctgaag ctggacgccc tggccccggc ccatctccgg gagctcgctg agcgggtaga
     9181 cgaccatctt ccggttctcg ccgatcaccc cgacataatc atcaccctcg ccgatcggcc
     9241 gcaccgtcct gagccgcgcg cccggccgaa ggttgacgat ctgcttgccc ttgcgggtct
     9301 cggcgatcgc gccggcggtc gaggtgacga agccgcgccc gtccgacgtc gccagcagga
     9361 tcttgtcggc gacggccgcc ttgaacaggg cgacgatatt cccctcgccc tcgacgtccg
     9421 ccatcagctt gacaggctcc ccgaagcccc ggccgccggg cagccggtcc gccgccagcg
     9481 tgtagaagcg gccgtcggcg gcggcgagga gcagcttgtc ggtggtctgg gcgtggaaga
     9541 agaaacgcgc gccgtcgccc tccttgaact tgagcgcgtc ggcctcggcc gggtcgacat
     9601 ggcccttcat cgcccggatc cagccgcgct gcgacatgat gaccgtgatc ggctcgcgct
     9661 cgatcatggc ctcgagcggg atctcgcgcg ccggcgccgc ctcctcgatc agggtcctcc
     9721 ggtccggacc gtagcgggcc ttgaggccgg cgagatcctt cttgagccgg gtgcgctggc
     9781 ggtcgcgcga tccgatcagc ttcaacaggt cctcgcgctc ggcgatgagg tcgtcgcgct
     9841 cgccgcgaag ctgcatctcc tcgagccggc gcagctggcg cagccgcatg ttgaggatcg
     9901 cctcggcctg gcggtcggtg agcgcgaact cggcgaccat caccggcttg ggctcgtcct
     9961 cggtgcggat gatctcgatc acccggtcga ggttgaggaa ggcgaccaga tagccttcga
    10021 gcagctcgag ccggtcctcg atcttcgcga cccggtgctg ggccctgcgg acgagcacct
    10081 cgaactggtg cgcgagccat gcggacagca cttccctgag ccccatcacc cgcggcgtgc
    10141 ggtccgcgtc gagcacgttc atgttgagcg gcacccgcac ctcgaggtcg gtcagtcgga
    10201 acaggctgtc catcagcagg cccgcctcga cggtgcggct ccggggctcg acgacgatcc
    10261 ggatctcggc gtccgattcg tcgcggacgt cggcgaggat cggcagcttc ttgtcggcga
    10321 tcagggccgc gatctgctcg atcagcttcg acttgggcac ctggtaggga atctccgaga
    10381 tcacggccac ccaggtgccc cgcccctgat cctcgacggc ccagcggccg cggacccgga
    10441 aggttccgcg gccggtctcg taggccgccg cgatcgtggc ggcgctgtcg acgatgaccc
    10501 cgccggtcgg gaaatccggg ccggcgacga agtccatcag cgccgagggc tcggcgcggg
    10561 gattgtcgat caggtggacg gccgcgtcga tgatctcggc gaggttgtgc ggggggatcg
    10621 aggtggccat gccgaccgcg atgccgctgg cgccgttggc gagcaggttg gggaacaggc
    10681 cggggaagac ctcgggctcc tcctcctcgc cattgtaggt cggccggaag tcgaccgtgc
    10741 cttcctcgag cccggccatc agctcgatcg cggccggggt cagcctcgcc tccgtatagc
    10801 ggtaggcggc ggcgttatcg ccgtcgatat tgccgaaatt gccctggccg tcgacgagcg
    10861 ggtagcgcag cgagaagtcc tgggcgaggc ggaccatcgc gtcgtagacc gactggtcgc
    10921 cgtgcggatg atatttgccg atcgtgtcgc cgaccacgcg cgcgcatttc ttgtagccgg
    10981 ccgccggatc gaggcggagc agccgcatcg cccatagcag acggcgatgg accggcttca
    11041 gcccgtcccg gacgtcgggc agggagcggg cggtgatcgt cgacagggcg tagaccaggt
    11101 agcgcttcga cagggcgtct tcgaaagtgt ggttctggat cggatcgatg aggttcgagg
    11161 cgtcggacat ggccgaccga tagcagcgcg attcgaggga ggcgactggt ctattgcgtt
    11221 tgtctactga taagataatg ttacacagcc ggcggaagac cggtcctgaa cgagaggaga
    11281 agaaggatgc gcaaggcgat ctgtttgagt ctcctgctgg ccaccgccgc ctgcggccag
    11341 gcccagaacg acgaggccgc gccgtcgacg gtcggcaaca tgacgacctt cgacgtggag
    11401 gaagcgccgc cgacggcgac ggacgccagc acctcgaggt cgccccggcc gccgggagtc
    11461 gcgccgaccg ccgcgcccgg agtcgccttc aactatcgct acgccttccg ccttcccgcc
    11521 gagaatatcg gccgcgtcca ggaggagcat gcccaggcct gcgagaagct gggagtggag
    11581 cattgccgga tcacggcgat gacctacacc gacgacggcg accgccacat cgaggcgcag
    11641 ctcgcgttca agctcgaccc cgcgctggcc cgcacgttcg gacgggacgg aatcgcggcc
    11701 gtcaccaagg cccaaggcga gcttctgcag gcggcgatca ccggcaccga cgtcggcagc
    11761 gagatcgcgg cggccgaccg cagccaggcg cagcaggcgg acgagatccg ccggctcgag
    11821 cagcagcttg cccggcccgg cctcagctcg agcgaacggg ttgagctcca gcagcagctc
    11881 caggcgctga ggggctcggt ccgcgccggc caggcggagc agaccggccg ccgccagcgt
    11941 ctggccagca ccccggtggt gttcgactat agggccggca agacgggaac ccggctggag
    12001 cgggccatcg ccgacgccgg cgacaatttc gccggcgccg ccatcaccgt gctggtggtg
    12061 ctggtcacgc tgctgccctg gctggcggcg ctgctgctgc tctggctgct gtggcggtgg
    12121 ctccgtcgcc ggatcccgct gctgcagcgc tcgaccgcgc ccgccgccga gcctagcccg
    12181 ccggaggcgt gaacgcggcg gccgggccgt ccggggcttc cgcggcgatc tccgtcagga
    12241 gccagtcgcg gaaggcccgg atcttggccc ggccccgctt atgctcgggg tagacgagcc
    12301 agtgcgcccg ccgttcgcac gcgatcaggt cgaagggcct gaccagccgg ccggcggcga
    12361 gctcggcgcg ccagaacatc ggggtcatca tgccgagccc atggcccgcc atcgcggcct
    12421 ggccctcggt cgcctgggaa tcgagccgga tgccgggggt tcgctcggcc ggctcggcca
    12481 cgccggcggc gttgagccat aatttccacc actcgtcgtc gggcgtgaga cgcggtgccc
    12541 gaagcacgtc ctcgggccgg tagagtcgag ggcgatcgcg gaattccggc gtgcacatcg
    12601 gcgtgaaatg gtggtggaag aggaaatgct tccgaaggcc cggccactgc ccgtccccca
    12661 tccgaaccgc gacgtcgacg tcgtcccggg cgaagtcgac caggccgttg ctggtcagca
    12721 ggcgcaccgc cagctccggc cgagcgatct ggaaggcgcc gagatggccg gccagccagt
    12781 tcgacgcgaa ggtttgcgcg gtgctgatca ccagcaggct ctcgtcctcg tcgatcaggt
    12841 ccgaga
//
LOCUS       DASVQZ010000110        10714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_121936, whole genome shotgun sequence.
ACCESSION   DASVQZ010000110 DASVQZ010000000
VERSION     DASVQZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121936"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2600)
                     /locus_tag="VF547_03015"
     CDS             complement(<1..2600)
                     /locus_tag="VF547_03015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX8261826.1"
                     /translation="MTGAFPYPALHATHGGIWLASTDGEVRGLGRGEAIARAAETPLV
                     MLNAPLVGQRLGYPDLSGLDLLELFAFVHPARFAVPTPKGLAEALRIEAPANDSEAAS
                     FLLRAAAALLATLERDWPEREGAWASAQALWRLRWSWAPAVAQRLKRPERDERWLFSK
                     LPEWEESGARPAPRTVLVPQDRAVEPLARRVGSDAEVREGQRDYAAAAAAAFDPRKVE
                     GQPNLLLAEAGTGIGKTLGYLAPASLWAESADGAVWVSTFTKALQRQLDREGLRLFPD
                     PEARRRKVVVRKGRENYLCLLNLEDALQGGFAGRAAILAQLVARWAAWSKDGDMVGGD
                     MPGWLPTLFRRAGSAALTDRRGECVYAGCPHYRKCFIERSARASQDADIVIANHALVM
                     VNAARGRDGGNPPTRIVFDEGHHLFEAADSTFAAALTGQEAIELRRWIVGPEGKSRGR
                     RRGLAARLMDVASYDEAGALALEAAVQAAGLLPGDGWLQRLAEGMAFGPVEKLLAAVR
                     GTVYARATAQDAGYGIETEMAELDGDVVAAAAPAVEALEQLMRPLMALARRLEAVLED
                     GPDWLDSQARARIEGAIGGLAWRTQTIAAWVALAARLGGPADADFVDWLAVDRIDGRE
                     FDIGLHRRWLDPTRPLADSVLKPAHSVIVTSATLRGAEGWGGAEARTGAVHVPGAVRH
                     FSAPSPFDYAANSEVLIVTDVKRGDVGQLAGAYSRLIEAAEGGTLGLFTAIQRLKAVH
                     ARIADRLARAGLPLYAQHVDPIDTGTLVDIFRDDPHASLLGTDALRDGVDVPGHSLRL
                     VVMEGVPWPRPTVLHAARRAAGGGTAYDDRVVRARLAQAFGRLIRRSEDRGVFVLLSA
                     ATPSRLLAAFP"
     gene            complement(2671..3672)
                     /locus_tag="VF547_03020"
     CDS             complement(2671..3672)
                     /locus_tag="VF547_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8261827.1"
                     /translation="MRPVPEYHDVDRERFERDIVPAGRPAVLRGLCAGWPATRCDGDE
                     ALAAYLRRAAADEPFEAWFGPPEIAGRFGYAADFQGFNHDRRMATVDQLLDLLLRQRG
                     HPNPFSIYAGAIPIARHLPGLKADVPMPLLDPGREKLVSLWLGNRTRTAAHWDLPQNL
                     ACVVAGRRRFTLFPTDQVRNLYVGPLDFTLAGQPTSLVDLEAPDFERHPRFREALEAA
                     ETAELGPGDALYLPSLWWHGVESLDELGAMVNFWWRDGEPPLLTPLNALYHAAITVKD
                     LPPRERAAWRALFDFYVFQDHGEPAAHLPESARGLLGRRTPELVARVKAMLARALGR"
     gene            3720..5303
                     /locus_tag="VF547_03025"
     CDS             3720..5303
                     /locus_tag="VF547_03025"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX8261828.1"
                     /translation="MSEKEAIRAAALASKAWPYEEARKLLKRYPDGPPAGEIVFETGY
                     GPSGLPHLGTFQEVARTLMVRHALAEMVDWPSRLIAFSDDMDGMRKVPPGVPGQDMMH
                     AHLDRPLSLVPDPYGCSHDSYAAHNNLLLRQFLDRFGFDYEFLASSDCYRSGRFDETL
                     RQVLRRYDSIMAVMLPTLGEERRRTYSPVLPISPESGKVLQVPIEVVDAEAGTVRYTD
                     PATGQAVEQSILSGGAKLQWKVDWAMRWAALGVDYEMSGKDLIDSVVQSSKIARILGA
                     RPPEGFNYELFLDENGEKISKTRGNGVTLDEWLTYAPEESLVFYLYREPRKAKQLSFG
                     IIPKAVDEYHQFLAAYADQEWDKRLGNPVHHVHAGKVPEARMPLSFALLLNLVGVAST
                     DDKDLLWGFVKRYAPEASPETHPELDALIGHALAYFRDFVAGSLRRRAPDEREAAALR
                     ALDSKLAAMPEDASAEDIQTEVYEVGKAAGFDNLRDWFRAIYETLLGTSQGPRMGSFV
                     ALYGIANSRRLIAEALASA"
     gene            complement(5325..5762)
                     /locus_tag="VF547_03030"
     CDS             complement(5325..5762)
                     /locus_tag="VF547_03030"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEX8261829.1"
                     /translation="MRARKPLWTCPACGRAFANANQSHACGRHALDGHFAGKPPLVRD
                     IYDAFLAMLEEMGPVTVLPEKTRIAFQARISFAQLTVRRRWVLGHFVLARRLEDPLFS
                     KVETISPRNHVHHFRLERVEQVDGLRGFAREAYAVGRQEHLRS"
     gene            5820..6170
                     /locus_tag="VF547_03035"
     CDS             5820..6170
                     /locus_tag="VF547_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX8261830.1"
                     /translation="MNPAGHAIEITPFGGRVRVRVAGEMLADTAHALTLEEGSLPPVF
                     YIPRADVHMDRLSPSATTSHCPFKGDASYFSAASGPADVAWSYEAPLAQASAIAGHLA
                     FYPDQAEIETVPFG"
     gene            complement(6268..8088)
                     /locus_tag="VF547_03040"
     CDS             complement(6268..8088)
                     /locus_tag="VF547_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8261831.1"
                     /translation="MRGSLSIGLTAALLAGCASSQASPAPEAAAAPAPRQTASQALAA
                     LFRDSDEAGLQRNPIQALARGDLRYADRFGDFFSDSYYAAERAAARAELARLAQIDRD
                     ALGADERVAYDVFKWQRGMDLKGLEGEIFAASVVRPIDHFNGFHTFFVETHSGEGIAP
                     YKTLKDYENGLSRFDDFVSLADGAIARMEQGLAAGVVQPKLVMANVVEQLDAMLAEGV
                     EGSSLYRPVANFPAEVPEADRPRLKAAYAEAISTKIRPAVTRLRDFIRDRYLPRARDS
                     VGLQDMKGGPALYRHLVALSTTTDMTPDEIHRIGLAEVARLHAEMEAVKARVGFTGTL
                     QQFFEHIRTDPRFKPASREALQQGYVAIGKRLDATLPTMFSTIPKTRLEIRPVPALTE
                     KGAARGSYQSGTTDGSRPGVFYFNAYDLPSRTTPGMETLYLHEGAPGHHFQISLAQEN
                     EALPSFMRFGGNTAYVEGWGLYAETLGRELGVYTDPYQYFGYLDSQLFRAIRLVVDTG
                     IHSKGWTRDQTIQYILDNSSRGRSNATAETERYIAIPGQALAYKIGQLKISELRARAE
                     KALGPRFDIREFHAQVLMTGALPLAVLEKKIDDWIAAKKG"
     gene            complement(8164..9984)
                     /locus_tag="VF547_03045"
     CDS             complement(8164..9984)
                     /locus_tag="VF547_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8261832.1"
                     /translation="MKPSFFLLAAVSLAAVSAPLAAQGGASAPAAPAAAESEGAKLAR
                     LFHESDEANLKRNPVGAIFRGDLRYADRLGDFITDAYFNAERAASEGDLAALRRIDRS
                     KLSETDRIAYDVFEWQTRDALKNLEPRMLALGIVRPINHFSGFHTFYPAFASGEGAAP
                     FKTVADYENNLKRHKDYVLFHDRAIGRFREGMESGVFETKLTTQNVIDQLTLLLDQKV
                     EDSPFYGPVKRFPADFSEADKTRLAAAYRASIESEIYPVHRRVRDFLKTEYLPKAREG
                     VGLVHMKGGPEVYRRLIESTTTLPLTADEVHSTGLSEVARIKSEMEAVKTRAGFKGTL
                     PQFFEYLRTDPKFKPASKEALREGYEAIARRVDARIREQFSTIPKSPLEIRPVEPFRE
                     KTEAGGSYQQGTPDGSRPGIFYYNSYDLPSRRLWGMETLYLHEGSPGHHFQISLAQEN
                     EALPAFMRFGGNTAYVEGWALYAETLWDELGMETDPYQRFGALNDEMLRAMRLVVDSG
                     IHAKGWSRDQAIDYMLANSGMSRTEVTSEVERYIAIPSQALAYKVGALTIQRLKAKAQ
                     KGLGARFDPREFHAQVLMTGSLPMGVLEKKIDDWIASKKA"
     gene            complement(10057..>10714)
                     /locus_tag="VF547_03050"
     CDS             complement(10057..>10714)
                     /locus_tag="VF547_03050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8261833.1"
                     /translation="TYVRERVLKTPNAFKIPAQGLDIFVVRQFLTPAECGEVIELIDK
                     DRIPSGLLAPSPDPEFRTSESCNLRLEDRANQMVEAKINRLTGIDPIHGETIQGQRYA
                     VGQQFKPHHDFFYTNQPYWPEMERQGGQRTWTAMIFLNEPEAGGQTFFPKANVRVTPR
                     TGNLLLWNNLDQYGEPNTYSLHTGCPVEAGTKYVITKWYRERPWGYYGEQNVKPTAYY
                     "
BASE COUNT         1608 a         3614 c         3846 g         1646 t
ORIGIN      
        1 gggaaggcgg caagcagacg gctcggagtc gccgcggaca gcagcacgaa gacgccccga
       61 tcctcgctcc ggcggatgag gcggccgaac gcctgggcga gccgggcgcg gaccacccgg
      121 tcgtcatagg cggtgccgcc gccggcggcc cgccgggcgg cgtggagcac ggtcggccga
      181 ggccagggca cgccctccat caccaccagg cgcagcgaat gccccggcac gtccactccg
      241 tcgcgaagcg cgtcggtgcc gagcagcgag gcgtgcggat cgtcgcggaa gatgtcgacc
      301 agggtgccgg tgtcgatcgg gtcgacatgc tgggcgtaga gcggaaggcc ggcgcgggcc
      361 agccggtcgg cgatccgggc atggaccgcc ttcagccgct ggatcgcggt gaacaggccg
      421 agcgtgccgc cctcggccgc ctcgatcagc cgggaatagg cgccggcgag ctggccgacg
      481 tcgccgcgct tgacgtcggt gacgatcagc acctcgctgt tcgccgcata gtcgaacggg
      541 ctgggggcgc tgaagtggcg caccgccccg ggcacgtgga ccgctccggt gcgcgcctcg
      601 gcgccgcccc agccctccgc tccgcgcagc gtcgccgagg tgacgatgac gctgtgggcg
      661 ggcttcagca ccgagtcggc gagcggccgg gtcggatcga gccagcgccg gtgaaggccg
      721 atgtcgaact cgcggccgtc gatccggtcg acggccagcc agtccacgaa atcggcgtcg
      781 gccgggccac cgagccgcgc cgcgagcgcc acccaggcgg cgatcgtctg agttctccac
      841 gccaggccgc cgatcgcgcc ctcgatccgg gcccgcgcct gcgaatcgag ccagtccggg
      901 ccgtcctcga gcaccgcctc gagccgcctc gcgagcgcca tcagcggccg catcagctgc
      961 tccagcgctt ccaccgcagg ggccgcggcg gcgacgacgt cgccgtcgag ctcggccatc
     1021 tcggtctcga tgccatagcc cgcgtcctgc gcggtggcgc gggcgtagac cgtgccccgg
     1081 accgccgcca gcagcttctc gacgggcccg aaggccatgc cctcggcgag ccgctggagc
     1141 cagccgtcgc cgggcagcag gcccgccgcc tggaccgcgg cctcgagcgc gagcgccccg
     1201 gcctcgtcgt aggaggcgac gtccatcagc ctcgccgcca gcccgcgccg ccgcccgcgc
     1261 gatttgccct cgggcccgac gatccatcgc ctcagctcga tcgcctcctg gccggtcagc
     1321 gccgccgcga aggtggaatc ggcggcctcg aacagatgat ggccctcgtc gaagacgatc
     1381 cgggtcggcg ggttgccgcc gtcgcggccg cgcgccgcgt tgaccatcac cagcgcatgg
     1441 ttggcgatga cgatgtcggc gtcctggctc gcccgggccg agcgttcgat gaagcatttg
     1501 cggtaatgcg ggcagcccgc atagacgcat tcgccgcgcc ggtcggtgag cgcggccgag
     1561 ccggcgcggc ggaacagggt cggaagccag ccgggcatgt cgccgccgac catgtcgccg
     1621 tccttcgacc acgcggccca gcgggcgacg agctgggcga ggatcgcggc gcggccggca
     1681 aagccgccct ggagcgcgtc ctcgaggttg agcaggcaca gataattctc gcggcccttg
     1741 cggacgacca cctttcggcg ccgcgcctcg gggtcgggaa acagccgcag cccctcgcgg
     1801 tcgagctggc gctgaagggc cttggtgaag gtcgacaccc acaccgcgcc gtcggcgctc
     1861 tccgcccaca gcgaggcggg ggcgagatat cccagcgtct tgccgatgcc ggtgccggct
     1921 tcggcgagaa gcagattggg ctggccctcg accttgcggg ggtcgaaggc ggcggcggcc
     1981 gcggccgcgt agtcgcgctg gccctcgcgg acctcggcat cgcttccgac ccgccgggcc
     2041 agcggctcga ccgcccgatc ctgcggcacg aggacggtgc gcggcgccgg ccgggcgccg
     2101 ctctcctccc attccggcag cttcgagaaa agccagcgct cgtcgcgctc cggccgcttc
     2161 agccgctgcg cgacggccgg cgcccacgac cagcgcagcc gccacagcgc ctgcgccgag
     2221 gcccaggccc cctcccgctc cggccaatcc cgttcgagcg tggcgagcag ggccgcggcg
     2281 gcacggagca ggaaggaggc cgcctcggag tcgtttgccg gcgcttcgat tcgaagcgcc
     2341 tcggcgagcc ccttgggcgt gggcacggcg aatcgggccg ggtggacgaa ggcgaacagc
     2401 tcgagcaggt cgagccccga cagatcggga tagccgagcc gctggccgac caggggcgcg
     2461 ttgagcatga ccagcggagt ctcggcggcg cgggcgatcg cctcgccgcg cccgagcccg
     2521 cgaacctcgc cgtcggtcga ggcgagccag atgccgccat gggtcgcgtg aagggcggga
     2581 taggggaagg cgccagtcat ggccccatca taggacaggc ggaacgaatg tgaaacggtc
     2641 agcgtcggtg ccgggcgccg atttccggct tcagcgcccc agcgcccgcg ccagcatcgc
     2701 cttcacccgc gccaccagct ccggcgtcct gcgtccgagg aggccgcgcg ccgactcggg
     2761 cagatgcgcg gcgggctcgc catggtcctg gaagacgtag aagtcgaaca gcgcccgcca
     2821 cgccgcccgc tcgcgcggcg gcagatcctt caccgtgatc gccgcgtggt agagggcgtt
     2881 gagcggcgtc agcagcggcg gctcgccgtc gcgccaccag aaattgacca tcgctccgag
     2941 ctcgtcgagc gactcgaccc cgtgccacca caggctcggc agataaagcg cgtcgcccgg
     3001 gccgagctcg gccgtctccg ccgcctccag cgcctcgcgg aagcgcggat ggcgctcgaa
     3061 atcgggggcc tcgagatcga cgaggctggt cggctggccg gcgagggtga agtcgagcgg
     3121 gccgacatag aggttgcgga cctggtcggt ggggaacagg gtgaagcggc ggcgcccggc
     3181 gacgacgcag gcgaggttct gcggcaggtc ccagtgcgcg gcggtgcggg tgcggttgcc
     3241 gagccagagc gagaccagct tctcccgccc cggatcgagc agcggcatcg gcacgtccgc
     3301 cttgagcccg ggcaggtggc gggcgatcgg gatcgcgccg gcatagatgg agaagggatt
     3361 ggggtggccg cgctgccgga gcagcagatc gaggagctgg tcgactgtag ccatccggcg
     3421 gtcgtggttg aagccctgga agtcggccgc atagccgaag cgcccggcga tctcgggcgg
     3481 gccgaaccag gcttcgaacg gctcgtcggc ggcggcgcgg cgaagatagg cggcaagcgc
     3541 ctcgtcgccg tcgcagcggg tcgcgggcca tccggcgcac aggccccgca ggacggcggg
     3601 ccggccggcc gggacgatgt ccctctcgaa ccgctcgcga tcgacgtcgt gatattccgg
     3661 aacggggcgc acgacccgca gcgctagcag agccccgccg cctccgctag gagcgcgcca
     3721 tgagcgagaa ggaagcgatc cgagcggcgg ccctggcgtc gaaggcctgg ccctatgagg
     3781 aggcgcgcaa gctcctgaag cgctacccgg acgggccgcc ggcgggcgag atcgtcttcg
     3841 agaccggcta cgggccgagc ggccttccgc acctgggcac tttccaggag gtggcccgca
     3901 ctctgatggt tcgccacgcc ctcgccgaga tggtcgactg gccgagccgc ctgatcgcct
     3961 tctccgacga catggacgga atgcgcaagg tgcccccggg cgtgcccggc caggacatga
     4021 tgcacgccca tctcgaccgg ccgctcagcc tggtgccgga cccctatggc tgcagccacg
     4081 acagctatgc ggcgcacaac aacctcctgc tgcgccaatt cctcgatcgc ttcggcttcg
     4141 actacgagtt cctcgcctcc tcggactgct accggtccgg ccgcttcgac gagaccctcc
     4201 gccaggtgct ccgccgctat gactcgatca tggcggtgat gctcccgacg ctcggcgagg
     4261 agcgccgccg gacctattcg ccggtccttc cgatcagtcc ggaaagcggc aaggtgctcc
     4321 aggtgccgat cgaggtagtg gacgcggagg cggggacggt ccgctacacc gatccggcca
     4381 ccggccaggc ggtggagcaa tcgatcctct ccggcggcgc caagctgcag tggaaggtcg
     4441 actgggcgat gcgctgggcg gcgctcggcg tcgattacga gatgtcgggc aaggacctga
     4501 tcgattcggt cgtccagagc tcgaagatcg cccggatcct cggcgcccgg ccgcccgaag
     4561 gcttcaacta tgagctcttc ctcgacgaga atggcgagaa aatctcgaag acccggggca
     4621 acggcgtcac cctcgacgaa tggctgacct acgcgcccga ggagagcctc gtcttctacc
     4681 tctaccgcga gccgaggaag gccaagcagc tgagcttcgg gatcatcccc aaggcggtgg
     4741 acgaatatca ccagttcctc gccgcctatg cggaccagga atgggataag cgcctcggca
     4801 atccggtcca ccacgtccat gccggcaagg tgcccgaggc acgcatgccg ctcagcttcg
     4861 cgcttctcct caacctggtc ggggtcgcct cgaccgacga caaggacctg ctgtggggct
     4921 tcgtgaagcg ctacgccccc gaagcgtcgc cggaaaccca ccccgagctc gacgccctga
     4981 tcggccatgc cttggcctat ttccgcgact tcgtcgccgg ctcgctgcgc cgccgggcgc
     5041 ccgacgagcg cgaggcggcg gcgctccggg cgctcgactc gaagctcgcg gcgatgccgg
     5101 aggacgcttc cgccgaggac atccagaccg aggtctacga ggtcggcaag gcggccggct
     5161 tcgacaatct gcgcgactgg ttccgggcga tctacgagac cctgctcggc accagccagg
     5221 gcccccgcat gggcagcttc gtcgcgctct atggcatcgc caacagccgc cgcctgatcg
     5281 ccgaggccct ggcctcggcc tgatccttcg ccgggatgag gggatcagga acgcagatgt
     5341 tcctgtcgcc ccaccgcata ggcttcgcgg gcgaagccgc gcagcccgtc cacctgctcg
     5401 acgcgctcga ggcggaaatg gtggacgtgg ttgcggggcg agatcgtctc gaccttgctg
     5461 aagagcggat cctcgagccg ccgggccagc acgaaatggc cgaggaccca gcggcgacgg
     5521 acggtcagct gcgcgaagga gatccgggcc tggaaggcga tccgggtctt ttcgggaagc
     5581 accgtcaccg gccccatttc ttcgagcatg gcgaggaagg cgtcgtagat gtcgcggacg
     5641 aggggaggct tgccggcgaa atgaccgtcg agcgcgtggc ggccgcaggc gtgcgactgg
     5701 ttggcgttgg cgaaggcccg gccgcaggcg gggcaggtcc agagcggttt gcgggcgcgc
     5761 atcggcggcc ctcctacacc gttggacgag cccgggcgaa tgggcgtatg gggccggcca
     5821 tgaaccccgc cggccacgcc atcgagatca cgccgttcgg cggacgcgtg cgggtgcgcg
     5881 tcgccggcga gatgctcgcc gacaccgccc atgccctcac gctcgaggaa ggctcgctgc
     5941 cgccggtctt ctacatcccg cgggcggacg tccacatgga ccggctgtcg ccgagcgcca
     6001 cgacgagcca ctgtccgttc aagggcgacg cgtcctattt cagcgccgcg tccggcccgg
     6061 cggacgtcgc ctggagctat gaggcgccgc tcgcccaagc ctcggcgatc gccggccatc
     6121 tcgctttcta tccggaccag gccgagatcg agaccgtgcc tttcgggtag cgcgtcagcg
     6181 cggggagcga agcgacgaag cagtcctgcg cggcttctga gtcagagctg gattgcttcg
     6241 cttcgctcgc attgacgggg caggacctca tcccttcttc gccgcgatcc agtcgtcgat
     6301 cttcttctcg agcacggcga gcggcagggc gccggtcatc agcacctggg cgtggaactc
     6361 gcggatgtcg aagcgcgggc cgagcgcctt ttccgcccgc gccctcagct cgctgatctt
     6421 gagctggccg atcttgtagg ccagcgcctg gccggggatg gcgatgtagc gctccgtctc
     6481 ggcggtggcg ttgctccggc cgcgcgagct gttgtcgagg atgtactgga tggtctggtc
     6541 gcgggtccat cctttcgagt ggatgccggt gtcgacgacg agccggatgg cgcggaacag
     6601 ctgcgaatcg agatagccga aatattggta cgggtcggta tagactccga gctcgcggcc
     6661 cagcgtctcg gcgtagaggc cccaaccctc gacataggcg gtgttgccgc cgaagcgcat
     6721 gaagctgggc agggcttcgt tctcctgggc gaggctgatc tggaaatggt gcccgggcgc
     6781 gccctcgtgg agataaaggg tctccatccc gggcgtggtg cgggacggca gatcgtaggc
     6841 gttgaaatag aagacgccgg gccgcgaccc gtcggtcgtt ccgctctggt aggagccgcg
     6901 cgccgcgccc ttctcggtga gcgcgggcac cggccggatc tcgaggcggg tcttggggat
     6961 ggtcgagaac atcgtcggca gggtcgcgtc gaggcgcttg ccgatcgcga catagccctg
     7021 ctggagcgcc tcgcgcgagg ccggcttgaa cctggggtcg gtgcggatgt gctcgaagaa
     7081 ctgctggagc gttccggtga agccgacccg cgccttcacc gcctccatct cggcgtgaag
     7141 gcgcgccacc tccgccaggc cgatccggtg gatctcgtcg ggcgtcatgt cggtcgtggt
     7201 gctcagcgcc accagatgcc ggtagagcgc cggtccgccc ttcatgtcct ggaggccgac
     7261 gctgtcgcgg gcccgcggca ggtagcggtc acggatgaag tcgcgcagcc gggtgacggc
     7321 gggccggatc ttcgtcgaga tcgcctcggc ataggccgcc ttcagccggg gccggtccgc
     7381 ctcgggcacc tccgccggga aattggccac gggccggtag aggctgctcc cttccacccc
     7441 ttccgccagc atcgcgtcga gctgctcgac gacattggcc atgaccagct tgggctgcac
     7501 caccccggcg gccagccctt gctccatccg cgcgatcgcg ccgtcggcga gcgagacgaa
     7561 gtcgtcgaac cggctgaggc cgttctcata atccttgagg gtcttgtagg gcgcgatccc
     7621 ttcgcccgag tgggtctcca cgaagaaggt gtggaagccg ttgaaatggt cgatcggccg
     7681 gacgacgctg gcggcgaata tctccccctc gagccccttg agatccatgc cgcgctgcca
     7741 cttgaacacg tcgtaggcga cgcgttcgtc ggcgccgagc gcatcgcggt cgatctgcgc
     7801 cagcctggca agctccgccc gcgccgcggc acgctccgcg gcgtaatagg agtcgctgaa
     7861 gaaatccccg aatcggtcgg catagcgcag gtcgccgcgg gcgagcgcct ggatcggatt
     7921 gcgctgaagg ccggcctcgt cgctgtcgcg gaacagcgcc gccagcgcct gggacgctgt
     7981 ctgacgaggg gccggggccg cggcggcttc aggcgccggc gaagcctggg aggaggcgca
     8041 gccggcgagc aacgccgccg tcagtccgat cgatagggat cctcgcatga ccgcatcctt
     8101 cctgagtctc gccggtgatg cgcggctcta gagcgattgc gcggtcattg cgagcgcgag
     8161 ggatcaggcc ttcttggatg cgatccagtc gtcgatcttc ttctcgagca cgcccatcgg
     8221 cagcgagccg gtcatcagca cctgggcgtg gaactcgcgc gggtcgaacc tggcgccgag
     8281 ccccttttgc gccttggcct tgagccgctg gatggtcagc gctcccacct tgtaggccag
     8341 cgcctggctg gggatggcga tgtagcgctc gacctcagag gtcacctcgg tccggctcat
     8401 gccgctgttg gcgagcatgt agtcgatcgc ctgatcgcgg ctccacccct tggcgtggat
     8461 gcccgaatcg acgacgaggc gcatcgcccg cagcatctcg tcgttgaggg cgccgaagcg
     8521 ctgataggga tcggtctcca tgccgagctc gtcccacagc gtctcggcat agagcgccca
     8581 gccttcgaca taggcggtgt tgccgccgaa gcgcatgaag gcggggagcg cctcattctc
     8641 ctgggcgagg ctgatctgga aatggtggcc cgggctgccc tcgtgcaggt agagcgtctc
     8701 catcccccag agacggcgcg aggggaggtc gtagctgttg tagtagaaga tgccgggacg
     8761 ggacccgtcg ggcgtgccct gctggtagga gccgccggct tccgtcttct cgcggaaggg
     8821 ctcgaccgga cggatctcga gggggctctt ggggatggtc gagaattgct cgcggatccg
     8881 ggcgtcgacc cgccgcgcga tcgcctcata gccctcgcgc agcgcctcct tgctggccgg
     8941 cttgaatttc ggatcggtgc gcagatactc gaagaactgg ggcagggtgc ccttgaagcc
     9001 ggcgcgcgtc ttgaccgcct ccatctcgct cttgatccgg gcgacctcgg agaggccggt
     9061 ggagtggacc tcgtccgcgg tcagcggcag cgtcgtcgtg ctctcgatca gtcgccggta
     9121 gacttccggt ccgcccttca tgtgcaccag cccgacgcct tcgcgcgcct tgggcaggta
     9181 ctccgtcttc aggaagtcgc ggacgcgccg gtggaccgga tagatttcgc tctcgatcga
     9241 cgcccggtag gcggcggcga gacgcgtctt gtcggcttcg gagaaatcgg ccggaaagcg
     9301 cttgaccgga ccgtagaagg gggagtcctc gaccttctgg tcgagcagga gggtgagctg
     9361 gtcgatcacg ttctgggtcg tcagcttggt ctcgaacacg cccgattcca tgccctcgcg
     9421 gaagcggccg atcgcgcggt cgtggaagag gacatagtcc ttgtggcgct tcaaattgtt
     9481 ctcatagtcg gcgaccgtct tgaacggcgc cgccccctcg cccgaggcga aggcggggta
     9541 gaaggtgtgg aagccggaga aatggttgat cggccggacg atgccgagcg cgagcatgcg
     9601 cggctcaaga ttcttgagcg cgtcccgggt ctgccactcg aagacgtcgt aggcgatccg
     9661 gtcggtctcg ctcagcttcg agcggtcgat ccggcgcaga gcggcgagat cgccctccga
     9721 cgcggcacgt tcggcattga aataggcgtc ggtgatgaag tcgccgagcc ggtccgcata
     9781 gcgcaggtcg ccgcggaaga tggcgcccac cgggttgcgc ttgagattgg cctcgtcgct
     9841 ctcgtggaag aggcgcgcga gcttcgcgcc ctcgctctcg gcggcggcgg gagcggcggg
     9901 cgccgaggcg ccgccctgcg ccgcgagcgg cgcggaaacg gcggcgagcg agacggcggc
     9961 gagcaggaag aacgaaggtt tcatgagggc ctccccgtgg agctgtgttg gcgaattaag
    10021 acggcttgag acgctgcggc aagctgccga tcttgctcaa taataagcgg tcggcttgac
    10081 gttctgctcg ccgtaatagc cccacggccg ttcccgatac catttggtga tgacgtattt
    10141 ggtgccggcc tcgacggggc agccggtgtg gagcgagtag gtgttgggct cgccatattg
    10201 gtcgaggttg ttccacagca gcaggttgcc ggtgcgcgga gtcacccgga cgttggcctt
    10261 ggggaagaag gtctgcccgc cggcctcggg ctcgttgagg aagatcatcg cggtccaggt
    10321 gcgctggccg ccctgacgct ccatctccgg ccaataaggc tggttggtgt agaagaaatc
    10381 gtggtgcggc ttgaactgct ggccgaccgc gtagcgctgg ccctggatgg tctcgccgtg
    10441 gatggggtcg attccggtga ggcggttgat cttcgcctcc accatctgat tggcgcggtc
    10501 ctcgagccgc agattgcagc tctcgctggt gcggaactcc gggtccgggc tgggcgcgag
    10561 caggccggag ggaattcggt ccttgtcgat cagctcgatg acctcgccgc attcggcggg
    10621 cgtcaggaac tggcgcacga cgaagatgtc gaggccctgg gcggggatct tgaacgcgtt
    10681 gggagtcttg aggacgcgct cgcgcacata ggtg
//
LOCUS       DASVQZ010000111         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_122322, whole genome shotgun sequence.
ACCESSION   DASVQZ010000111 DASVQZ010000000
VERSION     DASVQZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122322"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VF547_03055"
     CDS             <1..264
                     /locus_tag="VF547_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261834.1"
                     /translation="VAAALALAFTAKRHGRPFFLAAALVAASMVLFETVGRWPAWHSL
                     FARFAGVPLAPVIAVAAAAGCAIAWAGWTAGSVKARGPAAQPA"
     gene            complement(290..913)
                     /gene="cmk"
                     /locus_tag="VF547_03060"
     CDS             complement(290..913)
                     /gene="cmk"
                     /locus_tag="VF547_03060"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX8261835.1"
                     /translation="MIVAVDGPAASGKGTIARALARHYGLPHMDTGMLYRAAALNLLN
                     LGLDPESEFQAARACDVSQIAPDDPELRSEAVGSIASRISAYPLVRAALIERQRHFAA
                     QAGGAVLDGRDIGTVIAPHADAKLFVTASPEVRARRRADELHARGLSVHYEEVLADIR
                     ARDERDSARAAAPLVKAADADLLDTSDLGVEAAIAQAIRLVGSRVGR"
     gene            complement(910..2202)
                     /gene="aroA"
                     /locus_tag="VF547_03065"
     CDS             complement(910..2202)
                     /gene="aroA"
                     /locus_tag="VF547_03065"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX8261836.1"
                     /translation="MEFGPAGPLRGTARVPGDKSISHRALMISALAVGRSRIEGLSRG
                     EDVAATARAMQAMGGSVHWRGDGSVEVDGVGVGALLQPEGALDMGNSGTSARLLMGLV
                     ATHPIQATFVGDASLSRRPMERVAAPLRRTGAEFLASPGGTLPLTVRGIAPAVPLATR
                     LEIASAQVKSALLLAGLNTPGITRVTELVPTRDHSERMLTLFGADLRVEGPDIVLRGE
                     TELCPQRLRIPGDPSAAAFLAVAASIVPGSDILVENVGINPHRIGFYKVLAEMGADIA
                     FENAREASGEPVADLRVRHAPLRAVDVPPELAPSMIDEFPIFFIAAAFAQGRSTATGL
                     HELRVKESDRLSAMADGLRAVGAGVEEAESSIAVHGTGGDPLPGGGAPVATRLDHRIA
                     MSFAVAALHCRAPVALDDFAPAATSFPGFAALLRSLQP"
     gene            2350..2676
                     /locus_tag="VF547_03070"
     CDS             2350..2676
                     /locus_tag="VF547_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX8261837.1"
                     /translation="MVKAEWGTKRTCPKCGNRFYDLGKEDPVTCIACGIAWDPEPVLK
                     SKQPLPYDEPAKAVADKPEAEDADLAADELAIDEEAEENPDDEVDLGGDDDIGVPGAG
                     GDDEEV"
     gene            2812..2887
                     /locus_tag="VF547_03075"
     tRNA            2812..2887
                     /locus_tag="VF547_03075"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2845..2847,aa:Ala,seq:ggc)
     gene            complement(3130..>3397)
                     /locus_tag="VF547_03080"
     CDS             complement(3130..>3397)
                     /locus_tag="VF547_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261838.1"
                     /translation="IYNFQTGIDKVDLSRIDSNVFLEGDQAFTFRTGEFSGQGAASAG
                     EVRVITSIPGSFYALEADVNGDGSADLYILFDHAVDLSPADIIY"
BASE COUNT          502 a         1051 c         1362 g          482 t
ORIGIN      
        1 gtcgccgccg cgctggcgct ggcgttcacg gcaaagcggc acggccggcc cttcttcctc
       61 gccgccgctc tggtcgcggc gagcatggtg ctgttcgaga cggtggggcg ctggccggcc
      121 tggcattccc tgttcgcgcg cttcgccggc gtgccgctcg cgccggtcat cgccgtggcg
      181 gcggcggcag gctgcgcaat cgcctgggcg ggctggacgg cgggaagtgt gaaggcgagg
      241 gggcctgccg cccagccggc ctgaggatgg acggcgcctg cgagccgggc tagcggccga
      301 cgcgcgatcc gacgaggcgg atggcctggg ctatggcggc ttcgacgccg aggtcgctgg
      361 tgtcgaggag atcggcgtcg gcggctttga cgagcggtgc ggcggcgcgg gcgctgtcgc
      421 gttcgtcgcg ggcgcggatg tcggcgagaa cttcttcata gtggaccgag aggccgcggg
      481 cgtggagctc gtcggcgcgg cggcgggcgc gcacttccgg agaggcggtg acgaagagct
      541 tggcgtcggc gtgcggcgcg atgaccgtgc cgatgtcgcg gccgtcgagg acggcgccgc
      601 cggcctgcgc ggcgaagtgg cgctggcgct cgatcaaagc ggcgcggacc aggggatagg
      661 cggaaatgcg gctggcgatg ctgcccaccg cttcggagcg cagctcgggg tcatcgggtg
      721 cgatctgcga gacgtcgcag gcgcgggcgg cctggaactc gctttcggga tcgaggccga
      781 ggttgaggag gttgagggct gcggcgcggt agagcatgcc ggtatccatg tgcggcaggc
      841 cgtaatggcg ggcgagggcg cgggcgatgg tgcccttgcc gctcgcggcg gggccgtcga
      901 cggcgacgat catggctgga ggctcctcag aagggcggcg aaaccgggga agctggtggc
      961 ggcgggggcg aagtcgtcga gggcgacggg ggcgcggcag tgcagggcgg cgacggcgaa
     1021 gctcatcgcg atccggtggt cgaggcgggt ggcgaccggc gcgccgccgc cgggcagggg
     1081 atcgccgccg gtgccgtgga cggcgatcga ggactcggcc tcctccacgc cggcgccgac
     1141 ggcgcgcagc ccgtcagcca tggcggagag gcgatcggat tccttgacgc gcagctcatg
     1201 gaggccggtc gcggtgctgc gcccttgggc aaaggcggcg gcgatgaaga aaatcggaaa
     1261 ttcgtcgatc atcgaaggcg cgagttcggg cggcacgtcg acggcgcgca agggggcgtg
     1321 gcggacgcgc aggtccgcga ccggttcgcc ggacgcctcg cgcgcgttct cgaacgcaat
     1381 gtcggcgccc atttcggcaa gcaccttgta gaagccgatg cggtgcgggt tgatgccgac
     1441 attctcgacg aggatgtcgg agcccgggac gatcgaggcg gcgacggcga ggaaggcggc
     1501 ggcggacggg tcgccgggga tgcggagccg ctgggggcag agctccgttt cgccgcgcag
     1561 gacgatgtcg gggccttcga cgcggagatc ggcgccgaac agcgtcagca tgcgctcgct
     1621 gtgatcgcgg gtggggacga gctcggtgac gcgggtgatg ccgggcgtgt tgaggccggc
     1681 gaggaggagg gcggacttga cctgtgcgga ggcgatctcg aggcgggtgg cgagcggcac
     1741 ggcgggggcg atgccgcgga cggtcagggg gagggtgccg cccgggctcg ccaggaactc
     1801 ggcgccggtg cggcgcaggg gcgcggcgac gcgctccatc gggcggcgcg aaagcgaggc
     1861 gtcgccgacg aaggtcgcct ggatcgggtg ggtcgcgacg aggcccatga ggaggcgggc
     1921 ggaggtgccg ctattgccca tgtcgagggc gccctcgggc tggaggaggg cgccgacgcc
     1981 gacgccgtcg acctccacgc tgccgtcccc gcgccaatga accgagccgc ccatcgcctg
     2041 catggcgcgg gcggtggcgg cgacgtcctc gccgcggctc aggccctcga tccggctgcg
     2101 gccgacggcc agtgcggaga tcatgagggc gcggtgcgag atggacttgt cgccggggac
     2161 gcgggcggtg ccgcgcaacg ggcctgcggg cccgaactcc atcctgtcgg gggccggagg
     2221 ggcttggggg gcggcgcctt ccatgcgcgc ggcttttgac agtggcccca gcctatggca
     2281 aggcgcggcc aacgaggcgg ccccgccggg cgccgcccct ttttcacttt tatcggagac
     2341 gtttcaccca tggtgaaggc tgaatggggc accaagcgca cttgtcccaa atgcggcaac
     2401 cgcttttacg acctcggcaa agaggacccg gtgacctgca tcgcttgcgg catcgcctgg
     2461 gacccggagc ccgtgctgaa atcgaagcag ccgcttccct atgacgagcc cgccaaggcc
     2521 gtcgccgaca agccggaagc ggaagacgcc gatctcgccg cggacgagct ggcgatcgac
     2581 gaggaagccg aggaaaatcc ggacgacgag gtcgatctcg gcggcgacga cgatatcggc
     2641 gttcccggcg ccggcgggga cgacgaagag gtctgacgcg cagcgtccgc gccccggtcc
     2701 gtgcggagac cgggcgcgcg gggcgcctca gatcaaacag ccttgcgcct gagcggatgc
     2761 tcacttaaag gcgcggcaca ccggccctcc caggggcggt acgaagggtg cggggccgta
     2821 gctcagctgg gagagcgctg caatggcatt gcagaggtca ggggttcgat ccccctcggc
     2881 tccaccatcc gcaaccccat tgaatcggtc gttctcggga cggcttgccg acccgagagt
     2941 cgcctgaacg cggcgttcgg atcctgcgcg agcgcaagtg gacgacggta tggctcgtga
     3001 actccgccag aaggtatcga tcgcggccga ggtcggccgc cgggtgctcg tcattgcgcg
     3061 ccgacatgtc ccgcgccccc gccgacggga ggaacgtacc ttcagccggc gggatggtga
     3121 gtccacaccc tagtatatga tgtccgccgg gctcaggtcg acggcatggt cgaagagaat
     3181 atagaggtcg gccgagccat ccccgttcac atcggcctcg agagcataga agcttccggg
     3241 aatgctggtg atgacgcgca cttctccggc tgaggctgcg ccctgaccgc tgaactcgcc
     3301 tgtgcggaag gtgaaggcct ggtctccctc caggaacacg ttggagtcga tccgcgacag
     3361 atccactttg tcgatgccgg tctggaagtt gtagatc
//
LOCUS       DASVQZ010000112         8204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_891582, whole genome shotgun sequence.
ACCESSION   DASVQZ010000112 DASVQZ010000000
VERSION     DASVQZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891582"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1814
                     /locus_tag="VF547_03085"
     CDS             <1..1814
                     /locus_tag="VF547_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8261839.1"
                     /translation="LLRDWVKARFGRRAEAMDRGVDKDGAFYLLSLRLIPAFPFFLVN
                     LAMGLTAMGLVTFALVSQAGMLLGTIVYVNAGTQLAAIESTSDILSPGLIGSFVLLGL
                     FPLAAKAMVAAWKRRRIYRRWRRPRRFDRNLIVIGAGAGGLVTALIGATVRAKVTLIE
                     KGEMGGDCLNTGCVPSKALIRSARAAHEVRTAAAYGVAPAEPEVRFAEVIERIHRIIA
                     EIAPADSVERYSGLGVDVRKGHARLVDPWTVEIDGGERLTARSIVIAAGGEPAVPDIP
                     GLAECGYLTSDSMWDALRRRERVPGRIAIVGGGPIGTEMAQAFARLGAKVTQIEHGPR
                     LLPREDEEVSGLVEQVLREEGVDVRTGCEAVRVDGKTLVVRRDGAAGEIPFDEIVVAV
                     GRKARLTGYGLEALGIETGRTIVTNEWLETLYPNIYAVGDVAGPYQFTHFAAHQAWFA
                     AVNGLFGQFRRFQADYRVLPWVTFTDPEVGHVGHNELSAREAGIAVEIVRYDLGHLDR
                     ALAEGASRGFVKLLVQPGKDRLLGATIVADRAGELLAELVLAMKHGLGLNRILGTIHA
                     YPTLAEANKYAAGEWKKAHKPERLLAWIERYHRWRRG"
     gene            1811..2374
                     /locus_tag="VF547_03090"
     CDS             1811..2374
                     /locus_tag="VF547_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX8261840.1"
                     /translation="MTRIVILAKQPLPGRVKTRLIPALGPEGAARMAAQMLCETVNEA
                     LATGWEVELWGEPDPAGWYEGPPLRLRAQGSGGLGARLAQATRQTLDEASSILLIGTD
                     CPELDRSRLAAAAAALGPYDAVIHPTEDGGYALLGLKRFSATLFEDMPWSTATVFDQT
                     RARVEALGWSLLVGDTLRDIDEPEDLR"
     gene            2544..2942
                     /locus_tag="VF547_03095"
     CDS             2544..2942
                     /locus_tag="VF547_03095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8261841.1"
                     /translation="MPTPLPPYAELLGLRTERGADGALLWVMPFREEVVGRPGFLHGG
                     AIAGLLEFAAFGTLYEALGPGASASPINVSVDFMRGGTDHDTFASAVVTRLGSRVANV
                     EAHAWQQDRDKPIAAARMNLMVRRAPSLRA"
     gene            complement(3012..4154)
                     /locus_tag="VF547_03100"
     CDS             complement(3012..4154)
                     /locus_tag="VF547_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8261842.1"
                     /translation="MPLDPESFDLLLAAVRRFVRERLRPLEERVDREDEVPAAIVAEM
                     REMGLFGLSIPEEYGGLGLTMSEEVRVALELGGTTPAFRSTFGTNVGIGSQGLVMAGS
                     EAQKAQWLPRIANGEVITSFALTEPGAGSDSASVQTKAVREGEVYRLTGTKRFITNAD
                     KASLFTVMARTGEPGAKGVSAFLVPADLPGVSVGKPEKKMGQQGAHVCDVNFDGAPVP
                     AANRLGEEGEGFRIAMRVLDRGRLHIAAVCVGIAERLIADAVAYAAERRQFGRPIAEF
                     QLIQAMIADSKTETMAAKALVLEAAALKDKGEPITLEAAAAKYFASEAVGRVADRAVQ
                     IFGGAGYIADYGIERLYRDVRLFRIYEGTSQVQQLVIAREVMRRGG"
     gene            complement(4285..5400)
                     /locus_tag="VF547_03105"
     CDS             complement(4285..5400)
                     /locus_tag="VF547_03105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8261843.1"
                     /translation="MPLYLTEDQAMLRDTARELMADEGSIANQLRKYRDSGCGDGFGH
                     ALWRQMAELGFSGILIGESDGGLGLGHVEAGIVLEEIGRNLTPSPFLITAVAVVEALK
                     GTPHGGRWFPGILAGQTVAALAVDEGPKHRPEAIAMRAERAGNGFRLTGRKQFVVQGA
                     SADVTVVAARTDEGLTLFAVEKDSKGLEVEGVRLVDSSIGARLDFQGVEVDADSVIGE
                     VGGGEAVLARVLAAGRAGAASEMVGLGSAAMAMTVDYLKQRKQFGRLIGEFQALQHRA
                     AHLYSELEIARSAAIKAQQLLDEGSPEAETMVSVAKAKAGRASTLAVQEGVQMHGGIG
                     MTDEYDIGLYMKRDRALNELFGDPNYHAERVARMKGY"
     gene            complement(5510..6682)
                     /locus_tag="VF547_03110"
     CDS             complement(5510..6682)
                     /locus_tag="VF547_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8261844.1"
                     /translation="MSEIAELAAFREEVRAWLEANCPPEMREPMRDESDACWGGRNPV
                     FKNDAQKLWMERMAERGWTVPDWPRDYGGGGLSPAETKVLKQEMARIGARNPLMSFGI
                     SMLGPALLKYGTEAQKKRFLPESARGEIRWCQGYSEPNAGSDLASLQTRCEDMGDHWL
                     VNGQKVWTSYADKADWIFCLVRTDFQAPKHKGISFLLFDMATPGVSTKPILLISGSSP
                     FCETFFDDVKVPKDQVVGEVNKGWDVAKYLLGHEREMISGMGLGGQTASLGEAFADVV
                     ANDPVLRADMARFDVDALAFRAMSERFIDELKAGMAHPAQPSMMKYAGTELNKARHEL
                     VMAAGGSDSLEWESDRSRKGKAAREWLRTKANSIEGGTSEVQLGIIAKRILDLPGA"
     gene            6743..7219
                     /locus_tag="VF547_03115"
     CDS             6743..7219
                     /locus_tag="VF547_03115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8261845.1"
                     /translation="MRTALLAAAAAIGPATGASAAERPAVEYYAPDEAAQAAARAGGF
                     RLPFSSAVRVGDVLYLSGVIGTRDDGALPPGIEAQARQTMDNIGAMLKRAGRSFDDLF
                     KCTVMIDDMKQWQAFNQVYTTYFKPDRLPARSALGADGLALGALVEVECLAHAPAR"
     gene            7333..>8204
                     /locus_tag="VF547_03120"
     CDS             7333..>8204
                     /locus_tag="VF547_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261846.1"
                     /translation="MLVRRIKRASALLLAGPAAVASAQAPQAAPPALKAPPPPSEERV
                     SKADIPAGQRYLMDRHKISAAEAAERIGLEEEVSRLANQLRTSDPDDFGDLWIEHEPV
                     YRIVVAFLNNEDRTQLRESIAPNLRRYVQFKQVKTSWKQREALVERIVAALKPLAIEY
                     YSHYDHRTDSVVIQTQTDRAVQQIRQAIPNDLQSFVTIQRGRVPQKAQATGARAGDYL
                     HGGWWYYLSNAGGNWHECSFAFNAKDNQNRPVILSAGHCSHPTQVYYSDHWVSLATPF
                     QDHEEGKYDYQAQL"
BASE COUNT         1251 a         2893 c         2753 g         1307 t
ORIGIN      
        1 acctgttgcg ggattgggtg aaggcgcggt tcggccgccg cgccgaggcg atggaccgcg
       61 gcgtcgacaa ggacggcgca ttctatctcc taagcctgcg gctgatcccg gctttcccct
      121 tcttcctcgt gaacctggcg atggggctga cggcaatggg gctggtcacc ttcgcgctgg
      181 tcagccaggc cggaatgctg ctcgggacga tcgtctacgt caatgcgggg actcagctgg
      241 cggcgatcga gagcacgagc gacatcctct cgccgggact gatcggatcg ttcgtcctgc
      301 tcggcctgtt cccgctggcc gccaaggcga tggtcgcggc gtggaagcgc cggcgaatct
      361 accggagatg gcggcggccg cgccgcttcg accgcaacct gatcgtgatc ggcgccggcg
      421 ccggcggcct cgtcaccgcg ctgatcggag cgacggtcag ggcgaaggtc acgctgatcg
      481 agaagggcga gatgggcggc gactgcctca acaccggctg cgtcccctcc aaggccttga
      541 tccggagcgc ccgcgccgcc cacgaggtcc ggaccgcggc ggcctatgga gtcgcgccgg
      601 ccgagccgga ggtccggttc gccgaggtga tcgagcgaat ccaccgaatc atcgccgaga
      661 tcgctccggc cgacagcgtc gagcgctata gcgggctcgg cgtggacgtc cgcaagggcc
      721 atgcccggct cgtcgatccc tggacggtcg agatcgacgg cggcgaacgg ctgaccgcgc
      781 ggtcgatcgt gatcgcggcg ggcggcgagc cggcggttcc ggacattccg ggactggcgg
      841 agtgcgggta cctcaccagc gattcgatgt gggacgcgct gcgccgccgc gagcgcgtgc
      901 ccggccggat cgcgatcgtc ggcggcggcc cgatcggcac cgagatggcg caggccttcg
      961 cccgcctcgg ggcgaaggtc acccagatcg agcacggccc gcggctgctg ccgagggagg
     1021 acgaggaggt ctccggcctg gtcgagcagg tccttcgcga ggagggggtg gacgtccgca
     1081 ccggctgcga ggcggtgcgg gtggacggca agacgctggt cgtgcggcgc gacggggcgg
     1141 ccggcgaaat cccgttcgac gagatcgtcg tcgccgtcgg ccgcaaggcg cggctgaccg
     1201 gctacggcct cgaggcgctc ggcatcgaga ccggccggac gatcgtgacc aacgagtggc
     1261 tggaaacgct ctatcccaac atctatgcgg tcggcgacgt ggccggaccc tatcaattca
     1321 cccatttcgc cgctcaccag gcgtggttcg cggccgtcaa cggcctgttc gggcagttcc
     1381 gccgcttcca ggccgactat cgggtgctgc cctgggtcac cttcaccgat cccgaggtcg
     1441 gccatgtcgg ccataacgag ctgtcggcga gggaggcggg gatcgccgtc gagatcgtcc
     1501 gctacgacct cggccatctc gaccgcgccc tggccgaggg cgcgagccgc ggcttcgtga
     1561 agctgctcgt ccagccgggc aaggaccggc tgctcggcgc caccatcgtc gccgaccgcg
     1621 ccggcgagct gctcgccgag ctcgtcctgg cgatgaagca cggcctcggc ctcaacagga
     1681 tcctgggcac gatccacgcc tatccgacgc tcgccgaggc caacaaatat gccgccggcg
     1741 aatggaagaa ggcgcacaag ccggagcggc ttctcgcctg gatcgagcgc taccatcgat
     1801 ggcggcgcgg atgacccgga tcgtgatctt ggccaagcag ccgctgccgg gccgggtcaa
     1861 gacccgcctg atcccggcgc tggggccgga gggggcggcg cggatggcgg cgcaaatgct
     1921 gtgcgagacc gtgaacgagg cgctcgcgac cggatgggag gtcgagctgt ggggcgagcc
     1981 cgacccggcc ggctggtacg aaggcccccc gctgcggctt cgggcgcagg gatccggcgg
     2041 gcttggcgcc cggctcgcgc aggcgacccg gcagacgctg gacgaggcct cctcgatcct
     2101 gctcatcggc accgactgcc ccgagctcga ccgctcgcgc ctcgccgcgg cggccgccgc
     2161 gctcggcccc tatgacgccg tcatccaccc taccgaggac ggcggatatg cgctgctcgg
     2221 gctgaagcgc ttctccgcga cgctcttcga ggacatgccg tggagcacgg cgaccgtctt
     2281 cgaccagacc cgggcgcgag tcgaggcgct gggctggtcg ctcctcgtcg gcgacacctt
     2341 gcgcgacatc gacgagcccg aggacctgcg ctagaccgtg ctggatttag ctttatccgc
     2401 tcatcctggt agggactgag cgaaggcgaa ggcccgaatc gaagcgcgtc cttcgattcg
     2461 ccgcttcgac ttcgctcagc ggctgctcag gatgagcgga agcggttcaa cggaatccct
     2521 caacgctcca ggtccgctcc gccatgccca cccccctgcc cccctatgcc gagctgctcg
     2581 gcctgcgcac cgagcgcggc gcggacggcg cgctgctttg ggtgatgccg ttccgcgagg
     2641 aggtggtcgg ccggcccggc ttcctccacg gcggagcgat cgcgggactg ctcgagttcg
     2701 ccgccttcgg gacgctctac gaagcgctcg gccccggcgc gagcgcgagc ccgatcaacg
     2761 tcagcgtcga cttcatgcgc ggcggcaccg accacgacac cttcgcctcg gcggtggtga
     2821 cccgcctcgg cagccgggtg gcgaacgtcg aggcccatgc ctggcagcag gaccgggaca
     2881 agccgatcgc cgcggctcgg atgaatttga tggttcggag ggcgccgtca ttgcgagcgt
     2941 agcgaagcaa tccagcggca ccaaggccgg gaactggatt gcttcgtcgc ttcgctcctc
     3001 gcaatgacgg gtcacccgcc ccttctcatc acttccctcg cgatgacgag ctgctggacc
     3061 tggctcgtcc cctcgtagat ccggaacagg cggacgtcgc ggtagaggcg ctcgatcccg
     3121 taatcggcga tgtagccggc gccgccgaag atctggaccg cgcggtcggc gacgcgcccg
     3181 acagcctcgc tggcgaaata tttggccgcc gccgcctcca gggtgatcgg ctcgcccttg
     3241 tccttgagcg ccgccgcctc cagcaccagc gccttcgccg ccatcgtctc ggtcttcgaa
     3301 tcggcgatca tcgcctggat cagctggaac tcggcgatcg gcctgccgaa ctgcctgcgc
     3361 tcggccgcat aggcgacggc gtcggcgatc agccgctcgg cgattccgac gcagaccgcg
     3421 gcgatgtgca gccggccgcg gtcgagcacc cgcatcgcga tccggaaccc ctccccttcc
     3481 tcgccgaggc ggttggcggc cggaaccggc gctccgtcga aattgacgtc gcagacatgg
     3541 gcgccctgct ggcccatctt cttctcgggc ttgccgaccg agacgccggg aaggtcggcg
     3601 ggcaccagga aggcggagac ccccttcgcg ccgggctcgc cggtccgggc catgacggtg
     3661 aacagcgagg ccttgtcggc attggtgatg aagcgcttgg ttccggtgag ccggtagacc
     3721 tcgccctcgc gcaccgcctt ggtctggacc gaggcgctgt cggagccggc gccgggctcg
     3781 gtcagggcga agctggtgat cacctcgccg ttcgcgatcc gcggcagcca ctgcgccttc
     3841 tgggcctcgc tcccggccat caccaggccc tggctgccga tgccgacatt ggtgccgaag
     3901 gtggagcgga aggccggcgt cgtcccgccg agctcgagcg cgaccctcac ctcctcgctc
     3961 atggtgaggc cgagcccgcc atattcctcc gggatcgaga ggccgaacag ccccatctcg
     4021 cgcatctcgg cgacgatcgc ggcgggcacc tcgtcctcgc ggtcgacgcg ctcctcgagc
     4081 gggcgaagcc gctcgcggac gaagcggcgg acggcggcga ggaggaggtc gaacgattcg
     4141 gggtcgaggg gcattggtcc tccctctccc tttcagggga gagggccggg gagaggggga
     4201 gtcggtagcc agccccggcc gtcggtttgc aggagggact ccccctctcc ctgaccctct
     4261 cccctgaagg ggagagggga ctttctaata gcccttcatc cgcgccaccc tctcggcgtg
     4321 gtagttcgga tcgccgaaca gctcgttcag cgcccggtcg cgcttcatgt agaggccgat
     4381 atcatattcg tcggtcatgc cgatgccgcc gtgcatctgc acgccctcct ggacggcgag
     4441 ggtcgaggct cggcccgcct tggccttggc gaccgacacc atcgtctcgg cctccgggct
     4501 gccctcgtcg agcagctgct gcgccttgat cgccgccgag cgcgcgatct cgagctccga
     4561 atagaggtgg gcggcgcggt gctggagcgc ctggaactcg ccgatcagcc ggccgaactg
     4621 cttcctctgc ttgagatagt cgaccgtcat cgccatcgcc gccgagccga gcccgaccat
     4681 ctccgacgcc gccccggcgc ggccggcggc gagcacccgc gccagcaccg cctcgccgcc
     4741 gccgacctcg ccgatcaccg agtcggcgtc gacctcgacg ccctggaagt cgagacgggc
     4801 gccgatgctc gaatcgacca gcctgacgcc ctcgacctcg agccccttcg aatccttctc
     4861 gaccgcgaac agggtcagcc cctcgtcggt gcgggcggcg acgacggtga cgtcggccga
     4921 ggcgccctgg acgacgaact gcttgcgccc ggtcagccgg aagccgttgc cggcccgctc
     4981 ggcgcgcatc gcgatcgcct cgggccggtg cttgggccct tcgtcgacgg cgagcgccgc
     5041 caccgtctgg ccggccagga tccccgggaa ccagcgcccg ccgtggggcg tgcccttcag
     5101 agcctcgacc accgcgacgg cggtgatcag gaagggcgag ggcgtcaggt tgcggccgat
     5161 ctcctcgagc acgatccccg cctcgacatg gccgaggccg aggccgccgt ccgattcgcc
     5221 gatcaggatg ccgctgaagc cgagctcggc catctgcctc cacaaggcgt ggccgaagcc
     5281 gtcgccgcag ccgctgtcgc gatatttgcg gagctggttg gcgatgctgc cttcgtccgc
     5341 catcagctcg cgcgcggtgt cgcggagcat ggcctggtct tcggtgaggt agagcggcac
     5401 cctgtctgtc tcctacgctg agctggattg cttcgcttcg ctcgcaatga cggccttcac
     5461 ccccgtcatt gcgaggagcg aagcgacgaa gcaatccatt cccccggtgc tacgcccccg
     5521 gcaggtccaa aatcctcttg gcgatgatgc cgagctggac ctcgctggtc ccgccctcga
     5581 tcgaattggc cttggtgcga agccattcgc gggcggcctt gcccttcctg gagcggtcgc
     5641 tctcccattc gagcgagtcc gagccgcccg ccgccatcac cagctcgtgg cgggccttgt
     5701 tcagctcggt gccggcatat ttcatcatcg acggctgcgc cggatgcgcc attcccgcct
     5761 tcagctcgtc gatgaaccgc tccgacatcg cccggaacgc cagcgcgtcg acgtcgaagc
     5821 gggccatgtc ggcgcgaagc accggatcgt tcgcgacgac gtcggcgaac gcctcgccga
     5881 gactcgccgt ctggccgccg aggcccattc cgctgatcat ctcgcgctcg tggccgagca
     5941 ggtacttggc gacgtcccag cccttgttga cctcgcccac gacctggtcc ttgggcacct
     6001 tcacgtcgtc gaagaaggtc tcgcagaagg gcgaggagcc cgagatgagc aggatcggct
     6061 tggtcgacac gcccggggtc gccatgtcga acagcaggaa gctgatcccc ttgtgcttgg
     6121 gcgcctggaa atcggtgcgg accaggcaga agatccagtc cgccttgtcc gcatagctgg
     6181 tccagacctt ctggccgttg accagccaat ggtcccccat gtcctcgcac cgggtctgca
     6241 gcgaagcgag gtcggagccg gcgttcggct ccgaatagcc ctggcaccag cggatctcgc
     6301 cgcgcgcgct ctcgggcagg aagcgcttct tctgcgcctc cgtgccgtat ttcagcagcg
     6361 ccggcccgag catcgagatg ccgaagctca tcagcgggtt gcgcgccccg atccgggcca
     6421 tctcctgctt gaggaccttg gtctcggccg gggacaggcc gccgccgcca tagtccctgg
     6481 gccagtcggg gaccgtccag ccgcgctcgg ccatgcgctc catccacagc ttctgcgcgt
     6541 cgttcttgaa caccgggttg cgcccgcccc agcaggcgtc gctctcgtcg cgcatcggct
     6601 cgcgcatctc gggcgggcaa ttggcctcca gccaggcgcg cacctcttcc ctgaacgccg
     6661 ccagttcggc tatttcgctc atcgggctct ccttcgcggt tcgggatggc gcggcgggcc
     6721 ggggccgtca actgggggat atatgcgcac agccttgctt gccgccgccg ccgcgatcgg
     6781 gccggcgacc ggcgcttccg ccgccgagcg gcccgccgtc gaatattatg cccccgacga
     6841 ggccgcgcag gcggcggccc gcgccggcgg cttccgattg cccttctcgt ccgcggtccg
     6901 ggtcggcgac gtcctctatc tctcgggcgt gatcggcacc cgcgacgacg gcgccctccc
     6961 gcccgggatc gaggcccagg cgcgccagac gatggacaat atcggagcga tgctgaagcg
     7021 cgccggccgc tcgttcgacg acctgttcaa atgcaccgtc atgatcgacg acatgaagca
     7081 atggcaggcc ttcaaccagg tctacacgac ctatttcaag ccggaccgcc tgcccgcccg
     7141 aagcgccctg ggcgccgacg gcctcgcgct cggagcgctg gtcgaggtcg aatgcctggc
     7201 gcacgcgccg gccaggtgaa cctcccgcag gccaagcgcg gcttcgacgg agataggaat
     7261 aaaattagaa catagtggga acatggggcg ataataggcg ttggtccgtc ttatcgccaa
     7321 gaggagtcct atatgttagt gcgccgtatc aagcgtgctt ccgccctgct gctcgccggt
     7381 cccgccgccg tcgcctcggc gcaggcgccg caggccgcgc cgccggcgct gaaagcgccg
     7441 ccgcctccgt ccgaagagcg ggtgagcaag gcggatatcc ccgccggcca gcgctatctg
     7501 atggaccgcc acaagatctc cgcggccgaa gcggccgagc gcatcgggct cgaggaggag
     7561 gtttcgcgcc tggccaacca gctgaggacg agcgatcccg acgacttcgg ggacctgtgg
     7621 atcgagcatg agccggtcta caggatcgtc gtcgcgttcc tcaacaacga ggaccgcacc
     7681 cagcttcgcg aatcgatcgc tccgaacctg cgccgctacg ttcagttcaa gcaggtcaaa
     7741 acctcatgga aacagcgcga ggcgctcgtc gagcggatcg tcgccgcgct gaaacccttg
     7801 gcgatcgaat attattccca ttacgaccac cggaccgaca gcgtcgtcat ccagacgcaa
     7861 accgatcgcg ccgtccagca gatcaggcaa gccatcccga acgacctgca aagtttcgtc
     7921 acgatccaaa gaggccgcgt tccccaaaag gcccaggcga ccggcgcccg tgcgggagac
     7981 tatctccacg gcggctggtg gtactatctg tcgaatgccg ggggcaactg gcatgagtgc
     8041 tcatttgcat tcaacgccaa ggacaatcag aaccggccgg taatactgag tgccgggcat
     8101 tgcagccatc ccacgcaagt ctattacagc gatcactggg tatccctggc aacgccgttc
     8161 caggatcacg aggaggggaa atacgattat caggcccagc tcat
//
LOCUS       DASVQZ010000113         7912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_409811, whole genome shotgun sequence.
ACCESSION   DASVQZ010000113 DASVQZ010000000
VERSION     DASVQZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409811"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VF547_03125"
     CDS             <1..1229
                     /locus_tag="VF547_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX8261847.1"
                     /translation="RYDISRDSRLRSFVDLERVTPNSYVSIAGWAFQGLRVTDVAGQQ
                     PIALPAIDARWRLPGKVWGGRVELQANSLAILRTDGQDTQRAFAGARWDRRSLTRWGQ
                     ELVLTAYARGDVYHSSDNLLTATAIYRGRPGWHARAIGALAAEARWPLVGPFLGGTQR
                     LTPRLQLVASGATDNLELPNEDARAVDLEDSNLFALNRFPGYDRWENGPRATYGLDWG
                     LDLPGIAVRTTIGQSYRLDDKPSILPSGTGLSDRFSDYVGRTNVKFGRKLNLVHRFRI
                     DKDNLAVRRNEVDAVVGGRQTYATIGYLRLDRNIDPAIEDLRDREEIRLGGRVRFARY
                     WSIFGSTVIDLTDRREDPLSIADGYDPVRHRIGISYDDDCLEIGVTWRRDYDAAGDFR
                     RGNTFQFRVALKNLGR"
     gene            1341..2696
                     /locus_tag="VF547_03130"
     CDS             1341..2696
                     /locus_tag="VF547_03130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8261848.1"
                     /translation="MMKLGTFHRAALMLAGSLAAAAAVAQAVGDPGPTTAPSASLNIP
                     ANPQFLTEDDPNVRKATAIVNGTVITRTEVDHRLAIIRLASGGEIPAQELPRLRQQVL
                     RNLVDEVLQIQAAEQADIKVQAREVDQYFARYAQSQFGKSPEEFAAFLRASGSSPETL
                     KRQIHGEMAWSRLQRSRIEPFVNVSEDEVQAVIDRLNAAKGTQEFKVSEIFLSATPET
                     AAEARSNAQRIVQQLRGGASFLAYARQFSEASTAALGGDLGWVRAAQLPEALAAAVQT
                     MPVGAISDPIAIPGGFSIVAVTDTRQVLTADPRDAVLSLKQMTIPLPPGGTRAQLEEK
                     AQTLVRTAQSMGGCGGAEAAAAPIGAEVITNDQVRVRDLPPALQQTLLGLGLGQATPP
                     FGSSSDRVSILVLCGRDDPDPAGNLSFEQVYNQLAETRVNLRARRFLRDLRRDAVVDY
                     R"
     gene            2706..3695
                     /gene="pdxA"
                     /locus_tag="VF547_03135"
     CDS             2706..3695
                     /gene="pdxA"
                     /locus_tag="VF547_03135"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX8261849.1"
                     /translation="MIGPLAVALGDPAGIGPEITAKAWERRDSEGLPPFFAVGAPEAL
                     RAVWNGPLQVVSGPDEAALCFPDSLPVIRIDAAGETAPGQPTLEGARNSLDALELAVG
                     LARSGASSGLVTGPVSKAQLYAIGFTHPGQTEFVAERCGIAAEMVAMMLAGPTLRTVP
                     VTTHVPLRDVPGLLTTELIVTRGRTTIRALRRLFGIAWPRLAVAGLNPHAGEGGALGR
                     EEIDIVMPAIERLREEDGEVTGPHAPDTMFHARMRSTYDAALCLYHDQALVPLKTLHF
                     DEGVNITLGLPIVRTSPDHGTAFALAGQDRALPTAMIAALRMAAACAGRLAEA"
     gene            3692..4513
                     /gene="rsmA"
                     /locus_tag="VF547_03140"
     CDS             3692..4513
                     /gene="rsmA"
                     /locus_tag="VF547_03140"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX8261850.1"
                     /translation="MTPLPPLRDVIARHGLRAAKALGQNFLLDEQLLDRIARVPGDLA
                     GQRVYEVGPGPGGLTRALLRSGAEVVAVERDRRCLPALVEVADAAEGRLRVIEGDALA
                     IDEAAEAGRGAHVAANLPYNVGTALLVRWLGGEAWPPWWASLTLMFQLEVAERIVARP
                     GTGAYGRLAVLCQWRSEARIAMKVHRSAFVPPPKVMSAVVHIVPKDAPAGVRAAILEK
                     LTAAAFGQRRKMLRQSLKGLEGALEALAAVGIDPERRAETLTVEEFAAAARAMDA"
     gene            complement(4651..5154)
                     /locus_tag="VF547_03145"
     CDS             complement(4651..5154)
                     /locus_tag="VF547_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8261851.1"
                     /translation="MRLTPIALTVAIALATMASAGQGQKPDDQIDARSVALAGQAQAL
                     ASQARYQEATDLYESALAVDPRNRQAYIGLARAAQGLKLPGKAIKFYFEALKLEPNDV
                     AALAGQGEALVQRGAVERAKQNLERIKTLCAAPCPQAAQLAAVIAKGPPPEVLTAQRP
                     EVGAPKP"
     gene            complement(5185..6372)
                     /locus_tag="VF547_03150"
     CDS             complement(5185..6372)
                     /locus_tag="VF547_03150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX8261852.1"
                     /translation="MTPASRMQAAIELLDEIVAAARGGGAAADTLIARYFKTRRYAGS
                     GDRRAVRDLVYRAVRAWPEPPPSGRAAMLGLAAGDPALRAAFDGSAHGPAEAAPGEIG
                     RTPPSSAVPKWLVGRFDPAVSPADLRSLLGRAPLDLRVNRLRAARDELLPAFPGAVPT
                     PLSPIGLRLPEAVPVEESEPWRAGLVEVQDEGSQLLALACGAEPGETVLDLCAGAGGK
                     TLALAAEMRNSGRIVASDTDRGRLSRMDLRLRRAGVTIVEPRLLDPGRERHALADLVD
                     APGLILVDAPCSGTGTWRRNPELRWRLDPKRLAQYVKLQAYLLDLAAEIIRPGGRIVY
                     SVCSLLTDEGRAQAETFQGRSAMVEEPGAMAGGRAAGPGRILTPAQDGTDGFFIARWR
                     APC"
     gene            complement(6376..7857)
                     /gene="guaB"
                     /locus_tag="VF547_03155"
     CDS             complement(6376..7857)
                     /gene="guaB"
                     /locus_tag="VF547_03155"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX8261853.1"
                     /translation="MADQTRFPDIRLGLTFDDVLLQPGESSVLPSQADTSTRVTKEIG
                     LNIPILSSAMDTVTEADMAIVMAQLGGMGVLHRNFSVEEQAAAVRAVKRFESGMVVNP
                     VTVRPGQTLGDARELMARHRISGIPVTEEDGRLVGILTNRDVRFAENPRQPVSELMTR
                     DNLATVGPGVTQEEARRLLHQRRIEKLIVVDGQGRCTGLITVKDIEKAVTYPNATKDS
                     AGRLRVAAATTVGDKGFERSEALIDAECDLIVIDTAHGHNREVAAAVERIKKLSNAVQ
                     IVAGNVATAEATRALIGSGADAVKVGIGPGSICTTRVVAGVGVPQLTAIMDAAEAAAA
                     TGTPVIADGGLRTSGDLAKALAAGASTVMAGSLLAGTEEAPGETFLYQGRSYKSYRGM
                     GSVGAMARGSADRYFQHDIKDQLKLVPEGIEGQVPYKGHARDVIHQLVGGIKAAMGYT
                     GAATLEELRARARFVRITNAGLRESHVHDVAITREAPNYPTSG"
BASE COUNT         1094 a         2894 c         2789 g         1135 t
ORIGIN      
        1 gccgctacga catctcgcgc gacagccgcc tgcgctcgtt cgtcgacctc gagcgggtga
       61 cgccgaacag ctacgtctcg atcgccggct gggccttcca ggggcttcgg gtcaccgacg
      121 tcgccggcca gcagccgatc gcccttccgg cgatcgatgc ccgctggcgg ctgcccggca
      181 aggtctgggg cggccgggtc gagctccagg cgaacagcct cgccatcctc aggaccgacg
      241 gccaggacac ccagcgcgcc ttcgccggcg cgcgctggga ccggcgcagc ctgacccgct
      301 gggggcagga gctggtcctg accgcctatg cgcgcggcga cgtctaccac agcagcgaca
      361 atctgctgac cgccaccgcc atctaccggg gccggccggg atggcacgcc cgcgccatcg
      421 gagcgctcgc ggcggaggcc cgctggcctc tggtgggtcc gttcctcggc ggcacccagc
      481 ggctcactcc ccgcctccag ctcgtcgcct ccggcgccac cgacaatctc gagcttccca
      541 acgaggacgc gcgcgcggtc gacctcgagg attccaacct gttcgcgctg aaccgcttcc
      601 cgggctacga ccgctgggag aacggacccc gggccaccta cgggctcgac tggggtctcg
      661 atcttccggg gatcgcggtc cgcaccacga tcggccagag ctaccggctc gacgacaagc
      721 ccagcatcct cccctcgggg accggtcttt cggaccgttt ctccgattat gtcggccgga
      781 ccaacgtcaa gttcgggcgc aagctcaacc tcgtccaccg cttccggatc gacaaggaca
      841 atctcgctgt gaggcgcaac gaggtcgacg ccgtcgtcgg cggccggcag acctatgcga
      901 cgatcggcta cctccgcctc gaccgcaaca tcgacccggc gatcgaggac ctgcgcgacc
      961 gcgaggagat ccggctgggc ggccgggtcc gcttcgcgcg ctactggtcg atcttcggct
     1021 cgaccgtcat cgacctcacc gaccgccgcg aggatccgct gtcgatcgcc gacggctacg
     1081 acccggtgcg ccaccggatc ggcatcagct atgacgacga ctgcctcgag atcggggtca
     1141 cctggcggcg cgactatgac gcggcgggcg acttccggcg gggcaacacc ttccagttcc
     1201 gggtggcgct caagaatctc gggcgctaaa ggcctttggg gggttgcggc cgtaaaaccg
     1261 tatccacatt cgcctgaagc ccgctaagct ggggttcagc aactttgatg caccgctacc
     1321 ggcctgcatt tactggagga atgatgaagc tcggcacttt tcacagggcg gcgctgatgc
     1381 tggcgggctc gctggcggcc gcggccgccg tcgcccaggc cgtcggcgac cccggcccca
     1441 ccacggcccc gtcggcgagc ctcaacatcc ccgccaaccc ccagttcctc accgaggacg
     1501 atcccaacgt ccgcaaggcg acggcgatcg tcaacggcac cgtcatcacc cgcaccgagg
     1561 tcgaccaccg cctggcgatc atcaggctgg ccagcggcgg cgagattcca gcgcaggagc
     1621 tgccgcgcct tcgccagcag gtcctccgca acctcgtcga cgaggtcctc cagatccagg
     1681 ccgccgagca ggccgacatc aaggtgcagg cgcgcgaggt cgaccagtat ttcgcccgct
     1741 acgcccagtc gcagttcggc aagtccccgg aggagttcgc ggccttcctg cgcgcctccg
     1801 gctcgtcgcc ggagactctg aagcgccaga tccacggcga gatggcctgg tcgcgcctcc
     1861 agcgcagccg gatcgagccg ttcgtcaacg tcagcgagga cgaggtgcag gcggtcatcg
     1921 accggctcaa cgcggccaag gggacgcagg aattcaaggt ctccgaaatc ttcctctcgg
     1981 ccacccccga gacggcggcg gaagcccgga gcaacgccca gcggatcgtc cagcagctca
     2041 ggggcggggc ctcgttcctg gcctatgcgc gccagttctc ggaagcctcg accgcggcgc
     2101 tcggcggcga cctcggctgg gtccgcgccg cgcagctgcc cgaggccctc gcggcggcgg
     2161 tccagacgat gccggtcgga gcgatcagcg atcccatcgc cataccgggg ggcttctcga
     2221 tcgtcgccgt caccgacacc cgccaggtgc tgaccgccga tccgcgcgac gccgtcctca
     2281 gcctcaagca gatgaccatc cccctcccgc cgggcgggac ccgggcccag ctcgaggaga
     2341 aggcgcagac cctcgtgcgc accgcccagt cgatgggcgg atgcggcggg gccgaggctg
     2401 ccgccgctcc gatcggcgcc gaggtcatca ccaacgacca ggtgcgggtg cgcgacctgc
     2461 ccccggccct gcagcagacc ctgctcggcc tgggcctcgg ccaggcgacg ccgcccttcg
     2521 gctcgtcgag cgaccgggtc agcatcctgg tgctgtgcgg ccgcgacgat cccgatccgg
     2581 ccggcaatct gtcgttcgag caggtctaca accagcttgc cgagacccgc gtcaacctgc
     2641 gcgcccgccg cttcctgcgc gacctcaggc gcgacgcggt ggtcgactat cgctagcgcg
     2701 ccccggtgat cgggccgctg gccgtcgcgc tcggcgaccc cgccggaatc ggcccggaga
     2761 tcacggccaa ggcctgggag cggcgcgact cggaaggcct gccgcccttc ttcgcggtcg
     2821 gcgcgcccga ggccctgcgc gcggtctgga acggaccgct tcaggtcgtc tcgggcccgg
     2881 acgaggcggc gctgtgcttc ccggacagcc tgccggtgat ccggatcgac gccgccggcg
     2941 agaccgctcc cggccagccc accctcgaag gcgcgcgcaa ttcgctcgac gcgctcgagc
     3001 tcgccgtcgg cctggcccgg tccggcgcca gctcggggct cgtcaccggc cccgtctcga
     3061 aggcgcagct ctacgcgatc ggcttcaccc acccgggtca gaccgaattc gtcgcggaac
     3121 gctgcggcat cgccgccgag atggtggcga tgatgctcgc cggaccgacg ctgcggacgg
     3181 ttcccgtgac cacgcatgta ccgctgcgcg acgtcccggg cctgctcacc accgagctca
     3241 tcgtcactcg gggcaggacc acgatccgcg ccttgaggcg gctgttcggg atcgcctggc
     3301 cgcgcctggc cgtggccggc ctcaacccgc atgccggcga gggcggcgcg ctcggccgcg
     3361 aggagatcga catcgtcatg ccggcgatcg agcggctgcg cgaggaggac ggcgaggtga
     3421 ccggcccgca cgcgcccgac accatgttcc acgcccgaat gcgctcgacc tatgacgcgg
     3481 cgctctgcct ctaccacgac caggccctgg tgccgctgaa gacgcttcac ttcgacgagg
     3541 gcgtgaacat caccctcggg ctgccgatcg tccgtacctc gcccgaccac ggcaccgcct
     3601 tcgcgctcgc gggccaggac cgggcgctgc cgacggcgat gatcgccgcg ctcaggatgg
     3661 cggccgcctg cgccgggcgg ctggcggaag cgtgactccc ctgcccccgc tgcgcgacgt
     3721 gatcgcgcgg cacgggctgc gcgcggccaa ggcgctcggc cagaatttcc tcctcgacga
     3781 gcaattgctc gaccgcatcg cgcgggtccc gggcgatctg gcggggcagc gggtctacga
     3841 ggtcggcccg ggccccggcg gcctgacccg ggccctgctc cggtccgggg ccgaggtggt
     3901 cgcggtcgag cgcgaccgcc gctgccttcc cgccctggtc gaggtggcgg acgcggcgga
     3961 agggcggctt cgggtgatcg agggcgatgc gctggcgatc gacgaagccg ccgaggcggg
     4021 gcgcggcgcc catgtcgccg ccaacctccc ctacaatgtc ggaaccgctc tgctggtgcg
     4081 ctggctcggc ggcgaggcct ggcccccctg gtgggcttcg ctgaccctga tgttccagct
     4141 cgaggtggcc gagcgcatcg tcgcccggcc cggcaccggc gcctatggcc gcctcgcggt
     4201 gctctgccag tggcgcagcg aggcccggat cgcgatgaag gtccaccgct ccgccttcgt
     4261 gccgccgccc aaggtgatgt cggcggtggt ccacatcgtc ccgaaggacg cgcctgcagg
     4321 cgttcgggcg gcgatcctcg agaagctcac cgccgcggcc ttcggccaga gacgcaagat
     4381 gctgcgccag agcctgaagg ggctggaggg cgccctggag gcgcttgcgg cggttgggat
     4441 cgacccggaa cggcgcgccg agacgctgac ggtggaggaa ttcgccgccg cggcgagggc
     4501 gatggacgcc taatcctccc catgcgcagc atggggaggg ggaccatgcg aagcatggcg
     4561 gaggggtgga ttgccgacac cttcgccagc tacccctcca ccgcccttcg ggtggtcccc
     4621 ctccccaccg ctatgcggcg gggaggattt tcaaggcttc ggcgctccga cctcgggccg
     4681 ctgggcggtg agcacctcgg gcggcgggcc cttggcgatc acggcggcga gctgggccgc
     4741 ctgcgggcag ggcgcggcgc acagcgtctt gatccgctcc agattctgct tggcccgctc
     4801 gaccgcgccg cgctgcacca gggcctcgcc ctggccggcg agggcggcga cgtcattggg
     4861 ctcgagcttc agcgcctcga agtagaattt gatcgccttt cccggaagct tcagcccctg
     4921 cgcggcgcgg gcgaggccga tataggcctg gcggttgcgg ggatcgacgg cgagcgcgga
     4981 ttcgtagagg tcggtcgcct cctggtagcg cgcctgcgag gcgagcgcct gggcctggcc
     5041 ggcgagcgcc accgagcgcg cgtcgatctg gtcgtcgggc ttctggccct ggccggcgct
     5101 tgccatggtc gccagagcga tggcgacggt gagcgcaatg ggggtgaggc gcatcagtaa
     5161 ctccatgatc ctgtcgcggg cggcttagca tggcgcccgc caccgcgcga tgaaaaaccc
     5221 gtccgtgccg tcctgcgccg gggtgaggat ccgccccggg ccggcggccc gtccacccgc
     5281 cattgcaccc ggttcctcaa ccatggctga acggccctgg aaggtctcgg cctgggccct
     5341 cccctcgtcg gtgagcagcg agcagacgga gtagacgata cgcccaccgg gccggataat
     5401 ttccgctgcg agatcaagca gataggcctg gagcttgaca tattgcgcga gccgcttcgg
     5461 atcgagccgc cagcgcagct ccgggttgcg ccgccaggtc ccggtgccgg agcagggcgc
     5521 gtcgaccagg atcagcccgg gcgcgtcgac gaggtcggcg agagcgtgcc gctcgcggcc
     5581 cggatcgagc aatcgcggct cgacgatggt gaccccggcc cgcctcagcc tgaggtccat
     5641 gcgcgacagc cggccgcggt ccgtgtcgga cgcgacgatg cggccggaat tgcgcatttc
     5701 ggcggcgagg gcgagggtct tgccgccggc gccggcgcac aggtcgagga ccgtctcccc
     5761 gggctcggcg ccgcaggcga gggcgagcag ctggctgcct tcgtcctgga cctcgaccag
     5821 ccccgcgcgc cagggctcgc tctcctcgac cggcacggcc tcggggagtc gcaggccgat
     5881 cggcgacagg ggcgtcggga ccgcgccggg aaaggcgggc aggagctcgt cgcgggcggc
     5941 gcgcagcctg ttcacccgca ggtccagcgg cgcccggccg agcagcgagc gcaggtccgc
     6001 gggcgacacg gccggatcga accggccgac gagccatttc gggacggcgg aggacggcgg
     6061 cgtccggccg atctcgccgg gcgccgcttc ggcaggaccg tgggccgagc cgtcgaaagc
     6121 ggcgcgcagc gcggggtcgc cggccgccag gccgagcatc gccgcgcggc ccgagggggg
     6181 cggctcgggc caggcgcgga cggctcggta gacgaggtcg cgcaccgccc gccggtcgcc
     6241 cgagccggca tagcgccggg tcttgaaata gcgggcgatc aaggtgtcgg cggcggcgcc
     6301 gccgcccctc gccgcggcga cgatctcgtc gagcagctcg atcgccgcct gcatccgcga
     6361 ggcgggggtc acgaatcagc ccgaggtcgg ataattgggc gcttcccggg tgatggcgac
     6421 gtcgtggacg tggctttcgc gaagcccggc gttggtgatc cggacgaacc tcgcccgggc
     6481 ccggagctcc tccagcgtcg ccgcgccggt atagcccatc gccgccttga tgccgccgac
     6541 cagctggtgg atcacgtcgc gggcatggcc cttgtagggc acctgcccct cgatcccctc
     6601 ggggacgagc ttcagctggt ccttgatgtc gtgctggaaa tagcggtcgg ccgagccgcg
     6661 cgccatcgcc ccgaccgagc ccatgccgcg gtaggatttg tacgagcgcc cctggtagag
     6721 gaaggtctcg ccgggcgcct cctcggtgcc ggccaggagc gagcccgcca tcaccgtcga
     6781 ggcgccggcg gcgagcgcct tggcgaggtc gccgctggtc ctcagcccgc cgtcggcgat
     6841 gaccggcgtg ccggtcgccg cggccgcctc ggcggcgtcc atgatcgcgg tcagctgcgg
     6901 caccccgact ccggcgacga cccgggtggt gcagatcgag cccggaccga tgccgacctt
     6961 gaccgcgtcc gcgcccgagc cgatcagcgc cctcgtcgcc tcggcggtgg cgacgttgcc
     7021 ggcgacgatc tggacggcgt tggacagctt cttgatccgc tcgaccgcgg cggcgacctc
     7081 gcggttgtgg ccgtgggcgg tgtcgatgac gatgaggtcg cattcggcgt cgatcagcgc
     7141 ctcggaccgc tcgaacccct tgtcgccgac ggtcgtcgcc gccgccacgc gaagccggcc
     7201 ggccgagtcc ttggtcgcgt tcggataggt caccgccttc tcgatgtcct tgaccgtgat
     7261 cagcccggtg cagcgcccct gcccgtcgac cacgatcagc ttctcgatcc gccgctggtg
     7321 gagcagcctt cgggcctcct cctgggtgac gccggggccg accgtggcga gattgtcgcg
     7381 ggtcatcagc tcggacaccg gctggcgcgg attctcggcg aagcggacgt cgcggttggt
     7441 caggattccg accaggcggc cgtcctcctc ggtcaccggg atgcccgaga ttcggtgccg
     7501 ggccatcagc tcgcgggcgt cgccgagcgt ctggccgggc cggaccgtca ccggattgac
     7561 caccatcccg ctctcgaacc tcttgaccgc ccggaccgcg gcggcctgct cctcgacgct
     7621 gaaattgcgg tggagcacgc ccatcccgcc gagctgcgcc atgacgatcg ccatgtccgc
     7681 ctcggtcacc gtgtccatcg ccgaggagag gatcgggatg ttgagcccga tctccttcgt
     7741 cacccgggtg ctggtgtcgg cctggctcgg aagcaccgac gattcgccgg gctgaagcag
     7801 cacgtcgtcg aaggtgaggc caaggcgtat gtcggggaag cgggtctgat cggccatgtg
     7861 cgcctatgtc agggaaagcg cgattcgcca agccttgcag cgagagccgg aa
//
LOCUS       DASVQZ010000114         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_24992, whole genome shotgun sequence.
ACCESSION   DASVQZ010000114 DASVQZ010000000
VERSION     DASVQZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24992"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..800)
                     /locus_tag="VF547_03160"
     CDS             complement(192..800)
                     /locus_tag="VF547_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX8261854.1"
                     /translation="MSNPEIRTICVYCGSGRGSDPVFVEAAVALGRAMAESGIGLVYG
                     GGNIGLMGELARSVLDHGGHVTGIIPEFLKQRERMLDDVQETIVVTDMHTRKRLMFER
                     ADAFVAMPGGIGTLEELVEQMTWAQLGRHTKPILLLDTKGFWQPLLTLLQHMREQGFI
                     REGLDLSYLVAHRVEEVLHMLRAAVRRSPESPGAAALIEERF"
     gene            984..>1935
                     /locus_tag="VF547_03165"
     CDS             984..>1935
                     /locus_tag="VF547_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261855.1"
                     /translation="MTWFGVLKEPRPAGPTAPEARAPAGTPPAPSDTARAPSPSPAPT
                     APPRSESAAAPLPAVASPTTAEAPRETAAAPAGPPRQAAPAPPEPVREAAAPAAAPLA
                     PSFDVVRVEPSGESVIAGRSVPGAIIDLLRNGTSHARAAADQSGLFALVPPPLPPGSH
                     EIVLQSIAPDGTRTRSRDSITVVIAEDKATKPLVALASPDKPTVLLSNPDRPSAPPEP
                     PASRGPNVAAAPAPGAPADPAARRPEPPAPAPAQRPPVRISAIDAEDGGRLFVSGQAA
                     PGATVRLYLNDTLIAPGGAGADGKVGFAISRGVRPGGYRVR"
BASE COUNT          257 a          725 c          694 g          259 t
ORIGIN      
        1 ggtcccggca ggcgcggcgg tcccggcgcc gctcggcgcc ggcgccggcg cggtctgggc
       61 gagggcgctg cccgccagga gcgctgaaac ggtcagtccg gcggcgaggg ctaggcggcg
      121 catcgatcat ctccggcaac agggtcgctc aaggccaagc taagcccatt tccgcaacgc
      181 tggcgagagg atcagaagcg ctcctcgatc agcgcggccg cgccggggct ctcggggctg
      241 cggcgcaccg ccgcgcgcag catgtgcagg acctcctcga cccggtgagc gacgaggtaa
      301 ctgagatcga gcccctcgcg gatgaagcct tgctcgcgca tgtgctggag cagcgtgagc
      361 agcggctgcc agaagccctt ggtgtcgagg aggaggatcg gcttcgtgtg gcggccgagc
      421 tgggcccagg tcatctgctc cacgagttcc tcgagggtgc cgatgccgcc cggcattgcc
      481 acgaaggcgt ctgcccgctc gaacatcagg cgcttccggg tatgcatgtc ggtgaccacg
      541 atggtctcct gcacgtcgtc gagcatgcgt tcccgctgtt tcaggaactc ggggatgatg
      601 ccggtgacgt ggccgccatg gtcgaggacg gaccgggcga gctcgcccat gaggccgatg
      661 ttgccgccgc cgtagacgag cccgatgccg ctctcggcca tcgcgcggcc gagggcaacg
      721 gcggcctcga cgaagacggg atcgctgccc cgtccggagc cgcagtacac gcaaatcgtc
      781 cggatctcag ggttggacat tccgccgtgg ggcctcccgg tcgaatcgta gagcatttct
      841 tgtccgctca gcgtagctta tgggcgggta aacggcttgc atgaggcaaa gtgccggcct
      901 tggaggtttg agggatcctg atgcgtccac ggcagggcag aacggccatc gccgttctcg
      961 gcgtcgcagc cttcgcggcg gtcgtgacct ggttcggcgt cctcaaggag ccgcgcccgg
     1021 cgggcccgac cgcgccggag gcgagggcgc ccgccggcac cccgcccgcc ccgagcgaca
     1081 ccgcgcgcgc accgtcgccg tcgccggcgc ccaccgcccc gccgcgcagc gagagcgccg
     1141 ccgcgccgct tccggcggtc gcgagcccca cgaccgcgga agctcctcgc gaaacagccg
     1201 ccgccccggc cgggccgcca cgccaggctg cccctgcgcc gcctgaacct gtgcgcgagg
     1261 ccgcggctcc ggcggcagcg ccgctggcgc cgagcttcga cgtcgtccgg gtcgaaccga
     1321 gcggcgagag cgtcatcgcg ggccggtcgg tcccgggcgc gatcatcgat ctgttgcgca
     1381 acggcacgag ccacgcccgt gccgcggccg accagtccgg cctcttcgcc ctggtgccgc
     1441 ccccgctgcc gcccggctcg cacgagatcg tcctgcaatc gatcgcaccc gacggcacgc
     1501 ggacgcgctc ccgcgacagc atcacggtcg tcatcgccga ggacaaggcg acgaagccgc
     1561 tcgtggcgct cgcctcgccc gacaagccga cggtgctgtt gtcgaatccc gaccggccct
     1621 cggccccgcc cgagccgccg gcgtcgcggg gtcccaatgt cgccgccgcg cccgcgcccg
     1681 gcgctccagc cgaccccgcc gcacggcggc cggagccccc ggcgccggcg cctgcccagc
     1741 ggcctccggt ccgtatctcc gccatcgatg cggaggacgg cggccgcctg ttcgtatcgg
     1801 gtcaggcggc gccgggcgcg accgtgcggc tctacctcaa tgacaccctg atcgcgcccg
     1861 gcggggcggg ggcggacggg aaagtgggct ttgccatcag ccggggcgtg cgcccgggcg
     1921 gctatcgcgt ccgcc
//
LOCUS       DASVQZ010000115         3740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_793334, whole genome shotgun sequence.
ACCESSION   DASVQZ010000115 DASVQZ010000000
VERSION     DASVQZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793334"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF547_03170"
     CDS             <1..323
                     /locus_tag="VF547_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261856.1"
                     /translation="GPAAFVRSGDGATALGLWRVEGPAGEPVGHYRVGFRREAKAWRI
                     TRMNLVEPPTDPDAVAYYCHAPGDSEAYAKAKAERDAKRERTRAERAAREAEAPDRAR
                     RRGG"
     gene            complement(597..962)
                     /locus_tag="VF547_03175"
     CDS             complement(597..962)
                     /locus_tag="VF547_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8261857.1"
                     /translation="MARKKRPPNWFLPGWVHKCLSRRRKRDRLREIWGDDCWWCDRPM
                     RFDGPPNCGKAATIEHLRPKAKGGTWALDNLRLCHVGCNRHLGDRSREEKERMRTAMR
                     PRPAEPRPRTPDPGGRATA"
     gene            1115..1645
                     /locus_tag="VF547_03180"
     CDS             1115..1645
                     /locus_tag="VF547_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8261858.1"
                     /translation="MRKLVLAAATAACAMPIAFSAPALAQQGYETRRETRECRRELRR
                     ADNRREYRRELRECRREIARARNRDWRTYNRYDYNRYEPGVDGYYADRYYRDGRYYGE
                     TRLSRDDRIYRGSDGRYYCRRSDGTTGLIVGGAVGALIGSSIDNGRSSILGTLIGAGA
                     GAALGREIERGGLRCR"
     gene            1703..1993
                     /locus_tag="VF547_03185"
     CDS             1703..1993
                     /locus_tag="VF547_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEX8261859.1"
                     /translation="MPQGDKSSYTGKQKRKAEHIAESYEERGVPKKEAKSRAYATVNK
                     DSGGGNKSGSGRGKKDGNSSAREGGRKGGATASSRSAAAKKGWETRRRRGKA"
     gene            2014..2250
                     /locus_tag="VF547_03190"
     CDS             2014..2250
                     /locus_tag="VF547_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261860.1"
                     /translation="MLRRLPLVAGLVAALAALVLVGPVAVAVALLRSSSDAQGPSLAA
                     QLLIAVLVLAVAALAGFAAWGLARLVLRLVGRGG"
     gene            2300..3064
                     /locus_tag="VF547_03195"
     CDS             2300..3064
                     /locus_tag="VF547_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8261861.1"
                     /translation="MDRGSSASSETIEEAQAVARRFIAQRYPGAMAALLAGSRARGEG
                     GPRSDYDIFLLFDALPQGAWRERAFFEGRTFELFAHDRGTLSYFCRTVVGRSGVPSVP
                     QMVVDGIALLGEGGTALAAARSLAARTLAAGPPPLDPPTRLSRVSAITELTEILADSP
                     PGPARIVVGGALYPMLADFALRAAGAWGASMKRLPAAVAELDPALAGRFDAAFTALFR
                     DGDPRPVLELADSILTRHGGRPRDGFRIASPPSWRD"
     gene            3122..3478
                     /locus_tag="VF547_03200"
     CDS             3122..3478
                     /locus_tag="VF547_03200"
                     /inference="COORDINATES: protein motif:HMM:NF019612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8261862.1"
                     /translation="MRILDVDDSPPAEAAALTGHLVPGEAVHAAYVAPTGAILFTDRR
                     IVIALREHLLEARVETSSWPYRSVRHFSIQEGETAESRAAVRIWLGDEPQPLHLRGNP
                     GTDLRALQRLLADRLA"
     gene            complement(3482..>3740)
                     /locus_tag="VF547_03205"
     CDS             complement(3482..>3740)
                     /locus_tag="VF547_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261863.1"
                     /translation="IPLDGGADALGSAGAHMLDVGLSSTARIAGFFGIAPEGAADGRP
                     AAARLAGRPARPAAAPALPPETAGGVQQVIEDALRKAGLMR"
BASE COUNT          475 a         1408 c         1351 g          506 t
ORIGIN      
        1 gcggtccggc cgccttcgtc cggtccggcg acggcgccac cgcgctcggg ctgtggcggg
       61 tcgaagggcc ggcgggcgag ccggtcggcc attaccgggt cggtttccgg cgcgaggcca
      121 aggcctggcg gatcacccgg atgaacctgg tcgagccgcc gaccgatccc gacgccgtcg
      181 cctattactg ccacgcgccc ggcgattcgg aagcctatgc caaggcgaag gccgagcgcg
      241 acgccaagcg cgagcgcacg cgggcggagc gggccgcccg cgaggccgag gcccccgacc
      301 gcgcccgccg ccgcggcggc tgagcggccg gccccggctc gatcgcgcgg cgccgcgctc
      361 acctgtgcgt catgcggacg gccgccgcgg ccctcccggc cacgggctgg ccctgcgtcc
      421 ccgggaccgg ctccgcccgg ccggcgggta gagcgctcgg cagtccgatt gcgccatgtc
      481 cgttcgggct gagcttgtcg aagccctctc tttttctccc atggaacgaa aaggaaagcc
      541 cttcgacagg ctcagggcga gagggtggga cggattcgac ccaatcccgc aatggtctag
      601 gcggtcgcgc ggccgccggg gtcgggggtg cggggccggg gctccgccgg gcgaggccgc
      661 atggcggtcc gcatccgctc cttctcctcc cggctgcggt cgccgaggtg gcggttgcag
      721 ccgacatggc agaggcgaag attgtcgagc gcccaggtcc cccctttcgc cttcggccgg
      781 agatgctcga tcgtcgccgc cttgccgcaa ttgggcggtc cgtcgaagcg catcggacgg
      841 tcgcaccacc agcaatcgtc gccccagatc tcgcggagcc ggtcccgctt gcggcggcgg
      901 ctgaggcact tgtgcaccca gccgggaagg aaccagttcg gaggccgttt cttgcgagcc
      961 atggcccgtg atggcggacg aagcctttcg cttcggtaaa gcgccggtct gaggctcgcg
     1021 aagccgacct gggggcggcg gcgatgaaac ccgccgcggc cgccgtccgt tgtccgtctc
     1081 gacccccgta cggggccttc cacaggagag tagcatgcgt aaactcgttc ttgccgcggc
     1141 cacggcggcc tgcgccatgc cgatcgcctt ttcggcgccg gcccttgccc agcagggcta
     1201 cgagacccgc cgcgagacgc gcgaatgccg ccgcgagctg cgccgggccg acaatcgccg
     1261 cgaatatcgc cgcgagcttc gcgaatgccg ccgcgagatc gcccgcgccc gcaaccgcga
     1321 ctggcgcacc tacaaccgct acgactacaa ccgctacgag ccgggcgtgg acggctatta
     1381 tgccgaccgc tattaccgtg acggccgcta ttacggcgag acccggctga gccgcgacga
     1441 ccgcatctac cgcggctcgg acgggcgcta ttactgccgc cgctcggacg gcaccaccgg
     1501 cctcatcgtc ggcggcgcgg tcggcgccct gatcggcagt tcgatcgaca acgggcgctc
     1561 gagcatcctg ggcaccctga tcggcgccgg cgccggagcg gcgctcgggc gcgagatcga
     1621 gcgcggcggc ctccgctgcc gctaggcgag gcgaccaacc tgatctgccg cggccccggc
     1681 cgcggcggga ggaggaaaga agatgccgca gggcgacaag agcagctaca ccggcaagca
     1741 gaagcgcaag gccgagcata tcgccgagag ctacgaggag cgcggagtcc cgaagaagga
     1801 ggccaagtcg cgcgcctacg ccaccgtcaa caaggattcg ggcggcggca acaagtccgg
     1861 ctcgggccgc ggcaagaagg acggcaattc ctccgcgcgc gagggcggcc ggaagggcgg
     1921 cgccaccgcc tcgagccgaa gcgccgccgc caagaagggc tgggagaccc gccgccggcg
     1981 cggcaaggcc tgatcccggg gaaggcgccg cctttgctcc gccgacttcc gctggtcgcc
     2041 ggcctcgtcg ccgcgctcgc cgcactggtg ctggtcggtc cggtggcggt cgccgtcgcc
     2101 ctgcttcgct cctcgtcgga cgcgcagggg ccgagcctcg ccgcgcagct gctcatcgcc
     2161 gtcctcgtcc tggccgtggc ggcgctcgcc ggcttcgccg cctggggcct cgcccggctc
     2221 gtgctgcgcc tggtcggccg gggcggctga gcccttgccg ccgccgccgg gcaatgcgac
     2281 accgggcgag ggggtggcga tggaccgcgg cagttccgca agctcggaga cgatcgagga
     2341 ggcccaggcc gtcgcccgcc gcttcatcgc gcagcgttat ccgggtgcaa tggccgccct
     2401 tctcgccggg tcgcgggccc ggggcgaggg cgggccgagg tccgattacg acatcttcct
     2461 gctgttcgac gccctgccgc agggcgcgtg gcgcgaacgg gccttcttcg agggcaggac
     2521 cttcgagctg ttcgcccacg atcgcggcac cctctcctat ttctgcagga ccgtggtcgg
     2581 ccggtcgggc gtcccctcgg tgccgcagat ggtcgtggac gggatagccc tgctcggcga
     2641 gggcgggacc gcgctcgccg cggcgcgctc gctggccgcg aggacgctcg ccgccgggcc
     2701 tccgccgctc gatccgccga ctcggctgag ccgggtctcc gctatcaccg agttgaccga
     2761 gatcctggcc gattccccgc cgggccccgc ccgcatcgtg gtcggcggcg cgctctatcc
     2821 gatgctcgcc gatttcgcgc tgcgcgcggc gggggcgtgg ggcgcctcga tgaaacggct
     2881 tccggcggcc gtggcggagc tggatccggc cctggccggc cgcttcgacg ccgcgttcac
     2941 cgccctgttc cgcgacggcg acccgcggcc ggtgctcgag ctggcggatt cgatcctgac
     3001 ccgccacgga ggccggcccc gcgacggctt ccgaatcgcc tcgccgccgt cctggcggga
     3061 ctgatcggcg cgcggtccgg cgccgattgc gtcggcggcg ccggcgtccc atctacggcc
     3121 gatgcgaatc ctcgacgtcg acgacagccc gcccgccgaa gccgccgcgc tgaccggcca
     3181 cctcgtcccc ggcgaggcgg tccatgccgc ctatgtcgct ccgaccgggg cgatcctgtt
     3241 caccgaccgc cgaatcgtca tcgccctgcg cgagcatctg ctcgaggccc gggtcgagac
     3301 cagctcttgg ccctaccgct cggtccgtca tttctcgatc caggagggcg agactgccga
     3361 aagccgcgcc gcggttcgga tctggctcgg cgacgagccg cagccgctcc acctgcgggg
     3421 caatccgggc accgacctgc gcgccctcca gcggctgctc gctgaccggc tcgcctgagg
     3481 gtcagcgcat caggcccgcc ttgcgcaggg cgtcctcgat cacctgctgg acgcccccgg
     3541 ccgtctccgg cggcagggcc ggcgccgcgg ccggacgcgc ggggcgtccg gcaaggcgtg
     3601 ccgcggccgg ccggccgtcc gccgcgccct ccggggcaat gccgaagaag cccgcgatcc
     3661 gggccgtcga gctcagcccg acgtcgagca tgtgcgcgcc cgccgagccg agcgcgtcgg
     3721 cgccgccgtc gagcgggatg
//
LOCUS       DASVQZ010000116         6843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_503769, whole genome shotgun sequence.
ACCESSION   DASVQZ010000116 DASVQZ010000000
VERSION     DASVQZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503769"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VF547_03210"
     CDS             complement(<1..1616)
                     /locus_tag="VF547_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261864.1"
                     /translation="MGVDPKDLGPCGAEDLDPKRPDNRPRLPEIAYRIGTQPEFLKRM
                     KWRIPRQTVAVAGGGTLNPLAALRTRESSDPTLALMDAFACSLDVLSFYSERIANEGY
                     LGTAVQRRSMVELARSIGYQLAPGVAASVQLSFTVEEADDPFRAVEIPAGVQAMSVPQ
                     AKGELPQVFETVEPILARAEWNAMPVRTEHSQNLALYWNAGDSEDERNGNLYLFDLDN
                     SFDADETTDSELETFADTAALEPYFPLDPSLDLEGALVKRIEDFQYNPAIEPVLKAVP
                     VDETFLRGVGLGMKPGQRIVVIGVRIDEDGGRHVACALHRVVAASEDRAFDMTVLESL
                     PHGAAPRPVRRTPRLRLPRLKLPRMPTQRIAFNTDSVTSVVRESAWSGDSLSALVRTQ
                     AWPRTKLMQILRMPRVVLAPETSEATPGFYLLRDDTGFFGNTAPMQEQLAVPAGATAN
                     PDDSLFHPYSQPWDKDGGRTVWVDSQGASLLTDAEGKLRPGGVHVFLDREMKELVPNG
                     WAALETPDSQVQVFRIGTAAAQSRADYGMTG"
     gene            complement(1616..4147)
                     /locus_tag="VF547_03215"
     CDS             complement(1616..4147)
                     /locus_tag="VF547_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX8261865.1"
                     /translation="MSGGAIQHCGTGRRRQRVADATIQTEGGELLNGIDYLEVIDRDA
                     PSEALRQRLLDITFIRPDGVVAGAAALLDPDNFRIEGGSRVRNVRVTQVAKGAGDRTL
                     RLTLDRYGDFSTYRLAVQVGADNPNPPPFIDRMLAAVDFHFKVECPSDFDCEAPDPEV
                     PRRDFGPPLNYLARDYASFGQLMLDRMATTLPDWTERNAADVGITLVEALAHAADQTS
                     WFQDAVHTEAFLGRARLRQSVLRHARLLGYAASEGCNARVAVAIEAANDDSGTPLLPA
                     GTRLLSRPLQISGSVPAVLPPDPEQFEALVSAGSIVFETLDPVDSIRVARNEIELHDW
                     GDDACCLLPGSTAAWLVGTKAALGLARGDLLILEERIPFGRSALDPPDPSHRQVVRLA
                     ADPVEAKDEVMGVTVVRVDWHEEDALRFPLTLREEAGVPAAVAIGNVVLADEGRTIDY
                     GLAPNQAGADAIAVGGPDDTGLLPDDGAGTRLRYRLDAGSVVYAPAWESEEAVERSAA
                     AALAPDPAEAVAQVRLAGDGESWAAVPDLLATDRFTPAVKVEPAEGGGAYVLFGDGAA
                     GRLPSEGAVFQARIRVGGGRRGNVAADGIGHVVAAGAPLIAGIRNPVAASGGLDAEPT
                     TAIRIAAPFAFRRQRRAVTPGDYAAAAMEHDDVQRAQAKRRWTGSWHTVFLAVDRLGG
                     REVDAAFEESLRRHLASRRLAGHDLEIVPPRFVPLDVAFYICVCADHYPADVERDLLD
                     AFSSGYSRNGQPGFFHPDNFSFGDNVHLSRLIARGMAVEGVLWMGVRDAAGQAVGRFG
                     RLDQPDVDYEADGEIPIAADEVARLDNDPNYPDFGRLRLIMAGGR"
     gene            complement(4144..4518)
                     /locus_tag="VF547_03220"
     CDS             complement(4144..4518)
                     /locus_tag="VF547_03220"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX8261866.1"
                     /translation="MTARGPVHFAFPYRVAADGATARADSEEHVRDLIEAILFTRRGE
                     RVNRPDFGAGVAELIFAENAPEMAAAAQHMVQSALQQWLSGVIEIRGVDTFSRDSLLS
                     ITVRYRALDEGVERSVNVTRAV"
     gene            complement(4515..4847)
                     /locus_tag="VF547_03225"
     CDS             complement(4515..4847)
                     /locus_tag="VF547_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261867.1"
                     /translation="MSLAVVTTATMIQCPHAIPATLVSSASKVLVDSMPPLVMGDKPT
                     VAGCPFMVGSKPQPCATVLLTKTATKVLAENKAVLLMNPADIAQSADQIPQGPAVWGN
                     IQSKVLAT"
     gene            complement(4844..5341)
                     /locus_tag="VF547_03230"
     CDS             complement(4844..5341)
                     /locus_tag="VF547_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX8261868.1"
                     /translation="MSCFGKFRGEVQNNLDPLGLGRIQVSVPGLLDNSTLAWAMPCLP
                     GAGPDVGIFVIPPVGAKVWVEFERGDLDYPIWSGGFWGTGEAPAPMGPTQAMTRVWAG
                     DNFRIEVVDMPGAGTLEITLTTPGGEAKLAADATALELRWGKSSVKLSVDGVSINGSN
                     LKVLP"
     gene            complement(5338..6495)
                     /locus_tag="VF547_03235"
     CDS             complement(5338..6495)
                     /locus_tag="VF547_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261869.1"
                     /translation="MSLLGNLLGIKANILIGPSPVALPMPAEALQALEELDIRLSDRE
                     PSGFRMVLRAGRSGPADFLETPLVADPRFNRGSRLVVSILFDAVPTVIFDGLVSERHV
                     SPGEGSSQGRIVLLGHDLSFEMDREVKNVERPAMDEMTIANLIALSYAQFGIIPMVMP
                     PKVIDPPLPVERTPQQRCSDWDYLNAMARRHGYETYVEAGPVPLTNILYWGPPVRPGA
                     PQRTLTVSSGPVTDAYDIRAANSAEELTTVETQVQDRQTGATMPVKAVTPSQTPLGAL
                     PEAAARMGKTRTTPIETSGLTFAQAMARAQGAVDESARRVVRVTGTVDSTRYNGALKA
                     RDQIYLRGAGAMFDGLYKVAEVRHRIRPGEYEQGFVLTRGELGAASLAVRS"
     gene            complement(6492..6797)
                     /locus_tag="VF547_03240"
     CDS             complement(6492..6797)
                     /locus_tag="VF547_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261870.1"
                     /translation="MIAADSRYASAPQLVYVTSSGQPVTYLAPRILPQPDGFQPVLRH
                     RVTDSDRIDLLAWRNLGAPTAWWQIADANRAMEPSALPGAPGDVVLVPVAGTVSLVG"
BASE COUNT          974 a         2306 c         2567 g          996 t
ORIGIN      
        1 ccggtcatcc cgtaatcggc gcgggactgg gcggcggcgg tgccgatccg gaacacctgg
       61 acctgactgt ccggcgtctc cagggccgcc cagccgttgg gcaccagctc cttcatctcc
      121 cgatcgagga agacgtggac tcccccgggg cgcagctttc cctcggcgtc ggtcagcagc
      181 gacgcgccct ggctgtccac ccagaccgtt cgaccgccgt ccttgtccca gggctgcgaa
      241 taggggtgga acagggaatc gtctgggttg gcggtggcgc ccgccgggac ggcgagctgc
      301 tcctgcatgg gcgcggtgtt gccgaagaag ccggtgtcgt cgcgcagcag gtagaagccg
      361 ggcgtggcct ccgaggtctc gggcgcgagg acgacccgcg gcatccgcag gatctgcatc
      421 agcttcgtcc gcggccaggc ctgggtccgg accagggcgc tgagggaatc gccgctccac
      481 gccgattcgc gcaccaccga ggtcaccgag tccgtattga aggcgatgcg ctgggtcggc
      541 atccgcggca gcttcagccg cggcagccgg aggcgcgggg tccggcggac cgggcgcggc
      601 gcggcgccgt gcggaagcga ttcgagcacc gtcatgtcga aggcgcggtc ctcgctggcg
      661 gcgacgacgc ggtggagcgc gcaggcgacg tggcggccgc cgtcctcgtc gatgcggacg
      721 ccgatgacga cgatccgctg gcccggcttc atcccgaggc cgacgccgcg caggaaggtt
      781 tcgtcgaccg ggaccgcctt gagcaccggc tcgatggccg gattgtattg gaaatcctcg
      841 atccgcttga cgagggcgcc ctcgaggtcg agcgacgggt cgagcgggaa gtagggctcc
      901 agagcggccg tgtcggcaaa ggtctcgagc tccgagtcgg tcgtctcgtc ggcgtcgaag
      961 ctgttgtcga ggtcgaacag gtagaggttg ccgttcctct cgtcctcgga gtcgccggcg
     1021 ttccagtaca aggcgaggtt ctgggaatgc tcggtccgca ccggcatcgc gttccattcc
     1081 gcccgcgcaa ggatcggctc gaccgtctcg aagacctggg gaagctcgcc cttcgcctga
     1141 ggcaccgaca tggcctggac gccggccggg atctccaccg cccggaacgg gtcgtcggct
     1201 tcctcgaccg tgaagctgag ctgcaccgag gcggcgaccc cgggcgcgag ctgatagccg
     1261 atcgagcggg cgagctcgac catcgagcgc cgctgcaccg ccgtgccgag atagccttcg
     1321 ttggcgatcc gctccgaata gaagctgagg acgtcgaggc tgcaggcgaa ggcgtccatc
     1381 agcgcgagag ttgggtcgga gctctcgcgg gtgcgcagcg cggcgagcgg attgagggtg
     1441 ccgccgccgg cgaccgcgac cgtctggcgc gggatccgcc atttcatccg cttgaggaat
     1501 tcgggctggg tgccgatccg gtaggcgatc tccggcagcc gcggacgatt gtcgggacgc
     1561 ttcgggtcga gatcctcggc gccgcacggt ccgaggtcct tggggtcgac gcccatcagc
     1621 ggcctcccgc catgatcagg cgcagccgcc cgaaatccgg ataattggga tcgttgtcga
     1681 ggcgcgcgac ctcgtccgcg gcgatcggaa tctcgccgtc ggcctcatag tcgacgtccg
     1741 gctggtcgag ccggccgaag cgcccgacgg cctggccggc ggcgtcgcga acgcccatcc
     1801 acagcacgcc ttcgaccgcc atcccgcggg cgatcagccg cgacaggtgg acgttgtcgc
     1861 cgaagctgaa attgtccgga tggaagaagc ccggctggcc gttgcgcgaa tagccgctgg
     1921 agaaggcgtc gagcaggtcg cgctcgacgt cggccggata atgatcggcg cagacgcaga
     1981 tgtagaaggc gacgtcgagc gggacgaacc ggggcgggac gatctcgaga tcgtggccgg
     2041 cgagccggcg ggaggcgaga tggcggcgaa gcgattcctc gaacgcggcg tcgacctcgc
     2101 ggccgccgag ccggtcgacc gccaggaaga ccgtatgcca gcttccggtc cagcgccgct
     2161 tggcctgggc gcgctggacg tcgtcatgct ccatcgccgc ggccgcataa tctcccggag
     2221 tcaccgcccg ccgctgccgc cggaaggcga agggcgcggc gatccggatc gcggtggtcg
     2281 gctcggcatc gagcccgccg gaggccgcga ccgggttgcg gatgccggcg atcagcggcg
     2341 ccccggcggc gacgacatgg ccgatgccgt cggccgcgac attgccgcgg cgcccgccgc
     2401 cgacccggat gcgcgcctgg aagacggcgc cctcggacgg cagccggccc gcggcgccgt
     2461 cgccgaacag cacataggcg ccgccgcctt ccgccggctc gaccttcacg gccggagtga
     2521 agcggtcggt cgcgagcaga tcggggacgg cggcccagct ctccccgtcg ccggcgagcc
     2581 gcacctgggc caccgcctcg gccggatcgg gcgcgagcgc cgccgccgcc gaccgctcga
     2641 cggcttcctc gctttcccag gccggcgcgt agacgacgga gccggcgtcg agccggtagc
     2701 gcaggcgggt cccggcgccg tcgtccggga gcaggccggt atcgtccggg ccgcccaccg
     2761 cgatcgcgtc ggcgccggcc tggttgggcg cgaggccgta gtcgatcgtt cggccctcgt
     2821 cggcgagcac gacgttgccg atggcgacgg cggcggggac gcccgcctcc tcgcgaagcg
     2881 tcagcgggaa gcgcagggcg tcctcctcgt gccagtcgac ccggacgacg gtgactccca
     2941 tcacctcgtc cttcgcctcg accggatcgg cggcgaggcg aacgacctgg cggtgcgagg
     3001 gatcgggcgg atcgagggcc gacctgccga acgggatgcg ctcctcgagg atcagcaggt
     3061 cgccccgggc gaggccgagc gccgccttgg tgccgaccag ccaggccgcg gtcgatccgg
     3121 gcagcaggca gcaggcgtcg tcgccccagt cgtgaagctc gatctcgttg cgggcgaccc
     3181 ggatcgagtc gaccggatcg agggtttcga agacgatgga tccggcgctc accagcgcct
     3241 cgaactgctc cggatcgggc ggcaggaccg ccggcacgct gccggagatc tgaagcggcc
     3301 ggctgagcag cctcgtgccg gcgggcagga gcggcgtgcc cgaatcgtcg ttcgccgcct
     3361 cgatcgccac ggcgacccgc gcgttgcagc cctccgaggc ggcgtagccg agcagccggg
     3421 cgtggcggag caccgactgg cgcagccgcg cccggccgag gaaggcctcg gtatggacgg
     3481 cgtcctggaa ccaggaggtc tggtcggcgg cgtgggcgag cgcctcgacc agggtgatgc
     3541 cgacgtcggc ggcgttgcgc tccgtccagt ccggcagcgt cgtcgccatt cggtcgagca
     3601 tcagctggcc gaagctggca tagtccctgg cgagatagtt gagcggcggg ccgaagtcgc
     3661 ggcgcgggac ttccggatcg ggcgcctcgc agtcgaaatc ggacgggcat tcgaccttga
     3721 agtggaagtc gacggcggcg agcatccggt cgatgaaggg cggcgggttc ggattgtcgg
     3781 cgccgacctg gacggcgagg cggtaggtgg agaaatcgcc gtagcggtcg agcgtcagcc
     3841 ggagggtgcg gtcgccggcg cccttggcga cctgggtgac gcggacgttg cggacccggc
     3901 tgccgccctc gatccggaaa ttgtcgggat cgagcagggc ggcggcgccg gcgacgactc
     3961 cgtcggggcg gatgaaggtg atgtcgagca ggcgctggcg gagcgcctcc gacggagcgt
     4021 cgcggtcgat cacctcgaga tagtcgatcc cgttcagcag ctccccgcct tcggtctgga
     4081 tggtcgcgtc ggcgacgcgc tgccggcgcc ggccggtgcc gcaatgctgg atcgcgccgc
     4141 cgctcatacc gccctcgtca cgttgacgct gcgctcgacc ccttcgtcga gcgcccggta
     4201 gcggacggtg atcgagagca ggctgtcgcg gctgaaggtg tcgacgccgc ggatctcgat
     4261 cacgcccgac agccattgct ggagcgcgga ctggaccatg tgctgcgccg ccgcggccat
     4321 ctcgggggcg ttctcggcga agatcagctc ggcgacgccg gcgccgaaat cggggcggtt
     4381 cacgcgctcg ccgcggcggg tgaagaggat cgcctcgatg aggtcgcgga catgctcttc
     4441 ggagtcggcg cgcgccgtcg cgccgtcggc ggcgacccgg taggggaagg cgaagtggac
     4501 ggggccgcgc gcggtcatgt cgcgagcacc ttggactgga tgttgcccca gaccgccggc
     4561 ccctgcggga tctggtcggc ggactgggcg atgtcggccg ggttcatcag cagcaccgcc
     4621 ttgttctcgg cgagcacctt ggtcgccgtc ttggtgagga ggacggtggc gcagggctgg
     4681 ggcttcgacc cgaccatgaa cgggcagccc gccaccgtcg gcttgtcgcc catgaccagc
     4741 ggcggcatcg aatcgaccag caccttggag gcggagctga ccagcgtcgc gggtatggcg
     4801 tgcgggcact ggatcatggt cgcggtggtg acgacggcga ggctcatggc agcaccttca
     4861 ggttggagcc gttgatcgag accccgtcga ccgagagctt gacgctgctc ttgccccatc
     4921 tgagctcgag ggcggtcgcg tcggcggcca gcttggcctc gccgccgggg gtggtgaggg
     4981 tgatctcgag ggtcccggcg ccgggcatgt cgaccacctc gatccggaag ttgtcgcccg
     5041 cccagacccg ggtcatcgcc tgggtcgggc ccatcggcgc gggcgcctcg ccggtgcccc
     5101 agaagccgcc cgaccagatc ggatagtcga ggtcgccgcg ctcgaactcg acccagacct
     5161 tggctccgac cggggggatg acgaagattc cgacgtcggg gccggcgccg ggcaggcagg
     5221 gcatcgccca ggcgagcgtg ctgttgtcga gcaggcccgg gacgctgacc tggatccggc
     5281 ccaggccgag cgggtcgagg ttgttctgga cctcgccgcg gaacttgccg aagcagctca
     5341 tgaccgcacc gccaggctgg cggcgccgag ctcgccgcgg gtgaggacga agccctgctc
     5401 atattcgccc gggcggatcc ggtggcggac ctcggcgacc ttgtagaggc cgtcgaacat
     5461 cgcgcccgcg ccgcgcaggt agatctggtc gcgggccttg agggcgccgt tgtagcgcgt
     5521 cgaatcgacc gtcccggtca ccctgacgac ccgccgcgcg ctctcgtcca ccgccccctg
     5581 ggcgcgcgcc atcgcctgag cgaaggtgag gccgctggtc tcgatcgggg tggtgcgggt
     5641 cttgcccatc cgggccgccg cctcgggcag ggcgccgagc ggggtctggc tcggagtcac
     5701 cgccttgacc ggcatcgtcg ccccggtctg gcggtcctgc acctgcgtct cgacggtggt
     5761 gagctcctcg gcgctgttgg cggcgcggat gtcgtaggcg tcggtgaccg gcccggagct
     5821 gaccgtcaac gtccgctgcg gagctccggg acgcaccggc ggcccccagt agaggatgtt
     5881 ggtgagcggc accggtccgg cctcgacata ggtctcgtag ccgtggcgcc gggccatggc
     5941 gttgagatag tcccagtccg agcagcgctg ctgcggggtg cgctcgaccg gcagcggcgg
     6001 atcgatgacc ttgggcggca tcaccatcgg aatgatcccg aactgggcgt aggagagcgc
     6061 gatcaggttc gcgatcgtca tctcgtccat cgccgggcgc tcgacgttct tcacctcccg
     6121 gtccatctcg aagctgagat cgtggccgag caggacgatc cggccctggc tggagccttc
     6181 ccccggggag acgtggcgct cgctgacgag gccgtcgaag atgacggtgg gaacggcgtc
     6241 gaacaggatc gacacgacca gccgcgaccc gcggttgaag cggggatcgg cgaccagggg
     6301 cgtctcgagg aaatcggccg ggcctgaccg tccggcacga agcaccatcc ggaagcccga
     6361 cggctcgcgg tccgagagcc ggatgtcgag ctcctccagc gcctggaggg cctccgccgg
     6421 catcggcagg gcgaccggac tcggcccgat caggatgttg gccttgatgc cgagcaggtt
     6481 gccgagaagg ctcatccgac gaggctgacc gttccggcca cgggaacgag gacgacatcg
     6541 cccggcgcgc ccggcagagc gctgggctcc atcgcccggt tcgcgtcggc gatctgccac
     6601 caggcggtgg gcgcccccag attcctccag gcgagcagat cgatccggtc gctgtcggtg
     6661 actcgatggc gaaggacggg ctggaagccg tccggctgcg gcaatatgcg gggcgcgagg
     6721 taggtcaccg gctgcccgct cgaagtgacg tagacgagct ggggcgccga tgcgtagcgg
     6781 ctgtctgcgg cgatcatcga gactcccttc tctcttcaga acaggtcggc gctccccccg
     6841 agc
//
LOCUS       DASVQZ010000117         5532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_793967, whole genome shotgun sequence.
ACCESSION   DASVQZ010000117 DASVQZ010000000
VERSION     DASVQZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793967"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VF547_03245"
     CDS             complement(<1..438)
                     /locus_tag="VF547_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEX8261871.1"
                     /translation="MSLAKKILTLAAPAALLMLGGCATGLPTQVSRYQAMPAPAGQSF
                     VIQPADPAERGGLEFSRYADLVRRHMIEQGYSEAPSPQAASLIVSLDYGVDDGRQQVV
                     TRPALGYSRFGYGGFGYRPYWSRYGGYGRFRSPFYYGWDDPFWY"
     gene            complement(657..2213)
                     /gene="murJ"
                     /locus_tag="VF547_03250"
     CDS             complement(657..2213)
                     /gene="murJ"
                     /locus_tag="VF547_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX8261872.1"
                     /translation="MNLWKATGTIGGLTMVSRILGFAREMVFARVMGAGMAADAFALA
                     FLIPNLFRRLFGEGAFSQGFVPLFSQRLHGEGGMEEARRFAEEVQAVFLPMLMAITAV
                     FVIFMPAFVAVIGSGWRDEPAKFALAVELSRITFPYMLLISMVSLFSGVLNSLTRFTA
                     AAFAPALLNAALIGSLLLVPAGGTVTARAMAWGVVAGGVLQFALCWVAVRRAGVKLSL
                     RAPKMTPRVKELLILILPATLGGGIYYISQLFYAYFATRVGEGTLVYLGFADRLNQLP
                     LAIIGSALGTAILPTISRHVGAGDGAGAARVQGQATELAMLLTLPAAVALAVTAGPIV
                     AALFQGGKFTAEDAATTSLVLSIIVAGLPAYVLIKVLTPGFFARKDMKTPVIIAAGSL
                     AIGVALNFALIPVIGIAALALTTALSAWINALSLYVLLRARGHFRIEPWLWSRLVRQL
                     IAALAMAAALYLVREQREGFFAGSAGRRLIGVAALVSVGGIVYFAVAWTIGAMNRDDI
                     LILLRRKKAG"
     gene            complement(2239..2751)
                     /gene="secB"
                     /locus_tag="VF547_03255"
     CDS             complement(2239..2751)
                     /gene="secB"
                     /locus_tag="VF547_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX8261873.1"
                     /translation="MAEEQGSPVEPQQTNGNDTSPQVGVISQYVKDLSFENPNAPAVY
                     QWQSQPKIEVDFNIGSSKLNDEIYEVVLRIEVRATADGQTAFAVDLMYAGLIGMRNVE
                     EQDSQGFLLAEAPRILFPFARHIVAQTVQEGGFPPLMLDPIDFHALYAARAAQESAGA
                     GTPAGEIGQA"
     gene            2932..3582
                     /locus_tag="VF547_03260"
     CDS             2932..3582
                     /locus_tag="VF547_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX8261874.1"
                     /translation="MTEIVLLAMVALFVGLRLYSVLGRRTGHEQQPILRPAETGTAAE
                     APAAAADVPVDRPEAGGFVYEEPATPGIRAIVGADPSFDVGRFRDGAQAAYRMILEAF
                     WRGDREELRHLVAGEVLASFEEAIGAREAAGEQLDNRLVQIERAVIQDARLAGRIAEI
                     DVRFDADIAAVTRDREGKVIAGTLSDAIPTHDVWTFRRDLASRDPNWLLVETDEAD"
     gene            3705..4802
                     /locus_tag="VF547_03265"
     CDS             3705..4802
                     /locus_tag="VF547_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEX8261875.1"
                     /translation="MPQPQPPPAAEVKPPTAAELGVSAGPALPAIDAAAAARALEAFR
                     ISCPALLQRSDPSGLTRPEDWREACSAAGPADTRSASAFFAEHFETVRVGDGAAFVTG
                     YYEPEIAGSRTRQPGYEVPVYRKPPDLVEVDAATAEAAGTPRRGRLENGVIVPYYDRA
                     EIEDGALAGRGLEIAWAADPIDLFFLQIQGSGRLRLPDGDVMRIGYAGQNGRDYVGIG
                     RLMRERGLLEPGRTTMQDLVAWLRAHPEEGRAIMRENKSYVFFQELTGAGPLGALGLP
                     VTARATVAADPKFVPLGAPVILELDRTEANGLWIAQDTGGAIKGSNRFDTFWGAGVEA
                     ARIAGGMQGRGRAWLLLPKGTLARLHAPSQP"
     gene            4783..5313
                     /locus_tag="VF547_03270"
     CDS             4783..5313
                     /locus_tag="VF547_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX8261876.1"
                     /translation="MRRLNPDEAKLWARVVESVRPLGAAPSEPATAPVEGPAPQAAPP
                     APRLRREPVRPLPAARSGHGDSLDGTWERRLGRGLVAPDATLDLHGHNLATAYDLLDR
                     RLDEAIRAGARLLLLVTGKPRGEDGSAKRGAIRAAVGDWLHASRHAGRIAAVRNAHPR
                     HGGAGALYIVLRRPRS"
BASE COUNT          874 a         1919 c         1974 g          765 t
ORIGIN      
        1 ataccagaag ggatcgtccc agccgtaata gaagggcgac cggaagcggc cgtagccgcc
       61 gtagcgcgac caatagggcc ggtagccgaa gccgccgtag ccgaagcggc tgtagccgag
      121 cgccggccgg gtgacgacct gctggcggcc gtcgtcgact ccatagtcga gcgagacgat
      181 gaggctcgcg gcctgcgggg acggcgcctc gctatagccc tgctcgatca tgtgccgccg
      241 aacgaggtcg gcatagcggg agaattcgag gccgccgcgc tcggccggat cggccggctg
      301 gatgacgaag ctctggcccg cgggggccgg catcgcctgg tagcgggaga cctgggtcgg
      361 aaggcccgtc gcgcagccgc ccagcatcag cagggccgcg ggcgcggcga gggtcaggat
      421 cttcttggca agggacatgg aacgcatcct cttcgagaac agggccgcag ctctaactat
      481 ataggaaacg gcgccttttt acaaaagggg gctgaatggc gaatgaaggg ggcaagtgcg
      541 gcgggcgatc ctccacccgt tcgggctgag cctgtcgaag cccttcactc ctatcggaaa
      601 aaaaggcgag gccttcgaca ggctcaggcc gaacggaaat cgggagctgc gcccccttac
      661 cccgccttct tgcgcctgag aaggatcagg atgtcgtcgc gattcatcgc accgatcgtc
      721 caggcgacgg cgaaatagac gatgccgccg acggagacga gcgcggcgac cccgatcagc
      781 cggcgcccgg cggagccggc gaagaagccc tcgcgctgct cgcgcaccag gtagagcgcc
      841 gcggccatgg cgagcgccgc gatcagctgc cttaccagcc gactccacag ccaaggctcg
      901 atccggaaat gtccgcgcgc ccggagcagg acgtagaggc tgagcgcgtt gatccaggcc
      961 gagagcgccg tggtcagggc cagggccgcg atgccgatga ctgggatcag cgcgaagttg
     1021 agcgcgaccc cgatggcgag gctgcccgcc gcgatgatca ccggcgtctt catgtccttg
     1081 cgcgcgaaga agccgggggt gagcaccttg atcaggacat aggcgggaag gccggcgacg
     1141 atgatggaca gcaccaaaga ggtggtcgcg gcgtcctcgg cggtgaactt gccgccctgg
     1201 aagagcgccg cgacgatcgg ccccgccgtc accgccagcg ccaccgcggc gggaagggtg
     1261 agcagcatgg cgagctcggt cgcctggccc tgcacccgag ccgcgccggc gccgtcgccg
     1321 gcgccgacgt ggcggctgat cgtcggcagg atggcggttc ccagcgcgga gccgatgatc
     1381 gcgagcggaa gttggttgag ccggtcggcg aagccgagat agacgagcgt gccctcccct
     1441 acccgggtcg cgaaataggc gtagaagagc tggctgatat agtagatgcc gccgccgagc
     1501 gtcgctggca ggatcaggat cagcagctcc ttgacccgcg gggtcatctt cggagccctc
     1561 aggctgagct tcacgccggc gcggcgcacc gccacccagc acagggcgaa ctggagcacc
     1621 ccgccggcga cgactcccca ggccatcgcc cgggccgtca ccgtcccgcc ggccgggacg
     1681 agcagaagcg agccgatcag ggcggcgttg agcaaggcgg gggcgaaggc ggcggcggtg
     1741 aagcgggtca gcgagttgag caccccggag aagagcgaca ccatcgagat gagcagcatg
     1801 taggggaagg tgatccgcga cagctcgacg gccaaggcga acttggccgg ctcatcgcgc
     1861 cagcccgagc cgatcacggc gacgaaggcg ggcatgaaga tgacgaacac cgccgtgatc
     1921 gccatcagca tcggcaggaa caccgcctgc acttcctcgg cgaagcgccg ggcttcctcc
     1981 atcccgccct caccgtgcag gcgctggctg aacaaaggca cgaagccctg gctgaacgcg
     2041 ccctctccga acagccggcg gaagaggttg gggatgagga aggcgagggc gaaggcgtcg
     2101 gcggccatgc cggcccccat cacccgggcg aagaccattt cgcgcgcgaa gccgaggatc
     2161 cggctcacca tggtgagccc gccgatcgtc ccggtcgcct tccagaggtt catggtgccg
     2221 gcgctccgga ccggagcgtc aggcctggcc gatctcgccg gccggcgtgc ccgctccggc
     2281 cgattcctgc gccgcccggg cggcgtagag cgcgtggaag tcgatcggat cgagcatcag
     2341 cggggggaag ccgccctcct gcacggtctg ggcgacgata tggcgggcga aggggaacag
     2401 gatgcgcggc gcctcggcga gcaggaaccc ctgcgaatcc tgctcctcga cgttgcgcat
     2461 gccgatcagg ccggcataca tcaggtcgac cgcgaaggcg gtctggccgt cggcggtggc
     2521 gcggacctcg atgcgcagca ccacctcgta gatctcgtcg ttgagcttgc tcgagccgat
     2581 gttgaagtcg acctcgatct tcggctggct ctgccattga tagaccgccg gcgcgttcgg
     2641 attctcgaac gagaggtctt tgacatattg ggagatgacg ccgacctgcg gcgaggtgtc
     2701 gttgccgttg gtctgctggg gctcgacggg gctgccctgt tcttcggcca ttttgattcg
     2761 ctttcgaatg aggtggagcg gggtcgcggg ggcgcggaac ccgtgcgccg cggcgggtag
     2821 caggcgcggc ccggccgcgc aaccgctcgc cacggccgat tgcaaggcgg cgccatttga
     2881 atccttgccg ccaacggcct atgtagaggt tcgtagagtt cggagatcgt agtgaccgag
     2941 attgtcctcc tggcgatggt ggccctgttc gtgggcctga ggctttactc ggtgctgggc
     3001 cggcgcaccg gccatgagca gcagcccatc cttcgccccg ccgagaccgg aacggctgcc
     3061 gaagccccgg ccgcggccgc ggacgtcccg gtcgaccggc ccgaggccgg cgggttcgtc
     3121 tacgaggagc cggccacgcc cggcatccgg gcgatcgtcg gcgccgaccc gagcttcgac
     3181 gtcggccgct tccgggacgg cgcccaggcc gcctaccgga tgatcctcga agccttctgg
     3241 cgcggcgacc gcgaggagct tcggcacctc gtcgccggcg aggtcctggc atcgttcgag
     3301 gaagcgatcg gcgcgcgcga ggcggcgggc gagcagctcg acaaccggct ggtccagatc
     3361 gagcgggcgg tcatccagga tgcccgcctc gccgggcgga tcgcggagat cgacgtgcgc
     3421 ttcgatgccg acatcgccgc cgtcacccgc gaccgcgagg gcaaggtcat cgccggcacg
     3481 ctgagcgacg cgatccccac ccacgacgtc tggaccttcc gccgcgacct cgccagccgc
     3541 gacccgaact ggctgctcgt cgagaccgac gaagcggatt gaggccggtg ggggtcgggg
     3601 ggattttttc tgcttcaggc aagccgaacc ttctgaaact ttcgccgctg ctggcggcga
     3661 tgatcgtcct cgccagctgc gtgcccaggc agcagcggcc cccgatgccc cagccgcagc
     3721 cccccccggc ggcggaggtc aagcctccga ccgcggccga gctgggggtc tccgccggcc
     3781 cggcgctgcc ggccatcgac gccgccgccg cggcccgggc actggaggcg ttccggatca
     3841 gctgcccggc ccttctccag cgcagcgacc ccagcggcct cacccggccc gaggactggc
     3901 gggaggcctg cagcgccgcg ggcccggccg acacgcgttc cgcatccgcc ttcttcgccg
     3961 agcatttcga gaccgtccgg gtcggcgacg gggcggcctt cgtcaccggc tattacgagc
     4021 ccgagatcgc cggctcgcgc actcggcagc ccggctatga agtgccggtc taccgcaagc
     4081 ccccggacct cgtcgaggtc gacgcggcca cggccgaggc ggccggcacg ccccgccggg
     4141 gacgactcga gaacggggtg atcgtgccct attacgaccg cgccgagatc gaggacggag
     4201 cgctcgccgg ccgcgggctg gagattgcct gggcggccga cccgatcgac ctgttcttcc
     4261 tccagatcca gggctccggc cgactgcgcc ttcccgacgg cgacgtcatg cggatcggat
     4321 atgcggggca gaacggccgg gactatgtcg gcatcggcag gctgatgagg gagcggggac
     4381 tgctcgagcc gggccggacg acgatgcagg acctcgtcgc ctggctgcgc gcgcatcccg
     4441 aggaggggcg ggcgatcatg cgcgagaaca agagctacgt cttcttccag gagctgaccg
     4501 gcgcaggccc gctcggcgcc ctcggcctgc cggtcacggc ccgggccacc gtcgccgccg
     4561 atcccaagtt cgtgccgctc ggcgccccgg tgatcctcga gctcgaccgg accgaggcga
     4621 acggcctgtg gatcgcccag gatacgggcg gcgcgatcaa gggctccaac cgcttcgaca
     4681 ccttctgggg cgcgggcgtc gaggcggctc gcatcgccgg cggcatgcag ggccgcggcc
     4741 gcgcctggct cctgctgccc aaaggcacgc tcgcccgact ccatgcgccg tctcaaccct
     4801 gacgaggcga aattatgggc gagggtggtc gagtcggtgc gcccgctcgg cgccgccccg
     4861 tccgagcccg ccacggcgcc cgtggagggc ccggccccgc aagcggctcc gccggctccg
     4921 cgtctccgcc gggagccggt ccggccgttg ccggcggccc ggtccggcca cggcgattcg
     4981 ctcgacggca cctgggagcg gcgcctcggc cgcgggctgg tcgcgcccga tgccactctc
     5041 gatctgcacg gccacaacct cgccaccgcc tacgaccttc tcgaccggcg gctggacgag
     5101 gcgatccgcg ccggcgcgag gcttctgctg ctcgtcaccg gcaagccgcg gggcgaggac
     5161 gggtcggcga aacgaggcgc cattcgcgcc gcggtcggcg actggctgca cgcctcgcgc
     5221 cacgccggcc gaatcgcggc ggtgcgcaac gcccaccccc gccacggcgg tgcgggcgcc
     5281 ctgtacatcg tccttcgacg tccccggtcc tgacggatac gaaatggtaa cgcgtccggc
     5341 ataaagtgcc gctgaatgcg cgactgtttg gggaccgcgc cacagaccgg acaggaacgc
     5401 cgccgatcat gtcttcgacg gttggaaaga tcagcaatcg ggtgctctcc gtcctggcgg
     5461 gcgtgtcggc cttcgccctc accatgctcg gcttcctcct ggtcggcacg ctcgaccagc
     5521 aggtcgtcgc ct
//
LOCUS       DASVQZ010000118         6186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_410651, whole genome shotgun sequence.
ACCESSION   DASVQZ010000118 DASVQZ010000000
VERSION     DASVQZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410651"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /gene="hemC"
                     /locus_tag="VF547_03275"
     CDS             complement(<1..479)
                     /gene="hemC"
                     /locus_tag="VF547_03275"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX8261877.1"
                     /translation="MQPLLRLGTRGSPLALVQARLVATALRDAHGWGPELIEIEPIAT
                     SGDQLRDRPLAEMGGKALWTRELDLALLEGRIDLAIHSMKDVETIRPPELAIAAMLPR
                     ADVRDRLVGASSLEALPDGARVGTSSPRRAAQLLARRPGLAILPFRGNVETRLRKIDL
                     "
     gene            524..1558
                     /gene="tsaD"
                     /locus_tag="VF547_03280"
     CDS             524..1558
                     /gene="tsaD"
                     /locus_tag="VF547_03280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX8261878.1"
                     /translation="MSLVLGIESSCDETAAALVASDRRILAHRLAGQEGAHGPYGGVV
                     PEIAARAHVEILPALIEQALAEAGTELAEVDAIAATAGPGLIGGVMVGLVAAKGLALA
                     AGKPLVAINHLEGHALSPMLGDPGLAFPYLLLLVSGGHCQLLSVEALGRYHRLATTMD
                     DAAGEAFDKTAKALGLGFPGGPAVERSARTGDPAAVPLPRPLLGSAEPHFSFAGLKSA
                     VLRAREAGRYSAEDIAASFQQAVLECLVDRTRRALDRAPDSTALVVAGGVAANSAIRS
                     ALASLAEARGLPFVAPPLWLCTDNAAMIAWAGALRFDSGLTDPLDAPARARWPLDPAA
                     EKVRGAGVKA"
     gene            1689..2678
                     /locus_tag="VF547_03285"
     CDS             1689..2678
                     /locus_tag="VF547_03285"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8261879.1"
                     /translation="MTDISTIGVVGGGAWGTALAQVAAGGGETLLWAREPEVVESVNA
                     AHENRLFLEGIPLDPAIRATADLADLAACGALLVVTPAQHMRSVLAAMPRLTAPLILC
                     AKGMEEATMELMHDVAREAQPQAPVAVLSGPTFAHEVAAGLPAAVTLAVEDPGLGERL
                     RARIARPWFRPYLSDDVAGAEIGGAVKNVLAIACGVVEGRRLGQNARAALISRGFAEM
                     TRFGLARGARAETLAGLSGLGDLVLTCSSTSSRNFSLGKGLGEGRSPAELLADRRTVA
                     EGAHTAPVLARAAKAAGVDMPIVEAVCALLAGEASVDKVVERLLSRPLRSEGI"
     gene            complement(2821..3015)
                     /locus_tag="VF547_03290"
     CDS             complement(2821..3015)
                     /locus_tag="VF547_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEX8261880.1"
                     /translation="MSRPSSIAGPSRRPPHVEAPAHLSQSPPVPEPGPPVQGAESGGP
                     KGQEPTRYGDWENKGICWDF"
     gene            3053..3967
                     /locus_tag="VF547_03295"
     CDS             3053..3967
                     /locus_tag="VF547_03295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8261881.1"
                     /translation="MNRFRTLMLLAALTALFMGLGFTIGGPRGAVLALLVAAAMNLVT
                     FWNADRIVLSMHGAKEMTVGDCPQLVGMVHALAARAGLPDPRVYLIDSPHPNAFATGR
                     DPAHAAVAVTTGLLGILSREEVAAVMAHELGHVRNRDTLVMTMTATIAGAISMLANFG
                     FFFRGDGGRGNPLAMIAALFLAPFAAMLVQLAISRTREYGADRAGAEICGDPAALASA
                     LAKLHEGAARIPSPVAARVPAAASLYIVPSGGGRDSLFATHPATENRIAALRAMAGEL
                     AAQQPRPPGPRARRAGASALDPLGRRHR"
     gene            3964..5223
                     /locus_tag="VF547_03300"
     CDS             3964..5223
                     /locus_tag="VF547_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX8261882.1"
                     /translation="MNRPADPAGVPARRAALSLLDAVLRKGLPLEAALDTAARGLPAN
                     DRGLAHAIAAEALRRLPALDALIDGATRRPLPDDAKARAVLRIALVQALALDTPPHAA
                     ISTALPLVEGGPRKLVHGVFGTLMRQKAALPALPSLPAAVEARWTRNWGEAVVAEAAT
                     LLAAPPPLDLCFASGTGPPPEMEGVSLMPGHLRLPARSAVAGLTGYGEGGWWVQDIAA
                     SLPARLLGEGRGRSALDLCAAPGGKTMQLASAGFEVTAVDSSESRLARLSENLARTNL
                     RAKVIAADILRWSPPEKVDAILLDAPCSATGIYRRHPDVLHRVRPRALAELAEGQKAM
                     LARAAGWLKPGGTLVYSVCSLEPEEGEAVADAFLAERRDFEAAHIEAGELPGTIAVEG
                     SRLRTLPGLFAEAGGADGFFIARFRRV"
     gene            5336..5995
                     /gene="rpe"
                     /locus_tag="VF547_03305"
     CDS             5336..5995
                     /gene="rpe"
                     /locus_tag="VF547_03305"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX8261883.1"
                     /translation="MQQPVRIAPSILSADFARLGEEVRAVDEAGADWIHVDVMDGHFV
                     PNLTIGPAVVKALRPHSARPFDVHLMISPADPFLEAFAGAGADILTVHPEAGPHLHRT
                     LQAIRALGKRAGVALNPATPAKMLDYVLEEVDLVLVMSVNPGFGGQSFVDSQLRKIEA
                     VRKQIDKAGLAVDLEVDGGIDPSTARRAIDAGADVLVAGTATFRGGPPSYAANIAGLR
                     R"
     gene            5992..>6186
                     /locus_tag="VF547_03310"
     CDS             5992..>6186
                     /locus_tag="VF547_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HEX8261884.1"
                     /translation="MSLLEKIDPPEAGEIEPGRRLIRAGGGQGASLGEHLSSMLHRLS
                     WRTPFHAFRLRGRYPLKLLAV"
BASE COUNT          765 a         2312 c         2213 g          896 t
ORIGIN      
        1 aggtcgatct tgcgaagccg ggtctcgacg ttgccgcgga agggaaggat ggcgagtccc
       61 ggcctgcgcg ccagcagctg cgccgcccgc cgcggcgagg acgtgccgac ccgggcgcca
      121 tcgggcaggg cctcgaggct cgacgccccg accagccggt cgcgcacgtc ggcccgcggc
      181 agcatcgcgg cgatggcgag ctcgggcggc cggatcgtct cgacgtcctt catcgaatgg
      241 atggcgaggt cgatccgccc ctccagcagg gcgaggtcga gctcccgcgt ccacagcgcc
      301 ttgcccccca tctcggccag cggccggtcg cgcagctggt cgccgctggt ggcgatcggc
      361 tcgatctcga tcagctccgg tccccagccg tgggcgtcgc gcagcgccgt cgcgacaagc
      421 ctcgcctgaa cgagcgccag cggcgatccg cgggttccta gacgcaagag cggctgcatg
      481 cgccgcttgt gctagcgggt gcgcgggtgg cagggaagcg cgtatgagcc ttgtcctggg
      541 catcgaatcg agctgcgacg agacggcggc ggccctggtc gcgtcggacc ggcgcattct
      601 cgcccatcgc ctcgccggcc aggagggcgc ccacggcccc tatggcggag tggtcccgga
      661 gatcgccgcc cgcgcccatg tcgagatcct gcccgctctg atcgagcagg ccctggccga
      721 ggccggaacc gagcttgcgg aggtggatgc gatcgccgcg accgccggcc ccggcctgat
      781 cggcggagtg atggtcggcc tggtcgcggc caaggggctg gcgctcgctg ccggcaagcc
      841 gctcgtcgca atcaaccatc tcgagggcca cgccttgtcg ccgatgctgg gcgatccggg
      901 cctcgccttc ccctatctgc tcctgctcgt ctccggcggc cattgccagc tgctgtcggt
      961 cgaggcgctc ggccgctatc atcggctggc gaccacgatg gacgacgcgg cgggcgaggc
     1021 gttcgacaag acggccaagg cgctcggcct cggcttcccc ggcggcccgg cggtcgagcg
     1081 ctcggcccgg acgggcgatc ccgccgccgt cccgctgccg aggccgctcc tcggctcggc
     1141 cgagccgcat ttctcgttcg ccggcctcaa gagcgcggtg ctccgggcgc gggaggcggg
     1201 gcgctactcg gccgaggaca tcgccgcctc gttccagcag gccgtcctcg aatgcctcgt
     1261 cgaccgcacc cgtcgcgcgc tcgaccgggc accggattcg acggcgctgg tcgtcgccgg
     1321 cggggtggcc gcgaactcgg cgatacgttc ggccctcgcg tcgctggccg aggcccgcgg
     1381 cctgcccttc gtcgctccgc cgctgtggct gtgcaccgac aatgcggcga tgatcgcctg
     1441 ggcgggcgcc ctgcgcttcg attcgggcct caccgatccg ctcgacgcgc cggcccgggc
     1501 gcgctggccg ctcgatccgg cggccgagaa ggtgcgcggc gccggagtga aggcgtgaac
     1561 ctgtccacac cgtgcgggcg gagccccatg cttccgttcg gcctgagctt gtcgaagccc
     1621 gttctttcct tgcgggcctg gggaaaggga agggcttcga cgggctcagc ccgaacgggg
     1681 gtttcgcggt gacggacatt tccaccatcg gtgtggtcgg cggcggggcc tgggggacgg
     1741 ccctcgccca ggtcgccgcc ggcgggggcg agaccttgct ctgggcccgc gagccggagg
     1801 tggtcgagag cgtcaacgcc gcgcacgaga accgcctgtt cctcgagggc atcccgctcg
     1861 acccggcgat ccgcgccacc gccgacctcg ccgacctcgc cgcctgcggg gcgctgctcg
     1921 tcgtcactcc cgcccagcac atgcgctcgg tgctggccgc gatgccccgg ctcaccgctc
     1981 cgctcatcct ctgcgccaag gggatggagg aggcgacgat ggagctgatg cacgacgtcg
     2041 cccgcgaggc gcagccccag gcgccggtcg cggtgctgtc cggcccgacc ttcgcgcacg
     2101 aggtcgccgc gggcctgccc gcggcggtca ccctggcggt cgaggatccg ggactgggcg
     2161 agcggcttcg ggcgcggatc gcccggccct ggttccggcc ctatctgtcc gacgacgtcg
     2221 ccggcgccga gatcggcgga gcggtcaaga acgtcctcgc catcgcctgc ggcgtggtcg
     2281 aagggcggcg gctcggccag aacgcccggg ccgcgctgat ctcgcgcggc ttcgcggaga
     2341 tgacccgctt cggcctcgcc cgcggcgcac gcgccgagac cctggccggc ctctccgggc
     2401 tcggcgacct cgtcctcacc tgctcatcga ccagctcgcg caacttcagc ctcggcaagg
     2461 ggctcggcga gggacggagc ccggcggagc tgctcgccga ccggcgaacc gtggccgaag
     2521 gcgcgcacac ggcgccggta ctggcgcggg cggcaaaggc ggccggggtc gacatgccca
     2581 tcgtggaagc ggtgtgcgcg ctcctcgccg gcgaagccag cgtcgacaag gtggtcgagc
     2641 ggcttctgtc caggccgctg agaagcgagg gaatttaatt cctcccccgg atttccgggg
     2701 gagggggacc gcgcgaagcg cggtggagag ggctgcggac tcggaaaggc cccgccacca
     2761 tgcttcgcat ggtccccctc ccctgcaaat gcagggggag gaatttggcg gcgcgacgcc
     2821 tcagaaatcc cagcagatgc ccttgttctc ccagtcgccg tagcgggtcg gctcctggcc
     2881 cttgggaccg ccgctttccg caccctgcac cggcggcccc ggctccggca ccggcggact
     2941 ctgcgacagg tgggccggcg cctcgacatg cggggggcgc cgcgacggac cggcgattga
     3001 agaaggcctg ctcacccccc acatttcggg cctggaaggc ggacgcgcaa ggatgaaccg
     3061 gttcaggacc ctgatgctgc tcgcggcgct gacggcgctg ttcatggggc ttggcttcac
     3121 catcggcggc ccgcgcggcg ccgtcctcgc ccttctggtc gccgccgcga tgaacctggt
     3181 caccttctgg aacgcggacc ggatcgtgct gtcgatgcac ggcgccaagg agatgacggt
     3241 gggggactgc ccgcagctgg tcgggatggt ccacgccttg gcggcgcggg ccggcctgcc
     3301 cgatccgcgg gtctatctga tcgattcgcc ccatcccaac gccttcgcca ccggccggga
     3361 cccggcgcat gcggccgtcg ccgtcaccac cggcctgctc ggcatcctca gccgcgagga
     3421 ggtggcggcg gtgatggcgc acgagctcgg gcacgtccgc aaccgcgaca cgctggtgat
     3481 gacgatgacc gccacgatcg ccggggcgat ctcgatgctg gccaatttcg gcttcttctt
     3541 ccgcggcgac ggcggccgcg gcaatccgct ggcgatgatc gccgcgctgt tcctcgcgcc
     3601 gttcgcggcg atgctggtcc agctggcgat cagccgcacc cgcgaatatg gcgccgaccg
     3661 cgccggcgcc gagatttgcg gcgatcccgc cgccctcgcc tcggccctcg ccaagctgca
     3721 cgaaggggcc gcccggatcc cgagcccggt cgcggcccga gtccccgccg cggcctccct
     3781 ctacatcgtt ccgtccgggg gcggccgcga cagcctgttc gcgacccatc cagccaccga
     3841 gaaccggatc gccgcccttc gggcgatggc gggcgagctt gcggcgcagc agccccgccc
     3901 gccgggcccg cgcgcccgaa gggccggcgc cagcgcgctc gatccgctcg ggcggcgcca
     3961 taggtgaacc gccccgccga tccggcaggc gttccggccc gccgagccgc cctaagcctc
     4021 ctcgacgccg tgctccgcaa ggggctgccg ctggaggccg cgctggacac ggcggcgcgc
     4081 ggcctcccgg ccaacgaccg cggcctggcg cacgcgatcg ccgccgaggc gcttcgccgc
     4141 ctgcccgctc tcgacgccct gatcgacggc gccacccgcc gcccgcttcc cgacgacgcc
     4201 aaggcgcgcg ccgtgctccg catcgcgctc gtccaggccc tcgcgctgga cactccgccg
     4261 cacgccgcca tctcgacggc gctgccgctg gtcgaaggcg ggccgcgcaa gctggtccac
     4321 ggcgtcttcg gcaccctgat gcggcagaag gcggcgcttc cggcgctgcc ctcgctgccg
     4381 gcggcggtcg aggcgcgctg gacgcgcaac tggggcgagg cggtcgtcgc cgaggccgcc
     4441 acgctgctcg ccgcgccgcc gccgctggac ctgtgcttcg cctcggggac cgggcctccg
     4501 ccggagatgg agggggtgag cctgatgccc ggccatctgc ggcttccggc gcgaagcgcg
     4561 gtggcggggc tgaccggtta tggcgaaggc ggctggtggg tccaggacat cgcggcctcg
     4621 cttccggcgc gcctgctcgg cgagggccgc ggccgaagcg cgctcgatct gtgcgcggcg
     4681 ccgggcggca agacgatgca gctcgccagc gcgggcttcg aggtgaccgc cgtcgattct
     4741 tccgaaagtc gtcttgcacg actttccgag aatctggcgc gcaccaatct gcgggcgaag
     4801 gtgatcgcgg cggacatcct gcgctggtcg ccgcctgaga aggtggatgc gatcctgctc
     4861 gacgcgccct gctccgcgac cggaatctac cgccgccatc ccgacgtcct ccatcgggtc
     4921 cgtccccgcg cgctcgccga gctcgcggaa gggcagaagg cgatgctcgc gcgcgccgcg
     4981 ggctggctca agcccggcgg caccctcgtc tactcggtct gctcgctgga gccggaggag
     5041 ggtgaggcgg tggccgatgc cttcctcgcc gagcgccgcg acttcgaggc cgcccatatc
     5101 gaggccggcg agcttcccgg cacgatagcg gtcgagggca gccgcctgcg cacccttccc
     5161 ggcctgttcg ccgaggccgg cggcgccgac ggcttcttca tcgccaggtt taggcgcgtc
     5221 tgacggctgc gctgtctgcc tccttccccc tctccccgcc ggggagaggg attttaggcg
     5281 gggataagta gtcccccttc cttgccgcgc gactcgggca gggttacggt tgggcgtgca
     5341 gcagcccgtc cgcatcgccc cctccatcct ctcggccgat ttcgcccggc tcggcgagga
     5401 ggtgcgcgcg gtggacgaag ccggcgccga ctggatccat gtcgacgtga tggacggcca
     5461 tttcgtgccc aacctcacca tcggcccggc ggtggtgaag gcgctccgcc cccacagcgc
     5521 caggccattc gacgtccatc tgatgatctc gccggccgat ccgttccttg aggccttcgc
     5581 cggcgccggc gcggacatcc tcaccgtcca tccggaggcg ggcccacatc tccaccggac
     5641 cctccaggcg atccgcgcgc tcggcaagcg ggccggggtc gcgctcaacc cggcgacgcc
     5701 cgccaagatg ctcgattacg tgctcgagga agtcgatctg gtgctggtga tgagcgtcaa
     5761 tcccggtttc ggcggccaga gcttcgtcga cagccagctt cgcaagatcg aggcggtgag
     5821 aaagcagatc gacaaggccg gcctggcggt cgacctcgag gtggacggcg gcatcgaccc
     5881 ctccaccgcc cgccgcgcga tcgacgccgg ggccgacgtg ctggtcgccg ggaccgcgac
     5941 cttccgcggc ggtccgccaa gttatgcggc gaacatcgcc gggcttcggc gatgagcctg
     6001 ctcgagaaga tcgacccgcc cgaggccggc gagatcgagc cgggccggag gctgatccgg
     6061 gccggcggcg gccagggcgc ctcgctcggc gagcatctgt cctcgatgct ccaccgcctc
     6121 tcctggcgga cgcccttcca cgcgttccgg ctgcgcggcc gctatccgct caagctgctc
     6181 gcggtc
//
LOCUS       DASVQZ010000119         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_411139, whole genome shotgun sequence.
ACCESSION   DASVQZ010000119 DASVQZ010000000
VERSION     DASVQZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411139"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VF547_03315"
     CDS             complement(<1..936)
                     /locus_tag="VF547_03315"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX8261885.1"
                     /translation="MKLVVVESPAKAKTIEKYLGAGYRVLASYGHVRDLPPKDGSVDP
                     DSGFAMEWETYADKARQLKAITDEAKKADSLILATDPDREGEAISWHVQEVLRKRKAL
                     PADVERVTFNAITKATVTEAMKHPRALDEDLIDAYRARRALDYLVGFTLSPVLWRKLP
                     GAKSAGRVQSVALRLVVDREREIELFRAQEYWSVAALMEADGQEFLARLVRYKGEKLD
                     RLSLGDEATALAAKAAVEAGRFAVESVETRPLTRNPPPPFTTSTLQQEAARKLGFAAS
                     HTMRVAQALYEDGSVDPDSGFAMEWETYADKARQLK"
     gene            1216..1911
                     /locus_tag="VF547_03320"
     CDS             1216..1911
                     /locus_tag="VF547_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8261886.1"
                     /translation="MIRAILAAALAMLAAPAQAQAPAAAAPPPPAPTGLEAQVLAGLN
                     AVRTDPAAYAATLREYRAHFEGNVVLLPGSDVGLRTREGVAAVDEAIAFLGRQTPLPP
                     FRYAPDLVEAAKALAADQALNGGTGHVTATVPDAKVRIKKHKGSGFMAEALSYGAGDA
                     GGIVRQLIVDDGAPSRPNRKLIFDKRYRMIGLACGPHPQVRSMCVLDMSTFVGLITAP
                     KQVRNPPPEIDIR"
     gene            complement(1964..>3210)
                     /locus_tag="VF547_03325"
     CDS             complement(1964..>3210)
                     /locus_tag="VF547_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261887.1"
                     /translation="AVLQQFHDAGRAIDQKRRELFWGGDDIGRPSGPLIWGAGEGYGY
                     FRVYGDGAIYWRRELGAHWVHGAIYAKYLRLGAETGFLGYPVTDESGAADGIGRFNHF
                     ERGSIYWHPSVGAFEVHGAILAKWRALGSEAFGYPITDESPAEGSGRFNHFRTFLPQG
                     DFADASIYWTEWTGAQAVYGAIRRRWAQLGWERGYLGYPVTDERAWTDPDSHLPGRVS
                     HFQRGVIAWQADNGAVTEFPERIVVHTGHFGVSSVGGSGEIVLSSAGYYKVHIHLHNS
                     GFVGLHCTVGIIVRFNGTKFLFVPPSAEMSVGGTISFDGRDEDWDEEGYLEDLRTKWS
                     EIRHGWSAETHIVAGLDAVSFFLLVMAPLVAGAIILGGSGKPPPETHCITGPMHTVKD
                     GNNNTVAEPEAVRCGLPGPPPP"
BASE COUNT          520 a         1112 c         1067 g          511 t
ORIGIN      
        1 cttcagttgc cgcgccttgt cggcataggt ctcccattcc atggcgaagc cggagtcggg
       61 atcgaccgag ccgtcctcgt agagcgcctg ggcgacgcgc atggtatggc tggcggcgaa
      121 gcccaatttg cgcgccgcct cctgctgcag ggtggaggtg gtgaagggcg gcggcgggtt
      181 gcgggtgagc ggccgggtct cgaccgattc gacggcgaag cggccggcct cgaccgccgc
      241 cttggccgcc aaggccgtgg cttcgtcgcc caggcttagc cgatcgagct tctcgccctt
      301 gtagcggacc agccgggcga ggaattcctg gccgtcggcc tccatcaggg cggcgacgct
      361 ccaatattcc tgggcccgga acagctcgat ctcgcgctcg cggtcgacga cgagacggag
      421 cgccaccgac tggacccggc cggccgactt agcgccgggc agcttgcgcc agagcaccgg
      481 cgacagagtg aatccgacga gatagtcgag cgccctccgg gcgcggtagg cgtcgatcag
      541 atcctcgtcg agcgcccggg gatgcttcat cgcctcggtc acggtcgcct tggtgatggc
      601 gttgaaggtg acccgctcga cgtcggccgg gagggccttg cgcttcctca gcacctcctg
      661 gacgtgccag ctgatcgcct ccccctcgcg gtcggggtcg gtggcgagga tcagcgaatc
      721 cgccttcttc gcctcgtcgg tgatcgcctt cagttgccgc gccttgtcgg cataggtctc
      781 ccattccatg gcgaagccgg agtcgggatc gaccgagccg tccttgggcg gcaggtcgcg
      841 gacatggccg taggaggcaa gcacgcggta gcccgcgccc agatatttct cgatggtttt
      901 cgccttggcg ggcgattcga cgacgacaag cttcatggga acccttcacg cgtacgcgta
      961 cagggtgggg aggccgggcg agtcccgtca agcggcgaaa ccggcccgcc gccgggcgcc
     1021 gcacgccgga aggccgccgg gccgggggcg ccgccggctc cgcgggagcg cggcggaccg
     1081 ttccccggcc gcgacgcggg gaacggcgcg ctcgccctgc aacttttcgc agagccgcgg
     1141 gcgccgggcg acgagaatga ttggaatgag tccggttccg ctgtagcctg acgggcaaaa
     1201 gccagggaag ccatgatgat acgcgcgatc ctcgccgccg ctctggcgat gctggcggcc
     1261 cccgcccagg cccaggcgcc ggccgccgcc gcaccgccgc cgccggcgcc gacaggtctc
     1321 gaggcccagg tgctcgccgg gctcaacgcg gtgcgcaccg atccggcggc ctatgccgcg
     1381 accctccggg agtatcgtgc ccatttcgag ggcaatgtgg ttcttctgcc cggcagcgac
     1441 gtcggccttc gcacccgcga aggggtcgcg gcggtcgacg aggccatcgc cttcctcggc
     1501 cggcagacgc cgctgccgcc tttccgctac gccccggacc tggtggaagc ggccaaggcg
     1561 ctggccgccg accaggcgct caacggcggc accggccacg tcaccgccac cgtgccggac
     1621 gccaaggtcc ggatcaagaa gcacaagggc agcggcttca tggccgaggc cctgtcctac
     1681 ggcgcgggcg acgcgggcgg gatcgtgcgc cagctgatcg tcgacgacgg cgctccgagc
     1741 cggcccaacc gaaagctgat cttcgacaag agataccgga tgatcggcct cgcctgcggc
     1801 ccccatcccc aggtccgctc gatgtgcgtc ctcgacatgt cgaccttcgt cggcctgatc
     1861 accgcgccca agcaggtgcg caacccgccg ccggagatcg acatccgtta gcctgtcggc
     1921 gcagctcgtc cggccggcgc gcgggatttt cgcggcggac cggctagggc gggggtgggc
     1981 ccgggagccc gcaacggacg gcttccggct cggcaacggt attgttgttc ccgtccttga
     2041 ccgtatgcat gggaccggtg atgcagtggg tttcgggggg cggcttgccg ctcccgccca
     2101 agatgatcgc ccccgcgacc agcggcgcca tcaccagcag gaagaagctc accgcgtcga
     2161 gaccggcaac gatgtgagtc tcggcgctcc agccatgccg gatttcgctc catttggtgc
     2221 gaaggtcctc caaataccct tcctcgtccc aatcctcatc tcgcccgtca aacgagatgg
     2281 tcccgccgac gctcatttcc gcggagggcg ggacgaacag gaacttggtg ccgttgaatc
     2341 tcacgatgat cccaacggtg caatggagac cgacgaaacc gctgttatga agatgaatgt
     2401 ggaccttgta ataaccggcg ctgctcaaga cgatctcgcc tgaaccgcca acggaggaca
     2461 ctccgaaatg cccggtatgc accacgatgc gttccgggaa ctcggtcacc gcgccgttgt
     2521 ccgcctgcca cgcaatgacc ccgcgctgga aatggctgac acggccgggg agatggctgt
     2581 ccggatcggt ccaggccctc tcgtccgtca ccgggtagcc cagatagccc cgctcccagc
     2641 ccagttgggc ccagcggcgg cgaatggcgc cgtagacggc ctgagcgccg gtccattcgg
     2701 tccaataaat cgatgcgtcg gcaaaatcgc cctggggaag gaacgttcgg aaatgattga
     2761 accgcccgct tccctcggcc gggctttcgt ccgtgatcgg atagccgaac gcttccgagc
     2821 caagcgcccg ccatttggcg aggatcgcgc catgaacctc gaaggcgccg accgaagggt
     2881 gccagtagat cgaacccctc tcgaaatgat tgaatcggcc gattccatcg gccgcgccgc
     2941 tctcgtccgt caccggatag cccaggaagc ccgtctcggc gccgagacga aggtatttgg
     3001 cgtagatggc gccgtgcacc caatgcgctc ccagctcgcg tcgccaatag atcgcgccat
     3061 cgccatagac gcggaaatac ccatagcctt cgccggcccc ccaaatgagc gggccggacg
     3121 gccgaccgat atcgtctccg ccccagaaca gctcacggcg cttttgatcg attgctcttc
     3181 cggcatcgtg gaactgctgc agcacggcgg
//
LOCUS       DASVQZ010000120         8081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_315177, whole genome shotgun sequence.
ACCESSION   DASVQZ010000120 DASVQZ010000000
VERSION     DASVQZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315177"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VF547_03330"
     CDS             complement(<1..1045)
                     /locus_tag="VF547_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX8261888.1"
                     /translation="MLLLLALLAGDLAIAQAPPTLAEAQREAAAAKRRSDLLERRARR
                     ATGEAARANAAAAALAARIEAAEADITGAEARIALIERLSAEQRARLAAGQGSLIRLT
                     AALQTMARRPPALALIQPGSLDEVVHVRALLASSLPAIRARTAGVRAELARGEALRRE
                     AAAAVVQLRSSRQDLQQRRLALARFEQGQRSRSATLMQSAVQESDRALAFGEEARNLA
                     ALAGTRAFQTRLARRLAELPGPAPHPDRRPADTPPPVRYLLPVAGRLVTGTGEISDSG
                     VHARGLTFDVAAGTTAVAPRAGRIAFAGPVRGYGAVVIIDHGGGWTSTVTDLAGLAVK
                     RGDRVSARQPLGRT"
     gene            complement(1197..1619)
                     /locus_tag="VF547_03335"
     CDS             complement(1197..1619)
                     /locus_tag="VF547_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX8261889.1"
                     /translation="MRLHIVARGRIGRGPEAELVERYLKRIAWPTRVTELPESGGRLP
                     APSEHSVTVLLDEKGEALGSIDLARRLERWRDEGRREARFLIGGADGFDDRDRASADL
                     LLSFGRATWPHLLARAMLAEQLWRATSILANHPYHREG"
     gene            complement(1797..2201)
                     /gene="rsfS"
                     /locus_tag="VF547_03340"
     CDS             complement(1797..2201)
                     /gene="rsfS"
                     /locus_tag="VF547_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX8261890.1"
                     /translation="MPANAPAPTTSGAHEKVEALHDLVLRSLDDDQAQDVVTIPLTGK
                     SNIADHMVIASGRSTRQVASMAQKLGERIKQELGRTVRVEGLPVADWVLIDADDVIVH
                     IFRPEVRSFYNLERMWAFEGDAGAAPAATAAR"
     gene            complement(2211..2852)
                     /locus_tag="VF547_03345"
     CDS             complement(2211..2852)
                     /locus_tag="VF547_03345"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX8261891.1"
                     /translation="MIRTGLLGGSFNPAHRGHRRVSAAAIRALGLDEMWWLVSPGNPL
                     KPAEGMAPLPARLASARRLARGLPIRATAIEAELGTRYTVDTLAALLPLYPERRFIWL
                     MGEDNLSQFHLWKHWRKLARTVPIAVVSRPGYDGAAHAARAMGWLRRFVQPADQARHW
                     TEWRPPALVLLRLPLDPTSATGIRAADPNWHQSEAYSFNPRALRDGVTRRLVT"
     gene            2918..3610
                     /locus_tag="VF547_03350"
     CDS             2918..3610
                     /locus_tag="VF547_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8261892.1"
                     /translation="MAADDEDRNMPDQDVREFIRETFGCSDEIAGYVFARGTVRAWPP
                     QSVIVREGEKAAATYLLTQGRAHALLYSLEGQVVLLFEYRPGDLFGALGELDPAPEEA
                     EIVAVEAARSFILKSRDLVALAESYGAIGLALARLLLRQLRRATSRMFERAAVSAIGR
                     VYKEILRLARASPDFVIRPAPVLSELAVRVSTTRETASRAVSALERRGIIRRDADSLT
                     VVAPQRLEELII"
     gene            complement(3563..5173)
                     /locus_tag="VF547_03355"
     CDS             complement(3563..5173)
                     /locus_tag="VF547_03355"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEX8261893.1"
                     /translation="MAPSLPLIVALARAGANDEAWRLLREGGYDRGDDPKALGVMARL
                     LKDQALRARGQERRRLYRESAVAYLRSAKARAATYPIINAASLSLLAGDGAGAATLAA
                     KILERIDAEPDEPETPYYRAATRAEALLLLDRHDEARAAFAEAMALAPQAWEDHASTL
                     RQFTLILEAQRRDASWLDAHRPPRSLHFGGHMSFDAKVIRREHLDEKIAAVLEEERVG
                     FGYGALAAGADIIIAEALLERGAEVHAVLPGGAEAFAAVSVDPFGKAWRKRFDAVLAK
                     AETVRPVRPLGARPGPAMIGLADEVAMGAALMNGRRLESSAVQLLVLDCAQEANGTSG
                     RSREIWAAGGWRQRILTAARETRPDPAAAESEEPAPLAVLAVGLAEGEIGERLAELHA
                     RLSRLPAPALPPYLTEDRLILAYRGLGEAAEAAIRMLGCGSGQEALRIGGQYGPAATF
                     RDPFSGTPRLAPAATAAAAGALASAPPGTACVTDDFAAALAAAGPRRPASEFIGELDA
                     PDGGPPVGLYALKSYDQLFQPLGGDDGQ"
     gene            complement(5226..5819)
                     /locus_tag="VF547_03360"
     CDS             complement(5226..5819)
                     /locus_tag="VF547_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261894.1"
                     /translation="MTNTVITTAAGVHTAYFYYLDYETGQPIISVYTKHDPDHPLGGV
                     RQVAQELADNHLGPRTDPTYGGDEFEDITRTWKGYFIVALEGASFSSPNPITFLCDSG
                     SVPQSLGNDGRHTFTPLGTFEIDSRGKSLSVTAYLYRAQSFIRPDDLDEGEREHYRIR
                     FSADGISAATVTYDDSGGTNMGPPPPPPFAPPASPES"
     gene            complement(5837..7528)
                     /locus_tag="VF547_03365"
     CDS             complement(5837..7528)
                     /locus_tag="VF547_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8261895.1"
                     /translation="MAETMPGAAGGYVAFISYSHKDAKVGRWLHRKLEGYRLPKRLAG
                     TEGEDGEVPERLTPIFRDRDNLPAAGDLNEKVRAALVVSRNLLVICSPNSAESPWVAK
                     EIATFRELHPDRPIFTAIVEGEPGQCFSPALLEGGVEPLAADLRHEGDGRRLGVLKLV
                     AGLAGVGLDALVQRDAARRVRRVTYVTGMAVAAMLAMALLTAFALNARAEAERHRAEA
                     EGLVEFMLTDVRRELRKVGRLDLMAVVNQRALSYYDAQKAGGAGAASAVRARADQTMG
                     EDLLTRGDTGSALRHFQQAYQTTSSLFERFPTDPKIIFVHAQSEYWLGRTHELRREWP
                     LAESRYTRFAALTDRLIATGPDNPDYMAQVAWAAVDLGNIQLNGSRNPEAAQRSYEKA
                     VTWFGRTAEARPRETAAQLALANAYGWLADSFFVRSLWQESVQARLRQYRIVERLYRG
                     DPKSLENGYRLALAQRGLGRSYAKAGDPARARPLTFEAHAWSRRLVKHDPHNADWLLF
                     SAFIGCDLYFGPLGLPPNLSKDDLRREIRGAATTLKAQGNPRASEFANCVGALGG"
     gene            complement(7645..>8081)
                     /locus_tag="VF547_03370"
     CDS             complement(7645..>8081)
                     /locus_tag="VF547_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261896.1"
                     /translation="ADAEAIEAIEKLTGARIRPVEGMAEAAGPAEAEPAGEEDERPRR
                     REREARPPRGRGRREEAPPRESRSRREEPAAEREPERDEPRREPEPPRETRRPEPRPR
                     REEPRGEPRRAAEPRRPAEPEAQEEEAGWNGPVPSFLEHKLG"
BASE COUNT         1143 a         2779 c         2922 g         1237 t
ORIGIN      
        1 cggtgcgccc cagcggctgg cgcgcgctca ccctgtcgcc gcgcttcacg gcgaggccgg
       61 caaggtcggt cacggtgctg gtccagccgc cgccatggtc gatgatcacc accgcgccat
      121 agccgcggac cgggccggcg aaggcgatcc ggccggcgcg cggggcgacc gccgtcgtcc
      181 ccgccgcgac gtcgaaggtg aggccgcggg cgtggactcc ggagtccgag atctcgccgg
      241 tgccggtgac gaggcggccg gcgaccggca gcaggtagcg gacgggaggc ggcgtgtcgg
      301 ccggccgccg gtcggggtgc ggcgccggcc cgggaagttc ggcgaggcgg cgggcgaggc
      361 gcgtctggaa ggcgcgggtg ccggccaagg cggcgagatt gcgcgcctcc tccccgaaag
      421 cgagggcgcg gtcggattcc tgcacggcgg actgcatcag ggtcgccgag cgggaccgct
      481 ggccctgctc gaaccgcgcc agcgccagcc ggcgctgctg caggtcctgc cggctcgagc
      541 gcagctggac caccgccgcg gcggcttccc ggcgaagcgc ctcgccccgc gccagctccg
      601 cccggacccc ggccgtgcgg gcgcggatgg cgggcaggga cgaggcgagc agggcgcgga
      661 cgtggacgac ctcgtcgagc gagccgggct ggatcagggc cagggccggc ggccggcgcg
      721 ccatcgtctg aagcgccgcg gtgaggcgga tgagcgagcc ctggccggcg gccaggcggg
      781 cgcgctgctc ggcgctcagc cgctcgatca gcgcgatccg ggcctcggcg ccggtgatgt
      841 ccgcctcggc ggcttcgatg cgggcggcga gcgcggcggc ggcggcgttg gcgcgagcgg
      901 cctcgccggt ggcgcgccgc gcgcggcgct cgagcagatc ggagcggcgc ttggcggcgg
      961 ccgcctcgcg ctgcgcctcg gccagggtcg gcggcgcctg cgcgatcgcc aggtcgccgg
     1021 cgagcagggc gaggaggagg agcaatattc ccctccctga aagggagggg ttaggggtgg
     1081 gtaggcgagc gtagcgagac tttctggcga ctcggagttt cggccgcctc cggcggaccc
     1141 acccccagcc cctcccttcc agggagggga gcttctccat ctctccgctc atcccctcat
     1201 ccttcgcggt gataggggtg gttggcaagg atcgaggtgg cgcgccagag ctgctcggcg
     1261 agcatcgccc gggcgagcag gtgcggccag gtggcccgtc cgaacgacag cagcaggtcc
     1321 gccgaggcgc ggtcgcggtc gtcgaacccg tcggccccgc cgatcaggaa gcgtgcctcc
     1381 cgccggccct cgtcgcgcca gcgctcgagc cgccgggcga ggtcgattga gccgagcgcc
     1441 tcgcctttct cgtcgagcag gacggtgacc gaatgttcgg acggagcggg gagcctgccg
     1501 ccgctctcgg gaagctcggt gacccgggtc ggccaggcga tgcgcttcag gtagcgctcg
     1561 accagctccg cctcggggcc tcggccgatc ctcccgcggg cgacgatgtg caggcgcatc
     1621 ctaggctgac cggggcggac atggagaggc catcccccct cctactccgt tcgggccgag
     1681 cttgtcgaag ccctccctgg cggcggcccc gtcaggagca agcggccttc ggcgggctca
     1741 ggccgaacga tttggccggg gggcagcggg cccgcttcgc cccctcgttt cgcgattcag
     1801 cgtgccgccg tcgccgccgg agccgcgccc gcatcgccct cgaacgccca catccgctcc
     1861 agattgtaga agcttcgaac ctccggccgg aagatgtgga cgatgacgtc gtcggcgtcg
     1921 atcagcaccc agtcggcgac cggcaggccc tcgacccgga cggtccggcc gagctcctgc
     1981 ttgatccgct cgcccagctt ctgagccatc gaggcgactt ggcgggtcga gcggcccgag
     2041 gcgatcacca tatggtcggc gatgttggac ttgcccgtca aagggatggt gacgacgtcc
     2101 tgggcctggt catcgtccag cgatcgaagc acgagatcgt gcagggcctc gaccttctca
     2161 tgcgctccgc ttgtcgtcgg agccggtgcg ttagcgggca aactgcctcc tcaggtcacg
     2221 agccgccgcg ttacgccgtc gcgcaaggcc cgggggttga acgaataagc ttccgattgg
     2281 tgccagttcg gatcggcggc acgaatgcct gtggcggagg tcggatcgag cggcagtctc
     2341 aacagcacga gcgccggcgg tctccactcc gtccagtgcc gagcctggtc cgcgggttgg
     2401 acgaagcgcc gcagccagcc catcgcgcga gccgcgtggg cggcgccatc atatcccgga
     2461 cgcgaaacga ccgcaatcgg aaccgtccgg gcgagcttgc gccagtgctt ccagaggtgg
     2521 aactgtgaca gattatcctc acccatcagc cagatgaagc ggcgctcggg atagagcggg
     2581 agcagcgcgg cgagcgtgtc gaccgtgtag cgtgtgccca gctcggcctc gatcgcggtg
     2641 gcccggatcg gcaggccgcg cgccagccgc cgggccgagg cgaggcgggc cggcagcggc
     2701 gccatgccct ccgccggctt gagcgggttg ccgggcgaga ccagccacca catctcgtcg
     2761 aggccgaggg cgcggatcgc cgccgcgctc acccggcggt ggccgcgatg ggcggggttg
     2821 aacgagccgc cgaggaggcc ggtgcggatc acgggcaagg cgatagcatg gccgcggcgc
     2881 ttgccaagcg cttccgcctc tgtacttctg tccggccgtg gccgccgacg acgaagaccg
     2941 gaacatgccc gaccaggacg ttcgcgaatt catccgcgag actttcggct gcagcgacga
     3001 gattgccggc tacgtcttcg cgcgcggcac ggtgcgcgcg tggccgccgc agtcggtcat
     3061 cgtccgcgag ggcgagaagg ccgccgccac ctatctgctc acccaggggc gggcgcacgc
     3121 cttgctgtac agcctcgaag ggcaagtcgt ccttctcttc gaatatcgcc ccggcgacct
     3181 gttcggagcg ctgggcgagc tcgatcccgc gccggaggag gccgagatcg tcgcggtcga
     3241 ggcggctcga agcttcatcc tcaagagccg cgacctggtc gctttggccg agagctatgg
     3301 ggcgatcggc cttgccttgg cgaggctgct gcttcgtcag ctgaggcggg cgacctcgcg
     3361 aatgttcgag cgggccgcgg tctcggcgat cggacgggtc tataaggaga tattgcggct
     3421 ggcccgagcc tcgccggatt tcgtcatccg cccggcgccc gtcctttccg agctggccgt
     3481 gcgcgtctcg accacgcgcg agacggcatc gagagcggtg agcgcgctgg aacggcgggg
     3541 gatcatccgc cgcgacgccg actcactgac cgtcgtcgcc ccccagcggc tggaagagct
     3601 gatcatatga tttgagcgcg tagagcccga ccggcggccc gccgtcgggc gcgtcgagct
     3661 cgcctatgaa ttcgctagcc gggcggcggg gtccggcggc ggcgagcgcg gccgcgaaat
     3721 cgtcggtgac gcaggccgtc ccgggcggcg ccgaggcgag cgcgcccgcg gcggcggcgg
     3781 tggcggcggg cgcgagccgg ggggtgccgg agaaggggtc tcggaaggtg gcggcggggc
     3841 cgtactggcc gccgatgcgc agcgcttcct ggccggaacc gcaaccgagc atccgaatgg
     3901 cggcttcggc ggcctcgccg agaccgcgat aggccaggat gaggcggtcc tcggtgagat
     3961 atggaggcag cgcgggagcg gggagccgcg agaggcgggc gtgcagttcg gccagccttt
     4021 cgccgatctc gccctcggcg agtccgaccg cgagcacggc gagcggcgcc ggctcctccg
     4081 attcggcggc ggcggggtcg ggccgggttt cgcgcgccgc ggtgaggatc cgctgccgcc
     4141 agccgcccgc cgcccagatc tcgcgcgagc ggccggacgt gccgttggcc tcctgcgcgc
     4201 agtcgagcac gagcaattgc accgccgagc tctccagccg ccgcccgttc atcagcgccg
     4261 cccccatcgc cacttcgtcg gccagcccta tcatcgccgg cccggggcgg gcgccaagcg
     4321 ggcgaacggg gcggacggtc tcggccttgg cgaggacggc gtcgaacctc ttgcgccagg
     4381 ccttgccgaa cggatcgacc gacacggcgg cgaacgcctc ggccccaccg ggaagcaccg
     4441 catgcacctc cgccccccgc tcgagcaacg cctcggcgat gatgatgtcg gcgccggcgg
     4501 cgagcgcgcc gtagccgaag ccgaccctct cctcctcgag gacggcggcg atcttctcgt
     4561 cgagatgctc gcgccggatg accttcgcgt cgaacgacat gtgcccgccg aaatgcagcg
     4621 agcgcggcgg ccgatgggcg tcgagccagg aggcgtcgcg gcgctgggct tcgaggatca
     4681 gggtgaattg acgaagcgtt gaggcgtggt cttcccaagc ctggggggcg agcgccatcg
     4741 cttcggcgaa ggcggctcgg gcttcgtcgt gacgatcgag gaggagaagg gcctcggcgc
     4801 gggtggcggc gcggtaatag ggagtttcgg gctcatcggg ctcagcgtca attcgctcga
     4861 ggattttggc cgcaagcgtc gccgcccccg ctccgtcacc agcgagaagg ctcaggctcg
     4921 cagcgttgat gatgggatag gtcgcggccc gagccttcgc agagcgcaaa taggcgacgg
     4981 cggattcgcg gtagagccgc cgccgctcct gcccccttgc tcgcaaggcc tggtccttga
     5041 gcaggcgtgc cataacgcca agcgctttcg gatcgtctcc gcgatcgtag cctccttcgc
     5101 gaagcaggcg ccaggcctca tcgttggctc cggcccgggc aagggccacg atcaggggca
     5161 gggacggagc catcgacaca agctaagcta tcatccgtgc tgaggggtag agtccagcac
     5221 gccgcttacg attccggcga agcaggcggc gcgaagggcg gcggtggcgg cgggcccatg
     5281 ttcgtcccgc cggagtcgtc ataagtgacg gtcgccgctg agatgccatc ggcgctgaac
     5341 cggattcgat aatgttcgcg ttcaccctcg tcgagatcgt cagggcggat gaagctctgc
     5401 gctcggtaaa ggtatgcggt cacgctgaga ctcttgccgc gggagtcgat ctcgaaagtg
     5461 ccgagagggg tgaatgtgtg acgtccatca ttgccgaggc tctgcggcac gctgccgctg
     5521 tcgcatagaa aagtaattgg attgggtgaa ctgaaggatg ccccttcgag cgccacgatg
     5581 aaatatcctt tccatgttct cgtgatatcc tcgaactcat cacctccgta ggtcggatcg
     5641 gtgcggggtc cgagatgatt gtcagcaagt tcctgggcta cctgccggac cccaccaagt
     5701 ggatgatctg gatcgtgctt cgtgtagacc gagataatgg gctgcccagt ctcataatcc
     5761 aggtaataga aataggcagt gtggacaccc gcagcggtcg tgatgacggt gtttgtcatt
     5821 gacttgctcc ttcaggtcag ccccccaacg ctcccacgca attggcgaac tcggaggcgc
     5881 gtggattccc ctgagccttg agtgtcgtgg cggcgcctcg gatttcgcgc cgaaggtcgt
     5941 ctttgctgag attgggcggc agccccagcg gcccgaaata caggtcgcag ccgatgaagg
     6001 cgctgaaaag cagccaatcg gcgttgtgcg ggtcgtgctt gactaggcgc cgggaccagg
     6061 catgcgcctc aaaggtgagc ggccgcgctc gcgcgggatc gccggccttg gcgtaggagc
     6121 ggccgaggcc gcgttgggcc aaggctagcc ggtatccatt ctcaaggctt ttcgggtcgc
     6181 cgcggtacag gcgctctacg atccgatatt gccgcaggcg cgcctgcacc gactcctgcc
     6241 acaggctgcg cacgaagaag ctgtcggcta gccagccata ggcattggcg agtgcaagct
     6301 gcgccgccgt ttcacgcgga cgcgcctcgg ccgttcgccc gaaccatgtc accgccttct
     6361 cgtaggagcg ctgcgcagcc tcggggttcc ggctaccgtt gagctggata ttgccgaggt
     6421 cgacggccgc ccaggcgacc tgggccatat agtcgggatt gtccgggccg gtcgcgatta
     6481 gccgatcagt cagggccgcg aagcgtgtgt agcggctttc ggcgagcggc cattcgcggc
     6541 gcagctcgtg cgtgcgtccg agccagtatt cactctgggc atgaacgaaa attatcttgg
     6601 gatcggtcgg aaagcgttcg aatagcgagc tcgtcgtctg ataggcctgt tggaagtgtc
     6661 gcagagcgga gcccgtatcg ccgcgagtca ggaggtcttc ccccattgtc tggtcggccc
     6721 gtgcccgaac tgccgaggcc gctccggctc cgccggcctt ctgcgcgtcg tagtaggaga
     6781 gggcacgctg attcaccacg gccatgaggt cgagacggcc caccttcctc aattctcggc
     6841 gcacgtcggt cagcatgaac tccaccagtc cttcggcttc agcacgatgg cgttcggcct
     6901 cggcgcgggc gttgagcgcg aaggccgtca acagggccat ggctagcatc gccgccaccg
     6961 ccatccccgt gacgtaggtc acgcggcgga cgcgccgtgc cgcgtcgcgc tggacgaggg
     7021 cgtcgaggcc gacgccggcg aggccggcga ccagcttgag gacgccgagg cgccttccgt
     7081 caccctcatg gcgcagatcc gcggccaagg gctccacgcc gccttcgagc agcgccggcg
     7141 agaagcactg ccccggctcg ccctcgacga tggccgtgaa gatcggccgg tccggatgga
     7201 gctcccggaa cgtcgcgatc tccttcgcca cccatggcga ttcggcgctg ttcggcgagc
     7261 agatgacgag caagtttctc gacaccacca gcgccgcccg cactttctcg ttcagatctc
     7321 ccgccgccgg cagattgtcg cggtcgcgga agatcggcgt cagccgctcg gggacctcgc
     7381 cgtcctcgcc ctccgtcccc gcgagccgct tcggcagccg gtacccctcc agcttgcggt
     7441 ggagccagcg gcccaccttg gcgtccttgt ggctgtagct gatgaacgcg acatagcctc
     7501 cggccgcgcc gggcatggtt tcggccatcg tcctccccca ctggcgcggg ccccacccgc
     7561 gcctgcccgc aaggtgacag agcgggggcg gatcgacaat atatcaaata gttggattcg
     7621 gacggccgtc cgtcccgggt cggctcagcc cagcttgtgc tccaggaagc tcggcaccgg
     7681 accgttccag ccggcctcct cctcctgcgc ttccggctcg gccgggcgcc gaggctcggc
     7741 ggctcggcgg ggctcgcctc tcggctcctc gcgccgcggg cgcggctccg gccgacgcgt
     7801 ctcgcgcggc ggctccggct cgcgcctcgg ctcgtcgcgc tccggctctc gctcggccgc
     7861 cggctcctcg cgccgggagc gggattcgcg cggcggcgcc tcctcgcgcc gtccccggcc
     7921 gcgcggcggg cgcgcctcgc gttcgcggcg gcggggccgc tcgtcctcct cgccggccgg
     7981 ctcggcctcc gccggcccgg ccgcctcggc catcccctcg accggccgga tccgcgcccc
     8041 ggtcaatttc tcgatcgcct cgatcgcctc ggcgtcggcg g
//
LOCUS       DASVQZ010000121        12248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_893557, whole genome shotgun sequence.
ACCESSION   DASVQZ010000121 DASVQZ010000000
VERSION     DASVQZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893557"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1362
                     /locus_tag="VF547_03375"
     CDS             52..1362
                     /locus_tag="VF547_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX8261897.1"
                     /translation="MNKLPALLAILIATISSEAPAAEAEPWAERWEVRAEFTQGSAAG
                     TMTLAGKDGRIEGSSEPLDEAQFFPLTVTGRPRADGAELELANRGDSVGRLNVAVRDG
                     MLTGTGTLYGMAVKVSATRPGGARRAPVTHDFVPRLYHLQYSGRVPPALRIAPGDRVR
                     TRTLDNEGRDSQRQWRGMPGNPLTGPFHVEGAMPGDTLVVHLERVDLNRDTAHMYSGT
                     LNERSVQGGYPQQAAPGWGRTWILDRKKGTARLEEPGGRLAALELPLRPMVGSIGVAP
                     PLNQALFAGDLGFHGGNMDYRRHTAGATIHFPVWRAGALLSLGDGHALQGDGEISGQG
                     LETSLDVTFRVELIKGKTLGQVWSEDSDYVMVSGIDNGLDASLQMATTGLARWLKDRY
                     RLNDSEVAALLSAAIEYDVAEIVDSRPHVVARIAKKTLLAIARP"
     gene            1454..1873
                     /locus_tag="VF547_03380"
     CDS             1454..1873
                     /locus_tag="VF547_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261898.1"
                     /translation="MRRGRLLPAAMLLLAPAAAAAEPVYFHKPNVARDAFEADLSECV
                     ELAGGVRVQRQAVPYTPNIYAAAAGAFLAGFMASKERRQMTKNVLRTCMTEKGYRRVE
                     ATDAVSDELKKLDKAGREARLHTLAAAPEPEGRMLPQ"
     gene            1870..2610
                     /locus_tag="VF547_03385"
     CDS             1870..2610
                     /locus_tag="VF547_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261899.1"
                     /translation="MRWIVVAAAALAAAAPAAAVTLKQVPAALNPAKAYILVEYKLLK
                     NPYGGGMPGSRKTMPLKSGLVFARYDPALGDIRGMGKARANPVAPGQQAVEGFRSKPI
                     AKGPGSRLFLIEVEPDIWVVQGFGNTSFSLGSYTFELAPGSVTDLGVVTAEPDWAEGQ
                     KRPSMGRLLGAAMAGPFAKSQDIAPMRASFRPRGPSDIPLPAGLPAERVKPAAFTPDA
                     KFGNYLGGLVNRIEGVNARLRAGGTTTP"
     gene            2657..2998
                     /locus_tag="VF547_03390"
     CDS             2657..2998
                     /locus_tag="VF547_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261900.1"
                     /translation="MRRRSSLISCALGAALADLAWLAAAGAARSMDSPPTDGRYELEA
                     REAFDVTLGRLIECGATEIWFEDLWTHSPGRGVLEASRRNRRAIDCFDRRSIGAGPAW
                     APAPPTPGPRR"
     gene            3010..3363
                     /locus_tag="VF547_03395"
     CDS             3010..3363
                     /locus_tag="VF547_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEX8261901.1"
                     /translation="MRRLLYLGLGFAAVALGTLGIVLPLLPTVPFMLLAAFFFARSSP
                     RLEAWIVEHPRFGPHVRAWRDRGAISRAGKRAALAAFAISAAIGLALLPFPWLLAPLA
                     AGLIGGGWIATRPEA"
     gene            3395..4000
                     /locus_tag="VF547_03400"
     CDS             3395..4000
                     /locus_tag="VF547_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEX8261902.1"
                     /translation="MAPRALFATLFYEGSVGDPAFLAELEAACRALAADDRAGVRWSK
                     EHGYRGYTSYASLNDLPERDPRFAELVRRLNRHVAAFAADCAFDLGGRRLKLDSLWVN
                     LLRPGGAHSGHIHPHSAVSGTLYVAVPPGSGALKLEDPRLPMMMAAPPRRGDAPEGLR
                     SFVYAEPAPGRLFLWESWLRHEVVPGTGRGERISISFNYRL"
     gene            complement(4144..5310)
                     /locus_tag="VF547_03405"
     CDS             complement(4144..5310)
                     /locus_tag="VF547_03405"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8261903.1"
                     /translation="MIGLKASALLLVTATTLWAVPAQARLQAAKIDSAARAAMQRTGA
                     RGLAMAVIEDGNPVFVRAWGHRNAAGDPLQTDTVMYGASLTKSVFAYTVMQLVEEGKL
                     DLDRPLAEYLPRPLPEYGNLDAYGNWGDLAGDERWRKLTARHLLTHSSGFSNFSWDEP
                     DRKLRFHFEPGSRYAYSGDGLILLQFVIEKGLGLDLGQEMKRRVFDRFGMASTSMTWR
                     PDFARNLADGWKKDGGVEPHDERSRVRAAGSMDTTIADFARFAAAFMRGEGLKRATRA
                     EMIRGQLPIRSASQFPSLAPEAAPEKRWKGLAAGLGVVAFAGPQGRGFFKGGHNDSTG
                     NMWVCLERGRRCVVLLANDVRAEAAFPRLVEAILGETGLPWRWEYGWLPEAGSP"
     gene            5524..6624
                     /gene="ychF"
                     /locus_tag="VF547_03410"
     CDS             5524..6624
                     /gene="ychF"
                     /locus_tag="VF547_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX8261904.1"
                     /translation="MGFRCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGR
                     VAVPDPRLDEIARIARSAQEIETQLEFVDIAGLVRGASKGEGLGNQFLGNIREVDAIV
                     HVLRCFEGGDVTHVEGRVDPVADAEVVETELMLADLESLEKRVPNLVKKAQQGDKEAR
                     IQASVLGQALDLLRDSKPARLTEPRDAEETVALERAQLLTAKPVLYVCNVDEGDAASG
                     NAHSARVFAKAAAEGAGAVVISAAIEAEIATMDPADRPEFLADLGLKETGLSQVIRAG
                     YDLLHLITFFTAGPKEARAWTVEKGSKAPQAAGVIHTDFERGFIRAETIAYEDFVRFG
                     GEAGAKDAGRMRSEGKDYVVQDGDVMLFRFNV"
     gene            6621..7025
                     /locus_tag="VF547_03415"
     CDS             6621..7025
                     /locus_tag="VF547_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HEX8261905.1"
                     /translation="MSLERRLLQGAVAAGCVVPLLAGGAGLIEGPGFLREVGSRVPAD
                     LDSHFRYLSGLLFAVGLAFASCIPAIERRTGRFRLLALLVFVGGLGRLAASLTTGIPG
                     GGHVFGLAMELVAVPILVLWQGRVAAQAMSSR"
     gene            complement(7004..7702)
                     /locus_tag="VF547_03420"
     CDS             complement(7004..7702)
                     /locus_tag="VF547_03420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8261906.1"
                     /translation="MKIGRRAVNAVRFVLEDLVPPALRDSPLFLPLMYLAWGRDARRF
                     IAFRDRIRTMGPEEYAGFYAAITPIQGECDLNRACLDRILAEAEGPAILDAGCGRGYL
                     AERVARANPDARVVGADLAPPSAEGLPNLSFVEGWMGRLPFADKSFDTVLCTHTLEHI
                     LDIDAAVADLRRIAARRLILVVPRERESRYPLNLHLHFFPYAHSFLNRIHAPDGRFAC
                     EILQRDIYYREDMA"
     gene            complement(7733..8425)
                     /locus_tag="VF547_03425"
     CDS             complement(7733..8425)
                     /locus_tag="VF547_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261907.1"
                     /translation="MRRLHLALAALAAALVLPASAPTLAADSLKVAPAALDPKLAYLL
                     VRLGKRDEKVWNLVSFYRYDPQAEDLRGRGRAKANPVPAGEDKAAVVGPKPFLAEDGN
                     IRTYLVAVTPGRWVIASSPTTSFSLGSYQFDAKAGEITDLGTLYMGAENGSSIWPRLA
                     QIRSAPDIEKRAYTVADALLVEPAGDAAAAPAGLSALPRRPAVFLRAPRFGNHSGQMI
                     NAVIPLESHGAP"
     gene            complement(8422..8862)
                     /locus_tag="VF547_03430"
     CDS             complement(8422..8862)
                     /locus_tag="VF547_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261908.1"
                     /translation="MRRAMRIGIIALLLAGAPAAAAEDEDDPDDFYFHKAGVSKETAL
                     ADWEECRDLASAVEPPRGPSVYTPNVAAAAAAGFAQGFIRGGQRRHMGEAAMRKCFQV
                     KGYGRYAMTKEEAKQLYSGGWKTMREKLAEKAAGPAPSQPALVP"
     gene            complement(8930..10435)
                     /gene="gatB"
                     /locus_tag="VF547_03435"
     CDS             complement(8930..10435)
                     /gene="gatB"
                     /locus_tag="VF547_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8261909.1"
                     /translation="MTDYRIHGATGEWEVVIGLEVHAQVTSAAKLFSGASTEFGAEPN
                     SQVSLVDAAMPGMLPVPNAECLRQAVRTGMALEAKINPWSRFDRKNYFYADLPQGYQI
                     SQLYHPIVGEGEIEILLDDKDETTAKRIGVERIHLEQDAGKLMHDQHPSFSYVDLNRS
                     GVALMEIVSKPDMRSPEEAGAYIRKLRAILRYVGSCDGNMEQGSMRADVNVSVRRPGG
                     PLGTRTETKNVNSVRFIMAAIEHEASRQVDVLEGGGSIVQETRLWDPDKAETRSLRSK
                     EEAHDYRYFPDPDLLPVELSDDFLEQCRASLPELPDAKRRRYEQALGLSPYNASVLTA
                     DVTTARWFEALLDGPGIGSAPAASAGEQDLARRAANWVTSDLFGALNRLGRTIEDSPV
                     SPAQAAELLRLTAEGTLSGTLAKQVFEIMLETGEDPARIVEERGLKQTSDTGAIDAAI
                     ADVLAANPDKVAEYRGGKDKLFGFFVGQVMKAMQGKANPGVVNDLLRKALS"
     gene            complement(10550..12031)
                     /gene="gatA"
                     /locus_tag="VF547_03440"
     CDS             complement(10550..12031)
                     /gene="gatA"
                     /locus_tag="VF547_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX8261910.1"
                     /translation="MTQLTDLGIAAIRDGVRDGDFSAREIAEAFNASVAKGRGLNAFT
                     VETPGHALAAADAADAARGSGDLKPLAGVPLGIKDLFATEGVATTAGSRILEGFRPRY
                     ESTVSANLFAAGAGMLGKLNMDEFAMGSSNETSAWGPVLSPWRRSDGGNAPLTPGGSS
                     GGSAAAVAARLAPGVTGTDTGGSIRQPAAFVGITGIKPTYGRCSRWGIVAFASSLDQA
                     GPMAHDVRDCAILLEAMAGFDPKDSTSLDLPVPNWEAALSSDVRGKRIGIPKEYRIDG
                     VPAEIDRVWERGIELMRDAGAEIVEVSLPHTRYALPTYYIIAPAEASSNLARYDGVRY
                     GLRVAPDNGNLTDMYEATRSEGFGPEVKRRIMIGTYVLSAGFYDAYFNRAQKVRALIA
                     RDFERVWERCDLILTPTAPSAAFALGDKSADPIAMYLNDVFAVPASLAGLPAISVPAG
                     LDDQGLPLGLQLVGRALDEQGVLNAALALEERAGFTARPEAWW"
     gene            complement(12147..>12248)
                     /locus_tag="VF547_03445"
     CDS             complement(12147..>12248)
                     /locus_tag="VF547_03445"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX8261911.1"
                     /translation="RDDVVTDGGKRDDVLANAPAAEHGFFAVPKVIE"
BASE COUNT         1816 a         4121 c         4411 g         1900 t
ORIGIN      
        1 ggaattttca gggaaatggc gcatgtcgct gattatctga aagcggaccg aatgaacaag
       61 ctccctgcac tgctcgctat cctgatcgcg acgatctcgt cggaggcgcc ggcggcggaa
      121 gcggagccct gggccgagcg ctgggaggtc agggcggaat tcacccaggg cagcgccgcc
      181 gggaccatga ccctcgcggg caaggacggc cggatcgagg gcagctccga gccgctcgac
      241 gaggcccagt tcttcccgct gacggtgacc ggacggccgc gggccgacgg cgccgagctc
      301 gagctcgcca accgcggcga ctccgtcgga cggctgaacg tcgccgtccg cgacgggatg
      361 ctcaccggga cgggcaccct ctacggaatg gcggtcaagg tcagcgccac ccgccccggc
      421 ggcgcgcggc gagcgccggt cacccacgac ttcgtgccga ggctctacca tctccaatat
      481 tccggacgcg tgccgccggc gcttcgaatc gcgcccggcg acagggtccg cacccgcacg
      541 ctcgacaatg aagggcggga ttcgcagcgg cagtggcgcg ggatgccggg caatccgctc
      601 accggcccgt tccacgtcga gggggcgatg cccggcgaca cgctcgtcgt ccatctcgag
      661 cgcgtcgacc tcaaccgcga caccgcgcat atgtacagcg ggacgctcaa cgagaggtcg
      721 gtgcagggcg gatatcccca gcaagcggcg cccggctggg gacggacctg gatcctcgac
      781 cggaagaagg gcacggcccg gctggaggag ccgggcgggc ggctcgccgc cctcgagctg
      841 ccgctgcgcc cgatggtcgg ctcgatcggg gtcgcgccgc cgctcaacca ggcgctgttc
      901 gccggcgacc tcggcttcca cggcggcaac atggattatc gccgccacac ggccggagcg
      961 acgatccact tcccggtctg gcgggcgggg gcgctgctct cgctgggtga cgggcacgcg
     1021 ctccagggcg acggcgagat cagcggccag gggctcgaga cgtcgctcga cgtcaccttc
     1081 cgggtggagc tgatcaaggg caagacgctc ggccaggtct ggtcggagga ttccgactat
     1141 gtgatggtgt ccgggatcga caacggcctc gacgcctcgc tgcagatggc gaccaccggt
     1201 ctcgcccgct ggctgaagga ccgataccgg cttaacgatt cggaagtggc ggcgctgctg
     1261 agcgccgcga tcgaatatga cgtcgccgag atcgtcgact cgcgtccgca tgtcgtcgcg
     1321 aggatcgcca agaagacgct cctggcgatc gcccggccct gaggggccgc gggcgccggg
     1381 ataagcccgg cggatcaggc ggttgcaggc gaaggcgcgc cgggctagct tcaccaccga
     1441 tcgggggagc gacatgagac ggggacgtct gctgccggcc gcaatgctgt tgctggcgcc
     1501 cgccgcggcg gcggcggagc cggtctattt ccacaagccg aacgtcgcgc gcgacgcgtt
     1561 cgaggccgac ctgtccgaat gcgtcgagct ggccggcggg gtccgggtcc agcgccaggc
     1621 cgtcccctac acgcccaata tctacgcggc cgcggccggc gccttcctgg cggggttcat
     1681 ggcctcgaag gaacggcgtc agatgaccaa gaacgtgctc cgcacctgca tgaccgaaaa
     1741 gggctatcgc cgggtcgagg cgaccgacgc ggtgtcggac gagctcaaga agctggacaa
     1801 ggccgggcgc gaggcgcggt tgcacacgct cgccgccgcg ccggagcccg agggaaggat
     1861 gttgccgcaa tgagatggat cgtcgtcgcg gccgccgcgc tcgccgccgc cgcgcccgcc
     1921 gcggcggtca cgctcaagca ggtgccggcg gcgctgaacc cggccaaggc ctatatcctc
     1981 gtcgaataca agcttctgaa gaacccctat gggggcggca tgcccggctc gcgcaagacg
     2041 atgccgctca agtccggact cgtcttcgcc cgctacgatc cggccctcgg cgacatcagg
     2101 ggcatgggca aggcccgcgc caatccggtc gcgcccggcc agcaggcggt ggagggcttc
     2161 cgctcgaagc cgatcgccaa gggaccggga tcgcggctgt tcctgatcga ggtcgaaccg
     2221 gacatctggg tggtccaggg gttcggcaac accagcttct cgctcggctc ctacacgttc
     2281 gagctcgccc cgggatcggt cacggacctc ggcgtcgtca ccgccgagcc cgactgggcg
     2341 gaaggccaga agcggcccag catgggccgg ctgctcgggg cggcgatggc ggggccgttc
     2401 gccaagtcgc aagacatcgc gccgatgcgg gcgagcttcc gcccgcgcgg cccgagcgac
     2461 atccccctcc ccgctggcct cccggcggag cgggtcaagc cggccgcgtt cactccggac
     2521 gccaaattcg gcaattatct cggcggcctg gtcaaccgga tcgagggcgt caacgcccgg
     2581 ctgcgcgcgg gcgggacgac gacgccctag cctcgccgcg tttgcggtca ctgccgccga
     2641 tccgcataag gcggggatgc gccggcgttc cagcctgatc tcatgcgccc tcggcgcggc
     2701 cctggcggac ctggcgtggc tggcggcggc cggcgccgcg cggagcatgg actcgccgcc
     2761 gacggacggc cgctacgagc ttgaggccag ggaggctttt gacgtcaccc tgggcaggct
     2821 gatcgaatgc ggggccaccg agatttggtt cgaggatctg tggacccatt cgcccggccg
     2881 gggggtgctg gaggcgagcc gacggaaccg gcgggcgatc gactgcttcg accggcgcag
     2941 catcggcgca ggcccggcct gggcgcccgc gccgccgacg cccgggccca ggcgctgagc
     3001 gcggatccgg tgcgccgcct cctctatctc ggcctcggct tcgcggcggt ggccctcgga
     3061 accctgggaa tagtgctgcc gctgctgccg acggtgccgt tcatgctgct cgcggccttc
     3121 ttcttcgcgc gcagctcgcc caggctggag gcctggatcg tcgagcatcc ccggttcggg
     3181 ccgcacgtca gggcctggcg ggatcgcggc gcgatcagcc gcgccggcaa gcgggcggcg
     3241 ctggcggcct tcgcgatcag cgcggcgatc ggccttgccc tccttccctt cccctggctg
     3301 ctggcgcctc tggcggcggg gctgatcggc ggcggctgga tcgccacccg cccggaggcg
     3361 tgaccggcac gcccgcttga gctaggcggg agcgatggct ccccgcgcgc tcttcgcaac
     3421 gctcttctac gaaggatcgg tcggcgatcc ggccttcctc gccgagctcg aggcggcgtg
     3481 ccgggccctg gcggcggacg atcgggcggg cgttcgctgg tcgaaggagc acggctaccg
     3541 cggctacacc tcctacgcct cgctgaacga cctgccggag cgcgacccgc gcttcgccga
     3601 gctggtccgc cgcctcaacc gccacgtcgc cgccttcgcc gcggactgcg cgttcgacct
     3661 cggcgggcgg cggctgaagc tcgacagcct gtgggtcaac ctcctcaggc cgggcggcgc
     3721 ccattccggg cacatccatc cgcacagcgc cgtctcggga acgctctacg tcgccgtgcc
     3781 gccgggctcc ggagcgctga agctggagga tccgcgcctg ccgatgatga tggccgcccc
     3841 gccgcgccgc ggcgacgcgc ccgagggtct gcgcagcttc gtctatgccg agccggcgcc
     3901 ggggcggctg ttcctgtggg agagctggct gcggcacgag gtggtgcccg ggaccggcag
     3961 gggcgagcgg atcagcatca gcttcaacta ccggctctag agcattgagg aatcaggtcg
     4021 aatccttctc tcccgttcgc cctgagcctg tcgaagggct ttccttgccg ttccataaga
     4081 gaaaaggaga gggcttcgac aagctcagcc cgaacgcaag agagcgcaat ccgaccgagc
     4141 gctctaggga cttccggcct cgggaagcca gccatattcc cagcgccagg gcaggcccgt
     4201 ctcgccgagg atggcctcga ccagccgcgg gaaggcggct tcggcacgca cgtcgttggc
     4261 gaggaggacg acgcagcggc ggccgcgctc gaggcagacc cacatattgc ccgtgctgtc
     4321 gttgtggccg cccttgaaga agccccgccc ctgagggccg gcgaaggcga ccacgccgag
     4381 ccccgcggcc aggcccttcc accgcttctc cggggcggcc tccggggcga ggctcgggaa
     4441 ctggcttgcg gagcggatcg gcagctggcc gcggatcatc tcggcccggg tcgcccgctt
     4501 caggccctcg ccgcgcatga acgccgcggc gaagcgggcg aagtcggcga tggtcgtgtc
     4561 catcgagccc gcggcgcgga cccggctgcg ctcgtcgtgc ggctcgacgc cgccgtcctt
     4621 cttccagccg tcggcgaggt tgcgggcgaa atcgggccgc cacgtcatgc tcgtgctcgc
     4681 cattccgaaa cggtcgaaca cgcggcgctt catctcctgg ccgaggtcga ggccgagacc
     4741 cttctcgatg acgaactgga ggaggatcag cccgtcgccc gaataagcgt agcggctgcc
     4801 gggctcgaaa tggaagcgga gcttccggtc gggctcgtcc caggagaagt tgctgaagcc
     4861 cgagctgtgg gtgaggagat ggcgcgcggt gagcttgcgc cagcgttcgt ccccggcgag
     4921 atcgccccaa ttgccgtaag cgtcgagatt gccatattcg ggaagcgggc ggggcagata
     4981 ttcggcgagt gggcggtcga gatccagctt cccctcctcg accagctgca tgacggtata
     5041 ggcgaagacc gacttggtca gcgaagcgcc gtacatgacg gtgtcggtct gaagcgggtc
     5101 gccggcggcg ttacgatggc cccaggcgcg gacgaagacg gggttgccgt cctcgatgac
     5161 cgccatcgcg aggccgcgcg cgccggtgcg ctgcatcgcg gcgcgagcgg cggaatcgat
     5221 cttcgcggcc tgaaggcggg cctgggctgg aaccgcccac agagtcgtcg ccgtcaccag
     5281 cagcaatgcc gaagccttca acccgatcat gctacaaatg taacaggcat ggacttggct
     5341 gtcaccccga acttgtttca cggtccagtt tcgagcatcg ggtccttcga tacgggcctt
     5401 cgccttcgct cagtccctac tcaggatgag cggctgggat tattttctcc gctcatcctg
     5461 agtagccgct gagcgaagtc gaagcggcgt atcgaaggac cctccagtcg gaccggagca
     5521 ttcatgggtt ttcgttgcgg aatcgtcggc ttgcccaatg tcggcaaatc gaccctgttc
     5581 aatgcgctga ccgagaccgc ggcggcgcag gcggcgaatt atcccttctg cacgatcgag
     5641 ccgaacgtgg gccgggtcgc ggtgccggat ccccggctgg acgagatcgc ccgaatcgcc
     5701 aggtcggccc aggagatcga gacccagctg gagttcgtcg acatcgccgg cctggtgcgc
     5761 ggcgcctcga agggcgaggg gctgggcaac cagttcctcg gcaacatccg cgaggtcgac
     5821 gccatcgtcc atgtgctcag atgcttcgag ggcggcgacg tcacccatgt cgagggtcgg
     5881 gtcgatccgg tggcggacgc ggaggtggtc gagaccgaac tgatgctggc cgatctcgag
     5941 agcctcgaga aacgggtccc caacctcgtc aagaaggccc agcagggcga caaggaggcg
     6001 cggatccagg cctcggtgct cggccaggcg ctcgacctgc ttcgcgattc gaagccggcg
     6061 cggctcaccg agcccaggga cgcggaggag acggtcgcgc tcgagcgcgc gcagctgctc
     6121 accgccaagc cggtcctcta cgtgtgcaac gtcgacgagg gcgacgccgc ctcgggcaac
     6181 gcccacagcg cgcgcgtgtt cgcgaaggcc gcggccgagg gcgccggggc cgtcgtcatc
     6241 tcggccgcga tcgaggcgga gatcgcgacc atggaccccg ccgatcggcc cgaattcctc
     6301 gccgacctcg gcctcaagga gaccgggctc agccaggtga tccgcgccgg ctacgacctc
     6361 ctccacctga tcaccttctt caccgccggc cccaaggagg ctcgggcctg gacggtcgag
     6421 aagggctcga aggcgccgca ggcggccgga gtcatccaca ccgacttcga gcgcgggttc
     6481 atccgcgccg agaccatcgc ctacgaggat ttcgtcaggt tcggcggcga ggcgggggcg
     6541 aaggacgcgg gcaggatgcg ttccgaaggc aaggactatg tggtccagga cggcgacgtg
     6601 atgctgttcc ggttcaacgt gtgagcctgg agcggcggct gctgcagggc gcggtggcgg
     6661 ccggctgcgt cgttccgctt ctcgccggcg gcgccggcct gatcgaaggg ccgggctttc
     6721 ttcgagaggt ggggagccgg gtgccggccg acctcgacag ccatttccgc tatttgtccg
     6781 gccttctctt cgccgtcggc ctcgccttcg cgagctgcat ccccgcgatc gagagaagga
     6841 cggggcgctt ccggctgctc gccttgctcg tcttcgtcgg cgggctgggc cggctcgccg
     6901 cttccctgac gacggggatt ccgggcggcg gccatgtctt cgggctcgcg atggagctgg
     6961 tcgcggtgcc gattctcgtc ctctggcagg gccgggtggc ggctcaggcc atgtcttcgc
     7021 gatagtagat gtcgcgctgc aggatctcgc aggcgaagcg gccgtccggc gcgtggattc
     7081 ggttgaggaa gctgtgcgcg taggggaaga agtggaggtg gaggttgagc ggatatctcg
     7141 actcccgctc ccgagggacg accaggatga ggcgccgggc cgcgatccgc cggagatcgg
     7201 cgacggccgc gtcgatgtcg aggatgtgct ccagagtgtg ggtgcacagg accgtgtcga
     7261 acgacttgtc ggcaaagggg agccgcccca tccagccctc gacgaagctc aaattcggta
     7321 gtccttccgc cgagggcgga gcgaggtcgg cgccgacgac cctggcatcg ggattggcgc
     7381 gcgcgacgcg ctcggcgaga tagccgcgcc cgcagccggc atcgaggatg gccggaccct
     7441 cggcttcggc gaggatccgg tcgaggcagg cccgattgag gtcgcactcg ccctggatcg
     7501 gagtgatcgc cgcgtagaag ccggcatatt cctcgggccc catggtccgg atccggtcgc
     7561 gaaaggcgat gaaccgccgc gcgtcccggc cccaggccag gtacatcagc ggaaggaaga
     7621 gcggcgaatc gcgaagggcc ggaggaacca ggtcctcgag cacgaatcgc acggcgttga
     7681 ccgcccttct gccgattttc atgccgagtc cgctggagtt gcgccggaag gctcaggggg
     7741 ctccatggct ctccagcgga atcacggcat tgatcatctg gccgctatgg ttgccgaagc
     7801 ggggcgcccg caggaagacg gccggccggc gcggcagggc ggagagcccg gccggagccg
     7861 ccgccgcgtc gccggccggc tcgaccagca gggcatcggc gaccgtatag gcccgcttct
     7921 cgatgtcggg ggcgcttcgg atctgcgcca gcctcggcca gatcgagctg ccgttctccg
     7981 cgcccatgta gagcgtcccg aggtcggtga tctcgcccgc cttggcgtcg aactggtagg
     8041 agccgaggct gaagctggtg gtcgggctcg acgcgatcac ccagcggccc ggagtcacgg
     8101 cgacgagata ggttcgaatg ttcccgtcct cggcgaggaa gggcttgggc ccgacgacgg
     8161 cggccttgtc ctcgcccgcc ggcaccggat tggccttggc ccggcccctg ccgcgaaggt
     8221 cctcggcctg gggatcgtag cgatagaagg agacgaggtt ccagaccttc tcgtcgcgct
     8281 tgccgaggcg gacgagaaga taggcgagct tggggtcgag cgcggcgggc gcgaccttga
     8341 gcgaatccgc ggccagggtg ggcgcgctgg cgggaaggac cagggcggcg gcgagcgccg
     8401 cgagagccag gtggaggcgc ctcatgggac gagcgccggc tgggaaggcg ccggccccgc
     8461 cgccttctcg gccagcttct cccgcatcgt cttccagcct cccgaataga gctgcttggc
     8521 ctcctccttg gtcatcgcat agcgaccgta gcccttgacc tggaagcatt tgcgcatcgc
     8581 cgcctcgccc atgtggcgcc tctggccgcc acggatgaaa ccctgggcga atccggcggc
     8641 ggcggccgcg gcgacgttgg gcgtgtagac ggaggggccg cgcggcggct cgaccgcgct
     8701 ggcgaggtcg cggcattcct cccagtcggc gagcgccgtc tccttggaga cgccggcctt
     8761 gtggaagtag aaatcgtccg gatcgtcctc gtcttcggcg gccgccgccg gtgccccggc
     8821 gagcagcagc gcgatgattc cgatccgcat tgcccgtcgc atccgccatg ccctcccgag
     8881 ccctgcgtga accgggatga tgcgccgctc gaccggcctc gacaagaggc taggagagcg
     8941 ccttcctgag caggtcgttg accacgcccg gattggcctt gccctgcatc gccttcatca
     9001 cctggccgac gaagaagccg aagagcttgt ccttgccgcc cctatattcg gcgaccttgt
     9061 cgggattggc ggcgagcacg tcggcgatcg cggcgtcgat cgcgccggtg tcgctggtct
     9121 gcttcaggcc cctctcctcg acgatcctcg cgggatcctc gccggtctcg agcatgatct
     9181 cgaacacctg cttggcgagg gtgcccgaca gcgtgccctc ggcggtgagg cggaggagct
     9241 cggcggcctg ggcgggggag acggggctgt cctcgatggt tcggccgagg cggttcaagg
     9301 cgccgaacag gtcggaagtg acccagttcg cggcgcgcct ggccagatcc tgctccccgg
     9361 cggaggccgc cggggcgctt ccgatgcccg gcccgtcgag cagggcctcg aaccagcgcg
     9421 ccgtcgtgac gtcggcggtg agcaccgagg cgttgtaggg ggagaggccg agcgcctgct
     9481 cgtagcggcg gcgcttggcg tcgggcagct cggggaggct ggcgcggcac tgctcgagga
     9541 aatcgtcgga cagctcgacc ggaagcaaat cgggatcggg gaagtagcgg taatcgtgcg
     9601 cttcctcctt ggagcggagc gagcgggtct cggccttatc cgggtcccac agccgagtct
     9661 cctggacgat gcttccgcca ccttcgagga cgtcgacctg gcggctcgcc tcatgctcga
     9721 tcgcggccat gatgaagcgg accgaattga cgttcttggt ctcggtccgg gtgccgagcg
     9781 ggccgccggg ccggcgcacg ctgacgttga cgtcggcgcg catcgagccc tgctccatat
     9841 tgccgtcgca gcttccgacg tagcgcagaa tcgctctcag cttccggatg taagcccctg
     9901 cttcctcggg acttctcata tcgggcttgg agacgatctc catcagggcg acgcccgagc
     9961 ggttgaggtc gacataggag aagctcgggt gctggtcgtg catcagcttg cccgcgtcct
    10021 gctcgagatg gatgcgctcg accccgatcc gcttggcggt ggtctcgtcc ttgtcgtcga
    10081 gcaggatctc gatctcgccc tcgccgacga tcgggtggta gagctggctg atctggtagc
    10141 cctgaggcag atcggcgtag aaataattct tgcggtcgaa gcgcgaccac ggattgatct
    10201 tcgcctccag agccatcccg gtgcgcaccg cctggcgaag gcattccgcg ttcggcacgg
    10261 gcagcattcc cggcatcgcc gcgtcgacca ggctgacctg gctgttgggc tcggccccga
    10321 actcggtcga ggcgcccgag aagagcttgg cggccgacgt gacctgggca tggacctcca
    10381 gcccgatgac cacctcccat tcgccggtgg cgccgtggat ccgatagtca gtcatgggcg
    10441 cgttccccat cgtcatgcca gcgtaggctg gcatccatgc cgacttcggc atcgggctcg
    10501 ggctcggcat ggaccccagc cttcgctggg gtgacgaatt gaagacgcat caccaccacg
    10561 cctccggtct cgccgtgaag ccggcgcgtt cctccagcgc cagcgccgcg ttgaggacgc
    10621 cctgctcgtc gagcgcccgg ccgacgagct ggaggccgag cgggaggccc tgatcgtcga
    10681 ggccggccgg cacggagata gcgggcaggc cggcgaggct ggcggggacg gcgaagacgt
    10741 cgttcaaata catggcgatc gggtcggcgc tcttgtcgcc gagcgcgaag gcggcggagg
    10801 gggcggtcgg cgtcaggatc aggtcgcacc gctcccagac gcgctcgaaa tcgcgggcga
    10861 tcagcgccct caccttctgg gcgcggttga aataggcgtc gtagaagccg gccgagagga
    10921 cgtaggtgcc gatcatgatc cggcgcttga cctcgggtcc gaaaccctct gaccgggtcg
    10981 cctcgtacat gtcggtgagg ttgccgttgt cgggggcgac ccggaggccg tagcggactc
    11041 cgtcgtagcg ggcgaggttg gacgaggcct cggccggggc gatgatgtag taggtcggaa
    11101 gcgcatatct ggtgtgcggc agcgagacct cgacgatctc ggcgccggcg tcgcgcatca
    11161 gctcgatgcc gcgctcccag acccggtcga tctcagccgg cacgccgtcg atccgatatt
    11221 ccttcgggat gccgatgcgc ttgccgcgga cgtcggagct gagcgccgcc tcccagttcg
    11281 gcaccggcag gtcgagcgag gtcgaatcct tcgggtcgaa gccggccatg gcctcgagca
    11341 ggatcgcgca gtcgcggacg tcgtgcgcca tcggcccggc ctggtcgagg ctcgaggcga
    11401 aggcgacgat cccccagcgc gagcagcggc cgtaggtcgg cttgatgccc gtgattccga
    11461 cgaaggcggc gggctggcgg atcgagccgc cggtgtcggt gccggtgact ccgggcgcga
    11521 gcctcgccgc caccgcggcc gccgatccgc ccgagcttcc gcccggggtg agcggggcat
    11581 tgccgccgtc gcttcggcgc cagggcgaga ggaccggccc ccaggcgctg gtttcgttcg
    11641 acgagcccat ggcgaactcg tccatgttca gcttgccgag catgcccgcg ccggcggcga
    11701 acagattggc gctcaccgtc gattcgtagc gcggacggaa gccttcgagg atccggctgc
    11761 cggcggtggt ggcgacgcct tcggtggcga acaggtcctt gattccgagc gggactccgg
    11821 cgagcggctt gaggtcgccc gagccgcggg cggcgtcggc agcatcggcg gccgccaggg
    11881 cgtggccggg agtctcgacg gtgaaggcgt tgagtcccct gcccttcgcg accgaggcgt
    11941 tgaacgcctc ggcgatctcg cgcgccgaga agtcgccgtc gcggactccg tcgcggatcg
    12001 cggcgatgcc gagatcggtg agctgcgtca tggacacccc cgcccgttcg ggctgagctc
    12061 gtcgacgccc tcgccttttt cggcggtgga gaaatgaagg gcttcgacag gctcagcccg
    12121 aacggtctgc ttgcatcggc tggccatcat tcgatcacct tgggcaccgc gaagaagccg
    12181 tgctcggccg ccggggcgtt ggcgaggacg tcgtcgcgct tgccgccgtc ggtgacgacg
    12241 tcgtcgcg
//
LOCUS       DASVQZ010000122         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_700199, whole genome shotgun sequence.
ACCESSION   DASVQZ010000122 DASVQZ010000000
VERSION     DASVQZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700199"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1828
                     /gene="ligD"
                     /locus_tag="VF547_03450"
     CDS             14..1828
                     /gene="ligD"
                     /locus_tag="VF547_03450"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX8261912.1"
                     /translation="MPPFREPQKATLVDHVPSGSGWIHEMKYDGYRCLLAIGGGKAKV
                     YTRSGLDWSDKFPEIVAAAAELEIGSALLDGEIVALDEKGNTGFSALQQAISEGGRGL
                     TLFLFDALELDGEDLTGLPTIERKNRLASAVGEGRPPVILYAEHIVGHGEKLFEAMCS
                     AGQEGIISKRADSPYRGGRTKSWLKIKCTYRQEFVILGWSESDKKGRGFRALLLGVHE
                     DGKLRFAGKVGTGFSNQVQHDLLERFERIAAAKPAAPVPRADARGAHWLEPKLVAEVA
                     FAEFTADGVVRHASFLGLRGDKKAAEVVEERPQPVARTAAPGDDVKISNPERILFPDA
                     GVTKGELVDYYRTIGPAMMEWAANRPLSLVRCPQGRSSKCFFQKHNTGSFGPHVHQIA
                     IEEKKGEIEDYVYVADVAGAIACTQMGTIEFHGWGSKVESVEKPDRLVFDIDPDEGLS
                     FDQVKRTARDLHRHLADMGLQSFPMTTGGKGLHVVVPLTPRAEWPEVKDFAQRFAIAL
                     ATAEPERFTANLAKVKRKGRIFLDYLRNQRGSTAILPYSARARERAPVAAPISWDELE
                     DLTSGAPFTVRDGEELLERASGRALQGWGEASQVLPDL"
     gene            complement(1868..>2105)
                     /locus_tag="VF547_03455"
     CDS             complement(1868..>2105)
                     /locus_tag="VF547_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261913.1"
                     /translation="YRAAGAHNALVAQVDTLDLRLLGARKPALYLAGVPVTGKQGRRH
                     NLSGVNTVVTVVVIAAAVVGGYYLARAIDDSGEE"
BASE COUNT          345 a          744 c          722 g          294 t
ORIGIN      
        1 gggaggagca agaatgcccc ccttccgcga gccgcagaag gcgacgctcg tcgatcacgt
       61 cccgtccggc tccggctgga tccacgagat gaaatatgac ggctatcgct gcctgctcgc
      121 gatcggcggc gggaaggcga aggtctatac gcgctcgggg ctcgactggt cggacaagtt
      181 tcccgagatc gtcgccgccg cggccgagct cgagatcggc tccgcgctgc tcgacggcga
      241 gatcgtcgcc ctggacgaga agggcaatac cggcttctcg gcgctccagc aggcgatcag
      301 cgagggcggc aggggcctca ccctcttcct gttcgacgcg ctcgagctcg acggcgagga
      361 cctgaccggg ctgcccacga tcgagcgcaa gaatcgcctc gcctcggcgg tcggcgaagg
      421 ccggcccccg gtcatcctct atgccgagca tatcgtcggc catggcgaga agctgttcga
      481 ggcgatgtgc tcggccggcc aggaggggat catctccaag cgcgccgact ccccctatcg
      541 cggcggccgg acgaagtcct ggctcaagat caaatgcacc taccgccagg aattcgtcat
      601 cctgggctgg tcggaaagcg acaagaaggg ccgcggcttc cgggcgctcc tgctcggagt
      661 ccatgaggac gggaagctgc gcttcgccgg caaggtcggc accggcttct ccaaccaggt
      721 ccagcacgat ctgctcgagc gcttcgagcg gatcgccgcg gccaagccgg ccgcgccggt
      781 gcccagagcc gatgcgcgcg gcgcgcactg gctggagccc aagctggtcg ccgaggtcgc
      841 cttcgccgag ttcaccgccg acggcgtggt ccgccacgcc agcttcctcg ggctgcgcgg
      901 cgacaagaag gcggccgagg tcgtcgagga gaggccgcag ccggtcgccc ggaccgccgc
      961 gccgggcgac gacgtcaaga tcagcaaccc ggagcgaatc ctcttccccg acgccggcgt
     1021 caccaagggc gagctggtcg actattaccg gacgatcggc ccggcgatga tggaatgggc
     1081 ggcgaaccgg ccgctcagcc tggtccgctg cccgcagggc cggtccagca aatgcttctt
     1141 ccagaagcac aataccggaa gcttcggacc ccatgtccac cagatcgcga tcgaggagaa
     1201 gaagggggag atcgaggatt acgtctatgt cgcggacgtc gccggggcga tcgcctgcac
     1261 ccagatgggc acgatcgagt tccacggctg gggctcgaag gtcgagtccg tcgagaagcc
     1321 ggaccggctc gtcttcgaca tcgatccgga cgaaggcctc agcttcgacc aggtgaagcg
     1381 gaccgcgcgc gacctccatc gccacctcgc cgacatgggc ctgcagagct tcccgatgac
     1441 caccggcggc aagggcctgc acgtcgtcgt gccgctcacg ccgcgggcgg aatggcccga
     1501 ggtcaaggat ttcgcccagc gcttcgcgat cgcgctggcc accgccgagc cggagcgctt
     1561 caccgccaac ctcgccaagg tgaagcgcaa gggccggatc ttcctcgact atctgcgcaa
     1621 ccagcgcggc tcgaccgcga tcctgcctta ctcggcgcgc gcccgcgagc gggcgccggt
     1681 ggccgctccg atcagctggg acgagctcga ggacctgacg tccggcgccc ccttcaccgt
     1741 ccgcgacggg gaggagctgc tcgagcgcgc gtccggccgg gcgctccagg gctggggcga
     1801 ggcgagccag gtcctgccgg acctctaggc cgccgcgccg cggacgggcg gcaggaccac
     1861 gccgagccta ttcctcgccc gaatcgtcga tcgcccgggc gaggtaatag ccgccgacca
     1921 ccgcggccgc gatgacgacg acggtcacca ccgtgttgac cccgctcaga ttgtgccgcc
     1981 ggccctgctt tccggtcacc gggactccgg ccaggtacag cgccggcttc ctggcgccga
     2041 gcagccgcag gtcgagcgtg tcgacttgcg cgacgagggc gttgtgcgcc cccgcggcgc
     2101 gatag
//
LOCUS       DASVQZ010000123         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_506623, whole genome shotgun sequence.
ACCESSION   DASVQZ010000123 DASVQZ010000000
VERSION     DASVQZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506623"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VF547_03460"
     CDS             <1..550
                     /locus_tag="VF547_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261914.1"
                     /translation="LGRDKVEALLPALGVRAAARNRPFLSTRETFALKFGDPMRLARW
                     KAADETGRRAILPLLARPAPVIREPARFDGRPVAIGSVEWFASPADLVRTLDWLRRSG
                     DGTALELLAINPGLGPELARDFAYFGYKGGSEPGVLNMSFLLRSREGRWLAVSATWND
                     ESAALDEPRFAALMSRLVALMK"
     gene            complement(554..1441)
                     /locus_tag="VF547_03465"
     CDS             complement(554..1441)
                     /locus_tag="VF547_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261915.1"
                     /translation="MRHPFIAATVAGLALAAVPALAGKPVTRPMTCPIGGGTFQFTTS
                     ASYSTYGERPDGKPYGSWTFPLALPECPDNGLVLYKEYTPEEVARLEPLVGSEAYQAL
                     RKEDTPYYRAYWLMKAMGVEPERYLLALLQAGWEAEGRPELRKRYLAELAEASAAVPA
                     RPADINWIGMEGRAINALRELGRFDEASARLAKVPLAGLDAAGDTPEAKSRRGWLNFF
                     TALKPAIERRDSAIEPLDMLPRSVAFGRCIDRAGTLGEADKAFCARESAGVDELRSAR
                     AKMDKEMKALQQSREASGR"
     gene            complement(1620..2018)
                     /gene="apaG"
                     /locus_tag="VF547_03470"
     CDS             complement(1620..2018)
                     /gene="apaG"
                     /locus_tag="VF547_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX8261916.1"
                     /translation="MKGLFPHSATTGDVTVRVSVSFLPEQSEPGRGRWFWAYHVRIEN
                     ASRTAVQLISREWTIADGRGARHQVQGEGVVGEQPVIEPGGAFDYVSGGPLPTPTGWM
                     EGHYHMVAADGRGFDAAIPRFPLVAPAVTS"
     gene            2093..3289
                     /locus_tag="VF547_03475"
     CDS             2093..3289
                     /locus_tag="VF547_03475"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX8261917.1"
                     /translation="MTRDWSPASWRACEARHQPRYDDPAALEAALKELAAYPALVPSA
                     EAQALKAELVEAQAGRAFLLQGGDCAESFAEFSPETIRGTALLLDSMARRLAAASGLR
                     IVRVGRIAGQFAKPRSRELETRDGLSLPMYRGDIVNGIAFDEDSRRPDPERMFRAYGQ
                     SAATLSHLRALGGPEPIYTSHEALLLGYEQALTRRDGAGWYGSSAHFLWVGDRTRFAG
                     SAHVEFLRGLTNPIGIKCGPALRPDDLLELLAILNPAREPGRITLVSRMGLDEVERAL
                     PPLLRAVAAEGQPALWACDPMHGNTIRAANGFKTRPVGRILGELTAFFALCRAEGVRG
                     GGVHVEMTGRDVTECTGGAEAVTERDLAARYHTHCDPRLNRGQALELAERVAAELARD
                     RGAAAA"
     gene            complement(3296..3895)
                     /locus_tag="VF547_03480"
     CDS             complement(3296..3895)
                     /locus_tag="VF547_03480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX8261918.1"
                     /translation="MCNLYSNKVAPDAIARVFEQLAIPLRFPEGPPNLEPREEIRITD
                     PAPIVRWNPGAGVAELVQRRWSWPGPGGKPVYNFRSDGREFANSERGGRCLIVADGFY
                     EFTAPDTPRPDKRKDRWRFEVEGRDWFCIAGLWRSVEAGEAFTMLTTEPGADVAPYHN
                     RQVAVLAHERWRGWLDGSGSARDLLGPCPAGTLAVAKAG"
     gene            3984..4059
                     /locus_tag="VF547_03485"
     tRNA            3984..4059
                     /locus_tag="VF547_03485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4018..4020,aa:Glu,seq:ctc)
     gene            4535..>4758
                     /locus_tag="VF547_03490"
     CDS             4535..>4758
                     /locus_tag="VF547_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261919.1"
                     /translation="MALMFSRGRQSPSAGHVHGRSQFRHGGAATGARSLTSASKARLF
                     PMALAGASLALTPAAGQVVPPATVITPSSP"
BASE COUNT          673 a         1698 c         1673 g          714 t
ORIGIN      
        1 gctcgggcgc gacaaggtgg aggcgcttct gccggcgctc ggggtccgcg ccgccgcgcg
       61 caaccggccc ttcctgtcga cccgcgagac cttcgccctc aagttcggcg atccgatgcg
      121 gctggcccgg tggaaggcgg ccgacgaaac cggccggcgc gcgattctgc cgctcctggc
      181 ccggccggcc ccggtgatcc gcgaacccgc gcggttcgac ggccggccgg tggcgatagg
      241 cagcgtcgaa tggttcgcct cgccggccga cctcgtgagg acgctcgact ggcttcggcg
      301 gagcggcgac ggcacggcgc tcgagctgct ggcgatcaat ccgggcctcg ggccggagct
      361 cgcccgcgac ttcgcctatt tcggctacaa gggcggctcc gagcccggag tgctcaacat
      421 gagcttcctg ctgcgcagtc gcgagggccg ctggctggcc gtctcggcca cctggaacga
      481 cgagtccgcg gcgctcgacg aaccgcgctt cgcggcgctg atgagccgcc tggtcgcgtt
      541 gatgaagtag atcctatcgc ccgctggcct cgcgggactg ctggagcgcc ttcatctcct
      601 tgtccatctt ggcgcgggcc gagcgcagct cgtcgactcc ggcggattcc cgggcgcaga
      661 acgccttgtc ggcctcgccc agcgtgcccg cccggtcgat gcagcgcccg aaggcgacgc
      721 ttcgcggaag catgtcgagc ggctcgatgg cggaatcgcg gcgctcgatc gccggcttga
      781 gggcggtgaa gaagttgagc cagccgcggc ggctcttggc ctccggcgtg tcgccggcgg
      841 cgtcgagccc ggcgagcggc accttggcga gccgcgccga cgcctcgtcg aagcggccga
      901 gctcgcgcag cgcgttgatc gcgcggccct ccatgccgat ccagttgatg tcggccggcc
      961 gcgccggcac cgccgccgaa gcctcggcca gctcggccag gtagcgcttg cgcagctcgg
     1021 gccggccctc cgcctcccag cccgcctgga gcagcgccaa caaatagcgc tccggctcca
     1081 cccccatcgc cttcatcagc caataggcgc ggtaataggg cgtgtcctcc ttgcgcagcg
     1141 cctgataggc ctcggagccg accaggggct cgagcctggc gacctcttcg ggcgtatatt
     1201 ccttgtagag gacgaggccg ttgtcgggac attcgggcag cgccagcgga aaggtccagc
     1261 tgccgtaggg cttgccgtcg gggcgctcgc cataggtgct gtaggaggcg ctggtggtga
     1321 actggaaggt gccgccgccg atcgggcagg tcatcggccg ggtcaccggc ttgccggcaa
     1381 gggcgggaac ggcggcgagg gccaggccgg cgacggtcgc ggcgatgaaa ggatgacgca
     1441 tttcgggcac tccggggaaa cagcccttta atgcttattt gcacggagtt gaaccgtagc
     1501 tgtccttcga tacgggcctt cgccaagctc agtccctact caggatgagc ggttgggacc
     1561 attctccccg ctcatcctga gtagccgctg agcgaagtcg aagcggcgta tcgaaggacc
     1621 tagctcgtca ccgccggagc gaccagcggg aagcggggga tggcggcgtc gaagccgcgg
     1681 ccgtcggcgg cgaccatgtg gtaatggccc tccatccagc cggtcggggt cggcagcggg
     1741 ccgcccgaga catagtcgaa cgcgccgccc ggctcgatca ccggctgctc gccgaccacg
     1801 ccttcgccct gcacctggtg gcgggcgccg cggccgtcgg cgatcgtcca ttcgcggctg
     1861 atcagctgga ccgcggtcct ggaggcgttc tcgatccgga catgataggc ccagaaccag
     1921 cggcctcggc cgggctcgga ctgctcgggc aggaagctca ccgagacccg gacggtgacg
     1981 tcgccggtcg tggcgctgtg ggggaaaagg cctttcacgc gcgcttcctc tacttatcgg
     2041 ccgcacgcgt caatcgcttg cgcggcccgg cccgatccgc gaaggagcgg cgatgacgag
     2101 ggactggagc cccgcgagct ggcgcgcctg cgaggcgcgc caccagccgc gctacgacga
     2161 tccggcggcg ctggaagcgg cgctgaagga gcttgccgcc tatcccgccc tggtgccgag
     2221 cgcggaggcc caggccctca aggcggagct ggtcgaggcg caggccggcc gcgccttcct
     2281 cctccagggc ggcgactgcg ccgagagctt cgccgaattc tcgccggaga cgatccgcgg
     2341 cacggcgctc ctgctcgatt cgatggcgcg ccggctcgcc gccgcgagcg ggctgcgcat
     2401 cgtccgggtc ggccggatcg ccggccagtt cgccaagccc cgctcgcgcg agctggaaac
     2461 ccgcgacggg ctcagcctgc ccatgtaccg cggcgacatc gtcaacggca tcgcgttcga
     2521 cgaggactcg cgccgccccg acccggagcg gatgttccgc gcctacggcc agtcggcggc
     2581 gaccctcagc catttgcggg cgctcggcgg gccggagccg atctacacca gccacgaggc
     2641 actgctgctc ggctacgagc aggccctgac ccgccgcgac ggagcgggct ggtacggcag
     2701 ctcggcccac ttcctgtggg tcggcgaccg cacccgcttc gccggctcgg cccatgtcga
     2761 gttcctgcgc ggactcacca atccgatcgg gatcaaatgc gggccggccc tgcgccccga
     2821 tgacctgctc gagctgctcg ccattctcaa cccggcgcgc gagccgggcc ggatcaccct
     2881 ggtcagccgg atgggcctcg acgaggtgga gcgcgcgctg ccgccgctgc tgcgcgccgt
     2941 cgccgccgag ggccagccgg cgctgtgggc gtgcgacccg atgcacggca acacgatcag
     3001 ggcggcgaac ggcttcaaga cccgcccggt cggccggatc ctgggcgagc tcacggcctt
     3061 cttcgccttg tgccgcgccg agggcgtgcg cggcggcgga gtccatgtcg agatgaccgg
     3121 gcgcgacgtg accgaatgca ccggcggcgc cgaggcggtg accgagcggg acctcgccgc
     3181 gcgctaccac acccattgcg acccccgcct caaccgcggc caggcgctcg agctggccga
     3241 gcgggtcgcc gccgagctgg cccgcgaccg cggggccgca gcggcctgac ccgcctcatc
     3301 cggctttcgc caccgccagg gtccccgccg ggcagggccc gagcagatcg cgcgccgagc
     3361 cgctgccgtc gagccagccg cgccagcgct cgtgcgcgag cacggccacc tgccggttgt
     3421 gatagggggc cacgtccgcc cccggctcgg tggtcagcat ggtgaaggct tcgccggcct
     3481 cgacggaccg ccacaggccg gcgatgcaga accagtcccg cccctccacc tcgaaccgcc
     3541 agcggtcctt gcgcttgtcc gggcgaggcg tgtcgggggc ggtgaactcg tagaagccgt
     3601 cggcgacgat caggcagcga ccgccccgct ccgaattggc gaactcgcgg ccgtcggagc
     3661 ggaaattgta gaccggcttg ccgcccggcc cgggccagct ccagcgccgc tggacgagct
     3721 ccgcgacccc ggcccccggg ttccagcgga cgatcggcgc cgggtcggtg atccggatct
     3781 cctcgcgcgg ctcgaggttg ggcggtccct ccggaaagcg cagcgggatg gccagctgct
     3841 cgaagacccg ggcgatcgcg tccggcgcga ccttgttgct gtagagattg cacatcgcgc
     3901 cgccgcctta gtgcgcccgc ggctccgccg ccagcttgac agcggcggcc cctcccccga
     3961 tatgcgccgc tcgcaagcgc aacggcccct tcgtctagcg gtccaggacg tcgccctctc
     4021 acggcgaaaa cacgggttcg agtcccgtag gggtcaccag cttctctcag aaaatcgttg
     4081 caagccgttg atactgggcg attccaggac ggaggaattc gtcagctgcg gcggctcgcc
     4141 cgagcccgaa gcgacagccg cgcgcgtcca gtcgggaacg ttctcgtttg tttgtgggtg
     4201 cttataccgg atatcgaagg tttgatgtaa acccttccgg gtcatgatcc tcgcatgggc
     4261 tcgggccgat gcgaggggtt cccgagccgg tacgactcgg actcgaaggc gccccgcgtg
     4321 gccaccggcc gtcccgggaa acgcctccga aacgatccgc aagcctttcg gaaggcgtcc
     4381 gaagcgggat gacattcctg gtgtatccag cgacttaacg ctatttgatt atagggaacc
     4441 tcaagacttg gctgtcctga tcggggacaa gcgtcgtttg ctatgggtcc gataagggcg
     4501 tacaagtacg cgcttaggcg gggggcttca aagcatggct ttaatgttct cgcgcgggcg
     4561 gcagtcgccg tccgccggcc atgtgcatgg gcgttcccaa ttccgacatg gcggggccgc
     4621 caccggagcc cgcagcctga cgtccgcttc gaaggcccgc ctgttcccga tggcgctcgc
     4681 cggggcgtcg ctggcgctga cccccgcggc ggggcaggtc gttccgccgg cgaccgtgat
     4741 cacgccgtcg agtcccca
//
LOCUS       DASVQZ010000124         6221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_797057, whole genome shotgun sequence.
ACCESSION   DASVQZ010000124 DASVQZ010000000
VERSION     DASVQZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797057"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF547_03495"
     CDS             <1..579
                     /locus_tag="VF547_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261920.1"
                     /translation="WQDFPHSALSHHGEACCEIARQWVVAMDFAQLNGGGLTSGPRWL
                     RQKYKWGPSPWPMHWCEAVKRKTIDCGAHAALALEAFRARGLTAFPAQLVQRYSEEAT
                     EQWRGKWEGEDVACHWLDAEHIYHEATALLTGEDEVKIWDGSAGSWVAPRQAGGYGSL
                     VAVRICAAGPFGGGDGLRWGERRLKPGVWVQV"
     gene            complement(678..1388)
                     /locus_tag="VF547_03500"
     CDS             complement(678..1388)
                     /locus_tag="VF547_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8261921.1"
                     /translation="MNDIPAHVVVVDDDEDVRSTVAEYLRRNGLSVSEADGGAALRAL
                     MAERPIDLALLDINMPGDDGLTLAREIRASGEAGIIMLTAKSDDVDRIVGLEIGADDY
                     ITKPFNPRELLARVKAVLRRASRGGEAPATMGREVQMGKCRLNLDTRKLFEADGTEVP
                     LTSMEFDLLKCFAEHPRRVLSRDQLLDMAHSKEMEPFDRSIDTRIARIRRKIERDPSK
                     PSCLKTVRAAGYCFTPEC"
     gene            complement(1385..3328)
                     /locus_tag="VF547_03505"
     CDS             complement(1385..3328)
                     /locus_tag="VF547_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8261922.1"
                     /translation="MESRPSRLTAEEEEAREDSRRRLELAVEAAKVGTWTFDLRTGKT
                     WYSARSKQMYGLPADTEMTTAVIRKSVHPDDWEEVSRPYLTGFIEEEKVEVEYRVICP
                     DGSTRWIYSLGSARRDEHGVAQMINGIHLDITDRKLAEQELEETRQQFELAVKGANLG
                     SWTIDARTGESWYSARSRELYGVDGELHLDSRTLKAYIHPDDWEMAVEAARQGFPTDT
                     ISLEHRVVWPSGDVRWVHSLGTVQRDSNGKAEVLHGIHLDITDRKLAEQELARSRDAL
                     HQSEKLAALGSLLAGVSHELNNPLAAIVGQAEMLEEDSQGTIFEARAKKISAAAARCA
                     RIVQTFLAMARQKEAQRSLIDLNELVASSLEITEYGLRTAGIAVRVTLGTLLPKVVGD
                     RDQLHQVLVNLIVNAQQAMEHGEAFDKTLTLRTSVSHSGAVLLDVSDTGPGVPDKFRG
                     RIFEPFFTTKPQGMGTGVGLSFSHGIVEAHGGTITLEPSRRGAHFRLSLPPAAESDLR
                     AVPIDAETAAGTATGGAALIVEDEADVAETLRELLEREGYGVTVATDGAAALMAIDRG
                     DYDLIISDLRMPGVSGPDLHARLAETRPHLVERMGFVTGDTLGSSMDDFLRNCGRPVL
                     EKPFTKVGVRCLIASIVKPKVQA"
     gene            3519..3662
                     /locus_tag="VF547_03510"
     CDS             3519..3662
                     /locus_tag="VF547_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261923.1"
                     /translation="MTATTYRKSRLQLVLLAAAALGSSSAVLSGAMTVLAMQQPVQVA
                     TLA"
     gene            3688..4599
                     /locus_tag="VF547_03515"
     CDS             3688..4599
                     /locus_tag="VF547_03515"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEX8261924.1"
                     /translation="MYHNFNFRDVLASSARAAWQVEDVLPQGAKLDFGRRFLPEALAR
                     TDSIAFLSGDEKRVLNQIRGHEYLSLFGLVEEFILPFVLDHARPQLNGDDHRVRALLT
                     FAGEEAKHIHLFRLFHERFTEGFGTACASIGPAEAIAAEVLRHDPLSVALVILQLEWM
                     TQSHYLDSIRDDRDLDPLFKSLLRHHWIEEAQHAKLDTLMVEALAEGRDEAGIQVAID
                     GYLEIGAFLDAGLAEQARLDIDAFERATGAALGAGERAELNAHLHQALRWTYLGSGMV
                     HPRFTATLAAISPAARDRVAAVAPAFA"
     gene            4621..5178
                     /locus_tag="VF547_03520"
     CDS             4621..5178
                     /locus_tag="VF547_03520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8261925.1"
                     /translation="MLDTTTRPPVNGIDLAALDETVAAVADDARLGQVAFRVRTDWKG
                     QTKSESTVDSYTLAGRVVPRSFTIVADEPHELLGTNAAPNPQELLMSAVNACMVVGYV
                     AQASVRGIALDSCRIETEGELDLRGFLGLDETVPAGYRRISYTVFLEGDGTREQYEDM
                     HQAVMATSPNYFNMAQPIQMVGRLA"
     gene            5594..6160
                     /locus_tag="VF547_03525"
     CDS             5594..6160
                     /locus_tag="VF547_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX8261926.1"
                     /translation="MKTTLYALASVAVLAAAATPAAAEGFRAEIHGGWDNARGDGQGE
                     SGIVYGIGAGYDVAIAPKVELGLDLSADLSSVDECEGSVILAGDTACIDAKRDLAAAI
                     RLGYKVSDRGTLYALAGYTNARFRFDYTTPAGVTTGDSRNLDGFRLGAGYQHGFGEKM
                     YGKVEYRYSNYEADVTRHQVLLGVGVNF"
BASE COUNT          958 a         2190 c         2080 g          993 t
ORIGIN      
        1 tggcaggatt tcccgcacag cgccctctcc catcacggcg aggcctgctg cgagatcgcg
       61 cggcaatggg tggtcgcgat ggatttcgcc cagctcaacg gcggcggcct gacgagcggc
      121 ccgcgctggc ttcgccagaa atacaaatgg ggtccgtcgc cctggccgat gcactggtgc
      181 gaggccgtga agcgcaagac gatcgactgc ggcgcccatg ccgccctggc cctggaggcg
      241 ttccgcgccc gcggcctgac cgcttttccg gcccagctgg tccagcgcta cagcgaggag
      301 gcgaccgagc aatggcgggg caagtgggaa ggcgaggacg tcgcctgcca ctggctcgac
      361 gccgagcata tctaccacga ggcgaccgcc ctcctcaccg gcgaggacga ggtgaagatc
      421 tgggacggca gcgccggcag ctgggtcgct ccgcgtcagg ccggcggcta tggcagcctg
      481 gtcgcggtcc gcatctgcgc cgccggcccg ttcggcggcg gcgacggcct gcgctggggc
      541 gagcgccggc tcaagcctgg ggtgtgggtg caggtctgac ccatcccgtc attgcgagga
      601 gcgcagcgac gaagcaatcc aaccgctccc agggccggca ctggattgct tcgctgcgct
      661 cgcaatgacg acggagctca gcactccggg gtgaagcaat agcccgcggc tcggaccgtc
      721 ttcaggcagg acggctttga cgggtcgcgc tcgatctttc gccggatgcg cgcgattcgg
      781 gtgtcgatcg agcggtcgaa cggctccatt tccttggaat gggccatgtc gagcagctgg
      841 tcgcggctga gcactcggcg cggatgctcg gcgaagcatt tgagcaggtc gaactccatc
      901 gacgtcagcg gcacctcggt gccgtccgcc tcgaacagct tgcgggtgtc caggttgagc
      961 cggcacttgc ccatctgcac ctcgcggccc atcgtcgccg gcgcctcgcc gccgcggctg
     1021 gcgcggcgaa gcacggcctt gacgcgcgcg agcagctcgc gggggttgaa cggcttggtg
     1081 atgtagtcgt ccgctccgat ctccagtccg acgatgcggt cgacgtcgtc gctcttggcg
     1141 gtgagcatga tgatgccggc ttcgcccgaa gcgcggatct cgcgcgccag ggtgaggccg
     1201 tcgtccccgg gcatgttgat gtcgagcagg gccagatcga tcgggcgctc cgccatcagc
     1261 gcgcgcagag cggcgccgcc gtcggcctcg ctcaccgaca ggccgttgcg ccgcagatat
     1321 tcggcgaccg tggagcgcac atcctcgtcg tcgtcgacga cgacgacatg ggctggaatg
     1381 tcgttcatgc ctgtaccttc ggcttgacga tcgaggcgat cagacagcgc accccgacct
     1441 tggtgaacgg cttttcgaga accgggcggc cgcaattgcg caggaaatcg tccatgctgg
     1501 agcccagggt gtcgccggtg acgaagccca tccgctcgac caggtgcggc cgggtctcgg
     1561 cgaggcgggc gtggaggtcg gggccgctga cgccgggcat gcgcaggtcc gagatgatga
     1621 gatcgtagtc gccgcggtcg atcgccatca gcgcggcggc gccgtcggtg gcgacggtga
     1681 ctccatagcc ttcgcgctcg agcagctcgc gcagggtctc ggcgacgtcg gcctcgtcct
     1741 cgacgatcag cgcggcgccg ccggtggcgg tgcccgcggc ggtctcggcg tcgatcggca
     1801 ccgcccgaag gtcgctctcc gcggcgggcg ggagcgagag ccggaaatgg gcgccgcgcc
     1861 ggctcggctc gagcgtgatc gtgccgccat gggcctcgac gatgccgtgc gagaaggaga
     1921 ggccgacgcc ggtccccatc ccctgcggct tggtcgtgaa gaagggctcg aagatccggc
     1981 ctcggaactt gtccggcacg ccgggtccgg tgtcgctgac gtcgagcagg accgcgccgc
     2041 tgtggctgac cgaggtgcga agcgtcagag tcttgtcgaa ggcctcgcca tgctccatcg
     2101 cctgctgggc gttgacgatc aggttgacca gcacctggtg gagctggtcg cggtcgccga
     2161 ccaccttggg aagcagcgtg cccagggtca cccgaacggc gatgccggcg gtgcgcagcc
     2221 cgtattcggt gatctcgagc gacgaggcga cgagctcgtt gaggtcgatg aggctgcgct
     2281 gggcctcctt ctggcgggcc atggcgagga aggtctggac gatccgggcg cagcgcgcgg
     2341 cggcggcgct gatcttcttg gcccgcgcct cgaagatggt gccctggctg tcctcctcca
     2401 gcatctcggc ctggccgacg atcgcggcga gcggattgtt gagctcgtgg ctgactccgg
     2461 cgaggagcga gcccagcgcg gccagcttct ccgactggtg gagcgcgtcg cgcgagcggg
     2521 cgagctcctg ctccgcgagc ttgcggtcgg tgatgtccag atggatgccg tggagcacct
     2581 ccgccttgcc gttcgaatcg cgctggacgg tgcccagcga atggacccag cgcacgtcgc
     2641 cgctcggcca gacgacccga tgctcgagcg agatggtgtc ggttgggaac ccctggcggg
     2701 cggcctcgac cgccatctcc cagtcgtcgg gatggatata ggccttgagc gtgcgcgaat
     2761 cgaggtggag ctcgccatcg actccgtaga gctcgcgcga gcgcgccgaa taccagctct
     2821 cgccggtcct ggcgtcgatc gtccagctgc ccaaattggc gcccttgacc gccagctcga
     2881 actgctggcg ggtctcctca agctcctgct ccgccagctt gcggtcggtg atgtcgaggt
     2941 ggatgccgtt gatcatctgg gcgacgccat gctcgtcgcg ccgggcggag ccgagcgagt
     3001 agatccagcg cgtcgagccg tcggggcaga tcacccgata ctcgacctcg accttctcct
     3061 cctcgatgaa gccggtgaga tagggccggg agacttcctc ccaatcgtcg ggatggaccg
     3121 acttgcgaat caccgcggtg gtcatttcgg tgtccgcggg aagcccgtac atctgcttcg
     3181 agcgcgccga ataccaggtc ttgccggtgc gcagatcgaa ggtccaggtg ccgaccttgg
     3241 cggcctcgac ggcaagctcg aggcggcgcc gcgaatcctc gcgcgcctcc tcttcctccg
     3301 cggtgagacg cgacggtctc gattccatcc cctcaggcaa ggccgtttcc ccggaacagc
     3361 gagcagtgcc tccccatagc atcttccgcg gcggggcaaa cggtcttctg taacaattga
     3421 cacaaatttc cgcttcgcgc ggcatcggcc cgacatgggc ggagcgcatt aagccggggc
     3481 cgaggcgatg aatgcccggc agatccagga gacagaagat gactgccacc acctaccgca
     3541 agagccgcct gcagctcgtg ctgctcgccg cggccgcgct ggggtcgagc tccgccgttc
     3601 tgagcggcgc gatgaccgtc ctcgcgatgc aacagcccgt ccaggtcgcg acgctcgcct
     3661 gagcggcgcg gcttggaagg gaagacgatg taccataatt tcaacttcag ggacgtcctc
     3721 gccagctcgg cccgcgcggc ctggcaggtc gaggacgttc ttccccaagg cgccaagctc
     3781 gacttcggcc gccgcttcct gcccgaagcc ctcgcgcgca ccgattcgat cgcctttctg
     3841 agcggcgacg agaagcgcgt cctcaaccag attcgcggcc acgaatatct cagcctcttc
     3901 gggctggtgg aggagttcat cctcccgttc gtgctcgacc acgcccgtcc gcagctgaac
     3961 ggcgacgacc atcgggtccg cgccctgctc accttcgccg gcgaggaggc caagcacatc
     4021 catctcttcc ggctgttcca cgagcgcttc accgaagggt tcggaacggc ctgcgcctcg
     4081 atcggcccgg ccgaggcgat cgccgccgag gtgctccggc acgatccgct gtcggtcgcg
     4141 ctcgtcatcc tccagctcga atggatgacc cagagccact atctcgacag catccgcgac
     4201 gaccgcgacc tcgaccccct gttcaagagc ctgctcaggc atcactggat cgaggaggcg
     4261 cagcacgcca agctcgacac gctgatggtc gaggccctgg ccgaggggcg cgacgaggcc
     4321 gggatccagg tcgcgatcga cggctatctc gagatcggcg ccttcctcga cgcgggcctg
     4381 gccgagcagg cccggctcga catcgacgcc ttcgagcgcg ccaccggcgc cgcgctcggc
     4441 gccggggagc gggccgagct gaacgcgcat ctgcatcagg cgctccgctg gacctatctc
     4501 ggctcgggca tggtccaccc gcgcttcacc gccaccctcg ccgccatttc acccgccgcc
     4561 cgcgaccgcg tcgccgccgt cgcgccggcc ttcgcctgat cgcccccaag gagattttca
     4621 atgctcgaca ccaccacccg tccgccggtc aacggcatcg acctcgccgc cctcgacgag
     4681 acggtcgcag ccgttgccga cgacgcccgg ctgggccagg tcgcgttccg ggtccgcacc
     4741 gactggaaag gccagaccaa gtcggaatcg acggtcgatt cctacaccct cgccggccgg
     4801 gtcgtgccgc gcagcttcac catcgtcgcc gacgagccgc acgagctgct cggcaccaac
     4861 gcggcgccca acccgcagga gctgctgatg tcggcggtca acgcctgcat ggtggtcggc
     4921 tacgtcgccc aggcctcggt ccgcggcatc gcgctcgaca gctgccggat cgaaaccgaa
     4981 ggcgagctcg acctgcgcgg cttcctcggc ctcgacgaga ccgtgcccgc cggataccgc
     5041 cggatcagct acaccgtctt cctcgaaggc gacggcaccc gcgagcaata tgaggacatg
     5101 caccaggcgg tgatggccac ctcgcccaat tacttcaaca tggcgcagcc gatccagatg
     5161 gtcggccgcc tcgcctgatc ccttccccga cgcccctcgg gaaaactcgg ccccggcgca
     5221 ccccgcgccg gggccttttt gtccctgctc cccggcgcga agcacggccg gagccgcggc
     5281 tgacacaatt gaaaccaatt tcaacttggc gcgccatcgg gctgcaacag cgggcctcca
     5341 gaagggggtc accggccggg gaggctgggg ctgacaggag acggtttgtg tacatgtacc
     5401 gtgaaacgcc gttcaggcgt ctcctgctcg gctccctcct cctcggctgc ggcgtcagct
     5461 tcctcgacgg gatgctggcg tccctcaccc tcggcatggc ctagcttccg gagcctccgg
     5521 cgacctggcc gaacaagaac gaacctcagt gaaatcccgc ccggccgttg ccggtgcgga
     5581 cagggagtat gaaatgaaga ccactttgta tgctttggcc tccgtggccg tcctcgccgc
     5641 cgccgccacc cccgccgccg cggaaggctt ccgagccgag atccacggcg gctgggacaa
     5701 tgcccgcggc gacggccagg gcgagagcgg catcgtctac ggcatcggcg ccggctacga
     5761 cgtcgccatc gccccgaagg tcgagctcgg cctcgacctc agcgccgacc tcagctcggt
     5821 cgacgagtgc gagggcagcg tcatcctcgc cggcgacacg gcctgcatcg acgccaagcg
     5881 cgacctcgcc gccgcgatcc gcctcggcta caaggtttcc gaccgcggca cgctctacgc
     5941 gctggccgga tacaccaacg cccgcttccg cttcgactac acgaccccgg cgggcgtgac
     6001 gacgggcgac agccgcaacc tcgacggctt ccgcctcggc gccggctacc agcacggctt
     6061 cggcgagaag atgtatggca aggtcgagta ccgctactcg aactacgagg ctgacgtgac
     6121 ccgccaccag gtgctgctcg gcgtcggagt caatttctaa gtctccctcc gcccctcggg
     6181 agacacgggc tccggcggtt tcatcccgac cgccggagcc c
//
LOCUS       DASVQZ010000125         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_797102, whole genome shotgun sequence.
ACCESSION   DASVQZ010000125 DASVQZ010000000
VERSION     DASVQZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797102"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VF547_03530"
     CDS             complement(<1..1257)
                     /locus_tag="VF547_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261927.1"
                     /translation="MHKRPPRALFELLPQHLRNRDAEVGRPLEALTGLLAEELRIVEQ
                     DIDQLYDNWFIETCEPWVVPYIAALVGARGLRDIGGGEAGLRSFIANTLGYRQAKGTA
                     AALEQMARDVTGWPIVAVEFFQRLVWSQNVNHVRPEAPGIASVRDAEAARAAHGPFET
                     ACHLAGAGRAASWAGRYNLPNIGLFVWRLDAYPLGFLSNEPNGYLGGPQPRASQFGPG
                     LLHFDPLGTDRPLHNRPKPDLSIAARVDERVVPAPLDRRHLHRDLNALRGGVAGAGRW
                     FDDLPVVRIRLGGATLPAEKLFCCNLEARPDGGGGFVWERPADAGSVLFDPELGRLSL
                     HPDDEGKPVETVHAYAAPFDIGGGPYDRTASVEEWRGDFFPAGQAAPWRIGVSSRSQD
                     KTDDPDQAGPVVETLVAAIQRWNAEAG"
     gene            complement(1250..>2530)
                     /locus_tag="VF547_03535"
     CDS             complement(1250..>2530)
                     /locus_tag="VF547_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX8261928.1"
                     /translation="DIERGRAVSISGERSDAETITDSETLTVADVVHIGGHTRLLLEA
                     GPDYSYIRSSVRVNANLALATHGESFADTLGSGDASKANQVFTLARKPLTFVSAATPE
                     GRASTLEVRVDGILWHEVASLYDAGPYDRAYEVRIDDDGSTRVRFGDGERGHRLPTGT
                     LNVTAEYRAGIGAVGEVADEAIILLKTRPLGVRAVVNPSPASGSAEPETLGEARVRAP
                     QSVRTLGRIVSLTDYQDFALAFAGIGKARADILWSGQEQVVHLTVAPATDSLMDEEAT
                     VLGSLRDAIDLVRDGTDSVLIAPYLRRYFQLSARLYKHSDYLADDVAAAAREVLVAGF
                     GYSVRDLARPVSAAEAVALLQAVPGVIGVDLDSLALIDGNDPEAEGPGTLATVLPAYP
                     ARLLSAGEGGGTAPAELLTILESAIDIQVEEANA"
BASE COUNT          355 a          879 c          940 g          356 t
ORIGIN      
        1 ccccgcttcg gcgttccagc gctggatcgc ggcgacgagg gtctcgacga ccggccccgc
       61 ctggtcggga tcgtcggtct tgtcctgcga gcggctcgaa accccgatcc gccagggcgc
      121 ggcctggccc gccgggaaga agtcgccgcg ccactcctcg accgaggccg tccggtcata
      181 agggccgccg ccgatgtcga acggcgcggc ataggcatgg accgtctcga ccggcttgcc
      241 ttcgtcgtcc gggtggagcg agagccggcc gagctcgggg tcgaacagga cggagccggc
      301 gtcggccggg cgctcccaga cgaaccctcc gccgccgtcc gggcgcgcct ccagattgca
      361 gcagaacagc ttctccgccg gcagggtcgc gccgccgagg cggatccgga cgaccggaag
      421 gtcgtcgaac cagcgtccgg cgcccgccac gccgccccgc agcgcgttga gatcgcggtg
      481 gaggtgccga cggtcgagcg gcgccgggac cacccgctcg tcgaccctgg ccgcgatcga
      541 caggtccggc ttgggccggt tgtggagcgg gcggtcggtg cccaggggat cgaaatggag
      601 caggcccggg ccgaactggg acgcgcgcgg ctgcggcccg cccagatagc cgttcggctc
      661 gttgctgagg aagccgagcg gataggcgtc gaggcgccag acgaacaggc cgatgttcgg
      721 cagattgtag cgccccgccc agcttgccgc ccggccggct ccggcgaggt ggcaggcggt
      781 ctcgaacggc ccgtgggcgg cgcgggcggc ctcggcgtcg cggaccgagg cgattccggg
      841 cgcttcggga cggacgtggt tgacgttctg gctccacacc agccgctgga agaattcgac
      901 cgcgacgatc ggccagccgg tgacgtcgcg cgccatctgc tccagcgcgg ccgcggtgcc
      961 cttggcctgg cgatagccca gggtgttggc gatgaagctc ctgaggccgg cttcgccgcc
     1021 gccgatgtcg cgcaggccgc gcgcgccgac cagggccgcg atatagggga cgacccaggg
     1081 ctcgcaggtc tcgatgaacc aattgtcgta gagctggtcg atgtcctgct cgacgatccg
     1141 cagctcctcg gcgagcaggc cggtcagcgc ctcgagcggg cggccgacct cggcgtcgcg
     1201 gttgcgcaga tgctgaggga gcagctcgaa cagggctcgg ggcggccgct tatgcattgg
     1261 cctcctccac ctggatgtcg atcgctgatt cgaggatcgt cagtagttcg gccggagcgg
     1321 tgccgccgcc ctccccggcc gacagaagcc gcgcgggata ggccggaagc acggtcgcga
     1381 gcgtcccggg gccctccgcc tcgggatcgt tgccgtcgat cagggcgagg ctgtcgagat
     1441 cgaccccgat caccccgggc acggcctgga gcagggccac cgcctcggcc gcgctgaccg
     1501 gcctggcgag gtcccgcacg gaatagccga agcccgcgac cagcacctcg cgcgccgccg
     1561 cggcgacgtc gtcggcgaga tagtcggaat gcttgtagag gcgcgccgac aattggaaat
     1621 agcggcgcag gtagggggcg atcaggacgc tgtcggtacc gtcgcggacg agatcgatcg
     1681 cgtcgcgcag gctgccgagc accgtcgcct cctcgtccat caggctgtcg gtcgcgggcg
     1741 ccaccgtcag atggacgacc tgctcctggc ccgaccagag gatatcggcg cgcgccttgc
     1801 cgatgccggc gaaggccagg gcgaaatcct gatagtcggt gagagacacg atccggccga
     1861 gcgtgcgcac gctctggggg gcccgcaccc tcgcctcgcc gagagtctcg ggctcggccg
     1921 agccgctcgc cggggaggga ttgaccacgg cccggacgcc gagcggccga gtcttgagaa
     1981 ggatgatcgc ctcgtccgcg acctcgccca ccgcgccgat gccggcgcga tattcggccg
     2041 tgacgttcag ggtcccggtg ggcagccgat ggccccgctc gccgtccccg aagcgcacgc
     2101 gggtgctgcc gtcgtcgtcg atcctcacct cgtaggcgcg gtcgtaggga cccgcgtcgt
     2161 agagcgaggc gacctcgtgc cagaggatcc cgtcgacccg cacctcgagc gtcgaggcgc
     2221 gcccttccgg ggtggcggcg gagacgaagg tgagcggctt cctggccagg gtgaagacct
     2281 ggttggcctt cgaggcgtcg cccgagccga gcgtgtcggc gaacgattcg ccgtgcgtgg
     2341 cgagcgccag gttcgcgttc acccggacgc tgctgcggat gtagctatag tccggcccgg
     2401 cctccagcag cagccgggtg tgcccgccga tgtggacgac gtcggcgacc gtcagagtct
     2461 ccgaatcggt gatcgtctcg gcgtcgctgc gctcgccgct gatcgagacg gcgcggccgc
     2521 gctcgatgtc
//
LOCUS       DASVQZ010000126         5370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_126699, whole genome shotgun sequence.
ACCESSION   DASVQZ010000126 DASVQZ010000000
VERSION     DASVQZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126699"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF547_03540"
     CDS             <1..587
                     /locus_tag="VF547_03540"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX8261929.1"
                     /translation="RSRAGDEDKLAVLDDAQAVFFSGGDQLRITSQIGDTGIEAKVRA
                     LYERGGVVAGTSAGASVMSETMLVKGTSKETHRIGDLHMSPGMGLIRDVIIDQHFAER
                     GRFGRLLGAVAHNPRVLGIGIDEDTAAMVEGDRFDVIGSGAVYVVDGTDVSYCNVAEA
                     RSDRALSMHDVRVHVLSDGDSFDLKARRPIHPRP"
     gene            624..1475
                     /locus_tag="VF547_03545"
     CDS             624..1475
                     /locus_tag="VF547_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261930.1"
                     /translation="MWKSATVELGSGRTLHAVRQGTGQDLLLLHGAMTTHHDWLAGSA
                     EALSQEGRVTVVDRPGHGLSRRPRFIGTPRDQAGQIAAGLDRLGIGAGVVVGHSFGAL
                     VALALAERFPERVTGLVLVAPLIFPEPRLVEHGLIAPRSMPVIGPLLSRLGRRSGFDR
                     AAIEWIQAVMFSPAPVPAEWKRSFPFEQVLDPDALVLEGEDAASILPLSPAGTIDLRR
                     IEVPAHILVGAEDRVVEQQRQGVALARLLRGGRLTEIEGAGHMLHHSHPDYVLRAVRE
                     AVLPVAA"
     gene            1472..2626
                     /locus_tag="VF547_03550"
     CDS             1472..2626
                     /locus_tag="VF547_03550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8261931.1"
                     /translation="MSGADFLVIGGGIAGLSAASRLAGHGKVVVLEAEEALGYHSSGR
                     SVSFSHYGIGNSAVRALTAWSRPFFEAPPEGFSPSPLAQTVPSLYFATEAALPALAAL
                     EAEMARFTDRTRRLDPAAMTALCPALRTGAGGAVGGVLDPTGLKLDSNALLHAFARAV
                     RASGGEIHNRRRIAAVERRGEGWRVRSEDGGEWSAPVLVNASGAWADRIAALAGVAPI
                     GLEPKRRTIIVVDPPTGTDVAGWPFAHSAAGDFYMLPEAGQLLVSPVDEVADDPCDAA
                     PDDYDVALAADRLEHYTGVPVTRIAHRWAGLRSFVADRTPTAGFDPDAPGFFWLVGQG
                     GYGLQTAPAMAAAVEALATGGPWPEGLADLGVLPEHVRPERLLSSRGRAT"
     gene            2743..4845
                     /locus_tag="VF547_03555"
     CDS             2743..4845
                     /locus_tag="VF547_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8261932.1"
                     /translation="MRLDQTAQWQPVPAQYQAPAGPAQPPPPPPPRRPWYHPLRWGWR
                     RIVLALLALFMLLIAWLAITAPLSKSLQPIAAPSLTLVSVEGIPIARRGADIREPVKI
                     AALPKHVPGAFIAIEDKRFHDHLGISVRGIARAAWRNLGAGGVREGGSTITQQLAKIS
                     FLSADRTAARKAQEVLIAFWLEAWLTKEEILERYLSNVYLGDNTYGLRAASWHYFSVP
                     PEKLTVPQAAMLAGMVKAPSRLAPTRNYKLARERGRVVIAAMIDQGFISRNEARKLKP
                     ARLRVERTKVVPTGTYFADWVLPMARARGEVGYGDQQVQTTLESRLQKLALQTVRRAG
                     LGRAQVALVAMRPDGRVVAMVGGRDYARSPFNRATQARRQPGSAFKLFVYLAALRAGM
                     TPDTPIADTPLRIGQWQPKNYGDRYRGTLTLRDAMALSSNVAAVRLSERVGRQNVIRA
                     ARDLGVRSPLNDNPSLALGTSGVTLLELTSAYAAVAAGAYPVQPRGLEEEEKPWWQRA
                     WRRATGTSSDPAFDELREMLGAVVSQGTGRGAALAVPAFGKTGTTQDSRDALFIGFAQ
                     DLVVGVWVGNDDNSPLAGQIAGGGLPARIWRDFMTGAVGTAPATKLAPPLQPPVPAES
                     FNGSITLPIEGGYEVGVQVDGNGVTVTGDGVPFPIEELPPRDEREPTDNPRRDPRDEP
                     GPPPRDDEPLPEDEEGAL"
     gene            4851..5234
                     /locus_tag="VF547_03560"
     CDS             4851..5234
                     /locus_tag="VF547_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX8261933.1"
                     /translation="MAGDAKEKLAEERTDLAHDRTVLANERTFAGWMRTGFAAVGIGL
                     AFQVLFQAMQPTWVPKAIATAFLLAGAYVIVVAERRAAAVMERFDEHKAAELRPINLK
                     VVTWTAVGATLALIAAIWLLEMKPA"
BASE COUNT          751 a         1951 c         1898 g          770 t
ORIGIN      
        1 agcgcagccg cgcgggcgac gaggacaagc tcgccgtgct cgacgacgct caggcggtgt
       61 tcttctccgg cggagaccaa ttacgcatca ccagccagat cggcgacacc ggcatcgagg
      121 ccaaggtgcg cgcgctctac gagcggggcg gggtcgtcgc cggcacctcg gccggagcgt
      181 cggtgatgag cgagacgatg ctggtcaagg gcacgagcaa ggagacccac cggatcggcg
      241 acctccacat gtcgcccggg atgggcctca tccgcgacgt catcatcgac cagcatttcg
      301 ccgagcgcgg ccggttcgga cggctgctcg gggcggtggc ccacaatccg cgggtgctgg
      361 gcatcggcat cgacgaggac acggcggcga tggtcgaagg cgatcgcttc gacgtgatcg
      421 ggagcggcgc cgtctacgtc gtcgacggca ccgacgtcag ctattgcaac gtcgccgaag
      481 cgcgctccga ccgggcgctt tcgatgcacg acgtccgggt ccacgtcctg tccgacgggg
      541 actccttcga cctcaaggcg cgtcgtccga ttcatccgcg cccttgagcg gagccatcgc
      601 cgcgaccggc ggttgaggcg gccatgtgga aatcagcaac cgtcgaactc ggctccggcc
      661 ggacccttca cgccgtccgc caggggaccg gccaggacct gctgctgctc cacggggcca
      721 tgaccaccca ccatgactgg ctggcgggat cggccgaggc gctttcgcag gagggccgcg
      781 tcacggtcgt cgaccgaccg gggcacgggc tcagccgccg tccgcgcttc atcgggaccc
      841 cccgcgacca ggcgggacag atcgccgcgg gcctcgacag gctcggcatc ggcgccgggg
      901 tcgtcgtcgg ccacagcttc ggagcgctcg tcgccctcgc cctggccgag cgtttcccgg
      961 agcgggtcac cggactcgtg ctcgtcgccc cgctcatatt tccggagccg cgactggtgg
     1021 agcacgggct gatcgcgccg cgctcgatgc cggtgatcgg gccgctgctc tcccggctgg
     1081 gccggcgcag cggcttcgac agggcggcga tcgaatggat ccaggcggtg atgttctccc
     1141 ccgcgccggt gccggctgaa tggaagcgca gctttccgtt cgagcaggtt ctcgaccctg
     1201 acgctctcgt cctcgaaggc gaggacgccg cctcgatcct gccgctctcg ccggcgggaa
     1261 cgatcgacct tcgccggatc gaggtcccgg cccacatcct cgtcggggcc gaggatcgcg
     1321 tggtcgagca gcagcgacag ggcgtggcgc tggcgcggct cttgcgcggt ggccgcctca
     1381 ccgagataga gggggccggc catatgctcc atcacagcca ccccgattac gtccttcgcg
     1441 ccgttcgcga ggccgtcctt cccgtcgccg catgagcgga gccgacttcc tggtcatcgg
     1501 cggcggcatc gccgggctct cggcggcgtc gcggctcgcc ggtcacggca aggtcgtcgt
     1561 cctcgaggcg gaggaggcgc tcggctatca cagctccggc cggagcgtgt cgttcagcca
     1621 ttacggcatc ggcaattcgg cggtgcgcgc gctcaccgcc tggagccggc ccttcttcga
     1681 ggcgccgccc gagggcttct cgccctcccc gctcgcccaa accgtcccct cgctctactt
     1741 cgcgaccgag gcggcgctgc cggcgctggc ggcgctggag gccgagatgg cgcgcttcac
     1801 cgaccggacc cggcggctcg acccggcggc gatgacggcg ctgtgcccgg ccttgcgcac
     1861 cggcgccggc ggagccgtcg gcggagtgct cgacccgacc ggcctcaagc tcgattcgaa
     1921 cgccctcctc cacgccttcg cccgcgccgt ccgggcgagc ggcggcgaga tccacaaccg
     1981 ccggcggatc gcggccgtcg agcggcgggg cgagggctgg cgggtgcgca gcgaggacgg
     2041 cggggaatgg tcggcgcccg tcctggtcaa cgcgtccggc gcctgggccg accggatcgc
     2101 ggctctcgcc ggagtggcgc cgatcggcct tgagcccaag cgcaggacga tcatcgtcgt
     2161 cgatcccccg accggaaccg acgtcgccgg ctggcccttc gcccacagcg cggccggcga
     2221 cttctacatg ctgcccgagg cgggacagct gctggtctcg ccggtcgacg aggtggcgga
     2281 cgatccctgc gacgcggcgc ccgacgacta tgacgtggcg ctcgcggcgg accggctcga
     2341 acattatacc ggcgtgccgg tgacccggat cgcccatcgc tgggccgggc tccgctcgtt
     2401 cgtggccgac cggaccccga ccgccggctt cgacccggac gctcccggct tcttctggct
     2461 ggtcggacag ggcggctacg gcctccagac cgcgccggcc atggcggcgg ccgtcgaagc
     2521 cctggccacc ggcgggccct ggcccgaggg gctggccgac ctcggcgtgc ttccggagca
     2581 cgtccggccg gaacggctgt tatcttcgcg agggcgcgca acctgattcc cgcctgatcg
     2641 ttccccggcg aaggccgggg tccagtcttc ccgactggcg ccgctgggcc ccggccttcg
     2701 ccgggagctt acctggggcg cgagatgctc taagaagaag gaatgcgttt ggaccagacc
     2761 gcccagtggc aacccgtccc ggcccagtat caggccccgg cgggccccgc tcagccccct
     2821 ccgccgccgc cgccgcgccg gccctggtac cacccgctca gatggggctg gcggcggatc
     2881 gtcctcgccc tgctcgcctt gttcatgctg ctcatcgcct ggctggcgat caccgcgcct
     2941 ttgtccaagt cgctccagcc gatcgccgcg cccagcctca ccctggtctc ggtcgagggg
     3001 attccgatcg ctcggcgcgg cgcggacatc cgggagccgg tcaagatcgc cgcccttccc
     3061 aagcacgtcc cgggcgcctt catcgcgatc gaggacaagc gcttccacga ccatctcggg
     3121 atcagcgtgc gcggaatcgc ccgcgccgcc tggcgcaacc tcggcgccgg cggagtccgc
     3181 gagggcggga gcacgatcac ccagcagctc gccaagatct ccttcctcag cgcggaccgg
     3241 accgccgcgc gcaaggcgca ggaggtgctg atcgccttct ggctggaggc ctggctgacc
     3301 aaggaggaga ttctcgagcg ctatctctcc aacgtctatc tcggcgacaa cacctatggg
     3361 cttcgggccg cgtcctggca ttatttcagc gttccgcccg agaagctgac cgtgccccag
     3421 gcggcgatgc tggcgggaat ggtcaaggcg ccgtcgaggc tcgcgcccac ccgcaactac
     3481 aagctggcgc gcgagcgggg ccgggtcgtg atcgcggcga tgatcgacca gggcttcatc
     3541 agccgcaacg aggcgcggaa gctgaagccg gcccggctca gggtcgagcg gaccaaggtg
     3601 gtccccaccg gcacctattt cgcggactgg gtgctgccga tggcgcgcgc ccgcggcgag
     3661 gtcggctatg gcgaccagca ggtccagacg accctcgaga gccggcttca gaagctggcg
     3721 cttcagaccg tgcgccgggc cggcctcggc cgggcccagg tcgccctggt cgcgatgcgg
     3781 cccgacgggc gcgtcgtcgc catggtcggc ggccgcgatt atgcgcgcag cccgttcaac
     3841 cgcgccaccc aggcccggcg ccagccgggc tcggcgttca agctgttcgt ctatctcgcc
     3901 gccctgcgcg ccggcatgac ccccgacacg ccgatcgccg acacgccgct tcggatcggc
     3961 cagtggcagc cgaagaacta cggcgaccgc tatcgcggca cgctcaccct gcgcgacgcg
     4021 atggcgctgt cgagcaacgt tgcggcggtg cggctgtcgg agcgggtcgg gcgccagaac
     4081 gtgatccgag ccgcccgcga cctcggcgtg aggagccccc tcaacgacaa tcccagcctc
     4141 gccctgggga cttcgggcgt cacgttgctc gagctgacct cggcctatgc cgccgtcgcc
     4201 gcgggcgcct atccggtgca gccgcgcggc ctcgaggagg aggagaagcc ctggtggcag
     4261 cgcgcctggc ggcgggcgac ggggaccagc agcgaccccg ccttcgacga gctcagggag
     4321 atgctcggcg ccgtcgtcag ccagggcacc ggccgaggcg cggcgctcgc cgtcccggcc
     4381 ttcggcaaga cggggacgac tcaggacagc cgggacgcct tgttcatcgg cttcgcccag
     4441 gatctggtcg tcggagtctg ggtgggcaat gacgacaaca gcccgctcgc gggccagatc
     4501 gccggcggcg ggctcccggc ccggatctgg cgcgacttca tgaccggcgc ggtcgggacg
     4561 gcgccggcga cgaagctggc gccgccgctt cagccgccgg tcccggccga gagcttcaac
     4621 ggctccatca ccctcccgat cgagggcggc tatgaggtcg gggtccaggt ggacgggaac
     4681 ggcgtgaccg tcaccggcga cggcgtcccc ttcccgatcg aggagctgcc gccgcgcgac
     4741 gagcgcgaac cgaccgacaa tccgcgccgc gacccgcgcg acgagccggg gccgccgccc
     4801 cgcgacgacg agcccctgcc cgaagacgaa gaaggcgcgc tctgaggccg atggcgggcg
     4861 acgcgaagga aaagctcgcc gaggagcgca cggacctcgc ccacgaccgg acggtcctcg
     4921 ccaacgagcg gaccttcgcg ggatggatgc gaaccggctt cgccgcggtc gggatcgggc
     4981 tcgccttcca ggtcctgttc caggcgatgc agccgacctg ggtgccgaag gcgatcgcga
     5041 ccgccttcct gctcgccggc gcctatgtga tcgtcgtcgc cgagcggcgc gccgccgccg
     5101 tcatggagcg gttcgacgag cacaaggccg ccgagcttcg gccgatcaac ctcaaggtgg
     5161 tgacgtggac ggccgtcggc gcgacgctcg cgctgatcgc ggcgatctgg ctgctggaga
     5221 tgaagccggc ctagagcatg atcgcgcttc gttgaatcga aaggggattc ccaagacggc
     5281 tggcttctga ttcaacatgc tggctgggag gaggccagca tggatgaggc cttattcgac
     5341 tgatcttcgg gagcgcgtgg ttgcggcgat
//
LOCUS       DASVQZ010000127        13614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_894933, whole genome shotgun sequence.
ACCESSION   DASVQZ010000127 DASVQZ010000000
VERSION     DASVQZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894933"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VF547_03565"
     CDS             complement(<1..1284)
                     /locus_tag="VF547_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8261934.1"
                     /translation="MRRLLLPFALLSAFAVAACASTASDEIATASDAPPVQEASAAAP
                     AGPAPVAELVRKIDIPYEQFTLDNGLRVIVHEDRKAPVVAISIWYNVGSKDEPKGRTG
                     FAHLFEHLMFNGSENAPGDYFEPLREIGATDFNGTTWFDRTNYFQTVPKPALERGLFL
                     EADRMGRLLGAVTQEKLTNQIGVVQNEKRQGDNEPYGLVEYAQLEALFPEGHPYRHST
                     IGSMADLDAATLEDVKNWFRSKYGPNNAVLVLAGDVSAAEARPLVEKHFGGIPRGPVN
                     NPAKADVPTLPAPKVEVMQDRVANVRLYRNWIVPGMLDDAAVQLDVAAGVLGGLASSR
                     LDNELVRKDQTAVGVRAYILPFHRVSQFEIQVDVKPGVDPEIVSKRLDEIVAELIANG
                     PTADEVQRVVMTDVAGRIRGLEQVGGFGGKAVALAE"
     gene            1427..3037
                     /locus_tag="VF547_03570"
     CDS             1427..3037
                     /locus_tag="VF547_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEX8261935.1"
                     /translation="MRFKAFGPALVSLALASPAPGQPAEFRPESFRSHVTFLADDRLE
                     GRDSGTRGYDIAALYVASRFEAIGLKPVGPAGWYQQVPFLEYRLTGEPASLTIGGRRF
                     LHGQEVILTPTPEETPVAVEAPAVFVGYGLVAPQLGIDDYKGLDVRGKVVVALNGFPK
                     DLKSDVGAHLNSEKRRVASERGAVGLVLIRTRQEAKRRLWSRLQENRNEPAMTWTGGD
                     GRPYSPAPGLRFRATLDSAAAEALFAGARTPLQAVLDQAERGGARPSGFALKPRIAVS
                     QRSSQRRFRSPNVLAVLPGSDPKLAREYVLLMAHLDHEGVKPEGEGDRIYNGAMDNAV
                     GIATMIEVARAMAASPDRPKRSILFAAVTAEEDGLLGAQYLARHPVPGGKVAAVVNLD
                     MPVLLYDFTDVVAFGAEHSTMGKAVERASRGMGVALSEDPLPHEGLFTRSDHYRFVQE
                     GVPSVFLMTGFANGGREKFTDFLARHYHKVSDQLDLPIDWRAGAKFARINYEIAREIA
                     DAPEAPRWYSDSFFGKALAPRQEKAARE"
     gene            3127..4941
                     /locus_tag="VF547_03575"
     CDS             3127..4941
                     /locus_tag="VF547_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8261936.1"
                     /translation="MKSDTPAAVRRMERSPAARTAAPAPTSLPEPLKSGAEALGGFSL
                     ADVKVHRDSPEPAELGALAFTRGGDIHLATGQELHLPHETWHAVQQKQGRVSATAQVR
                     GQRLNGDDRLEAEADRIGSVAAAMTSTRPGATEARRLSHDGAVVQRRPRRSRLLSATI
                     DVKWSENEDQFYDRVLAALARSAEFAGLDSGTYNSASATEHMLYDLVRAFRSQYHEAF
                     GRRPKPGEAVKLHLEGFYDAGEATLVGKKVSFVSDAARTDAPRQRSLEAPSDCGKRRD
                     ATETDIQRETREIDESSCFIAREILTGALQNAASVSITLTSAGTFPAGVSFAGSGEPR
                     SKASPIAYAEAYRVAREAVETFHVATSHTPSTMTVDFSLAGGVTMMRQTITPLPADIE
                     SADPVEGADDGECLEEDIDYGECIAMERSDAYREGIAAAEEEFKRWYDPYNLSGDNSG
                     LPPRPPGKFPKGIKPSKVNKLRKINKAAPMAMKEIAGKVHGIRGRLRGQAIAVVEVQV
                     GTTVRYAAGKNSGSTWSRRQTNLLNDLGIEKIPSHLSESVHAEANVRAWVQSLERQFG
                     RQNVRVKRWGISAGRDGKFICGACREIARQVGGIIETF"
     gene            5095..7692
                     /gene="clpB"
                     /locus_tag="VF547_03580"
     CDS             5095..7692
                     /gene="clpB"
                     /locus_tag="VF547_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX8261937.1"
                     /translation="MNLDKFTERARGFLQAAQTVALRLNHQRIAPAHLLKALLEDEQG
                     MASGLIARAGGSAEAARRETDEALTRIPVVTGGGANSTPGLDAETLRVLDQAEQVAQK
                     AGDSYVTVERLLLALVLAKTTDAGKALADAGVKAEALNAAINELRGGRTADTQSAEDR
                     YDALKKFARDLTQAARDGKLDPVIGRDEEIRRTVQILARRTTNNPVLIGDPGVGKTAI
                     AEGLALRIANGDVPDSLKDRKLMGLDMGALIAGAKYRGEFEERLKGVLDEVRQGEGDI
                     ILFIDEMHTLIGAGKSEGAMDASNLLKPALARGELHCIGATTLDEYKKHVEKDPALQR
                     RFQPVFIGEPTVEDTISILRGLKEKYELHHGVRITDGALVAAATLSNRYITDRFLPDK
                     AIDLMDEAASRLRMEVESKPEEIENLDRRIIQLKIEREALKKEKDRASQERLKTLEEE
                     LANLEEQSAEITQRWQAEKEKLAGEAKIKEELDKVRLELDQAQRAGDLAKAGELQYGR
                     IPQLEKQLAEVEAASATAMLREEVTSEDIASVVARWTGIPMERMLEGEREKLLNMEAV
                     LGRRVIGQEDAVRAVAKAIRRSRAGLQDPNRPMGSFLFLGPTGVGKTELTKSLAEFLF
                     DDPHAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGVLTEAVRRRPYQVVLFDEVEK
                     AHSDVFNVLLQVLDDGRLTDGQGRTVDFTNTIIILTSNLGSQYLTGLGEGQTVADVES
                     QVMEVVRGHFRPEFLNRLDEIILFHRLGQDHMGPIVDIQVERVQQLLADRKIRLELTD
                     SARAWLGRVGYDPVYGARPLKRAVQRYLQDPLADALLRGEIPDGTNVAVDEGEGALTL
                     RTGERREAA"
     gene            complement(7689..9185)
                     /locus_tag="VF547_03585"
     CDS             complement(7689..9185)
                     /locus_tag="VF547_03585"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX8261938.1"
                     /translation="MSETALAIARVDPADSTSAYNAGLKALKAGLEPEGLALLEAARR
                     SAPGDARLWQVAGLLHRQLEDLEPAIAMMTRAAELAPRDPLIAHTLARVHLEAGLPSV
                     ELFEAARRLAPHDGEILLGLVAAVEDIRGPAAAIVALERLLSGRPGWVAGLELLMRLR
                     WLCGDREGFTADFERALAADPRNIGLWRSLITALTRGDQYELALDAVRRGREGAGPHL
                     IFDANEALCRSELGQTDLADRLFARLAALDDPILRVRHVRHLLRDGRVAEAGALAEKS
                     IGSPAGHVFWPYLSIAWRLLGDPRWQWLEGDERFVGVYDLDLPSLDALAERLRTLHRT
                     THQPLEQSVRGGTQTQGNLFARVEPEIRVLRAAVVDAVERHIGQLPPPQPDHPLLGAP
                     RGRRVRFAGSWSVRLTGGGHHANHIHPAGWFSSALYVALPAQAQPPAGWLTLGVPQAE
                     LGIDLPPIRTVEPKPGRLVLFPSTLWHGTVPFEAGERLTVAFDVARPV"
     gene            complement(9182..10339)
                     /locus_tag="VF547_03590"
     CDS             complement(9182..10339)
                     /locus_tag="VF547_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8261939.1"
                     /translation="MDAELSRAEAEADAAAQAGRLGDARRLLEAAVAETPDRLGTWLK
                     LAAMCRGTGDLGAAMTAVSRALALDPVDLSALLVRAMLLERMGKADEAGEAYGHALAQ
                     RPAGDDPPGLAPMLAHAEQSYARFQEALAGRLRGGLAGGGTTPAEQARIDRFVTNVAR
                     ITRPFAQEPSHFHFPGLPPVEFHDRDLFPWLGELEAATPEIREEFDALIASEAAELVP
                     YIQYPDDVPVRQWQALNRSRDWTAVHLLQNGRRIERNARHCPRTMALLGRLPQPDVPG
                     ASPNAMFSLLAPRTRIPPHTGVANTRLVCHLPLIVPAGCGFRVGAQTRDWRPGTAFVF
                     DDTIEHEAWNDSPELRVVFIFDVWAWALSAAERRAVAAIVPRSGAAGAEGL"
     gene            10439..11890
                     /locus_tag="VF547_03595"
     CDS             10439..11890
                     /locus_tag="VF547_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8261940.1"
                     /translation="MGAGTTPEELLRIASQHREAGRVAEAIGAYEAALALRPGLPNSW
                     YNLARLQRQAGRFEAALASYAQALAHGVSEPEEVHLNRGVIYADDLARSDAAEAELAK
                     ALALNPRYVPALLNLGNLHDDRGDRTSARSAYERALAIEPDNVIALARLAGAAPLSGP
                     DDPLLGRLRTGIARAGADPAARADLGFALGAALDRLGAYDEAFAAFAAANEASRQSSP
                     VRYDRRAQERFVDRMIESFPEPARTAAAPGGEAPLFICGMFRSGSTLAEQILARHEDV
                     TPGGELGLVTALAARLRPYPEAAAAAGPEALAEWRSDYLARLRRIHPGGGLVTDKRPD
                     NFLHIGLIKSLFPEARIVHTRRSPPDNVLSLYFLHLDPAMAYARDLGDAAHWYGQYRR
                     LMAHWRRLYGDDIHDLDYDALVADPRPHIEALLGFCGLSWREACMSFHEAGNAVRTAS
                     AAQVREPLYRRSSGRWRNYERHLGGLIEALRDV"
     gene            complement(11959..>13614)
                     /locus_tag="VF547_03600"
     CDS             complement(11959..>13614)
                     /locus_tag="VF547_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8261941.1"
                     /translation="GDLGNGFSYDASYQYGRVVFAQTYFNDFSVTRIGRALDVVTGPN
                     GTPVCRSVLTGIDANCVPYDIFALGGVTQAALDYLQTPGFSRGNTQETVATGSITADL
                     GEHGVQSPWSESGVAVNVGAEYRKERLEFSTDQAFSTGDLAGQGGATIGVTGEFDVKE
                     LFTELQIPLISDRPFFEQLEFRAGYRYSKYDVANNSFSTDTYKFEGEWAPVRDIRFRG
                     SYNRAVRAPNIVELFSAQAVGLSGSTDPCAGPTPAATQAQCVLADLPAAQYGKVPINP
                     AGQYNSLLGGNPNLTPETADSITLGVVLQPRFLPRFALTVDYFDIRLDNAIGIIGADT
                     ILAQCVATGDPFFCGKINRDAAGSLWLTPNGFTVDTNTNVGGLSTRGVDVNASYTQPL
                     GGMGSVAFSFVGTWLDELIVNPLADIKFNCAGFFGGQCGTPSPEWRHKFRVSYTHPSG
                     IGLSAQWRYFSAVDNDLTSADPDLAGAVNEGDRHFGSQSYIDLVLTARIGDHYSFRLG
                     ANNILDRIPPIAVTPAPFGNGNTYPQVYDSLGRYIFAGVTLDF"
BASE COUNT         2259 a         4570 c         4825 g         1960 t
ORIGIN      
        1 ctcggcgagg gcgacggcct tgccgccgaa gccgccgacc tgctccagcc cgcggatgcg
       61 gccggcgacg tccgtcatga cgacgcgctg gacctcgtcc gcggtcgggc cgttggcgat
      121 cagctcggcg acgatctcgt cgagccgctt cgacacgatc tcgggatcga ccccgggctt
      181 cacgtcgacc tggatctcga actggctgac ccggtggaag ggcaatatgt aggcgcgcac
      241 gccgaccgcc gtctggtcct tgcggaccag ctcgttgtcg aggcgcgagc tggcgaggcc
      301 gccgagcact ccggccgcga cgtcgagctg cacggccgca tcgtcgagca tgcccggcac
      361 gatccagttg cgatagaggc ggacgttggc gacgcgatcc tgcatcacct cgaccttggg
      421 cgccggcagg gtcggcacgt cggccttggc cgggttgttg acgggcccgc gcgggatgcc
      481 gccgaaatgc ttctcgacca agggccgggc ctcggcggcc gagacgtcgc cggcgaggac
      541 cagcacggcg ttgttcggcc cgtatttcga gcggaaccag ttcttcacgt cctcgagagt
      601 cgccgcgtcg agatccgcca tcgagccgat cgtcgaatgg cgatagggat ggccttccgg
      661 aaacagcgcc tcgagctggg catattcgac gaggccgtag ggctcgttgt cgccctggcg
      721 cttctcgttc tggacgactc cgatctggtt ggtcagcttc tcctgggtca ccgcgccgag
      781 cagccggccc atccggtccg cctcgaggaa caggccgcgc tcgagcgcgg gcttcggcac
      841 ggtctggaaa taattggtgc gatcgaacca ggtggtgccg ttgaagtcgg tcgccccgat
      901 ctcgcgcaga ggctcgaaat aatcgccggg cgcgttctcc gagccgttga acatcagatg
      961 ctcgaacagg tgcgcgaagc cggtccggcc cttcggctcg tccttcgagc cgacattgta
     1021 ccagatggag atggcgacga ccggcgcctt gcggtcctcg tggacgatca ccctgaggcc
     1081 gttgtcgagg gtgaactgct cgtaggggat gtcgatcttg cggaccagct cggcgaccgg
     1141 ggccgggccg gccggcgcag cggcggacgc ttcctggacg ggcggagcgt ccgaggcggt
     1201 cgcgatctcg tcggaagcgg tcgacgcgca agcggcgacg gcgaaagcgg agaggagagc
     1261 aaaggggagc aacaggcggc gcattcggca gtccttcccg agtcttgtcc ggcggcacca
     1321 tagcggcccg ccttgacggc gcaatatgcg gcgattgccc aagcgctttt gacgggccgc
     1381 cgatctgctg tagggagcgg cctgcctcga tccccaggaa ttccccatgc gcttcaaagc
     1441 tttcggcccc gccctcgtca gcctcgccct cgcctcgccg gctcccggcc agccggcgga
     1501 gttccggccg gaaagctttc gcagccacgt caccttcctc gccgacgatc ggctcgaggg
     1561 gcgggattcc ggcactcggg gctatgatat cgcggcgctc tacgtggcga gccgcttcga
     1621 ggcgatcggc ctgaagccgg tggggccggc cggctggtac cagcaggttc ccttcctcga
     1681 ataccgcctg accggcgagc ccgcgtcgct caccatcggc gggcggcgct tcctgcacgg
     1741 ccaggaggtg atcctgacgc cgacgccgga agagactccg gtcgcggtcg aggcgcctgc
     1801 ggtcttcgtg ggctacgggc tcgtcgcgcc gcagctcggg atcgacgact acaagggcct
     1861 cgacgtccgc ggcaaggtgg tggtcgcctt gaacggcttc cccaaggact tgaagagcga
     1921 cgtcggcgcc catctcaatt ccgagaagcg gcgggtcgcg tcggagcgcg gggccgtcgg
     1981 gctggtcctc atccgaacgc gccaggaagc gaaaaggcgg ctctggtcgc ggctgcagga
     2041 gaacaggaac gagccggcca tgacctggac cggcggggac gggcgcccct acagcccggc
     2101 gccgggcctg cgcttccgag cgacgctcga ttcggccgcc gccgaggcgc tcttcgccgg
     2161 agcgcggact ccgctgcaag ccgtcctcga ccaggccgag cgcggcgggg cccggcccag
     2221 cggcttcgct ctcaagccga ggatcgcggt cagccagcgc agcagccagc ggcgcttccg
     2281 cagccccaac gtgctcgccg tcctccccgg atcggacccg aagctggccc gagaatatgt
     2341 gttgctgatg gcgcatctcg accacgaagg cgtcaagccc gaaggcgagg gcgaccgcat
     2401 ctacaacggc gcgatggaca atgcggttgg gatcgccacc atgatcgagg tcgcccgggc
     2461 gatggccgcc tcgccggatc ggcccaagcg ctcgatcctg ttcgcggccg tcaccgccga
     2521 ggaggacggc ttgctgggcg cgcaatatct ggcccgccac ccggtgcccg gcggcaaggt
     2581 cgcggcggtc gtcaacctcg acatgccggt cctgctctac gacttcaccg acgtggtcgc
     2641 gttcggcgcc gagcattcga cgatgggtaa ggcggtcgag cgggcgagcc ggggcatggg
     2701 cgtcgcgctc tccgaggacc cgctgccgca cgagggcctc ttcacgcgct ccgaccatta
     2761 ccgtttcgtc caggaagggg tcccgtcggt cttcctgatg accggcttcg ccaacggcgg
     2821 gcgggagaag ttcaccgact tcctcgccag gcactaccac aaggtgagcg atcagcttga
     2881 cctgccgatc gactggcggg ccggtgccaa atttgcccgg atcaactacg agatcgcccg
     2941 cgagatcgcc gatgcgcccg aggcgccgcg ctggtattcc gacagcttct tcggcaaggc
     3001 gctggcgccg cgccaggaga aggctgcccg ggagtaagag actcgacttg ccgccagcgg
     3061 aacgcatacg gtcagccaac cgtggaggcg ccgatgcgct gcagggtccg ttgcccgccg
     3121 gcccagatga agagcgacac cccggctgcg gtgcggagaa tggagcgctc gcctgccgct
     3181 cgcaccgccg ctccggcacc gacgtcgctc ccggagccat tgaagtccgg cgccgaagcg
     3241 ctcggcggct tctccctggc cgacgtgaag gtccatcgcg attcgcccga gccggctgag
     3301 cttggcgcgc tcgccttcac ccgcggcgga gacatccacc tcgcaaccgg ccaggagctc
     3361 catctccccc acgaaacctg gcatgcggtc cagcagaagc agggacgggt gagcgcaacg
     3421 gcccaggtga ggggtcagcg cctcaacggc gacgaccggc tcgaggccga ggcggatcgg
     3481 attggctcgg tcgcagcggc gatgacatcg acgagaccgg gagcgaccga agcgcgccgc
     3541 ctaagccatg atggcgcggt cgttcaacgg cgtccgaggc ggtcgcgtct cttgtcagcg
     3601 acgatcgacg tcaaatggtc ggaaaacgag gatcagtttt acgatcgagt gctggccgcg
     3661 cttgcgcgaa gcgccgaatt cgcagggctc gattccggga cctataattc cgcaagcgcg
     3721 accgagcaca tgctttacga tctggtgcgt gcattcaggt cccaatatca tgaggctttc
     3781 ggtcggcgtc ctaagccggg cgaagcggtc aaattgcacc tcgaaggttt ctacgatgcc
     3841 ggcgaagcca ccctcgtcgg aaagaaagtc tcgttcgtat ccgacgccgc gaggaccgac
     3901 gcgccgcggc agcggtcgct ggaggctccg agcgactgcg ggaaaaggcg cgatgcgacg
     3961 gaaaccgata tccagcgcga gacgcgcgaa atcgatgaga gcagctgttt catcgcacga
     4021 gagatattga ccggcgctct gcagaacgcc gcatcggtca gcataacgct cacttctgca
     4081 ggcacttttc ctgccggagt gagctttgcg ggttctggtg agcctagatc gaaggcctcg
     4141 ccgatagcct acgcagaggc ttaccgggtc gctcgcgagg ccgtggagac atttcacgta
     4201 gcaacgtcac acacgccgag caccatgacc gtcgattttt cgctcgcggg aggagtgacg
     4261 atgatgcgtc agacaatcac tccgctcccg gccgacatcg aatccgccga cccagtggag
     4321 ggcgcggacg atggcgaatg tctcgaagag gatatcgatt atggcgaatg tatcgcgatg
     4381 gagcggtcgg acgcctatcg cgaaggaatt gctgcggccg aggaagagtt caagcggtgg
     4441 tacgacccct ataatctctc gggcgataac agtggcttgc ctccgaggcc gcctggaaag
     4501 tttccgaaag gcatcaagcc ctcgaaggtc aataagctca ggaagataaa caaggccgcg
     4561 ccgatggcga tgaaggagat cgcgggcaag gttcacggca tacgcggacg gctcagagga
     4621 caggcaatcg cggtcgtgga ggtgcaggtc gggacgacgg ttcgctacgc ggcggggaag
     4681 aattcaggca gtacatggag ccgacgccag acgaatctgc tgaatgacct cggcatagag
     4741 aaaattccct cgcatctctc cgagagcgtg catgcggaag cgaatgtgcg ggcttgggta
     4801 cagagcctgg aaaggcagtt cggccggcag aacgtacgcg tcaagcggtg gggaatcagt
     4861 gcgggccggg acggtaaatt catttgcggt gcctgccgcg agatagcgcg gcaagtcggc
     4921 ggaatcatcg agacgttttg aggattccga gcctgcgcag cgccgcggaa ggaaggtcgc
     4981 gccgggttgg gcggcatgcc gatgccggcg ctgggaagag gtccggttca ccagctcgga
     5041 cacttgtgtg gcccgcgggc aacactatat cgcgccacga aacgaaggcg agctatgaac
     5101 ctggacaaat tcaccgagcg ggcgcggggc ttcctgcagg cggcacagac cgtggcgctt
     5161 cgcctcaatc atcagcgtat cgctccggcg caccttctga aggccttgct cgaggacgag
     5221 cagggcatgg cctccggact gatcgcgcgg gccggcggca gcgccgaggc ggcgcggcgg
     5281 gaaaccgacg aggcgctgac tcggataccg gttgtcaccg gcggaggcgc caattcgacg
     5341 cccggcctcg acgccgagac cctgcgggtg ctcgaccagg ccgagcaggt cgcgcaaaag
     5401 gccggcgaca gctacgtgac ggtagagcgg cttctgctcg cgctcgtgct ggcgaagacg
     5461 acggacgccg gcaaggcgct cgccgacgcc ggggtcaagg ccgaggccct caacgcggcg
     5521 atcaacgagc ttcgcggcgg ccggaccgcc gacacccagt cggccgagga ccgctacgac
     5581 gcgctcaaga agttcgcgcg cgacctcacc caggcggcgc gcgacggcaa gctcgacccg
     5641 gtgatcggcc gcgacgagga gatccgccgg accgtccaga tcctcgcccg gcggaccacg
     5701 aacaatccgg tgctgatcgg cgatcccggc gttggcaaga ccgcgatcgc cgaagggctg
     5761 gcgctgcgca tcgccaatgg cgacgtcccc gacagcctca aggaccgcaa gctgatgggg
     5821 ctcgacatgg gcgcgctgat cgccggcgcc aaatatcgcg gcgagttcga ggagcggctg
     5881 aagggcgtgc tcgacgaggt ccgccagggc gagggcgaca tcatcctgtt catcgacgag
     5941 atgcatacgc tgatcggggc cggcaagtcc gagggcgcga tggacgcgtc caacctcttg
     6001 aagccggcgc tggcgcgcgg cgagctccac tgcatcggcg cgaccacgct cgacgaatat
     6061 aagaagcatg tcgagaagga cccggcgctc cagcggcgct tccagcccgt cttcatcggc
     6121 gagccgacgg tcgaggacac gatctccatc ctgcgcggcc tcaaggagaa atacgagctc
     6181 caccacggcg tccggatcac cgacggcgcc ctcgtcgcgg cggccacgct ctcgaaccgc
     6241 tacatcaccg accgcttcct tcccgacaag gcgatcgacc tgatggacga ggccgcctcg
     6301 cgcctgcgca tggaggtgga atccaagccc gaggagatcg agaacctcga ccggcgcatc
     6361 atccagctca agatcgagcg ggaggcgctg aagaaggaga aggaccgggc ctcgcaggag
     6421 cggctcaaga cgctcgagga ggagctcgcc aatctcgagg agcaatccgc cgagatcacc
     6481 cagcgctggc aggcggagaa ggagaagctg gccggcgagg cgaagatcaa ggaggagctg
     6541 gacaaggtcc ggctcgagct cgaccaggct cagcgcgcgg gcgacctcgc caaggcgggc
     6601 gagctccagt acggccgcat cccgcagctc gagaagcagc tcgccgaggt cgaggcggcg
     6661 agcgcgacgg cgatgcttcg cgaggaggtc accagcgagg acatcgcctc ggtggtcgcg
     6721 cgctggaccg gaatcccgat ggagcggatg ctggagggcg agcgcgagaa gctgctcaac
     6781 atggaagccg tgctcggccg gcgggtgatc ggccaggagg atgcagtgcg cgccgtcgcc
     6841 aaggcgatcc gccgctcccg cgccggcctt caggacccga accggccgat gggcagcttc
     6901 ctgttcctgg gacccaccgg agtcggcaag accgagctga ccaagtcgct ggccgaattc
     6961 ctgttcgacg atccgcacgc gatggtccgg atcgacatgt ccgagttcat ggagaagcac
     7021 agcgtcgccc ggctgatcgg cgcgcccccc ggctatgtcg gatatgagga aggcggcgtg
     7081 ctcaccgagg cggtgcgcag gcggccctac caggtggtgc tgttcgacga ggtcgagaag
     7141 gcccattcgg acgtcttcaa cgtcctcctc caggtgctcg acgacggccg gctgaccgac
     7201 ggccagggcc gcaccgtcga cttcaccaac acgatcatca tcctcacctc gaacctgggc
     7261 agccagtatc tgaccgggct cggcgaaggc cagaccgtcg ccgatgtcga atcgcaggtg
     7321 atggaggtcg tccgcggcca tttccgcccc gaattcctca accggctcga cgagatcatc
     7381 ctcttccacc ggctcggcca ggaccatatg ggcccgatcg tcgacatcca ggtcgagcgc
     7441 gtccagcagc tgctcgccga ccgcaagata aggctcgagc tgaccgattc ggcgcgggcc
     7501 tggcttggac gggtcggcta cgacccggtc tacggcgcgc gccccttgaa gcgggcggtg
     7561 cagcgctatc tgcaggatcc gctcgccgac gccctgctca ggggcgagat acccgacggc
     7621 accaacgtcg ccgtcgacga gggcgagggc gctctcaccc tgcggaccgg cgagcggcgc
     7681 gaggcggcct agaccgggcg ggcgacgtcg aaggccacgg tgaggcgctc gcccgcttcg
     7741 aacgggacgg tgccgtgcca cagcgtcgag gggaagagca ccaggcggcc gggcttcggc
     7801 tcgacggtgc gtatcggcgg caggtcgatg cccagctcgg cctgtgggac gcccagcgtg
     7861 agccagccgg ccgggggctg cgcctgcgcc ggaagcgcga cgtagagcgc agagctgaac
     7921 cagcccgccg gatggatgtg gttggcatga tggccgccgc cggtgagccg caccgaccag
     7981 gagccggcga accgcacccg ccggccgcgc ggcgccccga gcagcggatg gtccggctgc
     8041 ggcggcggca attggccgat atggcgctcc acggcgtcga ccacggcggc gcgaagcacc
     8101 cggatctccg gctcgacccg agcgaacaga ttgccctgcg tctgggtgcc gccgcgaacc
     8161 gattgctcga gcggctggtg ggtggtgcgg tgcagggtcc tgaggcgctc cgcgagggcg
     8221 tcgagcgagg gcaggtcgag gtcgtagacg ccgacgaacc gctcgtcccc ctccagccac
     8281 tgccagcgcg gatcgcccag cagtcgccag gcgatcgaca gatagggcca gaacacgtgc
     8341 ccggccggag acccgatgga tttctcggcc aaagcccccg cctccgcgac acggccgtcg
     8401 cgaagcaggt ggcggacatg gcggacgcgc aggatcggat cgtcgagcgc ggcgagccgg
     8461 gcgaacagcc ggtcggcgag gtcggtctgg ccgagctcgg agcggcacaa ggcctcgttc
     8521 gcgtcgaaga tcagatgcgg cccggccccc tcgcgcccgc gccggacggc gtcgagcgcg
     8581 agctcatatt ggtccccccg ggtcagggcc gtgatcagcg agcgccacag gccgatattg
     8641 cgcggatcgg cggcgagcgc gcgctcgaaa tcggcggtga agccctcgcg gtcgccgcac
     8701 agccatcgca gccgcatcag cagctccagc ccggcgaccc aaccgggtcg gcccgagagc
     8761 aggcgctcca gagcgacgat cgcggcggcc gggccgcgaa tgtcctcgac cgcggcgacc
     8821 agcccgagca ggatctcccc gtcgtgcggg gcgaggcgcc gggccgcctc gaacagctcc
     8881 accgacggca ggccggcctc caggtgaacg cgcgccagcg tatgggcgat cagggggtcg
     8941 cgcggcgcca gctccgcggc cctcgtcatc atcgcgatcg cgggctcgag atcttccagc
     9001 tggcgatgca gcaggcctgc gacctgccag agccgagcgt cgcccggcgc cgaccggcgc
     9061 gcggcctcga gcagcgccag cccctcgggc tccagccccg ccttcagcgc cttgaggccg
     9121 gcattgtagg cggaggtcga atcggccgga tcgacgcgcg cgatggcgag ggcggtttcg
     9181 ctcacagccc ctccgcgccg gcggcgccgc tccgcggcac gatcgcggcg accgcgcgcc
     9241 gctcggccgc gctgagcgcc caggcccaga cgtcgaagat gaagacgacg cgcaattcgg
     9301 ggctgtcgtt ccacgcttca tgctcgatcg tgtcgtcgaa gacgaaggcg gtgccgggcc
     9361 gccagtcgcg ggtctgggcg ccgacccgaa agccgcagcc ggccggcacg atcagcggca
     9421 ggtggcagac cagtcgggtg ttggccacgc ccgtatgggg cgggatccgg gtgcgcggcg
     9481 cgagcagcga gaacatcgcg ttgggcgagg cgccgggcac gtcgggctgc ggcagccgcc
     9541 cgagcagagc catcgtcctc gggcagtggc gggcgttgcg ttcgatgcgc cggccgttct
     9601 ggaggaggtg gacggcggtc cagtcgcggg agcggttgag cgcctgccat tgccggaccg
     9661 gcacgtcgtc cggatattgg atgtagggga cgagctccgc cgcctcggaa gcgatcaggg
     9721 cgtcgaattc ctcccggatc tcaggcgtgg ccgcttcgag ctcgccgagc caggggaaca
     9781 ggtcgcggtc gtggaactcg accggcggca ggccgggaaa atggaaatgg ctcggctcct
     9841 gcgcgaaggg ccgcgtgatc cgggcgacgt tggtgacgaa gcggtcgatt cgcgcctgct
     9901 cggcgggagt cgtcccgccg ccggccaggc cgccgcgaag gcggccggcg agcgcctcct
     9961 ggaagcgcgc atagctttgc tccgcatggg ccagcatcgg cgcgagcccg ggcgggtcgt
    10021 ccccggccgg ccgctgcgcc agcgcatggc cataggcttc gccggcctcg tccgccttgc
    10081 ccatccgttc cagcagcatc gcccgcacca gcagcgccga gaggtcgacc gggtcgaggg
    10141 ccagcgcccg cgagacggcg gtcatcgccg cgccgagatc gccggtgccc cggcacatgg
    10201 cggcgagctt cagccaggtg cccaggcggt ccggagtctc ggccaccgcg gcctcgagca
    10261 ggcggcgggc gtcgccgagc ctgccggcct gggcggcggc gtcggcctcg gcctcggcgc
    10321 gggaaagctc ggcgtccatc gcccgccttt tgccgggatt cggctcaaat gaaaacgacc
    10381 taccgcttcc gggcgcgcac ctcgacaggg ccggccgcgc gccctaggtc tccttcccgt
    10441 gggcgcaggc acgacaccgg aagaactgct tcggatcgcc tcccagcatc gcgaggccgg
    10501 gcgcgtcgcc gaggcgatcg gcgcctacga ggcggcgctc gcgctccggc ccggcctgcc
    10561 caacagctgg tacaacctcg cccggctcca gcgccaggcc gggcgcttcg aggcggcgct
    10621 cgcttcctat gcgcaggcgc tggcccacgg ggtcagcgag cccgaggagg tccacctcaa
    10681 tcgcggagtc atctatgccg acgatttggc ccgcagcgac gcggccgagg cggagctggc
    10741 caaggcgctc gcgctcaatc cgcgctacgt gccggcgctg ctcaacctcg gcaatctcca
    10801 cgacgaccgc ggcgaccgga cgagcgcccg ctcggcctat gaaagggccc tggcgatcga
    10861 gccggacaac gtcatcgcgc tggcccggct ggcgggggcc gcgccgctgt ccggtccgga
    10921 cgaccccctc ctcggccggc ttcggacggg gatcgcccga gcgggggccg atccggcggc
    10981 gcgggccgac ctcggcttcg cgctcggcgc cgccctggat cggctgggag cctatgacga
    11041 ggcgttcgcc gcctttgccg cggccaatga agccagccgc cagagctcgc cggtccggta
    11101 cgaccgccgc gcgcaggagc ggttcgtcga ccggatgatc gagtccttcc cggagcccgc
    11161 gcggacggcg gcggcgccgg gcggggaggc gccgctcttc atctgcggca tgttccggtc
    11221 gggctcgacc cttgccgagc agatccttgc ccggcacgag gacgtgaccc ccggcggcga
    11281 gctgggcctc gtcaccgctt tggcggcgag gcttcgcccc tatccggagg cggcggcggc
    11341 ggccggtccg gaagcgctgg ccgaatggcg ctccgactat ctggcgcggc tgcgccggat
    11401 ccatcccggc ggcgggctgg tgaccgacaa gaggcccgac aatttcctcc acatcggcct
    11461 gatcaagagc ctgttccccg aggccaggat cgtccacacc cggcgcagcc cgccggacaa
    11521 cgtgctttcg ctctacttcc tgcacctcga tccggccatg gcctacgccc gggacctggg
    11581 cgatgcggcg cactggtacg gccagtatcg ccggctgatg gcgcattggc ggcggctcta
    11641 cggggacgat atccacgacc tcgactacga cgcgctggtc gccgacccga gaccgcatat
    11701 cgaggccctg ctcggcttct gcggcctgtc ctggcgggag gcctgcatgt ccttccacga
    11761 ggccggcaat gcggtgagga cggcgagcgc cgcgcaggtg cgggagcctc tctaccgccg
    11821 ctcttcgggc cgctggcgca attacgagcg gcacctcggc ggcttgatcg aggccctgcg
    11881 ggacgtctga acagcgcccc aaaagaagag ggcgggcctc cgaagagacc cgccccagga
    11941 actcgatgac gcctgttctt agaagtcgag cgtcacgccc gcgaagatgt agcgacccag
    12001 cgagtcgtac acctgcggat aggtgttgcc gttgccgaag ggcgccggag tcaccgcgat
    12061 cggcgggatc cggtcgagga tgttgttggc accgagccgg aagctgtagt gatcgccgat
    12121 acgggcggtc agcaccaggt cgatgtagct ctgcgagccg aagtggcggt cgccctcgtt
    12181 caccgcaccg gcgaggtccg gatccgcgct cgtcaggtcg ttgtcgaccg ccgagaagta
    12241 gcgccactgc gccgacaggc cgatgcccga cgggtgggtg tagctgacgc ggaacttgtg
    12301 cctccactcg gggctcggag tgccgcactg gccgccgaag aagccggcgc agttgaactt
    12361 gatgtccgcc agcggattga cgatcagctc gtccagccag gtgccgacga agctgaacgc
    12421 gacgctgccc atcccgccga gcggctgggt gtaggaagcg ttgacgtcca cgccccgggt
    12481 cgagagaccg cccacgttcg tgttggtgtc gaccgtgaac ccgttcggag tcagccacag
    12541 cgagccggcc gcatcgcggt tgatcttgcc gcagaagaac ggatcgccgg tggcgacgca
    12601 ctgcgccagg atcgtgtcgg cgccgatgat cccgatcgcg ttgtcgagcc gaatgtcgaa
    12661 atagtcgacg gtcagcgcga agcgcggaag gaagcgcggc tgcaacacga cgccgagcgt
    12721 gatcgagtcg gcggtttccg gagtcaggtt cgggttgcct ccgagaaggc tgttatactg
    12781 gcccgccgga ttgatcggaa ccttgccgta ctgggccgcc ggaaggtcgg cgagcacgca
    12841 ctgggcctgg gtcgccgccg gggtcggacc cgcgcacgga tcggtgctgc cgctgaggcc
    12901 caccgcctga gccgagaaca gctcgacgat gttcggagcg cgcaccgccc ggttgtagct
    12961 gccgcggaag cggatgtcgc gaaccggagc ccactcgccc tcgaacttgt aggtgtcggt
    13021 cgagaaggaa ttgttggcga cgtcgtactt cgaatagcgg tagccggcgc ggaactcgag
    13081 ctgctcgaag aagggccggt cggagatcag cgggatctgg agctccgtga agagctcctt
    13141 cacgtcgaac tcgcccgtca cgccgatcgt ggcgccgccc tggccggcga ggtcgccggt
    13201 cgagaaggcc tggtcggtgc tgaactccag ccgctccttg cgatactcgg cgccgacgtt
    13261 gaccgccacg ccgctctcgg accaggggct ctggacgcca tgctcgccga ggtcggcggt
    13321 gatcgatccg gtcgccaccg tctcctgcgt gttgccgcgc gagaagcccg gcgtctgcag
    13381 atagtcgagg gccgcctgcg tcacgccgcc gagcgcgaag atgtcgtagg ggacgcagtt
    13441 cgcatcgatg ccggtgagca ccgagcggca gaccggagtt ccgttcggac ccgtgacgac
    13501 gtcgagggcg cggccgatgc gagtgaccga gaagtcgttg aagtaggtct gcgcgaagac
    13561 cacgcggcca tactggtagg aggcatcgta ggagaaaccg ttgcccaggt cgcc
//
LOCUS       DASVQZ010000128        11623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_127053, whole genome shotgun sequence.
ACCESSION   DASVQZ010000128 DASVQZ010000000
VERSION     DASVQZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11623
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127053"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VF547_03605"
     CDS             <1..635
                     /locus_tag="VF547_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8261942.1"
                     /translation="GKSTLSRLLFRFYEPTSGRIAIDGQDICDVTQASLRAALGIVPQ
                     DTVLFNDSIGYNIGYGRDGATQAEIEAAARGAAIHDFILSLPDGYDSQVGERGLKLSG
                     GEKQRVAIARTLLKDPPILILDEATSALDSRTEAAIQETLQSVTARRTSIVIAHRLST
                     VVDADQIVVLDGGRVAERGTHAQLLRKSGLYADMWARQQSEAEEKAEAAE"
     gene            825..2585
                     /gene="recQ"
                     /locus_tag="VF547_03610"
     CDS             825..2585
                     /gene="recQ"
                     /locus_tag="VF547_03610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX8261943.1"
                     /translation="MTPLSILNETFGFPAFRGVQEQVIGRVMAGRHTLAVMPTGSGKS
                     LCYQVPALARPGTALVISPLIALMHDQIRSASSFGIRAASLTSADEDRQETLARLKRG
                     ELDLLYVAPERASGSGFRGLAERTPLSLIAVDEAHCVSEWGHDFRPDYRLLRPLLDAL
                     PNVPRLALTATADRQTRADILVQLGIPEDGLIVAGFDRPNIRYHVRPRDGVGQQLKAL
                     LADQPGPGIVYAPSRDKTEKLAAQLAEGGRKVLPYHAGLDPRVRSANQQAFVASEDMV
                     MVATIAFGMGIDKPDVRFVAHAGLPKSIEAFYQETGRAGRDGDPAEAWLFWGAEDFAR
                     ARQRLETEVPEARRPGERLRLNALAAMVETAGCRRAMLLRHFGEDPPEGCGNCDNCLA
                     PPVTVDATEAARKLLSAAFRTDMRFGIQHLTDVLAGRDTEKVRSFGHHRVSVFGIAGE
                     EELALIKPVARALLARDALRADPYGGLSFGPAAKPILRGEETLELVLPPARRPRRRRE
                     RTEFPHDPLFEALRARRRDLARDAGVPPYVIFHDSTLREMAEIRPETLSALSRVSGVG
                     QAKLERYGEAFVEVVRGFAS"
     gene            2582..2983
                     /locus_tag="VF547_03615"
     CDS             2582..2983
                     /locus_tag="VF547_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEX8261944.1"
                     /translation="MRRLDDSTFVAWQIGAADVPAAAARGVRLIVNNRPDREEPGQPH
                     SAEIEAAARAAGLGYRHIPVAKGFSAWQVEAMADALEHGPVLAFCRSGMRSTHLWALA
                     RASRGIPAPTLIGLAADAGYDLSDLADWLSR"
     gene            complement(2973..3170)
                     /locus_tag="VF547_03620"
     CDS             complement(2973..3170)
                     /locus_tag="VF547_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX8261945.1"
                     /translation="MALVEAARFYNSVEGSLARARLAAEGIESVLFDTEMNWGGLDGV
                     VPVRLMVDEDDYLLARRCLSD"
     gene            complement(3170..3703)
                     /locus_tag="VF547_03625"
     CDS             complement(3170..3703)
                     /locus_tag="VF547_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX8261946.1"
                     /translation="MTWRDEVLAFWFGLAKEQWWKADPALDAEIRDRFLALWEEKRAN
                     VPETFLGSARDALAAVILFDQFPRNMMRGHADQFSTDPLALAIAKGAVDRKLDDAMSP
                     AERGFLYMPFQHSEDLGDQKRSVALFTALGDPYQLGYARKHHDVIERFGRFPHRNSIL
                     GRPPRPAEIEAGEVVPW"
     gene            complement(3819..5246)
                     /gene="tldD"
                     /locus_tag="VF547_03630"
     CDS             complement(3819..5246)
                     /gene="tldD"
                     /locus_tag="VF547_03630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX8261947.1"
                     /translation="MTYTDPRRFLYRSDALTPDAALGLTAEALKGCDDGELYLQYTAA
                     ESFGFDDGRLKTADFNTSAGFGLRGVSGETTAFAHANELSEAAIRRAAETMAVLDPAR
                     GARPVPPRRTNATLYTDADPLSLVPFAKKVALCQQVDAAARARDPRVVQVAVSLAGSW
                     SVIEIVRPDGFVAHDVRPLVRLNVQVVVEQNGRRETGYHGQGGRFLYDDLFQEAVWNR
                     AIDVALAQALVNLESVAAPAGEMTVVLGPGWPGVLLHEAIGHGLEGDFNRKGTSAFSG
                     RIGERVAAPGVTVVDDGSISERRGSLSIDDEGTPTQCTTLIEDGILKGYIHDRLNARL
                     MGVEPTGNGRRENFAHAPMPRMTNTFMLGGKDDPGEILARAKNGIYAKSFGGGQVDIT
                     SGKFVFSCTEAYRIENGRLGAPVKGATLIGDGPTVLTRVAAIGNDMALDEGVGVCGKG
                     GQSVPAGVGQPTLLIDGLTVGGTAA"
     gene            complement(5310..5822)
                     /locus_tag="VF547_03635"
     CDS             complement(5310..5822)
                     /locus_tag="VF547_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261948.1"
                     /translation="MGLFDWLTGHKPAPAGVKRRSEAKLREALMGVNRKSAPFKIRDG
                     AKEGVDLVAEWRIVDAKWTEVFAQAGIKRVFKVLMKFDAAKGEVRAVDQEWEVEWRAG
                     TPELFLKGSAFRGRKWEKSFETVYAFREDGSFGEIYDYRFDTSEIKDPLIEAAHKAGW
                     GWRGVAFAKL"
     gene            complement(5878..6381)
                     /locus_tag="VF547_03640"
     CDS             complement(5878..6381)
                     /locus_tag="VF547_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4087 domain-containing protein"
                     /protein_id="HEX8261949.1"
                     /translation="MSRSASFAAALAAFLAACSPEAGPPAEGNGEAALAAPSGPANAI
                     QNAPAGGDGERPGNQVAAKAPSPAAGERRCGWLHNPTPGNWWLDDRDGQWVLASQGSE
                     PAPGMDDMPDMSTVGWVETNGHYGHGCACMTIAHDPATKRVTRIADAQPKPLKQCRAD
                     KSLPRPD"
     gene            complement(6383..6763)
                     /locus_tag="VF547_03645"
     CDS             complement(6383..6763)
                     /locus_tag="VF547_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261950.1"
                     /translation="MLSFGTPQKEAVEALTKAFGGPPTEQDTNSECGGGGLEFAEWEG
                     RLTAWFEEGRFAGWDDKGGLKTVGGVGVGSTRAQLATLRGLEVEESTLGTEFRAGGLS
                     GILDSKAPNAKVTHLWGGATCVFR"
     gene            complement(7062..7271)
                     /locus_tag="VF547_03650"
     CDS             complement(7062..7271)
                     /locus_tag="VF547_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX8261951.1"
                     /translation="MQTDRFTPEISFVTTPRVACDGASETHPALGHPRVYLQIDEKGF
                     VDCGYCDKRFVLKGGPADEGGAEAA"
     gene            complement(7441..8343)
                     /locus_tag="VF547_03655"
     CDS             complement(7441..8343)
                     /locus_tag="VF547_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8261952.1"
                     /translation="MSAFATKPVALATGVTLNVATAGPEDGEPILFLHGFPESHRTWR
                     HQLVGLADRYRVIAPDQRGFGASDKPEGVENYETDRIIADVTALADALGIDRFTLAGH
                     DWGGAVAWAAALLHPFRVERLVIVNAPHPLVFQKSLIEDEAQREASQYISFFRSPAAE
                     AAITGMGLETFLERGLLRHADATKLPDEERRHYLDDWSQPGALTAMLNWYRASKMVVP
                     QPGESAKAPLWTHAPFPKLKMPTLVVWGLKDTALLPVQIEGLNDVVEDLRLVTSPSAG
                     HFITWEEPGTVTSAIADFMAETAS"
     gene            complement(8340..8924)
                     /locus_tag="VF547_03660"
     CDS             complement(8340..8924)
                     /locus_tag="VF547_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX8261953.1"
                     /translation="MSDAPSPIDSAEEAPAPMSDASTLCVGCGLCCSGVLFTNARAEP
                     DEVPRLKALGLEVEQGRGERLQFRLPCPHHDDGHCGIYADRFLKCRSFRCALLKRLDA
                     GEVALGEAQAVVSQAKAMLARVTQLDPAAAQFRVRTAQRAEPAAAEAGEDAARRRRLL
                     IESLALDLFFDRRFRNRAAVRMDRSRGKSEGEEG"
     gene            complement(8921..9820)
                     /locus_tag="VF547_03665"
     CDS             complement(8921..9820)
                     /locus_tag="VF547_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8261954.1"
                     /translation="MTDLILQTLYPATHDLGGFKVHRTLPHRERTAVGPFIFFDQMGP
                     AKLEPGGGIDVRPHPHINLATVTYLFAGAIDHRDSLGTFATIEPGAVNLMTAGRGIAH
                     SERSPAAVRPGGPELSGIQTWLALPRSKEEIDPAFEHVPKDRLPLVEAGAAAARVVMG
                     TLWGAASPVTCHSETIYADIQLGRGGAMPVDAGADERAVYVAEGEASLDGLALERSVL
                     YVLAPGAAATLRSQTGAHVMLCGGAPLDGPRHVWWNFVSSSRERIQQAKEDWRAGRFV
                     LPPDDRDEFIPLPVAPPKTVSYP"
     gene            complement(9866..10864)
                     /locus_tag="VF547_03670"
     CDS             complement(9866..10864)
                     /locus_tag="VF547_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HEX8261955.1"
                     /translation="MTELTKFETREAGEEENVTRIIEILRAQQLKTYKPGNTLRDAHT
                     KRIGLVEAEFTVEPDLPEALRVGLFAEPRRYGAWVRFSSASEKPRSDAIKDIRGAAIK
                     LRDVPGERIPESDEPTTQDFLMVSAPTMPLGTVRLFRTAVQLINQYSPLLFAALLLLT
                     GRFRVLKTLNAMNINPTSPVDIRYWSTTPYLFGPDRAAKYSIVPTSSYVSRLPDKPGD
                     TYLSDNLEQHLSQADATFDFMVQLRTDPETMPIENAAVEWREEEAPFVKVATLRIPQQ
                     RFRTPERDAMAAALSFSPGHALVEHRPIGGVNRARMRVYREQADFRQKRDGVARRT"
     gene            complement(10951..11235)
                     /locus_tag="VF547_03675"
     CDS             complement(10951..11235)
                     /locus_tag="VF547_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX8261956.1"
                     /translation="MSLPFTGPLALEIEQRLRAALAPAHLVVTNDSARHRGHAGDDGS
                     GESHFTVRIESERFAGMSRLERQRAVNRVLADLLRDRIHALAISASAPGE"
     gene            11274..>11623
                     /locus_tag="VF547_03680"
     CDS             11274..>11623
                     /locus_tag="VF547_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8261957.1"
                     /translation="MASAGSRQTRFHGRAEARGICGHPGCDAPGEFRAPAGRGPSFDG
                     PGEWQWLCLEHVREFNAGYNYFEGMSSDEIQAAHNPYGGWDRETRAFARMGADGPPKW
                     ADFQDPIDAIGARFR"
BASE COUNT         1701 a         4089 c         4066 g         1767 t
ORIGIN      
        1 ccggcaagtc gaccttgtcg cggctgctgt tccgcttcta cgagcccacc tccgggcgca
       61 tcgcgatcga cggacaggac atttgcgacg tgacgcaggc gagcctcagg gctgcgctcg
      121 gcatcgtccc gcaggacacg gtgctgttca acgactcgat cggctacaat atcggctacg
      181 gccgcgacgg cgccacccag gcggagatcg aggccgccgc ccgcggcgcg gcgatccacg
      241 acttcatcct gagcctgccc gacggctacg actcgcaggt gggcgagcgc gggctgaagc
      301 tgtcgggcgg cgagaagcag cgcgtcgcga tcgcccgcac gctcctcaag gacccgccga
      361 tcctgatcct cgacgaggcg accagcgcgc tcgacagccg caccgaggcg gcgatccagg
      421 agacccttca gagcgtgacg gcgcggcgca cctcgatcgt catcgcccac cgcctctcga
      481 ccgtcgtcga cgccgaccag atcgtcgtgc tcgacggggg gcgggtcgcc gagcgcggca
      541 cccacgcgca attgctgagg aagagcggcc tctacgccga catgtgggca aggcagcaga
      601 gcgaggcgga ggagaaggcc gaggcggcgg aataggggct cgctcggcga tcattggctc
      661 cgcgccggcc tgaacttgtt tcagggtcca gttctccgcc cgcgaggcgg ttcttccctg
      721 gatcctgaat caggtcggac gacgaaaaga accggatatc caatgttcta gtcaacgcct
      781 cgccgctctc gacagggcct tcccccatcc gctagcagcg gctcgtgacc cccctttcca
      841 ttctcaatga gacattcggc ttcccggcct tccgcggagt ccaagagcag gtgatcggcc
      901 gggtcatggc cggccggcac acgctcgccg tcatgccgac cggctcgggc aagtcgcttt
      961 gctaccaggt gccggccttg gcgcggcccg gaacggcgct cgttatctcg ccgctgatcg
     1021 cgctgatgca cgaccagatc cgctccgcct cgagcttcgg catccgagcc gcttcgctca
     1081 cctcggcgga cgaggaccgc caggagacgc tggcaaggct gaagcgcggc gagctcgacc
     1141 ttctctacgt ggcgccggag cgggcgagcg gaagcggctt tcgggggttg gcggagcgga
     1201 cgccgctgtc gctgatcgcg gtcgacgagg cgcattgcgt gtccgaatgg ggccacgact
     1261 tccgacccga ctaccggctg cttcggccgc tgctcgacgc cctcccgaac gtgccccgcc
     1321 tcgcgctgac cgccaccgcc gaccgccaga cccgcgccga catcctcgtc cagctcggga
     1381 tccccgagga cgggctgatc gtcgccggct tcgaccggcc caacatacgc taccacgtcc
     1441 gcccgcgcga cggcgtcggc cagcagctca aggcgctgct cgccgatcag ccggggccgg
     1501 gcatcgtcta cgcgcccagc cgcgacaaga ccgagaagct cgccgcccag ctcgccgagg
     1561 gcggccgcaa ggtcctgccc tatcatgccg ggctcgatcc gagggtgcgc tcggccaatc
     1621 agcaggcctt cgtcgcctcc gaggacatgg tgatggtcgc gaccatcgcc ttcggaatgg
     1681 gcatcgacaa gccggacgtg cgcttcgtcg cccatgccgg cctgcccaag tcgatcgagg
     1741 ccttctacca ggagaccggc cgcgccggcc gcgacggcga tcccgccgag gcctggctgt
     1801 tctggggcgc cgaggatttc gcccgcgccc gccagcggct cgagaccgaa gtgccggagg
     1861 cgcgccggcc gggcgagagg ctgcggctca acgcgctcgc cgcgatggtg gagacggccg
     1921 ggtgccgccg cgccatgctg cttcgccatt tcggcgaaga cccgccggaa ggctgcggca
     1981 attgcgacaa ttgcctggcg ccgccggtca cggtcgacgc caccgaggcg gcgaggaagc
     2041 tgctttcggc cgccttccgg accgatatgc gcttcgggat ccagcatctg accgacgtcc
     2101 tcgccgggcg cgacaccgag aaggtccgaa gcttcggcca tcaccgggtg tcggtgttcg
     2161 ggatcgccgg cgaggaggag ctggcgctga tcaagccggt cgcccgcgcg ctgctcgccc
     2221 gcgacgcgct tcgcgccgac ccctatggcg gcctgagctt cggcccggcg gccaagccga
     2281 tcctgagggg cgaggagacg ctcgagctgg tcctgccccc cgcccgcagg ccccgccgcc
     2341 gccgcgagcg gacagagttc ccgcacgatc cgctgttcga ggcgctgcgc gcccgccgcc
     2401 gcgacctcgc ccgcgacgcc ggcgtcccgc cctacgtcat cttccacgac agcaccctgc
     2461 gcgagatggc cgagatccgg cccgagacgc tgagcgcgct gtcgcgagtc tccggcgtcg
     2521 gccaggcgaa gctcgagcgc tatggcgaag cgttcgtcga agtcgtgcgt ggatttgcgt
     2581 cgtgaggcga ctcgacgact cgaccttcgt cgcctggcag atcggcgccg ccgatgtgcc
     2641 ggccgccgcc gcccggggcg tgcgcctcat cgtcaacaac cgtcccgacc gcgaggagcc
     2701 gggccagccg cactcggccg agatcgaggc cgccgcccgc gccgccggcc tgggctatcg
     2761 gcacatcccg gtggccaagg gcttttccgc ctggcaggtc gaggcgatgg ccgacgcgct
     2821 cgagcacggc ccggtgctcg ccttctgccg gtccggcatg cggtcgaccc atttgtgggc
     2881 actggcgagg gcgtcgcggg ggattccggc gcccaccctg atcggcctgg ccgcggatgc
     2941 cggctacgac ctgagcgatc tcgccgactg gctcagtcgc tgaggcaccg gcgggcgagc
     3001 agatagtcgt cctcgtccac catcagccgc accgggacca ctccgtcgag cccgccccag
     3061 ttcatctcgg tgtcgaacag gacgctttct atcccctccg cggcgaggcg cgcgcgggcc
     3121 aggcttccct cgacgctgtt gtagaagcgg gcggcctcga ccagcgccat caccagggaa
     3181 ccacttcgcc ggcctcgatc tctgcgggac gcggcggtcg gccgaggatc gaattgcgat
     3241 ggggaaagcg gccgaaccgc tcgatgacgt catggtgctt gcgggcgtag ccgagctgat
     3301 aggggtcgcc gagcgcggtg aacagggcga cggagcgctt ctggtcgccc aggtcctcgc
     3361 tgtgctggaa gggcatgtag aggaagccgc gctccgccgg gctcatcgcg tcgtcgagct
     3421 tccggtccac cgcgcccttc gcgatcgcca gcgcgagcgg gtcggtcgag aactggtcgg
     3481 catggccgcg catcatgttg cgcgggaact ggtcgaacag gatcaccgcc gccagcgcgt
     3541 cgcgggcgct gcccaggaag gtctcgggca cattcgccct tttctcctcc cacagggcga
     3601 ggaagcggtc gcgtatctcg gcgtcgagcg ccgggtcggc cttccaccat tgctccttgg
     3661 caaggccgaa ccagaaggcg agaacctcgt cccgccaggt caccggataa gcccgtcatt
     3721 gcgaggagcg aagcgacgaa gcagtccgac tgcggcgttc gcgaagaacg ccgcactgga
     3781 ttgcctcgct tcgctcgcaa tgacgggaag gcgggccgtc acgccgcggt gccgcccacc
     3841 gtcaggccgt cgatcagaag cgtcggctgg ccgactccgg ccggcaccga ctggccgccc
     3901 ttgccgcaga cccccacgcc ctcgtcgagc gccatgtcgt tgccgatcgc cgcgacccgg
     3961 gtcagcaccg tcggcccgtc gccgatcagc gtggcgccct tgaccggcgc tccgagcctg
     4021 ccgttctcga tccgataggc ctcggtgcag gagaagacga acttgccgct ggtgatgtcg
     4081 acctggccgc cgccgaagct cttcgcatag atgccgttct tcgcccgggc gaggatctcg
     4141 cccggatcgt ccttgccgcc gagcatgaag gtgttggtca tgcgcggcat cggcgcgtgg
     4201 gcgaagttct cgcggcggcc gttgccggtt ggctcgaccc ccatcagccg ggcgttcagc
     4261 cggtcgtgga tatagccctt gaggatgccg tcctcgatca gggtggtgca ctgggtcggg
     4321 gtgccctcgt cgtcgatgga cagcgagccg cgccgctcgg agatcgagcc gtcgtcgacc
     4381 accgtcaccc cgggggccgc cacccgttcg ccgatccgcc cggagaaggc ggacgtgccc
     4441 ttgcggttga agtcgccctc gaggccgtgc ccgatcgcct cgtggaggag gactcccggc
     4501 cagcccggcc caagcaccac cgtcatctcg ccggcgggcg ccgcgaccga ttcgagattg
     4561 accagcgcct gggccagggc gacgtcgatc gcccggttcc acaccgcctc ctggaacaga
     4621 tcgtcgtaga ggaagcggcc gccctggccg tgatagccgg tctcgcgccg gccgttctgc
     4681 tcgacgacga cctggacgtt gagccggacc agcggtcgca cgtcgtgggc gacgaagccg
     4741 tccggccgga cgatctcgat caccgaccag cttccggcga gcgaaacggc gacctggacg
     4801 actcgcgggt cgcgcgcccg cgccgcggcg tcgacctgct ggcacagcgc caccttcttc
     4861 gcgaacggga cgaggctgag cggatcggcg tccgtataga gcgtcgcgtt ggtccgccgg
     4921 ggcggcaccg gccgcgcgcc gcgcgccgga tcgagcaccg ccatcgtctc ggcagcgcgg
     4981 cggatcgcgg cttccgaaag ctcgttggca tgggcgaagg ccgtcgtctc gccggacacg
     5041 ccgcgcagcc cgaagccggc cgacgtgttg aaatccgccg tcttcaggcg cccgtcgtcg
     5101 aagccgaagc tctccgcggc cgtatattgg aggtaaagct cgccgtcgtc gcagcccttg
     5161 agcgcctcgg cggtgaggcc gagcgcggcg tcgggcgtga gggcatcgct gcgatagagg
     5221 aagcggcggg ggtcggtata ggtcatggac cggatatagg ggggcgcatc tctcccgtca
     5281 tcccagcgca ggccggggcg acgtcgcgcc tacagcttcg cgaaggcgac gccccgccag
     5341 ccccagccgg ccttgtgcgc ggcctcgatc agcggatcct tgatctcgct cgtatcgaac
     5401 cgatagtcgt agatctcgcc gaacgaaccg tcctcgcgga acgcatagac cgtctcgaag
     5461 ctcttctccc atttgcgccc tcggaaggcg gaccccttga ggaagagctc aggcgtgccg
     5521 gcgcgccatt cgacctccca ttcctggtcg accgcgcgca cctcgccctt ggccgcgtcg
     5581 aacttcatca gcaccttgaa cacgcgcttg atgcccgcct gggcgaagac ctcggtccat
     5641 ttcgcgtcga cgatccgcca ctcggcgacg aggtcgacgc cctccttcgc gccgtcgcgg
     5701 atcttgaacg gggcgctctt gcggttgacg cccatcagcg cctcgcgcag cttcgcctcg
     5761 gagcgccgct tgacgccggc gggcgcgggc ttgtggccgg tcagccagtc gaacaatccc
     5821 attggccatc tcctcgtcat cccagggcag gcccggaccg aagcgtgatg caggcggcta
     5881 gtcgggccga ggcaggctct tgtcggcgcg gcactgcttc agcggcttgg gctgcgcgtc
     5941 ggcgatccga gtcacccgct tcgtcgccgg atcgtgggcg atcgtcatgc aggcgcagcc
     6001 gtggccgtaa tggccgttgg tctcgaccca gccgacggtg ctcatatccg gcatgtcgtc
     6061 cattcctggc gccggctcgc tgccctgcga ggccaggacc cactggccgt cgcggtcgtc
     6121 cagccaccaa ttgccgggcg tcgggttgtg cagccagccg caacggcgct cgccggcggc
     6181 cgggctgggc gccttggccg cgacctgatt gcccggacgc tcgccgtcac cgccggcggg
     6241 ggcgttctgg atcgcgtttg ccggtccgga cggcgcggcg agcgccgctt cgccattgcc
     6301 ttcggccggc gggccggcct ccggcgagca ggcggcgagg aaagccgcga gcgcggcggc
     6361 gaaggacgct gagcggctca cctcaccgga acacgcacgt cgcgccgccc cacaaatggg
     6421 tgaccttggc attcggcgcc ttcgaatcga ggatgccgct gaggccgccg gccctgaatt
     6481 cggtgccgag cgtgctctcc tccacctcga gcccgcgcaa ggtcgccagc tgcgcgcggg
     6541 tcgagcccac gccgacgccg ccgacggtct tgagcccgcc cttgtcgtcc caaccggcga
     6601 atcgtccctc ctcgaaccag gcggtgagcc ggccttccca ctcggcgaat tcgaggccgc
     6661 cgccgccgca ttcggaattg gtgtcctgct cggtcggtgg cccgccaaag gccttggtga
     6721 gcgcctcgac cgcctccttc tgcggggtgc cgaacgaaag catctcagtc cgcgagagcc
     6781 gcaggccatg accttcgagc accaccgggg cgggctcgga ctgggccccg gcacgctccg
     6841 acgcagccgc gttcgaaccg gccggctcct ccgccgggac ggaggcattg tccccaccgg
     6901 acggtcccga ctccggcccg caggccgcga gcgtcgctac gagcagtggc gccaaggccg
     6961 gaccgaatga gagcttgagc aaaaccattc cccttgccgc cagcctcccg cttggacctt
     7021 gtcgcgggac atggcaacgg gcgaccgtcg aaagccgacg ctcaggccgc ctcggcgccg
     7081 ccctcgtcgg caggaccgcc cttcagcacg aaccgcttgt cgcaatagcc gcagtcgacg
     7141 aaccccttct cgtcgatctg cagatagacg cggggatggc cgagcgccgg gtgagtctcg
     7201 ctcgccccgt cgcaggcgac gcgcggcgtc gtcacgaagc tgatctcggg ggtgaagcgg
     7261 tccgtctgca tggccggggc gtgtagcaac cgggtggagg ggacggaagg aaaatcctcc
     7321 cccggatttc ggggggaaag gggaccgcgc ggagcgcggt ggaggggttc ttcgaagtca
     7381 gcgaggccgc tccaccatgc ttcgcatggt ccccctcccc tgcaaatgca cgggaggaat
     7441 ctaggaggcc gtttcggcca tgaaatcggc gatcgccgag gtcaccgtcc ccggctcttc
     7501 ccaggtgatg aagtggcccg ccgagggcga ggtcacgagc cgaaggtcct cgaccacgtc
     7561 gttcagtccc tcgatctgga ccggcagcag ggcagtgtcc ttgaggcccc acacgaccag
     7621 cgtcggcatc ttcagcttcg ggaagggcgc atgggtccat agcggcgcct tcgccgactc
     7681 tcccggctgc ggcaccacca tcttcgaggc gcggtaccag ttgagcatgg cggtgagggc
     7741 gcccggctga gaccagtcgt cgagataatg ccggcgctcc tcatcgggca gcttggtcgc
     7801 gtccgcatgg cggagaagcc cgcgctcgag gaaggtctcc agccccatgc cggtgatcgc
     7861 cgcctcggcg gccggcgagc ggaagaagct tatatattgc gacgcctcgc gctgcgcctc
     7921 gtcctcgatc aggctcttct ggaagacaag gggatggggg gcgttgacga tgacgaggcg
     7981 ctccacccgg aacgggtgca gcagcgccgc ggcccaggcg acggccccgc cccaatcgtg
     8041 gccggccagg gtgaagcggt cgatcccgag cgcgtcggcg agggcggtga cgtcggcgat
     8101 gatccggtcg gtctcgtaat tctcgacgcc ctccggcttg tcggacgcgc cgaagccgcg
     8161 ctggtcgggc gcgatgaccc gatagcggtc ggcgagcccg accagctggt ggcgccaggt
     8221 gcgatgcgat tcgggaaagc cgtggaggaa caggatcggc tcgccgtcct ccggcccggc
     8281 cgtcgcgacg ttgagggtca ccccggtagc cagtgcgacc ggcttcgtgg cgaatgcgct
     8341 catccttcct ccccttccga cttgccgcgc gaccggtcca tccggaccgc cgcgcggttg
     8401 cggaaccgcc ggtcgaagaa caggtccagc gccagcgatt cgatcagcag tcggcggcgc
     8461 cgggcggcgt cctcgccggc ctcggcggcc gcgggctcgg cgcgctgggc ggtgcggacg
     8521 cggaactggg cggcggccgg atcgagctgg gtcacccggg cgagcatcgc cttggcctgg
     8581 ctgaccaccg cctgcgcttc gcccagcgcc acctcgccgg cgtcgagccg cttcaggagc
     8641 gcgcagcgga agctgcggca cttgaggaag cggtcggcgt agatgccgca atggccgtcg
     8701 tcgtgatgcg ggcagggcag ccggaactgg agccgctcgc cgcgcccctg ctccacctcc
     8761 aggcccagcg ccttcagcct cggcacctcg tccggctcgg ctcgggcgtt ggtgaacagc
     8821 acgccgctgc agcacaggcc gcagccgacg cacagggtcg aggcgtccga catcggggcc
     8881 ggggcttcct cggcgctgtc gatgggggac ggcgcgtcgc tcacgggtag ctgacggtct
     8941 tcggcggggc gaccggcagc gggatgaact cgtcgcggtc gtccggcggc aggacgaagc
     9001 ggccggcccg ccagtcctcc ttggcctgct ggatgcgctc ccggctcgac gagacgaagt
     9061 tccaccagac gtggcggggt ccgtcgagcg gcgctccgcc gcagagcatg acgtgggcgc
     9121 cggtttgcga gcgcaaggtg gccgccgcgc ccggcgcgag cacatagagg acgctgcgct
     9181 cgagcgcgag gccgtcgagg ctggcttccc cctcggcgac atagaccgcg cgctcgtccg
     9241 cgccggcgtc gaccggcatg gcaccgcctc ggccgagctg gatgtcggcg tagatcgtct
     9301 cgctgtggca ggtgaccggc gaggcggcgc cccacagggt tcccatcacc acccgagccg
     9361 ccgccgcgcc cgcctcgacc agcggcagcc ggtccttcgg cacatgctcg aaggcgggat
     9421 cgatctcctc cttcgagcgg ggcagggcca gccaggtctg gatgccgctg agctccggcc
     9481 cgcccggccg gaccgccgcc gggctgcgct cggaatgggc gatgccgcgc cccgcggtca
     9541 tcaggttgac cgcgcccggc tcgatggtcg cgaaggtgcc gagcgaatcg cgatggtcga
     9601 tcgcgccggc gaacagatag gtgacggtgg ccagattgat gtgcggatgc ggccggacgt
     9661 cgatgccgcc ccccggctcg agcttggccg gccccatctg gtcgaagaag atgaacgggc
     9721 ccaccgcggt ccgctcccgg tgcggcaagg tgcgatggac cttgaacccg ccgagatcat
     9781 gggtggccgg ataaagcgtc tggaggatga ggtcggtcat tcggacctct tatccgtcat
     9841 tgcgagcgaa gcgaagcaag gacgatcagg tgcgcctcgc caccccgtcg cgcttttgcc
     9901 ggaagtcggc ctgctcgcga tagacccgca tccgcgcccg gttgacgccg ccgatcggcc
     9961 ggtgctcgac gagcgcatgg ccgggcgaga aggacagggc ggcggccatg gcgtcgcgct
    10021 ccggcgtccg gaaccgctgc tgcgggattc gaagcgtggc caccttgacg aacggcgcct
    10081 cctcctcgcg ccattcgacg gcggcgttct cgatcggcat cgtctccgga tcggtgcgaa
    10141 gctggaccat gaagtcgaaa gtggcgtccg cctggctgag atgctgctcg agattgtcgg
    10201 acagataggt gtctcccggc ttgtccggca gccggctcac ataggaggag gtcggaacga
    10261 tcgaatattt ggccgcccgg tccgggccga acagataggg cgtggtgctc cagtagcgga
    10321 tgtcgaccgg cgaggtcgga ttgatgttca tcgcgttgag cgtcttgagc acccggaagc
    10381 ggccggtgag caggagcagg gcggcgaaca gaagcggcga atattggttg atcagctgga
    10441 cggcggtgcg gaacaggcga actgtgccca gcggcatggt cggggcgctg accatcagga
    10501 agtcctgggt ggtcggctcg tccgattccg ggatccgctc gccgggaacg tcgcgcagct
    10561 tgatcgcggc gccgcgaatg tccttgatcg cgtcggagcg cggcttctcg ctggcgctgg
    10621 agaagcgcac ccacgcgccg tagcgccgcg gctcggcgaa cagcccgacc ctcagcgcct
    10681 cgggcaggtc cggctcgacg gtgaactccg cctcgaccag gccgatgcgc ttggtatggg
    10741 cgtcgcgcag ggtgttgccc ggcttgtagg tcttgagctg ctgggcacgc agtatctcga
    10801 tgattcgggt gacattctcc tcctcccccg cctcgcgagt ctcgaatttg gtgagctccg
    10861 tcatcgtctg ttccccacgc cccgattccg ggcgatatta gcgcgccgcc gcgtccatgc
    10921 gcgcaaaatc tgatccgatc cgcgcggagt tcattctccg ggcgcgctgg cgctgatcgc
    10981 cagggcgtgg atccggtcgc gcagcaggtc ggccagcacg cggttgaccg cccgctgccg
    11041 ctcgagccgg ctcatcccgg cgaaacgctc cgactcgatc cggacggtga aatggctctc
    11101 gccgctgccg tcgtcgccgg catggccgcg gtggcgggcg ctgtcgttgg tcacgacgag
    11161 atgtgccgga gcgagggcgg cgcgcaggcg ttgttcgatc tcgagcgcga ggggaccggt
    11221 gaaaggaagg ctcatcgcgc ctatatagac ggtttgacgg caaaggtgag agcgtggcga
    11281 gcgcggggag caggcagacg aggtttcacg gacgggcgga ggcgcgcggc atctgcgggc
    11341 atccgggctg cgacgcgccc ggggagttcc gggcgcccgc gggccgcggc cccagcttcg
    11401 acggcccggg cgaatggcag tggctgtgcc tcgagcatgt gcgcgagttc aacgccggct
    11461 acaattattt cgagggcatg agcagcgacg agatccaggc cgcccacaat ccctacggcg
    11521 gctgggaccg cgagacccgg gccttcgccc ggatgggcgc cgacgggccg cccaaatggg
    11581 ccgatttcca ggatccgatc gacgccatcg gcgcccgctt ccg
//
LOCUS       DASVQZ010000129         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_702642, whole genome shotgun sequence.
ACCESSION   DASVQZ010000129 DASVQZ010000000
VERSION     DASVQZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702642"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1666
                     /locus_tag="VF547_03685"
     CDS             <1..>1666
                     /locus_tag="VF547_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8261958.1"
                     /translation="SDPLDQPPLDPSAPLDPLPDLGVDWPDMEQGTDEGIADAPDAGI
                     ADAAAERRYTIRIEGLDADLEAAVKAQFDPLSTLEQNRREPANAAQIDRRAREDADLL
                     AELLRARGFYDALVRTRVEAAPAGGEVAVTLETEPGPLYRFAEVELPGLDGAGGDSEA
                     LRDAFGIAAGQPVDAGRVTAGEAALRVELGRRGYAFAEVGAMDIALDHETRTATLTLP
                     VNPNGARQFGRILVEGRPLFSAKHIQTIARWKPGAAYAAPLVEDLRRALIATGLVSAV
                     AIRPVAGQGPGTVDLAVSLERAPMRTIAGQAGYGTGEGIRVEASWQHRNLLAPEGAVT
                     FRGVLGTQEQLIGAVLRRSNFLHRDQVLNAQVAISHIDRAAYDARTSLLAGSIERQTN
                     LIWQKKWTWSAGAELIATDERDVDLASGETRRRTFFIGALPASLSYDGSNDLLDPATG
                     YRLALRLSPEASLQSGTFGYARTQLDASFYQPFTPTVTLAGRIRLGTIIGAGRDRIAP
                     SRRFYAGGGGSVRGYGYQRVGPRDPIFNDPLGGRSLAEFAIEARVRF"
BASE COUNT          238 a          640 c          568 g          220 t
ORIGIN      
        1 tccgacccgc tcgaccagcc gccgctcgat ccgtcggcgc cgctcgatcc cctgcccgat
       61 ctcggcgtcg actggccgga catggagcag ggaaccgacg agggcatcgc cgatgcgccg
      121 gacgccggca tcgccgatgc cgccgccgag cgccgctaca cgatccgcat cgaaggcctg
      181 gacgccgatc tcgaggcggc cgtgaaggcc cagttcgatc cgctctcgac gctcgagcag
      241 aaccggcgcg agccggccaa tgccgcccag atcgaccgcc gcgcccgcga ggatgcggac
      301 ctgctcgccg agctgctccg agcccgcggc ttctacgatg cgctggtgag gacccgggtc
      361 gaggcggcgc cggccggcgg cgaggtcgcg gtgacgctcg agacggagcc gggtcctctc
      421 taccgcttcg ccgaggtcga gctgcccgga ctcgacggcg ccggcggcga ttccgaggcg
      481 ctccgcgacg ccttcggcat cgccgcgggc cagccggtcg atgccggccg cgtgacggcc
      541 ggcgaagcgg cgctgcgtgt cgagctgggc aggcgcggct acgccttcgc ggaggtcggc
      601 gcgatggaca tcgcgctcga tcacgagact cgcaccgcca ccctcaccct gcccgtgaac
      661 ccgaacgggg cgcgccaatt cggtcgaatc ctcgtcgaag gccggccgct gttcagcgcg
      721 aagcacatcc agacgatcgc gcgctggaag ccgggagccg cctatgcagc gccgctcgtc
      781 gaggacctca ggcgggcgct gatcgccacc ggcctcgtct cggccgtggc gatccgcccg
      841 gtcgccggac aggggcccgg gacagtcgac ctcgccgtct ccctcgagcg cgcgccgatg
      901 cgcacgatcg cgggccaggc cggctacggc accggcgagg gcataagggt cgaggcgagc
      961 tggcagcatc gcaacctgct cgcgcccgag ggcgcggtga ccttccgagg agtcctcggc
     1021 acccaggagc agctgatcgg cgcggtcctg cgccgaagca atttcctgca ccgggaccag
     1081 gtgctgaacg cccaggtcgc gatcagccat atcgaccgcg ccgcctatga tgctcggacc
     1141 agcctcctcg ccggatcgat cgagcgccag accaacctca tctggcagaa gaaatggacc
     1201 tggtcggccg gggccgagct gatcgcgacc gacgagcgcg acgtcgacct cgccagcggc
     1261 gagacccgcc ggcgcacctt cttcatcggc gccctgcccg ccagcctctc ctatgacggc
     1321 tcgaacgatc tgctcgaccc ggcgaccggc tatcgcctcg ccttgcggct gtcgccggaa
     1381 gcctcgctcc agtcggggac cttcggctac gcccgcaccc agctcgacgc gagcttctac
     1441 cagcccttca cccccaccgt gaccctcgcc gggcggatcc ggctggggac catcatcggc
     1501 gccggccgcg accgcatcgc gccgtcgcgg cgcttctacg ccggcggcgg cggttcggtg
     1561 cgcggctacg gctatcagcg ggtcggcccg cgcgacccca ttttcaacga cccgctcggg
     1621 ggccgcagcc tggcagagtt cgcgatcgaa gcgcgcgtcc gcttcg
//
LOCUS       DASVQZ010000130         6130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_224089, whole genome shotgun sequence.
ACCESSION   DASVQZ010000130 DASVQZ010000000
VERSION     DASVQZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224089"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VF547_03690"
     CDS             <1..138
                     /locus_tag="VF547_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8261959.1"
                     /translation="SEHRKAELPRWLHQYNWHRPHGSLQTQTPISRLGLDENNLLRLH
                     T"
     gene            complement(135..653)
                     /locus_tag="VF547_03695"
     CDS             complement(135..653)
                     /locus_tag="VF547_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8261960.1"
                     /translation="MSLKQFADTLPDYAKDLRLNLSSLLNDQALGDQRKYGLLLACAH
                     GTGHAPIVAAAEAEAEGKLSREAAEAARAAAAVMAMNNVYYRFVHLASNPEYGTLPAR
                     LRMNVIGAPGIDKADFELFSLAVSAMNGCGMCIDSHERVLQQHKVKSETIQTAVRIAA
                     VMKAAATVHSTL"
     gene            complement(880..1419)
                     /locus_tag="VF547_03700"
     CDS             complement(880..1419)
                     /locus_tag="VF547_03700"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8261961.1"
                     /translation="MLTVGDKFPALTVPVQQGADLPAGETLDLAETNGKWKIVFFWPK
                     DFTFVCPTEIIGYGELAQDFADRDAVLIGASTDTDFVHHAWRKADERLAACDFPWIAD
                     NKKELANALGIVDRDAGVAFRATFVVDPDNVIQHVTVNGLNVGRNPQETLRVLDALQT
                     DELCPCNWQEGEEVLRPAA"
     gene            1553..2458
                     /locus_tag="VF547_03705"
     CDS             1553..2458
                     /locus_tag="VF547_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX8261962.1"
                     /translation="MSTHIPTLKQLQYLVALEDTGHFGRAAEACFVTQSTLSGGIREL
                     ESLIGVVLVERTKRVVRFTPLGARVAAKARRILREADELADMVRAAGKPLAGELRMGV
                     IPTIAPFLLPSLLPRLRREFPELRLYLREETSQAACDGLHRGQLDCVLLALPYACGEV
                     EIEPLLDDRLFVAFPPGEAPAGKAAVPAAAIDEDRLLLLEDGHCLKEHALAACNRPEL
                     RAEAAMLGTSLHTLVQMVANGLGQTLVPEMALRAGILEGTGVAARLLNADHAFRRIAL
                     IWRRGSPREKEFRLLAESLKPPARP"
     gene            complement(2604..3170)
                     /gene="pgsA"
                     /locus_tag="VF547_03710"
     CDS             complement(2604..3170)
                     /gene="pgsA"
                     /locus_tag="VF547_03710"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX8261963.1"
                     /translation="MLTLPNILTLSRIFAVPLLVFLLWKPGWYDYAVTFALYCLVGIT
                     DYFDGYLARAQGTVSRLGIFLDPIADKIMVVGVLVMLVSSRQPHAEPVIHGFHIVPAL
                     IILLREIAVSGLREFLAGLQVSLPVSALAKWKTTFQLVALGALILGGAVPQWAFVHWT
                     GLASLWAAATLTLVTGWDYLRVGLKHMD"
     gene            3324..4595
                     /locus_tag="VF547_03715"
     CDS             3324..4595
                     /locus_tag="VF547_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261964.1"
                     /translation="MQDSMHLLRKRRFLPLFVTQFLGAFNDNLFKTAMVILVTYYVYS
                     DPTKEAVFNAISGAVFILPFFLFSALAGQLADSSDKARIIRIVKTAEIVIMVFGAIGL
                     MIHSIPLMLLALFAMGVHSSFFGPIKYAILPQHLRKDEVLGGTGLVEAGTYVAILMGT
                     IAGGLVKPPVAAAGVLVVAVLGWFAGRQVPPAPPEPDAPVAQMDWHILRASIRLVSAT
                     LHIPRLFLAIVSISFFWMMGAILAAQFPPLVKNVFNANEQVATLFLAIFSVGVGLGSV
                     LINRMLKGRVAAVYSPAAAIAMGLFICHLYYNARGWPIAGGELIDVRTFILIPQAEWV
                     MFDLFGVAVSGGMFVVPLYAFLTTTVPKSETARTVAANNIVNSGAMVLAALVLSAMVQ
                     LGVSVEETLLLVAVSSLGAAWLAWKLHKACD"
     gene            4941..>6130
                     /locus_tag="VF547_03720"
     CDS             4941..>6130
                     /locus_tag="VF547_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8261965.1"
                     /translation="MSGGGQALDVEAALAASVRDPRLMQAGLALAEGQLAVAERLLRS
                     HLKEKPTDIAAIRMMAELAGRLGRYGDAEHLLRRALELAPGFTAARANLATVLYRQNR
                     PAEAIEMLDTLLDGEPANPAHQNLKAAALGRIGGYEEAISLYAAVLERFPDQPKVWMS
                     YGHVLKTVGRRDDSIAAYRRAIEAAPTLGEAWWSLANLKTVRLGEADLAAMEAALTRA
                     DLVDEDRFHLHFALGKALEDEGEARRAFAHYAEGNRLRRAAIAYDPEEIERHVERSVA
                     LFTAAFFAERAGRGCACPEPIFILGMPRAGSTLVEQILASHSAVEGTMELPDIPALAK
                     RLGGRKLKSDSNAYPESLATLSTADLAALGEEYLERTRIQRKTGRPLFIDKMPNNWRD
                     SGFI"
BASE COUNT          954 a         2069 c         2100 g         1007 t
ORIGIN      
        1 tcggaacacc gcaaggccga gctgcccaga tggctccacc aatataactg gcaccggcca
       61 cacggcagcc tacaaaccca aacacccatc agccgactcg gcctggacga gaacaacctg
      121 ttgaggctcc acacctagag cgttgagtgc accgtcgcgg ccgccttcat cacggcggcg
      181 atgcgcacgg cggtctggat cgtctcggac ttgaccttgt gctgctggag gacgcgctcg
      241 tggctgtcga tgcacatgcc gcagccgttc atcgcgctga ccgcgaggct gaacagctcg
      301 aaatccgcct tgtcgatgcc gggggcgccg atcacgttca tcctgaggcg ggccgggagc
      361 gtgccgtatt ccgggttcga ggcgaggtgg acgaagcggt agtagacgtt gttcatcgcc
      421 atcaccgccg cggcggcgcg ggcggcctcg gccgcctcgc ggctcagctt gccctccgct
      481 tccgcctcgg cggcggccac gatcggggcg tggccggtgc cgtgggcgca ggcgagcaac
      541 aggccgtatt tgcgctggtc gccgagcgcc tggtcgttga ggagcgagga gaggttgagc
      601 cgaaggtcct tggcgtaatc gggaagcgtg tcggcaaatt gcttgaggga catttggtgc
      661 ctttctttgc tcctcccctg aacggggagg gggaccgcga cgcgaagcgg cgtgtggagg
      721 ggggcccgga gttccaagcg gcggacaatc ggggcccctc caccatgctt cgcatggtac
      781 ccctccccgt tccggggagg attgaactaa aggggcgcgg gaaccgaagc tcccgcgccc
      841 cccgtcgcga ggtctgctgc cttggcggcc caggggggct caagccgccg ggcgcaggac
      901 ctcctcgcct tcctgccagt tgcaggggca gagctcgtcg gtttggagcg cgtcgagcac
      961 gcgcagcgtc tcctgcgggt tacggccgac gttgaggccg ttcacggtga cgtgctggat
     1021 gacgttgtcg gggtcgacga cgaaggtggc gcggaaggcc actccggcgt cgcggtcgac
     1081 tatcccgagc gcgttggcca gctccttctt gttgtccgcg atccagggga agtcgcaggc
     1141 ggcgagccgc tcgtcggcct tgcgccaggc atggtggacg aagtcggtgt cggtcgaggc
     1201 gccgatcagg accgcgtcgc ggtcggcgaa atcctgggcg agctcgccat agccgatgat
     1261 ctcggtcggg cagacgaagg tgaagtcctt cggccagaag aagacgatct tccatttgcc
     1321 gttggtctcg gcgagatcga gcgtctcgcc cgcgggcaga tcggcgccct gctggacggg
     1381 aacggtgagg gcggggaact tgtcgccgac ggtaagcatg aaaacgagcc tcctgctggg
     1441 gtctgttatg ataggatttt ttgcggtgca gcatgactgc cgcaccgccc atatgggggc
     1501 tggcgctcat cggacaaatt gattagttcg cgatctttga tcgggtgagc cgatgagtac
     1561 ccacatcccc acgttgaagc agcttcaata tctggtcgcc ctcgaggaca ccggccattt
     1621 cggccgggcg gccgaggcct gcttcgtcac ccagtcgacc ctgtccggcg ggatccggga
     1681 gctcgagagc ctgatcgggg tggtgctggt ggagcggacc aagcgggtgg ttcgcttcac
     1741 tccgctcggt gcccgggtcg ccgccaaggc gcgccggatc ctccgcgagg ccgacgagct
     1801 cgccgacatg gtgcgcgcgg ccggcaagcc gctggccggc gagttgcgga tgggggtgat
     1861 cccgaccatt gcccccttcc tcctgcccag cctgctgccc aggctccgcc gcgaattccc
     1921 ggagcttcgg ctctatctgc gcgaggagac cagccaggcg gcctgcgacg gcctccaccg
     1981 cggccagctc gactgcgtcc tgctcgcctt gccttatgcc tgcggcgagg tcgagatcga
     2041 gccgctgctc gacgaccggc tgttcgtcgc cttcccgccg ggcgaggcgc cggccggcaa
     2101 ggccgcggta ccggccgcgg cgatcgacga ggaccggctg ctcctgctcg aggacggcca
     2161 ttgcctcaag gagcatgcgc tcgccgcctg caaccggccc gagctgaggg cggaggcggc
     2221 gatgctcggc acctcgctcc acaccctcgt ccagatggtc gccaacggcc tcggccagac
     2281 tttggtgccg gaaatggcgc tgagggccgg gatcctcgag ggcaccggag tcgccgcacg
     2341 gctgctcaac gccgaccacg ccttccggcg catcgccctc atctggcgcc ggggcagccc
     2401 gcgcgagaag gagttccggc tgctcgccga gtcgctgaag ccgccggccc gaccttagaa
     2461 gccgcctttc cctcgtcggc cttggtgcca gacccttgat tgcgaatgcc attatggccc
     2521 tctcggcggc ctggcaggga tcggctcgcc gatgtctgag gaccggttcg cctaaacgct
     2581 tcgcagagcc ggcggcgccg ggctcagtcc atgtgcttga ggcccacgcg cagatagtcc
     2641 cagcccgtca ccaaggtgag cgtcgcggcc gcccacaggc tcgcgaggcc ggtccagtgg
     2701 acgaacgccc attgcggcac cgccccgccg aggatgagcg cacccagggc gaccagctgg
     2761 aaggtcgtct tccatttcgc cagggcgctg accggcagcg acacctgaag accggcgagg
     2821 aactcgcgaa ggccggagac ggcgatctcg cgaagcagga tgatcagcgc cggcacgatg
     2881 tggaagccgt ggatcaccgg ctcggcgtgc ggctggcggc tcgagacgag catgacgagg
     2941 actccgacga ccatgatctt gtcggcgatc ggatcgagga agatgccgag cctcgacacc
     3001 gtcccctggg cgcgggcgag gtagccgtcg aaatagtcgg tgatgccgac caggcagtag
     3061 agggcgaagg tgaccgcata atcgtaccag ccgggcttcc acagcaggaa cacgagcagg
     3121 ggcaccgcga agatccggga cagggtgagg atgttgggaa gggtcagcat cggctcggcc
     3181 tgcgtcctta gcgggtcggg gggcggctgt gaacgtgcac cgtcatcccg gcggaagtgc
     3241 ggaacgtttg accggggcgg cgaagcggct atgaccgccc gcgcatgtcg tcacgcccct
     3301 ccgccggccg cgcctgagcg caagtgcagg attcgatgca cctgcttcgc aagcggcgct
     3361 tcctgccgct gttcgtcacc cagttcctgg gggcgttcaa cgacaacctg ttcaagacgg
     3421 cgatggtgat cctggtcacc tattacgtct attccgaccc gacgaaggag gcggtgttca
     3481 acgccatatc gggcgccgtc ttcatccttc ccttcttcct cttctcggcg ctggccgggc
     3541 agctcgccga ttccagcgac aaggcgcgga tcatccggat cgtcaagacg gccgagatcg
     3601 tgatcatggt cttcggcgcg atcggcctga tgatccattc gatcccgctc atgcttctcg
     3661 ccctgttcgc gatgggcgtc cattcctcgt tcttcggtcc gatcaaatat gcgatcctgc
     3721 cgcagcatct gaggaaggac gaggtgctcg gaggcaccgg gctggtcgag gcggggacct
     3781 atgtcgcgat cctgatgggg acgatcgccg gcgggctggt caagccgccg gtggccgccg
     3841 ccggagtgct ggtggtggcg gtgctcggct ggttcgccgg gcggcaggtg ccgccggcgc
     3901 cgcccgagcc cgacgctccg gtcgcccaga tggactggca catcctgcga gcctcgatcc
     3961 ggctggtcag cgccaccttg cacataccgc gcctgttcct cgccatcgtc tcgatcagct
     4021 tcttctggat gatgggcgcg atcctcgccg cccagttccc gccgctggtc aagaacgtgt
     4081 tcaacgccaa cgagcaggtg gcgacgctct tcctcgccat cttctcggtc ggagtgggcc
     4141 tcggctcggt gctgatcaac cggatgctga agggccgggt ggcggcggtc tattcgcccg
     4201 ccgcggcgat cgcgatgggc ctgttcatct gccatctcta ctacaatgcc cgcggctggc
     4261 cgatcgccgg cggcgagctg atcgacgtgc gcaccttcat cctcatcccg caggccgaat
     4321 gggtgatgtt cgacctgttc ggcgtcgccg tctcgggcgg catgttcgtg gtgccgctct
     4381 acgccttcct gacgacgacg gtgcccaagt ccgaaacggc gcggacggtg gcggcgaaca
     4441 acatcgtcaa ttcgggggcg atggtgctgg ccgccctggt gctgtcggcg atggtgcagc
     4501 tgggcgtctc ggtcgaggag acgctgctcc tcgtcgcggt ctcgagcctc ggcgccgcct
     4561 ggctcgcctg gaagctgcac aaggcctgcg actgaaggat gcttcccgtc acggctgacg
     4621 agaggtcggg tggagccggg tgaaaggcca gccggcctaa gctggtgtcg ttacgcccgc
     4681 ggcggccggg aaggagaaat caggcaataa aggccgaaaa gaagacgacc ccgcctgcga
     4741 aggtcagcag gaagaggcga aggttctcgg agtcgggaat caggcggtcc atcgccgtcg
     4801 gttaacgtgc tctttaccac gccggcaagg ggccgccggg acctccgagg gccctgtccc
     4861 gccgcggcac agcacgcctg ccgatccttc cgccccgccg ggactcgcgc ccgcccgccg
     4921 ccctcctcta tagccgggcc atgagcggtg gaggacaagc cctggacgtc gaggcggcgc
     4981 tggccgcctc ggtccgcgat ccgcggctga tgcaggccgg ccttgcgctg gccgaggggc
     5041 agctggccgt cgccgagcgc ctgctccggt cccacctcaa ggaaaagccc accgacatcg
     5101 ccgcgatccg gatgatggcc gagctggccg gccggctcgg ccgctacggc gacgccgagc
     5161 atctgcttcg ccgggccctc gagctcgccc ccggcttcac cgccgcccgc gccaatctcg
     5221 cgacggtgct ctaccgccag aaccggcccg ccgaagcgat cgagatgctc gacaccctgc
     5281 tcgacggcga gccggccaac cccgcgcacc agaacctcaa ggcggccgcg ctgggacgca
     5341 tcggcggcta tgaggaggcg atctcgctct atgccgccgt cctcgagcgc tttcccgacc
     5401 agcccaaggt gtggatgagc tacggccacg tgctcaagac ggtcggccgc cgcgacgaca
     5461 gtatcgccgc ctatcgacgc gccatcgagg ctgcgcccac gctcggcgag gcgtggtgga
     5521 gcctcgccaa cctcaagacg gtccggctgg gcgaggccga cctggcggcg atggaggcgg
     5581 ccctgacgag ggcggacctc gtcgacgagg atcgattcca ccttcatttc gcgctcggca
     5641 aggcgctcga ggacgaaggc gaggcgcggc gcgccttcgc ccattacgcg gagggcaacc
     5701 gcttgcgccg ggcggcgatc gcctacgatc ccgaggagat cgagcgccat gtcgagcgca
     5761 gcgtcgcctt gttcaccgcc gccttcttcg ccgagcgcgc cggccgcggc tgcgcctgcc
     5821 ccgaaccgat cttcatcctg ggaatgccgc gggccggctc gacgctcgtc gagcagatcc
     5881 tcgccagcca ttcggcggtc gagggaacga tggagctgcc ggacatcccg gcgctcgcca
     5941 agcgcctcgg cgggcgcaag ctcaagtccg attccaatgc ctatccggaa agcctcgcca
     6001 ccctctccac cgccgatctc gccgcgctgg gtgaggaata tctcgagcgc acccggatcc
     6061 agcgcaagac cggccgaccc ctgttcatcg acaagatgcc gaacaactgg cgggactcgg
     6121 gcttcatcca
//
LOCUS       DASVQZ010000131         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_413953, whole genome shotgun sequence.
ACCESSION   DASVQZ010000131 DASVQZ010000000
VERSION     DASVQZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413953"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1380
                     /locus_tag="VF547_03725"
     CDS             106..1380
                     /locus_tag="VF547_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261966.1"
                     /translation="MASAAPHPLRIRDFRFYWTARFASTVAQVAMIVVIGWQVYDIAR
                     RTMDVEQAALQLGFIGLVQFVPLFALTLVTGWVADRVDRRVIARASIALELLCASALA
                     WLTWSGQVTLLWLFVVAALLGVARAFAGPALGALAPNLVPRDSLPRAIALSSIAWQTG
                     AIAGPAVGGYLYAWDPHMPYGVAAALFALALLCMLMIRPIARSAIAGSANPWRQMVDG
                     LHYVRRNRLVLGAISLDLFAVLLGGATAMLPVFARDVLHSDATALGHLRAAPAVGATL
                     TALWFSWRPLKDDVGAKMLAAVAVFGAATVAFGLSRDMILSIACLALLGSADMVSVYV
                     RQSLIQIHTPDAMRGRVGAASTLFISASNELGEAESGFLAALIGPVVAVVAGGVGAIA
                     VTLLWARWFPELRRARTFDPPDPVLRDGAAPL"
     gene            complement(1383..>1576)
                     /locus_tag="VF547_03730"
     CDS             complement(1383..>1576)
                     /locus_tag="VF547_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PKHD-type hydroxylase"
                     /protein_id="HEX8261967.1"
                     /translation="VELSPLGDHDGGDLEIVPDTIGRPRNLPRGGARFFPSRALHRVT
                     PVRRGLRHALVIWAGAPAG"
BASE COUNT          163 a          614 c          542 g          257 t
ORIGIN      
        1 gcgcccgact cgatgaactt catgaacttt gccgtcgagg cactcgcggc gaggccgccg
       61 atcggcggtt gcggcccccc gcgccgcgcc ctaagcgccg cgcccatggc ctccgccgcc
      121 ccgcatcccc tgcgcatccg cgacttccgc ttctactgga cggcgcgctt cgcctcgacg
      181 gtggcgcagg tggcgatgat cgtcgtcatc ggctggcagg tctacgacat cgcccggcgg
      241 acgatggacg tcgagcaggc ggcgctgcag ctcggcttca tcggcctcgt ccagttcgtg
      301 cccctgttcg ccctcaccct ggtgaccggc tgggtggccg accgggtcga ccgccgggtc
      361 atcgcccgag cctcgatcgc cctggagctg ctctgcgcct cggccctcgc ctggctcacc
      421 tggagcggcc aggtcacgct gctctggctg ttcgtcgtcg ccgccctgct cggagtggcg
      481 cgggccttcg ccgggccggc gctcggcgcg ctcgcgccga acctcgtgcc gcgggactcg
      541 ctgcccaggg cgatcgcgct gagctcgatc gcctggcaga ccggcgccat cgccgggccg
      601 gccgtggggg gctatctcta cgcctgggat ccgcacatgc cctatggcgt ggcggcggcg
      661 ctgttcgcct tggcgctgct gtgcatgctg atgatccggc cgatcgcccg aagcgccatc
      721 gcgggctcgg ccaatccctg gcggcagatg gtcgacggcc tccactatgt gcgccgcaac
      781 cgcctcgtcc tcggcgccat ctcgctcgat ctgttcgcgg tcctgctcgg cggagccacg
      841 gcgatgcttc cggtcttcgc ccgcgacgtc ctccattccg acgcgaccgc gctcggccat
      901 ctgcgcgccg cgccggcggt cggggcgacg ctgaccgcgc tgtggttctc ctggcggccg
      961 ctcaaggacg atgtgggcgc caagatgctc gccgcggtcg ccgtgttcgg ggccgccacc
     1021 gtcgccttcg gactgtcgcg cgacatgatc ctgtcgatcg cctgcctcgc cctgctcggc
     1081 tcggccgaca tggtctcggt ctacgtccgc cagtcgctga tccagatcca cacgcccgat
     1141 gcgatgcgcg gccgggtcgg cgccgcctcg accctgttca tctccgcctc gaacgagctc
     1201 ggcgaggccg aaagcggctt cctcgcggcg ctgatcggcc cggtcgtcgc cgtggtcgcc
     1261 ggcggcgtcg gagcgatcgc cgtcaccctc ctgtgggcgc gctggttccc cgagctgcgc
     1321 cgagcccgca ccttcgatcc gcccgatccc gtgctccgcg acggcgcggc gccgctctag
     1381 cgtcagccgg cgggggctcc cgcccagatc accagggcat ggcgcaggcc ccgccgcacc
     1441 ggagtcaccc ggtgcagcgc ccgcgacggg aagaagcggg cgccgccgcg gggcaggttc
     1501 cgcggccggc cgatcgtgtc ggggacgatc tcgaggtcgc cgccgtcatg gtcgccgagc
     1561 ggcgacagct cgaccg
//
LOCUS       DASVQZ010000132         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_127606, whole genome shotgun sequence.
ACCESSION   DASVQZ010000132 DASVQZ010000000
VERSION     DASVQZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127606"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="VF547_03735"
     CDS             <1..1725
                     /locus_tag="VF547_03735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8261968.1"
                     /translation="VADLYRGFLDEAAAERADAVPLLARLVPVYQARSRDDVARLMGR
                     ALGGFGSSFFGPGVSDDAKKPDVYALYLRQAGLGLGDRDFYVDPKFKPQRDRYLQYVE
                     QMLGMAGWPDPAGAARSVFALETKIAEAHWTRAESRNRDKTYNLMTRAELEAYAPGFP
                     WATFLDAAGVGRAERAIVAQNTAFPRIARLFAGTDLQTLKAWQAFHVADKMAPLLSKR
                     FVDAQFEFRSKFLNGQPQQRERWKRGVTFVENAMGEAIGRDYVRLYFPPASKAKMDAL
                     VANLRTAMGSRIRALQWMGPATKAEALKKLEGFNVKIGYPEKWRDYSELAVVPGDLVG
                     NAERAARFEWDYRRNRIDQPVDKAEWGMTPQTVNAYYNSVKNEIVFPAAILQPPFFDP
                     DADPAVNYGGIGGVIGHEIVHGFDDQGRKSDYAGVLRDWWTPEDAAKFEAQAAKLGAQ
                     YEAYTFPNLPGVRINGRASMGENIGDLGGITLGLEAYRTSLAGKPAPTVDGFTGDQRV
                     FLGWAQVWRSLFRDDALRQQLVGDPHSPGMIRAFAPLRNVDAWYEAFGVKPGDKLYIP
                     PEERVRIW"
     gene            complement(1689..2402)
                     /locus_tag="VF547_03740"
     CDS             complement(1689..2402)
                     /locus_tag="VF547_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261969.1"
                     /translation="MIPRLLIDSAPIPGGGGELRLVQRGADFWIMLGANALMSTRLRG
                     SEEALATLVCERLAGRPRPALLIGGLGMGFTLRAALGALGAGSRLVVAELVPAIVRWA
                     RGPMAQVHGASLDDPRVEIVEGDVRDLIDAARAEYDAILLDVDNGPEALSRETNDRLY
                     DLAGLRRARSALRPGGILAVWSSGPDKAFARRLAEAGFAVEEVTVRAHAGRSGAKHVI
                     WLAAARGGHQIRTRSSGGM"
     gene            complement(2399..4267)
                     /locus_tag="VF547_03745"
     CDS             complement(2399..4267)
                     /locus_tag="VF547_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX8261970.1"
                     /translation="MLSLNNITVRLGGRTILDGATAALPPRSRVGLVGRNGAGKSTLV
                     RVVAGLLGTDEGAAEMPRGARIGYIAQEAPAGTATPFETVLAADLERAALLAEEAGCT
                     DLHHIGEIHERLNAIDAYAAPSRAARILVGLGFDEEAQHRPLDSFSGGWRMRVALAAL
                     LFSAPDLLLLDEPSNHLDLEAVLWLEDFLKSYRATILVVSHERDFLNNVVDHILHLDR
                     GKLTLYPGGYDAFERQRAERQAQLASAREKQMAERAKLQDYIARNSARASTARQAQSR
                     VKALARMQPIAELLDDPSLSFGFPDPQPLRPPLVTLDMASVGYGGTPVLSRLNLRLDP
                     DDRIALLGRNGNGKTTLARLLAAQLAPMEGEVVRSQKLRIGYFTQYQVEELDGSATPF
                     QHMARLMKGATPAAVRSQLGRFGFSGDKALTEVRRLSGGERARLALALITREAPHLLI
                     LDEPTNHLDVDAREALVQALNGYSGAVIVVSHDRHMLELIADRLVLVEGGTAREFDGS
                     IEDYTALVLARDAAGPAGAPKPNRKEERRAAAVAREKGQALRARARAAEAEVARLTGL
                     RSAIDLAMFDPARAEKPFADLTMTELMKRRAQVERELEAAETQWLEASEALESLAA"
     gene            complement(4290..4364)
                     /locus_tag="VF547_03750"
     tRNA            complement(4290..4364)
                     /locus_tag="VF547_03750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4330..4332),aa:Val,seq:gac)
     gene            4574..>5218
                     /locus_tag="VF547_03755"
     CDS             4574..>5218
                     /locus_tag="VF547_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8261971.1"
                     /translation="MRAQRARALERPAHAPVVSRMRAPAADRQGDGAGGATSHGFDLT
                     AVPAVAGLPISSPQEPAEKEADRTADRVMRGEDVRPAPMPAAAARLERKCDACEEEEG
                     RAGAEIRREPLGAAPPAERAPPAVAAALRSPGRPLDAEVRADFEPRFGTSFADVRVHH
                     DSSAAIAARSIGARAFTLGREIAFGAGEYAPETRGGRFLLAHELAHVVQNGGNAP"
BASE COUNT          767 a         1874 c         1855 g          722 t
ORIGIN      
        1 gtcgccgatc tctaccgcgg attcctagac gaggccgcgg ccgaacgcgc cgacgccgta
       61 ccgctgctcg cccggctggt gccggtctat caggcaagga gcagggacga cgtcgcccgg
      121 ctgatgggcc gggcgctggg cggcttcggg tcgagcttct tcggcccggg cgtcagcgac
      181 gacgccaaga agcccgacgt ctacgccctc tatctgcgcc aggccggcct cggcctcggc
      241 gaccgcgatt tctacgtcga cccgaaattc aagccgcagc gcgaccgcta tctccaatat
      301 gtcgagcaga tgctgggcat ggccggctgg cccgacccgg ccggcgccgc gcgcagcgtc
      361 ttcgcgctcg agacgaagat cgccgaggcg cactggacga gggccgagag ccgcaaccgc
      421 gacaagacct acaatctgat gacccgggcc gagctcgagg cctatgcgcc gggcttcccc
      481 tgggcgacct tcctcgacgc cgccggggtg ggccgcgccg agcgcgccat cgtcgcccag
      541 aacaccgctt tcccaaggat cgcacggctg ttcgccggga ccgacctcca gaccctgaag
      601 gcgtggcagg ccttccacgt cgccgacaag atggcgcccc tcctgtcgaa gcgcttcgtc
      661 gacgcccagt tcgagttccg ctcgaaattc ctcaacggcc agccgcagca gcgggagcgg
      721 tggaagaggg gcgtcacctt cgtcgagaac gcgatgggcg aggcgatcgg gcgcgactac
      781 gtccgcctct atttcccgcc cgcttccaag gcgaagatgg acgccctcgt ggccaacctg
      841 cgcaccgcca tgggctcgcg gatccgcgcc ctgcagtgga tggggcccgc caccaaggcc
      901 gaggcgctca agaagctcga gggtttcaat gtcaagatcg gctatcccga gaaatggcgc
      961 gattattcgg agcttgcggt ggtcccgggc gacctcgtcg gcaatgccga gcgcgccgcc
     1021 cgcttcgaat gggattatcg ccgcaaccgg atcgaccagc cggtcgacaa ggccgagtgg
     1081 ggcatgaccc cgcagaccgt gaacgcctat tacaacagcg tgaagaacga gatcgtcttc
     1141 ccggccgcca tcctccagcc ccccttcttc gacccggacg cggatccggc ggtcaattac
     1201 ggcggcatcg gcggagtcat cggccacgag atcgtccacg gcttcgacga ccagggccgc
     1261 aagtccgatt atgccggcgt gcttcgcgac tggtggacgc ccgaggacgc cgccaagttc
     1321 gaggcgcagg cggcgaagct cggggcccag tacgaggcct acaccttccc caatctgccc
     1381 ggagtccgga tcaatgggcg agcctcgatg ggcgagaata tcggcgacct cggcggcatc
     1441 accctcggcc tcgaggccta tcgcacctcg ctggccggca agcccgcacc gacggtcgac
     1501 ggcttcaccg gagaccagcg cgtcttcctc ggctgggcgc aggtctggcg cagcctgttc
     1561 cgcgacgacg cccttcgcca gcagctggtc ggcgacccgc actcgccggg catgatccga
     1621 gcgttcgccc cgcttcgcaa cgtcgacgcc tggtacgagg cgttcggcgt gaagccgggc
     1681 gacaagctct acattccgcc ggaggagcgg gtgcggatct ggtgaccgcc gcgggcggcg
     1741 gcgagccaga tcacatgctt ggcgccgctg cgcccggcat gggcgcggac ggtgacctcc
     1801 tcgacggcga agcccgcctc ggccagccga cgggcgaagg ccttgtcggg gcccgaggac
     1861 cagacggcca ggattccgcc cggccgcagg gccgagcgcg cgcggcgcag cccggccagg
     1921 tcgtagaggc ggtcgttggt ctcgcggctg agcgcctccg gtccgttgtc gacgtcgagc
     1981 aggatcgcgt catattcggc gcgggcggcg tcgatcaggt cgcgcacgtc gccttcgacg
     2041 atctcgaccc gaggatcgtc caggctcgcg ccgtggacct gggccatcgg tcctcgcgcc
     2101 cagcgaacga tcgccggcac cagctccgcc acgaccagcc tcgagccggc gcccagcgcg
     2161 ccgagcgcgg cgcgaagggt gaagcccatt ccgagcccgc cgatcagcag cgccggccgg
     2221 ggccgtccgg caaggcgctc gcagaccagg gtggcgagcg cctcctccga gccccgcagc
     2281 cgggtgctca tcagcgcatt ggccccgagc atgatccaga agtcggcgcc gcgctggacg
     2341 agccgcagct cgccgccgcc gccgggaatc ggcgcgctgt cgatcagcag gcgggggatc
     2401 atgcggcgag gctctccagc gcctcgctcg cctccagcca ttgcgtctcg gccgcttcca
     2461 gctcgcgctc gacctgggcg cgccgcttca tcagctcggt catggtgagg tccgcgaacg
     2521 gcttctccgc ccgggccggg tcgaacatcg ccaggtcgat ggcgctgcgc agcccggtca
     2581 gccgggcgac ttccgcctcc gccgcccggg ctcgggcgcg caacgcctgc cccttctccc
     2641 gtgcgacggc ggcggcgcgc cgttcctcct tgcggttcgg cttgggcgcg cccgccggcc
     2701 cggcggcatc tcgggccagc accagggccg tataatcctc gatgctgccg tcgaattcgc
     2761 gcgccgtgcc gccctcgacc agcaccagcc ggtcggcgat cagctccagc atgtggcggt
     2821 cgtggctgac cacgatgacc gctccggaat agccgttcag cgcctgcacc agcgcctcgc
     2881 gggcgtcgac gtcgagatgg ttggtcggct cgtcgaggat gaggaggtgc ggcgcctccc
     2941 gggtgatcag ggccagcgcc agtcgcgccc gctcgccgcc ggagaggcgg cgcacctcgg
     3001 tcagcgcctt gtcgccggag aagccgaagc ggccgagctg cgagcgcacc gccgccggag
     3061 tcgccccctt catcagccgg gccatgtgct ggaacggcgt ggcggagccg tccagctcct
     3121 ccacctgata ttgggtgaaa tagccgatcc gaagcttctg gctgcgcacc acctcccctt
     3181 ccatcggcgc gagctgcgcc gccagcaggc gcgcgagcgt ggtcttgccg ttgccgttgc
     3241 ggccgagcag ggcgatccgg tcgtccgggt cgaggcgcag gttgagccgg ctgagcaccg
     3301 gcgtgccgcc atagccgacc gatgccatgt cgagcgtgac cagcggcggg cgcaggggct
     3361 gcgggtcggg aaagccgaag gacaaggacg gatcgtcgag cagctcggcg atcggctgca
     3421 tccgggcgag cgccttgacc cgcgactgcg cctgacgggc ggtcgaggcg cgcgccgagt
     3481 tgcgggcgat gtaatcctgg agcttggcgc gctccgccat ctgcttctcg cgcgccgagg
     3541 ccagctgcgc ctgccgttcc gcccgctgcc gctcgaacgc gtcgtagccg ccggggtaga
     3601 gggtgagctt gccgcgatcg agatggagga tgtgatcgac gacgttgttg aggaagtcgc
     3661 gctcgtggct gacgacgagg atggtcgccc ggtagctctt caggaaatcc tcgagccaca
     3721 gcacggcttc cagatcgagg tggttcgacg gctcgtcgag cagcagcagg tccggcgccg
     3781 agaacagcag agccgcgagc gcgacccgca tccgccagcc gccagagaag ctgtcgagcg
     3841 gccgatgctg ggcctcctcg tcgaagccga ggccgacgag gatccgggcc gcgcgcgacg
     3901 gcgccgcata ggcgtcgatc gcgttcagcc gctcgtggat ctcgccgata tggtggaggt
     3961 cggtgcagcc cgcctcctcg gcgagcaggg cggcccgctc caggtcggcc gcgagcaccg
     4021 tctcgaacgg agtcgcggtg cccgccggcg cctcctgggc gatgtagccg atgcgcgctc
     4081 cgcgcggcat ctcggccgcg ccctcgtcgg tcccgagcag gcccgcgacc acccgcacca
     4141 gagtcgattt cccggcgccg ttgcggccga ccaggccgac tcggctgcgc ggcggcagag
     4201 cggccgtcgc gccgtcgagg atggtccgcc cgccgaggcg aaccgtgatg ttgttgaggg
     4261 aaagcataga gcgccgcgag aggggaaaat ggtgggcgtg gcaaggattg aacttgcgac
     4321 ccctgcgatg tcaacacagt gctctaccac tgagctacac gcccactcgg ggcgcatgta
     4381 gcggcggccc cggtggggtg caaccccacg gcgccagtga ttgcgcggct cgctccggtc
     4441 cggcgccaaa tccgcttgac gccgcccgcc ggctggcgga ccctgcggta gcctcgggcc
     4501 caagcgttgg agatccagct atgccggccg cagcggatac ccggcccggc gggcagggcc
     4561 aaaggggacg acggtgaggg cgcagcgcgc cagggcgctc gagcgccccg ctcacgcgcc
     4621 ggtcgtcagc cggatgcggg cgccggcggc cgatcggcag ggagacggcg ccggcggggc
     4681 gacgagccac ggcttcgacc tgaccgcggt tccggccgtg gccggactcc cgatcagctc
     4741 tccccaggag ccggccgaga aggaggcgga ccggacggcc gaccgggtga tgcgcgggga
     4801 agatgtccga cccgccccga tgccggcagc ggcggcgcgg ctggagagga aatgcgacgc
     4861 ctgcgaagag gaggaaggcc gggccggcgc ggaaatccgc cgcgagccgc tcggcgcggc
     4921 gcctcccgcc gaacgggctc cgccggcggt ggccgccgcg ctccgcagtc cgggccgccc
     4981 gctcgatgcg gaggttcgcg ccgacttcga gccgcgcttc gggacgagtt tcgccgacgt
     5041 tcgggtccac cacgattctt cggcggccat cgccgcccgc tcgatcgggg cgcgggcttt
     5101 caccctcggc cgcgaaatcg ccttcggggc cggcgaatat gcgcctgaga cccgcggcgg
     5161 ccggttcctg ctggcgcacg agctcgcgca tgtcgttcag aacggcggaa acgcgccc
//
LOCUS       DASVQZ010000133         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_127725, whole genome shotgun sequence.
ACCESSION   DASVQZ010000133 DASVQZ010000000
VERSION     DASVQZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127725"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..884
                     /locus_tag="VF547_03760"
     CDS             9..884
                     /locus_tag="VF547_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261972.1"
                     /translation="MTDLVAAELAEPVDPRVSAMAGAIAALYPEAARAVLFYGSCLRE
                     QQLEGLMLDFYLIVSDYRAAYGKGWLAAANRLIPPNVFPFENEGLVAKYAVLSEADFA
                     RECSLEARSVSVWARFAQPSRLVWTSGPAAQAAALAAVVEAAPTLFALARPLAPEANE
                     PLAIFRSGFALTYGAELRAERGNRSQSIVEADPERYRRLGDAVLAERPGIGLEATAPA
                     RRAWRARQRKGKALTVLRLVKASGTFAGGVDYIAWKINRHAGTKIEVKPWQRRHPLLA
                     GLILLPRLLRRGAVR"
     gene            complement(841..>2566)
                     /locus_tag="VF547_03765"
     CDS             complement(841..>2566)
                     /locus_tag="VF547_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8261973.1"
                     /translation="DWVYAGNQIAQLRSLVAGTPEQLARVEKLKSLYQQRGVGFAAAA
                     AAAAKHKATGGIPLFYQAGQSGLGGEVRDLLTGIATAERQDLRGRMADTRASSDRADE
                     LTDWFGWLAVMIGIGALTLGFLAFRAMRERIEALREADSESSRAGKLEQAVRERTQEL
                     SAANERLLSEAAEREAAEAQLRQVQKMEAVGQLTGGIAHDFNNMLAVVVGGLDLARRR
                     LSGDRREVEMHLDNAMEGATRAAALTRRLLAFARAEPLLPSNLAPSELIEGMLDLVDR
                     TIGERITVRTRAPAEPWPVRVDTNQLENAILNLAVNGRDAMDGEGELRIEVDNVTLAD
                     NQVNALPAGDYVRLSVADTGSGMPPEVLERVFEPFFTTKPVGKGTGLGLSQIFGFARQ
                     SGGDVAIDSEVGRGTIVSLYLPRAEPAENEGQAAAAHAPAGEAEDAPAAGTSVLVVED
                     DPRVSRATVAALEELGYRPIPCSSGREALDALSAGPAVELIITDVMMPEMTGTELVRK
                     ASALYPWIAILFVTGYVGEAGDADDLAGYDILRKPFTVSGLAEAVRVALARRPIEQPP
                     AARAAAGG"
BASE COUNT          325 a          882 c          977 g          382 t
ORIGIN      
        1 cgccccgcct gaccgacctc gtcgccgccg agctggccga gccggtggac ccccgcgtct
       61 ccgcgatggc gggcgcgatc gcggcgctct acccggaggc ggcgcgggca gtgcttttct
      121 acggctcctg cctgcgcgag cagcagctcg aggggctgat gctcgatttc tacctgatcg
      181 tctcggatta ccgcgccgcc tacggcaaag gctggctggc ggcggcgaac cggctgatcc
      241 cgcccaacgt gttcccgttc gagaatgagg ggctggtggc gaaatatgcg gtgctgagcg
      301 aggcggactt cgcacgcgag tgcagcctcg aggcgcgcag tgtgtcggtg tgggcgcggt
      361 tcgcgcagcc ttcgcggctc gtctggacga gcgggcccgc ggcgcaggcg gcggcattgg
      421 ccgccgtggt ggaagccgct ccgacgctgt tcgcgctggc gcgcccgctg gcgccggagg
      481 cgaacgagcc gctcgccatc ttccgctccg gcttcgcgct gacctacggg gccgagctgc
      541 gcgccgagcg ggggaatcgc tcccagtcga tcgtcgaggc ggacccggag cgctaccgtc
      601 gcctcggcga cgccgtgctc gccgagcggc ccggcatcgg gctcgaggcc accgcgccgg
      661 cgcggcgcgc ctggcgcgcg cggcagcgga agggaaaggc gctgacggtg ctgcggctgg
      721 tgaaggcgag cggcaccttc gccggcgggg tcgactacat cgcctggaag atcaaccggc
      781 acgcggggac gaagatcgag gtgaagccgt ggcagcggcg gcatccgctg ctcgccgggc
      841 tcatcctcct gccgcggctc ttgcggcggg gggctgttcg ataggccggc gcgcgagcgc
      901 cacgcgcacc gcttcggcga gcccggagac ggtgaagggc ttgcgcaaga tgtcgtagcc
      961 ggcgaggtcg tccgcgtcgc cggcctcgcc gacatagccg gtgacgaaga ggatcgcgat
     1021 ccagggatag agggcgctcg ccttgcggac gagctcggtg ccagtcatct ccggcatcat
     1081 cacgtcggtg atgatcagct ccaccgccgg gcccgccgag agcgcgtcga gcgcctcgcg
     1141 cccgctggag cagggaatgg gtcggtagcc gagctcctcc agggcggcga cggtcgcgcg
     1201 gctgacgcgg ggatcgtcct cgaccaccag cacgctcgtg ccggcggccg gcgcgtcctc
     1261 ggcctcgccg gcgggggcgt gcgccgcggc ggcctggccc tcgttttcgg ccggctccgc
     1321 gcgggggagg tagagggaga cgatggtgcc tctgcccacc tcggagtcga tcgccacgtc
     1381 gccgccggac tgccgggcga agccgaatat ctggctgagg ccgaggccgg tgcccttgcc
     1441 gaccggcttg gtggtgaaga agggctcgaa cacccgctcc agcacctccg gcggcatgcc
     1501 gctgcccgtg tccgccacgg acaggcggac atagtcgccg gccggaaggg cgttcacctg
     1561 attgtcggcg agcgtgacgt tgtcgacctc gatcctgagc tcgccttcgc cgtccatcgc
     1621 gtcgcggccg ttgacggcga ggttgaggat cgcattttcg agctggtttg tatcgacccg
     1681 gaccggccag ggctcggcgg gcgcgcgcgt gcgcacggtg atccgctcgc cgatcgtgcg
     1741 gtcgacgagg tcgagcatgc cttcgatcag ctcggagggg gcgagattgc tcggcagcag
     1801 cggctcggcg cgggcgaagg cgagcaggcg gcgggtgagg gcggccgctc gggtggcgcc
     1861 ctccatcgca ttgtcgaggt gcatctccac ttcgcgtcgg tcgccgctga gccgccgccg
     1921 ggcaaggtcg aggccgccga ccacgaccgc gagcatgttg ttgaagtcgt gggcgatgcc
     1981 gcccgtcagc tggccgacgg cctccatctt ctgcacctgg cgaagctggg cctcggcggc
     2041 ttcgcgctcg gccgcctcgg agaggaggcg ttcgttggcc gcgctcagct cctgcgtgcg
     2101 ttcccgcacg gcctgctcga gcttgccggc gcggctggac tcgctgtcgg cctcccgcag
     2161 cgcctcgatc cgctcgcgca tggcccggaa ggcgaggaag ccgagggtga gggcgccgat
     2221 gccgatcatc acggcaagcc agccgaacca gtcggtgagc tcgtcggcgc ggtcggaaga
     2281 ggcacgcgtg tcggccatcc ggccgcgcag atcctggcgt tcggcggtgg cgatcccggt
     2341 gagaaggtcc cggacttctc cgccgaggcc ggactggccg gcctggtaga agagcggaat
     2401 gccgccggtc gccttgtgct tcgccgccgc cgccgccgcc gccgcgaacc cgacgccgcg
     2461 ctgctggtag agcgacttca gcttctcgac gcgggcgagc tgctcgggcg tgccggcgac
     2521 gagggagcgc agctgggcga tctggttccc ggcatagacc cagtcc
//
LOCUS       DASVQZ010000134         5961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_414365, whole genome shotgun sequence.
ACCESSION   DASVQZ010000134 DASVQZ010000000
VERSION     DASVQZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414365"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1446)
                     /locus_tag="VF547_03770"
     CDS             complement(223..1446)
                     /locus_tag="VF547_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8261974.1"
                     /translation="MLVAASGNTAMQSLMPAIGRELGVADLWVAVAFSLSAVIWVVTA
                     PHWARRADHRGRRALMRLGLYGFILSALICGGILAAGLHGLISATLVFVIFVFGRAVY
                     GAWGSASPPAVQAYIAARTEGEQRTAYLAALASSFGLGTIIGPAVAPLFIFEPLGLSG
                     PLIVFAAIGVAVLAWVALRLPDDTPHSTARGEVVDYPSMGGFLVGGRERRDGAREGDG
                     SGRLRWRDPRVLPWHVIGTVGGHGHAALLGVIGFLVIDRSGVAPAQSQQSIAIVLMSG
                     AGATLLAQWGLIPFLALGPRRLVVWGLVIAAAGTLLTGFAVSMYGITLGFAVASVGFG
                     LFRPGFTSGASLAVRLDEQNAVAGMVTSVNGVAYIAAPALGVLLYGWWMPLPFVATAA
                     GMVGLAAWTWARVRG"
     gene            1635..2255
                     /locus_tag="VF547_03775"
     CDS             1635..2255
                     /locus_tag="VF547_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8261975.1"
                     /translation="MTFPGEQAQRSRAYARQGAKGGPWLMFLKGFVKHPVMVGSVIPS
                     SKMLIDKMLAPVDWANCKLFVEYGPGVGTFTERVLERLAPDAILLTIDTNADFTKYLS
                     TKIRDSRLRAVTGSAADVRSIMADFGFEEADYILSGLPFSTLPPGVGPAIAEQTAEAL
                     RPGGAFLVYQFSPKVLDFISPYFDRIDRGFEWRNVPPATLFWAWRD"
     gene            complement(2341..2641)
                     /locus_tag="VF547_03780"
                     /pseudo
     CDS             complement(2341..2641)
                     /locus_tag="VF547_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
     gene            complement(2650..3519)
                     /locus_tag="VF547_03785"
     CDS             complement(2650..3519)
                     /locus_tag="VF547_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8261976.1"
                     /translation="MAEVDAERPGLALEVAGNRLTLLADGPERLTALLELVESARSSL
                     RFLYYMFMDDAAGTKVRDALIAAARRGVRVSLLVDGFGSTANRDFFRPLEEAGATFCR
                     FSPKFGRRYLLRNHQKLALADGERVIIGGFNVSDEYFGTVQSGAWRDIGLRVEGDSVG
                     CLMPYFDSLFRWTQTPDARVRDIRRLLQQSSVTQGKLHWLFGGPSRRLSPWARAVRRD
                     MRTARRLDLVAAYFAPSLGMLRRLAGIARRGGRVRIVTASKSDNTATIAAARSTYWWL
                     LRRGISIFVYLPT"
     gene            3638..3979
                     /gene="rpoZ"
                     /locus_tag="VF547_03790"
     CDS             3638..3979
                     /gene="rpoZ"
                     /locus_tag="VF547_03790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX8261977.1"
                     /translation="MARVTVEDCVDKIPNRFDLVLLAAQRARQVSGGAELTIDRDRDK
                     NPVVALREIAEETVKPKQLEEALISGLQRVQVDDDEAPDEVGSLSASAEALRLTAAAP
                     PRNNNAGGDYE"
     gene            3957..4559
                     /locus_tag="VF547_03795"
     CDS             3957..4559
                     /locus_tag="VF547_03795"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8261978.1"
                     /translation="MPAATTNNSRESGYALFDTAIGRCAVVWRGGLVIGAALPETTDE
                     LARTRLARRFPGSAEAEAPPFAASAIARIIGLLAGAKTDLGDIELDLDGSVFERQVWE
                     ATRRIPCGEVRSYGDVARDLGAPQAAQAVGAALGRNPVPIIVPCHRVLAADGGTGGFS
                     APGGLATKFRILEIEGAKRPGEPALFESLPLAVKPAGRTR"
     gene            4556..4993
                     /locus_tag="VF547_03800"
     CDS             4556..4993
                     /locus_tag="VF547_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261979.1"
                     /translation="MIGPAALAAAALAAQGVALPGAGAEWRLATADGDSAIFVDHAGL
                     ERAGNKVRFWSLKVVRRQGPEFSGARALTQADCRKFTYRPLTTVFILGATPVISGAGR
                     TALARPGRSEYEMLRVACGLRAPGAASADPVADMFARWRLEKR"
     gene            complement(5006..5482)
                     /locus_tag="VF547_03805"
     CDS             complement(5006..5482)
                     /locus_tag="VF547_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8261980.1"
                     /translation="MSRAEHLREIFAECGISAALAVVGERWSFLILRGAFNGLRHFEE
                     FQTNLGIARNILSNRLGRLVEHGILRRDPDPADRRKVTYRLTEKGKDLLPVLLSLRQW
                     GERWISGHPSNPVLVDRYTRQPIAPMTVQSADGRPLSLRDMEWVDRAELDRETDAA"
     gene            5593..5907
                     /locus_tag="VF547_03810"
     CDS             5593..5907
                     /locus_tag="VF547_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261981.1"
                     /translation="MNRQLTSTLLAFLFSASLLGGTAFAAPAGQYRAEPAKPPAAARL
                     IVKDIVWRCGASGCVAPRSNSRPAIDCSALAHEIGTLRSFTVEGQALDPAALEKCNAR
                     AR"
BASE COUNT          974 a         2064 c         2076 g          847 t
ORIGIN      
        1 tttcaggagc ggctatctgc acctgccgga ggctaagctc ggcctgctgc agtgatcgag
       61 cggcgactat gtcgccgtcg cggccgctat cgtggcagcg gcatcgttgc cgcgctgttg
      121 gccgcgcaaa tgtgcatcat agaggatgag gccgccttcg tcggtgacgt cgacgctcca
      181 ctcctcgtcc gcccaatgct gcccgcgcga tccctcaatg cactagccgc ggacccgcgc
      241 ccacgtccac gccgccaggc cgaccatccc cgccgccgtc gccacgaagg ggagcggcat
      301 ccaccagccg tagagcagca ctccgagcgc cggcgcggcg atgtaggcga ccccgttgac
      361 cgacgtgacc attccggcga cggcattctg ctcgtcgagc cggacggcga gcgaggcgcc
      421 gctggtgaaa ccggggcgga acaggccgaa gccgacggag gcgacggcga agcccagcgt
      481 gatgccgtac atcgagaccg cgaagccggt cagcagcgtg cctgccgcgg cgatgacgag
      541 gccccagacg acgagccgcc gcgggccgag ggcgaggaag gggatcagcc cccactgggc
      601 gagcagggtc gcgcccgcgc cggacatgag gacgatcgcg atcgactgct gggactgggc
      661 cggggcgact ccggagcggt cgatgacgag gaagccgatg actccgagca gggcggcatg
      721 gccatggccg ccgacggtcc cgatgacgtg ccaggggagc actcgggggt cgcgccagcg
      781 cagccggccg gagccgtcgc cctcgcgggc gccgtcgcgg cgctcgcggc cgccgaccag
      841 gaagccgccc atcgacggat agtcgaccac ctcgccgcgc gcggtcgagt ggggcgtgtc
      901 gtcgggaagg cgaagcgcga cccaggcgag gaccgcgact ccgatcgcgg cgaacacgat
      961 cagcggcccg gacaggccga gcggctcgaa gatgaagagc ggggcgacgg ccggtccgat
     1021 gatcgtcccg aggccgaacg acgacgcgag cgcggcgaga taggcggtgc gctgctcgcc
     1081 ctcggtgcgc gcggcgatat aggcctggac cgccggcggc gacgccgagc cccaggcgcc
     1141 gtagacggcc cggccgaaga cgaagatgac gaagaccagc gtggcggaga tcagcccgtg
     1201 caggccggcg gcgaggatgc cgccgcagat cagcgccgag aggatgaagc cgtagaggcc
     1261 gagccgcatc agcgcccggc ggccgcgatg gtcggcgcgg cgcgcccagt gcggcgccgt
     1321 caccacccag atcaccgccg acagcgagaa ggcgaccgcc acccacagat cggcgactcc
     1381 aagctcgcgg ccgatcgccg gcatcagcga ctgcatcgcc gtgttgcccg acgcggcgac
     1441 cagcatcacg gcgaaaagca gtgcgaatcg ctcccgtgaa agcttggcgg cagggaccat
     1501 ggaggggtaa atcccggaaa tgacgcggtt cgtcccggat taggcggaaa atgtcgtccg
     1561 ggtcgcgccg cccttgccat ccgggcgttg atttgccaac gcacactgct gcaccaggac
     1621 attacggaac catcatgacc tttcccggcg aacaggctca acgatcccgc gcttatgcgc
     1681 gccagggcgc caagggcggg ccgtggctca tgttcctcaa ggggttcgtc aagcatcccg
     1741 tgatggtcgg ctcggtcatc ccctcatcga agatgctgat cgacaagatg ctggcgccgg
     1801 tcgactgggc gaattgcaaa ttgttcgtcg aatacggacc gggcgtcggc accttcacgg
     1861 agcgggtgct ggagcggctc gccccggacg cgatcctgct gacgatcgac accaatgccg
     1921 acttcaccaa atatcttagc accaagatcc gcgactcgag gcttcgggcg gtcaccggct
     1981 cggctgcgga cgtccgctcg atcatggccg atttcggctt cgaggaggcc gactacatcc
     2041 tctcgggcct ccccttctcg acccttcctc cgggcgtcgg cccggccatc gccgagcaga
     2101 ccgccgaggc gctgcgcccc ggcggcgcct tcctggtcta ccaattctcc cccaaggtgc
     2161 tcgacttcat cagcccctat ttcgatcgca tcgaccgcgg cttcgaatgg cgcaacgtgc
     2221 ccccggcgac cttgttctgg gcctggcggg actgatcacg ccgctgtccc cggcgaaggc
     2281 cgcggcccag cttcatcgac cggcgagacc ggggccccgc cttcatccgg gtgctgaccg
     2341 ctacggccga ggcaggccca cctcgcggaa gttgagccgc ttggcgatcc ggtaatcggc
     2401 gaccgcgacg atgaaatagc cgatcgccca gcgcagccgg ttgatcaggg tgcggttgcg
     2461 gccgtggctc gccagcgtca ccgcctgcga atcggcgacc tcgccgtcga cgaagcggcg
     2521 catcgccgcg gcgaagccgg cgtcgtcgac gcgcagcatc atctcgaggt tgaggaacag
     2581 gctgcgcatg tcgaaattcg ccgagccgat atggacgacg tcgtcgacca cgaacagctt
     2641 ggtgtggagc taggtcggca gatatacgaa aatgctgatg ccgcggcgca acagccacca
     2701 ataggtgctt cgagccgctg cgattgtggc ggtgttgtcc gatttcgacg cggtgacgat
     2761 gcgcacccgg ccgccgcgcc gggcgatgcc cgcaagccgc cggagcatgc cgaggctcgg
     2821 cgcgaaatag gcggcgacga ggtcgaggcg ccgggcggtc ctcatgtcgc ggcggacggc
     2881 ccgcgcccag gggctgaggc ggcgcgaggg cccgccgaac agccagtgca gcttgccctg
     2941 ggtgacgctg ctctgctgca gcaggcggcg gatgtcgcgg acccgggcgt cgggggtctg
     3001 ggtccagcgg aacaggctgt cgaaataggg catcaggcag ccgacggaat cgccttcgac
     3061 gcgcaggccg atgtcgcgcc aggcgccgga ctggaccgtc ccgaaatatt cgtccgagac
     3121 gttgaacccg ccgatgatca cccgctcgcc gtcggcaagc gccagcttct ggtggttgcg
     3181 caacaaatag cgtcggccga atttcgggct gaaccggcag aaggtggccc ccgcctcctc
     3241 gagcggccgg aagaagtcgc gattggcggt cgagccgaag ccgtcgacca gcagcgacac
     3301 gcgcacgccc cgccgggccg cggcgatgag ggcgtcgcgc accttcgtgc ccgcggcgtc
     3361 gtccatgaac atgtagtaga ggaagcgcag gctcgagcgg gcggattcga ccagctcgag
     3421 cagggccgtg aggcgctccg ggccgtcggc caggagggtc aggcggttgc ccgccacctc
     3481 cagcgccagg cccggccgct ccgcatccac ttccgccatg gcgggtgcat gtgccgcctg
     3541 cgggcgcagg gcaagccccg tcaaaacatt gacattcgga ccgcccctcc gtatctcgcc
     3601 cgctttcccc ataatggtgt tttgcggagc tgcttgcatg gcgcgcgtta ccgtcgaaga
     3661 ttgtgtcgac aagatcccca accggttcga cctggtgctg ctcgcggccc agcgggcgcg
     3721 gcaggtgtcg ggcggcgccg agctcaccat cgaccgcgac cgcgacaaga atccggtcgt
     3781 cgcgctgcgc gagatcgccg aggagacggt caagcccaag cagctcgagg aggccctgat
     3841 ctccggcctg cagcgagtcc aggtggacga cgacgaggct ccggacgagg tcggctcgct
     3901 gtcggcgtcg gccgaggcgc tgcgcctcac cgccgccgcg ccgccccgca acaacaatgc
     3961 cggcggcgac tacgaataat tcccgggaga gcggctacgc gctcttcgac acggcgatcg
     4021 gccgctgcgc cgtggtgtgg cgcggcggcc ttgtcattgg cgcggccctt cccgagacga
     4081 cggacgagct cgcgcggacc cgcctggcca ggcgctttcc gggttcagcc gaagccgaag
     4141 ccccgccttt cgccgcctcg gcgatcgccc ggatcatcgg cttgctcgcg ggcgccaaga
     4201 ccgatctcgg cgacatcgag ctcgacctcg acggctccgt tttcgaacgg caggtctggg
     4261 aggcgacgcg gcgcatcccg tgcggcgaag tgcgcagcta tggcgacgtc gcgcgcgacc
     4321 tgggcgctcc gcaggccgcc caagccgtcg gagccgcgct cggccgcaat cccgttccga
     4381 tcatcgtccc gtgccacaga gtcctcgccg ccgacggcgg gaccggcggt ttctcggcgc
     4441 ccggcgggct cgccaccaag ttcaggattc tcgagataga gggagcgaag cggccgggcg
     4501 aaccggcact gttcgaatcg ctgccgctgg cagtgaagcc ggccggtcgg actcgatgat
     4561 cggtccggcg gcgctcgcgg cggcggcctt ggcggcgcag ggcgtcgcgc tcccgggggc
     4621 cggggccgaa tggaggctcg cgacggccga cggcgacagc gccatcttcg tcgaccatgc
     4681 cggcctcgag cgggccggca acaaggtgcg cttctggtcg ctcaaggtgg tccgaaggca
     4741 ggggcccgaa ttcagcggcg ccagggcgct gacccaagcc gattgccgaa aattcaccta
     4801 ccgcccgttg accacggttt tcatcctggg ggccacgccg gtcatttcgg gtgcgggaag
     4861 gaccgctctg gcccgcccgg ggcgaagcga gtacgagatg ctgcgcgtcg cctgcggcct
     4921 cagggccccc ggcgccgcca gtgccgatcc ggtggccgac atgttcgccc gctggaggct
     4981 ggaaaagcgc tgacgggggc ggcgatcagg ccgcgtcggt ctcgcggtcg agctcggcgc
     5041 ggtcgaccca ttccatgtcg cgcaggctga gcggccggcc gtcggccgac tggaccgtca
     5101 tcggcgcgat cggctggcgg gtgtagcggt cgaccaggac cggattgctg gggtggcccg
     5161 agatccagcg ctcgccccac tggcgaagcg acagcagcac cggaagcagg tccttgccct
     5221 tctcggtcag ccggtaggtc accttgcggc ggtcggcggg atcggggtcg cggcgcagga
     5281 tgccgtgctc gaccagccgg ccgagccggt tggacaggat gttgcgggcg atgccgaggt
     5341 tggtctggaa ctcctcgaaa tggcgaaggc cgttgaacgc gccgcgaagg atcaggaacg
     5401 accagcgctc gcccaccacc gccagggcgg cggaaatgcc gcactccgcg aatatctccc
     5461 gcaaatgttc cgctcggctc atggggcgac cttagcgttt ttcccgactg taacataaga
     5521 cacaattttc cagttgcaaa ctaaaactga actgcctagt cggttgcata tcgcaactga
     5581 aaggagcccg ccatgaaccg gcaactcact tccaccctgt tggcgttcct cttctccgcc
     5641 tccctgctcg gcggcacggc cttcgcggcg cccgccggcc agtaccgcgc ggagcccgcc
     5701 aagccgccgg ccgcggctcg gctgatcgtc aaggacatcg tctggcgctg cggcgcctcc
     5761 ggctgcgtcg ccccgcgcag caacagccgc ccggcgatcg actgttccgc gctggcccac
     5821 gagatcggaa ccctgcgcag cttcaccgtc gaaggccagg cgctcgaccc ggccgcgctg
     5881 gaaaagtgca acgcgcgcgc ccgctgatga cggaattacg ttgcaacgca gcaccgcccc
     5941 accccatgtt ggggcggtgc t
//
LOCUS       DASVQZ010000135         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_128013, whole genome shotgun sequence.
ACCESSION   DASVQZ010000135 DASVQZ010000000
VERSION     DASVQZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128013"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VF547_03815"
     CDS             complement(<1..529)
                     /locus_tag="VF547_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEX8261982.1"
                     /translation="MPAPAAAVVRLALRHLVDFALPPRCPGCGAITAEPHLFCAGCWR
                     RLALLGDPCCARCGLPFEFGGEGLCGACLADPPPFDRLRAAVAYGDVSRQVALKLKYS
                     GRPGLAGTLAHFMLRHLTDGARQPPLLVPVPLHRWRIWARGYNQAALIVSALERRGGG
                     EAALDLLRRPRATPPL"
     gene            657..2378
                     /locus_tag="VF547_03820"
     CDS             657..2378
                     /locus_tag="VF547_03820"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8261983.1"
                     /translation="MPERVLLLGDDLRAFLAIARSLGRRGVEVHAAPSDFSSPALASR
                     YIAAAHRLPAYPLDPQAWEAALARLIRQHDFRRVVPTSDSGLFMLRHHADALGRERLG
                     IVSETALAIFTDKANTRALAEANGVDVARGRLIGGEDSAETLAAALGLPLVLKPRTSY
                     ALGDLKTKRSARVVRDSADLAAQLGSGAWAGCVAESFFPGVGVGVSVLAREGRILLAY
                     QHRRLHESSETGASTRRVSEPVDPSMLAAVVPLAAAAKLDGAAMFEFRLDRRSGRHVL
                     LEVNPRFWGSLPLAVAAGADFPGLWWDLAVHGREGTTGYRPGVVKADLTGEFDRAVDR
                     LEAARGLGRLRAAFEGLGAATALFFAREGADSWALDDPEPWRSERRQLLGRLRDALRK
                     RLPGRKRRNRVRFEDVAQTIAERAQARPVRLIVVGRDNVCRSAFAERLLRLRLAGLPV
                     EIASAGHVPRRGARPSEATVTAAAEFGIDLSGHRSAMLSVADLERADVLILLDEDCEW
                     RLRQLHPDFTAERVTLAEPGSGAQAFPALAAALERLAGVVEQSFRPAPRAPASGGEAQ
                     PIRRQAA"
     gene            2389..3882
                     /locus_tag="VF547_03825"
     CDS             2389..3882
                     /locus_tag="VF547_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8261984.1"
                     /translation="MSEPTPRNALYRSALLAVAMQWTLRFIGLISVFILARLLHPSDF
                     GIMGLAVAALALVELLGAVGLWQALLRIKEWDREHLDTAWTIQLIVFGTMGLIALVTA
                     PLVARFFGQPALTAVLAVLAVRYLVLGLANIGVVEFDRKLAFGRDLRMRVAARLASFV
                     IAVSAAFWLRNYWALVIGLVSQNICYSIATYIAHPFRPRLSLKRRAELLGTSMWIFVH
                     SVTQTVQMQIERLVMGRFSSPHIIGLYSVSKDLSEIFTQEIATALNRVTFVTVAKTGQ
                     PLSETPLTTAQILGAYAMIAAPMGLGLAATAEPAIHLLLGAEWTEAAPFLTIVAVYSA
                     LYTVYKVIASSLQAAGHARQAAFMSAGGALCLAVTVAAAGWLWPSAMAVAIAAFGANF
                     LVLVSGIAVMAAYSGQNAFALGLHVFRPFAAAAAMAATVRLIGPDSGSAIVDLIGGVA
                     VGTAAYPLLLLGLWWASGRPAGAEREAVLVLAEMRARLRRRPAAGKA"
     gene            3886..>4106
                     /locus_tag="VF547_03830"
     CDS             3886..>4106
                     /locus_tag="VF547_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261985.1"
                     /translation="MELLVRLSDWIYLALNGLAGRSWLLDTMIALPLDNNLVKAGPIG
                     ACFVYAWWSGKDEADTRRRRGTLLVTIASL"
BASE COUNT          518 a         1522 c         1466 g          600 t
ORIGIN      
        1 gcagcggcgg cgtcgcccgg ggtcggcgca gcaggtcgag cgcggcctcg ccgccgccgc
       61 ggcgctccag cgccgagacg atcagagccg cctggttgta gccgcgagcc cagatccgcc
      121 agcggtgcag cgggaccggg acgagcagcg gcggctgccg ggcaccgtcc gtgagatggc
      181 ggagcatgaa atgggcgagc gtcccggcga ggcccgggcg gccgctgtat ttgagcttga
      241 gcgcgacctg ccgggagacg tcgccatagg cgacggcggc gcgaaggcgg tcgaagggcg
      301 gcggatcggc gaggcaggcg ccgcacagcc cttcgccccc gaattcgaac ggaaggccgc
      361 agcgcgcgca gcaggggtcg ccgagcaggg cgagcctgcg ccagcagccg gcgcagaaga
      421 ggtgcggctc ggccgtgatc gcgccgcagc ccggacagcg cggcggaagc gcgaaatcga
      481 cgaggtggcg gagcgcgagt cgaacgacgg cggcggccgg ggcgggcatc gggcgattgt
      541 gacccgcggg gcggcgcgac tcaatccgcc gcgggcgaat tcttccccgc ccgttaggga
      601 tatttgaacg gcctgcggcg catagccccg cccgaaggac gaggcgaacg aggacgatgc
      661 cggaacgcgt gctgctgctg ggcgacgacc tgcgcgcctt cctcgccatt gcgcgctcgc
      721 tcgggcgccg cggcgtggag gtccatgccg ctccgtccga tttctcctcg ccggcgctgg
      781 cctcgcgcta catcgccgcc gcgcaccggc tgcccgccta tccgctcgac ccgcaggcct
      841 gggaagcggc gctggcgcgg ctgatccgcc agcacgattt ccgccgggtg gtgccgacca
      901 gcgacagcgg actgttcatg ctccgccacc atgccgacgc gctcggtcgc gagcggctcg
      961 ggatcgtcag cgagacggcg ctcgccatct tcaccgacaa ggccaacacc cgcgccctcg
     1021 ccgaggcgaa cggagtcgac gtcgcgcggg gaaggctgat cggaggcgag gacagcgcgg
     1081 agacgctggc ggcggcgctg ggcctgccgc tcgtgctcaa gccccgcacc tcctacgcgc
     1141 tcggcgacct caagaccaag cgctccgccc gggtggtgcg cgactccgcc gacctcgccg
     1201 cgcagctggg ctccggcgcc tgggccggat gcgtcgccga gagcttcttc cccggagtgg
     1261 gcgtcggcgt ctcggtcctc gcccgcgagg gaaggatcct cctcgcctat cagcatcggc
     1321 ggcttcacga gagctccgag accggtgcca gcacccggcg ggtgagcgag ccggtcgatc
     1381 cgtccatgct cgccgcggtc gttccgctgg ccgcggcggc gaagctcgac ggcgccgcga
     1441 tgttcgagtt ccggctcgac cggcgcagcg gccgccacgt cctgctcgag gtcaatccgc
     1501 gcttctgggg ctcgcttccg ctggcggtcg cggcgggcgc cgatttcccc ggcctgtggt
     1561 gggacctggc cgtccacggc cgcgaaggca cgaccggcta caggcccgga gtggtcaagg
     1621 ccgatctcac cggcgagttc gaccgcgccg tcgaccgcct cgaggccgcc cgcggattgg
     1681 gccgcctccg agcggcgttc gaaggcctcg gcgccgcgac cgcgctgttc ttcgcccgcg
     1741 agggcgccga ttcctgggcc ttggacgacc cggagccgtg gcgctcggag cggcggcagc
     1801 tgctcggccg cctccgcgac gcgctgcgca agcgcctccc cggccgcaag cgccgcaacc
     1861 gagtccggtt cgaggacgtc gcccagacga tcgccgaacg ggcgcaggca cggccggtcc
     1921 ggctgatcgt ggtcgggcgc gacaacgtct gccgaagcgc cttcgccgag cggctgctga
     1981 ggctgaggct tgcgggcctc ccggtcgaga tcgcctcggc cgggcacgtc ccgaggcggg
     2041 gcgcccgccc ttccgaagcg acggtgacgg ccgcggccga gttcgggatc gacctttccg
     2101 gccaccgctc cgcaatgctg tccgtggccg acctcgaacg ggccgacgtc ctgatcctgc
     2161 tcgacgagga ttgcgaatgg cggctgcgcc agctgcaccc cgacttcacc gccgagaggg
     2221 tgaccctggc cgaaccggga tcgggcgccc aggccttccc agccctcgcc gccgcgctcg
     2281 agcggctggc cggcgtcgtc gagcagagct tccggccggc gccgcgcgcg cccgcctcgg
     2341 gcggcgaagc ccagccgatc cggcggcagg ccgcgtgagg accggccgat gagcgagccg
     2401 actccgcgca acgcgctcta ccgaagcgcc ctgctcgccg tggcgatgca atggaccttg
     2461 cgcttcatcg ggctgatctc ggtgttcatc ctcgcccgcc tgctccatcc cagcgacttc
     2521 gggatcatgg gcctcgccgt ggcggcgctg gcgctggtcg agctgctggg ggccgtcggc
     2581 ctgtggcagg cgcttcttcg gatcaaggaa tgggaccggg agcatctcga cacggcctgg
     2641 acgatccagc tgatcgtgtt cggcacgatg gggctgatcg cgctcgtcac cgccccgctc
     2701 gtcgcccgct tcttcggcca gccggcgctg accgccgtgc tggcggtgct ggcggtccgc
     2761 tacctcgtgc tcgggctcgc caacatcggc gtcgtcgagt tcgaccgcaa gctcgccttc
     2821 ggccgggacc tcaggatgcg ggtcgccgcc cggctcgcct cgttcgtcat cgccgtctcc
     2881 gccgccttct ggctgcgcaa ctactgggcg ctcgtgatcg gcctcgtctc ccagaacatc
     2941 tgctattcga tcgccaccta catcgcccac ccgttccggc cgcggctgtc gctcaagcgg
     3001 cgggccgagc tgctcgggac ctcgatgtgg atcttcgtcc attcggtgac ccagaccgtt
     3061 cagatgcaga tcgagcggct ggtcatgggc cggttcagca gcccccacat catcggcctc
     3121 tattcggtct cgaaggacct gtcggagatc ttcacccagg agatcgccac cgccctcaac
     3181 cgcgtcacct tcgtcaccgt cgccaagacc ggccagccgc tcagcgagac cccgctgacg
     3241 accgcccaga tcctgggcgc ctatgcgatg atcgcggcgc cgatgggcct gggcctcgcg
     3301 gcgaccgcgg agccggccat ccacctgctg ctcggcgccg agtggacgga agcagcgccc
     3361 ttcctcacga tcgtcgccgt ctattcggcg ctctacacgg tctacaaggt gatcgcctcg
     3421 tcgctgcagg cggccggcca tgcgcgccag gcagccttca tgtcggcggg gggcgccttg
     3481 tgcctcgccg tcacggtggc ggcggcgggg tggctgtggc cgtccgccat ggcggtcgcg
     3541 atcgccgcgt tcggagccaa tttcctcgtc ctcgtcagcg ggatcgcggt catggccgcc
     3601 tattcgggcc agaacgcctt cgcgctcggc ctccatgtct tccggccgtt cgccgccgcg
     3661 gccgcgatgg cggcgacggt ccggctgatc ggtccggaca gcggcagcgc gatcgtcgac
     3721 ctgatcggcg gagtcgcggt cgggaccgcc gcctaccccc tgctcctcct cggcctgtgg
     3781 tgggcgagcg gccggccggc cggcgccgag cgcgaggccg tgctggtcct cgcggagatg
     3841 cgggcgcggc tgcgccgccg cccggcggcg gggaaggcct gagccatgga gctgctcgtc
     3901 cgcctcagcg actggatcta cctcgcgctg aacggcctcg ccggccgctc ctggctgctc
     3961 gacacgatga tcgcgctccc cctcgacaac aatctggtga aggcgggccc gatcggcgcc
     4021 tgcttcgtct atgcctggtg gtcgggcaag gacgaggccg acacccggcg ccgccgcggg
     4081 accctgctcg tcacgatcgc ctccct
//
LOCUS       DASVQZ010000136         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_414611, whole genome shotgun sequence.
ACCESSION   DASVQZ010000136 DASVQZ010000000
VERSION     DASVQZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414611"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF547_03835"
     CDS             complement(<1..400)
                     /locus_tag="VF547_03835"
                     /inference="COORDINATES: protein motif:HMM:NF021025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HEX8261986.1"
                     /translation="MARTKSLAALVSIGAALALPSVAGAKPPERHDARRGLDSLNQPV
                     VQRTDYLLDLADNGGLSDSDLGRLDSWFASLRLGYGDHVFVDRGTDRGRQDVAGVAAA
                     YGLLMSPGAPVLEGDVPMGAVRVIVSRTTAS"
     gene            complement(421..2028)
                     /locus_tag="VF547_03840"
     CDS             complement(421..2028)
                     /locus_tag="VF547_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX8261987.1"
                     /translation="MTKLNMMRRATVGTAIISVLAASVTTVTPSVALAAQPSAGTYRP
                     TNQVLLSVGEGEVISLPRSVVDVWTSNPAVADVNVTSPRQLGLFGKDAGEATVIATAA
                     DGSIVYGARVRVSQNISSVDQMLKAAMPDANISITHVGQIAVMNGTVASPADSAQAEA
                     LVRTMLNPGVDANAAGAVLRVTPVNRLKTATPLQVMLQVRIAEVSRSLTRDIGVNLAS
                     ADSTGGFKFGIGQGRTVTPKYTPGGPIGTGSEASEGATAVVANTIGSTLFGAGKLFGV
                     DILGALDLAEQNGKVHTLAEPNLVALSGETASFLAGGEFPIPSSNGINGTSIEFKQYG
                     VSLAFTPYVLEGGRISMRVRPEVSELSDAGAIQINGFRVPSLTTRRTETTVELGSGQS
                     FMIGGLLSNSSSNNIKQAPILGSLPIIGALFRSTGFRRNETELVIVVTPYLVKPVNAS
                     QIALPTDGFRSANTAERTFLGTAEYNKPVPRPGPTMAEPRTASPGLGGVASVPARRLP
                     AAPAPARRAEAKATRPQTASATPGFSF"
     gene            complement(2028..3092)
                     /gene="cpaB"
                     /locus_tag="VF547_03845"
     CDS             complement(2028..3092)
                     /gene="cpaB"
                     /locus_tag="VF547_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8261988.1"
                     /translation="MDVKKLVLLIGALIVAAVTAVTAKNMFSGAGAPSASAKPSVPAG
                     PEVLVATRALPVGTIIDAEAFRFQRWPEGLVQPAYYIKGKPGVRPADLVGTVVRNEIT
                     AGQPFTQGSLIRPGDRGFLAAALGPGMRAVTVAVSATSGVAGFVFPGDRVDIVLTQEI
                     EGGGDGTPLKVSETVMRNVRVLATDQRMSAQGEDGKAVVQRFSMVTLEATPKIAEKIA
                     VAQKIGDLSLSLRSIADNSAELERAIASGEVTVPVTDDPKAERRMLLAVANQPVDGGS
                     TYTTGSDVSRFQRSTVPGSGKAKPSSDDKIADAISAMALASAGRAPAAAAGGEAPTSK
                     GPQVRVARGNSVTIVPVGAK"
BASE COUNT          455 a         1018 c         1163 g          529 t
ORIGIN      
        1 cgctcgccgt ggtccggctg acgatcaccc ggaccgcgcc catcggcacg tcgccctcga
       61 ggacgggtgc tccggggctc atcaggaggc cgtaggccgc cgcgactccg gcgacgtcct
      121 ggcggccgcg gtcggttccc cggtcgacga agacatggtc gccatagccc aatcgaagcg
      181 aggcgaacca ggagtcgagc cggcccagat ccgagtcgct gaggccgcca ttgtcggcga
      241 ggtcgagcag atagtcggtg cgctggacga ccggctggtt gagcgagtcg agaccccgcc
      301 tcgcatcgtg ccgttccggc ggcttcgctc cggcgacgga ggggagggcg agtgcggcgc
      361 cgatcgagac gagggcggcg agagacttgg tgcgtgccat gatcacgtcc tttcgatcgg
      421 tcagaagctg aagccgggag tggcggaggc ggtctgcgga cgggtcgcct tcgcttcggc
      481 ccggcgcgcg ggcgccggag ccgcgggaag gcggcgcgcc ggaacgctcg ccactccgcc
      541 gaggccgggg ctcgcggtgc gcggctcggc catggtcgga ccggggcggg gcaccggctt
      601 gttatattcg gccgtgccga ggaaggtccg ctccgccgtg ttcgcgctcc ggaaaccgtc
      661 ggtcgggagc gcgatctggc tcgcgttgac cggcttcacc agatagggcg tgacgacgat
      721 caccagctcc gtctcgttgc ggcggaagcc ggtcgagcgg aacagggcgc cgatgatcgg
      781 aaggctgccg aggatcggcg cctgcttgat gttgttgctg ctgctgttgc tgagcagtcc
      841 gccgatcatg aagctctggc cggagccgag ctcgaccgtg gtctccgtac gccgggtggt
      901 cagcgagggg acccggaagc cgttgatctg gatcgcgccg gcatcggaaa gctcggacac
      961 ttccggacgg acgcgcatcg agatccggcc gccttcgagc acgtaaggcg tgaaggcgag
     1021 gctcactcca tattgcttga actcgatgga cgtaccgttg atcccgttgg aggacgggat
     1081 cgggaactcg ccgccggcca ggaagctggc ggtctcgccc gaaagggcga ccaggttcgg
     1141 ctcggcgagc gtgtggacct tgccgttctg ctccgcgaga tcgagcgctc ccaggatgtc
     1201 gaccccgaac agctttccgg cgccgaagag ggtggagccg atcgtgttgg cgacgacggc
     1261 ggtggcgccc tcggacgctt ccgagccggt tccgatcggg ccgccgggcg tgtatttcgg
     1321 agtgacggtc cggccctggc cgatcccgaa cttgaagcct ccggtggaat cggcggaggc
     1381 gaggttgact ccgatgtcgc gggtgagcga gcggctcacc tcggcgatcc tcacctgcag
     1441 catcacctga agcggagtcg ccgtcttgag ccggttgacc ggggtcaccc ggagaacggc
     1501 cccggccgcg ttggcgtcca ctccggggtt cagcatggtc ctcacgagcg cttccgcctg
     1561 ggcgctgtcg gcgggcgatg cgaccgttcc gttcatcacc gcgatctggc cgacatgggt
     1621 gatcgagatg ttggcgtccg gcatcgcggc cttcagcatc tgatcgaccg agctgatgtt
     1681 ctggctgacc cggacccggg cgccgtagac gatcgagccg tcggcggcgg tggcgatcac
     1741 cgtcgcctcg ccggcatcct tgccgaacag tccgagctgg cgcgggctgg tgacgttgac
     1801 gtcggcgacc gccgggttgg aggtccagac gtcgaccacg ctcctcggca gcgatatgac
     1861 ttcgccttcg ccgaccgaga gcagcacctg gttggtcggg cggtaggtgc ccgctgaggg
     1921 ttgcgcggcg agggccacgc tcggcgtcac ggtggtgacg cttgcggcga gtaccgagat
     1981 tatcgcggta ccgaccgtcg cacggcgcat catgttcagc tttgtcatta ttttgctccg
     2041 accggaacga tggtgacact gttgccgcgg gcgaccctga cctggggacc cttggaagta
     2101 ggcgcctcgc cgccggctgc ggcggctggg gcgcgtcccg ccgaggccag cgccatggcg
     2161 ctgatcgcgt cggcgatctt gtcgtcggac gagggcttgg ccttgccgct gccggggacg
     2221 gtgctgcgct ggaagcgcga gacgtcggag ccggtcgtgt aggtgcttcc tccgtcgacc
     2281 ggctggttgg cgacggcaag cagcatccgc cgctcggcct tcggatcgtc ggtgaccggg
     2341 accgtcacct cgccggaggc gatggcgcgc tcgagctcgg ccgaattgtc ggcgatcgag
     2401 cggagcgaca gggacaggtc gccgatcttc tgggcgaccg cgatcttctc cgcgatcttg
     2461 ggggtcgcct cgagggtcac catggaaaag cgctggacca cggccttgcc gtcctcgccc
     2521 tgcgcggaca tgcgctggtc ggttgcgagc acccggacgt tgcgcatcac cgtctcggag
     2581 accttgaggg gcgttccgtc gcctccgcct tcgatctcct gggtgaggac gatgtcaacc
     2641 cggtctcccg ggaagacgaa gccggccact ccgctggtcg cggagacggc gacggtgacc
     2701 gccctcatcc ccggcccgag cgcggcggcg aggaagccgc ggtcgcccgg cctgatcagc
     2761 gagccctggg tgaagggctg gccggcggtg atctcgttgc ggacgaccgt cccgaccagg
     2821 tcggccgggc ggacgccggg cttgcccttg atgtagtaag cgggctggac gaggccttcg
     2881 ggccagcgct ggaagcggaa ggcttccgcg tcgatgatgg tgccgacggg gagggcgcgg
     2941 gtcgcgacca gcacttccgg ccctgcgggc acggagggct tggccgaggc cgaaggtgcg
     3001 ccggctccgc tgaacatgtt cttggcggtc accgcggtga cggccgcgac gatcagcgct
     3061 ccgatcagca gcacgagctt tttcacatcc atgacgctag tcgcctcctc gcccgctcaa
     3121 aattgcaggc tcccacatca gacaaattgg ttaagaaacc gttcg
//
LOCUS       DASVQZ010000137         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_224818, whole genome shotgun sequence.
ACCESSION   DASVQZ010000137 DASVQZ010000000
VERSION     DASVQZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224818"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VF547_03850"
     CDS             <1..256
                     /locus_tag="VF547_03850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665622.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX8261989.1"
                     /translation="HHFKLYTEGFHVPEGEVYVATESPKGEFGVYLVADGTNKPYRVK
                     IRPTGFSHLQAMDFMTRGHMLADVTAILGSLDIVFGEIDR"
     gene            334..783
                     /locus_tag="VF547_03855"
     CDS             334..783
                     /locus_tag="VF547_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261990.1"
                     /translation="MPPPPHNPLMARCAIRKVADAINARAVDGLIAKAKVYTDLLGEV
                     GPEEEGSFLDLFGKEAELNQQENLRVKDVSLLTTRGLYVAVFDRYHKEAAKRSDRYMK
                     SHWSVWLIAFESNEIVSMRQATELWPFTLQNPAFGSDEVCPMELKGG"
     gene            776..1465
                     /locus_tag="VF547_03860"
     CDS             776..1465
                     /locus_tag="VF547_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX8261991.1"
                     /translation="MADLLPTSIDLDELRARWGSFAFTAENDRQAKTIVGRYPPGRQQ
                     SAIIPLLDLAQRQVGAETGTQGWLPVPVIEYVAKYLGMPYIRAYEVATFYTMFNLRPV
                     GRFHVQVCGTTPCMLRGSDDVFAACKAKGMKKGQTTPDGLFTLSEVECLGACANAPMA
                     QINDDNYEDLTFETTTALLEALARGETPKPGPQGVNRRTSEPEGGVTSLHAMVTENHD
                     YRPRWKEGSVG"
     gene            1574..>2562
                     /gene="nuoF"
                     /locus_tag="VF547_03865"
     CDS             1574..>2562
                     /gene="nuoF"
                     /locus_tag="VF547_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX8261992.1"
                     /translation="MLDDKDRIFTNLYGFQDWGLKGAQARGDWDDTKALMAIGEDAII
                     DEIKNSGLRGRGGAGFPTGMKWSFMPKAPTPGRPNFLVINADESEPGSCKDREIIRHD
                     PHKLIEGALIAGFAMRARAGYIYIRGEFIREAQTLEAAVRQAYDAGLLGRNAARSGYD
                     FDLFVHRGAGAYICGEETAMLESLEGKKGMPRLKPPFPAGAGLYGCPTTVNNVESIAV
                     VPTILRRGAAWFKSFGREKNEGTKLFQISGHVNRPCVVEDAMSVPFRELIERHAGGIR
                     GGWDNLLAVIPGGSSVPLVPAHEIIDAPMDFDTLRNLKSGLGTAAVMVLDKST"
BASE COUNT          470 a          822 c          844 g          426 t
ORIGIN      
        1 ccaccacttc aagctctaca ccgagggctt ccacgtcccc gagggcgagg tctatgtcgc
       61 caccgagagc cccaagggcg agttcggcgt ctatctcgtc gccgacggca ccaacaagcc
      121 ctaccgggtc aagatccgcc cgaccggctt ctcgcacctg caggcgatgg acttcatgac
      181 caggggccac atgctcgccg acgtcaccgc catattgggc tcgctcgaca tcgtcttcgg
      241 ggagatcgac cggtgacgag cgcgtcttgg cgagcggccg tcctgtcggg tctcgcattg
      301 gcctgggtcg gtccggtctg cgcaactacg cccgtgccgc cgcctcccca caacccacta
      361 atggcgaggt gcgcgatccg gaaagtcgcc gacgcaataa acgcaagggc ggtcgacgga
      421 ctcatcgcca aggccaaagt atacaccgat ttgcttggcg aggtaggtcc ggaggaagaa
      481 ggatcatttc ttgatctatt cggcaaagag gcggagctga atcagcagga aaacttgagg
      541 gtcaaggatg tatcgctttt gactaccaga ggcctgtacg tggccgtctt tgatagatac
      601 cataaggagg cggcgaagcg tagcgacaga tacatgaagt cgcattggtc ggtctggctt
      661 atcgctttcg agtcaaatga gatcgtttcg atgcgtcaag caacggagct ctggcccttt
      721 acccttcaga atcccgcatt cggatcggat gaggtttgtc cgatggagct gaagggtggc
      781 tgatcttctc ccgacttcga tcgatctcga tgagctgcgc gcccgctggg ggtcgttcgc
      841 cttcacggcg gagaacgacc gccaggcgaa gacgatcgtc ggccgctatc cgccgggccg
      901 gcagcagagc gcgatcatcc cgctgctcga cctcgcccag cgccaagtcg gggcggagac
      961 cgggacccag ggctggctgc ccgttccggt gatcgaatat gtcgcgaagt atctcggcat
     1021 gccctatatc cgcgcctacg aggtcgcgac cttctacact atgttcaacc tgcgccccgt
     1081 cggccgcttc cacgtccagg tgtgcggcac cacgccgtgc atgcttcgcg gctcggacga
     1141 cgtcttcgcc gcctgcaagg ccaaggggat gaagaagggc cagaccactc cggacggcct
     1201 cttcaccctc tcggaggtcg agtgcctggg cgcctgcgcc aacgcgccga tggcccagat
     1261 caacgacgac aattacgagg acctcacctt cgagacgacg accgccctgc tcgaggcgct
     1321 ggcgcgcggt gagaccccga agccgggccc gcagggggtg aaccggcgga cgagcgagcc
     1381 ggaagggggg gtgacgagcc tgcacgcgat ggtgacggag aatcacgact accggccgcg
     1441 ctggaaggag ggctcggtcg gatgagcatc ttcgtcatgc cagcgaaagc tggcatccat
     1501 ggggctgccg agccccgcat cgagtcggca tggaccccag ctttcgctgg ggtgacggcc
     1561 tgtggggccc gtcatgcttg acgacaagga ccgcatcttc accaacctct acggcttcca
     1621 ggactggggg ctgaaggggg cgcaggcgcg cggcgactgg gacgatacca aggcgctgat
     1681 ggcgatcggc gaggacgcca tcatcgacga gatcaagaac agcggcctgc gggggcgggg
     1741 cggcgccggc ttcccgaccg ggatgaaatg gtcgttcatg cccaaggcgc cgacgccggg
     1801 ccggcccaat ttcctcgtca tcaacgccga cgaatccgag cccggctcgt gcaaggaccg
     1861 cgagatcatc cgccacgatc cgcacaagct gatcgagggc gccctgatcg ccggcttcgc
     1921 gatgcgggcg cgggccggct acatctacat ccgcggcgag ttcatccgcg aggcgcagac
     1981 gctcgaggcg gcggtccggc aggcctatga cgcggggctg ctcggcagga acgcggccag
     2041 gtcgggctat gatttcgacc tgttcgtcca tcgcggcgcc ggcgcctaca tctgcggcga
     2101 agagacggcg atgctcgaga gcctcgaggg caagaagggt atgccgcggc tgaagccccc
     2161 cttcccggcg ggggccggcc tctacggctg cccgaccacg gtcaacaatg tcgaatcgat
     2221 cgcggtggtg ccgaccatcc ttcgacgcgg cgccgcctgg ttcaagtcgt tcgggcgcga
     2281 gaagaacgaa ggcaccaagc tcttccagat ctccgggcac gtgaacaggc cgtgcgtcgt
     2341 cgaggatgcc atgtcggttc cgttccggga gctgatcgag cgtcacgccg gcggaatccg
     2401 cggcggctgg gacaatctgc tcgcggtcat tcccggcggc tcgtccgtgc cgctcgtgcc
     2461 tgcgcacgag atcatcgacg cgccgatgga tttcgacacg ctccggaacc tgaaatcagg
     2521 cctcggcacc gcggccgtga tggtgctcga caagtcgacg ga
//
LOCUS       DASVQZ010000138         8523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_29172, whole genome shotgun sequence.
ACCESSION   DASVQZ010000138 DASVQZ010000000
VERSION     DASVQZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29172"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VF547_03870"
     CDS             <1..1069
                     /locus_tag="VF547_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8261993.1"
                     /translation="AAAEIAAALEAAASGDAAARARAELLLSRAYPAFVRDLRVPRSG
                     AAAVTWVDRELAPQAPDPRALLEAAAAAPDLGRHLAEAMAVNPLYAALSRGYARRAAS
                     RGLTAAEEKVVRANLDRARLIPHRPGRYVIVDAGSARLWMIDGDRVEGPMRVIVGKPD
                     MQTPAMAGLIRYVTLNPWWNVPPDLARSRARRVLRSGPGFLARERMELLSDWGDQPRA
                     VTAAQVDWKAVAGGRTSLRMRQRPGGDNVMGAMKFMLPNRLGIYLHDFPDKSLFARAD
                     RRLSSGCVRLADAPRLARFLFGGAVPRPAGPAPEQRVDLPEPVPVYITYLTALPDPEK
                     GVVLQRDVYARDGRRSSRRSA"
     gene            1114..1857
                     /locus_tag="VF547_03875"
     CDS             1114..1857
                     /locus_tag="VF547_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261994.1"
                     /translation="MRRSGPAVLPLLLLAGCAGYAADYWKPKGNLIAPQLPRYGISAE
                     DSRCVGERLTKNLSVWQLRQLADIARRLVPREGGALAPRDLAYVAGLVQDPKVGPEVA
                     ATLAACNVGQAAPGPTAASTATPNPAASQPGLVPGATPPQPLRWIDLGAAETGQRIAV
                     DISTVVMNAEKREGWFRLSNPGQTSPSADAYRLRVDCPGRTITPTAARKYDSDGKVVQ
                     QEDYTAAWQQPLPIEAGTVMEIAWKRLCE"
     gene            1984..2145
                     /locus_tag="VF547_03880"
     CDS             1984..2145
                     /locus_tag="VF547_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261995.1"
                     /translation="MEQRHRTEDVGHYTRLEAGARWLAGRAAGGAERDLHLGHARRYA
                     RLRADAGGF"
     gene            2147..2338
                     /locus_tag="VF547_03885"
     CDS             2147..2338
                     /locus_tag="VF547_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8261996.1"
                     /translation="MTGLPRRPTTVERAFELARSGACANLPAIAAALKGERHDSVDAH
                     LAGPSIRKDLRRVWEAAAQ"
     gene            complement(2357..3580)
                     /locus_tag="VF547_03890"
     CDS             complement(2357..3580)
                     /locus_tag="VF547_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HEX8261997.1"
                     /translation="MVSPFAVHHPQAAHVVIELFGGDNNLTDYVEEDMAEMAAGIGED
                     VAVLAVADVFNGPARVVEITARHGVSVVEELGEIDTGDPEVLSELLGRALASFSETSR
                     IAIGFWDHGSGVFDERGLLRRVLPRARLPRWRLSRSFPARRLFFSTAELLHDVELRAM
                     LHDDQSGGLLTNREAGNMLRDSFAAAGRKRPVDLIFSDTCLNGMIEVASEFEEFAQCI
                     VGSEELEPGDGWDYHEWFSRMAASPPADGQAWGRQAVEAMEAGYAGRPEQHPVTLSAV
                     RSRSGVADAFADLIRVASPLGRPGFRYLDWARSQSQIFASGYDSYDMLDFAEKLAADG
                     EIAEIAAAAEGLAGAIREAVVHSIALGAAVARAKGLAFWFPASRGSFDKDVETYADLR
                     FDGLTGWSTYLDGHR"
     gene            3996..4634
                     /locus_tag="VF547_03895"
     CDS             3996..4634
                     /locus_tag="VF547_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8261998.1"
                     /translation="MITVTAFRSVPPFARGLVRDLRVRWALEEAGLRYAVKLLAEGEK
                     DGAEYRAWQPFGQVPAYEENGLKLFESGAIVLRIAERSETLLPGDPAARARAAQWLFA
                     ALNTIEPPILNLAGIDLFHADQEWARLRRPDAEAAVRNRLGELAAALGDKPFLDGPRF
                     TAGDLMMATVLRILRHTGLVAEQPRLAEWLARCESRPAFERALAGQLADLDA"
     gene            complement(5054..6025)
                     /locus_tag="VF547_03900"
     CDS             complement(5054..6025)
                     /locus_tag="VF547_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8261999.1"
                     /translation="MVRWGMIGCGAVTERKSAPAYAQAAGSRLVAVASRRPEAARAYA
                     GRRGIGLVFDDPDALIRSPEVDAVYIATPPSSHRALAAKVAAAGKPCCVEKPIALSHA
                     EAAAMVDAFRAAGQPLFVAYYRRSLPRFAQVRRWIAEGAIGEVRHVHWALARAPTPGD
                     LSGDPGWRADPAEAPGGYFEDLACHGLDLFDFLLGPIDEAAGIHRNRAGLYAVPDSVA
                     AAWSHSGGATGSGFWTFAACRVEDEVRLLGSEGRISFSVFEEAPLVLETGAGGRTVEI
                     PNPDPIQLHHVENMIRHLSGEAPHPSTGESAARTAWVTDRIFARTAG"
     gene            6490..>8523
                     /locus_tag="VF547_03905"
     CDS             6490..>8523
                     /locus_tag="VF547_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8262000.1"
                     /translation="MRAFTDALLPGEAQSPGAAELEVAEFLDDFVGASPAGEAELWRS
                     GLKALDDRARSDFGRAFADLGEAELGELLTALLDPAAAPAPLADFARRAKAAAIRAYY
                     RTEHGLHGELKVARPAQAAPSAAFEGGFPEPDRSRTRYDVVIVGSGATGGWAAKELTE
                     AGFEVLVLDSGGMPPPAGQVRQPYQVRFRNMFDNMTLNGARQPVQARSWACDEYRSAL
                     FIDDREHPYEGADGFTWIRASSVGGRMNMWGRQVYRYSRQDLAASNMEGRSEPWPLDY
                     DELAPFYDEVERFIGVSGALDGCEHLPDGKFLPPMAMTDGEKHLKQAVESRWPDRRVI
                     IGRTATLTRDHEGRKACDGAGHCYRGCPTRSFFEPLGSTLASARATGRLHLMPKAHVL
                     RLVMDEEKGEARGVAYVDLRDGSEHLVEARAVVLAASTIASTRILMSSRTPRLPDGVG
                     SSSGVLGAYLHGHIHSVMCSGQVPWLLKPAGMHDEGRPNQIYIPQFLNNAGQKPEPGF
                     VGGFGVEGAVKHYMFPRDMAVRPGFGRDLKRSIREDRVPAHFFLTAFGVMLARPENRV
                     ELSGSTDKWGVPTIKVSCGYGDNDLRMAEAMAKSVVEIAEAADFTVSSVSPAPGLPGM
                     CVHEVGTARMGSDASSSVLDPWNRVWDSPNVLVVDGACFPSSGPQNPTLTMMAL"
BASE COUNT         1252 a         2989 c         3103 g         1179 t
ORIGIN      
        1 cgccgccgcc gagatcgccg ccgcgctgga agcggcggcc tccggcgacg ccgccgcccg
       61 cgcccgcgcc gaattgctgc tgtcgcgcgc ctatccggcc ttcgtccgcg atcttcgagt
      121 gcccaggagc ggagcggccg cggtcacctg ggtcgaccgc gagctcgccc cgcaggcccc
      181 ggatccgcgc gccctgctcg aggcggcggc ggcggcgccc gatctcggcc gccacctggc
      241 cgaggcgatg gcggtcaacc cgctctacgc ggcgctgagc cggggctacg cccgccgagc
      301 cgcctcgcgc ggcctcaccg ccgccgagga aaaggtggtc cgcgccaatc tcgaccgggc
      361 ccgactgatc ccgcaccggc cgggccggta cgtcatcgtc gatgccggct cggcgcggct
      421 gtggatgatc gacggcgacc gggtcgaggg gccgatgcgg gtcatcgtcg gcaagcccga
      481 catgcagaca ccggccatgg cggggctgat ccgctacgtg accttgaacc cgtggtggaa
      541 tgttccgccc gacctcgcgc gcagccgcgc caggcgggtg ctccggtccg gcccgggctt
      601 cctcgcccgc gagcggatgg aattgctgtc ggattggggc gaccagccca gggcggtcac
      661 ggccgcgcag gtcgactgga aggcggtcgc cggcggtcgg accagcctgc gcatgcgcca
      721 gcggcccggc ggcgacaatg tcatgggcgc gatgaagttc atgcttccca accggctcgg
      781 catctacctg cacgactttc ccgacaagag cctgttcgcc cgcgccgacc gccggctgag
      841 ctcgggctgc gttcggctcg ccgacgctcc gcgtctcgcc cgcttcctgt tcgggggcgc
      901 ggtgccgaga cccgccggcc cggcgccgga gcagcgcgtc gaccttcccg agccggtgcc
      961 cgtctacatc acctacctga cggcgctgcc ggatccggaa aagggcgtgg tgctgcagcg
     1021 cgacgtctat gcgcgcgacg gccgccgatc ctcccggcgc tcggcatgag cggcgggctt
     1081 caccggccgc tccgcttggg ctatcgcgcg gcgatgcgta gatccggacc ggccgtgctg
     1141 cccctcctcc tgctcgccgg ctgcgccggc tatgcggccg attactggaa gcccaagggc
     1201 aatctcatcg ccccccagct gccgcgctac ggcatcagcg ccgaggacag ccgctgcgtc
     1261 ggcgagcggc tgaccaagaa tttgagcgtc tggcagctgc gccagctcgc cgacatcgcc
     1321 cggcgcctcg tcccgcgcga aggcggcgcg ctcgctccgc gcgacctcgc ctacgtggcg
     1381 ggcctcgtcc aggatccgaa ggtcggcccc gaggtcgcgg cgacgctcgc ggcgtgcaac
     1441 gtcggccagg ccgccccggg gcctaccgcc gcctcgacgg cgacccccaa tcccgccgct
     1501 tcccagccag gtctggtgcc gggcgcgact ccgccccagc cgctgcgctg gatcgacctc
     1561 ggcgccgccg agacgggcca gcggatcgcg gtcgacatct cgacggtggt gatgaatgcg
     1621 gagaagcgcg agggctggtt ccgcctctcc aacccaggcc agacgagtcc gagcgccgat
     1681 gcctatcggc tgcgcgtcga ctgcccgggc cggacgatca cgccgacggc ggcgcgcaaa
     1741 tatgattccg acggcaaggt cgtgcagcag gaggattata ccgccgcctg gcagcagccg
     1801 ctgccgatcg aggcggggac ggtgatggag atcgcctgga agcgcctctg cgaatagcgg
     1861 ggccgcggcg ctcgctattc gggcgccgac ctgctagggc cgttccatca cctgatcgtg
     1921 cccgcaagcg tcgagtgaca ggaagacttc gagcttccgc ttgccgcgcg caaaggctgc
     1981 cccatggaac aacgccaccg gaccgaagac gtcggccatt acacccggct cgaggccggt
     2041 gcgcgctggc tcgccgggcg cgccgcagga ggggcggagc gcgacctcca cctcggtcat
     2101 gcccggcgct acgcccggct ccgcgccgac gcgggagggt tttgagatga ccggactgcc
     2161 ccgccggccg accacggtcg agcgcgcctt cgagctggcc cgctcgggcg cctgcgcgaa
     2221 cctgcccgcc atcgccgcgg cgctgaaggg ggagcggcac gacagcgtcg acgcccacct
     2281 cgccggcccg tcgatccgca aggacctgag gcgcgtctgg gaagcggcgg cgcaatagcc
     2341 ggcccgccgg ccgtcgctag cgatggccgt cgagataggt cgaccagccg gtcagcccgt
     2401 cgaacctgag gtcggcatag gtctcgacgt ccttgtcgaa cgagcctcgc gaagcgggga
     2461 accagaaggc gaggcctttg gcccgggcca cggccgcgcc gagcgcgatg gaatggacga
     2521 cggcctcgcg aatcgccccg gcgagaccct cggcggcggc ggcgatctcg gcgatctcgc
     2581 cgtccgcggc gagcttctcg gcgaagtcga gcatgtcgta gctgtcgtag ccgctggcga
     2641 agatctggct ctgcgagcgc gcccagtcca gatagcggaa gcccggccgg cccaggggcg
     2701 aggcgacccg gatcaggtcg gcgaaggcgt cggcgacccc gctgcgggac cggaccgcgc
     2761 tcaacgtcac cggatgctgt tccggccgtc cggcgtagcc cgcttccatc gcctcgaccg
     2821 cctgccgtcc ccaggcctgg ccgtcggcgg ggggcgaggc ggccatgcgc gagaaccatt
     2881 cgtgataatc ccagccgtcg ccgggctcga gctcctcgga gccgacgatg cactgcgcga
     2941 actcctcgaa ttcgctggcc acctcgatca ttccgttcag gcaggtgtcg gagaagatca
     3001 ggtccaccgg ccgctttcgg cccgccgcgg cgaaggagtc gcggagcatg ttgccggcct
     3061 cccggttggt cagcagcccg ccgctctgat cgtcgtggag catcgcccgc agctcgacgt
     3121 cgtgcagcag ctcggcggtg ctgaagaaca ggcgccgggc ggggaaggag cgcgacaggc
     3181 gccagcgcgg caggcgtgcg cgggggagga ccctgcgaag caggccgcgc tcgtcgaaca
     3241 cgccgctgcc atggtcccag aagccgatgg caatgcggga ggtctccgag aagctggcga
     3301 gcgcgcgccc cagcagctcc gagaggacct ccggatcgcc ggtgtcgatc tcgcccagct
     3361 cctcgacgac cgacacgccg tgtcgggccg tgatctcaac gaccctcgcc gggccgttga
     3421 agacgtcggc gacggcgagg acggcgacgt cctcgccgat gccggcggcc atttcggcca
     3481 tgtcctcctc gacgtaatcg gtcagattgt tgtcgccgcc gaacagctcg atgacgacgt
     3541 gggcagcctg cgggtggtgc acggcgaagg gcgaaaccat ggtcgaatcc tccttccgac
     3601 agggcggcgc gaaaggggtt ccgggcggcc gcgagcctcc ccgcgccccc ggcgcctcgt
     3661 ccgcgatatg gaagacggac gcgccggccg cgcgtgacga cggtcacgcg cccggcggcg
     3721 agggtcgccg gctcgacggc cgcgctgggc caggaggggt gggcggggac aggtgccggt
     3781 gccccgccat cggaaggtcg ggggccgctc ggatggcggc tcgggaaggc gctcgcgggg
     3841 atcgacattg cctcgaatcc cgggaggtgc gaagcaggga caagctccga cacccgtccc
     3901 cgagggccgg ccacgaccgg cccgccgagc gggcggcgag gggaaaggcc gggcgatccg
     3961 gcggcgcgcc gagaggcgaa ggagggaacc gagccatgat caccgtcacc gccttcagat
     4021 cggtgccgcc cttcgcgcgc ggcctggtcc gcgacctcag ggtgcgctgg gcgctcgagg
     4081 aggcggggct gcgctacgcg gtgaagctgc tcgccgaggg cgagaaggac ggcgccgaat
     4141 atcgcgcctg gcagccgttc gggcaggtgc ccgcctatga ggagaacggg ctcaaattgt
     4201 tcgaatcggg cgcgatcgtg ctccggatcg ccgagcgcag cgagaccctt ctgccgggcg
     4261 acccggcggc tcgggcgcgg gcggcgcaat ggctgttcgc ggctctgaac acgatcgagc
     4321 cgccgatcct gaacctcgcc gggatcgacc ttttccacgc cgaccaggaa tgggcgcggc
     4381 tgcgccggcc cgacgccgag gcggcggtcc gcaaccggct gggcgagctg gccgcggcgc
     4441 tcggcgacaa gccgttcctc gacgggccgc gcttcaccgc cggcgacctg atgatggcga
     4501 cggtgctgcg catccttcgc cacaccgggc tggtggccga gcagccgcgg ctggccgagt
     4561 ggctcgcccg ttgcgaatcg cggccggcct tcgaacgggc cctggcgggg caattggccg
     4621 atctcgacgc ctgaggcacg gggcccgatc cgtgggtcgg gcagccatca gagcgtcgag
     4681 ggatagttcg aatcgctctc acccgttcgc cccgggcctc tcgaagggct ttgcgtgtcg
     4741 ttccatgcag ggaaaaggag agggcttcga caagctcagc ccgaacggag gtggcggaat
     4801 ttggctgccg agcgctccgg aacggtcctt cgatacgccg cttcgacttc gctcagcggc
     4861 tactcaggat gagcgggggg acgggtaggc ctatcatcga ataggaggcg gacaggaaaa
     4921 taggattgcc acaggccttg gtctgttcta aatatgttct cggcgattcg cgaacgggga
     4981 tgaggaggaa cggacgatgg caggggcccc caaagccttt ctccggccgg cgcgagcgcg
     5041 gctcgcgcct cggtcagccg gccgtgcggg cgaatatccg gtcggtgacc cacgcggtcc
     5101 gggcggcgct ttcgccggtc gaggggtggg gcgcctcgcc ggacagatgg cggatcatgt
     5161 tctcgacgtg atggagctgg atcgggtccg gattggggat ctcgaccgtc cggccgcccg
     5221 cgcccgtctc caggaccagc ggcgcctcct cgaagacgga gaaggagatc cgcccctcgg
     5281 agccgaggag gcggacctcg tcctcgacac ggcaggcggc gaaggtccag aagcccgacc
     5341 cggtcgcccc gcccgaatgg ctccacgccg cggcgacgga atcggggacc gcgtacagtc
     5401 cggcccggtt gcggtggatg ccggcggcct cgtcgatcgg tccgagcagg aaatcgaaca
     5461 ggtcgagccc gtggcaggcg agatcctcga aatagccgcc cggcgcctcg gcgggatcgg
     5521 cgcgccagcc gggatcgccg gagaggtcgc ccggcgtcgg ggcgcgcgcc agggcccagt
     5581 ggacgtggcg gacctcgccg atcgcgccct ccgcgatcca gcgccgcacc tgggcgaagc
     5641 ggggaaggga gcggcggtaa taggcgacga acagaggctg gccggccgcc cggaaggcat
     5701 cgaccatcgc ggccgcctcg gcgtgggaca aggcgatcgg cttctcgacg cagcagggct
     5761 tgccggccgc ggcgaccttc gccgccagcg cccggtgcga ggagggcggg gtggcgatgt
     5821 agacggcgtc gacctccggc gagcggatga gggcgtcggg atcgtcgaag acgaggccga
     5881 tcccgcgcct ccccgcatag gcccgggcgg cttccggccg gcgcgaggcg accgcgacca
     5941 gccgcgaccc ggcggcctgc gcataagcgg gcgcgctctt cctctcggtg acggcgccgc
     6001 agccgatcat gccccatcgc accatgtcca agcgccttcc tcccgcccga cgagcccggc
     6061 tccgagcgaa ttgccgagcg tgccgactag ggtgaatgac ggccgggggc tacgccaaat
     6121 cgcccgcgcc agcgattccc ggacggcctt tcccgccgac gggcgcgaga aagccggcgg
     6181 cgcgcgacag ttacgccggg gaaacgacag gaactcgcgc cgcgaagcgg ggtcgagccg
     6241 ccggtcacgc agagatgcga ataaaataga tcaattccgc ggtgctcgat gtgctgcgtc
     6301 cgtacgcagt tctacttacg cgagcgactt aagtcacgga agctattgca gaattagatt
     6361 tcgtatagta gtcgccgccg ccgccaggga ggccgcctgt ggggggcgcc gtctccatcc
     6421 tcaaatcccg aacctagtga gcgtgaagtg accgccttgt cttcgtttac ccgattcgaa
     6481 ctggccgcgt tgagggcctt caccgacgcg cttcttccgg gcgaagcgca gtctcccggc
     6541 gcggccgagc tcgaagtggc cgagttcctg gacgacttcg tcggcgcgtc gccggccggc
     6601 gaggccgagc tctggcgctc gggcctgaag gcgctggacg accgggcacg ctcggacttc
     6661 ggccgggcct tcgccgacct cggcgaggcc gagctcggcg aattgctgac ggccctgctc
     6721 gatccggccg ccgcgccggc gccgctcgcc gatttcgccc gccgggcgaa ggccgccgcg
     6781 atccgcgcct attaccgcac cgagcacggc cttcacggcg agctcaaggt ggcgaggccc
     6841 gcgcaggcgg cgccgtcggc cgcgttcgag ggcgggtttc ccgagccgga ccggtcacgg
     6901 acccgctacg acgtggtcat cgtcgggtcc ggcgcgaccg gcggctgggc ggcgaaggag
     6961 ctgaccgagg ccggcttcga ggtgctggtg ctcgattcgg gcggaatgcc gccgccggcc
     7021 gggcaggtgc ggcagcccta ccaggtccgc ttccgcaaca tgttcgacaa catgaccctg
     7081 aacggggcgc gccagccggt gcaggcgcgg tcctgggcct gcgacgagta ccgctccgcc
     7141 ctgttcatcg acgaccgcga gcatccctat gagggcgccg acggcttcac ctggatccgc
     7201 gcctcctcgg tcggcgggcg gatgaacatg tggggccggc aggtctaccg ctattcgagg
     7261 caggacctgg cggcgtccaa catggagggc cggtcagagc cctggccgct cgactatgac
     7321 gagctcgcgc ccttctacga cgaggtggag cggttcatcg gagtcagcgg cgcgctcgac
     7381 ggctgcgagc atctgcccga cggcaagttc ctgccgccga tggcgatgac cgacggcgag
     7441 aagcatctga agcaggcggt ggagagccgc tggcccgacc ggcgggtgat catcggccgg
     7501 accgcgaccc tcacccgcga ccatgagggg cgcaaggcct gcgacggcgc cggccattgc
     7561 taccgcggct gcccgacgcg ctccttcttc gagccgctgg gatcgaccct ggcctcggcg
     7621 cgcgcgaccg gccggctgca cctgatgcca aaggcgcatg tgctgcggct cgtcatggac
     7681 gaggagaagg gcgaggcgcg cggggtggcc tatgtcgacc tgcgcgacgg cagcgagcat
     7741 ctggtcgagg cccgggccgt cgttctcgcc gcctcgacga tcgcctcgac ccggatcctg
     7801 atgtcgtcgc ggactccgcg gcttccggac ggcgtcggat cgtcgtcggg cgtgctcggc
     7861 gcctatctcc acggccatat ccactcggtg atgtgctccg ggcaggtgcc gtggctgctc
     7921 aagccggcgg ggatgcacga cgagggccgg cccaaccaga tctacatccc gcaattcctg
     7981 aacaatgcgg ggcagaagcc ggagccgggc ttcgtcggcg gcttcggggt cgagggcgcg
     8041 gtcaagcact acatgttccc gagggacatg gcggtacgcc cgggcttcgg ccgcgacctc
     8101 aagcggagca tccgcgagga ccgggtgccg gcgcatttct tcctcaccgc cttcggagtc
     8161 atgctggcgc ggccggagaa ccgggtggag ctcagcggct ccaccgacaa atggggcgtc
     8221 ccgacgatca aggtgagctg cggttacggc gacaacgacc tgcggatggc ggaggcgatg
     8281 gcgaagagcg tcgtcgagat cgccgaggcg gccgacttca ccgtctcttc ggtgtcgccc
     8341 gcgcccggat tgcccggcat gtgcgtccat gaggtcggca cggcgcggat gggaagcgac
     8401 gcctccagct cggtgctcga tccgtggaac cgggtgtggg actcgcccaa cgtgctcgtc
     8461 gtcgacggcg cctgcttccc gtccagcggt ccgcagaatc cgaccctgac catgatggcg
     8521 ctg
//
LOCUS       DASVQZ010000139         7135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_799514, whole genome shotgun sequence.
ACCESSION   DASVQZ010000139 DASVQZ010000000
VERSION     DASVQZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799514"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VF547_03910"
     CDS             complement(<1..367)
                     /locus_tag="VF547_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX8262001.1"
                     /translation="MPGRALRRLVERWRLGALLLAAGAGLATLATGSGDGIDQALTDA
                     RAAWSERSASGEIHIVEIDDRSIAAFRQWPWPRHVHGAAVDRLRESGAALIAFDVDFS
                     SPSRPGEDAAFAAALRRAGG"
     gene            complement(369..1502)
                     /locus_tag="VF547_03915"
     CDS             complement(369..1502)
                     /locus_tag="VF547_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HEX8262002.1"
                     /translation="MKGRRVMLASLCLASIGLAGAAPPRLEVAAWEPDGAVLLAGAES
                     ASADVPVEPGEQYPPTAREEATAPAAAPARTGPPPARRRTDWRLSVDMSVTVDSNVTN
                     GTKLHAVPIDLGEGPLPVPVDPNLRERADVGAGVSAAANVRVPVAAEAALAVDVEGFA
                     VEYGGRRSDDASLLVAAGLEFGAGPAPEASLQLIAFDRRYGGVKALEGIGMRGHWRHG
                     LAARESLRLAVDARIFSSGYGDRFGGHEASLYVSYDTVLAPTLSASAGVYAHRQWLGD
                     DAFSSLDFGAYGGLSAYLGDDLAGGISAGVSRTLFDEPFLLLGPEARGDWRGYASAWL
                     TTRRPVALGLHPSVTYTYNRTSSSIGYYDSDRHRLRLGLKRQF"
     gene            complement(1589..3019)
                     /locus_tag="VF547_03920"
     CDS             complement(1589..3019)
                     /locus_tag="VF547_03920"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX8262003.1"
                     /translation="MILRALIVMLLCWSHAAFAETPLWRVTEVAGPVQLQHGPRTAAA
                     VRGATLAPGDTVTTGVNGRAVLVRGQEYVIVAARSRLRLPAAAEARGIVQMLQDFGRA
                     TFRIEKKATPHFGVRTPYLVALVKGTTFTVSADTAGATVAVEEGRVEVATPDGGARQM
                     VDAGMAATLSSARPAEIAVRPAAEVAAAQPDEAGPGCADEGARGCGPRLAYERVDPVE
                     TVSAPAALPGGAGPAEVPLAAAVIELAAAPAAPAPAPAPAPAPAPAPGAGPLPDPGAT
                     PAVPATPAPGGPAAPAVPAVPAVPATPTVPAVPAAPAVPPPGTAPVPAPAPAPTPPPP
                     ADPGSNSGPGSNSGPGSNSGPGSNSGPGSGTPQPPAPDPAPAPAPAPAPAPEPAPAPA
                     PAPAPEPVPAPAPAPAPAPAPAPEPAPAPAPAPPPAPAPDPAPAPEPVPTPPPPELVP
                     APMLPPPPPPPSTPTCKLINLLMVCI"
     gene            complement(3116..3823)
                     /locus_tag="VF547_03925"
     CDS             complement(3116..3823)
                     /locus_tag="VF547_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8262004.1"
                     /translation="MRTAAAWAVHLFTASGVVLAFLALTAIDQGDWRLALLWLLAALA
                     IDGVDGTLARLARVKEHAPRIDGEAFDLIVDYLNYVFVPAVFICRAGLVPAALTLPLA
                     AAILLSSVYVFVRRDMKTHDNYFRGFPALWNVVALYLFAARPGEAAGAVAVAVFAVLS
                     FAPIRFVHPFRVRDFQPWLQIVALLWAAATLALLWPGWSAAAGRAWLLASLALAAVLL
                     AVGLLRSLRGPSSPPPG"
     gene            complement(3939..4160)
                     /locus_tag="VF547_03930"
     CDS             complement(3939..4160)
                     /locus_tag="VF547_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX8262005.1"
                     /translation="MSKLHLVFGGRVKDPRGLDFDLDTIDLVGLFPDYASALDAWRGR
                     AQRTVDDAEMKYVVVHLHRLLEPELGKAE"
     gene            complement(4182..4688)
                     /locus_tag="VF547_03935"
     CDS             complement(4182..4688)
                     /locus_tag="VF547_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262006.1"
                     /translation="MSSTTDDRPAAASATNRGAAAEERESRIPAARQAYESARERTAA
                     AYAAARERAGSAYETAGRRAGEGIESNPLAAVFGGLALGAIVAAMLPRTRREEELLGD
                     VGRRLSDSAREAARAAKEAGRGQLDELGLSRDGLKNKLDEFTGRAVGAIKDSAGAAAG
                     AARGKGKA"
     gene            complement(4685..5029)
                     /locus_tag="VF547_03940"
     CDS             complement(4685..5029)
                     /locus_tag="VF547_03940"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX8262007.1"
                     /translation="MSADDLRLAKQEAERARRRLAATAAELQERLKPGTLASQAWAGV
                     KDKGGDLADDAVEAVKARPVVVSAALAAFTLFLARAPIRSAVGRLIEGERDEEDAKDA
                     RPRRARREGASV"
     gene            complement(5026..5409)
                     /locus_tag="VF547_03945"
     CDS             complement(5026..5409)
                     /locus_tag="VF547_03945"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8262008.1"
                     /translation="MDAPASREEESSLGELFGRLSEDARAYASAEAKLYQAIARRRIG
                     RARNGAIALVAAALLANAALLVLLIGLSLELARLVGPALAGLIVTVAVLGIGFLLARY
                     GAAKLGALSGDPEESAALQAGERLT"
     gene            complement(5412..6071)
                     /locus_tag="VF547_03950"
     CDS             complement(5412..6071)
                     /locus_tag="VF547_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262009.1"
                     /translation="MPPRDDELPEGTDHIINGAMETGGGDSGTAGGGGASSGFIGASG
                     GDGTGGTAAGGGGIDMTASDGPDQAGDTAGEGNEGGGVADQVRGQIYNLRDQAGGKVR
                     EFADTGKTRATDLLEELSRVVADTADSIDERLGNNYGEYARKAADSVSGLAENLRGKD
                     VDELYENVRSAVRKSPGVAIGIAAVVGFTLVRLVKAGLPDEDEGARGGRRRTNSGGSG
                     A"
     gene            6231..>7135
                     /gene="eno"
                     /locus_tag="VF547_03955"
     CDS             6231..>7135
                     /gene="eno"
                     /locus_tag="VF547_03955"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX8262010.1"
                     /translation="MTAILDVHARQILDSRGNPTVEVDVLLEDGSMGRAAVPSGASTG
                     AHEAVEKRDGDKSKYLGKGVCEAVNSVRGEIFVAIAGRDAEDQGEIDALLIDLDGTEN
                     KSRLGANAILGVSLAVAKAAADARGLPLYRYVGGVSAQLLPVPMMNILNGGAHADNPI
                     DFQEFMVMPVGAGSLAEAVRCGSEIFHTLKKNLHDAGLSTAVGDEGGFAPNLASARDA
                     LDFIMKSVEQAGYEPGDDVVLALDCAATEFFSDGAYRMEGEGKTLAPAEMADYLAGLA
                     RAYPIASIEDGMAEDDLEGWKALTDA"
BASE COUNT          997 a         2503 c         2705 g          930 t
ORIGIN      
        1 tccccccggc gcggcgcaga gcggcggcga aggcggcgtc ctcgcccggc cgcgacgggc
       61 tcgagaagtc gacgtcgaac gcgatcagcg ccgcgccgct ctcgcgcagc cggtcgacgg
      121 cggcgccgtg gacgtggcgg ggccagggcc attgccggaa ggcggcgatg cttcggtcgt
      181 cgatctcgac gatgtggatc tcgccgctgg cggaacgctc gctccaggcg gcgcgcgcgt
      241 cggtcagcgc ctgatcgatg ccgtcgcccg acccggtggc gagcgttgcg agcccggcgc
      301 cggcggcgag cagaagcgcc cccaggcgcc accgctcgac aagtcggcga agcgctcggc
      361 cgggcatgtc agaactgccg cttcaggccg agccgcaggc gatgccggtc ggaatcgtaa
      421 tagccgatcg agctcgacgt gcggttgtag gtgtaggtga cggaagggtg gaggccgagg
      481 gccaccgggc gccgggtcgt cagccaggcg ctggcatagc cccgccagtc gccccgcgcc
      541 tcgggcccga gcaggaggaa gggctcgtcg aacagcgtcc ggctgacgcc ggcgctgatc
      601 ccgccggcga ggtcgtcgcc gagataggcg ctgaggccgc cataggcgcc gaagtcgagg
      661 ctcgagaagg cgtcgtcgcc gagccactgg cgatgggcgt agacgccggc agaagcggac
      721 agcgtcgggg ccaggaccgt gtcgtagctg acgtagaggc tggcctcgtg gcctccgaag
      781 cggtcgccat atcccgagct gaagatgcgg gcgtcgacgg ccaggcgcag gctttcgcgc
      841 gccgccagcc cgtggcgcca gtggccgcgc atcccgatgc cttcgagggc cttcacgccg
      901 ccgtaccggc ggtcgaaggc gatcagctgc agcgaggcct cgggcgccgg cccggccccg
      961 aattcgaggc cggccgcgac cagcagggag gcgtcgtcgc tgcgccgacc gccatattcg
     1021 acggcgaagc cctcgacgtc cacggccaag gcggcttcgg ccgcaaccgg gacccggacg
     1081 ttggcggccg ccgagactcc ggctccgacg tcggcgcgct cgcgaagatt gggatcgacc
     1141 ggcaccggga gcggaccctc tccgagatcg atcggaacgg cgtgcagctt ggtgccgttg
     1201 gtgacattgc tgtcgacggt gacgctcatg tcgaccgaaa gacgccagtc ggtgcggcgg
     1261 cgggccggcg ggggcccggt cctggcaggg gccgcggcgg gcgccgtcgc ttcctcgcgc
     1321 gcggtggggg gatattgctc accgggctcg accggcacgt ccgcggaggc ggattcggcc
     1381 ccggccagca agacggcccc gtccggctcc cacgcggcca cctcgaggcg cgggggagcg
     1441 gctccggcca ggccgatcga ggccaggcac agcgacgcca gcatcacccg ccgtcccttc
     1501 atgcgccgtc ctgcctccgc catccgcccc gtgaggcgac ggtcttagcg gaacgaggtt
     1561 gaggaaccgt tagggatgag gcggaccttc agatgcagac catcagcagg ttgatcagct
     1621 tgcacgtcgg cgtggacggc ggcggcggcg gcggcggaag catcggtgcc ggcacgagct
     1681 caggcggcgg cggcgtcggg accggctcgg gcgcgggtgc ggggtccgga gccggcgccg
     1741 gcggcggagc cggagccgga gctggtgccg gctcaggagc gggggcaggc gccggagcgg
     1801 gcgcgggagc cggcgccggg acaggctccg gagcgggcgc gggagccggc gcaggcgcag
     1861 gttccggagc gggcgcgggg gccggcgcag gcgcgggcgc ggggtcggga gcgggcggtt
     1921 gaggcgtccc gctgccgggc ccggaattgg agccgggacc ggaattcgag ccgggaccgg
     1981 aattcgagcc tggacccgag ttcgagccgg gatcggccgg cggcggcggc gttggggcag
     2041 gagcgggagc cgggaccgga gcggtgccgg gcggcgggac ggcaggagca gccggaaccg
     2101 ccgggactgt gggggttgcc gggaccgccg ggaccgccgg gaccgccgga gcggcagggc
     2161 cgccgggcgc cggtgtggcc ggcacggccg gggtcgcccc gggatcggga agcggccccg
     2221 ctccgggtgc cggagcaggc gcgggcgcag gcgcaggcgc tggagctggc gccgcagggg
     2281 ccgcggcgag ctcgatcacc gccgccgcca gaggtacctc ggccggaccc gcgccgccgg
     2341 gcagcgccgc cggagccgat accgtctcga ccgggtcgac ccgttcgtaa gcgagcctgg
     2401 ggccgcagcc gcgcgcgccc tcgtcggcgc agcctggccc ggcctcgtcc ggttgggccg
     2461 cggcgacctc ggccgccggc ctgaccgcga tctcggccgg acgggcggag ctcagggtcg
     2521 ccgccatgcc ggcgtcgacc atttgccggg cgccgccgtc aggggtcgcg acctcgaccc
     2581 gcccttcctc gaccgccacc gtcgctccgg ccgtgtcggc gctgacggtg aaggtcgtgc
     2641 ccttgacgag cgcgacgaga tagggcgtcc gaactccgaa atggggcgtc gccttcttct
     2701 cgatgcggaa ggtggcgcgt ccgaaatcct ggagcatctg cacgattccg cgcgcctcgg
     2761 cggccgccgg caggcgcagg cgcgaccgcg cggcgacgat cacatattcc tgtcctcgca
     2821 ccagcaccgc ccgcccattc accccggtcg tcaccgtgtc gccgggggcg agcgtggcgc
     2881 ctcggaccgc ggccgccgtc ctcggcccgt gctgaagctg aaccggcccg gccacttcgg
     2941 tcacccgcca cagcggggtt tcggcgaagg ccgcgtggct ccagcacagg agcatgacga
     3001 tgagggcgcg caggatcatg gtcggtcact ttccgcagca gccgctatgg cccggggggc
     3061 tcccgcttaa aggcgaaagc ttgcgagggt ctgaggagcc gtggctaaag cgccgttaac
     3121 cggggggagg ggaggacggc ccgcgcaggc tgcgcaggag gccgacggcg agcaggaccg
     3181 cggccagggc cagcgaggcg agcagccagg cccggcccgc cgccgcgctc cagccgggcc
     3241 acagcagagc gagggtggcg gccgcccaga gcagggcgac gatctgcagc cacggctgga
     3301 aatcccggac ccgaaacggg tggacgaaac ggatgggcgc gaagctcagc acggcgaaga
     3361 ccgcgaccgc gaccgctccg gccgcctcgc cgggccgcgc ggcgaacagg tagagcgcga
     3421 cgacgttcca cagcgccggg aagccgcgga aataattgtc gtgagtcttc atgtccctgc
     3481 ggacgaacac atagaccgag gaaaggagga tcgcggccgc cagcggcagc gtcagggccg
     3541 ccggcaccag gccggcccgg cagatgaaga cggccgggac gaacacgtag ttcagataat
     3601 cgacgatgag gtcgaaggcc tcgccgtcga tccgcggcgc atgctccttc acccgggcga
     3661 gccttgccag cgttccgtcc accccgtcga tggccaaggc ggcgagcagc cagagaaggg
     3721 cgagccgcca gtcgccctgg tcgatcgcgg tcagggccag gaacgcgagg acgactccgc
     3781 tggccgtgaa caggtggacc gcccaggcgg cggcggtgcg catcagcggc gctcccttgg
     3841 cgggctcgga gggccgccga gtcccgatcg gccggagcgc gtgccccgcc gacccgctcc
     3901 ggacggtctc cctccccatc ccgcgatggg gaggcggatc attccgcctt ccccagctcc
     3961 ggctcgagca ggcggtggag gtggacgacg acatatttca tctcggcgtc gtcgaccgtg
     4021 cgctgggccc gcccccgcca ggcgtcgagc gcgctggcat aatcgggaaa caggccgacc
     4081 aggtcgatgg tgtcgaggtc gaaatccagg ccgcgcgggt ccttgacccg gccgccgaag
     4141 acgaggtgaa gcttgctcat gggcgcgccg gcaaggaggc tttacgcctt gcccttgccg
     4201 cgtgcggcgc cggcggccgc gcccgcggaa tccttgatcg ccccgacggc gcggccggtg
     4261 aactcgtcga gcttgttctt cagcccgtcg cggctgaggc cgagctcgtc gagctgaccg
     4321 cggccggcct ccttggcggc gcgggccgcc tcgcgggcgc tatcgctgag acggcggccg
     4381 acatcgccca gaagctcctc ctcgcggcgg gtgcgcggca gcatcgcggc gacgatcgcg
     4441 ccgagggcga ggcctccgaa cacggcggcg agcggattgg actcgatgcc ctcgccagcg
     4501 cggcggccgg cggtctcgta ggcgcttccc gcgcgctccc gggcggccgc ataggccgcg
     4561 gcggttcgct cgcgggcgct ttcataggcc tggcgcgcgg cggggatgcg gctttcccgc
     4621 tcctcggcgg ccgcgccgcg attcgtggcc gaggccgcgg cgggccgatc gtcggtagtg
     4681 ctgctcatac gcttgctcct tctctacggg cccgccgcgg gcgggcatct ttggcgtcct
     4741 cttcgtcgcg ttcgccttcg atgaggcggc cgaccgccga ccggatcggc gcccgcgcga
     4801 ggaagagggt gaaagcggcc agggcggccg agacgacgac gggccgggcc ttgaccgcct
     4861 cgacggcgtc gtcggcgagg tcgccgccct tgtccttgac tccggcccag gcctggctgg
     4921 cgagggtgcc gggcttgagc ctctcctgaa gctcggcggc cgtcgccgcg agccggcggc
     4981 gggcacgctc ggcctcctgc ttggcgagcc gcagatcgtc ggcgctcacg tcagcctctc
     5041 cccggcctgg agcgccgcgc tctcctcggg atcgccggac agcgcgccga gcttggcggc
     5101 gccgtagcgc gccagcagga agccgatgcc gagcaccgcg accgtcacga tcaggccggc
     5161 cagggcgggt ccgacgagcc gcgcgagctc gagcgacagt ccgatcagca gcaccagcag
     5221 ggccgcgttg gcgagcaggg cggccgccac cagagcgatg gcgccgttgc gggcgcgccc
     5281 gatgcgccgc cgggcgatgg cctggtagag cttcgcctcg gccgacgcat aagcgcgcgc
     5341 gtcctcggaa agccgtccga acagctcgcc gagcgagctt tcctcttccc tgctcgccgg
     5401 cgcgtccaag ctcaggctcc cgagccgccg ctgttggtcc ggcggcggcc gccgcgggct
     5461 ccctcgtcct catcgggcag gccggccttg accagccgaa ccagcgtgaa gccgaccacg
     5521 gcggcgattc cgatggccac tcccggactc ttgcggaccg ccgagcggac gttctcgtag
     5581 agctcgtcga cgtccttgcc gcggaggttc tcggcgaggc ccgacacgct gtccgccgcc
     5641 ttgcgggcat attcgccata attgttgccg agacgctcgt cgatcgagtc ggcggtgtcg
     5701 gcgacgaccc gggaaagctc ctcgagcagg tcggtggcgc gagtcttgcc ggtgtcggcg
     5761 aactcgcgga ccttgcctcc ggcctggtcg cgaagattgt agatctggcc gcgcacctgg
     5821 tcggcgactc cgccgccctc atttccttcg ccggcggtgt cgccggcctg gtccggacca
     5881 tcgcttgccg tcatgtcgat tcctcctcct cccgcggccg ttccgccggt gccgtcgccg
     5941 ccggacgcac cgatgaagcc ggagctggcc ccgccgccgc cggccgttcc gctatcgccg
     6001 ccgccggttt ccatggcgcc gttgatgatg tggtcggtgc cctcgggcag ctcgtcgtcg
     6061 cggggaggca taggctttct ccaactctga agtttactcg catcgcaaac cggcgaggcg
     6121 gggctcgcgt tcccggcccg gcgacgaaag ttgcgtcgcc cgagccgcgc cgataagccg
     6181 cgccgacgcg cattgccttc acccatatag ttcagaacga ggccgtaaca atgaccgcca
     6241 tcctcgacgt ccacgcccgc cagattctcg acagccgcgg caacccgacg gtcgaggtgg
     6301 acgtgcttct cgaggacgga agcatgggcc gggcggcggt gccgtcgggc gcctcgaccg
     6361 gagcgcacga agcggtcgag aagcgggacg gagacaagag caaatatctc ggcaaggggg
     6421 tgtgcgaggc ggtcaattcg gttcggggcg agattttcgt ggcgatcgcc ggacgcgacg
     6481 cggaggacca gggggagatc gacgcgcttc tgatcgacct cgacgggacg gagaacaagt
     6541 cgcgcctcgg ggccaacgcc atactgggag tcagcctcgc ggtcgccaag gccgccgccg
     6601 acgcccgcgg cctgcccctc taccgctatg tcggcggggt ctcggcgcag ctgctgccgg
     6661 tgccgatgat gaacatcctg aacggcgggg cccacgccga caatccgatc gacttccagg
     6721 agttcatggt gatgccggtc ggcgccggct cgctcgccga ggcggtgcgc tgcggctcgg
     6781 agatcttcca tacgctgaag aagaacctgc acgacgccgg tctgtccacc gcggtcggcg
     6841 acgaaggcgg cttcgccccg aacctcgctt cggcccgcga cgcgctcgac ttcatcatga
     6901 agtcggtcga gcaggccggc tacgagccgg gtgacgacgt cgtcctggcg ctcgattgcg
     6961 cggccacgga gttcttcagc gacggcgcct accgcatgga aggggagggg aagaccctcg
     7021 cgcccgccga gatggccgat tatctggccg ggctggcgcg cgcctatccg atcgcctcga
     7081 tcgaggacgg catggccgag gacgatctcg agggctggaa ggcgctgacc gacgc
//
LOCUS       DASVQZ010000140         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_318557, whole genome shotgun sequence.
ACCESSION   DASVQZ010000140 DASVQZ010000000
VERSION     DASVQZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318557"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VF547_03960"
     CDS             <1..565
                     /locus_tag="VF547_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8262011.1"
                     /translation="SGTEIATISDISMIKLDFPVPETVLATLKPGLTIEARSAAWPDQ
                     PFRGQIANIDPVIDPNTRAVMVRAHLPNSDGKLKPGMLLTVAIETSPRLGLSVPELAV
                     IGEGDSRFVYTVDGSGTARRTAVRTGLRTAGRVEILEGLKPGQKVVTEGVVKLTDGMK
                     VRLAGARNSEAGPRTKGPGAQAQGRAP"
     gene            678..3797
                     /locus_tag="VF547_03965"
     CDS             678..3797
                     /locus_tag="VF547_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8262012.1"
                     /translation="MKLSDISVRRPVFAAVLAMLIAVIGLVGFFGLSVREYPDVDPPV
                     VSVQTQYIGAAASVVETRVTQVLEDQLSGVEGLQTITSRSQDGQSSITIEFYPGRSIE
                     SAANDVRDRVGAAAGNLPDDVRPPEVRKVDADASPILFLVVSKPGWSRLELSDYVDRN
                     LIDRFSSIEGVARVFVGGEARPAMRVWMQPGRLAAFGLTPADLEGALRRQNVELPAGR
                     LESAQQNVTLRVDRPFANAQQFSQLVVGRGADGYLVRLGDVARVEQGPENPYSAFRLN
                     GEPAVGLGIVRQSGANTLAVAEAAKRTAEELKPSLPEGMSLVVGSDDSLFISRAIEKV
                     WETLAEAAVLVVLVIYLFLGSWRATLIPAVTVPLCLLASFAVLWLFGFSINLLTLLAM
                     VLSIGIVVDDAIVVLENVYHRIEEGEPPLAAAFEGTRQVAFAIISTTLVVCAVFVPIM
                     FIAGQTGLLFRELAAAMIGAVAFSGFLALSLAPMLCSKLLRYEERGRLARFIDDRFRR
                     FEAFYSRRLDTVIRRPVVPLAIVGLFLAVSAALFMTLKSELVPAEDVGILSANVNAPE
                     GTGYDQMDRYMVDAQGKLLPLVENGAVRAIITRTPGGFGQSDDFNSGTFVLFLKPWEE
                     REKTTQDVVQEVNRRLSQMPAVRGNAQVRSSIGGRGRGNPISFVIAGATYADLARARD
                     RILAAAAANPGIVNLDSDYKETKPQLRIAVDTARAGDLGVSVEEVSNALQSLLGSRRV
                     STYVDRGEEYRVIVQAEAGSRATEANLQTIHVRSRSGGVVPLASLVTVNDQAGARDLG
                     RYNKMRAITLSGGLAPGYSLGEALTFLEAQAAASPEVVAVGYRGESQAFREAGSSILL
                     VFLLTILIIYLLLAAQFESFVHPAVIITTVPLAVGGGIVGLAVMGQTLNLYSQVGIVM
                     LVGLAAKNGILIVEFANQLRDAGRDIGEAIREASARRLRPILMTSIATAAGAVPLMIA
                     SGAGAGARQAIGVVVVFGVSIATVITLFLIPVLYSRLARYTGSPLAVTRKLEEQLRRP
                     GHAHPAPAE"
BASE COUNT          557 a         1385 c         1262 g          616 t
ORIGIN      
        1 cagcggcacc gagattgcga cgatcagcga catctcgatg atcaagctcg atttcccggt
       61 gccggagacg gtgcttgcga ccctgaagcc gggcctgacg atcgaggccc ggtccgccgc
      121 ctggcccgac cagccgttcc gcggccagat cgccaatatc gatccggtga tcgaccccaa
      181 cacgcgagcg gtcatggtcc gggcgcacct gcccaatagc gacggcaagc tgaagccggg
      241 catgctcctg acggtggcga tcgagacctc gccgcgactc ggcctgtcgg tgccggagct
      301 ggccgtgatc ggcgagggcg acagccgctt cgtctatacg gtcgacggga gcggcaccgc
      361 gaggcgcacc gccgtccgga cggggcttcg aaccgccggc cgggtcgaga ttctcgaggg
      421 cctcaagccc ggccagaagg tggtgaccga gggcgtcgtc aagctcaccg acggaatgaa
      481 ggtgcggctg gccggcgctc gaaactccga agccgggccg cggacgaagg gcccgggggc
      541 gcaggcgcag ggcagggcgc cgtgagcttc tccccgtcat tgcgagcgca gcgaagcaat
      601 ccagcgctga gtccgtcgca ttcgccgcag ctggattgct tcgtcgctcc gcgcctcgca
      661 atgacggcgg aaggcgcatg aagctttccg acatctcggt ccggcggccg gtcttcgccg
      721 ccgtgctggc gatgctgatc gcggtgatcg gcctggtcgg cttcttcggc ctgtcggtgc
      781 gcgaatatcc ggacgtcgat ccgcccgtcg tctccgtcca gacccagtat atcggcgccg
      841 ccgcgagcgt ggtcgagacc cgggtcaccc aggtgctcga ggatcagctc tcgggcgtcg
      901 agggccttca gacgatcacc tcgcggagcc aggacggcca gtcttccatc accatcgaat
      961 tctatcccgg ccgatcgatc gagtcggcag ccaacgacgt ccgcgaccgg gtcggagcgg
     1021 cggcgggcaa tctcccggac gacgtccgcc cgccggaggt gcgcaaggtc gacgccgacg
     1081 cctcgccgat cctgttcctg gtcgtctcca agcccggctg gtcgcggctc gagctgagcg
     1141 actatgtcga ccgcaacctg atcgaccgct tctcgtcgat cgaaggcgtc gcccgggtct
     1201 tcgtcggcgg cgaggcgcgc ccggcgatgc gcgtctggat gcagcccggt cggctcgccg
     1261 ccttcggcct cacccccgcc gatctcgaag gcgctctgcg ccgccagaat gtcgagctgc
     1321 ccgcgggccg cctcgaatcg gctcagcaga acgtcaccct ccgggtcgac cggccgttcg
     1381 ccaacgctca gcaattctcc caattggtcg tcggccgcgg cgcggacggc tatctggtcc
     1441 gcctcggcga cgtcgcccgg gtcgagcagg gaccggaaaa tccctacagc gccttccggc
     1501 tcaacggcga gccggcggtc ggcctcggca tcgtccgcca gtccggcgcc aacacgcttg
     1561 cggtcgcgga ggcggccaag cggacggccg aggagctcaa gcccagcctg ccggaaggca
     1621 tgagcctggt cgtcggctcc gacgattcgc tgttcatcag ccgcgccatc gagaaggtgt
     1681 gggagaccct ggccgaggcc gcggtgctgg tggtgctggt catctacctg ttcctcggca
     1741 gctggcgcgc cacgctcatc cccgccgtga cggtgccgct ctgcctgctc gccagcttcg
     1801 ccgtgctctg gctgttcggc ttctcgatca acctgctcac cctgctggcg atggtcctgt
     1861 cgatcggcat cgtcgtcgac gacgcgatcg tcgtgctcga gaacgtctac caccggatcg
     1921 aggagggcga gccgccgctc gccgccgcct tcgaaggcac gcgccaggtc gccttcgcca
     1981 tcatctcgac cacgctcgtc gtgtgcgccg tgttcgtgcc gatcatgttc atcgccggcc
     2041 agaccggact gctgttccga gagctcgccg cggcgatgat cggcgccgtc gccttctcgg
     2101 gcttcctcgc gctcagcctc gcgccgatgc tctgctcgaa gctgctgcgc tacgaggagc
     2161 gcggccgact tgcccgcttc atcgacgacc gcttccgccg cttcgaggcc ttctactcgc
     2221 gccggctcga caccgtgatc cgccggcccg tggtgccgct cgcgatcgtc ggcctgttcc
     2281 tggccgtgtc ggcggcgctg ttcatgaccc tcaagtcgga gctggtcccg gccgaggacg
     2341 tcggcatcct ctcggcgaac gtcaacgccc ccgaaggcac cggctacgac cagatggacc
     2401 gctacatggt cgacgctcag ggcaagctgc tgccgctcgt cgaaaacggc gcggtccggg
     2461 cgatcatcac ccgaacgccc ggcggcttcg gccagagcga tgatttcaac agcggcacct
     2521 tcgtgctctt cctcaagccc tgggaggagc gcgagaagac cacccaggac gtcgtccagg
     2581 aggtgaaccg gcggctgagc cagatgccgg cggtccgcgg caacgcccag gtgcgctcgt
     2641 cgatcggcgg ccgcggccgc ggcaacccga tcagcttcgt catcgccggc gccacctatg
     2701 ccgacctcgc ccgggcccgc gaccggatcc tcgccgccgc ggccgccaat cccggcatcg
     2761 tcaacctcga ttccgattac aaggaaacca agccgcagct gcgcatcgcc gtcgacaccg
     2821 cgcgtgccgg cgatctcgga gtctcggtgg aggaggtctc caacgcgctt cagagcctgc
     2881 tcggatcgcg ccgggtctcg acctatgtcg accgcggcga ggaatatcgc gtgatcgtcc
     2941 aggccgaggc cggctccagg gcgaccgagg cgaacctcca gaccatccac gtgcggagcc
     3001 gctcgggcgg agtggtgccg ctcgccagcc tggtcaccgt caacgaccag gccggcgcgc
     3061 gcgacctcgg ccgctacaac aagatgcgcg cgatcaccct ctccggcgga ctcgccccgg
     3121 gctactcgct gggcgaggcc ctgaccttcc tcgaggccca ggcggccgcc tcgccggagg
     3181 tcgtcgccgt cggctatcgg ggcgagagcc aggccttccg cgaggcggga agctcgatcc
     3241 tgctcgtctt cctgctcacc atcctcatca tctacctgct gctcgccgcg cagttcgaaa
     3301 gcttcgtcca cccggcggtg atcatcacca ccgtgccgct ggcggtcggc ggcggaatcg
     3361 tcggccttgc ggtgatggga cagacgctca acctctacag ccaggtcggc atcgtcatgc
     3421 tggtcggcct cgccgccaag aacggcatcc tcatcgtcga gttcgcaaac cagctgcgcg
     3481 acgccggccg cgacatcggc gaggcgatcc gggaagcctc ggcccggcgc ctgaggccga
     3541 tcctgatgac ctcgatcgcc accgcggccg gcgcggttcc gctgatgatc gccagcggcg
     3601 cgggtgccgg cgctcgccag gcgatcggcg tcgtcgtcgt cttcggagtt tcgatcgcga
     3661 cggtgatcac cctgttcctc atcccggtcc tttattcccg cctcgcccgc tacaccggct
     3721 cgccgctggc ggtgacgagg aagctggagg agcaattgag gcgtccgggc cacgcgcatc
     3781 cggcgccggc ggaatgaggt gcccgtcatt gcgagcgaag
//
LOCUS       DASVQZ010000141         2697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_30083, whole genome shotgun sequence.
ACCESSION   DASVQZ010000141 DASVQZ010000000
VERSION     DASVQZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30083"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2697)
                     /locus_tag="VF547_03970"
     CDS             complement(<1..>2697)
                     /locus_tag="VF547_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8262013.1"
                     /translation="EAAAAQDEAAAEADAQPAPDSTIVITGTRRTDRTVADSPVPVDV
                     IGSEAILNSGQTETNKILNQLVPSFNFPQPSIADGSDALRPATLRGLSPDQTLVLING
                     KRRHVSSLLNINGTVGRGSAAVDMNLIPGLAIGRVEVLRDGAAAQYGSDAIAGVINIQ
                     LKNSSSGGRASVTWGKYYTTVDNVANVNGLQLTGGGQPQLDPSDNRYLLANSDGDREA
                     HDGRQLTVGANIGIPMGPNGYFNVTGEFRDRDRTNRAGFDLRPNYNRPAPPAFDAREV
                     GFNRLQFRFGDPDSVDYNFFVNAGLELGGDWEMYGFGSYGHRDTVSAANYRQAGAVQN
                     RDFSALAPNQSPNAANFVSLTPDGFLPLIDTDLDDWAGTIGLRTDFGGWKADLSYGHG
                     QNSFDYTVRNSLNTSFGPQSQRVFDAGGLFYAQDIVNLDFSKEFAIGGFAKPLSVAAG
                     AEYRNEHFEIHPGDLQSYATGPFFRPSITTTAANCTAQQGVFNAGSGVCSFPGRAAPA
                     GAQGFPGIPGTSATDVGRHSWAAYVELDTDPIEGVTTTIAGRYEHFSDFGSTWNGKFA
                     ARWEPVEGFALRGSISNGFRAPSLHQQFFSTTSTNFINGVPVDIATLPVSAPAALALG
                     ARDLEPEKSLNMSLGVTANPLRGLTVTADLYQIDIDDRIVLTETLGTGGTGNTAAVQS
                     AVTALLASSGFPQVAAARFFINGLDTRTRGLDVVAVYRRNFEGLGNWSLSAAYNYNKT
                     RIQERLATLGPLATIPGLVLFGRVEGLRFTHGQPRDKIVFSADGDIGNLGITARTTRY
                     GKVVSPGAQAPLADPLSLTAFGPDDIFLKAKWISDLEVRYKVGGRLELALGANNLFDV
                     YPTRSPFGARPASVGGVYPANQQYIPFSIFSPFGFNGRFVYGR"
BASE COUNT          422 a          784 c         1037 g          454 t
ORIGIN      
        1 ccggccgtag acgaagcggc cgttgaagcc gaagggcgag aagatcgaga aggggatata
       61 ttgctggttc gcgggataga ccccgccgac cgaggccggc cgcgctccga agggcgagcg
      121 ggtcgggtag acgtcgaaca ggttgttggc gccgagcgcg agctccagcc gcccgccgac
      181 cttgtagcgg acctcgagat cgctgatcca cttggccttg aggaagatgt cgtccggacc
      241 gaaggcggtg aggctgagcg ggtccgcaag cggcgcctgg gcgccgggcg agacgacctt
      301 tccgtagcgc gtggtgcggg ccgtgatccc cagatttccg atgtcgccgt cggcgctgaa
      361 cacgatcttg tcgcgcggct ggccgtgggt gaaccgaagc ccctcgaccc gcccgaacag
      421 gacgaggccg gggatggtgg cgagcggccc gagcgtggcg aggcgctcct ggatccgggt
      481 cttgttgtaa ttgtaggccg cgctcagcga ccaattgccg agaccttcga aattccgccg
      541 gtagacggcg accacgtcga ggccgcgggt ccgggtgtcg aggccgttga tgaagaagcg
      601 tgcggcggcg acctgcggga agccgctcga ggcgagcagt gcggtgacgg cggactggac
      661 cgccgccgtg ttgccggttc cgccggtgcc gagggtctcg gtcaggacga tccggtcgtc
      721 gatgtcgatc tggtagagat cggcggtgac ggtgaggccg cgaagcgggt tggcggtgac
      781 tccgaggctc atgttgagcg acttttccgg ctccaggtcg cgggcgccga gcgccagcgc
      841 cgccggggcg ctgaccggaa gggtcgcgat gtcgaccggg acgccgttga tgaagttggt
      901 cgaggtggtg gaaaagaatt gctggtggag cgacggggcc cggaagccgt tcgagatcga
      961 gccgcgaagc gcgaagccct cgacgggctc ccagcgcgcc gcgaacttgc cgttccaggt
     1021 cgagccgaag tcggagaaat gctcgtagcg gccggcgatg gtggtggtga ctccttcgat
     1081 cggatccgtg tcgagctcga cataggccgc ccagctgtgc cggccgacgt cggtggcgct
     1141 ggtgccgggg attccgggga agccctgagc gcccgccgga gcggcccggc ccgggaagct
     1201 gcacactccg gagccggcgt tgaagacgcc ctgctgggcg gtgcaattgg ccgcggtcgt
     1261 ggtgatggac gggcggaaga agggtccggt ggcgtaggat tggagatcgc ccgggtggat
     1321 ctcgaaatgc tcgttgcgat attccgcgcc ggcggcgacg gagaggggct tggcgaagcc
     1381 gccgatcgcg aactccttcg agaaatcgag attgacgatg tcctgggcgt agaacagtcc
     1441 gccggcgtcg aacacgcgct ggctctgcgg tccgaacgag gtgttgagcg aattgcggac
     1501 ggtatagtcg aagctgttct ggccgtgacc gtaggagagg tcagccttcc agccgccgaa
     1561 gtcggtcctc aggccgatgg tgccggccca gtcgtcgaga tccgtgtcga tcagcggaag
     1621 gaagccgtcc ggagtgagtg aaacgaaatt ggcggcattg ggcgattggt tcggagccaa
     1681 ggcgctgaaa tcgcgattct gcaccgctcc ggcctgccgg tagttggcgg cgctgaccgt
     1741 gtcgcggtgg ccgtaggagc cgaagccgta catttcccag tccccgccaa gctcgagccc
     1801 ggcattgacg aagaaattat agtcgaccga atcgggatcg ccgaagcgga actggaggcg
     1861 gttgaagccg acctcgcgag cgtcgaacgc cggcggagcc gggcggttgt aattgggcct
     1921 caggtcgaag ccggcgcgat tggtccggtc gcggtcgcgg aattcgccgg tgacgttgaa
     1981 atagccgttc ggccccatcg ggatcccgat attggccccg acggtcagct gacggccgtc
     2041 atgggcctcc cggtcgccgt cgctgttggc gaggagatag cggttgtcgg acggatcgag
     2101 ctggggctgg ccgccgccgg tgagctgcag tccgttgacg ttggcgacgt tgtcgacggt
     2161 cgtgtaatat ttgccccagg tgaccgaggc ccggccgccg ctgctcgaat tcttgagctg
     2221 gatgttgatc acgcccgcaa tggcatcgga gccgtactgc gccgcggcgc cgtcccggag
     2281 gacctcgact cggccgatgg cgaggccggg gatgaggttc atgtcgaccg ccgcgcttcc
     2341 ccgcccgacc gttccgttga tgttgaggag cgacgagacg tggcggcgct tgccgttgat
     2401 caggaccagg gtctggtccg ggctgagccc ccggagggtg gcagggcgga gcgcgtccga
     2461 gccgtcggcg atggagggct gcgggaagtt gaagctcggc accagctggt tgaggatctt
     2521 gttggtctcg gtctggccgg aattgaggat cgcctccgag ccgatgacgt cgaccggaac
     2581 cgggctgtcg gcgaccgtcc tgtcggttcg gcgggtgccg gtgatgacga tggtggaatc
     2641 gggcgccggc tgggcgtccg cctcggcggc ggcctcgtcc tgcgcggccg cggcctc
//
LOCUS       DASVQZ010000142         5541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_800175, whole genome shotgun sequence.
ACCESSION   DASVQZ010000142 DASVQZ010000000
VERSION     DASVQZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800175"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VF547_03975"
     CDS             <1..355
                     /locus_tag="VF547_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8262014.1"
                     /translation="EPVPTDFGTEGGGLLAMRPRNFYASSSDLVDLEGELEGMVDRYP
                     TISVPVSILYAPGDNLLSSQKHGDRTASEIPGAEIELVEGGHMLPFTQPELTAAFVRR
                     AAARQEEPHARRAAG"
     gene            333..1997
                     /locus_tag="VF547_03980"
     CDS             333..1997
                     /locus_tag="VF547_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8262015.1"
                     /translation="MPDALQADYIVVGAGSAGCVLAARLSEDGRARVLLLEAGGDDRP
                     LKQPSQFMSNVWIHVPVGYSQTLKDPKVNWLYTTEPDAGTHGRTHVWPRGKVLGGSSS
                     INAMLYIRGQQADYDGWRQLGCEGWAWDDVLPYFRRCQHQERGECDLHGVGGPLNVSD
                     PTTGHEVSEAVIEACEALGLPHRDPNGPEQEGVGWYQVTIKDGKRCSAAVAYLHPAMG
                     RPNLRVETRALATRVLFDGRRAVGVEFVQDGVTRVARADAEVILAGGAINSPQLLQLS
                     GIGPGALLREHGVELVADLPGVGENLQDHFVIGETFRLKKGVVSVNELVRGTRFLKET
                     LKYMFGKKGLLTLSAAHVAVFCKSRPDLAGPDIQFHILPATMDPDKLANAQKMELEKE
                     PGLTIAPCQLRPESRGTIRIKSPDPAVHPAIAPNYLSHRLDQEVAVAGLRWGRRIAEQ
                     PALARFIERELMPGPDAATDEQLLDHARTWGTTIYHPVGTCAMGHGPMAVVDPRLRVH
                     GVEALRVVDASVMPRLVSGNTNAPTVMIAEKASDMIRGQVLAAAAA"
     gene            complement(2129..3562)
                     /locus_tag="VF547_03985"
     CDS             complement(2129..3562)
                     /locus_tag="VF547_03985"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEX8262016.1"
                     /translation="MADHNVARPVAGEDEARRLFDLQHRESRRQPAADLKLRRDRLER
                     LRAVLAGREAAFAKAISDDFGVRSRTETELLEIVPTMSAIRHARKQVERWMRPERRRV
                     DPLFQPAAAWVRFEPLGVIGIISPWNYPLQLAFSPLVDALAAGNRAMVKPSELTPAFS
                     ELLRGAVAERFDEAEVAVVTGGVEVGQAFAGLPFDHLLFTGSTAIGRKVYQAAAANLV
                     PVTLELGGKSPAVVCPDYDLGKAARSVAFGKFVNAGQTCIAPDYVLVPQGREREFAEA
                     VMAQVRRSYPTIADNDDYSGLISDRHRRRLIDALEAARVAGATVLAHEDEGARAKGKI
                     GPTVVLGAAEDSLFLNEEIFGPVLPVLPYRGLDEAVAFIAARERPLALYCFSHRRRDR
                     EQVLGGAISGGVTLNGTLLHVAQENLPFGGVGASGIGAYHGREGFRRFSHARAVHKVG
                     FVNVFEKLGPPWGKMAAALGRRLAKRR"
     gene            3790..4710
                     /locus_tag="VF547_03990"
     CDS             3790..4710
                     /locus_tag="VF547_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8262017.1"
                     /translation="MPIRIAIVGYGKIARDQHVPSIAADPRFELAAIVSPRARADEGV
                     PVFASAGTMLEQMGGKLDAVAVCTPPTVRLEIAAAAFGAGLAVMLEKPPAATLGEIEE
                     IERLGRAAGRTLFTAWHSQHAPGVAAAKAALAGQRVRSLDILWHEDVRKWHPGQEWIW
                     APGGFGVFDPGINALSIATRILPSRLFVREAVLAFPENRQTPIAARIGFVGGFEADLD
                     WRHAEGERWTIRVETGDGRVVELLDGGARLKVDGVPQEVGSAGEYPSLYARFAELVEA
                     GESDVDREPLRLVADAFLAGGRETVEPFVW"
     gene            4898..>5541
                     /locus_tag="VF547_03995"
     CDS             4898..>5541
                     /locus_tag="VF547_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX8262018.1"
                     /translation="MEEIGRGDAGTRNLTFGLLETLGRAIVTGAYESERFPTEAELAT
                     QHAVSRSVTREAVKMLTAKGLLTARPRKGTTVQPPSCWNLFDTDVLRWLLEREFSLDL
                     LRQFSELRIAIEPEAAALAARKADPESVAAIEAGYARMEAAEAGADDALESDIAFHIA
                     ILEACGNPFYRQFRDVVETALRTSIRFTNRFKGRTASLPAHHAVLAAVRARNPDG"
BASE COUNT          857 a         1979 c         1931 g          774 t
ORIGIN      
        1 cgagccggtg ccgaccgact tcggaacgga gggcggcggc ctgctcgcca tgcgcccgcg
       61 caacttctac gcctcctcga gcgacctcgt cgatctcgag ggcgagctgg aggggatggt
      121 cgatcgctac cccacgatct cggttccggt cagcatcctc tacgcgcccg gcgacaatct
      181 gctgagctcg cagaagcatg gcgaccgcac ggcgagcgag ataccgggcg ccgagatcga
      241 gctggtcgag ggcgggcaca tgctgccctt cacccagccc gagctcaccg ccgccttcgt
      301 ccgccgcgcg gcggcgcgcc aagaggaacc gcatgcccga cgcgctgcag gctgattata
      361 tcgtcgtcgg cgccggatcg gccggctgcg tgctcgcggc gcgcctcagc gaggacggac
      421 gcgcccgcgt gctgctgctc gaggcgggcg gcgacgatcg gccgctgaag cagccgtccc
      481 agttcatgtc gaacgtctgg atccacgttc cggtgggcta ttcgcagacc ctgaaggacc
      541 cgaaggtcaa ctggctctac acgaccgagc cggacgccgg gacccacggc cgcacccatg
      601 tctggccgcg gggcaaggtg ctcggcggat cgtcctcgat caacgccatg ctctacataa
      661 ggggccagca agccgactat gacggctggc gccagctcgg ctgcgagggc tgggcgtggg
      721 acgacgtcct cccctatttc cgccgctgcc agcaccagga gcgcggcgag tgcgacctgc
      781 acggcgtcgg cggcccgctg aacgtctccg atccgaccac gggccatgaa gtctccgaag
      841 cggtgatcga ggcgtgcgaa gcgctcggcc tgccccaccg cgacccgaac ggcccggagc
      901 aggagggggt cggctggtac caagtgacga tcaaggacgg caagcgctgc tcggccgccg
      961 tcgcctatct ccatccggcc atgggccggc ccaacctccg ggtcgagacc cgggcgctgg
     1021 cgacccgcgt gctgttcgac ggcaggcggg cggtcggcgt cgagttcgtg caggacgggg
     1081 tcacccgggt cgcccgggcc gacgcggagg tgatcctcgc cggcggcgcg atcaattcgc
     1141 cccagctgct gcagctgtcc ggaatcggac cgggcgccct gctgcgcgag cacggcgtcg
     1201 agttggtcgc cgacctgccc ggcgtcggcg agaacctcca ggaccatttc gtcatcggcg
     1261 agacgttccg gctgaagaag ggcgtcgtct cggtgaacga gctggtccgc ggcacccgct
     1321 tcctcaagga gacgctcaaa tacatgttcg gcaagaaggg cctgctcacc ctctcggccg
     1381 cccatgtcgc cgtcttctgc aagtcgcggc ccgatctcgc cggccccgac atccaattcc
     1441 acatcctccc cgcgacgatg gaccccgaca agctcgccaa cgcccagaag atggagcttg
     1501 agaaggagcc gggcctgacc atcgcgccct gccagctgcg ccccgaatcg cgcggcacga
     1561 tccggatcaa gtcgcccgat ccggccgtcc atccggcgat agcgcccaat tacctgtccc
     1621 atcgcctcga ccaggaggtg gctgtcgccg ggctgcgctg gggccggcgg atcgccgagc
     1681 agccggccct cgcccgcttc atcgagcgcg agctgatgcc cggtcccgac gcggcgaccg
     1741 acgagcaatt gctcgatcat gcccggacct gggggacgac catctaccat ccggtcggaa
     1801 cctgcgcgat gggccatggg ccgatggcgg tggtcgatcc gcggctcagg gtgcacggag
     1861 tggaggcgct gcgggtggtc gacgcctcgg tgatgccgag gctggtgtcc ggcaacacca
     1921 acgccccgac ggtcatgatc gcggagaagg cgagcgacat gattcgggga caggtgctgg
     1981 cggcagcggc tgcctgaatt cctccccgcg atttcgcggg gagggggacc aggcgaagcc
     2041 tggtggaggg gttctacgac cgaaaaaccc ctccacccgc ttcgcgcggt ccccctcccc
     2101 cgcaaaagcg ggggaggaat tccgcccgct accgccgttt cgccagcctc cggcccagcg
     2161 ccgcggccat cttgccccac ggggggccga gcttctcgaa gacgttgacg aagccgacct
     2221 tgtgcacggc tcgggcatgg ctgaagcgcc ggaagccttc ccggccgtga taggcgccga
     2281 tgccgctggc gccgacgccg ccgaacggga ggttctcctg ggcgacgtgg agcaaggtgc
     2341 cgttgagcgt cactccgccg gaaatcgcgc ccccgagcac ctgctcgcgg tcgcggcggc
     2401 gatggctgaa gcaataaagc gccagcggcc gctcacgcgc cgcgatgaag gcgacggcct
     2461 cgtcgagccc gcgatagggc aggaccggca ggaccggccc gaatatctcc tcgttgagga
     2521 acagcgagtc ctccgccgcg cccaggacga cggtcggtcc gatcttgccc ttcgcccggg
     2581 ccccctcgtc ctcgtgggcg aggacggtgg cgcccgcgac gcgcgccgcc tcgagggcgt
     2641 cgatgagccg gcgccggtgg cgatcgctga tcaggcccga ataatcgtca ttgtcggcaa
     2701 tggtcggata ggaacggcgc acctgcgcca tcaccgcctc ggcgaactcc cgttcccgcc
     2761 cctgcggcac gagcacatag tccggtgcga tgcaggtctg gccggcgttg acgaacttgc
     2821 cgaacgcgac gctgcgcgcc gccttgccga gatcgtagtc gggacagacc acggccggcg
     2881 acttgccgcc aagctcgagc gtcaccggaa ccaggttcgc ggcggccgcc tggtagacct
     2941 tgcggccgat cgcggtcgag ccggtgaaca gcagatggtc gaacggcagt ccggcgaaag
     3001 cctggccgac ctccaccccg ccggtcacga cggcgacctc ggcctcgtcg aaccgctcgg
     3061 cgacggcgcc gcgcaggagc tcggagaagg ccggcgtcag ctcggacggc ttgaccatcg
     3121 cccgattgcc tgcggcgagg gcgtcgacca gcggcgagaa ggcgagctgc agcggatagt
     3181 tccacggcga gatgatcccg atcacgccga gcggctcgaa ccgcacccag gcggcggcgg
     3241 gctggaacag gggatcgacg cgccgccgct ccggacgcat ccagcgctcg acctgctttc
     3301 gggcatggcg gatcgcgctc atcgtcggca cgatctcgag caattcggtc tcggtgcgcg
     3361 agcgcactcc gaaatcgtcc gaaatcgcct tggcgaaggc ggcttcgcgg ccggccagga
     3421 cggcgcgcag ccgctccagc cggtcccgcc gaagcttgag gtcggccgcc ggctggcggc
     3481 gcgactcccg gtgctggagg tcgaagaggc gccgcgcctc gtcttcgccc gcgaccggcc
     3541 tcgcgacatt gtgatcggcc atgctgcgtc ctccctcgaa gccccgcata gccgatcgac
     3601 gcgcccgtcg catcctttgc cgcctcggcc ggttatcgcg gccggctcca atcatgtctc
     3661 gcaccacccc gaccgggccg ccggcacgtc cggcgccggc ccgaccggcg agggaacggg
     3721 cccgggcggc aacgccctcg ccgagagcgg cgcggaccgc taaggcgtcg ggaaacgagg
     3781 gagcgcagca tgccgatccg aatcgccatc gtcggctacg gcaagatcgc ccgggaccag
     3841 cacgtcccgt cgatcgccgc cgatccgcgc ttcgagctcg ccgcgatcgt cagcccgcgc
     3901 gcccgcgccg acgagggcgt gcccgtcttc gcgagcgccg ggacgatgct ggagcagatg
     3961 gggggaaagc tcgacgcggt cgcagtctgc acgccgccga cggtcaggct cgaaattgcc
     4021 gcggccgcgt tcggagccgg gctggcggtg atgctcgaga agccgcccgc cgcgaccttg
     4081 ggcgagatcg aggagatcga gcggctcggc cgcgcggccg ggcgcaccct gttcaccgcc
     4141 tggcattcgc aacatgcgcc cggcgtcgcc gccgccaagg cggccttggc cggccagcgg
     4201 gtgcgatcgc tcgacatcct ctggcacgag gacgtgcgca aatggcatcc cggacaggaa
     4261 tggatctggg cgccgggcgg cttcggagtg ttcgaccccg gcatcaacgc cttgtcgatc
     4321 gcgacccgga tcctcccctc tcggctgttc gtccgcgagg ccgttctggc cttccccgag
     4381 aaccgccaga ctccgatcgc ggcgcgtatc ggtttcgtcg gcgggttcga ggcggacctg
     4441 gactggcggc atgccgaggg cgagcgctgg acgatccggg tcgagaccgg cgacggccgg
     4501 gtggtcgagc tgctcgacgg cggcgcccgc ctcaaggtcg acggagtccc gcaggaggtc
     4561 ggctcggccg gcgaatatcc gtcactctac gcgcgcttcg ccgagctggt cgaagccggc
     4621 gagagcgacg tcgaccgcga gccgctgcgc ctcgtcgccg acgccttcct ggccggcggc
     4681 cgcgaaacgg tggagccgtt cgtgtggtga ccgtcgtccc ggggacggga gcgaccgcga
     4741 ggcagcgtcg aaccctatcg ggatcgggac gaagcccgtt cgacggccgc aaccggcacg
     4801 gcggtcccgc cctgcgcggc gaggttgccg ctcggacaaa ggaattatag gatcaaactg
     4861 ccctgtccag cgggcagtgc ggggaaggat ggcgagcgtg gaggaaatag ggcgcggcga
     4921 cgcagggacc cgcaatctga ccttcggctt gctggagacg ctcggacgcg cgatcgtcac
     4981 cggggcctat gagagtgagc gcttcccgac cgaggccgag ctggcgactc agcatgcagt
     5041 gagccgctcg gtcacccggg aggcggtgaa gatgctgacc gccaagggcc tgcttaccgc
     5101 ccgcccccgc aaggggacca cggtgcaacc gccgtcctgc tggaacctgt tcgataccga
     5161 cgtgctgcgc tggcttctcg agcgcgaatt ctcgctcgac ctgctgcgcc agttcagcga
     5221 gctccgcatc gcgatcgagc cggaggccgc ggccctcgcc gcccgcaagg cggatccgga
     5281 gtcagtcgcc gccatcgagg cgggctacgc gcgcatggag gcggccgagg ccggcgccga
     5341 cgacgcgctg gaatcggaca ttgccttcca catcgccata ctggaagcct gcggcaaccc
     5401 cttctaccgc cagttccgcg acgtggtcga aaccgcgttg cgcacgtcga tccgcttcac
     5461 caaccgcttc aaggggcgga ccgccagcct tcccgcccac catgcggtgc tcgccgcggt
     5521 gcgggcccgc aaccccgacg g
//
LOCUS       DASVQZ010000143         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_510429, whole genome shotgun sequence.
ACCESSION   DASVQZ010000143 DASVQZ010000000
VERSION     DASVQZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510429"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF547_04000"
     CDS             <1..259
                     /locus_tag="VF547_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX8262019.1"
                     /translation="EDHRYAPYVERQQAEVARLRADEAVRIPADLDFAAVPGLSAEMV
                     ERLSAARPDTLGAARRLRGITPAALAAILVHSRRVSTRRAA"
     gene            269..895
                     /gene="rsmG"
                     /locus_tag="VF547_04005"
     CDS             269..895
                     /gene="rsmG"
                     /locus_tag="VF547_04005"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX8262020.1"
                     /translation="MNEIEARAALDVPRETLERLEAFVALLQSENGRQNLVSRSSLDQ
                     LWQRHILDSAQLARFAPPDADIWLDLGSGAGFPGLLIPRFHPASVVLVESRRLRVDFL
                     RAAASVLGVGARVEIVGARLETVPARPVEVISARAFAPLPKLLALAERFSRPETVWIL
                     PKGRNAKSELDAARSSWQGDFRLEPSLTDADAAIIVATGVRRITRGRR"
     gene            892..1671
                     /locus_tag="VF547_04010"
     CDS             892..1671
                     /locus_tag="VF547_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8262021.1"
                     /translation="MIRIAVANQKGGVGKTTTAINLATALAATGWRVLLIDLDPQGNA
                     STGLGVGQALRDRSSYEVLTGEATAEEAVVATKVPRLDLVPATVDLSGAEIELVALEE
                     RTHRLDKALDEAPARWDVCLIDCPPSLGLLTINALVAAQSILVPLQTEFFALEGLSQL
                     LQTIERIRARFNPELSILGVALTMFDRRNNLSSQVADDVRACLGKVVFDTVIPRNVRL
                     SEAPSHGIPALIYDHRCPGSEAYMALARELIGRLPKKAAAA"
     gene            1668..2561
                     /locus_tag="VF547_04015"
     CDS             1668..2561
                     /locus_tag="VF547_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8262022.1"
                     /translation="MSDDSPRKRPSGLGRGLSSLLGEVAQEAPVAGGAAPARGGVQMM
                     PISSIEPHLGQPRRHFDEEALEELAESIKARGLIQPIVVRPHGHRYQIVAGERRWRAA
                     QRARLHEIPVIVRDFDDAETLEVALLENIQRRDLNAIEEAEGYKRLIDEYGHTQEALG
                     KLVHKSRSHVANLLRLLDLPEGVRQLVESGGLSMGHARALIGAADPEALARDVVERGL
                     SVRETEKLARFGKPGRERQGRLEIKGAGADIEALERQLGDMLGLKVKITYRPDGGTVA
                     LNYSTLDQLDMICQRLSGEKI"
     gene            complement(2574..>2693)
                     /locus_tag="VF547_04020"
     CDS             complement(2574..>2693)
                     /locus_tag="VF547_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX8262023.1"
                     /translation="RKAGERTTEAVWRRIGSLLDTFTPSECANYIRNSGYASC"
BASE COUNT          412 a          938 c          939 g          404 t
ORIGIN      
        1 cgaggatcat cgctacgcgc cctatgtcga gcggcagcag gccgaggtcg cccgcctccg
       61 cgccgacgaa gcggtgcgca tccccgccga tctggacttc gcagcggtgc cgggcctttc
      121 ggccgagatg gtagagaggc tcagcgcggc ccggcccgac acgctcggcg cggcacggcg
      181 gctgcgcggc atcaccccgg cggccctcgc cgcgatcctc gtccactccc gccgcgtttc
      241 gacgcgcaga gcggcgtaag aagcgtagat gaacgaaatc gaggctcgcg cggcgctcga
      301 tgttccacgt gaaacactgg agcgcctgga ggcgttcgtg gccctcctgc agagtgagaa
      361 cgggcgtcag aacctggtgt cgcggagctc gctggaccag ctctggcagc gtcatatcct
      421 ggattcggcg caactggcgc gattcgcccc gccagacgcg gacatctggc tcgacctggg
      481 cagcggcgcc ggcttcccgg ggctcctcat cccccgattt cacccggcct ccgtcgtgct
      541 ggtcgagtcc cgccggctcc gtgtggattt cctccgcgcc gcagcgtcgg tgctcggcgt
      601 cggcgccagg gtcgagatag tcggcgcccg gctcgagacc gtgccggcgc gtccggtgga
      661 ggtgatcagc gcccgcgcct tcgcgccgtt gccgaagctg ctggccctcg ccgagcgttt
      721 ttcgcggccc gaaacggtct ggatcctccc caaaggccgc aacgccaagt ccgaactgga
      781 tgcggcgcga tcttcgtggc agggtgattt ccgtctcgaa ccgagcctca ccgacgccga
      841 tgccgcgatc atcgtcgcaa cgggggttcg ccgaataacg aggggaagac gttgatccgg
      901 atcgccgtgg ccaatcagaa aggcggggtc ggcaagacga cgacggcgat caacctcgcc
      961 accgcgctcg ccgcgaccgg ctggcgggtg ctcctgatcg acctcgaccc gcagggcaat
     1021 gcctcgaccg gcctcggcgt cggccaggcc ctgcgcgatc ggtcgagcta cgaggtgctg
     1081 accggcgaag cgacggcgga ggaggcggtg gtggcgacca aggtgccccg gctcgacctg
     1141 gtgccggcga cggtcgacct gtcgggggcg gagatcgagc tggtggcgct cgaggagcgg
     1201 acccaccggc tcgacaaggc gctcgacgag gcgccggcgc ggtgggacgt ctgcctgatc
     1261 gactgcccgc cgagcctcgg cctgctgacc atcaacgcgc tcgtcgcggc ccagtcgatc
     1321 ctggttccgc tccagaccga gttcttcgcc ctcgagggcc tgagccagct cctccagacg
     1381 atcgagcgga tccgcgcgcg gttcaatccg gagctgtcca tcctcggggt ggcgctgacc
     1441 atgttcgatc gccgcaacaa cctctcctcg caggtcgccg acgacgtccg cgcctgtctc
     1501 ggcaaggtgg tgttcgacac ggtcataccg cgcaacgtcc gcctctcgga ggcgccgagc
     1561 cacggcatcc ccgccctgat ctacgaccac cgctgcccgg gcagcgaggc ctatatggcg
     1621 ctcgcccgcg agctgatcgg ccgcctgccc aagaaggccg ccgcggcatg agcgacgact
     1681 cgccgcgcaa gcgtccctcg ggcctcggcc gcggcctttc gtcgctgctc ggcgaggtcg
     1741 cgcaggaggc gccggtggcc ggcggcgccg cgcccgcccg cggcggagtc cagatgatgc
     1801 cgatctcgtc gatcgagccg catctcggcc agccccgccg gcatttcgac gaggaggcgc
     1861 tggaggagct cgccgagtcg atcaaggcgc gcgggctgat ccagccgatc gtcgtccggc
     1921 cgcacggcca ccgctaccag atcgtcgccg gcgagcggcg ctggcgggcg gcccagcggg
     1981 ctcggctcca cgaaatcccg gtgatcgtcc gcgacttcga cgacgccgag acgctcgagg
     2041 tggctctgct cgagaacatc cagcgccggg acctcaacgc gatcgaggaa gcggagggct
     2101 acaagcggct gatcgacgaa tatggccaca cccaggaggc gctcggcaag ctcgtccaca
     2161 agagccggag ccatgtcgcc aacctcctga gattgctcga tcttcccgaa ggcgttcgcc
     2221 agctggtgga gagcggcggg ctgagcatgg gccatgcccg cgcactgatc ggcgcggccg
     2281 acccggaagc gctcgcccgc gacgtcgtcg agcgcggcct gtcggtgcgc gaaaccgaga
     2341 agctggcccg gttcggcaag ccgggccgag agcgccaggg acggctcgag atcaagggcg
     2401 cgggcgccga catcgaggcg ctcgagcgcc agctcggcga catgctcggg ttgaaggtca
     2461 agatcaccta ccgccccgac ggcggcaccg ttgcgctcaa ctattcgacg ctcgaccagc
     2521 tcgacatgat ctgccagcgg ctgagcgggg agaagatcta gagcatgatc gtcttagcat
     2581 gaggcgtatc cgctgtttct gatgtagttg gcgcattcgc tgggtgtgaa ggtgtcgagg
     2641 agggatccga ttcggcgcca gacggcttcg gtggtgcgtt cgccggcctt gcg
//
LOCUS       DASVQZ010000144         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_129793, whole genome shotgun sequence.
ACCESSION   DASVQZ010000144 DASVQZ010000000
VERSION     DASVQZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129793"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF547_04025"
     CDS             <1..332
                     /locus_tag="VF547_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX8262024.1"
                     /translation="EAADEEAAPPGRANWFLRGLLTNLLNPKVGAFYVSFLPQFIPAS
                     ANVASTTLLLAAIHAALGLVWFLLLIGATRPLARVLRSARVVRALDRLTGGVLIFFGL
                     KLALSRQ"
     gene            356..973
                     /locus_tag="VF547_04030"
     CDS             356..973
                     /locus_tag="VF547_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262025.1"
                     /translation="MTSRIATGAGHALVFLAAAAIGLQPLAAQREVVAEGPYLHGPAR
                     AVLPVAVGEFRRSRIFQYDAEGKDVSASYDLATPNGRLLITVYIYPAAAAAGESEAVH
                     CRREFEEANKAVSSQHGHVAADERGPAMAVDGIDRALGHRALYRFSSPFDDRVQAIRS
                     EVHLYCYAGGDWLVKYRVSAPAAVETRGPVEAFIRHGPWPGRSSS"
     gene            1052..1255
                     /locus_tag="VF547_04035"
     CDS             1052..1255
                     /locus_tag="VF547_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262026.1"
                     /translation="MSEPDRERETTIVHTDSGGGGGGTIVAVVLLLAVIVLLFYLFGG
                     QLLGGADKTTDVKVDVTAPAKSS"
     gene            1302..1616
                     /locus_tag="VF547_04040"
     CDS             1302..1616
                     /locus_tag="VF547_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262027.1"
                     /translation="MSDPDRERETVRDVERTTTVVQTGNGDRGGGGVLLAVLVILVLL
                     AVLYFVFSGSFNRAADEVGVNVAVEAPKVDIPDVNVKMPDKIEVKVPDEVKVDTDGNE
                     AR"
BASE COUNT          281 a          608 c          599 g          255 t
ORIGIN      
        1 aggaagccgc cgacgaggag gcggcgcccc ccggccgggc caactggttc ctgcgcggcc
       61 tgctgaccaa cctgctcaat cccaaggtcg gcgccttcta cgtctcgttc ctgccgcagt
      121 tcatcccggc gtcggcgaac gtggcctcga ccaccctgct tctcgccgcc atccacgccg
      181 cccttgggct ggtctggttc ctgctgctga tcggagccac ccggccgctg gcgcgggtgc
      241 tgcgaagcgc gagggtggtc cgggcgctcg accggctgac cggcggagtg ctcatcttct
      301 tcgggctgaa gctggcgctc agccggcaat gaccgcgatt gcgaagggac gcttcatgac
      361 ctcgaggatc gcgaccggcg ccggacacgc tctcgttttc ctggcggcgg cggcgatcgg
      421 cctacaaccg ctcgccgcgc agcgggaggt cgtcgccgaa ggcccctatc tgcacgggcc
      481 ggcgcgggcc gtgctgcccg tcgccgtggg cgagttccgc cgctccagga tcttccagta
      541 cgacgcggag ggcaaggacg tcagcgcgag ctacgacctc gccactccga acggacggct
      601 tctgatcacc gtctacatct atcccgcggc ggcggccgcc ggcgagtccg aggccgtgca
      661 ctgccgccgg gaattcgagg aggcgaacaa ggccgtgtcc agccagcacg gccatgtcgc
      721 cgcggacgag cgagggccgg ccatggcggt cgacgggatc gatcgcgcgc tcggccatcg
      781 cgccctctac cgcttcagca gcccgttcga cgatcgcgtc caggcgatac gctcggaggt
      841 gcacctctat tgctatgccg gcggcgactg gctggtgaaa tatcgggtca gcgccccggc
      901 cgcggtcgag acccgcgggc ccgtcgaggc gttcatccgc cacggcccct ggcccggccg
      961 gtcgtcgagc tagccggaat tcgtcggcga ccgcggagga acccccctcc cggcgcagcg
     1021 ttgaggttcc gaaacaaagc ggagacatgc catgagcgag cctgaccggg agcgcgaaac
     1081 cacgatcgtc cataccgaca gcggcggcgg gggcggcggc acgatcgtcg cggtcgtcct
     1141 gcttctggcc gtgatcgtcc tcctcttcta tttgttcggc ggccagcttc tcggcggcgc
     1201 cgacaagacg accgacgtca aggtggacgt gaccgcgccg gcgaagagca gctgaactgc
     1261 cggcggccgg cctggccggc gccgcaacca aggagaatga catgagcgac cccgatcgcg
     1321 agcgcgagac cgtgcgcgac gtcgagcgga ccacgaccgt cgtccagacc ggcaacggcg
     1381 accgcggagg cggcggcgtg ctgctcgccg tcctggtcat cctcgtgctg ctggccgtgc
     1441 tctatttcgt gttcagcggc agcttcaacc gggccgccga cgaagtcggg gtgaacgtcg
     1501 ccgtcgaggc gcccaaggtg gacatccccg acgtcaacgt gaagatgccc gacaagatcg
     1561 aggtcaaggt cccggacgaa gtgaaggtgg acaccgacgg caacgaagcc cgctaagaag
     1621 cggacggctt aacctcggct gtcgaagcca ttcagggatt cctcaaccga atcaagcgat
     1681 aaggaccggg tcgacccgcg ggccgacccc tcgagtcgcg taacgaggga ggggccggca
     1741 gcc
//
LOCUS       DASVQZ010000145         8009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_129865, whole genome shotgun sequence.
ACCESSION   DASVQZ010000145 DASVQZ010000000
VERSION     DASVQZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129865"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..6960)
                     /locus_tag="VF547_04045"
     CDS             complement(<1..6960)
                     /locus_tag="VF547_04045"
                     /inference="COORDINATES: protein motif:HMM:NF042460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HEX8262028.1"
                     /translation="MTESISMVNSIDREDSDRVRELQCALDALGFRIAGEERESASAG
                     RSTLQAVRSIERSAGLAIDERVVLSAAAAAEINRRLAGAGRIAGPAGGPAELLRVEGR
                     VRGSDGVAFAGLQVRVFDRDLRDRQLLGTASTDRSGDYAVAYEAGQFARAEKGYADIV
                     VQLLRPGADEPLYESEVLFNAPARLVHDIRLEMRGPSLYQRLIEAIEPLAEGQGVELA
                     EIDETERFRDVSFLAGETGFAAFEVAALAVAARLTAQTGLDPRLLFALVLSGVWPEPA
                     GTEPGAIRDLDARARALRARFGEPSMRAVRLALDTSVETGVVAATKAEIERFVRAFAD
                     LAGREGGSAPGAALPGTLLRQALGTDAAAIEGQLDKALDGASLVRSLRKDGQVSDRIV
                     RKVEGLVALNTAAFGDQALVLGLADKVESADSVRLLARLDARQWSRLIKDAKAEPPPF
                     VAEAGGKKADYAALLATRFRSLYPTQAFLGDAGRDRKAPQPLRDLAALIGDEPELDLA
                     NISIDRYLARSAGKRGPARGGRQPRRGTRAESLREMGESIKPLQRLYKISTDYSAVAT
                     LARHGLGSAQAVYRLGEDQAVRLLTDEAGLSERAAQLVFERAGATAAASLQLLVGVAS
                     ETQVLDVPVMSGLADEAQTVPELGSLFAMGNDCVCADCSSVLSPAAYLADLLLFLSNR
                     KLGDGRSAKDVLLARRPDLAWIDLNCPNAFTPLPYVDLAIEAMEDLVAPHSVGTLTAA
                     QRTTLPSDSADHAVPAAVVAAFSAFAPPIALSAAARVRKAGSSWVLRDGESGFRILDS
                     GAGPNVLVQRNTHGRPQDRELMPEYSNPHATAALNAKSFPWTLPLDLDGAETRLSLAK
                     LGIRRDDLMRALRGPAAPNNPTPFELAAATIGISSALASVLVTPAPNPSAHWGVGSVA
                     QAVAKYREVPTFLAVTGLSYADLLRLLSLPYANLNGALAIEHLDDSCDLAQKRLITLD
                     APSLDRLHRFLRLARALGLEAADTDLLIRRFGAGGALGNQQDLIAIADAMRVRSELGK
                     LPVEELVALFGAIPTEDRFVEAHEPPAPSLYRRLFLDPLVHRPVDPAFEIPAVTAAAP
                     AAQIAGHRSAILAALRIPSSDLDQLLPFAGQALSLANLSLLYRHARVARALDIPIAEW
                     TLFARLVGGDPFASPAALLGAMETIAAARALEIDAALLSHLLEARLDVAGALPAEAAG
                     AVLAGLRATLQAIAEQTQPPAPDSDRQTLIDRLPGAFAPLDPDSGRAALVAEMVENRF
                     VARTEISPVPAALPVLDPSIAKLPFSAVVDGGKLVLSYTGAIGPAVKSLLNDPAKVGP
                     VAGDVRWAPAIDALEQQPRLAVATVSTRFEAPLAAWPAGAALPAALKKRARYVAARRQ
                     LQFDGVMTAKERADLLAVSADPGWAAAVASLFDQPRATPVPAAQRLADFATWQFTDPT
                     TDDEVAADAKVRANLVAMLAVLLPRARAAASREAVVTAMTAVVELDRDSIAAVLDRVS
                     IAGATLMATLTDGPGFVATSGPVTPATLLPQFQAVDLLDRAARIAAQLRMGAREIDLV
                     QTLAAAPNSDLLGFQALPLAYAGPPGPPPAPPALSPLARLVRLGTLLLLDRKTARKPV
                     RLLDLMARILASPASVTLGPGPGTLDLFATAEESLGWPASEVGTLAAAGVGLGLAYPD
                     DFRNAAAWRRLERALAVVRRTRLSAADALKLARAAPTAAEAATARQAMRVRLGQQYAE
                     AGREVQDEMRALRRDGLRDFVLAYFQPGTGGRPPSPTGRWAKPTDVFAFLLIDPKMGA
                     CQMTSRIVQATIAIQTFVDRCFLGFEPEVSPTMAADGLWDHWKWMHAYRLWEANRKVF
                     LFPENWVEPELRRDKSYAFKELEDELLQTELTEDSARDAFLNYIDKLDQVANLDVTGV
                     HWQDGAGTGRGAVHVVGRTAGQEPHAYFYRRFDHPAERWTAWSKIELDLKSAPVVPFM
                     YDRRAFLVWPEFREVKPSTEATISIPGSGGGSAAVERPRVACGVALSELRNGKWSPKK
                     VSAQFLRPIDRDDYHERLAWTPVDLRRIAEDEHLYFQDIDARFYSSDGDCYEFLGCRG
                     YPEPTRNYYYFVTVTFHNMELRAARDVEAQEVGPLIAFGTSTYGGGTPVLAATPDKVR
                     ITYPHHFNSFDLNNLQALQALNWLFGTSLTFDRGDKYVVAGTRAPWIFADKRHSFFVE
                     PVTAFPDGRLLTGSSILDIWREWILSTPVFLQPFVRLFLEWLLWVDPFRRLRFSIFQH
                     PMVCAFREALLEGGVPKLLSRPTQFLKGELDFDDRYAPVNGVVLRDNTAGDPVYHPVE
                     DVDFSPRGAYSLYN"
     gene            complement(7033..7242)
                     /locus_tag="VF547_04050"
     CDS             complement(7033..7242)
                     /locus_tag="VF547_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262029.1"
                     /translation="MDETKKPKSKRVPNMDLLDRAMGEFQDYGRVVSVKCDECDGLIE
                     IVALGDSAWSMRCPCGKFNDTLRGI"
     gene            complement(7244..>8009)
                     /locus_tag="VF547_04055"
     CDS             complement(7244..>8009)
                     /locus_tag="VF547_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262030.1"
                     /translation="FATTAAGAAVVGAAGRAASAATRAVVPAAIRSGAASAGRAVATA
                     AARGAARAGSTAAGQLARRGVEAVGRRIVALEQASARRGLEASRSLFRPGSAGAQAAE
                     GNALRLAGSRGVRNTEPLTPSQQQQVYRHVEELGLDPSDFHISSRMSAYSDEFDAVFI
                     GPNAFPAPAASRVGGTVFERLSPRAVVAHEAGHMLTTRAGTSAAAGSVADEVGASLAA
                     ARLPGLNRLERYQLLRDAAERARNAGTTVRAILRGD"
BASE COUNT         1184 a         2783 c         2944 g         1098 t
ORIGIN      
        1 attgtagagc gaataggcgc cgcgcgggct gaaatcgacg tcctccaccg gatggtagac
       61 cgggtcgccg gcggtgttgt cgcggagcac gactccgttc accggggcgt agcggtcgtc
      121 gaaatcgagc tcgcccttga ggaactgggt cggccgcgag agcaatttgg gcactccgcc
      181 ctcgagcagc gcctcgcgga aggcgcagac catcgggtgc tggaagatcg agaagcgaag
      241 ccgccggaac ggatcgaccc acagcagcca ctcgaggaac agccgcacga aaggctggag
      301 gaaaaccggc gtgctcaaga tccattcgcg ccagatgtcg aggatgctgg acccggtgag
      361 cagccggccg tcggggaagg cggtgaccgg ttcgacgaag aagctgtggc gcttgtcggc
      421 gaagatccag ggcgcgcgag tccccgcgac gacatatttg tcgccgcggt cgaacgtcag
      481 cgaggtgccg aacagccagt tcagagcctg cagcgcctgg aggttgttga ggtcgaagct
      541 gttgaaatgg tgcggatagg tgatccgcac cttgtccggg gtcgcggcga ggaccggcgt
      601 gccgccgccg taggtgctgg tgccgaacgc gattagcggg ccgacctctt gagcctcgac
      661 gtcgcgcgcg gcccgcagct ccatgttatg gaaggtgacg gtgacaaagt aataatagtt
      721 ccgcgtcggc tccggatagc cgcggcagcc gaggaactcg tagcagtcgc cgtcgctcga
      781 atagaagcgg gcgtcgatat cctggaaata gagatgctcg tcctcggcga tccggcgcag
      841 gtcgaccggg gtccaggcga gccgctcgtg gtaatcgtcg cggtcgatcg gccgaaggaa
      901 ctgggccgac accttcttgg gcgaccattt gccgttgcga agctccgaca gcgcgacccc
      961 gcaggcgacc cgcggccgct cgaccgccgc gctgcccccg ccactcccgg ggatcgagat
     1021 cgtcgcctcg gtgctgggct tgacctcgcg gaactcgggc cagaccagga aggcgcggcg
     1081 atcgtacatg aacggcacca ccggggcgct cttcaggtcc agctcgatct tggaccaagc
     1141 cgtccagcgc tcggccggat ggtcgaaccg ccggtagaaa taggcgtgcg gctcctgccc
     1201 cgccgtccgg ccgacgacgt ggacggcgcc gcggcccgtc cccgctccgt cctgccagtg
     1261 cactccggtg acgtcgagat tggcgacctg gtcgagcttg tcgatatagt tgaggaaggc
     1321 gtcgcgggcg gaatcctcgg tcagctcggt ctgcagaagc tcgtcctcga gctccttgaa
     1381 ggcgtaggat ttgtcgcggc gaagctcggg ctccacccag ttttcgggga acaggaacac
     1441 tttgcgattg gcttcccaca ggcggtaggc gtgcatccat ttccaatggt cccaaagccc
     1501 atcggcggcc atggtcggcg agacctcggg ctcgaagccg aggaagcagc ggtcgacgaa
     1561 ggtctggatc gcgatcgtcg cctggacgat ccgcgacgtc atctggcagg cgcccatctt
     1621 ggggtcgatg agcaggaagg cgaagacgtc cgtcggcttg gcccagcgcc cggtcggcga
     1681 gggcggccgg ccgcccgttc caggctggaa ataggcgagc acgaagtccc tcaacccgtc
     1741 ccgccgcaac gccctcatct cgtcctggac ctcgcggccg gcctccgcat attgctggcc
     1801 gaggcggacg cgcatggcct ggcgggcggt cgccgcctcg gccgcggtcg gcgcggcgcg
     1861 ggcgagcttg agcgcgtcgg cggcgctcag gcgggtacgg cgcacgaccg cgagcgcgcg
     1921 ttcgagccga cgccaggccg cggcgttccg gaaatcgtcg ggataggcga ggccgaggcc
     1981 gacgcccgcg gccgcgagcg tcccgacctc gctcgccggc cagcccagcg attcctcggc
     2041 ggtggcgaac aggtcgagcg ttcccggccc cgggccgagg gtgacgctgg ccgggctggc
     2101 gaggatccgc gccatcaggt cgagcagcct gaccggcttg cgggccgtct tgcggtcgag
     2161 gagcagcagc gtccccagcc gcaccaggcg cgccagaggc gacagggccg ggggcgcggg
     2221 cggcggcccg ggagggcccg cataggcgag cggcaaggcc tggaagccga gcaggtcgct
     2281 gttcggagcg gcggcgaggg tctggacgag atcgatctcg cgcgcgccca tccgcagctg
     2341 ggcggcgatt cgcgccgcgc gatcgagcag gtcgaccgcc tggaattgcg gaagcagcgt
     2401 ggccggggtc accggccccg aggtggcgac gaagccgggc ccgtcggtca gcgtcgccat
     2461 cagcgtcgct ccggcgatcg agacccggtc gagcaccgcc gcaatggaat cgcggtcgag
     2521 ctcaacgacc gccgtcatcg cggtgacgac cgcctcgcgc gaggcggcgg cgcgggccct
     2581 gggcagcagc accgccagca tcgcgacgag attggcgcgg accttggcgt cggcggccac
     2641 ctcgtcgtcg gtcgtcggat cggtgaactg ccaggtggcg aaatcggcga gccgctgcgc
     2701 cgccggaacg ggcgtggcgc gcggctggtc gaacagcgaa gccaccgccg ccgcccagcc
     2761 cggatcggcc gacaccgcga gcaggtcggc gcgctccttc gccgtcatca cgccgtcgaa
     2821 ctgaagctgg cgccgagcgg cgacatatcg ggcccgcttc ttgagcgcgg ccggaagcgc
     2881 cgcgccggcc ggccaggccg cgagcggggc ctcgaagcgg gtgctgaccg tggcgacggc
     2941 gaggcgcggc tgctgctcga gagcgtcgat cgccggcgcc cagcggacgt cgccggccac
     3001 agggcccacc ttggccggat cgttgagcag cgatttgacg gcagggccga tcgcgccggt
     3061 atagctcagc acgagcttgc cgccgtcgac caccgccgag aagggcagct tggcgatgga
     3121 cgggtccagg acgggcagcg cggcgggaac gggggagatc tccgtccgcg cgacgaagcg
     3181 attctcgacc atttcggcga ccagcgccgc tcggcccgaa tcgggatcga ggggcgcgaa
     3241 cgcgcccggg agacggtcga taagggtctg gcggtcgctg tcgggcgccg gcggctgggt
     3301 ctgctccgcg atcgcctgga gggtcgcccg cagcccggca aggaccgcgc cggccgcctc
     3361 ggccgggagc gcgcccgcga cgtcgagacg cgcttcgagc aggtgggaga gcaaggcggc
     3421 gtcgatctcg agcgcccgcg ccgcggcgat cgtctccatc gcgccgagca gcgccgcggg
     3481 gctggcgaac ggatcgccgc cgaccagccg cgcgaacagg gtccattcgg cgatcgggat
     3541 gtcgagcgcc cgcgccaccc gggcgtgccg gtagagcagc gacaggttgg ccagcgaaag
     3601 cgcctgcccg gcgaacggga ggagctggtc gaggtcgctc gacgggatcc gcagcgcggc
     3661 caggatggcc gagcggtggc cggcgatctg tgccgccggg gccgccgccg tgacggccgg
     3721 aatctcgaag gccggatcga ccgggcggtg gaccagcggg tcgaggaaga gccgccgata
     3781 gagcgagggc gccggcggct cgtgcgcctc gacgaaccga tcctcggtcg gaatcgcgcc
     3841 gaacagggcg accagctcct cgaccgggag cttgcccagc tcggagcgga cccgcatcgc
     3901 gtcggcgatc gcgatcaggt cctgctggtt gcccagcgcc ccgccggcgc cgaagcgtcg
     3961 gatcagcagg tcggtgtcgg ccgcctccag gccgagcgcc ctggcgaggc ggaggaatcg
     4021 gtgcagccgg tccaggctcg gcgcgtcgag cgtgatcagc cgtttctgag cgaggtcgca
     4081 gctgtcgtcg agatgctcga tggcgagcgc gccgttgaga ttggcatagg gcagcgacaa
     4141 cagccgcagc aggtcggcgt agctgaggcc ggtcacggcg aggaaggtcg ggacctccct
     4201 gtatttggcc acggcctggg cgacgctgcc gacgccccaa tgggccgagg gattgggggc
     4261 cggcgtgacc aggaccgaag cgagcgccga gctgattccg atcgttgcgg cggcgagttc
     4321 gaacggcgtc gggttgttgg gagcggccgg tccgcgaagg gcgcgcatca ggtcgtcccg
     4381 ccggatcccg agcttggcca gcgagagcct ggtctcggcg ccgtccaggt cgagcggcag
     4441 cgtccaggga aagctcttcg cgttgagggc ggccgtcgca tggggattcg aatattcggg
     4501 catcagctcg cggtcctgcg gccggccgtg ggtgttgcgc tgcaccagca cattgggacc
     4561 ggccccgctg tccaggatcc ggaagccgct ttcgccgtcg cgaagcaccc agctggagcc
     4621 cgccttgcgc acgcgcgcgg cggcggacag ggcgatcggc ggggcgaagg ccgaaaaggc
     4681 ggcgacgacc gccgccggga cggcgtggtc ggcgctgtcg gaaggcagcg tcgtgcgctg
     4741 cgcggcggtg agcgttccca ccgaatgcgg ggcgacgaga tcctccatcg cctcgatcgc
     4801 caggtccaca tagggaagcg gggtgaacgc gttcgggcaa ttgaggtcga tccaggccag
     4861 gtccggccgg cgtgcgagca ggacgtcctt ggcgctgcgg ccgtcgccga gcttgcggtt
     4921 ggagaggaag aggagcaggt cggccagata ggcggccggg ctcagcaccg agctgcagtc
     4981 ggcgcagacg cagtcgttgc ccatcgcgaa cagcgagccg agctcgggca ccgtctgggc
     5041 ctcgtcggcg aggccgctca tcaccggcac gtcgaggacc tgggtctcgg acgcgacgcc
     5101 gaccagcagc tgaaggctcg ccgcggcggt cgcgcccgcc cgttcgaaga cgagctgcgc
     5161 ggcccgttcg ctgaggccgg cttcgtcggt gaggaggcgc accgcctggt cctcgccgag
     5221 ccggtagacg gcctgggccg atccgaggcc atgacgcgca agcgtggcga ccgccgaata
     5281 atcggtgctg atcttgtaga gccgctgcag cggcttgatc gactcgccca tctcgcggag
     5341 cgactcggcg cgcgtccccc gccgcggctg tcggccgccg cgcgccgggc cgcgcttgcc
     5401 ggcgctccgg gccagatatc ggtcgatcga gatgttggcc aggtcgagct ccggctcgtc
     5461 gccgatcagg gccgcgaggt cgcgcagcgg ctgcggggcc ttcctgtccc gccccgcgtc
     5521 cccaaggaac gcctgggtcg gatagagcga gcggaagcgg gtcgcgagga gggctgcgta
     5581 atcggccttc ttgccgcccg cctcggcgac gaagggcggc ggctcggcct tggcgtcctt
     5641 gatcagccgc gaccattgcc gggcgtccag ccgcgccagc aaacggacgg aatcggccga
     5701 ctcgaccttg tcggcgagac cgagcaccag cgcctggtcg ccgaacgcgg cggtgttgag
     5761 agcgaccagg ccctcgacct tgcggacgat ccggtcggac acctgcccgt ccttccgcag
     5821 gctccggacg aggctggcgc cgtcgagcgc cttgtcgagc tggccctcga tcgcggcggc
     5881 gtcggtgccg agcgcctgcc tgaggagggt cccgggcagg gcggcgccgg gagcgctgcc
     5941 gccctcgcgc ccggcaagat cggcgaaggc gcggacgaac cgctcgatct ccgccttcgt
     6001 cgcggcgacg acaccggtct cgaccgaggt gtccagcgca agccggaccg cccgcatcga
     6061 cggctccccg aagcgagccc gcagcgcccg ggcccgtgcg tccagatccc ggatcgcgcc
     6121 gggctcggtg ccggccggct ccggccacac gccggagagg accagcgcga acagcaggcg
     6181 cgggtcgagg ccggtctggg cggtcagccg cgccgccacc gcgagggccg cgacctcgaa
     6241 ggcggcgaag ccggtctcgc cggccaggaa gctgacgtcg cggaaccgct cggtctcgtc
     6301 gatctcggcg agctcgaccc cctgcccctc ggcgagcggc tcgatcgcct cgatcagccg
     6361 ctggtagagc gaaggcccgc gcatctcgag ccggatgtcg tggaccaggc gcgccggcgc
     6421 gttgaacagc acctcgctct cgtagagcgg ctcgtcggcg cccggacgga gaagctggac
     6481 gacgatgtcg gcatagccct tctcggctcg agcgaactgg ccggcttcgt aggcgaccgc
     6541 atagtcgccg gagcggtcgg tcgaggcggt gccgagcagc tgacggtcgc gaaggtcgcg
     6601 gtcgaacacc cgcacctgaa ggccggcgaa ggcgactccg tcggagccgc gcaccctgcc
     6661 ctcgacccgc agcagctcgg ccggcccgcc ggccggaccg gcgatcctgc cggcgccggc
     6721 aagccggcgg ttgatctccg ccgccgccgc cgcgctgagg acgacccgct cgtcgatcgc
     6781 aaggcccgcc gagcgctcga tcgaccggac cgcctgcagg gtggaccgac cggcggacgc
     6841 ggattccctt tcctctccgg caatccggaa gccgagcgcg tcgagcgcgc actggagctc
     6901 gcgcacccgg tcggaatcct cgcgatcgat gctgttcacc atcgagatcg actcggtcat
     6961 gctagcctcc gtgccttcga actaagcctc gtcccggttc caccgtcttt caggcgcctg
     7021 tgccttcgcc gctcaaattc cgcgcagcgt gtcgttgaac ttcccgcagg gacagcgcat
     7081 cgaccaggcg ctgtcgccca gcgcgacgat ctcgatcagc ccgtcgcatt cgtcgcactt
     7141 gacgctgacc acccgtccat aatcctggaa ctcgcccatc gcccgatcga ggaggtccat
     7201 gttcggcacc ctcttgctct tgggcttttt ggtctcgtcc atttcaatct cctcgcagga
     7261 tcgcccgaac cgtcgtgccc gcattcctcg cccgctcggc cgcgtcgcga agcagctgat
     7321 atctctccag gcggttgagg cccggcagac gggccgccgc gaggcttgcg ccgacctcgt
     7381 cggcgacgga acccgcggca gcactggttc cggcgcgcgt cgtcagcatg tgtccggcct
     7441 cgtgcgccac caccgccctc gggctcagcc gctcgaacac cgtccctccg accctgctcg
     7501 cggccggtgc cgggaaggcg ttgggaccga tgaagaccgc gtcgaactcg tccgaataag
     7561 cggacatccg ggagctgata tggaagtccg acgggtcgag gccgagctcc tcgacatgcc
     7621 ggtagacctg ctgctgctgg cttggcgtca gcggctccgt gttcctgacg ccccgggagc
     7681 cggcgagtcg aagcgcgttg ccctccgccg cttgcgcgcc cgccgatccg ggtcggaaca
     7741 ggcttcgcga tgcctcgagt ccgcgccggg cggaagcctg ctcgagcgcc acgattcgac
     7801 gcccgaccgc ttccacgcct cgccgggcga gctgcccggc cgcggtcgag ccggcccggg
     7861 ccgcgccgcg cgccgccgcc gtcgccaccg ctcggccggc gctcgccgcc ccggagcgaa
     7921 tcgccgccgg gaccaccgct cgggtcgccg ccgaggccgc ccgtcccgcc gctccgacca
     7981 cggccgcccc ggccgcggtg gtcgcgaac
//
LOCUS       DASVQZ010000146         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_800673, whole genome shotgun sequence.
ACCESSION   DASVQZ010000146 DASVQZ010000000
VERSION     DASVQZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800673"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VF547_04060"
     CDS             complement(<1..445)
                     /locus_tag="VF547_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8262031.1"
                     /translation="MRSAALALALAGLAAAAPNAAAAPARKAAPTAVDWGRTVVATPE
                     GGFRMGNPNARVKLVEYGSLTCGHCAAFSRQGMAPLVATYVKSGRVSYEYRNFILNGL
                     DVAASLVARCGGPARFFPVADKFYATQPQWMGRVSALTAAQKAQLN"
     gene            complement(442..1185)
                     /locus_tag="VF547_04065"
     CDS             complement(442..1185)
                     /locus_tag="VF547_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8262032.1"
                     /translation="MKSMFMAGAAALALLLAGCGDGAGGNGQAAAAGNAPLKQIPAPN
                     GDWTQTVVKTPEGGFRMGNPDAPVKLVEYGSLSCPHCAEFEEQGAPELRDTYVKSGQV
                     SWEFRTYLLFGPDAGISALLHCQGESAFFPFVEQLYADQRNWGGRLQSLPPEQLQQIQ
                     SLPPREAAPAMIRAAGIDEFFRQRGMPASRIQSCLADSRVLDQVADITKRGNEEGVNG
                     TPSFFINGKLVERAASWAALEPELRAAIP"
     gene            complement(1182..1751)
                     /locus_tag="VF547_04070"
     CDS             complement(1182..1751)
                     /locus_tag="VF547_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX8262033.1"
                     /translation="MAKSGGTSKRQIEAPRANRARAVSDIVPDVGRAAFRRFGFVQAS
                     IVSRWREIVGDRYAAVSSPESIRFPPGKRSDGVLNLVVEGAHAPMMQHVAPAIVERVN
                     RFFGYQAVGRVAFRQGLVQVARATSRAAPPSLRPIPAELGDSLREVADPELRACLESL
                     ARGVAANEAALGPAVAIPVIGRIGGETKR"
     gene            1778..>2082
                     /locus_tag="VF547_04075"
     CDS             1778..>2082
                     /locus_tag="VF547_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX8262034.1"
                     /translation="MSVDASEALLRWFAVDKRRLPWRPEGGARPDPYRTWLSEIMLQQ
                     TTCAHAAPYFDRFTRRWPTVGALAAAPEAEVMAAWAGLGYYARARNLIACARVVQSR"
BASE COUNT          301 a          739 c          737 g          305 t
ORIGIN      
        1 cgttgagctg ggccttctgc gcggcggtca gcgccgaaac gcgccccatc cattgcggct
       61 gcgtggcata gaatttgtcg gccaccggga agaagcgcgc cgggccaccg caacgggcga
      121 ccaggctggc ggcgacgtcg aggccgttga ggatgaagtt gcgatattcg tagctgaccc
      181 ggccgctctt cacataggtg gcgacgagcg gcgccatgcc ctgcctcgag aaggccgcgc
      241 aatgcccgca ggtcagcgag ccatattcga ccagcttcac ccgggcgttg ggattgccca
      301 tccggaagcc gccctcgggc gtggcgacga cggtccgccc ccagtcgacc gccgtcggcg
      361 ccgccttgcg cgccggagcc gcggcggcgt tcggcgcggc cgcggccagg ccggcgagcg
      421 ccagggccag cgccgcgctc ctcacgggat cgccgccctc agctcgggct cgagcgcggc
      481 ccagctcgcc gcacgctcga ccagcttgcc gttgatgaag aaggaggggg ttccgttgac
      541 gccctcctcg ttgccgcgct tggtgatgtc ggcgacctgg tcgagcacgc ggctgtcggc
      601 gaggcaggac tggatgcgcg aggccggcat tccgcgctgg cggaagaatt cgtcgatccc
      661 ggcggcgcgg atcatcgccg gagcggcctc gcgcggcggg agggactgga tctgctgcag
      721 ctgctccggc ggcagcgact gcagtcggcc gccccagttg cgctggtcgg cgtagagctg
      781 ctcgacgaac gggaagaagg cgctctcccc ctggcaatgg agaagggccg agatgccggc
      841 gtcgggcccg aacagcagat aggtgcggaa ttcccagctc acctggccgc tcttgacgta
      901 ggtgtcgcgc agctcggggg cgccctgctc ctcgaactcg gcgcaatgcg ggcagctcag
      961 cgagccatat tcgaccagct tcaccggggc gtcgggattg cccatgcgga agccgccttc
     1021 cggcgtcttc accaccgtct gggtccagtc gccgttcggc gccgggatct gcttgagcgg
     1081 cgcgttgccg gccgcggccg cctggccgtt gccgcccgcg ccgtcgccgc agccggcgag
     1141 aagaagggcc agggcggccg caccggccat gaacatcgac ttcatctctt cgtctctcca
     1201 ccaattctgc cgatcaccgg gatggccacc gccggaccca gcgccgcctc gttcgccgca
     1261 acgccccggg ccagcgattc gaggcaggcg cgaagctcag ggtccgcaac ctcgcgcagc
     1321 gaatcgccga gctcggccgg gatcggccga agcgacggcg gcgcggcgcg gctcgtcgcc
     1381 cgggcgacct gaaccaggcc ttgcctgaag gcgacgcggc ccaccgcctg atagccgaag
     1441 aagcggttga cccgctcgac gatcgccggg gccacatgct gcatcatcgg cgcatgcgcg
     1501 ccctccacga ccaggttgag gacgccgtcg gagcgcttgc ccggggggaa gcggatcgat
     1561 tccggggagg agacggccgc gtagcggtcg ccgacgatct cgcgccagcg gctgacgatc
     1621 gacgcctgga cgaagccgaa gcggcggaac gcggcgcggc ccacatcggg gacgatgtcg
     1681 gacaccgcgc gggcccgatt ggcccgcggc gcctcgatct gccttttcga cgtcccgccc
     1741 gatttcgcca tccggccgat catggcatag gcgaagcatg tccgtcgatg cttcagaggc
     1801 cctgttgcgg tggtttgctg tggataagcg gcggttgccc tggcgccccg aaggcggggc
     1861 ccgaccggat ccttaccgga catggctttc cgagataatg ctccagcaga ccacctgcgc
     1921 ccacgccgcg ccctatttcg accgcttcac ccggcgctgg ccgacggtcg gagcgctcgc
     1981 cgcggccccg gaggcggagg tgatggcggc ctgggccggg ctcggctatt acgcccgcgc
     2041 ccgcaatctg atcgcctgcg cacgcgtcgt ccaatcccgg tt
//
LOCUS       DASVQZ010000147         4217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_510895, whole genome shotgun sequence.
ACCESSION   DASVQZ010000147 DASVQZ010000000
VERSION     DASVQZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510895"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VF547_04080"
     CDS             <1..471
                     /locus_tag="VF547_04080"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8262035.1"
                     /translation="NNWAHVGLIALILPNARIVDARRHPLACCFSNFKQHFARGQGFS
                     YDLAELGRYYRAYVELMRHFDDVLPGRVHRVSYERMVEDPEGEVRRLLDYLGLPFEDS
                     CLRFYENQRAVRTASSEQVRQPINREGLDQWRAFEPWLAPLKDALGPVLDAYPE"
     gene            608..3043
                     /locus_tag="VF547_04085"
     CDS             608..3043
                     /locus_tag="VF547_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8262036.1"
                     /translation="MRLAPSLKLAAFLLASVAFPAAVLAQEEADPPATGEGSGAGDLA
                     DTGRGQSDDADIVITAQKREENLQDVPIAVTAFGTEKLDELQVNEFADYARQVPSISF
                     QSAGPGFSNVYFRGVASGENANHSASLPSVGTYLDEQPITTITGALDIHVFDIARVEA
                     LAGPQGTLYGASSQAGTVRIITNKPDTSGAYGEANVELNNVAHGEWGYSGEAFFNAPV
                     SSRAALRVVGWYRKDAGYIDNVPGSLTFPTSGITFDNAPFVEEDYNDVETYGARAALK
                     IDLDDNWTILPQVMAQKQVSDGTFAQESGLGQLQVQQFNREHFEDKWYQAALTVSGKI
                     GNFDVTYAGAYMQRQIDGASDYVDYAYFYDALAGYGAYFYDNNGDFVNPNQFILSDDS
                     FTKQSHELRLASPADKPIRFIGGLFYQRQTHSIEQNYIIEDIADTLTVPGTESNIWLT
                     QQMRVDRDYAVFGEVSWDITPQLTLTGGGRLYRFKNSLEGFFGFSSGYSSKTGVAACF
                     GPPQVAGSPCTNLDKTTAATDFIHRLNLTWKPNDDILLYGTWSRGFRPGGVNRRGTLP
                     PYPPDFIDNYEFGWKTSWADNRLRFNGALYQLNWSDIQLSFLGANGLTEIRSAGNARI
                     RGAEFDLFYRPVRGLTFSLGGSFNDAKITDDFCKIANEDFNCALPGSGGEANALLAPA
                     GTRLPITARFKANALARYEFKLGALDAHVQGNLIHEGSRTSDLRLAEREIVGRLGAYQ
                     TVDLSTGLTNGRWSAELFVKNLFDVNGATGNTIQCTESVCGDPDGLTAIGPKIYTYVI
                     RPRMIGLRLGTKF"
     gene            complement(3217..3609)
                     /locus_tag="VF547_04090"
     CDS             complement(3217..3609)
                     /locus_tag="VF547_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8262037.1"
                     /translation="MALTMWGEARSGGEDAMRAVGHVIDNRRRSGAHGVYATETVSEA
                     WQFSCWNKNDPNHRAMLNVDSLKPGGEDQLMWLAAKRLAAEILSGASADPTGGALFYH
                     TAAVAPRWSAGLAPVRVIGGHLFFRTAR"
BASE COUNT          758 a         1533 c         1254 g          672 t
ORIGIN      
        1 aacaactggg cgcatgtcgg gctcatcgcc ctcatccttc ccaatgcgag gatcgtcgac
       61 gcgcggcggc acccgctcgc ctgctgcttc tccaacttca agcagcattt cgcccgcggc
      121 cagggcttca gctacgacct cgccgagctc ggccgctatt accgcgccta tgtcgagctg
      181 atgcggcatt tcgacgacgt gctgccgggg cgggtccacc gggtgagcta cgaacggatg
      241 gtcgaggatc ccgaggggga ggtgcggcgc ctgctcgact atctcgggct tccgttcgag
      301 gactcctgcc tgcgcttcta cgagaaccag cgggcggtgc ggacggcgag ctcggagcag
      361 gtgaggcagc cgatcaatcg cgaaggcctc gaccagtggc gcgccttcga gccgtggctg
      421 gcgccgctga aggatgcgct gggcccggtg cttgacgcct atccggaata atccccctcc
      481 ctgcagaggc gtgacctcga aaccacagcg ctgcgctaga accgtttcgc ttacagaata
      541 agtttgaccg cttcccccaa agcaagacag attcggcggc gacgcaaact ccgggggatt
      601 tgccgaaatg cgcctcgcac ccagcctgaa gctcgcggcc ttcctgctcg cctcggttgc
      661 ctttcccgcg gccgtgctcg cacaggagga ggccgatcct cccgcaaccg gcgagggaag
      721 cggcgccggc gacctggccg acaccggccg cggtcagtcc gacgacgccg acatcgtcat
      781 caccgcccag aagcgcgagg agaacctcca ggacgtgccg atcgcggtca ccgccttcgg
      841 caccgagaag ctggatgagc tgcaggtcaa cgaattcgcc gactatgccc gccaggtgcc
      901 ctccatctcg ttccagagcg ccggccccgg cttctccaac gtctatttcc gcggcgtcgc
      961 cagcggcgag aacgccaacc actccgcgtc cctgccctcg gtcggaacct atctcgacga
     1021 gcagccgatc acgaccatca ccggcgcgct cgacatccac gtcttcgaca tcgcccgcgt
     1081 ggaggcgctc gccggccccc agggcacgct gtacggagcc tcgagccagg ccggaacggt
     1141 ccggatcatc accaacaagc ccgacacgag cggcgcctat ggcgaggcca atgtcgagct
     1201 caacaacgtc gcccacggcg agtggggtta ttccggcgag gccttcttca acgctccggt
     1261 ctcgagccgg gcggcgcttc gggtcgtcgg ctggtaccgc aaggacgcgg gctatatcga
     1321 caacgtcccg ggcagcctga ccttcccgac ctcaggcatc accttcgaca acgccccctt
     1381 cgtcgaggag gattataacg acgtcgagac gtatggcgcc cgcgccgcgc tcaagatcga
     1441 cctcgacgac aattggacga tccttccgca ggtgatggcc cagaagcagg tgagcgacgg
     1501 caccttcgcc caggaatccg gcctcggcca gctccaggtc cagcagttca accgcgagca
     1561 tttcgaggac aaatggtatc aggcggctct caccgtctcc ggcaagatcg gcaacttcga
     1621 cgtcacctat gccggcgcct acatgcagcg ccagatcgac ggtgcgtccg actatgtcga
     1681 ctacgcctat ttctacgacg cgctggccgg ctacggcgcc tatttctacg acaacaacgg
     1741 cgacttcgtt aatcccaacc agttcatcct ttcggacgac agcttcacca agcagagcca
     1801 cgagctgcgg ctcgcctcgc ccgcagacaa gccgatccgc ttcatcggcg gcctgttcta
     1861 ccagcgtcag acgcacagca tcgagcagaa ctacatcatc gaggacatcg ccgacacgct
     1921 caccgtcccc ggcacggaga gcaacatctg gctaacccag cagatgcgcg tcgaccgcga
     1981 ctatgccgtc ttcggcgagg tcagctggga catcactccg cagctgaccc tcaccggcgg
     2041 cggccggctc taccggttca agaactcgct cgagggcttc ttcgggttca gctcgggcta
     2101 cagcagcaag accggagtcg cggcctgctt tggcccgccg caggtcgccg gctcgccctg
     2161 caccaacctc gacaagacga ccgcggcgac cgacttcatc caccgcctca atctcacctg
     2221 gaagccgaac gacgacatat tgctctacgg cacctggtcg cgcggcttcc ggccgggcgg
     2281 agtcaaccgg cgcgggaccc tgccgcccta cccgcccgat ttcatcgaca attacgaatt
     2341 cggctggaag acgagctggg ccgacaaccg gctgcgcttc aacggcgccc tctaccagct
     2401 caactggtcg gacatccagc tctccttcct cggcgccaac ggcctcaccg agatcaggag
     2461 cgccggcaac gcccgaatcc gcggcgccga gttcgacctc ttctaccgtc ccgtgcgcgg
     2521 cctcaccttc agcctcggcg gctcgttcaa cgacgccaag atcaccgacg atttctgcaa
     2581 gatcgccaat gaggatttca attgcgcgct gcccggctcc ggcggcgagg ccaacgcgct
     2641 cctggcgccg gccgggacgc ggcttccgat caccgcgcgc ttcaaggcca atgcgctcgc
     2701 ccgctacgag ttcaagctcg gagcgctcga cgcccatgtc cagggcaatc tcatccacga
     2761 aggctcgcgc accagcgacc tcaggctcgc cgagcgcgaa atcgtcgggc ggctcggagc
     2821 ctatcagacg gtcgacctct cgacgggcct caccaacggc cgctggtcgg cggaattgtt
     2881 cgtcaagaac ctgttcgacg tgaacggcgc caccggcaac accatccagt gcaccgagag
     2941 cgtctgcggc gaccccgacg gcctcaccgc gatcggcccc aagatctaca cctacgtcat
     3001 ccggccgagg atgatcggcc tgaggctcgg cacgaagttc tgatctggcc ctccccctcg
     3061 ccagatggcg agcgggacca tgcgaagcat ggtggaggcc tgaccggccg aaatggctgc
     3121 ggatccagcg ctcgaccgcg aagcccgcta gatcattcct ccaccccctt cggtggcccc
     3181 cttccccatc gcatccgcga cgggcaggca gagggttcag cgcgccgtcc ggaagaagag
     3241 atggccgccg atgaccctca ccggggcgag acccgccgac cagcgcggag cgaccgcggc
     3301 ggtgtggtag aagagcgctc cgccggtcgg atcggccgag gctccggaga ggatctccgc
     3361 cgccagccgc ttcgccgcca gccacatcag ctggtcctcg ccgccgggct tcagcgaatc
     3421 gacgttgagc atcgcccggt gattgggatc gttcttgttc cagcagctga actgccaggc
     3481 ctcgctgacc gtctcggtcg cgtagacgcc gtgcgcgccg ctgcggcggc ggttgtcgat
     3541 gacgtggccg acggcgcgca tcgcgtcctc gccgccgctc ctcgcctcgc cccacatggt
     3601 cagtgccatc caatgcgcgt cgacgacgtc gatctcctcc gccggaacga tctccgtggc
     3661 gggcgcggaa gcttcgggcc cggcggccgg acgctcggaa tgcggctgaa gcggccgggt
     3721 ccactccccc cagtccgcgc gctcctgctt gacgggcgca gcaggcgcga ccgggccatc
     3781 gccctcagcc cgggcggccg caatcgcaac aataacagcg gcaatcgccg cccccgcagc
     3841 ccatttcata atgaaaataa tgccttaatt cgaaggcttt ggccagccct ttaagtctgc
     3901 ctgctcccag acggaaccgg accgcctaat tcaggcctga ccgctcgggc ccgaagcgct
     3961 gaagatactt cctcacctcg ccgaccggct cgggctccca tacccctcgc cggcgtagct
     4021 tcaccacggt gaagccgtcc cacacagccg ggcgggcgcc gagtgattgc gaagttcctc
     4081 gaggggcgga tcgccttgat gaagaaggac gacggccgcc cagctggtaa cgattgcaac
     4141 cggaccttca ttccgatcac accgccaaat agtgacagta tactttcacc gccagggtcc
     4201 cctcgcgcgc gaggcgg
//
LOCUS       DASVQZ010000148         5028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_226642, whole genome shotgun sequence.
ACCESSION   DASVQZ010000148 DASVQZ010000000
VERSION     DASVQZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226642"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VF547_04095"
     CDS             <1..138
                     /locus_tag="VF547_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8262038.1"
                     /translation="VERFFCKLKQFRRVATRFDKLARNFLAAVLLASTRLWLRTYEST
                     P"
     gene            complement(135..1640)
                     /locus_tag="VF547_04100"
     CDS             complement(135..1640)
                     /locus_tag="VF547_04100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8262039.1"
                     /translation="MYQYSQIRPDAGGGAAALAFESRPRPRSFGSRAAPRPRDFDLVV
                     DLGARIGSRTWFRGAFTCTALCCTALWLTPGFDPIPAASAAPLSDAHFAQARSLAIAP
                     LALGADTGRRMAPTDAVEPLLDTPERPTVELNATIGEGDGFVRALERAGVAPKEADEV
                     ARLVAGAVPLDQLKPGTTMAVTLGRRPDRTVARPLTALALRARFDLKLSLKRTGGKLA
                     LTRTPISVDSTPLRIQGFVGSSLYRSARAAGAPARAVEAYIRAVASQTSIGGLAPDDR
                     FDIILEHRRAATGEAETGRLLYAGLDRIAGKDLRLMQWEQGGRAEWFEASGVGRESGM
                     LQRPVPGNVSSNFGLRLHPILGYTRMHKGVDFRAGYGTPILAATDGRVIAAGRAGGYG
                     NQVRIAHSGGLVTTYSHMSRIVAQAGGAVRQGQVIGYVGSTGLATGPHLHYELYKDGV
                     PINPTSVRFVSRSQLDGASLAAFRSRLKSLLAVKPGAARMADSAARKKPRA"
     gene            complement(2028..2231)
                     /locus_tag="VF547_04105"
                     /pseudo
     CDS             complement(2028..2231)
                     /locus_tag="VF547_04105"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
     gene            2418..4934
                     /locus_tag="VF547_04110"
     CDS             2418..4934
                     /locus_tag="VF547_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX8262040.1"
                     /translation="MSEFARAPLAAILGPTNTGKTHLAVERMAAHSSGMIGFPLRLLA
                     REVYDRVVAMKGANRVALITGEEKIVPKDSRWFLCTAESMPMEKDFAFVALDEAQLGA
                     DPERGHVFTDRMLHARGREETMILGSETLAPMVRALLPKAEIIGRPRFSTLSYAGPSK
                     LSRLPPRSAIVAFSAEQVYAVAEMLRRMRGGAAVVMGALSPRTRNAQVAMYQAGEVDY
                     LVATDAIGMGLNMDVTHVAFAGLSKFDGHRRRRLTPAEMAQIAGRAGRHQRDGTFGVL
                     TLEGELSAEFRPEELEAIEEHRFAPLDHLYWREGDPDFRSVEALVRSLERRPDEPGLR
                     AAPEAIDLAVLKRLAEDPDVAARARGAEAVRRLWAACGLPDFRKTGPEPHARLVGRLY
                     LHLSEGDGRIPHGWFAAQIAQLDNVQGDIDALADRLAGVRTWAYIAHRADWLADPAVM
                     AERTREVEERLSDALHERLTQRFVDRRTSVLLKDIGRKGVGEFPVIVDEQGEVTVGSF
                     PIGRMEGFGFEVDPTARHSDRKMLLATAERRLGGEYERRAAALAADSDDHFTLRTQAG
                     QAVAVLWRGHEVARLGPGKNLLSPRVLVDRRLDRLSERGRATVVARLDLWVRHQVERA
                     LGSLRQAGLAAQDPRAPAAVRSILAMLVDEGGIIAREAVAKPLAELDRDQRRTVTRLR
                     TRIGALDLYMPDVLKPEARRWRTALRAAAAGEPMPELPPESAVVLPAPADGEQRRLTR
                     LGFRPLGPQMLRVDLVERLARHAHEARTGKPQPVVDEALATSLGLQPQAVAKLMRDLG
                     FRPADGAQAWVWRGRSPRRDEPRRPASGNAFAALAGLKRG"
     gene            4939..>5028
                     /locus_tag="VF547_04115"
     CDS             4939..>5028
                     /locus_tag="VF547_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX8262041.1"
                     /translation="MRLDKFLWFARIVKTRALAQQLAEQGRIRI"
BASE COUNT          704 a         1753 c         1871 g          700 t
ORIGIN      
        1 gtcgagcggt tcttctgcaa gctcaagcag ttccgacgcg ttgccacccg cttcgacaag
       61 ctcgcccgca acttcctcgc tgcagtcttg ctcgcctcaa cccgactttg gctcagaact
      121 tatgagtcca cgccctaggc gcggggcttc ttcctggcgg cggaatccgc catccgcgcg
      181 gcgccgggct tgaccgcgag caggctcttc agccgcgagc ggaaggcggc gaggctggcg
      241 ccgtcgagct gggagcggct gacgaagcgg accgaggtcg ggttgatcgg aacgccgtcc
      301 ttgtagagct cgtaatggag gtgcgggccg gtggcgaggc cggtcgagcc gacatagccg
      361 atgacctggc cctggcggac ggcgccgccg gcctgggcga cgatccggct catgtgcgaa
      421 taggtggtga cgagcccacc ggaatgcgcg atccggacct ggttgccgta gccgccggcg
      481 cggccggccg cgatcaccct gccgtcggtc gcggcgagga tcggggtgcc gtagccggcg
      541 cggaaatcga cccccttgtg catgcgcgtg tagcccagga tcgggtggag cctgaggccg
      601 aagttggacg agacgttgcc gggcacgggc cgctgcagca tgccgctctc gcggccgact
      661 ccggaggcct cgaaccattc ggcgcggccg ccctgctccc attgcatgag ccgaaggtcc
      721 ttgccggcga tccggtcgag cccggcgtag agcagacgtc cggtctcggc ctcgccggtg
      781 gcggcgcggc gatgctcgag gatgatgtcg aagcggtcgt ccggcgccag gccgccgatg
      841 ctggtctggc ttgcgaccgc ccggatatag gcctcgacgg cgcgggccgg agcgccggcg
      901 gcgcgggccg agcgatagag gctggagccg acgaagccct ggattcgaag cggggtcgag
      961 tcgaccgaga tcggggtccg ggtcaaggcg agcttgccgc cggtgcgctt cagcgacagc
     1021 ttgaggtcga agcgcgcgcg cagcgccagc gcggtcagcg gccgcgcgac ggtgcggtcc
     1081 gggcggcggc cgagcgtgac cgccatggtc gtgccgggct tgagctggtc gagcgggacg
     1141 gcgccggcga cgagccgggc gacctcgtcc gcctccttcg gggcgactcc ggcgcgctcg
     1201 agggcgcgga cgaagccgtc gccttcgccg atcgtggcgt tgagctcgac cgtcggccgc
     1261 tcgggcgtat cgagcagggg ctcgaccgcg tcggtcggcg ccatccggcg gccggtgtcg
     1321 gcgcccagcg cgagcggcgc gatggcgagc gagcgcgcct gggcgaaatg ggcgtccgaa
     1381 agcggcgccg cggacgccgc cggaatgggg tcgaagccgg gcgtcagcca caaggcggtg
     1441 cagcacaggg cggtgcaggt gaaggcgccc cggaaccagg tgcgcgagcc gatccgggcg
     1501 ccgagatcca cgacgaggtc gaagtcgcgc ggccgcggcg cggcgcggct gccgaagctt
     1561 cgcggccgcg gccgggactc gaaggcgagc gcggcggctc cgccgccggc atccggcctg
     1621 atctggctat actggtacaa ggcggaaaaa gcccccgcga cccgtgaaac accccccggc
     1681 cgcggatcgt ggccaggcga caaggactgt ggcgaatcac ggttaaagtc cagtaaactt
     1741 ctcctgcccg cgcaaagggt tgcgacgaac cgtgccgggg gagcgggtga gccgttcacg
     1801 atcgccggaa ccagtcctcc ccgtgcgcga agcgcatggg gagggggacc gcgcgcagcg
     1861 cggtggaggg gcgcccccgt cagcgggatc cggcgagcga caggatcaga ttcacggcgg
     1921 gctcgatatc gccatagagg tgacgggccg gaatgcggca gatgcgaacc ccgctcatcc
     1981 agccacgcgt cgcgtgctgc atcccgtgcc ggaacctcgc ccatctcatg gacgaggccg
     2041 tcgatctcga tcgccagctt cgaggacggg caatagaagt caagcacgta cggcccaacc
     2101 ggatgctgcc gcctgaattt cagcccgttc ggccgtgttc gcagcacctg ccagagacgg
     2161 gcttccggtc gagtcatcat ccttcgaagc gctcgcgctt tggccaccgt cgctttcgtt
     2221 gtgcctcgca tcgcccctcc accgcacgtc gtgcggtccc cctccccatc cgcttcgcgg
     2281 acggggagga cggttttaca ccgcgcctgt ccttgccata aatgtcagca cttccggcag
     2341 gttttttcct ggggtttcgt tttacgatta gaacgcaagt cattgcactc ggcgcgggaa
     2401 cgaccgatct acgggtcatg agcgaattcg cccgggcgcc gctggccgcc atcctggggc
     2461 cgaccaatac cggcaagacc cacctcgccg tcgagcggat ggcggcccat tcgagcggca
     2521 tgatcggctt ccccctgcgg ctgctggcgc gcgaggtcta cgaccgggtg gtcgcgatga
     2581 agggcgccaa ccgggtcgcc ctgatcaccg gcgaggagaa gatcgtcccg aaggactcgc
     2641 gctggttcct gtgcacggcc gaatcgatgc cgatggagaa ggacttcgcc ttcgtcgccc
     2701 tcgacgaggc gcagctcggc gccgatccgg agcgcggcca cgtcttcacc gaccggatgc
     2761 tccacgcccg cggccgggag gagacgatga tcctcggctc ggagacgctg gcgccgatgg
     2821 tccgggcctt gctgcccaag gccgagatca tcggccgacc gcgcttctcg accctctcct
     2881 atgcggggcc gtccaagctg tcgcggctgc cgccgagatc ggcgatcgtg gccttctcgg
     2941 ccgagcaggt ctacgccgtc gccgagatgc tccggcggat gcgcggcggc gcggcggtgg
     3001 tgatgggggc gctctcgccg cgcacccgca acgcccaggt ggcgatgtac caggccggcg
     3061 aggtcgacta tctcgtcgcc accgacgcga tcggaatggg gctgaacatg gacgtcaccc
     3121 atgtcgcctt cgccggcctc tccaagttcg acggccaccg gcggcggcgg ctgaccccgg
     3181 ccgagatggc gcagatcgcc ggccgcgccg ggcgccacca gcgcgacggc acgttcggcg
     3241 tgctgacgct cgagggcgag ctttccgccg agttccggcc cgaggaactg gaggcgatcg
     3301 aggagcaccg cttcgcgccg ctcgatcatc tctactggcg cgagggcgac cccgatttcc
     3361 ggagcgtcga ggcgctggtc cgctcgctcg agcggcggcc ggacgagccc ggcctgcgcg
     3421 ccgcgccgga ggcgatcgac ctcgccgtgc tcaagcgtct ggccgaggat cccgacgtcg
     3481 ccgcccgggc gcgcggcgcc gaggcggtgc ggcggttgtg ggcggcctgc ggactgcccg
     3541 atttccgcaa gacggggccc gagccgcacg cccggctggt gggccggctc taccttcatc
     3601 tcagcgaggg cgacgggcgc atcccgcatg gctggttcgc ggcgcagatc gcccagctcg
     3661 acaacgtcca gggcgacatc gacgcgctcg ccgaccgact cgccggagtc cgcacctggg
     3721 cctatatcgc gcaccgcgcc gactggctgg ccgacccggc ggtgatggcc gagcgcaccc
     3781 gcgaggtcga ggaacggctg tcggacgcgc tccacgagcg gctgacccag cgcttcgtcg
     3841 accggcgcac ctcggtcctg ctcaaggaca tcggccgcaa gggcgtcggc gagttcccgg
     3901 tgatcgtcga cgagcagggc gaggtcaccg tcggctcctt cccgatcggg cggatggaag
     3961 ggttcggatt cgaggtcgac ccgacggcgc gccactccga ccgcaagatg cttctggcca
     4021 ccgcggagcg gcggctcggc ggcgaatatg agaggcgcgc ggcggcgctg gcggcggaca
     4081 gcgacgatca tttcaccctg cgcacccagg ccggccaggc ggtcgcggtg ctgtggcggg
     4141 gccacgaggt ggcgcggctc gggccgggca agaacctgct cagcccgcgc gtcctggtcg
     4201 accgaaggct cgaccggctg tcggagcgcg ggcgggcgac ggtcgtcgcc cggctcgacc
     4261 tgtgggtgcg ccaccaggtc gagcgggcgc tggggtcgct tcgccaggcc ggcctggccg
     4321 cgcaggaccc gcgggcgccg gcggcggtgc gctcgatcct ggcgatgctg gtcgacgagg
     4381 gcgggatcat cgcgcgcgag gcggtcgcca agccgctcgc cgagctcgac cgggaccagc
     4441 ggcggacggt gaccaggctg aggacgcgca tcggcgccct cgatctctac atgccggacg
     4501 tgctcaagcc cgaggcgcgg cgctggcgga cggcgctccg cgccgcggcc gcgggggagc
     4561 ccatgccgga gctgccgccg gaatcggcgg tggtgctgcc cgcgcccgcc gacggcgagc
     4621 agcggcgcct cacccggctc ggcttccgcc cgctcggccc gcagatgctc cgggtcgacc
     4681 tcgtcgagcg gctggcgcgg cacgcccacg aggcccggac gggcaagccc cagccggtcg
     4741 tggacgaggc gctcgccacc tcgctcggcc tccagccgca ggcggtggcg aagctgatgc
     4801 gcgacctcgg attccgcccc gcggacggcg cccaggcgtg ggtctggcgc ggccgctcgc
     4861 cgcgccgcga cgagcctcgc cgtccggcct cgggcaacgc cttcgcggct ctggcggggc
     4921 tgaagcgtgg ctgacagcct gaggctcgac aagttcctgt ggttcgcccg gatcgtgaag
     4981 actcgggcgc tggcgcagca gctcgccgag caggggcgga tccggatc
//
LOCUS       DASVQZ010000149         3028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_898805, whole genome shotgun sequence.
ACCESSION   DASVQZ010000149 DASVQZ010000000
VERSION     DASVQZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898805"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1433)
                     /locus_tag="VF547_04120"
     CDS             complement(42..1433)
                     /locus_tag="VF547_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HEX8262042.1"
                     /translation="MVRLFKHYVPHSVLLLGVIDFLVLIGAGESAWLLRLWQIGGPAD
                     PADAQRLPHLLTFAAVLQLAMVGVGAYGVDALRSMRLAAARLVVAVSLGVLLLALVFF
                     LLPAVTFWRSNLLYAMAFALGLLFLARLAFGRAIGGEAFKRRILVLGAGPRAARLEAL
                     ARSEGAGFAIAGYVGMNDGPQAVPHSVNRDDIANLTAHVVRLRVAEVVLALEERRNAL
                     PLADLLRIKTTGVHVNEFSSFIERETGRVDLDTLNPSWLIFSDGFSSGRRLSAAGKRL
                     FDISVSAFILLVFAPLIALTALAVKLESRGPAFFRQRRVGLYGQCFELVKLRSMREDA
                     EVGGQAVWAQKDDPRVTRVGSVIRKLRIDELPQAWTVLTGKMSLVGPRPERPQFVADL
                     EARLPYYAERHVVKPGITGWAQINFPYGASVEDARQKLEYDLYYAKNYTPFLDLLILL
                     QTVRVVLWPEGAR"
     gene            complement(1548..>3028)
                     /locus_tag="VF547_04125"
     CDS             complement(1548..>3028)
                     /locus_tag="VF547_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8262043.1"
                     /translation="WAALRVARRWLPERVALPALEWRHLLWLALPLPALLYLAAGERD
                     SRYAMARFTAPGLALQGLAPATDFDRDGYSWFTPQRDAAPFDAARHPFVLDIPGNGID
                     EDGYGGDFRIQGGRAAFPAPAIPRKKHVVLVVLESTRGDAIGRKVDGRPLTPVLNALA
                     LEGSYAREAYSHVGFTTESLKSLFGGTLSPRPGAPSLFRDFKANGYRIGVFSGQPESF
                     GDISETVGMKASADVFVDAETLKAERAFGFAAKGSLLIDGKKLLRAFDRAFGQAEGWA
                     RPTFAYVNFQEAHFPYAHPGTPRILAGEPIPRGEISPANRAWVERTYWNAIAYDDRLV
                     GALIARLKRLGVWEDTLLVVTADHGESLFDDAFLGHGHVINRQQTQIPLILSERGSAI
                     DQPVGLADFRALILARLGAKVPRRTGPVFQHVSRLAAPAAIGLVAKGGVWTTFQLETE
                     EVAFGESGRRLRYRDLKPGSAEKARADSVIDEWTRQRWLARE"
BASE COUNT          508 a         1083 c         1036 g          401 t
ORIGIN      
        1 cagggcggcg agcgcgtggg tccagaagcc gagcaggacg atcatcgcgc cccctccggc
       61 caaagcacca cccgaaccgt ctggaggagg atcaggaggt ccaggaacgg cgtgtagttc
      121 ttggcatagt acagatcata ttcgagcttc tgccgcgcgt cctcgaccga ggcgccgtag
      181 gggaagttga tctgcgccca gccggtgatg cccggcttca cgacgtgccg ctcggcataa
      241 tagggaagcc gcgcctcgag gtcggcgacg aattgcgggc gttccggccg aggcccgacc
      301 aggctcatct tgccggtcag caccgtccag gcctggggca gctcgtcgat cctcaatttg
      361 cggatgaccg atccgaccct ggtcacgcgc ggatcgtcct tctgagccca caccgcctgt
      421 ccgccgacct cggcgtcctc gcgcatcgag cgcagcttca ccagctcgaa gcactggccg
      481 tagaggccga cccggcgctg gcggaagaag gcgggcccgc ggctctcgag cttcaccgcc
      541 agcgcggtca gggcgatcag cggcgcgaag acgagcagga tgaaggccga gacgctgatg
      601 tcgaacagcc tcttgcccgc cgccgacagc ctccgccccg acgagaagcc gtccgagaag
      661 atcagccagc tgggattgag ggtgtcgagg tcgacccgtc ccgtctcgcg ctcgatgaag
      721 gaggagaatt cgttgacatg gactccggtg gtcttgatcc ggagcagatc ggcgagcggg
      781 agagcgttgc gccgctcctc cagcgccagc accacttcgg cgacccgaag cctcacgaca
      841 tgggcggtga gattggcgat gtcgtcgcgg ttgaccgaat gcggcacggc ctgcgggccg
      901 tcgttcatcc cgacatagcc ggcgatggcg aagccggcgc cctcgcttcg cgccagcgcc
      961 tcgagccggg ctgcccgcgg gccggcgccg agcacgagga tccggcgctt gaacgcctcg
     1021 cccccgatcg cccggccgaa ggccagccgg gccaggaaca gcaggcccag cgcgaacgcc
     1081 atcgcgtaga gcaggttcga ccgccagaag gtgaccgccg gcagcaggaa gaagaccaga
     1141 gcgagcagca gcactccgag cgagaccgcc accaccaggc gggccgcggc gagccgcatc
     1201 gagcgcaggg cgtcgacccc gtaggcgccg accccgacca tggcgagctg gaggacggcg
     1261 gcgaaggtga gcagatgcgg caggcgctgc gcgtcggccg gatcggccgg gccgccgatc
     1321 tgccacaggc gaagcagcca cgccgactcc ccggcgccga tcagcaccag gaagtcgatc
     1381 acgcccagca ggagaacgct gtgggggacg taatgtttga acagcctgac caaggacccg
     1441 gcgcctcgcg cgatgtaaat tctgccgaca gggcgccagt gtcgctcaaa ttgacaaaca
     1501 aagcgtgaaa gggcgccgtt tccccccagg accgggcgtt cgtccgatca ctcccgcgcg
     1561 agccagcgct gcctcgtcca ttcgtcgatc accgagtccg cccgcgcctt ctcggcgctg
     1621 cccggcttca ggtcgcgata gcgcagccgc cggccgctct cgccgaaggc cacctcctcg
     1681 gtctcgagct ggaaggtcgt ccacaccccg cccttcgcga ccagcccgat cgccgccggc
     1741 gcggccagcc ggctgacatg ctggaacacc ggcccggtcc gccgcggcac cttcgctccc
     1801 agccgggcga ggatcagcgc ccggaaatcg gccagcccga ccggctgatc gatcgccgac
     1861 ccgcgctcgc tcaggatcag cgggatctgc gtctgctggc ggttgatgac gtggccgtgg
     1921 ccgaggaagg catcgtcgaa cagcgactcg ccatgatcgg cggtcaccac cagcaacgtg
     1981 tcctcccaca caccgagccg cttcaggcgg gcgatcagcg ctccaaccag ccggtcgtca
     2041 taggcgatcg cgttccagta ggtgcgctcg acccaggccc ggttcgccgg cgagatctcg
     2101 ccgcgcggga tcggctcccc ggccaggatc cgcggcgtcc ccggatgggc gtaggggaaa
     2161 tgcgcctcct ggaaattgac gtaggcgaag gtcggccgcg cccagccctc cgcttgcccg
     2221 aaggcccggt cgaacgcgcg cagcagcttc ttgccgtcga tcagcagcga tcccttggcg
     2281 gcgaagccga acgcgcgctc ggccttcagg gtctcggcgt cgacgaagac gtcggcgctc
     2341 gccttcatcc cgaccgtctc cgaaatgtcg ccgaagctct ccggctggcc cgagaacacg
     2401 ccgatccggt agccgttggc cttgaagtcg cggaacagcg acggcgcccc gggccgcggg
     2461 ctgagcgtgc cgccgaacag gctcttcagc gattcggtgg tgaagccgac atggctgtac
     2521 gcctcccgcg cgtagctccc ctcgagcgcc agcgcgttca gaaccggagt gagcgggcgc
     2581 ccgtccacct tccggccgat cgcgtcgccg cgcgtgctct cgagcacgac cagcacgaca
     2641 tgcttcttgc gggggatggc cggcgccggg aaggccgctc gaccgccttg gatgcggaag
     2701 tcgccgccat agccgtcctc gtcgatgccg ttgccgggga tgtcgagcac gaacggatgg
     2761 cgcgcggcgt cgaacggcgc cgcgtcgcgc tgcggcgtga accaggaata gccgtcgcgg
     2821 tcgaagtcgg ttgcgggcgc gagcccctgc agcgccaggc cgggcgcggt gaagcgcgcc
     2881 atcgcgtagc gcgagtcgcg ctcgccggcc gccagataga gcagcgccgg cagcggcagg
     2941 gcgagccaga gcaaatggcg ccactcgagc gccggaagcg ccacccgctc gggcagccag
     3001 cgccgcgcga cgcgcagcgc cgcccacc
//
LOCUS       DASVQZ010000150        10132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_227002, whole genome shotgun sequence.
ACCESSION   DASVQZ010000150 DASVQZ010000000
VERSION     DASVQZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227002"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF547_04130"
     CDS             complement(<1..306)
                     /locus_tag="VF547_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8262044.1"
                     /translation="MPRVLVTGAGRGIGLEFARQYAAEGWEVVATVREAQAARPLSGL
                     GVRVELLDMRDFEALAAFPDRLGGSTLDLLVANAGISRAKWIRSSDDAEAWQEVHAVN
                     "
     gene            complement(306..1241)
                     /locus_tag="VF547_04135"
     CDS             complement(306..1241)
                     /locus_tag="VF547_04135"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX8262045.1"
                     /translation="MKLMAGNSNLPLVKSIAAYLEMPVTEASVRRFADEEVFVEIHEN
                     VRGEDVFVVQSTSYPANDNLMELLICIDALRRASAKRITAVLPYFGYARQDRKPGPRT
                     PISAKLVANLITVAGANRVLSIDLHAGQIQGFFDIPTDNLYGAPVMSEDIKARFNGRA
                     ITVVSPDVGGVVRARALAKRLNNAPLAIVDKRREKPGESEVMNIIGDVGGRFCILVDD
                     IVDSAGTLCNAAAALNEAGAEDVVAYCTHGVLSGAAVQRVENSDLSELVITDSIQLSE
                     AAAASPRIRALPIAPLLAEAIRRIADESSVSSLFD"
     gene            1481..2821
                     /gene="gltX"
                     /locus_tag="VF547_04140"
     CDS             1481..2821
                     /gene="gltX"
                     /locus_tag="VF547_04140"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX8262046.1"
                     /translation="MSVVTRFAPAPTGRLHVGNIRTAVHNWLWARKHGGRFILRLDDT
                     DKERCSEAHAQGIREDLAWLGLAWDEEHRQSARFDRYEAAFAKLREVGRVYPAYETAQ
                     ELDLKRKVQLSRGKPPVYDRAALQLSDSGRARLEAEGRRPHWRFRLDHDSPIEWDDLI
                     RGRQHFDPALLSDPVVRREDGSWLYMLPSSVDDIDMGVTHVLRGEDHVANTALQIQMF
                     EALGAEPPAFAHEALLVGSEGKLSKRLGSLGADSLREQGIEPIALVAKLARLGTSLPV
                     EPVIEPGPLIETFDFATFGRAPARFDLDELAQLNARIVHQLPFEAASGRLPPGMQAQD
                     WAAIRPNLKTVDEAGDWWEILHGRVEPGDAGDDQDFLAEAARAAAEIDWLEAPWPQLV
                     HRLKERTGRSGKALFRPLRLALTGRESGPEMAALLPLIGRDETIARLGRAAATR"
     gene            complement(2944..3528)
                     /locus_tag="VF547_04145"
     CDS             complement(2944..3528)
                     /locus_tag="VF547_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262047.1"
                     /translation="MRISVWMVLLALAGCAASAGDREVAAAAAPPERSRVSAAGLGPG
                     FDSASLEREFEVARGTAALERLRSAPVSVVAAMYQGLPRPITLPDGRMGYAGPAANAV
                     IREKGRWLGWKSGELSPVPAAAAERLEAILADPRLWLEPEVFRGGGCTDAGSLQLAVR
                     QESRRKFSRQDNCDSRGLAGELGRIVLNEAVDPT"
     gene            3658..4332
                     /locus_tag="VF547_04150"
     CDS             3658..4332
                     /locus_tag="VF547_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8262048.1"
                     /translation="MMEGAFHQQKRTSPTALAVVVLMHGAALTALAMAKGEVIADAIT
                     RTKIFDVKEIQPPPEEPPKPIEKAVTPPPESVVTHVPPVVQTQPREVLVDYKPLPPVE
                     LTVLPPVREIVPPRVDPPAPPVVKTVKPARAKANLASYVSDEDYPSSAVRNEEQGTTR
                     FRLAVGPNGEVTECSIIGSSGSSALDSTTCRLMKQRARFTPARNSSGEPTSDSVTSAI
                     RWVLPE"
     gene            4377..4703
                     /locus_tag="VF547_04155"
     CDS             4377..4703
                     /locus_tag="VF547_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262049.1"
                     /translation="MPLPRPASPRALWADIRTFTSERRPHHWVALVLALLIPLAIVFV
                     FDLDGRTNIAPGPQLIYVESWPANRTDEQIKAEQKVDQAARDKAKRERQEQFKKLDEK
                     LERLGI"
     gene            4700..5671
                     /gene="ribD"
                     /locus_tag="VF547_04160"
     CDS             4700..5671
                     /gene="ribD"
                     /locus_tag="VF547_04160"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX8262050.1"
                     /translation="MTADDRWMGAAVALGERGRGNTAPNPNVGCVIVKDDVVLGRGWT
                     QAGGRPHAEAMALGEAGEAARGATVYVTLEPCCHRSERGPACADRLVEAGVARVVAAL
                     GDPDERVAGGGFERLKAAGVEVEIGPGAEQAARSMAGFLTRMRLGRPYVTLKLAMSLD
                     GKIALPSGESRWITGEDARAHVHLERARSDMILVGRGTFERDRPRLDVRLPGLEERSP
                     RRALLTRGEAVDGWTRLGSPDELFALEDVNDLLVEGGSATASAFLAADLVDRMLIYRA
                     PILIGDGKSSVGYIGLTDLADAHRRWRLADSRMLGIDRLEVYERARS"
     gene            5811..6413
                     /locus_tag="VF547_04165"
     CDS             5811..6413
                     /locus_tag="VF547_04165"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8262051.1"
                     /translation="MFTGIITDVGRVVAAEEKGDLRLRIACGYDMAGVDLGASIACSG
                     VCLTVVDKGEDWFAVDVSAETRSRSAPGLWEEGARLNLERSLRLGDELGGHIVTGHVD
                     SVGEVEAAVEVGGSVRLTVIAPAELEGYIAPKGSIALDGVSLTVNEVEPVSDGVRFAL
                     NIIPHTAAQTSFAEIAPGRKVNIEIDILARYIGRMLELRR"
     gene            6414..7526
                     /gene="ribB"
                     /locus_tag="VF547_04170"
     CDS             6414..7526
                     /gene="ribB"
                     /locus_tag="VF547_04170"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX8262052.1"
                     /translation="MERGFLSSPEELIDEARNGRMFILVDDEDRENEGDLVIPAQMAT
                     PDAINFMAKHGRGLICLALTRQRVEQLGLELMSRNNGTRHETAFTTSIEAKEGVTTGI
                     SAADRARTVSVAIDAAKGRDDICTPGHIFPLVARDGGVLIRAGHTEAAVDVSRLAGLN
                     PSGVICEIMREDGTMARMDDLVAFAQLHGLKIGTIRDLIAYRLKKDHLVEQTGEASFE
                     SRWGGTWTAKSFLNKALGTEQVALVKGKIDPARPTLVRMHQLSLFADAFGEESGRGGM
                     LRGAMKIIGEEGSGVIVVLNRAMPDALSAALNLRSGKAAAKGPDELRDYGGGAQILSA
                     LGVHDMILLTNTHHTPVALGGYGLAIVGERPIPLEE"
     gene            7528..7953
                     /gene="ribH"
                     /locus_tag="VF547_04175"
     CDS             7528..7953
                     /gene="ribH"
                     /locus_tag="VF547_04175"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX8262053.1"
                     /translation="MARLLIVEARFYDHLNDLLLAGARAAIEAAGHEHETVTVPGALE
                     VPAAIALAAESGRYEGFVALGVVIRGETYHFEIVAGESARGIMALTLDGVAIGNGILT
                     VEDEAQALARARPEEKDKGGEAAKAALAMMALRSSLRGA"
     gene            complement(8020..8976)
                     /locus_tag="VF547_04180"
     CDS             complement(8020..8976)
                     /locus_tag="VF547_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HEX8262054.1"
                     /translation="MTCTAVLAGDGPIRCLTAEEAAVYSGPGFVWIHVEGTDEEGLTL
                     LAGQAIPDVASNALVATETRPRCDVIDEGALINLRGPAAEELKDTDRLVSIRMWIRAG
                     KVNSVTRRPLLATELVRAQMEHGTISDPGDLVAAFARAISKQLDPQVAGLGDSLDDCE
                     SELDDSRALYGLRTNIARIRSEAIAFRRFVAPNRDALVTMAELGFEWLSEEDRLHIRE
                     AADRFARMTEELEAVRERAALLHEQLTDLRSEQVDSRSLVIAVFAFIFLPLTFITGLL
                     GMNVEGIPYAKEPWAFWGVVVFCLVVGAVVLAWFVRQHWLRR"
     gene            complement(8973..9833)
                     /locus_tag="VF547_04185"
     CDS             complement(8973..9833)
                     /locus_tag="VF547_04185"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX8262055.1"
                     /translation="MKKVRKAVFPVAGLGTRFLPATKAVPKEMLTVVDRPLIQYSVEE
                     ALEAGIEQMIFVTGRGKTALEDHFDIAYELEATMRARGKPLDALAGTRLPPGAVVSVR
                     QQEPLGLGHAIWCARDVVGDEPFAVFLPDEVMVGTPNCTRQLVDAYERVGGNVVAMLE
                     VPPGETHKYGVVRPGASEGGLTEILGLVEKPAPGTAPSNLMLPGRYVLQPEVMTALGE
                     IPKGAGGEIQLTDAMAALIGIQPFHGLVFDGERHDCGHMLGFVVANVALALKRSDVAP
                     ALRDYLAGLK"
     gene            complement(9864..>10132)
                     /locus_tag="VF547_04190"
     CDS             complement(9864..>10132)
                     /locus_tag="VF547_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262056.1"
                     /translation="SAMAASIVMRPLLLLPGLALLAAACGNMEPLRPKAGEAMPPRPA
                     MAARTPTTEELLTPPSNARPVRQDDGLRRSEEREDDPFDLPPTG"
BASE COUNT         1594 a         3514 c         3569 g         1455 t
ORIGIN      
        1 attgaccgcg tgcacctcct gccaggcctc ggcgtcgtcc gagcttcgga tccacttcgc
       61 tcgggagatt ccggcattgg cgacgagcag gtcgagcgtc gagccgccga ggcgatcggg
      121 gaacgccgcg agcgcctcga aatcccgcat gtcgagaagc tcgacccgca ccccgagtcc
      181 gctcagcggc cttgccgcct gcgcttcgcg gacggtggcg accacctccc agccttcggc
      241 ggcatattgg cgggcgaact cgaggccgat gccccgtccc gccccggtga cgaggactct
      301 gggcatcagt cgaacaggct ggagaccgag ctttcgtcgg cgatccgccg gatcgcctcg
      361 gcgagcagcg gggcgatcgg cagggcgcgg atccgagggc ttgcggcggc ggcctcgctg
      421 agctggatcg aatcggtgat gaccagctcc gacaggtccg aattctccac ccgctggacg
      481 gccgcgcccg acagcacccc gtgcgtgcaa taggcgacga cgtcctccgc gccggcttcg
      541 ttcagcgccg ccgcggcgtt gcacagggtg ccggcggagt cgacgatgtc gtcgacgagg
      601 atgcagaagc ggccgccgac gtcgccgatg atgttcatca cctccgattc gccgggcttc
      661 tcgcggcgct tgtcgacgat cgccaggggc gcgttgttca gccgcttggc gagcgcccgg
      721 gcgcggacga ctccgccgac gtcgggcgag acgacggtga tcgcgcggcc gttgaatcgc
      781 gccttgatgt cctccgacat gaccggggcg ccgtagagat tgtcggtcgg gatgtcgaag
      841 aagccctgga tctgcccggc atggaggtcg atcgagagga cccggttggc ccccgcgacg
      901 gtgatcagat tggcgaccag cttggccgag atgggggtgc gcgggccggg tttccggtcc
      961 tggcgggcat agccgaaata ggggaggacg gcggtgatcc gcttggccga cgcgcgtctc
     1021 agcgcgtcga tgcagatcag aagctccatc agattgtcgt tggccgggta ggaggtggac
     1081 tggaccacga acacgtcctc gccgcggacg ttctcgtgga tctcgacgaa cacctcctcg
     1141 tcggcaaagc ggcgcacgct cgcctcggtg accggcattt ccagataggc ggcgatgctc
     1201 ttcaccagcg gcaggttcga attgccggcc atcagcttca tggggagagt ctccgtcggc
     1261 gcgtggcgca actccatagg cggcccgggc gggatggcaa gccgcctcgt cctccccgtg
     1321 ggcgaagccc atggggaggg ggaccatgca aaacatggtg gaggggctgc cagcgccaat
     1381 tgcccctccg cgcttcgcgc ggtccccctc cgcatcgctg cgcgacgggg aggactgagg
     1441 cacgttgccc ctcttccttg caccccctac agcgcccccg atgagcgtcg tcacccgctt
     1501 cgccccggcg ccgaccggcc ggctgcacgt cggcaacatc cgcaccgccg tccacaactg
     1561 gctgtgggcg aggaagcatg gcggccgctt catcctccgg ctggacgaca ccgacaagga
     1621 acgctgcagc gaggcgcatg cgcagggcat ccgcgaggat ctggcctggc tcggcctcgc
     1681 ctgggacgag gagcatcggc agtcggcgcg gttcgaccgc tacgaagcgg cgttcgctaa
     1741 gctccgcgag gtcggtcggg tctatcccgc ctacgagacc gcgcaggagc tcgacctcaa
     1801 gcgcaaggtc cagctctcac gcggcaagcc gccggtctac gaccgggcgg cgctgcaatt
     1861 gagcgattcg gggcgggccc ggctcgaggc ggaggggcgc aggccccatt ggcgcttccg
     1921 cctcgaccat gactcgccga tcgaatggga cgacctcatc cgcggccgcc agcatttcga
     1981 cccggcgctg ctttccgacc ccgtggtccg gcgcgaggac ggaagctggc tctacatgct
     2041 gccgagcagc gtcgacgata tcgacatggg cgtgacccac gtcctccgcg gcgaggacca
     2101 tgtcgccaac acggcgctcc agatccagat gttcgaggcg ctcggcgccg agccgccggc
     2161 attcgcccac gaggccctgc tggtcggatc ggagggcaag ctgtcgaaac ggctgggctc
     2221 gctcggcgcc gactcgctga gggagcaggg gatcgagccg atcgcgctgg tcgccaagct
     2281 cgcccgtctc ggcaccagcc tgccggtgga gccggtgatc gagccggggc cgctgatcga
     2341 gacgttcgac ttcgcgacgt tcgggcgggc gccggcccgg ttcgacctcg acgagctcgc
     2401 ccagctcaac gcgcgcatcg tccatcaatt gccgttcgag gcggcatccg gccggctgcc
     2461 cccgggcatg caggcgcagg actgggcggc gatccggccc aatctcaaga ccgtggacga
     2521 agccggcgac tggtgggaga tcctccacgg acgagtcgag cccggagacg ccggcgacga
     2581 tcaggatttc ctcgccgaag cggcgcgggc cgccgccgag atcgactggc tcgaggcgcc
     2641 ctggccgcaa ctcgtccacc gtctcaagga acgcaccggc cggtccggca aggctctgtt
     2701 ccggccgctc cggctggcgc tgaccgggcg cgagagcggc ccggaaatgg cggccctgct
     2761 gccgctgatc gggcgcgacg agacgatcgc caggctgggg cgcgcggcgg cgacccgcta
     2821 gactttgtcc tccccagtgc caatccgttt cccggcgaag gccggggttc cagccgctca
     2881 ggcggtgcgc tgggccccgg ccttcgccgg ggaacagcgg cttcaacgtc agcaggaccg
     2941 agtctaggtc ggatcgacgg cctcgttcag cacgatccgg cccagctcgc cggcgaggcc
     3001 ccgcgaatcg caattgtcct gccggctgaa cttccgtcgg ctttcctgcc ggacggcgag
     3061 ctgaaggctt ccggcgtccg tgcagccgcc gccgcgaaag acctccggct ccagccacag
     3121 gcgcggatcc gccaggatcg cctcgagccg ctcggcggcc gccgcgggca ccggggagag
     3181 ctcgccggac ttccagccca gccatcggcc cttctcccgg atcacggcgt tcgccgcggg
     3241 tccggcatag cccatccggc cgtccgggag ggtgatcgga cggggcagtc cctggtacat
     3301 ggcggcgacg acggaaaccg gcgccgagcg cagacgctcg agcgcggcgg tcccgcgcgc
     3361 gacctcgaac tcgcgttcga ggcttgccga atcgaagccc ggtcccaagc ccgccgccga
     3421 gacccggctg cgttcggggg gagccgccgc tgcggccacc tcccggtcgc cggccgaagc
     3481 cgcgcagccg gcaagcgcga ggagaaccat ccagaccgag atcctcatgc gcgcaaaacg
     3541 cccggcaggc cgagccgctc cggcggcgga acttttgagg cttgtgtgat attgtaacat
     3601 aatgttatga gataatgtct catgagtcgt gacagcgacc gcagcaggag agaggccatg
     3661 atggaaggag ctttccacca gcagaaacgg accagcccca ccgcccttgc cgtcgtcgtg
     3721 ctgatgcacg gcgccgcgtt gaccgcgctg gcgatggcga agggcgaggt gatcgccgac
     3781 gccatcacca ggaccaagat cttcgacgtc aaggagatcc agcccccgcc cgaggagccg
     3841 cccaagccca tcgagaaggc ggtgacgccg ccgcccgagt cggtggtgac ccacgtcccg
     3901 ccggtggtcc agacgcagcc tcgggaggtc ctcgtcgact acaagccgct gcccccggtc
     3961 gagctgaccg tccttccgcc ggtgcgcgag atcgtcccgc ccagggtcga tcccccggcg
     4021 ccgccggtgg tcaagaccgt gaaacccgcg cgcgccaagg cgaacctcgc ttcctacgtg
     4081 tcggacgagg attatccgtc cagcgcggtg cgcaacgagg agcaggggac cacgcgcttc
     4141 cggctcgcgg tcggcccgaa cggggaggtc accgaatgct cgatcatcgg ctcgagcggc
     4201 tcctcggccc tcgattcgac gacctgccgg ctgatgaagc agcgcgcccg gttcacgccg
     4261 gcgcgcaaca gctcgggtga gccgacgtcg gacagcgtga ccagcgcgat ccgctgggtg
     4321 ctgccggaat agggccaggg ctggcggggg agaggcggac cgcctatctt ggcgcgatgc
     4381 cgctcccccg ccccgcttcc ccccgcgcgc tttgggccga catccggacc ttcacgtccg
     4441 agcggcggcc ccaccattgg gtcgccctcg tccttgcgct gctcattccg ctcgcgatcg
     4501 tcttcgtctt cgacctcgac ggccgtacca acatcgcgcc cgggccgcag ctcatctacg
     4561 tcgagagctg gccggccaac cgcaccgacg agcagatcaa ggccgagcag aaggtcgacc
     4621 aggccgcgcg cgacaaggcg aagcgggaac gccaggaaca gttcaagaag ctcgacgaga
     4681 agctcgagcg gctcggcatt tgacggccga cgaccggtgg atgggcgccg ccgtggcgct
     4741 cggcgagcgc ggccgcggca acaccgcccc caatcccaat gtcggctgcg tcatcgtcaa
     4801 ggacgacgtc gtcctcggcc gcggctggac ccaggccggg gggcgccctc atgccgaggc
     4861 gatggccctc ggcgaagcgg gcgaggccgc acggggcgcg acggtctacg tgaccctgga
     4921 gccctgctgc caccgcagcg agcgcggccc cgcctgcgcc gaccggctcg tcgaggccgg
     4981 cgtggcgcgc gtcgtcgcgg cgctcggcga tccggacgag cgggtcgccg gcggcggctt
     5041 cgaacggctc aaggcggccg gggtcgaggt cgagatcggc cccggcgccg agcaggcggc
     5101 gcgctcgatg gccggcttcc tgacccggat gcggctcggc cgcccctacg tcaccctcaa
     5161 gctcgccatg tcgctcgacg gcaagatcgc cctgccctcg ggcgagagcc gctggatcac
     5221 cggcgaggac gcccgcgccc acgtccatct cgagcgggcc cgaagcgaca tgatcctggt
     5281 cgggcgcggc acattcgagc gcgaccggcc caggctggac gtgcggctgc cgggactcga
     5341 ggagcgctcg ccgcgccgcg cgctgctgac ccgcggcgag gcggtcgacg gctggacccg
     5401 gctggggtct ccggacgagc tgttcgcgct ggaggacgtc aacgatctgc tggtcgaggg
     5461 cggctcggcg accgcctcgg ccttcctcgc cgcggacctg gtcgaccgga tgctgatcta
     5521 ccgggcgccg atcctgatcg gggacggcaa gtcgtccgtc ggctacatcg gcctgaccga
     5581 cctcgccgac gcccatcgcc gctggcgcct cgccgacagc cggatgcttg gcatcgaccg
     5641 gctcgaagtc tacgagcgcg cgagatcgtg atccgcctcg gtccttcgat acatccttcg
     5701 atacgccggt tcgtcaggct caccggctac tcaggatgag cggaaggact ggaattcaac
     5761 cgctcatcct gagtagggac tgagcctggc gaaggcccgt atcgaaggac atgttcacag
     5821 gaatcatcac cgatgtcggc cgcgtcgtcg cggcggagga gaaaggcgac ctgcgcctca
     5881 ggatcgcgtg cggctacgac atggccggag tcgacctcgg agcctcgatc gcctgctcgg
     5941 gcgtgtgcct gacggtcgtc gacaagggcg aggactggtt cgcggtcgat gtctcggcgg
     6001 aaacccggag ccgaagcgcg ccgggcctgt gggaggaagg cgcccgcctc aacctcgaac
     6061 gctcactccg gctcggcgac gagctcggcg gccatatcgt caccggccat gtcgactcgg
     6121 tcggcgaggt cgaggcggcg gtggaggtgg gcggctcggt gcggctgacc gtgatcgcgc
     6181 ccgccgagct ggaaggctat atcgccccga aggggtcgat cgccctcgac ggcgtgtcgc
     6241 tgacggtcaa cgaggtcgag ccggtttccg acggagtgcg cttcgccctc aacatcatcc
     6301 cccacaccgc cgcccagacc agcttcgccg agatcgcgcc ggggcggaag gtcaatatcg
     6361 agatcgacat cctcgcgcgc tacatcggcc ggatgctcga gctgaggcgc tagatggagc
     6421 ggggcttcct gtcctcgccg gaggagctga tcgacgaggc gcgcaacggc cgcatgttca
     6481 tcctcgtaga cgacgaggac cgcgagaacg agggcgacct cgtcatcccc gcccagatgg
     6541 cgactccgga cgcgatcaac ttcatggcca agcacggccg ggggctcatc tgcctggcgc
     6601 tcacccggca gcgcgtcgag cagctcggcc tcgagctgat gagccgcaac aacggcaccc
     6661 gccacgagac cgccttcacc acctcgatcg aggccaagga gggggtgacc acgggcatct
     6721 ccgccgccga tcgggcgcgc accgtctcgg tggcgatcga cgccgccaag ggccgcgacg
     6781 acatctgcac gcccggccat atcttcccgt tggtggcgcg tgacggcggg gtcctgatcc
     6841 gcgccggcca taccgaggcc gcggtcgacg tctcacggct ggcggggctc aatccgtccg
     6901 gcgtgatctg cgagatcatg cgcgaggacg gcacgatggc gcggatggac gacctcgtcg
     6961 ccttcgccca gctgcacggc ctcaagatcg gcacgatccg cgacctcatc gcctaccggc
     7021 tcaagaagga ccatctcgtc gagcagaccg gcgaggcgag cttcgagagc cgatggggcg
     7081 ggacctggac ggcgaagagc ttcctcaaca aggcgctggg caccgagcag gtggcgctgg
     7141 ttaaggggaa gatcgatcct gcccggccga ccttggtccg aatgcatcag ctcagcctct
     7201 tcgccgacgc cttcggcgag gagagcgggc gcggcggcat gctgcgcggg gcgatgaaga
     7261 tcatcggcga ggaagggagc ggagtcatcg tcgtcctcaa ccgggcgatg ccggacgcct
     7321 tgtcggcggc gctcaacctg cgctcgggca aggcggcggc gaagggcccc gacgagctga
     7381 gggactatgg cggcggcgcc cagatcctgt cggcgctcgg agtccacgac atgatcctgc
     7441 tcaccaacac ccaccatacc ccggtcgcgc tgggcggcta cggcctcgcc atcgtcggcg
     7501 agcggccgat cccgctggag gaatagaatg gccaggctgc tgatcgtcga ggcgcgattc
     7561 tacgatcatc tgaacgacct gctgctggcc ggagcccggg ccgcgatcga ggcggccggg
     7621 cacgagcacg agaccgtcac cgttcccggc gcgctcgagg tgcccgcggc gatcgccctg
     7681 gcggccgaga gcgggcggta tgaaggcttc gtcgccctcg gcgtggtgat ccgcggcgag
     7741 acctaccatt tcgagatcgt cgccggggag agcgcccgcg ggatcatggc cctgaccctg
     7801 gatggcgtcg ccatcggcaa cggcatattg accgtcgagg acgaggccca ggcgctggcg
     7861 cgcgcgcggc cggaggagaa ggacaaaggc ggcgaggccg ccaaggcggc gctggcgatg
     7921 atggcgctgc gctcgtcatt gcgaggagcg tagcgacgaa gcaatccagc ttcggccctc
     7981 ggagccgctg gattgcttcg ctacgctcgc aatgacggat cagcgcctga gccagtgctg
     8041 gcggacgaac caggcgagga cgacggcgcc cacgaccagg cagaagacca ccactcccca
     8101 gaaggcccag ggctccttcg catagggtat gccctcgacg ttcatcccga gcaggccggt
     8161 gatgaaggtc aaaggcagga agatgaaggc gaagacggcg atcaccagcg agcgtgaatc
     8221 gacctgttcc gagcgcaggt cggtgagctg ctcgtggagc agggcggcgc gctcgcgcac
     8281 cgcctccagc tcctcggtca tccgggcgaa gcggtcggcg gcctcgcgga tgtggagccg
     8341 gtcctcctcc gagagccact cgaagccgag ctcggccatg gtcaccagcg cgtccctgtt
     8401 cggcgccacg aagcggcgga aggcgatcgc ctcggagcgg atccgggcga tgttcgtgcg
     8461 aagcccgtag agcgctcgcg aatcgtccag ctcgctttcg caatcgtcca ggctgtcgcc
     8521 cagccccgcc acctgcgggt cgagctgctt cgatatcgcc cgggcgaagg cggcgacgag
     8581 atcgccgggg tcgctgatcg tgccgtgctc catctgcgcg cgcacgagct cggtggcgag
     8641 aagggggcgc cgtgtgacgg aattcacctt gccggcgcgg atccacatcc ggatcgagac
     8701 cagccggtcc gtgtccttga gctcctcggc cgccggtccc cgcagattga tcagcgcgcc
     8761 ctcgtcgatc acgtcgcagc gcggccgggt ttcggtggcg accagcgcgt tcgacgcgac
     8821 gtccggaata gcctggccgg caagcagggt caggccctcc tcgtcggttc cctcgacatg
     8881 gatccagacg aagccgggac ccgaataaac ggccgcctcc tcggcggtca ggcaccgaat
     8941 cggcccgtcg cccgcgagga cggccgtgca ggtcatttca gcccggcgag atagtcgcga
     9001 agcgccggag cgacgtcgct ccgcttcagc gccagggcga cgttggcgac gacgaagccg
     9061 agcatatggc cgcaatcgtg gcgctcgccg tcgaagacga ggccgtggaa gggttggatg
     9121 ccgatcagtg ccgccatggc gtcggtcagc tggatctcgc cgccggcgcc cttcgggatc
     9181 tcgccgaggg cggtcatcac ctccggctgg agcacgtagc ggcccggcag catcaggttc
     9241 gaaggagcgg tgccgggcgc cggcttctcg accaggccga ggatctcggt gagcccgccc
     9301 tcgctcgcgc cgggcctgac cacgccatat ttgtgggtct ccccgggcgg cacctcgagc
     9361 atcgcgacga cattgccgcc gaccctttca taggcgtcga ccagctggcg ggtgcaattg
     9421 ggcgtgccga ccatcacctc gtcgggcagg aagacggcga acggctcgtc gccgacgacg
     9481 tcgcgcgcgc accagattgc atggccgagg ccgagcggct cctgctggcg caccgacacg
     9541 accgccccgg gcggaaggcg ggtcccggcc agcgcgtcga gcggcttgcc gcgggcccgc
     9601 atcgtcgcct cgagctcgta tgcgatgtcg aaatgatcct cgagcgcggt cttgccccgg
     9661 ccggtgacga agatcatctg ctcgatcccg gcctcgagcg cctcctccac cgaatattgg
     9721 atgagcgggc ggtcgacgac ggtcagcatt tccttcggca ccgccttggt ggccggcagg
     9781 aagcgcgtgc cgaggcccgc gacggggaaa accgcctttc tgactttctt cacaggctct
     9841 ccgggaacgg ggcggccggc gggtcagccg gtcgggggaa ggtcgaaggg gtcgtcctcg
     9901 cgctcctcgg agcggcgaag accgtcgtcc tggcgcacgg ggcgggcgtt ggaaggcggc
     9961 gtcagcagct cctcggtggt cggggtccgc gccgccatcg ccggcctggg cggcatcgcc
    10021 tcgccggcct tcggccgcag cggctccata ttgccgcagg cggcggcgag aagggcgagg
    10081 ccgggaagga ggagcagggg tcgcatgacg atgctcgccg ccatagccga ag
//
LOCUS       DASVQZ010000151         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_899754, whole genome shotgun sequence.
ACCESSION   DASVQZ010000151 DASVQZ010000000
VERSION     DASVQZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899754"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="rimO"
                     /locus_tag="VF547_04195"
     CDS             complement(<1..930)
                     /gene="rimO"
                     /locus_tag="VF547_04195"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX8262057.1"
                     /translation="MATILPSPPKVGMVSLGCPKALVDSERILTKLRSDGYQLSPDYE
                     QADVVLVNTCGFLDSAKEESLAAIGEAIAENGRVIVTGCMGNEAEAIRSKFPEVLAVT
                     GPHQYEQVVGAVHVAAPIPPSAYLNLVPEGGLKLTPRHYSYLKISEGCNHRCSFCIIP
                     SIRGDLVSRRPDAVLREAEKLVAAGTKELLVISQDTSAYGVDLRHAAWPFKGGELRAH
                     MTDLARELGRLGAWVRLHYVYPYPHVDRVVPLMAEGLVLPYLDIPFQHASPPVLKAMK
                     RPANEARVLERIRGWREICPDIAIRSTFVVGFPG"
     gene            complement(968..1483)
                     /locus_tag="VF547_04200"
     CDS             complement(968..1483)
                     /locus_tag="VF547_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262058.1"
                     /translation="MDDSDLLDFTPVPVRARRDGWTPRRQYSFIQALVRGCTPGKAAA
                     LLGMTRKTAYELRTRPGAEGFAAAWTEAVARARANRIAARAAGPAGPALQGEWHPRLR
                     HGRLIGWEHRPASARSPSRLKRADRQNDRLAPGVKAGGYERRLAAEGDRSEPHARRER
                     QASRVPPRRGT"
     gene            1622..>2105
                     /locus_tag="VF547_04205"
     CDS             1622..>2105
                     /locus_tag="VF547_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8262059.1"
                     /translation="MAARPLLPRSVVALGFVSLAMDLSSEIIHALLPLFMVGTLGLSV
                     AAVGLIDGIAESSASISKVFSGWISDRLGRRKPLILLGYGLAACSKPLFALATGAVPV
                     LAARFADRIGKGIRGAPRDALIADVTEPAIRGRAFGLRQSMDTAGAFAGPLIAIGLML
                     A"
BASE COUNT          286 a          710 c          771 g          338 t
ORIGIN      
        1 gcctgggaag ccgacgacga aggtcgatcg gatcgcgatg tcggggcaga tctcgcgcca
       61 gccgcggatc cgctcgagca cccgagcctc gttggcgggg cgcttcatcg ccttcaggac
      121 cggcggcgag gcgtgctgga aggggatgtc gagatagggc aggaccagcc cctccgccat
      181 cagcgggacg acccggtcga cgtgcggata gggatagaca tagtggaggc ggacccaggc
      241 tccgagccgg ccgagctcgc gggcgaggtc ggtcatatgg gcgcggagct cgccgccctt
      301 aaagggccag gcggcgtggc gaaggtcgac cccataggcc gacgtgtcct ggctgatgac
      361 cagcaattcc ttcgtcccgg ccgcgaccag cttctccgcc tcgcgcagga ccgcgtcggg
      421 ccgccggctg accaggtcgc cgcggatcga ggggatgatg cagaagctgc agcggtggtt
      481 gcagccttcc gagatcttca gatagctgta atggcgcgga gtgagcttga ggccgccctc
      541 gggcaccaga ttgagatagg cggagggcgg gatcggcgcg gcgacgtgga cggcgccgac
      601 cacctgctca tattgatgcg gtccggtgac ggcgaggacc tcgggaaatt tcgagcggat
      661 cgcctccgcc tcgttgccca tgcagccggt gacgatcacc cggccgttct cggcgatcgc
      721 ctcgccgatc gcggcgaggc tctcctcctt ggccgaatcg aggaatccgc aggtgttgac
      781 caggaccacg tccgcctgct cgtagtcggg cgacagctgg tagccgtcgg acctaagctt
      841 ggtcaggatc cgctcggaat cgaccagcgc cttggggcag ccgagcgaaa ccatgccgac
      901 cttgggcggg gaggggagga tggtagccat gggaggcgcg ctacataggc gcccgcgccg
      961 gattttccta ggtgccgcgg cgcggcggca ctcggcttgc ctgacgctcc ctacgcgcgt
     1021 gcggctcgga cctgtcgcct tcggcggcga gccggcgctc gtagccgccc gcttttacgc
     1081 cgggcgcgag ccggtcgttc tgccggtccg cccgcttgag gcggctcggc gagcgggcgc
     1141 tcgccggacg atgctcccag ccgatcaggc gcccgtggcg caggcgcgga tgccattcgc
     1201 cctggagcgc cggcccggcg ggccccgccg ctcgagcggc gatcctgttg gcccgggcgc
     1261 gggccacggc ctcggtccag gcggcggcga agccttcggc gccgggccgg gtgcgaagtt
     1321 cgtaggcggt cttgcgagtc atgccgagca gcgccgccgc cttgccgggc gtgcagccgc
     1381 ggacgagcgc ctggataaac gaatattgcc ttcgcggcgt ccagccgtcg cggcgagcgc
     1441 ggaccggcac cggggtgaaa tcgagaagat ccgaatcgtc cactcccagc ttatggctca
     1501 gaaggggccg cgcagggaag tgttttcttt ttgttccggt caaaaaaatc ggtttttcgc
     1561 ttggcggcgc gcccggctcg cgcggctggc ggcggcgggc gccgaccatt aaggccggtc
     1621 catggccgct cgcccgcttc tcccgcgctc cgtcgtcgcg ctcggcttcg tcagcctggc
     1681 gatggacctg tcgtcggaga tcatccacgc tctgctgccc ttgttcatgg tcggaacgct
     1741 cggcctcagc gtggccgcgg tcgggctgat cgacggcatc gccgaatcct cggcctccat
     1801 ctcgaaggtc ttctccggct ggatctcgga ccggctgggc cggcgcaagc cgctgatcct
     1861 gctcggctac gggctcgcgg cctgctcgaa gccgctgttc gcgctggcca cgggggcggt
     1921 gccggtcctg gcggcgcgct tcgccgaccg gatcggcaag ggcataaggg gcgctccgcg
     1981 ggacgccctg atcgcggacg tgacggagcc ggcgatccgc ggccgcgcct tcgggctccg
     2041 gcagtcgatg gacaccgccg gcgccttcgc cgggccgctg atcgcgatcg gcctgatgct
     2101 cgccc
//
LOCUS       DASVQZ010000152         8228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_227998, whole genome shotgun sequence.
ACCESSION   DASVQZ010000152 DASVQZ010000000
VERSION     DASVQZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227998"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VF547_04210"
     CDS             <1..142
                     /locus_tag="VF547_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8262060.1"
                     /translation="LVERFFCKLKQFRRVATRFDKLARNFLAAVLLASTRLWLRTYES
                     TP"
     gene            complement(227..1222)
                     /gene="cydB"
                     /locus_tag="VF547_04215"
     CDS             complement(227..1222)
                     /gene="cydB"
                     /locus_tag="VF547_04215"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX8262061.1"
                     /translation="MDLTVIWAFLIAFAVAAYVVMDGFDLGIGILFPLFPVGRERDQA
                     MNAIAPVWDGNETWLVLGGGGLLAAFPLAYAIILPALYAPLVAMLLGLVFRGVAFEFR
                     WRDSGHRRWWDAGFFAGSLAATFAQGVTLGALVQGIAVQGRAYAGGWWDWLSPFSLLT
                     GASLVAGYALLGAAWLIWKTEGPLQDRARRQARLLLPALIAAIGSVSLYTPFVEPKYF
                     ERWFEVPGLFVAVPMPLLVAFTGFQGWRSLGRGQDWAPFLWSLALFGLTLVGLGISIW
                     PDVIPGRVTIWEAAAPPSSQLFMLVGAVVLIPLILAYTAWSYWVFRGKVGEDGYH"
     gene            complement(1367..2773)
                     /locus_tag="VF547_04220"
     CDS             complement(1367..2773)
                     /locus_tag="VF547_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX8262062.1"
                     /translation="MADGDTALLLARAQFAFTVSFHFIFPAFSIGLASYLAVLEGLWL
                     ATGRAVYLTLFRHWLKIFAIVFATGVVSGIVMSYQFGTNWSVFSDRAGPIVGPLMAYE
                     VLTAFFLEAGFLGVMLFGLERVGKRLHFLATCMVAAGTALSAFWILSVNSWMQTPAGW
                     ALNAQGQFVPAGSWLDIVFNPSFPYRLAHTVLAAYLTTALVVGGASAWHLLKGRSNPA
                     VRTSFSMAMWMAALVAPVQIVAGDLHGLNTLEHQPAKIMAMEGHFESYDKGAPLILIG
                     HPDRTAEKTHVLIEVPKLSSIILKHDPDAPLRGLKTVPKADRPPAEIVFWSFRIMVAL
                     GFLMFALGLWSLAARWRRRLHDWRWLHRFAVAMGPAGFVAVIAGWVTTEVGRQPWVIY
                     GLLRTRDAVSPIAAPGVTGSLVAFALVYFAVFGAGTTYILRLMAKAPEEGEERESSTP
                     IRTAGITPAAGVEKGRGE"
     gene            complement(2855..3175)
                     /locus_tag="VF547_04225"
     CDS             complement(2855..3175)
                     /locus_tag="VF547_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262063.1"
                     /translation="MDDNELDKLLSEALAPPERAADRGFVVRVERSMAEEDRYRKWRS
                     VFLRQLATEALALAALGGSFALIARVPAVEAMLTDAPGLIWAALLLLLLVWALMRGRG
                     NLLA"
     gene            complement(3181..3702)
                     /locus_tag="VF547_04230"
     CDS             complement(3181..3702)
                     /locus_tag="VF547_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8262064.1"
                     /translation="MTPPGDPDAVLCRRAAAGDRGAFRLLVETHERRLRAFLRQLAGP
                     DLADELAQEAFLRAWQSLHGFRDEAKFSSWLCAIGWRLFVDHKRRERSEGRKRDAAAE
                     LAEREEAPSAGARVDLGRALALLEPAEKAALVLCDGHGWSHEEAAEILRLPLGTLKGT
                     VRRAKRKCREYLR"
     gene            complement(3686..4078)
                     /locus_tag="VF547_04235"
     CDS             complement(3686..4078)
                     /locus_tag="VF547_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262065.1"
                     /translation="MPVTEMITISAIGLFFLLGFIYFLRLIQAWMLHRSLREAIKRDS
                     AHAGMLVDRIGRGDLARIEAGTDDRTGLVLVAIGIAIAGFSLIANQQDWLRLGLGAAL
                     FPTLVGAALLIRHYLLGRTAEPDVDAAG"
     gene            complement(4172..4714)
                     /locus_tag="VF547_04240"
     CDS             complement(4172..4714)
                     /locus_tag="VF547_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262066.1"
                     /translation="MLLALALALAAAVPSPPACPIERAVYRLHGAPTFSAGFARHDRR
                     NAYASDLAFWVRTPVRTYWFAFGSPNGYGGTFVSPALDPRRSAKLSDDDLYEALDRLK
                     AVEEPVSLGFHAFDSGLEAYPYPPGTDSSPPALIFVPDLGRTLWYSPVALAGGDETAR
                     DEAMPTGFFEPAGCDGPPAG"
     gene            complement(4767..5690)
                     /locus_tag="VF547_04245"
     CDS             complement(4767..5690)
                     /locus_tag="VF547_04245"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEX8262067.1"
                     /translation="MDYQGLVDGVLADVSAYKGQGTVASYIPMLAQADPNKLGLALVL
                     NDGSSYCAGDSEEPFSIQSISYVFALCLALHHVKASLWNHVGREPAGSPVNSILQLEL
                     EKGRPRNPLTSAGALVVCDQLIGADGPDVAVGELLGFLRDRSGDPKVSIDERLAMSES
                     RAGAFNRSLAHFIAAFGNLTNPVDDVLSVYYRQCSVAMSCRQLAQAALFLAFDGVDPT
                     TGDAVTIPSRSRRINALMLTSGHYDNSGDFAFRVGLPGKSAMSGGILAVIPGHGAACV
                     WSPGLNAAGTSLAGAMALERLAERSGWSVFV"
     gene            complement(5726..7399)
                     /locus_tag="VF547_04250"
     CDS             complement(5726..7399)
                     /locus_tag="VF547_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262068.1"
                     /translation="MRRALVAALLLATAACATRPAEPPSRAAAPAAEGYDLASQRAKI
                     ARIPMSPNTAFLNAEEREVVNLLIQAAELMNPIYLRQRSVENPTIRAEIARSAHPQKS
                     ALLDMFDLHFGPWDTLAENHPFYGGEAMPAGAGFYPKDLSKADFDRYLADNPGDKEAL
                     TSPYTVVRRKGGRLIAVPYSVEYRRWLEPAAKLLERAAARTSNASLRKFLSLRAKAFR
                     TNDYYESELAWMDVSGTPIEVVIGPYEVYTDTLYGTKTAFEAFVTVQDPRESAALSHY
                     KALLPDMERNLPVEDKYKNFNRPFSSPILVADQVHGGGDNVPGVQTIAFNLPNDERVR
                     EAKGAKKVILANVLGAKYDRILAPMAGLVLVPEQARLVTKKYMTQETLFHELSHSLGP
                     GTISVGGRQTTVNAELKDQYSAIEESKADVMGAYNLLYLMRRGELPIAERQQLLATYF
                     AGIFRSVRFGIDEAHGRGAAMQYSYLREKGAFDWDGGARRFRIDYDRMENGLKDLLAE
                     LVRLQGNGNYAGTKAFFDRYARLDEPARAVLATTAGIPTDIQPVYPVKV"
     gene            7463..>8228
                     /locus_tag="VF547_04255"
     CDS             7463..>8228
                     /locus_tag="VF547_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEX8262069.1"
                     /translation="MTAQTQRNRESRIFQLEPGRNCWRVSRAHRASLIVDACDYFRLA
                     RKAMLKARSQIMLMGWDIDTRVSLDGPQAKGEPPVKVGALISWIARRRPDLKIYILAW
                     DGEAYRYLGRGTTLLQLGSWARRKNIEFKLDHNHPREGSHHQKILVIDDRMAFCGGID
                     ITGSRWDTREHCDEPSERRQPTTGRAYEPWHDAIMAVDGGVAQCLGDLARLRWLAATG
                     QRLDSANPGDADHWPEELEPDFRDVDIAIARTRGEVP"
BASE COUNT         1410 a         2820 c         2812 g         1186 t
ORIGIN      
        1 tctggtcgag cggttcttct gcaagctcaa gcagttccga cgcgttgcca cccgcttcga
       61 caagctcgcc cgcaacttcc tcgctgcagt cttgctcgcc tcaacccgac tttggctcag
      121 aacttatgag tccacgccct aagccagcca ggccctcagc agccaggcca gggcggcgac
      181 gctcgtcact cccgcggccc agagcccgac gaaccaggcc agccgcctaa tgatagccgt
      241 cctcgccgac ttttccccgg aacacccagt agctccaggc ggtgtaggcg aggatcagcg
      301 ggatgaggac caccgctccg accagcatga acaactggct cgaaggcggg gcggcggcct
      361 cccagatggt gacccggccg ggaatgacgt cgggccagat cgagatgccg agcccgacga
      421 gggtgagccc gaacagagcg agcgaccaga ggaagggcgc ccagtcctgc ccgcgtccga
      481 ggcttcgcca gccttgaaag ccagtgaagg cgaccagcaa cggcatcggc acggcgacga
      541 acagtccggg aacctcgaac cagcgctcga aatatttggg ctcgacgaac ggggtgtaga
      601 ggctgacgga gccgatggcg gcgatcaggg ccggaagcag caggcgcgcc tgccgcctcg
      661 cccggtcctg aaggggtccc tccgtcttcc agatcagcca ggcggcgccg agcagggcgt
      721 agccggcgac aaggctcgcc ccggtcagca ggctgaacgg gctcagccag tcccaccagc
      781 cgccggcata ggcgcgtccc tggacggcga tgccctggac gagcgcgccc agagtcacgc
      841 cctgggcgaa ggtcgcggcg agcgagccgg cgaagaagcc ggcgtcccac cagcgcctgt
      901 ggccggaatc gcgccagcgg aactcgaagg cgacccctcg gaagacgagg ccgagcagca
      961 tcgcgacgag cggcgcgtag agcgccggga ggatgatcgc ataggccagc gggaaggcgg
     1021 cgagcagccc gcccccgccg agcaccagcc aggtctcgtt gccgtcccag accggcgcga
     1081 tcgcgttcat cgcctgatcg cgctcccggc ccaccgggaa gagcgggaag aggatgccga
     1141 tgccaaggtc gaagccgtcc atcaccacat aggccgcgac ggcgaaggcg atcaggaacg
     1201 cccagatgac ggtgaggtcc aacttaaatt cctcccccga attttcgggg gagggggacc
     1261 gcgcggagcg cggtggaggg gttctccggc gccgaagaac ccctccacca cccttcgggc
     1321 ggtccccctc cccgacaaat gtcggggagg aattcagtgc gttccctcat tcgcccctcc
     1381 ccttctccac cccggccgcc ggagtgattc cggccgtgcg gattggagtc gaggactccc
     1441 tttcctcacc ctcctccggc gccttggcca tcagccgaag gatgtacgtc gtccccgccc
     1501 cgaacaccgc gaaatagacc agcgcgaacg caaccagcga ccccgtcacg cccggcgccg
     1561 cgatcggcga caccgcgtcc cgggtccgca gcaggccgta gatcacccag ggctgccggc
     1621 cgacctcggt cgtcacccag cccgcgatga cggcgacgaa gcccgccggc cccatcgcca
     1681 ccgcgaagcg atgcagccag cgccagtcgt ggagccgtcg ccgccagcgg gcggccaggc
     1741 tccatagccc gagcgcgaac atcaggaagc cgagcgcaac catgatccgg aacgaccaga
     1801 acacgatctc cgccggcggc cggtccgcct tcggcacggt cttcagcccc cggagcgggg
     1861 cgtcgggatc gtgcttcaat atgatcgacg agagcttggg cacctcgatc agcacgtgcg
     1921 tcttctcggc ggtgcggtcg ggatggccga tcaggatcag gggcgcgcct ttgtcgtagc
     1981 tttcgaaatg gccttccatc gccatgatct tggccggctg gtgctccagc gtgttgaggc
     2041 cgtgcaggtc gccggcgacg atctgcaccg gcgcgaccag ggcggccatc cacatcgcca
     2101 tcgagaagct ggtgcgcacg gccggattgg aacggccctt cagaaggtgc caggccgagg
     2161 cgccgccgac gacgagggcc gtcgtcagat aggccgccag caccgtatgg gcgagccggt
     2221 aggggaagct cgggttgaag acgatgtcga gccagctccc cgccggcacg aactgcccct
     2281 gggcgttcag cgcccagccg gccggagtct gcatccagct gttgaccgag agtatccaga
     2341 aggccgagag cgccgtcccg gcggccacca tgcaggtggc gaggaagtgg agtcgcttgc
     2401 ccacccgctc cagcccgaac agcatcacgc cgaggaatcc ggcttcgagg aagaaggcgg
     2461 tcagcacctc ataggccatg agcggcccga cgatcggccc ggccctgtcc gagaacaccg
     2521 accagttggt gccgaactgg taggacatga cgatgcccga aacgacgccc gtcgcgaaga
     2581 cgatggcgaa gatcttcagc cagtggcgga acagcgtgag gtagaccgcc cggccggtcg
     2641 caagccagag gccctcgagg acggcgaggt aggacgcgag gccgatcgag aaggcgggaa
     2701 agatgaaatg gaacgagacg gtgaaggcga attgcgcccg ggcgaggagc agggcggtat
     2761 cgccgtcggc catgcccggg gccatagcac gctcgtcgtc gcgaacgaag gggagcagtc
     2821 tgcagagtgc atgccccagg cgtcgagggc gggttcaggc gagcaaattg ccccggccgc
     2881 gcatcagcgc ccagaccagg agcagcagca gcagcgccgc ccagatcaat cccggagcgt
     2941 cggtgagcat ggcctcgacc gccggcaccc gggcgatgag cgcgaagctc ccgccgagcg
     3001 ccgccagcgc aagtgcctcg gtcgccagct gccgcaggaa gaccgaccgc cattttcggt
     3061 agcgatcctc ctcggccatc gaccgctcga cccggaccac gaagccgcgg tcggcggcgc
     3121 gctcgggcgg cgccagcgcc tcgctgagca gcttgtcgag ctcgttgtcg tccatggcgc
     3181 tcacctcaga tactcgcggc atttccgctt ggcccgccgc accgttccct tgagggtgcc
     3241 gagcggcaat cgcaagatct ccgccgcttc ctcgtgcgac cagccatggc cgtcgcaaag
     3301 caccaaggcg gccttctcgg cgggctcgag cagggcgagg gcgcggccga gatcgacgcg
     3361 cgcgccggcg ctgggcgcct cctcgcgttc ggcgagctcg gccgcggcgt cgcgcttgcg
     3421 gccctcgctc cgctcccgcc gcttgtggtc gacgaacagc cgccagccga tggcgcacag
     3481 ccaggaggag aatttcgctt cgtcccggaa gccgtggagg gactgccagg cgcgcaggaa
     3541 ggcttcctgc gccagctcgt cggccaggtc cgggcccgcc aattgccgca ggaacgcgcg
     3601 caaccgccgc tcgtgggtct ccacgagcag gcggaaggca cccctgtctc ccgccgcggc
     3661 gcggcggcag aggacggcat cggggtcacc cggcggcgtc aacgtccggc tctgcggtcc
     3721 ggccgagcag atagtggcgg ataagcagcg ccgcgccgac cagggtcggg aagagcgccg
     3781 cgccgaggcc caggcgcagc caatcctgct ggttggcgat cagcgagaag ccggcgatcg
     3841 cgattccgat cgcgaccagc accagtccgg tgcggtcgtc cgtgcccgcc tcgatccggg
     3901 cgaggtcgcc tcgtccgatc cggtcgacca gcattccggc atgggccgag tcgcgcttga
     3961 tggcctcgcg caggctgcgg tgaagcatcc acgcctggat caggcgcagg aagtagatga
     4021 agcccaacag gaagaacagc ccgatggctg aaatggtgat catctcggtg acgggcatgg
     4081 cgaaagctcc tgatcccccc ggggcctgtc cccggtctcg aagctaggac ggacgccgcc
     4141 cccgtcctgg atcacggcgc tcgaaaaaaa atcatccggc cggaggaccg tcgcagcccg
     4201 cgggctcgaa gaatcccgtc ggcatcgctt cgtcccgcgc cgtctcgtcg cctccggcga
     4261 gagcgacggg gctgtaccac aaggtccggc cgagatccgg gacgaagatg agcgccggcg
     4321 gcgagctgtc ggtgcccggc ggatagggat aggcttcgag gccgctgtcg aaggcgtgga
     4381 agccgagcga caccggctcc tcgaccgcct tgagccggtc cagcgcttcg tagagatcgt
     4441 cgtcgctcag cttggccgac cggcgcggat cgagcgcggg cgagacgaag gtcccgccat
     4501 agccattggg cgatccgaac gcgaaccagt aggtccgaac cggcgtcctc acccagaagg
     4561 cgaggtccga cgcgtaggcg ttgcgccgat cgtgccgagc gaagccggcg ctgaaggtcg
     4621 gcgcgccgtg cagccggtag acggcgcgtt cgatcggaca ggccggcggg ctgggcacgg
     4681 cagcggcgag ggcgagggcg agggcgagga gcattgggga aggctagccg gtttgcctcg
     4741 ccgcgcgaag cccgggaagg aggcaatcac acgaacaccg accagccgct cctctccgcc
     4801 agccgctcca gcgccatcgc cccggccagc gaggtccccg ccgcgttcag ccccggggac
     4861 cagacgcagg ccgcgccgtg gccggggatg acggcgagga tgccgccgct catcgcgctc
     4921 ttgccgggca ggccgactcg gaaggcgaag tcgccgctgt tgtcgtaatg gcccgaggtc
     4981 agcatcagcg cattgatccg ccgggaccgc gacgggatgg tgacggcgtc gccggtggtc
     5041 ggatcgaccc cgtcgaacgc gaggaacagg gccgcctgcg ccagctgccg gcagctcatc
     5101 gccaccgagc attgccggta atagacggaa agcacgtcgt cgacggggtt cgtgaggttg
     5161 ccgaaggcgg cgatgaaatg ggcgaggctg cggttgaagg cgccggcgcg cgattccgac
     5221 atggcgaggc gttcgtcgat gctgaccttg gggtcgcccg accggtccct caggaagccg
     5281 agcagctcgc cgaccgcgac gtcggggccg tcggcgccga tcagctggtc gcacaccacc
     5341 agcgcgccgg cgctggtcag cggattgcgg ggccgcccct tctcgagctc gagctgcagg
     5401 atcgagttca ccggggaccc ggccggctcg cgcccgacat ggttccacag cgacgccttg
     5461 acgtgatgga gcgccaggca cagcgcgaag acgtaggaga tgctctggat cgagaagggc
     5521 tcttcggaat cgccggcgca gtagctcgag ccgtcgttca gcaccagcgc gaggccgagc
     5581 ttgttcgggt cggcctgggc gagcatcggg atgtacgacg cgaccgtgcc ctgccccttg
     5641 taggcggaga cgtccgcgag cacgccgtcg accaatcctt gatagtccat tcttttctcc
     5701 tcccctcctc tcgaaccagg aagggctaga ccttcaccgg atagaccggc tggatatcgg
     5761 tcgggatccc cgccgtcgtc gcgagcaccg cgcgcgccgg ctcgtcgagc ctggcgtaac
     5821 ggtcgaagaa ggccttggtc ccggcataat tgccgttgcc ctgaagccgg acgagctcgg
     5881 ccaagaggtc cttcaagccg ttctccatcc ggtcgtaatc gatccggaac cgccgggcgc
     5941 ccccgtccca gtcgaaggcg cccttctccc ttaggtagct gtactgcatc gccgcgcccc
     6001 gcccgtgcgc ctcgtcgatg ccgaagcgga ccgaccggaa gatgccggcg aaataagtgg
     6061 cgagcagctg ctgccgctcc gcgatcggca gctctccgcg gcgcatcagg tagagcaggt
     6121 tgtaggcgcc catcacgtcg gccttggatt cctcgatcgc gctatattga tccttgagct
     6181 cggcattgac cgtcgtctgg cggccgccga cgctgatcgt gcccgggccg aggctatgcg
     6241 acagctcgtg gaacagggtt tcctgggtca tgtacttctt cgtcaccagc cgggcctgct
     6301 cggggaccag caccagcccc gccatcggcg cgaggattcg gtcgtatttg gcgccgagca
     6361 cgttggcgag gatcaccttc ttggccccct tcgcctcgcg caccctttcg tcattgggca
     6421 ggttgaaggc gatggtctgc acccccggca cgttgtcgcc gccgccatgg acctggtcgg
     6481 cgaccaggat cggcgagctg aacggccggt tgaaattctt gtacttgtcc tcgaccggca
     6541 ggttgcgctc catgtcgggg agcagcgcct tgtagtgcga cagggccgcg ctctcgcgcg
     6601 ggtcctggac cgtgacgaag gcctcgaacg cggtcttcgt cccgtagagc gtgtctgtat
     6661 agacctcgta ggggccgatc acgacctcga tcggagtgcc gctgacgtcc atccaggcca
     6721 gctcggattc gtaatagtcg ttggtccgga atgccttggc acgcaggctc aggaacttgc
     6781 ggagcgaggc gttgctcgtc ctcgccgccg cccgctcgag cagcttggcc gccggctcca
     6841 gccaccgccg gtactcgacg ctgtagggca cggcgatcaa ccgcccgccc ttgcgccgaa
     6901 ccaccgtgta ggggctggtc agggcctcct tgtcacccgg attgtcggcc aggtagcggt
     6961 cgaaatccgc cttcgacaaa tccttagggt agaagccggc ccccgccggc atcgcttctc
     7021 ccccatagaa ggggtggttc tcggcgagcg tgtcccacgg cccgaaatgg aggtcgaaca
     7081 tgtcgagcag agcggacttc tgcggatgcg cgctgcgcgc gatctccgcg cggatcgtcg
     7141 ggttctccac cgaccgctgg cgcaggtaga tcggattcat cagctccgcc gcctggatga
     7201 gcaggttgac cacctcccgc tcctccgcgt tgaggaaggc ggtgttcggc gacatcggga
     7261 tgcgcgcgat cttggctctc tgcgacgcga ggtcatagcc ctccgcagcg ggcgcggccg
     7321 ccctcgaggg cggctcggcg ggccgagtcg cgcaggcggc ggtggcgagc agcagggcgg
     7381 cgacaagggc acgacgcatt taacttctcc tccggcgcgg atcgattgga acggctgcgg
     7441 cacgagatac gttccgaggc gcgtgaccgc tcaaacgcaa cgtaatcgcg aaagccgcat
     7501 cttccagctg gaacccggcc gaaattgctg gcgcgtctcg cgcgcccatc gcgcgtcgct
     7561 gatcgtcgac gcctgcgact atttccgtct cgcccggaag gcgatgctga aggcgcgctc
     7621 gcagatcatg ctgatgggct gggacatcga cacccgggtc tcgctggatg gtccgcaggc
     7681 caaaggcgag ccgccggtca aggtcggagc gctgatctcg tggatcgccc gcaggcggcc
     7741 ggacctgaag atctacatcc tggcctggga cggcgaagcc tatcgctacc tgggccgcgg
     7801 cacgaccctg ctgcagctcg gcagctgggc caggcgcaag aacatcgagt tcaagctcga
     7861 ccacaaccat ccgcgcgagg ggagccatca ccagaagata ttggtgatcg acgaccggat
     7921 ggccttttgc ggaggcatag acatcaccgg ctcgcgctgg gacacgcgcg agcattgcga
     7981 cgagccttcc gaacgccggc agccgacgac cggccgcgcc tacgagccgt ggcacgacgc
     8041 catcatggcg gtcgacggcg gagtcgcgca gtgtctcggc gatctcgccc ggctgcggtg
     8101 gctggcggcg acgggccagc gccttgacag cgcgaacccc ggcgacgccg accattggcc
     8161 cgaggagctg gagcccgatt tccgcgacgt cgatatcgcc atcgcccgca cccgcggcga
     8221 ggtccccg
//
LOCUS       DASVQZ010000153         7627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_132367, whole genome shotgun sequence.
ACCESSION   DASVQZ010000153 DASVQZ010000000
VERSION     DASVQZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132367"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..821
                     /locus_tag="VF547_04260"
     CDS             51..821
                     /locus_tag="VF547_04260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX8262070.1"
                     /translation="MAAESKIDPMHQFQISPLAGHLDGSPFVFTNSALWMLIVLALIW
                     IFMLGGMKRELVPGRWQAMVEGVTGFVSSMVTTNIGPEGRRYVPWVFTVFMFILFANL
                     LGMMPFALVGAQPFTVTSQFTVTGIMAIVSFSIVLVVGFWRHGFHFFSLFIPHGTPKV
                     LLPLIFLIELLSFMVRPFSLALRLFVAMIAGHILVEVFGSFVVQGINAGGVGPAISLL
                     SFLFIVGVMALEILVCAIQAYVFALLTSLYINDAVNLH"
     gene            851..1078
                     /locus_tag="VF547_04265"
     CDS             851..1078
                     /locus_tag="VF547_04265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX8262071.1"
                     /translation="MDLASAQVIGAGLAAVGVGAAAIGVGNVFGSFLESAIRNPAAAD
                     SQQGRLFIGFAAAELLGLIAFAVAMIILYAR"
     gene            1135..1629
                     /locus_tag="VF547_04270"
     CDS             1135..1629
                     /locus_tag="VF547_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX8262072.1"
                     /translation="MPQINQLADAAYSQFFWLLLVLAILYFGIGRMMLPRIQSTVEAR
                     DQRIAGDLAAAQAARSAAEETEAAYRARMDESRAEALKVAQAAKLSSARATEERVRAA
                     GEEIGARVEAAETRIRAAADAAMSEIESVAAEAAREMVERLAGVSVDGEEAARAVKSA
                     MAHG"
     gene            1622..2215
                     /locus_tag="VF547_04275"
     CDS             1622..2215
                     /locus_tag="VF547_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX8262073.1"
                     /translation="MAENSQDTHVRAAPGDGAEAAGVEHVGAPEHHPLPTALGLDAPQ
                     WIALAMVVVFAILIWKRVPALIGKALDSKIAAIRAQLDEAAQLRAEAEAIKAEYEARA
                     AQADAEAATVLERARAEAEGIVRQAESDAAALVERRSRMAEDKIAAAERAAVEEVRAK
                     AARAAAAAAESLIREKLDAGADKALVDRTIAGLGRAN"
     gene            2539..4461
                     /gene="uvrC"
                     /locus_tag="VF547_04280"
     CDS             2539..4461
                     /gene="uvrC"
                     /locus_tag="VF547_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX8262074.1"
                     /translation="MTETPPTDRFNEERSAFTVRGSDQPDLETGVAAIRKTAKSLPVR
                     PGVYRMLDAKGDVLYVGKARMLKNRVTQYTQVARLTKRLQRMVSQTRSMQIVTTNTEA
                     EALLLEAQLIKRYRPPYNVLLRDDKSFPFILLREDHPFPQIRKHRGARRHKGQYYGPF
                     ASAGSVTRTLNALQKLFLLRSCTDAFLATRKRPCLLFQIRRCSAPCVGRATPEAYAEL
                     VEDAKDFLAGKSSRVQQKLAAAMNEAAEAMDYELAAVFRDRLRSLTFIQGTQTIAAEG
                     LGDADIFALACKGGTMCVQAFFIRGGNNWGHRSFFPAHTADVPEAEVLASFLMQFYEE
                     VPPPKQVLLDREVPEVDLLCEALSERADRKVTIRVPRRGDHVRMIRQASRNAEEELDR
                     HLAETSTQAKTMRALTELFELAEPPGRIEVYDNSHVMGTNAVGAMIVAGPEGFRKNAY
                     RKFNIKREETVPGDDFAMMREVLGRRFARLEKEDPDRSRGEWPDLLLIDGGKGQLSAV
                     METLECAGIHDVPVVGVSKGPDRNAGRETLHLPNGRDFQLPPNSPVLFYIQRLRDEAH
                     RFAIGAHRQKRAKSMTASPLDEVPGIGPSRKRALLMHFGTAKAVRGAALEDLEKAPGI
                     SKAMARGIYDYFHPGG"
     gene            4646..5593
                     /locus_tag="VF547_04285"
     CDS             4646..5593
                     /locus_tag="VF547_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX8262075.1"
                     /translation="MTQVLLTVDTELNWAPHARGASWEENLALSFDPAGVGIPWQLER
                     LREHGLKACFFVDPMPAVVYGLEPVRRMVEPILAAGQEVQLHLHPVWQSVAEGVAEGA
                     RFELTCFDAEDQLDLIETARDLLIEAGAPPPIAFRSGGYAADVRTLAALTQAGIRFDS
                     SHNGSHHPDPSALPLEPDLIGPADLGGLIEVPVTQIGDVGERLRHLQLCAVSGEEIEA
                     ALRHALRSGHPLVTLVSHSFELASGDRRRPNRTLCRRFERLCAFLAEHCDSLPTAHFG
                     DLGALPLRAEGEPLPPQPWRRARRMAQQAWSNALYERSL"
     gene            5765..6496
                     /gene="recO"
                     /locus_tag="VF547_04290"
     CDS             5765..6496
                     /gene="recO"
                     /locus_tag="VF547_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX8262076.1"
                     /translation="MHLHTPAIICAVRPHGEHGAIVRALTPADGLQAGYVRGGRSRRL
                     RPILLAGNLVEAEYRARTDDQLAQLSVELAVSRAPLLGEALPAAAIEWTTALAATALP
                     EGQPYPRLYQALDGLLGAIAASPSARAWAPSLVRYELFVLAELGFGLDLSECAATGAT
                     DGLAFVSPKSGRAVSAVAAGAWSDRLLPLPPLLLGESGGDWPDILAGLRITGHFLARD
                     VLIERQAEVLAARERLLDRLKRAGG"
     gene            complement(6635..>7627)
                     /gene="wecB"
                     /locus_tag="VF547_04295"
     CDS             complement(6635..>7627)
                     /gene="wecB"
                     /locus_tag="VF547_04295"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX8262077.1"
                     /translation="LTGQHRGLRGFFDLPEGSVRELGFDPSGRTQRDLRTALRRRLCG
                     DLRSERADLVLVQGDTTSAVAGAYAALDCGLPVGHVEAGLRSFDLRQPHPEEGHRIVI
                     DSLSDLLFAPTQAAAANLRREGRARGRIWVTGNSGIDSLLRARARLEPLRPEPQARRR
                     RILVTCHRRENQGRPVLRVCAALRRMVSEMPVRIVAALHPNRHVRRAVETALAGAEHI
                     ELREPLGYEEMVRLMARSWLILSDSGGLQEEAPALGTPLLVLRDVTERPEALATGNVE
                     LVGTDAGRILAAVAALLDCPERHARMSRPAFPFGDGRASERIIAAIEEWLAERR"
BASE COUNT         1144 a         2759 c         2594 g         1130 t
ORIGIN      
        1 tggaagattt cgaagcaaaa gcccgaatga cgggccgagg gacgtagaga gtggcggccg
       61 aaagcaagat cgacccgatg caccagttcc agatcagccc gctcgcgggc catctggacg
      121 gaagcccgtt cgtcttcacc aacagcgccc tgtggatgct gatcgtcctc gcgctgatct
      181 ggatcttcat gctgggcggg atgaagcgcg agctcgtccc cggccgctgg caggcgatgg
      241 tcgagggcgt gaccggcttc gtcagctcga tggtgacgac caacatcggc cccgagggac
      301 gccggtacgt cccctgggtg ttcaccgtct tcatgttcat cctgttcgcc aacctgctgg
      361 ggatgatgcc cttcgccctg gtcggagcgc agcccttcac cgtcaccagc cagttcaccg
      421 tcaccgggat catggcgatc gtcagcttct cgatcgtgct ggtcgtcggc ttctggaggc
      481 acggcttcca cttcttctcg ctcttcatcc cgcacggcac gcccaaggtg ctgctgccgc
      541 tcatcttcct gatcgagctg ctctcgttca tggtccggcc gttcagcctc gcgctccggc
      601 tcttcgtggc gatgatcgcc ggccacatcc tcgtcgaggt gttcggaagc ttcgtcgtcc
      661 aggggatcaa cgccggcggg gtcgggccgg cgatcagcct gctgtcgttc ctgttcatcg
      721 tcggcgtgat ggcgctcgag atcctggtct gcgcgatcca ggcctatgtc ttcgcccttc
      781 tgacgtcgct ctacatcaac gacgccgtca accttcacta gcttcaaacg acttacccaa
      841 ggagaatatc atggacctcg catctgccca ggttatcggc gccggcctcg ccgcggtcgg
      901 agtcggcgcc gccgcgatcg gcgtcggcaa cgtcttcggc tcgttcctcg agagcgcgat
      961 ccgcaacccg gccgcggccg acagccagca gggccgcctg ttcatcggct tcgccgccgc
     1021 cgagctgctc ggcctcattg ccttcgccgt ggcgatgatc atcctctacg cccgctagga
     1081 taaggagggg acacgtacct tttccgcgaa aaggtacgtg tccccgttta cgccatgcct
     1141 cagatcaatc agctggccga cgccgcctat tcgcagttct tctggctgct gctggtgctc
     1201 gccatcctct atttcggcat cggccggatg atgctgccca ggatccagtc gaccgtcgag
     1261 gcgcgcgacc agcgcatcgc cggggatctc gccgcggccc aggccgcgcg ctcggccgcc
     1321 gaggagacgg aggcggccta tcgcgcccgc atggacgaga gccgcgccga ggcgctgaag
     1381 gtcgcgcagg ccgccaagct gtcgagcgcc agggcgaccg aggagcgggt gcgggcggcc
     1441 ggggaggaga tcggcgcccg ggtcgaggcg gccgagaccc ggatccgcgc cgccgcggac
     1501 gccgcgatga gcgagatcga gagcgtcgcg gccgaggccg cgcgcgagat ggtcgagcgc
     1561 ctggccgggg tctcggtcga cggcgaggaa gccgcccggg cggtcaagtc ggcgatggcc
     1621 catggctgag aacagccagg acacccatgt gagggcggcg cccggcgacg gcgccgaggc
     1681 cgccggagtc gagcatgtcg gagcgcccga gcatcacccg ctgcccaccg ccctgggcct
     1741 cgacgcgccg cagtggatcg cactggcgat ggtggtggtc ttcgccatcc tcatctggaa
     1801 gcgggttccg gccctgatcg gcaaggcgct cgattccaag atcgccgcga tccgggccca
     1861 gctcgacgag gccgcgcagc tgcgcgccga ggcggaggcg atcaaggccg aatatgaggc
     1921 ccgcgccgcc caggcggacg ccgaggccgc gaccgtgctc gagcgcgccc gagccgaggc
     1981 cgaggggatc gtccgccagg cggagagcga cgcagcggcg ctggtcgagc gtcgaagccg
     2041 gatggcggag gacaagatcg ccgccgccga gcgcgccgcc gtcgaggagg tccgcgccaa
     2101 ggccgcgagg gccgctgcgg cggcggccga aagcctgatc cgcgagaagc tcgacgccgg
     2161 ggcggacaag gccctggtcg accggacgat cgcggggctg ggacgggcga actgaaattc
     2221 ctccccgcga tttcgcgggg agggggaccg cacgaagtgc ggcggagggg ttcttcggag
     2281 tccgaaagga cccctccacc atgcttcgca tggtccccct ccccggcaaa ggccggggag
     2341 gattatcttt tcccagccct tctccgccac gccgccgcca gggccgtcgc gaagaccttc
     2401 ccgccgacgc ggaagtccgc atggcccatg tgggcgccct cctgcgcgcc cggaaaggac
     2461 agggcgattc ggcggaaatc gtcggttgtc ggcatggctc accttaacat ggcccgcgcc
     2521 ggcatctaga tcggccctgt gaccgagacc ccgccgaccg accgcttcaa cgaggagaga
     2581 tccgccttca ccgtgcgcgg gtcggaccag ccggacctcg agacgggcgt cgccgcgatc
     2641 cgcaagaccg cgaagtccct gccggtccgc cccggggtct atcggatgct cgatgccaag
     2701 ggcgacgtcc tctacgtcgg caaggctcgg atgctgaaga accgggtcac ccaatatacc
     2761 caggtggcgc ggctgacgaa gcggctgcag cggatggtct cgcagacccg atcgatgcag
     2821 atcgtcacca ccaacaccga ggcggaggcg cttctgctcg aggcgcagct gatcaagcgc
     2881 taccggccgc cctacaacgt cctgctgagg gacgacaaaa gcttcccctt catcctcctt
     2941 cgcgaggacc atcccttccc gcagatccgc aagcatcgcg gcgcccgccg ccacaagggt
     3001 cagtattacg gcccgttcgc cagcgcgggg tcggtcaccc ggaccttgaa cgcgctccag
     3061 aagctgttcc tgctgaggag ctgcaccgac gccttcctcg ccacccgcaa gcggccctgc
     3121 ctcctcttcc agatccgccg ctgctcggcc ccctgcgtcg gccgggccac gcccgaggcc
     3181 tatgccgagc tggtcgagga cgcgaaggac ttcctcgccg gcaagtcgag ccgggttcag
     3241 cagaagctcg cggcggcgat gaacgaggcg gcggaggcga tggactacga gctcgccgcc
     3301 gtgttccgcg accggctgcg ctcgctgacc ttcatccagg gcacccagac catcgccgcc
     3361 gagggcctcg gcgacgccga catcttcgcg ctcgcctgca agggcgggac gatgtgcgtc
     3421 caggccttct tcatccgcgg cggcaacaat tgggggcacc ggagcttctt cccggcccac
     3481 accgccgacg tgcccgaggc ggaggtgctc gccagcttcc tgatgcagtt ctacgaggag
     3541 gtgccgccgc ccaagcaggt gcttctcgac cgcgaggtgc cggaggtcga cctgctgtgc
     3601 gaggcgctgt ccgagcgggc cgaccgcaag gtgacgatcc gggtgccccg ccgaggcgac
     3661 catgtccgga tgatccgcca ggcgtcgcgc aacgccgagg aggagctgga ccggcacctc
     3721 gccgagacca gcacccaggc gaagacgatg cgggcgctga ccgaattgtt cgagctggcc
     3781 gagccccccg gccggatcga ggtctacgac aacagccacg tcatgggcac caacgcggtc
     3841 ggggcgatga tcgtcgccgg gccggagggc ttccgcaaga acgcctacag gaagttcaac
     3901 atcaagcgcg aggagacggt gcctggcgac gatttcgcga tgatgcgcga ggtgctcggc
     3961 cggcgcttcg cccggctcga gaaggaggat ccggaccgga gccgcggcga atggccggac
     4021 ctgctgctga tcgacggcgg caagggccag ctttccgccg tcatggagac cctggaatgt
     4081 gcaggcatcc acgacgttcc ggtcgtcgga gtgtcgaagg ggcccgaccg caatgccggg
     4141 cgcgagacgc tgcacctgcc caatggccgc gacttccagc tgccgccgaa ctcgccggtc
     4201 ctcttctaca tccagcgcct tcgcgacgag gcccaccgct tcgccatcgg cgcccaccgc
     4261 cagaagcgcg cgaagagcat gaccgcgagc ccgctcgacg aagtccccgg catcggcccg
     4321 agccgcaagc gcgccctgct catgcatttc ggcaccgcca aggcagtgcg cggcgcggcg
     4381 ctcgaggatc tggaaaaggc cccgggcatc tcgaaggcga tggcgcgcgg catctacgac
     4441 tatttccacc cgggcggcta atgccaattc ctcccccgca tttgcggggg agggggacca
     4501 ggcgaagccg gggggagggg ttcttcgggc gttggaagga acccctccac cgcgctccgc
     4561 gcggtccccc ttcccgagaa atctcgggga ggaattttgc agcgcgctca ccatatgttc
     4621 acccgcgccg ggtagaccgc tcccgatgac ccaggtcctg ctcaccgtcg acaccgagct
     4681 caattgggcg cctcatgccc gcggcgcctc gtgggaggag aatctggcgc tctccttcga
     4741 tcccgccggg gtcggcatcc cgtggcagct ggaaaggctt cgcgagcacg gcctcaaggc
     4801 ctgcttcttc gtcgatccga tgccggcggt ggtctacggg ctcgagccgg tgcggcgaat
     4861 ggtcgagccg atcctcgccg ccggccagga ggtccagctc cacctccacc cggtctggca
     4921 gagcgtcgcc gagggcgtcg ccgagggcgc ccgcttcgag ctgacctgct tcgacgccga
     4981 ggaccagctc gacctgatcg agaccgcccg cgaccttctg atcgaggccg gcgcgccgcc
     5041 gcccatcgcc ttccgctccg gcggctatgc cgccgacgtg cgcacgctgg ccgccctcac
     5101 ccaggcgggc atccggttcg acagcagcca caacggcagc caccatcccg atcccagcgc
     5161 gcttccgctc gagccggacc tgatcggccc cgccgacctg ggcggcctga tcgaggttcc
     5221 ggtgacccag atcggcgacg tcggcgagcg gctgcgccac ctccagctct gcgccgtctc
     5281 aggcgaggag atcgaggccg cgctccgcca cgcgctccgt tccggccacc cgctcgtcac
     5341 cctggtcagc cacagcttcg agctggcgag cggcgaccgg cgccgcccca accgcaccct
     5401 gtgccgccgc ttcgaacggc tctgcgcctt cctcgccgag cattgcgaca gtctgccgac
     5461 cgcccatttc ggcgacctcg gggcgctgcc gctgcgcgcc gaaggcgagc cgctgccgcc
     5521 ccagccctgg cgccgcgccc gccgcatggc gcagcaggcc tggtcgaacg cgctctacga
     5581 acggtcgctc tgacctgtcc ttcgatacgc cgcttcggct tcgctcagcg gctactcggg
     5641 atgagcggtt gtgctttttc cgctcatcct gagcagggac ctggcgaagg cccgtatcga
     5701 aggaaggacc gggctgagag aagcggttta gctcggccga acatcgcgct agtctgcgcg
     5761 gcccatgcac ctccacaccc cggcgatcat ctgcgcggtc cggccgcatg gcgagcatgg
     5821 agccatcgtc cgcgccctca cgccggccga cggcctgcag gcgggctacg tccgcggcgg
     5881 ccgctcgcgc cggcttcggc cgatccttct cgccggcaac ctcgtcgagg ccgaatatcg
     5941 cgcccgcacc gacgaccagc tcgcccagct cagcgtcgag ctggcggtca gccgggcccc
     6001 gctcctcggc gaggcgctgc cggcggccgc gatcgaatgg acgacggccc tggccgcaac
     6061 cgctctgccg gaggggcagc cctatccgcg cctctaccag gcgctcgacg gcctgctcgg
     6121 cgccatcgca gcctcgccga gcgcccgcgc ctgggcgccg tcgctggtcc gctacgagct
     6181 gttcgtcctc gccgagctcg gcttcggcct cgacctttcg gaatgcgccg cgaccggcgc
     6241 gaccgacggc ctcgccttcg tcagcccgaa aagcggccgg gcggtgagcg cggtggcggc
     6301 gggggcctgg agcgaccggc tgctgccgct gccgccgctg cttctcggcg agtccggagg
     6361 cgactggccg gacatcctcg ccggccttcg gatcaccggc catttcctcg cccgggacgt
     6421 cctcatcgag cgccaggccg aggtgctggc ggcgcgggag cggctgctcg accggctgaa
     6481 gagggccggg ggttaaattc ctcccctgca ttttgcaggg aagggggacc aggcgaagcc
     6541 tggtggaggg gttcttcgga cgtcggaaaa aaccctccac cgcgcttcgc gcggtccccc
     6601 tccgccggaa agccggggga ggaatcaggg ccgcctatcg ccgctccgca agccattcct
     6661 cgatggcggc aatgatccgc tccgaggccc ggccgtcgcc gaacggaaag gcggggcgcg
     6721 acatccgggc atgcctttcg gggcagtcga gaagcgcggc gacggcggcg aggatgcgcc
     6781 ccgcgtcggt gccgaccagc tcgacattgc cggtggccag cgcctccggc cgctcggtga
     6841 cgtcgcggag caccagcagc ggcgtgccga gcgccggcgc ctcctcctgc agcccgccgc
     6901 tgtcgctgag gatcagccag ctccgggcca tcagccgcac catctcctca tagccgagcg
     6961 gttcccggag ctcgatatgc tccgcgccgg cgagcgccgt ctcgaccgcg cggcgcacgt
     7021 ggcggttggg gtggagggcc gccacgatcc gcaccggcat ctcgctcacc atccgcctca
     7081 gcgccgcgca gactcgaagg accggccggc cctggttttc gcgccggtgg caggtgacga
     7141 ggatccgccg gcggcgggct tgcggttccg gccggagcgg ctccagccgc gcccgggccc
     7201 tcagcaggga gtcgatgccg ctgttgcccg tcacccagat cctgcctctg gcccgccctt
     7261 cccggcgcag gttggcggcg gcggcctggg tcggcgcgaa cagcaggtcg gaaagcgagt
     7321 cgatgacgat ccggtggcct tcctcgggat ggggctggcg gaggtcgaac gagcgcaggc
     7381 cggcctcgac atggccgacc ggcagcccgc agtcgagcgc cgcataggcg ccggcgacgg
     7441 cgctggtcgt gtcgccctgg acgagcacca gatcggcccg ctcgcttcga agatccccgc
     7501 acagccgccg cctgagcgcg gtccgcaagt cccgctgggt gcggccgctg ggatcgaagc
     7561 cgagctcgcg gacgctgccc tcgggcaggt cgaaaaagcc gcgcaggccg cgatgctggc
     7621 cggtcag
//
LOCUS       DASVQZ010000154         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_32399, whole genome shotgun sequence.
ACCESSION   DASVQZ010000154 DASVQZ010000000
VERSION     DASVQZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32399"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1460)
                     /locus_tag="VF547_04300"
     CDS             complement(132..1460)
                     /locus_tag="VF547_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8262078.1"
                     /translation="MATGDERSGLNRAIWAIALPSMLTNVATALFGLADLWVIGRLGE
                     AEPQAGVELGAKFMMGLLIVFNFLRTGTIALTAQAAGRRDEEAQAATLARALGVSLAI
                     GLALLALKPVVIPWGMELLHARGAVAREAGLYVGIRYWGGLLWLANVALMGWLIGRRR
                     VRAVLWTEIGSNLVHIALDIAFVLGFGWGVAGVAAATLVSEGGKTLVLAAVTARQQPA
                     RRALAAAARRETWSAAAMGALFRLNRDLFLRTLLLTAAILLLTRQGAQQGPMVLAANG
                     ILYQLFILSALILDGFESAAQVLCGEAVGARDRARFDALVRRLVLWGLGGGAVFSLVY
                     ALLGARFAASFSTDPAVVATTLEYVPWAVLLPLLGVTSYVFDGVYIGATWTRAMLLTM
                     IAAIAVYSALLIVAAPLGNHGLWLAFSLFLIARAAAQAALLPRLRRGTFG"
     gene            1629..2282
                     /gene="fsa"
                     /locus_tag="VF547_04305"
     CDS             1629..2282
                     /gene="fsa"
                     /locus_tag="VF547_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX8262079.1"
                     /translation="MKFFVDTADTAEIRDLAETGLLDGVTTNPSLIHKAGRDFLEVVR
                     EICSIVEGPVSAEVVALDHKEMMREADVLRKIAGNIAVKVPLTVDGLKTCKALTGEGT
                     MVNVTLCFTANQALLAAKAGATFISPFVGRHDDTGFDGMQIISEIRTIYDNYDFGTQI
                     LVASIRHPIHVLQSAMIGADVMTAPPAVIRQLFKHPLTDKGLEGFAADWAKTGQKIG"
     gene            2444..3004
                     /locus_tag="VF547_04310"
     CDS             2444..3004
                     /locus_tag="VF547_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX8262080.1"
                     /translation="MGKVINFEDRAVAKLRAQVAAAEEANQDLIAFARGHSGAVASIH
                     EAVLAAIEAEGFDHLIHIVTQEWPQILGLDAVAVALFAGDKGVRADASGLQFVDARVV
                     RRSLEGLDGVVLRGVGRGHPLFGPACELIRAEALVRLDSEPPLPSGLLALGQREAQGF
                     ETRHGSELLMFLGRVLTRSMGRWLIP"
     gene            2989..3126
                     /locus_tag="VF547_04315"
                     /pseudo
     CDS             2989..3126
                     /locus_tag="VF547_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase XerC"
     gene            complement(3121..3390)
                     /locus_tag="VF547_04320"
                     /pseudo
     CDS             complement(3121..3390)
                     /locus_tag="VF547_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          551 a         1230 c         1146 g          463 t
ORIGIN      
        1 gcttgtccgg aagaagcggc tcaatcagcc gccattccgc ttcactaaga tcgtaccgtc
       61 ccatccaagg ctcctttcat gagccttgaa tcaaacaccg acccgaccgg caagctttat
      121 gagtacaagc cctagccgaa ggtcccgcgc ctcagccgcg gcagaagcgc cgcctgggcg
      181 gcggcccggg cgatcaggaa gaggctgaag gcgagccaca gcccgtgatt gccgagcggt
      241 gccgcgacga tgagaagcgc cgaatagacg gcgatcgccg cgatcatggt gagcagcatc
      301 gcccgcgtcc aggtcgcgcc gatatagact ccgtcgaaga cgtaggaggt gaccccgagc
      361 agcggcagaa gcaccgccca ggggacatat tccagggtcg tcgcgaccac cgccggatcg
      421 gtgctgaagc tggcggcgaa gcgcgcgccg agaagggcgt agaccaggct gaacaccgcg
      481 cctccgccga ggccccaaag caccagccgc ctgaccaggg cgtcgaagcg ggcgcggtcc
      541 cttgccccga ccgcctctcc gcacagcacc tgggctgcgc tctcgaagcc gtcgaggatc
      601 agggccgaga ggatgaacag ctggtagagg atgccgttgg cggccagcac catgggcccc
      661 tgctgggcgc cttgccgggt gagcaggagg atggcggcgg tgagcagcag cgtgcgaagg
      721 aagaggtcgc ggttgagccg gaacagagcg cccatcgccg cggcgctcca ggtctcgcgc
      781 cgcgccgccg ccgccagcgc ccggcgcgcg ggttgctggc gagcggtcac ggccgccagg
      841 acgagcgtct ttccgccctc cgagacgagg gtggcggcgg cgaccccggc cacgccccag
      901 ccgaagccga ggacgaaggc gatgtcgagc gcgatatgga cgaggttcga gccgatctcg
      961 gtccaaagca ccgcccgcac ccgccggcgg ccgatcagcc agcccatcag ggcgacgttg
     1021 gcgagccaga gcaggccgcc ccaatagcgt atcccgacat agaggccggc ctcccgcgcc
     1081 actgcgccgc gggcgtggag cagctccatg ccccacggaa tcacgaccgg cttcagcgcc
     1141 agcagggcca gcccgatcgc cagcgagact ccgagcgccc gcgccagcgt cgccgcctgc
     1201 gcctcctcgt ctcgccggcc ggcggcctgg gcggtgagcg cgatggtgcc ggtcctcagg
     1261 aagttgaaga cgatgagcag gcccatcatg aacttggcgc ccagctccac ccccgcttgc
     1321 ggctccgcct cgccgagccg gccgatcacc cacagatcgg cgaggccgaa cagggccgtg
     1381 gcgacgttgg tcagcatcga gggcagggcg atcgcccaga tcgcccggtt cagtccgctt
     1441 cgctcatccc cggtcgccaa gtccgccccc ttccgtcatt gcgaggagcg aagcgacgcg
     1501 gcaatccagc tgcggcgctt gcggagattc cgcactggat tgcttcgcct cgctcgcaat
     1561 gacggtgttt cactatcggt cgaacctgcc ctagaaggcc gctgaaaccc agtcccaagg
     1621 accgtcccat gaaattcttc gtcgacaccg ccgacaccgc ggaaatccgc gacctcgccg
     1681 agaccggcct gctcgacggg gtcaccacca acccgtcgct gatccacaag gccggccgcg
     1741 acttcctcga ggtggtccgc gagatctgct cgatcgtgga gggcccggtc tcggccgagg
     1801 tggtcgcgct cgaccacaag gagatgatgc gcgaggccga cgtgcttcgc aagatcgccg
     1861 gcaacatcgc ggtcaaggtg ccgctcaccg tcgacgggct caagacctgc aaggcgctca
     1921 ccggcgaggg gacgatggtc aacgtcacct tgtgcttcac ggccaaccag gcgctgctcg
     1981 ccgccaaggc cggcgcgacc ttcatctcgc ccttcgtcgg ccgccacgac gacaccggct
     2041 tcgacggcat gcagatcatc tccgagatcc gcaccatcta cgacaattac gatttcggga
     2101 cgcagatcct ggtcgccagc atccgccacc cgatccacgt cctccagtcg gcgatgatcg
     2161 gcgccgacgt catgacggcg ccccccgcgg tcatccgcca gctcttcaag cacccgctca
     2221 ccgacaaggg cctggaaggc ttcgccgccg actgggcgaa gaccggccag aagatcggat
     2281 gatcctcccg ggacgaggag ggggaccgcc gccgaaggcg gtgacggaag gggctcggcg
     2341 cgtgtcgcgg gcgcccctcc accagcctgc cgccggtccc cctccccgtc ccggggagga
     2401 attgacgtag cttcttaacc ttgttcgtat tagcttcggc gcgatgggca aggtgatcaa
     2461 cttcgaagac cgggcggtgg ccaagctgcg tgcccaggtg gcggcggcgg aggaggccaa
     2521 ccaggacctg atcgccttcg cccgcggcca ttcgggcgcg gtcgcctcga tccacgaggc
     2581 ggtgctggcg gcgatcgagg cggaaggctt cgaccacctc atccacatcg tcacccagga
     2641 atggccgcag atcctcggcc tggatgcggt ggcggtcgcg ctcttcgccg gcgacaaggg
     2701 cgtccgcgcc gacgcgtcgg gactccagtt cgtcgacgcc cgcgtcgtcc gccgctcgct
     2761 cgaggggctc gacggcgtcg tgcttcgagg cgtgggccgg ggccatccgc tgttcgggcc
     2821 ggcctgcgag ctgatccggg cggaggccct ggtcaggctc gacagcgagc cgccgctgcc
     2881 cagcgggctg ctcgcgctcg gccagcgcga ggcgcagggc ttcgagaccc gacacggctc
     2941 ggagctgctg atgttcctgg gccgggtcct cacccgctcg atgggccggt ggctgatccc
     3001 ctaggcggcc acccggccgc cgccgccgct tttcgctggc agcagcatct cgccaacgat
     3061 cgccgccgct ccgcccatac ggtgcgcgcc tattgcgcga ccgcgcatcg cctgatcggc
     3121 ttcctcggcc agggcgaccg cgtcgtccgg cgcgaccgga cgcggcacgc tgcgcggccg
     3181 tttcggcccc ttgagccggg gcaaggcggg ctcccctccc cgctcggccg cggcgaaggc
     3241 gaggaagccc cgaacggccg agagctcgcg cgccgccgag ctgttgccca gtccttcgcc
     3301 gcgccgatgg gcgagaaagg cgcgcaggtc cttcgcctcg aggccggcga gcgccggccc
     3361 gtcgaccggg ccgccgagat gaccggcgag
//
LOCUS       DASVQZ010000155        21655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_228268, whole genome shotgun sequence.
ACCESSION   DASVQZ010000155 DASVQZ010000000
VERSION     DASVQZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 21655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21655
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228268"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1537
                     /gene="dacB"
                     /locus_tag="VF547_04325"
     CDS             62..1537
                     /gene="dacB"
                     /locus_tag="VF547_04325"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX8262081.1"
                     /translation="MTRPSTALRAAALLLVLAGPSAAAAAPSEPLQQRVEAKLGEAGP
                     GVRFGLVVADAAGRELVAVAPEGRFIPGSNIKMFTTAAAFATLPSLDAPDSAGGAAVR
                     LEPAGRGLADVVLEGRGDARLSSAAGCVQDCLSALADAVAARTRRVRDVIGDDSLFPD
                     QRWSPGMSWNNIPTRSGTAASALSLDDNELPLRVFATTPGAPPRLELLDYYEVHNRAV
                     TVAAGPTELSFDRFPGSRLVRLEGRIAAGAEPEWLRLGIDDPAHYAAWRLKALLEERG
                     VRVSGTVAVRHRPLRPADDPAVRNGAPAARPPEAATVLARLVPPPLAEDLAAISKASQ
                     NLHSELLLRRIGRQQGSGSIADGLAAVHAMLAAAGVPRTGYDVSDGSGMSSYNRIAPR
                     AMVAFLRWAAAQPWGAAWRATLPIGGVDGTLDGRFKGSPLQGRIFAKTGTLNASNALS
                     GFMIARSGRTLLFSAFANDVPGGVAATAAMDSALVLIAEEN"
     gene            complement(1672..2673)
                     /locus_tag="VF547_04330"
     CDS             complement(1672..2673)
                     /locus_tag="VF547_04330"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8262082.1"
                     /translation="MIEAAFAPAAAAVAEGRIPGAALGIVRSDGRRSVKVSGMAALEP
                     APEALSEDHWFDLASLTKVIATTTMILRLADEGRLGLDRPLTDAIPDLRQYDPDGAAE
                     RRLTFRDCLAHRTFLPATEPIYTYGDDPARLRAFVLQREWRRGPPVYSDINFILLGIA
                     VERITGAPLSAWPLGAGLGYGPPPGPAVATERCAWRGRVLAGEVHDENAAALGGEAGH
                     AGLFGTVAGVLDFALGLLDGSGASPAMLEAIRTRCRDDRTCGWERRFDGWHGGDSCSP
                     ATIGHTGFTGTGLWVDFERGLAWTLLTNRVHPTRHRETGIVALRRATGNAVIGAWDG"
     gene            complement(2670..4217)
                     /locus_tag="VF547_04335"
     CDS             complement(2670..4217)
                     /locus_tag="VF547_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX8262083.1"
                     /translation="MAEAAAIPPPVVRSRLGRMALYVLLGFSAGLPFYMFSTVLALRL
                     QSHGVVLVVIGFFTWVQLLPTFKFLWAPALDRFDVPGFARFWGKRRGWIMLSQLGIFT
                     SMVAMALTSSDSNLAVTALFAVLLAFWTTTLEVAADAWRIELAPTQDEQGPVAAANLW
                     GYRTAMVAAGSGALLVADPAGNAAPFLAGIGIEAGWTTAYLLIALGAFLPLPVLAAMP
                     PDPGRGGRRWAALAMGLAASAAILVAAAALTAAAGWLLLGAAGGVGIGSETNVTPYVL
                     AVCMVPFLIMAAALPRIRRAPPDSAIRRSAAIGPYVDFFWRYGFGALVVLAFVSIYRM
                     GDVLALNLSKPMIKALGYSLDQIGYADSFVALIASIVGVGLGGLMVVKWRIGIALAVG
                     ALFAAFGNFGFVWLAHRPVDDLMLYLATAADQFGNGMAGTVFVVYLSMLVNPRFPGAQ
                     YAFLSGFAFLLPRLLSGAGGAMVKQFDEAGFNGFDLFFLLSGVLSLAAILFLPLLARI
                     EPRPDDR"
     gene            4601..7681
                     /locus_tag="VF547_04340"
     CDS             4601..7681
                     /locus_tag="VF547_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8262084.1"
                     /translation="MARSFKAFLVSGTAFAALAGAAPGTAFAQVPDDEEATQPAATPT
                     GAETPDTDADLATGQPAENEILITGSRIKQNPNNSALPLQIITNEEIQRNGINSPEQL
                     LMHLPTNATGPDNLASNADVVSGAQRGTNGLSSANLRGQGNAATLVLLNGRRVAAHGL
                     SGSAVDVNQIPFSAMDRIEILKDGASAIYGTDAVGGVINFITRKDYEGIGLQGFTDIT
                     QRGDANIYRVSAIAGYGDLDDDGFNVMGAVSYSWNQILRGSDRDFVNGNQPNRGLSID
                     TRGTPIATVFNIGANANQTPNGTLLTGLTLTLPNGANGAAGGINILDLPGGAGCASVD
                     GGLPYDEVLWNLPTAQYACSWDTGRAAVIQQPIQTLTYYGRGVLRLGAEHEVSLEVTG
                     SDADSAKSFSPNQYSASATTLPWAYPRNALTAATYDQVFNSIRAAFDVPTNPNRAAQV
                     AALNARYGRPIAGRWRCIVCGPRQYVTNAKTFRAGLGVEGPLFAGWDYRAGASYAKSE
                     AASVLGTGYHYRGVQLSNNPATPFNDVGAYDTRAPTAPGAAGPGIIGLINAGILNPFS
                     LVQTPEALAGLEAVSAEGVTLYGGRYEVKQFDGSVSGPLFEIWGGDVQLAVGVDYRRE
                     TYEFNGSAAAAADQPDIFNVAFDNINALTPKKRTVKAAYAEMLIPILDNLDITLAGRI
                     DDYTGFGTTKNPKVSVKYRPFDQLLFRASYNTSFRVPTFNQIFNGTTTSPSATGANIV
                     DPFTCPSLIVSTVPGSPCANITPDTLTGGNPTLGPETAKQFNVGIVLTPMRRFSASVD
                     WWSIAVDNTITELTTLQLLENAQLFPERFLRDAPGGPIVAIDRRWINAGARRTQGLEI
                     ALRGAVDGFGGTFNAGLDGTYLLKKKEKLTQTAPYSPSLIGVFTYTGDLGLRWKHNAF
                     INYTNDDWTISLTQIFRNGYKNQALPSLSNPISATRPDYNPRVSNYVIYHFAISYLGL
                     GPDYKLTLGVKNLFDTDPPFAITYESNSGAGSSWEPRVADPRGRSFTVAAEVKF"
     gene            7731..9011
                     /gene="lysA"
                     /locus_tag="VF547_04345"
     CDS             7731..9011
                     /gene="lysA"
                     /locus_tag="VF547_04345"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX8262085.1"
                     /translation="MTGFRLLGGVLHCEDVSLATLAREVGTPVYVYSAATMLGQARSL
                     KSALAPLADPLVAYAVKANPNPAVVATLAGEGLGADVVSAGEYRCARAAGIPAERIVF
                     SGVGKTEAEMKLALEGGLCQFNLESVPEAEMLSDVALSMGRKAPVALRVNPDVEAGSH
                     AKISTGAAHNKFGVAIADAVAACARISELPGLRLRGVAVHIGSQLTSLAPLESAFERL
                     GGLIRELRSAGHAIRTADLGGGLGLVYDPAAAPPPTPADYGAMVARVTAGWDVRLIFE
                     PGRLLVGDSGVLLTSVIRIKPGGAQPFVIVDAAMNDLMRPTLYDAWHGIEAVVPGGGE
                     MIADIVGPVCETGDTFGFARTIDRVEAGDLMVIRTAGAYAATMAGTYNSRPLAPEVLV
                     DGANWAVVRERQSVEALMAGCPLPPWLESRSVRR"
     gene            complement(9034..10857)
                     /locus_tag="VF547_04350"
     CDS             complement(9034..10857)
                     /locus_tag="VF547_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262086.1"
                     /translation="MNDDVATVLAWLEKTDANRRPRRSDLKGSAGSLAVHLRHHGSIE
                     LAEEMARLDREGEGADVGLNAELALAEALAEYVARNPSHARFEAVDLLLGTGPGPWRR
                     FLDKAAEALRKNPSSLASLRERLEAEPALLIEAPAQVALASPIDFRALAEHHRLHAAA
                     RDMWGMSGWGGLHMLSALEHKLMRRADPQAWLRIVERFQFPEPVFDLVGGADFDGMEE
                     IAIFVAAAAPAYREDGTWRSDRMTVFALIAAGVQVLRRHAGLGPAGEPPDAAALDRDL
                     SLLLDSAAGRPDFQWLGHAWLQQLAWQDRSWRAWRGRPGGAVGEPLSKIMSAIAARLE
                     PLQHPVAWTRAEEPLWRLDRLVAAMLPAVVRGHGGGRLDEVVAEAIELDLVPRNGLSD
                     SVSRGGSAFVRAMSAALDCAPEPASWLDEVWRKSFPRHDRLRSWRGREAAEGHDPGAL
                     AVAAACAWLIRFGDAPGRLRQSRRLWDRLAGAVTETWLTRAEPRDPLWMICARWLAIR
                     FPSLHEGSEEASARLGKLLRALRSPARPFLDMLVDLRAGDVALADLDAALDGYTVSAL
                     SDRAVDDARRRNPVSFDDKQELAELERLRATLMEGAGPAGV"
     gene            complement(10854..12509)
                     /locus_tag="VF547_04355"
     CDS             complement(10854..12509)
                     /locus_tag="VF547_04355"
                     /inference="COORDINATES: protein motif:HMM:NF040700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPA1262 family N-terminal domain-containing
                     protein"
                     /protein_id="HEX8262087.1"
                     /translation="MPDLGLDYAQADYCGAAIRLATVVEDKVQRLLFACVELYPNEIS
                     VPPKTGEQRHSGTLTLRHSITPASVAEAFLWYEQAASGVPGVPGMPNIRLAPATLAPE
                     PGWGRWLVGADPPFEPRWHGGARLHRLVNMVEPAESVGRLGSPGDNRERWEANRSWLA
                     ERLHFDLLASDDWIGSCALLAPNPVARGLTQRLLERSPTGSETFRLAADLRDGASPAG
                     LTIRAVERRGGGLAQVSTHALDRFGSVEIRFPQPVDRLGYELHCDQRGLLASCPPAPL
                     LRQILTNMSLRRGELQIDVPSPRKSGAPARYSRPIVEPVDTDVIGNAAESPALKRLRL
                     LIERRERRTGSARPGGEHGGSDMLLFDCDREAATAAIRQLIGRARRTVTLVDPYFDEL
                     VLPEFALAAPSTNIEIRVLTNYQAKRRDLDSAMVPMERLYLALRDAADLLARHRLGKL
                     DVRVTGGKVRRYHDRFLIVDEEAWHCGHSFNQVGRIELSAMTRLRRPEAVIAMIEDDF
                     MSAEPFSDAHLRWLANEPLPLPRWRVKAARLVRSVSQWLEQRS"
     gene            complement(12579..12655)
                     /locus_tag="VF547_04360"
     tRNA            complement(12579..12655)
                     /locus_tag="VF547_04360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12619..12621),aa:Pro,seq:cgg)
     gene            complement(12861..14162)
                     /locus_tag="VF547_04365"
     CDS             complement(12861..14162)
                     /locus_tag="VF547_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8262088.1"
                     /translation="MRHVAIVGSGPAGYYTAEALQKLYGESVAIDMIDRLPVPYGLIR
                     SGVAPDHQSIKAVYKRYEKVALSANVRFVGNVCIGPDVGVEELQGLYDAVVLATGAPQ
                     DRPLDIPGSDLPGVVGSAAFVGWYNGHPDFADLDPPLDSAGAAVIGNGNVALDVARIL
                     AKTRAEFAGSDIVARALDALEASAVRAIHVLGRRGPHQIAMTPQELGELGHLEAAAPR
                     VDLADFPPAQDDALLEPGMRKSVTHLREFAALPQDKAKTIDFDFFAMPVRVEGEGRVE
                     RLIVERTRLTDDLRSVGTGETYAVECGLVVSCIGYRTPPIEGVPYEHGRGRFANSDGV
                     IADGLYCVGWARRGPTGTIGTNRPDGFEVAERIFAAFPPDGGGRSGGAGLDTLLNARG
                     VEPVAFRDWQRIEAAETGRAREGSPREKFTSIAEMIAALGR"
     gene            14267..14626
                     /locus_tag="VF547_04370"
     CDS             14267..14626
                     /locus_tag="VF547_04370"
                     /inference="COORDINATES: protein motif:HMM:NF039797.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEX8262089.1"
                     /translation="MKALLPLLAAAAAAPAAAVPPLPERQEASIPFVSQPRSIRSFRA
                     VGNDLVYLQDRRGRWYRAELGGPCIGLSWANVIGYDAGAGLSLARGDSILVEGQRCMI
                     TSLTRSESPPRKRKGKA"
     gene            complement(14747..15064)
                     /locus_tag="VF547_04375"
     CDS             complement(14747..15064)
                     /locus_tag="VF547_04375"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX8262090.1"
                     /translation="MSNPLTVDLPHKLGAEEARRRIERNLGKLTDHIPGGARVGSRWT
                     GDRLDLDIDAMGQQVAAQIDIHESLVRLTMTLPPALAFFSGMLEPLIRKQGAQLLEDR
                     SKG"
     gene            15242..18028
                     /locus_tag="VF547_04380"
     CDS             15242..18028
                     /locus_tag="VF547_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8262091.1"
                     /translation="MVRTTRVALLTTLSSLALASGPAFAQDSDPAAPADTAAEAPAGN
                     DETIVITGTRRTDRTVADSPVPVDVIGADQIVNSGQTETNKILNQLVPSFNFPQPSIA
                     DGSDALRPATLRGLSPDQTLVLINGKRRHVSALLNINGTVGRGSAAVDMNLIPGLAIG
                     RVEVLRDGAAAQYGSDAIAGVINIQLKNADHGGRASVTWGKYVTTIDNVANVTGLTLN
                     ASGQPQLDPADNRYLLANVDGEREARDGRQLTVGVNVGIPMGPNGYFNITGEIRDRDR
                     TNRAGFDLRPNYNRPAPPAFDPREVTFNRLQFRFGDPDTRDYNLFANAAVDLGGDFEL
                     YGFASYGHRDTVSAANWRQGGSVQNRDFSAIAPNVTPNAANFVPLTPDGFLPLIDSDL
                     DDFGGTVGLRWAMGGWKSDLSFGHGQNSFDYTVRDTLNTSFGPQSQRVFDAGGLRYAQ
                     DIVNLDFSKEFAIGFAKPLSVAAGAEYRSENFKIRPGDAQSWQTGPFFRASITTTAAN
                     CTTQQGVFNATTGVCSFPGRAAPAGAQGFPGIPATSATDEGRHSWAAYVELDTDPIEG
                     VTTTIAGRYEHFSDFGSTWNGKFAARWEPVEGFALRGSVSNGFRAPSLHQQFFTTTST
                     NFIAGLPVDISTLAVTSPVALALGSKPLEPEKSFNVSLGATANPLRGLTITADLYQIK
                     IDDRIVLTENLGAAGSGTAAQNAAVKAILDANGFQSVGAARFFINGLDTRTRGLDIVA
                     VYRTRMAGFGNWSLSAAYNYNKTRIDERLNALGPLATIPGLVLFGRVEGIRFTHGQPR
                     DKIVFSADGDIGRFGITARTTRYGKVVSPGAAAPLAPNALSLTAFGPDDIFLKAKWIT
                     DLEFRYKIGGRFELAVGANNLFDVYPTRSPFGPRPAAIGGVYPANQEYIPFSIFSPFG
                     FNGRFLYGRVSLEF"
     gene            18250..18759
                     /gene="msrB"
                     /locus_tag="VF547_04385"
     CDS             18250..18759
                     /gene="msrB"
                     /locus_tag="VF547_04385"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8262092.1"
                     /translation="MSDSRLSRRTFLGSAAALAVAAGAGFVLFGRNGGVAEAAERFEV
                     TRTPAEWRRILGPQRYAVLREAHTERAGSSPLDREKRRGTFLCAGCGLPVYSSAAKFD
                     SGTGWPSFTRPLPNAVRTRPDRTLFVVRTEVHCRRCGGHLGHVFDDGPPPTGQRWCMN
                     GLALKFRPA"
     gene            18767..19387
                     /gene="msrA"
                     /locus_tag="VF547_04390"
     CDS             18767..19387
                     /gene="msrA"
                     /locus_tag="VF547_04390"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8262093.1"
                     /translation="MRRLLLLLLAAAGALLLLLRPGAPEAAPPARKLAAAVFAGGCFW
                     CTEADFDKMPGVVSTTSGYSGGRVADPTYEQVSAGGTGHIEVVRVVYDPSRISYQALV
                     ARFFRTVDPLDGGGQFCDRGSQYRPAIFVANPGERRIAEATKARAAALLKKPVATLIL
                     PAAPFYPAETYHQDYYRKNPVPYRYYRYRCARDARLAAIWGKTAGH"
     gene            19633..20871
                     /locus_tag="VF547_04395"
     CDS             19633..20871
                     /locus_tag="VF547_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEX8262094.1"
                     /translation="MRALSWLRNLISSERGNVLVVGAAAMPLLIGSAAIAIDTIQVSV
                     WKRQLQRAADSSAIAGAYALSLGDDEHDAVHRDLDKNSFPVLSREEAIAIGPRLGFDR
                     TVRVTLTATRTPPFMSIFTNAPQTLVADATAALVDDGTFCMVSLYDGTDAGIDANGGA
                     EVDLTCGMKANCTGTDCVQAGGNSWIKALPIATVGGLKGSSNNFIQPTKLQPYSAAQK
                     DPFAYLPEPAPDPAECTRNGTLTETTDFSTLGNNPCFLAADIQPNTTLNVPDAVQSIT
                     VYGGNIDFKGNVTANGITWIMTGPAGAAGDLIINAQANLVMSSSDGGTFDGVLFYRDR
                     RASNIEIKINGGATSKLTGALYFPTSDITFAGNAGMNVECLQMVGQKLKFRGGAKITN
                     VCPSGSGASAFQQTVVRLVG"
     gene            20868..21317
                     /locus_tag="VF547_04400"
     CDS             20868..21317
                     /locus_tag="VF547_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8262095.1"
                     /translation="MRRLLPSLARDERGTSLIEFGFLVPFLAVLAMGIIDLSRGLAER
                     FALQQAVNRGLELVQARPAVAGATATDLDYNFALVEAQAAAGAGATVTMTRWLECNGV
                     DKGAANGSCADGEDTARYLRLRIVKNFQGEFYYSTIRMTATGAVRTQ"
     gene            21317..>21655
                     /locus_tag="VF547_04405"
     CDS             21317..>21655
                     /locus_tag="VF547_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX8262096.1"
                     /translation="MLKRLFPRGEKGIGAVEFALIAPVLIGFIVGVSQVGRMFYANAD
                     MKNASAHAARLASIYRTGGTVDDATLIAAVKARLLRDGAASKVTVTVTRGTDNGNPYV
                     ELKTDYSVPLE"
BASE COUNT         3397 a         7910 c         7174 g         3174 t
ORIGIN      
        1 gaagggtctg aatgatcccc gccttattgc cccggctcgc tttgcctcta gggtcggcgc
       61 gatgacccga ccgtcgacag ccctgcgcgc cgccgccctg ctcttggtcc tggccgggcc
      121 ttcggccgcg gcggccgcgc cctccgagcc tctccagcag cgcgtcgagg cgaagctggg
      181 cgaggccggc cccggcgtgc gcttcggact cgtcgtcgcc gacgcggcgg ggcgggagct
      241 ggtcgcggtc gcgcccgagg gccgcttcat ccccggctcg aacatcaaga tgttcacgac
      301 cgcggccgcc ttcgccacct tgccttcgct cgacgcgccg gactcggcgg ggggcgcggc
      361 ggttcggctg gagccggccg gccgcggcct cgccgacgtc gtgctcgagg gccggggcga
      421 cgcccgcctg tcgagcgcgg ccggctgcgt gcaggactgc ctctcggcgc tcgccgacgc
      481 ggtcgcggcg cggacccggc gggtgcgcga cgtgatcggc gacgacagcc tcttcccgga
      541 tcagcgctgg agcccgggga tgagctggaa caacatcccc acccggtccg gcacggccgc
      601 ctcggcgctc agcctcgacg acaacgagct gccgcttcgc gtcttcgcga ccacgccggg
      661 agcgccgccc cggctcgagc tgctcgatta ttacgaggtc cacaaccggg ccgtgaccgt
      721 cgccgccggt ccgaccgagc tcagcttcga ccggtttccg ggaagccgcc tcgtccggct
      781 cgagggcagg atcgcggccg gggccgagcc ggagtggctt cgcctcggca tcgacgatcc
      841 cgcccattat gccgcctggc ggctcaaggc gctgctggag gagcggggcg tccgggtctc
      901 cgggacggtc gcggtccgtc atcgcccgct tcgcccggcg gacgatccgg cggtgcgcaa
      961 cggcgccccg gccgctcggc cgccggaagc ggcgacggtg ctcgcgcgcc tcgttccgcc
     1021 gccgctcgcc gaggacctcg ccgcgatcag caaggccagc cagaacctcc attcggagct
     1081 cctgctccgc cggatcggcc ggcagcaggg gagcgggtcg atcgccgacg ggctcgccgc
     1141 ggtccacgcg atgctcgccg ccgccggggt gccgcgcacc ggctacgacg tctccgacgg
     1201 ctcgggcatg tcgagctaca accggatcgc cccgcgcgcg atggtcgcct tcctgcgctg
     1261 ggccgcggcc cagccctggg gcgcggcgtg gcgggcgacg ctgccgatcg gcggagtcga
     1321 cggcaccctc gacgggcgct tcaagggaag cccgctccag ggccggatct tcgccaagac
     1381 cgggactctc aacgccagca acgccctctc cggattcatg atcgcgagga gcggccgcac
     1441 tttgctcttc tccgccttcg ccaacgacgt ccccggcgga gtggccgcga ccgcggcgat
     1501 ggactcggcg ctggtcctga tcgcggagga gaattgaccc tctccctgca ggggagaggg
     1561 accggcgagc gaagctcgcc agggtgaggg cacggcctag ctcgtgacgg ccctcaccct
     1621 cccaccgcct tcggcagcgg gcccctcctc tcccctgcag ggggaggggc tttatccgtc
     1681 ccacgcccct atcacggcat tgcccgtggc tcggcggagc gcgacgatcc cggtctcgcg
     1741 gtggcgggtg ggatggacgc ggttggtgag cagggtccag gcgaggcctc gctcgaaatc
     1801 gacccacagg ccggtgccgg tgaagccggt atggccgatc gtcgcgggcg agcaggaatc
     1861 gccgccgtgc cagccgtcga agcgccgctc ccatccgcag gtgcggtcgt cgcgacagcg
     1921 cgtccggatc gcctcgagca tcgccggcga ggcgccgctg ccgtcgagca ggcccagcgc
     1981 gaagtcgagg actccggcca ccgtgccgaa cagcccggcg tgccccgcct cgccgcccag
     2041 ggcggcggca ttctcgtcat ggacctcgcc ggccaggact cgcccgcgcc aggcgcagcg
     2101 ctcggtggcg acggccggcc ccggcggcgg gccgtagccg agcccggcgc cgagcggcca
     2161 ggccgacagc ggcgcgcccg tgatccgctc gaccgcgatc ccgagcagga tgaagttgat
     2221 gtcggaatag accggcgggc cgcggcgcca ttcgcgctgc aggacgaagg cccgaagccg
     2281 cgccgggtcg tcgccatagg tgtagatcgg ctcggtcgcc gggaggaagg tccggtgggc
     2341 gaggcagtcg cggaaggtca gccgccgctc ggcggccccg tccggatcat attggcgcag
     2401 gtccgggatc gcgtcggtca gcgggcggtc gaggccgagg cggccctcgt cggccaggcg
     2461 caggatcatc gtcgttgtcg cgatcacctt ggtcagcgag gcgaggtcga accaatggtc
     2521 ctcgctcagc gcctccggcg ccggctccag cgcggccatg ccggagacct tcaccgatcg
     2581 gcggccgtcg gagcggacga tcccgagcgc cgcgcccggg atccgaccct ccgcgaccgc
     2641 cgcggcggcc ggagcgaagg ccgcctcgat catcggtcgt ccggcctcgg ctcgatccgg
     2701 gcgagcagcg gcaggaacag gatcgcggcg aggctgagca cgccggaaag aaggaagaag
     2761 aggtcgaagc cgttgaagcc ggcctcgtcg aactgcttga ccatcgctcc gccggccccc
     2821 gagagaagcc tcggcagaag gaaggcgaag cccgacagga aggcatattg ggcgcccggg
     2881 aaacgcgggt tcaccagcat cgagagatag acgacgaaaa ccgtcccggc catgccgttg
     2941 ccgaactggt cggcggcggt ggcgaggtag agcatcaggt cgtcgaccgg ccggtgggcg
     3001 agccagacga agccgaaatt gccgaacgcg gcgaacaggg cgccgacggc gagggcgatt
     3061 ccaatgcgcc acttcacgac catcaggccc ccgagcccga cgccgacgat gctcgcgatc
     3121 agggcgacga agctgtcggc atagccgatc tggtcgagcg aatagcccag cgccttgatc
     3181 atcggcttgg acaggttgag cgcaagcacg tcgcccatcc ggtagatgga gacgaaggcc
     3241 aggacgacga gcgccccgaa gccgtagcgc cagaagaaat cgacataggg gccgatcgcc
     3301 gccgaccggc ggatcgcgga gtcgggcggc gcccgccgga tccggggcag ggcggcggcc
     3361 atgatcagga acgggaccat gcacaccgcc agcacatagg gggtgacgtt ggtctcgctg
     3421 ccgatgccga cgccgcccgc ggcgcccagc agcagccagc cggccgccgc ggtcagcgcg
     3481 gccgcggcga cgaggatggc ggcgctcgcc gcgagcccca tcgccagcgc cgcccagcgc
     3541 cggccgccgc ggccgggatc gggcggcatc gccgccagca ccggcagcgg caggaaggcg
     3601 cccagcgcga tcagcagata ggcggtggtc cagcccgcct cgatgccgat cccggccagg
     3661 aagggcgcgg cgttgccggc cggatcggcg acgagcaggg cgccgctgcc cgcggcgacc
     3721 atcgcggtcc ggtagcccca cagattggcg gcggcgaccg ggccctgctc gtcctgggtc
     3781 ggggcgagct cgattcgcca ggcgtcggcg gcgacctcga gcgtcgtcgt ccagaaggcg
     3841 agcagcacgg cgaacagggc ggtcacggct aggttcgagt ccgacgaggt cagcgccatc
     3901 gccaccatcg aggtgaagat gccgagctgc gagagcatga tccagccgcg cctcttgccc
     3961 cagaagcgcg cgaagccggg cacgtcgaag cggtcgagcg ccggcgccca caggaacttg
     4021 aaggtgggca gcagctgcac ccaggtgaag aagccgatca ccaccagcac gacgccgtgc
     4081 gactgaagcc tcagcgccag caccgtggag aacatgtaga agggcaggcc ggcggagaag
     4141 ccgagcagga cgtagagcgc catccgcccg agccgggacc gaaccaccgg cggcggaatg
     4201 gccgcggctt cagccaaggc ggtcgggtcg ggaagcgggc cgcatggccc ccaagcttca
     4261 ccggcgcgga cgaacagtca atgtcggcga agccaaaatc cgtctcccct ccttcacggg
     4321 cgaggagcga gcgcggtggc ttcccggcaa aggcgggacc cagctcgtcc tcggctctgg
     4381 accccggcct cggccgggga tctgccggcc cgggtcgagc cttcgccttg ggccttctcg
     4441 cgcagaaatg ggaaagggat ggcgggcggc gctgttgcgt tcgggccacg ctcggcgggg
     4501 gagtgcttcg aggttatggc atcgccaaca gaaactatgg cgcctgatgg cggtcctgtt
     4561 acttaatggc ggcgcaacgg gctttcgggg ggatgtcgac atggccaggt cgttcaaagc
     4621 tttcttggtt tcgggaacgg ccttcgcggc tctggcgggc gcggcgcccg gcacggcgtt
     4681 cgcccaggtg ccggacgacg aggaagccac ccaaccggcg gcgaccccca ccggcgccga
     4741 gacgcccgac acggacgccg acctcgcgac cggccagccg gccgagaacg agatcctgat
     4801 caccggctcg cggatcaagc agaacccgaa caacagcgcg ctgccgctgc agatcatcac
     4861 caacgaggag atccagcgca acggcatcaa cagcccggag cagctgctga tgcacctgcc
     4921 caccaacgcc accggcccgg acaatctcgc gtccaacgcc gacgtcgtct ccggagccca
     4981 gcgcggcacc aacggccttt cctcggcgaa cctgcgaggc cagggcaatg cggcgaccct
     5041 cgtgctcctc aacgggcgcc gggtcgccgc ccacggcctc agcggctcgg cggtcgacgt
     5101 caaccagatc ccgttcagcg cgatggaccg gatcgagatc ctgaaggacg gcgcctcggc
     5161 catctacggc accgacgcgg tcggcggcgt gatcaacttc atcacccgca aggattatga
     5221 agggatcggc ctgcagggct tcaccgacat cacccagcgg ggcgacgcca acatctaccg
     5281 ggtctcggcg atcgccggct acggcgacct cgacgacgac ggcttcaacg tgatgggcgc
     5341 cgtctcctac agctggaacc agatcttgcg cggcagcgac cgcgacttcg tcaacggcaa
     5401 ccagcccaac cggggcctgt cgatcgacac gcgcggaact ccgatcgcca ccgtgttcaa
     5461 catcggcgcc aatgccaacc agacgccgaa cggcactctg ctcaccggcc tgaccctgac
     5521 ccttccgaac ggcgcgaacg gcgccgccgg cggaatcaac atcctcgacc tgcccggcgg
     5581 cgccggctgc gcttcggtcg acggcggcct gccctatgac gaagtgctgt ggaacctgcc
     5641 caccgcgcaa tatgcctgct cctgggacac cggccgcgcg gcggtgatcc agcagccgat
     5701 ccagacgctc acctattatg gccggggcgt cctgaggctg ggcgcggagc atgaggtctc
     5761 gctcgaggtc accgggtcgg atgcggattc ggccaagagc ttctcgccca accagtacag
     5821 cgccagcgcc acgaccctgc cctgggccta tccccgcaac gcgctgaccg cggcgaccta
     5881 cgaccaggtg ttcaactcga tccgtgccgc gttcgacgtg ccgaccaacc ccaaccgcgc
     5941 cgcgcaggtc gcggcgctga atgcgcgcta cggccgtccg atcgccggcc gctggcggtg
     6001 catcgtctgc gggccgcgtc agtatgtcac caatgccaag accttccggg ccgggctggg
     6061 cgtcgaaggc ccgctgttcg caggctggga ctatcgcgcg ggcgcctcct acgcgaagag
     6121 cgaagccgcc tcggtgctcg gcaccggcta tcattaccgc ggcgtccagc tcagcaacaa
     6181 tccggcgacg ccgttcaacg acgtcggcgc ctacgacacc cgggcgccga ccgctccggg
     6241 cgccgccggc cccgggatca tcggcctgat caacgccggc atcctcaacc ccttctccct
     6301 cgtccagacg cccgaggcgc tggccgggct ggaggcggtg tcggccgagg gcgtcacgct
     6361 ctacggcggc cgctacgagg tcaagcagtt cgacggctcg gtgtcgggcc cgctgttcga
     6421 gatctggggc ggagacgtcc agctcgccgt cggggtcgac tatcgccgcg agacctatga
     6481 gttcaacggc tcggcggcgg ccgcggcgga ccagcccgac atcttcaacg tcgcgttcga
     6541 caacatcaac gcgctcacgc ccaagaagcg caccgtcaag gcggcctatg ccgagatgct
     6601 gatcccgatc ctggacaatc tcgacatcac cctggcgggc cggatcgacg actataccgg
     6661 cttcggcacg accaagaatc cgaaggtctc ggtcaagtac cggccgttcg atcagctgct
     6721 gttccgagcc tcgtacaaca ccagcttccg ggttccgacc ttcaaccaga tcttcaacgg
     6781 cacgaccacc tcgccctcgg cgaccggagc gaacatcgtc gatccgttca cctgcccgag
     6841 cctgatcgtg agcacggtcc ccggatcgcc ctgcgccaac atcactccgg acacgctgac
     6901 cggcggcaat ccgaccctcg ggccggagac ggcgaagcag ttcaacgtcg ggatcgtcct
     6961 cacgccgatg cggcgcttca gcgcgtcggt cgactggtgg tcgatcgcgg tcgacaacac
     7021 gatcaccgag ctgaccacgc tgcagctgct ggagaatgcc cagctgttcc ccgagcgctt
     7081 cctccgcgac gctccgggcg gcccgatcgt cgccatcgac cggcgctgga tcaacgccgg
     7141 cgcccgccgc acccagggcc tcgaaatcgc cctgcgcggc gccgtcgacg gcttcggcgg
     7201 aacgttcaac gcgggcctcg acggcaccta tctgctgaag aagaaggaga agctcaccca
     7261 gacggcgccc tacagcccga gcctgatcgg agtcttcacc tacacgggcg acctcggcct
     7321 ccggtggaag cacaatgcgt tcatcaacta caccaacgac gactggacca tctcgctgac
     7381 gcagatcttc cgcaacggct acaagaacca ggccctgccg agcctctcga acccgatcag
     7441 cgcgacccgg ccggactaca acccccgggt cagcaactat gtgatctacc atttcgcgat
     7501 cagctatctg gggcttgggc cggactacaa gctgaccctc ggcgtcaaga atctgttcga
     7561 caccgatccg cccttcgcga tcacctatga gagcaactcg ggcgccggca gctcctggga
     7621 gccgcgcgtc gccgatcccc gcggccgatc gttcaccgtc gccgccgagg tcaagttctg
     7681 acgcccgatc ccctctcccg ttttgcgcgg gagaggctag gggaagtcga atgacgggat
     7741 ttcgattgct gggcggcgtg ctccattgcg aggacgtgtc gctggcgacc ctcgcgcgcg
     7801 aggtcggaac gccggtctac gtctattccg ccgcgacgat gctgggacag gcgcggagtc
     7861 tgaagtcggc gctggccccg ctcgccgatc cgctcgtcgc ctatgccgtc aaggccaacc
     7921 ccaacccggc ggtggtcgcg accctcgccg gtgaggggct cggcgccgac gtcgtctcgg
     7981 ccggcgaata tcgctgcgcg cgcgccgccg gaatccccgc cgagcggatc gtcttttcgg
     8041 gcgtcggcaa gaccgaagcg gagatgaagc tcgccctcga aggcggcctc tgccagttca
     8101 acctcgaatc ggtcccggaa gccgagatgc tgtcggacgt ggcgctgtcg atggggcgga
     8161 aggcgccggt cgccctgcgc gtcaatcccg acgtcgaggc gggctcgcac gccaagatct
     8221 cgaccggcgc cgcccacaac aagttcggag tcgcgatcgc cgacgccgtc gccgcctgcg
     8281 cgcgcatcag cgagctgccc ggcctcaggc ttcgcggagt cgccgtccat atcggcagcc
     8341 agctgaccag cctcgctccg ctcgagtccg ccttcgagcg gctcggtggg ctgatccggg
     8401 agctccgctc ggccggccac gcgatccgaa cggcggatct cggcggcggg ctcggcctgg
     8461 tctacgatcc ggccgcggcc ccgccgccga ctccggccga ctatggcgcg atggtcgccc
     8521 gggtgacggc gggctgggac gtccggctga tcttcgagcc cggccggctg ctggtcggcg
     8581 attccggagt gctcctgacg agcgtgatcc ggatcaagcc cggcggggcg caacctttcg
     8641 tgatcgtcga cgcggcgatg aacgacctga tgcggcccac tctctacgac gcctggcacg
     8701 gcatagaggc cgtcgtcccg ggcggggggg agatgatcgc cgacatcgtc ggtccggtct
     8761 gcgagaccgg cgatacgttc gggttcgccc ggacgatcga ccgggtcgag gccggcgacc
     8821 tgatggtcat ccgaaccgct ggcgcctatg ccgcgaccat ggccggcacc tacaattcgc
     8881 gcccgctcgc ccccgaagtt ctggtcgatg gcgccaactg ggccgtcgtc cgcgagcggc
     8941 agagcgtgga ggccttgatg gcgggctgcc cgctgccgcc ctggctggag tcacgatcgg
     9001 ttcgtcgttg agggctcgcc gccgccggcg gagtcaaacg ccggccggcc ccgcgccttc
     9061 catcagcgtc gcgcgaagcc gttcgagttc ggcgagctcc tgcttgtcat cgaaggaaac
     9121 gggatttcgg cgcctggcgt cgtcgaccgc gcggtcgctc agcgcactga cggtgtagcc
     9181 gtccagcgcg gcatccagat cggccagggc gacgtccccg gcacgcaggt cgacgagcat
     9241 atcgaggaag ggccgggccg gcgagcgcag cgcgcggagc agcttgccga gccgggccga
     9301 agcctcctcg ctcccctcgt gcaggctcgg aaagcggatg gccagccatc gggcgcaaat
     9361 catccagagc gggtcgcgcg gctcggcgcg ggtcagccag gtctcggtca ccgcgccggc
     9421 gagcctgtcc cacagcctgc gggattggcg gagccggccg ggcgcgtcgc cgaacctgat
     9481 cagccaggca caggcggcgg ccaccgcaag cgctcccgga tcgtggccct ccgcggcttc
     9541 ccggcctctc cagctccgca gccgatcgtg ccttgggaag ctctttcgcc agacctcatc
     9601 gagccaggat gcgggttccg gcgcgcaatc cagggcggcg ctcatcgccc ggacgaaggc
     9661 gctcccgccg cggctgaccg aatcggagag tccatttcgc ggcacgaggt caagctcgat
     9721 cgcctccgcg accacctcgt cgaggcgacc gccgccgtga ccgcggacga ccgcgggcag
     9781 catcgcggcg accaggcggt ccaagcgcca cagcggctcc tccgcccggg tccaggccac
     9841 cgggtgctgg agaggctcca gcctcgcggc gatcgccgac atgatcttcg agagcggctc
     9901 gccgaccgcg ccgcccggcc gcccgcgcca ggcgcgccag ctgcggtcct gccacgcaag
     9961 ctgctgcagc catgcgtgcc ccagccattg gaagtcgggc ctgcccgcgg cggagtcgag
    10021 gagaagcgac aaatcccgat ccagggccgc ggcatcgggg ggctcaccgg ccggtcccag
    10081 gccggcgtgc cggcgaagga cttgcactcc ggccgcaatc agcgcgaaaa cggtcatccg
    10141 gtcggaccgc caggtgccgt cctcccggta agccggcgcc gcggcggcga caaagatcgc
    10201 gatttcctcc attccgtcga aatcggcgcc gccgacgaga tcgaagaccg gctccgggaa
    10261 ctggaaccgc tccacgattc gcagccaggc ctgcggatcg gcccgtctca tcagcttgtg
    10321 ctcgagggcc gaaagcatgt gcagcccgcc ccagccggac atgccccaca tgtcgcgggc
    10381 agccgcatgc agccggtggt gctcggccag ggcgcggaaa tcgatcgggc ttgcaagcgc
    10441 cacttgagcc ggcgcctcga tgagcagcgc cggctcggcc tccagccgtt cccgaagcga
    10501 cgcaaggctc gaagggttct ttctcagcgc ctcggcagcc ttgtccagga accggcgcca
    10561 cggtcccggg ccggtgccca gcagcaagtc gacggcctcg aaacgggcat ggctgggatt
    10621 gcgcgccaca tattcggcca gcgcctcggc cagcgccaat tcggcgttca gcccgacgtc
    10681 cgccccctct ccctcccggt cgagcctggc catctcttcc gcgagctcga tgcttccgtg
    10741 gtgtcgaagg tgcacggcaa gcgaacccgc cgatcccttc agatcgctgc gcctcggcct
    10801 gcggttcgca tcagtcttct cgagccaggc gagaaccgtc gccacgtcgt cgttcacgac
    10861 ctttgctcca gccattgcga aacgctccgg acaagcctgg ccgccttgac ccgccagcgc
    10921 gggagcggga gaggttcgtt ggcgagccag cgcagatggg catccgaaaa gggctcggcg
    10981 ctcatgaagt cgtcctcgat catcgcgatg acggcttcag ggcggcggag ccgggtcatg
    11041 gcgctcagct cgatccttcc gacttggttg aacgagtgcc cgcaatgcca tgcttcctcg
    11101 tcgacgatga ggaagcggtc gtggtagcgc ctaaccttgc cgccggtgac gcgcacgtcg
    11161 agcttgccga gacggtgccg tgcgagcagg tcggcggcgt cgcgcagggc gaggtacagc
    11221 ctttccatcg ggaccatggc cgaatcaaga tcccggcgct ttgcctggta gttggtcaga
    11281 acccggattt cgatattggt ggacggggcc gccagcgcga attccggcag aaccagctcg
    11341 tcgaaatagg ggtcgacgag cgtgacggtc cgcctcgccc gaccgatgag ctggcggatt
    11401 gccgccgtcg cggcttcccg gtcgcagtcg aatagcagca tgtcgcttcc gccatgctca
    11461 cccccgggcc gtgccgatcc ggttcgacgc tcgcgacgtt cgatcaatag gcggagcctc
    11521 ttcagggcag gggattcggc ggcattcccg atgacatcgg tatcgaccgg ctcgacgatc
    11581 ggccggctgt agcgcgccgg cgctcccgac ttcctgggcg acgggacgtc gatttgcagc
    11641 tcgccccgcc gaaggctcat gttggtcagg atctgtcgga gaaggggagc cggggggcag
    11701 ctggccagca ggccgcgctg gtcgcaatgc aattcgtagc ccagccggtc gaccggctgc
    11761 ggaaagcgaa tctcgacgct tccgaaccga tcgagggcgt gggtcgacac ttgcgcgagc
    11821 ccgcctcccc gcctttcgac ggccctgatg gtcaggcccg cggggcttgc gccatcccga
    11881 aggtcggcgg ccagcctgaa ggtctccgag cccgtggggg acctctcgag cagccgttgg
    11941 gtgaggcccc gggcgaccgg gttcggcgcc agcagggcgc agctcccgat ccagtcgtcg
    12001 ctggcgagca ggtcgaagtg aagccgctcg gcgagccagg accggttcgc ctcccatcgc
    12061 tcccgattgt cgcccggtga gccgagccgc ccgacgctct cggccggctc gaccatgttg
    12121 acgagccggt gcaggcgtgc tccgccgtgc cagcgcggct cgaacggcgg gtccgccccg
    12181 acgagccacc gaccccatcc gggctcaggc gccagtgtcg ccggcgccaa tctgatgttc
    12241 ggcatgcccg gtacgccggg aactccgctc gccgcctgct cataccacag gaacgcctcc
    12301 gcgacgctcg ccggcgtgat gctgtgtcgc aaggtgagcg ttcccgaatg ccgctgctca
    12361 ccggtcttgg gtggaaccga tatctcattt ggatagagct ccacgcaggc gaacagcagg
    12421 cgctgcacct tgtcctccac cactgtcgcg aggcggatcg cggctccgca ataatctgcc
    12481 tgcgcgtagt caagcccgag atcaggcatg gtgttggcct cgatttgcct gtcctcgccc
    12541 gcgggcctag ccggcacttc agcagcgtga gcgaaaaatg gtcggggaga gaggattcga
    12601 acctccggcc cctgcctccc gaagacagtg ctctaccagg ctgagctact ccccgaccgg
    12661 aaccggctcg aaggccgccc gggagacagg tggcgctcaa taagggaggc gatccgggag
    12721 cgcaagcttt gcagatcctc ccaggatttc ctggggaggg ggaccgcgcg aagcgcggtg
    12781 gaggggttct cggcgtctga gaagaacccc tccaccaggc ttcgccttgt ccccctcccc
    12841 gcgaaatcgc ggggaggatc tcagcggccg agcgcggcga tcatctcggc gatcgaggtg
    12901 aacttctcgc gggggctgcc ctcgcgggcg cggccggtct cggcggcctc gatccgctgc
    12961 cagtcgcgga aggcgaccgg ctcgacgccg cgggcgttga gcagggtgtc gagcccggcc
    13021 ccgccgctgc ggccgccgcc gtccgggggg aaggctgcga agatccgctc ggcgacctcg
    13081 aagccgtcgg gccggttggt gccgatcgtc ccggtcggcc cgcgccgcgc ccagccgacg
    13141 caatagaggc cgtcggcgat caccccgtcc gaattggcga agcggccgcg cccgtgctcg
    13201 taggggacgc cttcgatggg gggcgtgcgg tagccgatgc agctcaccac cagcccgcac
    13261 tcgaccgcat aggtctcgcc ggtgccgacc gagcgcaggt cgtcggtgag ccgcgtccgc
    13321 tcgacgatca gccgctcgac ccggccctcg ccttccaccc gcaccggcat ggcgaagaag
    13381 tcgaagtcga tcgtcttggc cttgtcctgc ggaagcgcgg cgaactcgcg caaatgggtg
    13441 accgacttgc gcatccccgg ctcgagcagg gcgtcgtcct gcgccggcgg gaagtcggcg
    13501 aggtcgaccc gcggcgcggc cgcctcgaga tggccgagct cgcccagctc ctggggggtc
    13561 atggcgatct ggtgcggacc gcgccggccg agcacgtgga tcgcccgcac ggcgctcgcc
    13621 tcgagcgcgt cgagggcgcg cgcgacgatg tcggagccgg cgaactcggc ccgggtcttg
    13681 gcgaggatgc gggcgacgtc gagggcgacg ttgccgttgc cgatcaccgc ggccccggcc
    13741 gagtcgagcg gaggatcgag gtccgcgaaa tcgggatggc cgttatacca gccgacgaag
    13801 gcggccgacc cgacgacgcc gggcaggtcg ctcccgggaa tgtcgagcgg ccggtcctgc
    13861 ggcgctccgg tggcgagcac gacggcgtcg tacagcccct ggagctcctc gactccgacg
    13921 tccgggccga tgcagacgtt gccgacgaag cggacgttgg ccgacagggc caccttctcg
    13981 tagcgcttgt agaccgcctt gatcgactgg tggtcggggg cgactccgga gcggatcagg
    14041 ccgtagggga cgggcagccg gtcgatcatg tcgatcgcca ccgactcgcc gtagagcttc
    14101 tggagcgctt cggcggtgta atagccggcg gggccggagc cgacgatcgc cacatgccgc
    14161 atcaaacgca cctcccgccc gaggcggccg atgctagcgc cgccccggcg ggaggcaagc
    14221 cgccatcgtc atccccggtt cagagggcct gctccagggt cgcggcatga aagcgcttct
    14281 tcccctgctc gccgccgccg ccgcggcccc cgccgccgcc gtcccgcccc tcccggagcg
    14341 ccaggaggcg agcatcccgt tcgtcagcca gccgcgctcg atccgaagct tcagggcggt
    14401 cggaaacgac ctcgtctatc tccaggaccg ccgcggccgc tggtaccgcg ccgagctcgg
    14461 cgggccctgc atcggcctct cctgggccaa cgtcatcggc tacgacgccg gcgccggcct
    14521 cagcctcgcc cggggcgact cgatcctcgt cgagggccag cgctgcatga tcacgtcgct
    14581 gacccggagc gagtcgccgc cgcgcaagcg caagggcaaa gcctagctcc ttcgatacga
    14641 gccttcgcct tcgctcagtc tctactcagg atgagcggtt ggattgcaat tctctccgct
    14701 catcctgagt agccgctgag cgaagtcgaa gcggcgtaga aggacgtcag cccttgctcc
    14761 ggtcctcgag cagctgcgcg ccctgcttgc ggatcagcgg ctcgagcatg ccgctgaaga
    14821 aggccagcgc cggcgggagg gtcatggtca gccggaccag gctctcgtgg atgtcgatct
    14881 gcgcggcgac ctgctggccc atcgcgtcga tgtcgaggtc gagccggtcg ccggtccagc
    14941 gcgagccgac ccgggcgccg cccggtatgt ggtcggtcag cttgcccagg ttgcgctcga
    15001 tccggcgccg cgcctcctcg gcgccgagct tgtgggggag gtcgacggtc aggggattgc
    15061 tcatcgggcg cccttagcgg ctcgtgcccg gctccaccaa ggcttttccg tcctgtggtg
    15121 ccgcgggcac agtggcgcgg ccttttttgc attgcagcga aaaacccttt cctgccgcgg
    15181 cgattgacat aagctgcacc ttccagcgca gccgggggct gccaatcggg ggagatttca
    15241 aatggttcgc acgacgcggg tcgctctgct gacgacgctt tcctccctgg ccctggcctc
    15301 gggcccggcc ttcgcccagg actcggatcc cgccgctccg gccgacacgg ccgccgaggc
    15361 gcccgccggc aacgacgaga ccatcgtcat caccggcacc cgccgcaccg accgcaccgt
    15421 cgccgacagc ccggtgcccg tggacgtgat cggggccgac cagatcgtca attcgggcca
    15481 gaccgagacc aacaagatcc tcaaccagct ggtcccctcg ttcaacttcc cgcagccctc
    15541 gatcgccgac ggctccgacg ccctccgccc ggcgacgctg cgcggcctca gcccggacca
    15601 gacgctggtc ctgatcaacg gcaagcgccg ccacgtctcg gccctgctca acatcaacgg
    15661 gacggtcggc cgcggctcgg ccgccgtcga catgaacctg atccccggcc tcgccatcgg
    15721 ccgggtcgag gtgcttcgcg acggcgccgc cgcccagtac ggctccgacg cgatcgccgg
    15781 cgtgatcaac atccagctga agaatgccga tcatggcggc cgagcctcgg tcacctgggg
    15841 caaatatgtg acgacgatcg acaatgtcgc caacgtcacc ggcctcaccc tcaacgcctc
    15901 cggccagccg cagctcgacc cggccgacaa ccgctatctc ctcgccaatg tcgacggcga
    15961 gcgcgaggcc cgcgacggcc gccagctgac cgtcggcgtc aacgtcggca tcccgatggg
    16021 gccgaacggc tatttcaaca tcaccggcga gatccgcgac cgcgaccgca ccaaccgcgc
    16081 cggcttcgac ctcaggccca attacaaccg gcccgcgccg ccggccttcg acccgcgcga
    16141 ggtcaccttc aaccggctcc agttccgctt cggcgacccc gacacccgcg actacaacct
    16201 cttcgccaat gccgcggtcg atctcggcgg cgacttcgag ctctacggtt tcgcctccta
    16261 cggccaccgc gacacggtca gcgccgccaa ttggcgccag ggcggctcgg tccagaaccg
    16321 cgacttcagc gcgatcgctc cgaacgtcac tccgaacgcg gccaatttcg tcccgctgac
    16381 tccggacggc ttcctgccgc tgatcgattc ggatctcgac gacttcggcg gcaccgtcgg
    16441 cctcaggtgg gcgatgggcg gctggaagag cgatctctcc ttcggccacg ggcagaacag
    16501 cttcgactat acggtccgcg acacgctcaa cacctcgttc ggcccgcaga gccagcgcgt
    16561 gttcgacgcc ggcgggctcc gctacgccca ggacatcgtc aatctcgact tctcgaagga
    16621 gttcgcgatc ggcttcgcca agccgctctc ggtcgccgcc ggcgccgagt accgctccga
    16681 gaatttcaag atccgccccg gcgacgccca gtcctggcag accgggccct tcttccgcgc
    16741 ctcgatcacg acgaccgcgg ccaactgcac cacccagcag ggcgtgttca acgccacgac
    16801 cggggtgtgc agcttccccg gccgcgccgc gccggcgggc gcgcagggct tcccgggcat
    16861 ccccgccacg agcgccaccg acgagggccg ccacagctgg gcggcctatg tcgagctcga
    16921 caccgatccg atcgagggcg tcaccaccac catcgccggc cgctacgagc atttctccga
    16981 tttcggctcg acctggaacg gcaagttcgc cgcgcgctgg gagccggtcg agggcttcgc
    17041 ccttcgcggg tcggtgtcga acggcttccg cgcgccgtcg cttcaccagc aattcttcac
    17101 caccacctcg accaacttca tcgccggcct tccggtcgac atctcgaccc tggcggtgac
    17161 cagcccggtc gccctggcgc tcggctcgaa gccgctggag ccggagaagt cgttcaacgt
    17221 cagcctcggc gccaccgcca atccgctgcg cggcctcacc atcaccgccg acctctacca
    17281 gatcaagatc gacgaccgca tcgtcctcac cgagaatctg ggcgcggccg gaagcggcac
    17341 cgcggcccag aatgcggcgg tcaaggcgat cctggacgcc aacggcttcc agagcgtggg
    17401 cgccgcccgc ttcttcatca acggcctcga cacgaggacc cgcggcctcg acatcgtcgc
    17461 cgtctaccgc acccgcatgg ccgggttcgg caactggtcg ctgtcggccg cctacaatta
    17521 caacaagacc cggatcgacg agcggctcaa cgcgctcggg ccgctcgcca ccatcccggg
    17581 cctggtcctg ttcggccggg tcgagggcat ccgcttcacc cacggccagc cgcgcgacaa
    17641 gatcgtgttc agcgccgacg gcgacatcgg caggttcgga atcacggccc gcacgacccg
    17701 ctacggcaag gtcgtgtcgc cgggcgcggc ggcgccgctc gcccccaatg cgctcagcct
    17761 gaccgccttc ggccccgacg acatcttcct caaggccaag tggatcaccg atctcgagtt
    17821 ccgctacaag atcggcggcc ggttcgagct ggccgtcggc gccaacaatc tgttcgacgt
    17881 ctatccgacc cgctcgccct tcggcccccg gccggcggcg atcggcggag tctatccggc
    17941 caaccaggaa tatatcccct tctcgatctt ctcgccgttc ggcttcaacg gccgcttcct
    18001 ctacggccga gtgtcgctcg aattctaagg cccaaagatt cctccccggc attggccggg
    18061 gagggggacc aggcgaagcc tggtggaggg gtcctttcag agcccgaaga acccctccac
    18121 cgcgcttcgc gcgatcccct cccccggaaa tccgggggag gaatttccga gcctggcctg
    18181 gcctttcgtc caggggtgac gaaccatccg ctcgttccta cataagtcgc ctgtcaggaa
    18241 ggcggtccca tgtccgactc tcgtctctca cgccgcacct tcctcggctc ggccgccgca
    18301 ctcgccgtcg ccgcgggcgc cggcttcgtc ctcttcggca ggaacggcgg agtcgccgag
    18361 gccgccgagc gcttcgaggt cacccgcacg cccgccgaat ggcgccggat cctcggcccg
    18421 cagcgctacg ccgtgcttcg cgaggcgcat accgagcgcg ccggctcgag cccgctcgac
    18481 cgcgagaagc gccgcggcac cttcctgtgc gcgggctgcg gccttccggt ctacagctcc
    18541 gccgccaagt tcgacagcgg caccggctgg cccagcttca cccgcccgct ccccaatgcg
    18601 gtgcgcaccc gccccgaccg aaccctgttc gtcgtgcgca ccgaagtcca ttgccggcgc
    18661 tgcgggggcc atctcggcca cgtcttcgac gacggtccgc cgccgaccgg ccagcgctgg
    18721 tgcatgaacg gcctcgcgct caagttccgg ccggcgtgac cgggcgatga ggcgcctcct
    18781 cctcctcctg ctcgccgccg cgggcgcgct gctgctgctg cttcgcccgg gcgcgcccga
    18841 agccgcgccg ccggcgcgca agctggcggc ggccgtgttc gccggcggct gcttctggtg
    18901 caccgaagcc gatttcgaca agatgcccgg cgtcgtctcg accacctccg gctattccgg
    18961 cggccgggtc gccgatccga cctacgagca ggtctcggcc gggggcaccg gccatatcga
    19021 ggtggtccgg gtggtctacg atccgtcccg gatcagctac caggcgctcg tcgcccgatt
    19081 cttccggacc gtcgacccgc tcgacggcgg cggccagttc tgcgaccgcg gcagccagta
    19141 ccgtccggcc atcttcgtcg ccaatcccgg cgagcgccgg atcgcggagg cgaccaaggc
    19201 gcgggccgcg gccctgctca agaagccggt cgccaccctc attctgcccg ccgcgccctt
    19261 ctatccggcc gagacctacc accaggacta ttaccgcaag aaccccgtcc cctatcgcta
    19321 ttatcgctac cgctgcgccc gcgacgcccg gctggccgcg atctggggga agaccgcggg
    19381 gcattgaccc gccgtcgggg cagcgtcctt cgatatgggc cttcgccaag ctcagtccct
    19441 actcaggatg agcggttggg ctcttcgctc atcctgagta gccgctgagc gaagtcgaag
    19501 cggcgtatcg aaggacgccc cccaaatcat ggccaatttt ccggtaacca ttgcgctcaa
    19561 cacactccta accgcgaggc cctaatcgcg gtcccctcta gctgcacatt gcaccgacga
    19621 ggaccgagta agatgagggc attgagctgg cttagaaacc tcatttcgag cgagcgcggc
    19681 aacgtgctgg tggtcggggc ggcggcgatg ccgctgctga tcgggtcggc cgccatcgcc
    19741 atcgacacca tccaggtcag cgtgtggaag cgccagctgc agcgcgccgc cgattcgagc
    19801 gcgatcgccg gcgcctacgc cttgtcgctg ggcgacgacg agcatgacgc ggtccatcgc
    19861 gacctcgaca agaatagctt cccggtgctc agccgggagg aggcgatcgc gatcgggcct
    19921 cggctcggct tcgaccggac ggtgcgggtc accctcaccg ccacccggac cccgcccttc
    19981 atgtcgatct tcaccaacgc gccgcagacc ctggtcgcgg acgcgaccgc ggcgctggtc
    20041 gacgacggca ccttctgcat ggtttcgctc tacgacggca ccgacgccgg aatcgacgcc
    20101 aatggcggtg ccgaggtcga cctcacctgc ggcatgaagg ccaattgcac cggcacggac
    20161 tgcgtccagg ccggtggcaa ttcctggatc aaagccctcc ccatcgccac ggtgggcggc
    20221 ctcaagggca gctcgaacaa tttcattcag ccgactaagc tgcagcccta ttccgcggcc
    20281 cagaaggatc cgttcgccta tctgcccgaa ccggcgcctg atccggcgga atgcactcgc
    20341 aacggcactc tcaccgagac cacggatttc tccaccctcg gcaacaaccc gtgcttcctc
    20401 gccgccgaca tccagccgaa cacgacgctg aacgtccccg acgccgtcca gtcgatcacc
    20461 gtctatggcg gcaatatcga cttcaagggc aacgtcaccg ccaacggcat cacctggatc
    20521 atgactggac ccgccggcgc tgcgggcgac ctcatcatca atgcgcaggc caacctggtg
    20581 atgagctcgt cggacggcgg cacgttcgac ggcgtgctct tctaccgcga tcgccgagcc
    20641 tctaacatcg agatcaagat caacggcggc gccacctcga agctcaccgg agcgctctat
    20701 ttccccacct cggacatcac cttcgccggc aatgccggaa tgaacgtcga gtgcctgcaa
    20761 atggtcggcc agaagctcaa gtttcgcggc ggcgccaaga tcaccaacgt ctgcccctcg
    20821 ggtagcggcg cctcggcctt ccagcagacc gtcgtgaggc tggtcggatg agacggctcc
    20881 ttccctccct cgcccgcgac gagcgcggca ccagcctgat cgagttcggc ttcctggtcc
    20941 cgttcctcgc ggttctggcg atgggtatca tcgatctcag ccggggcctc gcggagcgct
    21001 tcgccctcca gcaggcggtc aatcgcgggc tcgagctggt ccaggccagg cccgccgtgg
    21061 ccggcgccac cgcgacggac ctcgactata atttcgccct cgtcgaggcc caggccgcgg
    21121 ccggcgccgg agcgacggtg acgatgaccc gctggctcga atgcaacggc gtcgacaagg
    21181 gcgccgccaa cggcagctgc gccgacggcg aggataccgc ccgctacctg aggctccgga
    21241 tcgtcaagaa tttccagggt gaattctatt attccaccat ccgcatgacc gccaccggcg
    21301 cggtgaggac tcaatgatgc tcaaacggct tttcccccgc ggcgagaagg ggatcggcgc
    21361 ggtcgagttc gcgctcatcg ccccggtcct gatcggcttc atcgtcggag tcagccaggt
    21421 cggccgcatg ttctacgcca atgcggacat gaagaacgcg agcgcccacg cggcccggct
    21481 cgcttcgatc taccgcaccg gcggaacggt cgacgacgcg acgctcatcg ccgccgtcaa
    21541 ggcccggcta ctgcgcgacg gcgccgcgtc gaaggtcacc gtcaccgtta cccgcggcac
    21601 cgacaacggc aatccctacg tcgagctcaa gaccgactat tcggtgccgc tcgag
//
LOCUS       DASVQZ010000156         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_32469, whole genome shotgun sequence.
ACCESSION   DASVQZ010000156 DASVQZ010000000
VERSION     DASVQZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32469"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VF547_04410"
     CDS             <1..1189
                     /locus_tag="VF547_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX8262097.1"
                     /translation="SLTAHVTIVARAMGIPMLGRIRNVRRVIGEGDYVLLDGNDGIAF
                     VRPTGPVEEAFKAKQVLTQKRRAEFAALRDVPPVTSDGRRIMVMVNAGLRDDIGALDV
                     TGADGIGLFRTEFQFLVSAALPSRESQQRLYKSVIEAAGDKPVIFRTIDVGGDKALPY
                     MTVDEGAHEENPAMGWRALRLALDRDGLMKAQARALLCAAAGRELNVMFPMVSEPWEY
                     DEARALVEQQREWLAGRKKLLPTQIRYGAMLEVPALAETLDLLLPKVDFLSIGTNDLT
                     QFLFAADRAHPKLAERYDWLSPSILRFLRRIVGASDSAGVPVGVCGEMGGRTLEAMAL
                     IGIGLTRFSITPAAVGPIKAMIRSLDRSALLPAMEAMLAKPPADLRGALRAWADAQGV
                     QVG"
     gene            1410..2234
                     /locus_tag="VF547_04415"
     CDS             1410..2234
                     /locus_tag="VF547_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8262098.1"
                     /translation="MADIDQDQLHAQSVGERLRLAREAKGLSLEDVARQTRIPVRHLE
                     HIEREEWDALPAITYSVGFARSYANTIDLDGAQIGAEVRQQLGGARLGNTATAAYYEP
                     ADPARVPPRSIAIAAAVIAVLLVIGYLVWRSSAVDDGSPADVAITGAEEPAASNPAAA
                     RPAPPTLPAPAPANGPVVLTATEDVWLRIDQAGGGAPLYMGTLKAGQSYQVPAGAQSP
                     RLRTARANVLKVAVGGTQVPPLGPPETTISNVSLAPADLIGRAQGGAAPPAAAPSP"
     gene            2324..3211
                     /gene="bamD"
                     /locus_tag="VF547_04420"
     CDS             2324..3211
                     /gene="bamD"
                     /locus_tag="VF547_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX8262099.1"
                     /translation="MLLLLASAAAPAGAQRSESQERRIERLEQELRAVQRRVFPGGAQ
                     VAPEIEPEAAAAGPAGVPASSAVADLTARVDSLEAQLRELTGQVEQNGNRLRQVENAI
                     AQLRDSTGGRLDALERGAAPVAQSPGGGAKGGLPRPVTPSDELGSAASQADDRPAAPA
                     TAATGDAAAEEAYNAGFRLWEAKRYAEAQKALETAAKRYPKSKWASWANNLAGRAYLD
                     AGKPATAAKTFLANYQGNPKGERAADSLYFLGQALVALDKPADACKAYDELQDVYGTT
                     MRAWLRERLPAARSQAKCR"
     gene            complement(3233..3382)
                     /locus_tag="VF547_04425"
     CDS             complement(3233..3382)
                     /locus_tag="VF547_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262100.1"
                     /translation="MSEDAAFMREQARRCRRLARGIATPDVVATLNQMAVEYDSRAEK
                     MEKKG"
     gene            3541..>3777
                     /locus_tag="VF547_04430"
     CDS             3541..>3777
                     /locus_tag="VF547_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262101.1"
                     /translation="MRARTWAWGGAGLIALAAGTAALAQARGGGTGPAVYWMSADTAS
                     GMAAMAAGMGQGRPSAGAAMGSMFGGGRGRGASYV"
BASE COUNT          556 a         1355 c         1333 g          533 t
ORIGIN      
        1 gtcgctcacc gcccatgtca ccatcgtcgc ccgcgccatg ggcattccga tgctgggccg
       61 catccgcaac gtccgccggg tgatcggcga gggcgattac gtgctgctgg acggcaatga
      121 cgggattgcg ttcgtccgcc ccacgggacc tgtcgaggaa gcgttcaagg ccaagcaggt
      181 gctgacccag aagcgccgcg ccgaattcgc ggcgctgcgc gacgtgccgc cggtcaccag
      241 cgacggccgc cgcatcatgg tgatggtcaa cgccgggctg cgcgacgata tcggcgcgct
      301 cgacgtgacc ggcgccgacg ggatcggcct gttccgcacc gaattccagt tcctcgtatc
      361 ggcggcgctg ccgagccgcg aaagccagca gcgtctttac aagtcggtga tcgaggcggc
      421 gggggacaag ccggtcatct tccggacgat cgacgtcggc ggcgacaagg cgcttcctta
      481 catgaccgtc gacgaaggcg cgcacgagga aaatccggcg atgggctggc gcgctctgcg
      541 cctcgccctc gatcgcgacg gcctgatgaa ggcgcaggcc cgcgccctgc tctgcgccgc
      601 cgccggccgg gagctcaacg tcatgttccc gatggtttcc gagccctggg aatatgacga
      661 ggcccgggcc ctcgtggagc agcagcgcga atggctggcc gggcgcaaga aattgctgcc
      721 gactcagata cgctacggcg cgatgctcga ggtgccggcg cttgccgaga cgcttgacct
      781 gcttctgccc aaggtcgatt tcctatcgat cggcaccaac gacctcaccc agttcctgtt
      841 cgccgcggat cgcgcccatc ccaagctcgc cgagcgctac gactggctca gcccttcgat
      901 cctgcgcttc ctcaggcgca tcgtcggcgc gtcggattcg gccggggtgc cggtcggggt
      961 gtgcggcgag atgggcggtc ggacgctgga ggcgatggcg ctgatcggga tcggcctcac
     1021 gcgcttctcg atcacgccgg cggcggtcgg cccgatcaag gcgatgatcc gatcgctcga
     1081 ccgctcggcg ctgctgccgg cgatggaggc gatgctggcg aagccgccgg ccgacctgcg
     1141 cggggcgctc agggcctggg cggacgcgca gggggttcag gtgggttagc caactcctcc
     1201 ccggcatctg ccggggaggg ggaccggccg tcaggccggt ggaggggttc ttcggcggtg
     1261 cagaccctcc accgcgcgac gcgcggtccc cctccccgcg aaatcgcggg gaggaattga
     1321 ggcggcgacc aatcgtgccg cagtccggtt gactttgccc gcgctccccg tctctatgat
     1381 tgatccatat gggaggaaaa ccgggccaga tggcggatat cgatcaggac cagcttcacg
     1441 cccagtcggt cggcgagcgg cttcgcctcg cccgcgaggc gaaggggcta agcctggagg
     1501 acgtcgcccg ccagacgagg atcccggtcc gccatctcga gcatatcgag cgcgaggaat
     1561 gggacgcgct ccccgccatt acctacagcg tcggcttcgc gcgcagctac gccaacacga
     1621 tcgacctcga cggcgcgcag atcggcgccg aggtccgcca gcagctcggc ggcgcccgcc
     1681 tcggcaacac cgcgaccgcc gcttattacg agcccgccga cccggcgcgg gtgccgccgc
     1741 gctcgatcgc gatcgccgcc gcggtcatcg ccgtcctgct cgtcatcggc tatctcgttt
     1801 ggcgcagcag cgcggtcgac gacggctcgc ccgccgacgt tgcgatcacc ggggccgagg
     1861 agccggccgc gtccaacccg gcggcggccc gcccggcgcc cccgacgttg ccggcccccg
     1921 ctccggccaa cggcccggtc gtgctcaccg ccacggagga cgtgtggctg cggatcgacc
     1981 aggcgggcgg cggcgctccc ctctacatgg ggacgctcaa agccggacag agctaccagg
     2041 tgcctgcagg ggcgcagtcg ccgcggcttc ggaccgcccg cgccaacgtg ctcaaggtcg
     2101 ccgtcggcgg gacccaggtc ccgccgctgg gtccgcccga gacgacgatc tccaacgtca
     2161 gcctggcccc cgccgacctc atcgggcgcg cgcagggcgg cgcggcgccg ccggccgcgg
     2221 caccgtcacc gtaataattc actcttctac cccgccgcca agccgtttag gatggccgtc
     2281 gggacgccag gggcggggga ttgtcatgcg ttttcatctt gttgtgttgc tccttctagc
     2341 gagcgcggcg gcgccggccg gcgctcagcg cagcgagagc caggaacgcc gcatcgaacg
     2401 gctcgagcag gagctgcggg cggtgcagcg cagggtcttc ccgggcggcg ctcaggtcgc
     2461 tcccgagatc gagccggagg ccgccgcggc cggcccggcg ggcgtgccgg cgtccagcgc
     2521 cgtcgccgac ctcaccgccc gcgtcgattc gctcgaggcc cagcttcgcg agctgaccgg
     2581 acaggtcgag cagaacggca accggcttcg ccaggtggag aacgccatcg cccagcttcg
     2641 cgacagcacc ggcggccgcc tcgacgcgct cgagcgcggc gccgccccgg tcgcccagtc
     2701 cccgggcggg ggtgcgaaag gcggcctccc gaggccggtc accccttccg acgaactggg
     2761 gtcggcggcg tcgcaggccg acgaccggcc cgccgctccc gccactgccg caaccggcga
     2821 cgccgccgcc gaggaggcct ataacgccgg cttccggttg tgggaggcga agcgttacgc
     2881 cgaagcacag aaggcgctcg agacagcggc caagcgctat cccaagagca agtgggcgag
     2941 ctgggccaac aacctcgccg gccgcgccta tctcgacgcg ggcaagccgg ccacggcggc
     3001 caagaccttc ctcgccaact accagggcaa tccgaagggg gagcgcgccg ccgacagcct
     3061 ctacttcctc ggccaggccc tggtcgcgct cgacaagccg gccgacgcct gcaaggccta
     3121 tgacgagctc caggacgtct acggaacgac gatgcgcgcc tggctgcgcg agcgcctgcc
     3181 cgccgcgcgt tcacaggcga agtgccgctg acggatccgc aaggcccgcg cctcagccct
     3241 ttttctccat cttctccgcg cgcgagtcat attcgaccgc catctggttg agggtggcga
     3301 cgacgtcggg ggtggcgatg ccgcgggcaa gacggcggca gcgacgggcc tgctcccgca
     3361 tgaaggcagc atcctcgctc atgcggagtc agcgaaaacg gtgcggccgc cgttccattc
     3421 ttcgcgataa ccggttcatt gcgacggacc tttcgcaacc gatacggttc gcgctccctt
     3481 ttgcgcccat gcctggacgc cgctataggc ggccggatcg aaccaacagg ggatgacgac
     3541 atgagggcga ggacatgggc ttggggcggc gcgggcctga tcgcgctggc ggccggcacg
     3601 gcggctctgg cgcaggcgcg gggcggcggc acggggccgg ccgtctattg gatgagcgcg
     3661 gacaccgcct cgggcatggc ggccatggcc gcgggcatgg gccagggccg gcccagcgcc
     3721 ggcgcggcga tgggctcgat gttcgggggc ggccgcggcc gcggcgcctc ctatgtc
//
LOCUS       DASVQZ010000157         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_513093, whole genome shotgun sequence.
ACCESSION   DASVQZ010000157 DASVQZ010000000
VERSION     DASVQZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513093"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VF547_04435"
     CDS             <1..387
                     /locus_tag="VF547_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX8262102.1"
                     /translation="DVARSRDDRALLLAAVVAPGGICPALCEALEAGGPYGAGWPAPR
                     VAAGPVRVVKADVVGSGHLGLIVAGDDGRRVKAIAFRMAQGPLGEAMLAAPPYRKLWI
                     AGRVRADEWEGRVGAELHLEDAAWAD"
     gene            430..505
                     /locus_tag="VF547_04440"
     tRNA            430..505
                     /locus_tag="VF547_04440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:464..466,aa:Glu,seq:ttc)
     gene            535..1617
                     /locus_tag="VF547_04445"
     CDS             535..1617
                     /locus_tag="VF547_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HEX8262103.1"
                     /translation="MLAYFKGSFLFTVVCLALAVWLGWAMTGSVGGTAGILWIVVVLS
                     VLEVSLSFDNAVVNAAVLREMSPIWRKRFLTWGIAFAVFGMRIVFPLAIVAIAAQLGP
                     IEAIRLAAAEPAEYERIITSAHVGIAGFGGAFLAMVGLKFFFDSEKEVHWIDVIETRL
                     SRLSSVDAIEIGLVLLTLYGISNLLPREEALTYLVAGIFGILAYLAVEAVNSLLEPEE
                     TGVAGAAVRSGLAGFLYLNLLDSAFSFDGVIGAFALSNNIFVIALGLGIGAMFVRSMT
                     ILLVDKGTLAQYRYLEHGAFWAILALAAIMLLSARYHIPEVVTGLIGALLIGLSLYAS
                     ARHNRRQPEAEAKRGPVGADGRRLDL"
     gene            complement(1619..2710)
                     /locus_tag="VF547_04450"
     CDS             complement(1619..2710)
                     /locus_tag="VF547_04450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8262104.1"
                     /translation="MGGGAEIHAARLATALRARGHSVRIQTVAEDSARTTTNPVLSQG
                     STIARRLYSLRVARTILFGRGRYSVVHFFLGGRHTAIGGLACRVAGCPHVVMYGGSGA
                     FDEMGASLLGRLALKADARLARRLIALNEGMRQSFRACGAPDDRIVILPCEADPDFFS
                     PGDPDAVGAIRTRFAIPPEAHVISFVGRLVTDKALPTLVAAFGRLSRADPRAVLVLAG
                     EGPERPRLEALIRALPEPGRALLTGQLTQIEVRDLLRATDVFALVSAIEGIPSALVEA
                     MAVGLPCVVSDIDGMRDIIAPGVDGLQVPQGDEAALADALLALLSDPPLRLRMGRAAR
                     RHALERYTPAVVAEAHEKLYREILAEAAR"
BASE COUNT          404 a         1001 c         1048 g          473 t
ORIGIN      
        1 gacgtcgcca ggagccgcga cgaccgcgcc ttgctgctcg ccgcggtggt ggcgccgggg
       61 ggcatctgcc cggctttgtg cgaagcgctc gaggcgggcg gcccctacgg ggcgggctgg
      121 ccggcgccgc gggtcgcggc cggtccggtc cgggtggtca aggcggacgt ggtgggaagc
      181 ggccatctgg ggctgatcgt cgccggcgac gacggccggc gggtcaaggc gatcgccttc
      241 cggatggcgc aaggcccgct cggcgaagcg atgctggcgg cgccgccgta ccgcaaattg
      301 tggatcgcgg gccgggtccg cgccgacgaa tgggagggcc gcgtcggtgc cgagctccac
      361 ctcgaggacg ccgcctgggc cgattgacgc agcgcccgcc gccacctaag ggccgcgctt
      421 cggcccatgg gccccatcgt ctagcggtct aggacgtcgc cctttcacgg cgaaaacacg
      481 ggttcgagtc ccgttggggt caccaagcct ggaaccggtc gacgggggcc tgacgtgctc
      541 gcttatttca agggctcctt cctgttcacc gtggtctgcc tcgccctggc ggtgtggctc
      601 ggctgggcga tgaccggctc cgtcggcggg accgccggga tcctgtggat cgtcgtcgtc
      661 ctcagcgtgc tcgaagtgtc gctgtcgttc gacaatgcgg tggtaaacgc ggcggtgctt
      721 cgcgagatgt cgccgatctg gcgcaagcgc ttcctgacct ggggcatcgc cttcgccgtg
      781 ttcgggatgc ggatcgtctt ccccctcgcc atcgtcgcca tcgccgccca attggggccg
      841 atcgaggcga tccggctcgc ggcggcggag ccggccgaat atgagcggat catcacctcg
      901 gcccacgtcg gcatcgccgg cttcggcggc gccttcctcg ccatggtggg cctcaagttc
      961 ttcttcgaca gcgagaagga ggtgcactgg atcgacgtca tcgaaacccg cctgtcgcgc
     1021 ctctcctcgg tcgacgcgat cgagatcggc ctggtcctgc tgaccttgta cggaatctcc
     1081 aacctgctgc cgcgggaaga ggcgctcacc tatctcgtcg cgggcatctt cggcatcctg
     1141 gcatatctcg ccgtcgaggc cgtcaattcg ctgctcgagc cggaggagac cggagtcgcc
     1201 ggagcggcgg tgcggtccgg cctggccggg ttcctctacc tcaacctgct cgattccgct
     1261 ttctcgttcg acggcgtcat cggagccttc gccctttcga acaacatctt cgtgatcgcg
     1321 ctcggcctcg gcatcggcgc gatgttcgtg cgctcgatga ccatcctgct ggtcgacaag
     1381 ggcacgctgg cccaatatcg ctatctcgag cacggcgcct tctgggcgat cctcgccctg
     1441 gccgcgatca tgctgctgtc ggcgcgctac cacatccccg aggtcgtcac cgggctgatc
     1501 ggggcgctgc tgatcggctt gtcgctctac gcctcggcga ggcacaaccg gcgccagccc
     1561 gaggccgagg ccaagcgcgg gccggtcggc gccgacggcc gccgcctgga tctctagcct
     1621 agcgcgcggc ttcggcgaga atctcgcggt agagcttctc gtgcgcctcg gcgacgacgg
     1681 ccggcgtgta gcgctcgagc gcatggcggc gcgcggcgcg ccccatccgc agccgcagcg
     1741 gcgggtcgga cagcaaggcc agcaaggcgt cggccagcgc cgcttcgtcg ccctgcggca
     1801 cttgaaggcc gtccacgccg ggcgcgatga tgtcccgcat gccgtcgatg tcgctgacca
     1861 cgcaggggag gccgaccgcc atggcttcga ccagcgccga gggaattccc tcgatcgcgg
     1921 agaccagggc gaacacgtcg gtggcgcgaa gcaggtcgcg gacctcgatc tgagtcagct
     1981 ggccggtgag gagggctcgt cccggctcgg gcagcgcccg gatgagagct tccagccggg
     2041 gccgctcggg cccttcgccg gcgaggacca ggacggcgcg cggatcggcc cgagacaggc
     2101 ggccgaacgc cgcgaccagg gtggggagcg ccttatcggt gaccagcctg cccacgaagc
     2161 tgatcacgtg cgcttcgggc gggattgcga agcgtgtccg gatcgcgccg acagcgtccg
     2221 gatcgcccgg cgaaaagaaa tcgggatcgg cctcgcaggg gaggatgacg atgcgatcgt
     2281 cgggggcgcc gcacgccctg aagctctggc gcatgccttc gttgagggcg atcaggcggc
     2341 gggcgagccg ggcgtcggcc ttgagggcca gccttcccag caggctggcc cccatctcgt
     2401 cgaaggcgcc gctgccgccg tacatgacga catgggggca gccggccacc cggcaggcga
     2461 ggccgccgat ggccgtatgc cttcctccca ggaagaaatg cacgaccgag tagcggccgc
     2521 ggccgaacag gatcgtccgg gcgacgcgga ggctgtagag gcgccgcgcg atcgtcgagc
     2581 cctggctgag gacggggttg gtcgtggtcc gagcgctgtc ctcggccacc gtttgaatgc
     2641 ggaccgaatg ccctcgcgcc cgcagcgccg tcgcgagcct ggcggcgtgg atctccgccc
     2701 cgcctcccat gggcggcagc gtctggatcg cgaacagaat ggagaggggc gcaggctggt
     2761 cccggctcac aaggattgca cgagacgggc gagcgcctcg gccgcgccca tcttcaccag
     2821 gctgtcgccc gtctggggcg cttccaccgg ataggggatt tcatagcctt ccgggctcag
     2881 atagcgttcc gggtcgacat agacgatctt gaggccctct tcccgc
//
LOCUS       DASVQZ010000158         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_803124, whole genome shotgun sequence.
ACCESSION   DASVQZ010000158 DASVQZ010000000
VERSION     DASVQZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803124"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VF547_04455"
     CDS             <1..926
                     /locus_tag="VF547_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8262105.1"
                     /translation="KKVAELYLTPEQMAEIKSLGSWEEIMETLKQRLAEQQGRHQGGN
                     KWIGTGGTSPFGNSGYNPEGIRIGGEGGQKSAIKVWDKREFRNLDSSKELGTRNIKVA
                     LRRLRRFAREGAAEELDIDETIDGTARRGWLDIRMRPERHNAVKLLLLLDIGGSMDPH
                     VQVCEELFSAAHGEFKNLEFFYFHNCPYESLWKDNRRRFAERTPTWDVLHKFGHDWKI
                     VFVGDASMSPYEVTHPGGSVEHYNEEAGAVWMQRLVDTYPSAVWLNPVPEKYWGYSQS
                     TQIVRQLMRERMYPLTLAGLDDAMRALSRKT"
     gene            complement(979..1440)
                     /gene="soxR"
                     /locus_tag="VF547_04460"
     CDS             complement(979..1440)
                     /gene="soxR"
                     /locus_tag="VF547_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEX8262106.1"
                     /translation="MPAPALLTIGELARRTGLSVSAIRFYEARGLVGAIRTSGNQRRF
                     LRSDVRRLSFALIAQRCGLTLAEIEAELGRLPLGRAPTRADWAAISTRLRARLDERIA
                     MLEATRDRLDGCIGCGCLSLDVCALYNPEDRAGGAGPGPRYLLGDKAADFA"
     gene            1531..1911
                     /locus_tag="VF547_04465"
     CDS             1531..1911
                     /locus_tag="VF547_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8262107.1"
                     /translation="MALPRIEHVNVTVSDPERAARLMEKLFGWQVRWQGAAQKGGHTI
                     HVGSADHYVAVYTDPARTYSSDQFVKGRPLNHIGVEVDDLDATEARVVEAGLTPFSHG
                     DYEPGRRFYFLDPDGIEYEVVSYR"
     gene            complement(2025..2621)
                     /gene="recR"
                     /locus_tag="VF547_04470"
     CDS             complement(2025..2621)
                     /gene="recR"
                     /locus_tag="VF547_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX8262108.1"
                     /translation="MASAEIDALTQALARLPGLGPRSARRAVLHLMKKREAALVPLLK
                     ALERVSENLSVCSACGNVDTTDPCSVCADPRRDDRLLCVVEEVSDLWALDRSRLFPGR
                     FHVLGGRLSALEGVRPEDLSIDKLLGRVARGGIDEVVLAMNATLEGQTTAHYLAERLE
                     TYPIRLTQLAHGLPVGGELDYLDEGTLAQALRARRPVA"
     gene            2735..>3475
                     /gene="fmt"
                     /locus_tag="VF547_04475"
     CDS             2735..>3475
                     /gene="fmt"
                     /locus_tag="VF547_04475"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX8262109.1"
                     /translation="MRLAFMGTPDFAVPTLEALIAAGHEVAAVYCQPPRPAGRGKALR
                     PSPVQRRAEAAGIGVRTPLTLRDPDAQADFAALELDAAVVAAYGLILPRAILDAPRLG
                     CLNVHASLLPRWRGAAPVQRAILAGDRMTGVTIMQMEEGLDTGPMLAAMGTPIDRKTA
                     GALTDELARLGAEWMVETLARIHRIGPRPQEEAKATYAPKIRKEEARLDFTRPALALE
                     RQVRAFNPAPGAWFEVAGERIRLLAAEPV"
BASE COUNT          579 a         1219 c         1206 g          471 t
ORIGIN      
        1 tgaagaaggt cgcggagctc tatctgacgc cggagcagat ggccgagata aagtcgctcg
       61 gctcatggga agaaatcatg gagacgctga agcagcggct cgccgagcag caggggcgcc
      121 accagggcgg caacaagtgg atcggcaccg gcggcacctc gccgttcggg aactccgggt
      181 acaatcccga aggcatccgg atcggcggcg aaggcgggca gaagagcgcg atcaaggtct
      241 gggacaagcg cgagttcagg aacctcgaca gctccaagga actcggcacc cgcaacatca
      301 aggtcgcgct caggcggctg cggcgcttcg cccgcgaagg cgcagccgaa gagctcgaca
      361 tcgacgagac gatcgacggc accgcgcgcc gcggctggct cgacatccgg atgcggcccg
      421 agcgccacaa tgcggtgaag ctgctgctcc tgctcgacat cggcgggtcg atggacccgc
      481 acgtccaggt ctgcgaggag ctgttctcgg ccgcgcacgg cgagttcaag aatctcgagt
      541 tcttctactt ccacaattgc ccctacgaga gcctgtggaa ggacaaccgc cgccgcttcg
      601 ccgagcgcac gccgacctgg gacgtgctcc acaagttcgg ccacgactgg aagatcgtct
      661 tcgtcggcga cgcctcgatg agcccctacg aggtcaccca tccgggcggc tcggtcgagc
      721 attacaacga ggaggccgga gcggtctgga tgcagcgcct cgtcgacacc tatccgagcg
      781 cggtctggct caatccggtg cccgagaaat attggggcta cagccagtcc acccagatcg
      841 tccgccagct gatgcgcgag cgcatgtacc cgctcaccct cgccggcctc gacgacgcga
      901 tgcgcgcgct gagccggaag acatgaaatg gggacacgta cttttcgtgc cgaaaagtac
      961 gtgtccccct cgccggcatc aggcaaagtc cgccgctttg tcgccgagca gataacgcgg
     1021 ccccggcccc gctccgccag cgcggtcctc cggattgtag agggcgcaga cgtcgagcga
     1081 caggcagccg cagccgatgc agccgtcgag ccggtcgcgg gtcgcctcga gcatcgcgat
     1141 ccgctcgtcg agcctggctc ggagccgggt gctgattgcc gcccagtcgg cccgcgtcgg
     1201 cgcccggccg agcggcagcc gccccagctc ggcctcgatc tcggccaggg tcaggccgca
     1261 gcgctgggcg atcagggcga aggacaggcg gcggacgtcg gagcgaagga agcggcgctg
     1321 gttgcccgag gtgcggatcg ccccgaccag cccgcgcgcc tcgtagaagc ggatcgccga
     1381 gaccgagagg ccggtgcgcc gggcgagctc gccgatggtg aggagggccg gcgcgggcat
     1441 cggggcgtcc ttcgaaaatg aagcttgacc tcaacttagc ttgaggtcgt acgactcgca
     1501 atcgagggcg cattggcagg aggaaattcg atggcgttac ccaggatcga gcatgtcaac
     1561 gtgacggtca gcgaccccga gcgggcggct cggctgatgg agaagctgtt cggctggcag
     1621 gtgcgctggc agggcgcggc gcagaagggc gggcatacga tccacgtcgg atcggccgac
     1681 cattatgtcg ccgtctacac cgatccggcc aggacctatt ccagcgacca gttcgtcaag
     1741 ggccggccgc tcaaccatat cggagtcgag gtcgacgatc tcgacgccac cgaggcgcgg
     1801 gtcgtcgagg ccggcctcac ccccttctcg cacggcgatt acgagccggg ccggcgcttc
     1861 tacttcctcg acccggacgg gatcgaatat gaggtggtga gctaccggta gcgtaccccc
     1921 tccctgcacg cagggagggg ttgggggtgg gtacgtcaga cgcggcaatg ccgatccgga
     1981 gacgcacccg ccccccagcc ccacccggcg tgcagcgagg ggcgctaggc caccggccgc
     2041 cgcgcccgaa gcgcctgggc gagcgtgcct tcgtccagat agtcgagctc gccgccgacc
     2101 gggaggccgt gggcgagctg ggtgagtcgg atcgggtagg tctccagccg ctcggccaaa
     2161 taatgggcgg tggtctggcc ctccagcgtc gcgttcatgg cgaggacgac ctcgtcgatc
     2221 ccgccccggg cgacgcggcc gagcagcttg tcgatgctga gatcctccgg ccggacacct
     2281 tccagggccg agagccggcc gccgaggacg tggaagcggc cggggaagag tcgcgagcgg
     2341 tcgagcgccc acaggtccga cacctcctcg acgacgcaga gcagccgatc gtcgcggcgc
     2401 ggatcggcgc agacgctgca cggatcggtc gtgtcgacat tgccgcaggc cgagcagacc
     2461 gagaggttct cgctgacccg ctccagcgcc ttcagcagcg gcaccagcgc cgcctcgcgc
     2521 ttcttcatca ggtgaagcac cgcccgccgc gccgagcgcg gcccgagccc gggcaggcgg
     2581 gcgagcgcct gggtgagcgc atcgatctcg gcggaagcca tggggggtag gtagcgttcc
     2641 tcacccgtca ttgcgagcgt agcgaagcaa tccagcgcgg cctccggggc agaactggat
     2701 tgcttcgtcg cttcgctcct cgcaatgacg aaacatgcgc ctcgccttca tgggaacccc
     2761 cgatttcgcc gttccgacgc tggaggccct gatcgccgcc ggccacgagg tcgccgccgt
     2821 ctattgccag ccgccccgcc ccgccggccg cggcaaggcg ctccgcccct cccccgtcca
     2881 gcgcagggcc gaggcggcgg gaatcggggt ccgaaccccc ctcaccctcc gcgatccgga
     2941 cgcccaggcc gacttcgccg cgctcgagct cgacgccgcc gtcgtcgccg cctacggcct
     3001 gatcctgccg agggcgatcc tcgacgcgcc gcggctcggc tgcctcaacg tccacgcctc
     3061 gctgctgccg cgctggcgcg gcgccgcccc cgtccagcgc gccatcctcg ccggcgaccg
     3121 gatgaccggg gtgaccatca tgcagatgga ggagggcctc gacaccggcc cgatgctggc
     3181 ggcgatgggc acgccgatcg accgcaagac cgccggcgcg ctgaccgacg agctcgcccg
     3241 cctcggcgcc gaatggatgg tcgagacgct cgcccggatc caccggatcg gcccgcgtcc
     3301 gcaggaggag gccaaggcga cctacgcgcc caagatccgc aaggaggagg cccggctcga
     3361 cttcacccgg ccggcgctcg cgctggagcg gcaggtacgg gccttcaacc ccgcgcccgg
     3421 cgcctggttc gaggtggccg gcgagcggat caggctgctc gcggccgagc cggtc
//
LOCUS       DASVQZ010000159         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_707701, whole genome shotgun sequence.
ACCESSION   DASVQZ010000159 DASVQZ010000000
VERSION     DASVQZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707701"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF547_04480"
     CDS             complement(<1..720)
                     /locus_tag="VF547_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEX8262110.1"
                     /translation="MPSGLAALLDDVAAIAKLAAASVDDVGAAAGRAGAKAAGVVIDD
                     TAVTPRYVTGFTPDRELPIIARIAKGSLKNKLLILLPAALALSAFLPWAITPLLMIGA
                     AYLCFEGVEKLWEAIAEREHSLAEEVAELSSADHERQMVAGAVRTDFILSAEIMAIAL
                     AEVAAETFLSRAVILAVVAVAITVGVYGLVGLIVKMDDIGLHLAERRNRGSQAVGRAL
                     VHAMPKVMAALSTIGIAAMIWV"
     gene            857..1648
                     /locus_tag="VF547_04485"
     CDS             857..1648
                     /locus_tag="VF547_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX8262111.1"
                     /translation="MDAITTLLSDPAAWAALVTLLVMEIVLGIDNLIFISILSNKLPE
                     AQRQRVRRLGIGLALVMRLVLLSTIAWLVGLKAPVFDLGLAGPADAHGTPAFETAFSW
                     RDLILIAGGLFLLWKATKEIHHNVQPGDEDDVFDTTSRATLGAGAAIVQILLLDIVFS
                     VDSILTAVGMTDHLPIMVVAVVVAVGVMLFAADPLANFINRNPTVVMLALGFLLMIGA
                     VLIADGFGIHVPKGYIYAAMAFSAGVESLNIIARRRSQARRGRSE"
     gene            1721..2338
                     /locus_tag="VF547_04490"
     CDS             1721..2338
                     /locus_tag="VF547_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8262112.1"
                     /translation="MTVHFHEEDLPEGVLAPGPVAVDTEAMGLLPGRDRLCLVQIADG
                     RGDEHLVRFAPGSDYAAPNLKAVLADPGRLKLYHFARFDLAIMQAYLGVMAGPVYCTK
                     TASRLVRTYTDRHGLKELIKELLQIDISKQQQTSDWGAPDLTDAQKDYAASDVRYLHQ
                     MKEKLDERLEREGRMKLAQACFDFLPHRALLDLAGWAEVDIFAHV"
     gene            2331..2969
                     /gene="lptC"
                     /locus_tag="VF547_04495"
     CDS             2331..2969
                     /gene="lptC"
                     /locus_tag="VF547_04495"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX8262113.1"
                     /translation="MSELARGRRAAKRGWAAPGGLHDWLMRFLKVILPAGVGLLLAYL
                     LLSPLSKEKEISFLLDKNKVDQARERMKMQAAEYRGLDDEGRPFTIQAAQAVQAKSSV
                     PIVDIGGMAARIELKEGPARITADRARYNIETQKVDVLGPILFTAADGYRLETRDVAL
                     DLNRHTAVSGSGVEGRMRLGRFTAGGMSVDLPQRRVVLTGRPRLHIEQGGLR"
     gene            2975..3493
                     /locus_tag="VF547_04500"
     CDS             2975..3493
                     /locus_tag="VF547_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX8262114.1"
                     /translation="MKRLAPLLLLAALGLSPATGQVSALKGHDSDAPVDVAADRIEVQ
                     DRADRAIFSGNVVVRQGGLTLTAARLTVAYSGGGGVQLRRIDATGGVTVRGPAETARG
                     DVGIYDLERRIITLIGGVSLVQKDAKVNGGRLTIDLDSGRAVIDGGGPPGVTSQGGRV
                     TGTFTVPPRKGG"
     gene            3577..>4049
                     /locus_tag="VF547_04505"
     CDS             3577..>4049
                     /locus_tag="VF547_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8262115.1"
                     /translation="MNDAAALPRANEAEVFAELARGLCVVSIAKSYDRKTVLKDVSLH
                     VERGEVVGLLGPNGAGKTTCFYSIMGLVKPDRGSILLDGQDITGLPMYRRAVLGLGYL
                     PQETSIFRGLTVAQNIMAVLEVAEPDRTVRRERLEQLLAEFHIGHLRDAPAMALSG"
BASE COUNT          609 a         1469 c         1345 g          626 t
ORIGIN      
        1 gacccagatc atcgcggcga tgccgatcgt ggagagcgcc gccatcacct tgggcatggc
       61 gtggaccagc gcccggccca ccgcctgcga tccgcggttg cggcgctcgg cgagatgcag
      121 gccgatatcg tccatcttca cgatgagccc gaccagcccg tagactccga ccgtgatcgc
      181 caccgcgacc acggcgagga tgacggctcg gctcaggaag gtctcggccg cgacttcggc
      241 gagcgcgatc gccatgatct cagccgagag gatgaagtcg gtgcgcaccg cgcccgccac
      301 catctggcgc tcgtggtcgg cgctgctcag ctcggcgacc tcctcggcga ggctgtgctc
      361 ccgctcggcg atggcctccc acagtttttc caccccctcg aagcacagat aggccgctcc
      421 gatcatcagc aggggcgtga tcgcccaggg gaggaacgcg ctgagggcga gcgccgccgg
      481 cagcaggatg aggagcttgt tcttcagcga ccccttggcg atgcgggcga tgatcggaag
      541 ctcgcggtcg ggcgtgaagc cggtgacgta gcgcggagtc accgccgtgt cgtcgatgac
      601 gacgcccgcc gccttggccc cggctcggcc ggcggcggcg ccgacgtcgt cgaccgacgc
      661 ggcggcgagc ttggcgatgg cggcgacgtc gtcgagcaag gcggcgagac cggaaggcat
      721 ggatgctcct gtggcggggc ggcgacacgc gatcaaccct ttaccctcac gtcctccccg
      781 agagtcctcg gggaggactt gaatcggcgc cgttcaccgc gttgaccttc cgcgcctatc
      841 ccctatggcg ccggccatgg acgccatcac caccctcctc tccgaccccg cggcctgggc
      901 ggcgctcgtc accctgctcg tgatggagat cgtgctcggg atcgacaatc tcatcttcat
      961 ctcgatcctg tcgaacaagc tgcccgaggc gcagcgccag cgcgtccgcc ggctcggcat
     1021 cgggctcgcc ctggtcatgc gcctcgtcct gctctcgacc atcgcctggc tggtcgggct
     1081 gaaggcgccg gtcttcgacc tcggcctcgc cggccccgcc gacgcccacg gcacgcccgc
     1141 cttcgagacc gccttctcct ggcgcgatct catcctgatc gcgggcggcc tcttcctgct
     1201 ctggaaggcg accaaggaga tccaccacaa cgtccagccc ggcgacgagg acgacgtctt
     1261 cgacaccacc tcgcgggcga cgctcggggc gggcgccgcg atcgtccaga tcctcctgct
     1321 cgacatcgtc ttctcggtcg attcgatcct caccgccgtg ggcatgaccg accatctccc
     1381 gatcatggtc gtcgccgtcg tcgtcgcggt gggggtgatg ctgttcgcgg ccgatccgct
     1441 cgccaacttc atcaaccgca atccgacggt ggtgatgctg gcgctcggct ttctgctgat
     1501 gatcggcgcg gtcctgatcg ccgacggctt cggtatccac gttcccaaag gctatatcta
     1561 cgccgccatg gccttctcgg cgggggtcga atcgctcaac atcatcgccc gccgccgaag
     1621 ccaggcgagg cgggggcggt cggagtagcg aaaggccctc tgcccgcggg gagaggggta
     1681 ggtgattgag cgtcaaggcg cttcggccta tgcgaggccc atgaccgtcc atttccatga
     1741 ggaggacctg cccgaaggcg tcctcgcccc cggtccggtg gccgtcgata ccgaggcgat
     1801 gggcctgctg ccgggccgcg accggctctg cctggtccag atcgccgacg gccgcggcga
     1861 cgagcatctc gtccgcttcg ccccggggag cgactatgcc gcgcccaacc tcaaggcggt
     1921 cctcgccgat ccgggccggc tcaagctcta ccatttcgcc cgcttcgacc tcgccatcat
     1981 gcaggcctat ctcggagtga tggcgggacc ggtctattgc accaagaccg cctcgcgcct
     2041 cgtccgcacc tataccgacc ggcacggcct caaggagctg atcaaggagc tgctccagat
     2101 cgacatctcc aagcagcagc agacctcgga ctggggcgct cccgacctca ccgacgcgca
     2161 gaaggactat gccgcctcgg acgtgcgcta tctccaccag atgaaggaga agctcgacga
     2221 gcggctcgag cgggaaggcc ggatgaagct ggcgcaggcc tgcttcgact tccttccgca
     2281 ccgggccctg ctcgacctcg ccggctgggc cgaggtcgac atcttcgccc atgtctgagc
     2341 tggcccgcgg ccggcgggcg gcgaagcgcg gctgggcggc gccgggcggc ctgcacgact
     2401 ggctgatgcg cttcctgaag gtgatcctgc ccgccggagt cgggctgctg ctcgcctatc
     2461 tcctgctctc gccgctgtcg aaggagaagg agatcagctt cctgctcgac aagaacaagg
     2521 tcgatcaggc gcgcgagcgg atgaagatgc aggcggccga atatcgcggg ctcgacgacg
     2581 aggggcgccc gttcaccatc caggccgcgc aggcggtgca ggccaagtcg agcgtgccga
     2641 tcgtggacat cggcggcatg gccgcgcgca tcgagctcaa ggaagggccg gccaggatca
     2701 ccgccgatcg cgcccgctac aacatcgaga cccagaaggt cgacgtgctc ggcccgatcc
     2761 tgttcacggc cgccgacggc taccggctcg agacccgcga cgtcgcgctc gatctcaaca
     2821 ggcacaccgc cgtcagcggc agcggggtcg agggacggat gcgcctcggc cggttcaccg
     2881 ccggcgggat gagcgtcgac ctgccgcagc ggcgcgtggt cctcaccggc cgtccgcgct
     2941 tgcatatcga gcagggcggg ctcagatgag cggcatgaag cgcctcgctc cccttctgct
     3001 cctcgccgcg ctcggcctca gccccgcgac cgggcaggtg tcggccctca agggccacga
     3061 cagcgacgcg cccgtcgacg tcgcggccga ccggatcgag gtgcaggacc gcgccgaccg
     3121 ggccatcttc tccggcaacg tcgtcgtccg ccagggcggc ctgaccctca ccgccgcgcg
     3181 cctcaccgtc gcttattcgg gcggcggcgg agtccagctg cgccgcatcg acgccaccgg
     3241 cggagtgacc gtccgcggcc cggccgagac ggcccgcggc gacgtcggca tctacgatct
     3301 cgagcggcgc atcatcaccc tgatcggcgg agtcagcctg gtccagaagg atgcgaaggt
     3361 gaacggcggg aggctgacca tcgacctcga cagcggccgg gcggtgatcg acggcggcgg
     3421 cccgcccggc gtcaccagcc agggcggccg ggtcaccggc accttcaccg tgccgccgcg
     3481 caagggcggc taggccggtc gccgggccgc ttttttcgtc catgcatgaa ccctgccagt
     3541 tgagccgtta accggcatcg cctagtctcg accggcatga acgacgccgc cgcccttccc
     3601 cgcgccaacg aggccgaggt cttcgccgag ctcgcgcggg gcctgtgcgt cgtctcgatc
     3661 gccaaatcct acgaccgcaa gacggtgctc aaggacgtct cgctccacgt cgagcgcggc
     3721 gaggtggtcg gcctcctcgg ccccaacggc gccggcaaga ccacctgctt ctattcgatc
     3781 atggggctgg tgaagcccga ccgcggctcc atcctgctcg acgggcagga catcaccggc
     3841 ctgcccatgt accggcgcgc ggtgctcggc ctcggctatc tgccgcagga gacctcgatc
     3901 ttccgcggcc tcaccgtcgc ccagaacatc atggccgtgc tcgaggtggc ggagcccgac
     3961 aggacggtgc ggcgcgagcg gctcgagcaa ctgctcgcgg agttccacat cggccatctt
     4021 cgcgacgctc cggcgatggc gctgtcggg
//
LOCUS       DASVQZ010000160        10558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_803344, whole genome shotgun sequence.
ACCESSION   DASVQZ010000160 DASVQZ010000000
VERSION     DASVQZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803344"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VF547_04510"
     CDS             <1..1152
                     /locus_tag="VF547_04510"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEX8262116.1"
                     /translation="DRLDPDAAEGIVEAIRASGRLRLGGRREIGFDEPRDLRFLQRER
                     LPHHSNGMRFTAFDAAGEALESAVSYSVGGGAVVDEAAIARNAPPDGFWDIPYNYHCA
                     DELLAISDREGLTIADIARANERAALPDAEIDRRLDAISAAMSQCIDRGIGRDGILPG
                     GLKVKRRAPALHKLLLERAERTLSDPLTVLDWVNLWALAVNEENAAGGRVVTAPTNGA
                     AGIVPAILRYYQRFVPHSDQRGVRDFLLTAAAIGSLFKENASISGAEVGCQGEVGVAC
                     SMAAAGLTAALGGTPWQIENAAEIGMEHNLGLTCDPVGGLVQVPCIERNAVGAIKAIE
                     ASRLALVGTGDHLVSLDQVIETMRKTGLDMNERYKETSLGGLAVNVVEC"
     gene            1303..3231
                     /locus_tag="VF547_04515"
     CDS             1303..3231
                     /locus_tag="VF547_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8262117.1"
                     /translation="MRLRHVLLAAAAFLAAPALAEVEKPAALVADGIPAVPDELAEKT
                     RPYMEFRSAGLQGWNPKTRAIAITTRFGNTAQVHEVARPLGMRRQVTFEADAIAGASY
                     ARRIGDVLVVQKDVGGSEFWQLYVLEGGRLKLITDGKSRNSINNWSHDGRWLAYTSTR
                     RNGADNDIWIVDPRNPASNRLLAEVKGGGWGLADFAPDGRSAVVAEYLSVNKTNLYRV
                     DIATGRMTPIGDHSKQISYGASQFAPNGTLWVTSDEGSDFQRLGILNPTTGRFTAVAP
                     EPRWDVENFDISDDGRFIAYTVNEAGISRPKLLDTRTRKIRSVAGLPVGTMGGLEVAP
                     WGDVAVSVSSARSPSDVYVVNPRTLAVTRWTESETGGLDPRRNVEPVLVETTSFDGEK
                     VSGFLYRPDPARFPGRRPVLVNIHGGPEGQSRPGFLGRTNYYLNELGIAIFYPNVRGS
                     TGFGKRFVALDNGPDLRENSVRDIGAFLDRLAADPGLDPARIGVTGGSYGGYMCYASA
                     IHYGERLKGANCAVAISNFVTFLENTQSYRRDLRRVEYGDERDPAQRKKLLAISPLTR
                     VKELRLPLMVQTGGNDPRVPASEAEQVIKAVRRNDGTAWHLLAQDEGHGFAKKVNQDY
                     AFWTSLMFWQRNLLGERR"
     gene            complement(3358..4473)
                     /locus_tag="VF547_04520"
     CDS             complement(3358..4473)
                     /locus_tag="VF547_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8262118.1"
                     /translation="MRIGAWAARIASGVAVLAALAAFADVATYDAAAWNADFDRLKAD
                     MAQGYANLDWIAERRGLDLAALSIETKSEIENAGSHLIALRALNRFIDRFDDPHFQLA
                     DRDRRAAEGAPQADPPVPDCAGAGYREGRHDFRFPVERLPGWRPLAAGNFPSGLTGST
                     GMLRIAEFGENQYLAACAARFRPGMSERALQLAVRASLQAELRDRLGELRRAGAKRLI
                     VDLSRNGGGSEWVDEAVPLFTARRLERKAPRRVGPACDRRSVWRGKRPPCSVFAKPDP
                     PVRMAGEGAWSGPLLVLIDGDTASASEDFVAWMKDNRAATLIGARTFGAGCGYVDGGT
                     RTRLRAIPRDVRMPNCARFLKDGTNEIEGVAPTSDCR"
     gene            complement(4470..5666)
                     /locus_tag="VF547_04525"
     CDS             complement(4470..5666)
                     /locus_tag="VF547_04525"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8262119.1"
                     /translation="MRRLALLMPLALGACASVADPSPSPPPAGTYAWAAFDTRGVTAS
                     GAQGLADRGSGRALTIDDPVRIASITKLHVALGVMRLVEQGKIDLDEDVSRHLGWTLR
                     NPSFPDRPITLRLLLSHRSSLRDDISYALPLGMTVEQALAAPEAWDKEHRPGTYFRYS
                     NLGFPVVASVLERAAGERFDRLMERLVLKPLALDACFNWTTCTDARIARAAVLYAPDG
                     AVVRDDLKGKRPDCPVVPDSNGGCDLSDYRLGSNGALFSPQGGLRISVRDLARVGGML
                     LRRGRLPDGGRFLSESSLAEIQRIHWRFDGSNGDTEGGFYCGYGLAVQILAQCAPKDD
                     PFGDGKPRLGHAGDAYGLRSGLWIDANGGRGIAYFATALGDDPPRGRSAYRAIEEWLA
                     AKGPRK"
     gene            5756..7537
                     /locus_tag="VF547_04530"
     CDS             5756..7537
                     /locus_tag="VF547_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262120.1"
                     /translation="MKTPALLLMLSLALSAAAAQPRPAPREWSPAQARAVLDKTQTIR
                     LAPSLAHLSPGERTAVAKLLEVGRIFQDVYEAQRHRDALGARAALSRRRDPHGRSLDA
                     LYRLNQGPIATTLDNRREPFLAVPEAPPGKNVYPWDLGKAELDAFVAAHPAERARLAD
                     LRSVVRRGELAALRRDLAKLRQYPAIDMLHPGLRARLEALARRPSRAVLYAVPYSLAY
                     ADEMVRSHRLLNEAADAVEPSDWQFARYLRNRARDLLSDDYESGDAAWITGRFRNLNA
                     QIGAYETYDDELLGTRAFYGLSLLATRNAESAALKDAMKGMQELENSLPTERHKKVIE
                     DIPVGVYDVIADFGQTRGGNTATNLPNEAYLAARYGSTILLRANIMRNPDIFQGSGDI
                     WAAAMAPAFAGHLTADSNFYRTLWHEVGHYLGPDTTVSGASLDSIGADSNLLEELKAD
                     LVSLFVARELRKRGYYSDEQLRSLYASGILRTLQNNKPRREQPYNMMQLMQWNWFLDR
                     RVLSFDPASSKLSIDYDRYHEAVRDLLKQVIAIQESGDAKAAAAFIDRWGTWDDNLHG
                     RVAANIRAQQRYRFRLFEYEVLSSSPS"
     gene            complement(7685..9502)
                     /gene="cysC"
                     /locus_tag="VF547_04535"
     CDS             complement(7685..9502)
                     /gene="cysC"
                     /locus_tag="VF547_04535"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX8262121.1"
                     /translation="MGADESVVPGEAAATGEKTLLRLITCGSVDDGKSTLLGRLLYET
                     GSAFDDELASCGEDLSRLVDGLEAEREQGITIDVAYRFFATDKRKFIVADAPGHEQYT
                     RNLVTGASTAELALVLVDAAKGVLTQTRRHAYLCRLLGVRQFVLLVNKMDSVGHDRAA
                     FERIVADFAAMGGPFTAIPVSALAGDNVTARSPAMPWYEGPTLLEHLEAAEPEGRAEG
                     GFAMPVQWVSREGRRGFAGTIVSGEARRGDRVRVMPAGTPATLARIRLGEAELDLAVQ
                     GQSVMLSFEEEVDCSRGDVVAAAGCPPAVADQFEATLIWMAEDELVAGRQYGLKLGTQ
                     SALATIHTPKYEIDVDTLEQVPAHGLRLNGIGVANLWTDRPLVHAAYEDDRDLGGFIL
                     IDRVSNETVGAGLIRHALRRSHNLHAQALEVTREARASIKGQRPRLLWFTGLSGAGKS
                     TIANLVEKKLHLAGRHTYLLDGDNVRLGLNRDLGFTDADRAENIRRVGETARLMLDAG
                     LIVLAAFISPFRAERRMVRDMLGEADFVEIFVDVPLAEAERRDPKGLYAKARDGRIPN
                     FTGIGSPYEPPEAAEIRIDTTAMTAEQAADEIVRWLERP"
     gene            complement(9502..10500)
                     /gene="cysD"
                     /locus_tag="VF547_04540"
     CDS             complement(9502..10500)
                     /gene="cysD"
                     /locus_tag="VF547_04540"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX8262122.1"
                     /translation="MLAAEEGEGFDKLSPNGDKLDPTRTTSRREPGAMTLTHLQRLEA
                     EAIHVMREAAAECERPVMLYSAGKDSSVLLHLARLAFMPSRPPFPLLHVDTMWKFRAM
                     YELRDRAAAQAGMELIVHRNPEAEERGINPLDHGSALHTQMWKTDGLKQALDRHGFDA
                     AIGGARRDEERVRAKERIFSFRSAGHRWDPKRQRPELWRLYNARKAKGESFRIFPLSN
                     WTELDVWQYVEAQGIEVSELYFAAPRPTLEREGALLMVDDARLKPRPGEEVRERWVRF
                     RTLGCYPLSGAIESRATTVPELIEEMRTGRQSERQGRLIDRDGTASMERKKREGYF"
BASE COUNT         1607 a         3823 c         3548 g         1580 t
ORIGIN      
        1 gaccggctcg accccgacgc cgccgagggc atcgtcgagg ctatccgcgc ctccggccgc
       61 ctccgcctag ggggccgtcg ggagatcggc ttcgacgagc cgcgcgacct gaggttcctc
      121 cagcgcgaac gccttcccca ccattcgaac ggcatgcgct tcaccgcctt cgacgccgcc
      181 ggcgaggcgc tcgaatccgc ggtctcctat tcggtcggcg gcggcgccgt cgtcgacgag
      241 gcggcgatcg cccgcaacgc cccgccggac ggcttctggg acatccccta caattatcat
      301 tgcgccgacg agctgctcgc catttccgac cgcgaaggcc tcaccatcgc cgacatcgcc
      361 cgcgccaacg agcgggccgc cttgcccgac gcggagatcg accgccgact cgatgcgatc
      421 agcgccgcca tgagccagtg catcgaccgc ggcatcggcc gtgacggcat cctgcccggg
      481 ggcctgaagg tgaagcgccg cgccccggcc ctgcacaagc ttctgctcga gcgcgccgag
      541 cgcacgctct cggatccgct gacggtgctc gactgggtca atctgtgggc gctcgcggtg
      601 aacgaggaga atgccgcggg cggccgggtc gtcaccgctc cgaccaacgg cgccgccggc
      661 atcgtcccgg cgatcctgcg ctactaccag cgcttcgtgc cccattcgga ccagcggggg
      721 gtccgcgact tcctgctcac cgccgcggcg atcggctcgc tgttcaagga gaatgcgtcg
      781 atctcgggcg ccgaggtcgg ctgccagggc gaggtgggag tcgcctgctc gatggcggcg
      841 gcgggcctca cggcggcgct cggcggcacg ccctggcaga tcgagaatgc cgccgagatc
      901 ggcatggagc acaatctcgg gctcacctgc gatcctgtcg gcggactcgt gcaggtgccc
      961 tgcatcgaga ggaacgcggt gggcgccatc aaggcgatcg aggcctcgcg cctcgccctg
     1021 gtgggtaccg gcgaccatct cgtcagcctc gaccaggtga tcgagacgat gcgcaagacc
     1081 ggcctcgaca tgaacgagcg ctacaaggag acgtcgctcg gcggcctcgc cgtcaacgtt
     1141 gtcgaatgct gaggcccgtc gtcccagcgg aggctggggc cccggcgggc gaagaatgcc
     1201 gcgacttcgc ccgaggggat gcaggccttc gctgacggtc gggcttgctc cggcgcttcg
     1261 tcgctcctag cgtcccggcc gccttctgcc cgaggagttc ccatgcgcct tcgccacgtc
     1321 ctgctcgccg ccgccgcctt cctcgccgcc ccagcgctcg ccgaggtcga gaagcccgcc
     1381 gcgctggtcg ccgacggcat cccggccgtg cccgacgagc tcgccgagaa gacccgtccc
     1441 tacatggagt tccgctcggc cgggctgcag ggctggaacc cgaagacccg ggccatcgcc
     1501 atcaccaccc gctttggaaa caccgcccag gtccacgagg tcgcccgccc cctcggcatg
     1561 cgccgccagg tcaccttcga ggccgacgcc atcgccggcg cttcctatgc gcgccggatc
     1621 ggcgacgtcc tggtggtcca gaaggacgtc ggcggcagcg agttctggca gctctacgtg
     1681 ctcgagggcg gccggctcaa gctgatcacc gacggcaaga gccgcaacag catcaacaat
     1741 tggagccacg acggccgctg gctcgcctac acctcgaccc gccgcaacgg cgccgacaac
     1801 gacatctgga tcgtcgatcc ccgcaacccg gcgagcaatc gcctgctcgc cgaggtcaag
     1861 ggcggcggct ggggcctcgc ggatttcgct ccggacgggc ggagcgccgt cgtcgccgaa
     1921 tatctctcgg tcaacaagac caacctctac cgagtggaca tcgccaccgg ccggatgacg
     1981 ccgatcggcg atcattccaa gcagatctcc tacggcgcct cccaattcgc ccccaacggc
     2041 accttgtggg tcacctccga cgaaggctcg gacttccagc gcctcggcat cctcaatccg
     2101 acgaccggcc gcttcacagc ggtcgcgccc gagccgcgct gggatgtcga gaatttcgac
     2161 atttcggacg acggccggtt catcgcctac accgtcaacg aggcgggaat cagccgcccc
     2221 aagctgctcg acacccgcac ccgcaagatc cggtcggtcg ccggccttcc ggtcggcacg
     2281 atgggcggcc tggaggtcgc gccctggggc gacgtcgcgg tctcggtgtc gtcggcccgg
     2341 tcgccgtcgg acgtctacgt cgtcaatccc cggacgctcg cggtcacccg gtggacggag
     2401 agcgagaccg gcggcctcga tccgaggcgc aacgtcgagc cggtgctggt cgagacgacc
     2461 agcttcgacg gcgagaaggt ctccggcttc ctctaccggc ccgatccggc ccgcttcccg
     2521 gggcgccggc ccgtgctcgt caacatccac ggcggccccg aaggccagtc gcggccgggc
     2581 ttcctgggcc gcaccaatta ttatctgaac gagctcggga tcgccatctt ctaccccaat
     2641 gttcgaggct cgaccggttt cgggaagcgc ttcgtcgcgc tcgacaacgg ccctgacctg
     2701 cgcgaaaatt cggtgcgcga cataggcgcc ttcctcgaca ggcttgctgc cgatcccggc
     2761 ctcgatccgg cccggatcgg agtcaccggc ggctcctacg gcggctatat gtgctacgcg
     2821 agcgcgatcc attacggaga gcggctcaag ggcgccaatt gcgccgtcgc catctcgaac
     2881 ttcgtcacct tcctcgagaa tacgcagagc taccgccgcg acctgagacg ggtcgaatat
     2941 ggcgacgagc gcgacccggc gcagagaaag aaattgctcg ccatttctcc gctgacccgg
     3001 gtgaaggagc ttcgcctccc cttgatggtc cagaccggcg gcaacgatcc gcgcgtgccg
     3061 gcgtcggaag cggagcaggt gatcaaggcg gtccgccgca acgacggcac cgcctggcac
     3121 ctcctcgccc aggacgaggg ccatggcttc gccaagaagg tcaaccagga ttacgctttc
     3181 tggacgagcc tgatgttctg gcagcgcaac ctgctcggcg agcggcgcta gaccggatcg
     3241 tctagggctg gaccgctcag cccctgctca ggatgagcgg gttggcctga aacaaggcga
     3301 tcatggccta gcgggcaagc agaagcgcga gcgcgtcggc ggccttgtcc ggatccttca
     3361 tcggcaatcc gacgtcgggg cgacgccctc gatctcgttg gtgccgtcct tgaggaagcg
     3421 ggcgcaattg ggcatccgca cgtcgcgcgg gatcgcccgc agccgcgtcc gggtgccgcc
     3481 gtcgacatag ccgcagcccg cgccgaaggt ccgggcgccg atcagcgtgg ccgcgcgatt
     3541 gtccttcatc caggcgacga aatcctctga cgccgaggcg gtgtccccgt cgatcagcac
     3601 cagcaggggc ccgctccacg cgccttcgcc cgccatccgc accggcgggt ccggcttggc
     3661 gaagacggag cagggcggcc gctttccccg ccagaccgag cgccggtcgc aggcgggtcc
     3721 gacccgccgg ggcgccttcc gctcgagcct ccgggcggtg aacagcggca ccgcctcgtc
     3781 gacccattcg gaccctccgc cgttgcggct gagatcgacg atcagccgtt tcgcccccgc
     3841 ccggcgaagc tcgccgaggc ggtcccgcag ctccgcctgg agcgacgcgc gcaccgcaag
     3901 ctgcagcgcc cgctcgctca tgcccgggcg gaaccgggcg gcgcaggccg ccagatattg
     3961 gttctcgccg aactcggcga tgcgcagcat tcccgtcgat ccggtgaggc cggaggggaa
     4021 gttgccggcc gcgagcggcc gccagccggg cagccgctcg accgggaagc ggaaatcgtg
     4081 gcggccttcc cggtagcccg cgccggcgca gtcgggcacg ggcgggtccg cctgcggcgc
     4141 cccttccgcg gcgcggcgat cccggtcggc gagctggaaa tgcgggtcgt cgaaacggtc
     4201 gatgaaccgg ttcagcgccc gcaaggcgat caggtgggaa cccgcattct cgatctcgct
     4261 cttcgtctcg atcgacaacg cggcgaggtc gagcccgcgg cgctcggcga tccagtcgag
     4321 attggcatag ccctgggcca tgtccgcctt cagccggtcg aaatccgcgt tccaggcggc
     4381 ggcgtcgtag gtggccacgt cggcgaaggc ggcgagcgcc gccagtaccg cgacgccgct
     4441 ggcgatcctc gccgcccagg ccccgatcct catttgcgcg gccccttcgc cgccagccat
     4501 tcctcgatgg cgcggtaggc ggagcggccg cggggcgggt cgtcccccag cgccgtcgcg
     4561 aaataggcta tgcctcgtcc cccgttcgca tcgatccaca ggcctgagcg caacccgtag
     4621 gcgtcgccgg catggccgag gcggggcttg ccgtcgccga acgggtcgtc tttcggggcg
     4681 cattgggcga ggatctgcac ggcgagaccg tagccgcagt agaaaccgcc ctccgtgtcg
     4741 ccgttcgagc cgtcgaagcg ccagtggatc cgctggattt ccgccaggct cgactccgag
     4801 aggaatcggc cgccgtccgg caggcggccg cgccgcaaca gcatcccccc gacccgggcg
     4861 aggtcgcgga ccgagatgcg caggccgccc tggggggaga agagggcacc gttgctgccg
     4921 agccggtagt cggacaggtc gcagccgccg ttcgagtccg gcaccaccgg gcagtccggc
     4981 cgcttgccct tgaggtcgtc gcggaccacg gcgccgtcgg gcgcgtagag caccgccgcg
     5041 cgggcgatcc tggcgtcggt gcaggtcgtc cagttgaagc aggcgtcgag cgccaggggc
     5101 ttcagcacca gccgctccat cagccggtcg aaccgttctc ccgccgcccg ctcaaggacc
     5161 gaggcgacga ccggaaagcc caggttggag tagcggaagt aggttcccgg ccgatgctcc
     5221 ttgtcccagg cctccggagc ggcgagcgcc tgctcgacgg tcatcccgag cggaagcgcg
     5281 tagctgatgt cgtcgcgaag gctcgaccgg tgggagagga gcagccgaag cgtgatcggg
     5341 cggtcgggaa aggaaggatt gcgaagcgtc cagcccagat gccgggacac gtcctcgtcg
     5401 aggtcgatct tgccctgctc gaccagccgc atcacgccca aggcgacgtg gagcttggtg
     5461 atcgaggcga tccggaccgg atcgtcgatc gtgagcgccc gccccgaacc gcggtcggcg
     5521 aggccctggg cgcccgaggc ggtgactccg cgcgtgtcga aagccgccca ggcataagtg
     5581 ccggccgggg gcggggacgg ggaaggatcg gcgaccgacg cgcaggcgcc cagagcgagc
     5641 ggcatcagca gggcgagacg gcgcatccga tcatgctagc ggcaaatcgg ccgtttccta
     5701 gccaggattc gcctgccatg gtccgcggcg tgcaaccccc tgtccgcgag gcccgatgaa
     5761 gacgcctgcc cttctcctga tgctgtcgct ggcgctttcg gccgccgccg cgcagccgcg
     5821 gccggcgccc cgcgaatggt cgccggccca ggcccgggcg gtcctcgaca agacccagac
     5881 gattcggctg gcgccgtcgc tcgcccatct ctctcccggc gagcggacgg cggtggcgaa
     5941 gctgctcgag gtcgggcgga ttttccagga cgtctacgag gcgcagcggc atcgcgatgc
     6001 gctcggcgcg cgcgccgccc tctcccggcg ccgcgatccg cacggccgca gcctcgacgc
     6061 cctctaccgc ctcaaccagg gcccgatcgc caccacgctc gacaaccgcc gggagccgtt
     6121 cctcgccgtg ccggaggccc cgcccggcaa gaacgtctat ccgtgggacc tcggcaaggc
     6181 cgagctcgac gccttcgtcg ccgcccatcc ggccgagcgc gcccgcctcg ccgaccttcg
     6241 ctcggtcgtc cgccggggcg agcttgcggc tctgaggcgc gatctcgcca agctgcgcca
     6301 atatccggcg atcgacatgc tccatccggg gctgcgcgcc cgactcgagg cgctggcgag
     6361 gaggccgagc cgcgccgtcc tctacgccgt gccctattcg ctcgcctatg ccgacgagat
     6421 ggtccgcagc caccggcttc tcaacgaggc cgccgacgcg gtcgagccgt cggactggca
     6481 gttcgcccgc tacctgcgca accgggcgcg cgatctcctg tccgacgatt acgaatccgg
     6541 cgacgccgcc tggatcaccg gccggttcag gaacctcaac gcccagatcg gcgcctacga
     6601 gacctatgac gacgagctgc tcggcacgcg cgccttctac ggcctcagcc tgctcgccac
     6661 ccgcaacgcc gaatccgccg cgctcaagga cgcgatgaag gggatgcagg aactcgagaa
     6721 ttcgctcccc acggagcgcc acaagaaggt gatcgaggac atcccggtgg gagtctatga
     6781 cgtcatcgcc gatttcggcc agacccgcgg cggcaacacc gccaccaacc tgcccaacga
     6841 ggcctatctc gccgcccgct acggcagcac gatcctgctt cgcgccaaca tcatgcgcaa
     6901 cccggacatc ttccagggct cgggcgacat ctgggccgcc gcgatggcgc cggccttcgc
     6961 gggccacctc accgcggact ccaatttcta ccggaccctg tggcacgagg tcggccatta
     7021 tctcggaccg gacacgaccg tgtccggcgc gagcctcgac tcgatcggcg ccgactcgaa
     7081 cctgctcgag gagctgaagg cggacctcgt ctccctgttc gtcgcccggg agctgcggaa
     7141 gcgcggctat tatagcgacg agcagctgcg ctcgctctac gccagcggca tcctgaggac
     7201 cctccagaac aacaagccgc gccgcgagca gccctacaac atgatgcagc tcatgcagtg
     7261 gaactggttc ctcgaccgcc gggtgctcag cttcgatccg gcgagctcga agctgagcat
     7321 cgactacgac cgctatcacg aggcggtgcg cgacctgctc aagcaggtca tcgccatcca
     7381 ggaaagcggc gacgccaagg ccgccgccgc cttcatcgac cgctggggga cctgggacga
     7441 caatctccac ggccgcgtcg ccgccaacat ccgcgcccag cagcgctacc gtttccggct
     7501 gttcgaatat gaggtccttt catcctcccc gtcctgaggc ggggagggga ccatgcgaag
     7561 catggtggag gggtaagcag gcggagacgt gccgggttcg aactcgatcc gctgaatccg
     7621 cgccgatcta cccctccacc acccttcggg tggtcccccc tccccacgcg ctgcatcggg
     7681 aggatcaggg gcgctcaagc caccgcacga tctcgtccgc cgcctgttcc gcggtcatcg
     7741 cggtcgtgtc gattcggatc tccgcggcct cgggcggctc gtagggggag ccgattccgg
     7801 tgaagttggg gatccggccg tccctcgcct tggcgtagag gcccttgggg tcgcgccgct
     7861 ccgcctcggc gagcgggacg tcgacgaaga tttcgacgaa atcggcttcg ccgagcatat
     7921 cgcgcaccat cctccgctcg gcccggaacg ggctgatgaa ggcggcgaga acgatcaggc
     7981 cggcgtcgag catcagccgc gcggtctcgc cgacgcggcg gatgttctcg gcgcggtcgg
     8041 cgtcggtgaa gccgaggtcg cggttgaggc cgagccggac gttgtcgccg tcgagcaggt
     8101 aggtgtggcg gccggccagg tggagcttct tctcgacgag gttggcgatg gtcgacttgc
     8161 cggcgcccga aaggccggtg aaccagagca ggcgcggcct ctgtcccttg atcgacgcgc
     8221 gcgcctcgcg cgtcacctcg agcgcctggg cgtggaggtt gtgcgagcgc cgaagcgcgt
     8281 ggcggatcag gccggcgccg accgtctcgt tgctcacccg gtcgatcagg atgaagcccc
     8341 cgaggtcgcg gtcgtcctcg taggcggcgt ggaccagcgg ccggtcggtc cacagattgg
     8401 ccacgccgat tccgttcagc ctgaggccgt gcgcgggcac ctgctcgagc gtgtccacgt
     8461 cgatctcgta tttgggcgtg tggatcgtcg ccagcgcgct ctgcgtgccg agcttcaggc
     8521 cgtattggcg gccggcgacc agctcgtcct ccgccatcca gatcagcgtc gcctcgaact
     8581 ggtcggcgac ggccggcgga cagccggcgg ccgcgaccac gtccccgcgc gagcaatcca
     8641 cctcctcctc gaaggagagc atgaccgact ggccctgcac cgccagatcg agctccgctt
     8701 cgccgagccg gatccgggcg agcgtcgccg gagtcccagc cggcatcacc cgaacccggt
     8761 cgccgcgccg cgcctcgccc gacacgatcg tgccggcgaa gccgcgccgc ccctcgcggc
     8821 tcacccactg gacgggcatc gcgaagccgc cctcggcccg gccttcgggc tccgccgcct
     8881 cgagatgctc gagcagggtc ggcccctcgt accagggcat ggccggcgat cgcgccgtga
     8941 cgttgtcgcc ggcgagggcg gagaccggga tggcggtgaa cggcccgccc atcgcggcaa
     9001 aatcggcgac tatgcgctcg aaggcggcgc gatcgtggcc gaccgagtcc atcttgttca
     9061 ccagcagaac gaactgcctc acccccagca gccggcacag ataggcatgc cgccgggtct
     9121 gggtgagcac gcccttggca gcgtcgacca agaccagcgc cagctccgcg gtcgaggcgc
     9181 cggtgacgag gttgcgggtg tattgctcgt gaccgggagc gtcggcgacg atgaacttgc
     9241 gcttgtcggt cgcgaagaag cggtaggcga cgtcgatggt gatgccctgc tcgcgctccg
     9301 cctcgagccc gtcgacgagg cgcgagagat cctccccgca cgaagcgagc tcgtcgtcga
     9361 aggcggagcc cgtctcgtag agcaggcggc cgagcagggt cgacttgccg tcgtcgaccg
     9421 acccgcaggt gatcaggcga aggagcgtct tttctccagt cgccgcagcc tcccctggga
     9481 cgacggattc gtccgcgccc atcagaagta gccctcgcgc ttcttgcgct ccatcgaggc
     9541 ggtgccgtcc cggtcgatga ggcggccctg gcgctcggac tggcggccgg tgcgcatctc
     9601 ctcgatcagc tcggggaccg tggtcgcgcg gctctcgatc gcgcccgaca aggggtagca
     9661 gcccagggtg cggaaccgca cccagcgctc gcgcacctcc tctccgggcc gcggcttcag
     9721 ccgcgcgtcg tcgaccatca gcagcgcgcc ctcgcgctcg agggtcggcc gcggcgcggc
     9781 gaaatagagc tccgaaacct cgatcccttg cgcttcgaca tattgccaga cgtcgagctc
     9841 ggtccagttg gagaggggaa agatgcggaa gctctcccct tttgccttgc gggcgttgta
     9901 gagccgccac aattcgggcc gctgccgctt cggatcccag cgatggccgg cggagcggaa
     9961 ggagaagatc cgctccttcg cccgaaccct ctcctcgtcc cgccgcgcgc cgccgatggc
    10021 cgcgtcgaag ccgtgcctgt cgagcgcctg cttgaggccg tcggtcttcc acatctgcgt
    10081 gtgcagggcc gagccgtggt cgagcggatt gatgccgcgc tcctcagcct ccgggttgcg
    10141 gtggacgatc agctccatcc ccgcctgggc ggcggcgcgg tcgcgaagct cgtacatggc
    10201 ccggaacttc cacatcgtgt cgacgtggag gagcgggaag ggcggccggg acggcatgaa
    10261 cgccagcctc gcgagatgga gcagcaccga gctgtccttg cccgccgaat agagcatcac
    10321 cggccgctcg cactcggccg ccgcctcgcg catgacgtgg atcgcctctg cctcgagccg
    10381 ctggaggtgg gtgagcgtca tcgctcctgg ctcgcggcgc gatgtcgtgc gggtcggatc
    10441 aagcttgtct ccgttcgggc tgagcttgtc gaagccctct ccttcctccg ccgctaacag
    10501 gaacgaaagc ccttcgacaa gctcagggcg aacgcttgta tcgggtcctg ttgcaact
//
LOCUS       DASVQZ010000161         6473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_133548, whole genome shotgun sequence.
ACCESSION   DASVQZ010000161 DASVQZ010000000
VERSION     DASVQZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133548"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..708
                     /gene="pyrE"
                     /locus_tag="VF547_04545"
     CDS             115..708
                     /gene="pyrE"
                     /locus_tag="VF547_04545"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX8262123.1"
                     /translation="MTEEEILAEFRAAGALLEGHFILSSGLHSARYLQCARVLMDPAR
                     ASRLASSLAHSLPREIRSKVEMVVSPAMGGVIIGHEMGRSLGLEAIFLERPTGTFEAR
                     RGFRIEPGQKALLVEDVVTTGLSSREAMKAVEAAGGEVVAAAALVDRSNGLADLAIPF
                     FPLLRLDVPVYEPEAVPPELARFPAEKPGSRRKAEAA"
     gene            705..1436
                     /locus_tag="VF547_04550"
     CDS             705..1436
                     /locus_tag="VF547_04550"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX8262124.1"
                     /translation="MTGPLRLGVNIDHVATIRNARGGDHPDPVKAALAAAKAGADGIT
                     AHLREDRRHIRDEDIAALTGQLTIPLNLEMAATEEMLEIALRHRPHAACLVPEKREER
                     TTEGGLDAAGQDNHLLPYVARLAGANIRVSLFIEPDPRQIEAAIRLKVPVVEFHTGRY
                     AHLEGEARAAELARLADAAALAAKNGIEPHAGHGLTFDNVQPIAAIPQLRELNIGHFL
                     VGEAIFTGLGESVARMRALMDEARG"
     gene            1576..1992
                     /gene="acpS"
                     /locus_tag="VF547_04555"
     CDS             1576..1992
                     /gene="acpS"
                     /locus_tag="VF547_04555"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX8262125.1"
                     /translation="MIIGIGSDLCNIERIERSLARFGERFVNRVFTEAERARAEGRPL
                     TRAGTYAKRFAAKEAFSKAVGTGFKRGVFMRDIGVVNLPSGAPTLALAGGARARLDAL
                     TPEGHLAQIHLTMTDDYPFAQAFVIITASPLPRSPG"
     gene            1989..2882
                     /gene="lepB"
                     /locus_tag="VF547_04560"
     CDS             1989..2882
                     /gene="lepB"
                     /locus_tag="VF547_04560"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8262126.1"
                     /translation="MSSSDPAMTDTPEARPDTPAPAPAGKKGGTDWWAELKAIFWLIL
                     AVLAFHSFIAKPFYIPSESMMPGLLKGDRLVVSKYPYGWSWVSPSFHILPHWQGRLFG
                     RMPERGDVVILTPRGKTSDYIKRVVGLPGDTLEMSGGTLFINGRAVPREARPPVRVAV
                     DPNAPCDRDLVSRQVMGADGKLYCELPVYRETLPNGRSYDTIDLGPSDVDDFPRITVP
                     PGHVWVQGDNRDKSADSRVPARSEGLDGPVPWENIGGRAEFITFSLDGSSSYLNPISW
                     ITAMRGGRAGTSLRPQEGKAP"
     gene            2990..4003
                     /locus_tag="VF547_04565"
     CDS             2990..4003
                     /locus_tag="VF547_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8262127.1"
                     /translation="MRDPFVREELKRASVWFGLALAIAAVIFLAQPLLLIFAGIVLAS
                     MLDGGTRLLGRVLPIGRGWRLAIVTLAAVGFIVWVFYFAGTELAGQAERLREVITAQG
                     NRVLAWGNSLGLVQGGVGVDQLGGQLMGTLGRLGSAVSSALGVVASTVMILVIGIFIA
                     VEPRLYQRGVAWMLPLGSRERFYSTAEQMGFTLRRLMAGRLVGMAVEGIGTWILLALG
                     GVPMAALLGILTGLLAFIPNIGAIVSGVLIVLAGFSVSVNAGLWAIAVYFIVQTVDGY
                     LIVPYVARKTVDLAPALVLGAQLLFGALFGLMGLMLADPIVAMLKVALERKSIDDEGE
                     SPA"
     gene            complement(4143..4724)
                     /locus_tag="VF547_04570"
     CDS             complement(4143..4724)
                     /locus_tag="VF547_04570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8262128.1"
                     /translation="MSVTAVPLQPIRKGSVAKLWIALAVLAALAAAFAWWGTQAFQPV
                     TTQNGVGIRTLRAGSGAKITPQDVVALHYKLHAKSLDAPVVQDSRETGQPFVTTTQGV
                     YPGFAEGLQHMRAGGSYVLTLPPGTHEQGPIPPGAPFTAKDSLVFEIDVLQVEAGAGP
                     RFLEMQRMQQLQQMQQMQQMQGAPPEGPPEGAR"
     gene            complement(4861..5076)
                     /gene="rpsU"
                     /locus_tag="VF547_04575"
     CDS             complement(4861..5076)
                     /gene="rpsU"
                     /locus_tag="VF547_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX8262129.1"
                     /translation="MQIIVRDNNVDQALRALKKKLQREGVYREMKLRRHYEKPSEKRA
                     RERAAAIRRARKLERKRAERDGGGTTR"
     gene            5344..5847
                     /locus_tag="VF547_04580"
     CDS             5344..5847
                     /locus_tag="VF547_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262130.1"
                     /translation="MSEPYALPARLLELLQDMPLEELAFTPVPVRARYDGWAVERQKW
                     FVLRLALGGCVSVAAKGVGMTRKSAYRLRDRPGAESFAAAWDRAQGWGRDRTVDVSLE
                     RSLLGERVPIVRNGRVVGEVVRHDNRLAMAVLNALDRRAAARPRIAALEAALSRLEET
                     SGKLRLY"
     gene            complement(5902..>6473)
                     /locus_tag="VF547_04585"
     CDS             complement(5902..>6473)
                     /locus_tag="VF547_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8262131.1"
                     /translation="FLRAELPRLAPVRRLVDVGGGSGVGAIVAAGVLPGARVTVTDVN
                     PLALRFARIAARQAGVALETVEAPGLDGVEGPVDLVVANPPFVADPAGRLYRDGGGMR
                     GASLSLDWALAAARRIEPGGTVLLYTGSAIVAGRDALHEALAQRLPALGCTLRYAEID
                     PDIFGELLDQPGYEDVERVAAVGALISKG"
BASE COUNT          920 a         2328 c         2216 g         1009 t
ORIGIN      
        1 agcggatagg cccgatcgcg cgccttatag gcaggcgaac cgcgcgtctc aagcaacttg
       61 atggcggtcg ctgccgggcc tatctgcgaa tacgggccaa tcgggggagc cgcgatgacg
      121 gaagaagaaa ttctggcgga gtttcgcgcc gccggggcgt tgctcgaggg gcatttcatc
      181 ctctcctccg gcctccacag cgcccgttac ctgcaatgcg cgcgcgtctt gatggatcct
      241 gcccgggctt cccgcctcgc ttcctcgctc gcgcattcgc ttccgcgcga gatcaggtcg
      301 aaggtcgaga tggtcgtgtc gccggcaatg ggcggtgtca tcatcggcca cgaaatgggc
      361 cgctcgctcg ggctcgaggc gatcttcctc gagcgcccca ccggcacctt cgaggcccgt
      421 cgcggcttcc gcatcgagcc ggggcagaag gcgctgctcg tcgaggacgt cgtcaccacc
      481 ggcctcagct cgcgggaggc gatgaaggcc gtggaggcgg cgggcggcga ggtggtcgcg
      541 gccgccgcgc tcgtcgaccg ctcgaacggc ctcgccgatc tcgcaatccc cttcttcccg
      601 ctgctgcggc tcgacgtacc agtctacgag ccggaggcgg tcccgccgga gctcgcgcga
      661 ttcccggccg agaagccggg cagccgcagg aaagccgagg cggcgtgacc gggccgcttc
      721 gcctcggggt gaatatcgac catgtcgcga cgatccggaa cgcgcgcggc ggcgaccatc
      781 ccgaccccgt gaaggccgcg ctcgcggccg ccaaggccgg cgccgacggc atcacggcgc
      841 atcttcgcga agaccggcgg cacatacgcg acgaggacat cgcggcgctc accggccagc
      901 tcaccatccc gctcaaccta gaaatggccg cgaccgagga gatgctcgag atcgcgctcc
      961 gccatcgccc ccacgccgcc tgcctcgtcc ccgagaagcg cgaggagcgg accaccgaag
     1021 gcgggctcga cgccgccggc caggacaatc acctgctccc ctacgtcgcc cgactggccg
     1081 gcgccaacat ccgggtcagc ctgttcatcg agcccgatcc gcgccagatc gaggcggcga
     1141 tccggctgaa ggtgccggtg gtcgagttcc ataccggccg ctacgcccat ctcgagggcg
     1201 aggcccgcgc cgccgagctc gcccggctcg ccgacgccgc cgccctggcc gccaagaacg
     1261 gcatcgagcc ccatgccggc cacggcctca ccttcgacaa cgtccagccg atcgccgcca
     1321 tcccccagct cagggagctc aacatcggcc acttcctcgt cggcgaggcg atcttcaccg
     1381 gcttgggcga gagcgtcgcg aggatgcgcg ccctgatgga cgaggcgagg ggatgagcga
     1441 cctgccaagg aagccccctc tccccttgag gggagaggtc cggggagagg ggcagtcggt
     1501 cgacctcgac gtcccttccc ctctcctccc gccgcttcgc ggctccttcc tctcccctga
     1561 aggggagagg cgcccatgat catcggcatc ggctcggacc tgtgcaacat cgagcggatc
     1621 gagcgctcgc tcgcccgctt cggcgagcgc ttcgtcaacc gggtcttcac cgaggccgag
     1681 cgcgccaggg cggaaggccg cccgctgacg cgcgccggga cctacgccaa gcgcttcgcc
     1741 gccaaggagg ccttctcgaa ggcggtgggc accggcttca agcgcggcgt cttcatgcgc
     1801 gacatcggcg tcgtcaacct cccctcgggc gcccccaccc tggccttggc cggcggcgcc
     1861 cgggcaaggc ttgacgcttt gactccggag ggccacctcg cgcagatcca tctgacgatg
     1921 acggacgact atcctttcgc tcaggccttc gtcatcatca ccgccagccc attgccaagg
     1981 agcccagggt gagcagcagc gatcccgcca tgaccgacac gcccgaagcc cggccggaca
     2041 cgcccgcgcc cgcgcccgcg gggaagaagg gcggcaccga ctggtgggcc gagctgaagg
     2101 ccatcttctg gctgatcctc gccgtcctcg ccttccacag cttcatcgcc aagcccttct
     2161 acatcccgtc ggaatcgatg atgccggggc tgctcaaggg cgaccgcctc gtcgtcagca
     2221 aatatcccta cggctggtcc tgggtgtcgc cgagcttcca catcctgccg cactggcagg
     2281 gccggctgtt cggccggatg cccgagcgcg gggacgtcgt catcctgacc ccgcgcggca
     2341 agacgtccga ctacatcaag cgggtcgtcg ggctgcccgg cgacaccctc gagatgtccg
     2401 gcggaacgct gttcatcaac ggccgcgcgg tcccgcgcga ggcccggccg ccggtgcggg
     2461 tcgcggtcga ccccaacgct ccctgcgacc gcgacctcgt ctcccgccag gtgatgggcg
     2521 ccgacggcaa gctctattgc gagcttcccg tctaccgcga gacgttgccc aacggccggt
     2581 cctacgacac gatcgacctc ggcccgtccg acgtcgacga tttcccgagg atcaccgttc
     2641 cgcccggcca tgtctgggtg cagggcgaca atcgcgacaa gtcggccgac agccgggtcc
     2701 ccgcccgctc cgaagggctg gacgggccgg ttccgtggga gaatatcggc ggccgcgccg
     2761 agttcatcac cttctcgctc gacggctcct cctcctatct caacccgatc agctggatca
     2821 cggcgatgcg cggcgggcgc gcgggcacct cgctccggcc gcaggagggc aaggcgccgt
     2881 gagcggcgac gcggcgccct ccgccggccc gccttccgac gcgccggcga tcgcccgggc
     2941 ttcgtccgac agcgaggagc gtcacatcga gcagccgggt cccgccgaga tgcgcgaccc
     3001 gttcgtgcgc gaggagctga agcgcgccag cgtctggttc ggcctcgccc tcgccatcgc
     3061 cgcggtcatc ttcctggccc agccgctgct cctgatcttc gccggcatcg tcctcgcctc
     3121 gatgctcgac ggcggcaccc gcctgctcgg ccgggtgctg ccgatcggcc gcggctggcg
     3181 gctcgccatc gtgaccctgg ccgcggtcgg cttcatcgtc tgggtcttct acttcgccgg
     3241 caccgagctc gccggccagg ccgagcgcct gcgcgaggtc atcacggccc agggcaaccg
     3301 ggtgctcgcc tggggcaaca gcctcggcct ggtccagggc ggcgtcgggg tcgaccagct
     3361 cggcggccag ctcatgggga ccctcggccg gctcggctcg gcggtgtcga gcgcgctcgg
     3421 agtggtcgcc tcgaccgtga tgatcctcgt catcggcatc ttcatcgccg tcgagccccg
     3481 cctctaccag cgcggggtcg cctggatgct gccgctcggc agccgcgagc gcttctacag
     3541 caccgccgag cagatgggct tcacccttcg gcggctgatg gccggacggc tggtcgggat
     3601 ggcggtcgag ggcatcggca cctggatcct gctcgcgctg ggcggcgtgc cgatggcggc
     3661 gctgctcggc atcctcaccg ggctgctcgc cttcatcccc aacatcggag cgatcgtctc
     3721 gggcgtgctg atcgtcctcg ccggcttcag cgtcagcgtc aacgcgggcc tgtgggcgat
     3781 cgccgtctac ttcatcgtcc agaccgtcga cggctatctg atcgtgccct acgtggcgag
     3841 gaagacggtg gacctcgcgc cggcgctcgt gctcggcgcc cagctcctgt tcggggcgct
     3901 gttcggcctg atggggctga tgctcgccga tccgatcgtc gccatgctca aggtcgccct
     3961 cgagcggaaa tcgatcgacg acgaaggcga gtctccggcc tgacgactaa ttcctcccct
     4021 gcatttgcag gggaggggga ccaggcgaag cctggtggag gggcctcact gagtccgtag
     4081 aacccctcca ccatgctgcg catggtcccc ctccccgcga aatcgcgggg aggaatctcg
     4141 gctcagcgcg cgccctccgg cgggccctcc ggcggcgcgc cctgcatctg ctgcatctgc
     4201 tgcatctgct ggagctgctg catgcgctgc atctccagga agcgcgggcc ggccccggcc
     4261 tcgacctgga ggacgtcgat ctcgaagacg aggctgtcct tggcggtgaa cggggcgccg
     4321 ggcgggatcg gcccctgctc gtgggtgccg ggcggcaggg tcaggacgta ggagccgccc
     4381 gcgcgcatgt gctgcagccc ttcggcgaag cccggataga cgccctgggt ggtcgtcacg
     4441 aagggctggc cggtctcgcg gctgtcctgg acgaccggag cgtcgaggct cttggcgtga
     4501 agcttgtagt ggagcgcgac gacgtcctga ggcgtgatct tcgcccccga tccggcgcgc
     4561 agcgtccgga ttccgacgcc gttctgggtg gtcaccggct gaaaggcctg ggttccccac
     4621 caggcgaagg cggcggcgag cgccgccagg acggcgaggg cgatccagag cttggcgacc
     4681 gagcccttcc tgatcggctg cagcggcacg gcggtgaccg acatgttcta tccccgtaga
     4741 aaagcgaagg gcggcgatac atcgccgccc tggtcgggga cagtcacttt tttccggtcg
     4801 ccgacgcgtt cgcggcttcg gccgagggga aaaagcgatt gtcccccttt cggtcccttt
     4861 ttagcgggtc gtcccgccgc cgtcccgctc ggcgcgcttg cgctcgagct tgcgggcgcg
     4921 gcggatggcc gcggcccgct cgcgggcccg cttctccgac ggcttctcgt agtgacggcg
     4981 cagcttcatc tcgcgataga cgccctcgcg ctgcagcttc ttcttcagcg cccgcagcgc
     5041 ctggtcgaca ttgttgtcgc gaacgatgat ttgcataaag gctcgtcact ctccgtcgaa
     5101 aggcatttcc gggcaacctc ctgggccgcc catcgcggaa acgccgatag cataaaaatc
     5161 ggttcgtcgc gagccactac ccgcgccgtc acgccggggc cgttagcagg cgccgcgctg
     5221 ggtttcaagc cgttaggggg ggcttgccac ggtcgagatc gatccgcccg cctccgcccg
     5281 cttcggaagg ttggaacaga aaaggaacta gtcaagtcgt gatcgaacat atatagtacg
     5341 cagatgtccg agccctacgc cctgccggct cgcctgctcg agcttctgca ggacatgccg
     5401 ttggaggagc tcgccttcac gcccgtgccg gtcagggccc ggtacgacgg ctgggcggtg
     5461 gagcggcaga aatggttcgt cctccggttg gcgctgggcg gctgcgtcag cgttgcggcg
     5521 aagggagtcg ggatgacccg caagagcgcc tatcgccttc gcgaccgccc cggcgcggag
     5581 agcttcgccg ccgcctggga ccgggcgcaa ggctggggcc gggaccgcac cgtcgacgtc
     5641 agcctcgagc gcagcctgct gggcgaacgg gtgccgatcg tgcgcaacgg ccgggtcgtc
     5701 ggcgaggtcg tgcggcacga caaccggctg gcgatggcgg tgctcaatgc gctcgaccgg
     5761 cgcgcggcgg cccggccccg gatcgcggcc ctggaagcgg cgctgtcccg tctggaggag
     5821 acttcgggaa aattgaggct gtactgaaaa caaaggattc atcgggtcga catgcgtgtc
     5881 ttttgggtct tttggagccg ctcagccttt gctgatgagg gcgcccacgg ccgccacgcg
     5941 ctcgacatcc tcatagcccg gctggtcgag cagctcgccg aatatgtccg ggtctatttc
     6001 ggcgtagcgc agagtgcagc cgagcgccgg cagcctttgt gccagggcct cgtggagcgc
     6061 gtcgcggccc gcgacgatcg cgctgccggt gtagagcagg accgtgccgc cgggctcgat
     6121 ccgccgcgcc gcggcgagcg cccagtccag cgacagcgac gcgccgcgca ttccgccgcc
     6181 gtcgcggtag agccggccgg ccgggtcggc gacgaagggg ggattggcga cgaccaggtc
     6241 gaccgggccc tcgacgccgt cgaggcccgg cgcctcgacg gtctccagcg cgacgccggc
     6301 ctggcgggcg gcgattcggg cgaagcgcag cgccagcggg ttgacgtcgg tgaccgtgac
     6361 tcgcgctccg ggcagcacgc cggccgcgac gatcgcgccg actccgctgc cgccgccgac
     6421 gtcgaccagg cgccggaccg gcgcgagtcg aggcagctcg gcgcggagga agc
//
LOCUS       DASVQZ010000162         7649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_229149, whole genome shotgun sequence.
ACCESSION   DASVQZ010000162 DASVQZ010000000
VERSION     DASVQZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229149"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VF547_04590"
     CDS             <1..521
                     /locus_tag="VF547_04590"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX8262132.1"
                     /translation="LWFDMLIHNPLWTEQKVGAWPVLNLLAPAYLLAAYWLYRARRGA
                     DGALRSGAWLLLSLASLVLGAMLMVRQAFQGPLLNGPQVPSAESYGYSLVGLLLSIAL
                     LLAGIRLPDKALRLAGLVFLTATTVKVFLVDAGALEGVLRILSFLVLGIALIGIGKLY
                     TKVLDAEARPSG"
     gene            518..1645
                     /locus_tag="VF547_04595"
     CDS             518..1645
                     /locus_tag="VF547_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8262133.1"
                     /translation="MTLRVLLLAAALAACGPPAASSGQAEPRAGAAAAIPMPVARAAH
                     GAAALPDGRVLLVGGCVLESCEAGPESATVDAYDPRTGRFAHAGRLLERRVSAAVLLL
                     ASGEILIAGGWAGRAVSDRVELLDPRTGRSRPGPPLRLARSDLAAAVLADGRVLLAGG
                     FDGIGSADLVEIFDPATGALERIGRLAVARAGAGAAVLRDGSVLVVGGGTGPGAREPT
                     AAAEIFDPRLGRSSRTGGLAEARYKHATVPLGDGRVLVLGGSDRRDSRGKLASVERYH
                     PARRAFAAAGRLVEPRYKIAGSVLRLADGKVLVAGGAPRAELYDPASGRSEPTGPALG
                     KSLNFATATLMADGSALIAGGYEENGIEMNREAWRLPPAPR"
     gene            complement(1673..1749)
                     /locus_tag="VF547_04600"
     tRNA            complement(1673..1749)
                     /locus_tag="VF547_04600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1713..1715),aa:Pro,seq:ggg)
     gene            2098..2577
                     /locus_tag="VF547_04605"
     CDS             2098..2577
                     /locus_tag="VF547_04605"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8262134.1"
                     /translation="MGIERRFGVASRDEIAGLSGLEMLEAIIAGRLPSPPIGAAMGFR
                     LVEVEPGRALFEGEPGPHLLNPLGGVHGGVALTLIDSAAGCAVHTELPPGVGYTTVET
                     KVNFTRPIAPDGGTVRCEGRVVTRGRQIATAEARLLSAEGKILAHGTSTLIILQPRG"
     gene            complement(2610..2966)
                     /locus_tag="VF547_04610"
     CDS             complement(2610..2966)
                     /locus_tag="VF547_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262135.1"
                     /translation="MRKAFALAAAAALVGLPGPASAAPDGKNRTVTVQNVSNQTVREL
                     YASPITAKTWEEDLLGQRTLTAGQSISANIDNGTTECYYDLKAVLANGKAVEQRKVNV
                     CAASKWVIGDSGHSIQ"
     gene            complement(3034..3342)
                     /locus_tag="VF547_04615"
     CDS             complement(3034..3342)
                     /locus_tag="VF547_04615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX8262136.1"
                     /translation="MIIVNGTVRFGAGEIDRLRDAMARNVEATRAEDGCEHYAYAVDI
                     SDPDILHVAERWRDDSAIEAHMASPHMGAFMAEIGASRVEAISIKAYEASFLRNVLGE
                     "
     gene            3480..3818
                     /gene="yajC"
                     /locus_tag="VF547_04620"
     CDS             3480..3818
                     /gene="yajC"
                     /locus_tag="VF547_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX8262137.1"
                     /translation="MFSSPAYASAGAAASSGGMAGLFVQFAPLLFIFVIFYFLLIRPQ
                     SRRVKEHQNMIAAVKRNDTAVTSGGLIGKVTKVEEAEIELEVAAGTRVRVVKSMLSEV
                     RPHGAKPAND"
     gene            3824..5398
                     /gene="secD"
                     /locus_tag="VF547_04625"
     CDS             3824..5398
                     /gene="secD"
                     /locus_tag="VF547_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX8262138.1"
                     /translation="MLDFPRWKMWAVSLLCLVGIALAVPSFFPESQVAKWPAFVPSAR
                     INLGLDLAGGSHLLLEADTSDVAKQRLDQMEDIMRSELRRASPRIEVGDMSTAGGRLS
                     FLVRDPARVDAVVDIARNQTQPVALGQRTFDVSVVDGTRVVMTPTQSGLAAALESAMA
                     TAREVVYNRVDPEGTREVTVVRQGKDRILVQVPGLQDPEGLKALLGKTARLEFKLVAA
                     DQSGVQTGRAAPGTQILPMQGGGRIAVVRRAMVNGDQLVDAQQQFDENNEPVVSIRFD
                     SRGSRSFGKVTQENVNKPFAIILDDVVLSAPNINEPILGGQARISGSFTVESATDLAV
                     QLRSGQLPVDLRVIEERTVGPDLGRDSIRSAVIAGIVGTVGILLFMWLTYGRFGLYAN
                     VALILNVIVILGIMAPFATLTLPGIAGFVLTVGAAVDANVIINERIREEHRRGRRVME
                     AVEHGYNEARTAIFDANITNVIAGLLLYYFGSGPVKGFAVVMMIGIATSVFTAVTVTR
                     MFVSLWLRKRPTELRI"
     gene            5407..6375
                     /gene="secF"
                     /locus_tag="VF547_04630"
     CDS             5407..6375
                     /gene="secF"
                     /locus_tag="VF547_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX8262139.1"
                     /translation="MRLLKLIPEDTNLDFLRWRNVAIVLSALLMAASIALIAVKGLNF
                     GVDFAGGQVIRTTFAQPPSLDRLRTEITGLGLGDPSVQEFGSPREISIRLPLPQGGEE
                     GANAAATKVRQALQQRYPGVRIDAVDTVSGKVSEELVWDGALALLLSAIGIVIYIWIR
                     FEWQFGVGALFSLFHDVTLTLGFFALTQMEFDLNVVAALLTLIGYSLNDTIVIYDRVR
                     ENLRKYRKMDIVSLLNLSMNETLSRTVATNFAMLLALGSLMILGPNVIQSFTTALLIS
                     VIVGTYSTVYIAAPWLVWLKVDSNSFLPKTNAASAGERVTSLNEGV"
     gene            6377..6751
                     /locus_tag="VF547_04635"
     CDS             6377..6751
                     /locus_tag="VF547_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEX8262140.1"
                     /translation="MARLDRDAGADGPVVKGFANGGFVVGESVYRTLQLTPLRAEEWA
                     APPVAQLAEADLDTLLALDPAPEFLLLGTGPRLSHPPRALREALERRGIGLEVMDSRA
                     AARVWGLLRGEERWIVAALMPL"
     gene            complement(6899..>7649)
                     /locus_tag="VF547_04640"
     CDS             complement(6899..>7649)
                     /locus_tag="VF547_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8262141.1"
                     /translation="IKARGSDIHYWGRRPAIAARMLEAARRAGGLLAVSAALRLDMAA
                     MGMPEDRIRVHHTGVELDRFRPLDRPAAKRALGVAGPLIVAAGNLVPLKGHRLAVEAA
                     ALLPGATLLIAGEGPERAALEALIAKLGLGSRVRLLGGVAPEAMPELLGAADLMLLPS
                     EREGLANVWVEALACGTPILIADVGGAREVLDRPAAGRLVARDPAAIAAAALEMLAAP
                     PDPAAVRRAAERFSWERNAEELHQHLAGLVR"
BASE COUNT         1083 a         2790 c         2589 g         1187 t
ORIGIN      
        1 tgctgtggtt cgacatgctg atccacaatc cgttgtggac cgagcagaag gtcggggcct
       61 ggccggtcct caacctgctc gcgccggcct atctgctggc cgcctactgg ctctaccgcg
      121 cgcgccgagg cgccgacggc gcgctccggt cgggggcgtg gctgctcctg tccctggcct
      181 cgctggtgct gggggcgatg ctgatggtcc ggcaggcctt ccaggggccg ctgctgaacg
      241 ggccccaggt gccctcggcc gaatcctacg gctattcgct cgttgggctc ttgctctcga
      301 tcgcgctgct cctcgccggc atccgcctgc ccgacaaggc cttgcgcctc gccggcctcg
      361 tcttcctcac ggcgaccacg gtgaaggtgt tcctcgtcga cgccggagcg ctcgaaggag
      421 tgctgcgaat cctttccttc ctcgtcctcg ggatcgccct gatcgggatc ggcaagctct
      481 acaccaaggt gctcgacgcc gaggcccggc cgtccggatg acgcttcgag tcctgctgct
      541 cgccgcggct ctggccgcct gcggcccgcc ggcggcctcg tccggccagg ccgagcctcg
      601 agccggagcc gcagccgcga tcccgatgcc ggtggcgcgc gccgcgcacg gcgccgccgc
      661 cctgccggac ggccgcgtgc tcctcgtcgg cggctgcgtg ctcgagagct gcgaggccgg
      721 gccggaaagc gccaccgtcg acgcctacga cccgcgcacc ggccgattcg cgcacgccgg
      781 ccggctgctc gagcggcgag tgagcgcggc cgtgctgctt ctggcctcgg gggagatcct
      841 gatcgccggc ggctgggccg ggcgggcggt gtcggaccgg gtcgagctct tggacccgcg
      901 gacgggccgg tcgcgccccg gcccgccgct ccgcctcgcc cgctcggacc tcgccgcggc
      961 ggtcctggcg gacgggcggg tgctgctggc gggaggcttc gacgggatcg gctcggccga
     1021 cctcgtcgag attttcgacc ccgccaccgg cgcgttggag cgcatcggcc gcctcgccgt
     1081 cgcccgggcc ggagccggcg cggccgtgct tcgcgacgga agcgtgctcg tcgtcggggg
     1141 cggcaccggc ccgggcgcgc gcgagcccac ggcggccgcg gagattttcg acccccggct
     1201 gggccgctcc tctcgaacgg gcggacttgc cgaagcccgg tacaagcatg ccaccgtccc
     1261 gctcggagac gggcgggtcc tcgtcctggg aggctcggat cgaagggata gccgcggcaa
     1321 gctggcttcg gtcgagcgct accatccggc caggcgcgcg ttcgccgcgg cggggcggct
     1381 ggtcgagccg cgctacaaga tcgcgggttc ggtgctgcgg cttgccgacg ggaaggtgct
     1441 ggtcgccggc ggggcgccgc gggccgagct gtacgacccc gccagcgggc gttcggagcc
     1501 gaccggtccc gccctgggca agagcctcaa cttcgcgacc gcgaccctga tggcggacgg
     1561 aagcgcgctc atcgccggcg gctatgagga gaacggcatc gagatgaacc gcgaggcgtg
     1621 gcgcctgccg ccggcgccgc ggtgaagccc cggggccccc gaagggcaat gatggtcggg
     1681 gcgactggat tcgaaccagc gacccccaca cccccagtgt gatgcgctac caggctgcgc
     1741 tacgccccga ccgaggcgcg cgacttaggc gctggcgggt gcgaaggcaa gcgccgccgc
     1801 ctccgcgcat cgggtgatcc agtctcgatg gacgcaggcc aatcattttt tgagagaagg
     1861 cgcgaaggcg cgaaggcacg aagccaaggc cggcgccagc gcgcatccgc agtcgactgc
     1921 cgagaggtcg gttcaagctc gcctgcggcg agaagggcaa ggcctcgctt cgcgcctcgc
     1981 gccttcgtgc cttcgtgccc cctcgaaatg agtccgcacg gccgacgtca agccggcggt
     2041 tgacggcggc agcgcatcca tctagtttca aatagaaact gataaatgga ggcccgcatg
     2101 ggtatcgaga ggcgcttcgg cgtcgcctcg cgcgacgaga ttgccggcct ttccggcctg
     2161 gagatgctgg aggcgatcat cgccgggcgg ctgccctcgc cgccgatcgg cgcggcgatg
     2221 gggttcaggc tggtcgaggt cgagcccggg cgggccctgt tcgaggggga accggggccg
     2281 cacctgctca atccgctcgg cggggtccat ggcggagtcg ccctgacgct gatcgacagc
     2341 gccgccggct gcgccgtcca caccgagctg ccgcccggtg tcggctacac cacggtcgag
     2401 accaaggtga acttcacccg gccgatcgcg cccgacggcg gcaccgtccg ttgcgagggg
     2461 cgggtggtga cccggggccg gcagatcgcc accgccgagg cgcggctgct gtcggccgag
     2521 ggcaagatcc tggcccacgg cacctcgacg ctgatcatat tgcagccgcg gggatgatca
     2581 cccgcccccc gccagatgcg gaggggcgcc tattggatcg agtggcccga gtcgccgatc
     2641 acccatttgc tggcggcgca gacgttgacc ttgcgctgct cgacggcctt gccgttggcc
     2701 agcaccgcct tgaggtcgta atagcattcg gtagtgccgt tatcgatgtt ggcgctgatg
     2761 ctctggcccg cggtcagggt ccgctggccg agcaggtcct cttcccaggt cttggccgtg
     2821 atcggcgaag cgtagagctc gcggaccgtc tgattgctca cgttctgcac cgtgaccgtg
     2881 cggttcttgc cgtccggcgc ggcgctcgcg ggtcccggca gcccgaccag ggccgcggcg
     2941 gcggcaagcg cgaaagcctt cctcatcacg acctccccgt ccatctcggc cgccatttag
     3001 gtccgcgacg ggcgcaagcg caagcggcgc ggctcattcg ccgagcacgt tcctcaggaa
     3061 gctcgcctca taggccttga tcgagatcgc ctcgaccctg gaggcgccga tctcggccat
     3121 gaaggcgccc atgtggggcg aggccatgtg cgcctcgatc gcggaatcgt cgcgccagcg
     3181 ctcggcgacg tggaggatgt ccggatcgga gatgtcgacc gcataggcgt aatgctcgca
     3241 gccgtcctcc gcccgggtcg cctcgacatt gcgcgccatc gcgtcccgaa gccggtcgat
     3301 ctcgcccgcg ccgaagcgga cggtgccgtt gacgatgatc atcccgaatc tcctcccaga
     3361 gccaggcggc ggattctagg tcggcggcgc gcttgcgcaa gccgcttgcc ccatgttagt
     3421 cgccgcgcgc tttctccgga cgcttgcgtt cgcgccggcc cctcaccagg taacgctcca
     3481 tgttctcatc acccgcctac gcctccgccg gcgccgccgc ctcctcggga ggaatggccg
     3541 gcctcttcgt gcagttcgcg ccgctgctct tcatcttcgt catcttctac ttcctgctga
     3601 tccggcccca gagccggcgg gtgaaggagc atcagaacat gatcgccgcg gtgaagcgca
     3661 acgacacggc ggtcaccagc ggcggcctga tcggcaaggt gaccaaggtc gaggaggccg
     3721 agatcgagct ggaggtcgcc gccgggaccc gggtccgggt cgtcaagtcg atgctcagcg
     3781 aagtcaggcc gcacggcgcc aagccggcga acgactgacc gccatgctcg actttcctcg
     3841 ctggaagatg tgggccgtca gcctcctgtg cctggtcggc atcgcgctcg cggttccaag
     3901 cttcttcccc gaatcccagg tcgccaaatg gccggccttc gtgcccagcg cccggatcaa
     3961 cctcggcctc gacctcgccg gcggcagcca tcttctgctc gaggcggaca cgagcgacgt
     4021 cgccaagcag cggctcgacc agatggaaga catcatgcgc agcgagctgc gccgagccag
     4081 cccgcgaatc gaggtcggcg acatgtcgac ggcgggcggg cggctgtcct tcctggtccg
     4141 cgatccggcc cgggtcgacg cggtcgtcga catcgcccgc aaccagaccc agccggtcgc
     4201 cctcggccag cggaccttcg acgtcagcgt cgtcgacggc acccgcgtgg tgatgactcc
     4261 gacccagtcc ggcctcgccg cggcgctcga aagcgcgatg gcgacggccc gcgaggtggt
     4321 ctacaaccgg gtcgatccgg aaggcaccag ggaagtcacc gtcgtccgcc agggcaagga
     4381 ccggatcctc gtccaggtcc ccggcctgca ggacccggag gggctgaagg ccctgctcgg
     4441 caagaccgcc cggctcgagt tcaagctcgt cgcggcggac cagtccggcg tccagaccgg
     4501 ccgcgcggcg ccggggacgc agatcctgcc gatgcagggc ggcggccgga tcgccgtcgt
     4561 ccgccgagcg atggtcaacg gcgaccagct ggtcgacgcc cagcagcagt tcgacgagaa
     4621 caacgagccg gtggtgagca tccgcttcga cagccgcggc tcgcggtcgt tcggcaaggt
     4681 gacccaggag aacgtcaaca agcccttcgc catcatcctc gacgacgtcg tcctctccgc
     4741 gccgaacatc aacgagccga tcctcggcgg ccaggcgcgg atctcgggca gcttcacggt
     4801 cgagagcgcg accgacctcg cggtccagct tcgctcgggc cagctgccgg tcgacctcag
     4861 ggtcatcgag gagcggacgg tcgggcccga cctcggccgg gactcgatcc gaagcgccgt
     4921 catcgccggg atcgtcggaa ccgtcggcat cctgctcttc atgtggctga cctacggccg
     4981 cttcggcctc tacgcgaacg tcgctctgat cctgaacgtg atcgtcatcc tcggcatcat
     5041 ggcgccgttc gcgaccttga ccctgcccgg catcgccggc ttcgtcctca ccgtcggcgc
     5101 cgccgtcgac gccaacgtca tcatcaacga gcgcatccgc gaggagcatc gccgcggccg
     5161 gcgggtgatg gaggcggtcg agcacggcta taacgaggcg cgcaccgcga tcttcgacgc
     5221 caacatcacc aacgtcatcg ccggcctgct gctctattat ttcggctccg gcccggtgaa
     5281 gggcttcgcc gtggtgatga tgatcggaat cgcgacctcg gtcttcaccg cggtcacggt
     5341 cacccggatg ttcgtctccc tctggctgcg caagcgcccc accgagctca ggatctgaag
     5401 gtcgccatgc gcctgctcaa actcatcccc gaggacacca atctcgactt cctgcgctgg
     5461 cgcaacgtcg ccatcgtcct ctcggcgctg ctgatggcgg cctcgatcgc cctgatcgcg
     5521 gtcaagggcc tcaacttcgg cgtcgacttc gccggcggcc aggtcatccg cacgaccttc
     5581 gcccagccgc cctcgctcga ccggctgcgc accgagatca ccggtctcgg cctcggcgat
     5641 ccgagcgtgc aggaattcgg gagcccgcgc gagatctcga tccgcctgcc cctcccgcaa
     5701 ggcggggagg aaggcgccaa cgccgccgcc acgaaggtgc gccaggcgct ccagcagcgc
     5761 tatcccgggg ttcggatcga cgcggtcgac accgtctccg gaaaggtgtc cgaggagctc
     5821 gtgtgggacg gcgctctggc gcttctgctc tccgccatcg gcatcgtcat ctacatctgg
     5881 atacgcttcg aatggcagtt cggggtcggc gcgctcttct cgctcttcca cgacgtcacg
     5941 ctcacgctcg gcttcttcgc gctgacccag atggagttcg acctcaacgt cgtcgcggcg
     6001 cttctgaccc tgatcggcta ttcgctcaac gacaccatcg tcatctacga ccgggtgcgg
     6061 gagaatttgc gcaaataccg caagatggac atcgtctcgc tcctcaacct gtcgatgaac
     6121 gagacgctgt cgcgcaccgt cgcgaccaac ttcgccatgc tgctcgcgct cggctcgctg
     6181 atgatccttg ggccgaacgt gatccagagc ttcaccaccg cgctcctgat cagcgtcatc
     6241 gtcggcacct actccaccgt ctacatcgcg gcgccctggc tggtctggct caaggtcgat
     6301 tcgaacagct tccttcccaa gaccaacgcc gcctccgccg gggagcgggt gacgagcctc
     6361 aacgaaggcg tctgagatgg cgcggctgga ccgcgatgcc ggcgcggacg ggcccgtcgt
     6421 caagggcttc gccaacggcg gcttcgtcgt cggcgagagc gtctaccgga cccttcagct
     6481 gactccgctt cgagccgagg aatgggccgc gccgccggtc gcgcagctcg ccgaggccga
     6541 cctcgacacc ctgctcgccc tcgatccggc cccggaattc ctgcttctgg gcaccggccc
     6601 gcgcctcagc cacccgccgc gcgcgttgcg cgaggcgctc gagcgccgcg gcatcggcct
     6661 cgaggtgatg gacagccgcg ccgccgcccg ggtctgggga ctgctgcgcg gcgaggagcg
     6721 ctggatcgtc gccgcgctga tgccgctcta gaacgagatg tgcttgcaga attcacccgt
     6781 cattgcgagc gaagcgaagc aatccagtcc tgcgcttttc gcgaaggccg agactggatt
     6841 gccgcgtcgc ttcgctcctt gcaatgacgg gtcgatctga ggcgaccacg ctctggaatt
     6901 atcggacgag ccccgccaga tgctgatgca gctcctccgc gttgcgctcc cagctgaacc
     6961 gttccgccgc ccggcgcacg gccgcgggat cgggcggtgc ggcgagcatc tcgagcgccg
     7021 ccgcggcgat cgcggccgga tcccgggcga ccagccggcc cgccgccggc cggtcgagca
     7081 cctcgcgcgc gccgccgacg tcggcgatca ggatcggggt gccgcaggcc agcgcctcga
     7141 cccagacgtt ggcgaggccc tcgcgctcgg aagggagcag catcaggtcg gccgcgccga
     7201 gcagctccgg catcgcctcc ggagcgacgc cgccgagcag ccggactcgg ctgcccagcc
     7261 cgagcttcgc gatcagcgcc tcgagcgcgg cccgctcggg cccctccccg gcgatcagca
     7321 gcgtcgctcc gggcagcagc gccgccgcct cgaccgccag ccgatggccc ttcagcggca
     7381 ccaaattgcc ggcggcgacg atcagcgggc cggccacgcc cagcgcgcgc ttcgcggccg
     7441 ggcggtcgag cggccggaag cggtcgagct cgaccccggt atggtgcacc cggatacggt
     7501 cctcgggcat ccccatcgcc gccatgtcga gccggagggc ggcgctcacc gccagcagcc
     7561 cgccggcccg cctcgccgcc tccagcatcc gcgcggcgat cgccggacgg cggccccaat
     7621 aatggatgtc gctgccgcgc gccttgatc
//
LOCUS       DASVQZ010000163         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_901371, whole genome shotgun sequence.
ACCESSION   DASVQZ010000163 DASVQZ010000000
VERSION     DASVQZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901371"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..>2891
                     /locus_tag="VF547_04645"
     CDS             299..>2891
                     /locus_tag="VF547_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262142.1"
                     /translation="MAARERKGGARHSGAKRRKAASGASRHDDACPAIKNALLRLAAT
                     KDGFEGLVAALVEAATGGPLLLMSSGDQGGLDAIGADRGSAPRRAMQAKRYGDTSKLN
                     LNTLVGEMSRAASAFPHIDCWIVPTTQPLWGKEKEALRRHAQSLGWGFVALDWSPGLN
                     LTPRLALLCAAYPEVTARWDELASIRSELDAIAADAGFEAAAAALIGELVAADVGFRS
                     AQAAALAHLERAYRDPAAARHIAGASPAFLAKAPPVPREGPRKQILDWYCGTQKALVL
                     LGGEGAGKTWAALDGLRAAAETMPYPLPVVIGAARARRHPNGLAAVASALADVGTFAE
                     LSLDDPMDFWTRRLARWARTGPGADPVILMLVDGLDEVDSFDWQDWLAPLFTAENSAL
                     FRVLLTCRADDWQQGLSRSLADAGNDEVAVATVGNFEPHERDSYLAEHGIEDLEKVAP
                     NVLAAALHPRTAFHLIRHAREIGGLTQITREQLLLRDFRNRREIKGGRLTEHAFAELV
                     CGLAVQAQNAALRQQAFRVSEGEVVEQAADIAGFGRREMRWVLSELVSGHWCTREPAR
                     PNQITFTNDALPDAVGMALAAQVSGLDAESALAEIDRFLEPWGADDLVEPVLRMCATA
                     LVVDPAVADALCEAVLERWQRRSFHGSAGQDFWRRLHVFRPNVFLTLAERRAHRESGW
                     LMEWGLACFWEDYPEYRPLVAERLGAWISVLPLPRFDDSAERPYRRYANRDRTRQLRR
                     LAALERRRLPGWQRRIGQEADEAEAQRIHTAMRVIGFLPRAPFVPLLAEWALTMAAAG
                     RVVRERDVAALLRDNDLDHDGALEAVHWEVARLERGGTALERRAAALLLRATGRGEDH
                     ERAQRLSP"
BASE COUNT          441 a          998 c         1013 g          439 t
ORIGIN      
        1 cggcgacgat ccggtcggcc ttctcgagct tgggcgcctc gggcgcggcc gcctcggcga
       61 cccgctcgag caggaccggg tccggctgcg gctcgggcac ggcctcgccg caggcgccga
      121 gcgccgccag gaccacgccg gcaaaggcaa atctttccat catcttgccc tgccaacgcg
      181 cgggcgcgcc ggaaggatgc agggagcggg cggctttccc tcggcggcag gctgaccttg
      241 ataggcggcc ttgctaaggc ggaggcgcta cgggcgagac accgcgaagg gtgaaggcat
      301 ggcggcacgc gaaaggaagg gtggcgcccg tcattccggg gcaaagagaa gaaaggcggc
      361 gtccggcgct tctcgccacg atgacgcctg ccccgcgatc aagaacgcgc tgcttcggct
      421 cgccgccacg aaagacggat tcgagggcct cgtggccgcc ctggtcgagg cggcaaccgg
      481 cgggccgttg ttgctgatgt cgtcgggcga tcagggcggg ctcgacgcaa tcggcgccga
      541 ccgcggctcc gccccgcggc gcgccatgca ggcgaagcgc tacggcgaca cctcgaagct
      601 caacctgaac acattggtcg gcgagatgag ccgcgccgcc tcggcttttc cgcatatcga
      661 ctgctggatc gtccccacga cccagcccct ttggggaaag gaaaaggagg cgcttcgccg
      721 ccacgcccaa tccctcggct ggggcttcgt cgctctcgac tggtcgcccg gactgaacct
      781 gacccctcga ctggcgctgc tctgcgctgc ctatccggag gtgacagcca ggtgggacga
      841 gcttgcgtcg atcaggagcg agctcgacgc cattgccgcc gatgcgggat tcgaggccgc
      901 ggcggcggcc ctcatcggcg aactcgtcgc cgcggacgtc gggttcaggt ccgcccaggc
      961 cgccgccctg gcgcatctcg agcgagccta tcgcgacccg gcggcggccc ggcacattgc
     1021 cggcgcctcc cctgccttcc ttgccaaggc accgccggtg ccgcgggagg gaccgcgcaa
     1081 gcagatcctg gactggtatt gcggaaccca aaaggccctc gtgcttctcg gcggcgaggg
     1141 ggccggcaag acctgggcgg cgctggacgg gcttcgagcg gcggccgaga cgatgcccta
     1201 tccactgccg gtcgtcatcg gtgcggcgcg cgcgcggcgg catcccaacg gcctcgccgc
     1261 ggtcgcgtcc gcattggccg acgtcggcac cttcgccgaa ttgagcctcg acgatcccat
     1321 ggacttctgg acccgccggc tcgcgcgatg ggcgcgaacc gggccgggtg ccgatccggt
     1381 gatcctcatg ctggtcgacg gcctcgacga ggtggattcg ttcgactggc aggactggct
     1441 ggcgccgctg ttcactgccg aaaactcggc tttgttccga gtgttgctga catgccgggc
     1501 ggacgattgg caacaagggc tcagccgcag cctcgccgat gcggggaacg acgaggtggc
     1561 ggtcgcgacc gtcggcaatt tcgagcccca cgaacgcgat tcctatctcg ccgaacacgg
     1621 gatcgaagac ctggagaagg tcgcgcccaa cgtcctcgca gcggccctgc atccgcggac
     1681 ggccttccat ctcatccgtc acgcgcggga gattggcggc ctgacgcaga tcacgcgcga
     1741 acagcttctc cttcgggatt tccgcaatcg ccgcgaaata aagggcggcc ggctcaccga
     1801 gcatgccttt gccgaactgg tctgcggcct ggctgtccag gcgcagaacg ccgcgcttcg
     1861 ccagcaggcg tttcgagtca gcgaaggcga ggtcgtcgaa caggccgccg acatcgccgg
     1921 tttcggccga cgcgagatgc ggtgggtgct gagcgagctt gtctcagggc actggtgcac
     1981 gcgcgagccg gctcgaccaa atcagatcac cttcaccaat gacgctttgc ccgacgccgt
     2041 cgggatggct ctcgcggcgc aggtgagcgg gctcgatgcg gaatcggcgc tcgcggagat
     2101 cgaccgattc ctggagccat ggggcgcaga cgacctggtc gagccggtgc tgcgcatgtg
     2161 cgccacagca ctcgtcgtcg atcccgccgt cgccgatgcc ctgtgcgaag cggtgctcga
     2221 acgctggcag cgacggtcgt tccacggcag tgccgggcag gatttctggc gccgcctgca
     2281 tgtgttccgg cccaacgtct tcctcaccct ggccgagcga cgcgcccatc gggagagcgg
     2341 atggctgatg gaatgggggc tcgcctgctt ctgggaggac tatcccgaat atcgaccctt
     2401 ggttgccgag cgtctcggcg cctggatttc ggtgctgccc ctgcctcgat tcgacgatag
     2461 cgccgagcgg ccctatcggc ggtacgccaa ccgcgaccgc acccgccagc tccgtcgcct
     2521 cgcggcgctg gagcggcgac gccttcccgg ctggcaacgg agaatcgggc aggaggccga
     2581 cgaggccgaa gcgcagcgca tccataccgc gatgcgggtg atcggcttcc ttcccagggc
     2641 cccgttcgtt ccgctgctgg cggagtgggc cctcaccatg gccgcggccg gccgagtcgt
     2701 tcgcgagcgc gacgtggcgg cgctactcag agacaacgac ctcgatcatg acggcgccct
     2761 ggaagcggtc cactgggaag tggcgaggct cgagcgcggc gggacggcgc tcgagcgccg
     2821 cgccgccgct ctcctgctga gggcgacagg ccgcggcgag gaccacgagc gggcgcaacg
     2881 gctcagccct c
//
LOCUS       DASVQZ010000164         5418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_514092, whole genome shotgun sequence.
ACCESSION   DASVQZ010000164 DASVQZ010000000
VERSION     DASVQZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514092"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VF547_04650"
     CDS             <1..695
                     /locus_tag="VF547_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX8262143.1"
                     /translation="ASCHGLSFVAFRDLHDLGYGEEEVKAIAEQWQIEVPAVDAKTGE
                     ASTRKALPADRFPDPYPNETAARAANRHALPPDLSLITKARHGGPAYVASLLTGYRNP
                     PASLPAENRPGEGLHFNPWFANLNIAMPPPLTGDGQVSYADGTNPTREQMATDVSAFL
                     AWTAEPKMENRKAAGLATLIFLIFATVLAYLAYQNIWHGGASRRVRETGPLDPDNMAK
                     RDEASREAGIEG"
     gene            1023..2462
                     /locus_tag="VF547_04655"
     CDS             1023..2462
                     /locus_tag="VF547_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262144.1"
                     /translation="MAKQKKIRVPQFSEKLLWTIEHLVSQRPALASSGEQITPRQPAE
                     IRSLSALYWRMENDAADAWFPKRENWLEDVRNDKCSPEILAMICDVYPELTPDLIRDT
                     DFEGFQAACAPLVSARDHYIGLGRHFLAERERLAAFAKDYYSDEDELPDWPLLGRRGW
                     LPELPIPISESVSIDRFEPSSSYPDARCLPGLNARYGQIRRLLSPTAKPPFNGDCYRL
                     LSMQLENSGAPIFTYGPCKYFDYYDTCEVHALKIASLERDGKAVVDIDPFDIAGHAAV
                     PGVNTLTVFLNFPRFGQNRFLLHRRSGATVQAGNTLHVVPSGQHQPSQAFYGLDADVS
                     IWRTMVREFCEELFNVAEAHGLGIDAGDPLDAPDCRRIVEPLFRSRASRAYLLGMGLD
                     PVTAKPEILVVNIIDFRKLTQQQRDAFSRQKANWEGQLHYFDLTETQIRTQLELDRSH
                     DLKWLPAGKACLTEFLRHFRWLMEDSQRH"
     gene            2591..3271
                     /locus_tag="VF547_04660"
     CDS             2591..3271
                     /locus_tag="VF547_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262145.1"
                     /translation="MSLVRLRSAALVLAAGLGLSACSTYDGYGYGGLSVGYGSYCDPY
                     YDDCYAGYGGYGGYYDPWYGWYGDYYYPGIGIYVFDRWGRRHRWNDDHRRYWEGRRGR
                     WGDRDWNDRRWERWDGYRGRRGGDWDRDRRDRDPRWRGDRSDRQDWREDRQQIRENFR
                     EFRQDRRGDRTQFRTERQQDREALQRGDVTREQFRQDRRQDRREFRREGRQDWRELRR
                     ENRRDRRD"
     gene            complement(3272..3724)
                     /locus_tag="VF547_04665"
     CDS             complement(3272..3724)
                     /locus_tag="VF547_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8262146.1"
                     /translation="MIYFEDLEIGRETEFGSYEVTRAEVLDFARKYDPQPFHLSDEAA
                     AKTHFGRLAASGWHTCAMTMAVIARFVVEDEQAGLGSPGVDELRWLKPVYPGDTLTVR
                     STIIEKTPSRSKPDLGSFRSATVVTNQDGVPVLTFTSIVLIRRRPAQP"
     gene            4017..4271
                     /locus_tag="VF547_04670"
     CDS             4017..4271
                     /locus_tag="VF547_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262147.1"
                     /translation="MLTTIEGGRSYPHDPALRGFQILYHSGEINRCPGCGRTHWYVGR
                     LSAECGFCGTALALAETARTGTGVFHRGQPARDDSWDIAA"
     gene            complement(4294..4956)
                     /locus_tag="VF547_04675"
     CDS             complement(4294..4956)
                     /locus_tag="VF547_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8262148.1"
                     /translation="MLTLHYHPFSSYCQKVLVALYERGVPFERNIVNLGDPAEKAALE
                     RLWPMAKFPVLRDEVAGLTVPESSLIIAYLDRVHPGPPLVPADPDAALHAGLWDRFLD
                     HYVETPLQKAVGDHFRPETARDPHGVEEAKALLGRAWDMLEERLADGRPWLTGDEFTI
                     ADCGAGPALFYGNIVEPFAGRPQLEAYYARLLARPSFARAVEEARPYRPFFPLPWPES
                     YA"
     gene            5072..>5418
                     /locus_tag="VF547_04680"
     CDS             5072..>5418
                     /locus_tag="VF547_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262149.1"
                     /translation="MRILLLAALALVPAAAPAQAPATVAAESPLVAQARAFMKSYGED
                     LVQGNRAAVAARYDPTGAWHVGPTGAELESWDKIDSRYRRRWSPPSSFEWQGLAFEQV
                     GPDTVLVVGGFHW"
BASE COUNT          908 a         1857 c         1763 g          890 t
ORIGIN      
        1 gcgcctcctg ccacggcctc agcttcgtcg ccttccgcga cctccacgat ctcggctatg
       61 gcgaggagga ggtgaaggcg atcgccgagc agtggcagat cgaggtcccc gcggtcgacg
      121 cgaagaccgg cgaggcgagc acccgcaagg ccctgcccgc cgaccgcttc ccggatccct
      181 atccgaacga gacggcggcg cgcgccgcca accgccacgc cctgccgccc gacctgtcgc
      241 tgatcaccaa ggcgcgccac ggcggtccgg cctatgtcgc ctccctgctc accggctacc
      301 gcaacccgcc ggccagcctg cccgcggaga accggccggg cgaggggctc cacttcaatc
      361 cctggttcgc caacctcaac atcgccatgc cgccgccgct caccggcgac ggccaggtca
      421 gctatgccga cggcaccaac ccgacccgcg agcagatggc gaccgacgtc tccgccttcc
      481 tcgcctggac cgccgagccg aagatggaga accgcaaggc ggcgggcctc gccaccctga
      541 tcttcctgat cttcgcaacg gtcctggcct atctggccta tcagaacatc tggcacggcg
      601 gcgccagccg ccgggtgcgc gagaccggcc cgctcgaccc ggacaacatg gccaagcgcg
      661 acgaggcgag ccgcgaggcg ggcatcgagg gctagaatcg ggctaggcgg cgatccggtc
      721 cggatcggtc gaaagcatta cagaaggtta caaacgcggc gtccgacagt cgcctaaatc
      781 gattccggcc aatggttggc ccggagtcga gacatgctgt tcgaatcaaa aatcgtcgga
      841 tatttccgca ggaaaaccgc cgcctcaaag cctcgggtgc tcccgcgggg cgcccgccgc
      901 gcgcaggcag ggccggtgcc cgggcgaggg tcgccttccc atgcagccga agccttgcgg
      961 ggaggcattc ggatcatcct ttggcttctc gagttgatcg gcaacctcga ctgaggcgcg
     1021 tcatggcgaa gcaaaagaaa atcagggttc cgcaattcag cgagaagctg ttgtggacca
     1081 tcgagcatct cgtctcccag cgtcctgcgc ttgcgagctc gggggagcaa ataacgcctc
     1141 ggcaacccgc ggaaattagg agcctgtctg cgctgtactg gcgtatggag aacgatgcgg
     1201 ccgatgcctg gtttccgaag cgggagaatt ggctcgaaga cgtcaggaac gacaagtgct
     1261 cgccagaaat cctcgcgatg atctgcgatg tctatcccga gctcacgcct gacctgatta
     1321 gagacacgga tttcgagggt tttcaggcgg cttgtgctcc cctcgtgagt gcccgcgacc
     1381 attatatcgg gctggggcgc catttcctgg cagagcggga aaggctcgct gcctttgcga
     1441 aggattatta ttcggatgag gacgaactcc ccgactggcc gctgctgggc cggcgcggtt
     1501 ggctgccgga gctgccgatt ccgatcagcg agtccgtttc gatcgaccgt ttcgagcctt
     1561 catcgagcta ccccgatgcc cgctgcctgc ctggcctcaa cgcccgctac gggcaaatac
     1621 gcaggctgct ttcgcctacc gcgaagccgc cattcaatgg cgattgctat cgcctgctct
     1681 cgatgcagct ggagaatagc ggcgcgccga tcttcaccta tggtccgtgc aagtatttcg
     1741 attattacga tacctgcgag gtccacgcgc tcaagatcgc ctcattggaa cgggacggga
     1801 aagcggtggt cgacatcgac cccttcgaca tcgccggcca tgcagcggtg cccggagtaa
     1861 acacccttac cgttttcctc aacttcccga gatttggtca gaaccgtttc ctgctgcacc
     1921 gtcgttccgg ggcgaccgtc caggccggca acacgctgca cgtcgtacct tcgggtcagc
     1981 accagccgtc gcaggctttc tatggcctcg acgccgacgt ctcgatctgg cggacgatgg
     2041 ttcgcgaatt ctgcgaggaa ttgttcaacg tagccgaggc gcacgggctc gggattgacg
     2101 ccggcgatcc cctcgacgct ccggattgcc ggcgtatcgt cgaacctctc ttcaggtcac
     2161 gggcgagccg ggcctatctg cttgggatgg gcctcgaccc ggtcacggcg aaacctgaga
     2221 tccttgtcgt caacatcatc gactttcgta agctgacgca gcagcagcgc gatgctttca
     2281 gccgccagaa ggccaattgg gaagggcagc tccattattt cgatctgacc gaaacccaaa
     2341 tccgcaccca acttgaactc gaccgcagcc acgatctgaa atggctcccc gccgggaaag
     2401 cctgcctgac ggagttcttg cgccatttcc gttggttgat ggaagattcg caacgtcatt
     2461 gacgactgct tcagacctga cgggttttgc aggaacctcc tcctttacag ggggttaacg
     2521 ttcagcggcg gttcacgccg cgaggcggat tttccccgcc gcctaagcgt ctttttcggg
     2581 agagtcgctc atgtccctcg tccgcctgcg ctccgccgcc ctggtcctcg ccgccggcct
     2641 cggcctttcg gcctgctcga cttacgacgg ctacggctac ggcggcctgt cggtgggcta
     2701 cggctcctat tgcgacccct attacgacga ttgctacgcc ggctacggcg gatacggcgg
     2761 ctattacgat ccgtggtacg gctggtacgg cgactattat tatcccggca tcggcatcta
     2821 cgtgttcgac cgctggggcc gccgtcaccg ctggaacgac gaccaccgcc gctactggga
     2881 aggccgccgc ggccgctggg gcgaccgcga ctggaacgac cgccgctggg agcgctggga
     2941 cggttatcgc ggccgccgcg gcggcgactg ggaccgcgac cgccgcgacc gcgacccgcg
     3001 ctggcgcggc gaccgcagcg accgccagga ctggcgcgag gaccggcagc agatccgcga
     3061 gaatttccgc gagttccggc aggaccgccg cggcgatcgc acccagttcc gaaccgaacg
     3121 ccagcaggac cgcgaggcgc ttcagcgcgg cgacgtgacc cgcgagcaat tccgccagga
     3181 ccgccgccag gatcgccgcg agttccgccg cgagggccgc caggactggc gcgagctccg
     3241 gcgcgagaac cggcgggacc ggcgcgactg actaaggctg ggccggccgg cgcctgatca
     3301 ggacgatcga ggtgaaggtc aggaccggaa cgccgtcctg gttggtgacc accgtcgccg
     3361 agcggaacga gccgaggtcc ggcttgcttc gcgacggcgt cttctcgatg atggtgcttc
     3421 gcacggtgag cgtgtcgccc ggatagaccg gcttcagcca gcgcagctcg tcgacgccgg
     3481 gggagccgag cccggcctgc tcgtcctcga cgacgaagcg cgcgatcacc gccatcgtca
     3541 tcgcgcaggt gtgccagccg ctcgccgcga gccgcccgaa atgggtcttc gccgcggcct
     3601 cgtcggaaag gtggaagggc tgggggtcgt atttgcgggc gaagtccagc acctccgcgc
     3661 gggtcacctc atagcttccg aactccgtct cgcggccgat ctccaaatcc tcgaaataga
     3721 tcatcggcct gactcccgtc gcgccggctc cgccggcctt ccacctttgc ttcccgacgc
     3781 gcattgagga aggcgggccc cggatcaaga cccggcgtga cggtcccgcc acgccccgcg
     3841 gcccgaaaat gccctttttc cgcccggaac ggcgccctct gtcaccgccc ggtggcagcg
     3901 gaacaacaaa atggtctcga ttgtgtcacc cccgcaacat taatcttgcg ttcaacccgt
     3961 ctatggaaaa caacggctcg tcgcagccgt cgctgcgacg agtcgaggaa aaagagatgt
     4021 tgacgacgat cgaaggcggc cggagctacc cccacgaccc ggccctgcgc gggttccaga
     4081 tcctgtatca ttcgggcgag atcaaccgct gcccgggctg cggccgcacg cattggtacg
     4141 tcggacggct ctcggccgag tgcggcttct gcggcacggc cctcgctctg gcggagacgg
     4201 cgcgcaccgg caccggcgtc ttccacaggg gccagcccgc gcgcgacgat tcctgggaca
     4261 tcgccgcctg agccgccggc cgggcgaggc gcgtcaggcg tagctctccg gccagggcaa
     4321 ggggaagaag gggcggtacg gccgcgcttc ctcgaccgcc ctggcgaagg acggccgggc
     4381 gaggagccgg gcgtaatagg cttccagctg cggccgcccc gcgaagggct cgacgatatt
     4441 gccgtagaag agggccgggc cggccccgca atcggcgatc gtgaactcgt ctcccgtcag
     4501 ccagggccgt ccgtcggcca gccgttcctc gagcatgtcc caggcgcgcc caagcagcgc
     4561 cttcgcctcc tcgacgccgt gcgggtcgcg ggcggtttcc ggacggaaat ggtcgccgac
     4621 ggccttttgc agcggagtct cgacataatg gtcgaggaac cggtcccaca gaccggcgtg
     4681 aagcgccgcg tcgggatccg cggggacgag cggcggcccg ggatggacgc ggtcgagata
     4741 ggcgatgatc aggctcgatt ccggcacggt caggccggcc acttcgtcgc gcagcaccgg
     4801 gaacttggcc atcggccaca gccgctcgag cgccgctttc tcggccgggt cgccgaggtt
     4861 gacgatgttg cgctcgaacg gcacgccgcg ctcgtagagc gcgaccagca ccttctggca
     4921 gtaggaggag aaggggtggt agtggagggt gagcatgcgg agggcttagc ggccgcggag
     4981 ggggccgtaa attgctccct gacggcgggc cgggagaggc gaatcgccga gcgcattgca
     5041 tcggcggccc gctggtgaca ttatccccct catgcgcatc ctcctcctag ccgccctcgc
     5101 tctggtgccg gccgccgcgc ccgcccaggc tcccgccacc gtcgccgccg agtcgccgct
     5161 cgtcgcccag gcccgcgcct tcatgaagag ctatggcgag gatctcgtgc aggggaaccg
     5221 cgccgccgtc gccgcgcgct acgatccgac cggcgcctgg cacgtcgggc cgaccggagc
     5281 ggagctcgag agctgggaca agatcgattc ccggtaccgc cgccgctggt cgccgccctc
     5341 gagcttcgag tggcagggcc tcgccttcga gcaggtcgga ccggacacgg tgctggtcgt
     5401 cggcggcttc cactggtg
//
LOCUS       DASVQZ010000165         5931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_420228, whole genome shotgun sequence.
ACCESSION   DASVQZ010000165 DASVQZ010000000
VERSION     DASVQZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420228"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF547_04685"
     CDS             <1..207
                     /locus_tag="VF547_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262150.1"
                     /translation="IPPELRDDTSDDPESTSWAARAEALEYVGRTGIQSCSTVGPGGA
                     SGCWNELVRAWRQERRQGDGEPGR"
     gene            complement(326..2074)
                     /locus_tag="VF547_04690"
     CDS             complement(326..2074)
                     /locus_tag="VF547_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8262151.1"
                     /translation="MRIWLLAGAALLAAAPATAAPADDFKRLADDYYAWLLRENPTYA
                     TALGVRDHDDRIEDLSLAARDRQAAEAKALLARLDRIGEGGLSPADRVNRAILKRALE
                     EGIAANGFRQRVMLFTTYAGWHQNFAGLADNLPFRTKADYESYLVRIAQYPRQNDAAL
                     DITAQAVRGGFVLPCSVLGNYEKGIAGVIADDPAKSRFYEPFTRARPSDVTEAEWTAM
                     QARARKLVSDVLNPAYAKHLDFYRTTYAPKCAKVDSISAQPGGREYYAFRVRQETTTD
                     LTPEQIHDIGLRESARIRAEMEQVAKSAGFPGREAFIRELRTNPKYFTRTPTELMEAA
                     ALTAKTIDGKMPGWFATLPRLPYGVRPIPAEIAEGTTTAYYGQGSLASGLSGTYWVNT
                     SKLDQRPLWELPALTLHEAVPGHHHQISLQQEVDLPPFRRHFTFFTAFTEGWALYGES
                     LGEEMGLYDTPEKKMGQLSYQAWRAARLVVDTGIHSKCWDKAKAVAFMRDNTALTDAN
                     IDAEVNRYISWPGQALGYKIGELRIRELRARAERELGTKFDLRRFHDAVLLQGSVPLD
                     VLDAQVGAWIEAEKGR"
     gene            2278..2487
                     /locus_tag="VF547_04695"
     CDS             2278..2487
                     /locus_tag="VF547_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEX8262152.1"
                     /translation="MSDNVYKVIEIVGSSPTSIEDAIEGAIRRAAATLRDIGWFEVTE
                     TRGHVENGRVAHYQVTLKIGFTLRD"
     gene            2546..3061
                     /locus_tag="VF547_04700"
     CDS             2546..3061
                     /locus_tag="VF547_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262153.1"
                     /translation="MATTAAAAPPRARISLEPETFDRILSGAAMVLLGFVFTALWRGR
                     PEWGEVPAFVWGHISTIVLATLLTPVMLLRRRGDRLHRRLGWVWVTSMALTAVLTFWI
                     RGINQGSLSLIHVLSAWTLVQVPLIVWTARNHHHRRHRNSVRGMVSGALIIAGIFTFP
                     FDRLLGRWLFG"
     gene            3194..4594
                     /locus_tag="VF547_04705"
     CDS             3194..4594
                     /locus_tag="VF547_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX8262154.1"
                     /translation="MTAAEATPELDIAADRDDRAFLGHPKGLAYLAFTEAWERFSYYG
                     MQALLVLYLVNYLLKPEHVGSVAFFDSFRTLYGGLDGQPLASAIVGTYFASVYLTPIL
                     GGFLADRVLGKRRTILLGAVTMALGHFLMAFEASFLFALLCLILGSGMFKGNIASQVG
                     SLYKAEDLRRADAFQIFYLGINAGVIAAPLIVGTLGEKYGWHWGFGAAGVGMLISLVI
                     YLAGQKYLPREHFDVPQRSARAAAPRLDRKDWLATLALILLIPVMAVALVPNNQIFNA
                     YLVWGDQQFNLVFRGEKLPTTWLVTLDAIVSVSFLAGVALFYRWYGKRRREPDELTKI
                     IIGSAFSIAGMLCLFLAAWTQGPGDKIGLFWPVMFHVLNSIGFAHMLPVSLALFAKLA
                     PKAINAIVIGLYYLAFFAGNSMVGWIGGFLSTMPATDFWLLHAGLAAGSGAVFILFKL
                     FAGRHFDDREPADALT"
     gene            4874..>5931
                     /locus_tag="VF547_04710"
     CDS             4874..>5931
                     /locus_tag="VF547_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEX8262155.1"
                     /translation="MAQLDLQQKLAVLADAAKYDASCASSGTAKRDSRGGKGIGSTEG
                     MGICHAYAPDGRCISLLKILLTNSCIFDCHYCINRKSSNVRRARFTAEEVVRLTLAFY
                     KRNYIEGLFLSSGIIRSSDYTMEQLVEVARSLREDHDFRGYIHLKTIPDADPELVRLA
                     GVHADRVSINVELPTAAGLARLAPEKDGDRIEGAMKDMRSAIEEGADAGKRFRSAPAF
                     APAGQSTQMIVGADSATDADIVGRASALYDRFRLRRVYYSAFSPIPDASSVLPLRRPP
                     LMREHRLYQSDWLMRFYGFAPGEVAEAAGDDGMLPLDIDPKLAWALKFRESFPVDVNR
                     APREALLRVPGLGVKAVDR"
BASE COUNT          866 a         2145 c         1902 g         1018 t
ORIGIN      
        1 atcccgcccg agcttcgcga cgacacgtcc gacgatcccg agagcaccag ctgggcggcc
       61 cgcgccgaag cgctcgaata tgtcggccgc accggcatcc agagctgctc gacggtcggc
      121 cccggcggcg ccagcggctg ctggaacgag ctggtccgcg cctggcggca ggagcgccgc
      181 cagggggatg gagagccggg gcgctagggt ccttcgatac gccggttcga caagctcacc
      241 ggctactcag gatgagcggt agagagaaac cccaaccgct catcctgagt agggactgag
      301 cgtggcgaag gcccgtatcg aaggactacc tccccttctc cgcctcgatc cacgccccga
      361 cctgggcatc gagcacgtcc agcggcacgc tgccctggag cagcaccgcg tcgtggaagc
      421 gcctcaggtc gaacttcgtc cccagctcgc gctccgcccg cgccctcagc tcgcggatcc
      481 tcagctcgcc gatcttgtag ccgagcgcct ggcccggcca gctgatgtag cggttgacct
      541 cggcgtcgat attggcgtcg gtcagcgccg tattgtcgcg catgaaggcc accgccttcg
      601 ccttgtccca gcacttggaa tggatgccgg tgtcgaccac cagccgagcc gcccgccacg
      661 cctggtagga gagctgcccc atcttcttct cgggcgtgtc gtacaggccc atctcctcgc
      721 ccaggctctc gccgtagagg gcccagcctt cggtgaacgc ggtgaagaag gtgaaatggc
      781 gccggaaggg cggcaggtcg acctcctgct ggagcgagat ctgatggtga tggccgggga
      841 cggcctcgtg cagggtcagc gccggcagct cccagagcgg cctctggtcg agcttcgagg
      901 tgttgaccca gtaggtgccg gacagcccgc tggcgagcga gccctggccg taataggcgg
      961 tcgtcgtccc ctccgcgatc tccgccggta tcgggcgcac gccatagggc aggcgcggca
     1021 gcgtcgcgaa ccagccgggc atcttgccgt cgatcgtctt ggcggtgagc gccgccgcct
     1081 ccatcagctc cgtcggcgtg cgggtgaaat atttggggtt ggtccgaagc tcgcggatga
     1141 aggcttctcg gcccggaaag ccggccgact tcgcgacctg ctccatctcg gcccggatcc
     1201 gcgccgattc ccgcaggccg atgtcgtgga tctgctcggg cgtgaggtcg gtcgtcgtct
     1261 cctgccgcac ccggaaggca taatattcgc ggccgccggg ctgcgccgag atgctgtcga
     1321 ccttggcgca tttgggcgca taggtcgtgc ggtagaagtc gagatgcttc gcataggccg
     1381 ggttcagcac gtcgctgacg agcttgcggg cccgggcctg catcgccgtc cactcggctt
     1441 cggtgacgtc ggacggccgg gcccgggtga acggctcgta gaagcgcgac ttggcagggt
     1501 cgtcggcgat cacccccgct atgcccttct cgtaattgcc gagcaccgag cagggcagga
     1561 cgaagccgcc cctgaccgcc tgggcggtga tgtcgagcgc ggcgtcgttc tgccggggat
     1621 attgcgcgat ccggaccaga tagctttcgt aatccgcctt ggtccggaag ggcagattgt
     1681 cggccagccc ggcgaagttc tggtgccagc cggcataggt ggtgaagagc atcacccgct
     1741 gccggaagcc gttggccgcg atcccctcct ccagcgcgcg cttcaggatc gcccggttga
     1801 cccggtcggc cggcgacagg ccgccctcgc cgatccggtc gagccgcgcc agcagggcct
     1861 tggcctcggc cgcctggcgg tcccgggcgg cgaggctgag gtcctcgatc ctatcgtcat
     1921 ggtcgcgcac tccgagcgcg gtggcgtagg tcgggttctc gcgcagcagc caggcgtaat
     1981 aatcgtcggc cagccgcttg aaatcgtccg ccggcgccgc agtcgcgggc gccgccgcga
     2041 gcagcgccgc gcccgccaga agccaaatcc tcatcgcttt ctcctcgcct tgcagcgtcg
     2101 cgccgatcgt cccgggtttc cgttcgggct gagcctgtcg aagccctata cttcccggtg
     2161 caggggcgga aggaaagccc ttcgacgggc tcggggcgaa cgaggtcggc ccccttccgg
     2221 gccgccgccc cgcaacgatc gcgtccaccg cccgttggac gggaacggga ggtcccaatg
     2281 agcgacaatg tctacaaggt gatcgagatc gtcggcagct cgccgaccag catcgaggac
     2341 gcgatcgagg gcgcgatccg ccgcgccgcc gctaccctcc gagacattgg ctggttcgag
     2401 gtcaccgaga cccgcggcca tgtcgagaac ggcagggtcg cccattacca ggtgaccttg
     2461 aagatcggct tcaccctccg cgattgagcc gccgggcttg cgcggcccgg ccaagcgaag
     2521 catcttcgcg ccggggaggg acgccatggc aacgaccgcc gccgctgcgc cgccgcgcgc
     2581 caggatcagc ctcgagccgg agactttcga ccgcatcctg tcgggtgccg cgatggtgct
     2641 gctcggcttc gtcttcaccg ccttgtggcg cggccgcccg gaatggggcg aggtccccgc
     2701 cttcgtctgg ggccatatct ccaccatcgt cctcgccacg ctgctgaccc cggtgatgct
     2761 gctgaggcgc cgcggcgacc ggctgcaccg gcgcctgggc tgggtctggg tgacgtccat
     2821 ggccctcacg gcggtcctga ccttctggat ccggggcatc aaccagggct cgctcagcct
     2881 catccacgtc ctctccgcct ggaccctcgt ccaggtgccg ctgatcgtct ggacggcccg
     2941 caaccaccac caccgccgcc accgcaactc ggttcgcgga atggtgtcgg gggcgctcat
     3001 catcgccggg atcttcacct tcccgttcga ccggctgctc ggccgctggc tgttcggctg
     3061 aggccgccgc cgggagcgaa gcggcgctgc gcgcgtcgcg gtggcgggca cgccagggct
     3121 ttccgtcgaa aaatgctggc gcgcccgtaa cgatccacta gcgtccgcgc gatcttaaaa
     3181 gggggagcgc agcatgaccg ccgccgaagc cacgcccgag ctcgacatcg ccgccgatcg
     3241 ggacgaccgc gccttcctcg gccatcccaa ggggctcgcc tatctcgcct tcaccgaggc
     3301 gtgggagcgc ttctcctatt acggcatgca ggcgctgctc gtcctctacc tcgtcaacta
     3361 cctgctcaag ccggagcatg tcggcagcgt cgccttcttc gacagcttcc gcaccctcta
     3421 cggcgggctc gacggccagc cgctcgcctc ggccatcgtc ggcacctatt tcgcgtcggt
     3481 ctacctcacc ccgatcctcg gcggcttcct cgccgaccgg gtgctcggca agcgccgcac
     3541 catcctgctc ggcgccgtca ccatggccct cggccatttc ctgatggcgt tcgaggcgag
     3601 ctttctgttc gccctgctct gcctcatcct cggcagcgga atgttcaagg gcaatatcgc
     3661 gagccaggtc ggatcgctct acaaggccga ggacctgcgg cgcgcggacg ccttccagat
     3721 cttctacctc ggcatcaacg ccggagtcat cgccgctccg ctgatcgtcg gcacgctcgg
     3781 cgagaaatac ggctggcact ggggcttcgg cgcggccgga gtggggatgc tgatctcgct
     3841 cgtcatctac ctcgccggcc agaaatatct tcccagggag catttcgacg ttccgcagcg
     3901 ctcggcgcgc gccgccgccc ccaggctcga ccgcaaggac tggctggcga ccctggccct
     3961 catcctgctg atcccggtga tggcggtggc cctggtgccg aacaaccaga tcttcaacgc
     4021 ctatctcgtc tggggggacc agcagttcaa cctcgtcttc cgcggcgaga agctgccgac
     4081 cacctggctg gtgaccctcg acgccatcgt cagcgtcagc ttcctcgccg gcgtcgccct
     4141 cttctaccgc tggtacggca agcggcgccg cgagcccgac gagctcacca agatcatcat
     4201 cggctcggcc ttctcgatcg cgggcatgct ctgcctgttc ctcgccgcct ggacgcaggg
     4261 accgggcgac aagatcggcc tgttctggcc ggtcatgttc cacgtcctca actcgatcgg
     4321 cttcgcgcac atgctgccgg tcagcctcgc cttgttcgcc aagctcgcgc ccaaggcgat
     4381 caacgcgatc gtgatcggcc tctactatct cgccttcttc gccgggaact cgatggtcgg
     4441 ctggatcggc ggcttcctct cgaccatgcc cgccaccgac ttctggctcc tccacgccgg
     4501 cctcgccgcg ggctcgggcg ccgtcttcat cctgttcaag ctgttcgccg gccgccactt
     4561 cgacgaccgc gagccggcgg atgcgctgac ctgattcatc gcccgccggg caagccccgg
     4621 gccccgaaac tgcccgatcc gctcgaagga acaaaaagaa aacccttccc taggtcgctg
     4681 atttaggcga taaaatcggg atggtcgaat cgcttcgctt gcctctgcga cgttccttgg
     4741 agtcgcctcc tgcggacggc ctgcctgatc cggccgaagc ttgcatcggg cgggccggaa
     4801 aggattcttc aggccgactt gcgggtcttt tgtgtctttt gggcccctat ccgtcggccg
     4861 cggggccggt ttgatggccc agctcgacct ccagcagaag ctcgccgtcc tcgccgatgc
     4921 ggccaagtac gacgcttcct gcgcctcctc cggaaccgcg aagcgcgaca gccgcggcgg
     4981 caaggggatc ggctcgaccg aggggatggg catctgccac gcttacgccc ccgacggccg
     5041 ctgcatctcg ctcctgaaga tcctgctcac caacagctgc atcttcgact gccattactg
     5101 catcaaccgc aagagctcga acgtgcgccg cgcccgcttc accgcggagg aggtggtccg
     5161 gctcaccctc gccttctaca agcgcaacta tatcgagggg ctgttcctct cgtcggggat
     5221 catccgctcg tccgactaca cgatggagca gctggtcgag gtcgcgcgca gccttcgcga
     5281 ggaccatgat ttccgcggct acatccacct gaagaccatc cccgacgccg atccggagct
     5341 cgtgcgcctg gccggagtcc acgccgaccg ggtgtcgatc aacgtcgagc tgcccaccgc
     5401 cgcggggctc gcccggctgg cgcccgagaa ggacggggac aggatcgagg gcgcgatgaa
     5461 ggacatgcgc tcggccatcg aggagggcgc cgacgcgggg aagcgcttca gatcggcgcc
     5521 cgccttcgcg cccgccggcc agtcgaccca gatgatcgtc ggcgccgact ccgccaccga
     5581 cgccgacatc gtcggccggg ccagcgccct ctacgaccgc ttccgcctcc gccgggtcta
     5641 ttattcggcc ttctcgccca tccccgatgc ctcgtcggtc ctcccgctgc gccgtccgcc
     5701 gctgatgcgc gagcatcggc tctaccagtc cgactggctg atgcgcttct acggcttcgc
     5761 gcccggcgag gtggccgagg ccgccggcga cgacggcatg ctcccgctcg acatcgaccc
     5821 gaagctcgcc tgggcgctca agttccgcga gagcttcccg gtcgacgtca accgggcgcc
     5881 gcgggaagcc ctgctccggg tcccaggcct cggcgtcaag gcggtcgacc g
//
LOCUS       DASVQZ010000166         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_804552, whole genome shotgun sequence.
ACCESSION   DASVQZ010000166 DASVQZ010000000
VERSION     DASVQZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804552"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..75)
                     /locus_tag="VF547_04715"
     CDS             complement(<1..75)
                     /locus_tag="VF547_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEX8262156.1"
                     /translation="MVRPRPATFDKVKVVAENRRARFDY"
     gene            complement(221..1096)
                     /gene="dapA"
                     /locus_tag="VF547_04720"
     CDS             complement(221..1096)
                     /gene="dapA"
                     /locus_tag="VF547_04720"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX8262157.1"
                     /translation="MFRGSIPALVTIFRDEALDEHAFLDFVRWQVAEGSHALVPCGTT
                     GESATLTSAEHRRVIELAIEGAAGRVKVIAGCGSNNTAHAIELTRSAKEAGADAALHV
                     PPYYNRPNQEGIYAHLAAVTETVDIPIILYNVPARTITDISVETMARLAKIANVIGVK
                     DATGNLARVSAQRLACGEDFIQLSGNDDMALGFMAMGGRGCISVTANVAPRLCAEFQN
                     ACLSGDWQAALRFQDKLYPLHAALFTDASPGPVKYALGRVRPDLSSDLRLPMTPSSEA
                     SRKAVDEALKHAGLL"
     gene            1187..>2609
                     /locus_tag="VF547_04725"
     CDS             1187..>2609
                     /locus_tag="VF547_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8262158.1"
                     /translation="MQKKALFLSLLGVSAVSAAAVATGVRPVHVARMLPAAVQGAMTP
                     LPPPAQPRPAPAYAPVQSAVAADLARWNSLRQTDSLPFSSYASFLISHRGWPGETAMR
                     RTAEKANSDGGAAFEVIGFFSAFPPLTPAGHARHAFALQASGRGNEALEAARKAWTGG
                     VLPATDEARILSQFGGRLSSADHDARLEALLGNGDTQSAARTLPLASAQRQALYSTRL
                     ALQTKASDAASRLAALGPVEGDPGLLVDRANWLRNSGQSLAARQLMAQPRRLTRPPAN
                     AEKWFEAMMTLAKGAANDAQWSTAYQIASQLDDAYPAGTDVSIRPYGERDEYTNLAWL
                     AGTAANKLGRFADAARMFDRYGRAARSSQTRTKGFYWAARSAFNAGQKTQSDAWLEQA
                     AASPDQFYGQLALERLGRRVTPPSPTLASPTSAERSAFMTRPIVQATKALGDMDRWSD
                     QSLFVRAIAEQAETTGDRVLAAQF"
BASE COUNT          408 a          937 c          878 g          386 t
ORIGIN      
        1 ataatcgaag cgcgcgcgcc ggttctcggc gacgaccttg accttgtcga aggtggcggg
       61 acgggggcgg accatcaaat tcctcccctg catttgcagg ggagggggac catgcgtagc
      121 atggtggagg ggcgttgcgg cgccgcagaa cccctccacc gcgcgtcgcg cggtccccct
      181 ccccgagaaa actcggggag gaatttacga tacgaaccgc tcacagcaac cccgcatgct
      241 tcagcgcctc gtcgaccgcc ttgcggctgg cctcggaaga cggggtcatc ggcaagcgca
      301 ggtcggacga gaggtccggg cgaacgcggc ccagggcata tttgaccggc cctggggacg
      361 cgtcggtgaa gagcgcggca tggagcggat agagtttgtc ctggaatctc aatgccgcct
      421 gccaatcgcc tgataaacaa gcattctgga attcggcgca cagtctcggc gcgacgttcg
      481 cggtgaccga gatgcagccc cggccgccca tcgccatgaa gccgagcgcc atgtcgtcat
      541 tccccgaaag ctggatgaaa tcctcaccgc aggcgaggcg ctgggcggag acgcgggcga
      601 ggttgccggt cgcgtccttg actccgatca cgttggcgat cttcgccagc cgggccatcg
      661 tctcgaccga gatgtcggtg atcgtccggg ccgggacgtt gtagaggatg atcgggatgt
      721 cgaccgtctc ggtgaccgcc gccagatggg cgtagatgcc ctcctggttg gggcggttgt
      781 aataaggcgg gacgtggagc gcggcgtcgg cgcccgcctc cttcgccgag cgggtgagct
      841 cgatggcgtg ggcggtgttg ttggagccgc agccggcgat caccttcacc cgcccggcgg
      901 cgccctcgat cgccagctcg atcacccgcc ggtgctcggc cgaggtcagg gtcgccgatt
      961 cgccggtggt gccgcagggc acgagcgcat ggcttccctc agccacctgc cagcggacga
     1021 agtcgagaaa agcgtgctcg tccaacgctt cgtcgcggaa aatcgtcacc agagccggga
     1081 tcgacccgcg gaacatcaga agactccttc ccgtgaggct gcaggccgtc aataaggagg
     1141 cagccgttca gggcctgata aggagggggt tggcattctg tccagcatgc agaaaaaagc
     1201 gttgttcctg agcctcttgg gcgtgtccgc cgtatccgcc gcggcggtcg ccacgggcgt
     1261 tcggccggtc catgtcgctc ggatgctgcc cgccgcggtc cagggcgcga tgacccccct
     1321 cccgccgccg gcgcagcctc gcccggcgcc ggcctatgcg ccggtgcagt cggcggtcgc
     1381 cgccgacctc gcgcgctgga acagcctcag gcagaccgac agcctgccct tctcttccta
     1441 tgcttccttc ctgatcagcc atcgcggctg gcccggcgag acggcgatgc gccggaccgc
     1501 ggagaaggcc aattccgacg gcggcgccgc cttcgaggtg atcggcttct tcagcgcctt
     1561 tccgccgctc acccccgccg gccacgcccg ccacgccttc gccctgcagg cgtccgggcg
     1621 cggcaacgag gcgctcgagg cggcgcgcaa ggcctggacc ggcggagtcc tgccggcgac
     1681 cgacgaggcc cggatcctca gccagttcgg cggccgcctg agcagcgccg accatgacgc
     1741 ccggctcgag gccctgctcg gcaacggcga tacccagagc gcggcccgga ccctccctct
     1801 cgcctcggcc cagcgccagg cgctctactc gacccggctc gcgcttcaga ccaaggcgtc
     1861 cgacgcggcg agccgactcg ccgcgctcgg gccggtcgaa ggcgatcccg gactcctggt
     1921 cgaccgcgcc aactggctgc gcaattcggg ccagagcctc gccgcccgcc agttgatggc
     1981 gcagcctcgc cggctgaccc ggccgccggc caatgccgag aaatggttcg aggcgatgat
     2041 gacgctcgcc aagggcgcgg ccaatgacgc gcaatggtcg accgcctacc agatcgcctc
     2101 gcagctcgac gacgcctatc cggccggaac cgacgtcagc atccgtccct atggcgagcg
     2161 cgacgaatat accaacctcg cctggctcgc cggaacggcc gccaacaagc tcggccggtt
     2221 cgccgacgcg gcgcggatgt tcgaccgcta cggccgcgcc gcgcgctcgt cgcagacccg
     2281 caccaaggga ttctactggg cggcccgatc cgcgttcaac gccggccaga agacccagtc
     2341 cgacgcctgg ctcgagcagg cggcggcgag cccggaccag ttttacggcc agctcgccct
     2401 ggaacggctt gggcggcggg tcactccccc ctcgcccacc ctcgcctctc cgacctcggc
     2461 cgagcgctcg gcgttcatga cgaggccgat cgtccaggcc acgaaggcgc tcggcgacat
     2521 ggaccggtgg agcgaccagt cgctgttcgt ccgggccatc gccgagcagg ccgagacgac
     2581 cggcgaccgc gtgctcgccg cccagttcg
//
LOCUS       DASVQZ010000167         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_515335, whole genome shotgun sequence.
ACCESSION   DASVQZ010000167 DASVQZ010000000
VERSION     DASVQZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515335"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..812)
                     /gene="radC"
                     /locus_tag="VF547_04730"
     CDS             complement(129..812)
                     /gene="radC"
                     /locus_tag="VF547_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX8262159.1"
                     /translation="MADESLDGRGHRARLRRRLFEGGPDALLEHELIEYLLALALPRR
                     DTKPLAKKLIHDFGGFGRLLSADGEAIMRVGEVSEGAAAAIKIAQAAALRLLESKVAD
                     QPVLGSWQALLDYLRADMGASPIERVRVLYLNARNMLIRNEPLSEGSVDEAAVYVREV
                     IRRALEYHATAMILVHNHPSGDPQPSQQDIRLTREIVEAARPLGISVHDHVIVGARTC
                     ASMRSLGLI"
     gene            921..>1541
                     /locus_tag="VF547_04735"
     CDS             921..>1541
                     /locus_tag="VF547_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262160.1"
                     /translation="MDDEPGVEVRRRPVKRRVALALLLVILLALAVAWTQRRPIAADY
                     IDRELARRNVQASYQVKRIGFRTQRIENLVIGDPRAPDLTARWVEIRLAWRLGAPKVK
                     LITARGVRLNARLADGRITLGQVDRLLPPPSGLPFRFPDQEVDVADAAIRFDTPAGRV
                     GLALEGRGNLADGFRGRMAAAAHRLSLGSCRIDRIKGRWTVGVADLR"
BASE COUNT          230 a          530 c          549 g          232 t
ORIGIN      
        1 ctccccgaga tttctcgggg agggggaccg cgcgaaagcg gtggaggggt tctttcggat
       61 tcagcagaac ccctccacca ggcttcgcct ggtccccctc cccggcaaat gccggggagg
      121 attttcggct agatcaagcc caatgagcgc atgctggcgc aggtcctggc gccgacgatg
      181 acatggtcgt ggaccgagat tccgagcggc cgcgccgcct cgacgatctc gcgggtgagg
      241 cggatgtcct gctggctcgg ctgcgggtcg ccgctcggat ggttgtggac caggatcatc
      301 gcggtggcgt gatactcgag cgcccgccgg atgacctcgc ggacgtagac ggccgcctcg
      361 tcgaccgacc cctccgacag cggctcgttg cggatcagca tgttcctggc gttgaggtag
      421 aggacccgca cccgctcgat cggcgaggcg cccatgtcgg cgcgaagata gtcgagcaag
      481 gcctgccagc tgccgagcac gggctggtcg gcgaccttcg attcgagcag cctcagcgcc
      541 gcggcctggg cgatcttgat cgcggcggcc gcgccctcgc tgacctcgcc gacgcgcatg
      601 atcgcctcgc cgtcggcgga gagcagccgg ccgaagccgc cgaaatcgtg gatcagcttc
      661 ttggcgagcg gcttggtgtc ccggcgcggc agcgcgagcg caagcagata ttcaataagc
      721 tcatgctcca ggagagcgtc cggcccgccc tcgaacaggc gtctcctcag ccgcgcccga
      781 tgcccgcggc cgtcgagaga ctcgtcggcc atcagcaaag attaagcttc gcgggggcct
      841 taggcaaacc gcttgacccc gggccttcgg aaaggtgacg gaggcggaac ggaagcggag
      901 cgacggaggt tcaggagccg gtggacgacg agcccggagt ggaggtgcgg aggcggccgg
      961 tgaagcggcg ggtcgcgctc gccttgctcc tcgtcatcct tctcgcgctc gccgtcgcct
     1021 ggacccagcg ccggccgatc gccgccgact atatcgaccg cgaactcgcc cgccgcaacg
     1081 tccaggccag ctatcaggtc aagcggatcg gctttcgcac ccagcggatc gagaatctcg
     1141 tcatcggcga tccgcgcgca cccgatctca cggcgcgctg ggtcgagatc cggctggcct
     1201 ggcgcctcgg cgctccgaag gtgaagctga tcaccgcgcg cggagtccgc ctcaacgccc
     1261 ggttggcgga cggccggatc acgctcgggc aggtcgaccg gctgctgccg cccccgtccg
     1321 gcctgccgtt ccgctttccc gaccaggagg tcgacgtcgc cgacgccgcg attcgcttcg
     1381 acacgccggc cggccgggtc gggctcgcgc tcgagggccg cggcaatctc gccgacggct
     1441 tccggggccg gatggcggcg gcggcgcacc ggctgagcct gggaagctgc cggatcgacc
     1501 gcatcaaggg gcggtggacg gtcggcgtcg cggacctgag g
//
LOCUS       DASVQZ010000168         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_612332, whole genome shotgun sequence.
ACCESSION   DASVQZ010000168 DASVQZ010000000
VERSION     DASVQZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612332"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF547_04740"
     CDS             complement(<1..272)
                     /locus_tag="VF547_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HEX8262161.1"
                     /translation="MKRAFLAMALMLAAPLTPASAQHVVNFREADIRAFIEDAARVTG
                     RTFIVDPAVNGKVSVVTQRPLSRSEYFELFLSTLRANGLMAVPLQGG"
     gene            complement(269..877)
                     /locus_tag="VF547_04745"
     CDS             complement(269..877)
                     /locus_tag="VF547_04745"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX8262162.1"
                     /translation="MRLAFDSRRLIGRARGVNGWTMLEFALLGLVALQCARLIWTALT
                     PVGPLGDWRGSATGPGPATIGILGGFDPFFRLAGAAGPVTVTSLNLKLFGIRQDQASG
                     RGSAIIQGSDGQQRSIAVGEEIEPGVILKSVDFDSVTISRGGSDEQLFMDQSQGGTTA
                     APPPGPAAPAAPVTQPSTAVEPGGPPVPPSKLYPNEKPAGVE"
     gene            complement(1029..1790)
                     /locus_tag="VF547_04750"
     CDS             complement(1029..1790)
                     /locus_tag="VF547_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8262163.1"
                     /translation="MTALPAPAEAAWTALAFILGAAIGSFLATILVRWPQGRSVVAGR
                     SACDSCGRTLEARDLVPILSWSLARGRCRHCGGRIDRRHLAAEAGAAMVAIAAILVHP
                     MPMAAVTAILGWWLFLIAAMDLEHEWLPNRLTWPLLVAGLAAAWGGFGPSFESRLIGA
                     AAGFASLFLVALAYRTLRGREGLGGGDPKLFAAIGAWLGWTQLPFVLVGAGLLGLAAL
                     LLKRLRGGSVAATDRLPLGTLMALAAWPIWIVISR"
     gene            complement(1787..>2376)
                     /locus_tag="VF547_04755"
     CDS             complement(1787..>2376)
                     /locus_tag="VF547_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX8262164.1"
                     /translation="IVLPKAEGGASVAELTRRLTGAGNVTAQILAIATETPAAMFQLG
                     TYGGLKRLAGLTWGAEDLPAAIGAATSREADGRYTAPYELARSLCLFGAAAAGVAPIE
                     TVFPAFRDLEGLAAYAGRARRDGFTGMMAIHPDQVPVINAAFTPSEAEVAHARAVVAA
                     FEANPGAGTLALDGKMIDRPHLVQAQRTLAAAAGS"
BASE COUNT          387 a          838 c          842 g          309 t
ORIGIN      
        1 ccgccctgga gcggcaccgc catcagcccg ttggcgcgga gggttgaaag gaacagctcg
       61 aaatattcgg aacgcgaaag cggccgctgg gtgacgacgg agactttgcc gttcaccgcc
      121 gggtcgacga tgaaggtccg cccggtcacc cgggcggcat cctcgatgaa ggcccgaatg
      181 tcggcctcgc ggaaattgac gacatgctgg gccgaggcgg gcgtcagcgg cgcggcgagc
      241 atcagggcca ttgcgaggaa ggcgcgcttc attcgacgcc cgccggcttt tcgttgggat
      301 agagcttgga gggcggaacg ggcggaccgc ccggctcgac ggcggtgctc ggctgggtga
      361 cgggcgcggc cggagcggcg ggaccgggcg gcggagccgc cgtcgtgccg ccttgcgact
      421 ggtccatgaa caattgctcg tccgatcctc cgcgcgagat ggtgacgctg tcgaaatcga
      481 ccgacttcag gatcactccg ggctcgatct cctcgccgac ggcgatgctg cgctgctggc
      541 cgtccgagcc ctggatgatc gccgagcccc ggcccgaagc ctggtcctgc cggattccga
      601 acagcttcag gttgagcgag gtgacggtta ccggcccagc cgcgccggcg agacggaaga
      661 agggatcgaa gccgcccaat atgccgatgg tcgcgggccc cgggccggtg gcgctgccgc
      721 gccagtcgcc gagcgggccc accggagtga gggcggtcca gatcagccgg gcgcattgca
      781 gggcgacgag gccgaggagc gcgaattcga gcatggtcca gccgttgacg ccccgggcgc
      841 ggccgatcag ccgccggctg tcaaaagcta aacgcatcga aatcgaaccc cttccccgcg
      901 tctaccctga cgcagcagat tttccacagt cataacaatg ttttggcgac catccagccc
      961 gagattcgcc agccgggcaa acttccagct tttgcggccc cgcgcaagcg gcttgtgaga
     1021 cgcggcgatt accggctgat gacaatccag atcggccagg cggcaagggc catgagggtg
     1081 ccgagcggca accggtccgt cgccgccacc gatccgccgc gaagccgctt caggagcagc
     1141 gccgcaagtc ccagcagccc cgccccgacc agcacgaacg gcaattgagt ccagcccagc
     1201 caggcgccga tcgccgcgaa cagcttgggg tcgccgccgc cgagcccctc ccgtccgcgc
     1261 agcgtccggt aggcgagagc gacgaggaac agcgacgcga agccggcggc ggcgccgatc
     1321 agccggcttt cgaaggacgg gccgaagccg ccccaggccg ccgccagccc ggccacgagg
     1381 agcggccagg tcagccggtt gggcagccat tcatgctcaa ggtccatcgc cgcgatcagg
     1441 aacagccacc agcccaggat cgcggtcacg gcggccatcg gcatcgggtg gacgaggatc
     1501 gccgcgatcg ccaccatcgc ggctccggcc tcggcggcga ggtgccggcg gtcgatccgg
     1561 ccgccgcaat ggcggcagcg gcccctggcc agcgaccagc tcaggatcgg caccaggtcg
     1621 cgggcctcca gggttcttcc gcacgagtcg caggccgacc ggcccgccac cacgctccgc
     1681 ccctgcggcc agcgcaccag gatggtcgcg agaaagctcc cgatggccgc gccgaggatg
     1741 aaagcgagcg cggtccaggc ggcttccgcc ggcgccggca gggcggtcac gaccccgcgg
     1801 cggcggcgag ggttcgctgc gcctggacga ggtgcggccg gtcgatcatc ttcccgtcga
     1861 gcgccagcgt gcccgctccc gggttcgcct cgaacgcggc gaccacggct cgggcatgcg
     1921 ccacctcggc ctcgcttgga gtgaaggcgg cgttgatcac cggcacctga tcggggtgga
     1981 tcgccatcat cccagtgaat ccgtcgcgcc tcgcccgtcc ggcataggcg gcgagacctt
     2041 cgagatcgcg gaaggccggg aagacggtct cgatcggagc gactcccgcc gcggccgcgc
     2101 cgaacaggca gagcgaccgg gcgagctcat agggcgcggt ataacggccg tccgcctcgc
     2161 gcgaggtcgc cgctccgatc gcggccggca ggtcctcagc gccccaggtg agtccggcga
     2221 gccgcttgag cccgccatag gtgccgagct ggaacatcgc cgccggagtt tccgtggcga
     2281 tggcgaggat ctgggcggtg acgttgccgg cccccgtcag cctccgagtc agctcggcga
     2341 cgctcgcccc gccctccgcc ttgggaagaa cgatcc
//
LOCUS       DASVQZ010000169         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_135710, whole genome shotgun sequence.
ACCESSION   DASVQZ010000169 DASVQZ010000000
VERSION     DASVQZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135710"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..830)
                     /locus_tag="VF547_04760"
     CDS             complement(240..830)
                     /locus_tag="VF547_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX8262165.1"
                     /translation="MAKILGNFHLALLLGLVLLAAAMFGLHGALLGDGSYPWAQWLRF
                     VHVFAGILWIGLLYYFNFVQIPTMPSVPAELKPGVTKYIAPRALFWFRWSALATVILG
                     LLVAETTQGGYSARALTLADGYKTIGLGMWLGLIMAANVWFVIWPNQKKALGIVPADD
                     AAKAKAGSIAMMASRTNLILSIPMLYCMVAQTHIGG"
     gene            complement(1024..1698)
                     /locus_tag="VF547_04765"
     CDS             complement(1024..1698)
                     /locus_tag="VF547_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HEX8262166.1"
                     /translation="MRRALRIALRAAAALLAVPAFYFLAALVLGAVPANRGWREAKEG
                     VTIFVRTNGVHTWILVPKVTPEMDWRPLVPGAHLADPRWGRGDYVALGYGNRTFYLET
                     PTWGDLSMKNAFLAAFGRGRTLMHADHDHDPRPSEDTRPIVLRRDEYRRLVQFARASF
                     QYDSNGRTMPLIGRGYGPSDMFYEAVGPYSVVYTCNSWTGRALREAGVRTGVWTPLSQ
                     SIMWRL"
     gene            1719..2930
                     /locus_tag="VF547_04770"
     CDS             1719..2930
                     /locus_tag="VF547_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX8262167.1"
                     /translation="MRRLRSAKPDAMSREHLEAIWRAGVEACLPARVVPPHLPEPPPG
                     RTLLLALGKAAIPMAAAVEARWPGPLAGLAVAPRGSPGALRRIEVIPSEHPVPSSGSV
                     AAARRLLALAGAASADDLVLVLLSGGASSLACLPGEGLALREKAGLTEALLRSGATVR
                     EINCVRRHLSAFKGGRLALAAAPARLVTLAISDVVGDRPEDIGSGPTAADPSTQGEAR
                     SILERHSIAAPEGGWSETPTRVPGDYRIVARGADALAAAAAEAERLGYKARRLECEGE
                     ARDVGRAHAALALAAAPGTALISGGELTVTVRGAGIGGPNQEYALAATLALAGRGGIR
                     GLAADSDGLDGRSHAAGAFFEGGGDTSGARAALDANDSTPWFERRGGLLVTGATGTNV
                     NDLRIILAGPA"
     gene            3097..>3452
                     /locus_tag="VF547_04775"
     CDS             3097..>3452
                     /locus_tag="VF547_04775"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403206.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX8262168.1"
                     /translation="MGEWTIGILGGSGLYAMEALEEAQWVSVDTPWGAPSDQLLIGRL
                     HGVRFVFLPRHGRGHRLPPSQVNARANVDALKRAGCTDLVAISAVGSLREALEPGRFV
                     LVDQFIDRTFAREKSF"
BASE COUNT          498 a         1186 c         1285 g          483 t
ORIGIN      
        1 ccggattgtc ggagagcaat ttcgcgacgt gcagccgtcc gtccggcgcc cgggcgacga
       61 cgtcggtgaa cgtgccgccg cggtcgatcg agaaggacca gcccttcgtc atccgcggcc
      121 gtcacccgtc attgcgggcg aagcgaagca atccagcggc gcaagcctgc gagtctggat
      181 tgcttcgtca cttcgttcct cgcaatgacg gaggtgagcc tcgtccccgg cttgccggct
      241 cagccgccga tgtgggtctg ggcgaccatg cagtagagca tcgggatcga gaggatgagg
      301 ttggtccggc tcgccatcat cgcgatcgag ccggccttcg ccttggcggc atcgtcggcg
      361 gggacgattc cgagcgcctt cttctggttc ggccagatca cgaaccagac attggcggcc
      421 atgatcaggc ccagccacat gcccaggccg atcgtcttgt agccgtcggc gagcgtcaag
      481 gcgcgggcgc tgtagccgcc ctgcgtcgtc tcggcgacga gcagtccgag gatcacggtc
      541 gccagcgccg accagcggaa ccagaacagc gccctgggcg cgatatattt ggtgacgccc
      601 ggcttcagct cggccgggac gctcggcatg gtcgggatct ggacgaagtt gaaatagtag
      661 aggaggccga tccagaggat gccggcgaag acgtggacga agcgaagcca ttgcgcccac
      721 ggataggagc cgtcgccgag cagcgccccg tgcagcccga acatcgccgc ggccagaagc
      781 accaggccga gcagaagcgc gaggtggaag ttgccgagaa tcttcgccat cgaaaccccc
      841 tgtggtttgt cggttgggcg cccctgtaac gcagggggaa ggcggagggt aggtgaaaat
      901 cctccccagg atttcctggg gagggggacc aggcgaagcc tggtggaggg gttctccaga
      961 ctccgcagaa cccctccacc gcgccccgcg cggtccccct ccccgcgaaa tcgcggggag
     1021 gatttagagc cgccacatga tgctctgcga gaggggggtc cagacgcccg tcctcacccc
     1081 cgcctcgcgc agcgcccggc cggtccagct gttgcacgtg tagacgacgc tgtacggccc
     1141 caccgcctcg tagaacatgt cggaggggcc gtagccgcgg ccgatcagcg gcatcgtccg
     1201 gccattcgag tcatattgga agctggcgcg ggcgaactgg acgaggcgtc gatattcgtc
     1261 gcggcgaagc acgatcggcc gggtatcctc gctcggccgc ggatcgtggt cgtgatcggc
     1321 gtgcatcaag gtgcggcccc ggccgaaggc ggcgaggaag gcgttcttca tgctgaggtc
     1381 gccccaggtc ggcgtctcga ggtagaaggt gcggttgccg tagcccaggg cgacgtagtc
     1441 gccccggccc cagcgcggat ccgcgagatg ggcgccgggg acgagcggcc gccaatccat
     1501 ctcgggcgtc accttgggca ccaggatcca ggtgtggacg ccgttggtcc ggacgaagat
     1561 cgtcaccccc tccttcgcct cccgccagcc gcgattggcg gggacggcgc cgaggaccag
     1621 ggcggcgagg aaatagaaag cggggacggc gagcagggcg gcggcggcgc gaagggcgat
     1681 gcggagggcg cgtctcacgc cgaggagcct agcgcttgat gcggcgcctg cgaagcgcca
     1741 agccggatgc catgtcgcgc gagcatctgg aagcgatctg gcgggcgggg gtggaggcgt
     1801 gcctgccggc gcgggtggtg ccgccgcacc ttccggagcc gccgcccggc cgaaccctcc
     1861 tgctcgcgct cggcaaggcg gcgatcccga tggcggcggc ggtcgaggct cggtggccgg
     1921 gaccgctggc ggggctcgcg gtggcgcccc ggggcagccc gggcgcgctc cggcggatcg
     1981 aggtcatccc ttccgagcac ccggtcccct cttccggcag cgtcgccgcg gcccggcggc
     2041 tgctggcgct ggccggtgcg gcgtcggcgg acgacctggt cctggtgctg ctgagcggag
     2101 gcgcctcgtc gctcgcctgc ctgcccggcg aaggcctcgc gctgcgggag aaggcggggc
     2161 tgaccgaggc tctgctccgc tccggcgcga cggtccgcga gatcaactgc gtccgccgcc
     2221 acctctcggc gttcaagggc ggccggctgg cgctggcggc ggctccggcg cggctcgtca
     2281 ccctcgccat ctccgacgtg gtcggcgacc ggcccgagga catcggctcg ggcccgaccg
     2341 ccgccgatcc ctcgacccaa ggggaggcgc gctcgatcct cgagcgccat tcgatcgcgg
     2401 cgcccgaggg gggctggagc gagactccga cgcgcgtgcc cggcgactat cggatcgtcg
     2461 cgcgcggcgc ggacgcgctg gccgcggccg ccgccgaggc ggagcggctc ggctacaagg
     2521 cgcgcaggct cgaatgcgag ggggaggcgc gcgacgtcgg gcgcgcgcat gccgccctcg
     2581 ccctggcggc cgcgcccggc accgcgctga tctcgggcgg cgagctcacc gtcaccgtcc
     2641 gcggcgcggg catcggcggc cccaaccagg aatatgcgct cgccgcgacg ctggccctgg
     2701 ccggccgcgg cgggatccgc gggctcgccg ccgacagcga cggactcgac ggccgaagcc
     2761 acgcggccgg cgccttcttc gagggcggcg gcgacacgag cggcgcgcgg gcggcgctcg
     2821 acgccaacga ttcgaccccc tggttcgagc ggcggggcgg cctgctcgtc accggcgcca
     2881 ccggcaccaa cgtcaacgac ctcaggatca tcctcgccgg ccccgcctga gatcgagcct
     2941 cctcatatcc aatgcccgca tgttcggtgc ctggcaccga ggctcaaggg gttgggatcg
     3001 cttggcgggg gtggggtgcg ggatcgggga atcggatgcg gaggggcggt ccgcgcggcc
     3061 ctcgtcaaag cgaggcggcg ctggtaccgc cgggcgatgg gcgaatggac gatcggaatc
     3121 ctcggcggct cggggctcta cgcgatggag gcgctcgagg aggcgcaatg ggtctcggtc
     3181 gacaccccct ggggggcgcc gtccgaccaa ttgctgatcg ggcggctgca cggagtcagg
     3241 ttcgtcttcc tgccccgcca cggccgcggc catcgccttc cgcccagcca ggtcaacgcc
     3301 cgcgccaatg tcgacgcgct gaagcgggcg ggatgcaccg acctcgtcgc catctcggcg
     3361 gtcgggtcgc tgagggaggc gctcgagccc ggccgattcg tcctcgtcga ccagttcatc
     3421 gaccggacct tcgcccgcga gaagagcttc tt
//
LOCUS       DASVQZ010000170         4489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_230587, whole genome shotgun sequence.
ACCESSION   DASVQZ010000170 DASVQZ010000000
VERSION     DASVQZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230587"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF547_04780"
     CDS             <1..231
                     /locus_tag="VF547_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HEX8262169.1"
                     /translation="EHQVSDPLIVAWRAMTVALLDRMAPLVRARLGVAEEDFPLARML
                     EGGSWAAGRRIAAERRPGGGPPFTIISDGTVF"
     gene            232..870
                     /gene="upp"
                     /locus_tag="VF547_04785"
     CDS             232..870
                     /gene="upp"
                     /locus_tag="VF547_04785"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX8262170.1"
                     /translation="MDAIEGLTIVSHPLVQHKLTLLRDRNSSIQLFRSVVREIATLLC
                     YEVTRDLPTDLVSIETPIATTQSHAIAGKKLVIAPILRAGLAMAEGMLDLLPSARVAH
                     IGLYREPVTLQCVEYYFKTPEDCAERLVIVVDPMMATGHTAIAAIDRLKEAGVADMRF
                     VCLLASRPGAEAVRAAHPDVPIWAAAMDEKLNDHGYIVPGLGDAGDRTYGTR"
     gene            complement(901..1731)
                     /locus_tag="VF547_04790"
     CDS             complement(901..1731)
                     /locus_tag="VF547_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262171.1"
                     /translation="MTQGRAGPPAGAEAGDDPGTRPCRACWQPMPEQANKCTQCGSVQ
                     NWERYLGFSGTILGLLVALISVLTFALPIWSGLIIPRAARPSAVLVEVDDSGRATFTV
                     SNGGTAPAAIEQIVVGRGDGVAEFDLGGLPGSERVVQPNDVRIFNARLHVTNEAEDVR
                     HLASMIRANSKCPTAIWVVTPAGSRETARVVTTTPAEEDPDEPGCGFKLRKLFLTTLI
                     HRCSDPAFEAALLKAEAAPRAVPGPSLHCPLAGKPDRPENRASDDSGGPPVEAAPGGR
                     "
     gene            complement(1986..2837)
                     /locus_tag="VF547_04795"
     CDS             complement(1986..2837)
                     /locus_tag="VF547_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262172.1"
                     /translation="MSPALRPWLAALAAILGAPAGAVTPEQAQALPVGELARLVLGEA
                     GALMVDVDRPKWPACRMIACPFYTEEQLKRPPPLRFGLTFYARPFAALDIANRWTGAC
                     GVLILDVTFDERDELTGIGTRVSWGVPHGLEPVPAAAASKDFPARLEAAGEKCRAGAD
                     PRSFFVSDSSDSAYRVAIAANRFAEAARRGKPLPFRFKCSSHRFQCDDGGAESVAARF
                     RPANIAQAMQVDCAEPHLLMTTIGPAGCYEVRFREPGETLLVEVADAYSVIRIVRVEY
                     THSTVVY"
     gene            complement(2834..>4489)
                     /gene="acs"
                     /locus_tag="VF547_04800"
     CDS             complement(2834..>4489)
                     /gene="acs"
                     /locus_tag="VF547_04800"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX8262173.1"
                     /translation="LFEGDEPGSGRSLTYGGLHREVVAMANALKALGAGKGDRVTLYM
                     PMIPEAAVAMLACARIGAVHSVVFGGFSPEALHGRIEDCASRFVVTADGGKRGGRLVP
                     LKANVDAALAKGAEVEAVLVVRHLGNEIGWTEGRDHWLHDLAGLVPDDCPCDPMNAED
                     PLFILYTSGSTGKPKGVLHTTGGYAVWTAATFHYVFDYKPGEIFWCTADVGWVTGHSY
                     VVYGPLANGATTLMFEGVPNYPDFSRFWDVVERRKVDIFYTAPTAIRALMREGEGPVR
                     KHDRRSIRLLGTVGEPINPEAWRWYHEVVGESRCPIVDTWWQTETGGVMITTLPGAHD
                     MKPGSAGRPFFGVEPQLVGPDGEVLEGAASGNLCLARSWPGQMRTVYGDHDRFVQTYF
                     STYRGKYFTGDGCRRDEDGYYWITGRVDDVLNISGHRLGTAEIESALVSHPKVAEAAV
                     VGYPHDIKGQGIYCYVTLMEGEEGSEALERELRQHVRTEISPIASPDLIHFTPGLPKT
                     RSGKIMRRILRKIAENDFGSLGDTSTLADPALVDGLIEGRRNK"
BASE COUNT          660 a         1554 c         1584 g          691 t
ORIGIN      
        1 gagcaccagg tttccgaccc gctgatcgtc gcctggcggg cgatgaccgt ggctttgctc
       61 gaccgcatgg cgccactggt acgcgcgcgg ctcggcgttg cggaggaaga cttcccgctc
      121 gcccgcatgc tcgagggcgg cagctgggcg gccggacgcc ggatcgccgc cgagcgccgg
      181 ccgggcggcg ggcccccttt caccatcatc agcgacggga cggtattcta gatggacgca
      241 atcgaaggac tgaccatcgt ctcgcacccc ctggtgcagc acaagctcac gctcctcagg
      301 gaccgcaaca gctcgatcca gctgttccgc tcggtggtgc gcgagatcgc caccctgctc
      361 tgctacgagg tcacccgcga cctgccgacc gacctcgtca gcatcgagac tccgatcgcg
      421 accacccagt cgcacgccat cgccggcaag aagctggtca tcgccccgat ccttcgcgcc
      481 ggcctcgcca tggccgaggg catgctcgac ctgctcccct ccgcccgggt cgcccatatc
      541 ggcctctacc gcgagccggt gacgctccag tgcgtcgaat attatttcaa gacgccagag
      601 gattgcgccg agcggctggt catcgtcgtc gacccgatga tggcgacggg ccacaccgcg
      661 atcgcggcga tcgaccggct caaggaggcc ggcgtcgccg acatgcgctt cgtctgcctg
      721 ctcgcctcgc gccccggcgc cgaggcggtc cgggccgccc atcccgacgt gccgatctgg
      781 gcggcggcga tggacgagaa gctcaacgac cacggctata tcgtgccggg cctcggcgat
      841 gcgggcgacc gcacctacgg cacccgctga aggcgttcac cgccgcgcgc cgataacaat
      901 tcaccgaccg ccgggcgcag cctcgacggg cggaccgccg ctgtcgtccg aagcgcggtt
      961 ttccggccta tcgggcttcc cggcgagggg gcaatggagg ctcggccccg gaacggcccg
     1021 aggcgcggct tcggccttga ggagcgcggc ctcgaaggcc gggtcgctgc agcggtggat
     1081 gagcgtggtg aggaacagct tgcgaagctt gaacccgcat cccggctcgt cggggtcttc
     1141 ctcggcgggc gtcgtcgtca cgaccctggc ggtctcgcgg ctgccggcgg gggtgacgac
     1201 ccagatcgcg gtcgggcatt tcgaattggc gcggatcatg ctggcgaggt ggcggacgtc
     1261 ctccgcctcg ttggtgacgt gcagccgggc gttgaagatc ctgacatcgt tgggctggac
     1321 gacccgctcg ctgccgggca ggccgccgag gtcgaactcc gccaccccgt cgccgcggcc
     1381 gacgacgatc tgctcgatcg ccgccggcgc cgtgcccccg ttcgacaccg tgaaggtggc
     1441 ccggccgctg tcgtcgacct cgacgaggac ggcgctcggc cgcgcggccc tcgggatgat
     1501 cagcccgctc cagatcggga gggcgaaggt gagaacgctg atcagcgcga ccagcaggcc
     1561 gaggatcgtg ccgctgaagc cgagataccg ctcccagttc tggacgctgc cgcattgcgt
     1621 gcatttgttc gcctgctccg gcatgggctg ccagcaggcg cggcacggcc tggtgccggg
     1681 atcgtcgccg gcttcggccc cggcgggagg gcccgcccga ccctgcgtca tcgtcccctc
     1741 ctccttcgag ctgagcctgg accgcgaccg ccatcatgcc tgcgatcgag cgccaagacc
     1801 atccggccat tcggattgcc gcgagccgag aaaacggcag cgaccctcct gcgcggcgtc
     1861 gtggaggtca aatcgcgtcg gccggcgaat tcgggcattg ccttgcctcc gacgcgatgg
     1921 acagccgcaa ggggcggtgg aggtagaggg cggcgaagct tcgcaatgac gaaggcggcc
     1981 gccgctcagt agacgaccgt gctgtgcgta tattcgaccc ggacgatcct gataaccgaa
     2041 taggcgtcgg cgacttcgac cagcagggtc tcccccggct cgcggaaccg gacctcgtag
     2101 cagccggcgg ggccgatggt ggtcatcagc aggtggggct cggcgcagtc gacctgcatc
     2161 gcctgcgcga tgttcgccgg ccggaaccgg gccgcgaccg actcggctcc gccgtcgtcg
     2221 cattggaacc gatgggagga gcatttgaac ctgaagggca gcggcttccc gcgccgggcc
     2281 gcttccgcga atcgattcgc cgcaatcgcc acccggtagg cgctgtcgct ggagtcggag
     2341 acgaagaagc tgcgcggatc ggcgccggcg cggcacttct cgcccgccgc ctcgagccgc
     2401 gccgggaagt ccttcgaagc cgccgccgcg ggcaccggct cgagcccgtg cggcacgccc
     2461 cagcttaccc gagtcccgat tcccgtcagc tcgtcacgct cgtcgaaggt cacgtcgagg
     2521 atgagcacgc cgcacgctcc ggtccatcgg ttggcgatgt ccaaggccgc gaagggacgc
     2581 gcatagaaag tcaggccgaa cctgagaggg ggcggccttt tcagctgctc ctcggtgtag
     2641 aaggggcagg cgatcatccg gcaggccggc catttcggcc tgtcgacgtc caccatcagc
     2701 gctccggcct cgccgagcac gagccgcgcc agctcgccga cgggaagggc ctgggcctgc
     2761 tccggcgtga ccgcgcccgc cggggcgccg aggatcgcgg ccagcgcggc gagccatggc
     2821 cgcagtgccg ggctcacttg ttgcgccgcc cttcgatcag gccgtcgacg agcgccgggt
     2881 cggccagggt gctcgtgtcg ccgaggcttc cgaaatcgtt ctcggcgatc ttgcgcagga
     2941 tccggcgcat gatcttgccc gagcgggtct tgggaaggcc cggggtgaag tggatgaggt
     3001 cggggctggc gatcgggctg atctccgtcc ggacgtgctg gcgcagctcc ctctccagcg
     3061 cctcgctgcc ctcctccccc tccatcaggg tgacgtagca atagatgccc tggcccttga
     3121 tgtcgtgcgg atagccgacg accgcggcct cggcgacctt ggggtgcgag accagggcgc
     3181 tctcgatctc ggcggtgccg aggcggtggc cggagatgtt gaggacgtcg tcgacccggc
     3241 cggtgatcca gtaatagccg tcctcgtcgc ggcggcagcc gtcgccggtg aaatatttgc
     3301 cgcggtaggt cgagaaatag gtctgaacga agcgatcgtg atcgccatag acggtgcgca
     3361 tctggccggg ccaggagcgg gccaggcaga ggttgccgga ggccgcgcct tcgagcacct
     3421 cgccgtcggg accgaccagc tgcggctcga cgccgaagaa ggggcggccg gcgctccccg
     3481 gcttcatgtc gtgggcgccg ggcagggtcg tgatcatcac tccgcccgtc tcggtctgcc
     3541 accaggtgtc gacgatcggg cagcggcttt cgcccacgac ttcgtgatac cagcgccagg
     3601 cctccgggtt gatcggctcg ccgaccgtcc cgagcaggcg gatcgagcgg cggtcgtgct
     3661 ttcggaccgg gccctcccct tcgcgcatca gcgcccggat ggccgtcggg gcggtgtaga
     3721 agatgtcgac cttgcggcgc tcgacgacgt cccagaagcg gctgaaatcg ggatagttgg
     3781 gaacgccctc gaacatcagg gtcgtcgcgc cgttggcgag cgggccgtag acgacgtagc
     3841 tgtgcccggt gacccagccg acgtcggcgg tgcaccagaa gatctcgccg ggcttgtagt
     3901 cgaacacgta gtggaaggtc gccgccgtcc agacggcata gccgccggtg gtgtggagca
     3961 cgcccttggg ctttccggtc gagcctgacg tgtagaggat gaacaagggg tcctcggcgt
     4021 tcatcggatc gcacgggcag tcgtccggga cgagcccggc caggtcgtgg agccaatggt
     4081 cgcggccttc ggtccagccg atctcgttgc cgaggtggcg gacgacgagc accgcctcga
     4141 cctcggcgcc cttggcgagg gcggcgtcga cattggcctt caagggcacc agcctgccgc
     4201 cgcgcttgcc gccgtcggcg gtgacgacga agcggctcgc gcaatcctcg atccggccgt
     4261 ggagcgcctc cggcgagaag ccgccgaaga cgaccgaatg gacggcgccg atgcgggcgc
     4321 aggcgagcat cgcgaccgcc gcctcgggga tcatcggcat gtagagggtg acccggtcgc
     4381 ccttgccggc gccgagcgcc ttgagcgcgt tcgccatggc gacgacctcg cggtggagcc
     4441 cgccgtaggt caggctccgg ccgctgcccg gctcgtcgcc ttcgaacag
//
LOCUS       DASVQZ010000171         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_324565, whole genome shotgun sequence.
ACCESSION   DASVQZ010000171 DASVQZ010000000
VERSION     DASVQZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324565"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF547_04805"
     CDS             complement(<1..313)
                     /locus_tag="VF547_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262174.1"
                     /translation="MLRVALMGLAAAAAAAGSHGSARAAAPPAATLEASGFATILQAP
                     PSPFPDRLPPPDDRPKDEAGYYARMAGIGDAEARRRLAEQQAARPEFERLLGTLRARE
                     KG"
     gene            625..>1950
                     /locus_tag="VF547_04810"
     CDS             625..>1950
                     /locus_tag="VF547_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX8262175.1"
                     /translation="MANNYTFQVVDRDNLVSASIEASINNCAQYVIALISRYIEWKGT
                     IDFVVEIRPAAELTWSDADGLLPSITQVSWNGSAWVNDTLAECLTGFDSDPSRPDAGC
                     TIYLADDGTIKNYGVPVWFDPNPVFGSDPAVPAGMHDFVGILTHEIFHSLGFIHFTKE
                     WSDRIATQGGISYFTGTNAAALYGGPVPFQSGFDHYGYVQDPAIPISRGLMFQFGNYA
                     QNRFDIGRIDLAILADLGHTVKTYDGLGLFEFIDTATGLAGSEAGETLYGDYHSNALS
                     GLGGDDRIEGGSGDDLLRGDDGNDQLVGGAGNDVMLGGRGVDGFDGGGNEPFGPSSSF
                     GDQVSFLDSAATQGAVADLHTGIISNDGFGNAESMTGIESLRGTGLGDFLGGDAAANR
                     LVGEGGEDVIFGRGGDDIIEGGGGVDLLHGHEGDDSLSGGSGNDLLRGYA"
BASE COUNT          303 a          671 c          664 g          312 t
ORIGIN      
        1 tgcctttctc gcgggcgcga agcgtgccga gcagccgctc gaactcgggg cgcgccgcct
       61 gctgctcggc gaggcgcctg cgcgcctcgg cgtcgccgat ccccgccatc cgcgcataat
      121 agcccgcctc gtccttcggc cggtcgtcgg ggggcgggag ccggtcggga aagggggagg
      181 ggggcgcctg caggatggtg gcgaagccgg acgcctccag tgtcgcggcg ggaggggccg
      241 ccgcgcgggc ggacccgtgc gagcctgcgg ccgcggcggc cgccgccagc cccatcagcg
      301 cgaccctaag cattcctggc ttccacgcgc gattccctcc gcaaatgccg cggcagtcga
      361 tcacggcgcg gcttgctggg acctgaagga cgcagccttg cctggcctcg acgggctgcg
      421 ccggatcgga ccttggccgc gggcacctgc ggcagcgatc ggggacgccc gccagccgga
      481 tatgcttcgc ctgtgccggc tccgccgcaa ggagggcggg gttttcgcct tgtagttgtt
      541 caaccgtctg aataacgtcg ggaattgggt gcggtactcc cccggggggg cggctgattc
      601 gccgatcgca gcgggggaca tctcttggcg aacaattata cgttccaggt cgtcgaccgg
      661 gacaatctcg tctcggcctc gatcgaggcg agcatcaaca attgcgccca atatgtcatc
      721 gccctgatca gccgctacat cgagtggaag ggcacgatcg acttcgtcgt cgagatcagg
      781 ccggcggccg agctgacctg gtccgacgcg gatgggctgc ttccgagcat cacccaggtg
      841 agctggaacg gcagcgcgtg ggtcaacgac acgctcgctg aatgcctgac cgggttcgac
      901 tccgatccca gccgcccgga tgccggctgc acgatctatc tcgccgacga cggcaccatc
      961 aagaattacg gcgtgcccgt ctggttcgac cccaaccccg tcttcgggag cgatcccgcc
     1021 gtgcccgcgg gcatgcacga tttcgtcggg atcctcaccc acgagatctt ccattccctc
     1081 ggcttcattc acttcaccaa ggaatggtcc gaccggatcg cgacccaagg cggcatctcc
     1141 tatttcaccg ggacgaatgc cgccgccctg tacggcggtc ccgtgccgtt ccagagcggc
     1201 ttcgaccatt acggctacgt ccaggacccg gcgattccga tcagccgcgg gctgatgttc
     1261 cagttcggca attacgccca gaaccgcttc gacatcggcc gcatcgacct cgccatcctc
     1321 gccgacctcg gccacacggt gaaaacctat gacgggctgg ggttgttcga gttcatcgac
     1381 acggcgaccg gtctcgcagg atcggaggcc ggcgagacgc tctatggcga ctatcattcg
     1441 aacgcgctgt ccggactcgg cggcgacgac cggatcgagg gcggctcggg cgacgatctc
     1501 ctgcgcggcg acgacggcaa cgatcaactg gtcggcgggg ccggcaacga cgtcatgctc
     1561 ggcggccgcg gcgtcgacgg gttcgacggc ggcggcaacg aacccttcgg cccctcgagc
     1621 agcttcggcg accaggtcag cttcctcgat tcggccgcga cccagggcgc cgtggcggac
     1681 ctccacacgg ggatcatctc gaacgacggc ttcggaaatg cggagtcgat gaccggcatc
     1741 gagtcgcttc gcggcaccgg gctgggcgac ttcctcggcg gggacgccgc ggccaaccgc
     1801 ctcgtcggcg agggcgggga ggacgtcatc ttcggccgcg gcggcgacga tatcatcgag
     1861 ggcggcggcg gggtcgatct cctccacggc catgaaggcg acgacagcct gtcgggcggc
     1921 agcggcaacg acctgctgcg cggctatgcc
//
LOCUS       DASVQZ010000172         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_614202, whole genome shotgun sequence.
ACCESSION   DASVQZ010000172 DASVQZ010000000
VERSION     DASVQZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614202"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2298
                     /gene="pnp"
                     /locus_tag="VF547_04815"
     CDS             <1..2298
                     /gene="pnp"
                     /locus_tag="VF547_04815"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX8262176.1"
                     /translation="VKPGQDFFPLTVHYQEKFSAAGRIPGGFFKRERGATEKETLTSR
                     LIDRPIRPLFPEGFYNEVLCIAHVMSYDGENEPDVLAMIAASAALTLSGIPFMGPIAA
                     ARVGYAEGDYILNPSQTQIQEGELDLVVAGTHNAVMMVESEAKELSEDVMLGAVMFAH
                     RSIQPVIKAIISLAEKAAKDPWELEEAADTKAVKQRLRDLIGDDLAAAYRLTDKQARQ
                     TAVNEARAKAREGLADMEASDPAGYLGALKLVKKLEAEIVRGAILKDGVRIDGRDTRT
                     VRPIEAMVGFLPRTHGSALFTRGETQAICTTTLGTKDAEQMIDGLEGLSYSRFMLHYN
                     FPPYSVGEVGRFGAPGRREVGHGKLAWRALHPVLPSHDEFPYTIRVLSDITESNGSSS
                     MATVCGGCLALMDAGVPIARPVSGIAMGLIKEGDDFAVISDILGDEDHLGDMDFKVAG
                     TEKGITSLQMDIKIAGITEEIMRIALDQANEGRAHILKEMGKALGDVRGELSAHAPRI
                     ETMQIDKAKIRDVIGTGGKIIREIVATTGAKVDIDDEGVIKISSSDVNQIDAARKWIQ
                     GIVEEPEVGKVYSGKVVNLVDFGAFVNFMGSRDGLVHVSEIRNERVERVADALPEGQE
                     VKVKVLEIDPRGKVRLSMRVVDQETGAELEDTRPPREGREDRPRSDRGDRGDRGGRDR
                     GPRRDGDRGGRDRGPRREGGGDRDRGPRGEGGGDRGPRGEGGGERGDRGDRGPRRDRE
                     DRVPRDNDGGPEPEFAPAFLTRDDD"
BASE COUNT          424 a          871 c          836 g          317 t
ORIGIN      
        1 gtcaagccgg ggcaggattt cttcccgctg accgtgcact atcaggaaaa attctcggcc
       61 gccgggcgca tcccgggcgg cttcttcaag cgcgagcgcg gcgccaccga gaaggagact
      121 ctcaccagcc gcctcatcga ccggccgatc cggccgttgt tcccggaggg cttctacaac
      181 gaggtgctct gcatcgccca cgtcatgagc tatgacggcg agaacgagcc ggacgtgctg
      241 gcgatgatcg ccgcctccgc tgccctcacc ctcagcggga tcccgttcat gggcccgatc
      301 gccgcggccc gggtcggcta tgccgagggc gactatatcc tcaacccgtc ccagacccag
      361 atccaggaag gcgaattgga cctcgtcgtc gccggcaccc acaacgccgt gatgatggtc
      421 gaatcggagg ccaaggagct ttccgaggac gtcatgctcg gcgccgtcat gttcgcgcac
      481 cgctcgatcc agccggtgat caaggcgatc atcagcctcg ccgagaaggc cgccaaggac
      541 ccgtgggagc tcgaggaggc cgccgacacc aaggcggtca agcagcgcct ccgcgacctg
      601 atcggcgacg acctcgccgc cgcctatcgc ctcaccgaca agcaggcccg ccagaccgcg
      661 gtcaacgaag cccgcgccaa ggcccgcgag ggcctcgccg acatggaggc cagcgacccc
      721 gccggctatc tgggagcctt gaagctcgtc aagaagctcg aggccgagat cgtccgcggc
      781 gccatcctca aggacggcgt ccgcatcgac ggccgcgaca cccgcaccgt ccggccgatc
      841 gaggcgatgg tcggcttcct cccgcgcacc cacggctcgg ccttgttcac ccgcggcgag
      901 acccaggcga tctgcacgac cacgctcggc accaaggacg ccgagcagat gatcgacggg
      961 ctggagggcc tctcctactc gcgcttcatg ctccactaca acttcccgcc ctactcggtc
     1021 ggcgaggtcg ggcgcttcgg cgctccgggg cggcgcgagg tcggccacgg caagctcgcc
     1081 tggcgggcgc ttcacccggt gctgcccagc cacgacgaat tcccctacac gatccgggtc
     1141 ctctccgaca tcacggagag caatggctcc tcgtcgatgg cgacggtgtg cggcggctgc
     1201 ctcgcgctga tggacgccgg agtcccgatc gcgcggccgg tctcgggcat cgccatgggc
     1261 ctgatcaagg agggcgacga cttcgccgtc atctccgaca tcctcggcga cgaggaccat
     1321 ctcggcgaca tggacttcaa ggtcgccggc acggagaagg gcatcacctc gctccagatg
     1381 gacatcaaga tcgccggcat caccgaggag atcatgcgca tcgccctcga ccaggccaat
     1441 gagggccggg cccatatcct caaggagatg ggcaaggcgc tcggcgacgt ccgcggcgag
     1501 ctgtcggcgc acgcgccgag gatcgagacg atgcagatcg acaaggccaa gatccgcgac
     1561 gtcatcggca cgggcggcaa gatcatccgc gagatcgtcg ccaccaccgg cgccaaggtc
     1621 gacatcgacg acgagggggt gatcaagatc agctcctccg acgtcaacca gatcgacgcg
     1681 gcgaggaagt ggatccaggg catcgtcgag gagcccgagg tcggcaaggt ctattccggc
     1741 aaggtcgtca acctcgtcga tttcggcgcc ttcgtgaact tcatgggcag ccgcgacggc
     1801 ctcgtccacg tctccgagat ccgcaacgag cgggtggagc gggtcgccga cgcgctgccg
     1861 gagggccagg aggtcaaggt caaggtcctc gagatcgatc cgcgcggcaa ggtgcggctg
     1921 tcgatgcggg tcgtcgacca ggagaccggc gccgagctcg aggacacgcg tccgccgcgc
     1981 gagggccgcg aggaccgtcc gaggagcgac cgcggcgatc gcggtgaccg gggcggccgc
     2041 gaccgcggcc cacgccgcga cggcgaccgc ggcggccgcg accgcggccc ccgccgcgaa
     2101 ggcggcggcg accgcgaccg gggtccgcgc ggcgagggcg gcggcgaccg cggcccgcgc
     2161 ggcgaaggcg gcggcgagcg gggcgaccgc ggcgaccggg gtccccgccg ggaccgcgag
     2221 gaccgcgtcc cgcgcgacaa tgacggcggc ccggagccgg aattcgcccc ggccttcctg
     2281 acccgcgacg acgactgatc ctttcctccc catccgcaag gatggggagg cggaccgcca
     2341 attttggcgg tggaggggga gctggctcgg tttcggaggt ccccgcagga accgccggcc
     2401 cggccttccc ttctggaaca gacagaaagg cccccgcaga aatgcggg
//
LOCUS       DASVQZ010000173         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_423155, whole genome shotgun sequence.
ACCESSION   DASVQZ010000173 DASVQZ010000000
VERSION     DASVQZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423155"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF547_04820"
     CDS             <1..404
                     /locus_tag="VF547_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX8262177.1"
                     /translation="LADATTGRLLPPLTAADARTTVESRYTGKAKVKAIDRTSADDPP
                     LDLRRKVAAWRVTMSDGTRFYLDAATGEILARRTSWWRFYDFMWGLHIMDLETREDAH
                     NPLLIGFALVSLATVLLALALLPLALKRRRR"
     gene            401..1024
                     /locus_tag="VF547_04825"
     CDS             401..1024
                     /locus_tag="VF547_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX8262178.1"
                     /translation="MTGPSALLGSALPYLDWAGVAVFAFSGALVAAAKRLTLVTFIFF
                     AVITGIGGGTARDLLIGAPVFWVHENAILLICFAAALAVWFSPGTLWKGRALPWLDAA
                     GIAAYATYGAAKGLAFGLAPLPALAMGVLTACLGGIVRDVLAGEPSILIRPELYVTAA
                     ALSAALMVGMLLAGIPAAPAGLVAAAAGFALRAGAIHYGWKLPAYPR"
     gene            1068..1670
                     /locus_tag="VF547_04830"
     CDS             1068..1670
                     /locus_tag="VF547_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8262179.1"
                     /translation="MGGKDTRGRIVEAAMELFWLKGYGSTSIADILSRSQVNSGSLYH
                     FFPGKQDLLIAVLETYRDGIGPMLLDPAWAGVADPVEKVFALLAKYRSLIVETECGYG
                     CPIGSLALELHEADPAVRRLLAENFERWVEAVAACLEQARHRFPPAADLRALAELALT
                     VMEGGVMQARTHRDVGHFDRSVAQLRAYFETLMVKEGEAA"
     gene            1667..2635
                     /locus_tag="VF547_04835"
     CDS             1667..2635
                     /locus_tag="VF547_04835"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8262180.1"
                     /translation="MKTLIAALALGLAAAPPSLAATTARAPDGTRLLSHELVIDAPPE
                     QVWEAVATAEGWKRWAVPVAWSPAPDLIETSYTPTARPGDPSTIRQQVLLRLPERLMV
                     FRTVKAPAGFPDFDTFARVVSVFELEPAGEGRTRVRLTGSGYAETEAGRRLLAFFERG
                     NAVSLEALRAHFAKSAAAPAPLRAELEPLRFLLGHCWRGEFRDGAVDTHCFESVYGGL
                     HVRDRHEVKGPGAPYRGETLYSWNAESKQVEYTYWNSSGGVSRGTMAAKPDALDFGNE
                     TYVGADGRRVSIATMWRKAGAQAYEAVSTSGADPTGSRVVRYVRID"
     gene            complement(2897..>3132)
                     /locus_tag="VF547_04840"
     CDS             complement(2897..>3132)
                     /locus_tag="VF547_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262181.1"
                     /translation="TQAIAAITLPNPASVGLHERLGFAPAGVYRQVGWKLGRWHDVGL
                     WQRPLAAAGMPPPEPRPPRLADFLQSVIGPDGS"
BASE COUNT          430 a         1121 c         1074 g          507 t
ORIGIN      
        1 gccttgccga cgctaccacc ggaaggctgc tgccgccgct caccgccgcc gatgcccgga
       61 cgacggtcga gagccgctac accggcaagg cgaaggtgaa agcgatcgat cgcacatcgg
      121 ccgacgatcc gccgctcgac ctgcgccgca aggtcgccgc ctggcgggtg acgatgagcg
      181 acggcacccg cttctacctc gacgcggcca ccggcgagat cctggcgagg cgaacctcct
      241 ggtggcgctt ctacgatttc atgtggggcc tccacatcat ggacctcgag acccgcgagg
      301 acgcccacaa tccgctcctg atcggcttcg ccctcgtctc gctggcgacc gtgctgctcg
      361 cccttgccct gcttccgctg gccctcaagc gccgccgccg gtgaccggcc cttccgcctt
      421 gctgggctcg gctttgccct atctggactg ggcgggagtg gcggtcttcg ccttttccgg
      481 cgcgctggtc gcggcggcga agcggctgac gctcgtcacc ttcatcttct tcgccgtgat
      541 caccggcatc ggcggcggca ccgcgcgcga cctgctcatc ggcgctccgg tcttctgggt
      601 gcacgagaac gcgatcctcc tcatctgctt cgcggcggcg ctcgcggtgt ggttctcccc
      661 cggcaccttg tggaaggggc gggcgctccc ctggctcgac gcggccggaa tcgcggccta
      721 cgccacctac ggcgccgcca agggcctcgc cttcggcctc gcgccgctcc cggcgctggc
      781 gatgggcgtt ctgaccgcct gcctcggcgg aatcgtccgc gacgtcctcg ccggcgagcc
      841 ctccatcctg atccggcccg agctctacgt caccgccgcc gctctctcgg cggcgctgat
      901 ggtcgggatg ctccttgccg gcatcccggc ggcgccggcc gggctggtcg ccgcggcggc
      961 cggcttcgcg ctgcgcgccg gcgcgatcca ttacggctgg aagctccccg cctacccacg
     1021 ctgatttcct gttgacttga ttgaacgttc aatcaagaat gcggcccatg ggcggcaagg
     1081 atacgcgcgg gcggatcgtc gaggcggcga tggagctctt ctggctgaag ggctacggct
     1141 cgacctcgat cgccgacatc ctgagccgga gccaggtcaa cagcggcagc ctctatcatt
     1201 tctttcccgg caagcaggac ctgctgatcg ccgtgctcga aacctatcgc gacggcatcg
     1261 gcccgatgct gctcgatccg gcctgggccg gagtggccga tccggtcgag aaggtcttcg
     1321 ccctcctcgc caaataccgc tcgctgatcg tcgagaccga gtgcggatat ggctgcccga
     1381 tcggcagcct cgcgctcgag ctgcacgagg cggatccggc cgtgcgccgg ctgctcgccg
     1441 agaatttcga gcgctgggtc gaggcggtgg cggcctgcct ggagcaggcg cggcaccgtt
     1501 tcccgccggc tgcggacctg cgggcgctgg ccgagctcgc gctcaccgtc atggagggcg
     1561 gcgtgatgca ggcccgaacc caccgcgacg tcggccattt cgaccggtcg gtggcgcaat
     1621 tgcgcgccta tttcgaaacc ctgatggtga aggaagggga agcggcatga agaccttgat
     1681 cgcggccttg gccctgggcc tcgcggcggc cccgccgtcg ctcgcggcga cgacggcgcg
     1741 cgcgcccgac ggaacccgcc tgctgtcgca cgagctggtg atcgacgccc cgccggagca
     1801 ggtatgggag gcggtcgcga ccgcggaggg ctggaagcgc tgggcggtcc ccgtcgcctg
     1861 gtccccggcg cccgacctga tcgagacgag ctacacgccg accgcccggc ccggcgaccc
     1921 ttctaccatc cgccagcagg tgctgctccg gctgccggag cggctgatgg tcttccgcac
     1981 cgtcaaggcg ccggcgggct ttccggactt cgacaccttc gccagggtgg tgagcgtttt
     2041 cgagctggag ccggccgggg aggggcggac ccgggtccgg ctcaccggct cgggctatgc
     2101 ggagacggag gcgggccgcc ggctgctcgc tttcttcgag cgcggcaatg ccgtctcgct
     2161 cgaggcgctg cgcgcgcatt tcgcgaaatc ggcggccgct ccagccccgc ttcgcgccga
     2221 gctcgagccg ctgcgcttcc tgctcggcca ttgctggcgc ggcgaattcc gggacggcgc
     2281 cgtcgacacc cattgcttcg agagcgtcta cggcggcctg cacgtccgcg accggcacga
     2341 ggtgaagggc cccggcgcgc cttaccgggg cgagacgctc tacagctgga acgccgaatc
     2401 gaagcaggtc gaatatacct attggaactc ttcgggcggc gtcagccgcg gcaccatggc
     2461 ggcgaagcca gacgcgctcg acttcggcaa cgagacctat gtcggcgccg acgggcgcag
     2521 ggtctcgatc gcgacgatgt ggcgcaaggc gggcgcccag gcctatgagg ccgtcagcac
     2581 ctcgggcgcc gatccgaccg gcagccgcgt cgtccgctac gtccggatcg actagagcct
     2641 cggccgtcgg atcgcgctct cttccgttcg ggctgagctt gtcgaagccc ctctcctttt
     2701 ctctcatggc acgacaagca aagcccttcg acgggctcag ggcgaacggg tgagagcgat
     2761 tcaatgggag cgtgatcgcc ttccaccgaa ccgctcatcc tgagtcggga ctgagcgaag
     2821 gcgaaggccc gaatcgaaag accgcccttc aatacgccgg ttcgactacg ctcaccggtt
     2881 cctcaggatg agcgacctag gatccatctg gcccgatcac gctctggagg aagtcggcga
     2941 ggcgcggcgg ccggggctcg ggcggcggca tgccggccgc cgccagcggg cgctgccaca
     3001 ggccgacgtc gtgccaccgg ccgagcttcc agccgacctg gcggtagacg cccgcggggg
     3061 cgaagccgag ccgttcgtgg aggccgacgc tggccgggtt cggcagggtt atcgccgcaa
     3121 tcgcctgggt ga
//
LOCUS       DASVQZ010000174         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_712816, whole genome shotgun sequence.
ACCESSION   DASVQZ010000174 DASVQZ010000000
VERSION     DASVQZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712816"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..95
                     /locus_tag="VF547_04845"
     tRNA            19..95
                     /locus_tag="VF547_04845"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Asp,seq:gtc)
     gene            138..905
                     /locus_tag="VF547_04850"
     CDS             138..905
                     /locus_tag="VF547_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8262182.1"
                     /translation="MASRYDRIGLNYAQLRKPDPRIAAAIDGALGGARSVLNVGAGAG
                     SYEPAGREVTALDPSIEMIRQRSAPAAAVVQGRAEELPFGDDSFDAAMAALTVHHWSD
                     RPEGLREIRRVTRGPVVILAYDPGFRGFWLTDYLPELIGLDEAQMPRLTEYEKWLGPV
                     TIVPVPIPHDCTDGFLCAYWRRPAAYLDPRARAAMSSFWALGDVSPALARLEADLESG
                     AWAERYGGLLALDEHDCGYRLVVAGPDGTAGSRKGAR"
     gene            complement(1075..1299)
                     /locus_tag="VF547_04855"
     CDS             complement(1075..1299)
                     /locus_tag="VF547_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEX8262183.1"
                     /translation="MPLEPARPSWRKPAGIFLILLLIAAWGFAVVTLAEALPSLPWPL
                     KALFFIAAGLVWIVPLKPLLRWMELGRWRE"
     gene            complement(1290..1874)
                     /locus_tag="VF547_04860"
     CDS             complement(1290..1874)
                     /locus_tag="VF547_04860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX8262184.1"
                     /translation="MVVPSPSPPGKARLRAEALERRRAYARSLAPGMRAELERKLARI
                     VLPRLIGARVVAGYHPLKDEVSPYPILERLGPGQRAVLPWFLDRDARMLFREAPATEP
                     SPWGVLQPPAQAEALAPDVVLVPLVAADRSGSRIGHGKGHYDRALSHLREGGAVLTIG
                     LGWEAQILDDPIPADPWDVPLDAVATPLEWIECR"
     gene            complement(2025..>2319)
                     /locus_tag="VF547_04865"
     CDS             complement(2025..>2319)
                     /locus_tag="VF547_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX8262185.1"
                     /translation="RKYSVACRDGEEEHLRSVAAIVDSKAQDAAAALGSLSESRQLLF
                     ASLLLADELKDHRSGRPPAAPAEPDPSLADALERLAERVESLAEQLERSAPAS"
BASE COUNT          330 a          828 c          837 g          324 t
ORIGIN      
        1 tagttcgccg gctcccacgc gggtgtagct cagttggtta gagcgccggc ctgtcacgcc
       61 ggaggtcgcg ggttcgagcc ccgtcactcg cgccatttcc cattcccccg ccgccgcccc
      121 gcggccagag acgcgcgatg gcgagccgct acgaccgcat cgggctgaac tatgcccagc
      181 tgaggaagcc cgatccgagg atcgcggcgg cgatcgacgg cgccctcggc ggggcccgga
      241 gcgtcctcaa cgtcggcgcc ggcgccggtt cgtacgagcc tgccggccgg gaggtgaccg
      301 ccctcgatcc gtcgatcgag atgatccgcc agcgctcggc gcccgcggcc gcggtcgtcc
      361 aggggcgggc ggaggagctg ccgttcggcg acgacagctt cgacgccgcg atggcggccc
      421 tcaccgtcca ccattggtcc gaccggcccg aaggccttcg cgagattcgg cgggtgaccc
      481 gcggcccggt cgtcatcctc gcctacgatc ccggcttccg cggcttctgg ctcacggatt
      541 acctgccgga gctgatcggc ctcgacgagg cccagatgcc gcggttgacc gaatatgaga
      601 aatggctcgg tccggtcacg atcgttcccg tgccgatccc gcacgactgc accgacggct
      661 tcctgtgcgc ctactggcgg cgcccggccg cctatctcga cccgagggcg cgagcggcca
      721 tgtcgtcctt ctgggcgctc ggcgacgtct ccccggcgct ggccaggctc gaggccgacc
      781 tcgaaagcgg cgcctgggcc gagcgttatg gcgggctcct cgccctcgac gagcacgact
      841 gcggctaccg cctcgtcgtc gccggcccgg atggcaccgc aggaagccga aagggggccc
      901 gttgagctgg agcgtccgcc agtcggatga ccccgcctcc gttcgggctg agcttgtcga
      961 agcccttctc gtccccctgg caaagccgga agaaagccct tcgacaggct cagggcgaac
     1021 ggattgaggc ggcccgatcc gactcgtcga tgctctggcg gtcggctttc gccttcactc
     1081 gcgccaccgg cccagctcca tccaccgcag cagcggcttg agcggaacga tccagacgag
     1141 gccggcggcg atgaagaaca gggccttcag cggccagggg agggacggga gcgcctcggc
     1201 gagggtcacc accgcgaacc cccatgccgc gatcagcagc aggatcagga agatccccgc
     1261 cggcttgcgc cagctcggcc tggcgggctc tagcggcatt cgatccactc gagcggcgtg
     1321 gcgacggcgt cgagcgggac gtcccacggg tcggcgggga tcgggtcgtc gaggatctgc
     1381 gcttcccagc ccagcccgat cgtgagcacc gcgccgcctt cgcgaaggtg cgacagcgcc
     1441 cggtcgtaat ggcccttgcc atggccgatc cggcttcccg accggtcggc cgccaccagc
     1501 gggacgagca cgacgtccgg cgccagggcc tcggcttgcg ccggcggctg cagcacgccc
     1561 caggggctgg gctcggtcgc gggcgcctcg cggaacagca tccgggcgtc gcggtcgagg
     1621 aaccagggga ggacggcgcg ctgcccgggg ccgagccgct ccaggatcgg ataggggctg
     1681 acctcgtcct tcagcgggtg atagccggcg acgacccgcg ctccgatcag ccgcgggagg
     1741 acgatccggg cgagcttgcg ctccagctcc gcgcgcatcc cgggggccag ggagcgggca
     1801 taggcgcgcc gccgctcgag cgcctcggcg cggagccggg ccttgccggg aggggagggt
     1861 gacgggacca ccatggccgt gtgcttgaat atcctctgac gcgtcaacgt caggtgggga
     1921 ccatatgtgc cggaccaggg cccggccagg gacagccccc gaggatgctt atcgcccagg
     1981 gatgttcaaa aagcgcgcac cgggcagtac ccgtcagcct ctatctagga agccggggcg
     2041 ctccgctcaa gctgttcggc gaggctttcg acccgctcgg cgaggcgctc cagcgcatcg
     2101 gccagggacg ggtcgggctc ggcgggcgcc gccggcggcc ggccggagcg atggtccttg
     2161 agctcgtcgg cgagcagcag cgaggcgaac agcagctgcc tcgactcgct gaggctgccg
     2221 agggcggcgg cggcgtcctg ggccttggaa tcgacgatcg cggcgaccga gcgcagatgc
     2281 tcctcctcgc cgtcgcggca ggcgaccgaa tatttgcgg
//
LOCUS       DASVQZ010000175         4041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_808091, whole genome shotgun sequence.
ACCESSION   DASVQZ010000175 DASVQZ010000000
VERSION     DASVQZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808091"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VF547_04870"
     CDS             complement(<1..513)
                     /locus_tag="VF547_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8262186.1"
                     /translation="MTRSAARAAAGTAPSAPPLRLMIVDDSQVARAVLTRMLAGFADF
                     EIVATAADAGEALRLLPGTKVDIVLLDVEMPGESGLEALPEIVRLGGGARVLIVSSFC
                     DGAAEAAVEALGPGAAETLPKPGAGSFGGRFSEVLADRLRRMARPEPAAPAAAAPPEA
                     KLGCLAVGAST"
     gene            complement(510..875)
                     /locus_tag="VF547_04875"
     CDS             complement(510..875)
                     /locus_tag="VF547_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8262187.1"
                     /translation="MKSCLVVDDSKVIRKVARHILETLHFSVDEAEDGREALSRCEAR
                     MPDVVLLDWNMPVMGGMEFLRTLRQKKFETQPKVVFCTTENDAAHIRAAIDAGADEYV
                     MKPFDRETLESKLQIVGVA"
     gene            complement(943..1377)
                     /locus_tag="VF547_04880"
     CDS             complement(943..1377)
                     /locus_tag="VF547_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX8262188.1"
                     /translation="MAELLLIVRLAGERIAFRAADVESVVEIDSLTPVPGAARHVAGL
                     AALRSRVLTVIDCLASLDPGRSLCEGRFEAVMTVVDGHPYALMVEAVEDVVESTGEPR
                     PAMASLAGGWRRIARATVEAEGDLLLLADVEALIAGPARAAA"
     gene            complement(1370..3718)
                     /locus_tag="VF547_04885"
     CDS             complement(1370..3718)
                     /locus_tag="VF547_04885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX8262189.1"
                     /translation="MDDLINDFIAETREMLEALSGEIVAWETTPDDRGRLDEIFRFVH
                     TVKGNSGFFDLPRIRALSHAAEDALAAVRSGERRADAPLVSAVLAVIDRIGELVEALE
                     TGEALASEDDSALIDALSCDSAAEAPDVESAPAGPAARAPSRTIRLPVDLLDRMMSGV
                     SDLVLARNELARRIRANGGTETQAAFDRVSATIADLRDSITRTRMQRIDNLFAPLPRM
                     VRDLAGRTGKQMRLEIDGGDVEIDREMIEMIRDPLSHIVRNAIDHGIESPAERRRVGK
                     GEEGCLRVHARQAGNQILLEISDDGRGVDGDKLVRRAVAAGLLSAERAERLDETQKAA
                     LIFEAGLSTAATVTDLSGRGVGMDVVRANIERIGGLVEVASRKGQGVALTLRVPLTLT
                     IIPALTVAAAGQVYAIPRSAIEEIVRVRGEGVRVESVGGAAVATIRGRRLPLVHLRSA
                     LGCDPAADPAAQSLVLLKPAGADLYALAVDDIHDHEELVVKPAAPAVMAAGLYAGTTI
                     ADDGKPVLLLDPPGIAARAGLALHHESGAVEAAPAPAAAAAPEGPPALLFSTLAGARR
                     LVPVAAVQRIEDVRPEAVRLSAGRLHVSLGDALLPLEGCGGEVPAGPIRLLRLGDGIA
                     EIAYGFAEVVDIIPIPAGMLAAAEPGEVRGVVQVGSGQAELLDLHWLFARHAGRSAAG
                     AARPVCAIPAGDPFMDAILRPLVESAGYTVVASGAPGSESARIVIAAAEAGDPPAPEG
                     ARVVRVRASAEGGDDSVHRYDRDALLAALAGEAPARRRRRRG"
BASE COUNT          539 a         1457 c         1511 g          534 t
ORIGIN      
        1 ggtcgacgct ccgaccgcga ggcagccgag cttcgcctcg ggcggcgcgg cggcggccgg
       61 cgccgccggc tcggggcgcg ccatgcgcct cagccggtcg gcgagcacct ccgagaagcg
      121 gccgccgaag ctcccggcgc ccggcttggg aagggtctcc gccgccccgg gcccgagcgc
      181 ctcgaccgcc gcctcggcgg cgccgtcgca gaaggaggag acgatcagca cccgggcgcc
      241 gccgccgagc ctgacgatct cgggaagcgc ctcgagcccg ctctcgccgg gcatctcgac
      301 gtcgagcagg acgatgtcga ccttggtccc gggcagcagc cggagcgcct cgccggcgtc
      361 ggcggcggtg gcgacgatct cgaaatcggc gaagccggcg agcatccgcg tgagcaccgc
      421 cctcgcgacc tgcgaatcgt cgacgatcat cagcctcagg ggcggcgcgg acggagcggt
      481 gcccgccgcc gcgcgggcgg cggagcgggt cacgcgaccc cgacgatctg cagcttgctc
      541 tcgagcgtct cgcggtcgaa cggcttcatc acatattcgt cggcgccggc gtcgatcgcg
      601 gcgcggatgt gggcggcgtc gttctcggtc gtgcagaaga cgaccttggg ctgggtctcg
      661 aacttcttct ggcgaagagt cctcaggaac tccatcccgc ccatcaccgg catgttccag
      721 tcgagcagga cgacgtcggg catgcgggcc tcgcagcgcg acagcgcctc gcggccgtcc
      781 tccgcctcgt cgacggagaa atggagcgtc tccaggatat ggcgggcgac cttccggatc
      841 accttcgaat cgtcgacgac aagacagctc ttcatgctcg ctctcgctct ttccggcggc
      901 gccatcctag ccgcaattcg ttaatcagcg gcttaaggcc gctcatgccg ccgcccgggc
      961 cgggccggcg atcagcgcct cgacgtcggc gagcaggagc aggtccccct cggcctcgac
     1021 cgtggcgcgg gcgatccggc gccagccgcc cgccagcgag gccatggccg gccggggctc
     1081 gccggtcgac tcgaccacgt cctccaccgc ttcgaccatc aaggcgtagg gatggccgtc
     1141 caccaccgtc atcaccgctt cgaagcggcc ttcgcagagg cttcggccgg gatcgagcga
     1201 ggcgaggcag tcgatcaccg tcagcacccg gctgcgcagc gcggcgagcc cggcgacatg
     1261 gcgagcggcg cccggcaccg gggtcagcga atcgatctcg acgaccgatt cgacgtcggc
     1321 ggcgcggaag gcgatccgct cgccggcgag gcggacgatg aggaggagct cagccacgac
     1381 ggcgcctcct cctcgcggga gcctcgcccg ccaaagcggc gagcagggcg tcgcggtcgt
     1441 agcgatggac gctgtcgtcg ccgccctcgg cggaggcgcg cacccggacg acgcgggcgc
     1501 cttcgggcgc gggcgggtcg ccggcctcgg cggcggcgat gacgatccgg gccgactcgc
     1561 ttccgggagc gccgctggcc accaccgtgt agccggcgct ctcgacgagc gggcgcagga
     1621 tcgcgtccat gaacggatcg cccgccggaa tggcgcagac cggccgagcg gcgccggcgg
     1681 ccgagcggcc ggcgtggcgg gcgaacagcc agtgcaggtc gagcagctcg gcctggccgg
     1741 acccgacctg gacgactccg cgcacctcgc cgggctcggc cgcggcgagc atcccggccg
     1801 ggatcgggat gatgtcgacc acctcggcga agccgtaggc gatctcggcg atgccgtcgc
     1861 cgaggcggag caggcggatg gggccggctg ggacctcgcc gccgcagcct tcgagcggca
     1921 gcagggcgtc gccaagggag acgtggaggc ggccggcgct gagccggacc gcttcgggcc
     1981 ggacgtcctc gatccgctgc acggcggcga ccggaaccag cctgcgggcg ccggcgaggg
     2041 tgctgaacag cagggccggc gggccttcgg gagccgccgc tgcggcgggc gcgggcgccg
     2101 cctcgaccgc gcccgattcg tggtgcagcg ccagcccggc gcgggcggcg atgcccggcg
     2161 ggtcgagcag cagcacgggc ttgccgtcgt cggcgatcgt ggttccggca tagaggccgg
     2221 cggccatcac cgccggggcg gcgggcttga ccaccagctc ctcgtgatcg tggatgtcgt
     2281 ccacggcgag cgcgtagagg tcggcgccgg ccggcttgag caggaccagg ctctgcgccg
     2341 ccgggtcggc cgcgggatcg cagcccagcg ccgagcgcag atggaccagc ggcaggcgcc
     2401 ggccgcggat ggtggcgacg gcggcgccgc cgacgctctc cactcggact ccttcgcccc
     2461 ggacccggac gatctcctcg atggccgagc gcgggatggc gtagacctgg ccggcggcgg
     2521 cgacggtgag ggcgggaatg atggtgaggg tgagcgggac gcgcagggtc agggcgaccc
     2581 cctgcccttt ccggctggcg acctcgacga ggccgccgat ccgctcgata ttggcccgga
     2641 ccacgtccat cccgacgccg cgcccggaca ggtcggtcac cgtcgcggcg gtcgacaggc
     2701 cggcctcgaa gatcagggcc gccttctggg tctcgtcgag ccgctcggcg cgctcggcgc
     2761 tcagcagccc ggcggcgacg gcccggcgga ccagcttgtc gccgtcgacg ccccggccat
     2821 cgtccgagat ctcgagcagg atctggttgc cggcctggcg ggcatggacc cggaggcagc
     2881 cctcctcgcc cttcccgacc cggcgccgct cggccgggct ctcgatgccg tggtcgatgg
     2941 cgttgcggac gatatggctg agcggatcgc ggatcatctc gatcatctcg cggtcgatct
     3001 cgacgtcgcc gccgtcgatc tcgagccgca tctgcttgcc ggtgcggccg gcgaggtcgc
     3061 ggaccatccg cggcagcggc gcgaacagat tgtcgatccg ctgcatccgg gtgcgggtga
     3121 tcgagtcgcg aaggtcggcg atcgtcgccg agacccggtc gaaggcggcc tgagtctcgg
     3181 tgccgccgtt cgcgcggatc cggcgggcga gctcgttgcg ggccagcacc aggtcggaaa
     3241 cgccgctcat catccggtcg agtaggtcga cgggcaggcg gatggtccgg ctgggcgccc
     3301 gggcggccgg gccggccgga gcggactcga cgtccggcgc ctcggcggcc gagtcgcagg
     3361 acagggcatc gatcagggcg ctgtcgtcct cgctcgccag cgcctcgccg gtctcgagcg
     3421 cctcgaccag ctcgccgatc cggtcgatga ccgcgagcac ggcgctgacc agcggcgcgt
     3481 cggccctgcg ctcgccggaa cggaccgccg cgagcgcgtc ctcggcggcg tggctgaggg
     3541 cgcggatgcg cggcaggtcg aagaagccgc tgttgccctt gacggtgtgg acgaagcgga
     3601 agatctcgtc gaggcggcct cggtcgtcgg gcgtcgtctc ccaggcgacg atctcccccg
     3661 agagcgcctc cagcatttcc cgcgtctccg cgatgaagtc gttgatgaga tcgtccactg
     3721 gcccctgccg ctaacccgcg tctaacaagg cttaggcagg cttggttaag aaggcgttta
     3781 cttccctcca ccttgctccc ctccctgcga agggaggggt tgggggtggg taggcgagcg
     3841 cagcgagact ttctgccgag tccgagtttc ggccgccttc ggcggaccca cccccggccc
     3901 ctcccttcca gggagggagt caggccgccg gaatgctcgc cccgatcagg aggaccccct
     3961 cctcctggtc ggcgacctgg acctcgccgc cgcaatcggc gagcaggcag tggaccagcc
     4021 aggccgcggc gccgcgcggg g
//
LOCUS       DASVQZ010000176         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_518324, whole genome shotgun sequence.
ACCESSION   DASVQZ010000176 DASVQZ010000000
VERSION     DASVQZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518324"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..>2491)
                     /gene="pepN"
                     /locus_tag="VF547_04890"
     CDS             complement(63..>2491)
                     /gene="pepN"
                     /locus_tag="VF547_04890"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX8262190.1"
                     /translation="NGDHDRPLRLDSEGLNLIEVAVDGVPVEARIEGEKLTLPLTAGS
                     ASVETVVELAPRSNTQLMGLYESGGILCTQCEAEGFRRITPFPDRPDVLSRFRVRMIA
                     DKALYPVLLSNGDPVGAGELQDGRHWAEWDDPFPKPSYLFALVAGDLAANRDSFTTRS
                     GRRIDLGIWVREPDLAKTEHAMASLKAAMKWDEDVYGREYDLAVFNIVAVADFNFGAM
                     ENKGLNIFNSRFILADPETATDQDFDAVAGVVAHEYFHNWSGNRVTCRDWFQLSLKEG
                     FTVFRDQMFSADMGSAAVKRIEDVRLLRASQFPEDAGPLAHPVRPDSYLEIANFYTAT
                     VYNKGAELIRMLHTLLGPERFRAGTDLYFERHDGQAVTCEDFVRAMEEASGEDLTRFR
                     LWYEQAGTPRVRARLIRQDGTARLLLDQEVPPTPGQPAKRAMPIPLRVALFGEGGRKL
                     EERLVLHDEPHSEAEFAAAGDRLVLSINRGFSAPVVVEAQRSAADLAFLSAHDDDPFA
                     RYEATQQLMLDTLLAAIGGTVEHRAVVEAVGRTLGDQALDPAFIAEAVLLPSESFIGD
                     QMAVVDPEAIRAAREALRSELGRTLEAQWRAAYASTAANRYEYSPAAKGARRLRTVAL
                     NYLMAAGANDAAALARRQFDEADNMTDRQGALSALANSDAAERRGALDSFYERYRDDA
                     LVLDKWFTVQAVSTRDDALDQVVELARHPDFTLANPNRMRSLVAAFAANQRAFHDASG
                     RGYRFLADMILAVDRLNPQTAARLVPPLGRWRRFGERRQDLIKAELERIVATPGLSKD
                     VFEQASKSLA"
BASE COUNT          363 a          853 c          896 g          379 t
ORIGIN      
        1 ccggctttac ccgcgaagcg cgtccggccg ggtcgcccgc catcgacggc gcggcggcgc
       61 ggctaggcca ggctcttgct cgcctgctcg aacacgtcct tgctgaggcc gggcgtcgcc
      121 acgatccgct cgagctcggc cttgatcagg tcctggcgcc gttcgccgaa gcggcgccag
      181 cggccgagcg gcggcaccag ccgggcggcg gtctgcgggt tgagccggtc aaccgccagg
      241 atcatgtcgg cgaggaagcg gtatcctcgg cccgaagcgt cgtggaaggc gcgctggttg
      301 gccgcgaagg ccgcgaccag cgagcgcatc cggttcggat tggcgagggt gaaatcggga
      361 tgccgcgcca gctccaccac ctggtcgagg gcgtcgtcgc gagtcgagac ggcctgcacc
      421 gtgaaccatt tgtcgagcac cagagcgtcg tcccgatagc gctcgtagaa ggaatcgagc
      481 gcgccgcgcc gttcggctgc gtcggaattg gcgagggcgc tgagcgcccc ctggcggtcg
      541 gtcatgttgt cggcctcgtc gaattggcgc cgggccaaag cggcggcgtc gttcgccccc
      601 gccgccatca gatagttgag ggcgacggtc cggagccgcc gggcgccctt cgccgcgggc
      661 gaatattcgt agcggttggc ggcggtcgag gcgtaggcgg ctcgccactg ggcctccagc
      721 gtccgcccga gctcggagcg cagcgcctcg cgcgcggccc ggatcgcctc gggatcgacg
      781 accgccatct ggtcgccgat gaaggattcg ctcggcagaa gcacggcttc ggcgatgaac
      841 gccgggtcga gcgcctggtc gccgagcgtg cgtccgacgg cctcgacgac ggctcgatgc
      901 tcgacggtgc cgccgatcgc ggcgagcagg gtgtcgagca tcagctgctg ggtcgcctcg
      961 tagcgggcga acggatcgtc gtcatgggcc gacaggaagg cgaggtccgc cgccgagcgc
     1021 tgcgcctcga cgaccacggg ggccgaaaag ccgcggttga tcgacagcac gagcctgtcc
     1081 ccggcggcgg cgaactcggc ctcgctgtgc ggctcgtcgt gcaggacgag ccgctcctcg
     1141 agcttccgcc cgccctcgcc gaacagggcg acccgaagcg ggatcggcat ggcgcgcttg
     1201 gccggctggc ccggggtcgg cggcacctcc tggtcgagca gcaggcgcgc ggtcccgtcc
     1261 tgccggatca gcctcgccct gacccgcgga gtacccgcct gctcgtacca cagccggaag
     1321 cgggtcaggt cctccccgct tgcctcctcc atcgcccgga cgaaatcctc gcaggtcacc
     1381 gcctggccgt cgtggcgctc gaaatagagg tcggtcccgg cgcggaagcg ctcgggcccg
     1441 agcagggtgt ggagcatccg gatcagctcg gcgcccttgt tgtacaccgt cgcggtgtag
     1501 aaattggcga tctccagata gctgtccggc cgcaccggat gggcgagcgg gccggcgtcc
     1561 tcggggaatt gcgaggcccg cagcagccgc acgtcctcga tccgcttgac cgccgccgag
     1621 cccatgtcgg cggagaacat ctggtcgcgg aagaccgtga agccctcctt cagcgacagc
     1681 tggaaccagt cgcggcaggt gacccggttg cccgaccaat tgtggaaata ttcgtgcgcg
     1741 acgacgcccg ccaccgcgtc gaaatcctgg tcggtcgcgg tctccggatc ggcgaggatg
     1801 aagcgcgagt tgaagatgtt gaggcccttg ttctccatcg ccccgaagtt gaaatcggcc
     1861 acggcgacga tgttgaacac ggcgaggtcg tattcccgcc cgtagacgtc ctcgtcccat
     1921 ttcatcgccg ccttgagcga cgccatggca tgctcggtct tggccaggtc cggctcgcgc
     1981 acccagatgc cgaggtcgat gcgccggccc gagcgggtgg tgaagctgtc gcggttggcg
     2041 gcgaggtcgc cggcgaccag cgcgaacaaa tagctgggct tcgggaaggg atcgtcccat
     2101 tccgcccaat ggcggccgtc ctggagctcg ccggcgccga ccggatcgcc gttggagagg
     2161 agcaccggat agagcgcctt gtcggcgatc atccgcaccc ggaagcggct gagcacgtcc
     2221 ggccggtccg ggaagggcgt gatccgccga aagccctccg cctcgcactg ggtgcacagg
     2281 atgccgcccg attcgtacag gcccatcagc tgggtgttcg aacgcggggc gagctcgacc
     2341 acggtctcga ccgacgccga gccggcggtc aggggcaggg tcagcttctc gccctcgatc
     2401 cgcgcctcga ccgggactcc gtcgacggcg acctcgatca ggttcaggcc ctccgagtcg
     2461 agcctgaggg ggcggtcgtg gtcgccgttg c
//
LOCUS       DASVQZ010000177         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_423690, whole genome shotgun sequence.
ACCESSION   DASVQZ010000177 DASVQZ010000000
VERSION     DASVQZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423690"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..354
                     /locus_tag="VF547_04895"
     CDS             142..354
                     /locus_tag="VF547_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262191.1"
                     /translation="MTDRTRPDDSEIIDAMEDGPAFGDSSGGNLQRDIGSRDEIDEAI
                     GDGAGVTRVRDSDKPEQADLPRFNRK"
     gene            complement(542..1144)
                     /locus_tag="VF547_04900"
     CDS             complement(542..1144)
                     /locus_tag="VF547_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX8262192.1"
                     /translation="MSDARRSAPATERNRGPILEVLRDVLPERGLVLEVASGTGEHAV
                     FFAAALPRLLWQPSDPDPQAIASIAAWREQAGLANLLEPLRLDAADRSWPVARCDALL
                     CVNMAHISPWEATRGLMRGAGRLLPAGAPLALYGPFRRAGVPTAPSNEAFDESLKSRN
                     PQWGLRDLEAVAAEAALRGLRLAKVHEMPANNLTILFRRG"
     gene            complement(1141..1794)
                     /locus_tag="VF547_04905"
     CDS             complement(1141..1794)
                     /locus_tag="VF547_04905"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX8262193.1"
                     /translation="MTTSAGRLDNFTDAAFAFALSLLVIGRSQIPTDYAALSAAMADL
                     PAFAIGFAILGMFWYGHVRWRAYRGAGGAVSVLLTFVLVFLVLVYVQPLQAMSASLST
                     YLGGTGTRFTGDLAGMFAIYGAGFAGMAGVMAALFAEAERQPGASPSVRSGARGETVI
                     WLILAVTGTVSMLLSLIDSAAPFAPFVYSTLPLTVGLFAWRYPWGGAAADPEPAPEA"
     gene            complement(1831..3441)
                     /gene="gcvPB"
                     /locus_tag="VF547_04910"
     CDS             complement(1831..3441)
                     /gene="gcvPB"
                     /locus_tag="VF547_04910"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX8262194.1"
                     /translation="MSMNREGRPTRPESAEGAPPTHTGNRALMLEEPLLFEIGDSDTT
                     GVDFDQCSPAKAEARSGAETRPRPSPGSRLGGLERTRPIGLAGLTEPEAVRHYTRLSR
                     QNYAIDLGLFPLGSCTMKHNPRLCEKVARMPGFADIHPLQPVDTVQGAFEVIHRLGEW
                     LVRLTGMHSVAMSPKAGAHGELCGLLAIRSALEARGDAREVILVPESAHGTNPATAAF
                     AGYRVESIPATDRGRVDFEALQARLGPDVAAVMITNPNTCGLFEPEMTEISRAVHEAG
                     GLVYCDGANFNAIVGRVRPGDLGIDAMHINLHKTFSTPHGGGGPGSGPVVFSEALTPF
                     APLPFVEKQGDHFALVEEETAGEHHGSSFGRMVAFHGQMGMFTRALAYIMSHGADGLR
                     QVAEDAVLNANYVLRRLDDVLDAPFGASGPCMHEALFSDKGFAGGLTTLDLAKGLIDE
                     GYHPMTMYFPLVVHGAMLVEPTETESKAALDQFIGALRSVAERAKAGDQSLKSAPVHA
                     PRRRLDETLAARKPVLVWKEPDRAEAAE"
     gene            complement(3438..4796)
                     /gene="gcvPA"
                     /locus_tag="VF547_04915"
     CDS             complement(3438..4796)
                     /gene="gcvPA"
                     /locus_tag="VF547_04915"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX8262195.1"
                     /translation="MRYLPLADTDRQDMLRVVGASSIDDLFVDVPEAARLAAKIPGLP
                     DHLSEMAVERRLSALARRNLVAGDAPFFLGCGAYRHHIPASVDHLIQRGEFLTSYTPY
                     QPEIAQGTLQALFEFQTQVARLYGCDVANASMYDGSTACWEAIGMARRITRRGKAILS
                     SGLHPHYVSVARTMARFTGDLLETRIPELTGEPDTARLLDAIDGETSCVVVQYPDILG
                     RIGDLSELAERAHEKGALLIAVVTEPVALGLVRSPGEMGADIVVGEGQSIGVGLNFGG
                     PYVGLFATREKFVRQMPGRLAGETVDAEGKRGFVLTLSTREQHIRREKATSNICTSSV
                     LCALAFSIHLTLLGEKGLRQLAEANHRRALEAAGRLVALPGVELVNESFFNEFTLRLP
                     KEARPAVRAMADEGVLGGVSLGRLYPGADALENGLVVAVTETADRQDIEALVEALGEA
                     LA"
     gene            complement(4809..>5260)
                     /locus_tag="VF547_04920"
     CDS             complement(4809..>5260)
                     /locus_tag="VF547_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8262196.1"
                     /translation="LDPDAVASGVFAMIRPGGRFVGEMGGQGNIAALRAGIRAELSER
                     GYSVPAEDPQWYPGCEEFVRLYACAGFTEIQAQLIPRPTLLPAGVAAWVKTFRAGWLD
                     VAGVPEAHRDEVAAAVEARLQPQLRQPDGSWVADYVRLRFAMRKPAE"
BASE COUNT          823 a         1858 c         1863 g          716 t
ORIGIN      
        1 ctgcggtaca tcctcgacgc ccactggccg agcgacgtcc tcggcggctg ggcgttcggc
       61 gcggcctgga ccctgctcct ggtcaggctg ttcggaaccg cgcccccggc ccggcattga
      121 gtcctccgca aggagaggat catgaccgac cgtacccgcc ccgacgacag cgagatcatc
      181 gacgcgatgg aggacggccc cgccttcggc gactcatccg gaggcaacct ccagcgcgac
      241 atcggcagcc gcgacgagat cgacgaggcg atcggcgacg gcgccggggt cacccgggtc
      301 cgcgacagcg acaagcccga gcaggccgat ctccccagat tcaatcggaa gtgaggcaag
      361 tcgtcattgc gaggagcgca gcgacgcggc aatccagcgt gcgcccgagg ccgcaccgga
      421 ttgcttcgct tcgcccgcga tgacgattgg ccccttgagc gattagggtg aatcgctctc
      481 acccgttcgc cctgggcctg tcgaagggcc ggacggagaa agcgcgaccc gactgccggc
      541 gctagccccg ccggaacagg atcgtcagat tgttcgcggg catttcgtgc accttggcga
      601 ggcgcaggcc acgcaacgcc gcttccgccg ccacggcttc gaggtcgcgc agcccccatt
      661 gcgggttgcg cgacttcagc gactcgtcga acgcctcgtt gctgggcgcc gtcggaaccc
      721 cggcgcggcg aaagggcccg tagagggcca gcggggctcc ggcggggaga agccgcccgg
      781 cgccgcgcat cagccccctg gtggcctccc aggggctgat atgggccatg ttgacgcaca
      841 acagggcgtc gcagcgcgcc accggccagg aacggtccgc ggcatccagc cgcagcggct
      901 cgaggagatt ggcaagcccc gcctgctcgc gccatgcggc gatcgaggcg atcgcctgcg
      961 gatccgggtc cgagggctgc cagagcagcc gcggcaatgc cgccgcgaag aagacggcat
     1021 gctcccccgt cccgctcgcc acttcgagga ccagcccccg ctcgggaagc acgtcgcgaa
     1081 gcacctcgag gatcgggccg cggttccgct ccgtcgccgg ggccgagcgg cgggcgtcgc
     1141 tcacgcttcc ggcgccggct cgggatcggc ggccgcccct ccccagggat agcgccaggc
     1201 gaagaggccg acggtcagcg gcagggtcga gtagacgaac ggggcgaacg gcgcggcgga
     1261 gtcgatcagc gaaagcagca tcgagacggt gccggtgacg gcgaggatga gccagatgac
     1321 cgtctcgccg cgcgcgccgc ttcggaccga gggcgaggcg cccggctgcc gctccgcctc
     1381 ggcgaacagc gccgccatca cccccgccat gcccgcaaag ccggcgccgt agatggcgaa
     1441 catgccggcg aggtcgccgg tgaagcgggt gccggtgccg ccgagatagg tgctgagcga
     1501 ggccgacatc gcctgcagcg gctggacgta gaccaggacc aggaagacga ggacgaaggt
     1561 cagcagcacc gacacggcgc cgccggcgcc gcgatacgcc cgccagcgca catggccgta
     1621 ccagaacatg cccaggatcg cgaagccgat ggcgaaggcg ggaagatccg ccatcgccgc
     1681 gctgagcgcc gcgtagtcgg tcgggatctg gcttcgcccg atgaccagca acgacagggc
     1741 gaacgcgaac gccgcgtcgg tgaaattgtc gagccgcccc gccgacgtcg tcatcgtcac
     1801 cgggtagaac ggctccggaa ccagcgcaag ctactcggcc gcctccgccc ggtccggctc
     1861 cttccagacc agcaccggct tcctcgccgc cagggtctcg tcgagccgcc ggcgcggagc
     1921 gtggaccggg gcggacttca ggctctggtc gcccgccttg gcccgctccg ccaccgagcg
     1981 aagcgccccg atgaactggt cgagcgccgc cttgctctcg gtctcggtcg gctcgaccag
     2041 catcgcgcca tggacgacca gcgggaagta catggtcatc ggatgatagc cttcgtcgat
     2101 cagccccttg gcgaggtcga gcgtggtgag gccgccggcg aagcccttgt cgctgaacag
     2161 ggcctcgtgc atgcacggcc cgctcgcgcc gaacggcgcg tcgaggacgt cgtcgagccg
     2221 gcgaaggacg taattggcgt tcagcaccgc gtcctccgcc acctgcctca ggccgtcggc
     2281 gccgtggctc atgatgtagg cgagggcgcg ggtgaacatg cccatctggc cgtggaaggc
     2341 gaccatccgg ccgaagctgg agccgtgatg ctcgccggcc gtctcctcct cgaccagggc
     2401 gaaatgatcg ccctgcttct cgacgaacgg aagcggcgcg aacggggtca gagcctccga
     2461 gaagaccacc ggcccggagc ccgggccgcc gccgccgtgg ggcgtcgaga aggtcttgtg
     2521 gaggttgatg tgcatcgcgt cgatgccgag gtcgccgggc cgcacccggc cgacgatggc
     2581 gttgaaattg gccccgtcgc aatagacgag cccgcccgcc tcgtggacgg cgcggctgat
     2641 ctcggtcatc tccggctcga acaggccgca ggtattgggg ttggtgatca tcaccgcggc
     2701 gacgtcgggc ccgagccggg cctgcagggc ctcgaagtcg acccggccgc ggtccgtggc
     2761 ggggatgctc tcgacccggt agccggcgaa ggccgcggtc gccggattgg tcccgtgagc
     2821 gctttccggc accaggatca cctcgcgcgc gtcgccccgg gcctccagcg ccgagcggat
     2881 ggcgaggagg ccgcacagct cgccgtgcgc gcccgccttc gggctcatcg cgacgctgtg
     2941 catcccggtc agcctcacca gccactcgcc gagccggtgg atgacctcga acgccccctg
     3001 caccgtgtcc accggctgga gcgggtggat gtcggcgaag ccgggcatcc gggccacctt
     3061 ctcgcacagc cgcggattgt gcttcatcgt gcacgagccg agcggaaaca ggccgaggtc
     3121 gatcgcgtag ttctgccggc tgagccgggt gtaatggcgg acggcctccg gctcggtgag
     3181 gccggcgagg ccgatcggcc gggtccgctc gaggccgccg agcctgctcc ccggcgaagg
     3241 ccggggccga gtttctgcgc cggatcgggc ctcggccttc gccggggaac attggtcgaa
     3301 atccacgccc gtcgtgtccg aatcgccgat ctcgaacagc aggggctcct cgagcatcag
     3361 cgcccggttg ccggtatggg tgggcggcgc gccttcggcc gactccggcc gggtcggccg
     3421 tccttcgcgg ttcatgctca tgccagcgcc tccccgagcg cctcgaccag cgcctcgatg
     3481 tcctgacgat cggcggtctc ggtgaccgca acgaccagac cgttctccag cgcgtccgcg
     3541 ccgggataga gccgcccgag cgacactccg ccgagcaccc cttcgtccgc catcgccctc
     3601 accgccgggc gtgcctcctt cggcaggcgc agggtgaatt cgttgaagaa gctctcgttg
     3661 accagctcga ccccgggcag ggcgacgagc cgcccggcgg cctcgagcgc cctgcgatgg
     3721 ttcgcctcgg caagctggcg gagccccttc tcgccgagca gggtgaggtg gatggaaaag
     3781 gcgagcgcgc acagcaccga gctggtgcag atgttcgagg tcgccttctc gcgcctgatg
     3841 tgctgctcgc gggtcgacaa ggtgaggacg aagccgcgct tgccctcggc gtccaccgtc
     3901 tccccggcca ggcgccccgg catctgccgg acaaatttct cgcgggtcgc gaacagaccg
     3961 acatagggtc cgccgaaatt gagcccgacg ccgatcgact ggccttcgcc gacgacgatg
     4021 tcggcgccca tctcgcccgg agagcggacc aggccgagcg ccaccggctc ggtcaccacc
     4081 gcgatcagca gcgccccctt ctcatgcgcc cgctccgcca gctcggagag atcgccgatc
     4141 cggccgagga tgtcgggata ctggacgacg acgcagctcg tctcgccgtc gatcgcgtcg
     4201 agcagccggg cggtgtcggg ctccccggtc agctccggga tgcgcgtctc cagaaggtcg
     4261 ccggtgaacc tcgccatcgt ccgggcgacc gagacgtaat ggggatggag gccggaggag
     4321 aggatggcct tgccgcggcg ggtgatccgc cgggccatgc cgatcgcttc ccagcaggcg
     4381 gtcgagccgt catacatcga ggcgttggcg acgtcgcagc cgtagaggcg cgcgacctga
     4441 gtctggaatt cgaacaaagc ctgcagcgtg ccctgtgcga tctccggctg atagggcgtg
     4501 tagctggtca ggaactcgcc gcgctggatg aggtgatcga ccgaggccgg gatatggtgg
     4561 cgataggcgc cgcagcccag gaagaagggc gcatcgccgg cgacgaggtt cctgcgcgca
     4621 agggcggaga gccggcgctc gaccgccatc tcgctgaggt ggtcgggcag gcccgggatc
     4681 ttagcggcaa gacgcgccgc ctcggggacg tcgacgaaca gatcgtcgat cgaggacgcg
     4741 ccgacgacgc gaagcatgtc ctgccggtcc gtgtcggcga gggggaggta acgcattctt
     4801 ctactccgtt actcggccgg cttccgcatg gcgaagcgca gccgcacata gtcggcgacc
     4861 cagctgccgt cgggctggcg gagctgcggc tgcagccggg cctcgaccgc cgcggcgacc
     4921 tcgtcgcgat gcgcctccgg aacgccggcg acgtcgagcc agccggcgcg gaaggtcttc
     4981 acccacgcgg cgacgcccgc gggcaagagc gtcggccgcg ggatcagctg ggcctggatc
     5041 tcggtgaagc cggcgcaggc gtagagccgc acgaactcct cgcagccggg ataccattgc
     5101 ggatcctcgg ccggcaccga atagccgcgc tcgctcagct cggcgcggat gccggcgcgc
     5161 agcgccgcga tattgccctg gccgcccatc tcgccgacga agcggccgcc cggacggatc
     5221 atcgcgaaca cccccgaagc gacggcgtcg ggatcgagca
//
LOCUS       DASVQZ010000178         6624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_37439, whole genome shotgun sequence.
ACCESSION   DASVQZ010000178 DASVQZ010000000
VERSION     DASVQZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37439"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VF547_04925"
     CDS             <1..334
                     /locus_tag="VF547_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262197.1"
                     /translation="AAAAPLTAEQAVEAQQDQVRQAVGTAPCARDSGAGDIVVCGRRG
                     PDPNRLPLPVEPAPGERVRMLPGELPRAQTAYDTCLFGCPRPVTITDEDVRKYGGRIL
                     RHILGKDD"
     gene            414..1499
                     /locus_tag="VF547_04930"
     CDS             414..1499
                     /locus_tag="VF547_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262198.1"
                     /translation="MSVEAAPPSRLNGARAWADWAARALVAVLVTYAFIVVLLVATAQ
                     QKVDDALTKEAVGYDYSVAVRYYFGKDSLKNVVGENSQQIKEATARLRAANDRFQSAN
                     RALTAEAADLKEDLDRLTAAGCPAPPAPETPPTPAELVGMAVATQHCAAERGEAVPAI
                     PPIAREVLAGQRAVQKSLDDLAGLKRDSDDLQDRLDLLQAERNQIDKQLEAAARSGDI
                     IAVLKVFEDSRWPLAKQLVYVPPALTGIILASVSGLFGALLITLVLFVYPDNRYRFTR
                     TKSYYGRILLGGLIALGVFVLMFSGVAVLAGPNASGSAQNLIAYGGIGILSGMFSDQA
                     AGWLSDRSVFKPENASEAAELAEEAAA"
     gene            1602..2225
                     /locus_tag="VF547_04935"
     CDS             1602..2225
                     /locus_tag="VF547_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262199.1"
                     /translation="MAKLYLAALLAVAAAAAHAQSPKVTLEPSPDSAEQRKASQKLTA
                     CLAEARPRWARNTLSRPYLSDAQAFDASAAVRGRDTCLTSRDGVEVTFRTSLMVGSLA
                     EHFLKDEIRRADARRLAEKLSTLPPRNVTEDFALCVTARNPAAASDLALSEPGSAAEE
                     DAARRVFSYVEPCRQENEELTVDLQALRALVAAALYRGVNEVLTAGS"
     gene            complement(2277..2558)
                     /locus_tag="VF547_04940"
     CDS             complement(2277..2558)
                     /locus_tag="VF547_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262200.1"
                     /translation="MRILTVAALAIGLGALGSGPALAADDGASKPKKAKDDTSRRVCR
                     SVVPTGSRMSGRICRTQAEWDAAMDKSQDNILRHQTTKQTLLQQAQGPM"
     gene            complement(2652..3830)
                     /locus_tag="VF547_04945"
     CDS             complement(2652..3830)
                     /locus_tag="VF547_04945"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX8262201.1"
                     /translation="MSHDFYRIRRLPPYVFGEVNAMKAAARARGEDIVDLGMGNPDGA
                     PPAHVIDKLAEVARNPRAHRYSASRGIAGLRKAKAGYYARRIGVELDPEREVIVTLGS
                     KEGLANLAQAITAPGDVVLAPNPSYPIHQFGFIIAGAAIRSIPAAPGPGFFDMLERAV
                     RYTVPKPKVLVIGYPSNPTAYTADLPFYERVVGFAREHDLWVISDLAYAEIYFGDSPT
                     PSILQVPGAREVAIEFTSMSKTYSMAGWRIGFAVGNAELVAALGRVKSYLDYGAFTPI
                     QAAAVAALNGPQDIVEANRRLYRSRRDCLVECFGRAGWEIPPPEASMFAWAPIPEPFR
                     ALGSMEFAKRLLAEAHVAVSPGVGFGEEGEGFVRIALVENEQRIRQAARGVKRYLGAG
                     "
     gene            complement(4052..4345)
                     /locus_tag="VF547_04950"
     CDS             complement(4052..4345)
                     /locus_tag="VF547_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262202.1"
                     /translation="MDLEQDKVRNTLEGQIDDLVKARRASSDPKEKEALSSAISRLER
                     EVDLLNLTAADALPAKVDAIVKSLQGVLQANSLDAVSALGRSIEKIEALTKQG"
     gene            complement(4349..5206)
                     /locus_tag="VF547_04955"
     CDS             complement(4349..5206)
                     /locus_tag="VF547_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262203.1"
                     /translation="MFRTVLALAMLTLGSACAQVPKPELQAYSAAFEATRSAAEPMLA
                     DYAVAERGVRLDVMETDPDRHFDGFFPTFDPGDAAALTTVSLPPGAAAIDRSFNAIKA
                     YNDTLVALAENRNIDEARGQVGVIIDNLGGIAPPLAGAAPAVKPVADLLITALSPAIQ
                     ADNRAQFKRIVLDGEPHVQALIDTLRDYTPTQYSITTTALRKKARLDPPPPDRDATVA
                     RINAWQKAFADYVALLDAMKQRLSDLREAVENPRSEPLLQRAAAGSAELRSFADALRL
                     SLAQVRAQP"
     gene            5452..6492
                     /locus_tag="VF547_04960"
     CDS             5452..6492
                     /locus_tag="VF547_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262204.1"
                     /translation="MESSESRLAPSPEPASDDSVSPRNLEPALDRLDFETVPLRRRVD
                     GLTPEKQRRYVEALADSGIAREAAARVGLSEQAINRVRRRADARSFDDACEAAQLFGA
                     RRLRSIAYERAIEGTLKGHYYRGERVGEERVYDNRLLIYLLGKTEHLLRQEERKSQAI
                     CEDWEPRMDALEQGLEPPGPAAPAPELSETGWGSPVWEDEETGILFTTFPPPEGFEGE
                     EQGVWDGIQWYCRTLTPDEQAVMDAGDAEEMAALRAEECARRDRFFGFAGDGISAPGE
                     AETNETSKALEEAEETSAEGALEFKSTVPPSPTAGWDRAPSPAGRPGEPKAMHSEVRG
                     PPASAGPGSNPE"
BASE COUNT         1046 a         2371 c         2206 g         1001 t
ORIGIN      
        1 cgccgcggcg gcgccgctca ccgcggagca ggccgtcgag gcccagcagg accaggtccg
       61 tcaggccgtc ggcaccgcgc cctgcgcccg cgattcgggc gcaggcgaca tcgtcgtctg
      121 cggacgccgc ggtcccgatc cgaaccgcct cccgcttccc gtcgagccgg cgcccggcga
      181 gcgcgtgcgc atgctccccg gcgagctgcc gcgcgcccag accgcctacg acacctgcct
      241 cttcggttgc ccgcgcccgg tcaccatcac cgacgaggac gtccgcaaat atggcggccg
      301 gatcctccgc cacatcctcg gcaaggacga ttagtgacag tcactaaatg cggagagaca
      361 atctccctca accgatttat ggatatggcc gacgctccgg ggggagagaa gccatgtcgg
      421 tcgaagccgc gccaccgagc cgcctcaacg gcgcccgcgc ctgggccgac tgggccgccc
      481 gagccctggt ggcggtcctc gtcacctatg ccttcatcgt cgtcctcctc gtcgccaccg
      541 cccagcagaa ggtcgacgac gccctcacca aggaggcggt cggctacgat tacagcgtcg
      601 ccgtgcgcta ttatttcggc aaggacagcc tgaagaacgt cgtcggcgag aacagccagc
      661 agatcaagga ggcgaccgcc cggctgcgcg ccgccaacga ccggttccag tcggccaacc
      721 gggcgctgac cgccgaagcg gccgacctga aggaggatct cgaccggctc accgcggccg
      781 gctgcccggc cccgcccgct cccgagacgc cgccgacccc ggccgagctg gtcggcatgg
      841 cggtcgccac ccagcattgc gccgccgagc ggggcgaggc cgttccggcc atcccgccga
      901 tcgccaggga ggtgctcgcc ggccagcgcg ccgttcagaa gtcgctcgac gaccttgccg
      961 gcctgaagcg cgactccgac gacctccagg accggctcga tctgctccag gccgagcgca
     1021 accagatcga caagcagctc gaagcggccg cccgctccgg cgacatcatc gccgtcttga
     1081 aggttttcga ggacagccgg tggcccctcg ccaagcagct cgtctacgtc ccgccggcgc
     1141 tgaccgggat catcctcgcc tcggtctccg gcctgttcgg ggcgcttctc atcaccctcg
     1201 tcctgttcgt ctatccggac aatcgctaca ggttcacccg gaccaaatcc tattacggcc
     1261 ggatcctgct gggagggctg atcgcgctcg gagtgttcgt gctgatgttc agcggagtcg
     1321 ccgtcctcgc cggccccaat gcgagcggca gcgcccagaa cctcatcgcc tatggcggca
     1381 tcggcatcct atcgggaatg ttctcggacc aggccgccgg ctggctctcc gaccgctcgg
     1441 tgttcaagcc ggaaaatgca tcggaggcgg ccgagctggc cgaggaggcc gcggcctgag
     1501 cggctctcgc cgtccaaaag cgggaggccg tcacggcggc tcttccgttc cgcgatcgaa
     1561 acgcgcatca tgactccgct catccagggg agactcgact catggccaag ctttatctcg
     1621 cagctctgct ggcggtcgcc gccgccgccg cccacgccca gtcgcccaag gtgacgctcg
     1681 agcccagccc cgattccgcg gagcagagga aggcgtcgca gaagctcacc gcttgcctcg
     1741 ccgaggctcg gccgcgctgg gcgcgcaaca cgctctcccg tccctatctg agcgacgccc
     1801 aggccttcga cgcctcggcc gccgtgcggg gaagggacac ctgcctcacc agccgggacg
     1861 gcgtcgaggt gaccttcagg acctcgctca tggtcggcag cctcgccgag catttcctga
     1921 aggacgagat caggcgcgcc gatgcccggc ggctggcgga gaagctgtcg accctgccgc
     1981 cgcgcaacgt caccgaggat ttcgctttgt gcgtgacggc gcgcaatccc gccgcggcca
     2041 gcgacctcgc gctcagcgag ccgggcagcg ccgccgagga ggacgccgcc cgcagggtct
     2101 tctcctatgt cgagccctgc cggcaggaaa acgaggagct gaccgtcgac ctgcaggcat
     2161 tgcgcgcgct tgtcgccgcc gccctctatc ggggggtcaa cgaggtgctg acggccggga
     2221 gctgagcttc ggccggaccg cggccggtcg tccggccgcg ggcccgaccg gcgggctcac
     2281 atggggccct gggcctgctg gagcaaagtc tgcttggtgg tctggtgacg caggatgtta
     2341 tcctgcgact tgtccatggc ggcgtcccac tccgcctggg tgcggcagat gcgcccggac
     2401 atccgggagc cggtcggaac cacgctccgg cacacccggc ggctggtatc gtccttcgcc
     2461 ttcttgggct tcgacgcgcc gtcgtcggcc gctagggcgg gaccggagcc cagggcaccc
     2521 agcccgatcg ccagcgctgc gactgtcaag atccgcatgc cacccatatc aactctccct
     2581 ttccgctgtc cgctcggcag cgccgaatcg gaagcgtaca agatattggt cgcaaaaggg
     2641 agcctgagct ttcaaccggc gccgagatac ctcttcaccc cccgcgccgc ctgccggatg
     2701 cgctgctcgt tctccacgag cgcgatccgc acgaacccct cgccctcctc gccgaacccc
     2761 acgcccgggc tgaccgcgac atgagcttcg gcgagcagcc tcttggcgaa ctccatcgag
     2821 ccgagcgccc ggaagggctc cgggatcggc gcccaggcga acatcgaggc ctcgggcgga
     2881 gggatctccc agccggcgcg gccgaagcat tcgaccaggc agtcgcgtcg cgaccgatag
     2941 agccggcggt tggcctcgac gatatcctgc ggcccgttca aggcagcgac cgccgctgcc
     3001 tggatcggcg tgaacgcgcc ataatccaga taggatttga cccgcccgag cgccgctacc
     3061 agttcggcat tgccgaccgc gaagccgatc ctccaccccg ccatcgaata ggtcttcgac
     3121 atcgaggtga actcgatcgc gacctcgcgc gctccgggca cctgcaggat ggacggggtc
     3181 ggcgagtcgc cgaaatagat ctccgcatag gcgaggtcgc tgatcaccca cagatcgtgc
     3241 tcgcgggcga agccgaccac gcgctcgtag aagggcaggt cggcggtata ggcggtgggg
     3301 ttcgagggat agccgatcac cagcaccttg ggcttgggca ccgtgtagcg caccgcgcgc
     3361 tcgagcatgt cgaagaatcc cggccccggc gccgccggga tcgagcggat cgccgccccg
     3421 gcgatgatga agccgaactg gtggatcgga taggagggat tgggcgcgag cacgacgtcg
     3481 cccggcgcgg tgatcgcctg ggccaggttc gccagcccct ccttcgagcc cagcgtgacg
     3541 atcacctccc gctccgggtc gagctcgacc ccgatgcgcc gagcataata gcccgccttc
     3601 gccttcctca gcccggcgat cccgcgcgaa gccgaatagc ggtgcgcccg cgggtttcgc
     3661 gccacctcgg cgagcttgtc gatcacatgg gcaggcggcg ctccgtcggg attgcccatg
     3721 ccgaggtcga cgatgtcctc gccccgggcc cgagccgccg ccttcatcgc gttcacctcg
     3781 ccgaagacgt agggcggcag ccggcggatc cggtagaaat cgtggctcat gccccgccct
     3841 taggagcgcc ccccgaggcc gttcaagcgc ccttctgaag ttcctcccct gcatttgcag
     3901 gggaggggga ccaggcgaag cctggtggag gggccttgcc gaatctccgg caaggaaccc
     3961 caccgccgcg ctgcgccgac ggcgcagttt atcctgggcg gcgtcgaagg ggggcggtcc
     4021 ccctccccgc aaatcgcggg gagaatgaag ctcagccctg cttggtcagc gcctcgatct
     4081 tctcgatcga gcgcccgagc gccgagacgg cgtcgaggct attggcctgg aggaccccct
     4141 ggagcgactt cacgatcgcg tcgaccttgg ccggcagggc gtcggcggcg gtgaggttga
     4201 gcaggtcgac ctcgcgttcc agcctcgaga tcgcgctcga cagcgcctct ttctccttcg
     4261 ggtcgctgct ggcgcgccgg gccttgacga gatcgtcgat ctgcccctcg agcgtgttgc
     4321 gaaccttgtc ctgctccaaa tccacctcct acggctgggc gcgcacctgc gcgagggaaa
     4381 ggcgcagcgc gtcggcgaag gagcgcagtt ccgccgagcc ggcggccgcc cgctgcagaa
     4441 ggggctcgct gcgcggattc tcgaccgcct cgcgaaggtc cgagagcctc tgcttcatcg
     4501 cgtcgagcag ggcgacgtaa tcggcgaagg ccttctgcca ggcgttgatc ctcgccaccg
     4561 tcgcgtcgcg atccggcggc ggcgggtcca gccgggcctt cttgcgaagc gcggtcgtgg
     4621 tgatgctgta ttgcgtaggc gtatagtcgc gcagggtatc gatcagcgcc tggacgtggg
     4681 gctccccgtc gaggacgatt cgcttgaact gcgcccggtt gtcggcctgg atcgccgggc
     4741 tgagggcggt gatcagcagg tcggcgaccg gcttcaccgc gggcgcggcg ccggccagcg
     4801 gcggcgcgat gccgccgaga ttgtcgatga tcacgcccac ctggccgcgg gcttcgtcga
     4861 tgttgcggtt ctcggcgagc gcgacgagcg tgtcgttata ggccttgatc gcgttgaagc
     4921 tccggtcgat cgccgccgcc ccggggggca ggctgacggt ggtcagggcc gcggcgtcgc
     4981 ccggatcgaa ggtcgggaag aagccgtcga aatgacggtc ggggtccgtc tccatcacgt
     5041 cgaggcggac tccgcgttcg gcgacggcat aatcggccag catcggctcg gccgccgagc
     5101 gggtcgcttc gaaggcggcc gaataggcct gcagctccgg cttcggcacc tgcgcgcagg
     5161 cgctgccgag cgtcagcatc gccaaagcca agacggtcct gaacatccgc ttccccccgc
     5221 gagaaagacc cgaggctgac cttcgcccgg ccgggagtca aggcgccacg cccgccggcc
     5281 tcgagcctcg ccccaattcg ctccgcaacg gcccgattcc ggccacagga aacttccttc
     5341 ggtgccaggc accgcttcct tcggtgccag gcaccgatcc tcaaggcttt gaaacgaagg
     5401 gcgcgggaaa taggatttgt ccttttgttc ttcttatgtt ctaatgctcg gatggaaagc
     5461 tccgagtcgc gccttgcgcc atcgcccgag ccggcgtccg atgattccgt ctcgccccgc
     5521 aacctcgagc cggccctcga ccgcctcgac ttcgagaccg tgcccctccg ccgccgcgtc
     5581 gacggactca cccccgaaaa gcagcgccgc tatgtcgagg cgctcgccga cagtgggatc
     5641 gcccgcgagg ccgcggcccg ggtcgggctc agcgagcagg cgatcaaccg ggtccgtcgc
     5701 cgtgccgacg cccgctcgtt cgacgacgcc tgcgaggccg cacagctgtt cggagcgcgc
     5761 cggcttcgct cgatcgctta cgagcgcgcc atcgagggga ctctcaaggg ccattattat
     5821 cgtggcgaac gggtcggcga ggagcgggtc tacgacaacc gcctgctcat ctacctgctc
     5881 ggcaagaccg agcatctcct ccggcaggag gagaggaaga gccaagcgat ttgcgaggat
     5941 tgggagcccc gtatggacgc tctcgaacag ggtctcgagc cgccggggcc ggccgcaccc
     6001 gcgccggagc tcagcgagac aggctggggc tcgcccgtct gggaggacga ggagaccggc
     6061 atcctgttca cgacctttcc gccgcccgag ggattcgagg gcgaggagca aggcgtctgg
     6121 gacgggatcc aatggtattg ccgcaccctc acgcccgacg agcaggcggt gatggacgct
     6181 ggcgacgccg aggagatggc ggccctgcgg gctgaggaat gcgcccgccg cgaccgtttc
     6241 ttcggcttcg ccggggacgg catctccgcc cccggggaag ccgaaactaa tgaaacttcc
     6301 aaagccttgg aggaagcgga ggaaacatcc gcggaaggcg cgctcgaatt taaatcgacg
     6361 gtgcccccga gcccgaccgc gggttgggac cgcgcgccat ccccggccgg tcgtccaggc
     6421 gagccgaaag ccatgcactc cgaggttcga ggaccgccgg cgtcagcggg cccggggtca
     6481 aacccggaat gacggtcggg gaaccggcca tacgcagccg gcggcggcgc gccccgccga
     6541 acctttcccc cgccccgcgc atttcctaga tagcgcgcga caggagtgac caatggccga
     6601 ccaggacacc aaggcccccg aggc
//
LOCUS       DASVQZ010000179         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_518603, whole genome shotgun sequence.
ACCESSION   DASVQZ010000179 DASVQZ010000000
VERSION     DASVQZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518603"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3506
                     /locus_tag="VF547_04965"
     CDS             <1..>3506
                     /locus_tag="VF547_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262205.1"
                     /translation="TSFLDDVLGRTSIAGVGLRSISTRAGDQQIGVAYDFDNNRLVLA
                     DVDGAGGGSIRLTGKIYSTTTYGALQVNSGYGDIQIRNESTAALRIGDIYTGTNSLGT
                     IQITDLNKTGHQGSPLTTWFVYETAGNRLTTYDNAATGSLNYTTLAPRSVIDGRTTSY
                     APEAGYRYNWQMSRSASRFVLPNSVPDEFAVYRNSTSDWQWTSSDWQIGGQSFDKAPR
                     TGMPFFEQTVSGKITDRNRTRDGYGNFAFSTTDGAFVWGNVQHTKYVEAVTGIEITNK
                     MSVKADNPIAIGFTGNANARVDIQSKGDIAVAGRIGNSTGTASIVGTGAATGIVDAQG
                     MIVTRDLTLGASGSGYVGTADKPLHVSASGTIAANAPGGVALAVDGTATLGQVSATGD
                     VQIAATGAILGKDGTASVSGRNISLAAAGGAIEGAGGAPLTIQTQTTQLQDRILGGEL
                     TASARDSINLRQPTGDLRLRHVTSQTGDVTLEASTGSIRNAVTQFTVDEDNLKRLADV
                     WSELGLTDPQSVLKAVTAFERFVATQQAERARILAQVEMVDGKVVGLGKPGASADEIA
                     LVTAIYGGQLKARDKLAADPTADQIKQYIQQRFDAIEAYARETFGADRPAGFDSADPT
                     QAFAYLLDPASPRYRELTRGVWTETQLTQSLSEAALTPVSNTQILLQEPNVKGRSVTL
                     VAAQGSIGQNLEQLVIDAQVGRLLTPAERAALAAAGPGDVRVEASAGGGHQLTVSQQR
                     LVNVAATGDVTARAATDLYLGSNGNLRAAAIETAGTARIAISGSISDVAGAQRAAVTA
                     GDLLIEAGNGSIGTSAAPLRVQLSGRLLSARAGIDVHIKQLQGDLRTGVAFAGGLLSL
                     EAGSGSILSAFANNEAVRLQGDSIALAATGDLGTAANRLQVEVASTGTLSGSGRAAVI
                     HSPEGLLRVGALSLSGAATISGADLAFVGAFDGASLVARAPNGDLDVTGSVATSGNAA
                     LIAGNVRLGDGATVTSGGNLSVEAGDARFGELASATVGGTSRIAARSGLEFAAGARLS
                     STGAADLTAGTAIQLGDSAAIASSGDALGLGAASLAAGDGVSLSAATVLQVQTGTGAL
                     VFGKESRLSGGSVALSSAGALALGDGSRANGAAGVALLAGNGAALGDRVALTGSAGDV
                     TVTTTAGDLVIGEDAALNGSRV"
BASE COUNT          579 a         1299 c         1151 g          477 t
ORIGIN      
        1 cacgtccttc ctcgacgatg tcctcggccg caccagcata gctggcgtcg gcttacgctc
       61 catctcgact cgggcaggcg accagcagat cggagtcgcc tacgatttcg acaacaaccg
      121 cctcgtcctg gccgatgtcg acggcgccgg cggcggctcc atccggctca ccggcaagat
      181 atactccacc accacctacg gcgcgcttca ggtgaacagc ggctacggcg acatccaaat
      241 ccgcaacgag tcgacggcgg cgctgaggat cggcgatatc tacaccggaa cgaactcgct
      301 cggcacgatc cagatcaccg acctcaacaa aaccgggcat caggggagcc cgctgacgac
      361 ctggttcgtg tacgaaaccg cgggaaacag gctcaccacc tacgacaacg ctgccaccgg
      421 cagccttaac tatacgaccc ttgcccctcg aagcgtcata gacgggcgga cgacctcata
      481 cgcgccggag gccggctatc gctacaattg gcagatgagc cggagcgcgt cccgtttcgt
      541 cctccccaac agcgttccgg acgaattcgc cgtctatcga aactcaacct ccgactggca
      601 atggacctcg agcgactggc agatcggcgg ccagagcttt gacaaggcgc cccgcacggg
      661 catgcccttc ttcgagcaga cggtgagcgg taagattaca gatcggaacc ggacgcgtga
      721 cggctacggc aatttcgcat tcagtacgac cgacggcgcc ttcgtctggg gcaacgttca
      781 gcataccaaa tatgtcgagg cggtcacggg catcgagatc accaacaaga tgagcgtcaa
      841 ggccgacaat ccgatcgcca tcgggttcac cggcaatgcc aacgcccggg tcgacatcca
      901 gtccaagggc gatatcgccg tcgccggccg gatcgggaat tcgaccggga ccgcttcgat
      961 cgtcggcacc ggcgccgcga ccggaatcgt cgatgcgcag ggaatgatcg tcacccgcga
     1021 cctgacgctc ggcgcgagcg gaagcggcta tgtcgggacg gcggacaagc cgctgcacgt
     1081 ctccgcgagc ggcacgatcg cggccaacgc ccccggcgga gtcgccctcg ccgtcgacgg
     1141 cacggcgacg ctcggccagg tcagcgcgac gggcgacgtc cagatcgcgg cgaccggcgc
     1201 aatccttggc aaggacggga ccgcttccgt ctccggccgg aacatcagcc ttgccgccgc
     1261 cggcggagcg atcgagggcg ccggcggggc gccgctcacc atccagaccc agaccaccca
     1321 gctccaggac cggatcctgg gcggcgagct caccgcctcg gcccgcgaca gcatcaacct
     1381 tcgccagccg accggcgatc tccgcctccg gcatgtgacg tcgcagaccg gcgacgtgac
     1441 gctcgaggcg agcacgggca gcatccgtaa cgccgtcacc cagttcaccg tcgacgaaga
     1501 caatctgaag cggctcgcgg acgtctggag cgagctcggc ctcaccgatc cccaatcggt
     1561 cctgaaggcg gtgacggcct tcgagcgctt cgtagccacc cagcaggcgg agcgggcccg
     1621 gatcctggcc caggtcgaga tggtggacgg caaggtcgtc ggcctcggca agcccggagc
     1681 gagcgcggac gagatcgccc tggtcaccgc catctacggc ggccagctca aggcgcgcga
     1741 caagctcgcc gccgatccca ccgccgacca gatcaagcag tacatccagc agcgcttcga
     1801 tgcgatcgag gcctatgccc gcgagacttt cggggcggac cgcccggccg gcttcgattc
     1861 ggccgatccc acgcaggcct tcgcctacct gctcgatccc gcctcgccgc gctatcggga
     1921 gctcacccgg ggtgtctgga ccgagaccca gctcacccag tcgctcagcg aagcggccct
     1981 gaccccggtc agcaacaccc agatcctcct tcaggagccc aacgtcaaag gccgctcggt
     2041 gactctcgtc gccgcgcagg gatcgatcgg ccagaatctc gagcagctcg tcatcgatgc
     2101 gcaggtggga cggctcctca cccccgccga acgcgccgcc ctcgccgcag ccgggcccgg
     2161 cgacgtccgg gtcgaggcca gtgccggcgg cgggcaccaa ctgacggtca gccagcagcg
     2221 gctggtgaac gtcgccgcca ccggcgatgt caccgccagg gcggccaccg acctctatct
     2281 cggctccaac ggcaatctgc gcgcggccgc gatcgagacc gccggcaccg cccggatcgc
     2341 catctccggg tcgatcagcg acgtggcggg cgcccagcgg gcggccgtca ccgccgggga
     2401 ccttctgatc gaggccggca acggctcgat cggcacctcg gccgcgccgc tgcgcgttca
     2461 gctttcgggc cggctgcttt cggcccgggc cggaatcgac gtccacatca agcaattgca
     2521 gggcgacctg cggacaggcg tcgccttcgc gggcggcctg ctcagcctcg aggcgggcag
     2581 cggctcgatc ctttccgcct tcgccaataa cgaggcggtg cggctgcagg gcgattcgat
     2641 cgccctcgcc gcgaccggcg atctcggcac cgccgccaac cgtctccagg tcgaggtcgc
     2701 cagcaccggg accctctccg gctcgggccg cgcggcggtg atccacagcc ccgaagggct
     2761 cctgcgcgtc ggcgccctca gcctgtcggg cgcggcgacg atcagcggcg ccgaccttgc
     2821 cttcgtcgga gcattcgacg gcgcctcgct ggtcgcgcgc gcgccgaacg gcgatctcga
     2881 cgtgaccgga agcgtcgcca cgagcggcaa tgccgctctg atcgcgggca atgtgcggct
     2941 cggtgacggt gcgacggtca ccagcggcgg caatctctcg gtcgaggcgg gcgatgcccg
     3001 cttcggcgag ttggcgtctg cgacggtcgg cggcaccagc cggatcgcgg cccgcagcgg
     3061 cctcgagttc gccgccggcg ccaggctctc ctcgaccggt gcggccgacc tcaccgccgg
     3121 cacggcgatc cagctcggcg actccgccgc gattgcgagc agcggcgacg ccctcggcct
     3181 cggcgcggcg tcgctcgccg ccggcgacgg tgtcagcctg tccgccgcga ccgtcctgca
     3241 ggtgcagacc gggaccggcg ccctcgtctt cggcaaggag agccggctga gcggcggcag
     3301 cgtcgcgctg tcgtccgccg gcgccctcgc cctcggcgac gggtctcggg cgaacggcgc
     3361 ggccggcgtc gcgcttctcg ccggcaacgg agcggcgctc ggcgaccgcg tcgcactcac
     3421 cggcagtgcc ggcgacgtga cggtgacgac gaccgccggc gacttggtca tcggcgagga
     3481 cgccgctctg aacggcagcc gggtcg
//
LOCUS       DASVQZ010000180         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_327102, whole genome shotgun sequence.
ACCESSION   DASVQZ010000180 DASVQZ010000000
VERSION     DASVQZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327102"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1548
                     /locus_tag="VF547_04970"
     CDS             256..1548
                     /locus_tag="VF547_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8262206.1"
                     /translation="MRILLIGASGLIGSAVAARLKRDGHEIVAVGRGGGPAARRVPAD
                     RRVTLDLREATAPEAWAPLFDGVDAVVNCAGVLQDSPRDSPARLHRDMPIALWRACEA
                     AGVRRVIHFSALGVDRGDVTPFSRTKREGDEALAASGLDWVILRPSVVVGRPAYGGSA
                     LFRGLASLPVFPRPAETGPVDVVQLDDVAETVARLVDPGAAARVTLELAGPDRLAFPE
                     IVAAYRRWLGWRPARSFTAPGWLMGLAYRAGDGLARLGWRPPIRSTAGREIRRGATGD
                     ADPWRRATGIEPQPLAAMLEANPASVQERWFARLYLLKPLALGTFTLFWLLTGLVSLG
                     PGYRRATDYMLAGGGGALSEPSVIAGGLADIAVALGIAWRPTAKPALWAALGLSLFYL
                     VAGTILLPGLWADPLGPMMKIWPILALNLVCLAILDDR"
     gene            1545..2021
                     /locus_tag="VF547_04975"
     CDS             1545..2021
                     /locus_tag="VF547_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEX8262207.1"
                     /translation="MTAYYLLKWLHVIGASVLLGTGAGIAFFMLMAHRGGEPRVIAAV
                     ARIVVLADYLFTATAVVAQPATGLLLARTVGYSLAEGWILASIALYLFTGAFWLPVVW
                     MQSRMRDLARTAAETGTALPDEYHRLFRRWFAFGFPAFTAVLAIFWLMIARPELGI"
     gene            2018..>2364
                     /locus_tag="VF547_04980"
     CDS             2018..>2364
                     /locus_tag="VF547_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262208.1"
                     /translation="MKDDRLKLTSRLLIGGIAGFVGTMAMTAAMRRLHRRLPDEERYP
                     LTPREIVDSGSRQLGVPVAGEVAKDLTTAAHFAYGAACGAVIAAMNPDPKKRTGAAAG
                     AAVWLASYMGWIPA"
BASE COUNT          269 a          884 c          827 g          384 t
ORIGIN      
        1 tgggtgatgc gcgactggct gaacggcttt gacgcccgga tcgacctcgg gccgactccg
       61 ttcctcgtcg cggggatgct cgctctgggc atcgccgtcg ccaccatcgc cggccacgcc
      121 ttcaaggtcg cccgctccaa cccgatccac gcgcttcggt acgaatagcc tgcgtcattg
      181 cgagcggagc gttgtcgctt cgctcctcgc aatgcgggag aggaacgcct cagcgcgctc
      241 cccgttacgg gagcgatgcg catcctcctc atcggcgcct ccgggctgat cggctcggcc
      301 gtggccgccc ggctgaagcg cgacgggcac gagatcgtcg ccgtcggccg cggcggcggg
      361 ccggcggcgc ggcgggtgcc ggccgatcgc cgggtgacgc tcgatctgcg cgaggcgacg
      421 gcgccggagg catgggcccc gctcttcgac ggcgtcgacg cggtggtgaa ttgcgccggc
      481 gtcctccagg acagcccgcg cgactcgccg gcccgtctgc accgcgacat gccgatcgcc
      541 ttgtggcgcg cctgcgaggc cgccggagtg cggcgggtga tccatttctc ggcgctcggg
      601 gtcgaccgcg gcgacgtcac ccccttctcg cggaccaagc gcgaaggcga cgaggccctg
      661 gcggcgagcg ggctcgactg ggtcatcctg cgtccctcgg tcgtggtcgg ccggcccgcc
      721 tatggcggca gcgcattgtt ccgcggcctc gcctcgctgc cggtcttccc gcgcccggcc
      781 gagacggggc cggtcgacgt cgtccagctc gacgacgtcg ccgagacggt ggcccggctg
      841 gtcgatccgg gcgccgccgc ccgggtcacg ctcgagctgg cgggaccgga ccggctggcc
      901 tttcccgaaa tcgtcgccgc ctaccgccgc tggctcggat ggaggcccgc gcgcagcttc
      961 accgcgcccg gctggctgat gggcctggcc tatcgcgccg gcgacggcct cgcccggctc
     1021 ggctggcggc cgccgatccg gagcaccgcc ggccgcgaga tccgccgggg cgcgacgggc
     1081 gacgctgacc cgtggcggcg agcgaccggg atcgagccgc agccgctcgc cgcgatgctc
     1141 gaggcgaacc cggcgtcggt gcaggagcgc tggttcgccc ggctctacct gctcaagccg
     1201 ctggcgctcg gcaccttcac cttgttctgg ctgctgaccg ggctggtctc gctcggcccc
     1261 ggctaccggc gcgccaccga ctatatgctc gccggcggcg gcggcgccct cagcgagccg
     1321 agcgtgatcg ccggcgggct cgccgacatc gccgtcgccc tcggcatcgc ctggcggccg
     1381 acggcgaagc ccgctctgtg ggcggcgctc ggcctctcgc tcttctacct ggtcgccggg
     1441 acgatcctgc tccccgggct ctgggccgac ccgctgggcc cgatgatgaa gatctggccg
     1501 atcctggcgc tcaacctcgt ctgtctcgcc atattggatg atcgatgacg gcctactacc
     1561 tgctcaaatg gctgcacgtg atcggcgcct ccgtcctgct cggcaccggt gcgggcatcg
     1621 ccttcttcat gctgatggcc catcgcggcg gcgaaccccg ggtgatcgcc gccgtcgccc
     1681 ggatcgtcgt cctcgccgat tacctgttca ccgccacagc ggtcgtcgcc cagccggcca
     1741 ccggcctgct gctcgcccgg acggtcggct attcgctcgc cgagggctgg atcctcgcgt
     1801 cgatcgctct ctacctcttc accggcgcct tctggctgcc ggtggtatgg atgcagtcgc
     1861 ggatgcgcga cctggcgcgg acggcggccg agaccgggac ggcgcttccc gacgaatatc
     1921 accgcctgtt ccgacggtgg ttcgcattcg gcttcccggc ctttacagcg gtgctcgcca
     1981 tcttctggct gatgatcgct cgaccggagt tggggatttg aaggacgacc gcctcaagct
     2041 gaccagccgc ctcctgatcg gcggcatcgc cggtttcgtc gggacgatgg cgatgaccgc
     2101 ggcgatgcgg cgccttcacc gccgcctgcc cgacgaggag cgctatccgc tgactccgcg
     2161 cgagatcgtc gattcgggct cgaggcagct cggcgtgccg gtcgccggcg aggtcgccaa
     2221 ggacctcacc accgccgccc atttcgccta tggcgcggct tgcggggcgg tgatcgcggc
     2281 gatgaacccc gatcccaaga agcgcaccgg cgccgcggcc ggcgcggccg tatggctggc
     2341 cagctacatg ggctggatcc cggc
//
LOCUS       DASVQZ010000181         9446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_424194, whole genome shotgun sequence.
ACCESSION   DASVQZ010000181 DASVQZ010000000
VERSION     DASVQZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424194"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VF547_04985"
     CDS             complement(<1..540)
                     /locus_tag="VF547_04985"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8262209.1"
                     /translation="MALARANWNYPTTIRFGAGRAAELGDSCRELGIERPLLVTDPAL
                     AKLPMVQAALAATGAGLFCDIHPNPVGADVEAGLAVYRGGSHDGVVAFGGGSALDVGK
                     VVAFMSGQARPLWDFEDKEDWWSRADPAGIAPVIAVPTTAGTGSEVGRAGVILDEAES
                     VKKIIFHPRMMPAAVILDPE"
     gene            complement(540..1937)
                     /locus_tag="VF547_04990"
     CDS             complement(540..1937)
                     /locus_tag="VF547_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8262210.1"
                     /translation="MSGWRTNGHLNEIVCISPVNGREYARRPMATDAEVDKALDAARR
                     AQREWPRTPLDERCATMLRFLEAMEALNPAIVPELAWQMGRPVRYGGELRSLAERVRA
                     MVDLAPSALAPFVAGAGRSVVRVSAGLVLVIAPWNYPFLTAANTIVPALLAGNAVILK
                     HASQTPLAGERFAEALGRSGLPEGLFANLFLSHEQTARLLGAGAVDHVTFTGSVEAGK
                     AVERAAAGSFITLTLELGGKDPAYVREDAELAGTVDGLVDGSFFNSGQSCCGVERIYV
                     HEAVWEAFLDSFVAATSQYRLGNPLDRETSLGPMVSGGAADLVRAQVRQAIEAGATGH
                     LPPQLFPTDSGEGPYLMPQVLTDVDHGMAVMREETFGPVIGIMAVRNDEEAVRLMNDS
                     RYGLTASIWTRDLQAAERLAARVEAGTVFANRCDYLDPLLAWTGVKDSGRGASLGRHG
                     FESVTRPKSLHLRSS"
     gene            complement(2059..3411)
                     /locus_tag="VF547_04995"
     CDS             complement(2059..3411)
                     /locus_tag="VF547_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262211.1"
                     /translation="MAKGDLVKRIRERDPACAKVGVFDVDGIFRGKYMAPDKLASALA
                     GGFGFCDVVLGWDSSDQLYDNAAFTGWHTAYPDAEVRLLPDTERTMPFEGGMPLILGE
                     FAGAAEAICPRGVLRRVLDRAAGMGCRVKAAAEFEFFVFEETPQSVRDKGYRQLKPIT
                     PGHFGYSMLRSGVHADFYAELWSTCEAMRIELEGLHTETGPGVIEGAIRVDDALEAAD
                     KAALFKTIVKILCQRRGWMATFMAKWSNDWPGQSGHLHMSLVDAGSGRSRFHDEGAEL
                     GMSQEMRWFVGGQQALMPELLAMVAPTVNAYTRLIPGFWAPTAALWGFENRTCALRVI
                     GGSDKSQRVEYRIAAADINPYIALAAAIGSGLWGIERRAEPDAPVAGNAYAIDPPSER
                     RLPATLWDAAQRLRSSDAARGLFGAEFVDHYAATREWEEREARKAVTDWQLARYFEII
                     "
     gene            3500..4801
                     /gene="hslU"
                     /locus_tag="VF547_05000"
     CDS             3500..4801
                     /gene="hslU"
                     /locus_tag="VF547_05000"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX8262212.1"
                     /translation="MNDNLTPKAVVAALDEHIVGQTEAKRAVAVALRNRWRRQRLENG
                     LREEVTPKNILMIGPTGCGKTEISRRLAKLADAPFVKVEATRFTEVGYVGRDVEQIAR
                     DLVEEAVRLEKERRRLAVKDAAEKAAMERLLEALTGKGASEATRESFRQRFLEGHLNG
                     SEVELELAEAPNAPFEIPGMGGQVGMINLSEMMSKAMGQSPKKRRRLKVPEAWTRLVE
                     EEADKRLDPDDVNRVALADAEANGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIE
                     GTTVATKYGPMKTDHILFIASGAFHVAKPSDLLPELQGRLPIRVELKALTEEDFVRIL
                     SDTRASLTEQYRALIATEGVEVEFTDEGIRAIARIAAEVNGQVENIGARRLSTVLEKL
                     LEDVSFEAEERRGTRVVVDAPYVEAQLADIARNTDLSKYVL"
     gene            4969..5271
                     /locus_tag="VF547_05005"
     CDS             4969..5271
                     /locus_tag="VF547_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX8262213.1"
                     /translation="MRKFTLALCAATMAVPVIPATPAFANHNGYYHGKTWRDSQGRIR
                     CRRSNGTVGIIVGGAAGALVGRAVDGGRNRTVGTVVGAAAGALAGREIARNRRRCR"
     gene            5469..7088
                     /locus_tag="VF547_05010"
     CDS             5469..7088
                     /locus_tag="VF547_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262214.1"
                     /translation="MINSLSKLFGRRGSAGLALALALPGLAACQEASTGNEAGKAPGA
                     QAAAANPCGTFAPTRTSALPSNPGTNQVQIDCSAWQAFIALNWKADPAKRGYPDASAQ
                     WASFGTPGDTSPTVWESYWEASAVFGSATPLTGQWQTKLPAVKSLGRTSKFGALDLTD
                     IDQAGSGHHWLTSQRGEITYYEVLMNRDEFEFITTQNLTTAAGQLACVTQPGKPISDG
                     PPPPPDAPKRGGLNMPAGQQPGWDDTDCVGNVASYGDGVGAMEIKAAWAPLPADGSLN
                     YRYKTAVAEIRDPITRSMRRVTVGLVGLHIIRKRFPRLPWIWATFEHIDNTPDEAPNN
                     GFSPPKLPANPNQKPSPGYTYFNPNCNPQTDKAYKCVHNAPPTTRCLLGTNPATCQPF
                     NAPMQITRINPVDDVANSVTAYVWSLLPAQSVFNYYRLINVQWPQNGIGQPPQNIPPG
                     PGLRVPLYNGNPTPQGNAGGPGQIVANTTMESFQQKSNACMDCHVFASIHAATTPQGA
                     GGLRKIGKGATSGQPPYASDYSFIFASETRR"
     gene            7257..7547
                     /locus_tag="VF547_05015"
     CDS             7257..7547
                     /locus_tag="VF547_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262215.1"
                     /translation="MGTFLSAAGAAALGSVLGAVAAGKIRRAAAHDGASRALWAAGWV
                     AALAAAGALAVDYYADGAGLIALAAGAAGGAAWDGMVGARRWAAASAGERLE"
     gene            7544..8494
                     /locus_tag="VF547_05020"
     CDS             7544..8494
                     /locus_tag="VF547_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262216.1"
                     /translation="MIFDWQVAGAFAFGALIGWYVYYVNRYRTGDIQIGDVVTLIGAI
                     GGSAVLALFDRKTDLFGAYGVGLAVGFFGYFIVLAVLVKMSSNFDRDWFLDGRRKDPQ
                     EGYGYGTNTRPTIAPMAINVLGTPTPTPPPQVAAPGIQFIGVNPGEAAALPAPAEAPR
                     PLELPNAATGRVHTVCASVWAESGPTGPFRNSANGFVIEVAHRLGLTLSGTVDQMVAQ
                     AEARGWTRLAGDAEAREAAAQGRFVLAAVKADDYDPPRLHGQAAVVAADPRQARSGLP
                     RGYWGSDSPEVAEAGGMAAPLSRCFPADVRSRIVYLAREM"
     gene            8504..9238
                     /locus_tag="VF547_05025"
     CDS             8504..9238
                     /locus_tag="VF547_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262217.1"
                     /translation="MADQEIPEFCSTLEDTGFQRGVGGFQERAALLNAAFWGRGVRLR
                     VSFLEGAPELHNRVAGLARLWETQTGANFAFEFWINDGRDPRDADIRVAFRPDKGSNS
                     VLGRYARSVDRDKRTMNLGWMTMELDEDRARAVVLHEFGHALGLIHEHMSPAQPINWN
                     KERVREDMRRAHGWDDAKIDANMFAKYEAREVFSTPVDARSIMMYPIPPEWTTDGFSA
                     PFNPDLTPTDIALVRQAYGVRPAFGG"
     gene            9239..>9446
                     /locus_tag="VF547_05030"
     CDS             9239..>9446
                     /locus_tag="VF547_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262218.1"
                     /translation="MASAGPNDRAIVVGIGRYPRFGPNGTGPNDLLGPVADAREVADW
                     LVNTARATVTLITSDGDGSQNWTAT"
BASE COUNT         1516 a         3324 c         3226 g         1380 t
ORIGIN      
        1 ctccggatcg aggatgaccg cggccggcat catccgcggg tggaagatga tcttcttgac
       61 ggattcggcc tcgtccagga tcactccggc gcggccgacc tcggagccgg tgccggccgt
      121 cgtcgggacg gcgatcaccg gcgcgatccc ggccggatcg gcgcgggacc accaatcctc
      181 cttgtcctcg aagtcccaga gcggccgggc ctgacccgac atgaaggcga caaccttgcc
      241 gacgtcgagc gcgctgccgc cgccgaacgc gacgaccccg tcatgactgc cgccgcgata
      301 aaccgccaga cccgcctcga cgtcggcgcc gaccgggttc ggatggatgt cgcagaacaa
      361 tccggcgccg gtcgcggcga gcgcggcctg gaccatcggc agcttggcga gcgctggatc
      421 ggtgacgagc aggggccgct cgatcccgag ctcgcggcac gagtccccga gctccgcggc
      481 ccggccggcg ccgaagcgga tcgtggtcgg ataattccag ttcgcgcggg ccagggccat
      541 cagctgctcc gcaggtgcag ggactttggg cgggtaacgc tctcgaagcc gtggcggccg
      601 agcgaagcgc ctcggccgct gtccttcacc cccgtccagg ccagcagagg atcgagatag
      661 tcgcagcggt tggcgaagac ggtccccgcc tccacccgcg ccgccagccg ctcggcggcc
      721 tggaggtcgc gcgtccagat cgaggcggtg agcccgtagc ggctgtcgtt catcagccgt
      781 acggcctcct cgtcgttccg gaccgccatg atcccgatca ccggcccgaa cgtctcctcg
      841 cgcatcaccg ccatgccgtg gtcgacgtcc gtcagaacct gcggcatcag atagggcccc
      901 tcgccggagt ccgtcgggaa gagctggggc gggaggtgcc ccgtcgcgcc cgcctcgatc
      961 gcctggcgga cctgcgcgcg caccaggtcg gccgccccgc cgctgaccat cgggccgagg
     1021 ctggtttccc ggtccagcgg attgccgagc cggtattgcg acgtggcggc gacgaagctg
     1081 tcgaggaacg cctcccagac cgcctcgtgg acatagatgc gctcgacccc gcagcaggat
     1141 tggccgctgt tgaagaagct gccgtcgacc aggccgtcga ccgtgccggc cagctccgcg
     1201 tcctcgcgca cataggcggg atccttgccg ccgagctcga gcgtgagagt gatgaaggac
     1261 cccgccgccg cccgctcgac cgccttgccc gcctcgaccg agccggtgaa ggtgacgtgg
     1321 tcgaccgcgc ctgcgccgag cagccgcgcc gtctgttcgt ggctcaggaa taggttggcg
     1381 aacagcccct cgggcaggcc ggacctgccg agcgcctcgg cgaagcgctc gccggcgagc
     1441 ggcgtctggg aggcatgttt gaggatgacg gcgttgccgg cgagcagcgc cgggacgatc
     1501 gtgttggcgg cggtgaggaa gggatagttc cagggagcga tcaccagcac caggccggcc
     1561 gacacccgca ccaccgagcg tccggcgccg gccacgaacg gcgcgagcgc ggacggcgcg
     1621 aggtcgacca tcgcccgcac ccgctccgcg agcgagcgca actcgcctcc gtagcggacc
     1681 ggacggccca tctgccaggc cagctcgggg acgattgccg ggttgagggc ctccatcgcc
     1741 tcgagaaacc gaagcatcgt cgcgcagcgc tcgtcgagcg gcgtccgggg ccattcgcgc
     1801 tgcgcgcggc gagcggcgtc gagagccttg tcgacctcgg cgtccgtcgc catcggccgc
     1861 cgcgcatatt ctcggccgtt gacgggggag atgcagacga tctcgttcaa gtgtccgttc
     1921 gtcctccaac cgctcatcct gagcagggct gagcctggcg aagacccgta tcgaaggacc
     1981 gtccttcgat acgccgcttc gacttcgctc agcggctact caggatgagc gtatgtgtca
     2041 ggcaaaaaat cgcgccattc agatgatctc gaaatagcgg gcgagctgcc agtcggtcac
     2101 cgccttgcgg gcctcgcgct cctcccactc gcgcgtggcc gcataatggt cgacgaactc
     2161 ggccccgaac agcccgcgcg ccgcgtcgga cgagcgcagc cgctgcgccg cgtcccacaa
     2221 ggtggccgga agcctccgct cggacggcgg gtcgatcgca taggcgttgc cggcgacggg
     2281 cgcatcaggc tcggcgcggc gctcgatgcc ccacaggccc gatccgatcg ccgcggcgag
     2341 ggcgatgtag ggattgatgt cggcggcggc gatccggtac tcgacccgct ggctcttgtc
     2401 ggacccgccg atgacgcgca gcgcgcaggt gcggttctca aagccccaca gcgccgccgt
     2461 cggcgcccag aagccgggga tcagccttgt gtaggcgttg accgtcggcg ccaccatcgc
     2521 cagcagctcg ggcatcagcg cctgctggcc gccgacgaac cagcgcatct cctgcgacat
     2581 gccgagctcc gccccctcat cgtggaagcg cgacctgccg ctgccggcgt cgaccagcga
     2641 catgtggagg tggccggact ggcccggcca gtcgttcgac catttcgcca tgaacgtcgc
     2701 catccagccg cgccgctggc ataggatctt gacgatcgtc ttgaacaagg ccgccttgtc
     2761 cgccgcctcg agcgcgtcgt cgacccggat cgcgccctcg atcacgcccg gcccggtctc
     2821 ggtgtggagc ccctccagct cgatccgcat cgcctcgcag gtcgaccaca gctcggcgta
     2881 gaagtcggca tggaccccgg aacgaagcat cgaatagccg aaatggccgg gcgtgatcgg
     2941 cttcagctgc cgatagccct tgtcgcgcac cgattgcggg gtctcctcga acacgaagaa
     3001 ttcgaactcc gccgccgcct tgacgcggca acccatcccc gcggctcggt ccagcacccg
     3061 gcgaagcacg ccgcgcgggc agatcgcctc ggctgccccg gcaaactcgc cgaggatgag
     3121 cggcattccg ccttcgaacg gcatcgtgcg ctccgtatcg ggcagaagcc gcacctcggc
     3181 gtcgggatag gcggtgtgcc agccggtgaa ggcggcattg tcgtagagct ggtcgctcga
     3241 atcccagccg agcacgacgt cgcagaagcc gaagcccccg gcgagcgccg aggcgagctt
     3301 gtccggcgcc atgtacttgc ctcggaagat gccgtcgacg tcgaacacgc cgaccttcgc
     3361 gcaggcgggg tcgcgctcgc ggattcgctt caccaggtcg cccttcgcca tgcctgcgcc
     3421 tcatctctgc tcggcttgat tcccgtctgg gtcgcgccat ctaggctggc aagagcattt
     3481 gcagggcatt cggcgctaaa tgaacgacaa tctcactccc aaggcggtgg tcgccgccct
     3541 cgacgagcat atcgtcggcc agaccgaggc gaagcgcgcg gtcgcggtcg ccctgaggaa
     3601 ccgctggcgg cggcagcggc tcgagaacgg tctgcgggag gaggtgacgc ccaagaacat
     3661 cctgatgatc ggcccgaccg gctgcggcaa gaccgagatc agccggcgcc tcgccaagct
     3721 cgccgacgcg ccgttcgtca aggtcgaggc gaccaggttc accgaggtcg gctatgtcgg
     3781 ccgcgacgtc gagcagatcg cacgcgacct cgtcgaggag gcggtgcggc tggagaagga
     3841 gcgccgccgg ctggcggtca aggacgcggc cgagaaagcg gcgatggagc ggctgctcga
     3901 ggccctgacc ggcaagggcg ccagcgaggc gacccgcgag agcttccgcc agcgcttcct
     3961 cgaaggccat ctgaacggtt ccgaggtcga gctcgagctc gccgaggcgc ccaacgcgcc
     4021 gttcgagata ccgggcatgg gcgggcaggt cggaatgatc aacctctccg agatgatgtc
     4081 gaaggcgatg ggccagtcgc cgaagaagcg gcgccgcctc aaggtgccgg aggcctggac
     4141 tcggctcgtc gaggaggagg cggacaagag gctcgacccg gacgacgtca accgagtcgc
     4201 cctcgccgac gccgaggcga acggcatcgt cttcctcgac gagatcgaca agatcgccgt
     4261 ctcggacgtg cgcggcggct cggtctcgcg cgagggcgtg cagcgggacc tgctgccgct
     4321 gatcgagggg acgaccgtcg ccaccaaata cgggccgatg aagaccgacc atatcctgtt
     4381 catcgcctcg ggcgcgttcc acgtcgccaa gccgtcggac cttcttcccg agctccaggg
     4441 ccggctgccg atccgggtcg agctgaaggc tctgaccgag gaggatttcg tccggatcct
     4501 gtccgacacc cgggccagcc tcaccgagca gtaccgagcg ctgatcgcca ccgagggcgt
     4561 ggaggtggag ttcacggacg agggcatacg cgccatcgcc cggatcgccg ccgaggtgaa
     4621 cgggcaggtg gagaatatcg gcgcccgccg gctttcgacg gtgctcgaga agctgctcga
     4681 ggacgtcagc ttcgaggcgg aggagcgccg cggcacccga gtcgtcgtcg atgcgcccta
     4741 cgtcgaggcc cagctcgccg acatcgcccg caacacggac ctgtccaaat acgtgctctg
     4801 agccgctagc cgttcgcggc gccgaaagcg gaacggcgga cggttctcca cccgcgccca
     4861 gccgagcggg aatcgccgat ttcggggatc gttgtctcag gttaatgaaa ctggcggcaa
     4921 ccccgccgcg ttcatggttc gcttcttcac ggggaaagga cccaagtcat gcgtaaattc
     4981 accctcgcgc tctgcgccgc caccatggcc gtgccggtga tcccggccac gcccgccttc
     5041 gccaatcaca acggctatta tcacggcaag acctggcgcg attcgcaggg ccggatccgc
     5101 tgccgccgtt ccaacggaac ggtcggaatc atcgtcggcg gcgccgccgg agcgctggtc
     5161 ggccgagcgg tcgacggcgg ccgcaaccgc accgtcggca ccgtcgtggg cgccgccgcc
     5221 ggcgctctgg ccggccgcga gatcgctcgc aaccgccggc gctgccgcta ggctcgcgca
     5281 cgcctttcaa cggcgaacgg ggcgctccgc gaggggcgcc ccgtttcgat tccgggaaga
     5341 accgtttcgg acccaacatt gatttgcacc gattcgggaa acctgcttca acggcctccg
     5401 gggggcgtcg cttccactgt gcgcacagcg taacccggct tcggccgtgg aaggggggag
     5461 aggcgaaaat gatcaattcg ttgtcgaagc tgttcggccg ccgcggttcc gcggggctcg
     5521 cgctggcctt ggcgctgccc gggctggcgg cctgccagga ggcttcgacc ggcaatgagg
     5581 ccggcaaggc ccctggggcc caggctgcag cggccaatcc ctgcggcacc ttcgccccga
     5641 cccggacgtc ggcgcttccc tcgaaccccg gcaccaacca ggtccagatc gactgcagcg
     5701 cctggcaggc cttcatcgcg ctgaactgga aggccgatcc ggccaagcgc ggctatccgg
     5761 atgcctcggc gcaatgggcc agcttcggca cgccggggga caccagcccg acggtctggg
     5821 aaagctattg ggaagcgtcg gccgtcttcg gcagcgccac tccgctcacc ggccagtggc
     5881 agaccaagct tccggcggtg aaatcgctcg gccggacgtc gaagttcggc gcgctcgacc
     5941 tcaccgacat cgaccaggcc ggcagcggcc accactggct gaccagccag cgcggcgaga
     6001 tcacctatta cgaagtgctg atgaaccgcg acgagttcga gttcatcacc acccagaacc
     6061 tgaccaccgc ggccggtcag ctcgcctgcg tcacccagcc gggcaagccg atctccgacg
     6121 ggccgccgcc gccccctgac gcgcccaagc gcggcggcct caacatgccg gcggggcagc
     6181 agccgggctg ggacgatacc gattgcgtcg gcaacgtcgc cagctatggc gacggcgttg
     6241 gagcgatgga gatcaaggcc gcctgggctc cgctcccggc cgacgggtcg ctcaactatc
     6301 gctacaagac ggccgtggcc gagatcaggg acccgatcac gcgctcgatg cgcagggtga
     6361 cggtcggcct cgtcggcctc cacatcatcc gcaagcgctt cccgcggctg ccctggatct
     6421 gggcgacgtt cgagcatatc gacaacacgc cggacgaagc gccgaacaac ggcttctcgc
     6481 cgcccaagct gccggccaat ccgaaccaga agccgtcgcc gggctacacc tacttcaatc
     6541 ccaactgcaa tccgcagacg gacaaggcct ataaatgcgt gcacaacgcg ccgccgacga
     6601 cgcgttgcct gctcggcacc aaccccgcca cctgccagcc gttcaacgcg ccgatgcaga
     6661 tcacccgaat caacccggtc gacgacgtcg ccaacagcgt caccgcctat gtgtggagcc
     6721 tgctgccggc ccagtcggtg ttcaattact accggctgat caacgtccag tggcctcaga
     6781 acggaatcgg ccagccgccg cagaacatcc cgccgggacc gggcctcagg gtccccctgt
     6841 acaacggcaa tccgactccg cagggcaatg ccggcggtcc gggccagatc gtcgccaaca
     6901 cgacgatgga gtccttccag cagaaatcga acgcctgcat ggactgccac gtcttcgcct
     6961 cgatccacgc cgcgacgact ccgcagggcg ccggcggcct gcgcaagatc ggcaagggcg
     7021 cgacgtccgg ccagccgccc tacgcctcgg attattcgtt catcttcgcc agcgagacga
     7081 ggagatgatc gcaggctgac gtcggccggc gttgcgccgg ccggcgcggt cgccgaacgg
     7141 ggcgcgttgc caatatcccg gggccgatgg cacaatcccg ccgccggagc cgagtcgtcg
     7201 gccggccgac cggcgcagga gcgagtccgc attcgaagcg ggctcaggag gttgtcgtgg
     7261 ggaccttttt gagcgcggcc ggagcggcgg ccctgggatc cgtgctcggc gcggtcgcgg
     7321 cgggcaagat ccggcgggcg gccgcgcatg acggtgcgtc gcgggccttg tgggcggccg
     7381 ggtgggtcgc ggccctcgcc gccgcgggcg cgctcgccgt cgactattat gcggacgggg
     7441 caggcctgat cgccttggcc gcgggcgccg ccggtggggc ggcctgggat gggatggtcg
     7501 gtgcgcggcg ctgggcggcc gcgtcggcag gggagagatt ggaatgatct tcgactggca
     7561 ggtggcgggt gccttcgcct tcggcgcgct gatcgggtgg tatgtctatt acgtcaaccg
     7621 ctaccggacg ggcgacatcc agatcggcga cgtcgtcacc ctgatcggcg cgatcggcgg
     7681 cagcgccgtg ctcgcgctgt tcgatcgcaa gacggacctt ttcggagcct atggagtcgg
     7741 cctggcggtg ggcttcttcg gctatttcat cgtcctcgcc gtcctcgtga agatgtcgtc
     7801 caacttcgac cgggactggt tcctcgacgg ccggcgcaag gacccgcaag agggctatgg
     7861 ctacggcacc aacacccgtc cgacgatcgc tccgatggcg atcaacgtcc tggggactcc
     7921 gacgccgact ccgccgcccc aggtcgcagc gccggggatc cagttcatcg gcgtcaatcc
     7981 cggcgaggcc gccgctttgc ccgcgcccgc cgaagcgccc cgtccgctcg agctgcccaa
     8041 tgcggccacc ggccgagtcc ataccgtctg cgcctccgtg tgggcggaat ccgggccgac
     8101 gggcccgttc cgcaattcgg cgaacggctt cgtgatcgag gtggcgcatc ggctcggcct
     8161 gaccctctcg ggcaccgtcg accagatggt cgctcaggcc gaagcgcgcg gctggactcg
     8221 cctggccgga gatgcggagg cgcgggaggc cgcggcgcag ggacggttcg tgctcgccgc
     8281 ggtcaaggcc gacgattacg acccgccccg cctccacggc caggccgccg tcgtcgccgc
     8341 cgatccgcgt caggcgagga gcggacttcc gcgaggctat tggggcagcg actcgccgga
     8401 ggtggcggag gcgggcggaa tggcggcgcc gctcagccgg tgcttcccgg cggacgtcag
     8461 gagccgcatc gtctatcttg cccgggagat gtgagggaca gccatggcgg atcaagagat
     8521 acccgaattc tgctcgacgc tcgaggacac cggcttccag cggggcgtgg gcggcttcca
     8581 ggagcgtgca gccttgctca acgcagcctt ctgggggcgg ggcgtgcggc ttcgagtctc
     8641 attcctcgag ggcgcgccgg agcttcacaa ccgggtcgcc ggcctcgcca ggctgtggga
     8701 gacgcagacg ggagccaatt tcgcgttcga attctggatc aacgacgggc gcgacccgcg
     8761 cgacgcggac atccgcgtgg ccttccggcc cgacaagggc tcgaactcgg tgctcggccg
     8821 ttacgcgcgg tcggtcgacc gcgacaagcg gacgatgaat ctcggctgga tgacgatgga
     8881 gctggacgag gaccgcgcgc gggcggtcgt cctccacgag ttcggccatg ccctcggcct
     8941 gatccacgag cacatgagcc cggcccagcc gatcaactgg aacaaggagc gggtgcgcga
     9001 ggacatgcgc cgggcgcacg gctgggacga cgccaagatc gacgccaaca tgttcgcgaa
     9061 atacgaggct cgcgaggtct tctcgactcc ggtcgatgcc cggtcgatca tgatgtaccc
     9121 catccctccg gaatggacga cggacggctt ctcggcgccg ttcaatcccg acctcacccc
     9181 gaccgacata gccctcgtcc gccaggccta tggcgttcgg ccggccttcg gcggctagat
     9241 ggcgtcggcc gggcctaacg accgggcgat cgtcgtcggc atcgggcgct atccgcgctt
     9301 cggtcccaac gggaccggcc ccaacgacct gctgggcccg gtcgccgacg cccgggaggt
     9361 ggccgactgg ctggtcaaca ccgcgcgcgc gaccgtcacc ctgatcacct cggatggcga
     9421 cggcagccag aactggaccg cgacgg
//
LOCUS       DASVQZ010000182         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_38349, whole genome shotgun sequence.
ACCESSION   DASVQZ010000182 DASVQZ010000000
VERSION     DASVQZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38349"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /gene="dgcA"
                     /locus_tag="VF547_05035"
     CDS             complement(<1..983)
                     /gene="dgcA"
                     /locus_tag="VF547_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HEX8262219.1"
                     /translation="MSRTLRAQHDRFALIRPFRIARGVKTFADVITVTLSEGAVCGRG
                     EGVPYPRYGESVETALAAVDRARAEIEAGAGREALLSLLPPGAARNAIDCALWDLEAR
                     RSGGDVAEMLGIPPPGPLVSALTIVIDTPQAMAAAAIESGGAPLLKVKVDADDPAARI
                     RAVRAAAPEAALIVDPNESWDRRRVEAMQPVLLEAAVALLEQPVAAGEDAWLEDFAPA
                     VPICADESVHVAADLDLVARRYQAVNVKLDKAGGLTAAMELALAARARGLALMTGCMV
                     SSSLSIAPALHVAGLSDFVDLDGPLWLGEDRPGGVSVGRGMLAPPESGFWGR"
     gene            complement(941..1975)
                     /gene="dgcN"
                     /locus_tag="VF547_05040"
     CDS             complement(941..1975)
                     /gene="dgcN"
                     /locus_tag="VF547_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN"
                     /protein_id="HEX8262220.1"
                     /translation="MIPGPYLLYLGRSADPMAIKTSRGLATFRRGDCVGEYRHDDCPL
                     TLGLPRMTMAEGVAAGARTLVLGIAGAGGRLGNDLIDDAALALAAGMNVAAGLHQRLR
                     DEPRLRALAHDGGLQLFDVREPPADLAVGNGYPRPGRRLLTVGTDCSVGKMYAALALT
                     AALRERGCAADFRATGQTGILIAGKGVPVDAVVADFISGAIERLAPAREDSGWDVIEG
                     QGSLFHPSFAGVSMGLLHGAQPEALVLCHVPGRSHMRGLPGRALPDLAECLQANLEAA
                     RLTSPGVAAVGVCLNTAGTPPDEARRLCGRTADRLGLPCTDPIAFGVDSIADRLACPE
                     PSARSTIASR"
     gene            complement(2044..2928)
                     /locus_tag="VF547_05045"
     CDS             complement(2044..2928)
                     /locus_tag="VF547_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8262221.1"
                     /translation="MNLRHIEIFHAVYLNGSVSAAARALNVSQPSVSKMLRHAESLLG
                     FQLFQRTNGGLVPTEDAHVLFTEASEIQDRVHALREAGRQLKRGEGGMLRISALPSLA
                     LQAIPVAVSRFRRTHEKVKFDLQTVHHDDLLRKLYERETDVAIAFQVPSAAPIGHRRL
                     GEGELVVLYQERDMPDAPPRVELGQLRGRPFISLAASGPIGQLFTQELHRLDVELDDI
                     VSARTFYIATALVRQGVGMTVVDSFTAQASLAPGLSVRPLEPKLTFDIHAMYLTNRPP
                     NALAMDFLKTLARVIEAP"
     gene            complement(3166..>4376)
                     /locus_tag="VF547_05050"
     CDS             complement(3166..>4376)
                     /locus_tag="VF547_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262222.1"
                     /translation="PSLSAGKLLAGREWRTRLIALLVANPVSERFSAAEDDKQIRGGQ
                     WVASFDSVVPQAEWNALVNADPPKAEDLKNQTSFAAYAERLVQAAMTQDRMPESHRQR
                     IARIADRTGGREAFNALPADFRRELLVAGKVDVVRLDQKIGRAGAVIVTAQGQRILFD
                     SPVSQADWNRAINLMPPRTVTTGADLIAHVQRIASRIIVDEKFRASLGTATAWYNGYV
                     KALPSYGRQAITCGGRVLANVTVSGNRTDGFTQGVHIGTSGLRRFANMAPTPYLAGVV
                     TVEGNNLFLRLPGEDVYAACGLFVGNAETIRVERNTLAWSATRGSSKRTFAQGIRIWG
                     HLGPFLAIVENRVDTAPLGIRVKIMERLTEQKIAKLMWLASDNHVPSAAAGLTLRVPR
                     FMEKRNNKPA"
BASE COUNT          656 a         1505 c         1595 g          620 t
ORIGIN      
        1 cgtccccaga agcctgactc gggcggcgcg agcattcctc gaccgacgct cacgcccccc
       61 ggcctatcct ccccgagcca caggggaccg tcaaggtcga cgaagtccga gaggccggcg
      121 acgtgcagcg ccggcgcgat cgacagcgac gagctgacca tgcagccggt catcagggcg
      181 aggccgcgcg ccctcgcggc gagcgccagc tccatcgcgg cggtgaggcc gccggccttg
      241 tcgagcttga cgttgaccgc ctggtagcgg cgcgcgacga ggtcgagatc ggcggcgacg
      301 tggaccgact cgtcggcgca gatcggcacg gccggagcga agtcctccag ccaggcgtcc
      361 tcgccggcag ccacgggctg ctcgagcagg gcgacggcag cctcgaggag gacgggctgc
      421 atcgcctcca cccggcggcg gtcccagctc tcgttcggat cgacgatcag cgccgcctcg
      481 ggcgcggcgg cgcgcacggc gcggatgcgg gcggccggat cgtcggcgtc gaccttgacc
      541 ttcagcaagg gcgcaccgcc ggattcgatc gccgcggcgg ccatcgcctg cggcgtgtcg
      601 atgacgatgg tgagagcgct gaccagcggt cccggcggag ggatcccgag catctccgcc
      661 acgtcgccgc ccgatcgccg ggcctccagg tcccagagcg cgcagtcgat ggcattgcgg
      721 gcggcgccgg gcggaagcag cgaaagcagc gcctcccggc cggcgccggc ctcgatctcg
      781 gcgcgggccc ggtcgaccgc ggccagcgcg gtctcgacgc tctccccgta gcgcggatag
      841 ggaacgccct cgccgcggcc gcagacggcc ccttcgctca acgtcacggt gatcacgtcg
      901 gcgaacgtct tgacgccgcg cgcgatccgg aacggccgga tcagcgcgaa gcgatcgtgc
      961 tgcgcgcgga gggttctgga catgcgagcc ggtcggcgat ggagtcgacg ccgaaggcga
     1021 tcgggtcggt gcagggcagg ccgagccggt cggccgtccg gccgcacagc cgccgcgcct
     1081 cgtcgggcgg cgtccccgcc gtgttcaggc agacgccgac ggctgcgacc ccggggctgg
     1141 tcagccgagc cgcctcgagg ttggcctgga ggcattcggc gagatcggga agagcgcgcc
     1201 cgggaaggcc gcgcatgtgg ctgcggccgg ggacgtggca caggacgagc gcctcgggct
     1261 gggcgccgtg cagaaggccc atcgacactc ccgcgaacga gggatggaac agcgagccct
     1321 ggccctcgat gacgtcccag cccgaatcct cgcgggcggg cgcgagccgc tcgatcgccc
     1381 cggagatgaa gtcggcgacg accgcgtcga ccggaacgcc cttgccggcg atcaggatgc
     1441 cggtctggcc ggtcgcccgg aaatcggcgg cgcagccgcg ctcgcggagc gccgcggtga
     1501 gcgcgagggc ggcgtacatc ttgcccaccg agcaatcggt gccgacggtc agcagccgcc
     1561 gccccggccg gggatagccg ttgccgacgg cgaggtccgc cggcggctcg cgcacgtcga
     1621 acagttgcag gccgccgtca tgcgcgagcg cccgcagccg gggctcgtcg cgcagccgct
     1681 ggtggagacc ggcggcgacg ttcatcccgg ccgcgagcgc cagggccgcg tcgtcgatca
     1741 ggtcgttgcc gaggcgaccg cccgcccccg cgatcccgag caccagtgtg cgggcgccgg
     1801 ctgccacgcc ttcggccatg gtcatccggg gaaggccgag cgtgagcggg caatcgtcgt
     1861 gacgatattc gccgacgcag tcgccgcgac ggaaggtggc gagcccccgg gaggtcttga
     1921 tcgccatcgg gtccgcgctg cgcccgagat agagaagata ggggcccgga atcatggccc
     1981 gaacctatcg ccgagccggg ctcacgggcg catagcgagt gggccgctcg ccataatctg
     2041 ccgttatggc gcctcgatca ccctcgcgag cgtcttcagg aagtccatgg cgagcgcgtt
     2101 gggcggccgg ttggtcaggt acatggcgtg gatgtcgaag gtcagcttcg gctcgagcgg
     2161 ccggaccgac aggcccggcg ccagcgacgc ctgggcggtg aagctgtcga ccaccgtcat
     2221 cccgacgccc tggcggacga gcgcggtggc gatgtagaag gtgcgcgccg agacgatgtc
     2281 gtcgagctcg acgtcgagcc ggtgaagctc ctgggtgaac agctggccga tcgggccgct
     2341 ggcggcgagg ctgatgaacg gccgcccgcg cagctgcccg agctcgaccc gcggcggagc
     2401 gtcgggcatg tcccgctcct ggtacaggac gacgagctcg ccctcgccga gccggcggtg
     2461 gccgatcggc gccgcggaag gcacctggaa ggcgatcgcg acgtcggtct cgcgctcgta
     2521 gagcttgcgc aggagatcgt cgtggtggac ggtctggagg tcgaacttca ccttctcatg
     2581 ggtccgccgg aagcgcgaca ccgccaccgg gatcgcctgg agcgcgagcg agggcaaggc
     2641 ggagatgcgc agcatcccgc cttcgccgcg cttcagctgc cgacccgcct cgcgcagggc
     2701 gtggacccgg tcctggatct cgctggcctc ggtgaacagg acgtgcgcat cctcggtagg
     2761 gacgaggccg ccgttggtcc gctggaacag ctggaagccg agcagcgatt cggcgtggcg
     2821 cagcatcttc gagaccgacg gctgcgacac gttgagcgcg cgggcggcgg cgctgaccga
     2881 cccgttgagg tagacggcgt ggaagatttc gatgtgacgc agattcatgc gtcggcgcct
     2941 atcgacatgc ggccaagcta gaacagtttc cggcggaggg gaaatgcgcc ggttcgcggc
     3001 gccggcgcgg ctccagaaat gggtaggggc ggaccatctt tgtgccccgc cccatgctcc
     3061 aaaagggact cgaaccctat ggcaccaaag ttcagctgcc attgttaatc gaagcggcgc
     3121 caccttacac cgcccgcgcc cccgcggcaa gcacggcgga tggcatcacg ccggcttgtt
     3181 gttgcgcttc tccatgaagc gggggacccg cagggtcagg ccggccgcgg cactcgggac
     3241 atgattgtcg gacgccagcc acatcagctt cgcgatcttc tgctcggtca gccgctccat
     3301 gatcttcacc ctgatcccga gcggcgccgt gtcgacccgg ttctcgacga tcgcgaggaa
     3361 cggcccgaga tggccccata tccttatgcc ctgggcgaag gtcctcttgc tcgagccgcg
     3421 cgtggccgac caggcgaggg tgttgcgctc gacccggatc gtctccgcat tgccgacgaa
     3481 caacccgcag gccgcgtaga cgtcctcgcc cggcaagcgc aggaacagat tgttgccctc
     3541 caccgtgacg accccagcga gatagggagt gggtgccata ttggcgaatc gtctcagccc
     3601 gctggtaccg atatggacgc cctgggtgaa tccgtcggtg cggttgccgc tgaccgtcac
     3661 gttcgccagc acccggccgc cgcaggtgat cgcctggcgg ccgtagctgg ggagagcctt
     3721 cacatagccg ttataccagg cggtcgcggt gccgaggctg gcgcggaact tctcgtcgac
     3781 gatgatcctc gaggcgatgc gctggacatg ggcgatcagg tcggcgcccg tggtgacggt
     3841 ccgcggcggc atcaggttga tggcccggtt ccagtcggcc tggctgaccg gcgaatcgaa
     3901 caggatgcgc tggccctggg cggtgacgat gaccgccccg gcccgaccga tcttctggtc
     3961 gagccgaacc acgtccacct tgcccgcgac cagcagctcg cgcctgaaat cggcggggag
     4021 ggcgttgaac gcctccctgc cgccggtgcg atcggcgatg cgggcgatcc tctgccggtg
     4081 cgactccggc atccggtcct gggtcatcgc cgcctggacg agccgctcgg cataggcggc
     4141 gaagctggtc tggttcttga ggtcctcggc cttgggcgga tcggcgttga ccagggcgtt
     4201 ccattcggcc tggggcacga cggaatcgaa gctggccacc cactgcccgc cgcggatctg
     4261 cttgtcgtcc tcggcggcgc tgaagcgttc gctcaccgga ttggcgacga gcagagcgat
     4321 cagccgcgtg cgccactcgc gccccgccag cagcttgccg gcggacagcg atggcg
//
LOCUS       DASVQZ010000183         6268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_907072, whole genome shotgun sequence.
ACCESSION   DASVQZ010000183 DASVQZ010000000
VERSION     DASVQZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907072"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="queA"
                     /locus_tag="VF547_05055"
     CDS             complement(<1..947)
                     /gene="queA"
                     /locus_tag="VF547_05055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX8262223.1"
                     /translation="MRVDAFDFTLPPDRIALRPASPRDGARLLAVLGGRLEDRSVRDL
                     PELLRRGDTLVFNDTRVIPAQLEGRRGEARIGATLHKREGPRAWRAFVRNAKRVRDGD
                     RIDFGAGVAATAGDRGEDGSLRLTFEGEEPVEALLGRAGRMPLPPYIAGRREADARDR
                     DDYQTMFAREAGAVAAPTAALHFTPRLMEALAAAGIGHETLTLHVGAGTFLPVKADDT
                     ADHRMHSEWGRIEGATAARLNAARARGGRIIAVGTTSLRLLESAADGRGGVRAFEGDT
                     AIFITPGHDFRAVDGLVTNFHLPRSTLFMLVCALMGVET"
     gene            complement(966..1724)
                     /locus_tag="VF547_05060"
     CDS             complement(966..1724)
                     /locus_tag="VF547_05060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8262224.1"
                     /translation="MRMTTLLALLLGLFLSGSALAQGLTAPPAAPPLDPENTLLLDLS
                     SGGRVAIQLRPDVAPNHVARIKQLARKGFYDGLTFHRVIEGFMAQGGDPKGDGSGGSD
                     LPDLDAEFNGLPHVRGAIAAARTGAPDDAPPEAVKRAENSANSQFYIMLSPRLALDGR
                     YTVFGRVVSGMNFVDAIEKGEPPAVPTRILQASIAADNKPPPSPGQILTTAAPAPAPA
                     AGSGGGRVAGPAMPPPPSIRLPEAKPKPPRKKTQ"
     gene            complement(1790..2290)
                     /gene="coaD"
                     /locus_tag="VF547_05065"
     CDS             complement(1790..2290)
                     /gene="coaD"
                     /locus_tag="VF547_05065"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX8262225.1"
                     /translation="MRTGVYPGTFDPVTLGHMDIIRRGAHLVDRLVIGVTTNPSKSPM
                     FSVEERMAMVRREIAGIEGDIEVVSFDSLLMDFAEREGASLILRGLRAVADFEYEYQM
                     AGMNQQLNDRIETVFLMADVSLQPIASRLVKEIALYGGDIAKFVPPAVARDVARRVEA
                     VGRKGT"
     gene            complement(2305..3201)
                     /locus_tag="VF547_05070"
     CDS             complement(2305..3201)
                     /locus_tag="VF547_05070"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX8262226.1"
                     /translation="MADASLLPPALDQVRAEIDAFLSELLAVPPDPRARLYEAMRYAA
                     MGGGKRLRPLLVLASAALFHTARERAVRVGAAVECLHVYSLIHDDLPAMDDDDLRRGK
                     PTLHKAYGEATAILAGDALHALAFEIVADEETHEDPFVRAELVAELARAAGPAGMAGG
                     QMMDLAAAEEELDLAAVTRLQQLKTGALIGFCLEAGAIMGRVPPDKRTRLRGYARDVG
                     LAFQIADDLLDEEGHEEKTGKKVGKDRAKGKETFVTLLGRERARRQAELLVRQAVDYL
                     KSYGAEADLLRAIARFAVERDH"
     gene            complement(3211..3459)
                     /locus_tag="VF547_05075"
     CDS             complement(3211..3459)
                     /locus_tag="VF547_05075"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX8262227.1"
                     /translation="MPDPPEAISELSFEAALKRLEEIVRRLESGEAPLDESIELYGEG
                     DRLRQQCEARLQAAQARIEKIQFGRDGQPAGTAPFDPD"
     gene            complement(3499..4605)
                     /locus_tag="VF547_05080"
     CDS             complement(3499..4605)
                     /locus_tag="VF547_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8262228.1"
                     /translation="MTSSRAEPSLEPASPDVSLTDPDEAYSHKGLTYPLGRRGPGAGE
                     LIALADGVGWARLPIPGSLKHINVWVLDDGEGVALVDTGLDIAPCRDAWEALFAGPLA
                     GRRVTRVICTHFHPDHIGLAGRLTERWGVALWMTRGEWLYGRMLASDVRDAPPPEAIA
                     HWRAAGWEEGRIEAEAAKGWGRFASVVSPIPVGYVRMQEGDTLRAGGREWRVLIGSGH
                     CPEHACLVDEAGGLMIAGDQVLPRITSNVSVGLSEPEADPLGEWLDSIAKLRGLPDSL
                     LVLPSHGEPFTGLHARLDALARGHRERLDALHARLDEPRRAVDCFGTLFARQIDDGMY
                     GLATGEALAHLRRLEVEGRVVREALDGVHWYRAA"
     gene            complement(4586..5278)
                     /locus_tag="VF547_05085"
     CDS             complement(4586..5278)
                     /locus_tag="VF547_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HEX8262229.1"
                     /translation="MAQPLMPHATASWLVDNTSLTFEQIAEFCGLHILEVQAIADDTA
                     ATKLTGRDPIRAGELNQEEIDRGQADAGYRLRIQKEPDQTRRTKGPRYTPVSKRQDKP
                     DGIAWIIRNHPEISDGQIGKLIGTTRSTIAAIRDRSHWNIANIQPKDPVTLGLCSQRE
                     LDAIVAKAAKAAGIEPAPQDHRLAGDREALIEELRRERDTAAREAEAAHAAEEAGAAP
                     PREPTADDLFKS"
     gene            5255..5887
                     /locus_tag="VF547_05090"
     CDS             5255..5887
                     /locus_tag="VF547_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262230.1"
                     /translation="MGHERLGHGESPETRRAAPSRSGRHISSDLVQPPREGKKNAAIP
                     PHSHLDSAPNQVELGLGKRRGTFMKFVTLGALIAAHIACAAQAAELPREPSTEATRVG
                     TFAGARMRVPLGATKEKPNAGLAFTATQRTGDTGTLRFSKGMELGFAGRDKVQLSLAG
                     RHVSQLRQGAEGPNGRKHGFSTTGWVILGASAVIIIGGVLIFDYLGDQSE"
     gene            5896..6225
                     /locus_tag="VF547_05095"
     CDS             5896..6225
                     /locus_tag="VF547_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262231.1"
                     /translation="MKMKMAMIAALVAAQLPAAGYAAQATELPREESAAQPRAGEDAE
                     GVPADAQAPAPAAEKTPVGAPALAAEEDEPKKGKSTGDKVLTGAAVVLGVAAVAVAGL
                     LVAIFVN"
BASE COUNT          865 a         2197 c         2279 g          927 t
ORIGIN      
        1 atcgtctcga cgcccatcag cgcgcagacc agcatgaaca gggtcgagcg cggcaggtgg
       61 aagttggtga ccaggccgtc gaccgcccgg aagtcgtgcc ccggggtgat gaagatggcc
      121 gtgtcgccct cgaaggcgcg cacgccaccg cgcccgtcgg cggcgctttc gaggaggcgc
      181 agcgaagtcg tgccgacggc gatgattcgg ccgccccgag cgcgcgcggc gttgagccgg
      241 gccgccgtcg ccccctcgat ccggccccat tcgctgtgca tccggtggtc ggcggtgtcg
      301 tccgccttca ccggcaggaa ggtgccggcg ccgacatgga gggtgagggt ctcgtggccg
      361 atcccggccg ccgccagcgc ctccatcagc cgaggcgtga aatggagggc cgcggtcggc
      421 gccgcgaccg ccccggcctc gcgggcgaac atcgtctgat agtcgtcccg gtcgcgcgcg
      481 tcggcctccc ggcggccggc gatatagggc ggcagcggca tccggccggc ccgcccgagc
      541 agggcctcga ccggctcctc gccctcgaag gtgaggcgca ggctgccgtc ctccccgcgg
      601 tcgccggcgg tcgcggcgac cccggcgccg aagtcgatgc ggtcgccgtc gcggacccgc
      661 ttggcgttgc ggacgaaggc gcgccaggcg cgcggcccct cgcgcttgtg cagggtggcg
      721 ccgatccgag cctcgccgcg gcgcccttcg agctgggcgg ggatgacccg cgtgtcgttg
      781 aacaccagag tgtcccctcg ccgcagcagc tccggcagat cgcgcacgga ccggtcctcg
      841 agcctgccgc cgagcacggc gagcagccgg gcgccgtcgc gcgggctcgc cggcctcagc
      901 gcgatccggt ccggcggcag agtgaagtcg aaggcgtcga cccgcacctt cggcttacct
      961 tcggcttact gagtcttctt ccgcggcggc ttcggcttcg cctcggggag gcggatcgag
     1021 ggcggcggcg gcatcgccgg gccggcgacc cggccgccgc cggatcccgc ggccggagcc
     1081 ggggcgggcg cggccgtcgt caggatctgg ccgggcgacg gcggcggctt gttgtcggcc
     1141 gcgatcgagg cctggaggat ccgggtcggc accgccggtg gctcgccctt ctcgatcgcg
     1201 tcgacgaagt tcatgccgct gacgacccgg ccgaacacgg tgtaacggcc atccagcgcg
     1261 agcctcggcg acagcatgat gtagaactgg ctgttggcgc tgttctccgc ccgcttgacc
     1321 gcctcggggg gcgcgtcgtc cggcgctccg gtgcgggcgg cggcgatggc gccgcggaca
     1381 tggggcaggc cgttgaactc ggcgtcgagg tcgggaaggt cggagccgcc gctgccgtcg
     1441 cccttcggat cgccgccctg ggccatgaag ccctcgatga cccgatggaa ggtgaggccg
     1501 tcgtagaagc ccttgcgggc gagctgcttg atccgggcga cgtggttggg cgccacgtcc
     1561 ggccgaagct ggatcgccac ccgtccgccg ctcgacagat cgagcagcag cgtgttctcc
     1621 ggatcgagcg gcggagccgc cggcggcgcc gtcaggccct gcgccagtgc cgagccggag
     1681 aggaacaggc ccagcaacag ggccagcaaa gtcgtcatac gcatgtttct ggaattcccc
     1741 ggataattcc gttcccgtcg ggcgatgatg cgcggcgggc gcggaaggat caagtgcctt
     1801 tgcggccgac ggcctcgacc cggcgggcga cgtcgcgggc gacggccgga ggcacgaact
     1861 tggcgatgtc gccgccgtag agggcgatct ccttgaccag ccgcgaggcg atcggctgga
     1921 gcgagacgtc ggccatcagg aagacggtct cgatccggtc gttcagctgc tggttcatcc
     1981 cggccatctg atactcgtac tcgaaatcgg cgacggcgcg cagtccgcgc aggatcagcg
     2041 acgcgccctc ccgctcggcg aagtccatca gcagcgaatc gaacgagacg acctcgatgt
     2101 cgccctcgat cccggcgatc tcccgccgga ccatggccat ccgctcctcg accgagaaca
     2161 tcggcgactt ggacgggttg gtcgtgactc cgatgaccag ccggtcgacg aggtgggcgc
     2221 cgcggcggat gatgtccatg tggccgagcg tgaccggatc gaacgtgccg ggatagacgc
     2281 ctgtcctcat agtcttccct tccgtcaatg gtcgcgctcc accgcgaagc gcgcgatcgc
     2341 ccgaagcagg tccgcctccg ccccgtagct cttcagatag tcgaccgcct ggcggaccag
     2401 cagctcggcc tggcgccggg cgcgctcgcg gccgagcagg gtgacgaagg tttccttgcc
     2461 cttggcgcgg tccttgccga ccttcttgcc ggtcttctcc tcatggccct cctcgtcgag
     2521 caggtcgtcg gcgatctgga aggcgaggcc gacgtcccgg gcatagccgc gcagccgagt
     2581 ccgcttgtcg ggcggcaccc ggcccatgat cgcgcccgcc tcgaggcaga agccgatcag
     2641 cgcgccggtc ttcagttgct ggaggcgggt gacggcggcg agatcgagct cctcctcggc
     2701 cgcggccagg tccatcatct gcccgccggc catcccagcc ggccccgccg cccgcgccag
     2761 ctccgcgacc agctcggcgc ggacgaacgg atcctcgtgg gtctcctcgt cggcgacgat
     2821 ctcgaaggcg agagcgtgga gcgcgtcccc ggcaaggatc gccgtcgcct cgccataggc
     2881 cttgtgcagg gtgggcttgc ccctgcgcag gtcgtcgtcg tccatcgccg gcaggtcgtc
     2941 gtggatcagc gaatagacgt gcaggcattc gaccgccgcg ccgacccgga ccgcgcgctc
     3001 gcgggccgtg tggaacagcg ccgccgaggc caggaccagc agcggccgca gccgcttgcc
     3061 gccccccatc gccgcatagc gcatggcttc gtagagccgg gcccgcgggt cgggaggcac
     3121 cgccagcagc tcggacagga aggcgtcgat ctcggcccgg acctggtcga gcgcgggcgg
     3181 gaggagcgag gcgtccgcca tggcggcggc tcagtccggg tcgaacgggg cggtgccggc
     3241 cggctggccg tcgcggccga actggatctt ctcgatcctc gcctgcgccg cctgaagccg
     3301 agcctcgcat tgctggcgca ggcggtcgcc ctccccgtag agctcgatcg actcgtcgag
     3361 cggggcttcc ccgctctcga gacggcggac gatctcctcc aggcgcttca gcgcggcttc
     3421 gaaggaaagc tctgaaatgg cttcgggcgg gtccggcatg agcggagcaa tgagccgcga
     3481 ggctagcgcg gtcaaggctc aggcggcgcg gtaccagtgg accccgtcga gcgcctcgcg
     3541 gacgacccgc ccctcgacct cgagccgccg caaatgggcc agcgcctcgc cggtggcgag
     3601 accgtacatg ccgtcgtcga tctgccgcgc gaacagggtg ccgaagcaat cgaccgcccg
     3661 cctcggctcg tcgagcctgg cgtggagcgc gtcgagccgc tcgcggtggc cgcgggcgag
     3721 cgcgtcgagg cgggcgtgga ggccggtgaa cggctcgccg tgcgagggga ggaccagcag
     3781 gctgtcgggc aggcccctca gcttcgcgat cgagtcgagc cattcgccca ggggatcggc
     3841 ctccggctcg ctgagcccga ccgagacgtt cgaggtgatc cgcggcagca cctggtcgcc
     3901 ggcgatcatc agcccgcccg cctcgtccac caggcaggca tgctcggggc aatggccgct
     3961 gccgatcagc acgcgccatt cgcgtcctcc ggcccgaagc gtgtcgccct cctgcattcg
     4021 cacgtagccg accgggatcg ggctcaccac cgaggcgaag cggccccagc ccttggccgc
     4081 ttccgcttcg atccgccctt cttcccagcc cgcggcgcgc caatgggcga tggcctcggg
     4141 gggcggggcg tcgcggacgt cgctggcgag catccggccg tacagccatt cgccgcgcgt
     4201 catccacagc gcgactcccc agcgctcggt cagccggccg gcaaggccga tatgatcggg
     4261 gtggaaatgg gtgcagatca cccgagtcac ccggcgaccc gccagcggcc cggcgaacag
     4321 ggcttcccag gcgtcgcggc agggcgcgat gtcgagcccg gtgtcgacca gggcgacgcc
     4381 ctcgccgtcg tcgagcaccc agacattgat gtgcttgagc gagccgggga tcggcagccg
     4441 cgcccagccg accccgtcgg ccagggcgat cagctcgccg gcgccgggac ccctgcggcc
     4501 gagcggatag gtcagtccct tgtggctgta cgcctcgtcc ggatcggtca gcgagacgtc
     4561 ggggctggcg ggctccaggc tgggctcagc tcttgaagag gtcatcggcc gtgggctcgc
     4621 gcggcggcgc ggcgcccgct tcctcggcgg catgcgcggc ttccgcttcg cgggccgccg
     4681 tatcgcgctc gcgccgcagc tcctcgatca gcgcctcgcg gtctccggcg agcctgtggt
     4741 cctgcggcgc cggctcgatc ccggccgcct tggccgcctt ggcgacgatc gcgtccaatt
     4801 cgcgctgcga gcagaggccg agcgtgacgg gatccttggg ctggatgttg gcgatgttcc
     4861 agtggctgcg gtcgcggatc gcagcgatgg tcgagcgggt ggtgccgatc agcttgccga
     4921 tctggccgtc ggagatctcc ggatggttgc ggatgatcca ggcgattccg tcgggcttgt
     4981 cctggcgctt ggagaccggc gtatagcgcg gacccttggt cctgcgggtc tggtccggtt
     5041 ccttctggat ccggaggcgg tagccggcgt ccgcctggcc ccggtcgatc tcctcctggt
     5101 tcagctcgcc ggcgcggatc gggtcgcggc cggtcagctt ggtcgcggcg gtgtcgtcgg
     5161 cgatcgcctg cacctccagg atgtgcaggc cgcagaattc agcgatctgc tcgaaggtga
     5221 gcgacgtgtt gtcgaccagc caggaggcgg tcgcatgggg catgagcggc tgggccacgg
     5281 tgagtctcca gaaacaagaa gggccgcccc ttcccggagc ggccgtcaca tatcgagcga
     5341 tctagtccag cctccgcgcg agggcaagaa gaatgccgcc attccgccgc attcacatct
     5401 tgattcggcc ccgaatcagg tcgagcttgg ccttggtaaa cggaggggaa cgttcatgaa
     5461 attcgtgacc ttgggggcgc tgatcgccgc ccatatcgct tgcgcggcgc aggccgccga
     5521 gctgccgcgc gagccgtcga ccgaggcgac ccgggtcggc accttcgccg gcgctcggat
     5581 gcgggtgccg ctgggcgcga cgaaggagaa gccgaatgcg ggcctcgcct tcaccgccac
     5641 ccagcgcacc ggtgacaccg gaacgctccg cttctccaag ggcatggagc tcggcttcgc
     5701 cggccgcgac aaggttcagc tctcgctcgc cggcaggcac gtgtcgcagc tgcggcaagg
     5761 cgccgaaggg ccgaacggcc gaaagcacgg cttctcgacg accggctggg tcattctggg
     5821 cgccagcgcg gtcatcatca tcggcggcgt gctgatattc gattaccttg gcgatcagag
     5881 cgaatgagag gcgaaatgaa gatgaagatg gcgatgatcg cggcgctcgt cgccgctcag
     5941 ctcccggccg ccggctatgc ggcgcaggcg accgagctcc cgcgcgagga atcggcggcg
     6001 cagcctcgcg ccggcgagga cgccgagggc gtgcccgcgg acgcgcaggc gcctgcgcct
     6061 gcggccgaga aaacgccggt gggcgcaccg gcgctggcgg cggaggagga tgagcccaag
     6121 aagggcaaga gcaccggcga caaggtcttg acgggcgccg ccgtggtcct cggagtcgcc
     6181 gcggtggctg tcgcggggct gctggtcgcg atcttcgtca actgaggcct aggccgccat
     6241 cggcagataa cccgattctc cggccacc
//
LOCUS       DASVQZ010000184         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_810613, whole genome shotgun sequence.
ACCESSION   DASVQZ010000184 DASVQZ010000000
VERSION     DASVQZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810613"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VF547_05100"
     CDS             <1..759
                     /locus_tag="VF547_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8262232.1"
                     /translation="GDQLEIDLRRALDQDEIEILFQPQVSVATRRIVGAEALARWRHP
                     RFGELGAITLFSVAERSDYLVQLSDHVQRKAVAAAAAWPEALGRLRLSVNITAADIVR
                     PGFAEQFIDLVAETGFDPARLTVEVTESGLIEDLGAAGNLLARLRQGGLRVAIDDFGT
                     GYSSLAYLKALPLDYLKIDKRLVEDISGSPRDQVVVRSVIDMARSLGLEVIAEGVETE
                     DQLALLAREGCTLYQGFLCSPPVEGARLAEMMGG"
     gene            complement(765..1001)
                     /locus_tag="VF547_05105"
     CDS             complement(765..1001)
                     /locus_tag="VF547_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262233.1"
                     /translation="MSRGNHEERFWMILISLMLAATTPPAVKPDSASPGKAVPARRAR
                     VVTRSKRLIELEAVVRSLGPSPAERVRGAGPEGL"
     gene            1129..>1575
                     /locus_tag="VF547_05110"
     CDS             1129..>1575
                     /locus_tag="VF547_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262234.1"
                     /translation="MDKPLRLFGLSASLIGTLGQLGVIAATAPAIGECIWSACRYGHP
                     GPIELFIGWEPYIDNPGVGVQPSRERRCPGEQAAGSRFRLDHAPLPAGFDAHAYPEGD
                     ILACVRLGGRGEVEAVRIIAGTGQPHLDSRLMRIVHRQWRFSPADGA"
BASE COUNT          217 a          547 c          556 g          255 t
ORIGIN      
        1 ggcgaccagc tcgagatcga ccttcgccgc gcgctcgacc aggacgagat cgagatattg
       61 ttccagccgc aggtgtcggt ggcgacccgg cggatcgtcg gcgccgaggc gctggcgcgc
      121 tggcgccacc cgcgcttcgg cgagctcggc gcgatcaccc tgttcagcgt cgccgagcgc
      181 tccgactatc tcgttcagct ctccgaccac gtccagcgca aggccgtcgc cgccgccgcc
      241 gcctggcccg aggcgctcgg ccggctgcgc ctttcggtca acatcaccgc cgccgacatc
      301 gtccggcccg gcttcgccga gcagttcatc gacctcgtcg ccgagaccgg cttcgatccg
      361 gcgcggctca ccgtcgaagt gaccgagagc ggcctgatcg aggatctcgg cgcggccggc
      421 aacctgctgg cgcggctccg ccagggcggg ctgcgggtcg cgatcgacga tttcggaacc
      481 ggctattcga gcctcgccta tctgaaggcg ctccccctcg actatctcaa gatcgacaag
      541 cgcctggtcg aggacatcag cggctcgccg cgcgaccagg tggtcgtgag gagcgtcatc
      601 gacatggccc gctcactggg cctggaggtg atcgcggaag gggtggagac ggaggaccag
      661 ctcgccctgc tcgcgcgcga gggctgcacc ctctaccagg gcttcctgtg ctcgccgccg
      721 gtggaggggg caaggctcgc ggagatgatg ggagggtagc cgccctacag cccttcgggg
      781 cctgcgccgc ggacgcgctc ggccggcgac ggacccagcg agcgaacgac ggcctcgagc
      841 tcgatcaggc gcttcgagcg ggttacgacg cgtgcgcgcc gggcgggaac ggctttccca
      901 gggctcgcgc tgtcaggctt cacggcgggc ggggtcgtcg cggcgagcat cagtgaaatc
      961 aggatcatcc agaagcgttc ctcgtggttg ccgcggctca acccgtcggc gctcggcggg
     1021 ttgcgggacg tccgaccagc cgcccgaaag gcgggctggc cgccgcgccg cgtatcatcg
     1081 ggttgatacg ggcttgttgt ttgcgatgtt ataatatatc ctgcatatat ggataagcct
     1141 ctccgactgt tcggtctctc cgcctcgctg atcgggactc tgggccagct cggggtgatc
     1201 gccgcgaccg cgccggcgat cggggagtgc atctggagcg cctgccggta cggccatccc
     1261 gggccgatcg agctgttcat cggctgggag ccctatatcg acaatccggg cgtcggcgtt
     1321 cagccgtcgc gcgagcggcg ctgtccgggc gaacaggcgg ccggctcgcg cttccgcctc
     1381 gaccatgcgc cgctgccggc cggcttcgac gcgcatgctt atcctgaagg ggacatactc
     1441 gcctgcgtga ggctgggcgg ccgcggcgag gtcgaggcgg tccggatcat cgcgggcacc
     1501 ggccagccgc acctcgactc ccggctgatg cggatcgtcc atcgccagtg gcgcttcagt
     1561 ccggccgacg gcgcc
//
LOCUS       DASVQZ010000185         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_521261, whole genome shotgun sequence.
ACCESSION   DASVQZ010000185 DASVQZ010000000
VERSION     DASVQZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521261"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /gene="ruvB"
                     /locus_tag="VF547_05115"
     CDS             complement(<1..921)
                     /gene="ruvB"
                     /locus_tag="VF547_05115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8262235.1"
                     /translation="MSDVDRLTTPSRRPEDLDAALRPKTLDEFVGQKAARENLRVFIQ
                     AARSRGDALDHVLFFGPPGLGKTTLAQIIARELGVGFRATSGPVIARSGDLAALLTNL
                     EEGDVLFIDEIHRLAPAVEEILYPAMEDRALDLMIGEGPSARSVRIDLPKFTLVGATT
                     RQGLITTPLRDRFGIPVRLNFYTVDELEEVVRRAAHLLGFSLTADGAREIARRSRGTP
                     RIAGRLLRRVRDFAHAAGHETVDAPDADAALNRLEVDQLGLDAMDRRYLTMIADLYKG
                     GPVGVETLAAGLSEPRDTIEEVIEPFLIQLG"
     gene            complement(914..1246)
                     /locus_tag="VF547_05120"
     CDS             complement(914..1246)
                     /locus_tag="VF547_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262236.1"
                     /translation="MSAAPYWTPYRKAAATAIVTWGGFIPIFFWLIMLVGGSVSTGII
                     PLVVFGLPVIIAGFLWDTIGACPACGKSPFALRDRIGPAAISTGLWWPERQCSKCGYD
                     LTEGTDDV"
     gene            complement(1243..1806)
                     /gene="ruvA"
                     /locus_tag="VF547_05125"
     CDS             complement(1243..1806)
                     /gene="ruvA"
                     /locus_tag="VF547_05125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8262237.1"
                     /translation="MSADHAVIDVNGVGYLVFASSRTLSSLGGTGAEVVLHTVMQVSE
                     DDIRLIGFASGEERDWFTLLRSVQGVGAKVALAILSALAIDELHRAVASGDSAMIARA
                     TGVGPKLAQRIAHELKDKAGGAALGGAIGAPPAPGSVTADAASALQNLGFRPAEASAA
                     VAKAEAELGPGATLDALVRLALKKAAR"
BASE COUNT          290 a          624 c          633 g          259 t
ORIGIN      
        1 gccgagctgg atcagaaacg gctcgatcac ttcctcgatc gtgtcgcgcg gctcggacag
       61 gccggcggcg agcgtctcca cgccgaccgg cccgcctttg tagagatcgg cgatcatcgt
      121 caggtagcgc cggtccatcg cgtccaaacc gagctggtcg acctcgagcc ggttgagcgc
      181 ggcgtccgca tcgggggcgt caacggtctc gtggcccgcc gcatgggcga aatcgcgcac
      241 ccgccgcagc agccggccgg cgatacgcgg cgtgccccgc gagcggcggg caatctcgcg
      301 ggcgccgtca gccgtgagcg agaagccgag caggtgcgcg gcccggcgca ccacctcctc
      361 gagctcgtcc accgtataga aattcagccg gacggggatg ccgaaccggt cccgtagcgg
      421 cgtcgtgatc agcccctggc gcgtggtcgc gccgacgagc gtgaattttg gcaggtcgat
      481 cctgaccgac cgggccgagg gcccctcgcc gatcatcagg tccagcgccc ggtcctccat
      541 cgcgggataa aggatctcct cgacggcggg cgccaggcgg tggatttcgt cgatgaagag
      601 gacgtcgcct tcctcgaggt tggtgagcaa agccgccaga tctcccgacc tggcgatcac
      661 cggccccgac gtcgcccgga acccgacgcc gagttcgcgc gcgatgatct gcgccagcgt
      721 cgtcttcccc agccccggcg ggccgaagaa cagcacatgg tcgagcgcat ccccccggct
      781 ccgcgccgcc tggatgaaca cgcgaagatt ctcccgcgcc gccttctgtc cgacgaactc
      841 gtccagcgtc ttcggccgaa gcgcggcgtc gaggtcctcc gggcggcggc tgggcgtggt
      901 gaggcggtcg acgtcagaca tcgtcggtac cctcggtcag atcatagccg cacttcgagc
      961 attgccgctc cggccaccag aggccagtgc tgatcgccgc cgggccgata cggtcgcgaa
     1021 gcgcaaaggg cgatttcccg cacgccgggc aagcgccgat cgtgtcccag aggaacccgg
     1081 caataatgac cggcaacccg aaaacgacca gcggaattat accggtggag acgctcccgc
     1141 cgaccagcat gatgagccag aagaagatcg ggatgaatcc accccaggtg acgatcgccg
     1201 tcgccgccgc tttgcgatac ggggtccagt aaggcgcagc gctcatcgcg ccgctttctt
     1261 gagcgccagc cggacgaggg cgtcgagggt ggcgcccggg ccgagctcgg cttcggcttt
     1321 ggcgaccgcg gcggaggctt cggcgggcct gaaaccgaga ttctggaggg ccgaggcggc
     1381 gtccgcggtg acgctaccgg gggcgggagg agcgccgatc gcgccgccca gcgccgcgcc
     1441 gccggccttg tccttgagtt cgtgcgcgat gcgctgggcg agcttggggc cgacgccggt
     1501 ggcgcgcgcg atcatcgcgc tgtcgcccga cgccacggcg cggtgcagct cgtcgatcgc
     1561 cagtgccgag aggatggcga gcgcgacctt ggcgccgacg ccctggaccg agcggagcaa
     1621 ggtgaaccag tcccgctcct cgccgctggc gaagccgatc aggcggatat cgtcttcgct
     1681 gacctgcatc accgtgtgca gaacgacttc ggcgcccgtc ccgccgaggc tggagagtgt
     1741 gcggctggag gcgaagacga gatagccgac gccgttgacg tcgatgaccg cgtggtcggc
     1801 cgacac
//
LOCUS       DASVQZ010000186        12546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_426821, whole genome shotgun sequence.
ACCESSION   DASVQZ010000186 DASVQZ010000000
VERSION     DASVQZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12546
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426821"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VF547_05130"
     CDS             complement(<1..1075)
                     /locus_tag="VF547_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262238.1"
                     /translation="MTLLVAEQRFAAPDQFQAAGAGFARGLKVAPLASGGFVAAWLHD
                     GRLVGQMFDRNGAPAGGTFALTSNPASFPDRIELTPLAGGGFVAAWEVFAGSQSRVEY
                     RRFDAAGQPLGPEQPVSPGYGQAPSVAPLADGGFVIAFTAVDGESSVGIHAQRFDSQG
                     AKTGGEIPVNTTTAGQQYHSRAATLPGGAFLITWLDPAALRGQIFDSAGQKVGGELDI
                     APAGNYAASLAALPSGNVVVTYGAGEIMAKILTPAGAVAVPEFQVNTGTAGTQSMASI
                     AVLGTGEFLITWRDYVAPAGYDQDADIKHGEVRGQLFAADGARIGGEYLVNNVTARGQ
                     TEPVSAAFGSGDLATLWIDYSGGD"
     gene            1276..2277
                     /locus_tag="VF547_05135"
     CDS             1276..2277
                     /locus_tag="VF547_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX8262239.1"
                     /translation="MAGVLSGIRIVELAGIGPAPFAGMMLADHGATVIRVEREDRPPV
                     IPPEFDVLGRSRASTIRLDLKSEKGAARVRELARDCDGLIEGYRPGVMERLGLGPETL
                     LEDNPRLVYGRMTGWGQDGPLAPHAGHDIDYIALAGALHTYGRAGGPPTPPVNAVGDF
                     GGGGMLLAFGMLAGILSARTTGKGTVIDCAMVDGAALLSALTWSLKAAGMWKDERGVN
                     LLDTGRAYYDVYRCADGEWVAVGALEPEFFAVLKERMGLECGQHDPALRDELEAAFLR
                     HPRQHWTNILQQSDACFAPILSLSEAPSHPHNRMRGTFLNISGVVQPAPAPRFKDPD"
     gene            2277..3419
                     /locus_tag="VF547_05140"
     CDS             2277..3419
                     /locus_tag="VF547_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8262240.1"
                     /translation="MLDTSRRTAFAPEHEQFRDSVRRFFERELIPHLSRWEEEGIIDR
                     GFWTKAGEAGLLCPTVPEEYGGPGLDFGFNAVIDEELAYAGSTAGITLHSDIVADYLV
                     AYGSEEQKAHWLPRMVSGETATAIAMTEPGAGSDLQGIRTSARRDGNHYVLNGSKTYI
                     TNGQHADLVIVVAKTDPDAGAKGVSLILVEADREGFARGRNLDKIGFHSQDTSELFFN
                     DVRLPITNCLGEEGSGFAYLMNQLPQERLSIACAAQAAAQRAFDEAVRFTKDRTAFGK
                     TVFDFQNTKFTLADLAAKLQVGWAHLDWAIARHVAGELTAAEASAAKLWHTELQWEAC
                     DASLQLHGGAGYMNEYLIARLWRDARVTRIFGGTNEIMKEVVSRSI"
     gene            4038..4772
                     /locus_tag="VF547_05145"
     CDS             4038..4772
                     /locus_tag="VF547_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262241.1"
                     /translation="MTPWLDGMDDPGPFRRMRLDPGRYHPRIARPLAPAGGQALWLPD
                     QAGERRHVTGAQNQLESLIDDLRTICRVVQPTPATLAVHGHEIRNLLILAATEVEMHW
                     RGVLAANGRTVEARTAQYVRLAGPLRLRDFTVRFPPCPEIEPVPPFAGWNAEESTKSL
                     SWYAAYNGVKHNRELEFERASLGHAFDAVAACAVMLVAQFGDEALTPEHSSFLSVEAP
                     RWPVEEMYVAPRDGGTWQPTPYPSLG"
     gene            complement(5176..6102)
                     /locus_tag="VF547_05150"
     CDS             complement(5176..6102)
                     /locus_tag="VF547_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8262242.1"
                     /translation="MASPDLNLLHTLDVLLSEGSVARAARRLRLSPSAMSRALARLRS
                     VTGDPLLVRAGRGLVPTPRADEMRDRVRLLVEDARAVLRPAGQVDLLTLKRTFTLRAS
                     DGIAETFGPALIGRVRKEAPGVQLRFVRKLDKDSQGLRDGTTDLETGVVDRAIGPEIR
                     SRALFTDRYVGVVRAGHPLARAAVTSADYAGWPHVIAWRPGLDLAGIDERLSELGMAR
                     TVASSVDGFSAALALARGSDLIATVPGRHTAGLRQGLHSFALPLSLPDFTVSLLWHPR
                     LDGDPAHRWLRALVHDACRPGAPETWSSEGSG"
     gene            6230..6775
                     /locus_tag="VF547_05155"
     CDS             6230..6775
                     /locus_tag="VF547_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262243.1"
                     /translation="MPSNALDRFIAGIRGDWGPISTQVVARCRQHLAELTLADPAEDW
                     LTALREERPEARELLRDPTHGYMLLAHSERRGLYRAPHDHGRAWVVYGVQSGELEVGT
                     YAKVADGAGQVRLVRRDMHVLRPGEARAYLPGDIHDTRCLSDTALLLRFTERDLRHED
                     RIERKLTRFVERHGEWTEPAE"
     gene            6772..8124
                     /locus_tag="VF547_05160"
     CDS             6772..8124
                     /locus_tag="VF547_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8262244.1"
                     /translation="MTALSASQRIATLAVLAAMALAVLDMGMVNVALPILAQRFEAAP
                     SDAILVISACQAALLVGLLPCAHIADRTSPRAMFRGGVALFCGASLLCALAPSLTFLI
                     AARVLQGLGAAAVMALGVALLRSALGSDRLGSAIAWNALTVALSSAAGPALGAVILSF
                     ASWPWLFLAGLPLGAAALLAAGALPDRPATRDAVDAPAIALHAAAVILFLAAAKAAAS
                     DAMLALILACSAAPLLALLMRRTRGRHSPMLPADLLGRPSFAVQASASACCFIGQSLG
                     LVALPFHLQRAVPNDLVAAGLVVTCWPLGVAATSLAMGRMSGLDRGVQCAAGGGLLAA
                     GLLLSASLPLQWGAMPLAAGAALCGIGFGLFQLANNRALFLAAPAERAAAAGGIQGTA
                     RLTGQTTGTLIMALIYSWSSMALAPRVGLVIGAAFASAAAMLAARRAQAGTGEDSRGP
                     "
     gene            complement(8147..9313)
                     /locus_tag="VF547_05165"
     CDS             complement(8147..9313)
                     /locus_tag="VF547_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8262245.1"
                     /translation="MLATMLAAAAAASVPFAVPSVTDGRAIHGQIDIPASPPKGVVVL
                     VPGTGDHSRDLRLNSNPKEAPVLVFQDLARRLNRAGLAVARFDKRGFSCVKQGKTGSD
                     GWGCHDAEETPTVTWATSAGDVEAVVRHAEAQPGGDCLVLVGHSEGTVHIARLIEGER
                     VRPAGFIGLGPVMDSFASLLHDQAVERLTDGLLAMDKDRDGVTSRTEFLSQLPGSRLA
                     ADFDSNGECTAFCLAELEKRSPVGPAGITALEVRALRDRLQALYRRKVEAQAGPAAPP
                     LYAFPDGTVYSTTDWRRQVLTEWVPSALRLSRYRGPVVLYWGTADRQVNLAQGLAAVR
                     RFGPAQAVNRVYEGLGHMLGTHAVAGPIREDVAQSIVADAERMAASCAKERAAR"
     gene            complement(9381..9713)
                     /locus_tag="VF547_05170"
     CDS             complement(9381..9713)
                     /locus_tag="VF547_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8262246.1"
                     /translation="MAVTGSCHCGKVRIEAPSPPEWVASCNCSLCTKTAWLVAYYPDG
                     AVKVEGETVPYVWGDRMIGIHHCPVCGCGTHWRTLGEDFGKMGVNARLLDGFDPGAVE
                     VRLFDNRD"
     gene            9853..10485
                     /locus_tag="VF547_05175"
     CDS             9853..10485
                     /locus_tag="VF547_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8262247.1"
                     /translation="MRVEAKRLWWSASLFVLLAAGGLIGLDRTIALWTAGGARGSIWD
                     QGTRVLDLLTLKDLSNFLLGALLLASAAALIALRRTRRIGWPVLYLALVQALATVAAD
                     LSKPPFGRLRPFEAEAAGNIDSWFVGANSFPSGHVAFYAGLFFPLAVLLPRWAALWML
                     PPLFVAVARVIENDHYLSDVAASLALASALAAALSPLARRGRAPTGEIAG"
     gene            10482..11222
                     /locus_tag="VF547_05180"
     CDS             10482..11222
                     /locus_tag="VF547_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8262248.1"
                     /translation="MKIVGSYVSPYVRKVLACLALKGLDYEIDPITPFYGNDEFERLS
                     PLRRIPVLVEGDLALCDSSVICAWLDEAYSQRPLLPAGANDRARARWLEEYADTRLGD
                     VFIWGLFYPKFVHPIVWGEPGDPARIEKTLAVDLPRELDWLEAQLPADGFLFGDEIGL
                     ADIAVATFFRNGAHVGVEVDAARWPRTADFIGRALAQPCFADLLPFEQVQLGASPAGR
                     RQALLAAGAPLAAETMATREPRRGVMRL"
     gene            11303..11473
                     /locus_tag="VF547_05185"
     CDS             11303..11473
                     /locus_tag="VF547_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262249.1"
                     /translation="MSLAGLAVSTVYQYVLNSPPEDMKGGAMLAMNLAIWAVAIGLLA
                     YALGMRKRGTLR"
     gene            complement(11470..12024)
                     /locus_tag="VF547_05190"
     CDS             complement(11470..12024)
                     /locus_tag="VF547_05190"
                     /inference="COORDINATES: protein motif:HMM:NF004861.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8262250.1"
                     /translation="MLARVGITGASGCGVTTLGGALAESLGAVHIDTDDHFWIATDPP
                     YRLKRDVPDRLAGIAAEQARSGRWVMSGTLDGWAEPALRDAQLIVFLEVPTEIRLDRL
                     RRRERARFGDSLLPGGAMHETHREFIEWAARYEAGTEPGRSRPRHEQWLAGLALPVLR
                     LDGTRPTTELVAAVLDPARGGGSG"
     gene            complement(12038..12526)
                     /locus_tag="VF547_05195"
     CDS             complement(12038..12526)
                     /locus_tag="VF547_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8262251.1"
                     /translation="MIRHWVHAAAILTLLSLAPAAAASDPAAAKGDLPASAAAAAATV
                     DAFHAALRRGDTRAAAALLADDALIFEEGRAERSRAEYEAAHLGADAAFSKAVPGAVT
                     RRRGAAAGDFAWIATEGLSKGKYRGAAVNRLTDETMVLRRIGQAWKIVHIHWSSAQSA
                     VE"
BASE COUNT         1774 a         4481 c         4377 g         1914 t
ORIGIN      
        1 gatcgccgcc gctgtagtcg atccacaagg tggccaggtc gccggagccg aaggccgccg
       61 agaccggctc ggtctggccg cgggcggtca cgttgttgac cagatattcc ccgccgatcc
      121 tggcgccgtc cgccgcgaac agctggcccc gcacctcgcc gtgcttgatg tcggcgtcct
      181 ggtcatatcc ggccggagcg acatagtcgc gccaggtgat caggaactcg ccggtcccga
      241 gcaccgcgat cgacgccatg gactgcgtgc ccgccgtgcc ggtgttgacc tggaactcgg
      301 gcacggcgac ggcgccggcc ggagtgagga tcttcgccat gatctcgccg gcgccatagg
      361 tgacgacgac attgcccgac ggaagcgcgg cgaggctggc cgcgtaattg ccggccggcg
      421 cgatatcgag ctccccgccg accttctgcc cggcggagtc gaagatctgg ccgcgcaggg
      481 cggcgggatc gagccaggtg atcaggaagg cgccgccggg cagggtcgcc gcccgcgaat
      541 gatattgttg gcccgcggtc gtggtgttga ccgggatctc gccgccggtc ttggccccct
      601 gggagtcgaa ccgctgcgcg tggatgccga cgctgctttc gccgtcgacg gcggtgaagg
      661 cgatgacgaa gccgccgtcg gcgagcggcg cgacgctggg cgcctgcccg tagcctgggc
      721 tgaccggctg ctcggggccg agcggctggc cggccgcgtc gaagcggcga tattcgaccc
      781 gggattggga gccggcgaag acctcccagg cggcgacgaa gccgccgccg gcgagcggcg
      841 tcagctcgat tcggtcgggg aagctcgccg gattgctggt cagagcgaag gtgccgccgg
      901 ccggcgcgcc gttccggtcg aacatctgcc cgaccaggcg gccgtcgtgc agccaggcgg
      961 cgacgaagcc gcccgaggcc aaaggcgcga ccttgaggcc ccgcgcgaag ccggccccgg
     1021 cggcctggaa ctggtctggt gcggcgaagc gctgttccgc gacaagaagc gtcatgagcg
     1081 aggtccccgc cctgatcgga aggcttccag cctatccgat ccgacgcccc ggctcaaccc
     1141 gcgcccgaac cccgaatcgc ccgcatcgca ccccgctgga tcgccccgtc gcattcgcca
     1201 aacgcgcctt cccgaccctg ccccctgcgg cgtgcgcgat tgccgagcga ggccccggca
     1261 ccctatgcag ggcccatggc aggggtgctt tccggaatcc gcatcgtcga gctggccggg
     1321 atcggccccg cgccgttcgc cgggatgatg ctcgccgacc acggcgccac cgtcatccgg
     1381 gtcgagcggg aggaccggcc gcccgtgatc ccgcccgagt tcgacgtgct cggccgctcg
     1441 cgcgcctcga cgatccggct cgacctcaag tccgagaagg gcgccgcccg cgtccgcgag
     1501 ctggctcggg actgcgacgg gctgatcgag gggtacaggc ccggcgtgat ggagcggctc
     1561 gggctcgggc cggagacgct gctcgaggac aatccgaggc tggtctacgg gcggatgacc
     1621 ggctggggcc aggacgggcc gctggcgccg catgccggcc acgacatcga ctacatcgcg
     1681 ctcgccgggg cgctccacac ctacggccgc gccggcgggc cgccgactcc gccggtcaac
     1741 gcggtcggcg atttcggcgg cggcgggatg ctgctcgcct tcggcatgct cgccgggatc
     1801 ctgtcggccc ggacgaccgg caagggcacg gtgatcgact gcgcgatggt cgacggcgcc
     1861 gccctgctct cggccctgac ctggtcgctg aaggcggcgg ggatgtggaa ggacgagcgc
     1921 ggggtcaacc tgctcgacac cggccgcgcc tattacgacg tctaccgctg cgccgacggc
     1981 gagtgggtgg cggtcggtgc gctcgagccg gaattcttcg cggttctgaa ggagcggatg
     2041 gggctcgagt gcggtcagca cgatcccgcg ctccgcgacg agctcgaggc ggcgttcctc
     2101 cgccatcccc gccaacattg gaccaacatc ctccaacaaa gcgacgcctg tttcgcgccg
     2161 atcctctcgc tttccgaggc gccttcgcat ccccacaaca ggatgcgcgg cactttcctc
     2221 aacatatcgg gcgtcgtcca gcccgccccc gccccccgct tcaaggatcc cgactgatgc
     2281 tcgacacgtc ccgccggacc gccttcgccc ccgagcacga gcagttccgc gactcggtcc
     2341 gacgcttctt cgagcgcgag ctcatccccc acctgagccg ttgggaggag gagggaatca
     2401 tcgaccgcgg cttctggacc aaggcgggcg aggcggggct tctgtgcccg accgtgcccg
     2461 aggaatatgg cggccccggc ctcgacttcg gcttcaacgc cgtcatcgac gaggagctcg
     2521 cctatgccgg ctcgaccgcg ggcatcaccc tccattcgga catcgtcgcc gactatctcg
     2581 tcgcctacgg ctccgaggag cagaaggccc actggcttcc ccgaatggtg tcgggcgaga
     2641 ccgccaccgc catcgcgatg accgagcccg gcgccggctc cgacctccag ggcatccgca
     2701 ccagcgcgag gcgcgacggc aaccattatg tcctcaacgg ctcgaagacc tacatcacca
     2761 acggccagca tgccgacctc gtcatcgtcg tcgccaagac cgaccccgac gccggcgcga
     2821 aaggcgtctc gctgatcctg gtcgaggccg accgcgaggg cttcgccagg ggccgcaacc
     2881 tcgacaagat cggcttccat tcgcaggaca cgtccgagct cttcttcaac gacgtccgcc
     2941 tgccgatcac caactgcctc ggcgaggagg gctcgggctt cgcctacctg atgaaccagc
     3001 tcccccagga gcgcctctcc atcgcctgcg ccgcccaggc cgcggcccag cgcgcgttcg
     3061 acgaggcggt caggttcacc aaggatagga cggcgttcgg caagaccgtg ttcgacttcc
     3121 agaacaccaa attcaccctc gccgacctcg cggcgaagct ccaggtcggc tgggcccatc
     3181 tcgactgggc gatcgcccgc catgtcgccg gcgagctcac cgccgccgag gcctcggccg
     3241 ccaagctctg gcacaccgag ctgcaatggg aggcctgcga cgcctcgctc cagctgcacg
     3301 gcggcgccgg ctacatgaac gaatatctga tcgcccgcct ctggcgcgac gcccgggtga
     3361 cccggatctt cggagggacg aacgagatca tgaaggaggt ggtgagccgg tcgatctgag
     3421 cggggagcgc ccgcaaacgc ccctcccggc ccgctccgcc gccccggaca ggccgctttc
     3481 cacccctcgc cgagcgactg gaaacaaagc ctttttccgt caaagttcat gaaattcatc
     3541 gagtcgggcg ctcttttcgt gaacttcgcg gggagaatca tggccggcca agggcttggc
     3601 cccgccgcgc tccgaagctc gacccgaacc tcgccggaca tcggcgccac cctcctttcc
     3661 catgcgcctt ccccagcctt cttgaacgtg ccaagagctg gaacatagca gaaacatgac
     3721 gtcctggaaa gtcggcaggg cgcaggcctg gtcaggggaa atggcggcag cctatatttc
     3781 cgggacccgg tgcggtacgg gcagcgcttg ggaaacatgc tgtcgctgat tggtgggggc
     3841 ttgaccgggt gacgacggcg cagggacgtg aagctccggg ctggtacgtc cgggacccgg
     3901 ggagcggcga gggccatcac ctctggtaca tcgccgagga ccgggcggta cgcgcggacg
     3961 tcacgaaccc cagcggcggc gccggcacct gtttcacggc ggagcccggg gagacgattt
     4021 ggcaagccct gcggcgaatg accccgtggc tcgacggcat ggacgatccc ggtcccttcc
     4081 gccggatgag gctcgacccc gggcgctacc atccccggat cgcgcggccg cttgccccgg
     4141 ccgggggtca ggctctctgg cttcccgacc aggccgggga gcggcgccat gtcaccggcg
     4201 ctcagaacca gctcgaatcg ctgatcgacg atctgcggac catctgccgg gtcgtccagc
     4261 cgactccggc gacgctagcg gtccatgggc acgagatccg caacctgctg atcctcgccg
     4321 cgaccgaggt cgagatgcac tggcgaggcg tgctggccgc caacggcagg acggtcgagg
     4381 ctcgcaccgc ccaatatgtc cggctcgccg gcccgctccg gctccgggat ttcacggtcc
     4441 gcttccctcc ttgccccgaa atcgagcccg tccctccgtt cgccggctgg aacgccgagg
     4501 aatccaccaa gtcgctcagc tggtacgcgg cctataacgg cgtgaagcat aaccgcgagc
     4561 tcgagttcga acgggcctcg ctcggccatg ccttcgacgc ggtggcggcc tgcgccgtga
     4621 tgctggtcgc gcagttcggg gacgaggcgc tgactcccga gcattcgagc ttcctgtcgg
     4681 tcgaagcgcc gcgctggccg gtcgaagaga tgtatgtcgc cccgcgcgac ggcgggactt
     4741 ggcagccgac cccgtacccg agcctgggct gattgcatgg cataaggaga tcatgaagga
     4801 ggtggcgtcg aggtcgatcc gaggccccgg ccgggcttcg gatgggggcc gcagcccgcc
     4861 tcgcccgggc cggttcgccg cgcgcttccg ccgccgccta gcgctcgggc agtcggattg
     4921 cactgtttcc gttcgggctg agctgtcgaa gccctctcct tttctcttat ggatcgacaa
     4981 ggaaagccat tcgacaggct cagggcgaac gggcgggggt gatccgaccc aatccctcga
     5041 tgctccacag cgctcggcag ccggatcgcg cgctcttgcg ttcgggctga gcttgtcgaa
     5101 gccgtctcct tttctctcat ggaacgacaa ggaaagccct tcgacaggct cagggcgaac
     5161 gggtgagaga gtgactcagc ctgatccctc gctgctccag gtctcgggcg cgcccgggcg
     5221 gcaggcgtcg tggacgaggg cgcgcagcca tcgatgggcg gggtcgccgt cgagccgcgg
     5281 gtgccagagc agcgaaaccg tgaagtcggg caggctgagc gggagcgcga aactgtgcag
     5341 gccttgccgg aggccggccg tgtgacggcc cggaaccgtg gcgatcagat ccgagccgcg
     5401 ggcgagggcc agggccgccg agaagccgtc gacgctgctc gcgaccgtcc gcgccatccc
     5461 cagctcggac aggcgctcgt cgatgcccgc caggtcgagg ccggggcgcc aggcgatgac
     5521 gtgaggccag ccggcatagt ccgccgacgt tacagccgcc cgagcgagcg ggtgaccggc
     5581 tcgaaccacg ccgacgtagc ggtccgtgaa gagggcgcgg ctgcggatct cggggccgat
     5641 cgcgcggtcg acgacccccg tctcgaggtc cgtcgtgccg tcgcgcagcc cctggctgtc
     5701 cttgtcgagc ttgcgcacga agcgcagctg cacgccgggc gcctccttgc gcacccggcc
     5761 gatcagcgcc ggcccgaacg tctcggcaat cccgtcgctc gcccgaaggg tgaaggtccg
     5821 cttcaaggtc agcaggtcga cctggcccgc cggccgaagc acggcgcgcg cgtcctccac
     5881 caacagcctg acccggtcgc gcatttcgtc ggcgcggggc gtcggcacga gcccgcggcc
     5941 ggcgcgcacc agcaacggat cgccggtcac ggagcgcagg cgggccaggg cccggctcat
     6001 cgccgaaggg ctgagccgaa gcctgcgcgc ggcgcgcgcg acgctccctt cggaaagcag
     6061 gacgtcgagc gtgtgaagca ggttcaagtc gggcgaggcc ataggccgcc ttagcgcccc
     6121 tgcgtgccga tatatagcgt caaacgcaac gataggttgt gatcggtgcg tcttccgcca
     6181 cggacgatga cggtcctatc ctcctgtccg ccccagggag gagatcgaca tgccttcgaa
     6241 cgcgctagac aggttcatcg ccggcatccg cggcgactgg gggccgatca gcacgcaagt
     6301 cgtcgcgcga tgccgccagc atttggcgga gctcaccctg gccgatccgg ccgaggactg
     6361 gctgaccgcg ctacgcgagg agcggccgga agcgcgcgag ctcctgcgcg atcccaccca
     6421 cggctacatg ctgctggcgc acagcgagcg gcgcggcctc tatcgcgctc cgcacgacca
     6481 tggccgggcc tgggtggtct atggggtgca gtccggagag cttgaggtcg gcacctatgc
     6541 caaggtggcc gatggggccg gccaggtccg gctcgttcgg cgcgacatgc atgtgcttcg
     6601 gccgggcgag gcgcgcgcct acctgcccgg cgacatccac gacacgcgct gcctcagcga
     6661 cacggcgctg ctgttgcgct tcaccgaacg cgacctgcgc cacgaagatc ggatcgagcg
     6721 caagctgacc cgcttcgtcg agcgccacgg cgagtggacc gagccggccg aatgaccgcg
     6781 ctttccgctt cgcagcggat cgccacgctg gcggtcctgg cagccatggc gcttgcggtc
     6841 ctggacatgg ggatggtcaa cgtcgccctc ccgatccttg cgcagcgctt cgaggctgcg
     6901 ccgtcggacg cgatcctcgt catcagcgcc tgccaggccg ccttgctcgt cggcctcctg
     6961 ccctgcgcgc acatcgccga caggaccagc cctcgcgcca tgttccgcgg aggcgttgcg
     7021 ctgttctgcg gggcgagcct gctgtgcgcg ctcgccccgt cgctgacgtt cctgatcgcg
     7081 gcgcgcgtgc tgcagggcct cggcgcggct gcggtcatgg cgctcggcgt ggccctgctg
     7141 cgctccgctc tcggcagcga ccggctcggc tccgcgatcg cctggaacgc gctcaccgtc
     7201 gcgctcagct cggcagcagg gccggcgctc ggggccgtga tcctctcctt cgcttcgtgg
     7261 ccctggctgt tcctggccgg cttgccgctc ggcgcggcgg ccttgctcgc ggccggcgcg
     7321 ctgccggaca ggccggccac ccgggacgcc gtcgatgctc cggccatcgc cctccacgcg
     7381 gcggcggtga tcctgttcct tgccgcggcc aaggcggcag cgtccgatgc gatgctcgcg
     7441 ctgatcctgg cctgctcggc ggcgccgctc ctggcgctgc tgatgcgccg gacccgcggc
     7501 cgccattcgc cgatgctccc ggccgacctg ctgggacggc cgtccttcgc cgtgcaggca
     7561 agcgcatcgg cctgctgctt catcggccag tcgctggggc tcgtcgcgct tccgtttcac
     7621 ctccaaaggg ccgtgccgaa cgacctggtg gcggccgggc tggtcgtcac ctgctggccg
     7681 ctcggcgttg ccgccaccag tctcgccatg ggccggatga gcggactgga tcgcggggtg
     7741 caatgtgccg caggcggcgg ccttctggcg gccggcctgc tgctctcggc aagcttgccg
     7801 ctccagtggg gcgcgatgcc gctggcggcc ggcgcagcgc tctgcggaat cggcttcggg
     7861 cttttccagc tggccaacaa ccgtgccctg ttcctcgccg caccggcgga acgcgcggct
     7921 gcggccggag gcatccaggg aacggcgcgg ctgacagggc agaccacggg cacgctgatc
     7981 atggccctga tctactcgtg gagctcgatg gcgcttgcgc cgcgggtggg gctcgtcatc
     8041 ggggccgcct tcgcaagcgc ggccgcgatg ctcgccgcgc ggcgagcgca ggccgggacg
     8101 ggagaggatt cacgcggtcc ttgaggcaga tgggcaatcc gcccgctcag cgcgccgcgc
     8161 gttccttcgc gcaggacgcc gccatgcgct cggcgtcggc gacgatgctc tgcgcgacgt
     8221 cctccctgat cgggccggcg accgcgtgag tcccgagcat gtggccgagg ccctcgtaga
     8281 cccggttgac ggcctgcgcg ggaccgaaac gccggacggc ggcgaggccc tgggcgaggt
     8341 tgacctggcg gtcggccgtt ccccagtaga ggacgaccgg accccggtag cggctgagcc
     8401 gaagcgcgct cgggacccac tcggtgagaa cctggcggcg ccagtccgtg gtcgaataga
     8461 ccgttccgtc gggaaaggcg tagagaggcg gcgcagcggg tcccgcctgg gcctcgacct
     8521 tgcggcgata gagcgcctgc agccggtccc gcagcgcacg gacctcgagc gcggtgatcc
     8581 cggccggtcc caccggcgac cgcttttcca gctcggcgag acagaaggcg gtgcactccc
     8641 cgttcgaatc gaaatccgcc gccagccggc tgccgggaag ctgggagagg aactccgtgc
     8701 ggctcgtgac tccgtcccgg tccttgtcca tcgccagaag gccgtccgtc agccgctcga
     8761 ccgcctggtc gtggagcagg gacgcgaagc tgtccatcac cggcccgagg ccgatgaagc
     8821 cggccggccg cacccgttcc ccttcgatga ggcgggcgat gtggaccgtg ccctcgctgt
     8881 gtcccacgag gacgaggcaa tcgccgcccg gctgggcctc ggcgtgccgg acgaccgctt
     8941 ctacgtcccc ggcgctggtc gcccaggtca cggtgggcgt ctcttcggca tcgtggcagc
     9001 cccagccgtc gctgccggtc ttgccttgct tgacgcagct gaacccccgc ttgtcgaacc
     9061 gcgcgacggc gaggccggca cggttcagcc gccgtgcgag atcctggaag accagaacgg
     9121 gggcctcttt cggattgctg ttcaggcgaa ggtcgcggct gtgatcgccc gttcccggca
     9181 cgagcacgac gacgcccttc ggcggcgaag cggggatgtc gatctggccg tggatcgccc
     9241 tgccgtcggt gacggagggc accgcgaacg ggactgaggc cgcggcggcg gcggcaagca
     9301 tcgtggcgag catgctgccc ctctctgaag ctcacgacct agaagcatgg ccgtgccggc
     9361 gaagcaatgt gcgattccgt ctagtcgcga ttgtcgaaca gcctcacctc gaccgccccc
     9421 ggatcgaagc cgtcgagaag ccgcgcgttc acgcccatct tcccgaaatc ctcgcccagc
     9481 gtccgccagt gggtgccgca gccgcagacc gggcaatggt ggatcccgat catccggtcg
     9541 ccccagacgt aggggacggt ctcgccctcc accttgaccg cgccgtcggg ataataggcg
     9601 accagccagg cggtcttggt gcacagcgag cagttgcacg aagcgaccca ttcgggcggc
     9661 gacggcgcct cgatccggac cttcccgcaa tgacagcttc cagtgaccgc caaggcactc
     9721 tcccttccgt tcgaacctgc gagcccgtcc aggccgtggc tagaccgcac ggcgcgcgat
     9781 ttccactccc ggaattgacg gagcgggcgc ggcctgctgt attagccgac taagtcagct
     9841 cgggagggac ggatgcgggt cgaagccaag aggctgtggt ggtcggcgtc gctgttcgtc
     9901 ctgctggccg cgggaggcct gatcggtctt gaccggacga tcgcgctctg gacggcgggc
     9961 ggagcccgcg gatcgatttg ggaccagggc acgcgcgtcc tcgatctgct cacgctcaag
    10021 gacctctcca acttcctgct cggcgccctg ctgctggctt cggcggcggc gctgatcgcg
    10081 ttgcggcgca cccgccggat cggctggccc gtgctgtacc tcgccctggt tcaggccctg
    10141 gcgacggtgg cggcggatct ctccaagccg ccgttcgggc gcctgcggcc gttcgaggcc
    10201 gaagcggcgg gcaacatcga cagctggttc gtgggcgcca attcctttcc gtcggggcat
    10261 gtcgccttct atgccgggct gttcttcccg ctcgccgtcc tgctcccgcg ctgggccgcg
    10321 ctgtggatgc tgccgccgct cttcgtcgcg gtggcgcggg tgatcgagaa cgaccattat
    10381 ctcagcgatg tcgccgcttc gctcgctctg gcctcggcgc tggcggcggc gctgtcgccg
    10441 ctggcccgcc gcgggcgcgc gccgaccggg gagatcgccg gatgaagatc gtgggaagct
    10501 atgtcagtcc ctatgtgagg aaagtgctcg cctgcctggc gctgaagggg ctcgactatg
    10561 agatcgaccc gatcacgccc ttctacggca atgacgagtt cgagcggctg agcccgctga
    10621 ggcgcatccc ggtgctggtc gaaggcgacc tcgccttgtg cgattcctcg gtgatctgcg
    10681 cctggctcga cgaggcttat tcgcagcggc cgctcctgcc cgcgggcgcg aacgaccgcg
    10741 cgcgggcgcg gtggctcgag gaatatgccg acacgcgcct cggcgacgtc ttcatctggg
    10801 ggctgttcta cccgaagttc gtccatccga tcgtctgggg cgagcccggc gacccggcgc
    10861 ggatcgagaa gaccctggcc gtcgacctgc cgcgagagct cgactggctg gaggcccagc
    10921 tgccggcgga cggcttcctg ttcggagacg agatcggcct cgccgacatc gccgtcgcca
    10981 ccttcttccg caacggcgcc catgtcgggg tcgaggtgga cgcagcgcgc tggccgcgga
    11041 cggcggattt catcgggcga gcgctcgccc agccctgctt cgccgacctg ctgccgttcg
    11101 agcaggtcca gctcggcgca agccccgcgg gccggcgcca ggccctcctc gccgccggag
    11161 cgccgctcgc ggccgagacg atggcgaccc gcgagccgcg ccgaggggtg atgcggctct
    11221 gagccgccgg cttgccaagt gcgggctgct cggcgcgctg ctgctgctgg cgcgaagccg
    11281 gcacgcggtc gccgccttcg ccgtgtcgct ggcgggcctc gccgtcagca ccgtctacca
    11341 atatgtcctg aactcgccgc ccgaggacat gaagggcggc gcgatgctgg cgatgaacct
    11401 ggcgatctgg gcggtcgcga tcggattgct ggcctatgcg ctcgggatgc gcaagcgcgg
    11461 gacgctgcgc tagccggatc ctccgcctcg agcaggatcg aggacggcgg cgaccagctc
    11521 ggtcgtcggg cgagtgccgt cgagcctcag caccggcagg gcgagtccgg ccagccattg
    11581 ctcgtgcctc gggcggcttc gtcccggctc ggtcccggcc tcgtagcggg ccgcccattc
    11641 gatgaactcg cgatgagtct cgtgcatcgc tccgccggga agcagcgagt cgccgaaacg
    11701 cgcccgctcg cgccggcgga ggcggtcgag gcggatctcg gtcggcactt cgaggaagac
    11761 gatcagctga gcgtcgcgca gcgccggttc cgcccagccg tcgagcgtgc cggacatgac
    11821 ccatcgcccg ctccgggcct gctccgcggc gatgcccgcc agccggtcgg ggacgtcgcg
    11881 cttcaaccgg tagggcggat cggtggcgat ccagaaatgg tcgtccgtgt cgatatggac
    11941 ggcgccgaga ctctccgcca gcgcgccgcc cagggtcgtg acgccgcagc ccgaggctcc
    12001 ggtgatcccg actcgcgcga gcatcggggc cccgccccta ctccaccgcc gactgcgcgg
    12061 acgaccagtg gatatggacg atcttccacg cctggccgat ccggcgcagg accatggtct
    12121 cgtcggtcag ccgattgacg gcggcgccgc gatacttgcc cttgctgagg ccctcggtgg
    12181 cgatccaggc gaagtcgccc gccgcggcgc cgcggcgccg ggtcacggcc cccgggaccg
    12241 ccttcgagaa ggcggcgtcg gcgccgagat gcgcggcttc atattccgcc ctgctgcgct
    12301 ccgcccggcc ctcctcgaag atcagggcgt cgtcggcgag caaggcggcc gcggcccggg
    12361 tgtcgcctcg gcgcaaggcg gcgtggaagg cgtcgaccgt cgccgccgcc gcggcggcgg
    12421 acgccggcag atcgcccttc gcggccgcgg gatcgctcgc ggcggcggcc ggagcgaggg
    12481 acaggagcgt gaggatcgcc gccgcgtgaa cccagtgccg aatcattttt ccgatgcctt
    12541 ccagtt
//
LOCUS       DASVQZ010000187         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_521696, whole genome shotgun sequence.
ACCESSION   DASVQZ010000187 DASVQZ010000000
VERSION     DASVQZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521696"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VF547_05200"
     CDS             <1..1290
                     /locus_tag="VF547_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX8262252.1"
                     /translation="RNERAIARYLRRLEGEEVFNRFEAERYLLLAGDLPGHAVRLALR
                     SAGQARGEVVGEVTVVRTPGLVDFSVQNFGGRELGRWGALLRGQLYGLTGLGDRTTLA
                     LFSTPDFDEQHTVQLGHDFRIGGEGLALSGQLTYAWASSDLGDPAIDIDARTLLATVE
                     ASYPFVRRLSHSVRGAVGFDLIDQDVDFGGLRLNRDRLRIAFARLSAEAQGVGDARRY
                     TLAEPRWRASGSLQLRHGIGVLGASSGCGPAFVNCSGGAVPPTRLEGDPTAFVARADA
                     LGEFRPIPNVTFALGGIAQHSSRPLFAFEEFSAGNYTVGRGYDPGTLLGDSGIGLQAE
                     LRVGRAFPRTPEAWAFQFYGFYDRAWVWNEDRIAAAPTRDRLSSAGGGVRAAWGNRAR
                     LDLVVAVPLERAGLQTERPDPRLLVSLTTRLWPWSLQ"
     gene            1287..>2658
                     /locus_tag="VF547_05205"
     CDS             1287..>2658
                     /locus_tag="VF547_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262253.1"
                     /translation="MTANPSRLRARMLLGCAFAALTLAGFGGRADAQGFNASPAVVAG
                     SVTFDRTPGLDTITINSPSAIIDWRLTFSPPAPDPIVFLPAGNTGIFQNAPGGGDFAV
                     LNRILPVGASRIRMDGTVIGRLQTAAGNVPGGTVVFYSPTGLIIGSSAVFDVGRLLLT
                     TLNPAVDASGNFFTAGTFQLNGGQFPPASIVTEAGARFNAPADGSWVAMVAPRIDHGG
                     AVRVNGSAAYVAAEAVSIRIDQGLFDIDIQVGTSDSNPIVHRGTTSGPASSGAGDNHV
                     IYLAAAPQNNPMTLLLAGSAGFDAAVSASVENGAIVLSAGHDVQGRAISEVPEGIPSG
                     DFLIRSGTYTSDVFGRASQDFIVGDAVPGGVVFEQDLSIAARRLAMLTADEGYSLRVG
                     GSATVSAANLRPVPVDLVTIDVAGGEAAIFAANGGTIAIGGNATVDASARGATDGTGV
                     AGRGSGG"
BASE COUNT          347 a         1028 c          892 g          391 t
ORIGIN      
        1 cgcaacgagc gcgccatcgc ccgctatctg cgccgtctgg aaggcgagga ggtgttcaac
       61 cgcttcgaag ccgagcgtta cctgctcctc gccggcgacc tgcccggcca cgccgtccgc
      121 ctcgcgctgc gctctgccgg ccaggcgcgc ggcgaggtcg tcggcgaagt gacggtggtc
      181 cgcacgcccg gcctcgtcga tttcagcgtc cagaatttcg gcgggcgcga gctcggccgc
      241 tggggcgccc tgctgcgcgg ccagctctac ggcctgaccg gcctcggcga ccgaaccact
      301 ttggccctct tctcgacgcc cgatttcgac gagcagcaca ccgtccagct cggccacgac
      361 ttccgcatcg gcggcgaagg cctcgcgctc tccggccagc tcacctacgc ctgggccagc
      421 tcggacctcg gcgatcccgc catcgacatc gatgcccgga ccctgctcgc caccgtcgag
      481 gcgagctacc ccttcgtccg gcgcctctcg cacagcgtcc gcggcgcggt cggcttcgac
      541 ctcatcgacc aggacgtgga tttcggcgga ctccgcctca accgcgaccg cctccggatc
      601 gccttcgccc gcctctccgc ggaggcgcag ggcgtcggcg atgcgcggcg ctacacgctg
      661 gcagagccgc gctggcgggc gagcggatcg ctccagctgc gccacggcat cggtgtgctc
      721 ggggccagca gcggctgcgg accggccttc gtcaattgca gcgggggcgc cgtgccgccc
      781 acccggctgg aaggcgatcc caccgccttc gtcgcccgcg ccgacgcgct cggcgagttc
      841 cggccgatcc cgaacgtcac cttcgcgctc ggcggcatcg cgcagcattc gagccggccg
      901 ctcttcgcct tcgaggaatt ctcggccggc aactacacgg tcgggcgcgg ctacgatccg
      961 ggcacgctgc tcggcgacag cggcatcgga ctgcaggcgg agctgcgcgt cggccgcgcc
     1021 tttccgcgca cgccggaagc ctgggcgttc cagttctacg gtttctacga ccgcgcctgg
     1081 gtctggaacg aggaccgcat cgccgcggcg ccgacgcggg accggctgag ctcggccggc
     1141 ggcggcgtcc gcgccgcctg gggcaaccgc gcccggctcg acctcgtcgt cgccgtcccg
     1201 ctggagcgcg cggggctcca gaccgagcgg cccgacccgc gcctcctcgt ctcgctcacc
     1261 acccgcctct ggccatggag cctccaatga cggcgaaccc ttcgcgcctc cgcgcccgca
     1321 tgcttctcgg ctgtgcgttc gccgctttga cgctggcggg tttcggcgga cgagccgatg
     1381 cgcagggctt caacgcctcg ccggccgtcg tcgccggcag cgtcacgttc gaccgcacac
     1441 cgggcctcga cacgatcacg atcaactcgc cgtcggcgat catcgactgg cggctgacct
     1501 tttcgccccc ggcccccgat ccgatcgtct tcctccccgc cggcaacacc ggcatcttcc
     1561 agaacgcgcc gggcggcggc gacttcgcgg tcctgaaccg gatcctgccg gtgggcgcga
     1621 gccgcatccg gatggacggg acggtgatcg gccgcctgca gaccgcagcc ggcaacgtgc
     1681 cgggcggcac cgtcgtcttc tacagcccca ccggcctgat catcggctcc agcgccgtct
     1741 tcgacgtcgg ccggctgctg ctgacgacgc tcaacccggc ggtggatgcg agcggcaact
     1801 tcttcacggc cggcaccttc cagctgaacg gcggccagtt tccgccggcg tcgatcgtga
     1861 ccgaagccgg cgcccgcttc aacgcgccgg ccgatggaag ctgggtcgcc atggtcgcgc
     1921 cgcgcatcga tcatggcggg gccgtccggg tcaacggctc ggccgcctac gtcgccgccg
     1981 aagccgtctc gatccgaatc gaccagggtc tgttcgacat cgacatccag gtcggcacca
     2041 gcgattccaa cccgatcgtt caccgcggca caacaagcgg gccggcgagc agcggcgctg
     2101 gcgacaatca cgtcatctat ctcgccgcgg cgccgcagaa caacccgatg accctgctgc
     2161 tcgcgggcag cgccggcttc gatgcggcgg taagcgcgag cgtggagaac ggcgccatcg
     2221 tgctttccgc cgggcacgac gtgcagggcc gcgccatttc cgaagtgccg gagggcatcc
     2281 cgtccggcga cttcctgatc cggtccggaa cctacacgtc ggacgtcttc ggccgcgcct
     2341 cgcaggactt catcgtcggc gacgcggtgc cgggcggcgt ggtcttcgag caggacctgt
     2401 cgatcgcggc gcggcgcctc gcgatgctca ccgcggacga aggctattcg ctgcgcgtcg
     2461 gcggcagcgc caccgtctcc gccgcgaacc tgcggccggt gcccgtcgac ctcgtcacga
     2521 tcgacgtcgc cggcggagaa gccgcgatct tcgcggcgaa cggcggcacc atcgcgatcg
     2581 gcggcaacgc gaccgtcgac gcctcggcgc gcggcgcgac cgacggcacg ggcgtggctg
     2641 gcaggggcag cggcgggc
//
LOCUS       DASVQZ010000188         6060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_41099, whole genome shotgun sequence.
ACCESSION   DASVQZ010000188 DASVQZ010000000
VERSION     DASVQZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41099"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VF547_05210"
     CDS             complement(<1..674)
                     /locus_tag="VF547_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8262254.1"
                     /translation="MVTTVFFFLLVLVVLFLMSGIKIVRQGYHYTIEYFGRFTAVAHP
                     GFNFYMPFFYRVGRKVNMMEQVLDIPGQEIITKDNAMVSTDGVVFFQVLDAAKAAYEV
                     SDLYVAIMQLTTTNLRTVMGSMDLDETLSKRDEINARLLSVVDQATESWGVKITRVEI
                     KDIRPPADIVNAMGRQMKAEREKRAVILEAEGSRASAILRAEGEKQGQILEAEGRREA
                     AFRDAEA"
     gene            746..1543
                     /locus_tag="VF547_05215"
     CDS             746..1543
                     /locus_tag="VF547_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX8262255.1"
                     /translation="MHDLRLCRSLLFLPASNPRAIEKARGLPADMVVLDLEDAVKPED
                     KDAARAAAVAAAAEGFGGLCAIRINSLDPWHGPDLEAVAASRADFAIVPMTPGPEAPS
                     AAGRATGKPILAMIETARGVLAAAAIAPETAGLIAGTNDLAADLGLRPGGDRSALATA
                     LQTILLAARSAGVAAFDGVYNRLDDAAGFEAQCEEGRRLGFDGKSLIHPSQVEPANRA
                     FGPTEEEVAAAKRLLAAATGGAERFEGEMIERMHVEQARRVLARARA"
     gene            1761..3248
                     /locus_tag="VF547_05220"
     CDS             1761..3248
                     /locus_tag="VF547_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8262256.1"
                     /translation="MAVRFLAALLILLCATAAQAISPEALRRHIDVLAADGFEGREPG
                     TVGEQKTIAYIARQMQSFGLEPAGPRNGWYQALEVVVRRPGEQSSVWQGRGKRAEKIE
                     LDRDGLVLIGRTARETLDEAPVVFAGHGAVMPDKGIDQLAGADLKGAVVLILYDSPPA
                     AGFPAYSQRVAAVAGRGAAAVIGIVGEELPWPVVQRTYDAGQKRLAVAAPAPIQGAMS
                     QAAAERLVRLAGGDLARLLDSPGPSFAPVALRLRATLDVATAIDTIATRNVLGRLRGV
                     GGTGESLLYLAHWDHLGLCAPEERDRICNGAVDNASGIAALLEIARALAGGPRPRRDV
                     LFLATTSEELGLLGAEYFATRPVVPLRSIVAAINMDTVAIAPRGEKVAVMGRGRPRLD
                     ALIDRTIAESGRILDPDDEAAGFVERQDGWALARAGVPAVMVGGSFSDMKKLGAFIAG
                     PYHSPADNPGPALVLDGAAEDAELMVALGRKLADPALYDHAAERR"
     gene            3426..3947
                     /locus_tag="VF547_05225"
     CDS             3426..3947
                     /locus_tag="VF547_05225"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX8262257.1"
                     /translation="MSRAYSAALPIVYVVLRLLVLVNWLYGLAILALLLVMPNERWIM
                     SAFELTPSPEADRLIMGLRVVALLGLVTIPLNYVVLKRLLGMVGTVRMGDPFVAANAE
                     RLQAIAWALLGLQLLSLVIGGIGKALSTPAHPVDLDAGFSINGWLAVLLTFVLARVFA
                     QGALMREDLEGTV"
     gene            3949..4206
                     /locus_tag="VF547_05230"
     CDS             3949..4206
                     /locus_tag="VF547_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8262258.1"
                     /translation="MAILVKLDDLLHARRMTLTELAERVGITIVNLSILKTGKARAIR
                     FSTLEAICDALGCQPGDLLEFERGDMEDGGEQAVASGGGRA"
     gene            4203..4919
                     /locus_tag="VF547_05235"
     CDS             4203..4919
                     /locus_tag="VF547_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262259.1"
                     /translation="MNAGSFVHGGLMAVHIGAGLIALAAGAVAMAMRKGGTGHGRAGT
                     GFVAAMLVLGLTAAILSPLKAEPESPLGGILVCYFVLTAWMAARRRDGRMGLFEQAAC
                     LFALAGGAVTLWAGLAGESTTPAGRGPVLIFGAVCLLAGLLDLNALLRRRLAPAQRLA
                     RHVWRMCVAFFIATGSFFLGQQDVMPKAVQGSAWLFVPAFAPFAAMLFWLVRLRFARA
                     IARLKLAAGPVAARLPQPQA"
     gene            4924..5412
                     /locus_tag="VF547_05240"
     CDS             4924..5412
                     /locus_tag="VF547_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262260.1"
                     /translation="MLERLFPRQVDKGFTGLRPALWLLGALIAVRLVMSVNSIVNTET
                     VAARADGIPLGSYGPEAAREVLLLFALMALGHLALTLVALAVLIRWRALVPFVYLVLL
                     GEAVARRAIAQGHAAPGARMTDIAWFVNYGLLALLVLGLALSLLPARRERRRSPDLED
                     SR"
     gene            5409..5855
                     /locus_tag="VF547_05245"
     CDS             5409..5855
                     /locus_tag="VF547_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262261.1"
                     /translation="MTEVSPIRLWVMRAMYLFMAVGIGNMVWPLIISHASTVPRMTGV
                     AWALIGTIGLLSLIGLRYPLQMIPLLLVELSWKAIWLAFFALPRWLDGTLDEAMRTSV
                     FETSFGALLLVVIPWRHVWANYVVRPGDPWTFRRTANSGPAAPAAS"
BASE COUNT          795 a         2223 c         2078 g          964 t
ORIGIN      
        1 gcctcggcgt cgcggaaggc ggcctcgcgg cggccttcgg cctcgaggat ctggccctgc
       61 ttctcgccct cggccctgag gatcgccgaa gcccggctgc cctcggcctc gaggatgacg
      121 gcgcgcttct cgcgctcggc cttcatctgc cggcccatgg cgttgacgat gtcggccggc
      181 gggcggatgt ccttgatctc gacccgggtg atcttcacgc cccagctctc ggtcgcctgg
      241 tcgaccacgc tgagcaggcg ggcgttgatc tcgtcgcgct tggatagggt ctcgtcgagg
      301 tccatcgagc ccatcacggt cctcaaattg gtcgtggtga gctgcatgat cgcgacgtag
      361 aggtcggaga cctcataggc ggccttggcg gcgtcgagca cctggaagaa gacgacgccg
      421 tcggtcgaga ccatggcatt gtccttggtg atgatctcct ggccggggat gtcgagcacc
      481 tgctccatca tgttcacctt gcgccccacc cggtagaaga acggcatgta gaaattgaag
      541 cccgggtgcg cgaccgcggt gaagcgtccg aaatattcta tcgtgtaatg atagccttgc
      601 cggacgatct tgattcccga catgaggaac aggacgacca ggacaagcag gaagaaaaag
      661 acggtggtga ccatatacgt gcgcctcccc ttgcggaccg cggcataatg cgggcaagcg
      721 gggcgggctc caagcgaaat cggacatgca cgacctgcgc ctctgccgtt ccctgctctt
      781 ccttcccgcg tccaatccgc gcgcgatcga gaaggcgagg ggcctgcccg ccgacatggt
      841 cgtcctcgac ctcgaggacg cggtgaagcc cgaggacaag gacgccgccc gggccgccgc
      901 ggtggcggcc gcggccgaag gcttcggggg cctgtgcgcg atccggatca attcgctcga
      961 tccctggcac gggccggacc tcgaggcggt ggcggcaagc cgcgccgact tcgcgatcgt
     1021 cccaatgacc ccgggccccg aggcgccgag cgcggccggc cgagcgaccg gcaagccgat
     1081 cctggcgatg atcgagaccg cccgcggagt cctcgccgcc gccgccatcg ccccggagac
     1141 cgccgggctg atcgccggaa ccaacgacct cgccgccgac ctcggccttc gccccggcgg
     1201 cgaccgctcg gcgctcgcca ccgccctcca gaccatcctg ctcgccgccc ggtcggccgg
     1261 ggtcgccgcc ttcgacgggg tctacaaccg gctcgacgac gccgccggct tcgaggccca
     1321 gtgcgaggaa ggccggcggc tcggcttcga cggcaagtcg ctgatccacc cgagccaggt
     1381 cgagcccgcg aaccgtgcgt tcggcccgac cgaggaggag gtggccgcgg cgaagcgcct
     1441 gctcgccgcc gccaccggcg gggccgagcg gttcgagggc gagatgatcg agcgcatgca
     1501 cgtcgagcag gcaaggcggg tgctggccag ggctcgcgcc taggccgatc gacattcacc
     1561 gggtggcacc cttgtcccct ccccgacggg gagagggtcg ggagaggggg ctcggcgtct
     1621 gtgatcgctc ccggccgctg agccccctca ccccagccct ctccccactg gggagaggga
     1681 caaggggcca cccctaagac ggcggcagtc cgaagggcct ccctcttgcc acccgcgcct
     1741 tcggcgactt aagcgagggg atggccgttc gctttctcgc cgcgcttctg atcctcctct
     1801 gcgccaccgc cgcccaggcg atctcgcccg aggcgcttcg ccgccacatc gacgtgctcg
     1861 ccgccgacgg gttcgaggga cgcgagccgg gcacggttgg cgagcagaag acgatcgcct
     1921 atatcgcgcg gcagatgcag tcgttcgggc tcgagccggc cggcccgcgc aacggctggt
     1981 accaggccct ggaagtggtg gtccgccgcc ccggcgagca gagcagcgtc tggcagggga
     2041 gggggaagcg ggccgagaag atcgagctcg accgcgacgg cctcgtcctg atcgggcgga
     2101 ccgcgcgcga gactctggac gaggcgccgg tcgtcttcgc cggccacggc gcggtgatgc
     2161 cggacaaggg catcgaccag ctcgccggcg ccgacctcaa gggcgccgtc gtcctcatcc
     2221 tctacgactc gccgccggcg gccggctttc ccgcttattc gcagcgggtc gccgcggtcg
     2281 ccgggcgcgg cgcggcggcg gtgatcggga tcgtcggcga agagctgccc tggccggtcg
     2341 tccagcgcac ctacgatgcc ggccagaagc ggctcgccgt cgccgctccc gctccgatcc
     2401 agggcgcgat gtctcaggcc gccgccgaac ggctggtgcg gctcgccggc ggcgacctcg
     2461 cccggctgct cgactcgccg ggcccgtcct tcgcgccggt cgccctcagg ctcagggcga
     2521 cgctcgacgt cgccaccgcg atcgacacca tcgccacccg caacgtcctc ggccggctga
     2581 ggggcgtcgg cggcaccggc gagagcctcc tctacctcgc ccactgggac catctcggcc
     2641 tgtgcgcgcc cgaggagcgc gaccggatct gcaacggggc ggtcgacaac gcgagcggca
     2701 tcgccgccct gctggagatc gcccgcgcgc tcgccggcgg gccgcggccg cggcgcgacg
     2761 tgctgttcct cgccaccacc tcggaggagc tcgggctgct gggcgccgaa tatttcgcga
     2821 cccgcccggt cgtgccgctg cgctcgatcg tcgccgcgat caacatggac acggtcgcga
     2881 tcgcccccag gggcgagaag gttgcggtga tgggccgcgg ccgcccccgc ctcgacgcgc
     2941 tgatcgaccg cacgatcgcc gaatccgggc ggatcctcga tccggacgac gaggccgccg
     3001 gcttcgtcga gcgccaggac ggctgggccc tggcccgcgc cggagtgccc gcggtcatgg
     3061 tcggcggctc cttctccgac atgaagaagc tcggcgcctt catcgccggg ccctatcact
     3121 cgccggccga caatcccggg ccggccctcg tcctcgacgg cgccgccgag gatgcggagc
     3181 tgatggtcgc gctcgggcgc aagctcgccg atccggcgct ctacgaccac gccgccgaac
     3241 gccgctagag cgctcgcagt cggatcgcgc cctcgcccgc ggccggccgt cgccgcgatc
     3301 agccggggcc ggcgcatgcc cccattcgag ccgaagtcgc cgctgaattg acgagcaacg
     3361 atatgggact tgacgactgt cgataaggta attatcgtat atcgatatca accgaggacg
     3421 ccgtcatgtc tcgtgcctat tccgccgccc tgccgatcgt ctacgtcgtg ctgcgcctgc
     3481 tggtcctggt caactggctc tacggcctcg ccatcctcgc ccttctcctc gtcatgccca
     3541 acgagcgctg gatcatgagc gcgttcgagc tgacgccctc gcccgaggcc gaccggctga
     3601 tcatgggcct tcgggtcgtc gccctcctcg gcctcgtcac catcccgctc aactatgtcg
     3661 tgctcaagcg gctgctggga atggtcggca cggtgcggat gggcgatccg ttcgtcgccg
     3721 ccaacgccga gcgcctgcag gcgatcgcct gggcgctgct cgggctgcag ctgctcagcc
     3781 tcgtcatcgg cggaatcggc aaggcgctct cgacgcccgc gcatcccgtc gacctcgacg
     3841 ccggcttctc gatcaacggc tggctcgccg tgctcctgac cttcgtcctg gcccgcgtgt
     3901 tcgcgcaggg cgcgctgatg cgcgaagacc tggaagggac ggtctgaaat ggcgatcctc
     3961 gtcaagctgg acgacctgct tcacgcccgg cggatgactc tgaccgagct cgccgagcgg
     4021 gtcggcatca cgatcgtcaa cctgtcgatc ctgaagaccg gcaaggcgcg cgcaatccgc
     4081 ttctcgacgc tggaggcgat ctgcgacgcg ctcggctgcc agccggggga cctgctggag
     4141 ttcgagcgcg gggacatgga ggacggcggg gaacaggccg tcgcgagcgg gggagggcgg
     4201 gcatgaacgc gggaagcttt gtccacggcg gcctgatggc ggtccatatc ggcgcgggcc
     4261 tgatcgccct cgcggcgggc gccgtcgcga tggcgatgcg caagggcggg accgggcacg
     4321 gacgcgccgg aaccggcttc gtcgccgcga tgctcgtcct cggcctcacc gcagccatcc
     4381 tgtcgccgct caaggcggag ccggaatcgc cgctcggcgg catcctcgtc tgctatttcg
     4441 tcctcaccgc ctggatggcc gctcggcgcc gcgacggcag gatgggcctg ttcgagcaag
     4501 ccgcctgcct gttcgccctg gccggcggcg cggtcaccct gtgggcgggc ctcgccgggg
     4561 agtcgacgac cccggcgggc cgcggccccg tcctcatctt cggcgccgtc tgcctgctcg
     4621 ccggcctgct cgacctcaac gcgctgctcc gacgcaggct cgcccccgcc cagcgcctcg
     4681 cccgccacgt ctggcggatg tgcgtcgcct tcttcatcgc caccgggtcc ttcttcctcg
     4741 gccagcagga cgtgatgccg aaggcggtgc aaggctcggc ctggctgttc gtccccgcct
     4801 tcgcgccgtt cgcggcgatg ctcttctggc tggtgcgcct gcgcttcgcc agggcgatcg
     4861 cccgcctgaa gctcgccgcc ggccccgtcg ccgcccgcct cccccagccg caggcctgac
     4921 gccatgctcg agcgcctctt tccccgccag gtcgacaagg gcttcaccgg gctccgcccc
     4981 gccttgtggc tgctcggcgc gctgatcgcg gtgaggctcg tgatgagcgt caactcgatc
     5041 gtcaacaccg agacggtcgc cgctcgcgcc gacggcatcc cgctgggcag ctacgggccg
     5101 gaggcggcgc gcgaggtgct gctgctgttc gcgctgatgg cgctcggcca ccttgccctc
     5161 accctggtcg cgctcgccgt cctgatccgc tggcgggcgc tcgtcccgtt cgtctacctg
     5221 gtcctgctcg gcgaggcggt cgcccgccgc gccatcgcgc aaggccatgc cgcgcccggg
     5281 gccaggatga ccgacatcgc ctggttcgtg aactacgggc tgctcgcgct gctcgtcctc
     5341 ggactggcgc tttcgctcct tcccgcccgc cgcgagcgcc gacgctcgcc cgacctggag
     5401 gattcacgat gaccgaggta tcgccgattc gtctgtgggt gatgcgcgcc atgtatctgt
     5461 tcatggccgt cgggatcggc aacatggtct ggccgctgat catcagccat gcctccacgg
     5521 tgccgcgcat gaccggagtc gcctgggccc tgatagggac catcggcctg ctgtccctga
     5581 tcgggcttcg ctatccgctg cagatgatcc cgctgcttct ggtcgagctg agctggaagg
     5641 cgatctggct cgccttcttc gccctgccgc gctggctcga cggaacgctc gacgaggcga
     5701 tgcggacgtc ggtcttcgag acctcgttcg gggccctcct gctggtcgtc atcccctggc
     5761 gccatgtctg ggcgaactac gtcgtccggc cgggcgatcc gtggaccttc cgccggaccg
     5821 cgaattcggg gcccgcggct cccgccgcct cctgagcatg ggcgcggctt gggcggcggt
     5881 cggcgaatga aacgtcttgt ggcggcggcc gcggcggtcc ttatcgtggc ggccgtggcc
     5941 ctgacgctcg accctctcgg cagcttcaac gcgctgatgc ccaaggatcg ggcatcggcg
     6001 cgcgtggcgg aggggatcgc cttcgggccc gggccgcgga tgaggctcga cgtctacgcc
//
LOCUS       DASVQZ010000189         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_330547, whole genome shotgun sequence.
ACCESSION   DASVQZ010000189 DASVQZ010000000
VERSION     DASVQZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330547"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VF547_05250"
     CDS             complement(<1..383)
                     /locus_tag="VF547_05250"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8262262.1"
                     /translation="MHLPPEFQLVAACCRWPPSPERDEAVRAAAASVDWPLVLRIAER
                     HRVEGLVWNALLRSQAPVPAEAAGHLQAAAARIARQNLVLTGESLRLSGLLEQAGVRH
                     LFVKGVTLGVLAYGSIGPKMGWDIDL"
     gene            532..972
                     /locus_tag="VF547_05255"
     CDS             532..972
                     /locus_tag="VF547_05255"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX8262263.1"
                     /translation="MAAPFARPRRARLGPSDWLMLAEALAALALASLAIALLPFRKVA
                     DAASAPVRKAVPADAQTVRRVRSAVRAWARRVPWRTVCFQKGLALHWMLRRRGIGSVL
                     LYGARRDGEGLAAHVWVDVDGETVIGGDEAPNFTCLARFPPDSR"
     gene            1107..>2619
                     /locus_tag="VF547_05260"
     CDS             1107..>2619
                     /locus_tag="VF547_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8262264.1"
                     /translation="MGALSLVTGAAALAQVPSTSSPTTSTPPPPTPTPPHIPERAPAP
                     LPSHVAPTPGSVQQPPSRSDYSPPSSLVQTSPSGVQPAPLAGSRSPTLPGPTRDPLNI
                     DPGKDPILQLAWSQAAPEAFRSVVATAVERHPATQEARATSAEAEAVVGEARERRLPS
                     IDLSVSSYKVLARDFSEDPQNIIERSRPSQRTDAILNINQTVFDFGASADRVLAAGAR
                     LRAAAAEAEASADRIALNTVASWYDVFAYRALVALTESFVANQRELREAVEERVRQGV
                     SAQGDTARVESYLASAATRLAGFRRQLANAEARYTELIGSPPPAYLERAPIPQTPTLT
                     RDAAALAALSTPAARSAQAMADSARREARATKSDRLPQITAGIDAGRYGVFENDRDYD
                     IRGRVTLRQRLFGGTEARLAQARARARSADARASRVRDEASRDAAIAWSDVQALEEQL
                     RALEASYLASRQSRDVLIERFINSRGNLFDVVAAEDAYFETATAFIRTLSELDA"
BASE COUNT          371 a          967 c          911 g          370 t
ORIGIN      
        1 aggtcgatgt cccagcccat cttcggcccg atcgagccat aggcgagcac ccccagggtg
       61 actcccttga cgaagaggtg gcgaaccccg gcctgctcca gaagcccgct caggcgaagc
      121 gattccccgg tgaggacgag attctgccgg gcgatccggg ccgcggccgc ctggagatgc
      181 ccggccgcct cggccgggac cggggcttgc gaccgtaaga gcgcgttcca gacgagtccc
      241 tcgacccggt ggcgctcggc gattcgaagg accaaaggcc aatccaccga tgccgcggcg
      301 gcccggacgg cctcgtcgcg ctccggcgag ggcggccagc ggcagcaggc ggcgacgagc
      361 tggaattcgg ggggcaggtg cacgccggga gctgctttct ctgggaagcg cacgtcctag
      421 agcctgatcg tttccggtgg aagcgcctcg cgcttctccg gcggcgcgag cgagggcgct
      481 ggatccgaca ggagttgcca ccttcgaggg ccatcctgta acgccctggc gatggccgcc
      541 cccttcgcca gaccccgacg cgcccggctt gggccttccg actggctgat gctggcggag
      601 gcgctcgccg ccctcgcctt ggcctcgctc gccattgccc tgctgccgtt ccggaaggtc
      661 gccgacgccg cctcggcccc ggtccggaag gcggttccgg cggacgccca gaccgtccgg
      721 cgggtccgct cggcggtgag ggcctgggcc cggcgggtgc cttggaggac ggtctgcttc
      781 cagaagggac tggcgctcca ctggatgctt cgccgccggg gcatcggttc ggtgctcctc
      841 tacggcgcgc ggcgcgacgg cgaagggctc gcggcccatg tctgggtcga cgtcgacggg
      901 gagaccgtca tcggcggcga cgaggctccg aacttcacct gcctggcgcg cttcccgccc
      961 gattcccgct gaccgcggag cgccggcagc gggggtcaat cccttgaggg tgcaggtttt
     1021 cctagtctcc cgagcctaac gtgggtaagg gaccgacggc agagggttct tgacagacga
     1081 tgctggggtt gaagaaggga gcgctgatgg gcgcgctgtc gctcgtcacg ggcgcggcgg
     1141 cgctcgccca ggttccctcg accagctctc cgacgacgag cactccgccg ccgcccaccc
     1201 cgaccccgcc gcacatcccc gaacgcgcgc cggcgcctct gccctcgcac gtcgccccca
     1261 ctccgggttc ggtccagcag ccgccgtcgc ggtccgatta ttcgccgcct tcctcgctgg
     1321 tccagacgag cccgtcgggg gttcagccgg cgccgctcgc cggttcgcga agcccgaccc
     1381 tgccgggccc cacccgggat ccgctcaaca tcgatccggg caaggatccg atcctgcagc
     1441 tggcctggag ccaggccgcg ccggaggcgt tccggagcgt cgtcgcgacc gcggtcgagc
     1501 gccatcccgc cacccaggag gcgagggcga ccagcgccga ggccgaggcc gtcgtcgggg
     1561 aagcccggga acggcgcctg ccctcgatcg atctcagcgt ctcctcctac aaggtcctcg
     1621 cccgggattt ctccgaggat ccgcagaaca tcatcgagcg ctccaggccg tcccagcgca
     1681 ccgacgcgat cctcaacatc aaccagaccg tcttcgattt cggggcgagc gccgacaggg
     1741 tgctcgcggc cggagcgagg ctcagggccg ccgccgccga ggccgaggcg agcgccgacc
     1801 ggatcgctct caacacggtc gcatcctggt acgacgtctt cgcctaccgg gcgctggtgg
     1861 cgctgaccga atccttcgtc gccaaccagc gcgagcttcg cgaggcggtc gaggagaggg
     1921 ttcggcaggg agtctcggcg cagggcgaca cggcccgggt ggaaagctat ctcgcctcgg
     1981 ccgcgacccg gctcgccggc ttccgaaggc agctcgccaa tgccgaggcg cgctataccg
     2041 agctgatcgg ttcgccgccg cccgcttatc tggaacgcgc cccgattccc cagaccccga
     2101 ccctcacccg agacgcggca gcgctcgcgg ctctcagcac cccggccgcc cgctccgccc
     2161 aggcgatggc cgattcggcc cggcgcgagg cgcgggcgac caaatcggac cggcttcccc
     2221 agatcacggc cggaatcgac gccggacgct acggtgtctt cgagaacgac cgcgattacg
     2281 acatccgcgg ccgggtcacg ctccgccagc gcctgttcgg tggaaccgag gcccggctcg
     2341 cccaggcgcg ggcgagggct cgctccgccg atgcgcgcgc atcgagggtg cgggacgagg
     2401 cgtcgcgaga cgcggcgatc gcctggtcgg acgtccaggc gctggaggag cagctgcggg
     2461 ccctggaggc gagctacctc gcaagccgcc agtcgcgcga cgtcctgatc gagcggttca
     2521 tcaactcgcg cggcaacctg ttcgacgtcg tcgcggccga ggacgcctat ttcgagaccg
     2581 cgaccgcctt catcaggacc ttgagcgagc tcgacgcgg
//
LOCUS       DASVQZ010000190         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_909624, whole genome shotgun sequence.
ACCESSION   DASVQZ010000190 DASVQZ010000000
VERSION     DASVQZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909624"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VF547_05265"
     CDS             <1..416
                     /locus_tag="VF547_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8262265.1"
                     /translation="SQIRGVELARLAGLKRTGVSLARIAPGRESFVYHAHAREEEWLY
                     ILAGRGTAEIDGSEHEVGPGDFMGFAAPSVGHHLRNTGEEELVYLMGGERLDCDVATF
                     PRLGKTMVRVGERVEIYDSAAARPFGPLEGDGGDG"
     gene            552..1064
                     /locus_tag="VF547_05270"
     CDS             552..1064
                     /locus_tag="VF547_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262266.1"
                     /translation="MLGFFPRPAPAGLVGWAARVERKVAVEDYEAAFPGRPDAVYRFA
                     PDGSVTRLRPDYHAGEDQIRKDLSAPVLVFDPEAAWYFGDRPRLLPEPLMHLAASGQG
                     HRVNGRRPGDESALAAWLAEQGPPGILGRPRHGGSCAPCGGVRAAKASGDCRAQDRDA
                     PARGHGRALG"
     gene            1119..1283
                     /locus_tag="VF547_05275"
     CDS             1119..1283
                     /locus_tag="VF547_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8262267.1"
                     /translation="MQDLRKLLRDASGATAIEYGLLAALISIALVAAFNRLGLSLTGI
                     FETIAAALGG"
     gene            1360..>1552
                     /locus_tag="VF547_05280"
     CDS             1360..>1552
                     /locus_tag="VF547_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262268.1"
                     /translation="MARSPLAPLLLLPLAFAAAACTERRAGNASADMAGNAAGAVVAG
                     NAAAADSAFAPEKPAGTGKA"
BASE COUNT          211 a          531 c          601 g          209 t
ORIGIN      
        1 ggtcgcagat ccgcggagtc gagctggcgc ggctggcggg gttgaagcgg accggggtca
       61 gcctggcccg aatcgctccc ggccgggaga gcttcgtcta ccacgcccat gcccgcgagg
      121 aggaatggct gtacatcctc gccgggcgcg ggacggcgga gatcgacggg tcggagcatg
      181 aggtcgggcc gggcgacttc atgggcttcg ccgcgccctc ggtcgggcac catctgagga
      241 atacgggcga ggaggagctg gtctatctga tgggcggcga gcggctcgac tgcgacgtgg
      301 cgaccttccc gaggctcggc aagacgatgg tgcgggtggg agagagggtc gagatctacg
      361 acagcgccgc cgcgcggccg ttcgggccgc tcgaggggga cggcggtgac ggctgaggag
      421 gggcctcggc tctaccgcta cgtccatgtc agcgacgacg gcatggcgcc gtgcaccgcc
      481 ggcggccgac tcaccctggc gacctgcaag ccgcagatac gcggcccggc ccggccaggc
      541 cgggcgactg ggtgctcgga ttcttcccga ggccggcgcc ggcgggcctg gtcggctggg
      601 cggcccgggt cgagcgcaag gtggcggtgg aggattatga agcggcgttc ccgggccggc
      661 cggacgccgt ctaccgattc gcaccggacg gttcggtcac ccgccttcgt cccgactacc
      721 acgccggcga ggaccagatc cgcaaggacc tgtcggcccc ggtcctggtc ttcgacccgg
      781 aggccgcctg gtatttcggc gaccggccta ggctgctgcc cgagccgctg atgcatctgg
      841 cggcgagcgg ccagggccac cgcgtcaacg gccgccgccc gggcgacgag tcggctctgg
      901 cggcgtggct ggcggagcag gggccgcccg ggatcctcgg ccggccgcgg cacggcgggt
      961 cctgcgcgcc ctgcggcgga gtccgcgcgg cgaaggcgag cggcgactgc agggctcagg
     1021 atcgggacgc gccggcgcgc ggccacggcc gagctctggg ttaagcaagg cgtaaccttc
     1081 cccggccata gccaagggat cggggaggag cgaggaagtt gcaggatctc aggaaactgc
     1141 ttcgcgacgc gagcggcgcg acggcgatcg aatacggcct gctggcggcc ctgatctcga
     1201 tcgcgctggt ggccgcgttc aaccggctcg gcctcagcct caccggaata ttcgagacga
     1261 tcgcggccgc gctgggcggc tgagcggtcc atttcgggcg cgaaaggcgt tgtgcgcgcg
     1321 ggcgaagcat gaccatcttc gcccgaggag gatatcgcca tggcccggtc cccgctcgca
     1381 ccgcttctgc tgctcccgct cgctttcgcc gccgccgcct gtaccgagcg ccgcgccggc
     1441 aatgcctcgg ccgacatggc cggcaacgcc gccggggcgg tggtggccgg caatgccgcc
     1501 gccgccgatt ccgccttcgc cccggagaag ccggccggca ccggcaaggc cg
//
LOCUS       DASVQZ010000191         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_522470, whole genome shotgun sequence.
ACCESSION   DASVQZ010000191 DASVQZ010000000
VERSION     DASVQZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522470"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF547_05285"
     CDS             <1..285
                     /locus_tag="VF547_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX8262269.1"
                     /translation="AARPGQGENLWMGTRAAYELEEMAGAWAAEKSLFRPGIFPDVAR
                     RGKWSDVAHYTQMIWKGTRRVGCAVHSSPQWDFLVCRYSPPGNVVGQRVP"
     gene            467..1870
                     /locus_tag="VF547_05290"
     CDS             467..1870
                     /locus_tag="VF547_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8262270.1"
                     /translation="MNLFARKAVVADEDMAPEHRLHRSLGWPHLIALGVGAIVGTGIL
                     TLIGVGAAKAGPSVILSFAIAGLICACAALAYAEMATMIPASGSAYTYSYVVIGELIA
                     WIVGWSLILEYSLVVSAVAVGWSGYAAPLLHEAFGYPMALAQGPEIEGGLVNLPAIFI
                     IAVVAGLLIYGTKESATINALLVLVKIVALIVFVAVALPYFDAAHFEPFMPHGFPKSG
                     PPGAEVGVMAAAAIIFFAFYGFDAIATAAEEAKNPGRDLSIGIVGSMLACVIIYMAVA
                     AAAIGALSYTRFANSPEPLALILRELDQPWAARYLAASAVIALPTVILAFFYGQSRIF
                     FVMARDGLLPRSLARVSGRGSPVRITLFTAVIVAALAGIVPLDELAALANAGTLTAFI
                     AVCVCMLIMRRREPNAKRRFSTPLPWVVGIGGILGCLYLLYSLPWATQRYFMIAHVVG
                     LAIYFAYGARRSVAGRA"
     gene            complement(1956..2579)
                     /locus_tag="VF547_05295"
     CDS             complement(1956..2579)
                     /locus_tag="VF547_05295"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8262271.1"
                     /translation="MVGITDLIDGYRRFRESGWARQRERWSKLAEGQNPRVMVIACSD
                     SRVDPTQIFDAGPGEMFVVRNVAALVPPYETTGGHHGVSAALEFAVTQLQVEEVVVMG
                     HGFCGGCAAALTGQFDDAAHGEGHFIADWIELLRAARDKVRAEHGQDFRAMELEGVRV
                     SLANLRTFPWVREREADGRLTLHGAYFAIADGVLHLLDEASGEFAPA"
     gene            2729..>3143
                     /locus_tag="VF547_05300"
     CDS             2729..>3143
                     /locus_tag="VF547_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8262272.1"
                     /translation="MSEADPIRVPRQRKPDWIRVKAPTSAGFAETKRLMRSLNLATVC
                     EEAACPNIGECWTKKHATVMILGDTCTRACAFCNVKTGMPRAVDPLEPEHVATAAAEL
                     GLTHIVVTSVDRDDLPDGGAKQFVRVIEALRRTTPS"
BASE COUNT          463 a         1131 c         1032 g          517 t
ORIGIN      
        1 gccgcgcgcc ccggccaggg cgagaatctg tggatgggca cgcgcgccgc ctacgagctc
       61 gaggagatgg ccggcgcctg ggcggccgag aagagcctgt tccgccccgg aatcttcccg
      121 gacgtggccc ggcgcggcaa gtggagcgac gtcgcccact acacccagat gatctggaag
      181 ggcacgcgac gggtcggctg cgcggtccat tccagcccgc aatgggattt cctcgtctgc
      241 cgctactccc cgcccggcaa cgtggtcggc caaagggtgc cgtgatcctc cccatcgcca
      301 gatggggagg gggaccaccc gaaggtggag gggtgattga cggagggcct cgccggatac
      361 ccctccacca tgcttcgcat cgccccctcg ccaccgcttc gcggtcggga ggactaggct
      421 tggccgtgcg gcttgcctat caagcaaaaa aaagaagggg tgcctcatga acctgttcgc
      481 gcgcaaggcg gtggtcgccg acgaggacat ggcgccggag catcggcttc accggagcct
      541 cggctggccg catctgatcg cgctcggggt cggagcgatc gtcggcaccg gaatcctcac
      601 cctcatcggg gtgggcgccg ccaaggcggg tccctcggtg atcctctcct tcgccatcgc
      661 cgggctgatc tgcgcctgcg cggcgctcgc ttatgccgag atggcgacga tgatccccgc
      721 ctcgggaagc gcctatacct attcctacgt cgtcattgga gagctgatcg cctggatcgt
      781 cggctggagc ctgatcctcg aatattcgct ggtcgtgagc gccgtcgcgg tcggctggtc
      841 gggctatgcg gcccctttgc tccacgaggc gttcggctat cccatggcgc tggcgcaggg
      901 gccggagatc gaagggggcc tcgttaacct tccggccatc ttcatcatcg ccgtcgtcgc
      961 cgggctgctc atctacggca ccaaggagag cgcgacgatc aacgccctgc tcgtcctggt
     1021 gaagatcgtc gccctgatcg tgttcgtggc ggtggcgctc ccttatttcg acgccgccca
     1081 tttcgagccg ttcatgcccc acggcttccc caagagcggc cctcccggag cggaagtggg
     1141 cgtgatggcc gcggcagcga ttatcttctt cgccttttac ggcttcgacg cgatcgccac
     1201 ggccgccgag gaagccaaga atccgggccg cgacctctcc atcggcatcg tcggctcgat
     1261 gctcgcctgc gtgatcatct acatggccgt cgccgccgca gcgatcggag cgctgtccta
     1321 tacccgcttc gccaacagcc ccgagccgct cgccctcatc ctccgcgagc tcgaccagcc
     1381 ctgggccgcc cgctacctcg ccgcctcggc ggtgatcgcg cttccgaccg tcatcctcgc
     1441 cttcttctac ggccagagcc ggatcttctt cgtcatggcc cgcgacggcc ttctgccgcg
     1501 aagcctggcc cgggtgtcgg ggcgcggctc gccggtgcgg atcaccctgt tcacggcggt
     1561 gatcgtcgcc gcgctcgccg ggatcgtccc gcttgacgag ctcgccgccc tggccaatgc
     1621 cgggaccttg accgcgttca tcgcggtctg cgtctgcatg ctgatcatgc ggcggcgcga
     1681 gccgaacgcc aagcgccggt tcagcacgcc gctgccctgg gtggtgggga tcggcgggat
     1741 cctcggctgc ctctatctgc tctacagcct gccctgggcg acccagcgct atttcatgat
     1801 cgcccacgtc gtggggctcg ccatctattt cgcctacggc gcgcggcgca gcgtggcggg
     1861 ccgcgcttag cgtcattgcg aggagcgaag cgacgaagca atccagcggg cctccgacgc
     1921 cgtgctggat tgcttcgctt cgctcgcaat gacggctagg cgggagcgaa ctcgccgctc
     1981 gcctcgtcga gcaggtggag gaccccgtcg gcgatcgcga aataggcgcc gtggagcgtg
     2041 agccgaccgt cggcctcgcg ctcccgcacc caggggaacg tgcgcaggtt ggcgaggctg
     2101 acccggactc cctccagctc catcgcccga aaatcctggc cgtgctcggc gcgcaccttg
     2161 tcgcgggccg cgcgcagcag ctcgatccag tcggcgatga aatggccctc gccgtgggcg
     2221 gcgtcgtcga actggccggt cagcgccgcc gcgcagccgc cgcagaagcc gtggcccatc
     2281 accaccacct cctcgacctg gagctgggtg accgcgaact cgagcgcggc cgacacgccg
     2341 tgatggccgc cggtcgtctc gtagggcggc accagagcgg cgacgttcct caccacgaac
     2401 atctcgcccg gtcccgcgtc gaagatctgg gtcgggtcga cccggctgtc cgagcaggcg
     2461 atgaccatca cccgcgggtt ctggccctcg gcgagcttcg accagcgctc gcgctggcgc
     2521 gcccagccgc tctcgcggaa gcggcggtag ccgtcgatga ggtccgtgat cccgaccaag
     2581 cccaaatcct ccatccgttc gggctgagcc tgtcgaagcc ctccctcgct tgccgcgccc
     2641 taagagaagc ggaggccttc gacaagctca ggccgaccgg ggtgggggag gggtggcaag
     2701 tcaggtcaac catcgctatc tggccggcat gagcgaagcc gacccgatcc gagtcccgag
     2761 gcagcgcaag cccgactgga tccgggtcaa ggccccgacc agcgccggct tcgccgagac
     2821 caagcggctg atgcgcagcc tcaacctcgc caccgtctgc gaggaggcgg cctgccccaa
     2881 tatcggcgag tgctggacca agaagcacgc gacggtgatg atcctcggcg acacctgcac
     2941 ccgcgcctgc gccttctgca acgtcaagac cggcatgccg agggcggtcg acccgctcga
     3001 gcccgagcat gtcgcgaccg cggcggccga gctcggcctc acccacatcg tcgtcacctc
     3061 ggtcgaccgc gacgaccttc ccgacggcgg ggcgaagcag ttcgttcggg tgatcgaggc
     3121 gcttcgccgg acgaccccct cga
//
LOCUS       DASVQZ010000192         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_812352, whole genome shotgun sequence.
ACCESSION   DASVQZ010000192 DASVQZ010000000
VERSION     DASVQZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812352"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VF547_05305"
     CDS             complement(<1..764)
                     /locus_tag="VF547_05305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8262273.1"
                     /translation="MSSPKLDWRCKGLPARAEGLTGAEIGALGLSLTGGDMMMPVAVL
                     RERALRHNMAAMQAFADGTGAHLAPHGKTSMSPELFAMQMAAGAWGLTAATAHHVRVY
                     RRFGVRRVFLANQLVAPADIAWILAEIARDRNFDFYCLVDSPESVGLLASAARAAGTP
                     RPVQVLVETGLPGGRTGARDHSAAMKVARIVAGASPWLALRGVETYEGIRQSRDGARQ
                     EAAAMIALAVSVAEAIAGEGLLADGPLLLSAGGSAFL"
     gene            complement(786..1856)
                     /gene="hppD"
                     /locus_tag="VF547_05310"
     CDS             complement(786..1856)
                     /gene="hppD"
                     /locus_tag="VF547_05310"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX8262274.1"
                     /translation="MTPVPKDNPLGLDGFEFVEFTSPDPEAMADLFVKLGFVHAGNHR
                     RIDVRHYAQGDINFLLNMEAVGHADEFRRAHGPSASAMAFRVRDSREALRMAVERGAV
                     AAEGAYGYPAIEGIGGSLLYLVDRYGAETLYDSDFAPVGDGARNPVGAGLHTLDHLTH
                     NVFRGRMNHWAQFYERIFNFREIRYFDIEGQQTALLSRAMTAPDDKIRIPLNESQDEV
                     SQIAEYLRDYNGEGIQHIALATDDIFATVDTLRAAGIRFQDSPETYYDMIDERLPGHD
                     HDIEQMRKRRILIDGSPETGEGLLLQIFTENMIGPIFFEIIQRKGNQGFGEGNFKALF
                     ESIELDQVRRGVLPAAAPAGAR"
     gene            1999..>2346
                     /locus_tag="VF547_05315"
     CDS             1999..>2346
                     /locus_tag="VF547_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8262275.1"
                     /translation="MAATHSTRMLILGSGPAGLTAAIYGARAGLAPIVVQGMQPGGQL
                     TITTDVENYPGFADVIQGPWLMEQMQKQAEHVGARMMWDQIVEVDLTQRPFRLIGDGG
                     TVYSGDTLVIATGA"
BASE COUNT          357 a          770 c          837 g          382 t
ORIGIN      
        1 agaaaggcgg agccgccggc ggagagcagg agcgggccgt cggccagcag tccctctccc
       61 gcgatcgcct cggcgacgga gacggcgagg gcgatcatgg ccgccgcctc ctggcgtgct
      121 ccgtcgcggc tctggcggat gccttcgtag gtttcgacgc cgcgcagcgc gagccatggg
      181 gaggcgccgg caacgatgcg ggcgaccttc attgcggcgc tgtggtcgcg ggcgccggtg
      241 cggccgccgg gcaggccggt ctcgacgagc acctgaaccg gccgcggcgt cccggccgca
      301 cgcgcagccg aggcgaggag tccgacgctc tccggcgagt ccacaaggca gtagaagtcg
      361 aagttccggt cgcgagcaat ctccgcgaga atccaggcaa tatcagcggg cgcgacgagc
      421 tggttggcga ggaagacgcg ccggacgccg aagcggcggt acacccggac atggtgcgcg
      481 gtcgccgccg tcagccccca ggcgcccgcc gccatctgca tggcgaagag ttcggggctc
      541 atgctggtct tgccgtgtgg ggcgaggtgc gcgccggttc cgtcggcgaa ggcctgcatc
      601 gccgccatat tatggcgcag cgcccgctcg cgcagcacgg cgacgggcat catcatgtcg
      661 ccgccggtga ggctcagccc cagcgctccg atctcggctc ccgtcagccc ctccgcccgc
      721 gccggcaatc ccttgcagcg ccagtccagc ttgggcgaac tcaagaggcc cgccccgcct
      781 taaacttagc gcgcgcccgc tggcgccgcc gcggggagga cgccgcggcg gacctggtcg
      841 agttcgatcg attcgaagag cgccttgaaa ttgccctcgc cgaagccctg gtttcccttc
      901 ctctggatga tttcgaagaa gatcggaccg atcatgttct cggtgaagat ctggaggagg
      961 agaccttcgc cggtctcggg cgagccgtcg atcaggatgc ggcgcttcct catctgctcg
     1021 atgtcgtggt cgtggccggg caggcgctcg tcgatcatgt cgtaataggt ttcggggctg
     1081 tcctggaagc ggatgccggc ggcgcgcagc gtgtcgacgg tggcgaagat gtcgtcggtc
     1141 gcgagcgcga tgtgctggat gccctcgcca ttatagtcgc gcagatattc cgcgatctgc
     1201 gagacttcgt cctggctctc gttcaatgga atgcggatct tgtcgtccgg cgccgtcatg
     1261 gcgcgggaga ggagggcggt ctgctggccc tcgatgtcga aatagcggat ttcgcggaag
     1321 ttgaagatcc gctcgtagaa ctgggcccag tggttcattc ggccgcgaaa cacattgtgg
     1381 gtgaggtggt cgagcgtgtg gagcccggcg cccaccggat tgcgcgcgcc gtcgcccacg
     1441 ggcgcaaagt cgctgtcgta cagcgtctcg gcgccgtagc ggtccacgag gtagaggagg
     1501 gagccgccga tcccctcgat tgccggatag ccgtaggcgc cttcggccgc gaccgcgccg
     1561 cgctcgacgg ccatgcgcag ggcctcgcgc gagtcccgga cgcgaaaggc catggcgctc
     1621 gccgagggac cgtgcgcgcg gcggaattcg tcggcgtggc cgacggcctc catgttgagg
     1681 aggaagttga tatcgccctg tgcataatgg cgcacgtcga tgcgccggtg gttgccggcg
     1741 tggacgaagc ccagcttcac gaagagatcg gccatcgcct cgggatcggg gctggtgaat
     1801 tcgacgaatt cgaagccgtc gaggccgagc gggttgtcct tgggaacggg ggtcatggcg
     1861 ctggctccgc gtagatggtg acgtgtcgcg cgggccttac cagcgccggc ggggagcggc
     1921 aaatggatgg ttgcgcgggc gcgccgggct gcttacctcc ccagtctcac ctcacatcaa
     1981 caagcgagac gccccgcgat ggccgcgacc cattccaccc gcatgctgat cctcggctcg
     2041 ggccctgccg gcctcaccgc cgccatctac ggcgcccgtg ccggcctcgc cccgatcgtc
     2101 gtccagggca tgcagcccgg cggccagctc accatcacca ccgacgtcga gaattatccc
     2161 ggcttcgcgg acgtgatcca gggcccgtgg ctgatggagc agatgcagaa gcaggccgag
     2221 catgtcggcg cgcgtatgat gtgggaccag atcgtcgagg tcgacctgac gcagcggccc
     2281 ttccggctga tcggcgacgg cggcaccgtc tattcggggg atacgctcgt catcgccacc
     2341 ggcgcc
//
LOCUS       DASVQZ010000193         7373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_142644, whole genome shotgun sequence.
ACCESSION   DASVQZ010000193 DASVQZ010000000
VERSION     DASVQZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142644"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VF547_05320"
     CDS             <1..654
                     /locus_tag="VF547_05320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951469.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX8262276.1"
                     /translation="AYPGDVFYLHSRLLERAAKLNEDNGSGSLTALPIIETQAGDVSA
                     YIPTNVISITDGQIFLETELFYQGIRPAINVGLSVSRVGSAAQTKAMKKVAGSIKLEL
                     AQYREMAAFAQFGSDLDASTQRLLNRGARLTELLKQPQFRPMPVEEQVVSIYAGVNGF
                     IDNVPVNAVTRFETALLSHFRSDHADVLGKIRETKTLDDDTAGRLKTIIGDFAKSFA"
     gene            768..1649
                     /locus_tag="VF547_05325"
     CDS             768..1649
                     /locus_tag="VF547_05325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX8262277.1"
                     /translation="MASLKALKGRIASVKSTQKITKAMKMVAAAKLRRAQQAAEAGRP
                     YAERMENVMASLASKVAISPQSPKLLAGTGHDQTHLLVVCTSDRGLAGAFNSNIVRAA
                     RKKADDLTRQGKQVLFYLVGRKGRAVIQRLYPRQIIHQHDTSAMKFPSFADALAIAND
                     LIDRYQTDGIDVVHLFYAHFQSALVQEPVAQQIIPVSIPEGGKATTGANAAIEYEPDE
                     EDILAALLPRNVAVQIFRALLENAASEQGSRMTAMDNATRNAGDMIQRLTIVYNRTRQ
                     AAITTELVEIISGAEAL"
     gene            1736..3199
                     /gene="atpD"
                     /locus_tag="VF547_05330"
     CDS             1736..3199
                     /gene="atpD"
                     /locus_tag="VF547_05330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX8262278.1"
                     /translation="MATAATATPTNNIGRISQVIGAVVDVTFDSDLPEILSALETDNN
                     GNRLVLEVAQHLGESTVRTIAMDSTDGLTRGQPVTDTGSQIRVPVGPKTLGRIMNVVG
                     EPIDERGPIGATETAPIHAEAPLFVDQSTDNAILVTGIKVIDLLAPYAKGGKIGLFGG
                     AGVGKTVLIQELINNIAKGHGGTSVFAGVGERTREGNDLYHEFLEAGVIAKDADGNAI
                     SEGSKVALVFGQMNEPPGARARVALSGLTQAEYFRDVEGQDVLFFVDNIFRFTQAGSE
                     VSALLGRIPSAVGYQPTLSTDMGALQERITSTNKGSITSVQAIYVPADDLTDPAPATS
                     FAHLDATTVLSRAISELGIYPAVDPLDSTSRVLEPRTVGQEHYETARAVQEILQRYKS
                     LQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFHVAEVFTGIPGKFVQVEDTVRSF
                     KAVVDGEYDHLPEAAFYMVGGIEEAVEKAQRLAAEAA"
     gene            3207..3743
                     /locus_tag="VF547_05335"
     CDS             3207..3743
                     /locus_tag="VF547_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262279.1"
                     /translation="MSSAPALFLLLAAPAAPQPTMVDAARQALMEGCVPHTIARRTIE
                     KKNAAPLVAAGIKVGAPIPDWARTGLEEWGASVPAEVASAEGPVWISAFKAGICSVMF
                     TSGERHSANAEALRRMIEAPGSGFSKVEDKTYGDTVTRFYDSPLKPRGTLRLTFSATN
                     EAAPKGGEMIIASTSRID"
     gene            3758..4027
                     /locus_tag="VF547_05340"
     CDS             3758..4027
                     /locus_tag="VF547_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX8262280.1"
                     /translation="MADLHFELVTPERLIRSEEVFMVVVPGSEGDFGVLAGHAPYMST
                     LRDGDIAVYRTGFADAPERIAVEGGFAEVSERGLTVLAEKTDVPA"
     gene            complement(4058..4525)
                     /locus_tag="VF547_05345"
     CDS             complement(4058..4525)
                     /locus_tag="VF547_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX8262281.1"
                     /translation="MKRRTRLVGLFAVAAATVVTPAPAKGRAGIDQAGWLAGVWVTRS
                     GGRWTEERWAPPRGGVMLGTSLSGEGAAASSYEYMRIAVADDGKLTFWGSPEGRPPVA
                     FRLARAGPNLLEFENAAHDYPTRISYRREGEQLLATISGPNGANPMSWRYKRR"
     gene            4614..5333
                     /locus_tag="VF547_05350"
     CDS             4614..5333
                     /locus_tag="VF547_05350"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8262282.1"
                     /translation="MDYQEIDPPEPLRPLVKAGWTLDAAGDPGALLRHSATPDGCIEI
                     IRRLSGRSAWGGAQPDGFVAGLVTAPAELELSGDARFVGLRLWPWAWNAMAHIPSPAL
                     IDRWRPIAEAAPGFVMPATIESAFARIARHALDPAVAALGEAILESRTVEALSAASGR
                     PRRWLQRWFEREIGVPPRRYLRLLRFQEALTGLQASEDSLARQAAEHGFADQAHMARE
                     FRAIARAPAGTARRTAKGPFL"
     gene            complement(5330..6229)
                     /locus_tag="VF547_05355"
     CDS             complement(5330..6229)
                     /locus_tag="VF547_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8262283.1"
                     /translation="MSQAPARAALVLALAGFCSLAVGDAVVKSMAGDWPAPAVSALRY
                     CFGAIGLGLFVAWRFGRAGFVMPRPGLQLGRGAAVSLATLCFFMAVMAMPLADATAIQ
                     FTSPILTALLAPLVVGERTRPATWAATLLAFAGVLVVLRPNLVEIGFVALFPLGAAFG
                     MSWLMMLNRMTAGDAPVMVMQFLLGIIAAPALVAAAAALHFAGLVPIGSPSPAVVLKC
                     LGVAVFATLGHSLIFAAVERANASVVAPMTYVQLIVAAGLGWLWFGDSPDAATFGGAA
                     LIIAGGLLLWRAQRVRDLGEAPA"
     gene            6433..>7373
                     /locus_tag="VF547_05360"
     CDS             6433..>7373
                     /locus_tag="VF547_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX8262284.1"
                     /translation="MSAFGRRGGNGGPAGGRPSFGIASPMKGGPGARPAPLAMPEGGD
                     QFPPLDSIEFSDSDTDGFVPAVTDRGADAMSRLADRQAASSEAPSSKNEGFEASIHRI
                     KEQVLPRLLERVDPEAAATLSKDELAEEFRPIIGEVLAELKINLNRREQFALEKVLVD
                     ELLGLGPLEELLADPAISDIMVNGPNQTFIEKKGKLEIAQIQFRDEEHLFQIAQRIVS
                     KVGRRVDQTTPLADARLMDGSRVNVIVPPLSLRGTAISIRKFSAKPITLDMMTGFGSM
                     DAKMATLLKIAGASRMNIIISGGTGSGKTTMLNALSK"
BASE COUNT         1275 a         2675 c         2389 g         1034 t
ORIGIN      
        1 gcctatccgg gcgacgtctt ctacctccac tcgcggctgc tcgagcgcgc tgcgaagctc
       61 aacgaggaca atggctcggg ctcgctcacc gctcttccga tcatcgagac ccaggcgggc
      121 gacgtgtcgg cctatatccc gaccaacgtc atctcgatca ccgacggcca gatcttcctc
      181 gagaccgagc tcttctacca gggcatccga ccggcgatca acgtcggcct ctccgtgtcc
      241 cgcgtcggct cggccgcgca gacgaaggcg atgaagaagg tcgccggctc gatcaagctc
      301 gagctcgccc agtatcgcga gatggccgcc ttcgcccagt tcggctccga cctcgacgcc
      361 tcgacccagc gcctgctcaa ccgcggcgcg aggctgaccg agctcctcaa gcagccgcaa
      421 ttccggccga tgccggtcga ggagcaggtc gtctccatct acgcgggcgt caacggcttc
      481 atcgacaacg ttccggtcaa cgccgtgacc cgcttcgaga ccgcgctcct cagccacttc
      541 cgcagcgatc atgccgacgt gctcggcaag atccgcgaaa ccaagacgct ggacgacgac
      601 acggcgggcc ggctgaagac catcatcggc gatttcgcga agtcgtttgc gtaacacccg
      661 caccgccgtc accccagcga aggctggggt ccaagcggcg gtggggacac gaagacagca
      721 tggatgccag ccttcgctgg catgacgaag gactgaagta ggaagccatg gcctctctca
      781 aggcgctcaa gggcaggatc gcgtcggtca agtcgacgca gaagatcacc aaggcgatga
      841 agatggtcgc cgccgcgaag ctgcgccggg cgcagcaggc ggcggaggcc ggccgtccct
      901 acgccgagcg catggaaaac gtgatggcga gtctcgcctc caaggtcgcg atcagcccgc
      961 agagcccgaa gctgctcgcg ggcaccgggc acgaccagac ccatctgctc gtcgtctgca
     1021 cctccgaccg aggccttgcc ggagcgttca actcgaacat cgtccgagcc gcccgcaaga
     1081 aggccgacga cctcacccgc cagggcaagc aggtcctgtt ctacctggtc ggccgcaagg
     1141 gccgagcggt gatccagcgc ctctacccga ggcagatcat ccaccagcac gacaccagcg
     1201 cgatgaagtt cccgagcttc gccgacgctc tggcgatcgc caacgacctg atcgaccgct
     1261 accagaccga cggaatcgac gtcgtccacc tcttctacgc ccatttccag tcggccctgg
     1321 tgcaggagcc ggtggcgcag cagatcatcc cggtctcgat cccggagggc ggcaaggcga
     1381 cgaccggcgc gaacgccgcc atcgaatatg agcccgacga ggaggacatc ctcgccgcgc
     1441 tcctgccccg caacgtcgcc gtccagatct tccgcgccct gctggagaac gcggcatccg
     1501 agcagggcag ccggatgacc gcgatggaca acgccacccg caacgccggc gacatgatcc
     1561 agcgcctgac catcgtctac aaccggacga ggcaggccgc gatcaccacc gagctcgtgg
     1621 agattatttc gggggccgag gccctgtagt cagctgctcc ccggccaagg ccggggccca
     1681 gcgaagttag aaactgggcc ccggccttcg ccggggaaca agaaggaaga gaagaatggc
     1741 caccgccgca accgccacgc ccaccaacaa tatcggccga atcagccagg tgatcggcgc
     1801 cgtcgtcgac gtgacgttcg acagcgacct gcctgagatc ctgtcggcgc tcgagaccga
     1861 caataacggc aatcgcctcg tgctcgaagt cgcccagcat ctcggcgagt cgaccgtgcg
     1921 cacgatcgcg atggactcga ccgacggcct cacccgcggc cagcccgtga ccgacacggg
     1981 ctcgcagatc cgcgtccccg tcggtcccaa gacgctcggc cgcatcatga acgtggtcgg
     2041 cgagccgatc gacgagcgcg gcccgatcgg cgccaccgag accgctccca tccacgccga
     2101 ggccccgctg ttcgtcgacc agtcgaccga caacgcgatc ctcgtcaccg ggatcaaggt
     2161 catcgacctg ctcgccccct acgccaaggg cggcaagatc ggcctgttcg ggggcgccgg
     2221 ggtcggcaag accgtgctga tccaggagct gatcaacaac atcgccaagg gccatggcgg
     2281 gacctcggtg ttcgccgggg tcggcgagcg cacccgcgag ggcaacgacc tctaccatga
     2341 gttcctcgag gccggggtca tcgccaagga cgcggacggc aacgccatct cggaaggctc
     2401 gaaggtcgcg ctcgtcttcg gccagatgaa cgagccgccg ggcgcccgcg cccgcgtcgc
     2461 gctgtccggg ctcacccagg ccgaatattt ccgggacgtc gaaggccagg acgtgctctt
     2521 cttcgtcgac aacatcttcc gcttcaccca ggccggctcg gaagtgtcgg cgctgctcgg
     2581 ccgaatcccc tcggcggtgg gctaccagcc gaccctgtcg accgatatgg gcgcccttca
     2641 ggagcgcatc acctcgacca acaagggctc gatcacctcg gtgcaggcca tctacgtgcc
     2701 cgccgacgat ctcaccgatc cggcgccggc cacgagcttc gcccacctcg acgccaccac
     2761 cgtgctgtcg cgcgccatct cggagctcgg catctacccg gccgtggacc cgctcgactc
     2821 cacctcgcgc gtgctcgagc cgcgcaccgt cggccaggag cattacgaga ccgcccgagc
     2881 cgtccaggag atcctccagc gctacaagtc gctccaggac atcatcgcca tcctcggcat
     2941 ggacgagctc agcgaggagg ataagctgac ggtcagccgc gcccgcaaga tccagcgctt
     3001 cctctcgcag ccgttccacg tcgccgaggt gttcaccgga atccccggca agttcgtcca
     3061 ggtcgaggac acggtccgca gcttcaaggc ggtcgtcgac ggcgaatatg accacctgcc
     3121 cgaggccgcc ttctacatgg tcggcggcat cgaggaagcc gtcgagaagg cccagcgcct
     3181 ggcggccgaa gcggcctgac gcccgcgtgt cgtccgcccc tgccctcttc ctgctcctcg
     3241 ccgcgccggc ggcgccgcag ccgaccatgg tcgacgcggc gcgccaggcg ctgatggagg
     3301 gctgcgtgcc gcataccatc gcccggcgga cgatcgagaa gaagaatgcg gcaccgcttg
     3361 tcgccgctgg catcaaggtg ggcgccccga tccccgactg ggccaggacc ggcctcgagg
     3421 aatggggggc gtccgtcccc gccgaagtcg cttcggccga ggggccggtc tggatcagcg
     3481 ccttcaaggc aggcatttgc tcggtcatgt tcactagcgg cgagcgccat tcggccaacg
     3541 ccgaggcgtt gcgacggatg atcgaggcgc cgggaagcgg gttcagcaag gtcgaggata
     3601 agacgtacgg ggacaccgtc acccgcttct acgactcccc gctgaagccg cgcggaactc
     3661 ttcgccttac cttcagcgca accaacgaag ccgctccgaa aggcggtgag atgatcatcg
     3721 cctcgacctc ccgaatcgat tgaactggac cgcgaacatg gccgacctgc acttcgaact
     3781 cgtcacgccc gagcggctga tccgctccga ggaagtcttc atggtcgtgg tgccgggcag
     3841 cgagggcgat ttcggggtcc tcgccggcca cgcgccctac atgtcgacgc tccgggacgg
     3901 cgacatcgcg gtctaccgca caggcttcgc cgacgcgccg gagcggatag cggtcgaggg
     3961 cggcttcgcc gaggtcagcg agcggggcct gaccgtgctc gccgagaaga ccgacgtccc
     4021 cgcctgagcg gcacccccgc cgccgaggcg gcaggattca ccgtcttttg tagcgccagc
     4081 tcatcgggtt cgcgccgttc ggaccggaga tggtcgcgag cagctgctcg ccctcgcgcc
     4141 gatagctgat cctggtcgga taatcgtggg ccgcgttttc gaactcaagc aggttcggac
     4201 ctgccctcgc caggcgaaac gccacgggcg gcctgccctc gggcgagccc cagaaggtga
     4261 gcttgccgtc gtcggcaacg gcgatccgca tatattcata ggaggaagcg gccgccccct
     4321 cgccgctgag gctggtgccg agcatgaccc cgccgcgggg cggggcccat ctctcctcgg
     4381 tccagcggcc tcccgaccgg gttacccaga cgccggcgag ccaccccgcc tggtcgattc
     4441 cggctcggcc cttggccggg gccggcgtga cgaccgtcgc cgccgcgacg gcgaacagcc
     4501 cgacaagcct ggtcctgcgc ttcatcgacg tcctccccat gcgctcgccc ctcttctatc
     4561 ccttttccgt tcggccggct tgaacgaacg cgacagcctg ccatgctggc tgggtggact
     4621 atcaggagat cgacccgccc gaaccgcttc gcccactggt caaggcgggc tggaccctgg
     4681 acgccgccgg cgaccccgga gctctccttc gccactcggc gacgccggac ggatgcatcg
     4741 agatcatccg ccgcctcagc ggccgctccg cctggggcgg cgcccagccc gacggcttcg
     4801 tcgccgggct cgtcacggca ccggccgagc tcgagctcag cggcgatgcg cgcttcgtcg
     4861 ggcttcggct gtggccctgg gcgtggaacg cgatggccca tatcccttcg cccgcgctga
     4921 tcgaccgctg gcggccgatc gccgaggcgg cccccggctt cgtcatgccc gcgaccatcg
     4981 aatcggcctt cgcgaggatc gctcgacacg ccctcgaccc ggccgtcgcg gcgctcggcg
     5041 aagccatcct cgagtcccgc accgtcgagg cgctgagcgc cgcctccggc cggccccggc
     5101 gctggctgca gcgttggttc gagcgcgaga tcggagtgcc gccgcggcgc tatctgcggc
     5161 tgctgcgctt ccaggaggcc ttgacgggat tgcaggcgtc ggaggacagc ctcgcccgcc
     5221 aggccgccga gcacggattc gccgaccagg cccatatggc ccgcgagttc cgcgccatcg
     5281 cccgcgcccc ggccgggacc gcgcggcgga cggccaaggg tcctttcctc taggccggtg
     5341 cctcgccgag atcgcgcacc cgctgcgcgc gccacaggag caggccgccg gcgatgatca
     5401 gcgcggcgcc gccgaacgtc gccgcgtccg gcgagtcgcc gaaccacagc cagcccaggc
     5461 ccgccgcgac gatcagctgg acgtaggtca tcggcgccac caccgaggcg ttggcgcgct
     5521 cgaccgccgc gaagatcagg gaatggccga gggtggcgaa gaccgcgacg ccgaggcact
     5581 tgagcaccac ggccggcgac ggcgatccga tcggaacgag gccggcgaag tgcagggccg
     5641 cggcggcggc gaccagcgcc ggagcggcga tgatgccgag gaggaactgc atcaccatca
     5701 cgggcgcgtc cccggccgtc atccggttca gcatcatcag ccacgacatg ccgaacgccg
     5761 cgccgagcgg gaacagcgcg acgaagccga tctcgaccag gttggggcga agcaccacca
     5821 gcaccccggc gaaggcgagc agggtcgccg cccaggtggc gggacgggtg cgctcgccga
     5881 cgaccagggg ggccagcaga gcggtgagga tcgggctcgt gaactggatg gcggtggcgt
     5941 cggcgagcgg catcgccatc accgccatga agaagcacaa ggtcgcgagc gacaccgcgg
     6001 cgccccggcc cagctgcagc cccggcctcg gcatcacgaa gccggcgcgc ccgaagcgcc
     6061 aggcgacgaa aaggccgagc ccgatggctc cgaagcaata gcgaagggcc gagacggccg
     6121 gggccggcca gtcgccggcc atcgatttta cgacggcgtc cccgaccgcg agcgagcaga
     6181 agcccgccag ggcgaggacc agggcggcgc gggccggggc ttggctcacc gccgccgctc
     6241 ccgctccccg gcgaaaaccg gagtccggcc tgggtcccgg gttccggcct tcgccgggac
     6301 gcatggctcg gcgattctgt tcagcatgac gacactcccg ctcctcgcca tgcggttagg
     6361 caattgtcaa agtttccggc ctattcacca cccgagagcg gcaagcttca ccggttgagg
     6421 gattaaagcg caatgagtgc ttttggacga cgcggaggaa atggcggccc ggcgggcggg
     6481 cgcccgagct tcggcatcgc gagcccgatg aagggtgggc cgggcgcccg gcccgcgcct
     6541 ctggcgatgc ccgaaggcgg cgaccaattc cctccgctcg actcgatcga attctccgac
     6601 tccgacaccg acggcttcgt cccggccgtt accgatcgcg gcgccgacgc catgtcccgc
     6661 ctcgccgacc gccaggcggc ctcgtcggaa gcgccgtcga gcaagaacga gggcttcgag
     6721 gcctcgatcc accgcatcaa ggagcaggtg ctcccccgcc tgctcgagcg cgtcgacccg
     6781 gaagcggcgg cgacgctgag caaggacgag ctcgccgagg agttccggcc gatcatcggc
     6841 gaggtgctcg ccgagctgaa gatcaacctc aaccgccgcg agcagttcgc gctcgagaag
     6901 gtgctggtcg acgagcttct cggcctcggc ccgctcgagg agctgctcgc cgatccggcc
     6961 atctccgaca tcatggtcaa cgggcccaac cagaccttca tcgagaagaa gggcaagctg
     7021 gagatcgccc agatccagtt ccgcgacgag gagcatctgt tccagatcgc gcagcggatc
     7081 gtctccaagg tcggccgccg cgtcgatcag acgacgccac tagccgatgc ccgcttgatg
     7141 gacggcagcc gcgtcaacgt gatcgtgccg ccgctctcgc tgcgcggcac cgccatctca
     7201 attcgtaaat tttccgccaa gccgatcacg ctcgacatga tgaccggctt cggatcgatg
     7261 gacgcgaaga tggcgacctt gctcaagatc gccggcgcca gccgaatgaa catcatcatc
     7321 tcgggcggca ccggctcggg taagacgacg atgctcaatg ccttgtcgaa gat
//
LOCUS       DASVQZ010000194         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_522694, whole genome shotgun sequence.
ACCESSION   DASVQZ010000194 DASVQZ010000000
VERSION     DASVQZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522694"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..439
                     /locus_tag="VF547_05365"
     CDS             134..439
                     /locus_tag="VF547_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262285.1"
                     /translation="MRSALLSALLLAGCTAQVSHPTKTLAEQQVDIDFCTEWANDKYW
                     MDPVAALLNAYDCLEAKGYRKERSGLKERVERASGEDRVRRRDPRDPTRPCTVPCRR"
     gene            complement(447..1100)
                     /locus_tag="VF547_05370"
     CDS             complement(447..1100)
                     /locus_tag="VF547_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX8262286.1"
                     /translation="MAGPRSLYDVLNVAPEAEPVVVEAAYKALIKKYHPDQAAEAPVS
                     KDAAAINEAFAVLKDPAKRADYDHRLWTKQQALRLAELQAGGGARRPRFAAAGGWIVA
                     GLLACALAAVALGKSLAPPPHSAAAQAAEPHDAEQPARAAAAKAAAAEALHDRVAMPS
                     SAAVIARVRAEREVRSFARAARAAPTPIRYRKARRAPAPSREPRREKDFLERQGYIY"
     gene            1242..1652
                     /locus_tag="VF547_05375"
     CDS             1242..1652
                     /locus_tag="VF547_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8262287.1"
                     /translation="MRDLGLPADWAPRLLSLLRIVAALIFLLHGAQKLLGFPPLAPGM
                     AMPEPFTLMWIGGVLELFGGLMLLIGLFTRPVAFVLSGEMAVAYWMFHAPQGPFPTLN
                     GGDAAILYCFAFLYLAAAGPGPWSVDALRRAGRR"
     gene            1653..>1969
                     /locus_tag="VF547_05380"
     CDS             1653..>1969
                     /locus_tag="VF547_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX8262288.1"
                     /translation="MADAPRQAGNIALLIDADNASSDHLDVVLTVLAELGTVNIRRAY
                     GNWSKSALKGWAEMTHRHAIEPHQQFDITKGKNATDMKMTIDAMDLLFSGRVEGFGIM
                     SSD"
BASE COUNT          284 a          704 c          701 g          280 t
ORIGIN      
        1 cggcccattc cggccgcgag ccgcagccgc ccagggccgt cgaggccgcc agggccatca
       61 gcgcgaaaag acgcatcgcc gactcctttg cccattaagt tgcgatccgc cgccgcggat
      121 gcaagaaagc gccatgaggt ccgcgttgct ttccgcactg ctgctcgccg gctgcacggc
      181 gcaggtctcg catccgacca agacgctggc cgagcagcag gtcgacatcg acttctgcac
      241 cgagtgggcg aacgacaaat attggatgga cccggtcgcc gccctgctca acgcctacga
      301 ctgcctcgag gcgaaaggct accggaagga gaggagcggg ctcaaggaac gggtcgagcg
      361 cgcctcgggg gaagaccggg tccgccgccg ggacccgcgc gatccgacgc ggccctgcac
      421 cgtcccctgc cgccgctgag gccggactag tagatgtagc cctgccgctc gaggaagtcc
      481 ttctcccggc gcggctcgcg cgaaggcgcc ggagcgcggc gggccttgcg gtagcggatc
      541 ggggtcgggg cggcgcgggc ggcgcgggcg aaggagcgga cctcgcgctc ggcccgcacc
      601 cgggcgatca ccgccgccga cgaaggcatg gcgactcgat cgtggagggc ctcggcggcc
      661 gcggccttgg cggcggcggc ccgggccggc tgctcggcat cgtgcggctc ggcggcctgg
      721 gcggcggcgc tgtgcggcgg cggcgcgagg ctcttgccga gcgcgaccgc ggccagggcg
      781 caggcgagca gcccggcgac gatccagcct ccggcggcgg cgaagcgcgg ccggcgggcg
      841 ccgccccccg cctggagctc ggcgaggcgc agcgcctgct gcttggtcca caggcgatgg
      901 tcgtaatcgg cgcgcttggc cggatccttg agcaccgcga aggcctcgtt gatcgccgcg
      961 gcgtccttcg agacgggcgc ctcggccgcc tggtcgggat gatatttctt gatcagcgcc
     1021 ttgtaggcgg cctcgaccac gacgggctcc gcttcgggag cgacgttgag gacgtcgtag
     1081 aggctgcgcg gaccggccat ggcagggaga cttctgccgc agaaagccgg aaagcgccag
     1141 agccgaagta atccagcccg cccaacggct tggccgatgc cgctttcgcc ccgcgcgcgg
     1201 cggtgttagg cacgtcgcgc ggagcggaag gagccggcct gatgcgtgac ctgggactgc
     1261 ccgcggactg ggcgccgcgc ctgctgagcc tgctcaggat cgtcgccgcg ctcatcttcc
     1321 tgcttcacgg cgcccagaag ctgctcggct tcccgcccct cgcgccggga atggcgatgc
     1381 cggagccgtt caccctgatg tggatcggcg gcgtgctcga gctgttcggc ggcctcatgc
     1441 tgctgatcgg cctgttcacc cggccggtcg cgttcgtcct ctccggcgag atggccgtcg
     1501 cctattggat gttccacgcg ccccagggcc ccttccccac gctcaacggc ggcgacgcgg
     1561 cgatcctcta ctgcttcgcc ttcctctatc tcgcggccgc cggcccgggg ccgtggagcg
     1621 tcgacgccct gcgccgcgca ggccgccgct agatggccga cgctccccgc caggccggca
     1681 acatcgcgct tctgatcgac gccgacaacg cctcgtcgga ccatctcgac gtcgtcctga
     1741 cagtgctcgc cgagctcggc acggtgaaca tccgccgcgc ctacggcaat tggagcaagt
     1801 cggcgctcaa gggctgggcg gagatgaccc accgccacgc gatcgagccc caccagcagt
     1861 tcgacatcac caagggcaag aacgccaccg acatgaagat gacgatcgat gcgatggacc
     1921 tgctgttcag cggccgggtc gagggcttcg gcatcatgtc gagcgacag
//
LOCUS       DASVQZ010000195         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_812600, whole genome shotgun sequence.
ACCESSION   DASVQZ010000195 DASVQZ010000000
VERSION     DASVQZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812600"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..650
                     /locus_tag="VF547_05385"
     CDS             462..650
                     /locus_tag="VF547_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262289.1"
                     /translation="MSRPPKSTPHSDIDGVHRDEKPNVESAVEAGEGAQDLARASEET
                     VARPDYAGDLDNRDDRSK"
     gene            complement(724..>1937)
                     /locus_tag="VF547_05390"
     CDS             complement(724..>1937)
                     /locus_tag="VF547_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX8262290.1"
                     /translation="SAGFAPTAGPARAPKAGTIARIHDDGSALSLFVFGESGTLEGVA
                     EFDRSALRNLTFIPGTRPDFDLDLSQDDRRMVLRSDFKGGAWRQRVEGVRPVSGNQLR
                     LHILLSAVPITLPDMLLILLPVLMWLFAALIGWIILNRLLLNPLGRMQRAVAAYRPGD
                     RQLNLPRVATPAREIADLGEAFEQAVRTLAHHEAELEAAVERQTKLVREVHHRVKNNL
                     QVVASLLNIHSRGSPNEEVAAAYASIQRRVDALAVVHRNHYAELEENRGVALKPLVSE
                     LAANLRATAPASALGMSIRLGLEPYYSTQDVAVSVAFLITEIAEYAMLCGAASVSIAL
                     TGDGAPPGTAQLTIEADSLRAGAGCDSTLTERFERIVTGLCRQLRSTLDRDVDSGRYT
                     LSIAVVDKAER"
BASE COUNT          317 a          621 c          722 g          277 t
ORIGIN      
        1 tgccgtcggc gcagtcggtg gtcgattcga cgtcttccgc ggcgccgtgg accgtggtgc
       61 aggcgctgag cgtcaggccg gtggcgatga gggcgagtgt gaaaagctta cgcatgttcc
      121 gtctcctgga cttcggcggc cggccggatg cgatcggcga ctcgaggtaa ggggagaatg
      181 cggggcggga acgatggttc cggggaagcg acgaagcgcc gggccgccgc gatcggacaa
      241 aatttgagcc cggaggcgcc accggggggg gggaggttgg cgcgtccggg ctcatgtcgt
      301 ggatagcgag agcggggggg caaaaggtca ctatccgaac tgcataacga ggaaggggcg
      361 ggccggttcc gccgttccgc aaaaaatttg catggcggcg aaattttttt tcgaaacctc
      421 ccggccccgc cgccggttct ccgggcgaag aggagcctga cgtgagccga ccgcccaaat
      481 cgacgcccca cagcgacatc gacggagtcc atcgcgacga gaagccgaac gtcgagagcg
      541 cggtcgaggc cggggagggc gcgcaggacc tcgcccgagc gagcgaggag accgtcgccc
      601 ggccggatta tgccggtgac ctggacaacc gggatgaccg gagcaagtga cccgccgttc
      661 ccgcaaaagc ggtgactcgt cgcgccgagc gcggcttgtc tcccttaggc gcccggtcgg
      721 cgatcaccgc tcggccttgt ccaccaccgc gatcgacagg gtgtaacggc cgctgtcgac
      781 gtcccggtcg agcgtcgagc ggagctgccg gcagaggccg gtgacgatgc gctcgaaccg
      841 ctcggtcagg gtggaatcgc accccgcgcc ggcgcgcagg ctgtccgcct cgatggtgag
      901 ctgggcggtt cccggcggcg cgccgtcgcc ggtgagcgcg atgctgaccg acgccgcccc
      961 gcacagcatc gcatattcgg cgatctcggt gatcaggaag gcgaccgaga cggcgacgtc
     1021 ctgagtcgaa taataaggct cgagcccgag ccggatgctc atcccgagcg ccgaggccgg
     1081 agcggtcgcc cgaagattgg cggcgagctc cgagaccagc ggcttgaggg cgacgccgcg
     1141 gttttcctcg agctcggcat aatggttgcg gtggacgacg gcgagcgcgt cgacccggcg
     1201 ctggatcgag gcgtaggcgg cggcgacctc ctcgttgggc gagccgcgcg aatggatgtt
     1261 gagcaaggac gccacgacct ggaggttgtt cttcacccga tggtggacct cgcggaccag
     1321 cttggtctgg cgctcgaccg ccgcctccag ctcggcctcg tgatgggcaa gggtgcgcac
     1381 ggcctgctcg aacgcctcgc ccagatccgc gatctcgcgg gccggcgtcg ccacgcgcgg
     1441 caggttcagc tgcctgtcgc cggggcgata ggcggcgacg gcgcgctgca tccggccgag
     1501 cgggttgagc aggagccggt tgaggatgat ccagccgatc agggccgcga acagccacat
     1561 cagcaccggc agcaggatga gcagcatgtc gggcagcgtg atcggcacgg ccgaaagcag
     1621 gatgtgcagc ctgagctggt tgccggagac cggccgcacg ccctccaccc gctgccgcca
     1681 cgccccgccc ttgaaatccg agcgcaggac catccgccgg tcgtcctggc tgaggtcgag
     1741 atcgaaatcg ggccgggtgc cggggatgaa ggtcaggttg cggagcgcgg agcggtcgaa
     1801 ttccgcgacc ccctcgagcg tcccgctttc gccgaagacg aacaggctga gcgccgagcc
     1861 gtcatcgtgg attcgggcga tcgttccggc cttgggcgcc ctcgccggcc cggcggtcgg
     1921 cgcgaagccc gcgctcg
//
LOCUS       DASVQZ010000196         7225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_522891, whole genome shotgun sequence.
ACCESSION   DASVQZ010000196 DASVQZ010000000
VERSION     DASVQZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522891"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VF547_05395"
     CDS             complement(<1..396)
                     /locus_tag="VF547_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8262291.1"
                     /translation="MATHAHPPLGDQIRAWRGRRRLSQLELALDAEISARPLSFIETG
                     RSRPSRAMLLRLAERLRLPPRERNALLVAGGFAPASPERTLDDPALAAARQAVDKVLA
                     AYEPWPALAVDLHWNLVAANAGVAPFLADV"
     gene            505..894
                     /locus_tag="VF547_05400"
     CDS             505..894
                     /locus_tag="VF547_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262292.1"
                     /translation="MASTTFLRRILALDSASCALLGALLVLDGAALSPLFGLGERLLF
                     GAGLLLLPLAVFIGWLASRTAPPSLLVWLVIVGNLAWTAESLLLIATESGRITAPGTA
                     FVAAQAAAVLVVALLEYAGLRRMRAAA"
     gene            complement(932..1258)
                     /gene="grxD"
                     /locus_tag="VF547_05405"
     CDS             complement(932..1258)
                     /gene="grxD"
                     /locus_tag="VF547_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX8262293.1"
                     /translation="MSDTKSRIDEIVGKNDVVLFMKGTALFPQCGFSSRAVAILDRLG
                     VGFETVDVLQDPEIRQGIKDYSDWPTVPQLYVKGEFVGGSDIMMEMYQAGELQQLLDE
                     KQVAKA"
     gene            complement(1270..1503)
                     /locus_tag="VF547_05410"
     CDS             complement(1270..1503)
                     /locus_tag="VF547_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX8262294.1"
                     /translation="MAMAAGEIEALIRAAIPDARVEITDLAGDGDHYAARVVAESFRG
                     MPRVRQHQRVYEALGGRMGGLLHALQLSTATPD"
     gene            complement(1503..1829)
                     /locus_tag="VF547_05415"
     CDS             complement(1503..1829)
                     /locus_tag="VF547_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX8262295.1"
                     /translation="MTTFDDRERAFETKYARDQDMQFRIVARRNKLLGQWAANLMGLS
                     QAESDAYASDVVRADFEEAGDEDVIRKVLGDLTSAGVECDEGRIREALAHKTVEARRQ
                     LMESQG"
     gene            complement(2028..3026)
                     /locus_tag="VF547_05420"
     CDS             complement(2028..3026)
                     /locus_tag="VF547_05420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8262296.1"
                     /translation="MRALLSNSPGGPETLELTELADPSPGPGQILVRVRACAINYPDV
                     LIIEDKYQFKPPRPFAPGGEIAGTVEAVGEGVEGWAEGDRAIAMIGHGGLQEKVLISA
                     AQAVPLPPERSFEEGSALILTYATSIHALLDRGRLQAGETLLVLGAAGGVGLAAVELA
                     KAYGARVVAAVSSEEKAEAARAAGADEVLIYPQGPFDKEQSKALAHQFKEAVGGKGAD
                     LIYDPVGGDYAEPALRSIAWEGRYLVVGFPAGIPKLPLNLALLKSCDVRGVFWGAFAA
                     RDPKANAAHVETLFRLWREGKIAPRVSRTWPLDQGGEAIAHMAARKAVGKLVVTMG"
     gene            complement(3023..3439)
                     /locus_tag="VF547_05425"
     CDS             complement(3023..3439)
                     /locus_tag="VF547_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8262297.1"
                     /translation="MPFVLDASLALSWHFEDENPDLSVPERAFREGVAVPQHFFLEIA
                     NGLLRGERRLRTAPEGTDTFLARLADLKATIDPTAPELVVSVLVPLSRDHRLSIYDAA
                     YLELAGRLGLDLATCDSSLAEAARRVGIALIEGTGN"
     gene            complement(3445..3708)
                     /locus_tag="VF547_05430"
     CDS             complement(3445..3708)
                     /locus_tag="VF547_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX8262298.1"
                     /translation="MDRPTTEFGVADAKARFSELLARAEAGETIVISRHGRPVAKMVS
                     AGKRELTPDERVKSWQAWVAYRRKHNITLGPDLTIKQLIEEGR"
     gene            complement(3762..4601)
                     /locus_tag="VF547_05435"
     CDS             complement(3762..4601)
                     /locus_tag="VF547_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX8262299.1"
                     /translation="MIKKFTLLAAAWLGLGALQAPPAPRPTIELWRLHCGEFVIKQYG
                     AFFSDTFQYPAGPKDIVGSCYLIRHGDRYMLWDTGLTDALIGHDFDNPAQTIRLKRSL
                     VDQLAELKLKPEQVETIGISHWHFDHTGQAGRYPQAKLLMGKADLDMLRTTPPADEDS
                     AKGLAHWLGGGGKLEEVSADKDVFGDGRVVMLKLPGHTPGHYALLVRLASGPVLLSGD
                     QYHFTEQVKNRGVPPFNHDRADTLASSDRFDRIAANLKAKVIIQHEPADIAKLPAFPK
                     SAR"
     gene            complement(4598..5566)
                     /locus_tag="VF547_05440"
                     /pseudo
     CDS             complement(4598..5566)
                     /locus_tag="VF547_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(5589..7061)
                     /locus_tag="VF547_05445"
     CDS             complement(5589..7061)
                     /locus_tag="VF547_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262300.1"
                     /translation="MPSRPGCLLALLLPLLIAATPPERDWGATLRQDAQALHDDLAAN
                     HPGPVNPEDPDFAPRNRAQLRLALERAATARSFAAYFSAMRAYVASFDDGHLDFGVYG
                     NTPPTQWPGFLTHYEADGEQRVAARAEDAPVPLGARLVGCDGRPAEELAEANVGRFVG
                     RWTLLSQRLAYGNGLFADDGNPYLRRPTRCTFEAAGRKQDVTLRWRPINLGELIGKMR
                     EMRAHPAREIAARRLEDGTSWIALGSFEGDSKSPSGQALASVIAALRADREALAAAPA
                     IVLDLRANTGGSSDWPRQIAEILWGRSALSRLPPDTARVDWRVSEANLASLRESRERQ
                     IAGGALSPAMRRWFDRVTGGIEGALARGETLWREPEDEGEPAAEAAPKTARPRLKGPV
                     YFVTDAGCASACLDAVDLWRALGAVHVGQTTSADTFYMDIRMRRLPSRLGAIVVPMKV
                     YRGRKRGSNQPVAPVHAYAGDISATPALERWIAALPERRR"
BASE COUNT         1048 a         2469 c         2558 g         1150 t
ORIGIN      
        1 gacgtccgcc aggaatggcg cgaccccggc attggcggcg acgaggttcc agtggaggtc
       61 gaccgcgagc gcgggccagg gctcgtaggc cgcgagcacc ttgtcgaccg cctgccgggc
      121 cgcggcgagg gcgggatcgt ccagcgtgcg ctcgggcgaa gcgggcgcga agccgccggc
      181 gacgaggagc gcgttgcgct cgcgcggcgg cagccgcagc cgctccgcga gcctcagcag
      241 catcgcgcgg ctcggccgcg acctgccggt ctcgatgaag ctcaggggcc gcgccgagat
      301 ctccgcgtcg agcgcgagtt cgagctggct aaggcgccgc cgcccgcgcc acgcgcggat
      361 ctggtcgccg aggggcgggt gggcgtgggt ggccatggcc cagcctaacg cggcgggccg
      421 gggaatccca ttacctgcga ggtaatcgac agcatgacct tgcgcggcgg atgaagggcc
      481 gtcgttcaga tgaaaggatc gatcatggct tcgaccacat tcctccgccg aatcctggcc
      541 ctcgactcgg cgagctgcgc tctgctcgga gcgctcctcg tcctcgacgg cgccgccctc
      601 tcgcctttgt tcgggctcgg cgagcggctg ctgttcggcg ccggcctcct gctcctgccg
      661 ctcgccgtct tcatcggctg gctggcgtca cggacggctc cgcccagcct cctcgtgtgg
      721 ctcgtcatcg tcggcaacct cgcctggacc gccgagagct tgctgctgat cgcgacggag
      781 agcgggcgta tcaccgcgcc gggaaccgcc ttcgtcgccg cccaggcggc ggcggtgctc
      841 gtcgtggcgc tgctcgaata tgccggcctt cgccgcatgc gggccgcggc gtgagaaatt
      901 cctccccgcg atttcgcggg gaggaattta gtcaggcctt cgccacctgc ttctcgtcga
      961 gcagctgctg gagctcgccg gcctggtaca tctccatcat gatgtcggag ccgccgacga
     1021 actcgccctt gacgtagagc tgggggacgg tgggccagtc ggaatagtcc ttgatgccct
     1081 ggcggatctc gggatcctgc agcacgtcga ccgtctcgaa gcccacgccc aggcggtcga
     1141 gtatggcgac cgcccggctc gagaagccgc attgcgggaa gagggcggtg cccttcatga
     1201 acaggacgac gtcgttcttg ccgacgatct cgtcgattcg ggacttggtg tcggacattt
     1261 cggatttcct cagtcgggtg tcgcggttga gagctggagg gcgtgcaaga gcccgcccat
     1321 gcggccgccg agcgcctcgt agacgcgctg gtgctggcgc acgcggggca tccctcggaa
     1381 gctttcggcg accacccgcg cggcataatg gtcgccgtcg ccggcgagat cggtgatctc
     1441 gacccgggcg tcggggattg cggcccggat cagcgcctcg atctcgccgg ccgccatcgc
     1501 catcagccct gcgactccat cagctggcgg cgggcctcga cggtcttgtg ggcgagcgcc
     1561 tcgcggatcc gcccctcgtc gcactcgacg cccgccgaag tcaggtcgcc gagcaccttg
     1621 cggatcacgt cctcgtcgcc ggcctcctcg aaatcggcgc ggacgacgtc gctggcatag
     1681 gcgtcggact cggcctggct gaggcccatc aggttcgccg cccattggcc gagcagcttg
     1741 ttgcgccgcg cgacgatccg gaactgcatg tcctggtcgc gggcatattt ggtctcgaag
     1801 gcccgttcgc ggtcgtcgaa ggtggtcatg gcggtccttt gctgtgctgg agagtcagat
     1861 aggcccggcg cgtgggaggt gcaacggcgg gcttacactc ctccccggaa cggggagggg
     1921 gaccatgcga agcatggtgg aggggtcccg agtctccgcc gttcttgcgc tggccccctc
     1981 caccaccgcc ttcggcgggg gtccccctcc ccgttccggg gaggaactca ccccatcgtc
     2041 accacgagct tcccgaccgc cttccgcgcc gccatgtggg cgatcgcctc gccgccctgg
     2101 tcgagcggcc aggtccggct gaccctggga gcgatcttgc cttcgcgcca cagccggaac
     2161 agggtctcga catgggccgc gttggccttc gggtcgcgcg ccgcgaaggc gccccagaag
     2221 actccgcgca cgtcgcagct cttgagcagg gcgaggttca ggggcagctt cgggattccc
     2281 gccgggaagc cgacgacgag atagcgcccc tcccacgcga tcgagcgcag ggcgggctcg
     2341 gcatagtcgc cgccgaccgg atcgtagatc aggtccgcgc ccttgccgcc cacggcctcc
     2401 ttgaactgat gggcgagcgc cttcgactgc tccttgtcga acggcccctg cggatagatc
     2461 agcacctcgt cggcgcccgc ggcccgcgcg gcttcggcct tctcctccga cgacaccgcc
     2521 gcgaccaccc gggcgccata ggccttggcg agctcgaccg ccgccagacc gacgccgccg
     2581 gccgcgccta ggacgagcag ggtctccccg gcctgcagcc ggccgcggtc gagcagggcg
     2641 tggatcgagg tcgcataggt caggatcagc gccgacccct cctcgaagct tcgctcgggc
     2701 ggaagcggca ccgcctgcgc ggcggaaatc agcaccttct cctgaagccc gccatggccg
     2761 atcatcgcga tcgcccggtc gccctccgcc cagccttcga ccccctcccc gaccgcctcg
     2821 acggtcccgg cgatctcgcc ccccggagcg aagggccgcg gcggcttgaa ctgatatttg
     2881 tcctcgatga tcagcacgtc gggatagttg atcgcgcagg cgcggacccg gacgaggatc
     2941 tggcccgggc cgggcgaagg gtcggcaagc tcggtcagct ccagggtctc tggtccgccg
     3001 ggtgaatttg acagtaacgc cctcaattgc ccgttccttc gataagagcg attcccactc
     3061 gccgagccgc ctcggccagc gaagagtcgc acgtcgccaa atccaagcca agccgtccgg
     3121 caagctccag gtaggcggcg tcatagatcg acagtcgatg gtcgcgcgag agcggtacca
     3181 ggacggagac gacaagctcg ggtgcggtcg ggtcgattgt cgccttcagg tccgccagcc
     3241 gggccaggaa ggtatcggtg ccttcgggcg cggtccgaag gcgtcgctcg ccgcgcagga
     3301 ggccgttcgc gatctccaga aaaaagtgct ggggcaccgc cacgccttcg cgaaaggccc
     3361 gctcggggac ggacagatcc ggattttcgt cttcaaaatg ccatgagagt gccagggatg
     3421 catccaggac gaagggcatc agtatcagcg gccttcctcg atcaattgct tgatcgtgag
     3481 gtcgggacca agagtgatgt tatgcttgcg gcggtaggcg acccacgctt gccacgattt
     3541 cactcgctcg tctggcgtca gttcccgttt ccctgccgac accatcttcg cgacgggacg
     3601 cccgtgacgg ctgatgacga tagtttcgcc ggcttccgcc ctggcgagca gctctgaaaa
     3661 gcgcgccttg gcgtcggcga ccccgaattc cgtggtaggg cggtccatat tttgcccttt
     3721 cagctggcca taatctggcc ctgaacaggt cagccgtcaa gtcaccgcgc tgatttcggg
     3781 aaggccggga gcttggcgat gtcggccggc tcgtgctgga tgatcacctt cgccttgagg
     3841 ttggcggcga tgcggtcgaa ccggtccgac gaagcaagcg tgtctgcgcg gtcgtggttg
     3901 aacggcggca ccccgcggtt cttcacctgc tcggtgaaat gatattggtc gccggagagg
     3961 aggaccgggc ccgaggcgag gcggacgagc aaggcgtaat ggcccggcgt gtggccgggc
     4021 agcttcagca tgacgacccg gccgtcgccg aacacgtcct tgtcggcgct gacctcctcc
     4081 agtttgccgc cgcctcctag ccagtgagcg aggcccttgg ccgaatcctc gtcggccgga
     4141 ggggtcgtcc ggagcatgtc gaggtcggcc ttgcccatca gcagcttcgc ctgcgggtat
     4201 cggccggcct gtccggtatg gtcgaaatgc cagtggctga tcccgatcgt ctcgacctgc
     4261 tcgggcttga gcttcagctc ggcgagctgg tcgaccaggc tgcgcttgag gcggatggtc
     4321 tgcgccggat tgtcgaaatc gtggccgatc agggcgtcgg tgaggccggt gtcccacagc
     4381 atgtagcggt cgccgtggcg gatcaggtag cagctgccga cgatgtcctt ggggccggcc
     4441 ggatattgga aggtgtcgga gaagaaggcg ccgtactgct tgatcacgaa ctcgccgcaa
     4501 tggagtcgcc acagctcgat cgtgggccgc ggcgccggcg gcgcctgcag ggcgccaagg
     4561 ccgagccagg cggcggcgag cagcgtgaat ttcttgatca tccgagggcc ccttctttcg
     4621 gcgagacata attgggatcg tggacgaagg gcaatggccg cccgtcccgc agcgcggcca
     4681 ggatcgcggc gaagtcggtg cgggcccgga agccgagctc ccgctccgcc agcgaggggt
     4741 cgtagacccg gtcgagatgc gccggcagcc gccagcccga gcgcgcgtag agctgcgccg
     4801 cctccgggaa atggcgggcg acgacggcgg gcgcgtcctc gatcagcgcc tcggcttcgt
     4861 cctgagtgaa cggcggcggg gcggagacga tgtagaggcc gaagccgatc tccggcgcct
     4921 tggcgagcgc ggcgacatgc gcctcggcgc aatcctcgac ggtgagacgc cggttgagga
     4981 actcgttggc cttggtgttg gggccggaaa gctcgcggtg ggtgtcgtcc tcctcgggga
     5041 agaagcggcc ggtgcgcaga acgacgaccg gcagcccgtg ctcccggtgg acgagggcgc
     5101 acagctgctc ggcggcgcgc ttggtcacgc cgtagacgtt gcggggctcg agcggcccat
     5161 attcctcggt cagccaggcg gcccgcttcg ccccgcccgc cttgccggcc cggatcgcct
     5221 gcgagatcat cagcgaggtc gtcgaggtga agacgaagcg gtctcagccc gctgcggcgg
     5281 cggcctcgag caggttcagc gtgccggtga cgttgacgtc gacgaaggcc tgcttcgggt
     5341 agcggacgat gtcgggcttg tgcagcgctc cggcgtggac gacggcgtcg atgccgtgat
     5401 cgccgatcgc gcgctcgacg agcccgcgct cggcgaccga gccgaccacc cgcgtgtcgg
     5461 gtcccgccgc gacgtcgagg ccggtcacct cgtgcccgtc gctgcgcagg cgcggcgcca
     5521 ggaaccggcc gagccagccg gacgagccgg tcaggaggat gcgcatatcg tctcgggtct
     5581 ccgaaggttc agcgccggcg ctccggaagc gccgcgatcc agcgctccag cgcgggcgtc
     5641 gcggagatgt cgccggcata ggcgtggacc ggagcgacgg gctggttgga gccgcgtttg
     5701 cggccgcgat agaccttcat cgggacgacg atcgcgccca gcctgctcgg cagccggcgc
     5761 atccggatat ccatgtagaa ggtgtcggcg ctggtcgtct ggccgacatg gaccgcaccc
     5821 agagcgcgcc acaagtccac cgcgtcgagg caggcggacg cgcacccggc atcggttacg
     5881 aaatagacgg ggcccttaag cctgggccgg gcggtcttcg gtgccgcttc cgccgccggt
     5941 tccccttcgt cctccggctc ccgccatagg gtttcgccgc gcgccagcgc gccttcgatc
     6001 ccgccggtca ctcggtcgaa ccatcggcgc atcgccggcg aaagcgctcc gcccgcgatc
     6061 tggcgctcgc ggctctcgcg cagcgaggcg aggttggcct cggagacccg ccagtcgacc
     6121 cgggccgtgt ccggcggaag ccggctcagc gcggaacgcc cccagaggat ctcggcgatc
     6181 tgccggggcc agtcggacga gccgccggtg ttggcgcgca ggtcgagcac gatcgcgggc
     6241 gcggccgcca gggcctctcg atcggcgcgc agagcggcga tgaccgatgc cagcgcctgg
     6301 ccggatggcg atttcgaatc gccctcgaac gagccgagcg cgatccagct tgtgccgtcc
     6361 tcgagcctcc gggcggcgat ctcgcgcgcg ggatgcgccc tcatttccct catcttgccg
     6421 atcagctcgc caaggttgat cggccgccag cgcagggtaa cgtcctgctt gcggcccgcc
     6481 gcctcgaacg tgcaccgcgt ggggcgccgg aggtacggat tgccgtcgtc cgcgaacagg
     6541 ccgttgccat aggccagccg ctgcgagagc aaggtccagc gcccgacgaa ccggccgaca
     6601 ttggcttccg ccagctcttc ggcgggccgg ccgtcgcagc cgacgagccg ggcgccgagc
     6661 ggcacgggcg cgtcctcggc ccgggccgcc acgcgctgct cgccgtccgc ctcgtaatgg
     6721 gtgaggaatc caggccattg ggtgggcgga gtattgccgt acaccccgaa atcgaggtgg
     6781 ccgtcgtcga acgaggcgac ataggcccgc atcgcggaga aataggcggc gaagcttcgg
     6841 gcggtcgcgg cgcgctcgag cgccagccgg agctgggccc ggttgcgcgg cgcgaaatcc
     6901 ggatcctccg gattgaccgg gcccgggtgg ttggcggcga gatcatcgtg gagcgcctga
     6961 gcgtcctggc gcagcgtcgc gccccagtcc cgctcgggcg gagtcgccgc gatcagcagc
     7021 ggaaggagca gggctaggag gcaaccgggc cggctcggca tgcccgaacc ctatggcccg
     7081 tccggaagcc ggtcaatcgc aggactaggc tgtatcgaca ttcgccggca ctgctccaat
     7141 ccccctctcc cccgggggga gagggcgggg gtgagggggc tcggcggtcc gagagcgatc
     7201 accatgcgcc gagccccctc tccct
//
LOCUS       DASVQZ010000197         7297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_523054, whole genome shotgun sequence.
ACCESSION   DASVQZ010000197 DASVQZ010000000
VERSION     DASVQZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523054"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..314)
                     /locus_tag="VF547_05450"
     CDS             complement(177..314)
                     /locus_tag="VF547_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262301.1"
                     /translation="MTDDKKKGDFNKGGKDDVGGDKKQRADRNDDSRTGNQNSGSGGR
                     D"
     gene            569..1033
                     /locus_tag="VF547_05455"
     CDS             569..1033
                     /locus_tag="VF547_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262302.1"
                     /translation="MPQDSKSKQARDIHPLLRSSQTARHIVRLSAKSTSEYDLYDEYM
                     STVGRALFIPNPKRDVAENLDALREAGLVEGREGGTVQASRDAVRQVQATAFDDVTVE
                     LIESPGFRAIMAPGVEQFRFHLASCSTSTSCKCSSSSCSSSKGKAAELASAS"
     gene            1084..2172
                     /locus_tag="VF547_05460"
     CDS             1084..2172
                     /locus_tag="VF547_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HEX8262303.1"
                     /translation="MPRAGAGTAAARRAGPVRELPDRRPAMTAGLPRRPALNGACNIV
                     PVDGGSALAVTASSTFALSGEGFGELFPFLLFLLDGRHSVAGIVERLETFCEADVLRA
                     LELLNRNGLLEEGDGDPAFDGPEGQARFFSGYGLGRASDAQSKLRTAQVGLLGTRSAA
                     AKLDGELAECGVPPLRKPADGAMPDETQLAAFLEGLSLLIVVTSDWPVEFCERINRAA
                     LATGVAWLACSVEARGASVGPLFLPGETACFHCFSRRRRGAEDFIAAYDAMSRAWTGE
                     FHDRLGLCPGLSGLVSGLAVVETVKFLTGFEVPTAVGAEWRFELRSGQLERHRVLKLP
                     RCPECGPAHLHLPQASIWSGFMDEESRR"
     gene            2169..3506
                     /locus_tag="VF547_05465"
     CDS             2169..3506
                     /locus_tag="VF547_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX8262304.1"
                     /translation="MSGASGDRACLARARPAISARSGIIKSFHELLIEPDDASLFNCF
                     TQVAATSRYAADTEDCYQQNGGAALTRDGARAAAIGETLERYSCSVYDRSQMLLATWR
                     ELRDRNLRALHPRDAPLYADDQYDLPGFVPERFTETTMLRWVPAWSLTKACETWIPAV
                     FAYIPYYCEPPESLILYSVSTGLSCALDRESAVLSGLYESVERDAVMISWLNRLPRRR
                     LDPASDAEVARVFAERFAGRGTEYMIFDFSTDLGVPVIFAAAVDRNHDQLALACGAAA
                     NFDVRRAAIKALTEAAQGRIWLKHMRQTGESYERPASFSEIRSFEDHVRLYGCREMLA
                     KADFLIDQQELHPIPGCAERPPGPKAAIRRLVERLDQAGLEALVLDLTPPDVRSLGFH
                     VVKTVVPGLQQLFADHNYRMLGAPRLYEVPARLGYRQGPTDPSEINPDPHPFP"
     gene            3519..4370
                     /locus_tag="VF547_05470"
     CDS             3519..4370
                     /locus_tag="VF547_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX8262305.1"
                     /translation="MQFLFNTDDIVPPEARRLGELFHENSKLHPRASAELGRWIGELM
                     ENPAALSTMSQSFKRYDNAPSVPLPAADPPDRPLPEILARRRSVLSGSSEPKFSGRPL
                     GGRDLSILLGQACGITEVREMEAHGVKFLQRLRAYPSGGGLQPIEIYPILQNVDDAYA
                     GVHHYDVVNHRLEKLGDRLAPAQLDALCSFGDLLREAAAIIVLTAVFSRSYFKYHDRG
                     YRFVLLEAGHVAQNLYLQATALDLAVVAVGGFFDDAVNRLLGIDGLNEAAVYLVVVGH
                     PPAPAAS"
     gene            complement(4395..5186)
                     /locus_tag="VF547_05475"
     CDS             complement(4395..5186)
                     /locus_tag="VF547_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat biosynthesis protein F"
                     /protein_id="HEX8262306.1"
                     /translation="MTMPRILVLAGRRSTTPDPLAVKMGVSHKALVPVAGEAMIGRVL
                     RIAAEAFPESPLFVSVEDFAIVADEPTVARLHAEGRLTPVGAEDHIVDSVVEASRTTG
                     FPLLLTTADNVLMSVDGFRAIAEAGSAPGVDGVEMMAEKADILAAHPDGQRRFYAFRG
                     GAYSNCNLFWITGEHSFRGIHSFRYGGQFAKRKMDAVRALGLGSLLLYLTGLVTLEGM
                     FRHFSRRFGIRIVPLVAKDGRFAIDVDNERTHRVAEEILARGRGD"
     gene            complement(5204..6679)
                     /gene="rpoN"
                     /locus_tag="VF547_05480"
     CDS             complement(5204..6679)
                     /gene="rpoN"
                     /locus_tag="VF547_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX8262307.1"
                     /translation="MSIGPRLDLRQSQSLVMTPQLQQAIRLLALSNVEVESFIAEEIE
                     RNPLLDTGGGDEGAPAEPAPEAAEEAPAADAFGSGEAPLDLDVNDENLHQDSAADRAL
                     DGGLGMEGLSGGGWDGEGPDFDSFAAEGQSLHAYLLAQAGERLSGPDLVIAGQIIEQI
                     DETGYLLASTLDLAGRLGVPLAEVERVLAVVQSFDPAGVAARSLAECLALQAKEADRY
                     DPAMARLIDNLDYLAKGNFAALKRICGVDDEDLGDMVRELRAYDPKPGCRFAGSERVE
                     AVVPDVFVARRGFGWAVELNSATLPRLLVNRRYYAELTGGADRRSKAWLSECLASANW
                     LLKALDQRARTIVKVATEIVKQQDGFFRHGVAQLKPLTLRQVAEAIGMHESTVSRVTS
                     NKYLSCDRGLYELKYFFTSGIQSSDGGDAVSAEAVKSHIKALIAAEDPRAILSDDQLV
                     ELLRAKGFDIARRTVAKYREAIGLGSSVQRRRQKAIGSHAA"
     gene            complement(6795..>7297)
                     /gene="lptB"
                     /locus_tag="VF547_05485"
     CDS             complement(6795..>7297)
                     /gene="lptB"
                     /locus_tag="VF547_05485"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX8262308.1"
                     /translation="RAILGLGYLPQETSIFRGLSVEENIMAVLQVAEPDRHARRERLE
                     QLLGEFGLTRLRESSAMALSGGERRRCEIARALAADPSIMLLDEPFAGIDPISIGDIR
                     DLVIELKKRDIGVLITDHNVRETLEIVDRACIIYDGRVLFAGSPAELVADPDVRRLYL
                     GESFEL"
BASE COUNT         1136 a         2490 c         2480 g         1191 t
ORIGIN      
        1 cggagccctt cttcatcctc cccatcgccg atggggaggg ggaccgcctg caaggcggtg
       61 gaggggtaaa tggcgcggaa gcagagggac ccaccggaac ctcccgggtc cgagccagct
      121 acccctccac catgcttcgc atggtccccc tccccatttg gcgatgggga ggacgatcag
      181 tcccggccgc ctgaaccgct gttctgattg ccggtgcggc tgtcgtcgtt gcggtcggca
      241 cgctgcttct tgtcgccgcc gacatcgtcc ttgccgccct tgttgaaatc gcccttcttc
      301 ttgtcgtccg tcatgagcct ctccttcggc agcgagaacg gccggcccgg gctggagttt
      361 cgaacgcggg ggcgatcgcc agcgggcctc gaacttgcgc cataatggtc caatctgtga
      421 ctggcagatt cgcgccgcgg gtctaagctc ggcgggcgaa agagcgtcct gcgccagggc
      481 cggcacgccg ccggccgcga gagcgccaac gctcgatcaa gcgtgtttgt aggctgctca
      541 tccgtcatcc aaggcgatgg aggttttcgt gccacaggac tccaaatcga aacaagcgcg
      601 cgacattcac ccgctgctcc ggtcgagcca gaccgcgcgc cacatcgtgc ggctcagcgc
      661 caagtcgacg agcgaatacg acctctacga cgaatatatg agcaccgtcg gccgagcgct
      721 gttcattccc aatcccaagc gggacgtcgc cgagaatctc gacgccctgc gcgaggccgg
      781 cctcgtcgag ggccgtgagg gcggcaccgt gcaggcatcg cgggacgccg tgcggcaggt
      841 ccaggccacc gctttcgacg acgtgaccgt cgagctcatc gaaagccccg gcttccgggc
      901 catcatggcg ccgggcgtcg agcagttccg gttccatctg gcgtcgtgca gcacctcgac
      961 cagctgcaaa tgctcgtcat cctcatgcag ctcgtcaaaa ggcaaggccg ccgaactggc
     1021 ctccgcgtcc tagccgcgga tcgaggctga catgccgggt cgacgtctcc cgcgccgcaa
     1081 aggatgccgc gcgccggcgc cggcacggcg gccgcgaggc gggccggccc ggtccgagag
     1141 ctgcccgacc gccggccggc gatgacggcg gggctgcccc gacgccccgc gctcaacggc
     1201 gcctgcaaca tcgtcccggt cgacggcgga agcgcgctcg ccgtcaccgc ttccagcacc
     1261 tttgccctgt cgggggaggg gtttggggag cttttcccct ttctcctgtt cctgctcgac
     1321 ggacggcaca gcgtcgccgg aatcgtggag cggctagaga ccttctgcga ggcggacgtg
     1381 ctgcgcgcgc tcgagctgct caaccggaac gggctgctcg aggaggggga cggcgatccg
     1441 gccttcgacg gccccgaagg gcaggcccga ttcttctccg gctacggact gggccgagct
     1501 tcggatgccc agtcgaagct gcggacggcg caggtcggac tgctcggaac gcggtcggcc
     1561 gcggcaaagc ttgacggcga gctcgccgag tgcggcgtgc caccgcttcg caagcccgcc
     1621 gacggcgcca tgcccgacga gacgcagctc gcggccttcc tggaaggcct gtccctcctg
     1681 atcgtggtca ccagcgactg gccggtggaa ttctgcgaaa ggatcaaccg ggcggcgctg
     1741 gcaacgggcg tcgcctggct ggcctgctcg gtcgaagctc gcggggcgtc ggtaggtccc
     1801 ctcttcctgc ccggggagac cgcctgcttc cactgcttct cgcggcggcg tcgcggcgcg
     1861 gaggatttca tcgcggccta cgatgccatg tccagggcct ggaccggcga gttccacgac
     1921 cggctcggcc tttgcccagg cctgtcgggc ctggtctccg gactcgcggt cgtcgaaacc
     1981 gtcaagttcc tgaccggatt cgaggtgccg acggcggtcg gtgccgaatg gcgcttcgag
     2041 ctccgctccg ggcagctcga gcgccatcgc gtgctcaagc tgccgcggtg cccggaatgt
     2101 ggaccggcgc atctccatct gccccaggcc tcgatctggt ccggcttcat ggacgaagag
     2161 tcccggcggt gagcggcgca agcggcgatc gggcctgtct ggcaagggcc cggccggcga
     2221 tcagcgcgcg gagcggcatc atcaaatcat tccatgagct gctgatcgag ccggacgacg
     2281 cgagcctgtt caactgcttc acccaggttg ccgccacctc gcgctacgcc gccgacacgg
     2341 aagactgcta tcagcaaaat ggcggcgccg ccctcacccg cgacggcgcc cgcgcggccg
     2401 ccatcggcga gacgctcgag cgttattcgt gcagcgtcta cgatcgcagc cagatgctgc
     2461 tggcgacatg gcgcgagctt cgggaccgaa acctgcgagc gcttcatccc cgcgacgccc
     2521 cgctctatgc cgacgaccaa tatgacctgc ccggcttcgt ccccgagcgc ttcaccgaaa
     2581 ccaccatgct tcggtgggtg ccggcctggt cgctgactaa agcttgcgag acctggatcc
     2641 ctgcggtctt cgcctacata ccctattatt gcgagccgcc cgaaagcctg atcctctatt
     2701 ccgtctcgac cggattgagc tgcgcgctcg atcgcgaaag cgcggtcctc agcggccttt
     2761 acgagtcggt cgagcgggac gccgtgatga tcagctggct gaaccggctc cctcggcgac
     2821 ggctcgaccc ggcctcggat gcggaggtcg cgcgcgtctt cgccgagcgc ttcgccggac
     2881 gcggcaccga atatatgatc ttcgatttca gcacggacct cggggtcccg gtgatcttcg
     2941 ccgccgcggt cgacaggaac catgatcagc tggcgctggc ctgcggcgcc gcggcgaatt
     3001 tcgatgtccg aagggccgcg atcaaggcgc tgaccgaagc ggcgcagggg cggatctggc
     3061 tcaagcacat gcggcagacg ggcgagtcct atgaacgccc ggcgagcttt tcggaaatcc
     3121 gcagtttcga ggaccacgtc cggctctacg gatgccgcga aatgctggcc aaggccgatt
     3181 tcctgatcga ccagcaggag ctccatccga ttcccggctg cgcggaacgt cccccggggc
     3241 ccaaggcggc gatccgccgg ctggtcgagc ggctcgacca ggccgggctc gaagcgctcg
     3301 tcctcgacct caccccgccc gacgtgcgca gtctcgggtt ccacgtcgtg aaaaccgtcg
     3361 tccccggcct ccagcaactc ttcgccgacc acaattaccg gatgctgggc gcgccgcggc
     3421 tctacgaggt gccggcccgg ctcggttatc gccagggacc gacggatccg tccgagatca
     3481 atcccgatcc gcatcccttt ccctaaaagg ggctcgccat gcagtttctg ttcaacaccg
     3541 acgacatcgt ccctcccgag gcgaggcgcc tcggggaatt gttccatgag aacagcaagc
     3601 tccacccccg cgccagcgcg gaactggggc gttggatcgg cgagctgatg gagaatccgg
     3661 cggcgctcag cacgatgagc caatccttca agagatatga caacgccccg agcgttcccc
     3721 tcccggcggc cgacccgccc gaccggccgc tccccgagat actggcgcga cggcggtcgg
     3781 tgctttcggg atcctcggag ccgaaattct ccgggcggcc gctgggcggg agggacctgt
     3841 cgatcctgct cggccaggcc tgcgggatca ccgaggtccg cgagatggag gcgcacggag
     3901 tcaagttcct ccagcggctt cgggcctatc cttcgggcgg cgggctccag ccgatcgaaa
     3961 tctatcccat cctccagaat gtcgacgacg cttatgccgg ggtgcatcat tacgacgtcg
     4021 tcaaccatcg cctggagaag ctcggcgacc ggcttgcgcc cgcccagctg gacgcgcttt
     4081 gctcgttcgg ggacctcctc cgggaagcgg ccgcgatcat cgtcctcacc gcggtcttct
     4141 cgcgaagcta tttcaaatat cacgatcgcg gatatcggtt cgtcctgctc gaagccggtc
     4201 atgtcgccca gaacctgtac cttcaggcca ccgcgctcga cctcgcggtg gtggcggtcg
     4261 gcggcttctt cgacgacgcc gtcaatcggc tgctcggcat cgacggattg aacgaggcgg
     4321 ccgtctatct ggtggtggtc ggacatccgc ctgcgccggc cgcatcgtga aacggtgaac
     4381 gcagtatcgc cgggtcagtc gccgcggccg cgggccagta tctcctccgc cacccggtgc
     4441 gtccgctcgt tgtcgacgtc gatggcgaag cgcccgtcct tcgcgaccag cggcacgatg
     4501 cggattccga agcggcgcga gaagtggcgg aacatgccct cgagcgtcac caggccggtg
     4561 aggtagagga ggagcgagcc gaggccgagc gcccgcaccg cgtccatctt gcgcttggcg
     4621 aactggccgc cgtagcggaa gctgtggatc ccccggaagc tgtgctcgcc ggtgatccag
     4681 aacaggttgc agttggaata ggcgccgccg cgaaaagcgt agaaacggcg ctggccgtcg
     4741 ggatgggcgg cgaggatgtc cgccttctcc gccatcatct cgacgccgtc cacgcccgga
     4801 gccgagcccg cctccgcgat cgcccggaat ccgtctacgc tcatcagcac gttgtcggcc
     4861 gtggtgagga gcagcgggaa gccggtggtg cggctcgctt cgacgacgct gtcgacgata
     4921 tggtcctccg ccccgaccgg agtcagccgc ccttcggcgt gaaggcgcgc gacggttggc
     4981 tcgtcggcga cgatcgcgaa atcctcgacc gagacgaaca ggggcgattc ggggaaggct
     5041 tcggcggcga ttcgaaggac tcgcccgatc atcgcctcgc cggcgaccgg gacgagcgcc
     5101 ttgtggctga cgcccatctt caccgccagc ggatccggcg tggtggaccg gcgtccggcg
     5161 aggacgagga ttcggggcat cgtcatagga agcgggaaat acctcaggcg gcgtgggagc
     5221 cgatcgcctt ctggcggcgg cgctggacgg aactgcccag tccgatcgcc tcccggtact
     5281 tggcgacggt ccgccgcgca atgtcgaacc ctttcgcgcg gagcagctcg acgagctggt
     5341 cgtcggagag gatggcgcgc gggtcctcgg cggcgatcag ggccttgatg tggctcttca
     5401 ccgcctcggc cgagacggcg tcgccgccgt cgctggactg gattcccgag gtgaagaaat
     5461 atttgagctc gtagaggccc cggtcgcagg acaaatattt gttcgaggtg acccggctca
     5521 ccgtcgattc atgcattccg atcgcttcgg ccacctggcg cagggtgagc ggcttcagct
     5581 gcgcgacgcc gtgtcggaag aagccgtcct gctgcttgac gatctcggtg gcgaccttga
     5641 cgatcgtccg ggcgcgctgg tcgagcgcct tcagcagcca gttggcgctg gcgaggcatt
     5701 cgctgagcca ggccttcgag cggcggtcgg cgccgccggt cagctcggca taatagcggc
     5761 ggttgacgag caggcgcggg agggtggcgg agttgagctc caccgcccag ccgaagccgc
     5821 ggcgggcgac gaagacgtcg ggcacgaccg cctcgacccg ctcgctcccg gcgaagcggc
     5881 agccgggctt ggggtcatag gccctgagct cgcggaccat gtcgcccaga tcctcgtcgt
     5941 cgaccccgca gatccgtttc agcgcggcga aattgccctt ggcgagatag tcgagattgt
     6001 cgatcagccg cgccatcgcg gggtcgtagc ggtccgcctc cttggcctgg agggcgaggc
     6061 attcggcaag gctgcgcgcg gcgaccccgg ccgggtcgaa gctctggacc acggcgagca
     6121 cccgctccac ctcggcgagc gggacgccga gccggccggc gagatcgagc gtcgaggcga
     6181 gcagatagcc ggtctcgtcg atctgctcga tgatctggcc ggcgatgacg agatccggcc
     6241 cggacagccg ctcgcccgcc tgggcgagga gataggcgtg gagcgactgg ccctcggccg
     6301 cgaacgaatc gaagtccggc ccctcgccgt cccagccgcc tccggacagc ccttccatgc
     6361 cgagcccgcc gtcgagggcg cggtcggcgg cgctgtcctg gtggagattc tcgtcgttga
     6421 cgtcgaggtc gagcggcgcc tcgcccgagc cgaaggcgtc ggcggcgggg gcttcctccg
     6481 cggcctcggg cgcgggctcg gccggcgcac cctcgtcgcc gccgccggtg tcgagcagcg
     6541 gattgcgctc gatctcctcg gcgatgaagc tctcgacctc gacgttggac agcgccagca
     6601 ggcggatcgc ctgctgaagc tgcggagtca tcaccagcga ctggctctgg cgaaggtcga
     6661 ggcgcgggcc gatgctcatg gccgcctcga cccgtcatcg cggggagcga agcgacgaag
     6721 caatctgctc gtcgggagcg gcgcggactg gattgcttcg cttcgctcgc aatgacgagg
     6781 ggggcgcgcc atcgctaaag ctcgaagctc tcgccgagat agaggcggcg cacgtcgggg
     6841 tcggcgacca gctcggccgg gctgccggcg aacagcaccc tgccgtcgta gatgatgcag
     6901 gcgcggtcga cgatctcgag agtctcgcgg acgttgtgat cggtgatgag gactccgatg
     6961 tcgcgcttct tcagctcgat gacgaggtcg cggatgtcgc cgatcgagat cgggtcgatg
     7021 ccggcgaacg gctcgtcgag gagcatgatc gacgggtctg ccgccagcgc gcgcgcgatc
     7081 tcgcagcgcc tgcgctcgcc ccccgacaag gccatcgcgg aggattcacg cagccgggtg
     7141 aggccgaact cgccgagcag ctgctcgagc cgctcgcggc gggcatggcg gtcaggctcg
     7201 gccacctgga gcacggccat gatgttttct tcgaccgata ggccccgaaa gatcgaggtc
     7261 tcctgcggca aatagccgag gccgaggatc gcgcggc
//
LOCUS       DASVQZ010000198         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_332015, whole genome shotgun sequence.
ACCESSION   DASVQZ010000198 DASVQZ010000000
VERSION     DASVQZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332015"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF547_05490"
     CDS             complement(<1..329)
                     /locus_tag="VF547_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262309.1"
                     /translation="MTARPASPVDEAPPALGDEHLARAARTLRIEPDRWARVLSGYAD
                     RSPGPDSAPEARLRALHAAFAEQMADAAPLADEAPSEACPVCPERSVAPRYARGDGVG
                     GPPLFLYG"
     gene            complement(352..975)
                     /locus_tag="VF547_05495"
     CDS             complement(352..975)
                     /locus_tag="VF547_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HEX8262310.1"
                     /translation="MGVAIAGAQYLWPGRTIPYVIDPQLPDAEAVEAAILHWNEHTVI
                     RFVPRTSETDYVLVTRIDGCAVSDIGRRGGKQTICLAEDCPVGSIIHELGHTVGLWHE
                     HCRHNRDDFVTVLLDLIDQDSLQNFTIDAVGDEPMPTFNIGDYDFGSIMHYSETACAF
                     IEGTSVLIPKQPLPPGVVQGQRNGLSPGDVATVEQLYAGVPGPAGES"
     gene            1229..3031
                     /locus_tag="VF547_05500"
     CDS             1229..3031
                     /locus_tag="VF547_05500"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HEX8262311.1"
                     /translation="MALSAGLLFSVAGCDWFDRDDRPPPPAGWPDGGQGWDRAQRLSW
                     YQAGQGSRLVPWPWAKALEQKDGEQLFLAEANMTRFGFLPLAESEAAPKLPIGFAIDR
                     QPDDKLTFTKLRWYEGQQNKEEWLGLNCSACHTGRMNFEGKTITVDGAPSLVDFQMFI
                     EEFDAALDATYSQDAKFDRFAAKVLAGRDNPANRAKLRGELKKLLDWEKANAKLNATS
                     MRYGFGRLDAVGHIFNKVAQLAVYGTNLPAKPNPADAPVSYPFLWDIYRQSQLQWDGI
                     VKTQRLNLGAGYLDYGALGRNVGEVVGVFGDVDLRPASRLAKTGFNSSIQAENLNNLE
                     TVLRKLEAPKWPGALDRAKVDQGKALYSSKGCIGCHTIEPAGDNIYQVHMTPLTGSPT
                     TPNRNNTDPWMACNAITYASFSANLKDLPSGYFKGDPLPAQAQLSDMLTTTVIGTLVA
                     KWNQVLNSAVQVFFGRVPLPKVTRASDQQTDAMKRAARLAQCYESKSPLFAYKARPLD
                     GVWATAPFLHNGSVPNLYELLRAPADRVKTFNVGTRQYDPVKAGYVTDPSAPGNGFAF
                     TASGNGNSNEGHDYNVGSLTEAERLALLEYLKSL"
     gene            3199..3771
                     /locus_tag="VF547_05505"
     CDS             3199..3771
                     /locus_tag="VF547_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX8262312.1"
                     /translation="MLIQTESTPNPATLKFLPGRRVMEAGTRDFATPEDAEASPLAEA
                     IFSTGEVEGVFFGRDFISVTAAPGVDWRQLKPEVLSVLLDHFSSDAPLFRAASAAGIS
                     VPTDEDYGEDPADAQLVAEIRELIETRVRPAVAQDGGDIVYRGFRNGTVYLALHGACS
                     GCPSSTMTLKQGIESLLKHYVPEVEAVEAV"
     gene            3788..>4128
                     /gene="tsaB"
                     /locus_tag="VF547_05510"
     CDS             3788..>4128
                     /gene="tsaB"
                     /locus_tag="VF547_05510"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX8262313.1"
                     /translation="MILIIESATPACSVALADGERLVDERHEIVGRGHAERLVPMIEA
                     MLGGRRPDAILVDCGPGSFTGIRVGLAAAHGLRIGWKVPLTGFSSLAAIAAAAAGVGE
                     IAVALEGGHGQL"
BASE COUNT          687 a         1414 c         1375 g          652 t
ORIGIN      
        1 ccgtagagga acagcggcgg cccgccgacg ccgtctcccc gggcatagcg cggcgccacg
       61 gacctctcgg ggcaaaccgg gcacgcttcg ctgggcgcct cgtcggcgag cggcgccgca
      121 tcggccattt gctccgcgaa agccgcatgc aaggcccgca gcctggcttc gggagcgctg
      181 tcggggcccg gggaccggtc cgcatagccg ctgaggacac gtgcccagcg gtccggctcg
      241 atccgcagcg tgcgcgccgc tcgagccaga tgctcgtcgc cgagggccgg cggcgcctcg
      301 tcgactggac tggctggccg tgccgtcaca gcgcgggtcc gccgtcgcgg ctcaggattc
      361 gccggccggt cccggcacgc cggcataaag ctgctcgacg gtcgcgacat cgcccgggct
      421 gaggccgttg cgctggccct gcaccacgcc gggcggcagc ggctgcttcg ggatcagcac
      481 cgaagtcccc tcgatgaagg cgcaggcggt ctcggaatag tgcatgatcg agccgaagtc
      541 gtaatcgccg atgttgaacg tcggcatcgg ctcatccccg accgcgtcga tggtgaagtt
      601 ctgcaggctg tcctggtcga tcagatcgag gagcaccgtg acgaagtcgt cgcgattgtg
      661 ccggcaatgc tcgtgccaca ggccgacggt gtggccgagc tcgtggatga tggacccgac
      721 tgggcaatct tcggccagac agatcgtctg cttcccgccg cgccgtccga tgtccgagac
      781 cgcgcagccg tcaatccgag ttaccaatac ataatcggtt tccgacgttc gcggcacgaa
      841 gcggatgacc gtatgctcgt tccaatggag gatcgcggcc tctaccgcct ccgcatcggg
      901 cagttgcggg tcgatgacat agggaatggt ccgccccggc caaagatatt gcgcacccgc
      961 aattgccact cccatggctc gacccccgca ttcccgtcgg cacggcaatt ctgcacttca
     1021 acgcagtagc gatcaacggc taatcaccgt tcggctcgct gcgaaaacgg agttgacttc
     1081 gggcttcgaa taggtccgtt tgtgtctccg cgggattgct cggcgccgtc tccctcggga
     1141 gagctcctcc cgggagcggg caagcggggg gtcccatgca ggcagggggg ctgtcatggg
     1201 aaaagctcat aggataagca cgatttcgat ggcgctgagc gccgggctgt tgttctcggt
     1261 cgccggctgc gactggttcg accgtgacga caggccgcct ccccccgccg gatggccgga
     1321 tggcggccag ggctgggatc gcgcgcagcg gctttcgtgg taccaggccg gccagggatc
     1381 gcggctcgtg ccgtggccat gggcgaaagc gctcgagcag aaggatggcg agcagctctt
     1441 cctggccgaa gcgaacatga cccggttcgg cttccttcca ctcgcggaat ccgaggccgc
     1501 gccgaagctc ccgatcggct tcgccatcga tcggcagccc gacgacaagc tcaccttcac
     1561 gaaattgcgc tggtacgagg gtcagcagaa caaggaggag tggctcggcc tcaattgctc
     1621 cgcctgccat accgggcgga tgaatttcga gggcaagacg atcaccgtcg acggcgcccc
     1681 gagcctggtc gacttccaga tgttcatcga ggagttcgac gccgccctcg atgccaccta
     1741 cagccaggac gccaagttcg accggttcgc ggccaaggtg ctcgccggga gggacaaccc
     1801 ggccaatcgc gccaagctcc ggggcgagct gaagaagctg ctcgactggg agaaggccaa
     1861 cgcgaagctc aacgcgacgt cgatgcgata cggcttcggg cggctcgacg ccgtcggcca
     1921 catcttcaac aaggtcgccc agctcgcggt ctacgggacc aacctgccgg cgaagccgaa
     1981 cccggccgac gcgccggtca gctatccctt cctgtgggac atctaccgcc agagccagct
     2041 gcagtgggac ggaatcgtca agacgcagcg cctcaatctc ggcgccggct atctcgacta
     2101 tggcgcgctc gggcgaaacg tcggcgaagt cgtcggcgtg ttcggggacg tcgacctcag
     2161 gccggcgtcg cgcctggcga agaccggctt caactccagc atccaggccg agaacctcaa
     2221 caacctcgag acggtgctgc gcaagctgga ggcgccgaaa tggcccggcg ccctcgaccg
     2281 ggccaaggtc gaccagggca aggcgctcta cagcagcaag ggctgcatcg gctgccacac
     2341 gatcgagccc gccggcgaca atatctacca ggtccacatg actccgctga ccggcagccc
     2401 cacaaccccg aaccggaaca acaccgatcc gtggatggcg tgcaatgcga tcacctacgc
     2461 aagcttcagc gcgaatctga aggacctccc gtcgggctat ttcaagggcg atccgctgcc
     2521 ggcgcaggcg cagctctcgg acatgctgac caccaccgtg atcggcaccc tcgtcgccaa
     2581 gtggaatcag gtgctgaaca gcgccgtcca ggtgttcttc ggcagggtgc ctctgccgaa
     2641 agtcacgcgc gcttccgacc agcagaccga cgccatgaag cgggccgccc ggctggccca
     2701 atgctatgag tcgaagagcc cgctgttcgc ctacaaggcg cggccgctcg acggcgtctg
     2761 ggcgacggcg cccttcctcc acaacggctc ggtgccgaac ctctacgagc tgcttcgcgc
     2821 cccggcggac cgggtgaaga ccttcaacgt cggcacccgc caatacgacc cggtcaaggc
     2881 cggctacgtc accgaccctt cggcgccggg caacggcttc gccttcacgg cctcgggcaa
     2941 cggcaattcg aatgaaggcc acgactacaa tgtcggcagc ctgaccgagg ccgagcggct
     3001 ggccctgctc gaatatctga agtccctctg agggcgacgc gacggcgggg catgtcggcg
     3061 ggcttggccc cttttgtcga ggcggggaaa cgatcgctcc ttggcgaccg atccgggtct
     3121 tcgagcacgg ctgcccagag cctcttgggc tgcgccgggg cgcttgaacc gcgccgtccg
     3181 gcggcccaca tgcgagccat gctgatccag accgaatcga cgcccaaccc ggcgaccctg
     3241 aaattcctgc cgggacggcg ggtgatggag gccgggaccc gtgacttcgc cacccccgag
     3301 gacgccgaag cgtcgccgct ggccgaggcg atcttctcga ccggggaggt ggagggagtc
     3361 ttcttcggcc gcgacttcat ctccgtcacc gccgctcccg gagtcgactg gcggcagctg
     3421 aagccggagg tgctgagcgt cctgctcgac catttctcga gcgacgcgcc gctgttccgg
     3481 gccgcctccg cggccggcat ttcggtcccg accgacgagg attatggcga ggatccggcc
     3541 gacgctcagc tggtcgccga gattcgcgag ctgatcgaga cccgggtccg gccggccgtg
     3601 gctcaggacg gcggcgacat cgtctatcgc ggctttcgca acggcaccgt ctatctcgcc
     3661 ttgcacggcg cctgctcggg ctgtccgtcc tcgaccatga cgttgaagca ggggatcgag
     3721 agcctgctca agcattacgt gcccgaggtc gaggcggtcg aggcggtgtg accgcccgcg
     3781 ggcgatcatg atcctgatca tcgagagcgc cactccggcc tgctcggtcg ctctggccga
     3841 cggcgagcgc ctggtcgatg agcggcacga gatcgtcggc cgcggccatg ccgagcggct
     3901 ggtgccgatg atcgaggcga tgctcggcgg ccgtcgcccc gacgcgatcc tggtcgattg
     3961 cggacccggc agcttcaccg gcatccgcgt cggcctcgcc gccgcccatg gcctccggat
     4021 cggctggaag gttccgctca ccggcttctc ctccttggcg gcgatcgcgg ccgcggcggc
     4081 gggggtcggc gaaatcgcgg ttgcgctcga gggcggacac gggcagct
//
LOCUS       DASVQZ010000199         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_813501, whole genome shotgun sequence.
ACCESSION   DASVQZ010000199 DASVQZ010000000
VERSION     DASVQZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813501"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VF547_05515"
     CDS             complement(<1..862)
                     /locus_tag="VF547_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX8262314.1"
                     /translation="MGVVAQLKSKTENRLGRSDRSAAGRWFWEIDRVLLFFVAVLIAI
                     GLVAVAAASPAAGQRLSGGGVSYAPLYYFYRQLVWIVVALPVMIAVSMLPRTKAKRWC
                     LIGSLVFTGLLFLVPVIGVEVNGARRWLGVGFTQFQPSEFLKPLYIVSIAWMLSLKEQ
                     DASLPVVPLSGICTGLIALLLMQQPNFGETIIFAAAWVMLLTLSGASLRMLGLLALAG
                     LVGIVLAYFFYPVATTRIDAFLFAGAGHDNYQTESALRTLTAGGLFGVGPGAGSHKFH
                     LPEPHTDYIFS"
     gene            complement(862..2139)
                     /gene="murD"
                     /locus_tag="VF547_05520"
     CDS             complement(862..2139)
                     /gene="murD"
                     /locus_tag="VF547_05520"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX8262315.1"
                     /translation="MIASPAFAARKYAVYGLARSGLATVEALLASGAEVMAWDQNEAA
                     RERVKDLGLRMADPLTTSIYGYDGIVVSPGVPLNRHPIAPRAREAKVPLIGDIELFAQ
                     ARASLPPHRVVGITGTNGKSTTTALIHHIVRTAGRPSTMAGNIGFPILGQEPLPEGGI
                     YVLELSSYQIDLTRSLDCDVSVLLNISPDHLDRYDGVDDYAASKARLFAMQSPEHVAV
                     IAAEDDFTRAIAGQIAGRKILVSSDQVKDQSRWPSLQGPHNAQNIAAAAAAAKVLGLP
                     DEAIARGLASYPGLPHRMERVAEKNGVLFVNDSKATNPTSAAPALAAYPAVHWILGGL
                     PKSDDLDACEPFLSHVRAAYTIGEAGPMFARLLREKVPVSECEMLFEAVNEAAERARP
                     GEVVLLSPACASFDQFRDYEARGDAFRAAVEAL"
     gene            complement(2136..3206)
                     /gene="mraY"
                     /locus_tag="VF547_05525"
     CDS             complement(2136..3206)
                     /gene="mraY"
                     /locus_tag="VF547_05525"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX8262316.1"
                     /translation="MFLWIAEQLGFPGVFNLFRYITFRAGAATATALLIGLIIGPKFI
                     GWLRVRQGHGQPIRLDGPETHLAKRGTPTMGGLMILTSLTVSMLLWMDFSNTFLWACL
                     FITVGFGLIGFLDDYDKVTKRSHKGVSARVRLLAEFLVAGIGCTIITWGAGTELYIPF
                     YNGPVVDLGFLYPFFGAFVVVAFGNAVNLTDGLDGLATMPVIIASLAFLVISYVAGNA
                     VFAAYLGIPFVAGAGNLPILCSAIVGAGLAFLWFNAPPAAVFMGDTGSLALGGALGAI
                     AVATQHEIVLGIIGGLFVVEAMSVIIQVFFYKRTGRRIFKMAPIHHHFEQIGWSEATI
                     VIRFWIIAFVLALAGLSTLKLR"
     gene            complement(3206..>3929)
                     /locus_tag="VF547_05530"
     CDS             complement(3206..>3929)
                     /locus_tag="VF547_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX8262317.1"
                     /translation="DSPHRERLAAAARPHAATILTFGLGEGADIRARDVVALPGAGSL
                     VTAVLPEAELTFTVSQPGEHWVSNALAVLGAVEAMGADLAQAGLALADLPGLKGRGER
                     RRIRLDGGEALLIDESYNANPASMEATLKTFGALNVAGRRIAVLGAMRELGETSAGFH
                     AALSEPVVAAGVETAILVGEEMEALAKALGAKVKMAHVPDTATAIELARAEARPGDAI
                     LVKGSNSIGLAALVEALAGGKS"
BASE COUNT          672 a         1301 c         1410 g          546 t
ORIGIN      
        1 ccgagaagat gtagtcggta tgcggctcgg gcaggtggaa cttgtgcgag ccggcgcccg
       61 ggccgactcc gaacagcccg cccgccgtca aggtccgaag cgccgactcg gtctgataat
      121 tgtcgtgccc ggccccggcg aagaggaagg cgtcgatccg ggtcgtcgcc acgggataga
      181 agaaataggc gaggacgatg ccgacgaggc cggcgagggc cagcagcccg agcatgcgca
      241 gcgacgcgcc cgacagggtc agaagcatca cccaggcggc ggcgaagatg atcgtctcgc
      301 cgaaattggg ctgctgcatc aggagcagcg cgatgagccc ggtgcagatg cccgacaggg
      361 gcaccaccgg caggctcgcg tcctgctcct tcagcgacag catccaggcg atgctgacga
      421 tgtagagggg cttgaggaac tccgacggct ggaactgggt gaagccgact ccgagccagc
      481 gccgggcgcc gttcacctcg acgccgatga cgggaacgag gaagagcagg cccgtgaaga
      541 cgagcgagcc gatcaggcac cagcgcttcg ccttggtccg gggaagcatc gatacggcga
      601 tcatcaccgg cagggcgacg acgatccaga ccagctggcg atagaaataa tagaggggcg
      661 cgtagctgac cccaccgccg gagagacgct ggccggcggc cggcgaggcg gcggcgacgg
      721 cgacgaggcc gatcgcgatc agcaccgcga cgaagaagag gaggacccgg tcgatctccc
      781 agaaccagcg gccggcggcg cttcggtccg accggcccag ccggttttcg gtcttgctct
      841 tcagctgcgc gactacgccc atcacaacgc ctccaccgcc gcgcggaagg catcgccccg
      901 cgcttcgtaa tcccggaact ggtcgaacga ggcgcaggcc ggcgacagca gcaccacctc
      961 gccgggcctg gctctctccg ccgcctcgtt caccgcctcg aacagcatct cgcactcgct
     1021 gaccggcacc ttctcgcgaa gcagcctggc gaacatcggc ccggcctcgc cgatcgtgta
     1081 ggcggcgcgg acgtggctca ggaacggctc gcaggcgtcg agatcgtccg atttgggcag
     1141 cccgcccaat atccagtgca cggccgggta ggcggccagg gccggcgccg ccgaggtcgg
     1201 gttggtcgcc ttgctgtcgt tgacgaacag caccccgttc ttctcggcca cccgctccat
     1261 ccggtgcggc aggcccggat agctggcgag accgcgggcg atcgcctcgt ccggcaggcc
     1321 gaggaccttg gccgccgccg ccgcggcggc gatgttctgg gcgttgtgcg ggccctggag
     1381 cgagggccag cgcgactggt ccttgacctg atccgaggac accaggatct tgcggccggc
     1441 gatctgcccg gcgatggcgc gggtgaaatc gtcctcggcc gcgatcaccg cgacatgctc
     1501 gggagactgc atcgcgaaca gccgcgcctt cgaggcggca taatcgtcga cgccgtcgta
     1561 gcggtcgaga tggtcggggc tgatgttgag caggaccgag acgtcgcagt cgaggctcct
     1621 cgtgaggtcg atctggtagg aggagagctc gagcacgtag atcccgcctt ccgggagcgg
     1681 ctcctgcccg aggatcggga agccgatgtt tccggccatg gtcgacggcc ggccggcggt
     1741 ccggacgatg tggtgaatga gcgccgtcgt cgtcgacttg ccgttggtgc cggtgatccc
     1801 gaccacgcgg tgcggcggga gcgaggcgcg cgcctgggcg aacagctcga tgtcgccgat
     1861 caggggaacc ttcgcctccc gcgccctggg cgcgatcgga tggcggttca gcggcactcc
     1921 gggcgagacg acgatgccgt catagccgta gatcgaagtg gtgagcggat cggccatgcg
     1981 caggccgagg tccttcaccc gctcgcgggc cgcctcgttc tggtcccagg ccatcacctc
     2041 cgcgccgctg gcgagcagcg cctcgacggt ggcgagtccg gagcgcgcga ggccgtagac
     2101 cgcgtatttc cttgcggcga aggcggggct cgcgatcatc tcagcttcag ggtcgacagg
     2161 ccggccagcg ccagcacgaa ggcgatgatc cagaagcgga tgacgatggt cgcctcggac
     2221 cagccgatct gctcgaaatg atggtggatc ggcgccatct tgaagattcg ccgcccggtg
     2281 cgcttgtaga agaagacctg gatgatgacg ctcatcgcct cgacgacgaa caggccgccg
     2341 atgatcccga gcacgatctc gtgctgggtc gcgaccgcga tcgcccccag cgcgccgccg
     2401 agcgcgaggc tgccggtgtc gcccatgaag acggcggccg gaggcgcgtt gaaccagagg
     2461 aaggcgaggc cggccccgac gatcgccgag cacaggatgg ggaggttgcc cgcgccggcc
     2521 acgaagggga ttccgagata ggccgcgaag acggcgttgc cggcgacgta ggagatgacc
     2581 aggaaggcga ggctggcgat gatcaccggc atggtggcga ggccgtcgag cccgtcggtc
     2641 aggttgaccg cattgccgaa cgccaccacg acgaaggcgc cgaagaaggg atagaggaac
     2701 cccagatcga cgaccggccc gttgtagaag gggatgtaga gctcggtccc ggcgccccag
     2761 gtgatgatcg tgcagccgat gccggcgacg aggaactcgg caagcagccg cacccgcgcc
     2821 gagacgccct tgtggctgcg cttggtgacc ttgtcgtaat cgtcgaggaa cccgatcagg
     2881 ccgaagccga cggtgatgaa caggcacgcc caaaggaagg tgttcgagaa gtccatccac
     2941 agcagcatcg acacggtgag cgaggtgagg atcatcagcc cgcccatggt cggagtgccg
     3001 cgcttggcga ggtgggtctc cggaccgtcg aggcggatcg gctggccatg gccctggcgg
     3061 acgcgcagcc agccgatgaa cttgggcccg atgatgaggc cgatcaggag cgcggtcgcg
     3121 gtcgccgccc cggccctgaa cgtgatgtag cggaagaggt tgaaaacccc cgggaagccc
     3181 agctgttcgg caatccagag aaacatcagc tcttcccgcc cgccagcgcc tcgacgaggg
     3241 cggcgagtcc gatcgaattg gatcccttga cgagtatcgc gtcgcccggc cgggcctcgg
     3301 ccctcgcaag ctcgatcgcc gtcgccgtgt ccggcacatg cgccattttg accttagcgc
     3361 caagggcttt cgccagcgct tccatctcct cgccgacgag gatcgcggtt tcgacgccgg
     3421 cggccacgac cggctccgac agagcggcgt ggaagccggc gctggtctcg ccgagctcgc
     3481 gcatggcgcc gagcacggcg attcgccgac cggccacgtt gagcgctccg aacgtcttga
     3541 gcgtcgcctc catcgaggcg gggttggcgt tgtagctctc gtcgatgagc agcgcctcgc
     3601 ccccgtcgag gcggatccgc cgccgctcgc cgcggccctt gaggcccggc aggtcggcca
     3661 gcgccagccc ggcctgggcc agatcggcgc ccatcgcctc gaccgcgccg agcacggcca
     3721 gggcgttcga gacccaatgc tcccccggct gggagacggt gaaggtgagc tcggcctcgg
     3781 gcaggaccgc ggtcaccagc gacccggcgc cgggcaaagc gacgacgtcg cgggcgcgga
     3841 tgtcggcgcc ttcgccgagg ccgaaggtga ggatcgtcgc cgcgtgcggc cgggccgcgg
     3901 ccgcgagccg ctcgcgatgc gggctgtcg
//
LOCUS       DASVQZ010000200         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_813558, whole genome shotgun sequence.
ACCESSION   DASVQZ010000200 DASVQZ010000000
VERSION     DASVQZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813558"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VF547_05535"
     CDS             complement(<1..1023)
                     /locus_tag="VF547_05535"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8262318.1"
                     /translation="MKNSVALKFAVSALAIGATMVACKPAAEASRVASASSGTPKADQ
                     QAAALYAKAQAAVQKGAFAEGIGFAERAVELSPRDVGYRMILADLYLKNGRFQSAETT
                     FADVQQLDPTNVRAGFSLALARIALGRPAAAVAELDRLAENASPADIGLAYALAGEPQ
                     RGIELLEPAARAPGATGRVRQNLALAYAIAGDWEKARVTAAQDVSPAELGARLQQWAA
                     FARPDAGATRIAALLGVAPAEDSGQPVRLALAAPEAPATAFAAAGPLPEPASETVSVA
                     SPALAEVAPAAAAAGPEAEPVRTATADLPQWVSERAEAEAREAPPAAAEETRPVYAEA
                     VRALVTP"
     gene            complement(1038..1628)
                     /locus_tag="VF547_05540"
     CDS             complement(1038..1628)
                     /locus_tag="VF547_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262319.1"
                     /translation="MAETKSFASLSSGLLARKGAAKPAMRPQGFGSFGGSLEDLGWND
                     MGHAPGGLHDIHEALPEHVPSSIEALTPAPGGRVQVEPPAVVEQQRELESRLAEPEPE
                     PRAVEPAPQPRPARRAAAKAEVVVLPRSRAVAARAKAAFTLRLDPERHLKLRLACALS
                     RRSAQQLVTEALDDFLDSLPELDALAGHVPEQASQR"
     gene            complement(1642..2361)
                     /locus_tag="VF547_05545"
     CDS             complement(1642..2361)
                     /locus_tag="VF547_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8262320.1"
                     /translation="MRVLALSSQKGGSGKTTLSGHLAVQAERSGAGPVCLIDIDPQGS
                     LADWWNEREAEMPAFAQTTVARLQADLEILRQQGFKLAVIDTPPAITMAIQSVIQVAE
                     LILIPTRPSPHDLRAVGATVDLCERAGKPLIFVVNAATPKARITYEAAVALSQHGTVA
                     PVTLHHRTDYAASMIDGRTVMEVDPNSKSAKEVEELWDYIADRLEKNFRRTIFSAPQQ
                     GYAHPSSARPVGGFGRRVVGQ"
     gene            2555..>3208
                     /locus_tag="VF547_05550"
     CDS             2555..>3208
                     /locus_tag="VF547_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8262321.1"
                     /translation="MHFTGRFLLAASAALLLAAPASADVKSGVDRWMRGDYAGAVAEW
                     RPLADKGNADAQFNLGQAYKLGRGVTANAGVAQSWYEKAARQGHEQAQVNVGLLLYNG
                     GRRQEAMPWIRKAADLGDPRAQYILGTELFNGDLVGKDWPRAYALMLKAADGGVPPAA
                     DNLKAMERFIPIEQRRQGAALAQSMGRGGPTRGGLAAIARPAAPPTAARPSPRPAAPA
                     "
BASE COUNT          420 a         1110 c         1237 g          441 t
ORIGIN      
        1 cggcgtgacc agggcccgga ccgcctcggc atagaccgga cgggtctcct cggcggcggc
       61 gggcggagcc tcgcgggctt ccgcctcggc gcgctcgctg acccattgcg gaaggtcggc
      121 ggtggcggtg cggaccggct cggcctcagg cccggcggcc gcggcggccg gggcgacctc
      181 ggccagggcc ggcgaagcga cggagacggt ctcggacgcc ggctcgggca acggcccggc
      241 ggcggcgaag gcggtggcgg gggcctccgg cgcggcgagc gccagccgca ccggctggcc
      301 cgaatcctcg gccggagcga ccccgagcag ggcggcgatc cgggtggcgc cggcgtccgg
      361 ccgggcgaag gcggcccatt gctggagacg cgccccgagc tcggccggcg agacgtcctg
      421 ggcggcggtg acccgcgcct tctcccagtc gccggcgatg gcataggcga gcgccaggtt
      481 ctgccggacc cggccggtcg cgcccggggc gcgggcggcg ggctcgagca gctcgatgcc
      541 ccgctgcggc tcgccggcga gcgcataggc gaggccgatg tcggcgggcg aggcgttctc
      601 ggccagccgg tcgagctcgg cgacggcggc ggccggacgg ccgagcgcga tccgcgccag
      661 agcgaggctg aacccggcgc gaacgttggt cggatcgagc tgctggacgt ccgcgaaggt
      721 ggtctcggcc gactggaagc ggccgttctt gaggtagagg tcggcgagga tcattcggta
      781 gccgacgtcg cgcgggctga gctcgaccgc gcgctcggcg aagccgatgc cttcggcgaa
      841 ggcgcccttc tggaccgcgg cctgggcctt ggcatagagc gccgccgcct gctgatcggc
      901 cttcggcgtc cccgacgagg ccgaagcgac tcgcgaggct tccgccgccg gtttgcaggc
      961 gaccatggtg gcgccgatcg cgagggcgga cacggcgaac ttgagggcga cgctgttctt
     1021 catcctactc actccgatca acgctgactc gcctgctccg gcacgtgccc ggcaagggca
     1081 tcgagctcgg gcagcgaatc gaggaaatca tcgagggcct cggtgaccag ctgctgcgcc
     1141 gagcggcgcg acagggcgca ggcgaggcgc agcttgaggt gacgctccgg gtcgagccga
     1201 agcgtgaagg ccgccttggc ccgggcggcg acggcgcgcg agcggggcag gaccacgacc
     1261 tcggccttcg ccgcggcccg gcgcgccggc ctcggctgcg gggcgggctc gacggcccgg
     1321 ggctcgggct ccggctcggc gagccgggac tcgagctcgc gctgctgctc gaccaccgcc
     1381 ggcggctcga cctggacccg gccgccgggg gcgggagtca gggcctcgat cgagctcggc
     1441 acatgctcgg gcagagcctc gtggatgtcg tggagcccgc ccggagcgtg gcccatgtcg
     1501 ttccagccca ggtcctcgag gctgccgccg aagctgccga agccctgcgg gcgcatggcc
     1561 ggcttggcgg cgcccttgcg ggccagcagg cccgaggaga gcgaggcgaa agatttggtc
     1621 tcggccatta cgtccctccg cttactggcc gaccacgcga cggccgaagc cgccgacggg
     1681 tcgggcgctg gagggatggg cgtagccctg ctggggggcg gagaagatcg tgcggcggaa
     1741 gttcttctcg agccggtccg cgatatagtc ccaaagctcc tcgacctcct tggcggactt
     1801 gctgttggga tcgacctcca tcaccgttcg gccgtcgatc atcgaggcgg cgtagtcggt
     1861 gcggtggtgg agcgtgaccg gggcgacggt gccgtgctgc gacagcgcga ccgcggcctc
     1921 gtaggtgatc cgcgccttgg gcgtcgcggc gttgacgacg aagatgagcg gcttgccggc
     1981 gcgctcgcaa aggtcgacgg tggcgccgac ggcgcgaagg tcgtgcgggc tcggccgggt
     2041 cgggatcagg atcagctcgg cgacctggat gacgctctgg atcgccatgg tgatggcggg
     2101 gggcgtgtcg atcacggcga gcttgaagcc ctgctggcgc aatatctcga ggtcggcctg
     2161 gagccgggcg accgtggtct gggcgaaggc gggcatctcg gcctcgcgct cgttccacca
     2221 gtcggcgagc gagccttgcg ggtcgatgtc gatcaggcag acggggcccg ccccgctcct
     2281 ttcggcctgg acggcgagat ggccggacaa ggtggtcttg ccggatcctc ccttctgcga
     2341 tgacagtgcc aaaacgcgca tgatttcccc tcgggtcgcg atccttgccg ccggtttgcg
     2401 ctccacttgg ctaaaatccg gttaacgccg gcgccgccgg gcccggcttt tcggggccgt
     2461 ggcggccggc cggaaaaagc ttatgctaag agcgcgttaa gcattttcgg ctagccccgg
     2521 cgccgacgac gacttctcgc tcgggagaaa gtagatgcat ttcaccggcc ggttcctgct
     2581 cgcagcctcg gcggcgctcc tcctggcggc gcccgcctcg gccgacgtca agtccggcgt
     2641 cgaccgctgg atgcgcggcg attatgccgg cgcggtcgcc gaatggcggc cgctcgccga
     2701 caagggcaat gccgacgccc agttcaacct cggccaggcc tacaagctcg gccgcggcgt
     2761 gacggccaat gccggtgtcg cccagagctg gtacgagaag gccgcccggc agggccacga
     2821 gcaggcccag gtcaatgtcg gcctgctgct ctacaatggc ggccgccggc aggaggcgat
     2881 gccgtggatc cgcaaggccg ccgatctcgg cgacccgcgc gcccagtaca tattgggcac
     2941 cgagctcttc aacggcgacc tcgtcggcaa ggactggccg cgcgcctacg cgctgatgct
     3001 gaaggccgcc gacggggggg ttccgcccgc cgccgacaat ctcaaggcga tggagcgctt
     3061 catcccgatc gagcagcgcc gccagggcgc cgccctcgcc cagtcgatgg gccggggcgg
     3121 gccgacccgc ggcggcctcg cggcgatcgc ccgccccgcg gctccgccga cggcggcgcg
     3181 gccgtctcct cggcccgcgg cgccggcg
//
LOCUS       DASVQZ010000201         4206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_429092, whole genome shotgun sequence.
ACCESSION   DASVQZ010000201 DASVQZ010000000
VERSION     DASVQZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429092"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..947)
                     /gene="serB"
                     /locus_tag="VF547_05555"
     CDS             complement(69..947)
                     /gene="serB"
                     /locus_tag="VF547_05555"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX8262322.1"
                     /translation="MFIATLRAAERLAGGDISAASDALAAAGIAPAGRSWIEDDKACD
                     LLFSAAPGPARAALEGLLPGVDVVVQGEAARRRKLLVADMDSTMITVECIDELADYAG
                     LKAEVAAVTERAMRGEIAFEAALAERVALLAGLDEAAIDRCRTERVRMTPGGRALVRT
                     MKRYGAVTILVSGGFTRFAEPVGAELGFDRIIANRLGAMAGVLDGTVAPPILGAEGKR
                     LALLDAVAERGLRIDETLAVGDGANDVPMLREAGLGVAYRAKPAAAAAADASIVHNDL
                     GALLYAQGWASAEWER"
     gene            940..1872
                     /gene="miaA"
                     /locus_tag="VF547_05560"
     CDS             940..1872
                     /gene="miaA"
                     /locus_tag="VF547_05560"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX8262323.1"
                     /translation="MNISSSSGKRLALIAGPTASGKSALALALAERSGGTVINADSAQ
                     VYADLRLISARPGPEEEARAPHRLYGYRDGALPCSAADWAADARRAIAAAQAEGRLPI
                     LAGGTGLYIRTLLDGIAPVPQIDPAIREAVRSFSVAHNYAALQVEDPAAAARLRPTDT
                     SRVARALEVVRSTGRPLQEWQRQRRTGGLAGEVDLAPLVLLPERGRLYARCDARFDEM
                     MSDEGIEEVRALLDRGLNPLLPVMRAIGVREIAAFLRGELSRQEALEAGRTATRQYAK
                     RQYTWFSRQTPADWPRFRDAVDDPADLFHFFRSG"
     gene            1956..2942
                     /gene="ilvC"
                     /locus_tag="VF547_05565"
     CDS             1956..2942
                     /gene="ilvC"
                     /locus_tag="VF547_05565"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX8262324.1"
                     /translation="MRVYLDADADLRLIRSRKVAVLGYGNQGRAQALNLRDSGVAVTV
                     GLPEGSASRARAAADGFEVATAAQACRNADLAVLLAADETHGRIYGEEVAPNLREGGA
                     LLFAHGLSIRFGLVRPRPDLDVLLVAPKGPGTALRREFERGGGLVALFAVNQDPRGEA
                     QALALSYAAAIGSGRVGILETSFAEECEADLFNEQAVLWGAIPELIHAGFETLVDAGF
                     APEIAYLECLTEVKLIADLIYERGIAGMREAISNTAEFGALKGGTRIVTADTRTEMRR
                     ILREVRSGSFVKELIADAEAGYPRLEASRAEAAAHPIEAAAARLRALEAKRG"
     gene            2939..3289
                     /locus_tag="VF547_05570"
     CDS             2939..3289
                     /locus_tag="VF547_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262325.1"
                     /translation="MSEGRVWRPRRPFEYAFLALLLAGAAWCVWGVWHDRRLPGRFER
                     IHLGMDRKAVEAVLGGPDWEGGCGAYVPSLPRADCARELAYASAFAPLVPTYYLVQLD
                     RAGRVVEAEAVRAP"
     gene            3533..>4206
                     /locus_tag="VF547_05575"
     CDS             3533..>4206
                     /locus_tag="VF547_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8262326.1"
                     /translation="MPEASRPRPAWLPPPDGARDPRIEDPTNLWVVHLAGRLLLPLAL
                     KARISANAVSVAGLGIGTAAAFAFHGWSDPGLATLGLLLAVGWLVLDGLDGMVARATG
                     SASALGRFLDGLCDHGVFLILYLALGFAIDTPQAYVVGTIAGLVHGVQANLFESERAR
                     YHRRVRGDPGAGAPPPSPNAVVRAYDRLAGSLDRLAGRFDRALAAAPDRRGLAAAYGE
                     RAAPAL"
BASE COUNT          526 a         1549 c         1521 g          610 t
ORIGIN      
        1 ttccgctctg cgtggagccc cggcctatct tccattttcg aatattaggt gccgggcacc
       61 gatggctgtc atcgctccca ttcggcgctc gcccagccct gggcgtagag gagggcgccg
      121 aggtcgttgt ggacgatgct cgcgtcggcg gcggcggcgg cggcgggctt ggcgcgatag
      181 gcgacgccca gcccggcttc gcggagcatc ggcacgtcgt tggcgccgtc gccgacggcg
      241 agcgtctcgt cgatgcggag cccgcgctcg gcgaccgcgt cgagcagcgc caggcgcttg
      301 ccctccgctc cgaggatcgg gggggcgacc gtgccgtcca agacaccggc cattgcaccc
      361 agtcgattgg cgatgatccg gtcgaagccg agctcggcgc cgaccggctc ggcgaagcgg
      421 gtgaagccgc ccgagacgag gatcgtgacg gctccgtaac gtttcatcgt ccggaccagc
      481 gcccggccgc cgggagtcat ccggacccgc tcggtccggc accggtcgat cgcggcctcg
      541 tcgagcccgg cgagcagcgc gacccgctcc gccagcgccg cctcgaacgc gatctcgccc
      601 cgcatcgccc gctcggtcac cgccgccacc tcggccttca gcccggcata gtccgcgagc
      661 tcgtcgatgc attcgacggt gatcatcgtc gaatccatgt cggcgacgag cagcttgcgg
      721 cgccgcgccg cctcgccctg gacgacgacg tcgacgccgg gcagaaggcc ttcgagcgcg
      781 gcccgggcgg ggcccggagc ggccgagaag agcaggtcgc aggccttgtc gtcctcgatc
      841 cagctgcggc cggcgggcgc gatcccggcg gcggcgagcg cgtccgaggc cgcggaaata
      901 tcgccgcccg cgagccgctc tgctgctctc agggtggcga tgaacatctc ttcctcttcg
      961 ggcaagcggc tggcgcttat cgcggggccg accgccagcg gcaagtcggc tctggcgctc
     1021 gccctggccg agcgctccgg cgggacggtg atcaacgccg acagcgcgca ggtctatgcg
     1081 gatctgcgcc tgatctcggc ccgccccggc ccggaggagg aggcccgcgc gccgcaccgc
     1141 ctctacggct atcgcgacgg cgccctgccc tgctcggccg cggattgggc cgccgacgcg
     1201 cgccgcgcca ttgcggcggc ccaggcggag ggacggctgc cgatcctggc cggcggaacc
     1261 ggcctctaca tccggaccct gctcgacggg atcgcgcccg tcccccagat cgacccggcg
     1321 atccgcgagg cggtccgctc gttcagcgtc gcgcataatt atgcggcgct gcaggtcgag
     1381 gacccggcgg cggcggcccg cctccggccg accgacacgt cgcgggtggc gcgcgcgctg
     1441 gaagtggtcc gctcgaccgg ccgcccgctg caggaatggc agagacagcg ccggaccggc
     1501 ggcctcgccg gcgaggtcga cctcgccccc ctcgtcctcc tgcccgagcg cgggcggctc
     1561 tacgcgcgct gcgacgcgcg cttcgacgag atgatgtcgg acgagggaat cgaggaggtc
     1621 cgagcactcc tcgaccgcgg gctcaatccg ctgctgccgg tgatgcgcgc gatcggggtg
     1681 cgggagatcg ccgcctttct gcgcggcgag ctgagccggc aggaggcgct cgaggcgggg
     1741 cgcaccgcca cccgccaata tgccaagcgc caatatacct ggttctcgcg ccagactccc
     1801 gccgattggc cccgcttccg ggacgcggtg gatgatcctg ccgacctctt ccacttcttc
     1861 cgttcgggct gagcctgtcg aagcccattc cttcgcccgc ccgaagtgaa ggaaagccct
     1921 tcgacaagct cagggcgaag ggttagtccg gtccgatgcg ggtctatctc gatgccgacg
     1981 ccgatctgcg cctgatccga agccgcaagg tcgccgtcct cggctacggc aaccagggcc
     2041 gggcgcaggc gctcaacctt cgcgacagcg gcgtcgcggt caccgtcggg ctgccggagg
     2101 gctcggcgag ccgcgcccgg gccgccgccg acggattcga ggtcgcgacg gcggcgcagg
     2161 cctgccggaa cgccgatctg gccgtgcttc tcgcggcgga cgaaacccac ggccggatct
     2221 atggcgagga ggtggcgccg aacctcaggg agggcggcgc cctgctcttc gcacacggtc
     2281 tcagcatccg cttcggcctg gtccggccgc gccccgacct cgacgtcctc ctggtcgctc
     2341 cgaaggggcc gggaacggcg ctccggcgcg aattcgagcg cggcggcggc ctcgtcgcct
     2401 tgttcgccgt gaaccaggat ccgcgcggcg aagcgcaggc gctggccctg tcctatgcgg
     2461 cggcgatcgg cagcggccgg gtcggaatcc tcgagaccag cttcgccgag gaatgcgagg
     2521 ccgacctgtt caacgagcag gcggtgctct ggggggcgat tcccgagctg atccacgccg
     2581 gcttcgagac cctggtcgac gccggcttcg cgccggagat cgcctatctc gaatgcctca
     2641 ccgaggtgaa gctgatcgcc gacctgatct acgagcgcgg aatcgccggg atgcgcgagg
     2701 cgatctccaa cacggccgag ttcggggcgc tgaagggcgg aacccggatc gtcaccgccg
     2761 acacccggac cgagatgcgc cggatcctcc gcgaggtgag gagcgggagc ttcgtcaagg
     2821 agctgatcgc cgacgccgag gccggctatc cgcggctcga ggcgagccgg gccgaggccg
     2881 ccgcccaccc gatcgaggcg gcggcggcgc ggcttcgggc gctggaggcg aagcgcggat
     2941 gagcgagggc cgcgtgtgga ggccgcgccg cccgttcgaa tatgccttcc tggccctgct
     3001 cctcgccggc gcggcctggt gcgtctgggg cgtctggcac gaccgccggc tgccggggcg
     3061 cttcgagcgg atccatctcg gcatggaccg caaggcggtg gaggcggtgc tcggcggccc
     3121 ggactgggag ggcggctgcg gcgcctatgt gccgtcgctg ccccgggccg actgcgcgcg
     3181 cgagctcgcc tatgcctccg ccttcgcgcc gctcgtcccg acctattacc tggtccagct
     3241 cgaccgcgcc ggccgggtgg tggaggcgga ggcggtgcgc gcgccctgac ggggcccgcg
     3301 gacggggctc cctttctggt caatcggtgc cggatacgca ttttcgcccg gggcgccctt
     3361 tccggcgcaa ttcctgaggg gactgtgcga aggacggcgg aggggctctt tcagactccg
     3421 aagagcccct ccaccgcgct tcgcgcggtc cccctccccg agaaatctcg gggaggaatt
     3481 tggggggacg gcgccgattg accgcggtgg cggcttgcgc tttaggcgcg ccatgcccga
     3541 agcgtcgcgt ccccgtcccg cctggctgcc gccgcccgac ggcgcccgcg acccgcgaat
     3601 cgaggatccg accaacctgt gggtcgtcca cctcgccggc cggctgcttc tgccgctggc
     3661 gctcaaggcc cggatctccg ccaatgcggt gtcggtcgcc gggctcggaa tcggcaccgc
     3721 cgccgccttc gctttccatg gctggagcga tccggggctg gcgaccctgg gcttgctgct
     3781 cgccgtcggc tggctggtcc tcgacgggct cgacgggatg gtcgctcggg cgaccggcag
     3841 cgccagcgcg ctcggccgct tcctcgacgg cctgtgcgac cacggcgtct tcctgatcct
     3901 ctatctggcg ctcggcttcg ccatcgacac tcctcaggcc tatgtggtcg gcacgatcgc
     3961 cggcctggtg cacggcgtcc aggccaatct gttcgagagc gagcgcgccc gctaccaccg
     4021 ccgggtgcgg ggcgatcccg gcgccggcgc accgccgccc tcgcccaacg cggtcgtccg
     4081 cgcctacgac cggctggcgg gctcgctcga ccgcctcgcc ggccgcttcg accgggcgct
     4141 ggcggcggcg cccgaccggc gcggactggc cgccgcctat ggcgagcggg ccgcgccggc
     4201 gctgag
//
LOCUS       DASVQZ010000202         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_620678, whole genome shotgun sequence.
ACCESSION   DASVQZ010000202 DASVQZ010000000
VERSION     DASVQZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620678"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2533)
                     /locus_tag="VF547_05580"
     CDS             complement(<1..2533)
                     /locus_tag="VF547_05580"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX8262327.1"
                     /translation="MSGDFSRDTFRPGGDRTFVRMQQGRLLTDADWNEQGDILSEALR
                     ETTADVVGQSGFPEDNPGFALVFAGTGNGIAIQPGEAYVDGIRVAAPTLRIALTRSSG
                     AGANTKWRLDRGARVAVGDLLTVAGQPPVRVSQLFTDSDGKQIFQCAAALSAANNVEA
                     QRAYSVDSQPFLPPPPPPQLPTQNGDYVAYLDVWERPIGAIEDPLIRETAFGGPDTAM
                     RDQLVWQVKFARQSDLAAVGALTPPATCKSFGPTWSPLGTATAPGAMAARAEASSAAN
                     NPCVLPSTGGYRSLENHLYRVEVHNGGRTADNAVTIKWSRDNGIHRTSYSQIVDGAIM
                     VDALGRDDESALNTNDWVEILDEARVLSGRPGFFGQIKEVNGNGVSLLQLLDPDTLAA
                     LVANDLPDTAKLPPKGQIRRWEGGQPQKVVVNSWMKLENGVEVQFAPGRYATQDFWTI
                     PARTGTADVEWPRDPAVVGAPAFVPAMGIRHHYCALAIAVLGPGGWQLTDCRSLFAPL
                     TRQNAFHYLGGDGQEAMPNPLAPSTPIPLGAPLRVGFVRGRTPVAGARVRFTILSGGG
                     KFATGPNPQVVTTGPDGVAGIAWSVGAQVKTQQVLAELIDPQDQATQLPVIFTANLSQ
                     AEDTSFNPANVPALAGANTVQAAIEKLAGMQQVGCATRIIVEGSDWVSVLKGLKPGED
                     AAVCFQRGTYETQETVLLANLGHVTLSGAGGGTRIVGRRRECAIEFTGCASVTVHDLD
                     ISTPDGTGAIENFQRRKGSLTIVECGSADVHDVSLSCGAGVEAERTCLTIRGNLQAPI
                     PSVRVQRNRISAGYAQDGILVTDCVRGVIADNELLVVPRPPSRAAG"
     gene            complement(2544..>3105)
                     /locus_tag="VF547_05585"
     CDS             complement(2544..>3105)
                     /locus_tag="VF547_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262328.1"
                     /translation="GLGLFDSIVGEDRTSGEDPESMSLVVLAPAADLELARSTLFGRC
                     EVRAIEAENSILVGRADASHRQTGCIRFCYAPLSSRVPRRYRCAPDLSLDSEKVRLGR
                     ELTPVERLQVANGTVPRFTASRFGASAFGQLSLACPAAVRVGAEGGAEMGAGFGAGEP
                     FRRANLADAMQEYLPFGLEAATLFLS"
BASE COUNT          429 a         1025 c         1173 g          478 t
ORIGIN      
        1 tgccggcggc ccgcgagggc ggccgcggca cgaccaggag ctcgttgtcg gcgatcacgc
       61 cgcgaacgca gtcggtgacg aggatgccgt cctgggcata gccggccgag atccggttgc
      121 gctggacccg caccgacggg atcggcgcct gcaggttgcc gcgaatggtg aggcaggtgc
      181 gctccgcttc cacgccggcg ccgcagctga ggctgacgtc gtggacgtcg gccgagccgc
      241 actcgacgat ggtgagcgaa cccttgcgcc gctggaagtt ctcgatcgcg ccggtgccgt
      301 cgggggtcga tatgtcgagg tcgtggacgg tgaccgaagc gcagccggtg aactcgatcg
      361 cgcattcccg ccgcctgccg acgattcgag tgcctccgcc ggcgccggac agggtgacgt
      421 ggcccagatt ggcgagcagc acggtttcct gggtctcgta ggtgccgcgc tggaagcaga
      481 cggccgcgtc ctcgccgggc ttcaggccct tgaggacgct cacccagtcg ctgccttcga
      541 cgatgatccg ggtggcgcag ccgacctgct gcatgccggc gagcttctcg atcgcggcct
      601 ggaccgtgtt cgccccggcc agcgccggca cgttggccgg attgaaggag gtgtcctcgg
      661 cctggctcag attggcggtg aaaatgaccg ggagttgggt cgcctggtcc tgcgggtcga
      721 tcagctccgc gaggacctgc tgggtcttta cctgggcacc gaccgaccag gcgatgccgg
      781 cgacgccgtc gggcccggtc gtcacgacct ggggattggg cccggtggcg aatttgccgc
      841 ctcccgacag gatcgtgaag cgcacccttg cgccggccac cggagtgcgg ccgcgtacga
      901 agccgacccg gagcggtgcg ccgagcggga tcggcgtgct gggcgcgagc gggttgggca
      961 tcgcctcctg gccgtcgccg ccgagataat ggaaggcgtt ctggcgggtc agcggcgcga
     1021 acaggctgcg gcagtcggtc agctgccagc cgccagggcc gaggacggcg atggcgaggg
     1081 cgcaataatg gtggcggatg cccattgcgg gcacgaaggc gggcgctccg acgacggcgg
     1141 gatcgcgcgg ccattcgacg tcggccgttc cggtccgcgc cgggatcgtc cagaaatcct
     1201 gggtcgcgta gcgccccggc gcgaactgga cctcgacccc gttctcgagc ttcatccagc
     1261 tgttgacgac gaccttctgg ggctggccgc cttcccagcg gcggatctgc cccttgggcg
     1321 ggagcttggc cgtgtcgggc aggtcgttcg cgaccagcgc ggcgagcgtg tccggatcga
     1381 gcagctggag gaggctgacg ccgttgccgt tgacctcctt gatctggccg aagaagccgg
     1441 gacggcccga aagcactcgg gcttcgtcga ggatctcgac ccagtcgttg gtgttcagcg
     1501 cgctttcgtc gtcgcggccg agcgcgtcga ccatgatcgc gccgtcgacg atctgggaat
     1561 aggaggttcg gtgaatgccg ttgtcgcggc tccatttgat cgtcaccgca ttgtcggcgg
     1621 tgcggccgcc gttgtggacc tcgacccggt agagatggtt ctctaggctg cgatagccac
     1681 cggtcgaggg caggacgcag ggattgttcg ctgcgctcga cgcctcggcg cgggcggcca
     1741 tcgcgccggg ggcggtggcc gtcccgagcg gcgaccaggt cgggccgaag ctcttgcagg
     1801 tggccggcgg cgtgagggcg ccgacggcgg cgaggtcgga ctgcctggcg aacttcacct
     1861 gccagacgag ctggtcccgc atcgcggtgt cggggccgcc gaacgcggtc tcgcggatca
     1921 gcggatcctc gatcgcgccg atcggccgct cccagacgtc gagataggcg acatagtcgc
     1981 cgttctgggt cggcagctgg ggcggcgggg gcggcggcag gaagggctgg ctgtcgacgc
     2041 tgtaggcgcg ctgcgcctcg acattgttgg cggcggagag cgcggcggcg cattggaaga
     2101 tctgcttgcc gtccgagtcg gtgaacagct gcgagacccg caccggcggc tggccggcga
     2161 cggtgagcag gtcgccgacc gccacgcgcg cgcctcggtc gagccgccat ttggtgttgg
     2221 cgccggcgcc cgagcttcgg gtcagggcga ttcgaagggt cggcgcggcc acccggatac
     2281 cgtcgacata ggcttcgccc ggctggatgg cgatgccgtt cccggtcccc gcgaagacga
     2341 gggcgaagcc cggattgtcc tccggaaatc cgctctggcc gacgacgtcg gcggtggttt
     2401 cgcggagggc ctcgctgagg atatcgccct gctcgttcca gtcggcgtcg gtcagcagcc
     2461 ggccctgctg catgcgcacg aaggtgcggt cgccgccggg ccggaaggtg tcgcgcgaga
     2521 aatccccact catgtcgctt tcctcaactt aggaacaagg ttgcggcctc gaggccgaag
     2581 ggcagatatt cctgcatcgc gtccgccaga ttggcgcggc ggaacggctc gcccgcgccg
     2641 aaaccggcgc ccatctcggc gcccccctcg gcgccgaccc ggaccgccgc cgggcaggcg
     2701 agcgagagct ggccgaaggc gctcgcgccg aaccggctcg cggtgaagcg cggcaccgtc
     2761 ccgttcgcca cctggagacg ctcgaccggg gtcagttccc gcccgagccg gaccttctcc
     2821 gagtcgagcg agaggtcggg cgcgcagcgg tagcggcgcg gcaccctgga cgagagcggc
     2881 gcgtagcaga agcgaatgca gccggtctgg cggtgcgagg cgtccgcgcg gccgacgagg
     2941 atgctgttct cggcctcgat cgcccgcacc tcgcagcggc cgaacagggt gcttcgggcg
     3001 agctccaggt cggcggcggg cgccagcacg acgaggctca tcgactcggg gtcctcgccc
     3061 gacgtgcggt cctcgccgac gatcgaatcg aagaggccga gcccg
//
LOCUS       DASVQZ010000203         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_239069, whole genome shotgun sequence.
ACCESSION   DASVQZ010000203 DASVQZ010000000
VERSION     DASVQZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239069"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VF547_05590"
     CDS             <1..1702
                     /locus_tag="VF547_05590"
                     /inference="COORDINATES: protein motif:HMM:NF017142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262329.1"
                     /translation="LKPVAYPALAAAALLLTAAKPPPEKPISYKMTPVLQADGSRALD
                     VEMRLRGDQDGETDIFLPSVWAGSSELWRHATRLRISGARSLAGSYEHPVIRHRPGAR
                     LKARYRIVSAYSEDPGFAYEKARPVVRPDWFFVHGETVFATPGADSARPARFKWGKLP
                     KGWTVASDLDHLQGKPTTLANLVNTVAIGGSKLRVVRRDLRGAQLRVAVLGEWSFTPD
                     QLADTVAPIVAAEDAFWEERSSPFLVAMAPLGDLPSGLFYAGTGRTDAFSIASTGAFE
                     LKQAVRFLAHEYMHSWVPIELGAMPEENEARDYWFSEGFNDYLAAKVLLRAGIWSLAD
                     WAADKNETLLRYGTSPAKTVRGDEIAARFWTDQNVQQVSYDRGHLLAAKLDAEIAAAG
                     GGKSLESVLRVQRRAAQSSSELATDLFRKTLREQTGIDIESDLERYARGGEPLLLPAD
                     LFGDCGRLLAERRREFDRGYDAAATRRSDGVITGVTPDGPAYAAGLRDGMRLIRRESG
                     KIGDSTAELAYRVADEAGERVVRYLPQGKAEFEVQRFELTASGPEQEALCRARLGGAA
                     "
     gene            1699..2874
                     /gene="purT"
                     /locus_tag="VF547_05595"
     CDS             1699..2874
                     /gene="purT"
                     /locus_tag="VF547_05595"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEX8262330.1"
                     /translation="MTFTARILLLGSGELGREFTISAKRLGAFVIACDSYAGAPAMQV
                     ADEAEVFPMLDGDRLRSAIQRWRPDYLVPEVEAIRTEVLAEVEAEGTVVIPSARATMM
                     TMNRDSIRELAAGRLGLRTSRYRFAESLEEVEAAALHAGFPCVVKPVMSSSGKGQRTV
                     EAAAGLGAAWDYAVANMRGDRARVIVEELVRFDYEITLLTVRTRDGILFCPPIGHRQE
                     RGDYRESWQPTPMSKAALRDAQDMARRVVEDLGGHGLYGVEFFVAGDEVIFSELSPRP
                     HDTGMVTLISQNLSEFDLHARAVMGLPIPHIALTAPAAASAVILADREAERFRFEGVA
                     DALALGGEGAEVDVRIFGKPVTRPYRRMGVALATGATAEEARALAARAAQAVTIAYD"
     gene            complement(3013..3624)
                     /locus_tag="VF547_05600"
     CDS             complement(3013..3624)
                     /locus_tag="VF547_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262331.1"
                     /translation="MRRWTAGVLAACLLAPGFAAETRPRRDLPTQVAHTPWLALRFEQ
                     DSKPARLVNRDLRTTEVELAARPFRVVFPKNAPDVAYQLCAWTDDSIFRQLVSRATDR
                     DPDRGSEHPCFGLGTGMADTDAGSGKLMLNDEGHNYLIDLRLGPDPDHPFVYYSALWA
                     RGVGDMPIGKARRPIYVIAFHDRDRDDLLDNGEYDFLVLKFRR"
     gene            3835..>4257
                     /locus_tag="VF547_05605"
     CDS             3835..>4257
                     /locus_tag="VF547_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEX8262332.1"
                     /translation="MKKCLAFGVSLIAFAAAPAAARHGHDCLDEACAIQALIQPQPEG
                     GAAATVEAKRYGSWGLDTAGMDRSAKPGDDFYAYANGTWAKTTQIPADRSSYGAFAVL
                     RELSEARLKTLVEGYRPGAAGDEGKVAELYRGFLDEAAA"
BASE COUNT          644 a         1514 c         1474 g          625 t
ORIGIN      
        1 cttgaaacca gtcgcttacc ccgcgctcgc cgccgcggcc ctgctcctga cggccgccaa
       61 gccgcctccc gaaaagccca tctcctacaa gatgaccccc gtcctccagg ccgacggcag
      121 ccgcgcgctc gacgtcgaga tgcgactgcg cggcgaccag gatggcgaga ccgacatctt
      181 ccttccgagc gtctgggccg gatcgagcga gctgtggcgc cacgcgacca ggctcaggat
      241 aagcggcgcc cgaagcctcg ccggcagcta cgagcacccc gtcatccgcc accgcccggg
      301 cgcccgcctc aaggcgcgct accggatcgt ctccgcctat tccgaagacc cgggcttcgc
      361 ctacgagaag gcgcgcccgg tggttcggcc cgactggttc ttcgtccacg gcgaaaccgt
      421 cttcgccacg ccgggcgccg actccgcgcg tccggcccgg ttcaaatggg gcaagctccc
      481 caagggctgg accgtcgcct ccgacctcga ccatctgcag ggcaagccga ccacgctcgc
      541 caatctggtc aacacggtgg cgatcggcgg atcgaagctg cgggtggtcc gccgcgacct
      601 gcgcggcgcg cagctccggg tcgcggtgct cggcgagtgg agcttcacgc ccgaccagct
      661 cgccgacacg gtcgctccga tcgtcgcggc cgaggacgcc ttctgggagg agcgcagctc
      721 gcccttcctc gtcgccatgg cgccgctcgg cgatctgcct tcgggcctct tctacgcggg
      781 caccggccga accgacgcct tctcgatcgc ctcgaccgga gctttcgagc tgaagcaggc
      841 ggtccgcttc ctcgcccacg aatatatgca ctcctgggtg ccgatcgagc tcggcgcgat
      901 gccggaggag aatgaagccc gggattattg gttcagcgaa ggcttcaacg actatctcgc
      961 cgccaaggtg ctgctgcggg ccggaatctg gtcgctggcc gactgggcgg cggacaagaa
     1021 cgagaccttg cttcgctacg gcacctcccc ggcgaagacc gtcaggggcg acgagatcgc
     1081 ggcccgcttc tggaccgacc agaacgtcca gcaggtgagc tacgaccgcg gccacctgct
     1141 cgccgccaag ctcgacgcgg agatcgccgc ggccggcggc ggcaagagtc tcgagagcgt
     1201 gctccgggtc cagcggcggg ccgcccagtc cagctccgag ctcgcgaccg acctgttccg
     1261 aaagacgctg cgcgagcaga ccgggatcga catcgagtcg gatctggagc gctacgcgcg
     1321 cggcggcgag cctctgctgc tgccggcgga cctgttcggc gactgcggcc ggctcctcgc
     1381 cgagcggcgc cgggaattcg accgcggcta cgacgccgcg gccacccgcc gaagcgacgg
     1441 cgtcatcacg ggcgtgacgc cggacggtcc ggcctacgcc gccggccttc gcgacgggat
     1501 gcgcctcatc cgccgcgaga gcgggaagat cggggattcg acggccgagc tggcctaccg
     1561 ggtcgccgac gaggcgggcg agcgggtggt ccgctacctg ccgcagggca aagccgagtt
     1621 cgaggtccag cgcttcgagc tgaccgcgtc cggcccggag caggaggcgc tctgccgggc
     1681 ccggctcggc ggcgcggcgt gaccttcacc gcccggatcc tgctgctggg ctcgggcgag
     1741 ctgggccggg aattcacgat ctccgccaaa aggctcggcg ccttcgtcat cgcctgcgac
     1801 agctatgcgg gcgcccccgc catgcaggtc gccgacgagg ccgaggtctt cccgatgctc
     1861 gacggcgacc ggctgcggtc ggcgatccag cgctggcgtc ccgattatct ggtgcccgag
     1921 gtcgaggcga tccgaaccga ggtcctcgcc gaagtcgagg ccgagggcac cgtcgtcatc
     1981 ccctccgctc gagcgacgat gatgacgatg aaccgggatt cgatccgcga gctggcggcc
     2041 ggcaggctcg gcctgcggac ctcgcgctac cgcttcgccg agagcctcga ggaggtggag
     2101 gccgccgcct tgcacgccgg cttcccctgc gtcgtcaagc cggtcatgtc gtcgtccggc
     2161 aagggccagc gcaccgtcga ggccgccgcc gggctgggcg ccgcctggga ctatgccgtc
     2221 gccaacatgc ggggcgaccg ggcccgggtg atcgtcgagg agctcgtccg cttcgactat
     2281 gagatcaccc tgctcaccgt ccggacccgc gacgggatcc tgttctgccc gcccatcggg
     2341 caccgccagg agcgcggcga ctaccgggag agctggcagc cgaccccgat gtcgaaggcc
     2401 gcgcttcgcg acgcccagga catggcgcgc agggtggtcg aggatctcgg cggccatggc
     2461 ctctacgggg tcgagttctt cgtcgccggc gacgaagtga tcttctccga gctctcgccg
     2521 cgtccgcacg acaccggaat ggtcaccctc atctcccaga acctctccga attcgacctc
     2581 cacgcccggg cggtcatggg cctgccgatc ccgcacatcg ccctcacggc cccggccgcc
     2641 gcgtccgcgg tcatcctcgc cgatcgcgaa gcggagcgct tccgcttcga gggagtcgcc
     2701 gacgcgctgg cgctgggcgg cgaaggcgcc gaggtggacg tccggatctt cggcaagccg
     2761 gtgacgaggc cctaccgccg gatgggcgtg gctctggcga ccggggcgac ggcggaggaa
     2821 gcccgggcac tggcggcccg cgcggcgcag gccgtgacga tcgcgtacga ctgacgaatt
     2881 cctccccgcg atttcgcggg gaggggacca tgcgaagcat ggtggagggg ttctccggcg
     2941 gctccaagaa cccctccacc gcacttcgtg cggtccccct ccccggcaaa tgccggggag
     3001 gaattgaagg cctcagcgtc tgaacttcag caccaggaag tcatattcgc cattgtcgag
     3061 caggtcgtcg cgatccctat cgtgaaaggc gatgacgtat atcggccgac gcgccttccc
     3121 gatcggcatg tcgccgacgc cgcgggccca taaggcggaa taatagacga agggatggtc
     3181 cgggtcgggc cccagcctga ggtcgatcag gtaattatgg ccttcgtcgt tcagcatgag
     3241 ctttccggag ccggcgtcgg tgtcggccat gccggtgccg agaccgaagc agggatgctc
     3301 cgatccccgg tcggggtcgc gatcggttgc ccgggagacc agctggcgga agatcgaatc
     3361 gtcggtccag gcgcagagct gataggcgac gtcgggagcg ttcttgggga agaccactcg
     3421 gaacggacgt gccgccagct cgacctcggt cgtcctcagg tcgcggttga cgaggcgggc
     3481 gggcttcgag tcctgctcga agcggagcgc gagccagggc gtgtgcgcaa cctgggtcgg
     3541 caaatcccgc cgggggcgag tctcggctgc gaaaccgggg gcgaggaggc acgccgcgag
     3601 cacgccggcc gtccatcttc tcatggcttt ccccccgtcc ctccgggcga cgataggccg
     3661 ggccggccgg ctgggcaatt gccggagcct attcttccac ggaagccgga cgttccggac
     3721 ctatgaaact ccgcggcggc ggcgcgccgc atttgcgatc tggccgcgcc cgcgctatct
     3781 cccggctcca gccggccaag cgtcggaaat ccttcgatat tcggagtgga ccagatgaag
     3841 aagtgtctcg ctttcggtgt gtcgctgatc gcctttgccg ccgccccggc ggcggcccgg
     3901 cacggccatg actgcctgga cgaggcgtgc gcgatccagg cgctgatcca gccgcagccg
     3961 gagggcgggg cggcggcgac ggtcgaggcg aagcgctacg gcagctgggg cctcgacacc
     4021 gccggaatgg accggtcggc caagcccggc gacgatttct acgcttacgc caacgggacc
     4081 tgggcgaaga ccacccagat ccccgccgac cgttcgagct acggcgcgtt cgcggtcctg
     4141 cgcgagctct ccgaggctcg gctgaagacc ctcgtcgaag gctatcgccc cggcgccgcg
     4201 ggcgacgagg gcaaggtcgc cgagctctac cgcggattcc tcgacgaggc cgcggcc
//
LOCUS       DASVQZ010000204         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_523985, whole genome shotgun sequence.
ACCESSION   DASVQZ010000204 DASVQZ010000000
VERSION     DASVQZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523985"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..942
                     /locus_tag="VF547_05610"
     CDS             247..942
                     /locus_tag="VF547_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8262333.1"
                     /translation="MIRVASYNMRKAIGTDRRRRPERTLEVLGELDADVIALQEADRR
                     FGTRASAIPLHMLEEHSDYKPVPFDGRDGSLGWHGNALLVRKGVEVLRKERLHLPSLE
                     PRGAVMADICLDGEELRVVGMHLDLSGLWRRQQAHAILHHLQTREAPMPAVLMGDLNE
                     WSVRGGCLRDFGHHLTFADCGRSFHVRRPVARLDRIMVTEGLAIEGCGVHQSPTARKA
                     SDHYPVWARLRRT"
     gene            complement(946..>2132)
                     /locus_tag="VF547_05615"
     CDS             complement(946..>2132)
                     /locus_tag="VF547_05615"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8262334.1"
                     /translation="NEQLASIEQRQQAAGSLVDYKRLDHRLQVLMRDQRMIGLAVGVV
                     ENGEIRFLKGYGETVAGSGEKVTTDTVFRWASLSKGVAGDMVALLADKGKLSLYEPIA
                     KYSTSLRLPAGNEHKATVSDLLSHRLGLFGHANDSKLEDGMDPRFLRASLYQLNSICP
                     PGSCWAYQNVAYDASSEIVEKVTGKSYAEAVREHLFGPLGMASASLSRDGLVSAPRWA
                     RPHVGGKNSKPVEVTESYYRVPAAGGVNSTIKDLAVWMQAQMGLEPNVLSPRVLEAVQ
                     TARVRTPGELGRMRKFRERVRTAAYGLGWRIYDYSGHRVVAHRGGVRGYRSLIMFDPA
                     LKSGVVALWNGSSGQPWGLEFEVMDMLYRLEPRDWLEVDGKPEPEASAAEGNNSSADS
                     AN"
BASE COUNT          331 a          737 c          741 g          323 t
ORIGIN      
        1 ggcggccatg ccgacctgcg gtgctcgatg gaggcgcgga tcaacggcat cgcgccggtc
       61 gccgcgatcg accagggcga tacggtcgac cgggccgtga tcggcgccgc caagaaggtc
      121 gtgcgcgcgc tcgaccacca gctcggcaag ctcaacgacc gcagccgcgg acgataggga
      181 cggttacttt ccgcgccggc cgcggttaag ggcgacccga ggctcgagaa agcaaccgtc
      241 cgctccatga tccgcgtcgc cagctacaac atgaggaagg cgatcggcac cgaccgccgg
      301 cgccggccgg agcggaccct cgaggtgctg ggcgagctcg acgccgacgt gatcgcgctg
      361 caggaggcgg accgccggtt cgggaccagg gccagcgcga tcccgctgca catgctcgag
      421 gagcatagcg actacaagcc ggtcccgttc gacgggcgcg acggcagcct gggctggcac
      481 ggcaacgccc tgctggtgcg caagggggtg gaggtgctcc gcaaggagcg gctccatctg
      541 ccctcgctcg agccgcgcgg cgcggtgatg gcggacatct gcctcgacgg cgaggagctg
      601 cgcgtggtcg gaatgcacct cgacctgtcc ggcctctggc ggcgccagca ggcgcacgcc
      661 atcctccacc atctgcagac ccgcgaagcg ccgatgccgg cggtgctgat gggcgacctc
      721 aacgaatgga gcgtccgcgg cggctgcctg cgcgacttcg gacatcatct caccttcgcc
      781 gattgcgggc gaagcttcca cgtccggcgg ccggtggcgc gcctcgaccg gatcatggtg
      841 accgagggcc tcgccatcga gggctgcgga gtccaccaga gcccgaccgc ccgcaaggcg
      901 agcgaccatt atccggtctg ggcccgtttg cggcggacct gatcgtcagt tcgccgagtc
      961 ggccgagctg ttgttgccct cggccgcgga ggcctcgggc tccggcttgc cgtcgacctc
     1021 gagccagtcg cggggctcca ggcggtagag catgtccatc acctcgaact cgagccccca
     1081 gggctgcccg ctcgatccgt tccagagcgc gacgacgccc gacttgagcg ccggatcgaa
     1141 catgatcagc gaccggtagc ccctgacccc tccgcgatgg gcgaccacgc ggtgacccga
     1201 ataatcgtag atccgccagc ccaggccata ggcggcggtt cgcacccgct cgcggaactt
     1261 gcgcatccgg ccgagctcgc cgggcgtgcg cacccgcgcc gtctgaacgg cctcgagcac
     1321 gcgcggcgac agcacgttcg gctcgaggcc catctgcgcc tgcatccaga cggcgagatc
     1381 cttgatcgtc gagttgaccc cgccggcggc ggggacccgg taataggatt cggtgacctc
     1441 gaccggcttc gaattcttgc cgccgacatg gggtcgggcc cagcgagggg ccgagaccag
     1501 cccgtcgcgg ctcaggctcg cgctggccat tcccagcggc ccgaacagat gctctcgcac
     1561 cgcctcggcg tagcttttgc cggtcacctt ctcgacgatc tcggacgagg cgtcgtaggc
     1621 gacgttctga taggcccagc agcttcccgg cgggcagatc gagttgagct ggtagagcga
     1681 ggctcggagg aagcgcgggt ccatgccgtc ctcgagcttc gaatcgttgg cgtggccgaa
     1741 cagcccgagc cggtgcgaga gaaggtcgga cacggtcgcc ttatgctcgt tgccggcggg
     1801 caggcgcagc gaggtcgaat atttggcgat cggctcgtag agcgacagct tgcccttgtc
     1861 ggcgagcagg gcgaccatgt cgcccgcaac gcccttcgac agggacgccc agcggaagac
     1921 ggtgtcggtg gtgaccttct cgccgctgcc ggcgacggtc tcgccatagc ctttgaggaa
     1981 gcggatctcg ccgttctcga cgactccgac ggcgagtccg atcatccgct ggtcgcgcat
     2041 cagcacctgc aggcggtggt cgagccgctt atagtcgacc aggcttccgg ccgcctgctg
     2101 gcgctgctcg atgctggcca gctgctcgtt gg
//
LOCUS       DASVQZ010000205         5916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_620839, whole genome shotgun sequence.
ACCESSION   DASVQZ010000205 DASVQZ010000000
VERSION     DASVQZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620839"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2777
                     /locus_tag="VF547_05620"
     CDS             <1..2777
                     /locus_tag="VF547_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262335.1"
                     /translation="IVVGDVADGGGDGVEFVSNGGGEHAVIETLENVLAVTTLAATGA
                     GPITYEIIGGPDAWFFSLDPNSGALAFLYEPDFEYRSDYGWDSVYDITVRAHNGTTYD
                     IQHLSIVVGNADEAPQFFSYWGSDTVDLTMTENGWQVGEVYASDPDGWQEQVTYSIAG
                     GADAGLFEVDPYSGALTFVFEQRPNFEAPGDADGDNVYEVVVAAVSGELGATQAFRVT
                     IGDDNEGVWITSDGGGSSASLSVAEGERMVTEVTAWDPDGTIPGFLIVGGADAARFTI
                     DAATGRLSFIKAPDHEIPGDADLDNVYEVTVAASDGYYQTRQDLSITVADADEPVEFV
                     SYSGSDIAYLWVDENGVAAARAEAADGDGGQVTYSISGGADMELFAIDPATGALSFIG
                     APDFEFPADSDGDNVYSVMVTASAGSSSDWQAYRLMVRNVNEEVVITSNGGGSSASVE
                     AGENQVAVTTVVAEDYDETFPTFAIVGGADAARFTIDAVSGLLQFVSAPDREAPGDAG
                     GDNVYDVIVRATDGSLFDDQSIAVTVVNVDEGLAITSYVGQDHVSLAMAENGTAVGQV
                     AAVDQDGDPVTYLIAGGADAALFRIDPSTGALSFAAAPNFEAPADSDGDNVYKVEVAA
                     VSGAFTDVQAFAVTVYNSNEPLVITSNGGGAGASVSVGENSRAVTTVAATDADGTAPT
                     YSIVGGADSARFTIDPQTGLLQFVTAPNHEAPTDADGDNVYRVVVQAGDGQFTDLQTL
                     DVRVLNLRDGATVTGTSGGDSISGTSTNPALRTTDSEDSVFGRDGHDTILGLGGDDDL
                     YGDGGNDVLTGGGGADRLTGGLGKDQFVYNLVSDSTPGGRDVIADFSRSQGDKISLSA
                     IDANTLVGGNQAFSFIGAAAFSGVAGQLRYSTSGGATFVSGDVNGDSVADFQIELSGI
                     LAPVASDFVL"
     gene            complement(2919..3365)
                     /locus_tag="VF547_05625"
     CDS             complement(2919..3365)
                     /locus_tag="VF547_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262336.1"
                     /translation="MSLALAAALLAQAPTAETPRDFVTRIYAGYRDPGYNPLDRPERL
                     FALPLVAAIREDARLSRDEVGRLDGDPLCQCQDHEGLEPLVEAVSRPAARTALADVRL
                     RFPGYGDRQVRLRLVRTARGWRIADVATPDSPSLLRELRRSNRRRR"
     gene            complement(3362..3766)
                     /locus_tag="VF547_05630"
     CDS             complement(3362..3766)
                     /locus_tag="VF547_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX8262337.1"
                     /translation="MARWLMKSEPGTYSWEALGRDGGTEWDGVRNPAARLHLRAMRPG
                     DEAFFYHSGGERAVVGIMRVVREGRPDPGDPDWVSVRVEPVRPLGPVTLKAIKADPRL
                     AGMELIRQSRLSVSPVRDEEWAAILELAGEAN"
     gene            3825..4337
                     /locus_tag="VF547_05635"
     CDS             3825..4337
                     /locus_tag="VF547_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262338.1"
                     /translation="MRRPLLLLGLLLASCGEPVTVANRAAPDNEAIEADAVAAPARPV
                     RIGELGPNFAACSAAGTTRNLKAGESLPVRWAPFEHAPESGSVAARSSFFICSRSLDQ
                     KWFGIVFDDGGTLAERCAVAEPVTRRRDYDGPCRSGWVQSAFVKVIAGGGPPQGGPAP
                     EAADSAAAGA"
     gene            4593..5252
                     /locus_tag="VF547_05640"
     CDS             4593..5252
                     /locus_tag="VF547_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8262339.1"
                     /translation="MNRIVRAAGFAAAVFALVAGASYSDPASAGETVTAASVAAGSAP
                     AASLMSDPNLFAKMWAGVTALVTPKAEAPVEAAPSQPGKLADLVLAYAADTTRDREQE
                     CLANAVYFEARSEPIEGQLAVAEVVLNRAASGRYPTDLCAVITQKAQFSFIRRGKFPR
                     ANRDSEAWKKAVAIADIAQKKLAGSLPSGVLWYHATYVSPSWGKRLTKQTQIGLHIFY
                     S"
     gene            complement(5391..>5916)
                     /locus_tag="VF547_05645"
     CDS             complement(5391..>5916)
                     /locus_tag="VF547_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX8262340.1"
                     /translation="AYVPDFLGPDEERALAGRIAGLPFKPFEFHGFTGNRRTVSFGLH
                     YAFDGSGLREAVPIPDWLQPLRERAAALAGRAPAEFVHALVIEYAAGAGIGWHRDRPV
                     FGDVVGISLLAPAPLRFRRRAGEKWDRFTLTAEPRSAYLLQGAARHEWEHSIPQLETL
                     RYSVTLRTLREGKR"
BASE COUNT          901 a         2119 c         2019 g          877 t
ORIGIN      
        1 agatcgtcgt cggcgacgtc gcggacggcg gcggcgacgg agtcgagttc gtttcgaacg
       61 gcggcggcga gcatgccgtc atcgagacgc tcgagaacgt cctcgcggtg accaccctgg
      121 ccgccaccgg cgccggcccg atcacctacg agatcatcgg cggccccgat gcctggttct
      181 tcagcctcga tcccaacagc ggcgcgctcg ccttcctcta cgagcccgat ttcgagtatc
      241 gcagcgacta cgggtgggac agcgtctacg acatcaccgt ccgcgcccac aacggaacga
      301 cgtacgacat ccagcatctg tcgatcgtcg tcggcaatgc cgacgaagcg ccccaattct
      361 tttcctactg gggctccgac acggtcgacc tgaccatgac cgagaacggc tggcaggtcg
      421 gcgaggtcta tgcctccgac ccggacggct ggcaggagca ggtgacctat tcgatcgccg
      481 gaggcgccga cgccggcctc ttcgaggtcg atccgtacag cggcgcgctc accttcgtct
      541 tcgagcagcg gccgaacttc gaggcgccgg gcgatgccga cggcgacaat gtctatgagg
      601 tcgtcgtcgc cgccgtttcc ggcgagctcg gcgccacgca ggccttccgg gtgacgatcg
      661 gcgacgacaa tgaaggcgtg tggatcacgt cggacggcgg cggctccagc gcctcgctct
      721 cggtcgccga gggcgagcgc atggtcaccg aggtcaccgc ctgggatccg gacggaacca
      781 tccccggctt cctgatcgtc ggcggcgccg acgcggcccg attcaccatc gatgccgcga
      841 ccggacggct gagcttcatc aaggcgccgg accatgagat cccgggcgat gccgacctcg
      901 acaacgtcta cgaggtcacg gtcgccgcca gcgacggcta ttaccagacc cgccaggatc
      961 tttcgatcac cgtcgccgac gccgacgagc cggtcgagtt cgtctcctat tccggctcgg
     1021 acatcgccta tctgtgggtc gacgagaacg gcgtcgccgc ggcccgggcc gaggcggccg
     1081 acggcgacgg cggccaggtg acctattcga tcagcggcgg cgccgacatg gagctgttcg
     1141 cgatcgaccc ggccaccggc gccctcagct tcatcggagc gcccgacttc gagttcccgg
     1201 cggactccga cggcgacaat gtctattcgg tgatggtcac ggccagcgcc ggctcgtcga
     1261 gcgactggca ggcctatcgg ctgatggtcc gcaacgtcaa cgaggaggtg gtgatcacct
     1321 cgaacggcgg cggctcgagc gcctcggtcg aggccggtga gaaccaggtc gccgtcacga
     1381 ccgtggtggc ggaggattat gacgagacct tcccgacctt cgccatcgtc ggcggcgccg
     1441 acgccgcgcg cttcacgatc gacgcggtca gcggattgct gcaattcgtc tcggcgcccg
     1501 accgcgaggc gcccggcgat gccggcggcg acaatgtcta cgacgtgatc gtccgggcga
     1561 ccgacggcag cctgttcgac gaccagtcga tcgcggtcac ggtcgtcaat gtcgacgagg
     1621 ggctcgccat cacttcctat gtcggccagg accatgtctc cctggcgatg gccgagaacg
     1681 gcaccgcggt cggccaggtc gccgccgtgg accaggacgg cgatccggtg acctatctga
     1741 tcgccggcgg cgccgacgcc gccctgttcc ggatcgaccc atctaccggg gcgctgagct
     1801 tcgccgccgc gcccaatttc gaggcgccgg cggattcgga cggcgacaat gtctacaagg
     1861 tcgaagtcgc ggcggtctcc ggcgcgttca ccgacgtcca ggccttcgcc gtcaccgtct
     1921 acaattcgaa cgagccgctg gtcatcacct cgaacggcgg cggcgccggc gcctcggtct
     1981 cggtcgggga gaacagccgc gccgtcacca cggtcgccgc gaccgatgcg gacgggacgg
     2041 cgccgaccta ttcgatcgtc ggcggcgccg attcggcccg attcaccatc gatccgcaga
     2101 cgggcctgct gcagttcgtc accgcgccga accatgaggc tcccaccgat gccgacggcg
     2161 acaatgtcta cagagtcgtc gtccaggccg gcgacggcca gttcaccgac ctccagactc
     2221 tcgacgtccg ggtgctcaac cttcgcgacg gcgccaccgt gacgggcacg agcggcggcg
     2281 actcgatctc cgggacgagc accaacccgg ccctgcggac caccgactcg gaggattccg
     2341 tgttcggccg cgacggccac gacaccatcc tcggcctcgg cggcgacgac gatctctacg
     2401 gcgacggcgg caacgacgtg ctgaccggcg gcggcggggc cgaccggctg accggcgggc
     2461 tgggcaagga ccagttcgtc tacaatctgg tcagcgactc gacgcccggc ggccgcgacg
     2521 tcatcgccga cttcagccgc tcgcaggggg acaagatctc gctgagcgcg atcgacgcca
     2581 acacgctcgt cggcggcaac caggccttca gcttcatcgg cgcggccgcc ttctcgggcg
     2641 tcgcggggca gctgcgctac tcgacctcgg gcggggcgac gttcgtctcg ggcgatgtga
     2701 acggcgattc cgtcgccgac ttccagatcg agctgagcgg gatcctggcg ccggtcgcct
     2761 cggacttcgt gctgtagcgc ccattcctcc ccggcctttg ccggggaggg ggaccgtccg
     2821 aaggacggcg gaggggttct cgcgggctcc gaaagaaccc ctccaccggg cttcgcccgg
     2881 tccccctccg ccggggaatc cgggggagga attgaccgtt aacgcctccg ccggttggag
     2941 cgccgcagct cgcgcagcag gctcgggctg tcgggcgtgg cgacgtcggc gatccgccag
     3001 ccccgcgccg tgcgcaccag ccgaagccgg acctggcggt cgccatagcc ggggaagcgg
     3061 aggcggacat ccgccagggc ggtcctcgcc gccggccggc tcacggcctc gacgagcggc
     3121 tcgagccctt catggtcctg gcactggcag agcgggtcgc cgtcgagccg gccgacctcg
     3181 tcccgcgaga gccgggcatc ctcgcggatc gcggccacca gcggcagggc gaagaggcgc
     3241 tcaggccggt cgagcggatt gtagccggga tcgcgatagc cggcatagat ccgggtcacg
     3301 aagtcccggg gcgtttccgc cgtcggggcc tgggccagca aggcggcggc aagggcgagg
     3361 ctcaattcgc ctctccggcc agctcgagga tcgccgccca ttcctcgtcg cgaaccggcg
     3421 agaccgagag ccgggactgg cggatcagct ccatgccggc gagccggggg tccgccttga
     3481 tcgccttcag ggtcaccggg ccgagcgggc gcaccggctc gacccggacg gagacccagt
     3541 cgggatcgcc cgggtcgggc cgtccttccc ggacgacgcg catgattccg accaccgccc
     3601 gctcgccgcc gctgtggtag aagaaggcct cgtcgccggg gcgcatcgcc ctgaggtgga
     3661 ggcgggcggc cgggttgcgg accccgtccc attcggtgcc gccgtcgcgg ccgagcgcct
     3721 cccagctgta ggtgccgggt tcggatttca tcagccagcg ggccatccct gcctccgttc
     3781 gcggccagcg tagatttttc cggcgcggcg cgccacaagg agccatgcgg cgcccgctcc
     3841 ttctcctcgg cctcctcctg gcctcgtgcg gcgagccggt gacggtcgcc aaccgcgcgg
     3901 cgccggacaa tgaggcgatc gaagccgatg ccgtcgccgc cccggcacgc cccgtccgga
     3961 tcggcgagct cggaccgaat ttcgccgcct gcagcgccgc cgggacgacc cgcaacctga
     4021 aggcgggcga atcgctcccg gtgcgctggg cgccgttcga gcacgcgccc gagagcggca
     4081 gcgtcgcggc gcgctcgagc ttcttcatct gcagccgcag cctcgaccag aaatggttcg
     4141 ggatcgtgtt cgacgacggc ggaacgctcg ccgagcggtg cgccgtcgcc gagccggtga
     4201 cccgccgccg cgactatgac ggaccctgcc ggtccggctg ggtacagagc gctttcgtca
     4261 aggtgatcgc cggcggcggg ccgccgcagg gcggccccgc ccccgaggcc gccgattccg
     4321 cggctgcagg tgcttgaaaa aagggccttt ttttaaaagt tccggccaaa ggcaaatatt
     4381 ttttcagcgt gtctccggag tcacacggct catcgcttcg gcggggtcga cgggttgatt
     4441 agcggccccg cttcgaccat ctgcccctca gcttagtagc gagagcgggt ccgccggagc
     4501 ctttcaggca gcggacgctc gagacggggg gcgatcaggg gtgcggttct cgcatccggg
     4561 ggatcagccg gccgcataaa aaaagagagt gaatgaaccg gatcgttcgc gccgcgggct
     4621 tcgccgcggc tgtctttgcg ctcgtcgcag gagccagcta ctccgacccc gccagcgccg
     4681 gcgagaccgt gaccgcagcg tccgtggccg ccggctcggc gcccgccgcg tcgctcatgt
     4741 cggaccccaa tctgttcgcc aagatgtggg cgggagtgac cgccttggtc actccgaagg
     4801 cggaggcgcc ggtcgaggcc gcgccgtcgc agccgggcaa gctggccgac ctggtcctcg
     4861 cctatgccgc cgacacgacc cgcgaccgcg agcaggaatg cctcgccaat gcggtttatt
     4921 tcgaggcccg cagcgaaccg atcgagggac agctcgccgt cgcggaggtc gtcctcaacc
     4981 gcgccgcctc gggccgctac ccgaccgatc tctgcgccgt catcacccag aaggcgcaat
     5041 tctcgttcat ccgccgcggc aagttcccgc gcgccaaccg cgattccgag gcgtggaaga
     5101 aagcggtcgc catcgccgac atcgcccaga agaagctcgc cggctcgctc ccgtccggag
     5161 tgctctggta ccacgccacc tacgtctccc cgagctgggg caagcgcctc accaagcaga
     5221 cccagatcgg cctgcacatc ttctacagct gatcccggtc cttcgatacg ggccttcgac
     5281 ttcgctcagt ccctgctcag gatgagggct tgggaagcct ccctgccgct cagcccgagt
     5341 agccgctgag cgaagtcgaa gcggcgcccc gaaggacgtg ccgaagggcg tcatcgcttt
     5401 ccctccctca aggtcctcaa cgtcaccgag tagcgcagcg tctccagctg ggggatgctg
     5461 tgctcccact cgtgccgggc cgcgccctgc agcaggtagg cggagcgggg ctcggccgtc
     5521 agcgtgaagc ggtcccactt ctcgcccgcc cggcggcgga agcgcagcgg cgcgggcgcg
     5581 agcagcgaga ttccgacgac gtcgccgaac accggccggt cgcggtgcca gccgatgccg
     5641 gcgccggcgg catattcgat caccagcgca tggacgaact ccgccggcgc gcgcccggcg
     5701 agggccgcgg cgcgctcgcg cagcggctgc agccagtccg ggatcggcac ggcctccctc
     5761 agcccgctgc cgtcgaaggc gtaatggagg ccgaacgaga cggtgcggcg gttgccggtg
     5821 aagccgtgga actcgaatgg cttgaacggc aggccggcga tccgcccggc gagcgccctt
     5881 tcctcgtccg ggccgaggaa atccggcacg taggcc
//
LOCUS       DASVQZ010000206         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_429628, whole genome shotgun sequence.
ACCESSION   DASVQZ010000206 DASVQZ010000000
VERSION     DASVQZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429628"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF547_05650"
     CDS             <1..508
                     /locus_tag="VF547_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262341.1"
                     /translation="HQGCSGCGEDFELELTAEQVGLAPRPEDEQLPEAPAAGVRYRRR
                     DFVVRAVCVADMIAAEAAGGVEAARELLAERTAPQAPAAARARVAEMLEALDPGADTT
                     IEVPCPHCGREQELRLDPAAFVWEELAARAPRILRDVADLARAYHWSERDILAMPAAR
                     RAFYLAAA"
     gene            1366..1686
                     /locus_tag="VF547_05655"
     CDS             1366..1686
                     /locus_tag="VF547_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262342.1"
                     /translation="MAEPAAGSADRIAPAPDGLARPAPSATGPRTAAAEPQPRPERAA
                     PGAVAAAVRLPPRPAELQSAPAPVEIRIGRVDIHAAPPAAAPAAAVSAPGPSLDAFLA
                     RPRR"
     gene            1683..2930
                     /locus_tag="VF547_05660"
     CDS             1683..2930
                     /locus_tag="VF547_05660"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX8262343.1"
                     /translation="MSDHRAIAAVTATLKAIIQEAASLAVGQSEVRLGAPVAKLNDGT
                     APTVNLFLFRTTPNAAMRNSHLPTRRSDGSARARAQTAVDLHYILTFYGDPAQFVPER
                     LLGAVTLALEERPILTPAMIESALAAAADTLPLLKQADLPDAPSRVRIAPATVTLEEF
                     TKIWSIFFQVPYGLSVAYVCSHVVIESDERFATVLPVIRPNVLAGPISGFALDWAGPD
                     SGGPGPVAAGGGLFLAGKGLGRSGTTVDIDGAETPFDSQSGSGIRLTLPANLKVGIHL
                     AQAVAPPTSAATPPHLRRRSNTIPFALHPSISVGAVAHDDAEPRTGTLEVGFSPPVED
                     GQRVTVTLDARALDGPLGIVLDPQAPGAFPAAQLSFPFEDVPAGLYLVRAHVDGFASL
                     PELGTDASSSDFGLIVGPLADLT"
     gene            2927..>4834
                     /locus_tag="VF547_05665"
     CDS             2927..>4834
                     /locus_tag="VF547_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8262344.1"
                     /translation="MSGTTAPGPLSDAFPAVRSWLSDEAPIETVPPTRADLLAAMFGL
                     DDFARNTVLLAAFCALEPEGAQLIAGLQGGGGRGAATVALALARVPGAHWSAFAAQAP
                     LRAWALIPAASGDSLTSGPLDLPESVLFYLLGAAALSEELALSSRRLEPPADLSPARR
                     RLADRIAALIGSGRGEILQLCGADPLGKEQAAAAGALAAGKGLYALNALMLPPATSDI
                     ARLAQMWRRDLRLLESALLVDAAGVAETRPVGLFAEMLRTPFLVAAPDAVPLGNLQSL
                     RLDMPRATAAEQVPIWERQLGPLAKKLNGSVGRLAGHFNVSPELAGSVAAELERAVSE
                     AKGPSGMGKEPAAEDLGKIAWEACRQFARPRMDDLARRVEGRAEWKDLILPEAQLRTL
                     RAMAAQVRHRATVYESWGFGARSMGRGLGISALFAGPSGAGKTLAGEVLGAELELDVY
                     RIDLSAIVSKWIGETEKNLRRVFDAAEEGCAILQFDEADALFGKRSEVKDSHDRHANI
                     EVSYLLQRLEEYRGLSILTTNLKNNIDPAFLRRIRFIVDFGFPEPAERVAIWRGIFPK
                     ETPLGDLDFERLGQLNLAGGSIRNVAVAAAFLAAGDGSKRQRVEMSHILAAARTEYEK
                     SGRPMTGAELRG"
BASE COUNT          583 a         1907 c         1709 g          635 t
ORIGIN      
        1 ccaccagggc tgctcgggct gcggcgagga tttcgagctc gagctcaccg ccgagcaggt
       61 cggcctcgcc ccgcggccgg aggacgagca gctgcccgag gcgccggccg ccggcgtccg
      121 ttaccgccgc cgcgatttcg tcgtgcgcgc ggtctgcgtc gccgacatga tcgccgccga
      181 ggccgccggg ggagtcgagg ccgcgcgcga gctgctcgcc gagcgcaccg ctccgcaagc
      241 cccggcggcg gcgcgggcgc gcgtggcgga gatgctcgag gcgctggacc cgggcgccga
      301 cacgacgatc gaggtgccgt gcccccattg cgggcgggag caggagcttc ggctggatcc
      361 ggccgccttc gtctgggagg agctcgccgc gcgcgcgccg cggatcctgc gcgacgtcgc
      421 cgacctggcc cgtgcctatc actggtcgga acgcgacatc ctcgccatgc cggcggcccg
      481 gcgcgctttc tacctggcgg ccgcatgagc gacttcctgg accgactggc ctcgcggtcc
      541 cagggcggct cgccggtcct gcatccgcgt ccgctctcgc tgttcgaggg cgggccggag
      601 ctcctcgagg aagtccatct cgagcgcgaa gcggatcctg cgcggcctcc gccggccgat
      661 gggcccgcgc gatcggcggc cggcttcgca cagcgtgccg ccgcgcccgc acgggcggcg
      721 gccgagccgg cacgagccga gatcgcgctc ccgggagcgg cggccgggac cgcgcccggc
      781 gaattcccac gcgcggcgtc tgagcccgga cccgtcggat ccgtgctgca acgaggagcg
      841 gccgagccgg cgcccgcaga gaccgcccgg gcacgggcag cggccgcgcc ggcacgagcg
      901 gcgaccgccg gcgctccggc ggaaacggcg cccgcttggg tcgtgggcgc gcccgcgagg
      961 acgccccagc ccgttcgtcc gggtgcggcc cggcccgacc cgcctctgcc ggaacggcat
     1021 ccggcttcgc cggtccgaac cgaagcctct gcggcggcat cgactctccg gccggattcg
     1081 ccggaagccg ccgtcggccg tccgtcaccg gctccggccg gcgctcgggg cgcggcggtt
     1141 cccgcgccgg ggccgcagtc ggccgccccg ctcctgcgtc ggcgaaccgc ggacggggcg
     1201 gccgggccgc ccgacgaggc ccggtccctg ccgaaggcgc cggcgctcgg gcccgaggcg
     1261 ttcgaattcg cgccggatcc cgccggcaag gccggccctc cagtctcgcg cgccgccggg
     1321 cgcgccgctt cggcggcccg gccctccgcg ttccgacccg agagcgtggc ggagcctgcc
     1381 gccggatccg ccgaccggat agcgccggcc ccggacggcc tggcgcggcc ggcgcccagc
     1441 gcgaccgggc ctcggacggc ggccgccgag ccccagcctc gtccggaaag agccgcgccc
     1501 ggcgccgtcg cggctgcggt ccgcctgcct ccgcggcccg ccgagcttca gtccgcccct
     1561 gcgccggtcg agatcaggat cggtcgggtc gacatccatg cggcgccgcc cgccgccgcg
     1621 cccgccgccg ccgtttccgc tccgggaccc tcgctggacg cctttctcgc ccggccacgg
     1681 cgatgagcga ccatcgcgcc atcgccgcgg tcaccgccac tttgaaggcg atcatccagg
     1741 aagccgccag cctcgcggtg ggccagagcg aagttcggct cggagccccc gtcgccaagc
     1801 tcaacgacgg caccgcgccg acggtcaatc tgttcctgtt ccgcaccacg cccaacgccg
     1861 cgatgcgcaa ttcccacctc cccacccgcc gctccgacgg cagcgcgcgg gcgagggcgc
     1921 agacggcggt cgacctccac tacatcctca ccttctacgg agaccccgcc cagttcgtgc
     1981 ccgagcgcct gctcggagcc gtcaccctgg cgctggagga gcggccgatc ctcaccccgg
     2041 cgatgatcga gagcgctctc gccgcggcgg ccgacaccct ccccttgctc aagcaggccg
     2101 acctgcccga cgccccgtcc cgggtccgga tcgcgcccgc caccgtcacc ctcgaggaat
     2161 tcaccaagat ctggtcgatc ttcttccagg tcccctacgg actctcggtc gcctatgtgt
     2221 gcagccacgt cgtcatcgag agcgacgagc gcttcgccac cgtgcttccg gtgatccgcc
     2281 ccaacgtcct ggccgggccg atctcgggct tcgcgctcga ctgggcggga cccgattcgg
     2341 gcgggcccgg gccggtggcc gcgggcggcg gcctgttcct cgccggcaag gggctcgggc
     2401 gaagcgggac gacggtcgac atcgacgggg ccgagacgcc gttcgattcg cagtcgggca
     2461 gcggcatccg actgaccctg ccggcgaacc tcaaggtcgg aatccatctc gcccaggcgg
     2521 tggcgccgcc gacctcggcg gcgactccgc cccacctgcg ccggcgctcg aacacgatcc
     2581 cgttcgcgct ccatccctcg atctcggtgg gcgccgtcgc ccacgacgac gccgagccgc
     2641 gcaccggcac gctcgaggtg ggtttctcgc cccccgtcga ggacggccag cgggtgaccg
     2701 tgaccctcga cgcccgagcc ctcgacgggc cgctcgggat cgtgctcgac ccgcaggcgc
     2761 cgggcgcctt tccggccgct cagctcagct tcccgttcga ggacgtgccg gcgggcctgt
     2821 atctggtgcg ggcccatgtc gacggcttcg ccagcctgcc ggagctcggc accgatgcct
     2881 cctcgtccga tttcggcctg atcgtcggcc cgctggcgga cctcacatga gcggcacgac
     2941 ggcgcccggc ccgctcagcg acgctttccc ggcggttcgg tcgtggctca gcgacgaggc
     3001 cccgatcgag acggtgcctc cgacccgcgc cgacctgctg gcggcgatgt tcggcctcga
     3061 cgacttcgcc cgcaacaccg tcctgctcgc cgccttttgc gcgctcgagc ccgagggggc
     3121 gcagctgatc gccgggctgc agggcggcgg cgggcgcggc gctgcgacgg tcgccttggc
     3181 gctggcccga gtgcccggcg cgcactggtc cgctttcgcc gcccaggcgc cgctgcgcgc
     3241 ctgggcgctg atccccgccg cctcgggcga ctcgctgacc tccggcccgc tcgacctgcc
     3301 cgaatcggtg cttttctacc tgctcggcgc ggccgcgctg agcgaggagc tcgccttgtc
     3361 gtcgcggcgg cttgagccgc ctgccgacct ctcgcccgcg cgccggcgcc tggccgaccg
     3421 gatcgcggcg ctgatcggct cgggccgcgg cgagatcctc cagctctgcg gcgccgatcc
     3481 gctcggcaag gagcaggcgg cggcggccgg agcgctcgcg gccggaaagg gcctctacgc
     3541 gctcaacgcg ctgatgctgc cgccggcgac gagcgacatc gcccggctgg cgcagatgtg
     3601 gcggcgcgac ctgcggctgc tcgagtcggc gctgctggtg gatgccgccg gggtcgccga
     3661 gacccgcccg gtcgggctgt tcgcggagat gcttcggact ccgttcctgg tcgccgcccc
     3721 cgacgcggtc ccgctcggca acctccagtc gctgaggctc gacatgccgc gcgcgaccgc
     3781 cgccgagcag gttccgatct gggagcggca gctgggaccg ctggccaaga agctcaacgg
     3841 ctcggtcggc cgcctcgccg gccatttcaa cgtctccccc gagctcgccg gaagcgtcgc
     3901 ggccgagctc gagcgcgcgg tcagcgaggc caagggcccg tcgggcatgg gcaaggagcc
     3961 ggccgcggag gatctcggca agatcgcctg ggaggcctgc cggcagttcg cccggccgcg
     4021 catggacgac ctcgcccggc gggtcgaggg ccgcgccgaa tggaaggacc tgatcctgcc
     4081 cgaggcccag ctgcgcaccc tgcgcgcgat ggcggcgcag gtccggcatc gcgccaccgt
     4141 ctacgaaagc tggggcttcg gcgcgcgctc gatgggccga gggctcggca tcagcgccct
     4201 gttcgccggc ccgtccgggg ccggcaagac gctcgccggc gaggtgctcg gcgccgagct
     4261 cgagctcgac gtctaccgga tcgacctgtc ggcgatcgtc tcgaagtgga tcggcgagac
     4321 cgagaagaac ctgcgccggg tgttcgacgc cgccgaggag ggctgcgcca tcctccagtt
     4381 cgacgaggcc gacgccctgt tcggcaagcg ctccgaggtc aaggacagcc acgaccgcca
     4441 cgccaatatc gaggtcagct acctgctgca gcggctcgag gaatatcgcg gcctctcgat
     4501 cctgaccacc aacctcaaga acaacatcga tccggccttc cttcgccgga tccgcttcat
     4561 cgtcgacttc ggtttccccg agccggccga gcgggtcgcg atctggagag gcatattccc
     4621 caaggagacc ccgctcggcg acctcgactt cgagcggctc ggccagctca acctcgccgg
     4681 cggctcgatc cgcaacgtcg ccgtcgccgc cgccttcctc gccgccgggg acggatcgaa
     4741 gcggcagcgg gtggagatga gccacatcct cgccgccgcc cgcaccgaat atgagaagtc
     4801 cggccggccg atgaccggcg ccgagctgcg cgga
//
LOCUS       DASVQZ010000207         4447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_144579, whole genome shotgun sequence.
ACCESSION   DASVQZ010000207 DASVQZ010000000
VERSION     DASVQZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144579"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VF547_05670"
     CDS             <1..140
                     /locus_tag="VF547_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8262345.1"
                     /translation="VERFFCKLKQFRRVATRFDKLARNFLAAVLLASTRLWLRTYEST
                     P"
     gene            complement(145..1110)
                     /gene="mltG"
                     /locus_tag="VF547_05675"
     CDS             complement(145..1110)
                     /gene="mltG"
                     /locus_tag="VF547_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX8262346.1"
                     /translation="MKKLLALGLLAAAALAVGLWFIQGWSGAGPAAKATTVVIAPGTS
                     LIEAADQLEKAGVIASSERFLFQAKLFGGSEPIKAGEYAFPARASHSQTLALMQHGKT
                     LQRMVTIPEGLPSVLVHERLMGASGLTGEVPVPAEGSVLPNSYSYQRGEPRAAVLSRM
                     QAAMDRTLTELWPKRTPASVVKDRHEAVTLASIVEKETGKASERRMIAGVYSNRLRQG
                     MKLDADPTVIYPITKGRPLGRRIRQSELQANNGYNTYAMAGLPAGPIANPGRESIAAV
                     LDPAPTQALYFVADGTGGHVFANTYAEHQANVDKWFAIRRARGEM"
     gene            complement(1118..2377)
                     /gene="fabF"
                     /locus_tag="VF547_05680"
     CDS             complement(1118..2377)
                     /gene="fabF"
                     /locus_tag="VF547_05680"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX8262347.1"
                     /translation="MRRVVVTGLGMVSPLGGDIETSWKNILASKSGATRITRFDPTDY
                     ACQIACEVKPPEHEYGFDANKRVDHKVQRQVDPFIVYGLDAAGQAIEDAGLTDMSEEE
                     RFRAGVSIGSGIGGLPGIEKESIVLHERGPRRVSPHFVHGRLINLISGQVSIKYGLMG
                     PNHAVVTACSTGAHSIGDAARMIAMDDADVMLAGGAEDALCPLGIAGFAQARALSTNF
                     NDQPERASRPYDKDRDGFVMGEGAGVVVLEEYERARKRGAKIYAEMVGYGMSGDAYHV
                     TAPHPEGAGGYRAMEAAMKRSGLDLSDIDYINAHGTSTPLGDELELGAVRRLFGNNIA
                     RLSMSSTKSAIGHLLGGAGAVEAIFCILAMRDQIVPPTLNLDNPSDGTAGVDLVPHVA
                     KERKVKAVLNNSFGFGGTNASLVMKAV"
     gene            complement(2580..2816)
                     /locus_tag="VF547_05685"
     CDS             complement(2580..2816)
                     /locus_tag="VF547_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8262348.1"
                     /translation="MSETADRVKKIVVEHLGVEAEKVTEEASFIDDLGADSLDIVELV
                     MAFEEEFGVEIPDDAAEKISTVKDAIDYIEAHKA"
     gene            complement(2914..3654)
                     /gene="fabG"
                     /locus_tag="VF547_05690"
     CDS             complement(2914..3654)
                     /gene="fabG"
                     /locus_tag="VF547_05690"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX8262349.1"
                     /translation="MFDLSGMTALVTGASGGIGSAIAQALAGQGARLAVSGSNVEKLE
                     AFRARLGGDHVALACNLSDSAAVDALVPQAVEALGGKLDILVNNAGVTRDNLAMRMKD
                     EEWSDVIRINLEAAFRLARAALKPMMRARHGRIISITSVVGATGNPGQANYAASKAGL
                     VGMSKALAQEVASRNITVNCIAPGFIASAMTDALPEAQKEALLGRIPAGRLGEGNDVA
                     AAAVYLASREAGYVTGQTLHVNGGMAML"
     gene            complement(3750..>4447)
                     /locus_tag="VF547_05695"
     CDS             complement(3750..>4447)
                     /locus_tag="VF547_05695"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8262350.1"
                     /translation="GLSIDKADFVAGHSLGEYSALCAAGAFDLPTTARLLKLRGQAMQ
                     RAVPVGEGAMAALLGASVEIAQAVADAAAHGEVCVVANDNDPSQVVISGHRGAVERAL
                     EIAREKGAKRAVLLPVSAPFHSPLMEPAAREMEQALAEVEIEEPAVPLYANVTAAPVE
                     NPGEIRRLLVEQVTGMVRWRESVAAMAEAGVDHFVEFGGKVLGPMVKRIAPDAKATSV
                     VTMDDIDALVKEI"
BASE COUNT          651 a         1532 c         1498 g          766 t
ORIGIN      
        1 tggtcgagcg gttcttctgc aagctcaagc agttccgacg cgttgccacc cgcttcgaca
       61 agctcgcccg caacttcctc gctgcagtct tgctcgcctc aacccgactt tggctcagaa
      121 cttatgagtc cacgccctag gccgctacat ctcgccgcgg gcgcggcgga tggcgaacca
      181 tttgtcgaca ttcgcctggt gctcggcata ggtgttggcg aagacatgcc cgccggtgcc
      241 gtccgcgacg aagtagagcg cctgggtcgg cgctgggtcg agcaccgccg cgatcgattc
      301 cctgccggga ttggcgatcg gtcccgccgg caggccggcc atggcatagg tgttgtagcc
      361 gttattggcc tgcagctccg actgccggat ccggcggccc agcggccggc ccttggtgat
      421 cgggtagatg acggtcgggt cggcatcgag cttcatcccc tgccgaagcc ggttcgaata
      481 gaccccggcg atcatccgcc gttccgaggc cttgccggtc tccttctcga cgatcgaggc
      541 gagggtgacc gcctcgtggc ggtccttcac caccgacgcg ggcgtgcgct tcggccagag
      601 ctcggtcagg gtccggtcca tggcggcctg catccgactc agcacggcgg cgcgaggctc
      661 gccgcgctgg tagctgtagc tgttgggaag caccgagcct tccgccggca ccggcacctc
      721 gccggtgagc cccgacgccc ccatcagccg ctcgtggacg aggaccgaag gaaggccttc
      781 gggaatggtc accatccgct gcagagtctt gccgtgctgc atcagggcga gggtctgcga
      841 atggctcgcc cgcgcgggga acgcatactc gcccgccttg atcggctcgg agcctccgaa
      901 cagcttggcc tggaagagga accgctcgga ggaagcgatc acgccggcct tctcgagctg
      961 gtcggcggcc tcgatcaggc tggtgccggg cgcgatgacg acggtcgtcg ccttcgcggc
     1021 gggtccggct ccgctccagc cctggatgaa ccagagcccg acggcgaggg cggccgcggc
     1081 cagcaggccg agcgcgagca gcttcttcat ggtccggtca gacggccttc atcaccaggc
     1141 tcgcattggt gccgccgaag ccgaagctgt tgttgagcac cgccttgacc ttgcgctcct
     1201 tggccacgtg cgggacgaga tccacgcccg ccgtgccgtc gctcggattg tcgaggttga
     1261 gcgtcggcgg gacgatctgg tccctcatgg cgaggatgca gaagatcgcc tcgacggcgc
     1321 cggcgccgcc gagcagatgg ccgatcgccg acttggtcga gctcatcgag aggcgggcga
     1381 tgttgttgcc gaacaggcgg cggacggcgc cgagctcgag ctcgtcgccg agcggcgtcg
     1441 aggtgccgtg agcgttgatg tagtcgatgt cggagaggtc cagcccggag cgcttcatcg
     1501 ccgcttccat cgcccggtag ccgcccgcgc cttccgggtg cggggcggtg acgtgatagg
     1561 cgtcgcccga cattccgtag ccgaccatct cggcgtagat cttcgcgccg cgcttcctgg
     1621 cgcgctcata ttcctcgagc acgacgactc cggcgccctc gcccatgacg aagccgtcgc
     1681 gatccttgtc gtaggggcgg ctggcccgct cgggctggtc gttgaagttg gtcgagagcg
     1741 cccgcgcctg ggcgaagccg gcgattccga gcgggcagag cgcgtcctcc gcgcctccgg
     1801 cgagcatgac gtcggcatcg tccatcgcga tcatccgggc cgcgtcgccg atcgaatggg
     1861 cgccggtcga gcaggcggtg acgaccgcgt ggttcgggcc catcaggccg tatttgatcg
     1921 agacctgccc ggagatgaga ttgatcagcc ggccgtggac gaagtgcggc gagacccggc
     1981 gcgggccccg ctcgtggagg acgatcgact ccttctcgat gccgggcagg ccgccgatgc
     2041 ccgagccgat cgagactccg gccctgaaac gctcctcctc gctcatgtcg gtgaggccgg
     2101 catcctcgat cgcctggccg gccgcgtcca ggccgtagac gatgaacggg tcgacctggc
     2161 gctggacctt gtgatcgacg cgcttgttcg cgtcgaagcc atattcatgc tcgggaggct
     2221 tgacctcgca ggcgatctgg caggcataat cggtgggatc gaagcgcgtg atgcgggtgg
     2281 cgccgctctt cgaggcgagg atgttcttcc agctggtctc gatatcgccg cccagcggcg
     2341 acaccatccc gagaccggtc acgactacgc ggcgcatgcc ttaacctcca acaacaaaca
     2401 ggcccagtcc cgttcgggct gagcctgtcc ttcgatacgc gctccggatc aagtccgggg
     2461 tcagccgcta cccaggatga gcggttctcg ccgaaccgtc cgctcatgct gagtaggcat
     2521 tgagcgaagt cgaaatgccg tatcgaagca cagctcagag cgaacgttcg ggcgcctcct
     2581 caggccttgt gggcctcgat ataatcgatg gcgtccttga ccgtgctgat cttctcggcc
     2641 gcgtcgtcgg ggatctcaac gccgaactcc tcctcgaagg ccatgaccag ctcgacgatg
     2701 tcgaggctgt cggcgccgag atcgtcgatg aagctcgctt cctcggtgac cttctcggct
     2761 tcgacgccga gatgctcgac gacgatcttc ttcacgcggt cggcggtctc gctcatattc
     2821 gtgcccttcc ctggccgtag ttttgcgatg ccctagccgc tccctgccgg ggctggcaag
     2881 tggcggcatc ccaaacggat cattccccgg gcgttacagc atcgccatgc cgccgttgac
     2941 gtggagggtc tgtcccgtga cgtagccggc ctcccggctg gcgaggtaga cggcggccgc
     3001 cgccacgtcg ttcccctcgc cgaggcggcc cgcggggatg cggccgagca gagcctcctt
     3061 ctgcgcttcc gggagggcgt cggtcatcgc cgaggcgatg aagccggggg cgatgcaatt
     3121 gaccgtgatg ttgcggctgg cgacctcctg cgccagcgcc ttcgacatgc ccaccagccc
     3181 cgccttggag gcggcgtaat tggcctggcc gggattgccg gtggctccga ccaccgaggt
     3241 gatcgagatg atccggccgt gccgcgcccg catcatcggc ttcagcgccg ctcgggcgag
     3301 ccggaaggcg gcctcgaggt tgatgcggat gacgtcgctc cattcctcgt ccttcatccg
     3361 catggcgagg ttgtcgcggg tgacccccgc attgttgacc aggatgtcga gcttgccgcc
     3421 cagcgcctcg accgcctgcg gaacgagcgc gtcgacggcg gcggagtcgg agaggttgca
     3481 cgccagcgcg acatgatccc cgccaagtct ggcgcggaag gcctccagct tctcgacgtt
     3541 cgacccggag acggcaagcc gagccccctg ccccgccagc gcctgcgcga tcgccgagcc
     3601 gataccgccg gacgctccag tgacgagcgc ggtcattcct gaaaggtcga acatgctttc
     3661 ctccgtcacc ccagcgaaag ctggggtcca tactgagccc gacacggcgc tacgaccgca
     3721 tggatgccag ccttcgctgg catgacgggc tagatctcct tcaccagcgc gtcgatgtcg
     3781 tccatcgtca ccacgctggt cgccttggcg tcgggggcga tgcgcttgac catggggccg
     3841 agcaccttgc ctccgaactc gacgaaatgg tcgacccccg cctccgccat cgccgccacg
     3901 ctctcgcgcc agcggaccat cccggtgacc tgctcgacga gcagccggcg gatctctccc
     3961 gggttctcaa ccggagccgc ggtgacgttg gcgtagagcg ggaccgccgg ctcctctatc
     4021 tcgacctcgg cgagggcctg ctccatctcc cgagcggcgg gctccatcag cggcgagtgg
     4081 aacggcgccg agaccgggag cagcaccgcg cgcttggcgc ctttctccct ggcgatctcg
     4141 agcgcgcgct cgacggcgcc gcggtggccg gagatcacca cctgcgaggg atcgttatcg
     4201 ttggcgacga cgcagacctc gccgtgggcg gcggcgtcgg cgacggcctg ggcgatctcg
     4261 acgctcgcgc cgagcagggc ggccatggcg ccctccccga ccggcaccgc ccgctgcatc
     4321 gcctggccgc gcagcttgag caggcgcgct gtcgtcggca ggtcgaacgc gccggcggcg
     4381 cacagcgcgc tatattcgcc gaggctgtgg ccggcgacga agtcggcttt gtcgatcgag
     4441 agccccg
//
LOCUS       DASVQZ010000208         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_719493, whole genome shotgun sequence.
ACCESSION   DASVQZ010000208 DASVQZ010000000
VERSION     DASVQZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719493"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1113)
                     /locus_tag="VF547_05700"
     CDS             complement(70..1113)
                     /locus_tag="VF547_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8262351.1"
                     /translation="MSHPIRLAAAFLALLAAPVAAAAAAEPSFVPVFRDNFPDPQVIV
                     HQGEFIAYATNAGINLPMLSSRDLVHWTWITRPDDPKKRLDGMPDLAPWVREGFTWAP
                     EVMKVGQRWLLYYTANHRKRDVQCLGVAIADNPRGPFRDTSAEPLVCQFDLGGTIDAN
                     PFRDSDGQLYLYYKSDGNRIGKRTTIWGQKLSSDGLKVEGSPVALVEDDRDWEWKLVE
                     APTMVRAPSGYQLFFSAAFFGWDPNERLSRYATGYATCAGPLGPCRDAPENPILHSFN
                     DKGAGCLSGPGHTSIFQAAGRHFIAFHAWAATKGCRKDVDKRYLYVAPLFWKDGKPQI
                     GLSLRDRSGGKPN"
     gene            1209..2339
                     /locus_tag="VF547_05705"
     CDS             1209..2339
                     /locus_tag="VF547_05705"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX8262352.1"
                     /translation="MRRPTLGSTVAAPPARAAAGVARSTFGRLPDGRSVEAITLANRA
                     GMEARIIAYGASLQALLVPDRDGALADVALGHPTIEPYLDQPQYFGSTVGRVANRIAQ
                     GRFTLDGKVHLTPVNNGPNALHGGTEGFDKKLWEVVETKEGEAASVVLRHVSPDGEMG
                     YPGTLTVTAAYSLNEAMELSVDYRATTDRPTLVNLSNHAYWNLAGEGSERGAMGHRLT
                     IPADCYLPTDEDSVPTGEFRPVEGTAFDFRRATPIAGRVRDASEDQIRFGKGYDHCWV
                     IARAPSSQPRLLARVEDPESGRAMEVLSNQPGLQFYSGNFLDATTSGKAGKLYRMGDA
                     IALEPQMVPDTPNRPEFGSVRLDPGQTYRNLIVWRFSAGGEG"
BASE COUNT          396 a          810 c          847 g          376 t
ORIGIN      
        1 agaggtcgtc ttgcgcggcc gcttgcgacg cggccaggag cgctgcgcac gccgacgcga
       61 ggagccggct caattcggct tgccgccgct gcggtcgcgc aggctgaggc cgatctgcgg
      121 cttgccgtcc ttccagaaca ggggcgcgac atagaggtag cgcttgtcga cgtccttgcg
      181 gcagcccttg gtcgccgccc aggcatggaa agcgatgaag tgccggccgg ccgcctggaa
      241 gatgctggta tggccgggcc cgctgaggca gccggccccc ttgtcgttga agctgtgcag
      301 gatcggattc tccggcgcgt ccctgcaggg gcccagcgga cccgcgcagg tggcgtagcc
      361 ggtggcgtag cgcgaaagcc gctcgttcgg atcccagccg aagaatgccg cggagaagaa
      421 aagctggtag ccggaggggg cgcgcaccat cgtcggcgcc tcgaccagct tccactccca
      481 gtcccggtcg tcctcgacca gcgcgaccgg agagccctcc accttgaggc cgtcgctcga
      541 cagcttctgc ccccagatcg tcgtgcgctt gccgatccgg tttccgtcgc tcttgtaata
      601 gaggtagagc tggccgtcgg aatcccggaa gggattggcg tcgatcgtgc cgccgaggtc
      661 gaactggcag acgagcggct ccgcggaggt gtcgcggaac ggacccctgg gattgtcggc
      721 gatggccact ccgaggcact ggacgtctcg cttgcggtga ttcgccgtgt aatagagcag
      781 ccagcgttgg ccgaccttca tcacctcggg ggcccaggtg aagccctccc tcacccaggg
      841 cgcgagatcc ggcatgccgt cgagccgctt cttgggatcg tcgggacgag tgatccaggt
      901 ccagtgcacg aggtcgcgcg acgacagcat cggcaggttg atcccggcgt tggtcgcgta
      961 ggcgatgaac tcgccctggt ggacgatcac ctgcggatcg ggaaaattgt ccctgaacac
     1021 cggcacgaac gaaggctcgg ccgccgcggc cgccgcgacc ggcgcagcga ggagggcgag
     1081 gaaagcggcg gcgaggcgga ttgggtggga catgggacat ccggattcgt ggcaggggag
     1141 cgcatcccct attatcatac gaatcgccgg agccaatgag cctggaacgc agctgccgga
     1201 gtgacgcgat gcgcagaccg accttgggaa gcacggtggc ggccccgccc gctcgcgccg
     1261 cggccggcgt cgcccgctcg acattcggcc ggctgcccga cgggcggagc gtcgaggcga
     1321 tcacgctcgc caaccgcgcc ggcatggaag cgcggatcat cgcctatggc gcttcgctgc
     1381 aggccctcct cgtcccggac cgcgacggcg cgcttgcgga tgtggcgctc ggccatccga
     1441 cgatcgagcc ctatctcgac cagccgcaat atttcgggtc aaccgtcggc cgcgtcgcca
     1501 atcgcatcgc gcagggccgc ttcacgctcg acggcaaggt ccatctcacg ccggtcaaca
     1561 acggtccgaa cgcgctccac ggcggcaccg agggattcga caagaagctg tgggaggtgg
     1621 tcgagacgaa ggagggagag gcggcgagcg tcgtcctgcg ccacgtcagc cccgacggcg
     1681 agatgggcta tccggggacg ctgacggtca ctgccgctta ttctctcaac gaagccatgg
     1741 aattgtcggt agactatcgc gcgacgaccg accggcccac cctcgtcaac ctttccaacc
     1801 acgcttactg gaacctggcc ggcgaaggct ccgagcgggg ggcgatgggg catcggctta
     1861 ccatccccgc ggattgctat ctgccgaccg acgaggattc ggtccccacc ggcgagttca
     1921 ggcccgtgga gggcaccgcc ttcgatttcc gccgtgccac gccgatcgcc gggcgggttc
     1981 gcgacgcctc ggaggaccag atccgattcg gcaaaggcta tgaccattgc tgggtgatcg
     2041 cgcgcgcgcc gtcgtcgcag ccgcggcttc tcgcccgggt cgaggatccg gaatcggggc
     2101 gcgcgatgga ggtcctgtcg aaccagccgg gcctccaatt ctattccggg aacttcctcg
     2161 acgcgacgac ctcgggcaag gccggaaagc tctaccggat gggcgatgcg atcgctctgg
     2221 agccgcagat ggtcccggac acgccgaacc ggcccgaatt cggatccgtg cgcctcgatc
     2281 cggggcagac gtaccggaac ctgatcgtct ggcgcttctc ggccggagga gaaggctagg
     2341 ctggcccgcc cgcctcggcg aacaggacca tcacctcgcc gttgatcgcc gccatggcgg
     2401 agcgggcgcc gtcggggttg cgggcccgc
//
LOCUS       DASVQZ010000209         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_525143, whole genome shotgun sequence.
ACCESSION   DASVQZ010000209 DASVQZ010000000
VERSION     DASVQZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525143"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VF547_05710"
     CDS             complement(<1..1100)
                     /locus_tag="VF547_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX8262353.1"
                     /translation="MAKRPPFEAIHRHGFVRVAAASPTGSSGDVAFNVDQAIALARDA
                     HDKGCDLVVYPELNISSYAVDDLFLQEAFLDAVEDGIARFRDSTAKLSPVLVIGAPLR
                     RNGRLYNCALAVSRGRILGVVPKSFLPNYREYYEKRWFVSGIGLNGIDIAVAGEIVPF
                     GTDLIFAAEDLDDFIFHVEICEDYWAAQPPSTVGALAGALILCNLSASNITIGKADER
                     KLLCASQSSRCCAAYVYAASGPGESTTDLAWDGQGSIYETGELLCDSGRFDLEPELCS
                     ADIDVQRLRLERMRMPTFNDNAVAAGHPEKNARRIHFEHRPAFADIGFRRKLRRFPYV
                     PNRLEQLDSDCFEAFNIQVQGLARRFQATSGD"
     gene            complement(1100..1600)
                     /locus_tag="VF547_05715"
     CDS             complement(1100..1600)
                     /locus_tag="VF547_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX8262354.1"
                     /translation="MNDTETLSPVLPDETEEATRRLLQKASERGLRLATAESCTGGML
                     ASLLTDVQGVAHAFERGFVTYTNEAKSEMLGVPMALIEEKGAVSREVALAMAEGALER
                     SRANIALSITGFADKGDEPGLVHFGCARGGRATAHREEHFGPGGRGATRVKCMRVAVE
                     MMTEML"
     gene            1650..2192
                     /locus_tag="VF547_05720"
     CDS             1650..2192
                     /locus_tag="VF547_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8262355.1"
                     /translation="MFQSPNEESDEMVTERKMDATHLLAEDHRKVEELFEQFEKASGD
                     GRKEKIARQICTELKVHSMIEEEIFYPALRGKIEDDDLDEAYVEHDGAKVLINEIEAG
                     GPDEEFYDAKVKVLQEQIEHHVEEEEKSRGNLFQQARAADVDLEALGRQMAARKEELM
                     RLAEAEGLPPAKPATMSTEG"
     gene            2193..2480
                     /locus_tag="VF547_05725"
     CDS             2193..2480
                     /locus_tag="VF547_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262356.1"
                     /translation="MRKGAVITGLAAACAAGLGYLAARLWRRPDEPGGPGARPAISGS
                     GGEGAVGDSGAARSAGPDAMRDPPKDWEQVDQASDESFPASDPPALNPHVD"
BASE COUNT          440 a          860 c          926 g          364 t
ORIGIN      
        1 ttgtcgcccg aggtcgcctg gaagcggcgg gcgaggccct ggacctggat gttgaacgcc
       61 tcgaagcagt cggaatcgag ctgctcgagc cggttgggga cataagggaa gcgccgcagc
      121 ttccgccgga agccgatgtc ggcgaaggcc gggcggtgct cgaaatggat ccgccgcgcg
      181 ttcttctcgg gatggccggc ggcgaccgca ttgtcgttga aggtcggcat ccgcatccgc
      241 tccagccgca ggcgctggac gtcgatgtcg gccgagcaca gctccggctc gaggtcgaag
      301 cggccggaat cgcagagcag ctcgcccgtc tcgtagatcg agccctggcc gtcccaggcg
      361 aggtcggtgg tgctctcgcc cggccccgag gcggcgtaga cataggccgc gcagcagcgg
      421 ctcgactggg aggcgcagag aagcttgcgc tcgtcggcct tgccgatcgt gatgttggac
      481 gcgctgaggt tgcacaggat cagcgcgccg gcgagcgccc cgaccgtcga gggcggctgc
      541 gccgcccaat aatcctcgca gatctcgacg tggaagatga agtcgtcgag gtcctcggcg
      601 gcgaagatca ggtcggtgcc gaacggaacg atctcgccgg cgacggcgat gtcgatgccg
      661 ttgaggccga ttccggagac gaaccagcgc ttctcgtaat attcgcggta attgggcagg
      721 aagctcttcg ggacgacgcc gaggatccgg ccgcgcgaga cggccagggc gcaattgtag
      781 agccggccgt tgcgcctcag cggcgcgccg atcaccagca ccggcgagag cttggcggtc
      841 gagtcgcgga accgggcgat cccgtcctcg acggcgtcga ggaaggcctc ctgcaggaac
      901 aggtcgtcga ccgcatagga cgagatgttg agctcggggt agacgacgag atcgcagccc
      961 ttgtcgtggg cgtcccgggc gagggcgatc gcctggtcga cgttgaaggc gacgtcgccc
     1021 gaggagccgg tcgggctcgc ggccgcgacc cggacgaagc catggcggtg gatcgcctcg
     1081 aaggggggcc tcttggccat cacagcatct ccgtcatcat ctcaacggcg accctcatgc
     1141 atttgacgcg cgtagcacct ctgccgcccg gaccgaaatg ctcctcgcga tgggcggtgg
     1201 cccggccgcc gcgcgcgcag ccgaaatgga cgaggcccgg ctcgtcgccc ttgtcggcga
     1261 agccggtgat cgacagcgcg atgttggcgc gcgaccgctc gagcgcgccc tcggccatgg
     1321 ccagcgccac ctcccgcgac accgccccct tctcctcgat cagcgccatc ggcaccccca
     1381 gcatctcgga cttggcctcg ttggtatagg tgacgaagcc ccgctcgaag gcgtgggcga
     1441 cgccctgcac gtcggtgagc agggacgcga gcatcccgcc cgtgcagctc tcggcggtgg
     1501 cgagccgaag gccgcgctcc gacgccttct ggagcaggcg ccgggtggct tcctcggtct
     1561 cgtcggggag caccggcgac aaggtctcgg tatcgttcaa aacttcttcc tcgcgcgctt
     1621 tgcgccaagg aaccggtccc tagcgccgaa tgttccagag cccaaacgag gagagcgacg
     1681 agatggttac cgagcgcaag atggacgcca cccacctgct ggcggaggat caccgcaagg
     1741 tcgaggagct gttcgagcag ttcgagaagg cgagcggcga cggccgcaag gagaagatcg
     1801 cccgccagat ctgcaccgag ctcaaggtcc actcgatgat cgaggaggag atcttctacc
     1861 cggcgctgcg cggcaagatc gaggacgacg atctcgacga ggcctatgtc gagcatgacg
     1921 gcgccaaggt gctgatcaac gagatcgagg ccggcgggcc ggacgaggaa ttctacgacg
     1981 ccaaggtgaa ggtgcttcag gagcagatcg agcaccatgt cgaggaggag gagaagagcc
     2041 gcggcaacct cttccagcag gcccgcgccg ccgacgtcga cctcgaggcg ctcggccgac
     2101 agatggcggc gcgcaaggag gagctgatgc gcctcgccga ggccgagggc cttccgcccg
     2161 ccaagcccgc gacgatgagc acggagggct gaatgaggaa gggggccgtc atcaccggcc
     2221 tcgccgcggc ctgcgcggcg ggcctgggct atctcgcggc gaggctctgg cgccggcccg
     2281 acgagccggg cgggcccggc gcccgtccgg cgatcagcgg ctccggcggc gaaggcgcgg
     2341 tcggcgattc gggcgcggcg cggtcggcgg gccccgacgc catgcgcgac ccgccgaagg
     2401 actgggagca ggtcgaccag gcctccgacg aaagcttccc cgccagcgac ccccccgccc
     2461 tcaaccccca cgtcgactga tcgcaaattc ctcccccgga tttccggggg aggggaacgc
     2521 gcgaagcgcg gtggaggggt tgttcgcgct ctgaaagaac ccctccacca ggcttcgcct
     2581 ggtccccctc
//
LOCUS       DASVQZ010000210         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_912752, whole genome shotgun sequence.
ACCESSION   DASVQZ010000210 DASVQZ010000000
VERSION     DASVQZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912752"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..>1609
                     /locus_tag="VF547_05730"
     CDS             196..>1609
                     /locus_tag="VF547_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262357.1"
                     /translation="MLQGEPEPDGEVAALFAPAQGRLPAAEIGLILAGPALQLESGER
                     RLDLRLTLSGLQWPESAEPSLFSGLAEAALQPSLLTDEGWHALGAADASLSTLSGDTA
                     ELVLRLLLPRTAPAAAAGAPALRLLLVQDAAVEGAPEGLTPLAVLAAARVEAVALSVA
                     VEDLGDLAVSTSAGAAIAAPGLLAFGHAPSPGAYLQLDHPVLDRGRLDRVAVTLRWAD
                     PPPHPDGFTGWYRQYRVDLDRRLHDEGAPLLRNDSFAVRLSAPVGQGRKEGEYFLFST
                     GGGDGRVAPLSTFSLTAREGAEPGRRGASGVRMTLSAPEHGFGDLLYPVNVARATQLV
                     ASGAGGARRRGFWRWLLALLRKLLAAPLKPLAAMGKGLKKLWSLFSLTPDAFQPEPEP
                     EALPPADTERADDLAALLPNPPWRAVLAAVRLDYTASFALDRQGSAGDIHLMHWSGWD
                     AAVPVPWKDGARLLPDLPEQA"
BASE COUNT          192 a          575 c          608 g          234 t
ORIGIN      
        1 aggcctgcgg cggccagacc gggagcgccg ccgccccgga tcgccgcggg gcatatgttc
       61 gaggcgggac tcgacgtcac cggccggccg atccgtttcg cggccgagaa cgcgctgaag
      121 gtaacgggcg cgcgggtgaa ggaggtccgg ctctggcgcc cggagacgag cggcggccgg
      181 cggagccgcg tcgggatgct gcagggcgag ccggagcccg acggcgaggt cgcggcgctg
      241 ttcgcgcccg cgcaggggcg cctgccggcg gccgaaatcg gcctgatcct cgcaggtccc
      301 gctctccaac tggaatcggg cgagcggcga ctggacctgc ggcttacgct ttccggcctc
      361 caatggcccg agagtgcgga accatcgctc ttctccggcc tggcggaggc cgccctgcag
      421 ccgtcgctgc tgaccgacga gggatggcac gccctcggcg cggcggatgc ctcgttatcc
      481 accctttcgg gcgacacggc cgagcttgtg ctccgcctcc tcctcccgcg cacggcgccg
      541 gcggcggcag ccggggcgcc ggcattgcgc ctgctgctgg tccaggacgc cgccgtcgaa
      601 ggtgcgcctg aggggctgac gccgctcgcg gtgttggcgg cggctcgcgt ggaggcggtg
      661 gcgctgagcg tggcggtcga ggatctcggt gacctcgccg tctcgacgag cgccggggcg
      721 gcgatcgcgg cgcccggact tctcgccttc ggccatgctc cctcgccggg cgcgtatctc
      781 cagctggatc atcccgttct cgaccggggg cggctcgacc gggtcgcagt gacgctgcgc
      841 tgggccgacc cgccgccgca cccagacggc ttcaccggct ggtaccggca gtatcgggtc
      901 gatctcgacc ggcgtctgca cgacgagggc gcgccgctgc tccgcaacga cagcttcgcg
      961 gtccggctgt ccgcgccggt cgggcagggg cgaaaggaag gggagtattt cctcttctcc
     1021 accggcggcg gcgacgggcg ggtcgcgccg ctctcaacct tctcgctcac cgcgagggag
     1081 ggggcagagc cgggacgccg cggggcatcg ggcgtgcgga tgacgcttag tgcacccgag
     1141 cacggcttcg gtgacctgct ctacccggtc aacgtcgcgc gggcgacgca gctggtcgcg
     1201 tccggcgccg gcggcgcgcg gaggaggggc ttctggcggt ggctgctcgc tttgctgcgc
     1261 aagctgctgg cggcgccgct gaagccgctc gcggccatgg gcaagggcct caagaaactg
     1321 tggagcctgt tctccctcac gccggacgcg ttccagccgg agccggagcc ggaagcactg
     1381 ccgcccgccg acacggagcg ggccgacgat ctcgccgcgc tccttcccaa tccgccgtgg
     1441 cgggcggtcc tcgccgctgt ccggctggat tacaccgcct ctttcgcact cgatcgacaa
     1501 gggagtgcgg gcgacatcca cctgatgcac tggtcgggct gggacgcggc cgttccggtg
     1561 ccctggaagg acggcgcgcg cctgctcccc gatctgccgg agcaggcct
//
LOCUS       DASVQZ010000211         7331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_430559, whole genome shotgun sequence.
ACCESSION   DASVQZ010000211 DASVQZ010000000
VERSION     DASVQZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430559"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..765)
                     /locus_tag="VF547_05735"
     CDS             complement(313..765)
                     /locus_tag="VF547_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX8262358.1"
                     /translation="MFNDHDVTVLNSLIETTIDSVDGYRHSADEATSDTFTAIFRQRA
                     EEREQVVAKLRDRVRELGGTPEEDGGLLAKAHRQFLSLRDAVTGRDDKAVIAEVDRGE
                     SYLNGKWEAALADTELSSETQRLIRDCYQSVRSGHEQWEQINKSIKAS"
     gene            879..1091
                     /locus_tag="VF547_05740"
     CDS             879..1091
                     /locus_tag="VF547_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262359.1"
                     /translation="MADTREERGRPAGSDRESGEVHGSGSGAGGGNPGEDYDQDPMAG
                     SGSEPPHGPRAAGDSAERPIDPDEGK"
     gene            1088..1279
                     /locus_tag="VF547_05745"
     CDS             1088..1279
                     /locus_tag="VF547_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262360.1"
                     /translation="MTMGKGHPLPGSDEARHDEIPESLAEDKGRGDDPKPVVQPGREA
                     ANPDGEPYRYDDNGRAEKF"
     gene            1380..1979
                     /locus_tag="VF547_05750"
     CDS             1380..1979
                     /locus_tag="VF547_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262361.1"
                     /translation="MTNQNNQDGGQFGGNSQQGGQQGGGTTGTDFGQSGGTGGQTGRE
                     DQQGGTSGGQGGDLRQGGGSSGGQSGGDFDESQGGAGTGSDWQQDQQGGQSGHQGGFQ
                     QDQQGQAGGQQGGSEWQQDQQGGQQGGSEWQQDQQSGQQGGSEWQQGGQSGQQGGFQQ
                     DQQGGQRKGGADVEFQQDQQSNQDRGQGQGGIEFESDQR"
     gene            2110..2985
                     /locus_tag="VF547_05755"
     CDS             2110..2985
                     /locus_tag="VF547_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262362.1"
                     /translation="MAKQNSDQGGSSGGSKSKKGGAGAYADVLKEAGERAADLARNPV
                     ARSMLAAGLVTAAAALTANARVRKTVRDAGRDALDTAEAAADSASKIGAAIVTAATDA
                     VRRLLAGADEAGSDTGGGGSDRAKRASPGGKAARGAAKPGGGRGKAAAAGAGRGGAAK
                     SAGAGRGSGTSSAAGTKRAGAQSAGSRGTATKSSGAKSAGTKSAGARSGGSRAASSGS
                     GGSGAKATGSGGSATGGKGRSGASGSTGSSGRRASTGGGGRGGGGRKGSGAGASASGG
                     SSGGAGGPDGTGGQG"
     gene            3148..3408
                     /locus_tag="VF547_05760"
     CDS             3148..3408
                     /locus_tag="VF547_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262363.1"
                     /translation="MHKLTVRLSSAALLLAAAATPVAAAGPASSPSNEASVANSAAPA
                     HSTKSTPDEPKKICRTIETTSSRLKAKRVCLTREQWRNAKYN"
     gene            complement(3641..4966)
                     /locus_tag="VF547_05765"
     CDS             complement(3641..4966)
                     /locus_tag="VF547_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX8262364.1"
                     /translation="MIDGAGKCLKAERRLLLFGTAAALIVTVAGPAHAAGFYLQEQSV
                     KGWGRANSGEVADQGAASLWWNPAAIAGLKRPEATFGATGILPRGRVSDEGTLIDRPG
                     SPPAPVGGIASLSDPIQRGVAPHNAFALPLGDRIAIGLAVTAPFSFTSDYDPSGWQRY
                     SAIRSRLLTLDFQPSIAFAATDWLSVGGAVNVQYSDAWLSNALPNLAPGSPDGRLRLT
                     GNGWDLGWSAGVQLRPTRRSTIGIAYKSAVSHKLEGPVEIAGLAGPLAARNQRVQTVA
                     RFTTPWQLIFGARVGVSEGLTLNAQAVRFGWSKFDQIDLAAPLNSAIVENYKDTWSAA
                     VGLDQALGERLTLRAGIQIDPTPTRDAGRDPRVPDGDRIDYNVGASYRLSERLSLDAA
                     AGYTDLENGPISRDERFYAGTAAETDILTEGRAHGQSVLVVAVGGRLSF"
     gene            5209..6147
                     /locus_tag="VF547_05770"
     CDS             5209..6147
                     /locus_tag="VF547_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8262365.1"
                     /translation="MNQHFSLPTSDEAITVAVAAKSLGGGLPLQHRERPAGTRLFDLG
                     QLDVRWEAALGTLWTYMTPVDRPNFNRPMLRDFQRWQAEIGREFADPEEGLRYLVLGS
                     RFPGVFNLGGDLKLFAGFIAAGDRDGLIRYGRDCVSILHNNMRRLELPIVTIALVQGD
                     ALGGGFEAVLSFNVIVAEKGARFGLPEIAFGLFPGMGAHSLLSRKIGLARAEEMMLSN
                     RLYTAEDMYEMGIVHVLAEPGRGEEAVRAYIAKNGRRQVGHRGIYNASALAAPVTLEE
                     LNAIVEVWADSALCLSDADLKLMRRLGDAQTRLAAA"
     gene            complement(6128..>7331)
                     /locus_tag="VF547_05775"
     CDS             complement(6128..>7331)
                     /locus_tag="VF547_05775"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX8262366.1"
                     /translation="FKEINDSLGHPTGDGVLCTVARQLGSIVDGRGSVARFGGDEFVL
                     LARGTKPFFAEELAREVMRGLSVPMTVEGTSIQVTGSIGAAVAPEHGSEADILLQHAD
                     MALYHAKANGRNDFCMFESKMQDEFQQKRRLEAALKHAIEKGELEVYYQPIVDLKTGK
                     VSCCEALLRWHHPELGMVSPAVFIPLAESTGLISPISHWVLYQACAVAAKWPEDVSIA
                     VNMSPALLKDLHLSHMILSALYRSGLAARRLELEITESVLLEENAQSNSLIREFQRIG
                     LKLSIDDFGTGYSSLTYLKKYRFDKIKIDTSFIADVTKSREARAIIHALVGLAAELDM
                     EIVAEGIETETQFGYVTGAGCTAAQGFFLGRPVPESVINRRLEAQGRGEEWTEADPEV
                     EGLRRRQA"
BASE COUNT         1185 a         2503 c         2576 g         1067 t
ORIGIN      
        1 aaacgggcat tcttgtggat gttcatccga ctctcctgag gatctgaagc ttcgcaacct
       61 cagtctcccc ggttcgggtc ggatggacaa cctactgaaa gctcacagct agagcgttga
      121 cggattaggt tgaatcgacg aggccgttcg ccctgagcct gtcgaagggc cttgcttctc
      181 gttctccaga ggcaaggcga gggcttcgac aagctcagcc cgaacggagt aagtcgaacc
      241 gactaccgaa cgctctagcc gggaaaggtt cgcctcccca cgcgccgaca cgcgcgggga
      301 ggatgagccc acttagctcg ccttgatgct cttgttgatc tgctcccact gctcgtggcc
      361 ggagcgcacc gactggtagc agtcgcggat caggcgctgg gtctcggacg agagctcggt
      421 gtcggcgagc gccgcttccc acttgccgtt cagatagctc tcgccgcggt cgacctcggc
      481 gatcaccgcc ttgtcgtcgc ggccggtgac ggcgtcgcgc agcgagagga actgccggtg
      541 ggccttggcg agcaggccgc catcctcctc gggcgtgccg ccgagctcgc ggacgcgatc
      601 gcgcagcttc gcgacgacct gctcgcgctc ctcggcgcgc tggcggaaaa tggcggtgaa
      661 ggtgtcgctg gtcgcctcgt cagcggaatg gcgatagccg tcaacgctgt cgatggtggt
      721 ctcgatgagg ctgttgagga cggtgacgtc gtggtcgttg aacatggctg actccggtct
      781 tcaggttgcg cgccctaaac ccgcggcggg ccgccaaagg tccgtgccgg aacgggaaaa
      841 gcgccaggac gttcaccggg gcggaaggag ccgacgccat ggccgacaca agggaggagc
      901 ggggacggcc cgccggctcc gatcgggaga gcggcgaggt ccacggctcc ggctcgggcg
      961 ccggcggcgg caatcccggc gaggattacg accaggaccc gatggccggc tccggctcgg
     1021 agccgccgca cgggccgcgc gccgcgggcg attccgccga gcgcccgatc gatcccgacg
     1081 aagggaaatg accatgggca aaggccaccc cctcccgggc agcgacgagg cgcggcacga
     1141 cgagatcccc gagagcctgg ccgaggacaa gggccgcggc gacgatccca agccggtcgt
     1201 ccagccggga cgcgaggccg ccaatcccga cggggagccc tatcgctacg acgacaacgg
     1261 ccgcgccgag aagttctgac gggacgccgc gccggggcac cattccggcg cgacgccggg
     1321 acccgaccgt cttaaccgac gttaatcagg cttccagttc accatgcagg agcatgacca
     1381 tgacgaacca gaacaatcag gacggcggcc agttcggagg caattcgcag caaggcggcc
     1441 agcagggcgg cgggaccacc gggaccgatt tcggccagtc gggcggcacc ggcggccaga
     1501 ccggccgcga ggaccagcag ggcggaacct cgggcggcca gggcggcgac cttcgccagg
     1561 gcggcggcag ctccggcggc cagtccggcg gggacttcga tgaatcgcag ggcggagccg
     1621 gcaccggttc cgactggcag caggaccagc agggcggcca gagcggccac cagggcggct
     1681 tccagcagga tcagcagggt caggccggcg gccagcaggg cggctccgaa tggcagcagg
     1741 accagcaggg cggccagcag ggcggctcgg agtggcagca ggaccagcag agcggccagc
     1801 agggcggctc ggaatggcag cagggcggcc agagcggcca gcagggcggc ttccagcagg
     1861 accagcaggg cggccagcgc aagggcggcg cggacgtcga gttccagcag gaccagcagt
     1921 ccaaccagga tcgcggccag ggccagggcg gcatcgagtt cgagagcgac cagcgctagc
     1981 tgacgctcgg cgaacgacag gaagggccgt cctcgcgggc ggcccttctt cgcgtgcgcg
     2041 gccgagggaa ccactcgccg cgctgctcgt cttctgttac gcctcagtta taaaaagacg
     2101 gagaccgaca tggccaagca gaattcggac cagggcggct cgagcggcgg ctcgaaatcg
     2161 aagaagggcg gcgccggcgc ctatgccgac gtgctcaagg aggcgggcga gcgcgccgcc
     2221 gacctcgccc gcaacccggt cgcccgatcg atgctcgccg ccgggctggt caccgccgcg
     2281 gccgcgctca ccgccaatgc gcgggtgagg aagacggtgc gcgacgccgg ccgcgacgcg
     2341 ctcgacacgg ccgaggccgc ggcagacagc gcgagcaaga tcggagcggc gatcgtgacc
     2401 gcggccaccg acgccgtccg gcgtctactg gcgggcgccg acgaagccgg ctcggacacg
     2461 ggcggcggcg gctcggaccg ggccaagcgc gccagccccg gcggcaaggc ggcgcgcggc
     2521 gccgccaaac cgggcggcgg ccgcggcaag gctgcggcgg cgggggccgg ccgcgggggc
     2581 gccgcaaaga gcgccggcgc cggccgcggc tccggcacct cttccgccgc gggcacgaag
     2641 cgcgcgggcg cccagagcgc cggcagccgg ggcacggcaa ccaagagctc cggcgccaag
     2701 agcgcgggga cgaagagcgc cggcgccagg agcggcggat cccgcgccgc gtcctccggt
     2761 tccggcgggt ccggcgccaa ggccacgggg tccggcgggt cggccacggg cggcaagggc
     2821 cggtcggggg cgagcggttc gaccggcagc tccgggcggc gggcctcgac cggcggcggc
     2881 ggccgcggcg gcggcgggcg caagggctcc ggcgccggag cctcggcctc gggcggcagc
     2941 agcggcggcg ccggcggccc ggacggcacc ggcggccagg gctgaacgcc cctttcggcg
     3001 cagctttcgc cgagtcctct cccgcctttc gcgggagagg accaggccgg attgtccgtt
     3061 tccggactac ccacttgacg cggagcttcg gatcggcttt ttgaccgggc ccactggggg
     3121 gcaaatttcc tgaggaatgt catgcacttg cataagctta ccgtgcgcct ttcgagcgcc
     3181 gccctgctgc tcgccgctgc ggcgaccccg gtcgcggcag ccggtcccgc ctcgtcgccc
     3241 tccaatgagg ccagcgtcgc caactcggcc gcccccgcgc attccaccaa gtcgactccg
     3301 gacgagccca agaagatctg ccgcacgatc gagacgacga gctcgcggct gaaggcgaag
     3361 agggtctgcc tcacccggga gcagtggcgc aacgccaagt ataattgagg ccgggcgcaa
     3421 gcgtcccggc gaggccggac ccagccgcgc ggcaaacggc cgcggccgga tcgatccgct
     3481 tgctgaccag tctagaacgg tgagggatca ggtcgaatcc ctctcgcccg ttcgccctga
     3541 gcctgtcgaa gggctttcct tgtcgttcca tgagagaaga ggagagggct tcgacaagct
     3601 cagcccgaac ggggatagcg cagtctgggc gccgggcgcg ctagaagctg agccggccgc
     3661 cgacggcgac gacgagcacg ctctggccgt gcgcccgacc ttcggtgagg atgtcggtct
     3721 cggccgcggt gccggcatag aagcgctcgt cgcggctgat cggtccgttc tcgaggtcgg
     3781 tatagccggc cgcggcgtcg agcgagagac gctcgctcag gcggtagctc gcccccacat
     3841 tgtagtcgat ccggtcgccg tccggcacgc gcggatcgcg gccggcgtcg cgggtcggag
     3901 tcggatcgat ctggattccg gcgcgcagcg tcagccgctc gccgagcgcc tggtcgaggc
     3961 cgacggcggc gctccacgta tccttgtaat tctcgacgat ggcgctgttg agcggcgcgg
     4021 ccagatcgat ctggtcgaac ttgctccagc cgaaacggac cgcctgggcg ttgagggtaa
     4081 ggccttcgct cactccgacg cgtgcgccga aaatcagctg ccacggcgtg gtgaagcggg
     4141 cgacggtctg cacgcgctgg ttgcgcgccg ccagcggccc ggccagcccg gcgatctcga
     4201 ccggtccctc gagcttgtgg cttaccgccg acttgtaggc gattccgatc gtcgaccgcc
     4261 gtgtcgggcg aagctggact ccggcgctcc accccaggtc ccagccgttg ccggtgagcc
     4321 gcagccgccc gtcgggggag cccggcgcca ggttcgggag cgcgttggag agccaggcgt
     4381 cgctatactg gacgttgacc gccccgccga cgctcagcca gtcggtcgcg gcgaaggcga
     4441 tcgaaggctg gaagtccagc gtgagcaagc ggctccgaat cgcggaatag cgctgccacc
     4501 ccgaagggtc gtagtcgctg gtgaagctga acggggcggt gacggccaag ccgatcgcga
     4561 ttcggtcgcc cagcgggagg gcgaacgcat tatgcggcgc aacgccgcgc tggatcgggt
     4621 ccgaaagcga tgcaattccc cctaccggcg ccgggggaga cccgggacgg tcgatcagcg
     4681 tgccctcgtc gctgacccgg ccgcgcggca ggatgccggt cgctccgaat gtcgcttcgg
     4741 gtcgcttgag ccccgcgatc gccgccgggt tccaccacag cgacgccgcg ccctgatcgg
     4801 ccacttcgcc ggagttcgcg cggccccatc ccttaacgct ttgttcctga aggtaaaatc
     4861 cggcggcatg agccgggccg gcgacggtta cgatcagcgc cgcggccgtc ccgaaaagca
     4921 acaggcgccg ctcagccttc agacattttc ccgccccgtc gatcatcttg cccccacttt
     4981 gaacccctga cgcaccccgg cgacgcagga ggattagtgt cgaaatcctt gtatctcaac
     5041 cgttttcggt taaatccgaa acggatattt gttatgtttc gcaattcatc ataacgagtc
     5101 atcccagcaa cactgatacg gttaacgcgg caaaccggtt cgagcctgat tgttcccccg
     5161 ccgccgatgt gcttaccttc gtgaaaatca gatgcttggg gcacaacgat gaatcaacat
     5221 ttcagccttc ctacctccga cgaggcgatc acggtagcgg tcgccgccaa gagtctgggg
     5281 gggggcctgc ccctccagca tcgcgagcgc ccggccggca cccggctgtt cgacctcggc
     5341 cagctcgacg tccgctggga ggccgcgctc ggcaccttgt ggacctacat gacgccggtc
     5401 gaccggccca atttcaaccg tccgatgctg cgcgacttcc aacgctggca ggccgagatc
     5461 ggccgcgagt tcgccgatcc cgaggagggg ctccgctatc tcgtgctcgg ctctcgattc
     5521 ccgggcgtgt tcaacctcgg cggcgacctg aagctgttcg ccggattcat cgccgccggc
     5581 gaccgcgacg gactgatccg ctacggccgc gactgcgtct cgatcctcca caacaacatg
     5641 cgccgcctcg agctgccgat cgtcaccatc gccctcgtcc agggggacgc gctcggcggc
     5701 ggcttcgagg cggtgctgag cttcaacgtc atcgtcgccg agaaaggcgc gcgcttcggc
     5761 ctgcccgaga tcgctttcgg cctgttcccc gggatgggcg cgcacagctt gctgtcgcgc
     5821 aagatcggcc tcgcccgggc cgaggagatg atgctctcca accggctcta cacggccgag
     5881 gatatgtacg agatgggcat cgtccacgtc ctggccgagc cggggcgcgg cgaggaggcg
     5941 gtgcgcgcct acatcgccaa gaacggccgc cgccaggtcg ggcatcgcgg catctacaac
     6001 gcctcggccc tcgcggcccc ggtcacgctc gaggagctca acgcgatcgt cgaggtgtgg
     6061 gccgacagcg cgctgtgcct cagcgacgcg gacctcaagc tgatgcggcg gctcggcgac
     6121 gcgcaaacta ggcttgccgc cgcctgaggc cttccacttc cggatcggcc tctgtccatt
     6181 cctcgccgcg gccctgcgcc tcgagtcggc ggttgatcac cgattcgggc acgggccggc
     6241 ccaggaagaa gccctgggcg gcggtgcagc cggcgcctgt gacgtagccg aactgggtct
     6301 ccgtctcgat cccctcggcg acgatctcca tgtcgagctc cgcggcgagc ccgaccaggg
     6361 cgtggatgat cgcccgcgcc tcccggctct tggtgacgtc ggcgatgaag ctggtgtcga
     6421 tcttgatctt gtcgaaccgg tatttcttca aataggtgag cgatgaatag ccggtcccga
     6481 aatcgtcgat cgaaagcttg aggccgatcc tctggaactc gcggatcagc gagttggact
     6541 gcgcattctc ctcgagcagc acgctctccg tgatctccag ctccagccgc cgcgccgcga
     6601 gccccgaacg gtagagcgcc gagaggatca tgtggctgag gtgcaggtcc ttgagcaggg
     6661 ctggcgacat gttgacggcg atgctgacgt cctccggcca tttcgccgcg acggcgcagg
     6721 cctggtacag cacccaatgg ctgatcggcg agatcagccc agtgctctcg gcgagcggga
     6781 tgaagacggc ggggctgacc atgcccagct cgggatggtg ccagcgcagc agggcctcgc
     6841 agcagctgac cttgcccgtc ttgagatcga cgatcggctg gtaatagact tcgagctcgc
     6901 ctttctcgat tgcatgcttc agcgccgcct cgagcctgcg cttctgctgg aactcgtcct
     6961 gcatcttgct ctcgaacatg cagaagtcgt tccgcccgtt cgccttggcg tggtagagcg
     7021 ccatgtcggc gtgctggagc aggatgtcgg cctcgctgcc gtgctcgggc gccaccgccg
     7081 cgccgatcga tccggtcacc tggatcgagg tgccctcgac cgtcatcggc accgagaggc
     7141 cccgcatcac ctcgcgcgcc agctcctcgg cgaagaaggg cttcgtgccg cgggcgagca
     7201 ggacgaactc gtcgccgccg aagcgggcga cgctgccgcg gccgtcgacg atcgagccga
     7261 gctgccgggc gacggtgcag agcacgccgt cgccggtcgg gtggccgagc gaatcgttga
     7321 tctccttgaa g
//
LOCUS       DASVQZ010000212         8247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_815167, whole genome shotgun sequence.
ACCESSION   DASVQZ010000212 DASVQZ010000000
VERSION     DASVQZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815167"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..2158
                     /locus_tag="VF547_05780"
     CDS             479..2158
                     /locus_tag="VF547_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8262367.1"
                     /translation="MSLAEVDALLTAPGSRLETAEAVIRGIPTRVWKNAPPSLAFLVR
                     HSRTHGDRPFTVYEEERIGFEASFRATAALARELVRLGVGPGDRVAIAMANLPEWPAA
                     FFAIVSLGAIAVPLNAWWTGAELEYGLADSGSKVLICDGPRWQRIEPHRPALTALEHI
                     IVSRGPAPAGGARLEDLIGAPPAWAALPDSDLPEAAVAADDPATILYTSGTTGRPKGA
                     LGTHRNLLTNILSVGYSNARAALRRGEAPPEPAPKVALMVIPLFHCTALSAMLMGTMV
                     AGHTIVFMRKWDPLEAMAIIERERVQLTGGVPTIAWQILEHPERSRFDLSSLETISYG
                     GAPAAPELVRRIWEEFGALPGNGWGMTETTATVTSHVGEDYLNRPDSAGPPVPVAELR
                     IMSADGERALPVGEIGELWAKGPQIVQGYWNNPEATAATFVDGWVRTGDLARLDEEGF
                     LYIVDRAKDMVIRGGENIYSIEVENVLFDHPAVTDAALIGLPHRTLGEEPAAVVHLAP
                     GATASEEELKAWVRERLALFKTPVRIAFLPQPLPRNANGKILKQELKALFD"
     gene            complement(2257..3501)
                     /locus_tag="VF547_05785"
     CDS             complement(2257..3501)
                     /locus_tag="VF547_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX8262368.1"
                     /translation="MIDVLVPAEQEGTQAVVRAWLRQVGDRVEVNDPLVELETDKVAM
                     EVPAPAAGVLREILLDTDAEAVPGAVLGRIAPVAEAAEHEPSGTPAKAGAQPSKGASG
                     PRSSPGNEAHREHRLSPSVRRALVQHDIDPSRIEGTGRGGRITRADVDRAVEESAKAG
                     RAVEGVIPAQVGDPSASLGTGIRSHSVPHDRMRLKIAGNMLRSVTEAPHVTAMFEADF
                     GAVVAHRERHKEAFARKGVKLTYSAYIVAAAAEAMKVAPAINSRWHEDRLEIFDDVNI
                     GVGTALGEKGLIVPVLRKVQTLSLKGIAAGLDELVEKARREQLTGKDVSGGTFTISNH
                     GVSGSLFAAPIIINQPQSAILGVGKLQKRVVVREVDGADSIQIRPMAYVSLTIDHRVV
                     DGHQTNAWLTRFVEVLEGWKAD"
     gene            complement(3644..5725)
                     /locus_tag="VF547_05790"
     CDS             complement(3644..5725)
                     /locus_tag="VF547_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8262369.1"
                     /translation="MAESAAKARNRQATPHSGYDWRRIAYLVQVSRAMDKLEEERLVP
                     ERKVLYQFSARGHDMAQAMLGSRLTHRRDATCGYYRSRPLLLSLGVPVEDALGSGMGR
                     EGGYSDGRDIGVVFNYPNPNGAPALPMSGGVGAQYTPTAGWAQAIRYYRTVLGDESYD
                     GAIAVVLGGDASCATGGFWSALTIATTQKLPMLFYVEDNGFGISVPSSFQTPGGDIAR
                     NLASFGNLAVFAGDGTDPAEAAELIERAVDQVRSGRGPALLRLAVPRLQGHSFQDTQA
                     YKSEELVRDEWSRDPLPKLKAHLVGALLTESEWDEIEARAQADVEQARQVAEARGVSD
                     PDRVLDHVFYEGEMQKLGGQWTHGYRAPRSHDEPRPEGQRINMVTAIRRTLDLELERN
                     PRVLLFGEDVGPKGGVHAVTLGLQEKYGPERVFDTSLNEEGIVGRAVGMALAGLMPVP
                     EIQFRKYAEPATEQINDCGTMRWRTANRFAAPMVLRIPGGFFKCGDPWHSQTNEVQFV
                     HNPGWQVAVPSNAEDAVGLLRTALRGNDPVVFFEHRAMLDDAWARRPWPGDDYVLPFG
                     RAKKVRQGDRITIVTWGAMVPRCEAAAADVSADVIDLRTLMPWDSETVLESVRRTRRC
                     LIVHEDLRTGGFGAEIAAVVADEAFLDLDAPVARVTMPDIPSPHNPVLLNWAVPGVEK
                     IRAKIEELVGF"
     gene            complement(5718..6926)
                     /locus_tag="VF547_05795"
     CDS             complement(5718..6926)
                     /locus_tag="VF547_05795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEX8262370.1"
                     /translation="MDDSFLLSDAPLLFDRLEEQKSDSLLQLIALCDADPRPDKIDVG
                     VGVYRDATGRTPILRAVKQAERRLWDGQETKAYLGSQGDARFTELIRPIVFGEAAARD
                     DRIVGLQTPGGCGALRLGADLVAKAEPKARILVGQPTWPNHGPLIQCAGVEMVPHPYY
                     DPASRRILFDEMVDALERARAGDLVLLHGCCHNPTGADLAADQWREVARIVERRGLVP
                     FVDLAYQGLGNGLEADAAGARLVVEAAEQAIVAQSCDKNFGVYRERVGNLFVKAASAR
                     AAKVAYGNLLQLSRTMWSMPPDHGAAVVRIVLEDRELRSDWDLELKEMCARIRTLRAK
                     LAAYDERLAYIDGQNGMFSMLPLTQAQVLELREEKGIYMANSGRFNVVGLSDGNVDSF
                     AAAVVEKMDG"
     gene            7050..7520
                     /locus_tag="VF547_05800"
     CDS             7050..7520
                     /locus_tag="VF547_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8262371.1"
                     /translation="MKEDDQLDGVDRRIVRELQRDAGLSHAALAERVGASAASVWRRV
                     RNLEKAGVLGRAVRIASPQALGRSVNVLCQVRMARQTVEARADFEDFIQSREEIVECY
                     AMSGEWDYLLRIAVRDVADYDRFIMRGVLAHPSVANAASNFALRQVKYTTEIPV"
     gene            complement(7521..>8247)
                     /locus_tag="VF547_05805"
     CDS             complement(7521..>8247)
                     /locus_tag="VF547_05805"
                     /inference="COORDINATES: protein motif:HMM:NF022633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HEX8262372.1"
                     /translation="ITHTYHTARDIVMERMARTKDELISSLYQHILRHQSADIQACRF
                     IFSLSEEKDDLSQWRGYAREGQGFTIGLSGPKIFKVAEPRDSPFSLMRIEYDHEKQRS
                     VLVRALEELEEELKLASQEEGADADQLANEAAMWFAWVADVRAMTNKHSSFRSEREWR
                     IVSVVSGDGEPNEIKVRSSGLRLVQYTELAFPAGDGKLPIVSIGIGPGFSGGEEVHAV
                     RSLCREVGYDPEIYFADTPYRRT"
BASE COUNT         1222 a         2926 c         2814 g         1285 t
ORIGIN      
        1 accatccggc cctcgcgcgc gaccaggacc tgggcgccgg cgagcagtcc cggggcaatg
       61 tagcgctcgt cgaggaagcg gtcgatccgg gcgagccgct ccggatcgaa ccccgcttcg
      121 gccgccgcca ctttttccat cggagctcct tcggctaatt cctcccctgc ctttgcaggg
      181 gagggggacc atgcgaagcc atgacggagg ggttcttgca gcgtccgaag aacccctcca
      241 ccgcgcttcg cgcggtcccc ctcccccgga aaaccggggg aggaattgaa cctgtcgcct
      301 agcgcttgag ccgagcccga atcaacgtca ttgcgagcgg agcgaagcaa tccaggctgc
      361 ggcgatttcg aacgggtaga ttgcttcgtc gctgcgctcc tcgcaatgac ggcggtggac
      421 gagaatcggg agaggcgggg tgatcgaggg cttcgagctg gatccggact ggccgcggat
      481 gagcctggcc gaggtcgacg ccctgctcac cgcgccgggc tcccgcctcg agacggccga
      541 ggccgtgatc cgcggcatcc cgacccgggt ctggaagaac gcgccgccca gcctcgcctt
      601 cctcgtccgc cattcgcgca cccacggcga tcggccgttc accgtctatg aggaggaacg
      661 gatcggcttc gaagcctcgt tccgcgccac cgccgcgctc gcccgcgagc tggtccggct
      721 gggagtcggc ccgggcgaca gggtcgcgat cgcgatggcc aacctgcccg aatggccggc
      781 ggccttcttc gccatcgtct cgctcggcgc gatcgcggtt ccgctcaacg cctggtggac
      841 cggggccgag ctcgaatacg ggctcgccga ttccggatcg aaggtgctga tctgcgacgg
      901 gccgcggtgg cagcgcatcg agccgcaccg ccccgccctg accgcgctcg agcatatcat
      961 cgtcagccgc ggcccggccc cggcgggcgg cgcccggctc gaggatctga tcggcgcgcc
     1021 gcccgcctgg gccgcccttc ccgactccga cctgcccgag gcggccgtcg cggccgacga
     1081 tccggcgacc atcctctaca ccagcggaac caccggccgg cccaagggcg cgctcggcac
     1141 ccaccgcaac ctgctcacca acatcctctc ggtcggctat tccaacgccc gcgcggcgct
     1201 gcgccgaggc gaggccccgc ccgagccggc ccccaaggtc gccctgatgg tgatcccgct
     1261 gttccactgc accgcgctga gcgccatgct gatggggacg atggtcgccg gccacacgat
     1321 cgtcttcatg cgcaaatggg atccgctcga ggcgatggcg atcatcgagc gcgagcgcgt
     1381 ccagctcacc ggcggcgtcc cgaccatcgc ctggcagata ttggagcatc cggagcgctc
     1441 ccgcttcgac ctgtcgagcc tcgagacgat cagctacggc ggcgctccgg cggcgcccga
     1501 gctggtcagg aggatctggg aggagttcgg agccctgccc ggcaacggct gggggatgac
     1561 cgagacgacc gcgaccgtga ccagccatgt cggcgaggat tatctcaacc gccccgacag
     1621 cgccggcccg ccggtgccgg tcgccgagct caggatcatg tcggcggacg gcgagcgcgc
     1681 gctgccggtc ggcgaaatcg gggaattgtg ggcgaaaggg ccgcagatcg tccagggcta
     1741 ctggaacaac ccggaggcga ccgcggcaac cttcgtcgac ggctgggtgc gcaccggcga
     1801 cctcgcccgg ctcgacgagg agggcttcct ctacatcgtc gaccgagcca aggacatggt
     1861 catacgcggc ggcgagaaca tctattcgat cgaggtcgag aacgtcctgt tcgaccaccc
     1921 cgcggtcacc gacgccgccc tcatcggcct cccccaccgg acgctcggcg aggagcccgc
     1981 ggcggtcgtc caccttgcgc ccggggcgac ggcgagcgag gaggaattga aggcctgggt
     2041 gcgcgagcgc ctcgccctgt tcaagacccc cgtgcggatc gccttcctgc cccagcccct
     2101 gccgaggaac gccaacggca agatcctgaa gcaggagctg aaggctctgt tcgactgatc
     2161 cgtcgcccca gcgcaggctg gggccccgtg gggtgaaagg cgacggggtt cttcaccccc
     2221 tgggggccca gccttcgctg cggcggcgcc gtatcctcaa tccgccttcc acccctccag
     2281 cacctcgacg aagcgggtca gccaggcgtt ggtctggtgg ccgtcgacga cccggtggtc
     2341 gatggtgagc gagacatagg ccatcgggcg gatctggatc gaatcggcgc cgtcgacctc
     2401 gcgcaccacc acccgcttct gaagcttgcc gacgcccagg atcgccgatt gcggctggtt
     2461 gatgatgatc ggcgccgcga acagcgagcc ggagacgccg tggttggaga tggtgaaggt
     2521 gccgcccgac acgtccttgc cggtgagctg ctcgcgccgc gccttctcga ccagctcgtc
     2581 gagcccggcg gcgatgccct tcagcgacag ggtctggacc ttgcgcagga cggggacgat
     2641 caggcccttc tcgccgagcg cggtgccgac gccgatgttg acgtcgtcga aaatctcgag
     2701 ccggtcctcg tgccagcggc tgttgatcgc cggcgcgacc ttcatcgcct cggccgcggc
     2761 ggcgacgata taggcgctgt aggtgagctt cacgcccttc ctcgcgaagg cctccttgtg
     2821 gcgctcgcga tgggcgacga ccgccccgaa atcggcctcg aacatggcgg tgacgtgcgg
     2881 cgcctcggtc accgaccgca gcatgttgcc ggcgatcttc agccgcatcc ggtcgtgcgg
     2941 cacgctgtgc gagcggatgc ccgtcccgag cgaggccgag ggatcgccga cctgcgccgg
     3001 gatgacgccc tcgaccgccc ggccggcctt ggccgactcc tcgacggcgc ggtcgacgtc
     3061 ggcgcgggtg atccggccgc cccggccggt gccctcgatc cgcgaggggt cgatgtcgtg
     3121 ctggacgagg gcccgtcgga ccgaggggga gaggcgatgc tcgcgatgcg cctcgttccc
     3181 cggcgaagac cggggcccag aggctccctt gctcggctgg gccccggcct tcgccggggt
     3241 gccggaaggc tcgtgctccg ccgcctccgc caccggcgcg atccggccga gcacggcgcc
     3301 cggcacggcc tcggcgtcgg tgtcgagcag gatctcgcga agcacccccg cggcgggcgc
     3361 gggcacctcc atcgccacct tgtcggtctc gagctcgacc agcgggtcgt tgacctcgac
     3421 ccggtcgccg acctgcctca gccaggcgcg caccaccgcc tgggtgccct cctgctcggc
     3481 ggggacgagc acgtcgatca tgacggcagg cccgtcaccc cggcggaggc cggggtccgc
     3541 tcccccgtca tgccggcgaa ggccggcatc cattccaagt cggcatggac cccagcctgc
     3601 gccggggtga cgatggccag gttcctccgg atccttgcca tcgtcagaag cccaccagct
     3661 cttcgatctt cgcccggatc ttctcgacgc ccggcaccgc ccagttgagg agcaccggat
     3721 tgtgcgggct ggggatgtcc ggcatcgtca cccgcgccac cggagcgtcg aggtcgagga
     3781 aggcctcgtc ggcgaccacc gcggcgatct cggcgccgaa gccgcccgtc ctcaggtcct
     3841 cgtggacgat caggcagcgg cgggtgcggc ggacgctttc gagcaccgtc tccgaatccc
     3901 acggcatcag cgtcctcagg tcgatgacgt cggccgagac gtcggcggcc gccgcctcgc
     3961 agcgcgggac catcgccccc caggtgacga tcgtgatccg atcgccctgg cggaccttct
     4021 tcgcccgacc gaacgggagc acatagtcgt cgcccggcca gggccgccgg gcccaggcgt
     4081 cgtcgagcat cgcccggtgc tcgaagaaga cgaccgggtc gttgccgcga agggcggtcc
     4141 ggagcagccc gaccgcgtcc tcggcgttgg acggcaccgc cacctgccag ccaggattgt
     4201 ggacgaactg gacctcgttg gtctggctgt gccagggatc gccgcacttg aagaagccgc
     4261 cgggaatcct cagcaccatc ggcgccgcga agcggttggc ggtccgccag cgcatcgtcc
     4321 cgcaatcgtt gatctgctcg gtcgccggct cggcatattt gcggaactgg atctcgggca
     4381 ccggcatcag cccggcgagc gccatgccga ccgcccggcc gacgatgccc tcctcgttga
     4441 ggctggtgtc gaacacgcgc tcgggcccgt atttctcctg gaggccgagg gtcaccgcat
     4501 ggaccccgcc cttgggcccg acatcctcgc cgaacaggag cacgcgcgga ttgcgctcga
     4561 gctcgaggtc gagcgtccgg cggatcgcgg tcaccatgtt gatccgctgg ccctcgggcc
     4621 ggggctcgtc gtggctgcgc ggggcccggt agccgtgagt ccattgcccg cccagcttct
     4681 gcatctcgcc ttcgtagaag acatggtcga gcacccggtc ggggtcggag actccgcgcg
     4741 cctcggcgac ctggcgggcc tgctcgacat cggcctgggc gcgggcctcg atctcgtccc
     4801 attccgactc ggtgagaagc gcgccgacga gatgcgcctt gagcttgggc agcggatcgc
     4861 ggctccactc gtcgcggacc agctcctccg acttgtaggc ctgggtgtcc tggaagctgt
     4921 ggccctgcag cctcggcacg gcgaggcgca gcagcgccgg cccgcggccg ctgcggacct
     4981 ggtcgaccgc gcgctcaatc agctccgccg cctcggcggg atcggtgccg tcgccggcga
     5041 agacggcgag gttgccgaag ctggcgaggt tcctggcgat gtcgccgccg ggcgtctgga
     5101 agctggacgg gaccgagatt ccgaagccgt tgtcctcgac gtagaagagc atcggcagct
     5161 tctgggtggt ggcgatggtc agcgccgacc agaagccgcc cgtcgcgcag gaggcgtcgc
     5221 cgcccagcac cacggcgatg gccccgtcgt agctctcgtc cccgaggacg gtccggtaat
     5281 agcggatcgc ctgcgcccag ccggcggtcg gcgtatattg cgctccgacc ccgcccgaca
     5341 tgggcagcgc cggcgccccg ttgggattgg gatagttgaa gaccacgccg atgtcgcggc
     5401 cgtccgaata gcccccttcc cgccccatgc ccgagccgag cgcgtcctcc accggcactc
     5461 cgagcgacag caggagcggg cgcgagcggt aatagccgca ggtggcgtcg cggcggtggg
     5521 tcaaccggct gccgagcatc gcctgggcca tgtcgtggcc gcgggcgctg aactggtaga
     5581 gcaccttgcg ctccgggacg agccgctcct cctcgagctt gtccatcgcc cggctgacct
     5641 gcacgagata ggcgatccgg cgccagtcat agccggaatg gggcgtggcc tggcggttcc
     5701 tggcctttgc cgcgctttca gccatccatc ttctccacca ccgcggcggc gaacgaatcg
     5761 acattgccgt cggagaggcc gacgacgttg aagcgcccgc tgttcgccat gtagatgccc
     5821 ttctcctcgc gcagctcgag cacctgggcc tgggtcagcg gcagcatcga gaacatgccg
     5881 ttctgcccgt cgatataggc cagccgctcg tcataggcgg caagcttcgc ccgcaaggtc
     5941 ctgattcggg cgcacatttc cttgagctcc aagtcccagt cggagcgcag ctcgcggtcc
     6001 tcgagaacga tccggaccac cgccgcgcca tgatcgggcg gcatcgacca catcgttcgc
     6061 gacagctgca ggaggttgcc gtaggcgacc ttcgccgcgc gggcgctcgc cgccttgacg
     6121 aacaggttgc cgacgcgttc gcgatagact ccgaaattct tgtcgcagct ctgggcgacg
     6181 atcgcctgct ccgccgcctc gacgaccagc cgggcgcccg ccgcgtcggc ctcgaggccg
     6241 ttgccgaggc cctgataggc gaggtcgacg aacggcacga gcccgcgccg ctcgacgatc
     6301 cgggccacct cgcgccactg gtcggcggcg aggtcggcgc cggtcggatt gtggcagcag
     6361 ccgtggagga ggacgaggtc gcccgcccgc gcccgctcca gcgcatcgac catctcgtcg
     6421 aacaggatcc ggcggctggc gggatcgtaa tagggatgcg gaaccatctc gacgccggcg
     6481 cactggatca ggggtccgtg attgggccaa gtcggctggc cgaccaggat ccgcgccttc
     6541 ggctcggcct tggcgacgag atcggcgccc agccggagcg cgccgcagcc gcccggcgtc
     6601 tgcaggccga cgatccggtc gtcccgggcc gcggcctcgc cgaacacgat cgggcggatc
     6661 agctcggtga agcgggcgtc gccctgcgag ccgagatagg ccttggtctc ctgcccgtcc
     6721 cacaggcgcc tctcggcctg cttgacggcg cgcaggatcg gcgttcggcc ggtggcgtcg
     6781 cgatagacgc cgacgccgac gtcgatcttg tccggccgcg gatcggcgtc gcacagcgcg
     6841 atgagctgca gcagcgagtc cgatttctgc tcctcgagcc ggtcgaacag cagcggcgcg
     6901 tcgctgagca agaaggaatc gtccatccac gtcctttccg ggcagccgag gcgcccagct
     6961 acatctaacc cacgccgctt ccaaatgcgt ctcagattga ccgagatatg gggtggaatt
     7021 gaaacgatcg ttcagttact gccgcagata tgaaagaaga tgatcagctt gatggggtcg
     7081 atcggcgaat cgtccgggag ctgcagcgcg acgcggggct gagccacgcc gcgctcgccg
     7141 aacgggtcgg cgcgtcggcc gcgtcggtgt ggcgccgggt gcgcaacctg gagaaggccg
     7201 gggtcctcgg ccgggcggtg cgaatcgcca gcccgcaggc gctcggccgt tcggtcaacg
     7261 tcctgtgcca ggtccggatg gcgcggcaga cggtcgaggc ccgcgccgat ttcgaggatt
     7321 tcatccagtc gcgcgaggag atcgtcgaat gctacgccat gtccggcgag tgggactatc
     7381 tgcttcgaat cgcggtgagg gacgtcgccg actacgaccg cttcatcatg cgcggagtgc
     7441 tcgcgcatcc cagcgtcgcc aacgcggcga gcaatttcgc cctgaggcag gtcaagtaca
     7501 cgaccgagat accggtctga tcaggtccgc cggtagggcg tgtcggcgaa atagatctcg
     7561 gggtcgtatc cgacctcgcg gcacagcgac cgcaccgcat ggacctcctc gccccccgag
     7621 aatccgggtc cgatgccgat gctgacgatc ggcagcttgc cgtcgccggc agggaatgcc
     7681 agctcggtat actggaccag ccgaagcccg ctgctgcgca ccttgatctc gttcggctcg
     7741 ccgtcgccgc tgaccaccga cacgatccgc cactcgcgct cggaccggaa ggagctgtgc
     7801 ttgttcgtca tcgcccggac gtcggcgacc caggcgaacc acatcgccgc ctcgttggcg
     7861 agctggtcgg cgtcggcgcc ctcctcctgg ctggcgagct tcagctcctc ctcgagctcc
     7921 tcgagcgccc ttaccaggac gcttcgctgc ttctcgtgat catattcgat cctcatcagg
     7981 ctgaacggcg aatcccgcgg ttcggcgacc ttgaagatct tcgggccgga gaggccgatc
     8041 gtgaagccct gcccctcgcg cgcatagcct cgccactggc tgaggtcgtc cttctcctcg
     8101 gagagcgaga agatgaaccg gcaggcctgg atgtcggcgc tctggtggcg caggatatgc
     8161 tggtagaggc tcgagatgag ctcgtccttc gtcctggcca tgcgctccat cacgatgtcg
     8221 cgggcggtat gataggtgtg ggtgatc
//
LOCUS       DASVQZ010000213         6259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_815305, whole genome shotgun sequence.
ACCESSION   DASVQZ010000213 DASVQZ010000000
VERSION     DASVQZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815305"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /gene="rutA"
                     /locus_tag="VF547_05810"
     CDS             <1..947
                     /gene="rutA"
                     /locus_tag="VF547_05810"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HEX8262373.1"
                     /translation="DFLLSMIKLRGFGGKTEFWEYNLESFTLMAGLAAVTEKIRIYAT
                     CPTLVIPPAFCARMCNTIDSISHGRFGLNLITGWQRPEYSQMGLWPGDEHFRNRYRML
                     GEYAHILRELWATGVSDFKGEYYRMEDCRVRPLPEGEMKIICAGSSDEGLAFSAQYAD
                     YAFCLGKGVNTPKAFASNNERLMAATAKTGRDVRIYALFMVIAAETDEEAMEKWISYR
                     EGVDEEAVAWLGAQGAADKTSATTNVRQLADRESAVNLNMGTLVGSYENVARMLDEAS
                     TVPNTGGVLLTFDDFIEGTEKFGTRIQPLMKCREGVGA"
     gene            complement(986..2242)
                     /locus_tag="VF547_05815"
     CDS             complement(986..2242)
                     /locus_tag="VF547_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262374.1"
                     /translation="MDSLRAFDEQPDYCAAEDDGGIEVPPEIGLDERRMHVRAYNYWV
                     SLLDGRPYPSIRDLAPDTLGDFGPNSVLLDFSDGADDPAVSWLGDALREECDLLGEVR
                     RISDVPKRSLLSRLTDHYMQIIANRAPIGFEAEFIGQRGKNTMYRGILMPFSSTGESI
                     DFIYGVINWKEIADQATASAIAEQASSALSAASQAQASPVWADGPNAEPLELDSPAER
                     TWDQPELDLDAGLADRLGVARDSADAARGADQRSRAALYRALGHAYDFALAAEADADG
                     YSELLEDAGLKAQARAPMTPVVKLVFGTGYDKARLTEFAAALSWARREGFPAGALRER
                     LEIDGLKALVRAERRARRPEPKPDGGQAAREALRAARPIGHVDLPGEAEFVLLLARRD
                     EVGGLAVVAPVADDALLERAIRKAAA"
     gene            2376..2451
                     /locus_tag="VF547_05820"
     tRNA            2376..2451
                     /locus_tag="VF547_05820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2409..2411,aa:Thr,seq:cgt)
     gene            complement(2477..3571)
                     /locus_tag="VF547_05825"
     CDS             complement(2477..3571)
                     /locus_tag="VF547_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262375.1"
                     /translation="MKLAYVLSCATLLAACVPTTEMRDGPDRERLVELVRRFCAAERS
                     EDRFDSHMLFVDSMRDMLARSAPFGDGRPLTSGDPKVGCEPGRTWYLGGSRLFAEVRL
                     ADRSDRLDLWRGDDWRIRDVLYGRKRRFGERKVGSLKAELVAEHGSEPPPVPPAPDVS
                     CLQPGHRFAFVGMDTTVFRQGSVVRLTPMVDRSPAGTAELPLRCTSGWAVTGPAALSA
                     DRKSLAIAPDAPVGAVVLVRFFHQGKPVSAQLRVVGKDEIVLTGRYTERSLEGCRAHE
                     PVRELEFRPDSRFSVTFMPFETYRDYWGRYSFDPATKRLRLEVEGGNFVPPHLDLDGG
                     AELVEGRLRLEGIFLGSRDGPPQSGCTYVF"
     gene            4026..6257
                     /locus_tag="VF547_05830"
     CDS             4026..6257
                     /locus_tag="VF547_05830"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8262376.1"
                     /translation="MRKFGQDNFASRFFADNEPVGAFGYGGHSRGLLASQAARQIGLD
                     STASSGSKSGVGFSTGSLTTPLTGSLILVTPDDAGNGIGLAPGNPVVTVGGASKIGTI
                     GTIGDQDFYKVTLEAGKTYEISMFGHVGGKSLVPLQDSYLELYSASGSLLASADGGAS
                     TLINTVNSGFDAILTFSVTVTGTYYINARAFDNDPTVGGSTGEGLGDYALAVKEVDPN
                     DPNVYHPYYDAGSPLYAFDWGSQVNKVNQSARNPDGNEGTRSTSANGADPTGNAQGTP
                     VYSSLLDIPALAAAQGKDITGKNVITIYFAKAGDLITSIEDPTAPGLPPVMLNVRDVQ
                     GFERDAVMTALGEFEKVADVVYLVVTDRAHADFTYTSYIGTPGPGISLLGSMSPPDEP
                     DEGLAQFNSGDERWNATNLAQGGFSFVTLIHEFGHGHGLAHPHDNGGHSGILNGVEPE
                     GAGVADYTTGDYDLNQGVFSMMSYEDGWQTSPYGNAPTNVGYGYLGGLMAFDIALIQD
                     KYGVNESYNTGNNTYTLKDVNAPGTFYSSIWDGGGTDEIVYSGSRDANIDLRQATLQY
                     EYGGGGFVSYAYGIFGGFTIANGVTIENATSGGGNDTLRGNAVGNRLDAGAGADVLEG
                     SGGADILIGGAGADTFRYASLSDSAGSAVDHITDFASGSDKIDLSGIDANSLLGGDQA
                     FSWIGSNAFGGSGAASAGELRAYQSGGNWFVEGDTNGDGNADLIIQVTVTSGSLTQTD
                     FLP"
BASE COUNT         1071 a         2147 c         2071 g          970 t
ORIGIN      
        1 tcgacttcct gctgtcgatg atcaagctca ggggcttcgg cggcaagacg gagttctggg
       61 aatataacct cgagagcttc accctgatgg ccggcctcgc cgccgtcacc gagaagatcc
      121 gcatctacgc gacctgcccg acgctcgtca tcccgcccgc cttctgcgcg cggatgtgca
      181 acacgatcga ttcgatcagc cacggccgct tcggcctcaa cctgatcacc ggctggcagc
      241 ggccggaata cagccagatg gggctctggc cgggcgacga gcatttccgc aaccgctacc
      301 ggatgctcgg cgaatatgcc catatcctgc gcgagctgtg ggcgaccggg gtctcggact
      361 tcaagggcga atattaccgg atggaggatt gccgggtccg ccctcttccc gagggcgaga
      421 tgaagatcat ctgcgccggc tcgtccgacg agggtctggc cttctcggcg caatatgccg
      481 attacgcctt ttgcctcggc aagggagtca acacgcccaa ggccttcgcc tcgaacaacg
      541 agcggctgat ggcggcgacg gcgaagaccg gccgcgacgt caggatctac gctttgttca
      601 tggtcatcgc cgccgagacc gacgaggagg cgatggagaa atggatctcc tatcgcgagg
      661 gggtggacga ggaggcggtg gcctggctcg gcgcccaggg ggcggccgac aagacctcgg
      721 ccacgaccaa cgtccgccag ctcgccgacc gggagagcgc ggtgaacctg aacatgggga
      781 ccctggtggg ctcctacgag aatgtggctc ggatgctcga cgaggcgagc acggtcccga
      841 acacgggcgg cgtgctgctc accttcgacg acttcatcga ggggaccgag aagttcggga
      901 cgaggatcca gccgctgatg aagtgccggg aaggcgtggg ggcttaactc atcctccccg
      961 ccgcgaagcg gtcgggagga tgaggttacg ccgcggcttt cctgatcgcc ctctcgagca
     1021 gcgcgtcgtc ggccaccggc gccaccacgg cgagtccgcc gacctcgtct cggcgggcga
     1081 ggaggaggac gaactcggcc tctccgggca ggtcgacatg gccgatcgga cgggcggcgc
     1141 gcagcgcctc gcgggccgcc tggcccccgt ccggcttcgg ctcgggacgg cgggcgcggc
     1201 gttctgcccg gaccagcgcc ttgaggccgt ctatctccag ccgctcgcgc agggctccgg
     1261 cggggaagcc ctcgcgccgc gcccagctca gggcggcggc gaactcggtg aggcgggcct
     1321 tgtcgtagcc ggtgccgaag acgagcttga ccaccggggt catcggggcg cgcgcctgcg
     1381 ctttcaggcc ggcgtcctcg agcagctccg aatagccgtc cgcatccgcc tcggcggcga
     1441 gagcgaagtc gtaggcgtgg ccgagggcgc gatagagggc ggctcggctg cgctggtcgg
     1501 cgccgcgggc ggcgtcggcg ctgtcgcggg cgaccccgag acgatcggcg aggccggcgt
     1561 cgaggtcgag ctcgggctgg tcccaggtcc gctcggcggg cgagtcgagc tcgagcggct
     1621 cggcattggg gccgtcggcc cagaccgggc tcgcctgggc ctgggaagcc gcgctcagcg
     1681 ccgacgaggc ctgctcggcg atggccgagg cggtggcctg gtcggcgatc tccttccagt
     1741 tgatcacgcc gtagatgaaa tcgatcgatt ccccggtcga ggagaagggc atcaggatgc
     1801 cgcgatacat ggtgttcttg ccgcgctgcc cgatgaactc ggcctcgaag ccgatcggcg
     1861 cccggttggc gatgatctgc atgtaatggt cggtgaggcg cgagaggagc gagcgcttgg
     1921 gaacgtcgct gatccgcctt acctcgccga gcaggtcgca ttcctcgcgc agcgcgtcgc
     1981 cgagccagga gacggccgga tcgtcggcgc cgtccgagaa atccagcagg acgctgttcg
     2041 gaccgaaatc gccgagcgtg tcgggcgcca ggtcgcggat cgagggatag ggccggccgt
     2101 cgagcagcga gacccagtaa ttataggccc tgacgtgcat ccggcgctcg tcgaggccga
     2161 tttccggcgg cacctcgatg ccgccgtcgt cctcggcagc gcaatagtcc ggctgctcgt
     2221 caaatgcccg caggctgtcc atcagcaggt cctcatgctc cccagtgggc ggaccctgca
     2281 ccggacgtgg ttgaaaaacc gttaagcttt ggaggccggg cacgtcgctt gtcatcgccg
     2341 cgcgccgctg atagagcgcg ccgctccccg ggagcgccgc tttagctcag atggtagagc
     2401 acatcattcg taatgatggg gtcgtgggtt cgagtccctc aagcggcacc acctccccgg
     2461 cgaccgggga tgctgctcaa aagacgtagg tgcagcccga ttgcggcggt ccgtcgcggc
     2521 tgcccaggaa gatgccctct aggcgcagcc ggccctcgac cagctcggcc ccgccgtcga
     2581 ggtcgaggtg gggcgggacg aaattgccgc cttcgacctc cagcctgagc cgcttcgtgg
     2641 ccggatcgaa gctgtagcgg ccccaatagt cccgataggt ctcgaacggc atgaaggtga
     2701 ccgagaagcg gctgtccggc cggaactcga gctcgcgcac gggctcgtgc gctcggcagc
     2761 cttcgaggct tcgctcggtg tagcgcccgg tgaggacgat ctcgtccttg ccgacgaccc
     2821 gcagctgggc cgagaccggc ttgccctggt ggaagaagcg aacgagaacg acggctccaa
     2881 ccggcgcatc gggcgcgatg gcgagcgact ttcgatccgc gctcagcgcc gcggggccgg
     2941 tgaccgccca gccgctcgtg catcgcaacg gaagctcggc ggttccggcc ggagaccggt
     3001 cgaccatcgg ggtcagccga acgaccgagc cctggcggaa gacggtcgtg tccatgccga
     3061 cgaaggcgaa ccgatggccg ggctggaggc aagagacgtc cggagccggg gggacgggcg
     3121 gcggctccga accgtgctcg gcgaccagct ccgccttgag gctgccgacc ttgcgctccc
     3181 cgaagcgccg cttgcggccg tagaggacgt cccggatccg ccagtcgtcg ccgcgccaca
     3241 ggtccagcct gtcgcttcga tcggcgaggc ggacctcggc gaaaagccgc gacccgccca
     3301 ggtaccaggt ccggccgggc tcgcatccca ccttcgggtc gcccgaggtc agcggcctgc
     3361 cgtcgccgaa gggagcgctt cgggcgagca tgtcccgcat cgagtcgacg aacagcatgt
     3421 gcgaatcgaa gcggtcctcg cttcgctccg cggcgcagaa tcgccggacc agctcgacga
     3481 gccgctcccg gtcggggccg tcgcgcatct ccgtcgtcgg gacgcaggcg gccagcagcg
     3541 tggcacagga caagacatag gcaagcttca tcgaccgttc ctccggaacg ccccaggctg
     3601 gagccgatcg ccgccgcgat caacccgtcg cccgcgccgc cgcggagccc gccgaagcgc
     3661 gccgcgtggg atcaagaaac atccaaaacg tgagcaatat tacgtagtta cgacaggttc
     3721 ccttaaattc gagaacccaa taagtctcgg cgaagctgac aggtttttca cgcttgaagc
     3781 tgttcgaggg gcgaagatgg gggctcgacc gtacgttgcg accgacgctg cttctgcgct
     3841 ccggcaggcg tttcatccac accatcggcc gttcccggcg ctggccaatc cattttccga
     3901 ccgaccagcc ccgggcttga tgacgctcaa caggccagac ggcgcagcac cctattgcgg
     3961 gcccgcctcg taagtcaccg taagtcactc acctaactca acatcatcga tgcaggagac
     4021 aagggatgcg taaattcgga caggacaact ttgcttcgcg cttcttcgcc gacaacgagc
     4081 cggtcggcgc gttcggctac ggcggccatt cgcgcgggct cctcgcctcg caggcggcgc
     4141 ggcagatcgg cctcgactcg acggcgtcga gcggctccaa gtcgggagtg ggcttcagca
     4201 ccgggagcct gacgacgccc ctcaccggca gcctcattct cgtcacgccc gacgatgccg
     4261 gcaacggcat tgggctggcc ccgggcaatc cggtcgtcac cgtcggcggc gcctcgaaga
     4321 tcggcaccat cggcacgatc ggcgaccagg atttctacaa ggtcacgctc gaggccggca
     4381 agacctacga gatcagcatg ttcggccatg tcgggggcaa gtcgctcgtc ccgctccagg
     4441 attcctacct cgagctctac agcgcctcgg gtagcttgct ggcttcggcg gacggcggcg
     4501 ccagcactct gatcaacacc gtcaattccg gcttcgacgc gatcctgacc ttctcggtca
     4561 ccgtcaccgg aacctactat atcaacgccc gcgccttcga caacgatccg acggtcggcg
     4621 gctcgaccgg cgaaggcctc ggcgactacg ccctggccgt gaaggaggtc gatcccaacg
     4681 acccgaacgt ctaccacccc tattacgacg ccggcagccc gctctacgca ttcgactggg
     4741 gctcgcaggt caacaaggtg aaccagagcg cccgcaaccc ggacggcaat gagggcaccc
     4801 gctcgacctc ggccaacggc gccgacccca ccggcaacgc gcagggcacg ccggtctatt
     4861 cgtcgctgct cgacattccg gcgctcgccg cggcgcaggg caaggacatc accggcaaga
     4921 acgtgatcac catctacttc gccaaggccg gcgacctgat cacctcgatc gaggatccga
     4981 ccgcgcccgg gctgcccccg gtgatgttga acgtccgcga cgtccagggc ttcgagcgcg
     5041 acgccgtgat gaccgcgctc ggcgagttcg agaaggtggc cgacgtcgtc tatctcgtcg
     5101 tcaccgaccg cgcccatgcc gatttcacct acacgagcta catcggcact ccggggccgg
     5161 gcatttcgct gctcggctcg atgagcccgc cggacgagcc cgacgagggc ctggcccagt
     5221 tcaactccgg cgacgaaagg tggaacgcga ccaacctggc ccagggcggc ttctcgttcg
     5281 tcaccctgat ccacgagttc ggccacggcc acggcctcgc ccatccgcat gacaatggcg
     5341 gccattcggg catcctgaac ggggtcgagc ccgagggcgc cggagtcgcg gactacacga
     5401 ccggcgacta cgacctcaac cagggcgtct tctcgatgat gtcctacgag gacggctggc
     5461 agacctcgcc ctacggcaat gcgccgacca acgtcggcta tggctatctc ggcgggctga
     5521 tggccttcga catcgccctc atccaggaca aatatggagt caacgaaagc tacaataccg
     5581 gcaacaacac ctacaccctg aaggacgtca acgccccggg caccttctat tcgtcgatct
     5641 gggacggcgg cggaaccgac gagatcgtct acagcggctc gcgcgacgcc aatatcgacc
     5701 ttcgccaggc caccctccag tatgagtatg gcggcggcgg cttcgtctcc tacgcctacg
     5761 gcatcttcgg cggcttcacc atcgccaacg gggtgacgat cgagaatgcg accagcggcg
     5821 gcggcaacga cacgcttcgc ggcaacgcgg tcggcaaccg gctcgacgcc ggcgccggcg
     5881 cggacgtcct cgagggttcg ggcggagccg atatcctcat cggcggcgcc ggcgccgaca
     5941 cgttccgtta cgcctcgctg tcggattccg ccggcagcgc ggtcgatcac atcaccgact
     6001 tcgcgtccgg atcggacaag atcgaccttt ccgggatcga cgccaacagc ctcctcgggg
     6061 gcgaccaggc cttcagctgg atcggctcga acgcgttcgg cggctccggc gccgcctccg
     6121 cgggagaatt gcgtgcctac cagtccggcg gcaattggtt cgtcgaaggc gacaccaacg
     6181 gcgacggcaa tgccgacctg atcatccagg tcaccgtcac cagcggttcg ctcacccaga
     6241 ccgacttcct gccttgatc
//
LOCUS       DASVQZ010000214         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_815513, whole genome shotgun sequence.
ACCESSION   DASVQZ010000214 DASVQZ010000000
VERSION     DASVQZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815513"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VF547_05835"
     CDS             <1..1518
                     /locus_tag="VF547_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX8262377.1"
                     /translation="WIVFDRRGIKVAPVDDSLVMSFDLDAGGLNSHSMDDLAKKHLDH
                     ECIAYKDVCGSGQKQIKFNQVPLDRATEYAAEDADVTLRLWTRFKNRLPHERVQRVYE
                     LVDRPLVPVVARMEREGVKVDREELNKLSQEFQTQIAALEERICGEAGCQFTIGSPQQ
                     LGEVLFGKMGLKGGRKGKSGTWSTDVNELERLSRDGVPIARLVLEWRQLSKLKSTYTD
                     ALQQQINKETGRVHTSYSLSGAQTGRLSSTDPNLQNIPIRTEIGRRIRDAFVAEAGHV
                     ILAADYSQIELRLAAHMADVPQLREAFARGDDIHSLTAQELFGEVNRDTRASAKTINF
                     AILYGISRWGLAGRLSITADEAQAMIDRYFERFPGIQNYIADTLSKVRETGFTTTLFG
                     RKTHFPMIRSRVQHERQGAERAAINAPIQGTSADIIKRAMVRMCPALEREGLGGVRML
                     MQVHDELVFEVPEADVERAAAVIRSVMERAAEPAAALSVPLGVDIGTGPNWGAAH"
     gene            1515..3098
                     /locus_tag="VF547_05840"
     CDS             1515..3098
                     /locus_tag="VF547_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX8262378.1"
                     /translation="MTPAPPGAGEDIAALAKGGRTNFFGFLIRLAARLPFLFIAGRLY
                     GPEALGAFAYAVIVMEFAAQLSTLGLKRGLAQQLSTTEQPHSCVVMDAILAAFIASAV
                     STLILMLFPQAMFPAGGVHGADWLLPMVIFGIAGADIALAALAYRHDIGATVRARAIV
                     EPWTISLAAAACVLIFPAAEDGTRSRDGLIIAYVISVTAGLAASLWPLYRSYGLPPHW
                     KPHPAHLFYVARQNIPLAAADAIEWGSRRLDIAILGRFVAPYWVGVYYVGQQVASLPQ
                     KLKTSFDPILAPVITQKLAEGDKQAVAKQVRQVGFWVIAAQAGIALALGIPGEAVMGM
                     VGAAFVGGTGALAFLLAAEVVAATAAVSEAALVYVARHRNLMISALMIAVQAALTVVF
                     ILAVERLPLRPGSVQAYQAASAAAALMVALGLASILKARLLCRLLDAPVQGWRWPLVW
                     AGAAAVVVGQAFTMLPQSLEWVELSIGIPAIMATFGIVVWLKGFTHDDRVLFRLKKGE
                     EPALPPPPGTAPGADPPPA"
     gene            complement(3095..>4179)
                     /gene="purH"
                     /locus_tag="VF547_05845"
     CDS             complement(3095..>4179)
                     /gene="purH"
                     /locus_tag="VF547_05845"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX8262379.1"
                     /translation="DFRKYLAAKAFAATAAYDSAIAQWFAFADQGQMFPATLPLTLKL
                     GGELRYGENPHQKASLYLPAGPHGRGVAQAEQLQGKALSYNNYNDADAALELAAEFRD
                     SGPACVIVKHANPCGVATGASLVEAYEAAFACDTVSAFGGIIAVNRPIDEAAAEAMTR
                     IFTEVVIAPDADAAARAVFARKKNLRLLLTGDLPDPGRPGLVLKSIAGGYLLQSRDGA
                     RLAPEPLQVVTRRAPSERELADCRFAWTVAKHVKSNAIVYARDGATAGIGAGQMNRLE
                     SARIAAWKARDAAEKAKWPEPRTIGSAVASDAFFPFADGLLAAVEAGATAVIQPGGSI
                     RDDEVIAAADEAGLAMIFTNVRHFRH"
BASE COUNT          617 a         1490 c         1430 g          642 t
ORIGIN      
        1 tggatcgtct tcgaccgccg cggaatcaag gtcgcgccgg tggacgacag cctggtgatg
       61 agcttcgacc tcgacgccgg cggcctcaac agccattcga tggacgatct ggcgaagaag
      121 catctcgacc acgagtgcat cgcctacaag gacgtctgcg gctcgggcca gaagcagatc
      181 aagttcaacc aggtccccct cgaccgggcc accgaatatg cggccgagga cgccgacgtg
      241 accttgcggc tgtggacccg gttcaagaac cggctgccgc acgagcgcgt ccagcgcgtc
      301 tacgagctgg tcgaccggcc gctggtgccc gtcgtcgccc ggatggagcg cgagggggtc
      361 aaggtcgacc gcgaagagct gaacaagctc agccaggagt tccagaccca gatcgccgcc
      421 ctcgaggagc ggatctgcgg cgaggcgggc tgccagttca ccatcggcag cccccagcag
      481 ctgggcgagg tgctgttcgg caagatgggc ctgaagggcg gccgcaaggg caagtccggc
      541 acctggtcga ccgacgtcaa cgagctcgag cggctgtcgc gcgacggcgt tccgatcgcc
      601 cgcctggtgc tcgagtggcg ccagctctcc aagctcaagt cgacctatac cgacgcgctc
      661 cagcagcaga tcaacaagga gaccggccgg gtccacacca gctattcgct gtccggcgcc
      721 cagaccggcc gcctgtcgtc gaccgatccg aacctccaga acatcccgat ccgcaccgag
      781 atcgggcggc ggatccgcga cgcgttcgtc gccgaagccg gccacgtcat cctcgccgcg
      841 gactacagcc agatcgagct tcgcctcgcc gcccacatgg ccgacgtgcc gcaactgcgc
      901 gaggctttcg cccgcggcga cgacatccac agcctcaccg cccaggagct gttcggagag
      961 gtcaaccgcg acacccgcgc cagcgccaag acgatcaatt tcgccatcct ctacggcatc
     1021 tcgcgctggg gcctcgccgg ccgcctgtcg atcacggccg acgaggcgca ggcgatgatc
     1081 gaccgctatt tcgagcgctt ccccggaatc cagaattata tcgccgacac gctttccaag
     1141 gtgcgcgaga ccggcttcac caccaccctg ttcggccgaa agacccattt cccgatgatc
     1201 aggagccgag tccagcacga gcgacagggg gccgagcgcg ccgcgatcaa cgccccgatc
     1261 cagggcacga gcgccgacat catcaagcgg gccatggtcc gcatgtgccc ggcgctcgag
     1321 cgcgaaggcc tgggcggggt ccggatgctg atgcaggtcc atgacgagct cgtcttcgag
     1381 gtgccggagg ccgacgtcga gcgcgccgcc gccgtcatcc ggagcgtcat ggagcgcgcc
     1441 gccgagccgg cggccgcgct gagcgtccca ctcggtgtcg acatcggcac cggaccgaac
     1501 tggggcgccg cccattgacc ccggccccgc ccggggcggg cgaagacatc gccgccctcg
     1561 ccaagggcgg gaggaccaac ttcttcggat tcctgatccg gctcgccgcg cgcctgccct
     1621 tcctgttcat cgcgggacgg ctctacggac cggaagcgct cggcgccttc gcctacgccg
     1681 tgatcgtcat ggagttcgcg gcccagctct ccaccctcgg cctcaagcgt gggctggcgc
     1741 agcagctgtc gacgaccgag cagccgcaca gctgcgtggt gatggacgcg atcctcgcgg
     1801 ccttcatcgc ctcggccgtc tcgactttga tcctgatgct gttcccccag gccatgtttc
     1861 cggccggcgg cgtccacggc gccgactggc tgctgccgat ggtgatcttc gggatcgcgg
     1921 gggccgacat cgccttggcg gcgctcgcct atcgccacga catcggcgcg acggtgcgcg
     1981 cccgagcgat cgtcgagccg tggacgatca gcctcgccgc cgcggcctgc gtcctcatct
     2041 tccccgccgc ggaggacggc acgcgctcac gcgacgggct gatcatcgcc tatgtgatct
     2101 cggtgacggc gggcctcgcc gcatcccttt ggccgctcta ccgcagctac ggcctgcccc
     2161 cgcactggaa gccgcatccg gcacacctct tctacgtcgc ccgccagaac atcccgctcg
     2221 ccgccgccga cgcgatcgag tggggctcgc gccggctcga catcgccatc ctcggccggt
     2281 tcgtcgcgcc ttactgggtc ggcgtctatt atgtcggcca gcaggtcgcc tcgctcccgc
     2341 agaagctgaa gaccagcttc gacccgatcc tcgccccggt catcacccag aagctggccg
     2401 agggcgacaa gcaggcggtt gccaagcagg tgcggcaggt cggcttctgg gtgatcgccg
     2461 cccaggccgg tatcgcgctc gccctcggca ttccgggcga ggcggtgatg gggatggtcg
     2521 gagcggcgtt cgtcggcggc accggggcgc tcgccttcct tctcgccgcc gaggtcgtgg
     2581 ccgccaccgc cgcggtctcg gaggcggccc tggtctacgt cgcgcggcac cgcaacctga
     2641 tgatctcggc cctgatgatc gccgtccagg cggcgctcac cgtcgtcttc atcctcgccg
     2701 tcgagcggct gccgctgcgt ccgggaagcg tccaggccta tcaggcggcc agcgccgcgg
     2761 cggccttgat ggtggcgctc gggcttgcct ccatcctcaa ggcgcgcctg ctctgccgcc
     2821 tcctcgacgc gccggttcag ggctggcggt ggccgctggt ctgggccgga gccgccgcgg
     2881 tggtggtggg ccaggccttc accatgctgc cccagtcgct cgaatgggtc gagctttcga
     2941 tcggcatccc ggcgatcatg gcgacgttcg ggatcgtcgt ctggctgaag ggcttcaccc
     3001 acgacgaccg cgtcctcttc cgcctgaaga aaggcgagga gcccgctttg ccgccgccgc
     3061 ccggcaccgc tccgggagcc gacccgccgc ccgcctagtg gcggaagtgc cggacgttgg
     3121 tgaagatcat ggccagcccg gcctcgtcgg ccgcggcgat gacctcgtcg tcgcggatcg
     3181 agccgccggg ctggatcacc gccgtcgccc ccgcctccac cgccgcgagc aggccgtccg
     3241 cgaacgggaa gaaggcgtcc gaggcgacgg ccgagccgat cgtccggggt tccggccact
     3301 tggctttctc ggcggcgtcg cgcgccttcc aagcggcgat gcgggcggat tcgaggcggt
     3361 tcatctggcc cgcgcctatc ccggccgtgg ccccgtccct ggcgtagacg atggcgttgg
     3421 acttgacgtg cttggcgacc gtccaggcga agcggcagtc ggcaagctcg cgctcgctcg
     3481 gagcgcgccg ggtgacgacc tggagaggct cgggggcgag ccgggcgccg tcgcgcgact
     3541 ggagcagata gccgccggcg atcgatttca ggaccagccc gggccggccc ggatcgggca
     3601 gatcgccggt caggaggagc cgcaggttct tcttgcgggc gaagacggcc cgagcggcgg
     3661 cgtcggcatc gggggcgatc accacctcgg tgaagatccg ggtcatcgct tcggccgccg
     3721 cctcgtcgat cggccggttg acggcgatga tgccgccgaa ggccgagacg gtgtcgcagg
     3781 cgaaggcggc ctcataggcc tcgaccaggc tcgccccggt ggcgactccg cagggattgg
     3841 cgtgcttgac gatcacgcag gcggggccgg aatcgcggaa ctcggcagcc agctcgagcg
     3901 cggcgtcggc gtcgttgtaa ttgttgtagc tcagcgcctt gccctggagc tgctcggcct
     3961 gggcgacgcc tcggccgtga ggcccggcgg gaaggtagag cgaggccttc tggtggggat
     4021 tctcgccgta gcgcagctcg ccgccgagct tcagggtgag gggcagggtt gcggggaaca
     4081 tctggccctg atcggcgaag gcgaaccatt gggcgatcgc cgaatcatag gccgccgtcg
     4141 cggcgaaagc ctttgccgcc agatacttgc ggaagtcga
//
LOCUS       DASVQZ010000215         5839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_525822, whole genome shotgun sequence.
ACCESSION   DASVQZ010000215 DASVQZ010000000
VERSION     DASVQZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525822"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VF547_05850"
     CDS             <1..575
                     /locus_tag="VF547_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262380.1"
                     /translation="NLSETGAMLEGAGLPDAGMKLILARGDLHVAATVAWAAGPRRGV
                     RFDGPTPVHEWTGGAKPRPLECTGLRDQRRVDAIQAEARADLASGRALRAAESTPPPP
                     PPAANPDLDSKLAHELGYVQRLLENLGDELIKDLFLVQRHGQSLQSLDLVGQILGHVA
                     AILMAEDKAAMVDQIGMEDLRARLKRKPIG"
     gene            complement(785..1363)
                     /locus_tag="VF547_05855"
     CDS             complement(785..1363)
                     /locus_tag="VF547_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX8262381.1"
                     /translation="MRQIRPFLALAAALAVSGCLSFGAKPPPTLMRLTSDAQRPAASR
                     TAAAGQAITVVPPSAPAELMTPRLPVRTGATQVAYVKDAQWVEVPTILFARLLSETIA
                     ARTGRVVLDPKQFTFDPGQRLTGTLQAFGLDEDRMEAVLVYDAAIARGEAVETRRFEA
                     RVPVPALDAASAPAALNQVANQVAAEVAAWVG"
     gene            complement(1360..2349)
                     /locus_tag="VF547_05860"
     CDS             complement(1360..2349)
                     /locus_tag="VF547_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8262382.1"
                     /translation="METRSNHILVGSVVMGALAAVLLFIVWLSNAGGDQDRRFDILFR
                     QAVDGLAKGSAVTFSGVPVGQVESINLMPQTPEFVRVRITVDEKTPVLVGTTATIKGV
                     GFTGVSQIQLDPADNDPRRRRNARPIECPQTNPEAQCPFGVPIIPTKPGALGQVLESA
                     PELLNRVSTLTARLTELLGDRNQASIAAILENVQVISRNLAERSDEIAATLAEARIAI
                     RQTGAAVEQMGQLANTTDQVLARDVRPMIGDLRQTIRAAETSMNNLDAAIGDARPGLQ
                     AFSKQTIPEVGQLVRDLREMSDSLSAVSQRLNQQGAGGIIGGQKLPDYKPRKR"
     gene            complement(2349..3173)
                     /locus_tag="VF547_05865"
     CDS             complement(2349..3173)
                     /locus_tag="VF547_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8262383.1"
                     /translation="MNGEADIVIEVRGLRNSFGEAVIHDGLDLEVRRGEILGVVGGSG
                     TGKSVMMRSIIGLQRPDEGEVRVFGEPMLDREDSALQPIRKRWGILFQGAALFSTLTV
                     AENVQVPLREFYPRLSERLLDEIAAYKIVMVGLPEDAGPKFPAELSGGMRKRAGIARA
                     LALDPELLFLDEPTAGLDPIGASAFDQLVLNLRDRLGLTVFLITHDLDTLYAICDRVA
                     VLADRKVIAVGTIPELLALDHEWIQEYFRGPRGRAAEEAVEEAEARSANTDGGGES"
     gene            complement(3170..4279)
                     /locus_tag="VF547_05870"
     CDS             complement(3170..4279)
                     /locus_tag="VF547_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8262384.1"
                     /translation="MSTVARFTETSENGRRVLRFHGDLTLSRLGVLPTRLERLKGDNF
                     LIDLSDVGRMDTIGAWIVHRTARDRHGDIVGADEGTATLLRQVESADRPVKVRPDPPS
                     PISRVLTQVGAATLDAGRTIFGMIAFLGAMLISIGHVIRQPRRFRLNAVVQRFEVVGV
                     HAFGIIGLMSFLVGIVIAQQGAVQLRQFGAEIYTINLIGRSSIKELGVLMTAIMVAGR
                     SGSSFAAQLGSMKLAEEVDAMRTIGVSPMEALVLPRVFATVLMMPLLSFYAALIAIIG
                     GGIFCWISLEIPPVTFVQRIREVVPLTDLWQLLIKAPVFGLIIAMAGCFQGMQVKADA
                     EEVGFRTTTAVVHAIFMVIVLDAFFAVFFTEIGWL"
     gene            4335..5033
                     /locus_tag="VF547_05875"
     CDS             4335..5033
                     /locus_tag="VF547_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX8262385.1"
                     /translation="MLHADSMKARLAIYDMDRTITRRATYTPFLIHAALRLAPWRLAL
                     LPLVLLAMGAYVLKLIDRGRLKEINYGLLVGRAVALGRLEPVIESFADAQLAGNIMPG
                     ARRAIAADKAAGRRLVMATASYRLYAAAIAKRLGFDDVVATETGLDTEGRVVAKIDGT
                     NCYGPAKKDMILAWLQREGLEREAVHIRFYSDHVSDHHVHRWADEAVAANAHARLVRL
                     ARAEGWEVVDWSDR"
     gene            complement(5183..5587)
                     /locus_tag="VF547_05880"
                     /pseudo
     CDS             complement(5183..5587)
                     /locus_tag="VF547_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
     gene            complement(5687..>5839)
                     /locus_tag="VF547_05885"
     CDS             complement(5687..>5839)
                     /locus_tag="VF547_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX8262386.1"
                     /translation="ASAPAAMTHLSVPDERKQALGITDNLVRVSIGVEDPDDLIADFD
                     QALGAV"
BASE COUNT          919 a         1937 c         2093 g          890 t
ORIGIN      
        1 gcaacctctc ggaaaccggc gcgatgctgg agggcgcggg ccttcccgac gccgggatga
       61 agctgatcct ggcgcgcggc gacctgcacg tcgccgcgac cgtcgcctgg gcggcgggac
      121 cccgccgcgg agttcggttc gacggcccga cgccggtcca tgagtggacc ggcggcgcca
      181 agccgaggcc gctcgaatgc accgggctgc gcgaccagcg tcgggtcgat gcgatccagg
      241 ccgaggcgcg cgccgacctg gcgtcgggcc gggcgctgcg cgccgcggag tcgacgccgc
      301 cccccccgcc gccggcggcg aaccccgacc tcgattcgaa gctggcgcac gagctcggct
      361 atgtgcagcg gctgctcgag aatctcggcg acgagctgat caaggacctg ttcctcgtcc
      421 agcgccacgg ccagtcgctg cagagcctcg atctggtcgg ccagatcctg ggccatgtcg
      481 ccgccatcct gatggccgag gacaaggcgg cgatggtcga ccagatcgga atggaggacc
      541 tgcgggcgcg gctgaagagg aagccgatcg gctaggccgc cgagggatca ggctgaatcg
      601 ctgccacccg ttcgccctga gcctgtcgaa gggctttctt ctcgttcgtg aaggaaaggg
      661 aaggcttcga caagctcagc ccgaacggat gggtaggtcg aggactgtct ttcgatccgg
      721 cggttcgact ttgctcagcg gctacctgga tcagcggcaa cgcgattaat cgaacctcga
      781 cggcctaccc cacccaggcg gcgacctccg ccgcgacctg attggccacc tggttgagcg
      841 cggccggcgc ggaggcggcg tcgagggccg ggaccggcac ccgcgcctcg aagcgacggg
      901 tctcgaccgc ctcgccgcgc gcgatggcgg cgtcgtagac cagcaccgcc tccatccggt
      961 cctcgtcgag gccgaaggcc tggagggtgc cggtgagccg ctggccgggg tcgaaggtga
     1021 attgcttggg gtcgagcacg acccggcccg tccgcgccgc gatcgtctcc gacagcaggc
     1081 gggcgaacag gatggtcggg acctcgaccc attgcgcgtc cttgacgtag gcgacctggg
     1141 tcgcgccggt gcgcaccggc aggcgcgggg tcatcagctc ggcgggcgcg ctcggcggga
     1201 cgacggtgat cgcctggccg gccgcggcgg tgcggctggc cgcggggcgc tgcgcgtccg
     1261 aggtgagccg catgagggtg ggcggcggct tggcgccgaa gctgaggcag ccggagacgg
     1321 ccagcgcggc ggcgagggcg aggaacgggc ggatctgtct catcttttcc tcggcttgta
     1381 gtcgggcagc ttctggccgc cgatgatccc gccggcgccc tgctggttga ggcgctggga
     1441 gaccgcggag agcgaatcgg acatctccct caggtcgcgg accaattgcc cgacctcggg
     1501 gatggtctgc ttcgagaagg cctgaagccc gggccgggca tcgccgatcg cggcgtcgag
     1561 attgttcatg ctggtctcgg cggcgcggat ggtctgcctg aggtcgccga tcatcggccg
     1621 gacgtcgcgc gccagcacct ggtcggtcgt gttggcgagc tgccccatct gctcgacggc
     1681 ggcgccggtc tggcggatcg cgatccgcgc ctcggccagg gtggcggcga tctcgtccga
     1741 tcgctcggcg aggttgcggc tgatgacctg aacgttctcg aggatggcgg cgatcgacgc
     1801 ctggttgcgg tcgccgagca gctcggtgag gcgcgcagtg agggtcgaca cccggttgag
     1861 cagctcggga gcgctctcca gcacctggcc gagagcgccg ggcttggtcg ggatgatcgg
     1921 cacgccgaac gggcattgcg cttcgggatt ggtctgcggg cattcgatcg gccgcgcgtt
     1981 gcgccggcgg cgcgggtcgt tgtcggccgg atcgagctgg atctgcgaga ctccggtgaa
     2041 gccgacgccc ttgatggtcg ccgtggtccc gaccagcacc ggcgtcttct cgtcgacggt
     2101 gatccggacg cggacgaact cgggcgtctg cggcatcagg ttgatcgatt cgacctggcc
     2161 gaccgggacg cccgagaagg tcaccgcgct gcccttggcg aggccgtcca ccgcctggcg
     2221 gaacaggatg tcgaagcgcc ggtcctggtc gccgccggcg ttcgacagcc agacgatgaa
     2281 cagcaggacg gcggcgagcg cgcccatgac gacgctgccg acgagaatat ggttcgaccg
     2341 ggtttccatc agctctcacc gcccccgtcg gtgttggcgg acctcgcttc cgcttcttcg
     2401 acggcctcct cggccgcgcg gccgcggggt ccgcggaaat attcctgtat ccattcgtgg
     2461 tccaaagcca gcagttccgg aatcgttccg accgcaatca ccttgcggtc ggcgagcacg
     2521 gcgacacggt cgcagatggc gtagagcgtg tcgagatcat gggtgatcag gaagaccgtc
     2581 aggccgagcc ggtcgcggag gttgaggacc agctggtcga aggccgaagc gccgatcggg
     2641 tccagccccg cggtgggttc gtcgaggaac aggagctcgg gatcgagcgc gagcgcccgg
     2701 gcgattccgg cgcgcttgcg catgcccccc gaaagctccg cggggaattt cgggcccgca
     2761 tcctcgggca gcccgaccat gacgatcttg taggcggcga tctcgtcgag cagccgctcg
     2821 gacaggcgcg gatagaattc gcgcagcggc acctggacgt tctcggccac ggtgagggtc
     2881 gagaacaggg ccgcgccctg gaagaggatg ccccagcgct tgcggatcgg ctgcagcgcc
     2941 gaatcctcgc ggtcgagcat cggttcgccg aagacgcgga cctcgccctc gtcggggcgc
     3001 tgcagcccga tgatcgagcg catcatcacc gacttgccgg tgccggagcc gccgacgact
     3061 ccgaggatct cgccgcgccg cacctcgagg tcgagcccgt cgtggatgac ggcctcgccg
     3121 aagctgttcc tgaggccgcg cacctcgatg acgatgtccg cttcgccgtt cacagccagc
     3181 ctatctcggt gaagaagacc gcgaagaagg cgtcgagcac gatcaccatg aagatggcgt
     3241 ggacgacggc cgtggtggtc cggaagccga cctcctcggc gtcggccttg acctgcatgc
     3301 cctggaagca gccggccatg gcgatgatga ggccgaacac cggcgccttg atcagcagct
     3361 gccacaggtc ggtcagaggc accacctcgc ggatccgctg gacgaaggtg accggcggga
     3421 tttcgagcga gatccagcag aagatgccgc cgccgatgat cgcgatgaga gcagcataga
     3481 agctcagcag cggcatcatc agcaccgtcg cgaagacccg cggcagcacc agcgcctcca
     3541 tcggcgacac gccgatggtg cgcatcgcgt ccacctcctc ggcgagcttc atcgagccca
     3601 attgcgcggc gaaggaggaa ccggagcggc cggcgaccat gatcgccgtc atcagcacgc
     3661 ccaattcctt gatcgacgag cggccgatga ggttgatcgt gtagatttcg gcgccgaact
     3721 gcctgagctg gaccgcaccc tgctgggcga tgacgatgcc gacgaggaag ctcatcagcc
     3781 cgatgatgcc gaaggcgtgg actccgacca cctcgaagcg ctggacgacg gcgttgaggc
     3841 ggaagcgccg gggctgccgg atgacgtggc cgatcgagat cagcatcgct ccgaggaagg
     3901 cgatcatgcc gaaaatggtg cggccggcat cgagcgtcgc cgccccgacc tgggtaagga
     3961 cccgggagat gggcgagggc gggtcgggtc ggaccttgac cgggcggtcg gcgctctcga
     4021 cctggcgaag cagggtcgcc gtgccctcgt cggcgccgac gatgtcgccg tggcggtcgc
     4081 gcgcggtccg gtggacgatc cacgctccga tcgtgtccat ccggccgaca tcgctgaggt
     4141 cgatcaggaa attgtcgccc ttgagccgct cgagccgggt cggcagcacc ccgagccggg
     4201 acaaggtgag gtcgccgtgg aagcgcagca cgcgccggcc gttctcgctg gtctcggtga
     4261 agcgggcgac ggtgctcatg ggcgggtcat gcttcaattc cctttggcgg gcaactggac
     4321 aaggcacgcc gcggatgctt cacgccgatt cgatgaaggc gcggctcgcc atatacgaca
     4381 tggaccggac gatcacccgc cgggccacct acacgccgtt cctgatccat gcggccctcc
     4441 gtctcgcgcc gtggcggctc gcgctgctgc cgctcgtcct gctggcgatg ggcgcctatg
     4501 tcctcaagct gatcgaccgc ggccggctga aggagatcaa ttacgggttg ctggtcggcc
     4561 gcgccgtcgc tctcgggcgg ctcgagccgg tgatcgagag cttcgccgac gcccagctcg
     4621 ccggcaacat catgccgggc gcgcgccggg ccatcgccgc ggacaaggcg gcggggcggc
     4681 ggctggtgat ggcgaccgcc tcctaccggc tctacgccgc cgcgatcgcg aagcggctcg
     4741 gcttcgacga cgtcgtcgcc accgagaccg gcctcgacac cgagggccgg gtggtcgcca
     4801 agatcgacgg caccaattgc tacggcccgg ccaagaagga catgatcctg gcctggctgc
     4861 agcgcgaagg gctggagcgc gaggccgtcc atatccgctt ctactccgac cacgtctccg
     4921 accaccacgt ccaccgctgg gccgacgagg cggtcgcggc caatgcccac gcccggctgg
     4981 tcaggctggc cagggcggaa ggatgggagg tggtggactg gtcggaccgg tgatccccaa
     5041 agtcctcccc gtgcgcgaag cgcgtgggga gggggaccgc gtgaaacgcg gtggagtggc
     5101 agcgacgaca gcgagatttg gccgccgaca ggatcaggtc caccgcgggt tcgatgtcgc
     5161 cgtaaaggtg ccgagcaggg atgcggcacg ggcggaagcc gcgccccctg agccaatcgt
     5221 ctcgtgccac atcatgagta gggctttcgc ccatcccgtg agcaacaccg tcgacctcga
     5281 tgccgagttt cgaggaaggg caatagaagt cgagcacata ggggcccaca ggatgctgtc
     5341 ggcgggactt caggccgccg gggcgggtcc gcaagacctg ccataagcac gcctcaggtc
     5401 cggtcaatcg tccggcgaag cgcccgggtc ttcgcaaccg tggctttggt cgtatttcgc
     5461 attgcctgat cgcagcaccc cccctggccg aggccgatgg gaagggggga caagcgaacc
     5521 ctgctgtggg gctatcgccg ctccgaaatc gcccccccac cgcacttcgc gcggtccccc
     5581 tccccatccg ctgcgcggac ggggaggttg ccgatcagcg ctttagcgct cgaaggtcga
     5641 cgtttccagg gagcgtgggc ccggaaattt ctattatcgc ctaatcctac accgccccca
     5701 gcgcctggtc gaagtcggcg atcaggtcgt cggggtcctc gacgccgatc gagacgcgca
     5761 ccagattgtc ggtgatgccc agcgcctgct tgcgctcgtc gggcacggag aggtgcgtca
     5821 tcgccgccgg cgcgctcgc
//
LOCUS       DASVQZ010000216         6515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_241301, whole genome shotgun sequence.
ACCESSION   DASVQZ010000216 DASVQZ010000000
VERSION     DASVQZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241301"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..574)
                     /locus_tag="VF547_05890"
     CDS             complement(197..574)
                     /locus_tag="VF547_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX8262387.1"
                     /translation="MPIDATRPYDDSNVFARILRGEIPARTVYEDDHALAFHDINPAA
                     PVHVLVIPKGRYVSWDDFSERASAEEIAGFVRAVGHVAREQGLVAAGYRLLANVGINS
                     GQEVPHLHVHLFGGRPLGPMLSR"
     gene            670..1152
                     /locus_tag="VF547_05895"
     CDS             670..1152
                     /locus_tag="VF547_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX8262388.1"
                     /translation="MQGETPDSLIPYDEIVQEALRAVVGRVLGEVETTGRLPGEHHFY
                     ITFKTGAAGVEIPKHLSERFPDEMTIVIQNRFWDLKVGEDGFEVGLSFNQAPARLVIP
                     FSAITGFVDPAVNFALQFQAQAEESGPQGHEAAENDPPAAEPVEDGSNVVSVDFSRKK
                     "
     gene            1265..2665
                     /gene="fumC"
                     /locus_tag="VF547_05900"
     CDS             1265..2665
                     /gene="fumC"
                     /locus_tag="VF547_05900"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX8262389.1"
                     /translation="MIAADTLRIETDSFGPIDVPGDAYWGAQTQRSIQNFPFGPREVM
                     PVEVVHALGFVKQAAARVNARSGALDAEIGEAIQQAAGEVATGVHDDQFPLVIWQTGS
                     GTQSNMNANEVIAGRANEILTGTRGGKSPVHPNDHVNKGQSSNDSFPTAMHVAAARAV
                     ESRLIPALQTMHRRLTAQAQAWDQIVKIGRTHLQDATPLTLGQEFSGYAAQVDAGIER
                     LRGVMPRLLQLGQGGTAVGTGLNAPKGFADSFAAEVAKLTKLRFTSAPNKFEALAAHD
                     TLVELSGVLNVIAVSLTKIANDIRLLGSGPRCGLGELKLPENEPGSSIMPGKVNPTQA
                     EMLTMVAAQVMGNHVAVTVGGLQGHLELNVFKPVIVANVLRSIDLLSTGVESFTERTL
                     DGLEPDERRIAELMNRSLMLVTALAPEIGYDNAAAIAKHAHKQGLTLKEAGLELGLVD
                     EETFDRVVRPEAMLGR"
     gene            complement(2662..4140)
                     /locus_tag="VF547_05905"
     CDS             complement(2662..4140)
                     /locus_tag="VF547_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX8262390.1"
                     /translation="MLDTALDAVVVMTREGMVAGWNGVAEQTFGWTGAEAVGRLMADL
                     IIPEQFREAHCRGLERYNATAEERVLNRRIEISAVDKQGREFPVELSITIAGHPDESL
                     FLGFLRDISQRREAEARLKRQARETELLFNVTRMAAETGSFDETLQICLEAICAMTGW
                     PIGHALIAGANGGELVSTGVWHEAGEADARAIRAVTDDMRFTAGVGLPGLILERGEPA
                     WISDAENNPDFARKGLGFAAAFGFPIKSDGRIVAVLEFFSRDAVEPDPDLMMTVRTVG
                     EQLGRVLERRRTEEHLRLLVNELNHRVKNTLAIVQSIAMQTLRGDGVGEKARRAFESR
                     LAALAAAHDLLTTENWESASLRQVIEKAGLGCGAPADRLRVSGPELRVQPRAAVSLAM
                     AVHELCTNAVKYGALSSDRGKVAVEWEVVGAGDSSRLRLRWRETGGPRVTPPETRGFG
                     TRMIERALAVELGGSARIEFLPEGVCCTVEAPLPAPGDPAAG"
     gene            complement(4243..4770)
                     /locus_tag="VF547_05910"
     CDS             complement(4243..4770)
                     /locus_tag="VF547_05910"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX8262391.1"
                     /translation="MKPILPVAALALALAAAPAEGAELFGGLYVHDVDTGLTKSGIEE
                     GVDFELGWRGARIGFLRALGGPRPQALVSVNSAGNTHFAAAGIGWKIGRRVYLRPAIG
                     IAVHTGPGRVVPGDDRIDFGSRILFAPELGAGARLTDRISVEASWVHLSHGQLFGPQN
                     PGLDTIGVRFNYRLR"
     gene            4842..5006
                     /locus_tag="VF547_05915"
     CDS             4842..5006
                     /locus_tag="VF547_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262392.1"
                     /translation="MSNEPVAPGDEAAPGTPGTGENICRHCNGTGQHDGVPCPVCDGT
                     GKVVEGLAGG"
     gene            complement(5009..5563)
                     /locus_tag="VF547_05920"
     CDS             complement(5009..5563)
                     /locus_tag="VF547_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HEX8262393.1"
                     /translation="MPRRYWYLAVALVGAAAAILLWMGRPAICTCGEVKLWVGTVNGP
                     DNSQHLADWYTPSHIIHGLLFYFLGWLFLRRNPPGDRLLAAVLIEAGWEILENSRWII
                     DRYREATMALGYSGDSVINSVADIGWMAVGFALARRLPVWAAILLAAAFELLTLAIIR
                     DNLTLNVIMLVTPSDSIRAWQAGA"
     gene            complement(5578..6384)
                     /locus_tag="VF547_05925"
     CDS             complement(5578..6384)
                     /locus_tag="VF547_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262394.1"
                     /translation="MRAAAMAALALLVAGCATRQPAPAQNRPLYNKAELAALPTAEVA
                     AKFLPPAEAARVEKHWIDEVVYLARDRLWDIHLRSRPRPIGADICERTDWTITFYPAK
                     RAAGDERPEMPAWAGDFHRRSDHALAPGCRDLPGLSFARDMGVGDDRLTDEQSIAILR
                     ALASAQAAAAGSSPLTFRLSCVNYSFEKDACAGDLRAILAGLPLHRAFSVERHPHPHN
                     CSGLARDEGDAVEIGDPEDRSVWDVRLRHLGTDKAEVSLIRQFSRTHVLC"
BASE COUNT         1053 a         2241 c         2255 g          966 t
ORIGIN      
        1 aggttccagg ggccgagtga ccgcttcagt tcgctggtct gggtttcccg ttggacctgg
       61 gcgaccgtct tgcggatcat gatgcgcccg aacaggcccc gtggagcggc actcgccata
      121 cttctacccc tttgtcgaca ctgtcgtcgg gcgcggaacc tagccagtta ttccgcgacc
      181 gcaaaggtct aaacgctcac cgcgacagca tcggtccaag cggcctgccg ccgaagagat
      241 ggacgtgcag atgcggcact tcctggccgg aattgatccc cacattggcc agcagccgat
      301 agcccgccgc gaccaggccc tgctcgcgcg cgacatggcc gacggcgcgg acgaagccgg
      361 ctatttcctc cgccgaagcc cgctccgaga aatcgtccca ggacacgtat cggcccttcg
      421 ggatgacgag gacgtggacc ggggccgccg gattgatgtc gtggaaggcc agcgcatggt
      481 cgtcctcgta gaccgtcctg gccggaatct cgccccgcag gatgcgggcg aagacgttgc
      541 tgtcgtcgta ggggcgggtg gcgtcgatcg gcatgaagtc gtgctccttg gcccgtctat
      601 ctccaacatg gataagacgg agaaggcaac cgggttgacc cacggcctcc cggcgttacc
      661 acggggagga tgcaaggtga gacgcccgac agcctgattc cctacgacga gatcgtccag
      721 gaggcgcttc gcgccgtggt cggccgggtc ctgggcgagg tcgagacgac gggacggctc
      781 cccggcgagc accatttcta catcaccttc aagaccggcg cggcgggcgt cgagatcccc
      841 aagcatcttt ccgaacgctt tcccgacgag atgacgatcg tcatccagaa ccgcttctgg
      901 gacctcaagg tcggcgagga cgggttcgag gtcggcctca gcttcaacca ggcgccggcg
      961 cggctggtca tccccttctc cgcgatcacc ggcttcgtcg acccggcggt caatttcgcg
     1021 ctccagttcc aggcccaggc ggaggagtca gggccgcagg gccacgaggc ggcggagaac
     1081 gatccgcccg ccgccgagcc ggtcgaggac ggctccaacg tcgtctcggt cgatttcagc
     1141 cggaagaaat aggcctccgc agcgctggga atccgttcgg gctgagcttg tcgaagccct
     1201 ctccttccct taaccgcgcg caagaaaggc ccttcgacaa gctcagggcg aacggagttc
     1261 ttgcatgatc gccgccgaca ccttgcgcat agaaaccgac agcttcggtc cgatcgacgt
     1321 ccccggcgac gcctattggg gcgcccagac gcagcgctcg atccagaact tccccttcgg
     1381 gccgcgcgaa gtcatgccgg tcgaggtcgt ccatgcgctc ggcttcgtca agcaggcggc
     1441 cgcgcgggtc aacgcccgca gcggcgcgct cgacgccgag atcggcgagg cgatccagca
     1501 ggcggcgggc gaggtggcga ccggagtcca tgacgaccag ttcccgctcg tcatctggca
     1561 gaccggctcg ggcacccagt ccaacatgaa cgccaacgag gtgatcgcgg gccgcgccaa
     1621 cgagatcctg accggcaccc gcggcggcaa gagcccggtc catcccaacg accatgtcaa
     1681 caagggccag tcctcgaacg acagcttccc gacggcgatg cacgtcgccg cggcacgggc
     1741 ggtggaatcg cggctgatcc cggcgctgca gacgatgcac cgccggctga cggcgcaggc
     1801 ccaggcctgg gaccagatcg tcaagatcgg acgaacgcat ctgcaggatg cgacgccgct
     1861 gacgctcggc caggaattct caggctatgc cgcgcaggtc gacgccggga tcgagcggct
     1921 caggggcgtc atgccgcggc tgctccagct cggccagggc ggcaccgcgg tcggcaccgg
     1981 cctcaacgcg cccaagggct ttgccgactc cttcgcggcg gaagtggcga agctcaccaa
     2041 gctgcgcttc acctcggcgc cgaacaagtt cgaggcgctc gccgcccacg acacgctggt
     2101 cgagctgtcg ggcgtgctca acgtcatcgc cgtgtcgctg accaagatcg ccaacgacat
     2161 ccgcctgctc ggctccgggc cgcgctgcgg cctcggcgag ctgaagctgc cggagaacga
     2221 gccgggcagc tcgatcatgc cgggcaaggt caacccgacc caggcggaga tgctgaccat
     2281 ggtcgccgcg caggtgatgg gcaaccatgt cgccgtcacc gtcggcggcc tccagggcca
     2341 tctcgagctg aacgtgttca agccggtgat cgtcgccaac gtgctgcgct cgatcgacct
     2401 gctgtccacc ggagtcgaga gcttcacgga gcgcaccctc gacggcctcg agcccgacga
     2461 gcggcgcatc gccgagctga tgaaccgctc gctgatgctg gtgacggcgc tggcgcccga
     2521 gatcggctac gacaacgccg ccgccatcgc caagcacgcc cacaagcagg ggctgacgct
     2581 gaaggaggcg gggctggaac tcggcctggt cgacgaggag acgttcgacc gggtggtccg
     2641 gccggaggcg atgctgggcc gctagccggc ggcgggatcg ccgggcgcgg gcagcggcgc
     2701 ctcgaccgtg cagcagaccc cctcgggcag gaactcgatg cgcgcggatc cgccaagctc
     2761 gaccgccagc gcccgctcga tcatcctcgt tccgaagccg cgggtctccg gcggcgtgac
     2821 ccgcggaccg ccggtctcgc gccagcgcaa gcgaagccgg ctcgaatccc ccgccccgac
     2881 cacctcccac tcgacggcga ccttgccccg gtcgctggag agggcgccgt atttgacggc
     2941 gttggtgcac agctcatgga ccgccatcgc cagcgacacc gcggccctgg gctggacgcg
     3001 cagctccggt ccggaaacgc gcagccggtc cgcaggcgca ccgcagccca gtccggcctt
     3061 ctcgatcacc tgccggagcg aggcggactc ccaattctcc gtggtgagaa ggtcgtgcgc
     3121 ggcggcgagg gcggcgaggc ggctctcgaa ggcgcgccgc gccttttcgc cgacgccgtc
     3181 gccgcgcagg gtctgcatgg cgatgctctg gacgatggcg agcgtgttct tgacccggtg
     3241 gttgagctcg ttgaccagca gccgcaaatg ctcctcggtg cggcgccgtt ccagcaccct
     3301 tccaagctgc tcgccgaccg tcctcacggt catcatcagg tccggatccg gctcgacggc
     3361 atcgcggctg aagaattcga ggaccgccac gatccttccg tcgctcttga tcgggaagcc
     3421 gaacgcggcg gcgaaaccca gccccttgcg ggcgaaatcc ggattgttct cggcgtccga
     3481 gatccaggcc ggctcgccgc gctccagaat gagaccggga aggcccacgc cggcggtgaa
     3541 acgcatgtcg tcggtcaccg cccggatggc ccgggcgtcg gcttcccccg cctcgtgcca
     3601 cacgccggtc gaaaccagct cgccgccatt ggcgccggcg atgagcgcgt ggccgatcgg
     3661 ccagccggtc atggcgcaga tcgcttccag gcagatttgc agcgtttcgt cgaaggagcc
     3721 ggtttcggcg gccatccggg tgacgttgaa cagcagctcg gtctcgcgcg cctggcgctt
     3781 gaggcgggcc tccgcttcgc ggcgctggct gatgtcgcgc aggaagccga ggaaaagcga
     3841 ttcgtcgggg tggccggcga tggtgatcga cagctcgacc ggaaactccc ggccctgctt
     3901 gtcgaccgcg ctgatttcga tgcgccggtt gagcacgcgc tcctcggcgg tcgcgttata
     3961 ccgctcgagg ccgcggcaat gcgcttcgcg gaattgctcg ggaatgatca gatccgccat
     4021 gagccggccg acggcttccg cgcccgtcca gccgaaggtc tgctcggcga ctccgttcca
     4081 gcccgcgacc atgccttccc gggtcatcac gacgacggcg tcgagcgcgg tatcgagcac
     4141 cggcccaagt gcctcaattg ccgcggtttt cgatttctgg acgtgcaccg tttcggatta
     4201 gggacgaaac ggtttcgtgt cgattgccga tttggccgcc ggctagcgga gccggtagtt
     4261 gaagcggacg ccgatcgtgt cgaggcccgg attttgaggc ccgaacagct ggccgtggct
     4321 gagatggacc cagctcgcct cgaccgagat ccggtcggtc agccgggcgc cggcccccag
     4381 ctccggcgcg aacaggatgc gcgagccgaa atcgatccgg tcgtcgccgg gcacgacgcg
     4441 gcccggcccg gtgtggacgg cgattccgat ggcggggcgc agatagaccc gccgcccgat
     4501 cttccagccg atcccggcag ccgcgaaatg ggtgttgccg gcgctgttga ccgaaaccag
     4561 ggcttgcggc ctcggcccgc cgagggcgcg aaggaagccg atcctggcgc cgcgccagcc
     4621 cagctcgaaa tccacacctt cctcgatccc gctcttggtg agaccggtgt cgacgtcgtg
     4681 gacatacagg ccgccgaaga gctccgcccc ctcggccggg gcggcggcga gggcgagggc
     4741 gagagccgct accgggagaa tcggcttcat ggccggcaaa cccgtggccg gccgctgggg
     4801 ttcccccggg aaccgggccg gggggcgggg gttgcacggc catgagcaac gaacctgtcg
     4861 cgccgggcga cgaagcggcc cccggcaccc ccggcaccgg cgagaatatc tgccggcact
     4921 gcaacggcac cggccagcat gacggggtcc cctgccccgt ctgcgacggc accggcaagg
     4981 tcgtcgaggg cctggccggc ggctgagatc atgcgccggc ctgccaggcg cggatcgaat
     5041 cgctcggggt gaccagcatg atgacgttga gcgtcagatt gtcgcggatg atcgccaggg
     5101 tcagaagctc gaacgcggcg gccagcagga tcgccgccca gaccggcagc cgccgcgcga
     5161 gcgcgaagcc cacggccatc cagccgatgt cggcgaccga gttgatcacg ctgtcgccgg
     5221 aatagccgag agccatggtc gcctcgcgat agcggtcgat gatccagcgg ctgttctcga
     5281 gtatctccca gcccgcctcg atcagcaccg cggcgagcag ccggtcgccc ggcgggttgc
     5341 ggcgcaggaa cagccagccg aggaagtaga agagcaggcc gtggatgatg tggctgggcg
     5401 tgtaccagtc ggcgagatgc tggctgttgt cgggcccgtt gaccgtcccg acccagagct
     5461 tcacctcgcc gcaggtgcag atcgccggcc ggcccatcca cagcaggatc gcggccgcgg
     5521 ctccgaccag cgcgacggcc agataccagt agcgccgcgg gatgaggggt gcgccgctca
     5581 gcacaggacg tgggtccggg agaattggcg gatcagggag acctcggcct tgtcggtgcc
     5641 gaggtggcgc agccgcacgt cccacaccga ccggtcctcc gggtcgccga tctcgacggc
     5701 gtcgccctcg tcgcgcgcaa ggccgctgca attgtgcggg tggggatggc gctcgacgct
     5761 gaaggcacgg tggagcggca agccggcgag aatcgcgcga aggtcgcccg cacaggcgtc
     5821 cttctcgaag ctgtagttga cgcagctcag acggaaggtc agcggcgagg agccggcggc
     5881 ggcggcctgg gccgaggcca gggcgcgcag gatcgcgatg ctctgctcgt cggtgaggcg
     5941 gtcatcgccg actcccatgt ccctggcgaa gctcagcccc ggaaggtcgc ggcatcccgg
     6001 cgcaagcgcg tggtcggaac ggcggtggaa gtcgcccgcc caagccggca tttcaggccg
     6061 ctcgtccccg gccgcgcgct tggcggggta gaaggtgatc gtccagtccg tgcgttcgca
     6121 gatatcggcg ccgatgggcc tcgggcgcga gcggagatga atgtcccaca gccgatcgcg
     6181 cgcgagatag acgacctcat cgatccaatg cttctcgacc ctcgccgctt ccgccggggg
     6241 gaggaatttc gccgccacct cggccgtcgg gagcgcggcg agctcggcct tgttgtagag
     6301 tgggcggttc tgtgccggcg ccggctgtcg cgtcgcgcag ccggcgacga ggagcgcgag
     6361 cgccgccatc gccgccgctc tcatttcgcc atctcctcga gcttcaggac ggtccgccag
     6421 ttgcgggcgg tcgagggcga gccgcaggcc ctgtcgatca gcgacggggt gagcttcgac
     6481 cggccgacgc cctcgggata gtcgagccac aggac
//
LOCUS       DASVQZ010000217         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_334663, whole genome shotgun sequence.
ACCESSION   DASVQZ010000217 DASVQZ010000000
VERSION     DASVQZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334663"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VF547_05930"
     CDS             complement(<1..853)
                     /locus_tag="VF547_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8262395.1"
                     /translation="MKLEFDIAVVGGGHAGCEAAAAAARMGARTALISFARDQIGAMS
                     CNPAIGGLGKGHLVREVDAFDGLIGRASDAAGLHYRMLNASKGAAVRGPRVQADRRLY
                     KSEIHRLLDGQPSLEIVEGEVVELLVAGRRVEGAVLVDGRQLRCGAVVLATGTFLGAK
                     LHFGMTSRAGGRIGERAATALASRLRALGLPLARLKTGTPPRLDGRTIDWAARERQES
                     DAEGWTMSPMSAGRPAPQLFCAITRTNPATHALIRRNLDRSPLFAGEISGVGPRYCPS
                     IEDKVHRF"
     gene            complement(885..2156)
                     /gene="mnmE"
                     /locus_tag="VF547_05935"
     CDS             complement(885..2156)
                     /gene="mnmE"
                     /locus_tag="VF547_05935"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX8262396.1"
                     /translation="MTDTIFALSSGSPPAALAVVRVSGPRADSALEALAGKRPEPRVA
                     TLANLEWKGEPLDQALVLRFPGPHSATGEDVAELHLHGGRAVVAAVLEALAQLDGLRA
                     AEPGEFTRRAFDNGRIDLAEAEGLADLLAAETQSQRRSALAAAGGALSRQVESWRQRL
                     LELAAALEAALDFSDEGDVGENWPEGWREGQLGLAGEVRAQLGRPPAERLRDGVRVVI
                     AGPPNSGKSSLLNALVQRDAAIASAVPGTTRDVIEAPTAIAGMPFVLIDTAGLRETSD
                     EVEAIGVARARSTLESADVVLWLGDPDDAPPAARTVAIRSKCDLGDGGSDGRLAVSVK
                     TGEGLAALVEAIVARAAELLPTAGDVPLNQRQRAALRQCLAHLDEIGEADMLLAAEAL
                     RLARGELDRITGRAGVEDMLDSLFGRFCIGK"
BASE COUNT          313 a          877 c          806 g          335 t
ORIGIN      
        1 cgaagcggtg gaccttgtcc tcgatcgacg ggcagtagcg cggtccaacg ccgctgatct
       61 ccccggcgaa gagcggcgag cggtcgagat tgcggcggat cagcgcgtgc gtggccgggt
      121 tggttcgggt gatcgcgcag aagagctggg gcgccggccg ccccgcgctc atgggcgaca
      181 tcgtccagcc ctcggcgtcg gactcttggc gttcgcgcgc cgcccaatcg atcgtccgac
      241 cgtcgagccg cggcggcgtg cccgtcttga gccgggcgag cggaagcccc agcgcgcgca
      301 gccgactcgc cagggcggtc gccgcgcgct ccccgatccg gccgcccgcg cggctggtca
      361 ttccgaaatg cagcttcgcg cccaggaagg tgccggtggc gaggaccacc gccccgcagc
      421 ggagctggcg gccgtcaacg agcaccgctc cctcgacccg tcggccggcg acgaggagct
      481 cgacgacctc gccttccacg atctccaggc tgggctggcc gtccagcagc cggtggatct
      541 cggacttgta gagtcggcgg tccgcctgga cccgcgggcc gcgcaccgcc gcccccttgc
      601 tggcgttgag catccggtag tggagtccgg cggcgtccga ggctcggccg atgagtccgt
      661 cgaacgcgtc gacctcgcgg acgagatggc ccttgccgag cccgccgatc gcgggattgc
      721 aggacatcgc cccgatctgg tcccgggcga agctgatcag cgcggtgcgc gcacccatcc
      781 gcgccgccgc ggcagcggct tcgcagcccg cgtggccccc gccgaccacg gcaatgtcga
      841 attcgagctt cacagccgcg acttactgtt ccacgtggaa catgtcactt gccgatgcag
      901 aaacggccga acaggctgtc gagcatgtcc tcgacgcccg ctcggcccgt gatccggtcg
      961 agttctcccc gtgcgagccg aagcgcttcg gcggcgagca gcatgtcggc ttcgccgatc
     1021 tcgtccagat gcgcgaggca ttgccgcaag gcggcacgct gccgctggtt gagggggacg
     1081 tcgcctgcag tcggcaaaag ctcggcggcc ctagcgacga ttgcctcgac cagggccgca
     1141 aggccttcgc cggtctttac ggaaaccgcg aggcgcccgt cgctgccccc gtcgcccaga
     1201 tcgcatttcg aacggatggc aacggtgcgc gcggccgggg gcgcatcgtc cgggtcgccc
     1261 agccacagca cgacatccgc cgactcgagc gtcgagcgcg cccgcgccac gccgatcgcc
     1321 tcgacctcgt cactcgtctc ccgaagcccc gcggtgtcga tcaggacgaa cggcattccc
     1381 gcgatcgccg tcggcgcctc gatgacgtcg cgcgtggttc ccgggacggc cgaggcgatg
     1441 gccgcgtcgc gttgcactaa ggcattgaga aggctggact ttccagagtt tggcgggccg
     1501 gcgatgacga cccggacccc gtcccgcaag cgttcggccg gcggccggcc aagctgggcc
     1561 cggacctcgc cggccaagcc gagttgcccc tctcgccagc cctcgggcca attttccccc
     1621 acgtcgcctt cgtcggagaa atcgagcgcc gcctccagcg ccgccgccag ctcgagcagc
     1681 ctttgacgcc atgactccac ctgccggctc agcgctccgc cggccgcggc gagcgccgac
     1741 cggcgctgcg actgcgtctc cgcggcgagc aggtcggcga ggccctccgc ctcggccagg
     1801 tcgatccgtc cgttgtcgaa ggcgcggcgg gtgaactcgc cgggttcggc ggccctcagg
     1861 ccgtcgagct gcgcgagcgc ctcgagcacc gccgccacca ccgcccggcc gccgtggaga
     1921 tggagttccg ccacatcctc gccggtggcg ctgtggggcc ccggaaagcg caggacgagg
     1981 gcctggtcga ggggctcgcc cttccactcc aggttcgcca gggtcgccac ccgcggctcg
     2041 ggccgcttgc cggcgagcgc ttcgagagcg gagtccgcgc gcgggccgct gacccggacg
     2101 acggccagag cggcgggggg gcttcccgag gacaaggcga agatggtgtc ggtcacgtcc
     2161 cggctggcct ccgctcaacc cttcttcccg cccatcccct ccagcatcga gcgccacatc
     2221 gccaggcccg cctcgttcat cgggccccag gccttcatca tctcggcggc cgattcggcg
     2281 gtgaggccct cgccggcggc cttggccatg cgctggacat aggcctcgtg g
//
LOCUS       DASVQZ010000218         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_816378, whole genome shotgun sequence.
ACCESSION   DASVQZ010000218 DASVQZ010000000
VERSION     DASVQZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816378"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VF547_05940"
     CDS             complement(<1..548)
                     /locus_tag="VF547_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8262397.1"
                     /translation="MRRTAALIVGGGPAGSAAAIGLARSGIETALVERSEGPRDMVCG
                     GFLGWDSLAALERLGVDPGSLGARPIRTLRLISSSRQVEVPLPRLAAGLSRRSLDEAL
                     LAEAGRAGASIRRGRTVRAADLAGRSLRLDGDEAVAGDALVLATGKHELRGAARPIDI
                     SSRPLGLRTSIEPGRRLGASLDG"
     gene            complement(527..1216)
                     /locus_tag="VF547_05945"
     CDS             complement(527..1216)
                     /locus_tag="VF547_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8262398.1"
                     /translation="MRSLGQRSRDEEQMDDPSLDPTTYDEVLKGLGRVNGWTFAARPT
                     LAFLARATRGLDRFTLLDVGSGEGGMLRVIARWARKRGLDARLTGVDLNPKSESVARA
                     STPPDLPIAYRTGDYRDLGRFDFVVSSLVAHHMTDDQLSDFLRWMEANAALGWFVNDL
                     HRHRVAHSGYPLLAALLRVHPIVRQDGRLSIARAFRPSEWRSILDESGIAPGVRVRRY
                     FPFRLCVERLR"
     gene            complement(1213..>2122)
                     /locus_tag="VF547_05950"
     CDS             complement(1213..>2122)
                     /locus_tag="VF547_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX8262399.1"
                     /translation="VLPPAPYGASPVDPGGFYHGALPTTGARMEIYAQAAPKLALEAI
                     GKLNGAAAPEGITHLVVASCTGFVAPGIDQIIAAELGLGGHVERTLVGFMGCYAAVSS
                     LRLAHHIVRSEPEARVLVVTVELSSLHLQYEEDVERLLAALQFGDGASASLVTAEPSG
                     FALSRPFAATLPDSNALIRWRVGDHGFVMDLSGEVPGRIGEALRDRGVREAMLGNESP
                     EAFDSWAVHAGGRSVLDSVEHGLGLAPEALAPSRDILSRFGNMSSSTLPFVLQRLLAE
                     PERRRGIALAFGPGLAAEGFHFERAA"
BASE COUNT          341 a          759 c          765 g          257 t
ORIGIN      
        1 ccgtcgagag aggcgccgag ccgccgcccg ggctcgatcg acgtcctcag gcccagcggc
       61 cggctcgaga tgtcgatggg gcgggcggcg ccccgaagct catgcttgcc ggtggcgagc
      121 accagggcat cgcctgccac cgcctcgtcg ccgtccaggc gcaaggaccg ccccgccagg
      181 tcggccgccc gaacggttcg tcccctccgg atcgaggctc cggcccggcc ggcttcggcc
      241 agcagcgcct cgtccaggct acggcgggag aggccggcgg ccagcctggg cagcggcacc
      301 tcgacctggc ggctcgagga gatgagccga agcgtcctga tcggccgggc tccgagcgat
      361 ccgggatcca ccccgagccg ctccagggcc gcaagcgagt cccagccgag aaagcctccg
      421 cagaccatgt cgcgcggccc ctcgcttcgc tcgaccaaag ccgtctcgat cccggagcgg
      481 gcaaggccga tggcggcggc cgaccccgcc ggcccgcccc cgacgatcag cgcagccgtt
      541 cgacgcatag ccggaacggg aaatagcggc ggacccggac cccgggggcg atcccggatt
      601 cgtccaggat cgaccgccat tcggaaggtc ggaaggcccg ggcgatcgag agccggccgt
      661 cctgccggac gatcgggtgg acccggagca gggccgcgag cagcgggtag ccggaatgag
      721 cgacccggtg ccggtgaagg tcgttgacga accagccgag cgccgcattc gcctccatcc
      781 agcgcaggaa gtcgctcagc tggtcgtcgg tcatgtggtg ggcgacgagg ctgctgacga
      841 cgaaatcgaa ccggccaaga tcgcgatagt cgcccgtgcg gtaggcgatc ggaaggtccg
      901 gcggagtcga cgcccgggcc acgctctcgc tcttggggtt gagatcgact ccggtgaggc
      961 gggcgtcgag gcctcgcttg cgcgcccatc gggcgatcac ccgaagcatc ccgccttcgc
     1021 cggagccgac gtcgagcagg gtaaaccggt cgagcccgcg ggtcgcccgg gcgaggaagg
     1081 ccagggtggg gcgggccgcg aaggtccagc cgttgacccg ccccaggccc ttcagaacct
     1141 cgtcgtaggt ggtcgggtcg agcgacgggt cgtccatctg ctcttcgtcg cggctgcgct
     1201 gccctaacga cctcacgccg cccgctcgaa gtggaagccc tccgccgcca ggccggggcc
     1261 gaaggccagg gcgatcccgc gccgccgctc cggctcggcg agcagccgct ggagcacgaa
     1321 cggcagggtc gacgaggaca tgttgccgaa gcgggacaat atgtcgcgcg agggcgcaag
     1381 cgcctcgggg gcaagcccca gcccgtgctc gaccgaatcg agcaccgagc ggccgccggc
     1441 atggaccgcc cagctgtcga acgcctcggg cgattcgttg ccgagcatgg cctcgcgaac
     1501 gccgcggtcg cgaagcgcct cgccgatccg gcccggaacc tcgccggaca ggtccatgac
     1561 gaagccatgg tcgccgaccc gccagcggat cagggcgttc gaatctggaa gggtggcggc
     1621 gaagggccgc gacagcgcga agccgctcgg ctccgccgtc accagcgagg cgctcgctcc
     1681 gtcgccgaac tgcagggccg cgagcagccg ctcgacatcc tcctcatatt ggagatgaag
     1741 gctggacagt tcgaccgtca ccaccagcac ccgagcctcg ggctcggacc ggacgatatg
     1801 gtgggcgagg cgaagcgagg acaccgccgc gtagcagccc atgaacccca ccaaagtccg
     1861 ctcgacatgg ccgccgaggc cgagctccgc cgcgatgatc tggtctatgc ccggagcgac
     1921 gaagccggtg cagctggcga cgaccaggtg ggtgattcct tcgggcgccg cggccccgtt
     1981 cagcttcccg atcgcctcga gcgccagctt cggagccgcc tgggcgtaga tctccatcct
     2041 ggccccggtg gtcggaaggg cgccgtggta gaagccgccc ggatcgaccg gggaagcgcc
     2101 gtagggcgcc ggcggaagga cc
//
LOCUS       DASVQZ010000219         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_46219, whole genome shotgun sequence.
ACCESSION   DASVQZ010000219 DASVQZ010000000
VERSION     DASVQZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46219"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF547_05955"
     CDS             <1..193
                     /locus_tag="VF547_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX8262400.1"
                     /translation="QAVAIGGGLGLRLAGHLPRSGFRDRFIAKGRFERRMDEMPVKII
                     THPQPGLFGAAAAFAAEHG"
     gene            335..697
                     /locus_tag="VF547_05960"
     CDS             335..697
                     /locus_tag="VF547_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262401.1"
                     /translation="MRLLPFLLLAAGCATGSAREADLSGELAGRSAGKAEECVPASTG
                     SNLTARDPRTLVYREGGTVWVNRLAADCPGLRESATLIVELHGSRYCRGDRFRTVEPG
                     SSIPGPSCLLGAFTPYRR"
     gene            757..830
                     /locus_tag="VF547_05965"
     tRNA            757..830
                     /locus_tag="VF547_05965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Gly,seq:ccc)
     gene            complement(845..1603)
                     /locus_tag="VF547_05970"
     CDS             complement(845..1603)
                     /locus_tag="VF547_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX8262402.1"
                     /translation="MTTERIWTAGLLVIGDEILSGRTQDRNIAQIGGWLNLQGIRLNE
                     VRVVPDRVERIGAAVNALRIENDYLFTTGGIGPTHDDVTVDAIAAALGVPVVVHPRAR
                     AILEAYYATRGGLTDARLRMARVPQGAELIENRISGAPGIRLGNIFILAGVPHIATLM
                     LEALSGTIEGGRPLLSRTVGCWVAESEVADLLAEVERAHPGCQIGSYPFFREGRVGAN
                     FVVRSPDAPALAACEAALVARLEQAGRDVVREGI"
     gene            1677..>1914
                     /locus_tag="VF547_05975"
     CDS             1677..>1914
                     /locus_tag="VF547_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX8262403.1"
                     /translation="MTEYQAISGTELIGRSGAIKLHGPEAFEGMRRAGRAAAEVLDML
                     VPHVVPGITTQAIDDIVHREMIARGGVPATLGYRG"
BASE COUNT          268 a          698 c          669 g          279 t
ORIGIN      
        1 gcaggcggtg gcgatcggcg gcggcctcgg gctgaggctt gcgggccacc tgccgcgatc
       61 cggcttccgc gaccgcttca tcgccaaggg ccgcttcgag cggcggatgg acgagatgcc
      121 ggtcaagatc atcacccacc cgcagccggg cctgttcggc gccgccgccg ccttcgcggc
      181 cgagcatggc taggggggtg gtcgccggtc ggtccggccg caactccgct ccccgacgaa
      241 ggtcggggcc cggcgcgcct gcgcaaaggc tggaccccgg ccttcgccgg ggggcatagg
      301 agcgcaacaa gcgccgcttc aagggaggcc ccccatgcgc ctgcttccgt tcctgctcct
      361 cgccgccggc tgcgccaccg ggtccgcgcg cgaggccgat ctgtcgggcg agctcgccgg
      421 ccgctcggcc gggaaggccg aggaatgcgt cccggcctcg accggctcga acctgaccgc
      481 ccgcgatccg cggaccttgg tctatcggga gggcggcacg gtctgggtca accggctcgc
      541 cgccgactgc cccggccttc gcgagtccgc caccctgatc gtcgagctcc acggctcgcg
      601 ctactgccgc ggcgatcgct tccggaccgt cgagccggga agctccatcc ccgggccgag
      661 ctgcctgctc ggcgccttca cgccctaccg gcgctagcgg ctcgctccag gagtctcgac
      721 aagcgccgcc gccttaccta gatatcggcg gtccgagcgg gcgtagctca atggcagagc
      781 agaagcttcc caagcttacg acgagggttc gattcccttc gcccgctcca ccgaccgcct
      841 cggctcagat cccttccctc acgacgtcgc gccccgcttg ctcgaggcgg gcgaccagcg
      901 ccgcctcgca ggcggcgagg gccggggcgt cgggagagcg gacgacgaaa ttggcgccga
      961 cgcgcccttc gcggaagaag gggtaggagc cgatctggca gccggggtgg gcccgctcga
     1021 cctccgccag caggtcggcg acctcgcttt cggccaccca gcagccgacc gtgcgcgaaa
     1081 gcaggggacg gccgccctcg atcgtccccg acagcgcttc cagcatcagg gtcgcgatgt
     1141 gcggcactcc ggcgaggatg aagatgttgc cgagccttat gccgggcgcg ccggagatgc
     1201 ggttctcgat cagctcggcc ccctgcggca cccgagccat ccgcagccgg gcgtcggtga
     1261 ggccgccgcg ggtcgcgtaa taggcctcga ggatggcccg cgcgcgggga tggaccacga
     1321 ccggcacgcc gagcgcggcg gcgatcgcgt cgacggtgac gtcgtcgtgg gtcgggccga
     1381 ttccgccggt ggtgaacaga tagtcgttct cgatcctcag cgcgttcacc gcggccccga
     1441 tccgctcgac ccggtcgggc accacccgga cctcgttgag ccggatgccc tgcaggttca
     1501 gccagccgcc gatctgggcg atgttcctgt cctgggtgcg cccggagagg atctcgtcgc
     1561 cgatgacgag caggccggcc gtccagatgc gttcggtggt caagcatatg ctccgctaaa
     1621 gccgctcggg ctgaacggcg tttgtgccgg gccgttccag agcctatatc catcccatga
     1681 ccgaatatca ggcgatcagc ggcaccgaat tgatcggccg cagcggggcg atcaagctcc
     1741 atgggccgga agccttcgaa ggcatgcgcc gggccgggcg ggcggcggcc gaggtgctcg
     1801 acatgctcgt tccccacgtc gtccccggaa tcacgaccca ggcgatcgac gacatcgtcc
     1861 atcgcgagat gatcgcccgg ggcggggtcc ccgcgacgct cggctatcgc ggct
//
LOCUS       DASVQZ010000220         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_527304, whole genome shotgun sequence.
ACCESSION   DASVQZ010000220 DASVQZ010000000
VERSION     DASVQZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527304"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VF547_05980"
     CDS             complement(<1..273)
                     /locus_tag="VF547_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX8262404.1"
                     /translation="MTPLQQSLIAFTLAAALLTVTPGLDTALVLRTAAAEGARRALAA
                     ALGICLGCLAWGVLVAVGLGVLLQASELAYAVLKWIGAAYLLYLGVR"
     gene            312..788
                     /locus_tag="VF547_05985"
     CDS             312..788
                     /locus_tag="VF547_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8262405.1"
                     /translation="MDDTDLIPLDRGLLRLMRIRGAAAGLALIAAAAVGEIALRDAAP
                     VPPGLLLLPALLILGYLAWWSPQRRYRAWGYAMDGEELRLRRGVWTRVHTLVPLDNVQ
                     HIDLSQGPLERAVGVSSLVLHTAGTLHSQIVVPGLARDTAERMRDEIRARIREEGA"
     gene            785..2320
                     /locus_tag="VF547_05990"
     CDS             785..2320
                     /locus_tag="VF547_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8262406.1"
                     /translation="MSDGAGAERRLHPGTIALRFLKEAPSTLLALPAGLAFASRSGLS
                     GALALAALLGGLLLLAKWLAWRRFRYAVGPAEIVIESGILHRNRRSIPFDRVQDVDIE
                     RTFLARLFGLAKVRIETGAGGKDEGLLDSVSLAEAHRLRETVRSWRSDAVPAAGETAG
                     ETPAAQALFVMDVPRVLLFGVFNFSLVYIAGLFALLQTFDRFLPFDIYDPARWIGLVD
                     TYLPHRWTFGAIVAVLLVAVLLGSAAGVLRTLARDYGFRLSLEGDRFRREHGLFTRSE
                     AVIARRRVQLAQVSGGPLRGLFGWAGLSFQTLGAGSDRSGRQNAAPFARRAEIERVLA
                     EVAPLRLPPPPELARVSRRHILRSASSRLGPALIAILALAYWQKAALMLLALLPLLAV
                     AAALDRRFHRYALADDLLFVARGVWRQRLWLVPVANVQSLALSSGPIQRRLGLATLAI
                     DTAGASMLDAPRIVDLRADDARTLAAEIGRLRRSVLQDRRRRITPRAGNPAPRDSARP
                     CRS"
     gene            complement(2262..3182)
                     /gene="cysK"
                     /locus_tag="VF547_05995"
     CDS             complement(2262..3182)
                     /gene="cysK"
                     /locus_tag="VF547_05995"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX8262407.1"
                     /translation="MIAANILETIGRTPHIRINRLFGQSEVWVKSERANPGASIKDRI
                     ALAMIEDAERSGALEPGGTIIEPTSGNTGIGLAMVAAVKGYKLVLVMPDSMSIERRRL
                     MLAYGATFDLTPREKGMKGAIARAEELVAATPGSWMPQQFENPANLEVHVRTTAQEIL
                     ADFRESPIDILITGVGTGGHITACAKVLKGEWPGLQVFAVEPTLSPVLSGGQPGPHPI
                     QGIGAGFVPGIMDVSLLDGIIQVDPADAKEMARRAAREEGILVGISSGASLAAVRQKL
                     AEVGPGKRVLTFNYDTGERYLSVPDFLPEE"
     gene            3349..4791
                     /locus_tag="VF547_06000"
     CDS             3349..4791
                     /locus_tag="VF547_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX8262408.1"
                     /translation="MDSKISRRNLLKAGAAFTVVGGAQVFTSVPALADFAAGPTVRRN
                     ASTMAANDPILRGYRRAIQRMRALPNNNPCSWFYQAAIHGTTDPSNLPSWNTCHTDPS
                     FFWAWHRMYLYFFERIVRKYSDQYDWAIPYWDWTNPAERALPAPFRDSSSPLYDASRN
                     PGVNAGNPLSSSLGTSVNNAMTLLDYFNTQSSVSGPHGTVHVTVGGNMQSTLSAAQDP
                     IFWAHHAEVDRIWNLWLAQGGGRSSPIGNATWRNTTYTFFDECCQPVQMKGCDVLRAA
                     LQLSYTYQGEPPQVNQYCPIIWNPQIFDVAVLAQSKTAIQLGGRPIRVPLLARDARAA
                     AGRLREAARTGERNIALQIRNVEAESQPGISWEVHVGRRGFTPDARSLVGIFALFGAG
                     LRDRKEHYHPAEFTFPIAKAIRGLDPATLEVTFVPVSGMEGEAAVAATALQPRTPVRI
                     GEVAILVDAQMPQAPRDEQERLRKQEQSER"
     gene            4837..>5258
                     /locus_tag="VF547_06005"
     CDS             4837..>5258
                     /locus_tag="VF547_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262409.1"
                     /translation="MALIHDVPRAFGEAECDSLVALAERAGLAPATVWSAAGDSVQAA
                     IRSAETSYHPRTPETAWIYERLDPLFAEAGEAFGLAVGPVTEPLQIVRYRVGGHFQTW
                     HSDAGFDALGRRLVSVSVELSAPGDHEGGDLEIIPDTVG"
BASE COUNT          762 a         1888 c         1778 g          830 t
ORIGIN      
        1 acgaacgccg aggtagagca gataggcggc gccgatccac ttgagcaccg cataggccag
       61 ttccgacgcc tgcagcagca cgccgaggcc gaccgcgacc aggacgcccc aggcgaggca
      121 gccgaggcag attccgagcg ccgccgccaa ggcccgccga gcgccctcgg cggccgcggt
      181 gcgcagcacc agcgcggtgt cgaggccggg agtcacggtg agcagcgcgg ccgcgagggt
      241 gaaggcgatc agggactgct ggagcggtgt catggtggac gcatagcaca ggcgcgcctt
      301 atggttgagc catggacgac accgatctga taccgctcga tcgcggactg ctgcggctga
      361 tgcggatccg gggcgcggcg gccggactgg ccctgatcgc ggcggcggcg gtcggcgaga
      421 tcgccctgcg cgacgccgcg ccggtgccgc ccggcctgct gctgctgccg gcgctgctca
      481 tcctcggcta tctggcgtgg tggtcgcccc agcgccgcta tcgcgcctgg ggctacgcca
      541 tggacggcga ggagcttcgg ctcaggcgcg gggtgtggac ccgggtccat acattggtgc
      601 cgctcgacaa cgtccagcat atcgacctga gccaggggcc gctcgagcgc gcggtcgggg
      661 tcagcagcct cgtcctccac acggcgggaa ccctgcacag ccagatcgtc gtgcccggcc
      721 tcgcccgcga caccgccgag cgcatgcgcg acgagatccg cgcccgcatc cgggaagagg
      781 gcgcgtgagc gacggagccg gcgccgaacg ccgccttcac cccggaacga tcgccttgcg
      841 cttcctcaag gaggcgccgt cgactctgct tgcgctgccg gcgggcctcg ccttcgcctc
      901 ccgatcgggg ctgtcgggcg cgctggcgct cgccgccctg ctgggcgggc ttctgctgct
      961 ggcgaaatgg cttgcctggc ggcgcttccg ctatgccgtc ggaccggcgg aaatcgtcat
     1021 cgaaagcggc atcctccacc gcaaccgccg ctcgatcccg ttcgaccgcg tccaggacgt
     1081 cgacatcgag cggaccttcc tcgcgcgcct cttcgggctc gccaaggtgc gcatcgagac
     1141 cggcgccggc ggcaaggacg aagggctgct cgacagcgtc tccctcgccg aggcccaccg
     1201 gctgcgcgaa acggtgcgca gctggcggag cgacgccgtc ccggccgcgg gcgaaacggc
     1261 gggcgagacg ccggcggcgc aggcgctttt cgtcatggac gtgccgagag tgctgctgtt
     1321 cggcgtcttc aacttctcgc tggtctacat cgccggcctg ttcgcgctcc tccagacctt
     1381 cgaccgcttc ctgcctttcg acatatacga tcccgcgcgc tggatcgggc tggtcgacac
     1441 ctatcttccc catcgctgga cgttcggggc gatcgtggcc gtgctgctcg tcgccgtgct
     1501 tctcggatcg gcggcgggcg tgcttcggac gctcgcgcgc gactacggct tccgcctgtc
     1561 gctggaaggc gaccgcttcc gccgggagca cggcctcttc acccgaagcg aggcggtgat
     1621 cgctcggcgg cgcgtgcagc ttgcccaggt gagcggcggg ccgctgcgcg ggctgttcgg
     1681 ctgggccggc ctctcgttcc agaccctggg cgccggcagc gaccgcagcg gccgccagaa
     1741 cgccgcgccc ttcgccaggc gcgcggagat cgagcgggtg ctcgccgagg tggcgccctt
     1801 gcggctgccg ccgccgccgg agctggcgag ggtctcgcgg cgccacatcc tgcgcagcgc
     1861 ctcatccagg ctcgggccgg cgctgatcgc cattctcgcc ctcgcctatt ggcagaaggc
     1921 ggcgctgatg ctgctggccc tgctgccgct gctcgccgtc gccgccgcgc tcgaccggcg
     1981 cttccatcgc tatgcgctgg cggacgacct tctgttcgtc gcccgcggcg tctggcggca
     2041 gcggctctgg ctggtgccgg tcgccaacgt ccagtcgctg gccctcagca gcggcccgat
     2101 ccagcggcgc ctcggcctcg ccaccctggc gatcgacacc gccggcgcct cgatgctcga
     2161 cgcgccgcgg atcgtcgacc ttcgcgccga cgacgccagg acgctcgcgg cggaaatcgg
     2221 ccgcctccgc cgatccgtcc tccaggatcg ccggcggagg atcactcctc gggcaggaaa
     2281 tccggcaccg agagatagcg ctcgcccgtg tcgtagttga aggtgaggac tcgcttcccg
     2341 ggtcccacct cggccagctt ctggcgcacc gcggcgaggc tggcgccgga gctgattccg
     2401 acgagaatgc cttcctcccg ggcggcccgg cgcgccatct ccttggcgtc ggccggatcg
     2461 acctggatga ttccgtcgag aaggctcacg tccatgatgc cggggacgaa gccggcgccg
     2521 atgccctgga tcgggtgcgg ccccggctgg ccgccggaaa ggaccggcga cagggtcggc
     2581 tcgaccgcga acacctgaag gccgggccat tcgcccttca gcaccttcgc gcaggcggtg
     2641 atgtggccgc cggtgccgac tccagtgatc aatatgtcga tcggcgactc gcggaagtcg
     2701 gcaaggatct cctgggccgt ggtccggaca tggacctcga gattggcggg gttctcgaac
     2761 tgctggggca tccagctgcc gggcgtcgcc gcgaccagct cctcggcccg tgcgatcgcg
     2821 cccttcatcc ccttctcgcg cggcgtgagg tcgaaggtcg cgccgtaggc cagcatcagc
     2881 cgccgccgct cgatgctcat gctgtcgggc atcaccagga cgagcttgta gcccttcacc
     2941 gcggcgacca tggcgaggcc gatgccggta ttgcccgagg tcggctcgat gatcgtcccg
     3001 cccggctcga gcgcgcccga gcgctccgca tcctcgatca tcgccagcgc gatccggtcc
     3061 ttgatcgacg cgcccggatt ggcccgctcg gacttcaccc acacctcgga ttggccgaaa
     3121 aggcggttga tgcggatgtg gggcgtccgc ccgatcgtct cgaggatatt cgcggctatc
     3181 attcgactct actccattcg tctgtgggcg tgttcgcgtc gggcttgagg gttcgcaaca
     3241 cataggaccg cggggtccga attcctattg ccctgcgctc cccgaacgcg atatgcggat
     3301 attgttgatt acccgaaaca tgagggggct catgaaggag ggatgatcat ggattccaaa
     3361 atcagccgtc gcaatctgct caaggccggt gccgccttca cggtcgtcgg tggcgctcag
     3421 gtcttcacca gcgttccggc gctggcggac ttcgccgccg ggcccacggt tcggcgcaac
     3481 gcgtccacca tggccgccaa cgatcccatc ctgcgcggct atcgccgggc catccagagg
     3541 atgcgggcgc ttccgaacaa caatccgtgc agctggttct accaggcggc gatccacgga
     3601 accaccgacc cctcgaacct cccgtcgtgg aacacctgcc acaccgaccc gagcttcttc
     3661 tgggcctggc accgcatgta tctctatttc ttcgagcgga tcgtcaggaa atattccgac
     3721 caatatgact gggccattcc ctattgggac tggaccaatc ccgccgagcg cgcgctgccg
     3781 gccccgttcc gcgactcctc gagcccgctc tacgatgcca gccgcaaccc cggcgtcaac
     3841 gccggcaacc ccttgtcgtc ctccttgggc acgtcggtca acaacgcgat gacgctgctc
     3901 gactatttca acacccaaag ttccgtcagc ggcccccacg gcaccgtcca cgtcaccgtc
     3961 ggcgggaaca tgcagtcgac cttgagcgcg gcgcaggatc cgatcttctg ggcccaccat
     4021 gcggaggtcg accggatctg gaacctgtgg ctcgcgcagg gcggcggaag gagcagcccg
     4081 atcggcaacg cgacctggcg gaacacgacc tacaccttct tcgacgaatg ctgccagccg
     4141 gtccagatga aaggctgcga cgtgctgcgc gccgccctcc agctcagcta cacctatcag
     4201 ggcgagcctc cgcaagtgaa ccaatattgc ccgatcatct ggaaccccca gatcttcgac
     4261 gtcgcggtcc tggcccagtc gaagacggcg atccagctgg gcggacgccc gatcagggtc
     4321 ccgctgctgg cgcgcgacgc ccgagcggcg gccgggcggc ttcgcgaagc ggcccggacg
     4381 ggcgagcgca acatcgcgct ccagatccgc aacgtcgagg cggagtccca gcccggcatc
     4441 agctgggagg tccatgtcgg ccgccggggt ttcacgcccg acgctcgaag cctggtcggc
     4501 atcttcgccc tgttcggggc gggcctcagg gaccgcaagg agcattatca tcccgccgag
     4561 ttcaccttcc cgatcgccaa ggcgatacgc ggactcgatc cggcgaccct cgaggtgacg
     4621 ttcgtgccgg tctccgggat ggaaggcgag gcggccgtgg ccgcgaccgc gctccagccg
     4681 cgcacgccgg tcaggatcgg cgaggtcgcc atcctggtcg acgcgcagat gccgcaggcg
     4741 ccgcgcgacg agcaggaaag gctgcgcaag caggagcaat ccgaacgata ggccgcggct
     4801 atcgcttccc gtgaagggag gcgctagcgt ggcgcgatgg cgctcatcca cgatgtcccg
     4861 cgcgcgttcg gcgaagcgga gtgcgactcg ctcgtcgccc tcgccgagcg cgccgggctg
     4921 gcgccggcga cggtgtggag cgccgccggc gactcggtcc aggcggcgat ccgctcggcc
     4981 gagacgagct accatccgcg gacgcccgaa acggcctgga tctacgagcg gctcgacccg
     5041 ctcttcgccg aggccggcga ggcgttcggc ctggcggtcg ggccggtgac cgagccgctg
     5101 cagatcgttc gctaccgcgt cggcggccat ttccagacct ggcacagcga cgccggcttc
     5161 gacgctctcg gtcgccgcct ggtctcggtc tcggtcgagc tctccgcgcc gggcgaccat
     5221 gaaggcggcg atttggagat catcccggac acggttgg
//
LOCUS       DASVQZ010000221         6367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_914886, whole genome shotgun sequence.
ACCESSION   DASVQZ010000221 DASVQZ010000000
VERSION     DASVQZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914886"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VF547_06010"
     CDS             <1..338
                     /locus_tag="VF547_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HEX8262410.1"
                     /translation="QVLTFVDLGPRLITVTHHDAVAGPYHRNGRDIIDVMRAFRGSAD
                     AARATIERRRIDYVLICPGLSESTIYASQAKGGFYTQLASGKAPAWLEPVPLPEKSPY
                     RMWRVVRGR"
     gene            complement(450..1073)
                     /locus_tag="VF547_06015"
     CDS             complement(450..1073)
                     /locus_tag="VF547_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX8262411.1"
                     /translation="MIALFLSAFATFFVVIDPPGCAPIFAGLTREAAAAERRSMAVRA
                     SLVAAGILLFFALLGEDLLRTLGISLAAFRIAGGIMLFLIALEMVFERRTRRRETRAQ
                     EVSADPGHDDVSVFPMAIPMIAGPGSIASIMLLMARSDGLDESLVVLAALAAVLLLTL
                     ASLLAAGWLMRLLGHRTEAMLTRLLGVILAALAAQFVIDGISVSFEV"
     gene            complement(1070..1960)
                     /gene="folD"
                     /locus_tag="VF547_06020"
     CDS             complement(1070..1960)
                     /gene="folD"
                     /locus_tag="VF547_06020"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX8262412.1"
                     /translation="MSAERIDGRAFAARLRERVAAAAPAFAQAVGRAPGLAVVLVGED
                     PASQVYVRSKGRATTEAGMASFEHRLPDSVSQEELLALVESLNEDESVDGILVQLPLP
                     AGIDDKAIIEAVDPAKDVDGFHPVNAGRLASGEGGLVPCTPLGCLMLLKDRLGDLAGL
                     EAVVIGRSNIVGKPMAQLLLAENCTVTIAHSRTRGLPGVVRRADIVVAAVGRAEMVRG
                     DWLRPGATVIDVGINRVAPEGEGKPRLVGDVHFDSASDVAAAITPVPGGVGPMTIAVL
                     LRNTLVAAHARAGLPPPEGL"
     gene            complement(1957..2259)
                     /locus_tag="VF547_06025"
     CDS             complement(1957..2259)
                     /locus_tag="VF547_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX8262413.1"
                     /translation="MLLALIDVVIILLKVVWWIVVIQMILSLLVAFNVINTYNDFVRG
                     LLRGLDRITEPVYRPIRRVLPDFGGLDFSPMVVLLVLWILNQILYDVAFSVAASYR"
     gene            2524..3531
                     /locus_tag="VF547_06030"
     CDS             2524..3531
                     /locus_tag="VF547_06030"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEX8262414.1"
                     /translation="MILPLLLAAAAAAPQPAEIKTFQDWTVGCDNGAACHAVALMPED
                     WPDDGLTMSVRRGPEAGAQPVLAFELGADSNAASVSADGKRLAVRLVGSEGQTKVAPA
                     DTAAMIAVLRSASRLDLQSADGKPLGQVSLKGASAALLYMDEKQRRTGTATALVRPGT
                     RPASGSPPPLPVVAAAPLSSAPPLALSRAALAALRRKHGCTLDEVGGPEEAETGTMTR
                     TETLLLLACGSGAYNVSYVPFVLRRGRGGVGAELAAFDVKEPWWAEEGKPMLINAGWD
                     KERGLLMSYSKGRGLGDCGTTAAYAWDGRAFRLVEQAEMGECRGSTDYITTWRAKVVR
                     R"
     gene            complement(3667..4629)
                     /gene="egtD"
                     /locus_tag="VF547_06035"
     CDS             complement(3667..4629)
                     /gene="egtD"
                     /locus_tag="VF547_06035"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX8262415.1"
                     /translation="MDAPRRPAADPDFRHDVLAGLAAPIRAVPARWLYDRRGSELFEA
                     ITALPEYYLTRTETALLERRCPDVARIAGRGQAVVEFGSGASVKTRILLAAAEPSAYV
                     PVDISGDFLRDASASLADDFPGLAVFPIEADFTRPFQLPAQLAGAPKLGFFPGSTIGN
                     LVPSSGVDLLRSMRQTLGTGAWLFIGFDRVKDPAELLRAYDDAAGITAEFNCNLLVRI
                     NRELDGGIPLDAFRHRAVWNERLSRIEMHLEAVRDVDFEVSGERFSMAAGETIHTENS
                     HKYGERDARLLLLAGGWTPLEQWTDDDARFALVLARAEPERSAP"
     gene            complement(4777..6075)
                     /gene="egtB"
                     /locus_tag="VF547_06040"
     CDS             complement(4777..6075)
                     /gene="egtB"
                     /locus_tag="VF547_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX8262416.1"
                     /translation="MATQADSPPTAALAERFHAVRSLSEAIAAPLSNADATLQPMPDA
                     SPAKWHHAHTSWFFETFVLRDHVPGHRPYDESWAYLFNSYYDGEGERHPRARRGMLSR
                     PSLAEILDYRAHVDGALAAALPRLPETALALVELGLNHEQQHQELMLMDLTAAFAENP
                     LRPALWAGPPLSPHRPATPLRWVEGRSGIVEIGHSGGGFAFDCEGPRHPVLLHRHALA
                     HRLVSNAEWAGFIEDGGYSRPELWMADGWAWAAGNGIEAPAYWLRGEAGWSRYGLDGT
                     RPLEPDEPVCHISWYEADAFARWAGARLPREEEWEAAAAPLNPAGGEQLDGGDPADTV
                     SRQPWCGSGPQHSKTRSAPQDMFGCRWEWTMSAFAPYPGYRPVEGTVGEYNGKFMCNQ
                     FVLRGGSCATPRGHLRASYRNFFYPHQRWMFAGLRLARDV"
     gene            complement(6199..>6367)
                     /locus_tag="VF547_06045"
     CDS             complement(6199..>6367)
                     /locus_tag="VF547_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEX8262417.1"
                     /translation="VRRAERSAIAASASTPLNPLAPIYLNRLSDHLFVLARLAAAGEG
                     GEVLWKPGATR"
BASE COUNT          972 a         2174 c         2345 g          876 t
ORIGIN      
        1 gccaggtcct gaccttcgtc gatctcggtc cgaggctgat cacggtcacc catcacgacg
       61 cggtcgccgg gccctatcac cgcaacggcc gggacatcat cgacgtgatg cgggccttcc
      121 gaggctccgc ggacgccgct cgcgcgacga tcgagcgccg gcggatcgac tatgtgctga
      181 tctgccccgg cctttccgaa tcgaccatct atgccagcca ggccaagggc ggcttctaca
      241 cccagctcgc gtcgggcaag gcgccggcct ggctggagcc ggtgccgctt ccggaaaagt
      301 cgccctaccg gatgtggcgg gtggtgagag gccgctgatc ccggtcggct ctgcccggcg
      361 tcattgcgag gagcgaagcg acgcggcaat ccagcgggcc tcggcggctg cactggattg
      421 cttcgcttcg ctcgcaatga cggccggccc tagacctcga agctgaccga gatcccgtcg
      481 atgacgaact gggccgcgag cgcggcgagg atcacgccca ggaggcgggt cagcatcgcc
      541 tcggtgcggt ggccgagcag gcgcatcagc cagccggcgg cgagcagcga cgcgagggtg
      601 agcagaagca ccgccgcgag cgcggcgagc acgaccagcg attcgtcgag cccgtcgctg
      661 cgcgccatca gaagcatgat cgaggcgatc gagccggggc cggcgatcat cgggatcgcc
      721 atcgggaaga cggagacgtc gtcatgcccg ggatcggcgc tgacctcctg ggcgcgggtc
      781 tcgcggcgcc gggtgcggcg ctcgaacacc atctcgaggg cgatcaggaa caacatgatg
      841 ccccccgcga tccggaaggc ggcgaggctg atgccgagcg tcctcagcag atcctcgccg
      901 agcagcgcga agaagaggag gatcccggcc gcgaccagcg acgcccggac cgccatcgac
      961 cgccgctcgg cggcggcggc ctcgcgcgtc agtccggcga agatcggcgc gcagccgggc
     1021 gggtcgatga ccacgaagaa ggtcgcgaag gcggagagga agagcgcgat cacaaaccct
     1081 ccggcggagg caggccggcg cgggcgtggg cggcgacgag cgtgttgcgg agcaggacgg
     1141 cgatggtcat cggcccgact ccgcccggga ccggagtgat cgcggcggcg acgtcggagg
     1201 cgctgtcgaa atggacgtcg ccgaccagcc ggggcttgcc ctcgccctcg ggggcgaccc
     1261 ggttgatgcc gacgtcgatc acggtcgcgc cgggcctcag ccagtcgccg cggaccatct
     1321 cggcgcggcc gaccgcggcg acgacgatgt cggcgcggcg gaccaccccg ggcaggccgc
     1381 gggtgcggct gtgggcgatc gtcacggtgc aattctcggc gagcaggagc tgcgccatcg
     1441 gcttgccgac gatgttcgag cggccgatca cgaccgcctc caggccggcg aggtcgccga
     1501 gccggtcctt gagcagcatc aggcagccga gcggcgtgca gggcaccagc ccgccctcgc
     1561 cgctcgccag ccggccggcg ttgaccgggt ggaagccgtc gacgtccttg gccgggtcga
     1621 ccgcctcgat gatcgccttg tcgtcgatcc cggccggcag cggcagctgg acgaggatgc
     1681 cgtcgaccga ctcgtcctcg ttgaggcttt cgaccagcgc gagcagctcc tcctgcgaca
     1741 cgctgtcggg caggcggtgc tcgaagctcg ccatgccggc ctcggtggtc gcccggccct
     1801 tggagcggac atagacctgg ctggccggat cctcgccgac gagcaccacc gccaggccgg
     1861 gggcccgccc gacggcttgg gcgaaggcgg gcgcggcggc ggcgacccgc tcgcgcagcc
     1921 gggcggcgaa ggctcggccg tcgatccgct cggcgctcat cgatagcttg cggcgacgct
     1981 gaaggcgacg tcgtacagga tctggttgag gatccacagc accagcagga ccaccatcgg
     2041 gctgaagtcg aggcctccga aatcgggcag gacccggcgg atcggccggt agaccggctc
     2101 ggtgatgcgg tcgaggccgc gaagcaggcc gcggacgaag tcattgtagg tgttgatgac
     2161 gttgaaggcg accagcaggg acaggatcat ctggatgacg acgatccacc agaccacctt
     2221 gagaaggatg atcacgacgt cgatcagggc cagcagcatt cgtctctctc ccgcgctcgg
     2281 tcacgggcga tgtagggaag cggcggatcg ggggcaatgc cgaccccgcc cgcgccgcaa
     2341 agcttgcgaa acttacacat gccatccggc gctgtcggct tcggccccat cgtccggccg
     2401 gccgcccgcg acgggctggg gacgaaggct ggtcgagtgg gaggcgcaag gcacaagcga
     2461 ttagaacata taatgaacaa actgtcaatg gcatttggcc ggacgcggct ctagcatccg
     2521 cccatgatcc tgcccctgct tctcgccgcc gccgccgcgg cgccgcaacc cgccgagatc
     2581 aagaccttcc aggactggac ggtcggctgc gacaacggcg ccgcctgtca cgccgtggcg
     2641 ctgatgcccg aggactggcc cgacgacggg ctgacgatga gcgtccggcg cgggcccgag
     2701 gcgggcgccc agccggtgct cgcgttcgag ctcggcgccg acagcaacgc cgcctcggtc
     2761 agcgccgacg gcaagcggct cgcggtccgg ctggtcggct ccgaggggca gaccaaggtc
     2821 gccccggccg acaccgcggc gatgatcgcg gtgcttcgct cggcgagccg gctcgatctc
     2881 cagtcggccg acggcaagcc cctcggccag gtgtcgctca agggcgccag cgccgctttg
     2941 ctctacatgg acgagaagca gcgccgcacc ggcacggcga ccgcgctggt ccgtccgggg
     3001 acccgcccgg cgtcgggctc gccgcctccc ctgccggtgg tcgccgcggc gccgctgtcc
     3061 tcggcgccgc ccctcgccct ctctcgggcc gcgctcgccg cccttcgccg caagcatggc
     3121 tgcacgctcg acgaggtcgg cgggcccgag gaggcggaga ccggcacgat gacccggacc
     3181 gagaccctgc ttctcctcgc ctgcggctcg ggcgcctaca atgtcagcta cgtccccttc
     3241 gtgcttcgcc gcggccgcgg cggagtcggc gccgagctcg ccgccttcga cgtcaaggag
     3301 ccctggtggg cggaggaggg caagccgatg ctgatcaacg ccggctggga caaggagcgc
     3361 ggcctgctga tgagctattc gaagggccgc ggcctcggcg attgcggaac caccgccgcc
     3421 tacgcctggg acggccgcgc cttccgcctg gtcgagcagg ccgagatggg cgagtgccgg
     3481 gggtcgaccg actacatcac gacctggcgg gcgaaggtgg ttcggcggta gggacaaaat
     3541 tcctccccgg catttgccgg ggagggtgac cctgcggagc atggtggagg ggttctttcg
     3601 gcgcttgaag aacccctcca ccgcgcttcg cgcggtcccc ctcccccgga aaatccgggg
     3661 gaggaattaa ggggcgcttc gctccggctc ggcccgcgcc agcacgagcg caaacctcgc
     3721 gtcgtcgtcg gtccattgct cgagcggggt ccagccgccg gcgagcagga gaagcctcgc
     3781 gtcgcgctcg ccatatttgt ggctgttctc ggtgtggatg gtctcgcccg ccgccatgga
     3841 gaaccgctcg ccggagacct cgaagtcgac gtcgcgaacg gcctccagat gcatctcgat
     3901 ccggctcagc cgctcgttcc agaccgcgcg gtggcggaag gcgtcgagcg ggatgccgcc
     3961 gtccagctcg cggttgatgc ggacgaggag gttgcagttg aactcggcgg tgatgccggc
     4021 ggcgtcgtca taggcgcgca gcagctcggc gggatccttg acccggtcga agccgatgaa
     4081 cagccaggcg ccggtgccga gcgtctggcg catcgaccga agcaggtcga ctccgctcga
     4141 ggggacgagg ttgccgatcg tcgagcccgg gaagaagccg agcttggggg cgcccgccag
     4201 ttgcgccggc aattggaagg gccgagtgaa atcggcctcg atcggaaaga cggcgaggcc
     4261 cgggaagtcg tcggcgagcg aggccgaggc gtcgcgcagg aagtcgccgc tgatgtcgac
     4321 ggggacgtag gcggacggct cggccgcggc gaggaggatt cgggtcttca cggaagcgcc
     4381 ggagccgaac tcgacgaccg cctggccgcg gccggcgatc cgggcgacgt ccgggcagcg
     4441 gcgctccaga agggcggtct ccgttcgggt gagataatat tcgggaagcg cggtgatcgc
     4501 ctcgaacagc tccgagccgc ggcggtcgta gagccagcgg gcggggacgg cgcggatcgg
     4561 cgcggcgagg ccggcgagca cgtcgtggcg gaaatccgga tcggctgcgg ggcggcgcgg
     4621 cgcgtccatc acgtcaggct ccactgggat cggcttgccc cgctccgttc gggctgagcc
     4681 cgtcgaagcc gattgttcga atccgcaggg cgatcggagg acgagggctt cgacacgctc
     4741 ggcccgaacg gtttgccgcg accgaccgga tccaccctag acatcccggg cgagccggag
     4801 ccccgcgaac atccagcgct gatgcggata gaagaagttg cggtagctgg cgcgcaggtg
     4861 cccgcgcggc gtggcgcagc tgccgcctcg aagcacgaac tggttgcaca tgaacttgcc
     4921 gttatattcg ccgaccgtgc cttcgacggg ccggtagccg ggataggggg cgaaggcgct
     4981 catcgtccat tcccaccggc acccgaacat gtcctggggc gccgaccggg tcttcgaatg
     5041 ctgcgggccg gagccgcacc agggctgcct ggaaacggtg tcggccggat cgccgccgtc
     5101 gagctgctcg cctcccgccg ggttcagcgg cgccgcggcc gcctcccatt cttcctcgcg
     5161 cggcagacgc gccccggccc atcgggcgaa ggcgtcggct tcgtaccagc ttatgtggca
     5221 gaccggctcg tccggctcga gcggccgtgt tccgtcgagg ccgtagcggc tccagcccgc
     5281 ttcgccccgc agccagtaag ccggcgcctc gatgccgttg ccggcggccc aggcccaccc
     5341 gtccgccatc cacagctccg gccgggaata gccgccgtcc tcgatgaagc cggcccattc
     5401 ggcgttgctg accagccggt gggcaagggc gtgccggtgg aggaggacgg ggtggcgcgg
     5461 cccctcgcag tcgaacgcga agccgccgcc ggaatggccg atctcgacga ttccgctgcg
     5521 cccctccacc cagcgaaggg gtgtcgccgg cctgtgcggg gaaagcggcg gcccggccca
     5581 cagcgccggc cgaagcggat tttcggcgaa ggcggcggtc aggtccatca gcatcagctc
     5641 ctgatgctgc tgctcgtggt tgaggccgag ctcgaccagc gccagcgcgg tttccggcag
     5701 gcggggcagc gcggcggcca gggccccgtc gacatgcgcc cgatagtcga ggatctcggc
     5761 gaggctcgga cgcgagagca ttcctcgccg cgcgcgcggg tggcgctcgc cctcgccgtc
     5821 gtaataggag ttgaacagat aggcccagct ttcgtcgtac ggacgatggc ccggcacatg
     5881 gtcgcgaagc acgaaggtct cgaagaacca gctggtgtgc gcgtggtgcc atttggccgg
     5941 cgaggcgtcc ggcatcggct ggagggtggc gtcggcgttc gacagcggcg cggcgatcgc
     6001 ctcgctcagc gaccgcaccg cgtggaagcg ctcggccagc gccgcggtgg gcggggaatc
     6061 ggcctgggtc gccatccgcg ccctcctaca ccaaaatatt gatggaggtt agcatctgtc
     6121 cgccctcccg gcgaaggccc tcgggacgag aagtggcggc aggcctcccc gaggccccgg
     6181 ctttcgccgg gatgacggtc agcgggtcgc tcccggcttc cagagcactt ctcccccctc
     6241 tcccgccgcg gccagccggg ccaggacgaa gaggtggtcc gagaggcggt tcaggtagat
     6301 gggcgcgagg gggttgaggg gggtcgaggc ggaggcggct atggcggagc gctcggcgcg
     6361 ccggacg
//
LOCUS       DASVQZ010000222         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_915101, whole genome shotgun sequence.
ACCESSION   DASVQZ010000222 DASVQZ010000000
VERSION     DASVQZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915101"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1570
                     /locus_tag="VF547_06050"
     CDS             230..1570
                     /locus_tag="VF547_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8262418.1"
                     /translation="MSEVQPAKRAFGFWICLALVIGNMIGSGVFLLPQQLAPYGWNAI
                     LGWGVTIPGALCLAFVFASLARRFPRAGGPYAYTAEAFGAAPGFAVAWSYWISVWTAN
                     AAISLAAVSYLSHFLPGLAAAPGIAAIGLVWTFTLINCLSVKGTGEVQLATTALKLLP
                     LIAAIVLTLTVAATGDRPDAVPLPADEISWAGVTATAALTLWAMVGFESATIPAGHVR
                     DPERTIPRATMIGTLITGLVYLIACSGVALLLPPEQAAASNAPFADFIGRYWGPGPAS
                     LVALFAAISALGALNGWVLIQGELPVALARDGRFPAWFARTSAAGTPVRAHLVSSSLV
                     TLLLLANQSRSMGNLFAFMALLSTAASLVAYLSCSLAALRLQSDGRMPRSFVLPFVAI
                     AGALYSAWAIYGAGAEPALWGAALLASGIPVYLLMRRNLLRRRTVETTSSTTEG"
     gene            1572..1979
                     /gene="msrB"
                     /locus_tag="VF547_06055"
     CDS             1572..1979
                     /gene="msrB"
                     /locus_tag="VF547_06055"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8262419.1"
                     /translation="MADQERDRAQMSEREWRERLSPEQYHVLREAGTEPPFTGRYVNN
                     HEEGEYRCAGCGELLFDSACKFDSGSGWPSFTEPAAQSISEHADMSHGMRRVEVRCAR
                     CDGHLGHLFPDGPGPTGLRYCINSASLDFKARK"
     gene            2065..>3845
                     /locus_tag="VF547_06060"
     CDS             2065..>3845
                     /locus_tag="VF547_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8262420.1"
                     /translation="MASVASPPSRVRRTLITLLKIGLYGGLLALVALVVAVGVAMSSL
                     PSYQELVRRDDLGQTIRVRAADGSILLSSGPSFGNWLRYDEIPDIMKAAMVAVEDKRF
                     RSHLGVDPIGTVRAFKVRIDKGRWTQGGSTITQQLARNIFLTNSRTFGRKVREGVLAL
                     ALERRFSKDQILELYLNRVYFGGGAYGIDAASRRFFGHSAEELSLSEAAIIAGLVKAP
                     SNYSPTADAAAAKGRAGIVLDLMLQQGYITGAEAALAEPAQVALVPVQKQNSVRYFTD
                     WVLPQLDTLIDETVEPIDVWTTLDLGMQRSADQAINANTPAGVQGALVSLDRDGAIRA
                     MVGGRDYVNSIYNRATQATRQPGSAFKLFVYLAALEAGFRPEDSVADEPITINGWTPR
                     NSTRTYSGAVTVREAFARSINTVSARLGQEVGFRTVADMAQRFGITTEVNTHPSMVLG
                     TSDVRLLDMTRAFAAVARKGTAVTPYGIRRVTTADKQILYEHEADESRVLVAPWVAAQ
                     MTDLLQAAVLTGTGRAAQIGRPVAGKTGTTSSNKDGWFIGFSSGITTGVWMGRDNARA
                     IPGLQGGRAPAEAFHDFMVQAVATRPP"
BASE COUNT          546 a         1404 c         1265 g          630 t
ORIGIN      
        1 ggccgggcgt acgaccggca acagctcgat cgcctgctcg ccttcacgcg ccggcaggcc
       61 tcgtcgccgg ccaattgggc caagctcctg tggggattcg cccgaagctc ggtcgcttcc
      121 gacctttgag gacagccctt cccgccgacc gcgacagctc gggcggcgcc ggcgccggag
      181 gcgtcggaat gtcctttgcc cgccgcagca actgcgccat gcttcgcgca tgagcgaagt
      241 ccagcccgcg aagcgcgcct tcgggttctg gatctgcctc gcgctcgtca ttggcaacat
      301 gatcggatcg ggcgtgttcc tgctgcccca gcagctcgca ccttacggct ggaacgcgat
      361 cctgggctgg ggggtgacga tccccggagc gctttgcctc gccttcgtct tcgcaagcct
      421 cgcccgccgc ttcccgcgcg ccggcggccc ctatgcctat accgccgagg ccttcggcgc
      481 cgcgcccggc ttcgccgtgg cctggagcta ctggatctcg gtgtggaccg cgaacgcggc
      541 gatctcgctc gccgcggtca gctatctcag ccacttcctt ccggggctgg cggcggcgcc
      601 cggcatcgcc gcgatcggtc tcgtgtggac cttcaccctg atcaactgcc tcagcgtcaa
      661 gggcacgggc gaggtccagc tcgccaccac cgcgctgaag ctcctcccat tgatcgcggc
      721 gatcgtcctg acgctcaccg tcgccgccac cggcgaccgc cccgacgcgg tgccgctccc
      781 cgccgacgag atcagctggg ccggggtcac cgccaccgcg gccttgacct tgtgggcgat
      841 ggtcggcttc gaatcggcga ccattcccgc cggccacgtc cgcgatccgg aacggaccat
      901 tccccgcgcg acgatgatcg gcacgctgat caccgggctc gtctacctga tcgcctgctc
      961 cggcgtggcg ctgcttctgc ctcccgagca ggcggccgcc tccaacgcgc cgttcgccga
     1021 cttcatcggc cgctactggg gtccggggcc ggcctccctc gtcgccctgt tcgccgcgat
     1081 cagcgcgctc ggcgccctga acggctgggt cctgatccag ggcgagcttc cagtcgcgct
     1141 ggcccgcgac ggccgcttcc cggcctggtt cgccaggacc tccgcggccg ggactccggt
     1201 gcgcgcgcat ctcgtgtcga gcagcctcgt caccttgctg ctgctggcca accagagccg
     1261 ctcgatgggc aatctgttcg ccttcatggc gctgctttcg accgcggcgt cgctggtcgc
     1321 ctatctgagc tgctcgctcg cggccctccg gctgcagtcg gacggtcgaa tgccgcgctc
     1381 cttcgtcctt cccttcgtcg cgatcgcggg agcgctctat tcggcctggg ccatctacgg
     1441 cgccggggcc gagccggcct tgtggggggc ggcgctgctg gcgagcggca tcccggtcta
     1501 cctgctgatg cggcgaaacc tgttgcggcg ccggaccgtt gagacgactt catcgacaac
     1561 cgagggatga gatggcggac caggaacggg atcgtgccca gatgtcggaa cgcgagtggc
     1621 gcgagcggct gagcccggag caatatcacg tgcttcgcga ggctgggacc gagccgccct
     1681 tcaccggccg ctacgtcaac aatcacgagg agggagaata tcgctgcgcc ggctgcggcg
     1741 agctgctgtt cgacagcgcg tgcaagttcg acagcggcag cggctggccc agcttcactg
     1801 agccggcggc gcagagcatc agcgagcatg cggacatgag ccacggcatg cgccgggtcg
     1861 aggtccgctg cgcgcgctgc gacggccatc tcggccacct cttcccggac ggccccgggc
     1921 cgacgggcct gcgctactgc atcaacagcg cctcgcttga tttcaaggcg aggaagtgac
     1981 cgggcggcct gcacggcccg acctccgctt ctccgcccac cctcccttgt cgcccgactc
     2041 gtctgcccct aggctgcggc cccgatggcc agtgtcgctt ctcccccgtc acgcgttcgc
     2101 cggacgctga tcacccttct caagatcggg ctgtacgggg ggcttcttgc cctcgtcgcg
     2161 ctggtcgtcg ccgtcggcgt ggcgatgagc tcgctgccca gctatcagga gctggtccgg
     2221 cgcgacgacc ttggccagac gatccgggtg cgtgccgccg acggatcgat cctgctctcc
     2281 tcgggcccga gcttcggcaa ctggctgcgc tacgacgaga tacccgacat catgaaggcc
     2341 gcgatggtcg cggtcgagga caagcgcttc cgttcccatc tcggagtcga cccgatcgga
     2401 accgtccggg cgttcaaggt ccggatcgac aaggggcgct ggacccaggg cggctcgacc
     2461 atcacgcagc agctcgcccg caacatcttc ctgaccaaca gccggacgtt cgggcgcaag
     2521 gtccgcgaag gcgtgctggc gctggcgctc gagcggcgct tctcgaagga ccagatcctc
     2581 gaactgtatc tcaatcgcgt ctatttcggc ggcggcgcct acggcatcga cgcggcatcg
     2641 cgccgcttct tcggccacag cgccgaggag ctgtcgctct ctgaagccgc gatcatcgcc
     2701 ggcctcgtca aggcgccgtc caattattcg ccgaccgccg atgccgccgc tgccaagggc
     2761 cgggccggca tcgtcctcga tctgatgctg cagcagggct atatcaccgg cgcggaggcg
     2821 gcgcttgccg agccggcgca ggtcgcgctc gtgcccgtgc agaagcagaa cagcgtgcgc
     2881 tacttcaccg actgggtgct gccgcagctc gacacgctga tcgacgagac ggtcgagccg
     2941 atcgacgtct ggaccacgct cgatctcggc atgcagcgct cggccgatca ggcgatcaac
     3001 gccaacaccc cggccggagt gcagggcgcc ctggtctcgc tcgaccgcga cggagctatc
     3061 cgggcgatgg tgggcgggcg cgactatgtg aactcgatct acaaccgcgc gacgcaggcc
     3121 actcgtcagc cgggctcggc cttcaagctg ttcgtttacc tcgcagcgct ggaagcgggc
     3181 ttcaggccgg aggacagcgt cgccgacgag ccgatcacga tcaacggctg gacgccgcgc
     3241 aacagcaccc gcacctattc gggcgcggtg acggtgcggg aggccttcgc gcgctcgatc
     3301 aacaccgttt cggcgcggct cgggcaggaa gtcggcttcc gcaccgtcgc cgacatggcc
     3361 cagcgcttcg gcatcaccac cgaggtcaac acacacccct cgatggtgct cggcacgtcg
     3421 gacgttcgcc tgctcgacat gacccgtgcc tttgccgctg tcgcgcgcaa gggcacggcc
     3481 gtgacgccat acggcatccg ccgcgtcacc acggccgaca agcagatcct gtacgagcat
     3541 gaagcggacg aatcgcgggt gctggtcgcg ccctgggtcg ccgcgcagat gaccgacctg
     3601 ctgcaggcgg cggtgctgac cgggaccgga cgtgccgcgc agatcggccg cccggtcgcc
     3661 ggcaagaccg gcaccaccag ctcgaacaag gacggctggt tcatcggctt ttcgagcggg
     3721 atcaccaccg gcgtgtggat gggccgcgac aatgcccggg cgataccggg ccttcagggg
     3781 ggccgcgcgc cggccgaggc attccacgac ttcatggtgc aggcggttgc cacccgcccg
     3841 cccga
//
LOCUS       DASVQZ010000223         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_47086, whole genome shotgun sequence.
ACCESSION   DASVQZ010000223 DASVQZ010000000
VERSION     DASVQZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47086"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..511
                     /gene="aroQ"
                     /locus_tag="VF547_06065"
     CDS             71..511
                     /gene="aroQ"
                     /locus_tag="VF547_06065"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX8262421.1"
                     /translation="MAALPTIYVLNGPNLNLLGTREPEIYGSGTLDELAGQIEDRALE
                     LGVEVEIRQSNHEGHLIDWLHEAQARGAKAVILNAAGFTHSSVALHDAVKALSTPVIE
                     VHLSNPQAREGFRRRSLIAAAAKGSIAGFGPLSYLLALDAAARL"
     gene            565..1044
                     /gene="accB"
                     /locus_tag="VF547_06070"
     CDS             565..1044
                     /gene="accB"
                     /locus_tag="VF547_06070"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX8262422.1"
                     /translation="MSDEDGASNGGMRIEADLVRQLAELLNQNDLTEIEVEDGDRKIV
                     VKRSFPVAAAVPAPAPAAAASEPAAAPPAAAPTSPASHPGAVKSPMVGTAFLAGEPGA
                     RPFVAPGQKVKEGDTLLIIEAMKVMNPIAAPRAGTVAQVLVVDAQPVEYDQPLVILE"
     gene            1154..2515
                     /gene="accC"
                     /locus_tag="VF547_06075"
     CDS             1154..2515
                     /gene="accC"
                     /locus_tag="VF547_06075"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8262423.1"
                     /translation="MMIKKVLIANRGEIALRIHRACHEMGIKTVAVHSTADTEAMHVR
                     LADEAVCIGPPAASESYLNIPAIISAAEISHADAIHPGYGFLSENARFAEIVELHNII
                     WIGPKPEHIRVMGDKVEAKRTAAKLGLPLVPGSDGPVEGPEEARAVAAETGYPLLVKA
                     ASGGGGRGMKIVRDEEELDSQLSQARSEAKNAFGDDTVYIEKYLGDPRHIEFQVFGDG
                     KGHAVHLGERDCSLQRRHQKVLEEAGSPVITPEERDRMGGIVAKAMAEMGYRGAGTIE
                     FLYENGEFFFIEMNTRLQVEHPVTEYITGIDLVREQIRIAAGEPLSCTQDQVQFRGHA
                     IECRINAEDPETFAPSPGTVKGFHAPGGMHVRVDSGLYVGYKVPPYYDSMIAKLIVYG
                     STRERCIMRLRRALEEMVIDGVKTTIPLHQKLVNEPDFLAGNYTIKWLEAWLARAEQP
                     SSP"
     gene            2571..2912
                     /locus_tag="VF547_06080"
     CDS             2571..2912
                     /locus_tag="VF547_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262424.1"
                     /translation="MTDSRFQFSVAPEKLWQAINPWTLMNQGGQFGLVNISLGQTPRP
                     DIEQKVLDEVGSYGRQLGWIGDALQVLIDHVKLDGLTPAESDALTILKGQLAAIRKVK
                     QRERADEGSSE"
     gene            2909..3244
                     /locus_tag="VF547_06085"
     CDS             2909..3244
                     /locus_tag="VF547_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEX8262425.1"
                     /translation="MKATIWHNPNCGTSRKTLEILRESGADVTVIEYLKTPPTRDELR
                     RIYERAGLAPAEGLRPKEEGASDVAEAGDEAVLDAMIGNPMLIERPLVETEKGVRLCR
                     PQDKVREIL"
     gene            3377..4111
                     /gene="aat"
                     /locus_tag="VF547_06090"
     CDS             3377..4111
                     /gene="aat"
                     /locus_tag="VF547_06090"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX8262426.1"
                     /translation="MAAIDPDLLLRAYAIGVFPMSDSRDADEVFWVEPRRRAILPLDR
                     FRLSRSLRKTIRSGEYRVTCDTAFAEVVRRCAEREETWINADIEESYRLLHERGHAHS
                     IECWHEDALAGGLYGVNLGAAFFGESMFSARRDASKVALAWLVARLRVGGYRLLDCQF
                     MTPHLRSLGAVEVTQKDYLALLESALSAAVPDGSGVATDASGAERRGAGAEFRALDRL
                     LAADAARTVDSPASGWVIAQLLGQTS"
     gene            complement(4147..4542)
                     /locus_tag="VF547_06095"
                     /pseudo
     CDS             complement(4147..4542)
                     /locus_tag="VF547_06095"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
BASE COUNT          758 a         1591 c         1549 g          661 t
ORIGIN      
        1 ccgtcatcgt cccacctgtt ccggcacctg attgtgcgcg tcatccgatc gtcatatagc
       61 gccgcccgca atggcggccc tccccaccat ctatgtactg aacggaccca atctcaacct
      121 gctcggcacg cgcgagccgg agatctacgg cagcggcacg ctcgacgagc tcgcggggca
      181 gatcgaggat cgcgcgctcg agctcggagt cgaggtcgag atccgccagt ccaaccatga
      241 aggccatctg atcgactggc tgcacgaggc gcaggcgcgg ggcgccaaag cggtgatcct
      301 caacgccgcc ggcttcaccc acagctcggt ggccctgcac gacgccgtga aggccctctc
      361 gacccccgtg atcgaggtcc acctctccaa cccccaggcg cgcgagggct tccgccggcg
      421 cagcctgatc gcggccgcgg ccaagggcag catcgccggc ttcgggccgc tgtcctatct
      481 tctggctctg gacgcggcgg cccgtctctg acaggaagcc gcggttcgac gggcttcgtc
      541 ccggaacagg gaaagagact ccagatgagc gatgaagacg gcgcctcgaa cggcggcatg
      601 cggatcgaag ccgatctggt gcgccagctg gccgagctcc tcaaccagaa cgacctgacc
      661 gagatcgagg tggaggacgg cgaccgcaag atcgtcgtca agcgcagctt ccccgtcgcc
      721 gccgccgtcc cggctccggc gccggcggcc gccgcttcgg agccggccgc ggccccgccc
      781 gcggcggcgc cgacgtcgcc ggccagccat ccgggggcgg tcaagtcgcc gatggtcggc
      841 accgccttcc tcgccgggga gccgggcgcg aggccgttcg tcgccccggg gcagaaggtc
      901 aaggaaggcg acaccctgct gatcatcgag gcgatgaagg tgatgaaccc gatcgccgcg
      961 ccgcgggccg gcacggtcgc gcaggtgctc gtcgtggacg cccagccggt ggaatatgac
     1021 cagccgctgg tgatcctgga atagagatga gcgcgccgct tccgtcatcc cagcgaaagc
     1081 tgggatccat gccgaccttg agcccgctgc atggatattc tgcatggacc ccagccttcg
     1141 ctgggctgac gagatcatga tcaagaaggt cctcatcgcg aaccggggcg agatcgcgct
     1201 gcggatccac cgcgcctgcc acgaaatggg catcaagacc gtcgccgtcc attcgactgc
     1261 ggacacggag gcgatgcatg tgcgcctcgc cgacgaggcg gtgtgcatcg gcccgccggc
     1321 ggcttccgaa tcctatctca acatcccggc gatcatctct gccgccgaga tcagccacgc
     1381 cgacgcgatc catcccggct acggcttcct ctccgagaac gcccgcttcg cggagatcgt
     1441 cgagctccac aacatcatct ggatcgggcc caagcccgag catatccggg tgatgggcga
     1501 caaggtcgag gccaagcgca ccgccgccaa gctcgggctg ccgctcgtgc cgggttcgga
     1561 cgggccggtc gaaggtccgg aggaggcgcg cgccgtcgcc gccgagaccg gctacccgct
     1621 gctcgtcaag gccgcttcgg gcggcggcgg gcgcgggatg aagatcgtgc gcgacgagga
     1681 ggagctcgac agccagctca gccaggcgcg ctccgaggcg aagaacgcct tcggcgacga
     1741 caccgtctat atcgagaaat atctcggcga cccgaggcac atcgagttcc aggtgttcgg
     1801 cgacggcaag ggccacgccg tccacctcgg cgagcgcgac tgctcgctgc agcgccggca
     1861 tcagaaggtg ctcgaggaag cgggctcgcc ggtcatcacg cccgaggagc gcgaccgcat
     1921 gggcggcatc gtcgccaagg cgatggccga gatgggctat cgcggcgccg gcacgatcga
     1981 gttcctctac gagaacggcg agttcttctt catcgagatg aacacccggc tgcaggtcga
     2041 gcatccggtc accgaatata tcaccggcat cgacctcgtc cgggagcaga tcaggatcgc
     2101 ggccggcgag ccgctctcct gcacccagga ccaggtccag ttccgcggcc acgcgatcga
     2161 atgccggatc aacgccgagg atccggagac attcgccccc tcccccggca ccgtcaaagg
     2221 cttccacgcg ccgggcggga tgcacgtccg ggtcgatagc ggcctctacg tcggctacaa
     2281 ggtgccgcct tattacgact ccatgatcgc caagctgatc gtctacggct ccacccgcga
     2341 gcgctgcatc atgcgcctga ggcgcgcgct cgaggagatg gtcatcgacg gcgtcaagac
     2401 gaccatcccc ctccatcaga agctggtcaa cgagccggac ttccttgcgg gcaactacac
     2461 gatcaaatgg ctggaggcgt ggctggcccg ggcggagcag ccgagctcgc cttaagggcg
     2521 ccgccccttc cacgcgactt gcgaatcgcc ggcataaccg gaggctttga atgaccgaca
     2581 gcaggttcca attctccgtc gcccccgaga agctctggca ggcgatcaat ccctggacgc
     2641 tgatgaacca gggcgggcag ttcgggctcg tcaacatcag cctcggccag acgccccgcc
     2701 ccgacatcga gcagaaggtg ctcgacgagg tcggcagcta cgggcgccag ctcggctgga
     2761 tcggcgatgc gctccaggtt ctgatcgacc atgtgaagct cgacggcctc accccggccg
     2821 agagcgacgc gctgacgatc ctcaagggcc agctcgccgc gatccgcaag gtgaagcagc
     2881 gcgaacgggc ggacgaaggg agctcggaat gaaggcgacg atctggcaca accccaattg
     2941 cggcacgtcg cgcaagacgc tcgagatcct gcgcgagagc ggcgccgacg tgaccgtgat
     3001 cgagtatctg aagaccccgc cgacccgcga cgagctgcgc cggatttacg agcgcgccgg
     3061 cctcgcaccc gcggagggcc tcaggcccaa ggaggagggg gcctcggacg tggcggaggc
     3121 gggcgacgag gccgtgctcg acgcgatgat cggcaacccg atgctgatcg agcggcccct
     3181 ggtcgagacc gagaagggcg tccgactgtg ccggccgcag gacaaggtcc gggaaatcct
     3241 ctgagaccct tcaccggtcc gcgctgagcc ggtcggcgga ccgtcccgtc cagcggaccg
     3301 ggggaggcgg cttcagagag gctccgcgca aacgggaatg gctacattct accggccaat
     3361 gttctaaggc actccgatgg cggcgatcga tccggacctc ctgctgcgcg cctatgcgat
     3421 cggcgtgttc cccatgtccg acagccgcga cgccgacgag gtcttctggg tcgagccgcg
     3481 gcggcgggcg atccttccgc tcgaccgctt ccgcctgtcg cgctcgcttc gcaagacgat
     3541 ccggtcgggc gagtatcggg tcacctgcga caccgccttc gccgaggtcg tgcggcgctg
     3601 cgccgagcgc gaggagacct ggatcaacgc cgacatcgag gagagctacc ggctgctcca
     3661 cgagcgcggc catgcccatt cgatcgaatg ctggcacgag gacgccctcg ccggcggcct
     3721 ctacggggtc aatctggggg ccgccttctt cggcgaaagc atgttttcgg cccgccggga
     3781 cgcttccaag gtcgcgctcg cctggttggt cgccaggctg agggtcggcg gctaccggct
     3841 gctcgactgc cagttcatga ccccccacct gcgcagcctc ggcgccgtcg aggtgaccca
     3901 gaaggattat ctggcgctgt tggaatcggc gctgtcggcg gcggtgccgg acggctcggg
     3961 cgtggcgacg gacgcttcgg gcgcagagcg ccgcggcgcc ggcgccgagt tccgcgcgct
     4021 cgaccggctg cttgccgccg acgccgcccg gaccgtcgat tcccccgctt ccgggtgggt
     4081 catcgcgcag ctcttgggcc agacgtcgta gatcgggtgc tcgacgacgt tcagcgacgg
     4141 cgattccttg tagagccagc ccgagaagac gcgctcccac cggccctgcg ggctccgcac
     4201 tccgagctgg acgaaggcgc cggtgagctg ctgcacctcc cacggcgcgg tggtctcgca
     4261 ggcgcgcagc cggaccacgg cgtcgccgat tcggaccgcc tggcccggct tgagggtcag
     4321 atcgcgggag atgccgttgc gcttgttgag aaagccgagc acggccacgc gctgcgccat
     4381 cggcgtggtg ccggccgcca ccgcggtcac ggtctgcggg atgtcgacgg tccgtggctc
     4441 gccggccggc tgggcgggcg cgcccgcggc cttctcggcg ccccggtcgc agccggcgag
     4501 ggcggcaagg gcgataagcg cgacgccgag cgggagcctc atgcctggtc cggcgtcca
//
LOCUS       DASVQZ010000224         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_527979, whole genome shotgun sequence.
ACCESSION   DASVQZ010000224 DASVQZ010000000
VERSION     DASVQZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527979"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..834
                     /locus_tag="VF547_06100"
     CDS             130..834
                     /locus_tag="VF547_06100"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX8262427.1"
                     /translation="MTRTLTLALLCAAAATAPAGAAERSYSVADFDRVQVDGPYRVTL
                     STGGYSAARAEGSTQALDRVSIDVQSGTLRIRPNRSAWGGYPGEAAGPVSVALTTRDL
                     RAAAVVGSGSLAVDRAKGLRVDLSVNGSGRLSVASVAADNLVVGLLGGGRITLAGSAK
                     QLKATVQGSGDLHAAELRADDAQILSDTAGNVTLGVARSVKVTSTGAGDVEILGTPSC
                     TVEAKGSGAVLCGPGR"
     gene            complement(831..1235)
                     /locus_tag="VF547_06105"
     CDS             complement(831..1235)
                     /locus_tag="VF547_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEX8262428.1"
                     /translation="MPLHISKVAVGCASLDSLRARQALRLADGIVPVVTRFRPKRADE
                     LVGGSIYWIVKHRIAARQTIVGFAEREQDRRTVIRLDPELVPVRALPRRAHQGWRYLA
                     AEEAPLDLAGDQTGLAELPPELTARLAALALI"
     gene            complement(1469..2869)
                     /gene="mgtE"
                     /locus_tag="VF547_06110"
     CDS             complement(1469..2869)
                     /gene="mgtE"
                     /locus_tag="VF547_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX8262429.1"
                     /translation="MSESEIDLPGAPAGRLDEDDRLKPEYVREVMERVAAGDADGARG
                     LVEPLHPADLADLFELVDSDDRRALAAAIADMLDGDVLAEMNEHVREELVEALDPRQV
                     ADLAGEMETDDAVAMIEDLEEDEQRAVLRELDPDDRAAIEEALAYPEESAGRLMQRDL
                     IAVPEHWTVGQVIDYLRAGEDLTTDFWEVFVVDERHHPVGTCLLSWILRTHRHVAVAD
                     VMKREQTLIPVDMDQEEVALRFQKYALISAAVVDESGRLVGMITVDDIVHIIQEEASE
                     DVLLLSGAGEGDINQPVLESYSSRVRWLIANLGTALVAATIIAQFEGTISRMVALAAL
                     MPIVAGVGGNAGTQTLAVTVRALATNQLTQSNTWRAILREIRVACLNGITVAAVIGVG
                     VGLVFANSGLGAVIAAAMLANIIIAGFAGVFVPLALERAGADPAVASSVFVTMVTDSM
                     GFLVFLGLATASGIAG"
BASE COUNT          426 a         1020 c         1005 g          447 t
ORIGIN      
        1 aacgtcagca agatgggctc gggcgacgcc cgctgcggct cctaggggcg aggcgcgagg
       61 gagggccggt cttgcgcctt ctcgacggcc tcattgaccg ttaaccattt ttcgtcaggg
      121 tccgcggtca tgacccggac cctgaccctc gctctcctgt gcgccgccgc cgcgacggct
      181 cccgccggcg ccgctgaacg cagctattcg gtggccgact tcgaccgcgt gcaggtcgac
      241 ggaccctatc gggtgaccct gtccaccggc ggctattcgg ccgcccgggc cgagggctcg
      301 acccaggccc tcgaccgagt ctcgatcgat gtccagagcg gcacgcttcg gatccgtccc
      361 aaccgctcgg cctggggcgg ctatcccggc gaggcggctg gaccggtcag cgtcgcgctc
      421 accacccgcg accttcgggc cgccgcggtg gtcggttccg gcagcctcgc ggtcgaccgc
      481 gccaagggac ttcgagtcga tctttcggtc aacggaagcg gccgcctgtc ggtggcctcg
      541 gtcgcggccg acaatctagt cgtcggcctg ctcggcggcg gtcgaatcac cctcgccgga
      601 agcgccaagc agctcaaggc gaccgtccag ggcagcggcg acctccatgc ggccgagctg
      661 cgcgccgacg acgcccagat cctgtcggac acggcgggca acgtcactct cggagtggcg
      721 cgctccgtga aggtgacctc gaccggggcg ggcgacgtcg agatcctcgg cacaccgagc
      781 tgcacggtcg aggcgaaggg gtccggcgcg gtcctctgcg gtcccggccg ctagatcaag
      841 gcgagcgccg ccaggcgcgc ggtcagctcg ggcggaagct cggccagccc cgtctggtcg
      901 ccggcgaggt cgagcggcgc ctcctccgcg gcgagatagc gccagccctg atgggcgcgg
      961 cgcggcaggg cgcggaccgg caccagctcc ggatcgaggc ggatgaccgt ccggcggtcc
     1021 tgctcgcgct cggcgaatcc gacgatggtc tgccgggcgg cgatccggtg cttgacgatc
     1081 cagtagatcg agcctccgac cagctcgtcg gcgcgcttcg ggcggaagcg ggtgaccacc
     1141 ggcacgattc cgtcggcgag ccgaagcgcc tgccgggcgc gcagcgaatc gaggctggcg
     1201 cagccgacgg cgaccttgct gatgtgaagc ggcatggaat ttgccttaat tcgataaagg
     1261 gttggggcaa tagggatatc gccgcttaat tcctccccgc gatttcgcgg ggagggggac
     1321 cgcgtccttc gacgccgcct cggcgtcgct caggataaac tgcgcctttg gcgcagcgcg
     1381 gtggaggggt ccttcggatt cggcaaggcc cctccaccat gcttcgcatg gtccccctcc
     1441 cctgcaaatg caggggagga ccttgggatc agccggcgat gccggaggcg gtggcgaggc
     1501 cgaggaagac caggaacccc atcgaatcgg tgaccatggt cacgaacacg ctcgacgcca
     1561 ccgccggatc cgcgcccgcc cgctccagcg cgagcggcac gaacacgccc gcgaagccgg
     1621 cgatgatgat gttggcgagc atcgccgcgg cgatcaccgc gcccaggccg gaattggcga
     1681 agaccagtcc gaccccgact ccgatcaccg ccgcgacggt gatcccgttg aggcaggcga
     1741 cccggatctc gcgcaaaatc gcgcgccacg tgttcgactg ggtgagctgg ttggtggcga
     1801 gcgcgcgcac cgtcaccgcc agcgtctggg tgccggcatt gccgccgact cccgcgacga
     1861 tcggcatcaa ggcggccagc gccaccatcc tggagatcgt tccttcgaac tgggcaatga
     1921 ttgtggcggc gacgagggcg gtgccgagat tggcgatcag ccagcgcacc cggctcgaat
     1981 agctttcgag gaccggctgg ttgatgtcgc cttcgccggc gcccgagagg agaaggacgt
     2041 cctcgctcgc ctcctcctgg atgatgtgga cgatgtcgtc gacggtgatc atcccgacca
     2101 gccggcccga ctcgtcgacc accgcggccg agatcagcgc atatttctgg aagcggagcg
     2161 ccacctcctc ctggtccatg tcgaccggga tcagggtctg ctcgcgcttc atgacgtcgg
     2221 caacggcgac atggcggtgg gtgcgcagga tccagctcag caggcaggtg ccgaccggat
     2281 ggtggcgctc gtcgacgacg aacacctccc agaaatcggt ggtcaggtcc tcgccggcgc
     2341 gcagatagtc gatcacctgg ccgacggtcc aatgctcggg caccgcgatc aggtcgcgct
     2401 gcatcaggcg cccggccgat tcctcgggat aggcgagcgc ctcctcgatc gcggcgcggt
     2461 cgtcgggatc gagctcgcgc agcacggcgc gctgctcgtc ctcctcgagg tcctcgatca
     2521 tggcgacggc gtcgtccgtc tccatctcgc cggcgaggtc ggcgacctgc ctcggatcga
     2581 gcgcctcgac cagctcctcg cggacatgct cgttcatctc ggcgagcacg tcgccgtcga
     2641 gcatgtcggc gatcgccgcg gccagcgccc ggcggtcgtc gctgtcgacc agctcgaaca
     2701 gatcggcgag gtcggcggga tggagcggct cgaccaggcc gcgggcgccg tcggcgtcgc
     2761 cggcggcgac gcgctccatc acctcgcgga catattccgg cttcagccgg tcgtcctcgt
     2821 cgagccggcc cgcgggcgct ccgggcagat cgatttcgct ctcgctcatc cgcccctctc
     2881 cacgccggtg ttgtcgcc
//
LOCUS       DASVQZ010000225         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_915553, whole genome shotgun sequence.
ACCESSION   DASVQZ010000225 DASVQZ010000000
VERSION     DASVQZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915553"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..807)
                     /gene="gloB"
                     /locus_tag="VF547_06115"
     CDS             complement(79..807)
                     /gene="gloB"
                     /locus_tag="VF547_06115"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX8262430.1"
                     /translation="MLDIVRIPVLSDNYVWLAHEPGSGETAVIDPAVAGPVLAEAEAR
                     GWRITQIWNTHWHPDHTGGNEAIKAATGAFVTGPAAEAARIPTLDRRVREGDKVALGP
                     VEADVLEVPAHTAGHIAYHLPSEETIFVGDTLFAMGCGRLFEGSAEQMHANLQRLAAL
                     PPATRAYCAHEYTLSNGRFAVTVEPDNPALAARMIEVETARAAGEATVPTTIGQELAT
                     NPFMRASSAAELAERRRAKDNFRS"
     gene            complement(817..1893)
                     /locus_tag="VF547_06120"
     CDS             complement(817..1893)
                     /locus_tag="VF547_06120"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8262431.1"
                     /translation="MTAAGWPDRRFEALLGSRHPILLAPMAGAGGVELAAGAMEGGAV
                     GSLPCAMLAPDEVLAQVGQVRARAAGPINLNFFCHAVPDRSDERPWRALLAPFRERLG
                     VVAEPSGGPVRRPFDEAMCRVVEATRPELTSFHFGLPEEPLLDRVRASGAKVIASATT
                     VAEARWLAERGVDAIIAQGWEAGGHAGRFLGGRPDEQMGLFALLPQVADSVGCPVIAA
                     GGIADGRGIAAALTLGAAAVQIGSAYLHCPESLIAPAHRAALATEAAEHSVFTNLFSG
                     GLARGLWTAPMSELGPIRPEAPAFPLAAAALAPLAVAAAEKGESGFRPMWAGQAARLG
                     KPLPARDLTEALARDALARIGASV"
     gene            complement(1890..2306)
                     /locus_tag="VF547_06125"
     CDS             complement(1890..2306)
                     /locus_tag="VF547_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8262432.1"
                     /translation="MKYLHTMIRVSDPEETLRFFELLGLRELRRFDNEAGRFTLIFLA
                     APGDEEAQVELTHNWDEQGYSEGRNFGHLAYRVDDIYESCRRLMEGGVTINRPPRDGR
                     MAFVRSPDNISIELLQAGAPLPPEEPWASMPNTGAW"
     gene            2379..>2566
                     /locus_tag="VF547_06130"
     CDS             2379..>2566
                     /locus_tag="VF547_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262433.1"
                     /translation="MKWIVPIVALGLVGAAQVPWDWGDSKDEWASSGDFDKSKALCRK
                     LRNREPPESDRPRPEAAAS"
BASE COUNT          373 a          923 c          914 g          356 t
ORIGIN      
        1 caggctgaag cgaaaagaag gagaaatctc atccctggcc tcctggggtt tcaacgcccc
       61 gatgcgccgg gcggtccctc agctccggaa attgtccttg gcccggcggc gctcagcaag
      121 ctctgccgcc gatgaagcgc gcatgaacgg gttggtcgcc agctcctggc cgatcgtcgt
      181 cgggactgtc gcctcgcccg cggcgcgggc cgtctcgacc tcgatcatcc gcgccgcgag
      241 cgccgggttg tcgggctcga cggtcaccgc gaagcggccg ttcgagaggg tgtattcgtg
      301 ggcgcaatag gcccgagtcg ccggcggcag cgccgccagc cgctggaggt tggcgtgcat
      361 ctgctcggcg ctgccctcga acaggcgtcc gcaacccatg gcgaacaggg tgtcgccgac
      421 gaagatcgtc tcctccgagg gcagatgata ggcgatgtgc ccggccgtgt gcgccgggac
      481 ctcgagcacg tccgcctcga ctgggccgag cgccaccttg tcgccctccc ggacccggcg
      541 gtcgagcgtc gggatccgcg ccgcctcggc ggccggaccg gtgacgaacg cgcccgtcgc
      601 ggccttgatc gcctcgttgc cgccggtgtg gtcggggtgc cagtgggtgt tccagatctg
      661 ggtgatccgc cagccgcgcg cctccgcctc ggccagcacc ggtcccgcga cggcgggatc
      721 gatcaccgcg gtctcgccgg agcccggctc atgggccagc cagacataat tgtcgctgag
      781 caccgggatt cggacgatgt cgagcatggc cccagcctag acgctcgcgc cgatccgcgc
      841 cagcgcgtcg cgggcgaggg cctccgtcag atcgcgcgcg ggaagcggct tgcccagccg
      901 cgccgcctgt ccggcccaca tcgggcggaa gccggattcg cccttctccg ccgcggcgac
      961 ggcgagcggc gccagtgccg ccgcggccag cgggaaggct ggggcctccg gacggatcgg
     1021 gccgagctca gacatcggcg cggtccacag cccgcgggcg aggccgccgc tgaacaggtt
     1081 ggtgaagacg ctgtgttccg ccgcctccgt cgccagagcg gcgcgatggg cgggggcgat
     1141 caggctctcg ggacaatgga gatatgcgga gccgatctgg accgcggcgg cgcccagagt
     1201 aagcgctgcg gcgatcccgc gcccgtcggc gatcccgccg gcggcgatca ccggacagcc
     1261 gaccgaatcg gcgacctgcg gaagaagcgc gaacagcccc atctgctcgt ccggccgccc
     1321 gccgaggaag cggccggcat ggccgcccgc ctcccagccc tgggcgatga tcgcgtccac
     1381 cccccgctcg gccagccagc gggcctccgc gacggtcgtg gcgcttgcga tcaccttcgc
     1441 tccggaggcc cgcaccctgt cgagcagcgg ctcttccgga aggccgaaat ggaagctggt
     1501 cagttccgga cgagtcgcct cgaccaccct gcacatggcc tcgtcgaacg gccgacggac
     1561 cgggcctccg gacggctccg ccaccacccc caaacgctcg cggaacggcg cgagaagcgc
     1621 ccgccacggc cgttcgtccg atcggtcggg cacggcgtgg cagaagaagt tgaggttgat
     1681 cggtccggcc gctcgggcgc gcacctgccc gacctgcgcg agcacctcgt ccggagcgag
     1741 catggcgcag ggcagcgacc cgaccgcgcc gccctccatg gctcccgcgg cgagctcgac
     1801 cccgccggcc ccggccatcg gcgccagcag gatcggatgg cggctgccga gcagcgcctc
     1861 gaagcgccgg tccggccagc cggccgccgt caccaggcgc ccgtgttcgg catcgaagcc
     1921 cagggttcct cgggcggcag cggcgcgccc gcctggagca gctcgatgga gatgttgtcc
     1981 gggcttcgaa cgaaggccat gcggccgtcg cgcggcggcc ggttgatcgt gacgccgccc
     2041 tccatcagcc gccggcagct ctcgtagatg tcgtcgacgc gataggcgag atggccgaaa
     2101 ttccgcccct ccgaatagcc ttgctcgtcc caattgtggg tgagctcgac ctgggcctcc
     2161 tcgtcgcccg gcgcggcgag gaagatgagg gtgaagcgcc ccgcctcatt gtcgaagcgc
     2221 ctgagctcgc gcaggccaag cagctcgaag aagcggagcg tctcctcggg atcggaaact
     2281 cggatcatgg tgtgcaaata tttcatctgg gtcgcggcct ttcggggcgc ctataaggga
     2341 gcggggaggc ccgttccact gcgcgcgcga ggatgccgat gaaatggatt gtgccgattg
     2401 tcgccctcgg cctcgtcggc gccgcccagg tgccgtggga ctggggcgac tcgaaggacg
     2461 aatgggccag ctccggcgac ttcgacaagt ccaaggcgct ctgccggaag ctgcggaacc
     2521 gtgagccgcc ggagtcggac cggccaaggc ccgaggcggc cgcctc
//
LOCUS       DASVQZ010000226         4867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_722990, whole genome shotgun sequence.
ACCESSION   DASVQZ010000226 DASVQZ010000000
VERSION     DASVQZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722990"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VF547_06135"
     CDS             <1..809
                     /locus_tag="VF547_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX8262434.1"
                     /translation="APDRGSAASSFLGAFTILLREGLEALLIVVAMLAFLRKADRAEM
                     VRPVHWGWIAALIAGVATWWAATHLVSISGASRELTEGFGSILAAVILLFVGIWMHGK
                     AQADEWQRYIREKVGQALSRKSAWFLFLLAFIAVYREVFETILFFAALSAEGRLAPLL
                     GGAAAAAALLGLIAVLMLRYSRKLPIAKFFTYSSALIAMLAVVLAGKGVAALQEAGMI
                     GVTPLGSAPRVEILGLYPTVEGLLAQLAALAVLLAGFGRNRRGANRLATT"
     gene            879..1370
                     /gene="ssb"
                     /locus_tag="VF547_06140"
     CDS             879..1370
                     /gene="ssb"
                     /locus_tag="VF547_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8262435.1"
                     /translation="MAGVNKVILVGNLGDDPESRSLNNGGEVVNLRVATSENWKDKDG
                     NRQERTEWHRVVIFNENLGRVAKSYLRKGSKVYLEGQLQTRKWTDQSGADRYSTEVVL
                     QRFRGELVLLDSRGGGGGGGGEDFGGGYESGGSSGFGGGGSRPQSRPAPAFDSDLDDD
                     VPF"
     gene            complement(1405..1926)
                     /locus_tag="VF547_06145"
     CDS             complement(1405..1926)
                     /locus_tag="VF547_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX8262436.1"
                     /translation="MTEFSRLFRLDALAREPRRVRIEAEPDERDALAARFELVSVDAL
                     AAEAELGRHGETVIAAGFLRARVTQSCIATAEPVEAAVEEEFRVEFRPLPAGGDAEEE
                     VELVEDELDVIFYDGSQVDLGEAVAQTLALSLDPYPRSPAAEAALREAGVQDEAEARA
                     ASSPFAVLKALKK"
     gene            complement(1923..2609)
                     /locus_tag="VF547_06150"
     CDS             complement(1923..2609)
                     /locus_tag="VF547_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX8262437.1"
                     /translation="MPLFVPKPSYWKNSRKPAGAGFERRRLPCRQGQALQYPRKAERR
                     KYAPRSRRPALAFLSRLFGARRERAALRPLYDALVAAARDPAWYREGGVPDTLEGRFD
                     MVATMVALVLLRLEAEGDRARAESVQLTEIFIDDMDGTLRQIGIGDFVVGKHVGKLVG
                     ALGGRLGVLREALGDGAPLEAAVRRNVFRDAPAGDAQVAWVAERLGRFHRALGATGME
                     PLLAGSLPDA"
     gene            2585..3058
                     /locus_tag="VF547_06155"
     CDS             2585..3058
                     /locus_tag="VF547_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX8262438.1"
                     /translation="MVSGRKAAVLAAVAAGLALGGCAKTRDIQGYLVDETLVAAVQPG
                     IDNKESVQNTLGRPTFTGTFDQNDWYYVSRRTKNYAYSHPKVNEQTVLHVRFDAAGNV
                     AAVQRKGLEQVVSIDPMHDKTPTLGRNKSIWDELFGNIGAVGAPVGSPGGPNSPN"
     gene            complement(3095..>4867)
                     /locus_tag="VF547_06160"
     CDS             complement(3095..>4867)
                     /locus_tag="VF547_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX8262439.1"
                     /translation="HDDQHGTAIITAAGLINACYLTGRALTDIQVVVNGAGAAAIACT
                     ELIKAMGVRHDHVVMCDRKGVIWQGREGLDQWKSAHAVITDARTLEEALVGADVFLGL
                     SAAGALKPEMVMKMAKQPIIFAMANPDPEITPPEAKAARPDAIVATGRSDYPNQVNNV
                     LGFPFIFRGALDVRATTINDEMKIAAAEALAELARQQVPEEVAAAYGGRAHSFGADYI
                     IPAPFDPRLMEIVPAAVARAAMDSGVARLRIDDLDEYRTRLRSRLNPTTSVLSLAYEA
                     ARAEPKRVLFAEGDEDVVLRAAIAFRDGGYGVPVLVGRDDVHDRLRALGVDDPESFEL
                     LNSRVYGRVPEMVDYLYKRMQRRGYLQREIERMVNQDRNTFAALLLALGEGDALITGV
                     TRPYAQSLRQVQRVLDPKEGMTPFGVHVLVGQAHTTFIADTTVTERPSGEQLADIAVH
                     TAQVARRMGHEPRVAFLSYSNFGDPEGTYLERIREGVKVLDRREGVDFEYEGEMSPDV
                     ALNPAMARVYPFSRLTGPANVLVMPGLQTANISAKLLRELGGDAVIGPILVGMAQPVQ
                     IAAMTASASDLVTLGVLAGSGVVR"
BASE COUNT          736 a         1676 c         1697 g          758 t
ORIGIN      
        1 tggcgcccga ccggggcagc gccgcctcga gcttcctcgg ggccttcacg atcctgcttc
       61 gcgaaggcct ggaggcgctt ctgatcgtcg tcgccatgct cgccttcctg cgcaaggccg
      121 accgggccga gatggtccgg ccggtccact ggggctggat cgccgctctg atcgccggag
      181 tcgcgacctg gtgggccgcg acccacctcg tctcgatcag cggggcgagc cgcgagctga
      241 ccgagggctt cggctcgatc ctcgccgcgg tgatcctgct gttcgtcggc atctggatgc
      301 acggcaaggc gcaggccgac gaatggcagc gctacattcg cgagaaggtc ggccaggccc
      361 tgtccagaaa atcggcctgg ttcctgttcc tgctcgcctt catcgccgtc tatcgcgagg
      421 tgttcgagac gatcctcttc ttcgccgcct tgtccgccga agggcgcctg gcgccgctgc
      481 tcggcggggc cgcggcggcg gcggcgcttc tcggcctgat cgcggtcctg atgttgcgct
      541 acagccgcaa gctgccgatc gccaaattct tcacctacag ctcggcgctg atcgccatgc
      601 tggccgtggt cctggccggc aagggggtgg cggctctgca ggaggccggg atgatcggcg
      661 tgacgccgct cggctccgcg ccgcgggtcg agatactcgg cctttacccc accgtcgaag
      721 ggctgctcgc acagctcgcc gctctggccg tcctgctggc gggcttcggg cgcaaccggc
      781 gaggggcaaa ccggctggcc accacctaaa ctagtggcgg cgcgcgggcg gctcgcttat
      841 cgtccgcgcg aatccgaaaa gcattggaag gaagagccat ggcgggcgtc aacaaggtca
      901 tattggtcgg caatctcggc gacgaccccg agagccgctc gctcaacaat gggggcgagg
      961 tggtcaacct gcgcgtggcc acgtcggaga attggaagga caaggacggc aaccgccagg
     1021 agcggaccga gtggcaccgc gtcgtgatct tcaacgagaa tctgggccgg gtcgccaaat
     1081 cctatctgcg caagggctcg aaagtgtatc tcgaaggcca gctccagacg cggaaatgga
     1141 ccgaccagtc gggcgccgac cgctactcga ccgaagtggt gctgcagcgc ttccgcggcg
     1201 agctcgtcct tctcgacagc cgcggcggcg gcggtggcgg cggcggggag gatttcggcg
     1261 gcggctacga gagcggcggc agctccggat tcggcggcgg cggatcgcgg ccccagtcgc
     1321 gccccgcgcc ggcgttcgac agcgacctgg acgacgacgt cccgttctga cggcacgcgt
     1381 cattcaggac aggtcgaggc ggcgttactt ctttagcgcc ttgaggaccg cgaacgggct
     1441 cgacgcggcg cgcgcctccg cctcgtcctg gactcccgct tcgcgcaatg ccgcctcggc
     1501 ggcgggcgag cgcggatagg ggtcgaggct cagcgccaga gtctgcgcga cggcttcgcc
     1561 gagatcgacc tggctcccgt cgtagaaaat gacgtcgagc tcgtcctcga cgagctcgac
     1621 ctcctcctcg gcgtcgccgc ccgccggcaa gggccggaac tccactcgga attcttcttc
     1681 gaccgcggcc tcgaccggct cagccgtggc gatgcagctc tgggtcacgc gcgcccgcag
     1741 aaagccggcg gcgatgaccg tctcgccgtg gcggccgagc tctgcctcgg cagccagcgc
     1801 atctacggaa acgagctcga atcgggcggc gagcgcgtcg cgttcgtccg gctcggcctc
     1861 gatgcggacg cggcgcggct cccgggcgag ggcgtcgagg cgaaaaaggc ggctgaactc
     1921 ggtcatgcgt cgggaagcga tccggcgagc aacggttcca ttccggttgc gccaagagcg
     1981 cggtggaagc ggccgagccg ttcggcgacc caggcgacct gagcgtcgcc ggcaggcgcg
     2041 tcgcgaaaga cgttgcgccg caccgccgct tcgagcgggg cgccgtcgcc caaggcctcg
     2101 cgcagcaccc cgagccgtcc gcccaacgct ccgaccagct tgccgacatg cttgccgacg
     2161 acgaagtcgc cgatcccgat ctgccgcagc gtgccgtcca tgtcgtcgat gaaaatctct
     2221 gtgagctgca ccgactcggc ccttgcacgg tcgccttccg cctccagcct gaggaggacg
     2281 agcgcgacca tcgtcgcgac catgtcgaaa cggccctcga gcgtgtccgg gactccgccc
     2341 tcgcggtacc aggccgggtc gcgggccgcg gcgaccagcg cgtcgtagag cggccgcagc
     2401 gccgcgcgct cgcggcgcgc gccgaacagt cgggacagaa aggccagggc gggtctcctc
     2461 gagcggggcg catacttgcg ccgttcagct tttctcggat attggagggc ttggccctgc
     2521 cggcaaggca ggcgccggcg ctcgaatcct gcgccggccg gttttcggga gtttttccaa
     2581 tatgatggtt tcgggacgaa aagcggcagt gctggcggcc gttgccgcgg ggctcgcgct
     2641 cggcggctgc gccaagacgc gcgacatcca gggctatctc gtggacgaga ctctggtggc
     2701 cgccgtccag cccggcatcg acaacaagga atcggtgcag aacacgctgg gccggcccac
     2761 gttcacgggc acgttcgacc agaacgactg gtattacgtg tcccgccgga ccaagaacta
     2821 cgcctacagc caccccaagg tgaacgagca gacggtgctc cacgtccgct tcgacgcggc
     2881 cggcaacgtg gccgcggtcc agcgcaaggg cctcgagcaa gtcgtgtcga tcgacccgat
     2941 gcacgacaag acgccgaccc tgggccgcaa caagagcatc tgggacgaat tgttcggcaa
     3001 tatcggtgcc gtcggcgcgc cggtcggctc cccgggcggc cccaactccc cgaactaggc
     3061 cggaaatccc gcgcgatctc gcggagagga acgcctagcg gacgacgccg ctgcccgcga
     3121 gcacgcccag ggttaccagg tccgacgcgc tcgccgtcat cgccgcgatc tggaccggct
     3181 gcgccattcc gaccaggatc gggccgatca ccgcgtcgcc gcccagctcc cgaagcagct
     3241 tggccgagat gttcgcggtc tggaggcccg gcatgacgag gacgttggcc ggaccggtca
     3301 ggcggctgaa cggatagacc cgcgccatcg ccgggttgag cgcgacgtcg ggcgacatct
     3361 cgccctcata ttcgaagtcg accccctcgc gtcggtcgag gaccttcacg ccctcgcgga
     3421 tccgctcgag ataggtgccc tcgggatcgc cgaagttcga ataggacagg aaggcgacgc
     3481 gcggctcgtg acccatccgg cgggcgacct gcgcggtgtg gacggcgatg tcggcgagct
     3541 gctcgccgct cggccgctcg gtgaccgtgg tgtcggcgat gaacgtcgtg tgcgcctgcc
     3601 cgacgagcac gtggactccg aacggagtca tgccttcctt cgggtcgagc accctttgca
     3661 cctgccgcag gctctgcgca tagggccggg tgaccccggt gatcagagcg tcgccctcgc
     3721 ccagcgcgag gagcagggcc gcgaaggtgt tgcggtcctg gttcaccatc cgctcgatct
     3781 cgcgctgcag atagccgcgc cgctgcatcc tcttgtagag atagtcgacc atctccggca
     3841 cgcggccgta gacccggctg ttgagcagct cgaagctttc cggatcgtcg acgcccaggg
     3901 cacgcagccg gtcgtggaca tcgtcgcggc cgaccagcac cggaacgccg tagccgccgt
     3961 cgcggaaggc gatggcggcc cgcaggacca cgtcctcgtc gccttccgcg aacagcaccc
     4021 gtttgggctc ggcgcgcgcg gcctcgtagg cgaggctgag caccgaggtg gtgggattga
     4081 gccgggaccg aagccgcgtc cggtactcgt ccagatcgtc gatgcgcagc cgcgcgactc
     4141 cgctgtccat cgcggcccgg gccacggcgg cggggacgat ctccatcagc cgcggatcga
     4201 aaggcgccgg gatgatatag tccgctccga agctgtgcgc ccggccgcca taggcggcgg
     4261 cgacctcctc tgggacctgc tggcgggcga gctccgcgag cgcctcggcg gcggcgatct
     4321 tcatctcatc gttgatcgtc gtcgcccgga cgtcgagggc gccccggaag atgaagggga
     4381 agccgaggac gttgttgacc tggttgggat agtcggagcg gccggtcgcg acgatcgcgt
     4441 cgggccgagc cgccttggcc tccggcgggg tgatctccgg atcgggattg gccatggcga
     4501 agatgatcgg ctgcttcgcc atcttcatca ccatttcggg cttgagcgcc cccgccgccg
     4561 agaggccgag gaagacgtcg gcgccgacca gcgcctcctc cagggtgcgg gcgtcggtga
     4621 tcaccgcgtg ggccgacttc cactggtcga gcccctcccg cccctgccag atgacgccct
     4681 tgcggtcgca catgacgaca tggtcgtgcc tgacgcccat tgccttgatc agctcggtgc
     4741 aggcgatcgc cgccgcgccc gcgccgttga cgaccacctg gatgtcggtc agcgcccggc
     4801 cggtcaggta gcaggcgttg atcaggccgg cggcggtgat gatcgcggtg ccgtgctggt
     4861 cgtcgtg
//
LOCUS       DASVQZ010000227         6604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_817585, whole genome shotgun sequence.
ACCESSION   DASVQZ010000227 DASVQZ010000000
VERSION     DASVQZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817585"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VF547_06165"
     CDS             <1..562
                     /locus_tag="VF547_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8262440.1"
                     /translation="ERIHVIAPHPLATMRFHDMRLSPNALLGERGRGFAQAMATLDVF
                     RTTVGAAALGFARRALDEATKRALTRRLGSGTLADNPITQSKLAEMVLDVDSSALLIY
                     RAAWLRDSTGRRNSREAALAKLHATDSAQSVIDKAVQLFGGLGVTRGVPVERLYREIR
                     ALRIYEGASEVQKVVIARAHLAEYRE"
     gene            559..954
                     /locus_tag="VF547_06170"
     CDS             559..954
                     /locus_tag="VF547_06170"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8262441.1"
                     /translation="MRTLLPPGWPRPSGFSNGVSAEGRMIFTAGIIGWDERGTIVAPD
                     LAGQLRQILLNTLAILAEDGAGPEHVVRMTWYVVDMNEYRFGLAEIGAAWKEMMGRNF
                     PAMAVVQVESLVEPAARIEIETTAVVPRW"
     gene            1064..2653
                     /locus_tag="VF547_06175"
     CDS             1064..2653
                     /locus_tag="VF547_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8262442.1"
                     /translation="MTAHLDTFVRDRLPPAEAQPEFLFDLPELQYPERLNAAVELIDR
                     GDPAALAVINDCGRWTYGEMSALSDRIARLLVEEEDLLPGDRVLLRGPNGAMLFACWL
                     GVLKAGGIVVATMPVLRAGEVATIVERARIHHAIVDDRCLGDFMAAADETGLIRSLLT
                     YGGDSGEGRLERRLEAIGPGFVPAATRRDDVALIAFTSGTTGRPKGCVHYHRDVLACA
                     DSFARHVLKPRPGGRWACSAPIAFTFGLGMLLIFPWRFGGAAVTIEQPGPKPLLEAVA
                     KFEITHLATAPTAYKAMLGLLGEHDISSLRTCVSAGEHLPAATWQAWHDATGLRIVDG
                     IGATEMMHIFISASGDDIRPGATGKAVPGYRACVLDEAGRPLPHGIGRLAVKGPTGCR
                     YFDDERQSDYVQGGWNVTGDTYRADEDGYFWYVARSDDMIISAGYNIAAPEVENALYA
                     HPAVQECAVVGVPCEERGRKVKAFVVPAPGRAPDEALARELQDHVKATIAPYKYPREV
                     EFVAALPKTATGKLQRFALRR"
     gene            complement(2792..4405)
                     /locus_tag="VF547_06180"
     CDS             complement(2792..4405)
                     /locus_tag="VF547_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262443.1"
                     /translation="MSRRPYPVDVEYADIQGGILTAYGKQGFPWGRFGLVCVRNPAAG
                     RAFVEKLRHEVTTAVRWPSSKQAIPEGRLMVERPAVTLNLAFTYCGLKALGVPTRTLR
                     GLPDEFMDSMAGRAKILGDDTPNNPMAEWDEVWNPKGRGADVHILVMLNGTITPDGNP
                     PPQLQAMQDRIASYCDGRDVELLAGHRGPDTHWQRLEAIKDDKGMPCPKEHFGFTDGI
                     SDPVFEGQYPKQYEKQRAVGSGATDGEGNWRPLAAGEFLLGWPDEAQEIPGAAMPLDF
                     SRNGTFMAYRKLNQEVAAFKAWVNEAAAQLQQVWNLPSFEVAHATLMAKMAGRWEDGV
                     PLEVAPDWEAWQKFNAECQPDKEGHVVRDESARAKALVDFGYRTDADGYRCPMGAHIR
                     RCNTRDMLDPRGDSPNPKSRMGSALNNRRRILRRGLPYGKENDADGECGIIMLIVCAS
                     LQRQFEFIQQQWLNYGLDANSGNDTCPLLGNHDEESKFVIPADPDSGTPPFIATRLKQ
                     FVTTRGGDYFFVPSMTALRMIGMGVVDPT"
     gene            complement(4402..5364)
                     /locus_tag="VF547_06185"
     CDS             complement(4402..5364)
                     /locus_tag="VF547_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262444.1"
                     /translation="MPTFDTGTLFYTGLFPVDLRPTRRPDKSITSHSHLLREVLASLP
                     NYSPEDRAALPSPFSRDSRTHFARFAIIDDPAFEGRVSGNTLLAAAEGVDPLVHQPVD
                     HLSRSWLIFVADADAKDAGDSCRDLWARGLWEVMEPELRAIFRHCLGFRKVESGDDFA
                     AYLAKGQVETTMSFHDYWIDPPNLPDLSMARIKGTILLPALLGALAVAWWRWWPWHWP
                     SWPLDWGGLLLVLLGALIGLAIGIWLIIRTISSRGAKPWPAGADSDLPSVLKSLHVQR
                     EFARFAIDNQAAAPAELHAAFGRFIDEVKPADVAGPTQKAGVVP"
     gene            complement(5364..6017)
                     /locus_tag="VF547_06190"
     CDS             complement(5364..6017)
                     /locus_tag="VF547_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262445.1"
                     /translation="MSNVAGKAYAMNVVTPMRPSRTKLMRLIFMFARSQPGKLAGLLG
                     LSFIHFARWVLIRGDQWPDFGQPKERLNNDYMLFCSNFNGTWDQYIDAYSDGLPSGLD
                     LLWYTSIKYPHSIPITPFKNYIRANQIDTDYYYNSVPGAAQKDVKAAMRLRRAIKALA
                     AQHGSMSPEEFAAAYRKTLLAVQNDLRGQGPAPVASNDTAAADLNRLPYVKQAWGAV"
     gene            complement(6256..>6604)
                     /locus_tag="VF547_06195"
     CDS             complement(6256..>6604)
                     /locus_tag="VF547_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262446.1"
                     /translation="AAALAAAAAAAVNPADQAQAPGTIDSFARAVLRRYEPPNAEAST
                     AAVQARSAKPLPAATHWALSGLPQPSATAPAAKPAKPAATAAAPAEPPKCLEGVRRAL
                     KGVPAPADKAAAC"
BASE COUNT         1023 a         2237 c         2296 g         1048 t
ORIGIN      
        1 cgagcgcatc catgtcatcg cgccccaccc gctcgccacc atgcgcttcc acgacatgcg
       61 cctctctccc aacgcattgc tcggcgagcg cggacggggc tttgcgcagg cgatggcgac
      121 gctcgacgtc ttcaggacca cggtcggcgc ggcggccctc ggcttcgccc gtcgtgccct
      181 cgacgaggcg acgaagcgcg ccctcacccg ccggctcggt tcgggaacgc tcgccgacaa
      241 tccgatcacc cagtcgaagc tcgccgagat ggtgctcgac gtcgatagct ccgccctcct
      301 catctaccgc gccgcctggc ttcgcgactc gacgggtcga cggaacagcc gcgaagccgc
      361 gctcgccaag ctgcacgcca ccgacagcgc ccaaagcgtg atcgacaagg ccgtgcaatt
      421 gttcggcggc ctcggagtca cccgcggcgt cccggtcgag cggctctacc gcgagatacg
      481 ggcgctgagg atctacgagg gcgcctccga ggtgcagaag gtggtgatcg cccgcgccca
      541 tctggcggag tatcgcgaat gaggaccctg ctcccccccg gctggccgcg gcccagcggc
      601 ttttcgaacg gcgtctccgc ggaaggacgg atgatcttca ccgcgggaat catcggctgg
      661 gacgagcgcg ggaccatcgt cgcgcccgat ctcgccgggc agcttcgcca gatcctgctc
      721 aacacgctcg ccatcctcgc cgaggacggc gccgggcccg agcatgtcgt gaggatgacc
      781 tggtacgtcg tcgacatgaa cgaatatcgc ttcggcctcg ccgagatcgg cgccgcctgg
      841 aaggagatga tgggccgcaa tttcccggcg atggcggtgg tccaggtgga atcgctggtc
      901 gagcccgccg cccggatcga gatcgagacg accgccgtgg tgcccagatg gtagctggag
      961 ccgtcattgc gagcgaagcg aagcaatcca gtgctgcggc tgtcgcgggc gcagcgctgg
     1021 attgccgcgt cgcttcgctc ctcgcaatga cgggcctgcc ggcatgaccg cccatctcga
     1081 caccttcgtc cgcgaccgac tcccgccggc cgaggcgcag cccgaattcc tcttcgacct
     1141 gcccgagctc caatatcccg agcgcctcaa cgcggccgtc gagctgatcg acaggggaga
     1201 tccggccgcc cttgcggtga tcaacgattg cggccgctgg acctatggcg agatgagcgc
     1261 gctctccgat cgcatcgccc gactgctggt cgaggaggag gatctccttc ccggcgaccg
     1321 ggtgcttctg cgcggcccca atggcgcgat gctgttcgcc tgctggctcg gagtcctcaa
     1381 ggcgggcgga atcgtcgtcg cgaccatgcc ggtgcttcgg gcgggggagg tggcgaccat
     1441 cgtcgagcgc gcgcgcatcc accacgccat cgtcgacgac cgatgcctcg gcgacttcat
     1501 ggcggcggcc gacgagaccg gcctgatccg ctcgctcctc acctacggcg gcgattcggg
     1561 cgagggacgg ctggagaggc ggctggaggc gatcgggccc ggcttcgtcc cggccgccac
     1621 ccgccgcgac gacgtcgccc tgatcgcctt cacctcgggc acgaccggac ggccgaaggg
     1681 ctgcgtccat tatcaccgcg acgtgctcgc ctgcgccgac agcttcgcgc gccacgtgct
     1741 gaagccgcgg ccgggcggcc gctgggcctg ctcggcgccg atcgccttca ccttcgggct
     1801 cggcatgctg ctgatcttcc cctggcgctt cggcggcgcg gcggtgacga tcgagcagcc
     1861 cgggccgaag ccgctgctcg aggccgtggc gaagttcgag atcacgcacc tcgccaccgc
     1921 cccgaccgcc tacaaggcga tgctcggcct gctcggcgag cacgacatct cgtccctgag
     1981 aacctgcgtc tcagcgggcg agcatctccc ggcggccacc tggcaggcct ggcacgatgc
     2041 gaccggcctg aggatcgtcg acggcatcgg cgcgaccgag atgatgcaca tcttcatctc
     2101 cgcctccggc gacgacatcc gcccgggggc caccggcaag gccgtcccgg gctaccgggc
     2161 ctgcgttctc gacgaggcgg gccgccccct cccccacggc atcggacggc tggcggtgaa
     2221 ggggccgacg ggctgccgct atttcgacga cgagcgccag agcgactacg tccagggcgg
     2281 ctggaacgtg accggggaca cctatcgcgc cgacgaggac ggctatttct ggtacgtcgc
     2341 ccgctcggac gacatgatca tctcggccgg ctacaacatc gccgcgcccg aggtcgagaa
     2401 cgccctctac gcccatcccg cggtgcagga atgcgccgtc gtgggagtgc cctgcgagga
     2461 gcgcggccgg aaggtgaagg ccttcgtggt gcccgcaccc ggccgagcgc cggacgaggc
     2521 gctcgcccgc gagctccagg atcacgtcaa ggcgacgatc gcgccctaca aatatccgcg
     2581 cgaggtggag ttcgtggcgg ccctgcccaa gaccgccacc ggcaagctcc agcgcttcgc
     2641 attgcggcgc taaatcccgt catgccagcg aaggctggca tccatgccga atccgcggac
     2701 cctctccgac tcggcatgga ccccagcatt cgctggggtg acggaaggga acggagattc
     2761 gacttgatcc gatcatagca ctgccgggca tctaggtcgg atccaccacc cccatgccga
     2821 tcatcctcag cgccgtcatc gacggcacga agaaatagtc gccgccgcgg gtggtgacga
     2881 actgcttcag cctcgtcgcg atgaaggggg gggtgcccga atcgggatcg gcggggatga
     2941 cgaacttcga ctcctcgtca tggttgccga gcagaggaca ggtgtcgttg cccgaattgg
     3001 cgtcgaggcc gtagttcagc cattgctgct gaatgaactc gaactggcgc tgcaggctcg
     3061 cgcagacgat cagcatgatg atcccgcact cgccgtcggc gtcgttctcc ttgccgtagg
     3121 gcaggccgcg gcggaggatc cggcggcggt tgttgagcgc cgagcccatg cgcgatttgg
     3181 gattgggcga gtcgcctcgc ggatcgagca tgtcgcgggt gttgcagcgg cggatgtggg
     3241 cgcccatcgg acatctgtat ccgtcggcat ccgtccgata gccgaagtcg accagcgcct
     3301 tggcccgcgc gctctcgtcg cggacgacat ggccctcctt gtcgggctgg cattcggcgt
     3361 tgaacttctg ccacgcttcc cagtcgggcg ccacctccag cggaacgccg tcctcccagc
     3421 ggcccgccat cttcgccatc agcgtggcat gcgccacttc gaagctggga aggttccaga
     3481 cctgctggag ctgggccgcc gcctcgttca cccaggcctt gaacgcggcc acctcctggt
     3541 tgagcttgcg ataggccatg aaggtgccgt tgcggctgaa atcgagcggc atcgccgcgc
     3601 cgggtatctc ttgcgcctcg tcgggccagc ccaggaggaa ctcgccggcg gcgagcgggc
     3661 gccaattgcc ttcgccgtcg gtcgctccgg agccgaccgc gcgctgcttc tcatattgct
     3721 tgggatactg gccttcgaag accggatcgc tgatcccgtc ggtgaagccg aaatgctcct
     3781 tcgggcaagg catgcccttg tcgtccttga tcgcctcgag acgctgccaa tgcgtgtcgg
     3841 ggccgcgatg gccggcgaga agctcgacgt ccctgccgtc gcaataggaa gcgatgcggt
     3901 cctgcatcgc ctggagctga ggcggcggat tcccgtccgg cgtgatcgtg ccgttgagca
     3961 tgacgaggat atggacgtcc gccccccgcc ccttcggatt ccagacctcg tcccattcgg
     4021 ccatgggatt gttgggcgtg tcgtcgccga ggatcttggc ccggccggcc atcgaatcca
     4081 tgaactcgtc gggaaggccg cgaagcgtcc gggtcggaac gccgagcgcc ttcaggccgc
     4141 aataggtgaa ggccaggttc aaggtgacgg cgggccgctc gaccatcagc cgtccctcgg
     4201 ggatggcctg cttgctcgaa ggccagcgca ccgcggtcgt cacctcgtgg cggagcttct
     4261 cgacgaaggc ccgccctgcg gccggattgc gcacgcagac cagcccgaag cgcccccagg
     4321 ggaagccctg ctttccatag gcggtgagga taccgccctg gatgtcggca tattcgacgt
     4381 cgacgggata aggtcgtctg ctcacggcac cactcccgct ttctgggtcg gccccgcgac
     4441 gtccgccggt ttcacctcgt cgatgaagcg cccgaaggcg gcgtggagct cggcgggcgc
     4501 cgcagcctgg ttgtcgatcg cgaaccgggc gaactcgcgc tgcacgtgga ggctcttgag
     4561 gacgctcgga aggtcggagt cggccccggc cggccagggc ttggcgccgc ggctggagat
     4621 ggtgcggatg atcagccaga tgccgatggc gaggccgatc agcgctccga gcaggacgag
     4681 caagaggccc ccccagtcca acggccagct cggccagtgc cagggccacc agcgccacca
     4741 ggcgacggcg agcgcgccga gcagcgccgg gagcaggatc gtgcccttga tccgcgccat
     4801 cgacaggtcc gggaggttgg gcggatcgat ccagtaatcg tggaagctca tcgtcgtctc
     4861 gacctggccc ttggcgaggt aggcggcgaa gtcgtcgccg gactcgacct tgcggaagcc
     4921 caggcagtgg cggaagatgg cgcggagctc cggctccatc acctcccaca ggccgcgcgc
     4981 ccacaggtcg cggcaggagt cgccggcgtc cttcgcgtcg gcatcggcca cgaagatcag
     5041 ccaggaacgg gagagatggt cgaccggctg gtgcaccaag gggtcgacgc cttcggccgc
     5101 ggcgaggagg gtattgcccg agacccgacc ctcgaacgcc ggatcgtcga tgatggcgaa
     5161 ccgggcgaaa tgggtgcggc tgtcgcggct gaacgggctc ggcagagcgg cgcgatcctc
     5221 ggggctgtaa ttcggcagcg aggcaagcac ctcgcggagc agatggctgt gcgaggtgat
     5281 gctcttgtcc gggcgccggg tcggcctgag gtcgacgggg aacaggccgg tgtagaagag
     5341 cgtgccggta tcgaaagtgg gcatcagacg gctccccacg cctgcttgac gtaaggcagc
     5401 cggttgaggt cggcggcggc cgtgtcgttg gaggcgaccg gggccgggcc ctggccgcga
     5461 agatcgttct gcacggcgag cagcgtcttc cggtaggcgg cggcgaactc ctccgggctc
     5521 atcgatccgt gctgggcggc gagcgccttg atcgcgcgcc gaagccgcat cgcggccttg
     5581 acgtccttct gcgccgcgcc cggcaccgaa ttgtagtaat agtccgtgtc gatctggttg
     5641 gcccggatat agttcttgaa cggggtgatc gggatcgagt gcggatattt gatgctggtg
     5701 taccagagca ggtcgaggcc gctcgggagc ccgtcggagt aggcgtcgat atactggtcc
     5761 caggtgccgt tgaagttgga gcagaacagc atgtagtcgt tgttcagccg ctccttgggc
     5821 tggccgaaat caggccattg gtcgccgcgg atcagcaccc agcgcgcgaa atggatgaac
     5881 gagagcccga ggaggccggc cagcttgccg ggctgcgagc gggcgaacat gaagatcagc
     5941 cgcatcagct tcgttcgcga cggccgcatc ggcgtcacga cgttcatcgc ataagctttg
     6001 ccggctacgt tcgacatggc ccccctccac gatccgcgaa gggtgaccgg aaaaggggct
     6061 caggtcaaag ccaattgtat ccgaatttgc gatgatcgag ccggattcct ccccggcatt
     6121 ggccggggag ggggaccagg cgaagcctgg tggaggggtt cctcccgcct ctggagaacc
     6181 cctccaccgc gctccgcgcg gtccccctcc ccgagaaatc tcggagcgga attcggggct
     6241 caaacccggt ccgaactagc aggccgccgc cttgtccgcc ggggcgggca cgcccttgag
     6301 agcccggcgc acgccttcca ggcacttcgg cggctcggcg ggtgcggccg ccgtcgccgc
     6361 gggcttggcc ggcttggcgg ccggtgccgt cgccgacggc tgcggcaggc cggacaatgc
     6421 ccagtgggtg gcggcgggga gcggcttggc cgagcgggcc tggacggcgg cggtcgaggc
     6481 ctccgcattg ggcggctcgt agcgccggag cacggcccgg gcgaagctgt cgatggtgcc
     6541 cggcgcctgc gcctggtcgg ccgggttgac ggcggcggcg gccgcagcgg cgagggcggc
     6601 ggcc
//
LOCUS       DASVQZ010000228         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_243845, whole genome shotgun sequence.
ACCESSION   DASVQZ010000228 DASVQZ010000000
VERSION     DASVQZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243845"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..454
                     /locus_tag="VF547_06200"
     CDS             41..454
                     /locus_tag="VF547_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8262447.1"
                     /translation="MDRDRLARVSEGQRACLRLVLEHRSSKDIARVLAISPHTVDQRI
                     RFAMKALGAGSRVEAARILAAAEAEQGYQPLIYQLPDIDRPAGPPPASAPLEPEVRNA
                     GNLPRTVWMLVAAAAALSAIAAFLIGLNALAEFTR"
     gene            475..1440
                     /locus_tag="VF547_06205"
     CDS             475..1440
                     /locus_tag="VF547_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262448.1"
                     /translation="MGSEARPARRGVRTARRLLYALAGAFCLGVAGLALLPSGQREDR
                     RWQPTLAAPHHVGSHPSVCFDEGHYNAHRATGRYRPLARLLEADGYRIERHRGRFGAA
                     SLRRCDLVVIANAAGGDRFRLGPINLPIKRGGERGDPAFSAAEIDVLRRWVEGGGSLL
                     LVADHAPFGGAARALGAAFGVEIGGGFVEVSPAGPAAAGMGAALFTAANGLLRPHPIT
                     AGLKAVKSFTGQSLAGAGTPLLVLPPDAVEYVPPGPRLKPVRAGGRAQAVALAHGRGR
                     VVVLGEAAMITAQLDDSGKPFGMNLPGIDNQAFALNLFHWLSRAP"
     gene            complement(1444..1911)
                     /locus_tag="VF547_06210"
     CDS             complement(1444..1911)
                     /locus_tag="VF547_06210"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX8262449.1"
                     /translation="MKDIGYRPAVGVMLLGPEGKVWVGQRLDSSLEAWQMPQGGLDPG
                     ESPLQGALRELEEETGIPRDLDEIVAEAPRELYYDLPPELVGKIWKEPWRGQRQTWFL
                     ARFLGSDDDVNLATPEPEFRAWKWAEPAELPAMIVPFKKKLYEEVLAAFADRL"
     gene            complement(1908..2120)
                     /locus_tag="VF547_06215"
     CDS             complement(1908..2120)
                     /locus_tag="VF547_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262450.1"
                     /translation="MNLPFVRPDVRTFLDYYNALPALPKSQGGTPPTLVQAAGLDPRR
                     AKPSSADDIGAGVDASKLILAEARTA"
     gene            2275..2514
                     /locus_tag="VF547_06220"
     CDS             2275..2514
                     /locus_tag="VF547_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262451.1"
                     /translation="MRHFKILAAVAVALMAGGVATAKEQPAKAKPKKICRVQEESATR
                     IAARRVCRVVTEDQVVRAKSEQQDARQPQAGTQTD"
BASE COUNT          374 a          989 c          957 g          406 t
ORIGIN      
        1 ccgcctttgc gcttcggccc cgcggcggta aggctgcggc atggaccggg accgcctcgc
       61 gagggtcagc gaagggcagc gagcctgcct caggctcgtg ctcgagcatc ggtcgtcgaa
      121 ggacatcgcc cgggtcctcg ccatctcgcc gcacaccgtc gaccagcgca tccgcttcgc
      181 gatgaaggcg ctcggcgccg gcagccgagt cgaagccgcg cgcatcctcg ccgccgccga
      241 ggcggagcag ggatatcagc ctttgatata tcaactgccg gacattgatc ggccggcggg
      301 tccgccgcca gcttcggcgc ctctcgagcc ggaggtgcgc aatgccggaa atctgccacg
      361 gaccgtctgg atgctggtcg ccgccgcggc ggcgctctcg gctatcgccg cgttcctcat
      421 cggcctcaac gccttggccg aattcacccg ctgaccggcg ggcggggcgg gccgatgggc
      481 tctgaggcca ggcccgcccg gcggggcgta cggacggcga ggcggctgct ctacgccctg
      541 gcgggcgctt tctgcctcgg cgtcgccgga ctggcgctgc tcccgtccgg ccagcgggag
      601 gaccggcgct ggcagcccac tctcgccgct ccccatcatg tcgggagcca cccttcggtc
      661 tgcttcgacg aaggccatta caacgcccat cgggcgacgg ggcgctaccg gccgctcgcc
      721 cgtctgctcg aggcggacgg ctatcggatc gagcgccacc gcggccgctt cggcgccgct
      781 tcgctccgcc ggtgcgacct cgtcgtcatc gccaacgccg ccggcggcga ccgtttcagg
      841 ctcgggccga tcaaccttcc gatcaagcgt ggcggcgagc gcggcgatcc cgccttctcc
      901 gcggcggaga tcgacgtcct gcggcgctgg gtcgaaggcg gcggcagcct gctcctcgtc
      961 gccgatcacg cgccgttcgg aggggcggcg agggcgctcg gagccgcatt cggcgtggag
     1021 atcggcggcg gcttcgtcga ggtgtctccc gcgggtccgg cggccgcggg catgggcgcg
     1081 gcgctgttca cggccgcgaa cggactgctt cggccccatc cgatcacggc gggcctcaag
     1141 gcggtgaaga gcttcacggg ccagagcctc gccggcgccg gcacgccgct gctcgtcctg
     1201 cccccggacg cggtcgaata tgttcccccc gggccgcggc tgaagccggt ccgggcgggg
     1261 ggacgggcgc aggccgtcgc cctagcgcat ggccgggggc gagtcgtcgt cctcggcgag
     1321 gcggcgatga tcaccgccca gctcgacgac tccggcaagc cgttcggaat gaaccttccc
     1381 ggcatcgaca accaggcctt cgccctgaac ctgttccact ggctgtcgcg cgcgccctga
     1441 ccctcacagc cggtcggcga acgcggccag cacctcctcg tagagcttct tcttgaacgg
     1501 cacgatcatc gccggcagct cggcgggctc ggcccatttc caggcccgga actcgggttc
     1561 gggcgtggcc aggttgacgt cgtcgtcgct gccgaggaag cgggcgagga accaggtctg
     1621 gcgctggccc cgccacggct ccttccagat cttgccgacc agctccggcg gcaggtcgta
     1681 ataaagctcg cgcggcgcct cggcgacgat ctcgtcgaga tcgcggggta tcccggtttc
     1741 ctcctcgagc tcgcgaagcg cgccctggag cgggctctcc ccgggatcga ggccgccctg
     1801 cggcatctgc caggcctcca ggctgctgtc gagccgctgc ccgacccaga ccttcccctc
     1861 cgggccgagc agcatcactc cgaccgcggg ccgatagccg atgtccttca tgccgtccgc
     1921 gcctccgcca ggatcagctt cgacgcatcg acgccggcgc cgatgtcgtc cgccgaggag
     1981 ggtttggccc gcctcgggtc gaggccggcg gcctggacga gggtcggcgg cgtcccgccc
     2041 tggctcttcg gcagggccgg cagggcgttg taataatcga ggaacgtgcg tacgtccggc
     2101 cgcacgaagg ggagattcat cgtcgctcca ttccatccgc ccgggggggc tggaagcgcg
     2161 ctatagatca tcgaggggca aaacggcaga gcggctcgaa cctgatgcca agtgcttatt
     2221 atgcttgact tttttgcggc cgttgggcaa gggccgcagc cacggaggga caaaatgcgt
     2281 catttcaaga ttctggcggc ggtcgccgtc gccctgatgg cgggcggcgt cgccaccgcg
     2341 aaggaacagc cggccaaggc caagcccaag aagatctgcc gggtccagga agaatcggcg
     2401 acgcgcatcg ccgcccgccg cgtctgccgg gtcgtcaccg aggaccaggt cgtccgcgcc
     2461 aagtccgagc agcaggacgc tcggcagccc caggccggga cccagacgga ctgacgcttc
     2521 ccgcggcagc ggccgagccg ccgcggactc gggagcgggc gtgcggaggc gagttgtcgc
     2581 ttcgcgccgg ccccgctagg ggttgtccgg caagccgctg aaaaggaacc cgcgttggct
     2641 acggccaccc attgcgtcac ccccgaggaa gccgcttccg acgcggcgcc cgaagccgtc
     2701 gtcgtccgct tcgccggcga cagcgg
//
LOCUS       DASVQZ010000229         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_817918, whole genome shotgun sequence.
ACCESSION   DASVQZ010000229 DASVQZ010000000
VERSION     DASVQZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817918"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..1188
                     /locus_tag="VF547_06225"
     CDS             424..1188
                     /locus_tag="VF547_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262452.1"
                     /translation="MVRRTLVLLASTSLALGACVQTRQVADVAFEPPQGDYSLIVMRP
                     DVSVGLLTAGGLVEPRADWTERARANLLKALAEQQADRGGKTRILETRGGVAGVSADT
                     VAELERLHAAVGASIALHKYLGANLPTKRRQGLDWTLGEDAVAFGKATGMDYALFLHA
                     RDSFASSGRVALQVLGIAGCFIGFCAPQAGGSQSAYASLVDLRTGEVVWFNVLQTSSL
                     APGIQFGDIRTPEGAAQMVERLLGRMKAGRHVRARR"
     gene            1185..>1778
                     /locus_tag="VF547_06230"
     CDS             1185..>1778
                     /locus_tag="VF547_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8262453.1"
                     /translation="MSHWHEALPLTRRAMLGGLGCACCAGIAGTAEARIRPADMKPLV
                     TPGHRPSDKDEQGLWQQYERVEEEIAGSNLLVRDPALAAYVGRVAERTGGPAAKDLRV
                     YLAHIPEFNAFMAPTGFMVVFSGLLLRMRDEAQLAGVVAHEAGHFLRRHQIRMWRDLK
                     RKTAALNFIGMAAGLGYGATGIYAGDLVRLVHLGGILS"
BASE COUNT          254 a          653 c          611 g          260 t
ORIGIN      
        1 cgcgccgctc gtctattcct acaccgcgct tctggtccgg cgcggcggcg agctcaagat
       61 ccggctcgag aacgaggcca ccgcctcgga gcgcggaacc cggacggcgc agcgctaggc
      121 cgctcggcag tcggatcgcg ctctcttgcg ttcgggctga gcttgtcgaa gcggtctcct
      181 tttctctgat gcaacgacaa ggaaagccct tcgacaggct cagggcgaac gggcgggagc
      241 ggtccgaccc atccctgcaa cgctctagcg gccgcaggac ctggcccttt cgcgccgtga
      301 aggcggatcg gccgacgccg gaagcgcctg tgccgaatcg ccccgaccgg aacggacccg
      361 caaccgaagc gttgccgcca agccgctagc gcggaacgat tttctcttga ccgggagccg
      421 ggcatggttc gccgcacgct ggtcctgctt gcctccacct cgctcgcgct cggcgcctgc
      481 gtccagaccc gccaggtcgc cgacgtcgcc ttcgagccgc cgcagggcga ttacagcctg
      541 atcgtgatgc gtcccgacgt cagcgtcggg ctgctcaccg ccggcggcct cgtcgagccg
      601 cgcgccgact ggaccgagag ggcgcgcgcc aatctgctca aggccctcgc cgagcagcag
      661 gccgaccgcg gcggcaagac ccggatcctc gagacgaggg gcggggtcgc gggcgtgagc
      721 gccgacaccg tcgccgagct cgagcggctc cacgccgcgg tcggagcgtc gatcgcgctc
      781 cacaaatatc tcggcgccaa tctcccgacc aaacgccgcc agggcctcga ctggaccctg
      841 ggcgaggacg ccgtcgcctt cggcaaggcg acggggatgg actatgcgct gttcctccac
      901 gcccgcgaca gcttcgcctc gtcgggccgg gtggcgcttc aggtgctcgg gatcgccggc
      961 tgcttcatcg gcttctgcgc cccgcaggcc ggcggcagcc agtcggccta tgcctcgctg
     1021 gtcgatctga ggacggggga ggtcgtctgg ttcaacgtcc tccagacctc gagcctggcg
     1081 ccggggatcc agttcggcga catccgcacc cccgagggcg ccgcccagat ggtcgagcgg
     1141 ctgctcggcc ggatgaaggc gggccgtcac gtccgcgccc gccgatgagc cactggcacg
     1201 aggcgctgcc gctgacccgc cgggcgatgc tcggcggcct cggctgcgcc tgctgcgcgg
     1261 gaatcgccgg caccgccgag gcgcggatcc ggcccgccga catgaagccg ctcgtcaccc
     1321 ccggccaccg cccgtccgac aaggacgagc agggcctgtg gcagcaatat gagagggtcg
     1381 aggaggagat cgccggctcc aacctgctcg tgcgcgaccc cgcgctcgcc gcctatgtcg
     1441 gccgggtcgc cgagcggacc ggcggaccgg cggcgaagga ccttcgggtc tatctcgccc
     1501 acatccccga gttcaacgcg ttcatggcgc cgaccggctt catggtcgtc ttctcgggcc
     1561 tgctcctgag gatgcgcgac gaggcgcagc tcgccggagt ggtcgcccat gaggccggcc
     1621 atttcctcag gcgccaccag atccgcatgt ggcgggacct caagcgcaag accgcggccc
     1681 tcaacttcat cggcatggcg gcgggcctgg gctacggggc gaccggaatc tatgcggggg
     1741 acctcgtcag gctcgtccat ctggggggca tcctctcc
//
LOCUS       DASVQZ010000230         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_817980, whole genome shotgun sequence.
ACCESSION   DASVQZ010000230 DASVQZ010000000
VERSION     DASVQZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817980"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VF547_06235"
     CDS             <1..957
                     /locus_tag="VF547_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8262454.1"
                     /translation="DRVSKRRDSSGENWISAGGRGFRLDPHSPLARESWLAVGEVSGT
                     AAGARILSAAAIAEAEVEALFGDRIETGTRLDFDPATGGVRARRGRRLGAITLSEGQD
                     ARASPQEVAAALVEGVRANGLHLLPWSEAARSLRRRAAFARLHDPGLPDLSDEALLGS
                     LDQWLPLLAAGKRSLREIDPAALSGLLDSRLGWEGRKAVDRLAPHAFETPAASSHPID
                     YGAEAGPTVTVRVQALYGLREHPTICAGRVPLVLSLTSPAGRPIQTTRDLPGFWRGSW
                     AAVAREMRGRYPKHPWPDDPAAADPTLRTKKASSRGPGRPGG"
     gene            1008..1289
                     /locus_tag="VF547_06240"
     CDS             1008..1289
                     /locus_tag="VF547_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX8262455.1"
                     /translation="MAAARIFQRSKNAMQSGKARADEWVLQFESHRPHVPDPLTGWSG
                     GGDTQAQVTIAFPTLEAAQAYADKYGIACHLVPPAERKLKLQAYADNFK"
     gene            1286..1660
                     /locus_tag="VF547_06245"
     CDS             1286..1660
                     /locus_tag="VF547_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262456.1"
                     /translation="MIALLMLVGAASASLDAETPFEAAGRSFADAAACKAFLADRAGE
                     ARGKAYDAVEGPYEVAAGDVRIHTVRAEGQGHRIDEQRCLGAALSGRSWRHSLEDDGE
                     AEEPFTAESIARSAAWLKKGGH"
     gene            complement(1841..2380)
                     /locus_tag="VF547_06250"
     CDS             complement(1841..2380)
                     /locus_tag="VF547_06250"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8262457.1"
                     /translation="MTLRQAQDERGEPEVVWRGRFIEARKLGKWEFVSRTGGVTAAVI
                     VAVDDGHILLVEQYRTPIAARCLELPAGLVGDEEQGEEIDAAAIRELEEETGYRAARM
                     VDLGRYYASPGMSSEGFTLLRAEGLEKVGEGGGVAGEDIVVHRVRLEEVAAFAEARRA
                     QGVALDSKLLLLLGSGMLG"
BASE COUNT          359 a          965 c          840 g          325 t
ORIGIN      
        1 gaccgcgttt cgaagcgccg cgattcgtcc ggcgagaact ggatcagcgc cggcggccgc
       61 ggcttccgcc tggatcccca ctcccccctg gcgcgcgagt cctggctggc cgtcggcgag
      121 gtcagcggga ccgcggccgg cgcccgcatc ctctccgccg cggcgatcgc cgaggccgag
      181 gtcgaggccc tgttcggcga ccgcatcgag accggcaccc ggctcgactt cgatccggcc
      241 accggcggcg tccgcgcccg ccgcggccgc cggctcgggg ccatcaccct gtccgaaggc
      301 caggacgcca gggcctcgcc gcaggaagtc gccgccgccc tggtcgaggg ggtgcgcgcc
      361 aacggcctcc atctccttcc ctggagcgag gccgcgcgat cgctgcgccg ccgcgccgcc
      421 ttcgcgcgcc tgcacgatcc cggcctcccc gatctgtcgg acgaggccct gctcggatcg
      481 ctcgaccaat ggctgcccct gctcgccgcc ggcaagcgca gcctgcgcga gatcgacccc
      541 gccgctctgt cgggcctgct cgattcccgg ctcggctggg aaggccgcaa ggccgtcgac
      601 cggctcgccc cccatgcctt cgagactccg gcggcgagca gccacccgat cgattacggc
      661 gcggaggccg gcccgaccgt gaccgtgcgg gttcaggcgc tgtacggcct gcgcgagcac
      721 ccgacgatct gcgccggccg cgtccccctc gtcctcagcc tcacctcgcc cgccggccgg
      781 ccgatccaga ccacccgcga ccttcccggc ttctggcggg gcagttgggc ggcggtcgcc
      841 agggagatgc ggggacgcta tccgaagcat ccctggcccg acgacccggc cgccgccgat
      901 ccgacgctcc ggaccaagaa ggcttcgagc cgcggccccg ggcgcccagg cggttgatta
      961 aacggggaaa ccggggcagc aagggtccgg cagaaggagc gaaggcgatg gcggcggcac
     1021 ggatcttcca gcggtcgaag aatgcgatgc agtcgggcaa ggcccgcgcc gacgaatggg
     1081 tgctccagtt cgaatcgcac cgcccgcacg tccccgatcc gctgaccggc tggtccggcg
     1141 gcggcgacac ccaggcccag gtgacgatcg ccttcccgac tctcgaggcg gcccaggcct
     1201 atgccgacaa atacggcatc gcctgccacc tcgtcccccc ggccgagcgc aagctgaagc
     1261 tgcaggccta tgcggacaat ttcaaatgat cgcgctgctg atgctggtcg gcgcggcgag
     1321 cgcgagcctc gacgcggaaa ccccgttcga ggcggccggg cgaagcttcg ccgacgccgc
     1381 cgcctgcaag gccttcctcg ccgacagggc cggcgaggcc cgcggcaagg cctatgacgc
     1441 cgtcgaggga ccctatgagg tggccgcggg cgacgttcgc atccacaccg tccgcgccga
     1501 gggccagggc caccgcatcg acgagcagcg ctgcctcggc gccgcgctct ccggccgctc
     1561 ctggcgccac agcctcgagg acgacggcga ggcggaggag ccgttcacgg ccgaatcgat
     1621 cgcgcggtcc gcagcctggc tgaagaaagg cggacattag ccaaattcct ccccgcgatt
     1681 tcgcggggag ggggaccgcg cgaagcgcgg tggaggggct ctttccaagt cagaaggccc
     1741 cctccaccag gctccgccga aggcggagtt tatcctgagc gacgccgcag gcggcgtcga
     1801 agggcctggt ccccctcccc tgccaatgca ggggaggaat tcagcccagc atccctgatc
     1861 ccagcaggag cagcagcttc gaatccaggg cgaccccctg cgcgcgccgc gcctcggcga
     1921 aggccgccac ctcctccagc ctgacccggt ggacgacgat gtcctcgccg gccacgccgc
     1981 cgccctcccc caccttctcc agcccctcgg cccggagcaa ggtgaagccc tccgacgaca
     2041 ttccgggcga agcgtagtag cgccccaggt cgaccatccg ggcggcgcgg tagccggtct
     2101 cctcctcgag ctcgcggatc gcggcggcgt cgatctcctc gccttgctcc tcgtcgccga
     2161 ccagccccgc gggaagctcg aggcagcgcg ccgcgatcgg cgtgcgatat tgctcgacga
     2221 gcaggatatg gccgtcgtcg accgcgacga tcaccgcggc ggtgactccg ccggtgcgcg
     2281 agacgaactc ccatttgccg agcttcctcg cctcgatgaa gcggccgcgc cagacgacct
     2341 ccggctcccc ccgttcatcc tgagcctgcc gaagggtcac agctcgatca gccggtccgg
     2401 gagctcgttg ggattgtcga gggtgggcgg caggcagcgg gcgagcacgc cgccgatctt
     2461 ctcgaccgcc cgcgccatgc cctcgccgg
//
LOCUS       DASVQZ010000231         5150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_818167, whole genome shotgun sequence.
ACCESSION   DASVQZ010000231 DASVQZ010000000
VERSION     DASVQZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818167"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VF547_06255"
     CDS             complement(<1..444)
                     /locus_tag="VF547_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8262458.1"
                     /translation="MVQMAIYAGIVVAALLILGLIITRLYRRATKEIGFVRTGFGGEK
                     VVINGGALVLPVLHETMPVNLNTVRLAVERKNVDALITLDRLRIDVKAEFYVRVRPDR
                     ESIAIAAQTLGLRTMHPENLKELIEGKFVDALRSVAAGMTMNQLHE"
     gene            complement(446..1090)
                     /locus_tag="VF547_06260"
     CDS             complement(446..1090)
                     /locus_tag="VF547_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HEX8262459.1"
                     /translation="MLETIAAPANLPFSVALLVMLMIGAVEALGLGASAAHLDMDADL
                     DGGGDLLGWLGFGRVPLLMLIVLFLAFFGLAGLAVQQFAGPLTLWIAVPAAAAAALPL
                     TGLGARGLARVMPQDETTAISLDSLVGRRGTITVGTARRGSPAQARVRDVHGQVHYVM
                     VEPYDDDHAFAEGETVRLDRRDGHIFIALAKADGLETRFEVLPTLNAPLSTRRD"
     gene            1233..1721
                     /locus_tag="VF547_06265"
     CDS             1233..1721
                     /locus_tag="VF547_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX8262460.1"
                     /translation="MATPGADVSHDFRQAMRRVASTVNVITVCVGGEPMGITATAMSS
                     LSLDPPSLLICINRSASLHSPLEDVSHFCVNVLHKSQEDIARMFADRSRQALRFAEGW
                     QVDCDRPPRLADAQAAILCRRIRHFPYGTHSIFVGEVEEVTVREDVDPLVYVDGRYGG
                     AS"
     gene            complement(1858..2964)
                     /locus_tag="VF547_06270"
     CDS             complement(1858..2964)
                     /locus_tag="VF547_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX8262461.1"
                     /translation="MSLHDPKAINIAIVGVGNCASSLVQGLAHYQNGGANDQIGLMHW
                     DLGGYRPKDIKVVAAWDIDRRKVGKDVAGAIFEKPNCTAVFCDHVPQSKVQVEMGRML
                     DGYSEHMAEWPDERTFLPSDAPQPGKADVVRRLRETETDVLLNYLPVGSQEATEFYAE
                     CALEAGVAFVNNIPVFIASDAAWAERFRKAGVPIIGDDIKAQLGATIVHRVLTDLFKK
                     RGVKLERTYQLNTGGNTDFLNMSNRKRLASKKISKTEAVQSVAEHRLEDENIHVGPAD
                     YVAWQNDNQVCFLRMEGSMFGGVPMNLELRLSVEDSPNSAGVAIDMIRCAKIAKDRGL
                     AGPVHPAAAYFCKHPPVQTTDDEAYEALERFIRG"
     gene            complement(3108..3533)
                     /locus_tag="VF547_06275"
     CDS             complement(3108..3533)
                     /locus_tag="VF547_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8262462.1"
                     /translation="MALPPFHLAFPVHDLEAARAFYGGLLGCREGRSASQWIDFDFFG
                     HQIVAHLDPDMVPRRAHNPVDGHDVPVPHFGAVLDMAAWQDMAARLEAAGTSFVIEPT
                     VRFRGAPGEQATMFFLDPSGNALEIKAMADPANLFAKEP"
     gene            3632..>5150
                     /gene="lnt"
                     /locus_tag="VF547_06280"
     CDS             3632..>5150
                     /gene="lnt"
                     /locus_tag="VF547_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX8262463.1"
                     /translation="MKHVALAFLAGLAAACGFEPLGLWPLTLAAAALLLWLVRGAPRL
                     SGALARGWWFGVGHFVVGLNWIATAFTYQAEMPASLGYVAVVLLSLYLAVYPAAAAGL
                     AWRWGRGNDLRLVLLFAAAWIVTEWLRATLFTGFAWNPLGVAVLATPASYASRLIGTY
                     GLSGLTALLGGLLLLLALRRWRAAATAAAPVFVLATAGFLVPAGPVRAPGPQLRIVQP
                     NIGQQDKWREGFEDENFARLAGYSEGGSKAPRLLLWPEAAITRPLENGLRDRGHQAEL
                     RGLRRAVAGMLGEQDLLLTGGVTWRSADGFDVTSATNSVFAMDRSGRILARYDKAHLV
                     PYGEYLPMRPILSALGLSRLAPGDIDFDPGPGPRSFELPIVGKVGFQLCYEIIFSGEV
                     VDRADRPNFLFNPSNDAWFGAWGPPQHLAQARLRALEEGLPVLRATPTGISAVIDADG
                     RLVSSLKWREAGIIDARLPAARSATPFGRLGNLLPGLLALALVAAALASERIAERR"
BASE COUNT          730 a         1759 c         1774 g          887 t
ORIGIN      
        1 ctcgtgaagc tggttcatcg tcatccccgc ggcgacggag cggagcgcgt cgacgaactt
       61 gccctcgatc agctccttca gattctccgg gtgcatggtg cgaaggccca gcgtctgggc
      121 ggcgatggcg atcgattcac ggtcggggcg gacccggacg tagaattccg ccttcacgtc
      181 gatccgaagc cggtcgagcg tgatcagggc gtcgacattc ttgcgctcga ccgcgaggcg
      241 gacggtgttc agattgaccg gcatcgtctc gtggagcacc gggaggacga gggcgccgcc
      301 gttgatcacg accttctcgc cgccgaagcc ggtccggacg aacccgatct ccttggtggc
      361 ccggcggtag aggcgcgtga tgatcagccc caaaatgagc agggcggcaa cgacgatccc
      421 cgcgtagatg gccatctgga ccatgtcagt ctctcctggt tgaaagcggt gcgttcagcg
      481 tcggcagcac ctcgaaacgg gtttcgaggc cgtcggcctt ggcgagcgcg atgaagatat
      541 ggccgtcgcg gcggtcgaga cgcaccgtct ccccctcggc gaaggcgtga tcgtcgtcat
      601 agggttcgac catgacgtaa tggacctggc cgtggacgtc ccggacccga gcctgggcgg
      661 gcgagccgcg ccgggcggtt ccgaccgtga tcgtgcccct gcggccgacg aggctgtcga
      721 ggctgatcgc ggtcgtctcg tcctggggca tgacgcgggc gaggccgcgg gcgccgaggc
      781 cggtcagcgg cagagccgcc gccgccgccg cgggcacggc gatccacagg gtgagcggac
      841 cggcgaattg ctggacggcg agtcccgcca ggccgaagaa ggcgaggaac aggacgatca
      901 gcatcagcag cggcacgcgg ccgaagccga gccagccgag caggtcgccg ccgccgtcga
      961 ggtcggcatc catgtcgaga tgcgccgccg acgcgccgag gccgagcgcc tccaccgcgc
     1021 cgatcatcag catgaccagc agcgccaccg aaaatggcag gttcgccggc gcggcgatcg
     1081 tttcgagcat cttcccccgc tcccccgagt cgcatggata atcaggcttt gggtaatttc
     1141 ctagcaaaat ccatggtttg ccgcagccgc ggcggcttcc tccgaggcta agccattgcc
     1201 agcgcggcgc ttcccggcca tgggcgggag cgatggcgac cccgggtgcc gacgtttccc
     1261 acgatttcag gcaggcgatg cgccgcgtcg cctccacggt caacgtcatc accgtgtgcg
     1321 tcggcggcga gccgatgggg atcaccgcca ccgccatgtc gtccctatcg ctcgacccgc
     1381 cgagcctgct catctgcatc aaccggagcg cgtcgctgca cagcccgctc gaggacgtct
     1441 cgcatttctg cgtcaacgtt ctccacaaga gccaggagga catcgcccgg atgttcgccg
     1501 accggagccg ccaggccctg cgcttcgccg agggctggca ggtcgactgc gatcggccgc
     1561 cccgcctcgc cgacgcccag gcggcgatat tgtgccgccg aatccggcat ttcccctacg
     1621 ggacccattc catcttcgtc ggcgaggtgg aggaggtgac ggtgcgggag gacgtcgatc
     1681 cgctggtcta tgtggacggg aggtatgggg gggcgtcttg aggaggtcct tcgatacggg
     1741 ccttcgcctt cgctcagtcc ctactcagga tgagcggttg gagaattccc acgaccgctc
     1801 atctgagtag ccggtgagct tgtcgaaccg gcgtatcgaa ggacgttccg aaggatttca
     1861 ccccctgatg aaccgctcca gcgcctcata ggcctcgtcg tcggtggtct gcaccggcgg
     1921 gtgcttgcag aaataggcgg cggccgggtg gacgggcccc gccaggccgc ggtccttggc
     1981 gatcttggcg cagcggatca tgtcgatggc gaccccggcg ctgttcgggc tgtcctcgac
     2041 cgacaggcga agctcgaggt tcatcggcac gccgccgaac atcgagcctt ccatcctcag
     2101 gaagcagacc tggttgtcgt tctgccaggc cacgtaatcg gccggcccga catggatgtt
     2161 ctcgtcctcc agccggtgct cggcgaccga ctggaccgcc tcggtcttcg agatcttctt
     2221 ggaggcgagc cgcttcctgt tcgacatgtt gaggaagtcg gtgttgccgc cggtgttgag
     2281 ctggtaggtc cgctcgagct tgaccccgcg cttcttgaac aggtcggtca gcacccggtg
     2341 gacgatggtc gcgcccagct gcgccttgat gtcgtcgccg atgatcggga cgcccgcctt
     2401 gcggaaccgc tcggcccagg cggcgtcgga ggcgatgaac accgggatgt tgttgacgaa
     2461 ggccactccg gcctcgagcg cgcattcggc gtagaattcg gtcgcctcct ggctcccaac
     2521 cggcaggtag ttgaggagga cgtcggtttc ggtctccctg agccgccgga cgacgtcggc
     2581 cttgccgggc tgcggggcgt ccgagggcag gaaggtgcgc tcgtcgggcc attccgccat
     2641 atgctcggaa tagccgtcga gcatccgccc catctcgacc tgcaccttgc tctgcggcac
     2701 gtggtcgcag aacacggcgg tgcaattggg tttctcgaag atcgccccgg cgacgtcctt
     2761 gccgaccttg cggcggtcga tgtcccaggc ggcgacgacc ttgatgtcct tcgggcggta
     2821 gccgcccaga tcccagtgca tcaggccgat ctggtcgttg gcgccgccat tctggtagtg
     2881 ggcgaggccc tgcacgagcg agctcgcgca attgccgacg ccgacgatgg cgatgttgat
     2941 cgccttgggg tcgtggaggc tcatcaggga gcgggctcca gccggtcacg gatcagacag
     3001 aaggtcactt tcacctcgaa ggtcatgctg cggcctccaa acgcgtggag ccgctgaatc
     3061 ggcacatctt tgcgggctgt gcctcgccgg agcgcggagt gcaagcttca aggctctttg
     3121 gcgaacaggt tggccgggtc ggccatggcc ttgatttcga gagcgttgcc ggagggatcg
     3181 aggaagaaca tcgtcgcctg ctcgccgggc gcgccgcgga agcgcaccgt cggctcgatg
     3241 acgaagctgg tgcccgccgc ctccagccgg gccgccatgt cctgccacgc cgccatgtcc
     3301 agaaccgccc cgaaatgcgg caccggcacg tcgtggccgt cgaccgggtt gtgggcgcgc
     3361 cggggaacca tgtccggatc gagatgggcg acgatctgat ggccgaagaa gtcgaagtcg
     3421 atccactggg atgcgcttcg gccctcccgg cagccgagca gcccgccgta gaaagcgcgg
     3481 gcggcttcga ggtcgtggac cggaaaggcg aggtggaagg ggggtagggc catgccgcct
     3541 tccttgtcat cgccggcgcg gggaagcaat ccggccgatg gcgccggttt tccggattgc
     3601 ttcggcgctt cggtccccgc aatgacggcg cgtgaagcac gtcgccctcg ccttcctcgc
     3661 cggcctggcc gcggcctgcg gcttcgagcc gctgggcctg tggccgctga ctctcgccgc
     3721 ggcggcgctc ctgctctggc tggtccgcgg cgcgccgcgc ctgagcggcg ccctcgcccg
     3781 cggctggtgg ttcggggtcg gccatttcgt cgtcggcctc aactggatcg ccaccgcctt
     3841 cacctaccag gccgagatgc ccgcctcgct cggctatgtc gccgtcgtcc tgctctcgct
     3901 ctatctcgcc gtctatccgg ccgcggccgc gggcctggcc tggcgctggg ggcggggcaa
     3961 cgaccttcgg ctcgtcctcc tcttcgccgc ggcctggatc gtcaccgaat ggcttcgagc
     4021 gacgctgttc acgggcttcg cctggaatcc tctcggagtc gcagtgctgg cgactccggc
     4081 ttcctacgcc tcgcggctga tcggcaccta cggcctgtcg gggctgacgg cgctgctcgg
     4141 cggactgctc ctgctcctcg ccctgcgccg atggcgcgcg gccgcgaccg ccgccgctcc
     4201 cgtcttcgtc ctggcgacag ccggctttct cgtccccgcc ggaccggtcc gagcgccggg
     4261 cccgcagctg cgcatcgtgc agccgaacat cggccagcag gacaagtggc gcgagggctt
     4321 cgaggacgag aatttcgccc ggctcgcggg ctattccgag ggcggctcga aggcgccgcg
     4381 gctgctgctg tggccggagg cggcgatcac gcggccgctc gagaacgggc tgcgcgatcg
     4441 gggacatcag gcggagctca gggggcttcg gcgcgcggtg gcgggaatgc tcggcgagca
     4501 ggacctgctt ctgaccggcg gggtcacctg gcgctcggcc gacggtttcg acgtgaccag
     4561 cgccaccaac agcgtcttcg ccatggaccg cagcggacgg atcctggccc gctacgacaa
     4621 ggcccatctc gttccctatg gcgagtatct gccgatgcgg ccgatccttt cggctctcgg
     4681 cctgtcgcgc ctcgcccccg gcgacatcga cttcgatccg ggccccggcc cgcgcagctt
     4741 cgagcttccc atcgtcggca aggtcggctt ccagctttgc tacgagatca tcttctccgg
     4801 cgaggtcgtc gaccgcgccg accggccgaa cttcctgttc aacccctcga acgacgcctg
     4861 gttcggcgcg tgggggccgc cgcagcatct cgcccaggcc cgcctgcggg cgctcgagga
     4921 ggggctgccg gtgcttcggg cgaccccgac cggcatttcc gcggtgatcg acgccgacgg
     4981 gcggctggtt tccagcctca agtggcgcga agccggaatc atcgatgccc gcctgcccgc
     5041 ggccaggtcc gccactccct tcggccggct cggcaacctc ctccccgggc tgctcgccct
     5101 ggcgctggtc gcggccgccc tggcttcgga gcggattgcg gagcggcgca
//
LOCUS       DASVQZ010000232         6924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_149661, whole genome shotgun sequence.
ACCESSION   DASVQZ010000232 DASVQZ010000000
VERSION     DASVQZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149661"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..578
                     /gene="nusG"
                     /locus_tag="VF547_06285"
     CDS             39..578
                     /gene="nusG"
                     /locus_tag="VF547_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX8262464.1"
                     /translation="MSRWYIIHAYSGFENKVRDAIQAEAARLGLESLVEQVEVPTEKV
                     TEVRRGKKVTSDRKFFPGYVLAKLAMNDDVYHLVKNTPKVTGFLGSSGKPQPITDAEA
                     ARILNTKDEAAAAAPKTKINVDYEIGDQVKVLDGPFASFNGVVEELDFDRGRVKVGVS
                     IFGRATPVELEFEQVERVK"
     gene            922..1812
                     /locus_tag="VF547_06290"
     CDS             922..1812
                     /locus_tag="VF547_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262465.1"
                     /translation="MLTGHRLGHISCKALVRRCERMCELFNAERLQDRSRLQAAFVGA
                     LNELWNVDHFERGERLSDFLDRRAVTIQSDGGGEGGTWWRKSRLRAGAKVIASEFARG
                     GCDGWVGVEQKPIWLTPYGGEIERLCAEAKQGGLPQRERNRLAGRIRDKLGLYAIQPR
                     EGLLAFITREPLRKLLYAHDGKTDCLPVGPTAVEARGSLRFRPWPRPRGGDAFGRTYD
                     LDPEQRQLAVPPRCHGAEEAVRPRMRIAEFAECIYIGDITASATDRDEQAFLSEIGAA
                     TSDSPTLLRQMAVAVDSCPS"
     gene            1800..2702
                     /locus_tag="VF547_06295"
     CDS             1800..2702
                     /locus_tag="VF547_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262466.1"
                     /translation="MPELTRTALANGNSARWRDWLRNGIVSWCRYPGPADRARAFLPL
                     VIDPFSHYPAAELSRQIDDLLKGKAAHVGRAAADVLKSWSVTQDTGAGAAMAIEIATR
                     FNAPRLGQLCRLIVEKTPPDSEEEADRLAWAAIAALEVRGTRSDVESLGHAISRNYLW
                     NSSLMAEYSVLLGRANLRALPVRLVDVAPIVIAAPYDGRWTTAIAERLLIEFSRDDLL
                     EAFCREPGDDPETYRFRQRLYDLVVPAQPRFGESVLKGRQKHVLKLIETGHSGGGEEA
                     EGIDERFEEPPGEDEEFRMGGSRP"
     gene            2726..5200
                     /locus_tag="VF547_06300"
     CDS             2726..5200
                     /locus_tag="VF547_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262467.1"
                     /translation="MRKLGVLVEMTQIMDRNVDDGTFGKDDSEIIYVFDTNVVQMFLQ
                     PFNNPQYAELFHSSCWGSDYEAYDLINMQSCLIMGEYLLAGRLPRQNQDGRWYMMRGH
                     YAELKRQMGWVEFQIEQNIDRLKNDSEFVYQISEYQDLLDKVLKTETSDRPSIISIAK
                     DQGERAGDLERLHQLAQEDFEARASAIKSRELCRLLACDQIFEPADQLRRLRSREILG
                     KVRSFEHAFRPDRDAREAIERDMEDWDAELLEEINAKPKQYARTVKARKNDSRTLAHL
                     QWAARQRVQINHRIVFVTGDRAIYDVYRKRAAEGAEGSPFLLRPLVQYAPLFNPSSAD
                     SWVAEREKAFALVRGALEAAMVALSLNLFGKGEPKALTRARDHFVLASQKPLKNLKWL
                     SGFPALSEERLLNEQAPKLTALAAPLQRVERLMLGAYPTLLARRLESRERREAFLKSV
                     ASTHGAALAEALQSSLEEASRTAFMYSLPFMPEEIVKLLDRIKRGDGRLPRLLIGVRL
                     TFPAEQPDRPDEQLTRKDIIRRLTKADCQLDTLVERLAEVPARVFALAALLAFGVEKW
                     EAAARYADLSATASLYDRPKDGPTGESGDHFELLYMKAVGLRFTLFSREPNPAHHYYD
                     RWAQWLLEAEDALESCIVQHRGENELSRELRAISERAAIRLAYCEWYAFGRLSETRMA
                     EEAREVALRALGQAVEDLLACYHKRDAAAARALDDGHSGRHLSADTLHWATLQFHINT
                     AAAILVANELALKWPELADRVEKSIEPLPRPDDWPRTEDLPPMSRAYILAATSRWREL
                     RAIRTRGLSLPLDRVVIRGLQARVRR"
     gene            complement(5361..5603)
                     /locus_tag="VF547_06305"
     CDS             complement(5361..5603)
                     /locus_tag="VF547_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262468.1"
                     /translation="MEQAYWLARKRASAANAKQAGSSEARLAHLDLAGRYSVKAAAAA
                     SAALAREPERAQLALPVPEGRGPADPSRARRPGEGG"
     gene            complement(5761..6183)
                     /locus_tag="VF547_06310"
     CDS             complement(5761..6183)
                     /locus_tag="VF547_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262469.1"
                     /translation="MKSTAFALALLISGTAFAQIDVTAEQTAYEGDLVATTAFETEGG
                     SSGAYSAPTLAEAGPVTAGAVVEPGNSAPERDARGIPVISAAAVVPAGWNGTAAGAAM
                     GGPELDPATGEPAAPRSYPACTASVTDHCLQTYERGRR"
     gene            complement(6346..>6924)
                     /locus_tag="VF547_06315"
     CDS             complement(6346..>6924)
                     /locus_tag="VF547_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262470.1"
                     /translation="NVRLSAAAAGASARAFYRRRRRDPAFAREFRLALKMGWERLKME
                     SLRAASPFSHARDRWRNCPEAPMPKLSWDQAFQLLCLHEKSVNQGWEQPHRRRRRGES
                     DEIYSLRLQAMWRAEKQVEAEAAALRRAERFEETGDWRHADEPPPPPLPPLETVTGWS
                     RASGRPAHNPGPALFGGWRIADMEARRAGRSG"
BASE COUNT         1097 a         2363 c         2419 g         1045 t
ORIGIN      
        1 tagccccaac gtcccgcacg cgaaggtaag tcccacccat gtcccgctgg tacatcatcc
       61 acgcttattc gggcttcgag aacaaggtcc gcgacgccat ccaggccgag gccgcccggc
      121 tcggcctcga gagcctggtc gagcaggtcg aggtcccgac cgagaaggtc accgaggtcc
      181 gccgcggcaa gaaggtcacc agcgaccgca agttcttccc gggctacgtg ctcgccaagc
      241 tggcgatgaa cgacgacgtc taccacctcg tcaagaacac gcccaaggtc accggcttcc
      301 tcggctcctc gggcaagccg cagccgatca ccgacgccga ggccgcccgg atcctcaaca
      361 ccaaggacga ggccgccgcc gccgcgccca agaccaagat caacgtcgac tatgagatcg
      421 gcgaccaggt caaggtgctc gacgggccgt tcgcgagctt caacggcgtc gtcgaggagc
      481 tcgatttcga ccgcggccgg gtcaaggtcg gggtgtcgat cttcggccgg gccacgccgg
      541 tggagctcga gttcgagcag gtcgagcggg tgaaatgacc gggtcccgcg gcgcgcggga
      601 cgatcgcaag cttcgcggcc gggcggaaga atgacccgcg ggcccagtgc aacaaattga
      661 cgaataagca gaaatactga ggcgtccact tagagccgcg gtcgcgggtc acgcgcggcc
      721 ctgaatcgtc tccgggggcg aagtatccgt cgcggcccgg tggcggcccg ggcgtgccgg
      781 ccagtggcga tgcggcgctt gatcgcaccg gatgtccgtg caacatcttc gaagatgccc
      841 ctccgtacaa tcgccacgcg gatcgaagct tcgctggaag aagaagatac ggaatttcca
      901 cttcgagttt gcctcagcaa cttgctgacc ggccatcggc tcggccacat cagttgcaag
      961 gcgctggttc ggcgctgcga gcgcatgtgc gagcttttca acgccgagcg gctccaggac
     1021 cgctcccggc tccaggccgc cttcgtcggc gcgctcaacg aattgtggaa tgtcgatcat
     1081 ttcgaacgcg gcgagcgcct gtccgatttc ctcgaccgcc gcgcggtcac gatccaatcc
     1141 gacggcggtg gcgagggcgg tacctggtgg cgcaagtccc gcctgcgcgc cggagcgaag
     1201 gtgatcgcga gcgaattcgc gcgcggcggt tgcgacggct gggtcggagt ggagcagaag
     1261 ccgatctggc tcacccccta tggcggcgag atcgagcgcc tgtgcgccga ggcgaagcag
     1321 ggcggcttgc cgcaacggga gcggaaccgg ctcgccgggc ggatccgcga caagctcggg
     1381 ctctacgcca tccaaccccg ggagggcctg cttgcgttca tcacgcgcga gccgcttcgc
     1441 aagctgctct acgcccatga cggcaagacc gattgccttc cggtcgggcc gaccgcggtc
     1501 gaggcgcgcg gcagcctgcg ctttcggccc tggccgcgtc cgcggggcgg ggacgccttc
     1561 gggcgcacct acgacctcga cccggagcag cggcagctgg ccgtgccgcc gcggtgccat
     1621 ggagccgaag aggctgtccg gccgcggatg aggatcgcgg agttcgccga gtgcatctac
     1681 atcggcgaca tcaccgcctc ggcgaccgac cgtgacgagc aggcgttcct gtcggagatc
     1741 ggagcggcca ccagcgacag tcccaccctt ctgcggcaga tggcagtggc ggtagattcg
     1801 tgcccgagct gactcgcacc gctctggcca acggcaattc ggcgcggtgg cgcgactggc
     1861 ttcgaaacgg catcgtgtcg tggtgccgat accccggccc cgcggaccgg gcccgggcgt
     1921 ttcttcccct cgtcatcgat cccttctcgc attatcccgc cgcggagctt tcccgccaga
     1981 tagacgatct cctgaagggc aaggccgccc atgtcggccg cgccgcggcg gatgtcctca
     2041 aatcctggtc ggtgacccag gacaccggcg ccggcgcggc catggcgatc gaaatcgcga
     2101 cccgcttcaa tgcgcctcgc ctcggccagc tctgccggct gatcgtcgag aagacgcctc
     2161 cggattcgga ggaggaggcg gaccggctgg cgtgggcggc gatcgccgcg ctggaggtcc
     2221 gcgggacccg gtccgacgtc gagtcgctgg ggcatgcgat cagcaggaat tatctgtgga
     2281 attcgtcgct gatggcggaa tattcggttc tgctcggacg ggcgaacctg cgggcgctgc
     2341 cggtgcggct ggtcgacgtg gcgccgatcg tcatcgccgc gccctatgac ggccgctgga
     2401 cgacggcgat cgcggaacgg ctgctgatcg agttcagccg tgacgatctg ctcgaagcgt
     2461 tctgccgcga gccgggggac gatccggaga cctaccgctt caggcagcgg ctctatgatc
     2521 tcgtggtgcc ggcccaaccg cgtttcggcg aaagcgtcct caaaggcagg cagaagcatg
     2581 tcctgaagct gatcgagacc ggccactcag gcggcggaga ggaggcggag ggcatagacg
     2641 agcgcttcga ggagccgccg ggcgaggacg aggaattccg gatgggcggc tcccgcccgt
     2701 gaaccagacc ctccaaaccg aggtgatgcg gaaactcggc gtccttgtcg agatgaccca
     2761 gatcatggac cggaatgtcg acgacggcac attcggcaaa gacgattcgg agatcatcta
     2821 cgtcttcgac acgaacgtcg tgcagatgtt cctccagccc ttcaacaacc cgcaatatgc
     2881 cgagctgttc cactcctcct gctgggggtc cgactacgag gcctacgacc tgataaacat
     2941 gcagtcgtgc ctgatcatgg gtgaatatct tctggcggga cgcctgccgc ggcagaacca
     3001 ggacggccgc tggtacatga tgcgggggca ctatgccgaa ttgaagcggc agatggggtg
     3061 ggtcgaattc cagatcgagc aaaatatcga tcggctgaaa aacgattcgg agttcgtcta
     3121 ccagatctcg gaataccagg acctgctgga caaggtgctc aagaccgaga ccagcgaccg
     3181 gccttccatt atttccatcg ccaaggatca gggcgagcgc gccggcgatc tggagcggct
     3241 tcaccagctg gcccaggagg atttcgaggc gcgggcgtcg gcgatcaaat ccagagaatt
     3301 atgtcgcctg ctggcctgcg accagatctt tgagccggcc gatcagctcc gtcgcctcag
     3361 aagccgggaa atactcggga aagtccgttc gttcgagcac gcctttcggc ccgatcggga
     3421 cgcccgcgag gcgatcgaga gggacatgga ggattgggac gccgagctgc tcgaagagat
     3481 aaatgcgaag ccgaagcaat atgcccggac ggtgaaggcg cggaagaatg acagccgcac
     3541 cctggcccat ctgcaatggg cggcccgtca gcgcgtgcag atcaaccacc ggatcgtgtt
     3601 cgtcaccggt gaccgggcga tctacgacgt ctaccgcaag cgggcggccg aaggcgcgga
     3661 aggctcgccc ttcctccttc gcccgctcgt ccaatatgct cccttgttca atccttccag
     3721 cgccgacagc tgggtggccg agcgcgaaaa ggcgttcgcc ctcgtccggg gcgccctcga
     3781 ggccgccatg gtcgcgctca gcctcaatct gttcggaaag ggcgagccca aggcgctgac
     3841 ccgcgcccgg gaccatttcg tgctcgcctc ccagaagccg ctcaagaacc tcaaatggct
     3901 cagcggattt ccggcgcttt cggaggagcg gctgctcaat gagcaggcgc cgaagctcac
     3961 ggcgctggcc gctccgctgc agcgcgtcga aaggctgatg ctcggagcct atccgacgct
     4021 gctcgcgcgg cggctcgaaa gccgggagcg gcgggaggcg ttcctgaagt cggtcgcctc
     4081 gacccacggc gccgccctgg cggaggcgct gcagtccagc ctcgaggagg cgtcgcgcac
     4141 cgccttcatg tactcgctcc cgttcatgcc cgaggagatc gtcaagctcc tggaccggat
     4201 caagcgcggc gacggcaggc tgccgcgcct gctgatcggg gtacgcctga cctttccggc
     4261 ggagcagccg gaccgtcccg acgagcagtt gacccggaag gacatcatca ggaggctcac
     4321 caaagccgac tgccagctcg acaccctggt cgaacgcctg gcggaggtgc ccgcgcgcgt
     4381 cttcgcgctt gccgccctgc tcgccttcgg agtcgagaag tgggaggcgg cggcgcgcta
     4441 tgccgacctg tcggcgacgg cctcgctcta cgaccggcct aaggacggtc cgaccggcga
     4501 gagcggggac catttcgagc ttttgtacat gaaggcggtg gggctgcgct tcaccctgtt
     4561 cagccgcgag cccaatcccg cccatcatta ttacgaccgc tgggcgcagt ggctgctcga
     4621 agccgaggac gcgctcgaat cctgcatcgt ccagcatcgc ggggaaaacg agctctcccg
     4681 cgagttgcgc gccatttcgg agcgcgccgc gatccggctc gcctattgcg aatggtatgc
     4741 gttcggacgc ctgagcgaga cccgcatggc ggaggaggcg cgggaggtcg cgctgcgcgc
     4801 gctcggccag gcggtggaag acctcctggc ctgctatcac aagcgcgacg ccgccgcggc
     4861 gcgggctctg gacgacggcc attcgggccg gcatctgagc gcggatacgc tccattgggc
     4921 gacgctgcag ttccacatca acaccgccgc cgcgatcctc gtcgcgaacg agctggcgct
     4981 caaatggccg gagctggcgg accgggtgga aaagtcgatc gagccgctgc cgcggccgga
     5041 cgactggccg aggacggagg acctgccgcc gatgagccgc gcctatatcc tggccgccac
     5101 gtcccgctgg cgggagctgc gcgcgatccg cacgcgcggg ctcagcctgc cgctcgaccg
     5161 ggtcgtcatc cgcggcctcc aggcgcgcgt tcggcgctag ccgccgggcg ccgcggcggt
     5221 cgacgggccc gcgcgggggc ggcgtccgcg cctcaagtca cccagaggta gaggccgatc
     5281 atcgccggga gggcgatcag gaagctggcc aggccgtagc tcagggtccg ggcggcgggg
     5341 ggttcgccgg tggccacggc tcagcccccc tcgccgggcc gccgggcccg ggaagggtcc
     5401 gccgggccgc ggccttccgg caccggcaag gcgagctgcg cgcgctccgg ctcgcgcgcc
     5461 agggcggcgc tcgcggccgc ggcggccttg acgctgtagc gcccggcgag gtcgagatgg
     5521 gcgagcctcg cctcggagct tccggcctgc ttcgcattcg ccgccgaggc gcgcttgcgg
     5581 gcgagccaat aggcctgttc catgggggac ccttcgtcct gcaagcggga gcgctcgaag
     5641 tctctcggtc acggggcgct tgcggaaaaa aaggaccggg tgatggagcc tatatggggg
     5701 gccggcggcg gatagcgagg ggcgccgggc aaatcgggcc cggcgccccc gcctcggccg
     5761 ctagcggcgg cccctctcgt aggtctgcag gcagtggtcg gtgacgctcg ccgtgcaggc
     5821 cggatagctg cgcggcgcgg cgggctcgcc ggtggccggg tcgagctccg ggccgcccat
     5881 agcggcgccg gcggcggtgc cgttccagcc ggccgggacg acggccgcgg ccgagatcac
     5941 cgggatcccg cgcgcatcgc gctcgggcgc ggaattgccg ggctcgacga ccgctccggc
     6001 ggtgaccggg ccggcctcgg ccagggtcgg cgccgaatag gccccgctcg agccgccctc
     6061 ggtctcgaag gcggtggtgg cgacgagatc gccctcatag gcggtctgct cggcggtgac
     6121 gtcgatctgg gcgaaggccg tgccggagat cagcagggcg agcgcgaaag cggtggattt
     6181 catccgttat cctcctctgg tcgaccctgc gcttcgcggc ggccggcgac tcgggccatg
     6241 ccgggcgggc aggggttgcg aggggggaac gctcgagccc gcgcttcttt ccggcggcgg
     6301 ccgcgcccgg cagctttgcc gggccgctcc gctcgcccgg ctcgctcagc cgctgcgccc
     6361 ggcccgccgc gcctccatgt cggcgatccg ccagccgccg aacagggcgg ggccgggatt
     6421 gtgggccggc cgcccgctcg ccctcgacca gccggtgacc gtctcgagcg gcggaagcgg
     6481 cggcggcggc ggctcgtcgg cgtggcgcca gtcgccggtc tcctcgaagc gctcggcgcg
     6541 cctcagcgcc gccgcctccg cctcgacctg cttctcggcc cgccacatcg cctgcaggcg
     6601 caggctgtag atctcgtcgc tctcgccgcg ccgccttcgc cggtgcggct gctcccagcc
     6661 ctggttgacg ctcttctcgt gcaggcagag cagttggaag gcctggtccc agctcagctt
     6721 cggcatcggc gcctccgggc agttgcgcca gcggtcgcgc gcgtggctga agggcgaggc
     6781 ggccctcaac gattccatct tcagccgctc ccagcccatc ttcagggcca gccggaattc
     6841 gcgggcgaac gccgggtccc gccgccgccg ccggtagaag gcgcgcgccg aggcccccgc
     6901 cgccgccgcc gacagccgga cgtt
//
LOCUS       DASVQZ010000233         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_916951, whole genome shotgun sequence.
ACCESSION   DASVQZ010000233 DASVQZ010000000
VERSION     DASVQZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916951"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VF547_06320"
     CDS             <1..748
                     /locus_tag="VF547_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX8262471.1"
                     /translation="RALRRISTYDVETRREIAVPVADLGDVPVQGVSPAEAFAPIRDA
                     AAAAVARHPLTLLLGGNNAVTRPAVHALGLSLDRVGLITLDAHFDMRGTEGGPMNGNP
                     VRCLLEDGLPGWNVCQIGLAPFANTAAMHRDAEAAGAGLFTIADCKARGIHAVLDEAL
                     ARLAHKEAIAVDFDIDVIDRSQLPGAPGARPGGMPVDMFFAAARRLAVEPQVRLVDLT
                     EFDPSLDVSDISALTAGRWVAEILSGFAAR"
     gene            877..>1519
                     /locus_tag="VF547_06325"
     CDS             877..>1519
                     /locus_tag="VF547_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262472.1"
                     /translation="MQLLKEGRYLDALDSAEAAAAKALFPLQTLEQMRPMIGSYAPLP
                     PLRPGSPPLPSDMAAKLQHAAAEDALPLILKAATRTDIVILNEAHHSPRDRAFALEVA
                     RALRPLGYTVLAVEAFTNHPPVITALAREGFPRLNSGLYTAEPVFGDFVRQALALGYT
                     PIAYEQTAAQQERGEPGTPGREQAQAENLAAVVRANPGRKLFVYVGFSHVAEAP"
BASE COUNT          213 a          567 c          505 g          234 t
ORIGIN      
        1 gcgggcgctc aggcggatca gcacctacga tgtcgagacg cggcgcgaaa tcgccgtgcc
       61 ggtagcggat ctcggcgacg tgccggtcca gggcgtctcc ccggctgagg cgttcgcgcc
      121 gatccgcgac gccgccgccg ccgccgtcgc gcggcatccg ctcactctgc tactcggcgg
      181 caacaatgct gtcacacgcc ccgccgtcca tgcgttgggc ttgtcgctcg acagggtcgg
      241 gctgatcacg ctcgacgcgc atttcgacat gcgcggcacc gaaggcggac cgatgaacgg
      301 caaccccgtt cgttgcctgc tggaagacgg cctgcccggc tggaacgtct gccagatcgg
      361 cctcgccccc ttcgccaaca ccgccgccat gcaccgcgac gccgaagcgg cgggggccgg
      421 cctcttcact attgccgatt gcaaggcgcg cgggattcac gccgtgctcg acgaggcgct
      481 ggcccgcctc gcccacaagg aggcgatcgc ggtcgatttc gacatcgacg tgatcgaccg
      541 atcccagctc ccgggcgcgc ccggcgcccg gcccggcggc atgccggtcg acatgttctt
      601 cgccgccgcc cgccgcctcg cggtcgagcc gcaggtgcgg ctcgtggacc tgaccgaatt
      661 cgacccgagc ctcgacgtgt ccgacatctc ggccctcacc gcgggacgat gggtggcgga
      721 gatcctgtcg ggcttcgccg cccgctgaag gaggccgcct tgatgcgtct gccgcgacta
      781 gtcgccgccg ggtccgcttt tcttctcgcc gggggcgccc cgccgccctc ggccggcccc
      841 gccaagccga cgccggttcg ggaggcaggc gagccgatgc agcttctgaa ggaaggccgg
      901 taccttgacg ccctggacag tgccgaggct gccgcggcga aggccttgtt cccgttgcag
      961 acgctggagc agatgcgccc gatgatcgga agctacgcgc cgctgccgcc gctccgcccc
     1021 ggttcgcctc cgctcccctc cgatatggcc gcgaagctgc agcatgcggc tgccgaggac
     1081 gcgcttcccc tcatcctcaa agccgccacc cggacggata tcgtcattct gaacgaggcg
     1141 caccacagcc cgcgcgaccg cgccttcgcc ctggaggttg cgcgggcgct gcggccgctg
     1201 ggctacaccg tgctggcggt ggaagcgttc accaatcatc cgccggtgat cacggctctc
     1261 gcgcgtgaag gtttcccgcg actaaattcc ggcctctaca cggcggagcc cgttttcggc
     1321 gactttgtcc gccaggcatt ggcccttggc tacacgccga tagcctacga gcagaccgcc
     1381 gcgcagcagg agcgcggaga gcccggcacg cccggccgcg aacaggcgca ggccgagaac
     1441 ttggcggcgg tcgtccgcgc caatccgggc cgaaagctgt tcgtctatgt cggcttcagc
     1501 catgtcgcgg aggcgccgg
//
LOCUS       DASVQZ010000234         6115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_625845, whole genome shotgun sequence.
ACCESSION   DASVQZ010000234 DASVQZ010000000
VERSION     DASVQZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625845"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VF547_06330"
     CDS             complement(<1..1119)
                     /locus_tag="VF547_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8262473.1"
                     /translation="MFLKIARFELRYQVRNPVFWVAAILFLLLTFGAMASGNVQIGSG
                     GNIRKNSPVALAQTQIILSLFFMFVTTAFVANVVVRDDDTGFGPIIRSTPITRLQYLG
                     GRFAGAVAAAALAFLFIPLGSWIGSLMPWVDAETLGPNLLSHYAYGLLVFGLPNMLVT
                     AAIFFTAATLTRSMMWTYVAVIVFFLGWLILTGIAQAKPELRDTMALIEPFGAGAFRD
                     ATRYWTAAESNALMPAVEGIILLNRLIWLAVGTAFLALGIARYRFAEPGLSKKRRKDE
                     AAEAEPAAASAPMQPLPGRSGSAAAAQLWVRTKFEMKQVFKSPAFAVLVALGMINTGA
                     SLWFSREMFGTPILPVTTAMIPIVAGSFSLFPIIIAIYY"
     gene            complement(1132..2010)
                     /locus_tag="VF547_06335"
     CDS             complement(1132..2010)
                     /locus_tag="VF547_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8262474.1"
                     /translation="MLELADVSHVYPNGTRALDRVTLSIPRGMYGLLGPNGAGKSTLM
                     RNVATLQTPTEGTIRFGDIDVIAEPEKLRRTLGYLPQDFGVYPRVSAYDMLDHMAVLK
                     GIASAADRKATVEALLHQVNLWDVRKKALAGFSGGMRQRFGIAQALIGNPGLIIVDEP
                     TAGLDPEERNRFLNLLAEIGENVVVILSTHIVEDVSDLCPRMAVLANGRIQLEGAPLD
                     LIRATRGRIWMKVIERGEIDRYRETYELISTRLFAGRTVIHVLANSDPGDGFASVEGG
                     LEDVYFSTLAQSRRAA"
     gene            2132..3508
                     /locus_tag="VF547_06340"
     CDS             2132..3508
                     /locus_tag="VF547_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8262475.1"
                     /translation="MRKLAPLLPLLLLAMPATAATPREIADQVGQDRIRAVVEKLVSF
                     GTRHTLSSQTDPKRGIGAALKWTEAEFGRYSRACGGCLRVVLPSDVVTGRRVPSPTKV
                     TNVVAIQRGTVDPGRVVIISGHIDSRVTDVMDFTKDAPGANDDGSGTAAVIEAARVLS
                     KHKFPYTIVYAALAGEEQGLLGGKILADYAKGQRWEVLANLNNDIIGGSCGSDGVCDD
                     AHVRIFSEGPRWQGHEDLAARIRSLGGENDAPSRNISRWLDGLADDLSLGLDVRQIWR
                     NDRFGRGGDHTEMLNAGYPAVRITVAIENYNWQHQDLRVENGIEYGDTIDKMDFPYLT
                     KVVKLNVAALAALASAPPPPEPKVEGAVSSDTTLTWGLGKGAAAYIVRWRPTDAPNWT
                     NSLRVDACMILPSNPPSGSCKAVLKGVRVDDFVFGVSSVSADGYESPVASAVPGGAFR
                     PYVPPPPQ"
     gene            3609..6017
                     /locus_tag="VF547_06345"
     CDS             3609..6017
                     /locus_tag="VF547_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX8262476.1"
                     /translation="MARKPQRAGLPSRQQILDFIASSDTPAGKREIARAFGLSGHDKI
                     ALKTLLRDMADEGLIDASPGRAFHQAGGVPKVTVLRIVDIDDSGTAWAVPERWEADLP
                     EPRVRVLERKGRGSALAVGDRVLARTEQRGNGHVAHPMKKLAKGSELVLGVVHQEGES
                     FWLRPVEKKERRELPISDLGRATAGDLVLAEKAGRPPRLTARVSEVLGDPFAPRSFSL
                     IAIHKHGIPHEFPDSVIEEAERVSGQDLGEREDLTHMPIVAIDPADARDHDDAVWAAP
                     DEDPANEGGWRAVVAIADVSFYVRPGSALDKEARKRGNSVYFPDRVVPMLPETLSAEI
                     CSLKEGEDRAALACHLQVSKEGQLKSWRFSRARVRIAANIAYEDAQAAIDALEFLPGT
                     GRETARSAAEGSPLPGPLHHASDGPPPRSGEEPIELFSSPCSMPEIEAEPPPVDPAIV
                     ESTLKPLWACWRALYSARAKRGPLELDLPERQVVLDEKGRITSVAPRERLDAHKLIED
                     YMIAANVAAAKALEAKKAPVMYRDHEPPGREKLVALKDYLKTFGVEFALGQVVRPATF
                     NHIIERIGDADFRPQVMEQILRSQTQAYYSPDNKGHFGLALGSYAHFTSPIRRYADLV
                     VHRSLVRAYRLGDGGLSDQEAEAMEVTGELISQLERRAMEAERETLDRYVAAYLSERV
                     GDLLDCRITGVQPFGFFATVEGLGGDGLVPVSTLGSDYFRYDEASQSLVGDDSGERFS
                     PGQRLKLRLVEANPVSGGLRFELPDAGYSAAAPRRRPRRDGDDRPRPQGRRGRPSNIR
                     HRGR"
BASE COUNT         1020 a         2021 c         2172 g          902 t
ORIGIN      
        1 ataatagatg gcgatgatga tcgggaacag gctgaacgac ccggcgacga tcgggatcat
       61 cgcggtggtc accggcagga tcggcgttcc gaacatctcg cggctgaacc agaggctcgc
      121 cccggtattg atcattccca gagcgaccag gaccgcgaag gcggggctct tgaagacctg
      181 cttcatctcg aacttcgtgc gcacccacag ctgggcggcc gcggccgagc cggagcggcc
      241 gggcagcggc tgcatcggcg ctgaggcggc ggccggctcg gcctccgccg cctcgtcctt
      301 ccggcgcttt ttcgaaaggc cgggttcggc gaagcggtag cgggcgatgc cgagcgccag
      361 gaaggcagtt ccgacggcga gccagatcag gcggttgaga aggataattc cctcgaccgc
      421 cggcatcagg gcgttggatt cggcggcggt ccagtagcgg gtggcgtcgc ggaaggcgcc
      481 cgccccgaac ggctcgatca gagccatcgt gtcgcgcagc tcgggcttgg cctgggcgat
      541 cccggtgagg atcagccagc ccaggaagaa gacgatgaca gcgacgtagg tccacatcat
      601 cgaccgggtg agggtcgcgg cggtgaagaa gatggcggcg gtgaccagca tgttgggcag
      661 gccgaagacc agcagcccat aggcgtagtg cgacaggagg ttgggtccga gggtctcggc
      721 gtcgacccac ggcatcagcg agccgatcca ggatccgagc gggatgaaca ggaaggccag
      781 ggcggccgcc gcgacggcgc cggcgaagcg tccgcccaga tattgcaggc gggtgatcgg
      841 cgtcgagcgg atgatcggcc cgaagccggt atcgtcgtcg cgcaccacca cgttggcgac
      901 gaaggccgtg gtcacgaaca tgaagaacag gctgaggatg atctgggtct gggcgagggc
      961 gacagggctg ttcttgcgga tgttgccgcc cgagccgatc tgcacgttgc cggaggccat
     1021 cgctccgaag gtgagcagga ggaagaggat cgccgccacc cagaagaccg ggttccggac
     1081 ctgatagcgc agctcgaagc gcgcgatctt gaggaacatc ggattgctcc cctaggccgc
     1141 gcggcgggat tgggcgaggg tcgagaaata gacgtcctcg agcccgcctt cgaccgaggc
     1201 gaagccgtcg ccgggatcgg agttggcgag gacgtggatg acggtccggc cggcgaacag
     1261 cctcgtcgag atcagctcat aagtctcccg ataccggtcg atctcgccgc gttcgatgac
     1321 cttcatccag atccgcccgc gggtcgcgcg gatgaggtcg agcggagcgc cctcgagctg
     1381 gatgcggccg ttggcgagca ccgccatccg cgggcacagg tccgagacgt cctcgacgat
     1441 atgggtggag aggatgacga cgacattctc gccgatctcg gcgagcaggt tcaagaagcg
     1501 gttgcgctcc tcggggtcga ggccggcggt cggctcgtcg acgatgatca gcccgggatt
     1561 gccgatcagc gcctgggcga tgccgaagcg ctggcgcatc ccgcccgaga agccggccag
     1621 cgccttcttg cgcacgtccc acaggttgac ctggtggagg agagcctcga ccgtggcctt
     1681 gcggtcggcg gcggaggcga tgcccttgag caccgccata tggtcgagca tgtcgtaggc
     1741 cgaaacgcgc ggatagactc cgaaatcctg gggcagatag cccagggtgc ggcgaagctt
     1801 ctcgggctcg gcgatgacgt cgatgtcgcc gaagcggatc gtcccctcgg tcggggtctg
     1861 cagggtcgcg acgttgcgca tcagggtcga cttgccggcg ccgttcgggc cgagcaggcc
     1921 gtacatgccg cgggggatgg agagggtcac ccggtcgagg gcgcgggtgc cgttcggata
     1981 gacgtggctg acgtcggcaa gctcgagcat tcgaagatat tccccaaccc ggtgttcggg
     2041 ccgaggtctg ccagagcgtc tcgggccttg taaagcagcg gcgcccgccc gcgcttgcga
     2101 attcgggccg acaggttcag gggccgacgc catgagaaag ctcgcaccgc tcctcccgct
     2161 cctcctcctc gccatgcccg ccactgccgc gactccccgc gagatcgccg accaggtcgg
     2221 tcaggaccgc atccgggccg tcgtcgagaa gctggtgagc ttcggcacgc gccacacctt
     2281 gtcctcgcag accgacccga agcgcggcat cggcgcggcg ctcaaatgga ccgaggcgga
     2341 gttcggccgc tattcgcggg cctgcggcgg ctgcctcagg gtcgtcctgc cgtccgacgt
     2401 cgtcacgggc cggagggtgc cttcgccgac gaaggtgacg aatgtggtcg cgatccagcg
     2461 cggaaccgtc gatccgggcc gcgtcgtgat catttccggc catatcgaca gccgggtcac
     2521 cgacgtcatg gacttcacca aggatgctcc cggagccaat gacgacggca gcggcacggc
     2581 ggccgtgatc gaggcggcga gggtcctttc gaagcacaaa ttcccctaca cgatcgtgta
     2641 tgcggcgctc gccggcgagg agcagggcct gctggggggc aagatcctgg ccgattatgc
     2701 caaggggcag cgctgggaag tgctggcgaa cctcaacaac gacatcatcg gcggaagctg
     2761 tggttcggac ggggtctgcg acgatgcgca cgtccggata ttctccgaag ggcctcgctg
     2821 gcaggggcat gaggacctcg ccgcacgcat ccgcagcctc ggcggcgaga acgacgcgcc
     2881 gtcgcgcaac atctcgcgct ggctcgacgg tctcgcggac gacctcagcc tcggcctcga
     2941 cgtccgccag atctggcgca acgaccgctt cggccgcggc ggcgaccata cggagatgct
     3001 caacgcgggc tatccggcgg tgcggatcac ggtcgcgatc gagaattaca actggcagca
     3061 ccaggacctg agggtcgaga acgggatcga atatggcgac accatcgaca agatggactt
     3121 cccctatctg accaaggtgg tgaagctgaa cgtggcggcg ctggccgctc tggccagcgc
     3181 tccgccgccg cccgagccca aggtggaagg cgccgtctcg agtgacacga cgctgacctg
     3241 gggtctcgga aaaggcgcgg cggcgtatat cgtccgctgg cgtccgaccg acgcgccgaa
     3301 ctggaccaac agcctgaggg tcgacgcctg catgatcctt ccttccaatc cgcccagcgg
     3361 cagctgcaag gcggtgctca agggcgttcg agtcgacgat ttcgtgttcg gcgtctcctc
     3421 ggtttcggcc gacggctatg aatcgccggt cgcttcggcc gtgccgggcg gtgcgttccg
     3481 gccttatgtg ccgcctccgc cgcaatgatc ctcgccccgg acctgatccg gggtccacct
     3541 tctcttcaac cgggccggca agaaggtgga tgccggatca agtccggcat gacggggttt
     3601 gaacggcgat ggcgagaaag ccccaacgag ccggcctccc gagccgccag cagatcctcg
     3661 acttcatcgc gtcgagcgac acgccggccg gcaagcgcga gatcgcgcgc gccttcggcc
     3721 tgtcgggcca tgacaagatc gccttgaaga ccctgctgcg cgacatggcc gacgaagggc
     3781 tgatcgacgc ctcgcccggg cgggccttcc accaggcggg cggggtgccg aaggtgacgg
     3841 tgcttcgaat cgtcgacatc gacgacagcg gcacggcctg ggccgttccg gagcggtggg
     3901 aggccgatct gccggaaccg cgcgtgcggg tgctcgagcg gaagggcagg ggttccgcgc
     3961 tggcggtggg cgaccgagtg ctggcccgca ccgagcagcg cggcaacggc catgtcgccc
     4021 acccgatgaa gaagctcgcc aagggcagcg agctggtcct cggcgtcgtc catcaggaag
     4081 gggaaagctt ctggctgagg ccggtcgaga agaaggagcg gcgcgagctg ccgatatcgg
     4141 atctcggtcg ggcgacggcg ggcgacctcg tcctcgccga gaaggccggc cggccgccgc
     4201 gcctcaccgc gcgggtctcg gaggtgctgg gcgacccctt cgcgccgcgg agcttcagcc
     4261 tgatcgccat ccacaagcat ggcatcccgc acgaatttcc ggactcggtc atcgaggagg
     4321 ccgaacgggt ttcggggcag gacctgggcg agcgcgagga cctcacgcac atgcccatcg
     4381 tcgccatcga cccggccgat gcccgcgacc atgacgacgc ggtctgggcc gcgccggacg
     4441 aggatcccgc caacgaaggc ggctggaggg cggtcgtcgc catcgccgac gtcagcttct
     4501 acgtccgtcc cggctcggcg ctcgacaagg aggcgaggaa gcgcggcaac agcgtctatt
     4561 tccccgaccg ggtggtgccg atgctgcccg agaccctgag cgcggagatc tgctcgctga
     4621 aggagggcga ggatcgcgcg gctctggcct gccacctgca ggtgagcaag gagggccagc
     4681 tcaagagctg gcgcttcagc cgggcccgag tccggatcgc ggccaacatc gcctacgagg
     4741 acgcgcaggc ggcgatcgac gccttggagt tcctccccgg aacggggagg gagaccgcgc
     4801 gaagtgcggc ggaggggtcc ccgcttccgg gccccctcca ccatgcttcg gatggtcccc
     4861 ctccccgttc cggggaggag ccaatcgagc tcttctcctc cccctgctcg atgccggaga
     4921 tcgaggcgga gccaccgccc gtcgatccgg cgatcgtcga atccaccctc aagccgctct
     4981 gggcctgctg gcgcgccctc tattcggcgc gggcgaagcg cgggccgctc gagctcgacc
     5041 tgcccgagcg ccaggtcgtg ctcgacgaga agggccggat cacctcggtg gccccgcgcg
     5101 agcggctcga cgcgcacaag ctgatcgagg attacatgat cgccgccaac gtcgccgccg
     5161 ccaaggcgct cgaggcgaag aaggcgccgg tcatgtaccg tgaccacgag cctccgggac
     5221 gcgagaagct ggtcgccctc aaggattatc tcaagacgtt cggcgtcgag ttcgcgctgg
     5281 gccaggtggt tcggccggcg acgttcaacc acatcatcga gcggatcggc gacgccgact
     5341 tccggccgca ggtgatggag cagatcctgc gcagccagac ccaggcctat tattcgcccg
     5401 acaacaaggg gcatttcggc ctggcgctcg gctcctacgc ccatttcacc agcccgatcc
     5461 ggcgctatgc cgacctcgtc gtgcatcgct cgctggttcg cgcctaccgc ctcggggacg
     5521 gcgggctcag cgaccaggag gccgaggcga tggaggtgac gggagagctg atctcgcagc
     5581 tggagcggcg ggccatggag gccgagcgcg agaccctcga ccgctacgtc gcggcctatc
     5641 tttcggagcg cgtcggcgac cttctcgact gccggatcac cggcgtccag ccgttcggct
     5701 tcttcgcgac ggtcgagggc ctcggcggcg acggcctggt cccggtctcg acgctcggca
     5761 gcgactattt ccgctacgac gaggccagcc agagcctggt cggcgacgac agcggcgagc
     5821 gcttcagccc cggtcagcgg ctcaagctgc gcctggtcga ggccaatccg gtttcaggcg
     5881 ggctgaggtt cgagcttccc gacgccggct actcggccgc ggcgccgcgc cgcaggccgc
     5941 ggcgcgacgg ggacgaccgg ccacggccgc agggccgccg cggccggccc tcgaacatcc
     6001 gccaccgggg acgatagcgg ctttcctcag ccgatccgcc agatcacccg gccgtcatag
     6061 gtcgaccggc ccgcccggcc gcgcgaatcg cgcgccgggg tgaagcgcgc ccgcg
//
LOCUS       DASVQZ010000235         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_625960, whole genome shotgun sequence.
ACCESSION   DASVQZ010000235 DASVQZ010000000
VERSION     DASVQZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625960"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF547_06350"
     CDS             <1..285
                     /locus_tag="VF547_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262477.1"
                     /translation="DNWSGRGRFETYRWYATFATRTPVLPGAYEMVAPLNGPWTAVET
                     SSARTNPAGFRAALADADQLGFVLGGGDGYGHGIFATGRARLVVTDFRVE"
     gene            complement(343..534)
                     /locus_tag="VF547_06355"
     CDS             complement(343..534)
                     /locus_tag="VF547_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262478.1"
                     /translation="MAGSAIAARIRDGRPPVRTPGLALRDPPRTYRVCAALAAAAALA
                     GAAGWTLAEADDPPVRWRF"
     gene            554..1060
                     /locus_tag="VF547_06360"
     CDS             554..1060
                     /locus_tag="VF547_06360"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8262479.1"
                     /translation="MTPEISFRQAQARDIPRLAELRWQMRMEGGEAPGDGREAFIRAC
                     AAFYERTMAPGLQTHFVAEQDGRIVATVSLHEVEMLPRPGRIEDRFGCITNNFSEPGA
                     RGRRIASALLGHVVGHAEANDLELLIVWPSERAVPFYERLGFQWENEVMELRLRDYVA
                     IARSGDSP"
     gene            complement(1167..1796)
                     /locus_tag="VF547_06365"
     CDS             complement(1167..1796)
                     /locus_tag="VF547_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX8262480.1"
                     /translation="MHCVKCGTAIPEGAAFCPKCGASVDGGAAAAGTAAPAQPAQPRK
                     KSNKTLGCIAAAVGILVLLFVIGSFLPDKPAGSEAPGAAGKDGAPAELPLAVSAAELF
                     QAFQGNEASAQSYFGKRKLLVSGTVEKVMLDFMDDPVVLLRTPNRFMSAQAALADDAK
                     GEAGNLGPGDKVEMLCEDVREVMGTPMLKDCRTAPPGTKSQKVKWADKK"
BASE COUNT          263 a          707 c          674 g          282 t
ORIGIN      
        1 gacaattgga gcggccgcgg gcggttcgag acctaccgct ggtacgccac cttcgccacc
       61 cgcaccccgg tccttcccgg cgcctacgag atggtcgctc cgctgaacgg cccgtggacc
      121 gcggtcgaga ccagctcggc ccgcaccaac cccgccggct tccgagccgc cctcgccgac
      181 gccgaccagc tcggcttcgt cctcggcggc ggcgacggct acggccatgg gatcttcgcc
      241 accggccggg cgcggctggt cgtcaccgac ttccgggtcg agtgagcagg aaggtcgcga
      301 cggcgaggag gagcgcgaag gccgcgagcg tgatcgcgcc catcagaagc gccagcgcac
      361 cggcggatcg tccgcttcgg cgagcgtcca gccggcggct ccggccagcg ccgccgccgc
      421 ggcgagggcg gcgcagaccc ggtaggtccg cggcggatcg cgaagcgcga gcccaggcgt
      481 tcgaaccgga ggccgtccgt cgcggatccg ggcggcgatt gcggagccgg ccattctgac
      541 ctagccccgc gccatgacgc ccgagatttc cttccggcag gcgcaggcgc gggacatccc
      601 ccgcctggcc gagctgcgct ggcagatgag gatggagggg ggcgaggcgc cgggcgacgg
      661 gcgcgaggcg ttcatccggg cctgcgccgc cttctacgag cgaacgatgg cgccagggct
      721 ccagacccat ttcgtcgccg agcaggacgg ccggatcgtc gccacggtct cgctccacga
      781 ggtcgagatg ctccctcgcc ccggccgcat cgaggaccgt ttcggctgca tcaccaacaa
      841 tttttccgag ccgggcgccc gcggccgaag gatcgcctcc gccttgctcg gccatgtcgt
      901 cggccatgcc gaggcgaacg acctcgagct gctgatcgtc tggccgagcg agcgggcggt
      961 gcccttctac gagcggctcg gattccagtg ggagaacgag gtgatggagt tgcggcttcg
     1021 cgactatgtc gcgatcgccc gcagcggcga ttccccttag cgggccatcc tccgcggcag
     1081 ccgtcgcgac ggcgcgatcg aggaaggggc cgaagccctg cgcaggtcgc cgccgtcggc
     1141 accggctggg cccgccggtc cccggctcac ttcttgtccg cccacttcac cttctggctc
     1201 ttggttccgg gaggcgcggt gcggcaatcc ttcagcatcg gcgtccccat cacctcccgc
     1261 acgtcctcgc agagcatctc gaccttgtcg ccgggaccga gattcccggc ctcgcccttg
     1321 gcgtcgtcgg cgagcgccgc ctgggccgac atgaaccggt tgggggtgcg cagcaggacg
     1381 accgggtcgt ccatgaaatc gagcatgacc ttctcgaccg tgcccgagac cagcagcttg
     1441 cgcttgccga aataggattg ggcgctggcc tcgttgccct ggaaggcctg gaagagctcg
     1501 gccgcgctga ccgcgagcgg cagctccgcc ggggcgccgt ccttgccggc ggcccccggc
     1561 gcctccgatc ccgccggctt gtcgggcagg aagctgccaa tgacgaagag caggacgagg
     1621 atcccgaccg cggcggcgat gcagcccagg gtcttgttgg acttcttcct gggctgggcc
     1681 ggctgggccg gagccgcggt tccggcggcc gcggcgccgc cgtccacgga ggccccgcac
     1741 ttcgggcaga aagcggcccc ctcgggaatg gcggtcccgc acttcacgca atgcattggc
     1801 caattctcct tgtctgctcc gaagactgcc cgagccgccg gggcgtgaca acccgcccga
     1861 ccggcccttt tgccgctcga acagtcggcc gagcgcctcg gtcgctgtcg accgccttgc
     1921 cggagg
//
LOCUS       DASVQZ010000236         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_529900, whole genome shotgun sequence.
ACCESSION   DASVQZ010000236 DASVQZ010000000
VERSION     DASVQZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529900"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF547_06370"
     CDS             <1..475
                     /locus_tag="VF547_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262481.1"
                     /translation="GIRSDPAWAGGDYSSQPVQGLRTAVSLLQVAGFAPLYLQKAYPT
                     REAADAYILDRVAKDLPTRDANDLVYQLEASRTYNPWPRLERIRAPITWVNSADDFIN
                     PAEYGIAEDAARRLRTGRYILLRATSDTRGHGTHTWAKFWRQELIDLLARTEDPS"
     gene            716..1597
                     /locus_tag="VF547_06375"
     CDS             716..1597
                     /locus_tag="VF547_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262482.1"
                     /translation="METLRSLVAAELAEPVDPRVSAMAEAIAAKYPGAARAVLFYGSC
                     LRQRELDGLMLDFYLIVSDYRSAYGRSWLALANRLIPPNVFPFEHDGLVAKYAVLDER
                     DFYRLNGLETLNVSVWARFAQPSRLVWSADEKAAAAAVVSVARAAPTLLAAAAPSLPD
                     PQEPLDMWRGAFALTYGAELRAERRGREGSVVDSDPERYLRFSRPAMAAVPGDGPLAR
                     AARRPWWKRRLVGKALTVVRLMKASGTYAGGIDYLAWKINRHAGTKIELKPWQRRWPL
                     VAAIGLLPRLLRRGAIR"
BASE COUNT          236 a          563 c          579 g          249 t
ORIGIN      
        1 ggggatcagg agcgacccgg cctgggccgg cggcgattat tcgagccagc cggtccaggg
       61 cctgcgcacc gcggtcagcc tgctccaggt cgccggcttc gcgccgctct atctccagaa
      121 ggcctatccg acccgggagg cggcggacgc ctatatcctc gaccgggtgg cgaaggacct
      181 gccgacccgc gacgccaacg acctcgtcta ccagctcgag gcgtcgcgga cctacaatcc
      241 ctggccgagg ctcgagcgga tccgggcgcc gattacctgg gtcaactcgg ccgacgactt
      301 catcaacccg gccgaatacg gaatcgcgga ggacgcggct cggcggctca ggaccgggcg
      361 ctacatcctc ctccgggcca ccagcgacac gcgcggccac ggcacgcaca cctgggccaa
      421 attctggcgg caggagctga tcgacctgct cgctcggacg gaggatccga gctgaatgga
      481 ccggccctcg gacgctgtgc gaggccggtt ttgagagaag gcacgaaggc acgaagcgcg
      541 cgcagcgccg tgatcgccgg cgaccgtgat tccgaggaat ttgatcggcc gctccgcggc
      601 cgcttcgtgc cttcgtgcct tctataagat ccgaaacgtt cggcgccggc cgggcgcggg
      661 gccgccgacc ctgcgctgcc ctagcttcgc cgcgttggcg gtttatagga gcgccatgga
      721 gacgctgaga agcctggtcg cggccgagct ggccgagccg gtcgacccgc gggtgagcgc
      781 gatggcggag gcgatcgcgg cgaaatatcc cggcgccgcc cgggcggtgt tgttctacgg
      841 ctcctgcctg aggcagcgcg agctcgacgg gctgatgctc gatttctacc tgatcgtctc
      901 cgattaccga tccgcctacg gccgcagctg gctggcgctg gcgaaccggc tgatccctcc
      961 caacgtcttc ccgttcgagc atgacgggct ggtcgccaaa tatgcggtgc tggacgagcg
     1021 cgacttctac cggctcaacg ggctcgagac cctcaacgtt tcggtctggg cccggtttgc
     1081 ccagccaagc cgcttggtct ggtcggccga cgagaaggcg gcggcggccg cggtggtctc
     1141 cgtcgccagg gcggccccga cccttctcgc cgccgccgcg ccgtccctgc ccgatcccca
     1201 ggagccgctc gacatgtggc gaggcgcctt cgcgctcact tatggagcgg agctgcgcgc
     1261 cgagcggcgc ggacgcgagg gctcggtcgt cgactccgat ccagagcgct accttcgctt
     1321 cagcaggccc gccatggcgg cggtgccggg cgacgggccg ctcgccaggg cagcgcggcg
     1381 gccctggtgg aagcggcgct tggttggcaa ggctctgacc gtcgtgcggc tgatgaaggc
     1441 gagcggcacc tatgccggcg ggatcgatta cctggcttgg aagatcaacc gtcacgccgg
     1501 gacgaagatc gaactgaagc cgtggcagcg gcgctggccg ctcgtcgccg ctatcggcct
     1561 gctgccgcgg ctgctgaggc ggggggccat tcgttaggcc ggcgggcgag cgccccggcg
     1621 acggcgc
//
LOCUS       DASVQZ010000237         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_49165, whole genome shotgun sequence.
ACCESSION   DASVQZ010000237 DASVQZ010000000
VERSION     DASVQZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49165"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VF547_06380"
     CDS             <1..294
                     /locus_tag="VF547_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX8262483.1"
                     /translation="DPKGKAALTRWRVVAVRDGRGIILFSPETGRTHQIRVHAAEGLG
                     LPIAGDPVYGGSSGPMLLHALSLRVERGAKPPIEATAPLPPTFVAAGFSDVLG"
     gene            281..694
                     /gene="arfB"
                     /locus_tag="VF547_06385"
     CDS             281..694
                     /gene="arfB"
                     /locus_tag="VF547_06385"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX8262484.1"
                     /translation="MSSAEPFTIPEEALDEKFLASTGPGGQNVNKVATACQLRCDVFR
                     LGLSPPVYARLKRLAGSRMTGDGEIIITARTHRTQEANREEARARLADLVAKAHVAPR
                     KRVKTTPSRAAKARRVDEKKGRGAVKAARGKVRLD"
     gene            complement(830..2248)
                     /locus_tag="VF547_06390"
     CDS             complement(830..2248)
                     /locus_tag="VF547_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8262485.1"
                     /translation="MSRIARTIAIAVSAACTCAWPAGGRERRSDAIAFVHAAVVPMDR
                     ERVLADQTVVVVGGKITAVGPASRVAVPRGAVRIDARGRYLLPALSDMHVHVEGESWN
                     ALLSPEAKAASKDTPFEDFLFPYVANGVTTVQVLSGTHELLPVRGRIAEGAMLAPRLV
                     LARMIDGPGKAWPPPLADWVATAREARDATLKAKADGYDKMKVYSFLNKESYDAVVAT
                     AREQRMGVVGHIPYALSVEYVVDAGQKMIAHTEEVAKHAHGDYSPERVSYFAGRIADG
                     GVWLTPTLVTTRRILDEFSDPDGLFSRPEAVYAAHPMQKDVWSFIANMYKGIPPADRA
                     KLRDDFEKFQRPFTKVFHDKGGQLMTGTDSLMPRLVPGFTVHQELQEFVAVGMTPFQA
                     LRTSTTIPYEYLGESKEAGTIEVGKRTDLLLLDANPLEEVSAASKIAGVMMRGRWIGR
                     EEIDRRMREIARKAAVAGAPAR"
     gene            complement(2465..2920)
                     /locus_tag="VF547_06395"
     CDS             complement(2465..2920)
                     /locus_tag="VF547_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde-activating protein"
                     /protein_id="HEX8262486.1"
                     /translation="MREEAALNLSCHCGQLRVEIAKRPDYVHECNCTLCRKAGARWGY
                     FHPSEVSVDGETAGYCREDKDDPAACIRFCAKCGVTTHFVLTESAAARHGNVVTGVNM
                     RLADEDDLSGVELRYPDGRAWSGQGGFGYVREPRILGRRAAPAGPPERS"
     gene            complement(2913..3659)
                     /locus_tag="VF547_06400"
     CDS             complement(2913..3659)
                     /locus_tag="VF547_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262487.1"
                     /translation="MWNQFRAIAARIGLSREQVEEVERGGVPTLVPDPLNSDIQYRVR
                     PDPDWTERMSDAWHGTLVNSRRESAGTLAVPDGRLRMADLISPERGVTAEVAPGEYEV
                     VLTIAHVGSEETYDRVDYVSHAFALLCGDEEVAAIEPLTDEDGTELGVEARSTLAFAT
                     AGLIERLAARQPGGIWRLTDLPGMTATAEGSEGKCVRLVADDGSGALIDVRAGHGRED
                     YPLFRIADPDGRTIGVLVDFFVDNRPHEDA"
     gene            3787..4308
                     /locus_tag="VF547_06405"
     CDS             3787..4308
                     /locus_tag="VF547_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8262488.1"
                     /translation="MYDFRIDAADKAEAWRQIVEAADSLTAGEPDAIANMANVAALLF
                     EALPELNWAGFYRNVGGELVLGPFQGRAACIRIPFGKGVCGAAAAGLKPQLVDDVEAF
                     PGHIACDSASRSELVVPLVEAGELIGVIDLDSPRLGRFDAEDLAGCLELARLLGPRLG
                     AGSKAPLQALKMS"
     gene            4360..5256
                     /locus_tag="VF547_06410"
     CDS             4360..5256
                     /locus_tag="VF547_06410"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEX8262489.1"
                     /translation="MSKLWLIGLAASAAWTPAAMAQGAPPAPPAPPMAHGAHAPGHGM
                     MHGGGHVIPPGLPMKGFHRFGPGHVVPPQFRGGQFIVHDWRMFGFPAPMPGGHWIRFH
                     DDALLIDQAGRVVDSRYGWDWDRRGDRMAHDDGRYDDDRDYDGDDDDRDDDDLGDYDR
                     GDHGGDEWDDEMPAGAHHGDRDRVVRIFRHDSRPKGHCRRRCAPVRHIPLPPHPGYGY
                     GYGYGYGYGYGAGCGCGGVVITETTTTTAPVVETRTYYETVTEHRAAPRRHYSKPVRR
                     YVKPVVRRSVKAAPAPRPGERG"
BASE COUNT          798 a         1856 c         1849 g          790 t
ORIGIN      
        1 gacccgaagg gcaaggcggc gttgacccgc tggcgcgtcg tcgcggtccg cgacggccga
       61 ggaataatcc tgttctcccc ggaaaccggc cgcacccacc agatccgggt ccatgccgcc
      121 gagggcctcg gcctgccgat cgccggcgac ccggtctatg ggggctcaag cgggcccatg
      181 ctgctccacg ccttgtcgct tcgggtcgag cgcggcgcca agcccccgat cgaggcgacg
      241 gctcccctcc ccccgacctt cgtcgcggcc ggcttcagcg atgtcctcgg ctgagccctt
      301 tacgatcccg gaggaggcgc tggacgagaa gttcctcgcc tcgaccggtc ccggcgggca
      361 gaacgtcaac aaggtcgcga ccgcctgcca gctgcgctgc gacgtcttca ggctcggcct
      421 ctcgcccccc gtctacgcgc gcctgaagcg gcttgccggc tcgcggatga cgggcgacgg
      481 cgagataatc atcaccgccc gaacgcaccg gacccaggag gccaaccgcg aggaagcgcg
      541 ggcgaggctg gccgacctgg tcgccaaggc ccacgtcgcc ccgcgcaagc gggtgaagac
      601 cacgccgagc cgagccgcga aggcccggcg ggtggacgag aagaaggggc gcggcgcggt
      661 gaaggcggcg cggggcaagg tgcggctgga ctagcggggg gctcgggtcg aagagcgggc
      721 gctccactac ccttgatgaa gcaggcgcga tccgcccggc ggccacctgc gatgcatcgt
      781 cactctttgg agtcttgcgc tgacccggga cccggcggtg ccgcctgcat caccgggcgg
      841 gggcgccggc aacggcggcc ttcctggcga tctcgcgcat cctccggtcg atctcctcgc
      901 gcccgatcca ccggccccgc atcatcaccc cggcgatctt cgacgcggcc gagacctcct
      961 cgagggggtt ggcgtcgagg agcagcaggt ccgtccgctt gcccacttcg atggttcccg
     1021 cctccttcga ttcgccgaga tactcgtagg ggattgtcgt cgaggtccgg agggcctgga
     1081 agggcgtcat cccgaccgcg acgaattcct ggagctcctg gtggacggtg aagccgggga
     1141 cgaggcgcgg catgagcgaa tccgtgccgg tcatcagctg gccgcccttg tcgtggaaga
     1201 ccttggtgaa cggcctctgg aacttctcga aatcgtcgcg gagcttcgcg cggtccgccg
     1261 gcgggatccc cttgtacata ttggcgatga acgaccagac gtccttctgc atcggatgcg
     1321 cggcgtagac ggcttccggg cgcgagaaga gcccgtcggg atcggagaat tcgtcgagga
     1381 tgcgccgggt ggtgaccagg gtcggagtca gccacacgcc tccgtccgcg atcctgccgg
     1441 cgaagtagga gacgcgttcg ggcgaatagt cgccgtgcgc atgcttggcc acctcctcgg
     1501 tgtgcgcgat catcttctgg ccggcatcga ccacatattc gaccgaaagg gcatagggga
     1561 tgtggccgac cacacccatg cgctgctccc tggccgtggc gacgacggcg tcgtaggatt
     1621 ccttgttcag gaacgagtag accttcatct tgtcgtagcc gtcggccttc gccttgaggg
     1681 tggcgtcgcg ggcttcacgg gcggtggcga cccagtcggc cagcggcggc ggccaggcct
     1741 tgcccggacc gtcgatcatc ctggccagga ccagcctcgg cgccagcatg gcgccctccg
     1801 cgatccggcc gcgcaccgga agcagctcat gggttcccga caggacctgg accgtcgtca
     1861 ccccgttcgc cacatagggg aagaggaagt cctcgaacgg agtgtccttg ctggcggctt
     1921 tcgcctccgg gctcagcagg gcgttccagg attcgccctc cacatggaca tgcatgtcgc
     1981 tgagcgcggg cagcagatag cggccgcggg cgtcgatcct gacggcgccg cggggcaccg
     2041 cgaccctcga cgcgggaccg acggcggtga tcttcccgcc gaccaccacc acggtctgat
     2101 cggcgaggac gcgttcgcgg tccatcggca cgaccgcggc gtgaacgaac gcgattgcgt
     2161 ccgatcgcct ctcccggccg ccggccggcc aggcgcaggt gcaggcggcc gaaaccgcaa
     2221 tggcgatggt gcgcgcgatc cgtgacatgg ctcgtccctt tcgaagctgg ccggccaggg
     2281 cttgcccggc gggccggcgg cgccaagggg cttttgcgcg aacggtgcat cggcttcgcg
     2341 aacggcgccg aggcgcgggc aggcccggcc gtggcgcaat gtccgcaccg gatcgattac
     2401 cgcgggaacc ggacggtccg cctccggccg gaacgccctc ccctccgatg cgccattccg
     2461 tcgactagga gcgttccggc ggcccggcag gggcggctcg gcggccgagg atgcgcggct
     2521 cgcgcacgta gccgaagccg ccctgcccgg accaggcgcg gccgtcgggg tagcgcagct
     2581 ccacaccgga gagatcgtcc tcgtcggcca ggcgcatgtt gacgcccgtg acgacgttgc
     2641 cgtgcctggc cgccgcgctt tcggtcagga cgaagtgggt cgttacgccg cacttcgcgc
     2701 agaaccggat acaggcagcc ggatcgtcct tgtcctcccg gcaatatccc gccgtttcgc
     2761 cgtcgacgct cacctcggag ggatgaaaat agccccagcg ggcgccagcc ttgcggcaga
     2821 gcgtgcagtt gcactcgtgg acataatccg ggcgcttcgc gatctcgacg cggagctggc
     2881 cgcagtggca ggacaggttc agcgcggcct cttcacgcat cttcatgcgg ccggttgtcg
     2941 acgaagaagt cgaccagcac gccgatcgtc cggccgtccg ggtcggcgat cctgaacagg
     3001 ggataatcct cgcggccgtg gcccgcccgg acgtcgatca gggcgccgct cccgtcgtcg
     3061 gcgacgaggc gcacgcactt gccctccgaa ccctccgccg tcgccgtcat gcccggcaga
     3121 tccgtcagcc tccaaatgcc tccgggctgg cgagcggcga gacgctcgat cagcccggcc
     3181 gtcgcgaacg cgagggtcga gcgcgcctcg actccgagct cggttccgtc ctcgtccgtc
     3241 agtggctcga tcgcggcgac ctcctcgtcg ccgcacagca aggcgaaggc atgggagaca
     3301 tagtcgacac gatcgtaggt ttcctccgag ccgacatggg cgatggtgag gacgacctca
     3361 tattcccccg gcgcgacctc ggcggtgacc ccccgttcgg gcgagatcag gtctgccatc
     3421 cgaagccgcc cgtcgggaac ggccagggtc ccggccgatt cccgccggct gttgacgagg
     3481 gtcccgtgcc aggcgtcgct catccgctcg gtccagtcgg ggtcggggcg aacgcggtac
     3541 tggatatcgg aattgagcgg gtcgggaacg agggtcggaa cgcccccgcg ctcgacctcc
     3601 tccacctgtt cgcgactcaa gccgatgcgg gccgcgatcg ccctgaactg gttccacatt
     3661 tgcgaattct ccgaaggtca aacgcggccg gccctcggat gctcccttga ccgaaccgcg
     3721 ccgaaggagc ggaaaaggcc ctcgcgaggc tggcggaatc gatcgccagg gcccacatcg
     3781 cgccccatgt acgacttcag gatcgacgcg gccgacaagg ccgaggcctg gcgccagatc
     3841 gtcgaggcgg ccgactcgct caccgccggc gagcccgacg cgatcgccaa catggccaat
     3901 gttgccgctt tgctgttcga ggcgctgccg gagctgaact gggcgggctt ctaccgcaat
     3961 gtcggcgggg agctggtcct gggaccgttc caggggcggg cggcgtgcat ccggatcccg
     4021 ttcggcaagg gggtctgcgg cgcggcggcc gccggcctca agccgcagct ggtggacgac
     4081 gtcgaggcct ttcccggaca tatcgcctgc gacagcgcgt cgcgttccga gctggtcgtg
     4141 ccgctggtgg aggccggcga gctgatcgga gtgatcgacc tcgacagccc gcggctcggc
     4201 cgcttcgacg ccgaggacct ggccggctgt ctcgaattgg cacggctgct cggaccacgc
     4261 ctgggcgccg gtagcaaagc ccccctccaa gcgttaaaaa tgtcttaacg agccccgact
     4321 ccgagccggg gccgaggcac tcgcgcacag gggacgaaga tgagcaagct ctggttgatc
     4381 ggcctggcgg cgtcggccgc ctggactccg gcggcgatgg cgcagggcgc gccgccggcc
     4441 ccgccggccc cgcccatggc ccatggcgcc cacgcgcccg gtcacgggat gatgcacggc
     4501 ggcggccacg tcattccgcc cggcctgccg atgaagggct tccaccgctt cgggcccggc
     4561 catgtcgtcc cgccccagtt ccgcggcggg cagttcatcg tccacgactg gcggatgttc
     4621 ggcttcccgg cgccgatgcc cggcggccac tggatccgat tccacgacga cgccttgctg
     4681 atcgaccagg cgggacgggt ggtcgacagc cgctacggct gggactggga tcgccgcggc
     4741 gaccgcatgg cccatgacga cgggcgctac gacgacgatc gcgactatga cggcgacgat
     4801 gacgaccgcg acgatgacga cctcggcgat tacgaccgcg gcgatcatgg cggcgacgaa
     4861 tgggacgacg agatgccggc cggagcgcat catggcgatc gcgaccgggt ggtgcggatc
     4921 ttccggcacg actcgcggcc gaaaggccat tgccggcgcc gctgcgcgcc ggtgcgccac
     4981 atcccgctgc cgccgcatcc cggctatggc tacggctatg gctatggcta cggatatgga
     5041 tatggggccg ggtgcggctg cggcggagtg gtgatcaccg agaccacgac cacgaccgcc
     5101 cctgtggtcg agacccggac ctattacgag acggtgaccg agcaccgggc ggccccgcgc
     5161 cgccactatt ccaagcccgt gcggcgctac gtcaagccgg tcgtccgccg ctcggtgaag
     5221 gcggcgcccg cgccgcggcc gggcgagcgg ggctagctca atcggtccag atcctcgtcg
     5281 ccgaggcggc cgg
//
LOCUS       DASVQZ010000238         8727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_49441, whole genome shotgun sequence.
ACCESSION   DASVQZ010000238 DASVQZ010000000
VERSION     DASVQZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49441"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..2870)
                     /locus_tag="VF547_06415"
     CDS             complement(18..2870)
                     /locus_tag="VF547_06415"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX8262490.1"
                     /translation="MTEDGRLLDLDSIGSEREQGPSWARAHWPIAELDELNAGLDPTL
                     MTIEKVAAKAKEAAAAAGGSTADVERAANDSICAMMLIRTYRVRGHLAANLDPLGLAK
                     RDMPEDLKPEYHGFSGAALDRPVWLGGAIGLQQATVREVVGILRRNYCGNVGVEYMHI
                     NDVEERRFLQERMEGPEAAIRFTPEGKRSILAKVIQAEQWEKFLARKYVGTKRFGLDG
                     GESMVPALEAVIKYGGADRVGSIVLGMAHRGRLNVLANVMGKPYRAIFSEFAGGATNP
                     EDVGGSGDVKYHLGTSSDREFDGIKVHLSLVPNPSHLEAVDPVVLGKARAQQVALGDV
                     DGDRVLPVLLHGDAAFAGQGIVAECLGFSGIPGYSTGGCLHYIINNQVGFTTSPQFGR
                     SSPYPSDVAKAIQAPILHVNGDDPEAVTFCCKLAIEYRQRFNRDIVIDMWCYRRFGHN
                     EGDEPSFTQPLMYAKIREHPPVSKLYADRLVNEGVIAPEWVDKEIADFTALLETEFEA
                     AKSYLPNKADWFEGAWAGLNRPQEAITERRNVATGVSEAVAREVGEILTTVPGDLAVH
                     KTLARIVEARREMFASGEGFDWATAEALAFGTLLREGHPVRLSGQDSGRGTFSQRHAV
                     WVDQKDGRKYIPLTRVGPARFEVRDSPLSEFGVMGFEYGYSLADPQTLVLWEAQFGDF
                     ANGAQVMIDQFIAAGEAKWLRASGLVLLLPHGYEGQGPEHSSARPERFLQLCAEDNMQ
                     VANCTTPANYFHILRRQMNRDFRKPLVLMTPKSLLRHKQAVSSLADFTGDSHFKRILS
                     DPNAPADEAVKRLVLCTGKLAYELLDARDQAGDRNTSILRIEQLYPFPGEALLARLKR
                     MTGLEEVVWAQEEPQNQGYWAHVEPRLERRLGEAGLKPKRPLYAGRPHAASPATGLAK
                     RHAAEQAALIAEALGHSPASLTTGSSDAAPPQLRAG"
     gene            complement(2981..3865)
                     /gene="sucD"
                     /locus_tag="VF547_06420"
     CDS             complement(2981..3865)
                     /gene="sucD"
                     /locus_tag="VF547_06420"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX8262491.1"
                     /translation="MSILVNSSTKVITQGMTGETGTFHTEQALAYGTKMVAGVTPGKG
                     GQTHLGLPVFDTVAEAVRETGADASVVYVPPPFAADSILEAIDAEVPLIVCITEGIPV
                     LDMVRVKRALSGSRSRLIGPNCPGVLTPDECKIGIMPGSIFRKGSVGVVSRSGTLTYE
                     AVFQTSNAGLGQSTAVGIGGDPVNGTNFVDVLELFLADPETRSIIMIGEIGGSAEEDA
                     AQFLADEARAGRGKPTVGFIAGRTAPPGRRMGHAGAIVSGGKGAAEDKIAAMEAAGIT
                     VSPSPSELGSTLAALLKG"
     gene            complement(3866..4426)
                     /locus_tag="VF547_06425"
     CDS             complement(3866..4426)
                     /locus_tag="VF547_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262492.1"
                     /translation="MLILAAFLFAAPPPAPERELLDAFNAACSRTGTDIAAMKADALA
                     TGWTEMADEAEPQVARLVRLGREAADADGKVDGASFRRKLGGRDVFLIVSRYEDRSRF
                     WGAGCRLYDFEATAPLDGPALENWMGKPPTHVEEPAPGLSKRLWEPGWRDGISLEISY
                     VAQGNPVGHQYGLSGNILVAQAIGGF"
     gene            complement(4458..4733)
                     /locus_tag="VF547_06430"
     CDS             complement(4458..4733)
                     /locus_tag="VF547_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262493.1"
                     /translation="MNRLGDNIAFGLIVGIPLALLCWGASTMLALVVGRPAAWVVAGA
                     VILAWPAYYVSTTHDDSQFTDGLGLLVLTAPAMMLGWIVALARVSRR"
     gene            complement(4738..5700)
                     /gene="mdh"
                     /locus_tag="VF547_06435"
     CDS             complement(4738..5700)
                     /gene="mdh"
                     /locus_tag="VF547_06435"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX8262494.1"
                     /translation="MARTKIALIGAGMIGGTLAHLAAIRELGDIVLVDVAEGIPQGKA
                     LDLAQAGPVEGYDATLTGTQDYADVAGADVCIVTAGVARKPGMSRDDLLGINLKVMKA
                     VGEGIKAHAPDSFVICVTNPLDAMVWALREFSGLPHERVVGMAGVLDSARFRHFLAEE
                     FRVSVEDVTAFVLGGHGDTMVPVPAYSAVAGIPVPDLIRMGWSTQERIDAIVQRTRSG
                     GGEIVALLKTGSAYYAPATSALMMAEAYLKDKKRVLPAAAYLQGQYGVDDLYVGVPVV
                     IGAGGVERIVEIELDEAAKANFQVSVDAVRELLDACRAIDESLK"
     gene            complement(5886..7298)
                     /locus_tag="VF547_06440"
     CDS             complement(5886..7298)
                     /locus_tag="VF547_06440"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8262495.1"
                     /translation="MRRLLFVAALGAAISSGASAQPASERWNAKAREILARAVEIPTV
                     QGRGKVPELARYLAGEYKAGGWAERDVQVVPYEGAPGDATAAFIARWPAARPSGKKPI
                     LLLAHMDVVEAKRDDWSLEPFVLTERDGYFYGRGTSDIKQGIAAVTTALLRLRAEGFR
                     PRRDIVVLFTGDEETAGRGAELGATEWRKWTDAEFALNSDGGGGGFAKDGRPLGFSLQ
                     TAEKTYSMYSFTVRNRGGHSSKPRGDNAIYKLAEALRRLEAYRFTPILNETTRAYFSI
                     REKGEKGPLGDAMRAWLADPNDGKAADLIEADEGEVGLTRTRCVATRLEAGHADNALP
                     QLARATVNCRIMPGTEPAAIRDELERMVGDPEVKVERTDDQKMSLASPLRPDVVAAFT
                     AAVHARHPGAPIMPEMSTGASDARPFRVAGIPVYGVDGSWGVVPDDLRAHGRDERLPV
                     KALYDDVDHWVGMLRTLAGK"
     gene            complement(7379..>8727)
                     /locus_tag="VF547_06445"
     CDS             complement(7379..>8727)
                     /locus_tag="VF547_06445"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8262496.1"
                     /translation="LQAKLKVAAVDDPLEREADRVADRLVGASAAPAAVSRAGAGDVR
                     RAEAGAGAAAGPGAEAALGGLGAGVPLGADERSFFEPRLGRDLSAIRIHTGGAADKAS
                     KAIAARAFTRGTDIAFADGEYQPGTPGGRHLLAHELAHTLQAGGGGAIRRKCAADPPD
                     CAEDKLKLAQAKVAGNTTYARFIYKAATSKCYRPTDIADSIRQVEPGLFSGSLAKYVE
                     TIVQPLNKTDPERIHAGDCFAFPFGWKDPNIGDVDAELAKLKTSAGAAEKNHIIATLY
                     AEQSAAGMDEQRKYIFYAMLLRIQHHNWGPNFQSVVTTGTFHATDPQTDTYKNNYVPA
                     KQYLEGAKPTKPVNTAVIDNLKAIVDGASAAGIPANAGPYYFHWSSETTTAESAYQAH
                     LKAKVAPDVAEKKAAYKQAKDRLGASLEGITEAQGWLHKVPGPDPASRFGAMYIYR"
BASE COUNT         1285 a         2982 c         3016 g         1444 t
ORIGIN      
        1 gtggccatct cgttccttta tcccgcacga agctgcggcg gcgcggcgtc gctgctgccg
       61 gtagtgagcg aggccggact atggccgagc gcctcggcga tcaaggccgc ctgctcggcc
      121 gcgtgccgct tggccagccc cgtcgccggc gaggcggcgt gcgggcggcc cgcatagagg
      181 ggccgtttcg gcttgaggcc ggcctcgccc agccggcgtt cgagccgcgg ctcgacatgc
      241 gcccaatagc cctggttctg cggttcctcc tgtgcccaga ccacctcctc gaggccggtc
      301 atccgcttca ggcgcgccag cagcgcctcg ccggggaagg gatagagctg ctcgatccgg
      361 aggatgctgg tgttgcggtc gccggcctgg tcgcgcgcgt cgagcagctc gtaggcgagc
      421 ttgccggtgc acaggaccag ccgcttcaca gcctcgtcgg cgggcgcgtt gggatcggag
      481 agtatccgct tgaaatggct gtcgccggtg aagtcggcaa ggctcgagac cgcctgcttg
      541 tggcggagca gcgacttggg ggtcatcagc accagcggct tgcggaagtc gcggttcatc
      601 tggcgcctga ggatatggaa ataattggcc ggcgtcgtgc agttggccac ctgcatattg
      661 tcctcggcgc agagctgcag gaagcgttcc ggccgagccg agctgtgctc gggcccctgg
      721 ccctcgtaac cgtgcggaag cagcagcacg aggccgcttg cccgaagcca tttcgcctcg
      781 ccggcggcga tgaactgatc gatcatgacc tgggcgccgt tggcgaaatc gccgaactgg
      841 gcttcccaca gcaccagcgt ctggggatcg gcgagcgaat agccatattc gaagcccatc
      901 accccgaact cggagagcgg gctgtcgcgc acctcgaagc gggccgggcc cacccgggtc
      961 aacgggatgt atttgcggcc gtccttctgg tcgacccaca ccgcgtggcg ctggctgaac
     1021 gtgccccgtc ccgaatcctg gcccgacagg cgcaccggat ggccctcgcg aagcagcgtt
     1081 ccgaaggcga gcgcctcggc ggtcgcccag tcgaagccct cgccgctcgc gaacatctcg
     1141 cggcgggcct cgacgatccg cgccagcgtc ttgtggacgg cgaggtcgcc gggaacggtg
     1201 gtgaggatct cgcccacctc gcgcgccacc gcttccgaga ccccggtggc aacgttgcgg
     1261 cgctcggtga tcgcctcctg cggtcggttg agcccggccc aggcgccctc gaaccagtcg
     1321 gccttgttgg gcagatagga tttcgccgcc tcgaactcgg tttcgagcag ggccgtgaag
     1381 tcggcgattt ccttgtcgac ccactcgggc gcgatcacgc cctcgttcac cagacggtcg
     1441 gcatagagct tcgagaccgg cgggtgttcc cggatcttcg cgtacatgag cggttgggtg
     1501 aagctcggct cgtcgccctc gttatggccg aagcggcggt agcaccacat gtcgatgacg
     1561 atgtcgcggt tgaagcgctg gcgatattcg atggcgagct tgcagcagaa ggtcaccgcc
     1621 tcgggatcgt cgccgttgac gtggaggatc ggcgcctgga tcgccttggc gacgtccgac
     1681 ggatagggag aggaccggcc gaattgcggg ctggtcgtga agccgacctg gttgttgatg
     1741 atgtagtgga ggcagccgcc ggtcgagtag ccggggatgc ccgagaagcc gaggcattcg
     1801 gcgacgatgc cctggccggc gaaggcggcg tcgccgtgga gcagcaccgg cagcacccgg
     1861 tcgccgtcga cgtcgccaag cgccacctgc tgcgcccgcg ccttgccgag aacgaccgga
     1921 tcgaccgctt cgaggtggct cggattgggg acgagcgaca ggtgcacctt gatgccgtcg
     1981 aactcgcggt ccgacgaggt gccgagatgg tatttgacgt cgcccgagcc gccgacgtct
     2041 tcggggttgg tggcgccgcc ggcgaattcc gagaagatcg cgcgataggg cttgcccatc
     2101 acgttggcga ggacgttgag tcggccgcga tgggccattc cgagcacgat cgatccgacc
     2161 cggtcggcgc cgccatattt gatcaccgcc tccagcgccg gcaccatgga ctcgccgccg
     2221 tcgagcccga accgcttggt gccgacatat ttgcgggcga ggaacttctc ccactgctcg
     2281 gcctggatga ccttggcgag gatcgacctc ttgccctccg gcgtgaagcg gatggccgcc
     2341 tcgggtccct ccatccgctc ctgcaggaag cggcgctcct cgacgtcgtt gatgtgcata
     2401 tattcgacgc cgacattgcc gcaataattg cgcctcaata tcccgacgac ctcgcggacg
     2461 gtcgcctgct ggaggccgat cgccccgccg agccagaccg gccggtcgag cgccgcgccg
     2521 gagaagccgt gatattcggg cttgaggtcc tcgggcatgt cgcgcttggc gaggccgagc
     2581 gggtcgagat tggcggcgag gtggccgcgc accctgtagg tgcggatcag catcatcgcg
     2641 cagatcgaat cgttggcggc ccgctccacg tcggcggtcg acccgcctgc ggccgcggcg
     2701 gcttccttcg ccttcgccgc gaccttctcg atcgtcatca gggtcgggtc gagacccgca
     2761 ttgagctcat cgagctcggc gatcggccag tgggcgcgcg cccagctcgg tccctgctcg
     2821 cgttcgctgc cgatgctgtc caagtccaaa agccttccgt cttccgtcat gccagcgcag
     2881 gctggcatcc aagccgactt cgagcctcac gccggagtcc gcttggaccc cagccttcgc
     2941 tggggtgacg gatcaatgcg tgaagcacgc agtctatccc tcagcccttc aaaagcgcgg
     3001 caagggtcga tcccagctca gatgggctcg gcgacaccgt aattccagcc gcctccatcg
     3061 ccgcgatctt gtcctcggcg gcgcccttgc cgccggagac gatcgcgccc gcatggccca
     3121 tgcggcggcc cggaggcgcg gtccggccgg cgatgaagcc gacggtcggc ttgccgcgcc
     3181 cggcccgggc ctcgtcggcc aggaactggg cggcgtcctc ctcggccgag ccgccgatct
     3241 cgccgatcat gatgatcgac cgggtttccg ggtcggcgag gaacagctcg aggacgtcga
     3301 cgaaattggt gccgttgacc ggatccccgc cgatcccgac cgccgtgctc tgcccgaggc
     3361 cggcgttgct ggtctggaac accgcctcgt aggtgagggt gcccgagcgg ctgacgactc
     3421 cgaccgagcc cttgcggaag atgctgcccg gcatgattcc gatcttgcac tcgtcgggcg
     3481 tcagcacgcc cgggcaattg ggcccgatca gcctcgacct cgagcccgac agcgcccgct
     3541 tcacccggac catgtcgagc accggtatgc cttcggtgat gcacacgatc agcggcacct
     3601 cggcgtcgat cgcctcgagg atcgaatcgg cggcgaaggg cggcggaacg tagacgacgc
     3661 tggcgtcggc gccggtctcc cggacggcct ccgccaccgt gtcgaagacg ggcaggccga
     3721 gatgggtctg gccgcccttc ccgggggtga ctccggcgac catcttcgtc ccgtaggcga
     3781 gcgcctgctc ggtatggaag gtgccggtct ccccggtcat gccctgggtg atcaccttgg
     3841 tcgacgagtt gacgaggatc gacattcaaa agcctccgat ggcctgggcg acgaggatgt
     3901 tgccgctgag gccgtattgg tgcccgacgg gattgccctg ggcgacgtac gagatttcga
     3961 gcgagatgcc gtcgcgccag ccgggctccc agaggcgctt cgacagcccc ggcgccggct
     4021 cctcgacatg ggtcggcggc ttgcccatcc aattttccag cgccggcccg tcgagcggag
     4081 ccgtcgcctc gaaatcgtag agccggcagc cggcgcccca gaagcggctc ctgtcctcgt
     4141 agcgggagac gatcaggaag acgtcccgcc cccccagctt gcggcggaaa ctggcgccgt
     4201 cgaccttgcc gtcggcgtcg gccgcctcgc gcccgagccg gacgagccgg gcgacttgcg
     4261 gctcggcctc gtccgccatc tcggtccagc ccgtcgccag agcatcggcc ttcatcgccg
     4321 cgatgtcggt gccggtgcgc gagcaggccg cgttgaacgc gtcgagcagc tcgcgctcgg
     4381 gcgccggcgg cggcgccgcg aagagaaaag ccgccaggat cagcatcgtt cgggaccagg
     4441 catgggcgct ccggagatca ccgacgcgat acgcgggcca gcgccacgat ccaacccagc
     4501 atcatggccg gcgcggtgag aacgagcagg cccaggccgt ccgtgaactg gctgtcgtcg
     4561 tgagtcgtgg agacataata agccggccag gcgaggatca ccgctccggc gacgacccag
     4621 gcggcggggc gcccgacgac gagagcgagc atcgtggagg cgccccagca cagcagcgcc
     4681 agcggaatgc cgacgattag cccgaaggca atgttgtcgc cgagccggtt cacgatccta
     4741 cttcaggctt tcgtcgatcg ccctgcaggc gtcgagcagc tccctcaccg cgtcgacgga
     4801 gacctggaag ttcgccttgg ccgcctcgtc cagctcgatc tcgacgatcc gctcgacgcc
     4861 gccggcgccg atcacaaccg ggactccgac atagaggtca tcgacgccat attggccctg
     4921 cagataggcg gccgcgggca ggacccgctt cttgtccttc agataggcct cggccatcat
     4981 cagcgcgctg gtcgccggcg cgtaataggc cgagccggtc ttcagcaagg cgacgatctc
     5041 gccgccgccg gagcgggtgc gctggacgat ggcgtcgatc cgctcctgcg tcgaccagcc
     5101 catccggatc aggtcgggca cggggattcc cgccaccgcg gaataggcgg gcaccggcac
     5161 catcgtgtcg ccgtggccgc cgagcacgaa cgcggtgacg tcctcgaccg aaaccctgaa
     5221 ctcctctgcc aagaagtggc ggaatcgcgc ggaatccagc actcccgcca ttccgaccac
     5281 acgctcgtgc ggaagccccg aaaactcccg cagcgcccag accatcgcgt cgagcgggtt
     5341 ggtgacgcag atgacgaaac tgtcgggcgc gtgcgccttg atcccctcgc cgaccgcctt
     5401 catcaccttg aggttgatcc cgagcagatc gtcgcggctc atgcccggct tgcgcgcgac
     5461 tccggcggtg acgatgcaga cgtccgcgcc ggcgacgtcg gcataatcct gggtgccggt
     5521 cagcgtcgcg tcatagcctt cgaccggtcc ggcctgggcg aggtcgagcg ccttgccctg
     5581 cgggattccc tcggcgacgt cgacgaggac gatgtcgcca agctcgcgga tggcggcgag
     5641 gtgggcgagc gtgccgccga tcatcccggc accgatgagc gcgattttcg tgcgagccat
     5701 ggggctgctt cagcctttct cttctcttag cggggcggcc gcaacggccg gcggaatgtg
     5761 gcgtccgctt agacccgaag gcggaagcgg gcaaccggct tggaaggggg aaattcgagt
     5821 cgggcccgcc ctggaggaag gaagcggcgc cgggcggcgg ccgcgcggcc gtgcgacgac
     5881 gggccttatt tgccggccag cgtcctgagc atgccgaccc aatggtcgac gtcgtcgtag
     5941 agggccttga ccggcagccg ctcgtcgcgg ccgtgcgccc gcaggtcgtc gggaacgacg
     6001 ccccagctgc cgtcgactcc gtagaccggg atgccggcga cgcggaacgg acgggcgtcg
     6061 ctggcgccgg tgctcatctc cggcatgatc ggcgcgccgg gatggcgggc gtggaccgcg
     6121 gcggtgaagg cggcgacgac gtcgggccga agcggcgagg cgagcgacat cttctggtcg
     6181 tcggtgcgct cgaccttgac ttccgggtcg ccgaccatcc gttcgagctc gtcgcggatc
     6241 gccgccggct cggtgccggg catgatccgg caattgaccg tcgcacgggc gagctggggc
     6301 aaggcgttgt cggcatgacc ggcttcgagg cgggtggcga cgcagcgcgt gcgggtgagg
     6361 ccgacctcgc cctcgtcggc ctcgatgagg tcggcggcct tgccgtcgtt gggatcggcc
     6421 agccaggccc gcatcgcgtc tcccagaggt cccttctcgc ccttctcgcg gatcgagaaa
     6481 taggcgcgcg tcgtctcgtt gaggatcggc gtgaagcgat aggcctcgag ccgccgaagc
     6541 gcctccgcga gcttgtagat ggcgttgtcg cccctcggct tggagctgtg cccgccgcgg
     6601 ttgcgaaccg tgaagctgta catggaatag gtcttctcgg ccgtctgcag gctgaagccg
     6661 agcggccggc cgtccttggc gaagccgccg ccgccgccgt ccgagttgag cgcgaactcg
     6721 gcgtcggtcc acttgcgcca ctcggtggcg cccagctccg cgccgcggcc cgcggtctcc
     6781 tcgtcgccgg tgaacagaac gacgatgtcg cgcctcggcc tgaacccttc cgcccgcagc
     6841 ctgaggagag ccgtcgtcac cgcggcgatg ccctgcttga tgtcgctcgt cccgcgcccg
     6901 tagaaatagc cgtcgcgctc ggtgaggacg aagggctcga gcgaccagtc gtcgcgcttc
     6961 gcctcgacca cgtccatatg ggccagaagc aggatgggct tcttccccga cggccgcgcc
     7021 gccggccagc gcgcgatgaa ggccgcggtg gcgtcgcccg gcgcgccttc gtacgggacg
     7081 acctggacgt ccctctcggc ccagcccccc gccttgtact cgccggccag ataccgggcg
     7141 agctccggca ccttgccgcg gccctggacg gtcgggatct cgacggctct cgccagtatc
     7201 tcccgcgcct tggcgttcca gcgctcggac gcgggctggg cggaggcgcc cgatgagatt
     7261 gcggcgccca aagcggcgac aaacagcaag cggcgcaccc ggatcccctt ttctgcgaag
     7321 cggcgacgat ccggtcttcc ggcggcgagg tcaagcccgc tgcggccggc ccgggccgtc
     7381 atcggtagat gtacatcgct ccgaaccggc tcgcgggatc cgggcccggc accttgtgga
     7441 gccatccctg agcctcggta atgccttcca gcgacgcgcc gaggcgatcc ttcgcctgct
     7501 tgtacgccgc cttcttctcg gcgacgtccg gcgccacctt ggccttcaaa tgggcctggt
     7561 acgcgctttc ggcggtggtg gtctcgctcg accagtggaa ataataaggc ccggcattgg
     7621 ccggaatgcc ggccgccgaa gcgccgtcga cgatcgcctt gagattgtcg atcacggcgg
     7681 tgttgaccgg cttggtcggc ttggcgccct cgagatattg tttagccggg acatagttgt
     7741 tcttgtaggt gtcggtctgc gggtcggtgg cgtggaacgt gccggtggtg acgaccgact
     7801 gaaaattcgg cccccaattg tgatgctgga tccgaagcag catcgcgtag aaaatatatt
     7861 tcctctgctc gtccatccca gcggccgact gctcggcgta aagcgtggcg atgatgtgat
     7921 tcttctcggc cgccccggca gaggttttca gcttcgcgag ctcggcatcg acgtcgccga
     7981 tgttcggatc cttccagccg aacggaaagg cgaagcagtc gcccgcgtgg atccgctcgg
     8041 gatcggtctt gttgagcggc tggacgatgg tctcgacata tttcgccagc gacccggaga
     8101 agaggccggg ctcgacctgc cggatggagt cggcaatgtc ggtcggacgg tagcatttcg
     8161 acgtcgcggc cttgtagatg aaccgggcat aggtggtgtt gccggcgacc ttcgcttggg
     8221 cgagcttcag cttgtcctcg gcgcagtccg gcggatcagc ggcgcatttc ctgcggatgg
     8281 cgcccccgcc gccggcctga agcgtgtgcg cgagctcatg ggcgagcagg tgccggccgc
     8341 cgggcgttcc cggttgatac tcgccgtctg cgaaggcgat gtcggtgccg cgcgtgaagg
     8401 cccgtgcggc gatcgctttc gacgccttgt ccgccgcgcc gccggtatgg atcctgatcg
     8461 cggacaggtc ccgtcccagc cgcggttcga agaagctgcg ttcgtcggca ccgagcggaa
     8521 ccccggctcc gagcccgccc agcgcggctt ccgcgcccgg gcccgccgcc gcgccggcgc
     8581 ccgcctcggc ccgccggacg tcgcccgcgc cggcccggga gacggcggcg ggcgcggccg
     8641 aagcgcccac gagtcggtcg gcgacccggt cggcctcccg ctccagcggg tcgtcgacgg
     8701 ccgcgacctt cagcttcgcc tgcaaaa
//
LOCUS       DASVQZ010000239         7868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_626532, whole genome shotgun sequence.
ACCESSION   DASVQZ010000239 DASVQZ010000000
VERSION     DASVQZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626532"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF547_06450"
     CDS             complement(<1..546)
                     /locus_tag="VF547_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262497.1"
                     /translation="MLDTLDVLIGFTVVMLVVSMAVTMLTQMIASGIANLRGLCLRQG
                     LSRLLALMDQGLTLPDAKAITDHVLRNPLVGRARIPIPKLRTGYALGTVIHREELIRL
                     LLDFAVPGDAEKTNHSDLPGEQLLRNKFRASLKRNGIDDPEAVLRQVRNAVVELEKSN
                     PELSHNARVSIALLNFGASEFL"
     gene            731..1378
                     /locus_tag="VF547_06455"
     CDS             731..1378
                     /locus_tag="VF547_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8262498.1"
                     /translation="MIVYGASLSPFVRKVLAYGAEKGIELEHKPIGLGSDDPGFLEAS
                     PFRKIPGFRDGDFAISDSSAIIHYLEAKYPQPPLIPAEPEARARVIWFDEFADTILCA
                     CGAKMFFNRIVAPRFLGRAGDLETADKAEKEELPPILDYLERTIPEGGWLVGGGLTLA
                     DIAVATAFANFRHLGMPVDAGRYPSVAEFADRMLARPSFAGWVEREAAFLERTSA"
     gene            1382..1834
                     /locus_tag="VF547_06460"
     CDS             1382..1834
                     /locus_tag="VF547_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8262499.1"
                     /translation="MGRYLATVEWRADGGFRDGRYSRAHRWTFDGGAVVAASASPDVV
                     PPPLSDPAGVDPEEALVASVSSCHMLWFLHLARDAGLDVAAYRDQAQGTMARDERGRI
                     AMTRIVLRPEIEFAGEAPAAEALARLHHEAHERCFIANSLRTEIVVEG"
     gene            1913..2590
                     /locus_tag="VF547_06465"
     CDS             1913..2590
                     /locus_tag="VF547_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262500.1"
                     /translation="MRLLAALLALAAVPAAAQPAAGPPSAALEALRRHFPEDHRALAA
                     SLAGKAPEEARRLAYAGIDKFLRDRVEAVLAAPGPALVAIEARQGALLRALGRQDVKL
                     CAVVGDRGFFSPEALAGAPPSGLDDYGAALVEAAKAGSAAPASAPAAASREDFLAWLE
                     RVGRIEPDVPVKAMLTDRELRLASSPDHLCRGSAAMHEAAAAMPGEAGERLARTLLRS
                     VIGIPRH"
     gene            3506..4249
                     /gene="rlmB"
                     /locus_tag="VF547_06470"
     CDS             3506..4249
                     /gene="rlmB"
                     /locus_tag="VF547_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX8262501.1"
                     /translation="MRRKARTASKPENRPRFWGRHAVVAALANPDREVVRIWATREAA
                     AEVELPKSVPFTFADAADLGRLVPRDAPHQGLVIEVERLDDLLLADVLQDAPERRPLL
                     VLDQVTDPHNVGAILRSAAAFDALALVTQDRHAPPESGALAKAASGALETVPWVRVVN
                     LARALDEMAEAGFWRIGLTGEAHLTLAEAVGPARTALVLGAEGEGMRPNTQAHCDALA
                     RLPISGRIESLNVSNAAAVALYAVATHGG"
     gene            4263..4991
                     /locus_tag="VF547_06475"
     CDS             4263..4991
                     /locus_tag="VF547_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262502.1"
                     /translation="MRFAFVAAAALVLSSCILSPGKFVSTLHIAKDRSFTFTYAGEVI
                     LLDPAAAMQQGMQDGLAGAAPPAENGMGEAGEADVNMADVAGSAPAAPAAETAQQVAE
                     GKAIAAALSKEVGYRSVEYLGKNKFRVDYAMSGKLDRSFVYPINLDAKSVIPWVAVEV
                     RTDRTVRVKAMAFGDPDEGMGGAAKPDSPEKERNGTFTFTTDAELVMQNNEDGMAPGP
                     GTRVVWKVTPTSTTVPTAVVRFAN"
     gene            complement(5037..5360)
                     /locus_tag="VF547_06480"
     CDS             complement(5037..5360)
                     /locus_tag="VF547_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8262503.1"
                     /translation="MANLTIVGRDGSEKTIEGKAGWSVMENIRDAGFDELLALCGGSC
                     SCATCHVHVDEGWLAALPPMGSDEDDLLDTTDHRRPNSRLSCQIEFGPALDGLRVSIA
                     PEDGF"
     gene            5408..6031
                     /locus_tag="VF547_06485"
     CDS             5408..6031
                     /locus_tag="VF547_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX8262504.1"
                     /translation="MGIAAGTLKTSLDALAAREPRIAAALAAVGYPEPRISEPGYQTL
                     LRAIVGQQVSIKAAASLWAKLAAAVGDPPAPDALLAASDEVLRGAGLSRQKASYARSL
                     AEEVVSGRLDFARLPEDDEEAIAELVRVKGIGRWTAEVYLLFAEGRTDIWPAGDLAVQ
                     IETGRILGLEGKPTEKRVRELAEAWRPHRGAFAIFAWHHRRNSAAPV"
     gene            6704..>7868
                     /locus_tag="VF547_06490"
     CDS             6704..>7868
                     /locus_tag="VF547_06490"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX8262505.1"
                     /translation="MSTATLSLPKGGGTVQGLGADLQASPFRGTARLTIPIALPQGRG
                     ASPALSLLYESNGGNGPFGAGAAVALPSLVLSTATGVPTYTDADPVVFSEVGLLAEKG
                     DWTAGRWVPSERTVEDAGGTAWRVHEFLPRLQGTFPLIERWTRVDDSTSHWRTVSADN
                     VESRFGVSAQSRIADPERPWQVFEWLLEEVVDANGNRARYRYKAEDDQGARRRPGSGA
                     NRYVERILYGNHPDGETAAYAFEVVFDYGEYDLDDLDKPAADPYRPVRPWPAREDGFS
                     SYRSGFELRTNRLCRGILTFHRFPAELGAEPCLTAATRFDYTPSPYLSCLRGVTQHGY
                     RRAPDGSYRTEALPPVELGFAGFDPPPAPAFRRLEVEREADLPGYLAPGAYQPI"
BASE COUNT         1167 a         2718 c         2725 g         1258 t
ORIGIN      
        1 gaggaactcg ctcgcgccga aattgagcag ggcgatgctg acccgggcat tgtggctcag
       61 ctcgggattg gacttctcga gctcgacgac ggcgttgcgg acctggcgca ggaccgcttc
      121 gggatcgtcg atgccgttgc gcttgagcga ggcgcggaac ttgttgcgca gcaactgctc
      181 ccccggcagg tcgctatggt tggtcttctc ggcgtcgccg gggaccgcga aatcgagcag
      241 cagccggatc agctcctcac ggtgaatcac cgtgccgagc gcgtagccgg tgcgcagctt
      301 gggtatcggg atgcgggccc gcccgaccag cggattgcgg agcacatggt cggtgatggc
      361 cttggcatcg ggcagggtaa ggccctggtc catcagcgcc agcagcctgg acaggccctg
      421 cctcaggcac aggccgcgca gattggcgat gccggaggcg atcatctggg tgagcatcgt
      481 gaccgccatg ctcacgacca gcatgaccac ggtgaatccg atcaggacgt cgagcgtatc
      541 gagcatggcg cagccccctg cggcgataga cgaacaagcg actcggtgaa cgccacgcaa
      601 cccgccccgg caaagctatc ccgccccggc gcaatggcaa ccgattatgg aatagggctg
      661 acgagatttg ttgccgtcca cggcgaagcg ggataaggtt tcatatcgaa accagtcccg
      721 ggagcaggcc atgatcgtct atggagcatc tctgtcgccg ttcgtccgga aggtgctggc
      781 ctatggggcg gaaaagggga tcgagctcga gcacaagccg atcggcctcg gctcggacga
      841 tccgggcttc ctcgaggcga gcccgttccg caagatcccg ggcttccgcg acggcgattt
      901 cgccatctcc gattcctcgg ccatcatcca ctatctcgaa gccaaatatc cgcagcctcc
      961 gctgatcccg gccgagccgg aggcgcgggc cagggtgatc tggttcgacg aattcgccga
     1021 caccatcctg tgcgcgtgcg gagcgaagat gttcttcaac cggatcgtcg ccccgcgctt
     1081 cctcggccga gcgggcgacc tcgagacggc cgacaaggcc gagaaggagg aactgccgcc
     1141 gattctcgac tatctcgagc ggaccatccc cgagggcggc tggctggtcg gcggcggcct
     1201 caccctggcc gacatcgccg tcgcgacggc ctttgccaat ttccgccatc tcggaatgcc
     1261 ggtcgacgcc ggccgctatc caagcgtcgc cgagttcgcc gacaggatgc tggcccggcc
     1321 gagcttcgcc ggctgggtgg agcgcgaggc ggccttcctc gagcgcacgt cggcctgaat
     1381 cgtgggccgc tatctcgcga cggtggaatg gcgggcggac ggcggtttcc gcgacgggcg
     1441 ctacagccgc gcccatcgct ggaccttcga cggcggcgcg gtggttgcgg cctcggcctc
     1501 gcccgacgtc gtgccgccgc cgctgtcgga tccggccggg gtcgatccgg aggaggcgct
     1561 ggtcgcctcc gtctcctcct gccacatgct ctggttcctc catctcgccc gcgacgcggg
     1621 cctcgacgtc gccgcctatc gggaccaggc gcaagggacg atggcccggg acgagagggg
     1681 gcggatcgcc atgacccgga tcgtgcttcg gcccgagatc gagttcgccg gcgaggcgcc
     1741 cgccgccgag gctctggccc gcctccacca cgaagcgcat gagcgctgct tcatcgccaa
     1801 ttcgcttcgc accgagatcg tcgtcgaggg gtaggcgcgc ttgcccggcg gcttgcgccg
     1861 gttcggcatt gcgctactcg gaaaatcgaa tccgctgaga aagttgcccg ccatgcgtct
     1921 cctggccgcc cttcttgccc tcgccgcggt ccccgccgcc gcccagccgg cggccggtcc
     1981 gcccagtgcc gcgctggagg cgctgcgccg ccattttccg gaggaccacc gggccctggc
     2041 cgcgagcctc gccggcaagg ccccggaaga ggcccggcgc ctggcctatg ccggcatcga
     2101 caagttcctg cgcgaccgag tcgaggcggt gctggcggcg cccgggccgg cgctggtcgc
     2161 gatcgaggcg cgccagggtg cgctgctccg ggccctcggc cgccaggacg tgaagctctg
     2221 cgcggtcgtc ggcgaccgcg gcttcttcag ccccgaggcg ctggccgggg cgccgccttc
     2281 gggcctcgac gactatggag cggcgctggt cgaggccgcg aaggcgggat cggcggcgcc
     2341 cgcctcggcg ccggcggcgg cgagccggga agacttcctc gcctggctgg agcgggtcgg
     2401 gaggatcgag cccgacgttc ccgtcaaggc gatgctgacc gaccgcgagc tgcgcctagc
     2461 ctccagcccc gaccatctgt gccgcgggtc ggcggcgatg cacgaggcgg ccgcggcgat
     2521 gcccggcgag gcgggcgaac ggctcgcgcg caccctgctg cgctcggtga tcggaatccc
     2581 gaggcactga gccgccgccg cggcgcgggg ggcgacgtcg ccgggcgaat cggggattcg
     2641 atctcggctt cgccggaggc gagcggcggc cgcccggaat attcggcgtg catggcccaa
     2701 acgaaatgga ggccggcgtc gccgccggcc tccactgtcc ctaggttcgt tggtccaacc
     2761 cgtgaaggcg aaaccgcctt tcccagggag gttccggccg aggccttgcg gccccggtcg
     2821 gtccctcagg ttccttcacc gggcgaaccc ggatcagtcc cctgcgggtc ccgcgcctgg
     2881 ccctaaggct ggccacggtt ccctggcggg atccgaactc tccgccttgc ggcttcgaaa
     2941 ttcgggttcc ctatcggact ccgacgaagc gaagcttcct cggcgtcctt tcgcgaaacc
     3001 ttccgcccgg gggcttccgg tttcgccgga ccctcgaagg aaagtgggtt cggctttggc
     3061 ttgcgccgcc gcctcgcgct tcttccttcc ggttgcccgg gacctcgctt cgatcagctt
     3121 cagtccgaag acctgagccg ctctccgcgg ggccatggtt gggatcgtct ctccgtttcc
     3181 ggagggcctc gcccttccac gagccaataa gtggcagaga tcaccgagtc gcggcaaacg
     3241 ctatttgggg gattcggcct gtggataacg gggataacgg ggataacaca ggttaaactg
     3301 gtcaggggcc cgccacggcc cgcccgaagc tccgccgggc cacggtccgg gcgatgaaaa
     3361 gcttgccgaa tcggcgccgg caccgccgtt gcacgggcgg acgggaggcg ccaagccatg
     3421 gcggtttcgc gccggctcgt ttcgacggct aaccaacctt ggcggccggc ttgcaaggcg
     3481 tgccccgcgc cgtcatggac gcggcatgcg ccgcaaagcc cgaaccgcct ccaagcccga
     3541 gaaccgcccc cgcttctggg gtcgccacgc ggtcgtcgcg gcgctcgcca atccggaccg
     3601 cgaagtggtt cggatctggg cgacgcgcga ggcggcggcc gaggtcgagc tgcccaagag
     3661 cgtgcccttc accttcgccg acgccgccga cctcggccgc ctggtgccgc gggacgcgcc
     3721 acaccagggg ctggtcatcg aggtcgagcg gctcgacgac ctgctcctcg ccgacgtgct
     3781 ccaggacgcg cccgagaggc ggcccctgct ggtcctcgac caggtcaccg acccccacaa
     3841 tgtcggcgcc atcctgcgtt cggcggcggc cttcgacgcc ctggccctgg tcacccagga
     3901 tcgccacgcg ccgcccgaat cgggcgctct ggcgaaggcg gcgagcgggg cgctggagac
     3961 ggtgccctgg gtgcgcgtgg tcaacctcgc gcgagcgctg gacgagatgg ccgaagcggg
     4021 tttctggcgg atcggcctga ccggcgaggc ccacctcacc ctggccgaag cggtcggtcc
     4081 ggctcggacg gccctggtgc tgggcgccga aggggagggg atgcgcccta acacccaagc
     4141 gcattgcgac gccttggccc ggcttccgat cagcggccgg atcgagagcc tcaatgtttc
     4201 caacgccgcg gcggtcgcgc tatacgcggt cgcgacacat ggggggtaat catatgccgt
     4261 tgatgcgttt cgcgttcgtg gcggcggccg cgctggtgct ttcgtcctgc atcctgagcc
     4321 cggggaaatt cgtctccacg ctccacatcg ccaaggaccg gagcttcacc ttcacctatg
     4381 ccggcgaggt gatcctgctc gatcccgccg cggcgatgca gcagggcatg caggacgggc
     4441 tcgccggtgc ggcgccgccg gccgagaacg ggatggggga ggcgggcgag gccgacgtca
     4501 acatggccga cgtcgccggc tccgctcccg cagcccccgc cgccgaaacc gcgcagcagg
     4561 tcgccgaagg caaggcgatc gccgccgctc tgtcgaagga ggtcggctac cggtcggtcg
     4621 agtatctggg gaagaacaag ttccgggtcg attatgcgat gagcgggaag ctggaccgga
     4681 gcttcgtcta tccgatcaac ctcgacgcca agtcggtcat cccctgggtc gccgtcgagg
     4741 tcaggaccga ccgcaccgtg cgggtcaagg cgatggcctt cggcgacccg gacgagggca
     4801 tgggcggcgc cgccaagccc gacagcccgg agaaggagcg caacggcacc ttcaccttca
     4861 ccaccgacgc cgagctcgtc atgcagaaca acgaggacgg aatggcgccc ggcccgggca
     4921 cccgggtcgt gtggaaggtc accccgacca gcacaacggt gccgaccgcg gtggtccggt
     4981 tcgcgaacta gacggtccac cctctccgac cggcttcgcc ggcggggagg ggagggtcag
     5041 aatccgtcct cgggagcgat gctgacgcgc aggccgtcca gcgcgggccc gaactcgatc
     5101 tggcacgaca ggcggctgtt cggcctgcga tggtcggtcg tgtcgagcag gtcgtcctcg
     5161 tccgagccca tcggcggcaa ggcggcgagc cagccctcgt cgacatggac gtggcaggtg
     5221 gcgcagctgc agctgccgcc gcacagcgcc agcagctcgt cgaagccggc gtcgcggata
     5281 ttctccatca cgctccatcc ggccttgcct tcgatggtct tctccgagcc gtcgcggccg
     5341 acgatggtga ggttcgccat gggccgcgcc tatgctaggc gcgattgtcg aacacaaggg
     5401 agcaattgtg ggcatcgccg ccgggaccct gaagacgtcg ctcgacgcgc tggcggcgcg
     5461 cgagccgcgg attgccgccg ccctggccgc ggtcggctat ccggagccgc ggatcagcga
     5521 gcccggctac cagacgttgc tgcgcgccat cgtcggccag caggtctcga tcaaggcggc
     5581 ggcgtcgctg tgggcgaagc tcgccgccgc ggtcggcgat ccgcccgctc cggacgcctt
     5641 gctggccgcc tccgacgagg ttctgcgcgg ggccggcctg tcgcggcaga aggcctccta
     5701 cgcccgaagc ctcgccgagg aggtcgtgtc gggacggctc gatttcgccc ggctgcccga
     5761 ggacgacgag gaagcgatcg ccgagctggt ccgagtgaag ggcatcggcc gctggaccgc
     5821 cgaggtctat ctgctgttcg ccgagggccg gaccgacatc tggccggccg gagacctcgc
     5881 cgtccagatc gagaccggcc ggatcctggg gctggagggc aagccgaccg agaaacgcgt
     5941 gcgcgagctc gccgaagcct ggcgcccgca ccgcggcgcc ttcgccatct tcgcctggca
     6001 ccaccggcgg aactcggccg ctccggtttg agtccttgtc cttcgagacg gtcgcgcggg
     6061 tcgatgcgct tcgtcgcgaa tcgaaggtcg gcgcggtttg catccgggag ggcgctccgc
     6121 ccgcgtcggg tcgcgatttt gtcccgttcg gagctgggcc gaccgcccgg caaactcgaa
     6181 aatgcttctc cgccaatgat atcttataat agctccgaag tggacggaat gactgttcgg
     6241 cggggttgca ggacataggg cgtcgtgcga tattattggc acctagggcg tttctagtaa
     6301 tcacactttt cgagctatcc gggattgcat gcagcgccgt ctccgctgtc atggtcatta
     6361 tcggaacact atacccgact gtattcaggt ccaggttgct tgagtgcaag ctgatcctga
     6421 gtcgataggc tgatattcaa caagactatg tcttgttggc aatatcatat tgcttggaag
     6481 agcgtggttc gctggtccgg tttccagcgg ggcaggtctt gcacctgtcg cgacggagag
     6541 aatttcggag cagccgatag gccgtccttc gttggcggca ggaggaagcc cctgctgtcg
     6601 tccaagtgag cgtagtcgcc agtcatgcgc ggtaccggtc ggtgccgagc cgtctgtcgt
     6661 cgtcacctgg gcaatcgatc ggaccgcgca tgggggttca agcttgagta ccgcgacgct
     6721 ctccctcccc aagggcggcg ggaccgtgca gggcctcggt gcggacctcc aggcctcgcc
     6781 gttccggggc accgcgcgcc tcacgatccc gatcgcgctt ccccaaggcc gcggcgccag
     6841 cccggccttg tcgcttctct atgaatcgaa cggcggcaac ggcccgttcg gcgcgggagc
     6901 cgcagtcgcc ttgccctcgc tggtgctgtc gacggcaacc ggcgttccca cctacaccga
     6961 cgccgatccc gtcgtctttt ccgaggtcgg cctcctggct gaaaaggggg actggacggc
     7021 gggccgctgg gtgccgagcg agcggacggt ggaggacgca ggaggcacgg cctggcgcgt
     7081 ccacgagttc ctgcccaggc tgcaggggac cttcccgctg atcgagcgct ggacgcgggt
     7141 cgacgacagc accagccatt ggcggaccgt cagcgccgac aatgtcgagt cgcgcttcgg
     7201 tgtctccgcg caatcccgga tcgcggaccc ggaacgtccc tggcaggtgt tcgagtggct
     7261 gctcgaggaa gtggtcgacg ccaacggcaa tcgcgcgcgc taccggtaca aggcggagga
     7321 cgaccagggg gcgcggcggc gcccgggctc cggcgccaac cgctatgtcg agcgcattct
     7381 ctacggcaat catcccgacg gggagacggc cgcctacgcc ttcgaggtcg tgttcgacta
     7441 tggcgaatac gacctcgacg acctcgataa gccggccgcg gacccctacc ggccggtccg
     7501 gccctggccg gcccgcgaag acggattctc gagctaccgg tccggcttcg agctgcgcac
     7561 caaccggctg tgccgcggca tattgacctt ccatcgcttt ccggccgagc tcggcgccga
     7621 gccctgcctg accgcggcga cgcgcttcga ttacacgcct tcgccctatc tgtcgtgcct
     7681 gcggggggtc acccagcacg gctatcggcg cgcccccgac ggcagctatc ggaccgaggc
     7741 gctgccgccg gtcgagctcg gcttcgcggg cttcgacccc ccgcccgcgc cggccttccg
     7801 gcgcctcgag gtggaacggg aggccgacct gcccggctac ctcgccccgg gcgcctatca
     7861 gccgatcg
//
LOCUS       DASVQZ010000240         5652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_49569, whole genome shotgun sequence.
ACCESSION   DASVQZ010000240 DASVQZ010000000
VERSION     DASVQZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49569"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3334
                     /locus_tag="VF547_06495"
     CDS             <1..3334
                     /locus_tag="VF547_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262506.1"
                     /translation="ADCARSTRSAGRAFSRFVDGRGGALKRVDFKLVEAAPRAGRATA
                     ARPRPAPMSNADAAGAERDWLAGEEPGLAWLFPGPDHNPRAPIVRVAIKHRPGQTVKL
                     LAGGRPVDPVAFDGSRKNESGTVAVSLWRGIPIDGRQAELTAEVRNADGSLAETLRRT
                     VRYGASPMRAELLRDRSLLVADGVSRPVRHGLTGDFEVPAPYYPAVEADAQQARQLAG
                     LERARPFWRVEGEDGIAWIELEPTTASGTVSLRFHFRDGEAAREQRLEAWLDPGRRPW
                     TIVGLAEGTVGFNRLQGKVEDLGEEDEKDVTDGRVALYAKGRVRGKWLMTLAYDSDKK
                     EDETRFGGVIDPDAYYTVYADRSERRYDAASIRKLYLKLERPQFYALFGDYETGIDEP
                     ELARYVRAFNGVKAEYRSDRIAASAFAADSPNRHRHDEIQGNGLSGPYALGARTILAN
                     SERVWIEVRDRLRSDRIVERRLLTRNIDYDIDYAAGTLRFREPILSRSPALDPQFIVA
                     DYEVDGIASRTVNAGGRVSWRSRDEKVQVAATVLSDSNDQARTSLAGADVRYRPSEST
                     EIRAEVAVSDRNLKSGSAASAAGTATAWQVEAEHHGSKLDVLAYAREREAGFGVGQTG
                     GAEDGTRKFGVDARARLGEAWSLTGSAWHEDYLASDARRIAARVLVEYRTPALAARAG
                     LTFADDRLADGRRARSTLLQLGATKRLLDNKLELDGQTEIPLGNAESIDFPARHRISA
                     RYAVTSGIQLVGAYEIADGKTVDARTARVGFDVQPWAGARIAVSGNVQDIAEYGPRSF
                     AAFGLSQSLVLDKHWSVDFSVDANKTLGGIDPARVLNPLHPVASGGFIGGGAGTLTED
                     FTALTAGATYRAGRWSVTGRAEYRAGDQGDRYGLTAAALRQMGDGEAVGGAFSGFVAR
                     AANGAETRTVDLQLSWARRPAGSRWSWLEKLELREDRVAGAVAGQPRPIGIPLTISGD
                     ARSRRVVNSLSLNYSPGGGSEVSLFWGSRYVSERFGDQDVGGWSNVVGADFRFDLSDT
                     LDVGLAGSVRGGLGASSFAWSAGPSLGFTPFENGWVSVGWNIAGFHDRDFEEARYTRN
                     GPYVTMRFKFDQLTLQSLLGNR"
     gene            3334..5043
                     /locus_tag="VF547_06500"
     CDS             3334..5043
                     /locus_tag="VF547_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEX8262507.1"
                     /translation="MRLLRLLLFVLAAFGFGSAAQAQLTFQLRTGDPATNIQSMPIDS
                     NNCAAAGPRTMYVGGVITNTGLTTVNNIVATMSGVTGNFALGQAPGQTVGSLGPGQST
                     GVYWQVDYGCSVATITATVSVASSGAPVSGIVGLRTVSSISANAGGNVLSSTLGPGAV
                     VGQTIWFDASYDFGGTDIGDEFILQPAGNPSFDAVCFRLVGTEIRSSNIAPITVGTMN
                     RIYFRQTQKQSGNGYFASVRYYFEYQCAGASTTARPYATMTSGNALKYTGNYDGASSF
                     SITFPGATNPFTISKTQDTASAIAGNSATVKYTITVSNPSAYASRISQFVDTLPAGAK
                     FLALDSASSVTAANSSSVPAANATGTLTFTGRQDQSYLIAAGGTVSLIYTVQMPSTAG
                     SYTNSARAVFGTASTSTATATFNVVTPAPLKMEKSSQASSDPVNGTANPKLIPGGRAA
                     YTVTITNPNSFATTADSIVILDGTPANLSLFVSHVSGSSGGPIRFQNGSTPSGLTYTF
                     TSLASTTDDVDFSNDGGATWTYVPTPNAQGADPAVNRVRIRPKGAMAANSSFSLLFGY
                     VIR"
     gene            complement(5045..5129)
                     /locus_tag="VF547_06505"
     tRNA            complement(5045..5129)
                     /locus_tag="VF547_06505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5093..5095),aa:Leu,seq:caa)
     gene            complement(5298..>5652)
                     /locus_tag="VF547_06510"
     CDS             complement(5298..>5652)
                     /locus_tag="VF547_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8262508.1"
                     /translation="VQAVAAADLVLPPPSEGSAEVARRVGAARAIQTRRFEGEGIRTN
                     AEVDGDRLEAVATPDEPGRRLLAQAAEAMRLSARGFHRVMRVARTVADLAGSEEVRRI
                     HVAEALSYRRQAPRN"
BASE COUNT          876 a         2131 c         1868 g          777 t
ORIGIN      
        1 ggccgactgc gcgcgcagca cacgctccgc cgggcgcgcc ttctcgcgct tcgtcgacgg
       61 ccgcggcgga gccctcaagc gagtcgactt caagctggtc gaagccgctc ccagggccgg
      121 ccgcgcgacc gcggcgcggc cccggccggc cccgatgagc aatgccgacg ccgccggcgc
      181 ggagcgcgac tggctggccg gcgaggagcc gggcctggcc tggctgttcc ccggccccga
      241 ccacaatccg cgggcgccga tcgttcgggt cgcgatcaag caccggcccg gccagacggt
      301 gaagctgctc gccggcggcc ggccggtcga tcccgtcgcg ttcgacggca gccgcaagaa
      361 cgagtccggc accgtcgccg tcagcctgtg gcggggaatc ccgatcgacg ggcgccaggc
      421 cgagctgacc gccgaggtcc gcaacgccga cggcagcctc gccgagaccc tgcgccgaac
      481 cgtccgctac ggcgccagcc cgatgcgggc cgagctgctg cgcgaccgct cgctgctggt
      541 cgccgacgga gtcagccggc cggtccgcca cgggctgacc ggcgatttcg aggttcccgc
      601 gccttattat ccggccgtcg aggccgacgc ccagcaggcc cgtcagctcg ccgggctcga
      661 gcgcgcgcgc cccttctggc gggtcgaggg cgaggacggg atcgcctgga tcgagctcga
      721 gccgaccacc gcctcgggca ccgtcagcct gcgcttccat ttccgcgacg gcgaggccgc
      781 ccgcgagcag cggctcgagg cctggctcga tccgggccgc cggccctgga cgatcgtcgg
      841 cctcgccgag gggacggtcg gcttcaatcg gctgcagggc aaggtcgagg atctcggcga
      901 ggaggacgaa aaggatgtca cggacggccg cgtcgctttg tacgcgaagg gaagagttcg
      961 cggtaagtgg ctgatgacgc tcgcttacga cagcgacaag aaggaggacg agacccgctt
     1021 cggcggagtc atcgatcccg acgcctatta caccgtctac gccgaccgca gcgagcgccg
     1081 ctacgacgcg gcctcgatcc gcaagctcta tctgaagctc gagcggccgc aattctacgc
     1141 cctgttcggc gattacgaga ccgggatcga cgagcccgag ctcgcccgct acgtgcgcgc
     1201 cttcaacggc gtcaaagccg aataccggtc ggaccggatc gccgcgagcg ccttcgccgc
     1261 ggacagcccc aaccgccacc gccacgacga gatccagggc aacggcctca gcggccccta
     1321 cgcgctcggc gcccgcacca tcctcgccaa cagcgagcgc gtctggatcg aggtccgcga
     1381 ccggctccgg tccgaccgga tcgtcgagcg ccggctgctg acccggaaca tcgattacga
     1441 catcgactat gccgccggca ccctgcgctt ccgcgagccg atcctgagcc gaagccccgc
     1501 gctcgacccg cagttcatcg tcgccgacta cgaggtggac ggaatcgcca gccgaacggt
     1561 caacgccggc ggtcgggtga gctggcgcag ccgtgacgag aaggtccagg tcgccgcgac
     1621 cgtgctcagc gattccaacg accaggcccg gaccagcctc gccggcgccg acgtccgcta
     1681 ccgtccttcc gaatcgaccg agatccgcgc cgaggtggcg gtcagcgaca ggaatctgaa
     1741 aagcgggtcg gccgcctcgg cggcgggcac cgccaccgcc tggcaggtcg aggccgagca
     1801 ccacggctcg aagctcgacg tcctggctta cgcccgcgag cgcgaagccg gcttcggcgt
     1861 cggccagacc ggcggcgccg aggacggcac ccgcaagttc ggcgtcgacg cccgagcccg
     1921 cctcggcgag gcctggtcgc tgaccggcag cgcctggcac gaggactatc tcgccagcga
     1981 cgcccggcgc atcgccgccc gggtgctcgt cgaatatcgc acgccggccc tcgcggcgcg
     2041 cgccggcctc accttcgccg acgaccgcct cgccgacggg cgccgggcgc gctccaccct
     2101 gctccagctc ggcgccacca agcggctgct cgacaacaag ctcgagctgg acggccagac
     2161 cgagatcccg ctcggcaacg cggaaagcat cgactttccc gcccgccacc gcatcagcgc
     2221 ccgctacgcc gtgaccagcg gcatccagct ggtcggcgcc tatgagatcg ccgacggcaa
     2281 gaccgtcgac gcccgcaccg ctcgggtcgg cttcgacgtc cagccctggg cgggcgcccg
     2341 gatcgccgtc agcggcaacg tccaggacat cgccgaatac ggcccgcgca gcttcgccgc
     2401 cttcggcctg tcgcagtcgc tggtgctcga caagcactgg tcggtcgact tctcggtcga
     2461 cgccaacaag actctcggcg ggatcgaccc cgcccgggtg ctcaacccgc tgcacccggt
     2521 cgcgagcggc ggcttcatcg gcggcggggc cggcaccctg accgaggatt tcaccgctct
     2581 caccgccggc gccacctacc gcgccggccg gtggagcgtc accggccgcg ccgaatatcg
     2641 ggccggagac cagggcgacc gctacggcct caccgcggcg gcgctgcgcc agatgggcga
     2701 cggcgaggcg gtcggaggcg cgttcagcgg cttcgtcgcc cgggccgcga acggcgccga
     2761 gacccgcacc gtcgacctcc agctcagctg ggcccgccgc ccggccggga gccgctggtc
     2821 ctggctcgag aagctcgagc tgcgcgagga ccgggtggcc ggggccgtcg ccggccagcc
     2881 gcggccgatc ggaattccgc tgacgatcag cggcgacgcc cggtcgcggc gggtcgtcaa
     2941 ctcgctttcg ctcaactact cgccgggcgg gggctcggaa gtctccttgt tctggggctc
     3001 gcgctacgtc tccgaacggt tcggcgacca ggacgtcggc ggctggagca acgtcgtcgg
     3061 cgccgatttc cgattcgacc tcagcgacac gctcgacgtc ggcctcgccg gcagcgtccg
     3121 cggcgggctc ggcgcctcct ccttcgcctg gtcggcaggc ccaagcctcg gcttcacccc
     3181 gttcgagaac ggctgggtgt cggtcggctg gaacatcgcc ggcttccacg accgcgactt
     3241 cgaggaggcc cgctacacgc gcaacggccc ctatgtgacg atgcgcttca agttcgacca
     3301 gctcacgctg cagtcgctgc tggggaaccg gtgatgcggc tgctgcgcct cctcctgttc
     3361 gtcctcgccg ccttcggctt cggctcggcg gcgcaagcgc agctgacctt ccagctgcgc
     3421 accggcgatc ccgcgaccaa catccagtcg atgccgatcg acagcaacaa ttgcgccgcc
     3481 gcgggccccc gcacgatgta tgtcggcggc gtcatcacca acacgggcct gacgacggtc
     3541 aacaacatcg tcgcgaccat gagcggagtg accggcaact tcgcgctcgg ccaggccccc
     3601 gggcagacgg tcggctcgct cggtcccgga cagagcaccg gggtctattg gcaggtcgac
     3661 tatggctgca gcgtcgccac catcacggcg acggtgagcg tcgcctcgag cggagccccg
     3721 gtttcgggaa tcgtcggcct gcgcacggtc agctcgatca gcgccaatgc cggcggcaac
     3781 gtcctctcct cgacgctcgg gccgggagcc gtggtcggcc agaccatctg gttcgacgct
     3841 tcctacgatt tcggaggcac cgacatcggc gacgagttca tcctccagcc ggccggcaac
     3901 ccgtcgttcg acgccgtctg cttccggctg gtcgggaccg agatacgcag cagcaacatc
     3961 gctccgatca ccgtcgggac gatgaaccgc atctacttcc gccagaccca gaagcagtcg
     4021 ggcaacggct atttcgccag cgtgcgctat tatttcgaat atcagtgcgc cggcgcgtcg
     4081 accacggccc ggccctatgc gaccatgacc tcgggcaacg cgctcaaata taccggcaat
     4141 tacgacggag cgagcagctt cagcatcacc ttcccgggcg cgaccaaccc gttcacgatc
     4201 agcaagaccc aggacaccgc cagcgccatc gccggcaatt cggcgacggt caaatatacg
     4261 atcacggtca gcaacccctc ggcctatgcg agccggatct cccagttcgt cgacactctg
     4321 cccgcgggcg cgaagttcct cgcgctcgac tcggcgagca gcgtcaccgc cgccaattcg
     4381 agcagcgtgc cggccgccaa cgcgaccggg accctgacct tcaccgggcg gcaggaccag
     4441 tcctacctga tcgcggcagg cgggacggtc agcctgatct acacggtcca gatgccgtcg
     4501 accgccggaa gctacaccaa cagcgccagg gccgtgttcg gcacggcgag cacctcgacg
     4561 gcgaccgcga ccttcaacgt cgtcacgccg gcgccgctca agatggagaa gtcgagccag
     4621 gcgagctccg atccggtcaa cggcaccgcc aacccgaagc tgatcccggg cgggcgcgcc
     4681 gcctatacgg tgacgatcac caaccccaat tcgttcgcga ccacggccga cagcatcgtc
     4741 atcctcgacg gcacgccggc gaacctctcg ctgttcgtca gccatgtctc cggctcgagc
     4801 ggcggcccga tccggttcca gaacggcagc accccgtccg gcctgaccta taccttcacc
     4861 agcctcgcga gcacgaccga cgacgtcgac ttctcgaacg acggcggggc gacctggacc
     4921 tatgtgccga cccccaacgc ccagggcgcg gaccccgccg tgaaccgggt ccggatccgt
     4981 ccgaaggggg cgatggcggc caattccagc ttcagcctgc tgttcggcta cgtcatcagg
     5041 tgagtggtgc ccccggccgg aatcgaaccg gcactccttg cggaactcga ttttgagtcg
     5101 agcgcgtcta ccaattccgc cacaggggca cggccgacct gctagcgcag ggcttcgggg
     5161 tcggcaaccg ccccggtcgc gcctttgccc ggacgagccc cgttcggggc ttccggccgg
     5221 caaaggaaag gatgccactt ctcgcgcatc cccagcttgc gctcgacggg cctgtccttc
     5281 gcgccgagtc gcccggttca gttgcgaggc gcctgcctcc ggtagctgag cgcctcggcg
     5341 acgtggatcc ggcgtacctc ctcgctcccg gcgaggtccg cgaccgtgcg cgcgacgcgc
     5401 atgactcggt ggaagccgcg ggcggagagg cgcatcgcct cggccgcctg ggcaagcagg
     5461 cggcggccgg gctcgtcggg agtcgccacc gcctcgagcc ggtcgccgtc cacctcggcg
     5521 ttcgttcgga tgccctcccc ttcaaagcgt cgagtctgga tcgctcgggc cgctccgacg
     5581 cggcgcgcga cctcggccga gccttccgaa ggcggcggca ggacgaggtc ggcggcggcg
     5641 acggcctgga cg
//
LOCUS       DASVQZ010000241         4554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_725013, whole genome shotgun sequence.
ACCESSION   DASVQZ010000241 DASVQZ010000000
VERSION     DASVQZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725013"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /gene="tig"
                     /locus_tag="VF547_06515"
     CDS             complement(<1..1458)
                     /gene="tig"
                     /locus_tag="VF547_06515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX8262509.1"
                     /translation="MQTVETLNQGLKRAYRITIPKADIDARVDGQLRKVAPQIRMPGF
                     RPGKVPPNLVRKMHGPAIEQEALQNAVQEGVQQLLTEQKLRPAMQPQVELEDGGPGRD
                     AVIRVELETLPEVPEPRIDDLKLERLLVEPGDAEIDAALARLAEGQKLFEPAPATQPA
                     RRGDLVVIDYEGRVDGELFEGGKGESMRVELGSGRLIPGFEDQLVGAKANEQRTVTVT
                     FPDDYPVDYLKGREAVFEVTIGEVQTARETRIDDGFAQSMGLEGIDQLKELMKGQIEQ
                     ELGGLTRTHMKRKLLDRLAETHDFEVPPSMVDAEFNQIWQQLEQEAAQEPDPAAARAE
                     MESEREEYRAIAERRVRLGLLLSEIGQRHQIQINENEMNQLIMQAAQQYRPEDRQRFV
                     EYVRNEPMAAAQLRAPLFEDKVVDFLFSKAEISERKVSRETLQAEIESTDTGHVHGPD
                     CDHDHDHAPKAKKKPAKKAKADSSASEPEAKPAKKG"
     gene            complement(1522..1606)
                     /locus_tag="VF547_06520"
     tRNA            complement(1522..1606)
                     /locus_tag="VF547_06520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Leu,seq:tag)
     gene            1731..2675
                     /locus_tag="VF547_06525"
     CDS             1731..2675
                     /locus_tag="VF547_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEX8262510.1"
                     /translation="MRYRRLGSSDLQVSEISLGSWLTFGVGVERDRAAAVVDAAFGMG
                     VNFIDTANIYGRGAAESFLGEALAGRPRDSYVLATKLYFPMTETDRGLSSAQVDKQLD
                     ASLKRLRTDFVDLYQCHRYDQETPLEETMTALSRAVRSGKVRYLGFSEWPADKIRAAI
                     EMAGVEKFVSSQPQYSLLWREPEREVIPLCAANGISQIVWSPLAQGVLTGKYRPGRAP
                     PPDSRATSAEMGGFIAWLMRDETLAAVERLEPLAAEAGLSLAQFALAWVLREPNVASA
                     IVGASRPEQLAENAAASGAEVDPALFAKAEAIVAEVGR"
     gene            complement(2680..3357)
                     /locus_tag="VF547_06530"
     CDS             complement(2680..3357)
                     /locus_tag="VF547_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262511.1"
                     /translation="MSNADGGLVLFGRKARLPVGRGRILSALVAGALLAGCAGGPGGG
                     YGLRYYRLIEPGPEEVARGSMVVTPTMRWNRAPRGPDQIFRQEDWTLNGPLLDGLSFI
                     GGLEHDKRIVQQRRKADRKVPNFRSDMSQPEIGSMIESFYRVRAGASQFNVTGLQPRQ
                     FAGSPGFQYDFEYLGGDEVWRRGRAVGAIVEGRLYLALLDAARAHYFAAALPEFERIV
                     ESARIRR"
     gene            complement(3368..>4554)
                     /locus_tag="VF547_06535"
     CDS             complement(3368..>4554)
                     /locus_tag="VF547_06535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8262512.1"
                     /translation="GGLGCAWCLGLAGRADARISPRDMTPLIQAGYKPTETDEKGLWQ
                     QYERVEEEIAGSNLLIQDKALTSYLGGLAERVGGPATKDLRVYLAHVPEFNAFMAPTG
                     FMVVFSGLLTRMRDEAQLSGVIGHEAGHFLRRHHIRGWRDLRRKSDAFSILAMGAGVA
                     GGAAGVYTGDAVQLAQIGTILSLFAYSRELEAEADAMGLKQIAAAGYDPAAMPETWQQ
                     LIEEIEASARIRKKRPRRGYSLLATHPAPAQRMADLRASAREVARPGAAERGRERYLA
                     ALGAHRKTFLDDQVKLNDPGASLYILSNLAKDGWSGLLRYYEGEVWRLRGQRGDAALA
                     GRSYAAAVAYPDAPAEAWRAHGYQLLKEGRRDEGMAALRRYLAQAPAAPDAAMVRHTL
                     GQ"
BASE COUNT          618 a         1580 c         1613 g          743 t
ORIGIN      
        1 gcccttcttc gccggcttcg cctcgggctc ggaggcggac gagtccgcct tcgccttctt
       61 cgccggcttc ttcttggcct tgggcgcgtg gtcgtggtcg tggtcgcaat cggggccgtg
      121 gacgtggccg gtatcggtcg attcgatctc ggcctgaagg gtctcgcggc tcaccttgcg
      181 ctcgctgatc tccgccttcg agaacaggaa gtcgaccacc ttgtcctcga acagcggcgc
      241 gcgcagctgg gcggccgcca tcggctcgtt gcggacatat tcgacgaacc tctgccggtc
      301 ttccgggcgg tattgctggg ccgcctgcat gatcagctgg ttcatctcgt tctcgttgat
      361 ctggatctgg tggcgctggc cgatctcgga gagaagcagg ccgagccgaa cccgacgctc
      421 ggcgatggcg cgatactcct cgcgctcgct ctccatctcg gcccgggccg cggccggatc
      481 gggctcctgg gccgcctcct gctcgagctg ctgccagatc tggttgaact cggcgtcgac
      541 catcgagggc gggacctcga aatcgtgggt ctcggcgagc cggtcgagca gcttgcgctt
      601 catgtgggtg cgggtcagcc cgcccagctc ctgctcgatc tgccccttca tcagctcctt
      661 gagctggtcg atcccctcca gccccatcga ctgggcgaag ccgtcgtcga tccgggtctc
      721 gcgcgcggtc tggacctcgc cgatcgtgac ctcgaacacc gcctcgcggc ccttcagata
      781 gtcgaccgga taatcgtcgg ggaaggtgac ggtgaccgtc cgctgctcgt tcgccttggc
      841 cccgaccagc tggtcctcga agccggggat caggcggccc gagccgagct cgacgcgcat
      901 cgattcgccc ttgccgcctt cgaacagctc gccgtcgacc cggccctcat aatcgatgac
      961 gacgagatcg ccgcgccgcg ccggctgcgt cgccggcgcc ggctcgaaca gcttctggcc
     1021 ctcggcgagc cgggccagcg ccgcgtcgat ctcggcgtcg ccgggctcga ccagaagccg
     1081 ctcgagcttg aggtcgtcga tcctcggctc gggcacttcg ggaagggtct cgagctcgac
     1141 ccggatcacc gcgtcgcggc ccgggccgcc gtcctcgagc tcgacctggg gctgcatcgc
     1201 gggccgcaac ttctgctcgg tgagcagctg ctggaccccc tcctggaccg cgttctggag
     1261 ggcttcctgc tcgatcgccg ggccgtgcat cttcctgacc aggttgggcg gcaccttgcc
     1321 ggggcggaag ccgggcatgc ggatctgcgg cgcgaccttc ctgagctggc cgtcgactcg
     1381 ggcgtcgatg tcggcctttg ggatggtgat gcggtaggcg cgcttgaggc cctggttcaa
     1441 cgtctcgacg gtctgcatga tctgaagctc tttgccttct cgcgggagtg cggccttcgt
     1501 cgggatcggg cggagcggga ctggtgcggg cgaagggact cgaaccccca catctttcga
     1561 tactggaacc taaatccagc gcgtctacca gttccgccac gcccgccggg ccgccggtcg
     1621 gcggcggata tagcaggcgt ggcgcggggg gcaagggacg gcgtcggcga gcggccctgg
     1681 aacgattgcc gggctcgagc ctttagcggg agcgaggagg cgggcggggc atgcgctacc
     1741 ggcggctcgg atcgagcgat cttcaggttt cggaaatctc gctggggtcg tggctgacct
     1801 tcggcgtggg cgtcgagcgc gaccgggccg cggcggtggt ggatgcggct ttcgggatgg
     1861 gcgtcaactt catcgacacc gcgaacatct acgggcgggg agcggcggaa agcttcctcg
     1921 gcgaggcgct cgccgggcgg ccgcgcgata gctacgtgct cgcgaccaaa ttgtacttcc
     1981 cgatgacgga gaccgaccgc ggcctctcga gcgcccaggt cgacaagcag ctcgacgcct
     2041 cgctcaagcg gctgaggacc gacttcgtcg acctctacca gtgccaccgc tacgaccagg
     2101 agaccccgct cgaggagacg atgacggcgc tcagccgcgc ggttcgctcg ggcaaggtgc
     2161 gctatctcgg cttctccgaa tggcccgccg acaagatccg ggcggcgatc gagatggcgg
     2221 gggtcgagaa gttcgtctcc agccagcccc aatattcgct gctgtggcgc gagccggagc
     2281 gggaggtgat cccgctctgc gccgccaacg gcatctcgca gatcgtctgg tcgccgctcg
     2341 cccagggggt cctgaccggc aagtaccggc ccggccgggc gccgccgccg gacagccggg
     2401 cgaccagcgc cgagatgggc ggcttcatcg cctggctgat gcgggacgag accctggcgg
     2461 cggtggagcg gctggagccg ctcgcggcgg aagccgggct cagcctcgcc cagttcgccc
     2521 tcgcctgggt gctgagggag cccaacgtcg cctcggcgat cgtcggcgcc tcgcgacccg
     2581 agcagctggc cgagaatgcg gccgcctcgg gcgccgaggt cgacccggcg ctgttcgcca
     2641 aggccgaggc gatcgtcgcg gaggtcgggc gctagcgccc tagcgccgga tccgggcgct
     2701 ctccacgatc cgctcgaact ccggcagcgc ggcggcgaaa taatgggcgc gggcggcgtc
     2761 gagcagggcg aggtagagcc ggccctcgac gatcgcgccc accgcccgcc cgcgccgcca
     2821 gacctcgtcg ccgccgagat attcgaaatc gtactggaag ccggggctgc cggcgaactg
     2881 gcgcggctgc aggccggtga cgttgaactg gctcgccccg gcccgcaccc ggtagaagct
     2941 ctcgatcatc gagccgatct cgggctggct catgtcggag cggaaattgg gcaccttgcg
     3001 gtcggccttg cggcgctgct ggacgatgcg cttgtcgtgc tcgaggccgc cgatgaagct
     3061 gaggccgtcg agcagaggcc cgttcagggt ccagtcctcc tgccggaaga tctggtccgg
     3121 cccgcgcggc gcccggttcc agcgcatcgt cggggtcacc accatcgagc cgcgcgcgac
     3181 ctcctccggc ccgggctcga tcagccggta gtagcgcagg ccgtagccgc cgcccgggcc
     3241 gccggcgcag cccgcgagca gcgcgccggc gaccagcgcc gaaaggatcc tgccccgccc
     3301 gaccgggaga cgcgccttcc ggccgaaaag gacgagtccc ccgtctgcgt tcgacatcct
     3361 tcgcctccta ttggccgagc gtgtggcgga ccatcgccgc gtcgggcgcc gccggcgcct
     3421 gcgcgagata gcggcgcagg gccgccatcc cctcgtcgcg gcggccctcc ttgagcagct
     3481 ggtagccgtg ggcgcgccag gcctcggccg gcgcgtccgg ataggcgacg gcggcggcgt
     3541 agctccggcc ggccagagcg gcgtcgccgc gctgcccgcg cagccgccac acctcccctt
     3601 cgtaatagcg cagcaggccg ctccagccgt ccttggcgag attgctcaat atgtagaggc
     3661 tggcgccggg atcgttgagc ttgacctggt cgtcgaggaa cgtcttgcgg tgcgccccga
     3721 gcgcggcgag gtagcgctcg cggccgcgct cggcggcgcc cggcctcgcc acctcgcggg
     3781 cggaggccct gagatcggcc atgcgctggg ccggggcggg atgggtcgcg agcagggaat
     3841 agccccgcct cggccgcttc ttccttatcc gggcgctggc ctcgatctcc tcgatcagct
     3901 gctgccaggt ctcgggcatg gcggcgggat cgtagccggc ggcggcgatc tgcttgaggc
     3961 ccatggcgtc ggcttccgcc tcaagctcgc ggctataggc gaagagggag aggatcgtgc
     4021 cgatctgcgc cagttggaca gcatcgcctg tatagacacc ggccgccccg ccggcgacgc
     4081 cggcacccat tgcgaggatg ctgaaggcgt cggactttcg gcgaaggtcg cgccagccgc
     4141 ggatgtgatg ccggcgaagg aagtgaccgg cctcatgccc gatcacgccc gaaagctggg
     4201 cctcatcgcg catccgggtg aggagccctg agaagaccac catgaagccg gtcggcgcca
     4261 tgaaggcatt gaactccggc acgtgcgcca gatagacgcg cagatccttc gtcgcgggcc
     4321 ctccgacccg ctcggcaagc ccgccgagat aggaggtcag cgccttgtcc tggatgagga
     4381 ggttggagcc ggctatctcc tcctccaccc tctcatattg ctgccagagg cccttctcgt
     4441 cggtctcggt gggcttgtag ccggcctgga tcagcggggt catgtcgcgc ggggagattc
     4501 gcgcgtcggc tcttcctgcg aggccgagac accaggcgca gccgaggccg ccga
//
LOCUS       DASVQZ010000242         5121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_725140, whole genome shotgun sequence.
ACCESSION   DASVQZ010000242 DASVQZ010000000
VERSION     DASVQZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725140"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="rsmH"
                     /locus_tag="VF547_06540"
     CDS             <1..925
                     /gene="rsmH"
                     /locus_tag="VF547_06540"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX8262513.1"
                     /translation="EVIAALDVAAGETHVDGTFGAGGYTKAILEKGAAKVYAFDRDPD
                     AIEGGEALAASSGGRLTLVPERFSRMRQALADRDVDQVDGVTLDIGVSSMQLDQAERG
                     FSFQSDGPLDMRMGREGESAADFLNGAPEEVIADVIYRYGEEPKSRRIARAIVAARPV
                     ERTAQLAAIVRKALGWHSGMKKDPATRTFQAIRIHLNEELQELEGGLAAVEQVLKPGG
                     RLAVVTFHSLEDRMVKRFLKERSGDVPSGSRHLPDRAASRPASTFDSVAKPVRAGEAE
                     VKANPRARSATLRVARRTDANAWTSREGMPS"
     gene            922..1734
                     /locus_tag="VF547_06545"
     CDS             922..1734
                     /locus_tag="VF547_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262514.1"
                     /translation="MIARGFKSVIWVATVGSAALGCYMVSLRVATERNELAKVERQII
                     AAKRDIRSLQTELGTRGRMTQLEQWNAEVLALSAPSSAQFLKDEFTLARLYRNDPTVE
                     ERAAEVRLASADTGAAKAGGTPKAATPPVVQAVAERPAAQAPMIHRASFTTGEAPAKA
                     KPAPAQPERVRTAAATAGTDKPVKPAAAKTASVAQPKPDRSAAKPSDNAPKPAAQRTA
                     SASAKPESGNSRPAAKPSAKPAAKGETLRTAAAKPAREAAPRSAKVKGGGGN"
     gene            1734..3428
                     /locus_tag="VF547_06550"
     CDS             1734..3428
                     /locus_tag="VF547_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8262515.1"
                     /translation="MASAAILTDRYGGAGHRPQGLSLTYQRLMLMMLVFAGVTMLIAG
                     RLLFLQLFTDRGGAGAIGNPLIPPRADIVDRNGTPLARTIDAWSIAIHPDRLLGNPAE
                     LAVKLAQLMPERSAADYKAMLTSGKTFLYLSRRAVPELVAAVNALGEPAIEFSREPQR
                     MYPQTAMAGHILGWTDFDGNGVAGMERVLQDRLSDPAQRGTPVALSIDARVQGVMEAE
                     LSAAMTKFSAIGAAGIVLDIASGEVIAMTSLPQLNPNKPGRNAEQAGFNRATLGVYEL
                     GSTFKPFTVAMAMDTGHVKSFGQMYNCPSGLRAGGFTITDTHPFGRACSVAEIMKESS
                     NIGTAQIAAQVGSAKQQEFLRKMGFLEPISIELNERGRTLTPGANWGDVATMTVGYGH
                     GIAVTPLHLANGYATLFNGGIWRPTTLLKHDRNHPVPAGRRVFSEETSYRMRSLLRLV
                     VTDGTGGKADAKGYRVGGKTGTAEKIVGGRYTGAAVVTTFAGVFPMDNPRYVVVAMLD
                     DPKATAETYGFHTAGWNVAPVVSKVVSRIGALLGVQPDERRDVNMSEVLQYVKKEG"
     gene            3432..4847
                     /locus_tag="VF547_06555"
     CDS             3432..4847
                     /locus_tag="VF547_06555"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX8262516.1"
                     /translation="MRLADLAGIGGDSRVTGFAIDHRKVAPGTIFGAFRGSVFNGEDF
                     IPQAISAGAVAVVARPDVSVAGAAHVAAEEPRREFARLAAKFFEPFPETVVAVTGTNG
                     KTSSVELTRQLWRMAGHHAASIGTLGVTTADDRTTTGLTTPDIVTFLSNMAGLAREGV
                     THAAFEASSHGLSQYRTEGLPVRAAAFTNLSRDHLDYHETMEAYFEAKMRLFAEVVEP
                     DGAAVIWTDDPRSDEVARRAGARGLRLLTVGRRGETLKLAGREPTQLGQRLTIEADGK
                     AHKVELPLIGAYQAANALTAAGLVLATGGELGQVLASLARVQGVRGRLERAVITRAGA
                     PVYVDYAHTPDAIEAAIEALRPHSKGRLIIVFGAGGDRDVGKRAEMGAVASRLADLAI
                     VTDDNPRSEDPAAIRGAVLRGAAGAREVPGRREAIAAAVAEAGPDDIILLAGKGHEQG
                     QIVGDKVLPFDDVTVARECAA"
     gene            4844..>5121
                     /locus_tag="VF547_06560"
     CDS             4844..>5121
                     /locus_tag="VF547_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEX8262517.1"
                     /translation="MSPLWTSSEIAAATGGQVRGTFEVTGVSFDSREVEPGHLFVAMK
                     GEAMDGHRFVQGAFEAGAAGAMVSEEIAQPHVLVADTMEALNALGRASR"
BASE COUNT          840 a         1819 c         1753 g          709 t
ORIGIN      
        1 tgaggtcatc gccgcgctcg acgtcgccgc cggcgaaacc catgtcgacg gcacgttcgg
       61 cgccggcggc tacaccaagg ccattctcga aaaaggggcc gccaaagtct acgccttcga
      121 tcgcgatccc gatgcgatcg agggcggcga ggctctggcg gcttcgagcg gggggcgcct
      181 gacgcttgtt ccggagcgtt tttcccggat gcgtcaggcg cttgccgacc gcgacgtcga
      241 ccaggtggac ggggtgaccc tcgacatcgg tgtttcttcg atgcagctcg accaagccga
      301 gcgcggcttc tccttccagt ccgacgggcc gctcgacatg cgcatgggcc gggaggggga
      361 gagcgccgcc gatttcctca acggcgcccc cgaagaggtg attgcggacg tcatctaccg
      421 ctacggcgag gagcccaagt cgcgccggat cgcccgggcc atcgtcgccg ctcggccggt
      481 ggagcgcacg gcgcagctcg ccgcgatcgt ccgaaaggca ctgggctggc attcggggat
      541 gaagaaggat ccggcgaccc gcaccttcca ggcgatccgg atccacctca acgaagagct
      601 tcaggagctg gaaggcggcc tcgccgcggt cgagcaggtg ctcaagccgg gcggaaggct
      661 cgcggtcgtc accttccaca gcctcgagga ccggatggtg aagcgattcc tcaaggagcg
      721 gagcggagac gttccctcgg gatcgcgcca cctgcccgac cgggcggcaa gccgcccggc
      781 ctccaccttc gactccgtcg ccaagccggt ccgtgccggc gaggccgagg tcaaagccaa
      841 tccgcgggcc cgctcggcga cgttgcgagt ggcccgccga accgacgcaa acgcttggac
      901 gagcagggaa gggatgccct catgatcgca aggggcttca agtccgttat ctgggtcgca
      961 accgtcggca gcgccgctct gggctgctac atggtgtcgc tccgggtcgc gaccgaacgc
     1021 aacgagctcg ccaaggtcga acggcagatc atcgccgcca agcgcgacat acgctcgctc
     1081 cagaccgagc ttggaacccg cggccggatg acccagctcg agcagtggaa cgccgaagtc
     1141 ctcgccttgt cggcgccgag ctcggcccag ttcctcaagg acgagttcac cctcgcccgg
     1201 ctctaccgca acgacccgac ggtcgaggag cgtgcggccg aggtccgcct cgcctccgcc
     1261 gataccggtg ccgccaaggc cggagggacc ccgaaggcgg cgactcctcc ggtcgtgcag
     1321 gcggtcgccg agcgccccgc ggctcaagcg ccgatgatcc accgggcgag cttcacgacg
     1381 ggcgaggctc cggccaaggc caagccggct ccggcccagc cggagcgcgt tcggaccgcc
     1441 gcggcgacgg ccgggacgga caagccggtc aagcccgccg cagccaagac ggcttccgtt
     1501 gcacagccca agcccgatcg atccgctgcc aagccgtccg acaatgcgcc caagccggcc
     1561 gctcagcgaa cggcctcggc gtcggcgaag cccgagagcg gaaactcccg cccggccgcg
     1621 aagccctcgg ccaagccggc cgccaagggc gagactctgc ggaccgcggc cgccaagccg
     1681 gcgcgcgaag cggctccccg ctccgccaag gtcaagggag gcggcggcaa ctgatggcca
     1741 gcgcggcgat cctcaccgac cgctacggcg gcgcaggcca ccgcccgcag gggctgagcc
     1801 tcacctatca gcgcctgatg ctgatgatgc tggtcttcgc cggagtgacc atgctcatcg
     1861 ccgggcggct cctgttcctc cagctgttca ccgaccgcgg cggcgccgga gcgatcggca
     1921 atccgctcat cccgccgcgt gcggacatcg tcgaccgcaa cgggactccg ctggcccgga
     1981 cgatcgacgc ctggtccatc gcaatccatc ccgaccggct gctcggcaat cccgccgagc
     2041 tcgcggtcaa gctcgcccag ctgatgccgg aacggagcgc ggccgactat aaggcgatgc
     2101 tgacctccgg caaaaccttc ctctacctca gccgccgtgc cgttcccgag ctggtcgcgg
     2161 cggtgaacgc gctgggcgag ccggcgatcg agttcagccg cgagccgcag cgcatgtacc
     2221 cgcagaccgc gatggccggt catatcctgg gatggacgga tttcgacggc aacggagtcg
     2281 ccgggatgga gcgcgtgctc caggaccggc tcagcgaccc ggcccagcgc ggaaccccgg
     2341 tcgctctgtc gatcgacgcc cgggtccagg gcgtgatgga ggcggagctc agcgccgcga
     2401 tgaccaagtt cagcgcgatc ggcgccgccg ggatcgtgct cgacatcgcg agcggcgagg
     2461 tcatcgcgat gacgtcgctt ccgcagctca acccgaacaa gccgggacgc aatgcggagc
     2521 aggccggctt caatcgcgcc accctcggag tctacgagct cggctcgacc ttcaagccgt
     2581 tcaccgtcgc gatggcgatg gacaccggcc atgtgaagag cttcggccag atgtacaatt
     2641 gcccgtcggg ccttcgcgcc ggcggcttca cgatcaccga cacccatccg ttcggccggg
     2701 cctgcagcgt cgccgagatc atgaaggaaa gctcgaacat cgggaccgcc cagatcgccg
     2761 cccaggtcgg atcggcgaag cagcaggaat tccttcgcaa gatgggcttc ctcgagccga
     2821 tttcgatcga gctgaacgag cgcggccgga ccctgacccc gggcgccaac tggggcgacg
     2881 ttgcgacgat gacggtcggc tacggccatg ggatcgcggt cactccgctc catctcgcca
     2941 acggctacgc caccttgttc aacggcggga tctggcggcc gaccaccctc ctcaagcacg
     3001 accggaacca ccccgtcccg gcgggccggc gggtgttcag cgaggagacg tcctaccgga
     3061 tgcgctcgct gctccggctg gtcgtcaccg acggcaccgg cggcaaggcc gacgccaagg
     3121 gctatagggt gggcggcaag acgggaacgg ccgagaagat cgtcggcggc cgctataccg
     3181 gagccgcggt cgtcaccacc ttcgcgggcg ttttcccgat ggacaacccc cgctatgtgg
     3241 tcgtcgcgat gctcgacgat cccaaggcga ccgccgaaac ctacggcttc cacaccgcgg
     3301 gctggaacgt cgctccggtc gtgtccaagg tcgtgagccg gatcggcgcc ctgctcggag
     3361 tccagccgga cgagcgccgc gacgtcaaca tgtccgaggt tctccaatac gtcaaaaagg
     3421 aaggctgatc gatgcgcctc gccgacctcg cgggaatcgg aggcgattcc cgggtgacgg
     3481 gcttcgcgat cgaccaccgc aaggtcgctc cgggaacgat cttcggcgcc tttcgcggga
     3541 gcgtcttcaa cggcgaggat ttcatccccc aggcgatcag cgccggagcg gtcgcggtgg
     3601 tcgcccggcc ggacgtgtcc gtggccggtg cggcgcatgt cgccgccgag gagccgaggc
     3661 gggagttcgc ccggctcgcc gctaaattct tcgagccctt ccccgagacg gtcgtcgccg
     3721 tcaccggaac caacggaaag acgtcgagcg tcgagctgac ccgccagctg tggcggatgg
     3781 ccgggcatca tgccgcatcc atcggcaccc tcggagtgac gacggccgac gaccggacca
     3841 cgaccggcct gacgacgccc gacatcgtca ccttcctgtc gaacatggcc gggctcgcgc
     3901 gcgagggggt cacccacgcc gccttcgagg cttcgagcca cggcctttcg caataccgga
     3961 cggaggggct ccccgtccga gcggccgcct tcaccaacct cagccgcgac catctcgatt
     4021 atcacgagac gatggaggcc tatttcgagg ccaagatgcg cctgttcgcc gaagtggtcg
     4081 aaccggacgg agcggcggtg atctggaccg acgacccgag gtcggacgag gtcgcccggc
     4141 gggccggagc gcgcgggctc aggctgctga ccgtcggccg aaggggcgag accctgaagc
     4201 tcgccggccg ggagccgacc cagctcggcc agcgcctgac gatcgaggcc gacggcaagg
     4261 cccacaaggt cgagcttccg ctgatcggcg cctatcaggc ggccaatgcc ctcaccgccg
     4321 ccgggctggt ccttgccacc gggggtgagc ttggccaggt cctcgccagc ctggcccggg
     4381 tgcagggcgt gcgcggccgg ctcgagcggg cggtgatcac ccgcgccggg gcgccggtct
     4441 atgtcgatta tgcccatacg cccgacgcga tcgaggcggc gatcgaggcg ttgaggccgc
     4501 attccaaggg ccggctgatc atcgtgttcg gagccggcgg cgaccgcgac gtcggcaagc
     4561 gggcggagat gggcgcggtc gcttcccgac tggccgacct cgccatcgtc accgacgaca
     4621 atcccaggag cgaggatccg gccgcgatcc gtggcgccgt cctgcggggc gctgccggtg
     4681 cccgcgaagt gccgggccgc cgcgaagcca tcgccgccgc cgtcgccgag gccgggccgg
     4741 acgacatcat cctcctcgcc ggcaaggggc acgagcaggg ccagatcgtc ggcgacaagg
     4801 tgcttccctt cgacgacgtg accgtcgctc gcgagtgcgc ggcatgagcc ccctctggac
     4861 ctcgtccgaa attgccgccg cgacgggcgg acaggttcgg ggtaccttcg aggtcaccgg
     4921 agtgagcttc gactcccgcg aggtggagcc gggccatttg ttcgtcgcga tgaagggcga
     4981 ggccatggac ggccatcggt tcgtgcaggg cgctttcgag gccggggccg cgggcgccat
     5041 ggtctccgag gaaatcgcgc agccccacgt cctggtcgcc gacaccatgg aggcgctgaa
     5101 cgctctcggc cgcgcatcgc g
//
LOCUS       DASVQZ010000243        12009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_725168, whole genome shotgun sequence.
ACCESSION   DASVQZ010000243 DASVQZ010000000
VERSION     DASVQZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725168"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VF547_06565"
     CDS             <1..236
                     /locus_tag="VF547_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262518.1"
                     /translation="LLRPGLATGGTTFTGTAGQGRLFLGLLALVGLFGALSAVNGAYM
                     IATGRRNAVRVRVVLALVLLLFAIGWAVRRGLV"
     gene            290..727
                     /locus_tag="VF547_06570"
     CDS             290..727
                     /locus_tag="VF547_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262519.1"
                     /translation="MRHPLLALLLAAAAETAALACSCRQPPDTEAGRQRLAREIAQGA
                     SALVEVELVRPYDDRTGRGERLRVRRTLAGRAPPLVDLERHGRPESAACQQEFLPGRR
                     RIVMLYPPQPRSALHRVGSSCTTFLLSDPPFLRAVTEAMRRRR"
     gene            complement(699..1853)
                     /locus_tag="VF547_06575"
     CDS             complement(699..1853)
                     /locus_tag="VF547_06575"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8262520.1"
                     /translation="MRSLFPLLLLAACSASNGSDAAAQPASSPTRPFTVRSITTFDEP
                     WAMAFLPGPGTRALITEKKGRLKLLWNEIGRVGDVAGAPTVDYGGQGGFGDVVLHPGF
                     ARNRLVYLSWVEAGEGRNRGAVVGRARLVEGDGKGVRLDGLQVIWRQTPKMSGRGHYG
                     HRIAFGPDGKLYISNGDRQEFTPAQDMGGTLGKIVRLNDDGTVPRDNPFASRGGAAAQ
                     IWTLGHRNPLGLAFDGRGRLWDVEMGPKGGDELNLVLRGKNYGYPIVSNGDHYDGRDI
                     PDHPTRPEFEAPKVTWTPVISPGDMIFYSGNAFPQWKGDALIAGLSSKALIRVDIDGD
                     KAREAERWDMGQRIREVEQGPDGSVWLLEDGPGGRLLKLSPASGGGASPR"
     gene            complement(2259..2489)
                     /locus_tag="VF547_06580"
     CDS             complement(2259..2489)
                     /locus_tag="VF547_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262521.1"
                     /translation="MPRYFFNVRGSGFEETDLVGRTCADDVAALAEALSEAGRVLRDL
                     LREDALSDKGEIEIEDERHRTILTLPLRAAAY"
     gene            2813..4570
                     /locus_tag="VF547_06585"
     CDS             2813..4570
                     /locus_tag="VF547_06585"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8262522.1"
                     /translation="MAIINGTHKADTIIGTAQDDSIFGDNGNDTINGGAGNDTIDGGN
                     GSDLISGGAGNDVIDGGNGQDTIVYAGNRADYRVMQGLNGAVIVRDLRSGRPDGEDRL
                     FSAERIQFADGTFKLVDLIEANAAPVARNDALTLSETAGKTEVTPTLLANDSDPDGDS
                     LVVSSVQAVSAQGAIVTLGPDGKVSYDAGSIFADLDNGQTATDSFTYTITDGNGHVST
                     ATATVTITGVTQNEAPVAGNDQLTLAEDAGATDVTAQLLANDSDPDGDAFKVTEVQAV
                     SAKGAAVTVDKDGVVNYDAGKIFVYLEEGQTATDTFTYTITDSAGLTSTATATVTITG
                     VTPEVEMPEWDTWFYVSEDGTNDEMWGWIVESFGFDIAGVDASHALGTVEFNYEQGLL
                     SFTADHPSSDVLNYDQHQDTWFTVIGDNGKKITVGMVIEGVNDAIVAVDDQVAVGEGQ
                     TTGNLYSTLVSNEIDPDGGANSNRILSVDTTGTQGSVSFDASGRTVIYSAAGLDLAPG
                     ETLVDSFTYLVEDGFHSTDTATVFVTVTGAADGSVSASMSDSLMAAFLPAGGSGGHDL
                     PTFDMAQAQMILGADAYIA"
     gene            4850..5359
                     /locus_tag="VF547_06590"
     CDS             4850..5359
                     /locus_tag="VF547_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX8262523.1"
                     /translation="MTDIFGSNPFDIVPHLAALAVAYLLAFPLGWNREREERSAGLRT
                     FPLVAVAACGFVQATETLTVGEPNAMARILEGIIAGMGFIGGGAILRQKDSVKGTATA
                     ASLWATGAIGTAVGLGSYDVAIVLSLITLLTLWVLSPLKPDLVQEPAGGAAAGPSARE
                     EEERAESQS"
     gene            complement(5443..6468)
                     /locus_tag="VF547_06595"
     CDS             complement(5443..6468)
                     /locus_tag="VF547_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8262524.1"
                     /translation="MHAPLSPAPILQLGMGFFASKVLLSAVELGLFTELAGESRTGAE
                     IEQALGLHPRATYDFLDTLVAVGMLERDGDGAGARYANTPATALYLDRCSPRYVGGIL
                     EMANARLYRFWADLTPALKTGQPQNEVRHSQRPMFEELYADPARLEQFMDAMRGVSMA
                     NFEAFADKFDFAPYLTMADIGGATGQLSATVAARHPHLACASYDLPVVRPIAERRLRE
                     AGLADRVAAREIDFLKEEFPSADLITMGMILHDWNLEHKKMLIAKAYRALPEGGALVA
                     IENIIDDERRQNAFGLLMSLNMLIEFGDAFDFTGADFTEWCLEAGFRRCEILPLAGPA
                     SAAIAYK"
     gene            6816..7343
                     /gene="rimP"
                     /locus_tag="VF547_06600"
     CDS             6816..7343
                     /gene="rimP"
                     /locus_tag="VF547_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation protein RimP"
                     /protein_id="HEX8262525.1"
                     /translation="MADIAEVTRLIEPAAEAEGLALVRVKMIGGTSDPTLQVMAERPD
                     TRQLTLEDCARLSRRLSELLDEADPIEHAYRLEVSSPGIDRPLTRLKDFDDWKGHEAR
                     IVLAEKLDGRKVFAGELCGIEGETVLVKVPVQGPVGLPFAAIHSAKLVMTDKLIAATA
                     PLSAEGADTIKVEED"
     gene            7346..8965
                     /gene="nusA"
                     /locus_tag="VF547_06605"
     CDS             7346..8965
                     /gene="nusA"
                     /locus_tag="VF547_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX8262526.1"
                     /translation="MATTAPGAPGAVSANKAELLAIADAVAREKLIDKAIVIEAMEDA
                     IQRAARARYGAENDIRAKLDPVSGDLRLWRVVEVCEQVDDYFKQVSLEDAQKLQPGAA
                     LGDFIVDPLPPIEFGRIAAQAAKQVIFQKVRDAERERQYEEFKERAGEIITGVVKRVE
                     FGHVVVDLGRAEGVIRRDQQIPREVVRVGDRVRSLILSVRRENRGPQIFLSRAHPDFM
                     KKLFAQEVPEIYDGIIEIKAAARDPGSRAKIGVISYDSSIDPVGACVGMKGSRVQAVV
                     QELQGEKIDIIPWSEDLATFVVNALQPATVSRVVIDEEESRIEVVVPDDQLSLAIGRR
                     GQNVRLASQLTGSAIDIMTEADASEKRQREFVERSELFQKELDVDETLAQLLVAEGFG
                     ALEEVAYVELDELASIEGFDDELATELQSRAAEALERREEASREERRALGVSDELAEM
                     PYLTEQMLVTLGKAGIRTLDDLADLATDELVQKKRPEQRRQRESNRPEDKGGILAEYG
                     LSDEQGNEIIMAARAHWFEDEGEDAVAETSQ"
     gene            9110..9784
                     /locus_tag="VF547_06610"
     CDS             9110..9784
                     /locus_tag="VF547_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HEX8262527.1"
                     /translation="MSASHTPERKCILSGEHGSPDSLIRLALSPDGEVLPDVRAKAPG
                     RGAWIGVDRPALEAARAKGKLRGALARAFKGAPLSIPDDLGERIESGLRQAALDRLGL
                     EARAGTLVTGTDRIVDAARKGEVHLLLHAADAGEDGRRKLDQAWRVGGQEGQGLVIPA
                     QRSILSLALGRQNVVHIALKDRAAAARVRHALDRWCAFIGTEAGLDAATARTPDASAV
                     DVNEGL"
     gene            9784..>12009
                     /gene="infB"
                     /locus_tag="VF547_06615"
     CDS             9784..>12009
                     /gene="infB"
                     /locus_tag="VF547_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX8262528.1"
                     /translation="MSETTDKPKLGARAPLGLKRTVETGKVKQSFSHGRSNTVVVEVK
                     KRRILGPAAAPEAKVEEAPPPAAAAARAPQPAQPAPQPAAPQRPVNDAATRRELQEKL
                     LREAEEARLNALEEARRREDKQAQQATEDEKRRAEENRRAEEERIEQARLAAEEEARR
                     ATEEPARPAEAAPAPAAEDEDEDRRPAHARPKHHAPAAKRPEPARPARGRDDRRHAGK
                     LTVTRALSDEDDSRARSLAALRRAREKEKRAHQQAGPSAKQVRDVVVPDAITVQELAN
                     RMAERAADLVKALFKMGMVVTVNQTIDQDTAELLVTEFGHNIKRVSEADVDIQADSDT
                     DAPETLQPRPPVVTIMGHVDHGKTSLLDAIRGANVVSGEAGGITQHIGAYQVALPDKS
                     KITFLDTPGHEAFTEMRARGANVTDIVVLVVAADDGLKPQTIEAINHTKAAGVPMIVA
                     INKIDKPGANAQKVREELLQHEIIVEDMGGEVQDVEVSALKKTNLDGLLEAIALQAEL
                     LELKANPDRSAEGAVVEAKLDKGRGPLATVLIQRGTLKVGDVFVVGPVPGKVRAMIDD
                     KGKQVKEAGPSVPVEVLGLSGVPSAGDTLTVVENEARAREVAAYRQSVLDRKRTTSAP
                     VSVEHMFASKASQAKEFPLVVKADVQGSAEAIVNAVNRISTEDIKARILNSGVGAITE
                     SDVILAAASGAPIIGFNVRPNAKAREAAERNKVEFRYYDVIYHLTDWVREAMGGQLGP
                     EI"
BASE COUNT         1986 a         4159 c         4215 g         1649 t
ORIGIN      
        1 ccctgcttcg gccgggcctt gcgaccggcg gcacgacctt caccggcacc gccggccagg
       61 ggcgcctctt cctcggcctg ctcgcgctgg tcggcctgtt cggggcgctc agcgcggtca
      121 acggcgccta catgatcgcc acgggccggc gaaacgcggt gcgcgtgcgc gtcgtcctcg
      181 cgctggtcct gctcctgttc gcgatcggct gggcggtcag gcgcggcctg gtgtagcgcc
      241 gcttcatcga cccgggcgca tctgctaggg cagcgtcgga ggcgtatcga tgcgacaccc
      301 gctccttgcc ctgctcctcg ccgccgcggc cgagacggcg gctttggcct gctcgtgccg
      361 gcagccgcct gacaccgagg ccggccgcca gcggctcgcg cgcgagatcg cgcagggcgc
      421 ctcggcgctg gtcgaggtcg agctcgtccg gccctatgac gaccgcaccg ggcgcggcga
      481 gcgtctgcgg gtccgacgga ccctcgccgg ccgggccccg cccctcgtcg acctcgagcg
      541 ccacggccgt cccgagtcgg ccgcctgcca gcaggaattc ctccccgggc ggagacgcat
      601 cgtcatgctc tacccgccgc agccgcgcag cgccctgcac cgggtcggca gcagctgcac
      661 caccttcctc ctgtccgacc cacccttcct cagggcggtc accgaggcga tgcgccgccg
      721 ccgctagccg ggctgagctt gagcagccgt ccgccgggcc cgtcctcgag cagccagacc
      781 gagccgtccg gcccctgctc gacctcgcgg atgcgctggc ccatgtccca gcgctccgcc
      841 tcgcgcgcct tgtcgccgtc gatgtcgacc cggatcagcg ccttgctcga caggccggcg
      901 atcagggcgt cgcccttcca ttgcgggaag gcgttacccg aatagaagat catgtcgccc
      961 ggcgagatga ccggcgtcca ggtcaccttg ggcgcctcga actcgggacg ggtcgggtgg
     1021 tcgggaatgt cgcggccgtc gtaatgatcg ccattggaga cgatcggata gccgtaattc
     1081 ttcccgcgca ggacgaggtt cagctcgtcg ccgcccttcg gacccatctc gacgtcccac
     1141 agccggccgc ggccgtcgaa ggcgaggccg agcgggttgc ggtggcccag ggtccagatc
     1201 tgggccgccg ctccgccgcg gctcgcgaac ggattgtcgc gcgggacggt gccgtcgtcg
     1261 ttgaggcgga cgatcttgcc gagcgtcccg cccatgtcct gggcgggggt gaattcctgg
     1321 cggtcgccgt tcgagatgta gagcttgccg tccggcccga aggcgatccg gtggccgtaa
     1381 tggccgcggc ccgacatctt cggggtctgg cgccagatca cctggagccc gtcgagccgg
     1441 acgcccttgc cgtccccttc gaccagccgc gcccggccga ccaccgcgcc ccggttcctg
     1501 ccctcgcccg cctcgaccca gctcagatag acgagccggt tgcgggcgaa gcccggatgg
     1561 aggacgacgt cgccgaagcc gccctggccg ccataatcga ccgtcggcgc tccggcgacg
     1621 tcgccgaccc ggccgatctc gttccagagc aatttcagcc ggcccttctt ctcggtgatc
     1681 agggcccgag tgccgggccc gggcaggaag gccatcgccc agggctcgtc gaacgtcgtg
     1741 atcgaccgga ccgtgaacgg ccgggtcgga gacgaggccg gctgggcggc ggcgtcgctg
     1801 ccgttcgagg cgctgcaggc ggcgaggagg agaagcggga agaggctgcg catcaagggc
     1861 tcctgggcgg ggaaggtcgg gaaagtccgg cttccataag cgataaagac cttccgatga
     1921 acctttcgcg ggaacggagc gcaaaagaca caaaagaccc gcgaatcggg ggcgagaatc
     1981 ctttgtcgtc agccgaggtg tcggctttgg gatcgccccg cgccgcaccg tccagacagg
     2041 cgttcgtcat tcgaccccga cggaatcgcc cgtttggaac atagcgcgaa caacctggaa
     2101 aggcaatccg ctcaaccggt cgatgagctg ttccccaggc gaaagccggg gtccggcggc
     2161 cacggccgga gaggctggat cccggcttcg ccgggaagcg gctgtgagcg gctgtaggcg
     2221 aaccgcctcg ggcgccaact gggcgaacgc gtccggtgct agtaagccgc ggcgcgcagg
     2281 ggcaaggtga ggatggtgcg gtggcgctcg tcctcgatct cgatctcacc cttgtcggag
     2341 agggcgtcct cgcggagaag gtcgcgaagg actcggcccg cctcgctcag cgcctcggcc
     2401 aaggcggcga cgtcgtcggc gcaggtgcgg ccgaccaggt ccgtctcctc gaagccgctg
     2461 ccgcggacgt tgaagaaata gcgtggcatg ggcgtcggat gcagaacatc gcgcgcgggg
     2521 agcgggttcc gcggagcggg ccggtccgtc gcttcccgaa acaattgaga cacttctctt
     2581 ccgtccgcga catcggccgg ccaagcgcgg cggcgaaacg gctgctcgct gttcggccgc
     2641 acccgcggac gattcagggg agtaagcgta agatgtcctg catcgacacc agggtcggct
     2701 gacccgcacg gcgcgcgagc gcctctccag tttcgaacgg caaccacggt cccgccaccg
     2761 gcgggcccga acccccacgc ccgtatccgg gccagtaaag aggatgagta tcatggcgat
     2821 catcaacgga acccacaaag ccgacacgat catcggcacc gcccaggacg acagcatctt
     2881 cggcgacaac ggcaacgaca cgatcaacgg cggcgccggc aacgacacga tcgacggcgg
     2941 caacggcagc gacctgatct cgggcggcgc cggcaacgac gtcatcgacg gcggcaacgg
     3001 ccaggacacg atcgtctatg ccggcaaccg cgcggactat cgcgtgatgc agggcctgaa
     3061 cggcgcggtc atcgtccgcg acctgcgctc cggccgcccc gacggcgagg accggctgtt
     3121 cagcgccgag cgcatccagt tcgccgacgg caccttcaag ctggtcgacc tgatcgaggc
     3181 caatgccgcc ccggtcgccc ggaacgacgc cctcacgctc tccgagaccg ccggcaagac
     3241 cgaggtcact ccgaccctgc tcgccaacga cagcgacccg gacggcgatt cgctcgtcgt
     3301 ctcttcggtc caggcggttt ccgcccaggg cgcgatcgtc accctcggcc cggacggcaa
     3361 ggtcagctac gacgccggat cgatcttcgc cgacctcgac aatggccaga cggcgaccga
     3421 cagcttcacc tacacgatca ccgacggcaa cggccacgtc tcgaccgcca ccgcgacggt
     3481 gacgatcacc ggagtcaccc agaacgaggc tccggtcgcc ggcaacgacc agctgacgct
     3541 cgccgaggac gccggagcga ccgacgtcac cgcgcagctg ctcgccaacg acagcgatcc
     3601 cgacggagac gccttcaagg tcaccgaggt ccaggcggtc tccgccaagg gcgccgcggt
     3661 caccgtcgat aaggacggtg tcgtgaatta cgacgccggc aagatcttcg tctatctcga
     3721 ggaaggccag acggcgaccg acaccttcac ctacacgatc accgactcgg ccggcctcac
     3781 gtccaccgcg acggcgaccg tgacgatcac cggagtcact ccggaggtcg agatgccgga
     3841 atgggacacg tggttctacg tctccgagga cggcaccaac gacgagatgt ggggctggat
     3901 cgtcgaatcc ttcggcttcg acatcgccgg cgtcgacgcc agccacgcgc tcggcaccgt
     3961 cgagttcaac tatgagcagg gcctgctctc cttcaccgcc gaccatccca gcagcgacgt
     4021 gctcaactac gaccagcatc aggacacctg gttcacggtc atcggcgaca acggcaagaa
     4081 gatcaccgtc ggaatggtca tcgagggcgt caacgacgcc atcgtcgcgg tcgacgatca
     4141 ggttgcagtc ggcgagggcc agacgaccgg caacctctac tcgaccctcg tctcgaacga
     4201 gatcgacccg gacggcggcg cgaattcgaa ccggatcctg tcggtggaca cgaccgggac
     4261 ccagggctcg gtctcgttcg acgcgtcggg ccggacggtg atctattcgg cggcggggct
     4321 ggacctcgcc cccggcgaga cgctggtcga cagcttcacc tacctcgtcg aggacggctt
     4381 ccactcgacc gacaccgcga cggtgttcgt caccgtgacc ggcgccgccg acggcagcgt
     4441 ctcggcgtcg atgagcgact cgcttatggc ggcgttcctg ccggcaggcg gctcgggtgg
     4501 gcatgacctg ccgaccttcg acatggcgca ggcccagatg atcctcggcg ccgacgccta
     4561 catcgcctga tcccgccgag ggacagagga cggttgcgcc cacgccgtcc ccggaaggcc
     4621 gctcccgaaa gggggcggcc ttttgcgtcg caggcgcgcc gcgcgctggc gcggcagggc
     4681 gaatggagct agcccgtctc gagccaacgt ctgggcgatg gaggtggagg cgggccggct
     4741 gttcgcctac gagctgcgcc ggcccggacg cttcttccgg gtcgagttcg acctcgcccg
     4801 gccggccgcg ccgccgcccg cccggggcgc aggatagaag gggaatggca tgactgacat
     4861 tttcggctcg aacccgttcg acatcgtccc ccatctggcg gcgctggcgg tggcctatct
     4921 gcttgccttc ccgctcgggt ggaaccgcga gcgggaagag cgcagcgccg ggctgcgcac
     4981 ctttcccctg gtggcggtgg cggcgtgcgg attcgtccag gcgaccgaga ccctgacggt
     5041 gggcgagccc aacgcgatgg cgcgcatcct cgaagggatc atcgccggca tgggcttcat
     5101 cggcggcggc gcgatcctgc gccagaagga cagcgtcaag ggcacggcga ccgcggcgag
     5161 cctgtgggcg acgggcgcga tcgggacggc cgtcggcctc ggcagctacg acgtcgcgat
     5221 cgtcctgtcg ctgatcaccc tgctcacctt gtgggtccta tccccgctca agccggacct
     5281 ggtgcaggag ccggcgggcg gcgcggcggc cgggccgtcg gcgcgggagg aagaggagcg
     5341 cgcggagagc cagtcctgag gccgaagccg gacgacggac cgtaaggaaa aggggacagg
     5401 tgcggtcctg tcccccgttc ctgatcgctc ggtgggcgga ggttatttgt aggcgatcgc
     5461 ggcgctggcg gggccggcca gcggcaggat ctcgcagcgc cggaagccgg cctcgaggca
     5521 ccattcggtg aagtcggcgc cggtgaagtc gaaggcgtcg ccgaactcga tgagcatgtt
     5581 cagcgacatc agcaggccga aggcgttctg gcgccgctcg tcgtcgatga tgttctcgat
     5641 cgcgaccagc gctccgcctt cggggagcgc ccggtaggcc ttggcgatca gcatcttctt
     5701 gtgctcgaga ttccagtcgt ggaggatcat ccccatggtg atcaggtcgg cggacgggaa
     5761 ttcctccttg aggaagtcga tctcgcgcgc ggcgacccgg tcggcgaggc cggcctcgcg
     5821 caggcggcgc tcggcgatcg gcctgaccac cggcagatcg taggaggcgc aggcgaggtg
     5881 cgggtggcgg gcggcaacgg tcgccgagag ctggccggtg gcgccgccga tgtccgccat
     5941 ggtcagatag ggggcgaagt cgaacttgtc ggcgaaagcc tcgaaattgg ccatcgacac
     6001 gccccgcatc gcgtccatga actgctcgag ccgggcgggg tcggcgtaga gctcctcgaa
     6061 catcggccgc tgcgaatggc ggacctcgtt ctgcggctgg ccggtcttca gcgcgggggt
     6121 gagatcggcc cagaagcggt agaggcgggc attggccatc tcgaggatgc cgccgacgta
     6181 gcgcgggctg cagcggtcga gatagagcgc ggtcgccggc gtgttggcgt agcgcgcccc
     6241 ggcgccgtcg ccgtcgcgct ccagcatccc gacggcgacc agggtgtcga ggaaatcgta
     6301 ggtggcccgg gggtggaggc cgagcgcctg ctcgatctcg gcgccggtgc gcgactcgcc
     6361 ggcgagctcg gtgaagagtc cgagctcgac cgcgctgagc agcaccttcg aggcgaagaa
     6421 gcccattccg agctgcagga tgggagcggg ggaaaggggg gcgtgcacgg tctcttacct
     6481 cacgtgactt gggccgcgga cgcggccgga aaggcgccgc tgtgcccgag ccctgtttcc
     6541 gccgcatgtc gcgggccggc gaaaatcgtt tcaattgtca cgcccctgcg accaatcccc
     6601 gcttgcccct cggcgctgcg gcgcctatat gcgcttcacc ttcctcgaca tcgtcagacg
     6661 ttgaggctgg tcccggggac ggggccggcg cgggaaggat ttgggctttc gcccgccgtc
     6721 attgcgagga gcgaagcgac gaggcaatcc agcctcgccg cttatggatt gcttcgctgc
     6781 gctcgcaacg acgggatact gatgcaggag cgcgcgtggc ggacatcgcc gaagtgaccc
     6841 gattgatcga gccggcggct gaggccgagg ggctggcgct ggtgcgcgtc aagatgatcg
     6901 gcgggacttc ggacccgacg cttcaggtga tggcggagcg gcccgacaca aggcagctca
     6961 ccctcgagga ttgcgcccgg ctgtcgcggc ggctgtcgga gctgctcgac gaggctgatc
     7021 cgatcgagca tgcctatcgg ctcgaggtca gctcgccggg gatcgacagg ccgctgaccc
     7081 ggctcaagga cttcgacgac tggaagggcc acgaggcgcg catcgtgctc gccgagaagc
     7141 tggacgggcg caaggttttc gccggcgagc tttgcggcat cgagggcgaa acggtgctcg
     7201 tgaaggtgcc ggtccagggt ccggtcgggc tgcccttcgc cgccatccac tccgccaagc
     7261 tcgtcatgac cgacaaactc attgccgcca cggcgccgct gagcgccgag ggggccgaca
     7321 cgatcaaagt agaggaagac tgaccatggc caccaccgcc ccgggcgccc cgggcgccgt
     7381 ttccgccaac aaggccgagc tgctcgccat cgccgacgcg gtcgcccgcg agaagctgat
     7441 cgacaaggcg atcgtcatcg aggcgatgga ggacgcgatc cagcgcgccg cccgcgcccg
     7501 ctacggcgcc gagaacgaca tccgagccaa gctcgacccg gtctccgggg acctcaggct
     7561 gtggcgggtg gtcgaggttt gcgagcaggt cgacgattat ttcaagcagg tcagcctgga
     7621 ggacgctcag aagcttcagc ctggcgccgc gctgggcgac ttcatcgtcg acccgcttcc
     7681 gccgatcgag ttcggccgca tcgccgccca ggccgccaag caggtcatct tccagaaggt
     7741 ccgcgacgcg gagcgcgagc gccagtacga ggagttcaag gagcgcgccg gcgagatcat
     7801 caccggcgtg gtgaagcgcg tcgagttcgg ccacgtcgtc gtcgacctcg gccgcgcgga
     7861 gggagtcatc cggcgcgacc agcagatccc gcgcgaggtc gtccgagtcg gcgaccgggt
     7921 gcgttcgctg atcctctcgg tgcgccgcga gaaccggggg ccgcagatct ttctcagccg
     7981 cgcccatccc gacttcatga agaagctgtt cgcccaggaa gtgccggaga tctacgacgg
     8041 catcatcgag atcaaggcgg cggcgcgcga tccgggcagc cgggccaaga tcggcgtgat
     8101 cagctacgac agctcgatcg acccggtcgg cgcctgcgtc ggaatgaagg gctcgcgggt
     8161 ccaggcggtc gtccaggagc tccagggcga gaagatcgac atcatcccct ggtccgagga
     8221 cctcgccacc ttcgtcgtca acgcgctcca gccggcgacg gtcagccggg tcgtcatcga
     8281 cgaggaggag agccggatcg aggtggtggt gcccgacgac cagctcagcc tcgcgatcgg
     8341 ccgccgcggc cagaacgtcc ggctcgcctc gcagctgacc ggatcggcga tcgacatcat
     8401 gaccgaggcg gacgccagcg agaagcgcca gcgcgagttc gtcgagcgct ccgagctgtt
     8461 ccagaaggaa ttggacgtcg acgagaccct cgcccagctg ctcgtcgccg agggcttcgg
     8521 agcgctcgag gaggtcgcct atgtcgagct cgacgagctg gcctcgatcg agggcttcga
     8581 cgacgagctc gccaccgagc tccagagccg cgccgcggag gcgctggagc ggcgcgagga
     8641 agcgtcgcgc gaggagcgcc gggcgctcgg agtgtcggac gagctggccg agatgcccta
     8701 tctcaccgag cagatgctgg tcacgctggg caaggcaggg atccggaccc tcgacgacct
     8761 cgccgacctc gccaccgacg agctggtcca gaagaagcgc cccgagcagc gccgccagcg
     8821 cgagagcaac cggcccgagg acaagggcgg gatcctggcc gaatacggcc tcagcgacga
     8881 gcagggcaac gagatcatca tggccgcccg cgcccattgg ttcgaggatg aaggggagga
     8941 cgcggttgcg gaaacctccc aatgagaccc taggctttag ggtcggtcgc ggtgacgctc
     9001 ccccctccct gcacgcaggg aggggccggg ggtgggtgcg tcttcgccga ttcattgccg
     9061 gtggacaccc acccacccct aacccctccc tgtattcagg gaggggaagg tgagcgcgtc
     9121 ccacaccccc gagcgcaagt gcatcctctc cggggagcat ggctcgccgg attcgctgat
     9181 ccggctcgcc ctctcgcccg acggcgaagt gcttccagac gtgcgcgcca aggcgcccgg
     9241 gcggggggcg tggatcggcg tcgaccggcc cgcgctcgag gccgcccggg ccaagggcaa
     9301 gctgcgaggc gctctggccc gtgccttcaa gggcgctccg ctctccatcc ccgacgacct
     9361 cggcgagcgg atcgagtccg gcctgcgcca ggcggcgctc gaccggctcg gcctcgaggc
     9421 gcgcgcgggg accctggtca ccggcaccga ccgcatcgtc gacgccgcgc gcaagggcga
     9481 ggtccatctc ctcctccacg ccgccgacgc gggggaggac gggcggcgca agctcgacca
     9541 ggcctggcgg gtcggcgggc aggaggggca agggttggtt attccggctc aaaggtccat
     9601 attgtcattg gcgcttgggc ggcagaatgt ggtacatatc gccctgaagg atcgtgccgc
     9661 cgccgcgcgt gtccggcatg cgctggatcg gtggtgtgct tttatcggaa ccgaggctgg
     9721 gctagacgcc gcgacggcgc gcacgccgga cgcgtcggcc gttgatgtga acgaaggatt
     9781 gtaatgagcg agacgacaga taagccgaag cttggcgcgc gcgctcccct ggggctgaag
     9841 cgcaccgtcg agaccggcaa ggtcaagcag agcttcagcc acggccgctc caacacggtg
     9901 gtggtcgagg tcaagaagag gcgcatcctg ggccccgccg ccgcgccgga agccaaggtc
     9961 gaggaggcgc cgccgccggc cgccgcggcc gcgcgcgcgc cgcagcccgc ccagcctgcg
    10021 ccgcagcccg ccgccccgca gcgcccggtc aacgacgccg cgacccgccg cgagctgcag
    10081 gagaagctgc tccgggaagc ggaggaggcg cggctcaacg cgctcgagga ggcccgccgc
    10141 cgcgaggaca agcaggccca gcaggcgacc gaggacgaga agcgccgcgc cgaggagaat
    10201 cgccgcgccg aggaggaacg gatcgagcag gcgcgcctcg ccgccgagga agaggcccgc
    10261 cgggccacgg aggagcccgc acgtcccgcc gaagccgcac cggcgcccgc cgccgaagac
    10321 gaggacgagg accgccgtcc cgcccacgcc cggcccaagc atcacgcccc ggccgccaag
    10381 cggccggagc cggcccgccc cgcgcggggc cgcgacgacc gccgccacgc cggcaagctc
    10441 accgtcaccc gcgccctctc cgacgaggac gacagccggg cccgaagcct ggccgccctc
    10501 cgccgcgccc gcgagaagga gaagcgggcc caccagcagg cggggccgtc ggccaagcag
    10561 gtccgcgacg tcgtcgttcc cgacgcgatc accgtccagg agctcgccaa ccggatggcc
    10621 gagcgcgccg ccgacctggt caaggcgctc ttcaagatgg gcatggtggt gaccgtcaac
    10681 cagacgatcg atcaggatac cgccgagctg ctcgtcaccg agttcggcca caacatcaag
    10741 cgggtgagcg aggcggacgt cgacatccag gccgattccg acacggacgc gcccgagacc
    10801 ctgcagccgc ggccgccggt ggtcacgatc atgggccatg tcgaccacgg caagaccagc
    10861 ctgctcgacg cgatccgcgg cgccaacgtc gtctccggcg aggcgggcgg gatcacccag
    10921 catatcggcg cctatcaggt ggccctgccc gacaagtcga agatcacctt cctcgacacg
    10981 ccgggccacg aggccttcac cgagatgcgc gcgcgcggcg ccaacgtcac cgacatcgtc
    11041 gtcctggtgg tggcggccga cgacggcctc aagccgcaga cgatcgaggc gatcaaccac
    11101 accaaggcgg caggcgtgcc gatgatcgtg gcgatcaaca agatcgacaa gccgggggcc
    11161 aatgcgcaga aggtgcgcga ggagctcctc cagcacgaga tcatcgtcga ggacatgggc
    11221 ggcgaggtcc aggacgtcga ggtctcggcg ctcaagaaga ccaatctcga cggcctgctc
    11281 gaggcgatcg cgctccaggc cgagctgctc gagctgaagg ccaatccgga ccggtccgcc
    11341 gagggggcgg tggtcgaggc caagctcgac aagggccgcg gcccgctcgc caccgtgctc
    11401 atccagcgcg ggacgctcaa ggtcggcgac gtgttcgtgg tcggaccggt gccgggcaag
    11461 gtccgcgcga tgatcgacga caagggcaag caggtgaagg aggccggccc ctcggtcccg
    11521 gtcgaggtgc tcggcctgtc cggcgtcccg tccgcgggcg acacgctcac cgtggtcgag
    11581 aacgaggccc gcgcccgcga ggtcgccgcc tatcggcaga gcgtgctcga ccgcaagcgc
    11641 accaccagcg ctccggtcag cgtcgagcac atgttcgcgt cgaaggcgag ccaggccaag
    11701 gaattcccgc tggtcgtcaa ggccgacgtg cagggctcgg ccgaagcgat cgtcaacgcg
    11761 gtcaaccgaa tctcgaccga ggacatcaag gctcggatcc tcaactccgg agtcggagcg
    11821 atcaccgaga gcgacgtcat cctcgcggcg gcgagcggag cgcccatcat cggcttcaac
    11881 gtccgcccga acgccaaggc gcgcgaggcg gctgagcgca acaaggtcga gttccgctat
    11941 tacgacgtga tctatcacct caccgactgg gtgcgcgaag cgatgggcgg ccagctgggg
    12001 ccggagatc
//
LOCUS       DASVQZ010000244         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_338585, whole genome shotgun sequence.
ACCESSION   DASVQZ010000244 DASVQZ010000000
VERSION     DASVQZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338585"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF547_06620"
     CDS             <1..494
                     /locus_tag="VF547_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX8262529.1"
                     /translation="RLEHGLSPWVAFLIVPLFGFANAGVSLAGVGADRLLAPLPLAIA
                     AGLFLGKQIGILGAVWLAVRLGLASRLRGATWLQVWGIAMLCGIGFTMSLFIGALAFP
                     GDAGRVEEAKLGILAGSFLSALGGYAVLRLAPGHASHQEEEAMQDCEIGNDGDVEGLE
                     ERR"
     gene            491..1777
                     /locus_tag="VF547_06625"
     CDS             491..1777
                     /locus_tag="VF547_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262530.1"
                     /translation="MTRWLLLLAALLLAGCASTGPARQSLDAIARDYVKLQLAIGEKE
                     EGYIDAYYGPPEWQAEAKLKPQSLPELAARAGVLAVRLRSLADARLQPIERRRRDFLL
                     ANLRAASTRLRMIGGEKLPFADEAEGLFGVRPQLRPLSAYDPVLARIERLLPGTGALA
                     DRVDAFQERYTIARDRLEPVMRSAIAECRRRTLAHIPLPANERFTLEFVTGKSWGGYN
                     WYKGDSNSVIQVNTDLPVRIGRAVDLGCHEGYPGHHVHNLLLEEKLAKGRGWVEFSLY
                     PLYSPQSFIAEGSANYGIELAFPGTEQLAFETATLYPIAGLPTEAAGRYLALQEAMRD
                     LAGARFTIAADYLDGRIDRARAVELIRKYQLVSPARAEKSLAFIDQYRSYVINYGLGL
                     DHVRRAVEGSGASPAERWAAMARILSEPTLPSDLLR"
     gene            1777..2784
                     /locus_tag="VF547_06630"
     CDS             1777..2784
                     /locus_tag="VF547_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX8262531.1"
                     /translation="MRLFWDERQRLHAPAQELHNGGFVPYAEHCGRIDSMLAALGACS
                     GSADHGEAPLLRVHPRDYLDFLRTAFADWRSAGRPGDASGYVWPVVGRRPLDLDRIDA
                     RLGRYSMDASTPIAEGTWESAYWSAQTALSALDAVLGGERSAFALCRPPGHHAGADYM
                     GGYCYLNNAAIAAEAALAAGRARVAVLDVDYHHGNGTQDLFLGRGDVFFASLHADPRT
                     DFPFFWGHADETGTGEGAGATLNLPLPRGTGRPGYEAALDRALAAVAGFGAELLVCSF
                     GADTFSGDPICSFALETADYAPVARRIASLGLPTLVVMEGGYAVDALGANVASFLEGL
                     Y"
     gene            2799..3356
                     /locus_tag="VF547_06635"
     CDS             2799..3356
                     /locus_tag="VF547_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8262532.1"
                     /translation="MKIRLLLACLLAVACAQEGAAKGVLAADRPETQASATFTVTSRD
                     FKAGGEIPKRFTDYDIGMSPSLVWKGLPEGTRSLVVIMEDPDASSSQPFVHWLAWNID
                     PAAGAVDRGSVTLGARLGRNGRGHPAYFGPRPPGKAAHHYHFQMFALDSQLTLKTGAS
                     REQLLQAMRGHVLAKASLVGTFAKP"
     gene            complement(3610..>4474)
                     /locus_tag="VF547_06640"
     CDS             complement(3610..>4474)
                     /locus_tag="VF547_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX8262533.1"
                     /translation="TAASKLGEVHLLVAGEGVGAVAEAAARIAGVGKVHVADGAAYAH
                     QLAENVAPLAAQLMQGHDAFVAASTTTGKNVAPRVAALLDVMQISDVIGIEGEDTFQR
                     PIYAGNAIATVKSSDSKKVLTVRGTAFEKAAAEGGSGQVEAVSGGGDAGLSSFVGAEI
                     AASERPELTSAKIIVSGGRALGSSEQFHALIDPLADKLGAAVGASRAAVDAGYAPNDY
                     QVGQTGKIVAPEVYIAVGISGAIQHLAGMKDSKLIVAINKDEEAPIFQVSDLGLVGDL
                     FKLVPELTGKL"
BASE COUNT          616 a         1648 c         1488 g          722 t
ORIGIN      
        1 accggctgga gcacggcctg agcccctggg tcgccttcct gatcgtcccc ttgttcggct
       61 tcgccaatgc cggggtctca ttggccgggg tgggggcgga ccggctgctg gcgccgctgc
      121 cgctcgcgat cgcggcgggc ctgttcctcg gcaagcagat cggaatcctc ggcgcggtct
      181 ggctggccgt acgcctcggc ctcgcctccc gccttcgcgg cgccacctgg cttcaggtct
      241 ggggaatcgc catgctctgc ggcatcggct tcaccatgag cctgttcatc ggcgccctcg
      301 cctttcccgg cgatgcaggc cgggtcgagg aagcgaagct cggcatcctc gccggctcct
      361 tcctctcggc cctgggcggc tacgccgtgc ttcggcttgc gcccggccac gccagccatc
      421 aggaggagga ggccatgcag gattgcgaga tcgggaacga cggcgacgtc gaagggctgg
      481 aggagcgcag gtgacgagat ggctgctcct gctcgccgcg ctcctgctcg ccggctgcgc
      541 ctcgaccggc ccggcgcggc agagcctcga cgccatcgcc cgcgactatg tgaagctcca
      601 gctcgccatc ggcgagaagg aggagggcta tatcgacgcc tattacggcc cgcccgaatg
      661 gcaggctgag gcgaagctga agccgcagtc cttgccggag ctggccgcgc gcgccggagt
      721 cctcgccgtt cggctgcgca gcctcgccga cgcccggctc cagccgatcg agcgccggcg
      781 ccgcgacttc ctcctcgcca atctccgcgc cgcctcgacc cgcctcagga tgatcggggg
      841 cgagaagctt cccttcgccg acgaggcgga ggggctgttc ggggtccggc cgcagctcag
      901 gccgctctcc gcctacgatc cggtgctcgc ccggatcgag cggctgttgc cgggcaccgg
      961 cgcgctcgcc gaccgggtgg acgccttcca ggagcgctac accatagcgc gggaccggct
     1021 cgagccggtg atgcgctccg ccatcgccga atgccgccgc cggacgctcg cccatatccc
     1081 gctgccggcg aacgagcggt tcaccctgga gttcgtcacc ggcaagagct ggggcggcta
     1141 caattggtac aagggcgatt ccaacagcgt catccaggtc aataccgatc tgccggtccg
     1201 catcggccgc gccgtcgacc tgggctgcca cgaaggctat ccgggccacc acgtccacaa
     1261 cctgctcctc gaggagaagc tggcgaaggg acgcggctgg gtcgagttca gcctctaccc
     1321 gctctattcg ccgcaaagct tcatcgccga gggctcggcc aattacggca tcgagctcgc
     1381 cttccccggc acggagcagc tcgccttcga gaccgccacc ctctatccga tcgccggact
     1441 gccgaccgag gccgccggcc ggtacctcgc cttgcaggag gcgatgcgcg acctggccgg
     1501 agcccgcttc acgatcgcgg ccgattatct cgacggccgg atcgatcgtg ccagagcggt
     1561 cgagctgatc cgcaaatatc agctggtctc ccccgcgcgg gcggaaaagt cgctcgcctt
     1621 catcgaccag taccgctcct acgtgatcaa ttacggcctc ggcctcgacc atgtccgccg
     1681 ggcggtcgag ggctccggcg cctcgccggc ggagcgctgg gcggcgatgg cccggatatt
     1741 gtccgagccg accctgccgt cggacctcct tcgctgatgc gcctcttctg ggacgagcgg
     1801 cagcggctcc acgcgcctgc gcaggagctg cacaacggcg gcttcgtgcc ctatgcggag
     1861 cattgcgggc ggatcgattc gatgctggcc gcgctgggcg cctgcagcgg ctccgccgac
     1921 catggcgagg cgccgctgct gcgggtgcat ccccgcgact atctcgactt cctgaggacc
     1981 gcgttcgcgg actggcggtc ggccggacgc ccgggcgacg cttcgggcta tgtctggccg
     2041 gtcgtcggcc ggcgcccgct cgacctcgac cggatcgacg cccgcctcgg ccgctacagc
     2101 atggacgcct cgaccccgat cgcggagggg acctgggaga gcgcctattg gtcggcccag
     2161 accgcgctct ccgcgctcga cgcggtgctc ggtggcgagc gctccgcctt cgccttgtgc
     2221 cggccgcccg gccaccatgc cggcgccgac tatatgggcg gctattgcta cctcaacaac
     2281 gccgcgatcg ccgccgaggc ggccctcgcc gccggccggg cccgagtcgc agtcctcgac
     2341 gtcgactatc atcatggcaa cggcacccag gacctgttcc tcggccgcgg cgacgtcttc
     2401 ttcgcctcgc tccacgccga tccgcgaacc gatttcccct tcttctgggg ccatgccgac
     2461 gagaccggga ccggcgaagg ggctggggcg accctcaatc tgccgctgcc ccgcggcacc
     2521 ggccggcccg gctatgaggc ggcgctcgat cgggccctcg ccgcggtggc gggcttcggc
     2581 gccgagctgc tggtctgctc gttcggcgcc gacacctttt ccggcgaccc gatctgctcg
     2641 ttcgcgctcg agaccgccga ttacgccccc gtggcccgcc gcatcgcctc gctcggcctg
     2701 ccgaccctgg tcgtgatgga gggcggctac gcggtcgacg cgctcggggc caatgtcgcc
     2761 tctttcctgg agggcctgta ttaagttacg gtggcggcat gaagattcgc ctgctcctcg
     2821 cctgtcttct cgccgtcgcc tgcgcgcagg aaggcgccgc caagggcgtc ctcgccgccg
     2881 accggcccga aacccaggcc tccgccacct tcaccgtcac ctcgcgcgac ttcaaggcgg
     2941 gcggcgagat ccccaagcgc ttcaccgatt acgacatcgg catgtccccc tcgctggtct
     3001 ggaagggcct gcccgagggg accaggagcc tcgtcgtgat catggaggat cccgacgcct
     3061 cctcgtccca gcccttcgtc cactggctgg cctggaacat cgacccggcg gccggcgcgg
     3121 tcgaccgcgg ctcggtgacg ctcggcgcca ggctgggccg caacggccgc ggccatcccg
     3181 cctatttcgg cccccgcccg ccgggcaagg ccgcgcatca ttatcatttc cagatgttcg
     3241 cgctcgactc gcagctcacc ctcaagacgg gcgcgagccg cgagcagctg ctccaggcga
     3301 tgcgcggcca cgtcctcgcc aaggcgagcc tggtggggac cttcgccaag ccctgaatcc
     3361 tccccctcgc gagacgggga gggggccgcc cgaagtgcgg tggaggggtc cttcgatacg
     3421 ccgcttcgac ttcgctcagc ggctactcag gatgagcggg aaagaagcgc ccatccttct
     3481 ctacgccgct tcgacttcgc tagcggctac tcaggatgag cgggaaagaa accgctcatc
     3541 ctgagtaggg actgagcctg gcgaaggccc gtatcgaagg accctccacc atgctgagca
     3601 tggggaggat tacaatttcc cggtcagctc cggcacgagc ttgaacaggt cgccgacgag
     3661 gccgaggtcg gagacctgga agatcggagc ctcctcgtcc ttgttgatgg cgacgatcaa
     3721 cttggaatcc ttcatccccg cgaggtgctg gatcgcgccg gagattccga ccgcgatata
     3781 gacttcgggg gcgacgatct tgccggtctg gccgacctga tagtcgttgg gcgcgtagcc
     3841 cgcgtcgacc gcggcgcggc tggccccgac cgcggctccg agcttgtcgg cgagcgggtc
     3901 gatcagcgcg tggaactgct cggacgagcc gagcgcccgg ccgccggaga cgatgatctt
     3961 ggcgctggtc agctcgggac gctcggaggc cgcgatctcg gcgccgacga agctcgagag
     4021 gcccgcgtcg ccgccgccgg acaccgcctc gacctggccg gatccgcctt cggccgcggc
     4081 cttctcgaac gcggtgccgc gcaccgtcag caccttcttc gagtccgacg acttcacggt
     4141 cgcgatcgcg ttgccggcgt agatcggacg ctggaaggtg tcctcgccct cgatcccgat
     4201 cacgtccgaa atctgcatca cgtcgagcag cgcggcgacc ctcggcgcga cgttcttgcc
     4261 ggtcgtcgtg gaagcggcga cgaaggcgtc gtggccctgc atcagctggg cggcgagcgg
     4321 cgccacgttc tccgccagct ggtgggcgta ggcggcgccg tcggcgacat ggaccttgcc
     4381 gacgcccgcg atcctcgccg ccgcctcggc gactgcgccg accccttcgc cggcgaccag
     4441 cagatgcacc tcgcccagct tcgaggccgc ggtc
//
LOCUS       DASVQZ010000245         8194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_819401, whole genome shotgun sequence.
ACCESSION   DASVQZ010000245 DASVQZ010000000
VERSION     DASVQZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819401"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4121
                     /locus_tag="VF547_06645"
     CDS             <1..4121
                     /locus_tag="VF547_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX8262534.1"
                     /translation="AIGLFFVWLNSSLGHRYVVKQINELETASGLDVDVGRIEGSLFG
                     ELIIHDIRLKDPKGTFFAARRATVDWRPLTYFRNHIDIKRLEIPVARLYRLPQLKPGD
                     PNAPLLPDIDLDIGKIEIGRILVDPPVTGYRHLLSLSGSARIDKGRAQVALDSNAIAA
                     PGLPGGDKLALRLDAVPEENRLAMGVRVEAPANGFVAKLTGLNQPLVASVGGNGTWTN
                     WKGRARAQLGGKSFADLAVGGRDGTFTVTGPVNPGMLMAAGPAKNLLSPLVQVNLETK
                     LDQRRADLKLRLNSAAMAIVADGLVDLGRNQFQDFRVAARLSRPRAIGPNIDGTDVRV
                     AMVLNGAFATPFVAYDLRARSLSFNETTIELLQARGRAKVDADRISIPIQARARRISG
                     LDPSLGSLLTNVAANGTINISGTRLVSDNLKIRSDRLNATVALAGDFAKGQYVAGIQG
                     TVNNYHMEGIGLLDLTTDMDVFTAANGFGLKGRVAVRTRRIDNSTAAGLLEGNATAAA
                     NVTMTPAGVITLDNVRLSSPGLRITSGGGTIWPDGRISIRAAGVSRAYGAIAVVVTGT
                     ATRPQIALKAASPGFGIGLRDVSATVRAVAGGYAINATGQSAYGPFEADVTILSGRGP
                     LTIDIRRLLFAGMTFAGRVTRTAAGPFVGTLAMTGEGLQGTVRLAAQGRYQRADIDAT
                     AAGARIPGENPILIQRGLVRATVILYPDAPHIVGDAQLAGVRASNFFLARARAKVDYR
                     GGNGTAQLFAEGTSGVPFRVAINSVLTPDHIRAAAQGQVRNIPFRLARPAEISKVAGG
                     WQLAPTELVLPQGNVQLAGRYGNGLVVQSRLRDFDISILNVFSPGLGLGGRATGSIDF
                     AQVGGDSFPRADARLTVAGFTRTGVAVRSPPVDLVLAGQLRPEGGSLASVIRRAGNVI
                     GRAQFRLQPVGPGAGSWMTRLLAAPLGGGIRYNGPAEVLWALTGIADMQLSGPIGVAA
                     DFTGRVQSPQLTGIVRANDLTFVHETYGTRISQLAIQGRFTSSELQITQLSGRAGRGT
                     IAGTGTVGFASAAGYPMDIRLKLDDAQLARSDNIGATLTGDLAITNNRRDGALISGDL
                     SLPEVRYQIIRQGAAQVVELAGVRRKGEPLPTVRDATQQADAAAPSIWKLDLRLRADN
                     RLFIAGMGLESEWSADLRVQGTTATPTLVGEADLIRGTYSFSGQRFDVTRGHIAFTGQ
                     RPPNPRIDIVASADVKDVTVNIVVSGSANNPQIAFTSSPALPQDEIMARILFGGSVTE
                     ISAIQAIQLASSLNSLRGGGGGLNPLGKLRSAGGFDRLRILGADDTTGRGTAIAAGFY
                     LSDDIYLEIITDARGFTATQIEIALSKALSILSQVGTSGGGNNVNVRYRKQY"
     gene            complement(4259..5341)
                     /locus_tag="VF547_06650"
     CDS             complement(4259..5341)
                     /locus_tag="VF547_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8262535.1"
                     /translation="MRRAAALIVGGGPGGSAAAIGLARAGIEAELIERSEGPHDVVCG
                     GFLGWDALAALSRLGLDPQALGARPIGRLRLISAKRSLEVALPSMAAGLSRRTLDEAL
                     LGAAAEAGARVARGRAVRAADCRRLQVRLESGEEVAAGALVLATGKHELRGAPRPVDI
                     SARPLGLRTALVPDRALARSLDGIIELHLYDGGYAGLLMQEDGTANLCLSASRKRLKE
                     AGGIEKLVERLADELPALAERLAQGEASGWSAVSGIPYGWRATATAAGVYRVGDQAAV
                     IASLAGDGIAIALESGTAAARAIACGRPAGDFQREWARRARRPVGLAELLRRSAEHAT
                     SRDAMMGLLGWFPSLAPLAAKLTRIG"
     gene            complement(5320..6009)
                     /locus_tag="VF547_06655"
     CDS             complement(5320..6009)
                     /locus_tag="VF547_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8262536.1"
                     /translation="MRSLKSRVRDDEQMDDPSLDPQVYDEVLKGLGRVNAWTFAARPT
                     LAFLERATRGRTRFSLLDVGSGEGGMLRVIAKWAGKRGIRAELTGIDLNPKSQAVARA
                     MTPPGTAIDYRTGDYRDLGRFDLVVSSLVAHHMTDGQLLDFLRWMESNSDRGWFVNDL
                     HRHAFAYYGFPLLTTLLRVHRIVREDGQLSIARSFRPGDWRAILAEAGVAEAAEVRRY
                     FPFRLCVERLR"
     gene            complement(6006..7103)
                     /locus_tag="VF547_06660"
     CDS             complement(6006..7103)
                     /locus_tag="VF547_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX8262537.1"
                     /translation="MQFRAVAKGSRTSQAHLNAVASAVPAHDVHDAFIRWAQARLGEP
                     RLRALFARMAARSGIEHRWSVLPAAPYGGSPVDPGGFYGGKLPSTDVRMQVYAQAAPK
                     LALEAIRRLDAAALVGITHLIVASCTGFVAPGIDQIIAAELGLGGHVERTLVGFMGCY
                     AAVSSLRLAHHIVRSEPEARVLVVTVELSSLHLQYEEDVERLLAALQFGDGATASLVS
                     ADPSGFALSRPFAATLADSSALIRWRIGDHGFVMDLSGEVPGRIGAALRDRGVREAML
                     GNEAPAAFDAWAVHAGGRSVLDAVEHGLDLRPEALGESREVLARFGNMSSSTLPFVLE
                     RLLANPQRRRGIALAFGPGLAAEGFHFERAA"
     gene            complement(7163..7238)
                     /locus_tag="VF547_06665"
     tRNA            complement(7163..7238)
                     /locus_tag="VF547_06665"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7203..7205),aa:Phe,seq:gaa)
     gene            complement(7278..7472)
                     /gene="yacG"
                     /locus_tag="VF547_06670"
     CDS             complement(7278..7472)
                     /gene="yacG"
                     /locus_tag="VF547_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX8262538.1"
                     /translation="MSTSSVPRSGKGCPLCGKPASAEHAPFCSRGCRDRDLLNWLGDS
                     YRVPGPPAVEIEEGGLDSER"
     gene            complement(7450..>8194)
                     /locus_tag="VF547_06675"
     CDS             complement(7450..>8194)
                     /locus_tag="VF547_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEX8262539.1"
                     /translation="KVRVEIVREAIAEPGHPKFAKGRITDAEEKEGPSLGERIGSPRP
                     LPRLGPDLFYEAGWSELIDEALGTPIDFAGGELRMSLTPAMTLFDVDGFLPPFELALA
                     AAEEAAYAIRRLDIGGSIGIDLPTVASRDERQRVVAALDAVLPRPFERTAVNGFGFLQ
                     VVRRRERRSLPELIQWDEAGAAARDLLRTAERAVGGCELAASPDVTARLRAEPGWLDA
                     LARRIGGPVALREEPGFTTWRSHVHVKRS"
BASE COUNT         1250 a         3095 c         2746 g         1103 t
ORIGIN      
        1 ttgcgatcgg cctgttcttc gtctggctga attcctcgct cggccaccgc tatgtggtca
       61 agcagatcaa cgagctcgag acggcctccg ggctcgacgt cgacgttggg cggatcgaag
      121 ggtccctgtt cggcgagctg atcatccacg acatcaggct caaggacccg aaggggacct
      181 tcttcgcggc ccggcgggcc acggtcgact ggcggccgct cacctatttc cgcaaccata
      241 tcgacatcaa gcggctcgag atcccggtcg cgcgcctcta ccgcctgccg cagctcaagc
      301 ccggcgatcc caacgcgccg ctccttcccg acatcgacct cgacatcggc aagatcgaga
      361 tcgggcgaat cctcgtcgat ccgcccgtca ccggctaccg ccacctgctc agcctctccg
      421 gcagcgcccg gatcgacaag ggccgcgccc aggtcgcgct cgacagcaac gcgatcgccg
      481 cgcccggcct tccgggcggc gacaagctcg ccctccggct ggacgcggtg ccggaggaga
      541 accgcctggc catgggggtg cgggtcgagg cgccggcgaa cggcttcgtc gccaagctga
      601 ccggcctgaa ccagccgctc gtcgccagcg tcggcggcaa cggaacctgg accaactgga
      661 agggacgcgc gcgcgcccag ctcggcggca agtcgttcgc cgacctcgcc gtcggcggcc
      721 gcgacggcac gttcaccgtg acgggtcccg tcaatccggg catgctgatg gcggccgggc
      781 cggcgaagaa cctgctctcg ccgctcgtcc aggtgaacct cgagacgaag ctcgaccagc
      841 gccgagccga cctgaagctc cggctcaatt cggcggccat ggcgatcgtc gcggacggcc
      901 tcgtcgacct cggccggaac cagttccagg acttccgggt cgccgcgcga ctgtcgcgcc
      961 cgagggcgat cggcccgaat atcgacggca ccgacgtccg ggtggcgatg gttctcaacg
     1021 gcgccttcgc cacccccttc gtcgcctacg accttcgcgc ccgctcgctg agcttcaacg
     1081 agacgacgat cgagctgctc caggcgcgcg gccgggccaa ggtcgacgcc gaccggatca
     1141 gcatcccgat ccaggcccgc gcgcggcgga tcagcggcct cgatccctcg ctcggcagcc
     1201 tgctcaccaa cgtggcggcg aacggcacca tcaacatcag cggaacccgg ctcgtttccg
     1261 acaacctcaa gatccgctcg gaccggctca acgccaccgt cgcgctggcc ggcgacttcg
     1321 ccaagggcca gtacgtcgcg ggcatccagg gaacggtcaa caactaccat atggagggga
     1381 tcggcctcct cgacctcacc accgacatgg acgtgttcac cgccgccaac ggcttcggcc
     1441 tgaagggccg ggtcgcggtg cgcacccgcc gaatcgacaa cagcaccgcc gccggcctgc
     1501 tcgagggcaa tgccaccgcc gccgccaacg tgaccatgac tccggccggc gtgatcaccc
     1561 tcgacaatgt ccgcctgagc tcgcccggcc tgcgcatcac ctccggcggc ggcacgatct
     1621 ggccggacgg gcggatcagc atccgcgccg ccggcgtctc gcgggcctat ggggcgatcg
     1681 ccgtcgtcgt gaccgggacg gcgacccggc cgcagatcgc gctcaaggcg gcgagcccgg
     1741 gcttcggaat cggccttcgg gacgtcagcg cgacggtgcg cgccgtcgcc ggcggctatg
     1801 cgatcaacgc caccggccag tccgcctacg gcccgttcga ggcggacgtc acgatcctgt
     1861 ccgggcgcgg tccgctgacc atcgacattc gccgactgct cttcgccggc atgaccttcg
     1921 ccgggcgggt gacccggacg gccgcgggtc cgttcgttgg gacgctcgcc atgaccggcg
     1981 aggggcttca gggcacggtc cggctcgccg cccagggccg ctaccagcgc gccgatatcg
     2041 acgcgaccgc cgccggcgcc cggattccgg gcgagaatcc gatcctgatc cagcgcgggc
     2101 tcgtccgggc gaccgtgatc ctctatccgg acgcgccgca catcgtcggc gatgcccagc
     2161 tcgccggagt tcgagccagc aacttcttcc tcgcccgggc gcgggcgaag gtcgactatc
     2221 gcggcggcaa cggcaccgcc cagctgttcg cggaaggcac cagtggagtg ccgttccggg
     2281 tcgcgatcaa ttcggtgctg acgcccgacc atatccgcgc cgccgctcag gggcaggtgc
     2341 gcaacatccc gttccggctg gcccgcccgg ccgagatcag caaggtcgcc ggtggctggc
     2401 agctggcccc gaccgagctc gtattgcccc agggcaacgt ccagctggcc ggacgctacg
     2461 gcaacggcct ggtcgtccag tcgcgcctca gggatttcga catctcgatc ctcaacgtct
     2521 tctcgccagg cctcggcctc ggtggccgcg ccacgggcag catcgacttc gcccaggtcg
     2581 gcggcgacag cttcccccgg gccgacgccc gactcaccgt cgccggcttc acccgcaccg
     2641 gagtcgccgt gcgctcgccg ccggtcgatc tcgtcctcgc cggccagctg cgcccggaag
     2701 ggggttcgct cgcctcggtg atccgccgcg cgggcaacgt catcggccgc gcccaattcc
     2761 ggctgcagcc ggtcgggccg ggcgccggca gctggatgac ccgcctgctc gccgctccgc
     2821 tcggcggagg catccgctat aacggccccg ccgaggtcct gtgggcgctg accggcattg
     2881 ccgacatgca gctttccggc ccgatcggag tggccgccga cttcacgggc cgggtccaga
     2941 gcccgcagct caccggcatc gtccgggcca acgacctaac cttcgtccac gaaacctacg
     3001 gcacccggat cagccagctt gcgatccagg gccgcttcac cagctccgag ctgcagatca
     3061 cgcagctgtc cggccgcgcc ggccgcggca ccatcgccgg caccggaacg gtcggcttcg
     3121 cctccgccgc cggctatccg atggacatcc ggctgaagct cgacgacgcc cagctcgccc
     3181 gaagcgacaa tatcggcgcc accctgaccg gcgatcttgc catcaccaac aaccgccgcg
     3241 acggcgccct catctccggc gacctgagcc tgcccgaggt gcgctaccag atcatccgcc
     3301 agggcgccgc gcaggtggtc gagttggccg gcgtccgccg caagggcgag cctctgccca
     3361 ccgtcaggga cgcgacccag caggccgacg ccgccgcacc cagcatctgg aagctcgacc
     3421 tccgcctgcg cgccgacaac cgcctgttca tcgccgggat gggcctcgaa tccgaatgga
     3481 gcgccgacct gcgcgtccag ggcacgaccg ccaccccgac cctggtcggc gaagcggacc
     3541 tgatccgcgg cacctacagc ttctccggcc agcgtttcga cgtcacccgc ggccacatcg
     3601 ccttcaccgg ccagcggccg cccaatccgc gcatcgacat cgtcgccagc gccgacgtga
     3661 aggacgtcac cgtcaacatc gtcgtcagcg gcagcgccaa caacccgcag atcgccttca
     3721 cctcctcccc ggcgctgccg caggacgaga tcatggcgcg gatattgttc ggcggttcgg
     3781 tgaccgaaat ctccgcgatc caggcgatcc agctggcctc gtcgctcaac tcgctccgcg
     3841 gcggcggcgg cggcctcaat ccgctcggga agctgcgctc cgccggcggc ttcgaccggc
     3901 tgcgcatcct cggcgccgac gacacgaccg gccgcggcac cgccatcgcc gcgggcttct
     3961 acctgtccga cgacatctat ctcgagatca tcaccgacgc ccgcggcttc accgccaccc
     4021 agatcgagat cgccctctcg aaggcgctga gcatcctctc ccaagtcggc acctccggcg
     4081 gcggcaacaa cgtcaacgtg agataccgca agcaatatta gcctgaattc ctcccccgga
     4141 tttccggggg agggggaccg cgcgaagcgc ggtggagggg ttctacggac tccccaagaa
     4201 cccctccacc aggcttcgcc tggtccccct ccccggcaaa tgccggggag gattgcgcct
     4261 agccgatcct ggtcagcttc gcggccagcg gggcgagcga cgggaaccag ccgagcagcc
     4321 ccatcatcgc atcgcgggac gttgcgtgct cggccgagcg tctcagaagc tcggcgagcc
     4381 cgaccggccg gcgggctcga cgggcccatt cccgctggaa atccccggcc ggcctgccgc
     4441 acgcgatcgc ccgcgccgcg gccgtcccgc tctccagcgc gatggcgatg ccgtcgccgg
     4501 cgagcgacgc gatgaccgcg gcctggtcgc ccacccgata gactcccgcg gccgtcgccg
     4561 tggcccgcca gccgtagggg attccggaga ccgcgctcca gcccgacgcc tcgccctggg
     4621 ccagccgctc agccagagcc ggaagctcgt cggccagccg ctccaccagc ttctcgatgc
     4681 cgccggcctc cttcaggcgc ttgcgcgagg cggagaggca cagattggcc gtgccgtcct
     4741 cctgcatcag caagccggca tagccgccgt cgtagaggtg gagctcgatg atcccgtcca
     4801 gcgaccgcgc gagcgcccgg tccgggacga gcgcggtgcg aagcccgagc ggccttgccg
     4861 agatgtccac gggccggggg gcgccgcgaa gctcgtgctt gcctgtggca aggaccaggg
     4921 ccccggcggc cacctcctcc ccgctttcga gccgcacctg gagccgccgg caatccgccg
     4981 cccgaaccgc ccgcccccgc gccactctcg ctccggcctc ggcggcggcg ccgagcagcg
     5041 cctcgtccag ggtgcgccgg gagaggccgg ccgccatgct cggcaaggcg acctccaggc
     5101 tccgcttcgc cgagatcagc cgaagccgtc cgatcgggcg cgcgccgagc gcttgcgggt
     5161 cgagccccag cctcgacagg gccgccagcg cgtcccagcc gaggaagccg ccgcacacga
     5221 catcgtgcgg cccctcgctg cgctcgatca gctcggcctc gatgccggcg cgcgccaggc
     5281 cgatcgccgc ggcggatccg ccggggccgc cgccgacgat cagcgcagcc gctcgacgca
     5341 cagccggaac gggaaatagc gccgcacctc cgccgcttcc gcgacccccg cctcggcgag
     5401 gatcgcccgc cagtcgccgg ggcggaacga cctggcgatg gagagctggc cgtcctcgcg
     5461 gacgatccga tggacccgca gcagggtggt gagaagcgga aagccgtaat aggcgaaggc
     5521 gtggcgatgg aggtcgttga cgaaccatcc gcgatcggaa ttcgactcca tccagcgcag
     5581 gaagtcgagc agctgtccgt cggtcatgtg atgcgcgacc aggctgctca ccacgaggtc
     5641 gaaacggccg agatcgcgat agtcgccggt gcggtagtcg atcgccgttc cgggcggagt
     5701 catggcccgg gccaccgctt ggctcttcgg gttgaggtcg atcccggtca gctccgcgcg
     5761 gatgccgcgc tttcccgccc acttggcgat cactcgaagc attccgcctt cgcccgagcc
     5821 gacgtcgagc aggctgaacc gcgtccggcc gcgggtcgcg cgctcgagga aggccagcgt
     5881 cggccgcgcc gcgaaggtcc aggcattgac ccggcccagg cccttgagca cctcgtcata
     5941 gacttgggga tcgagcgacg gatcgtccat ctgctcgtcg tcgcggaccc ggctcttcag
     6001 cgatctcatg ccgcccgttc gaagtggaac ccttccgcgg ccaggccggg cccgaaggcg
     6061 agagcgatgc cgcgccgccg ctgcgggttc gccagcagcc gttcgagcac gaacggcagg
     6121 gtggacgagg acatgttgcc gaagcgggcg aggacctcgc gcgactcccc cagcgcctcc
     6181 ggccgcaggt cgaggccgtg ctcgaccgcg tcgaggaccg agcggccacc ggcgtggacg
     6241 gcccaggcgt cgaacgccgc cggcgcctcg ttgccgagca tcgcctcgcg cacgccgcgg
     6301 tcgcgaagcg ccgcgccgat ccggcccggc acttctcccg acaggtccat gacgaagccg
     6361 tggtcgccga tgcgccagcg gatcagggcg ctcgaatcgg ccagggtcgc cgcgaacggc
     6421 cgggagaggg cgaagccgct cgggtcggcg ctgaccagcg aggccgtggc gccgtcgccg
     6481 aactgcagcg ccgcgagcag ccgctcgaca tcctcctcat attggaggtg gaggctggaa
     6541 agctcgacgg tcaccaccag cacccgcgcc tccggctcgg agcggacgat gtggtgggcg
     6601 agccggagcg acgaaaccgc ggcgtagcag cccatgaagc cgaccagggt ccgctcgaca
     6661 tggccgccga ggcccagctc ggcggcgatg atctggtcga tacccggcgc gacgaagccg
     6721 gtgcagctgg ccacgatcag gtgggtgatc ccgaccagcg ccgccgcgtc gagccgccgg
     6781 atcgcctcaa gggcgagctt tggagccgcc tgcgcataga cctgcatccg gacgtccgtg
     6841 ctcggcagct ttccgccgta gaagccgccc ggatcgaccg gcgagccgcc gtagggcgcc
     6901 gccggaagca ccgaccagcg atgctcgatc cccgatcgcg ccgccatccg ggcgaacaga
     6961 gcgcgcagcc ggggctcgcc cagccgcgcc tgcgcccagc ggatgaacgc gtcgtggacg
     7021 tcgtgcgcgg gcacggcgct ggccaccgcg ttgagatggg cctggctcgt acgactcccc
     7081 ttggcaacag cgcggaattg cagcccagat tagatgaacg aaggctgagg aagttcccga
     7141 ccgccaccgg atggggaagg gctggtgccc aggggcggaa tcgaaccacc gacaccgtga
     7201 ttttcagtca cgtgctctac caactgagct acctgggcgt tccggcggaa gccgaagacg
     7261 agcgcctata gaggcgctca gcgctcactg tccagcccgc cctcctcgat ttccacggcg
     7321 ggaggaccgg ggacgcgata cgagtcgccg agccagttga gaaggtcgcg gtcgcggcag
     7381 ccgcggctgc agaagggcgc gtgctcggcg ctcgcgggct tgccgcacaa ggggcagccc
     7441 ttgccgcttc taggaacgct tgacgtggac atggctgcgc catgtggtga agccgggctc
     7501 ctcgcgcaag gcgaccgggc cgccgatgcg ccgggcgagc gcgtcgagcc agccgggctc
     7561 ggcccgaagc cgtgcggtca cgtcgggcga ggccgcgagc tcgcagcctc cgacggcgcg
     7621 ctccgccgtg cgcagcaggt cgcgggcggc ggcgccggct tcatcccact ggatcagctc
     7681 gggcagcgag cgccgctcgc gccgccgcac cacctggagg aagccgaagc cgttgaccgc
     7741 ggtccgctcg aacggccgcg gcagcaccgc gtcgagcgcc gcgacgacgc gctgccgctc
     7801 gtcgcgcgag gcgacggtcg gcaggtcgat cccgatcgag ccgccgatgt ccagtcgacg
     7861 gatcgcatag gccgcctctt ccgcggccgc gagggccagc tcgaagggcg gaaggaagcc
     7921 gtcgacgtcg aacagggtca tggcgggtgt caggctcatg cgcagctcgc cgccggcgaa
     7981 gtcgatcggg gtgccgagcg cctcgtcgat cagctcggac cagccggcct catagaaaag
     8041 gtcggggccg agccgcggaa gcgggcgcgg cgagccgatc cgctcgccga ggctcggccc
     8101 ttccttctcc tccgcatcgg tgatccggcc cttggcgaac ttgggatggc cgggttcggc
     8161 gatggcttcg cggacgatct cgacccgcac cttc
//
LOCUS       DASVQZ010000246         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_725766, whole genome shotgun sequence.
ACCESSION   DASVQZ010000246 DASVQZ010000000
VERSION     DASVQZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725766"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1027)
                     /locus_tag="VF547_06680"
     CDS             complement(92..1027)
                     /locus_tag="VF547_06680"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX8262540.1"
                     /translation="MSALDTRLLACDAASIAEAARLIRAGLPVAVPTETVYGLAADAT
                     DAEAVAAIYAAKGRPSFNPLIVHVASLDQAELYGDFSDRARDLAAACWPGALTLVVPA
                     RPDNGLAAAVTAGLGTVALRCPAHPAMQALLAAAGRPLAAPSANASGTISPTRAEHVL
                     ASLGGRIPLILDGGPTERGLESTIVAVEGERVRLLRPGPIEVAGREAPLPSAPDRVEA
                     PGQLRSHYAPAKPLRLDCDWAADDEWLIGFGAIAGDDNLSPAGDLAEAAARLFDALHR
                     ADREDRPRIAVAPIPAEGIGVAVNDRLRRAAAPRG"
     gene            1210..1602
                     /locus_tag="VF547_06685"
     CDS             1210..1602
                     /locus_tag="VF547_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262541.1"
                     /translation="MGVTAIKEVVNNSPHTVHLYNTENDKKFMNGGDIAPFSTQAVDM
                     WIPWVPVDWHLRQHHIKIAIGDDYDHRKRFAIYQYADVDGDFVRMYRSGPDGTAGLTR
                     NRIGGEARVDGDRRMIIAADGEISLERI"
BASE COUNT          288 a          542 c          630 g          279 t
ORIGIN      
        1 ggaggtcaag gtccaggcgg cgacggcgcc gaccacgcct cagtagcagc tcgccgtccg
       61 gggaaagcgc gggcttcgtc caccctttcc ctcatccccg cggcgccgcg gcgcggcgga
      121 gccggtcgtt gacggcgacg ccgatgcctt ccgccgggat cggcgcgacc gcgatccgcg
      181 ggcggtcctc gcggtcggcg cggtggaggg cgtcgaacag ccgggcggcg gcctcggcga
      241 gatcgccggc cggcgagaga ttgtcgtcgc cggcgatcgc gccgaagccg atcagccatt
      301 cgtcgtccgc cgcccaatcg cagtcgagcc ggagcggctt ggccggcgca taatggctgc
      361 gcagctggcc cggagcctcg accctgtccg gcgcggacgg gagcggcgcc tccctcccgg
      421 ccacctcgat cggccccgga cggagcagcc gcacgcgctc gccctcgacg gcgacgatgg
      481 tcgattccag gccccgctcg gtcggcccgc cgtcgaggat caagggaatc cggccgccga
      541 ggctggctag gacatgttcg gcgcgggtcg ggctgatcgt tccgctggcg ttggccgagg
      601 gggcggcgag ggggcgcccg gccgcggcca gcagagcctg catcgcggga tgggccgggc
      661 agcgcagggc gacggtgccg agcccggcgg tgacggcggc ggcgagaccg ttgtccggac
      721 gcgccggcac caccagggtg agggcgcccg gccagcaggc ggccgccagg tcccgcgccc
      781 tgtccgagaa gtcgccgtag agctcggcct gatcgaggct cgcgacatgg acgatcagcg
      841 ggttgaacga ggggcggcct ttcgccgcgt agatggcggc gaccgcctcg gcgtcggtcg
      901 cgtcggcggc gaggccgtag accgtctcgg tcggaaccgc gaccggaagc ccggcgcgga
      961 tcagccgcgc cgcctccgcg atcgaggcgg catcgcaggc aagcaggcgg gtatccaacg
     1021 cgctcatttc gcgcgggcta gcgcgtccgc agggggcctg caacgacctg gcctcccccg
     1081 agcgacggcg tgggcggatc tttgagttta tcgacggaag gaatgtgccg tcgaagaagt
     1141 tcttatcttt gtccgacggc cgatgtatag ttgcgtcgag gtggtgcctc tctgatgagg
     1201 agtaactcca tgggcgtcac ggcgatcaag gaggtggtca ataacagccc gcacaccgtt
     1261 catctgtaca atacagaaaa cgacaaaaag tttatgaatg gcggtgatat cgcgcccttc
     1321 agcacccagg ctgtggacat gtggatccct tgggttcccg tcgattggca tcttcgtcaa
     1381 catcacataa agattgccat cggcgacgat tatgatcacc ggaaaaggtt cgcgatttat
     1441 caatatgccg acgtcgatgg cgatttcgtt cgaatgtacc gatcggggcc ggacggcact
     1501 gccggactta ctcgcaaccg tataggcggg gaagcgaggg tggacggaga tcgtcgaatg
     1561 atcatcgcgg ccgatggcga gatttccctg gagaggatct agagcatgat cgcgcttcgt
     1621 tgaatcgaaa ggggattccc aagacggctg gcttctgatt caacatgctg gctgggagga
     1681 ggccagcatg gatgaggcct tattcgactg atcttcggga gcgcgtggtt gcggcgatg
//
LOCUS       DASVQZ010000247         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_725802, whole genome shotgun sequence.
ACCESSION   DASVQZ010000247 DASVQZ010000000
VERSION     DASVQZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725802"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..480)
                     /locus_tag="VF547_06690"
     CDS             complement(217..480)
                     /locus_tag="VF547_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8262542.1"
                     /translation="MANRRNGTLYTGVTSDLPARGWQHKNGKTGFAARYGCKMLVWYE
                     LHDDMTHAITREKRIKAGSRGKKLALIEALNPEWKDLYESIVW"
     gene            complement(652..1494)
                     /locus_tag="VF547_06695"
     CDS             complement(652..1494)
                     /locus_tag="VF547_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8262543.1"
                     /translation="MRFEGTESYVATDDLKVAVNAAVTLRRPLLVKGEPGTGKTILAH
                     EIAAALGAPLIEWHVKSTTKAHQGLYEYDAVARLRDGQLGDPRVHDIANYIRRGKLWE
                     AFTSPTLPVLLIDEIDKADIEFPNDLLQELDRMEFHVYETRETVAARERPIVVITSNN
                     EKELPDAFLRRCFFHYIKFPDRETMQAIVDVHFPGIQKMLVSKAMDIFYQVREVPGLK
                     KKPSTSELIDWLKLLLHEDMPLEVLQSRDPSKAIPPLHGALLKNEQDVMLFERLAFMA
                     RRQP"
     gene            complement(1657..>2060)
                     /locus_tag="VF547_06700"
     CDS             complement(1657..>2060)
                     /locus_tag="VF547_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262544.1"
                     /translation="GERAYLARLAVAAGPVPSTPGSAGTDAAVLGQRHAMETLASSER
                     RGCALGAAMALVLDWSAIRVCLDSAAVRFGVDSPVYAAADRETLVALADSYAGSAPVQ
                     RALLFGAEQILIQHHGLWDLLEARREARAAG"
BASE COUNT          326 a          639 c          728 g          367 t
ORIGIN      
        1 atggacctcg gccagggccg ctacctcccg gtcaagaacg tcttcgtccc gctctggatc
       61 ggcggccgcc gctggggcaa tttcgagctc gcctaccgcg acgaagcgat gcgcatctaa
      121 cccgtcattg cgaggagcga agcgacgcgg caatccagtg ccggcgtcga ttaccagcgc
      181 tgactggatt gcttcgcatt cgctcgcaat gacggcttac cagactatgc tctcgtagag
      241 gtctttccat tctggattga gcgcttcgat cagggccagc ttcttgcctc gtgatccggc
      301 cttgatccgc ttctcgcgcg tgatggcgtg cgtcatgtcg tcgtgcagtt cgtaccagac
      361 gagcatcttg caaccgtatc gggcggcgaa gccggtcttg ccattcttat gctgccaacc
      421 gcgcgccggc aggtccgaag tgaccccagt gtagagcgtg ccgttgcgcc ggttggccat
      481 tatgtagacg gcgggttgct tcacgaaaac ccgaatgcat cggttagaag cacaacgcaa
      541 tgagatgaat catcggcccg tcattgcgag gagcgcagcg acgcggcaat ccgctctcgg
      601 tgcctggctc gcacgccgcg ctggattgct tcgctaggct cgcaatgacg gttagggttg
      661 ccgccttgcc atgaacgcga ggcgctcgaa gagcatcacg tcctgctcgt tcttgaggag
      721 ggcgccgtgg aggggcggga tcgccttgga ggggtcgcgc gactggagga cctcgagcgg
      781 catgtcctcg tggaggagca gcttcagcca gtcgatcagc tcgctggtcg agggcttctt
      841 cttgaggccg gggacctcgc ggacctggta gaagatgtcc atcgccttcg agaccagcat
      901 cttctggatg cccgggaaat ggacgtcgac gatcgcctgc atcgtctcgc ggtcggggaa
      961 cttgatgtag tggaagaagc agcggcgcaa gaaggcgtcg ggaagctcct tctcgttgtt
     1021 cgaggtgatg acgacgatcg gccgctccct cgccgcgacc gtctcgcggg tctcgtagac
     1081 gtggaactcc atccggtcca attcctggag gaggtcgttg gggaactcga tgtcggcctt
     1141 gtcgatctcg tcgatcagca ggacggggag ggtcggcgag gtgaaggcct cccacaattt
     1201 gccgcggcgg atgtagttgg cgatgtcgtg gaccctggga tcgccgagct ggccgtcgcg
     1261 caggcgcgcc accgcgtcat attcgtagag gccctggtgg gccttggtgg tcgacttgac
     1321 gtgccattcg atcagcgggg cgccgagggc ggcggcgatc tcgtgggcga ggatcgtctt
     1381 gccggttccc ggctcgccct tgacgagcag cggtcgcctc agcgtgacgg cggcgttgac
     1441 cgcgaccttg aggtcgtcgg tggcgacata gctctcggtg ccttcgaagc gcatcgcggc
     1501 gggatagggg agggggccgt tggagacaat gctcggcgtg aagaaatgcc gttcgggctg
     1561 agcctgccga aagcccgttc ctcctgtcgc cggcagaagg catgggcttc gacaagccca
     1621 gcccgaacgg ctcctgggag ccgccgcttc tccgcttcag cccgccgccc gggcctcgcg
     1681 gcgggcctcg agcaggtccc acaggccgtg atgctggatg aggatctgct cggccccgaa
     1741 cagcagcgcg cgctgcaccg gcgccgagcc ggcataggaa tcggcgagcg cgacgagggt
     1801 ctcgcggtcg gcggcggcat agaccggcga gtcgaccccg aaccgcacgg cggcggagtc
     1861 gaggcagacc cggatcgcgc tccagtcgag caccagcgcc atggcggcgc cgagcgcgca
     1921 gccgcgccgc tccgacgagg cgagggtctc catcgcgtgg cgctggccga gcacggccgc
     1981 gtcggttccg gcggagcccg gggtcgaggg caccggaccg gcggcgacgg cgagccgggc
     2041 gagataggcg cgctcgccgg
//
LOCUS       DASVQZ010000248         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_246179, whole genome shotgun sequence.
ACCESSION   DASVQZ010000248 DASVQZ010000000
VERSION     DASVQZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246179"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF547_06705"
     CDS             <1..282
                     /locus_tag="VF547_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262545.1"
                     /translation="FETIYPGWYPGRATHVHCKAWLPEGRAEIASQLYFPDALSDSVY
                     AASPYAGREGRRLRNAQDGLFAAAGEAAPVAETRRSAAGLDAALVLALR"
     gene            551..1162
                     /locus_tag="VF547_06710"
     CDS             551..1162
                     /locus_tag="VF547_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8262546.1"
                     /translation="MKASFLIAAAAAALAIQPAGAALAEAEPASPDRAAAETSGFRAE
                     ALAGFDNDGFDHGMLYGGRLGYDLRVAPRLLLGIDAEYSDVTTDEEFAFPGLPSLTAE
                     DGPEYYVGGRATFVLSKRFRLFGAGGYTRGKQGFFFQSDPNPAPLGTVASGRRSLDGF
                     RLSAGGQVLLGRHAFLGAEYRYSNYDDFGLDREQLVGSIGFRF"
     gene            complement(1231..1815)
                     /locus_tag="VF547_06715"
     CDS             complement(1231..1815)
                     /locus_tag="VF547_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8262547.1"
                     /translation="MSIVRERLSPGESRAAALEAARALLIESGPQAVTLKAVAARIGK
                     THGNLLHHFGSAAGLQGELARLIGERVTAEIARVVERERQGEADPREIVDRTFDAFDR
                     EGAGALAAWMILSGNRDALDPILDAIHRLVDKLGAGHEDRPVHDTTLWLVLAALGDSL
                     LGEPMARALGLPREQAREIATRHLVHLASERGAP"
     gene            1860..2699
                     /locus_tag="VF547_06720"
     CDS             1860..2699
                     /locus_tag="VF547_06720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX8262548.1"
                     /translation="MGREVTPSDLTITPRDRRFGRGSKMERWWLGGNPYGSALYDALS
                     VTFPKGEAFFVESVRAFREDAPPKLAEEIRAFTTQEVMHSREHVAFNRRAVEAGYDIA
                     PLEARVDKRLEETKGRPPVVNLAATMALEHFTAVIANQLLGNPRHLKDADPQSAALWR
                     WHSMEEIEHKGVAYDTWLHATRNWPRRKRWTVKALVMLKVTRNFFVERTQGALILLAQ
                     DGIRGPRAWAGLFWYAMVSPGMFRKILGAWLKFFLPGFHPWKHDDRKLIAEGERSLEA
                     LPA"
     gene            complement(2832..>3327)
                     /locus_tag="VF547_06725"
     CDS             complement(2832..>3327)
                     /locus_tag="VF547_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8262549.1"
                     /translation="ALFEAIADERIVRNLATAPWPYSLADAEAFLATERKPSEPSLLI
                     FRCTADAPELIGGIGFGLRPSGEREFGYWLARRHWGRGYATEAGRALLATARLGLRLK
                     RLDAGHFLDNPASGRVLEKLGFRPTGVIAPRYSAGRGGMAPCRLFELELDTESEIAPA
                     AMAA"
BASE COUNT          494 a         1194 c         1164 g          475 t
ORIGIN      
        1 ttcgagacga tctatccggg ctggtatccc ggccgagcga cccacgtcca ttgcaaggcc
       61 tggctacccg agggacgggc ggagatcgcc tcgcagctct atttccccga cgccttgtcc
      121 gactccgtct acgccgcatc gccttatgcg ggccgcgagg ggcggcgcct gcgcaacgcc
      181 caggacggcc tgttcgcggc cgccggcgaa gcggcgccgg tcgccgagac ccggcggagc
      241 gccgccgggc tcgacgccgc tttggtgctg gccctgcgct gagccgcgga tttccgggcc
      301 gtcctccgcc gcatcccggc cgcagaaggg ccgcaatcgc cggacagttt cgcgctcgga
      361 acggaccgga cggcagaagc gctcgagacg cgcgtcgcca agcagcgggg gcgctgccgg
      421 ccatgggtcc gtttcaacgt cccggcgcgg cgcttaacag gcgccgccgc cgccaggcag
      481 ccgccagacg ctgccggaag ggccgtccgt cgaggcgcgc ttcccacggc aacggggcgg
      541 gagagagacg atgaaagcaa gcttcctgat cgcagccgcg gctgccgccc ttgcgatcca
      601 gccggccggc gcggccctgg ccgaggcgga gccggcgagc ccggaccgcg ccgcggcgga
      661 gacttccggc ttccgggccg aggctctggc cggattcgac aatgacgggt tcgaccacgg
      721 catgctctac ggcggccggc tcggctacga cctgagggtt gccccgcggc ttctgctcgg
      781 catcgacgcc gaatatagcg acgtcaccac cgacgaggag ttcgcctttc caggccttcc
      841 cagcctgacc gcggaggacg ggccggaata ttatgtcggc ggccgggcga ccttcgtcct
      901 gtcgaagcga ttccgcctct tcggcgctgg cggctatacg cgcggcaagc agggcttctt
      961 cttccagagc gaccccaatc cggccccgct cggcaccgtc gcgtctggcc gccgctcgct
     1021 cgacggcttc cggctgagcg cgggcggcca ggtgctgctc ggccgccatg ccttcctcgg
     1081 cgccgaatat cgctattcca actatgacga tttcggactc gaccgcgagc agctggtcgg
     1141 aagcatcggc ttccgcttct aggcggcaac cgcgcgaacg accccggccc ctctcctgca
     1201 gtcagcggga gaggggaagg gcgaagcccg tcacggcgcg ccccgctcgc tggcgagatg
     1261 gacgaggtga cgggtggcga tctcgcgcgc ctgctcgcgg ggcaggccga gcgccctcgc
     1321 catcggctcg ccgagcagcg aatccccgag cgcggccagc acgagccaga gcgtcgtgtc
     1381 gtggaccggt cggtcctcgt ggccggcgcc gagcttgtcg accaggcggt ggatggcgtc
     1441 gagaatgggg tcgagagcgt cgcgattgcc gctgaggatc atccaggcgg cgagcgcccc
     1501 ggcgccctcc cggtcgaagg cgtcgaacgt ccggtccacg atctcgcgcg gatcggcctc
     1561 gccctggcgc tcgcgctcga cgacccgcgc gatctcggcg gtgacccgct cgccgatcag
     1621 ccgcgccagc tcgccctgca gcccggcggc tgagccgaaa tggtggagga ggttgccgtg
     1681 ggtcttgccg atccgggcgg ccaccgcctt caaggtcacc gcctgcggcc ccgattcgat
     1741 cagcagcgcg cgcgccgcct ccagcgccgc ggcccggctc tccccggggg acaggcgttc
     1801 acgaactatt gacatctatg taagtatggg tcattcgtca tggacgaagg agatacagca
     1861 tggggcgcga agtcacaccc tccgatctga cgatcacgcc gcgcgaccgg cgcttcggcc
     1921 gcggcagcaa gatggagcgc tggtggctcg gcggcaatcc ctatggcagc gccctctacg
     1981 acgcgctgtc ggtcaccttc ccgaagggcg aggccttctt cgtcgagagc gtccgcgcct
     2041 tccgggagga cgccccgccc aagctcgcgg aggagatccg cgccttcacc acccaggagg
     2101 tgatgcacag ccgcgagcat gtcgcgttca accggcgcgc ggtcgaggcc ggctacgaca
     2161 tcgcgccgct cgaggcgcgg gtcgacaagc ggctcgagga gaccaagggc cgcccgccgg
     2221 tggtcaacct cgcggcgacc atggcgctcg agcatttcac cgcggtcatc gccaaccagc
     2281 tgctcggcaa tccgcgccac ctcaaggacg ccgatcccca gtccgcggct ctgtggcggt
     2341 ggcactcgat ggaggagatc gagcacaagg gcgtcgccta cgacacctgg ctccacgcca
     2401 cccgcaactg gccccgccgc aagcgctgga cggtgaaggc gctggtgatg ctcaaggtga
     2461 cccgcaactt cttcgtcgag cgcacccagg gcgccctgat cctgctcgcg caggacggga
     2521 tcagggggcc gcgcgcctgg gccgggctgt tctggtacgc gatggtcagc cccggcatgt
     2581 tccgcaagat cctcggtgcc tggctgaagt tcttcctgcc cggcttccac ccctggaagc
     2641 atgacgaccg caagctgatc gccgagggcg agcggagcct ggaagccctg ccggcctgaa
     2701 atcctcccct gcatttgcag gggaggggga ccatgcgcag catggtggag gggccttgct
     2761 gagtccgaag aacccctcca ccacgcttcg cgcggtcccc ctccccgaga aatctcgggg
     2821 aggaacttca ttcacgccgc catcgccgcc ggggcgattt ccgactccgt gtcgagctcc
     2881 agctcgaaca gccggcaagg cgccatcccg ccgcggccgg cgctgtagcg cggggcgatc
     2941 acgcccgtgg gcctgaagcc gagcttctcg agcacccgcc cggaggccgg attgtcgagg
     3001 aagtggccgg cgtcgagccg tttcaggcga aggccgaggc gcgcggtggc gagcagggcc
     3061 cggccggcct cggtggcgta gccgcggccc cagtgccggc gcgcgagcca atagccgaac
     3121 tccctctcgc cgctcgggcg aagcccgaag ccgatgccgc cgatcagctc gggcgcgtcg
     3181 gcggtgcagc ggaagatgag cagcgacggc tcggacggct tgcgctcggt ggcgaggaag
     3241 gcctcggcgt ccgccaggct atagggccag ggcgccgtcg cgaggttgcg gacgatgcgc
     3301 tcgtcggcga tcgcctcgaa cagggca
//
LOCUS       DASVQZ010000249        10930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_918000, whole genome shotgun sequence.
ACCESSION   DASVQZ010000249 DASVQZ010000000
VERSION     DASVQZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918000"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1755
                     /locus_tag="VF547_06730"
     CDS             292..1755
                     /locus_tag="VF547_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8262550.1"
                     /translation="MRPDPTRCLLLIDDEPAQRRLVTAIGARAGWWVRGAPDIDAAAR
                     ILAEVDGPPVDAILLDHWLPGPDGSHVIEQLRAMRPDLPLLVMTAQTSVAVAVEAMRA
                     GAHDFLVKPIAPDSLLAALNAATDRRQRGGGELRPLSEKISVRPLAFEEIVGSAPPFR
                     SALAVAAKAARARVSVLIEGESGSGKEVIAQAIHTASPRGRKPMVTVNCGAIPENLVE
                     SVLFGHEKGAFTGAFDRHVGRFEDADGSTLFLDEVGELPLDTQVKLLRALETGEIQRI
                     GGRVIHTVDVRIIAATNRRLAEEVARGRFREDLYYRLNVVHVPIPPLRDRASDIPPLA
                     RHLLARIAEQPGMRHLSITDDGLEVLMRYGWPGNVRQLQNALFRAAVLCDGNALTAAD
                     FPQIAQESTLSRRASDFHRPALNGASQQAALSNGPGITLYDPDGNLRPLEAIEADVIR
                     LAIGHYRGRMTEVARRLGIGRSTLYRKLGELGIGDAA"
     gene            1882..2631
                     /locus_tag="VF547_06735"
     CDS             1882..2631
                     /locus_tag="VF547_06735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8262551.1"
                     /translation="MHDFQNAIVLITGAASGIGAATATRLAEGGARKLILMDRDEDRL
                     RDFAFSLPCERQIRIGDVADEGLWEREDLTGLTHAVINAGIGEGGPIADTAFADWRRV
                     MSINLDGAFLTLQAAMKAMRDTGGAIVLTASAAGLKAEAGIAAYGASKAAVIHLARIA
                     AKEGAPLGIRVNSIAPGGVETAIWRGVPAFAEMVERQGEAAAFKAMAAGTPLARFGKP
                     DEIAAQIAFLLSDDAAFMTGACLVSDGGYSL"
     gene            2891..3391
                     /locus_tag="VF547_06740"
     CDS             2891..3391
                     /locus_tag="VF547_06740"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX8262552.1"
                     /translation="MNAGMYDEGGRPIGLYIENGVQRRPLNLRAGPGNFHLLPNGVFA
                     VDEGGRVSVTPSKLFAKASRRARWATQSGPMLLIGGKLHPKFDADGPSRLVRNGVGVA
                     GPRTAWFAISEEGVSFGRFARLFRDLGCTDALYLDGTVSSLWDPGAGRKDAYSSLGPM
                     IAVFGP"
     gene            3426..4214
                     /locus_tag="VF547_06745"
     CDS             3426..4214
                     /locus_tag="VF547_06745"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEX8262553.1"
                     /translation="MRLPASPALLLLALAGAPASAERLDYRIVEDDGLAAPARLPYDK
                     VEGDTEPAGFKLQWALLDRFEYAGQSGRDGYAWDFSALVGGDRHRVWLGTNGEGAAWG
                     APDYVELNALYSYRFARAWDANAGLRYDAQSGPDRAYAALGAQYDDGEALWLGAWAYL
                     STKGELSARLAGYYNQKLVGALILQPSAELDYYGEDMPELGLGRGFGYAEAGLRLRYE
                     IKEAFAPYVGLSWSRDLGRTARMTRAEGEDPETKSLVMGVRSAF"
     gene            complement(4198..4497)
                     /locus_tag="VF547_06750"
     CDS             complement(4198..4497)
                     /locus_tag="VF547_06750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8262554.1"
                     /translation="MDGEVRNNEGEQRYELEVDGEIAVAEYRLRPGRITFTHTKVPEA
                     LEGRGIGTRLVKAVLDDARAQGLRVVPTCPFVKHYIDTHPEYGDLLDDPAVKMRT"
     gene            complement(4610..5644)
                     /locus_tag="VF547_06755"
     CDS             complement(4610..5644)
                     /locus_tag="VF547_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8262555.1"
                     /translation="MRFGYWMPVFGGWLRNVPDEGMEASWDYVRRLTLRSEETGWDLS
                     LIAELNLNDIKGVEEPALDAWSTAAALAAVTERLELMVAVRPNFHQPALFAKQAANID
                     NISGGRLSLNVVSSWWADEATQYGLQFDRHDDRYARTREWLEVVDGLWTEKRFSYEGE
                     RYRLKDAICEPKPVRKPRPTIYAGGESEAAKDMIARQCDAYVMHGDPVEAIRPKIAGM
                     AARREQVGAPPMQYGMAAYAIVRDSEAEARRELERITAMPVQPPSGFDNFDQWLSGTE
                     LERELKLQEYSVSNRGLRPGFVGTPEQLRERIEEYEEAGLDLLLLQMSPQAEEMDRFA
                     EQVIGPMKAS"
     gene            complement(5646..6566)
                     /locus_tag="VF547_06760"
     CDS             complement(5646..6566)
                     /locus_tag="VF547_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-glutamate ligase"
                     /protein_id="HEX8262556.1"
                     /translation="MKSDLAILYEHPTWFEPLFAALERRAVRFEAIRLSDHRFDPAST
                     EIPAPVVLSRVAMSSFLREREHGIFYAEALLAHWAANGARVLNGAGVIAVDSSKARQL
                     SLISRLGYCVPETRVVHRREDLLAAAGTMRWPLLVKANIGGSGAGIVRYGGEAELAAS
                     VAEGTVPESVDSVLLVQDHVPARGGAITRVETLGGKFLYAIEVESRGDSFDLCPADAC
                     LAQPGRAAVRMEAATPPAQLIEAAERIAQAAGLAVGGVEYLIDERDGRARFYDINALS
                     NFVAKPLDVLGWDPHDRLVDFLEAAIRETR"
     gene            complement(6700..8670)
                     /locus_tag="VF547_06765"
     CDS             complement(6700..8670)
                     /locus_tag="VF547_06765"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX8262557.1"
                     /translation="MVDFAAERERMVRRQIAGRGIKARRLLDAFRTVPREEFVPEGVR
                     PFAYEDGPLPIEAEQTISQPYIVAAMIEAAEVGPGERVLEIGAGSGYAAAVMSRIAGE
                     VVAVERHGELVQLARSRMARLGYDNVRIVQGDGTSGLPDEAPFEAILCAASGSHVPPV
                     LLDQLSVGGTLVMPVGEPNSVQKLVKVTRRAAEEFQEEDLGPVRFVPLIGAHGWADPK
                     GGEAMTVPERIAAAAEPLPEIDDPAFAEAFDRFADARVIMLGEASHGTSEFYRARAAI
                     TRRLIERHGFSIVAVEADWPDAASMDRYVRHRPKREGEEAAFQRFPTWMWRNTDVEAF
                     AGWLRRHNSGRAYEAMAGFYGLDLYNLAGSIRAVIDFLDEADPEAARVARKRYGCLRP
                     WAHDPAEYGRIAVTQGFSRCEAGVVQMLKELMTRRMDCLGPECDEWLDAAANARLIKN
                     AEQYYRVMYYGSAESWNLRDTHMFETLCQLLEAKGPQSKAVVWAHNSHIGNAAHTEMG
                     QVREELNIGQLVKERFGREARLIGFGTHRGTVAAASDWDEPMEVKEVRPSLPDSYERL
                     CHDSRVPRFLLDLREGVNNEAVEALLEPRLERFIGVIYRPETERWSHYAEAVLPNQFD
                     AWVWFDETEAVTPLPGGEEPATGEDETYPFGL"
     gene            complement(8722..9063)
                     /locus_tag="VF547_06770"
     CDS             complement(8722..9063)
                     /locus_tag="VF547_06770"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX8262558.1"
                     /translation="MKKHWKAIAVVLVVVAGLAWFAIDAMRERAAMTAAATATVTRVA
                     FDPDEESSSLDETDIELIFVAKGQQVTASTSLPGDRVKDYPVGRDVAICYDPKNPATS
                     RIDSDGGGCGG"
     gene            9159..9755
                     /locus_tag="VF547_06775"
     CDS             9159..9755
                     /locus_tag="VF547_06775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8262559.1"
                     /translation="MHQRIIGLYDENAAAWDALRGPDARLERKWLERFADLLPAGGRI
                     LDIGCGTGRPIAGWLTDRGLRVTGVDSSPALIAIARKRFPEARWHVADMRALALPERF
                     DGLVAWHSLFHLSPEDQRPVFARMAAHAAPGAILMFTSGCSEGIRVGQWQGEPLYHAS
                     LDPAEYRRLLEQNGFEPLDCRLRDPQCGEASVWIARRL"
     gene            complement(9816..>10930)
                     /locus_tag="VF547_06780"
     CDS             complement(9816..>10930)
                     /locus_tag="VF547_06780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX8262560.1"
                     /translation="QALNRLVEYLLRQGAAVRIYSPTVAEPAFPPNGEVVHIPSLSIP
                     RRKEYKFPVRLPARARRDIRAFRPNIFHVASPDMLGHRAVTLARQLDVPVVASVHTRF
                     ETYFRYYGLAFLEPLVQAILRRFYRRCDAIFAPSDSMAQLLREQRMSYNVGIWTRGID
                     RGIFNPDRRDLAWRRSLGIGDDEPVVGFVGRMVMEKGLDVFSETIDCLERRQVRHKVL
                     AVGEGPAREWLQHRLPDAVFAGFQQGPELGRAVASMDILFNPSVTETFGNVTLEAMAA
                     GLPVVAAIATGSQSLVEDGVTGRLVRAGAIDGFAAALEAYCRDPDKRRAAGRAGCEAS
                     RRYGWDEVNQALVDGYLRVIRQRQGGGRRPSASPVP"
BASE COUNT         1589 a         4046 c         3634 g         1661 t
ORIGIN      
        1 agccgggccg agtcaaaccg ggattggtcc ggaacggccg cggctccccc gagccgcggg
       61 cccccgccgt ccccccgcgc ggcccggcgc gggcccgccg gaccccggtg cgcgccccgc
      121 gcttccacgc cagggtgaga acagatatag aacatgctcc agccgccggc aagcccgatt
      181 gcttccgatc cggagcaaac tcgttcgcgg ctccgtcccg ggcgtcccgt tttaagccga
      241 actttacccg gcccgcctaa agccggtcgc gacgacgccg aaggagggac gatgcggccg
      301 gatccgacac gctgcctgct gctgatcgac gacgagcccg cccagcgccg gctggtcacc
      361 gccatcggcg cccgagccgg ctggtgggtc cgcggcgcgc ccgacatcga cgccgcggcg
      421 cggatcctgg ccgaggtcga cggaccgccg gtcgacgcca tcctgctcga ccattggctg
      481 cccggccccg acggcagcca cgtcatcgag cagctgcgcg cgatgcgccc cgaccttccc
      541 ctgctggtga tgaccgccca gacctcggtc gcggtcgcgg tcgaggcgat gcgcgccggc
      601 gcccacgact tcctggtcaa gccgatcgcg cccgacagcc tgctcgccgc cctcaatgcc
      661 gccaccgacc ggcgccagcg cggcggcggc gagcttcggc cgctcagcga gaagatctcg
      721 gtccggccgc tcgccttcga ggagatcgtc ggctcggcgc cccccttccg ctccgccctc
      781 gccgtcgccg ccaaggcggc ccgagcccgg gtctcggtgc tgatcgaggg cgagagcggc
      841 tcgggcaagg aagtgatcgc ccaggcgatc cataccgctt ccccgcgcgg ccgcaagccg
      901 atggtcaccg tcaattgcgg cgccatcccc gagaacctcg tcgaatcggt gctgttcggc
      961 catgagaagg gcgccttcac cggcgccttc gaccgccatg tcggccgctt cgaggatgcc
     1021 gacggctcga ccctgttcct cgacgaggtc ggcgagctgc cgctcgacac ccaggtgaag
     1081 ctgcttcggg cgctcgagac gggcgagatc cagcgcatcg gcggccgggt catccacacc
     1141 gtcgacgtcc ggatcatcgc cgcgaccaac cggcgcctcg ccgaggaggt cgcccgcggc
     1201 cgcttccgcg aggatctcta ctaccgcctc aacgtcgtcc acgtgccgat cccgcccttg
     1261 cgcgaccgcg cctcggacat cccgccgctc gcccgccacc tcctcgcccg gatcgccgag
     1321 cagccgggca tgcgccattt gtcgatcacc gacgacgggc tcgaggtgct gatgcgctac
     1381 ggctggccgg gcaacgtccg ccagctccag aacgccttgt tccgggccgc ggtgctgtgc
     1441 gacggcaatg cgctcaccgc cgccgacttc ccgcagatcg cccaggaatc gacgctctcg
     1501 cgccgagcca gcgacttcca ccggcccgcc ctgaacggcg cctcgcagca ggcggccctg
     1561 tccaacggcc ccggcatcac cctctacgac cccgacggca accttcggcc cctggaggcg
     1621 atcgaggccg acgtcatccg actcgccatc ggccattatc gcggccggat gaccgaggtg
     1681 gcgcggcgcc tggggatcgg ccgctcgacc ctctaccgca agctcggcga gctcggcatc
     1741 ggcgacgccg cctgacgacg gtcgggttcg acacaatcgc tcatcctgag tagggactga
     1801 gcctggcgac ggcccgtagc gaaggacgtc cttcgatacg ccgcttcggc ttcgctcagc
     1861 ggctactcag gatgagcgga tatgcatgac ttccagaacg ccatcgtcct catcaccggc
     1921 gccgcctccg gaatcggcgc ggcgaccgcc acccgcctcg ccgagggcgg cgcccgcaag
     1981 ctgatcctga tggaccgcga cgaggaccgc ctgcgcgact tcgccttctc cctgccctgc
     2041 gagcgccaga tccgcatcgg cgacgtcgcc gacgaggggc tttgggaacg cgaggacctg
     2101 accggcctca cccacgccgt gatcaacgcc ggcatcggcg agggcgggcc gatcgccgac
     2161 accgccttcg ccgactggcg ccgcgtcatg tcgatcaacc tcgacggcgc cttcctcacc
     2221 ctccaggccg cgatgaaggc gatgcgcgac accggcggcg ccatcgtgct gaccgcctcg
     2281 gccgccggcc tcaaggcaga ggcggggatc gccgcctacg gcgcctccaa ggccgccgtc
     2341 atccacctcg cccggatcgc cgcgaaggag ggcgcgccgc tcggcattag ggtcaactcg
     2401 atcgctccgg gcggagtcga gacggcgatc tggcgcggcg ttccggcctt cgccgagatg
     2461 gtggagcgcc agggcgaggc ggcggcgttc aaggcgatgg ccgcgggcac ccccctcgcc
     2521 cgcttcggca agccggacga gatcgccgcc cagatcgcct tcctcctttc cgacgacgcc
     2581 gccttcatga ccggcgcctg cctggtcagc gacggcggct attcgctcta gccttttcct
     2641 cgcagccgcg cttctcgccg gctgccgggc ggaggagcag cctcgcgctc cgccggagcc
     2701 cgctgccgaa gccgcccggt cgccggcctg cgagccgcgg catttcgagg gaagccgctt
     2761 caccgtctgc cgctacgagg ccggccgcga ccgcctcgcc ctcttcctcg acggggcgcc
     2821 gggcccgctg cgcagcttcg acgcgctgga agcccggctg ggcggcgagg ccaaggcgct
     2881 gcgcttcgcg atgaacgcgg gcatgtacga cgagggcggc cgcccgatcg gcctctacat
     2941 cgagaacggg gtccagcgcc gccccctcaa tctgcgcgcc ggccccggca atttccatct
     3001 gctgcccaac ggcgtcttcg ccgtcgatga aggcgggcgg gtgtcggtca ccccgagcaa
     3061 gcttttcgcc aaggcgtcgc ggcgggcccg ctgggcgact cagtccggac cgatgctgct
     3121 gatcggcggc aagctccatc cgaaattcga tgccgacggg ccgtcacgcc tcgtgcgcaa
     3181 cggcgtcggc gtcgccgggc cccgcaccgc ctggttcgcg atcagcgagg agggcgtctc
     3241 gttcggccgc ttcgcccgcc tgttccgcga cctcggctgc accgacgccc tctatctcga
     3301 cggaaccgtc tccagcctct gggatccggg ggccgggcgc aaggatgctt attcgtccct
     3361 cggtccgatg atcgcggtgt tcggccccta agcggccttt aaccccgggg cgctacgggg
     3421 gtgggatgcg ccttcccgcc tccccggccc tgcttctgct cgccctcgcc ggcgctccgg
     3481 cttcggcgga acgcctcgat taccggatcg tcgaggatga cggcctcgcc gcccccgcgc
     3541 gcctccccta cgacaaggtc gaaggggaca cggagccggc cgggttcaag ctccaatggg
     3601 cgctcctcga ccgtttcgaa tatgccggcc agagcggccg cgacggctat gcctgggatt
     3661 tctcggcgct ggtcgggggc gaccgccacc gggtctggct cggcaccaac ggcgaaggcg
     3721 cggcgtgggg cgcccccgac tatgtcgagc tcaacgccct ctacagctac cgcttcgccc
     3781 gggcgtggga cgccaatgcc ggcctccgct acgacgccca gtcgggcccc gaccgcgcct
     3841 atgcggccct cggcgcccaa tatgacgacg gcgaggcgct ctggctcggc gcctgggcct
     3901 atctgtccac caagggcgag ctgagcgccc gactcgccgg ctattacaac cagaagctcg
     3961 tgggcgcgct gatcctccag cccagcgccg agctggatta ttacggcgag gacatgcccg
     4021 agctcggcct cggccgcggc ttcggctatg ccgaggcggg cctccgcctg cgttacgaga
     4081 tcaaggaggc cttcgccccc tatgtcggcc tcagctggtc gcgcgatctc ggccggacgg
     4141 cgcggatgac ccgcgcggaa ggcgaggatc cggagacgaa gagcctggtg atgggcgtca
     4201 ggtccgcatt ttaaccgcag ggtcgtcgag caggtcccca tattcgggat gcgtgtcgat
     4261 ataatgcttc acgaaggggc aggtcggcac cacccggagc ccctgcgcgc gggcatcgtc
     4321 gaggacggcc ttgaccagcc tcgtgccgat tccccgcccc tccagggcct cgggcacctt
     4381 ggtatgggtg aaggtgatcc gcccgggtcg aagccgatat tcggcgacgg cgatctcgcc
     4441 gtcgacctcg agctcgtagc gctgctcgcc ttcgttgttc ctgacctcac cgtccatccg
     4501 ctctctcctg tcataccgtc attgcgagga gcgcagcgac gaagcaatcc agttgccgag
     4561 tccgcgacga acgccgcagt ggatggcttc gctccgctcg caatgacggt taggacgcct
     4621 tcatcggccc gatcacctgc tcggcgaagc ggtccatctc ctcggcctgg gggctcatct
     4681 ggagcagcag aaggtcgagc ccggcctcct catattcctc gatccgctcg cggagctgct
     4741 cgggcgtgcc cacgaaaccg gggcgaaggc cgcggttcga caccgaatat tcctgcagct
     4801 tcaattcgcg ctccagctcg gttcccgaca gccactggtc gaaattgtcg aagccgctgg
     4861 gcggctgaac gggcatcgcc gtgatccgct ccagctcccg tctcgcctcc gcctcgctgt
     4921 cgcgaacgat cgcataggcg gccattccat attgcatcgg cggcgctccc acctgctcgc
     4981 gccgggccgc catcccggcg atcttgggcc ggatcgcttc gaccgggtcc ccgtgcatga
     5041 cataggcatc gcactgccgg gcgatcatgt ccttggcggc ctcgctctcg cctccggcat
     5101 agatcgtcgg ccgcggcttg cggaccggct tgggctcgca gatcgcgtcc ttcagccggt
     5161 agcgctcgcc ctcatagctg aagcgcttct cggtccagag gccgtcgacc acctcgagcc
     5221 attcgcgggt ccgcgcatag cggtcgtcat ggcggtcgaa ctgaaggccg tactgggtcg
     5281 cctcgtccgc ccaccaggag gagacgacgt tgagcgagag ccgcccgccg ctgatattgt
     5341 cgatattggc cgcctgcttg gcgaacaggg ccggctggtg gaaattgggc cgcacggcca
     5401 ccatcagctc cagccgctcc gtcaccgccg cgagcgccgc ggccgtcgac caggcgtcga
     5461 gcgcgggctc ctccacgccc ttgatgtcgt tgaggttcaa ttcggcgatc aggctgaggt
     5521 cccagccggt ttcctcgctc ctgagggtga ggcgtcggac atagtcccag ctcgcctcca
     5581 tgccctcgtc cgggacgttg cgcagccagc ccccgaagac gggcatccag tagccgaacc
     5641 gcatcctacc ttgtctcccg aatcgcggcc tcgaggaaat cgacgagccg gtcgtgcgga
     5701 tcccagccga gcacgtccag aggcttggcg acgaagttgg acagggcatt gatgtcgtag
     5761 aagcgagccc tgccgtcgcg ctcgtcgatc agatattcga ctccgccgac ggccagccca
     5821 gccgcctggg cgatccgctc ggcggcctcg atcagctggg ccggcggagt cgccgcctcc
     5881 atccggaccg ccgcccggcc cggctgggcg aggcaggcgt cggcggggca cagatcgaag
     5941 ctgtcgcccc ggctctccac ctcgatcgcg tagaggaact tgccgcccag ggtctcgacc
     6001 cgggtgatcg ctccgccgcg cgcgggcaca tgatcctgga ccaggagcac ggagtcgacg
     6061 ctctcgggca ccgttccctc ggcgaccgag gcggcgagct cggcctcacc gccgtagcgg
     6121 acgatgcccg ctcccgagcc gccgatattg gccttgacca gcagcggcca gcgcatcgtc
     6181 ccggcggccg cgagcagatc ctcgcggcga tgcacgaccc gggtctccgg cacgcaatag
     6241 ccgaggcgcg agatgagcga gagctgccgc gccttcgagg aatcgacggc gatgacgccc
     6301 gccccgttca gcacccgggc cccgttcgcg gcccagtggg cgagcagcgc ctcggcgtag
     6361 aagattccgt gctcccgctc cctgaggaag ctggacatcg cgacccggct caaaaccacc
     6421 ggagcgggga tttcggtcga agcggggtcg aaccggtgat cggagaggcg gatcgcctcg
     6481 aagcgcaccg cgcggcgctc cagcgccgca aacagcggct cgaaccaggt cggatgttcg
     6541 tagaggattg cgagatcgga cttcattcga gaggcacttg gggggcggcg tcggcgaatg
     6601 gaagcccccg cccgtcattg cgagcaaagc gaagcaatcc agcggcgcct ccgctcgctg
     6661 gaggcttcgt cgctacgctc ctcgcaatga cgggtgcagc taaagcccga acggataggt
     6721 ctcgtcctcg ccggtggcag gttcctcgcc cccgggcagc ggagtcaccg cctcggtctc
     6781 gtcgaaccac acccaggcgt cgaactggtt ggggagcacc gcctccgcgt agtggctcca
     6841 gcgctcggtc tcgggccggt agatgactcc gatgaagcgc tccagccgcg gctcgagcag
     6901 cgcctcgacg gcctcgttgt tcacgccctc gcgaagatcg agcaggaagc gcggcacgcg
     6961 gctgtcgtgg cacagccgct cgtagctgtc cgggagcgac ggccgaacct ccttcacctc
     7021 catcggctcg tcccagtcgc tcgccgccgc caccgtgccc ctatgggttc cgaagccgat
     7081 cagccgcgcc tcgcggccga accgctcctt gaccagctgg ccgatgttca gctcctcgcg
     7141 cacctgcccc atctcggtat gggcggcgtt gccgatatgg ctgttatgcg cccagacgac
     7201 cgccttcgat tgcggtccct tcgcctcgag cagctggcac agcgtctcga acatgtgggt
     7261 gtcgcgcaga ttccagcttt ccgccgagcc gtaatacatg acccggtaat attgctcggc
     7321 gttcttgatg aggcgcgcat tggccgcggc gtcgagccat tcgtcgcatt cggggccgag
     7381 acagtccatc cgccgggtca tcagctcctt cagcatctgg acgacccccg cttcgcagcg
     7441 gctgaagcct tgggtgaccg cgatccgccc atattcggcg ggatcgtggg cccacggcct
     7501 caggcagccg tagcgcttgc gcgccacccg cgccgcctcg ggatccgcct cgtcgagaaa
     7561 gtcgatgacg gctcggatgg agcctgcgag attatagagg tcgagcccgt agaagccggc
     7621 catcgcctca taggcccggc cggaattgtg ccgcctcagc cacccagcga acgcctcgac
     7681 gtcggtgttg cgccacatcc acgtcgggaa gcgctggaag gccgcttcct cgccttcgcg
     7741 cttcggccgg tggcggacgt agcggtccat gctggcggca tccggccagt ccgcctcgac
     7801 cgccacgatc gagaagccgt gccgctcgat cagccggcgg gtgatcgccg cccgcgcccg
     7861 gtagaattcg gaggtgccgt ggctcgcctc gcccagcatg atcacccgcg cgtcggcgaa
     7921 gcggtcgaac gcctcggcga acgcgggatc gtcgatctcg gggagcggct cggccgccgc
     7981 ggcgatgcgt tcgggcacgg tcatcgcttc acctcctttc gggtcggccc agccgtgggc
     8041 gccgatcagc ggcacgaacc gcaccggccc gagatcctcc tcctggaact cctcggcggc
     8101 gcggcgcgtc accttgacca gcttctggac ggaattgggc tcgccgaccg gcatgacgag
     8161 ggtgccgccg accgacagct ggtcgagaag gaccggcggg acatggctgc cgctcgccgc
     8221 gcacaatatc gcctcgaacg gcgcctcgtc cggcaggccg gaggtgccgt ccccctggac
     8281 gattcgaaca ttgtcgtagc cgaggcgcgc cattcgcgaa cgcgccagct ggacgagctc
     8341 gccatggcgt tcgaccgcga cgacctcgcc ggcgatccgg ctcatcaccg cggccgcata
     8401 gcccgagccg gcgccgatct cgagcacccg ctcgcccggc cccacctccg ccgcctcgat
     8461 catggccgcg acgatatagg gctgcgagat ggtctgctcc gcctcgatcg gcagcggccc
     8521 atcttcatag gcgaacggcc tcacgccttc gggaacgaat tcctccctgg gcaccgtccg
     8581 gaaggcgtcg agcagccgcc gggccttgat gccgcggccg gcgatctgcc tgcgaaccat
     8641 ccgctcgcgc tcggcggcga aatcgaccac tggtccctcc tgtcgcgccg ataaccggcg
     8701 cgggcgggag ggcgttccgg atcagccgcc gcagccgccg ccgtcggagt cgatgcggga
     8761 ggtggccggg ttcttcgggt cgtagcagat ggcgacgtcg cggccgacgg gatagtcctt
     8821 cacccggtcg ccgggcagcg aggtcgaggc cgtcacctgc tgtcccttgg cgacgaagat
     8881 cagctcgatg tcggtctcgt cgaggctgct gctctcctcg tccgggtcga aggcgacgcg
     8941 ggtgaccgtg gcggtcgcgg cggcggtcat cgccgcccgc tcccgcatcg cgtcgatcgc
     9001 gaaccatgcg aggcccgcga ccaccacgag cacgacggcg atggccttcc aatgcttctt
     9061 cactcagccc tccccttcga tccagtgccg ccgcccatat cgtccgcacc gcgcggttca
     9121 agccttgccg ggccggaccg cccgtggaag acagcgccat gcaccagcgc atcatcggcc
     9181 tctacgacga gaatgcggcc gcctgggacg cgctgcgcgg cccggacgcc cggctcgagc
     9241 ggaaatggct cgagcgcttc gcggacctgc tcccggccgg cggcagaatt ctcgacatcg
     9301 gctgcggcac gggccggccg atcgcgggct ggctgaccga tcgcggcttg cgggtgacgg
     9361 gggtcgattc ctcgccggcg ctgatcgcga tcgcccgaaa gcgcttcccc gaggcccgct
     9421 ggcacgtcgc cgacatgcgc gcgctcgccc tgcccgagag gttcgacggc ctcgtcgcct
     9481 ggcacagcct gttccatctc agcccggaag accaacgacc ggtcttcgcc cggatggccg
     9541 cccatgcggc gcccggagcg atcctgatgt tcaccagcgg ctgctccgag gggatcaggg
     9601 tcggccaatg gcagggcgag ccgctctatc atgccagcct cgatccggcc gaatatcgcc
     9661 ggctgctcga gcagaacggc ttcgagccgc tcgactgccg attgcgcgat ccgcagtgcg
     9721 gagaggcgag cgtgtggatc gcccggcgcc tgtgacgccg tgcccgccgg tcgggccgct
     9781 cgccgcccgc cggactcggc aggcggcggc gccgctcacg gcaccgggct cgcggagggc
     9841 cgccggcctc cgccctggcg ctggcggatg acccggagat agccgtccac cagggcctgg
     9901 ttgacctcgt cccagccgta gcggcggctc gcctcgcagc ccgcccggcc ggccgcccgc
     9961 cgtttgtccg ggtcgcggca ataggcttcg agcgcggccg cgaagccgtc gatcgccccg
    10021 gcccggacca ggcggccggt gactccgtcc tcgaccaggc tctggctgcc ggtggcgatc
    10081 gccgccacga cggggagccc cgccgccatc gcctccaggg tgacgttgcc gaaggtctcg
    10141 gtgaccgacg ggttgaacaa tatgtccatc gacgcgaccg cccggccgag ctcgggcccc
    10201 tgctggaagc cggcgaagac cgcatccgga agccggtgct gaagccattc gcgggcgggc
    10261 ccctcgccga cggccaggac cttgtggcgg acctgccgcc gctccaggca gtcgatcgtc
    10321 tcggagaaga cgtccagccc cttctccatc accatccggc cgacgaagcc gaccacgggc
    10381 tcgtcgtcgc cgattccgag cgaccgccgc caggccaggt cgcgccggtc ggggttgaag
    10441 atcccgcggt cgatgccgcg ggtccagatg ccgacattgt agctcatcct ctgctcgcgc
    10501 agcagctgcg ccatcgagtc ggacggcgcg aagatcgcgt cgcagcggcg gtagaagcgg
    10561 cgcaggatcg cctggaccag gggctcgagg aaggcgaggc cgtaatagcg gaaataggtc
    10621 tcgaagcggg tatggaccga agcgaccacc ggcacgtcca gctgccgggc gagcgtgacg
    10681 gcgcggtggc cgagcatgtc ggggctggcg acgtggaaga tgttcggccg gaaggcgcgg
    10741 atgtcgcgcc gcgcccgcgc cggaagcctg accgggaact tgtactcctt gcgacgcggg
    10801 atcgaaaggc tgggtatgtg caccacctcg ccattgggcg ggaaggcagg ctcggccacg
    10861 gtcggcgaat agatccggac cgccgcgccc tgccgaagca gatattcgac cagccggttc
    10921 agcgcctggt
//
LOCUS       DASVQZ010000250         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_436523, whole genome shotgun sequence.
ACCESSION   DASVQZ010000250 DASVQZ010000000
VERSION     DASVQZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436523"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VF547_06785"
     CDS             complement(<1..445)
                     /locus_tag="VF547_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262561.1"
                     /translation="MNPFYKISEMIAELEGTRPKPFRVHMRRLRIILAILYRRMAWYI
                     VYGIALSGAGALLFGIFALENQAVPLLTGLFKIGIAAALGWEHMQFELGPEPLAYPSR
                     SRPQILRRRRVRREEGLDGQLGQGDVDGGAEDGGHAQEGQEAIAGA"
     gene            828..1214
                     /locus_tag="VF547_06790"
     CDS             828..1214
                     /locus_tag="VF547_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8262562.1"
                     /translation="MDREGWAVERIAEGEYAVMEVLWEEAPLTAADVAARVPEDRGWS
                     LATVKTMLSRLLAKGVLTHEEDGRRYLYRPAIRREDYVAEESGRLIDRMFGGRVPALV
                     AQLAARDKLTPADIAEIEKLLKELKS"
     gene            1211..>2104
                     /locus_tag="VF547_06795"
     CDS             1211..>2104
                     /locus_tag="VF547_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX8262563.1"
                     /translation="MIAWLIETLIAVTLLMAMVLVLRGPVARSFGAGWAYALWAVPAL
                     RLVLPPLPQLAPDLYLPPVVLFIPTAAEMAAPLPAEAGPGQWVPFMLAMWAGGAVIFL
                     TLQWLAYRAFLGRIKETSRPARPPLFGGIRTWISDSVDGPLALGVIERRIVLPGDFSR
                     RYNPVERRLALEHELVHHKRGDIWWNLLAMLILAVFWFNPVAWWAFRAFRSDQELACD
                     AAVARTASLDERCDYARALVKSASRPGLIAACALNPAGELKRRLRMMRAHRASPLRSA
                     GGVAALAAFAAAGFALGSAQPA"
BASE COUNT          320 a          714 c          703 g          367 t
ORIGIN      
        1 ccgcgccggc gatcgcctcc tgtccttcct gagcatgacc accatcttcg gcacccccat
       61 cgacatcacc ttgtccgagc tggccatcga ggccttcctc ccggcggacc cggcgacggc
      121 ggagtatctg cgggcgtgag cgggaagggt aagcgagggg ttcgggaccg agctcgaact
      181 gcatgtgctc ccagcccaga gcggcggcga tcccgatctt gaacaggccg gtgaggaggg
      241 gaaccgcctg attctccagc gcgaatatac cgaagagcaa tgcgcccgcg cctgacagcg
      301 ctatgccata gacgatgtac cacgccatcc ggcggtagag gatcgcgagg atgatgcgca
      361 gccggcgcat atgtacccga aacggcttgg gcctcgttcc ctccagttcc gcgatcattt
      421 cggagatctt gtagaacgga ttcatcgggt agccggaaga gacttcgctt catgttggca
      481 acgagcggtg tcgctagtca agtgacctct gctgctatcg gattgtcatt tgtcgtcttg
      541 tcgcggggcg gctcatctca tcgtccccgg cgtccacggg ctgaaaagga aaggggcgga
      601 cggcgggacc agaaagaccg ctcgtccgcc cctgttcgtg tggaagcgtg tggacgccga
      661 tggagataca cgggccgtgc caaagcgtgc cgtgcgcgga tttccgcgct tcgcgatggt
      721 gaacggcaat taacgccttt tgcttattgt aaagcgaacc gacggaagac gttgcgccgc
      781 cgtcttattc atccgccgtt cagtccgaga aggtgattac atgtgtaatc gacagggagg
      841 gatgggccgt ggagagaatc gccgagggcg aatatgcggt gatggaggtg ctctgggagg
      901 aggccccgct gaccgccgcc gacgtcgccg cgagggtgcc ggaggatcgg ggctggagcc
      961 tcgccaccgt caagacgatg ctgtcacggc tgctcgccaa gggcgtcctc acccatgagg
     1021 aggacggccg ccgctatctc taccgaccgg ccatccgccg cgaggattat gtcgccgagg
     1081 aatccggccg gctgatcgac cggatgttcg gcggccgtgt gcccgcgctg gtcgcgcagc
     1141 tcgccgcccg cgacaagctc actcccgccg acatcgccga gatcgagaag ctcctcaagg
     1201 agctcaagtc atgatcgcct ggctgatcga gaccctgatc gccgtcacct tgctgatggc
     1261 gatggtgctg gtccttcgcg gcccggtcgc gcgcagcttc ggcgccggct gggcctacgc
     1321 cttgtgggcg gtgcccgcgc tccgactggt cctgccgccg ctgccgcagc tggcgccgga
     1381 tctctacctt ccgccggtcg tactcttcat cccgacggcg gcggaaatgg ccgcacccct
     1441 gccggcggaa gccggacccg ggcagtgggt gcccttcatg ctcgcaatgt gggccggggg
     1501 tgcggtcatc ttcctgacct tgcagtggct cgcctaccgc gccttcctag gccggataaa
     1561 ggagacctcc cggccggcgc ggccgcccct gttcggcggg atccggacct ggatcagcga
     1621 ctccgtcgac ggcccgctcg ccctgggcgt gatcgagcgg cggatcgtcc tgcccggcga
     1681 tttctcgagg cgctacaatc cggtcgagcg gcggctcgcg ctcgagcatg agctggtcca
     1741 ccacaagcgc ggcgacatct ggtggaacct gctggcgatg ctgatcctcg ccgtcttctg
     1801 gttcaatccc gtggcctggt gggccttccg ggccttccgc agcgaccagg agctggcctg
     1861 cgacgccgcc gtcgcccgca ccgcctcgct cgacgagcgg tgcgactatg cccgggccct
     1921 ggtcaagtcg gcgagccgcc ccggcctgat cgctgcctgc gcgctcaatc cggcgggaga
     1981 gctgaagcgg cgcctgcgaa tgatgcgcgc ccaccgcgcc agcccgctgc gctcggccgg
     2041 cggcgtcgcc gcgctcgccg ccttcgccgc cgccggcttc gcgctcggca gcgcccagcc
     2101 cgcc
//
LOCUS       DASVQZ010000251         6727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_628638, whole genome shotgun sequence.
ACCESSION   DASVQZ010000251 DASVQZ010000000
VERSION     DASVQZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628638"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1735)
                     /locus_tag="VF547_06800"
     CDS             complement(86..1735)
                     /locus_tag="VF547_06800"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8262564.1"
                     /translation="MLFKKLLAALAALCFAQAASADVLVDNVNGYTIGADGRLVRFGA
                     MLVGDDGKVERLYQRGERKAERPRYRFDGKGRTLLPGLIDAHGHVMSLGLNALQLDLS
                     DTRTLQEAQAKIAAYAAANPTPRWIVGRGWNQETWGLGRFPTAAELDSAVADRPVWLG
                     RVDGHAGWANSAAMKEAGITARTASPAGGRIEMARGQPTGIFVDAAAELIDKVVPPPL
                     PRTRDQALERAQQIMLANGLTAAADMGTSPDDWLVMRRAGDDGRLKVRIMAYGSGVDS
                     ALAIAGTRPTPWLYGDRLRLIGVKLYADGALGSRGAWLKQPYRDKPGERGLGFLGETE
                     LLNLMSRAAMDNFQVAVHAIGDAANAQVLAAIEEMSNTYKGDRRWRIEHAQIVDPADL
                     PRFARHGTIASMQPVHETSDWKMAEARMGLERLGGAYAWRSMIANRVPLAFGSDFPVE
                     SPNPFPGLAAAVSRQDPEGQPPGGWLPEQRLTLEQALAAFTRGAAYAGFAEDRVGSLE
                     KGRWADFILVDRDIFDVPTPRQLRETKVVETWIAGRKVWPN"
     gene            1793..3031
                     /locus_tag="VF547_06805"
     CDS             1793..3031
                     /locus_tag="VF547_06805"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX8262565.1"
                     /translation="MASPASASDLPVTLADIEAAARRIEGAVVHTPTVLSRTLSGATG
                     ADVWVKFENLQFTAAYKERGALNKLLLMEPATRSRGVIAASAGNHAQAVAYHGARLGV
                     PVTIVMPTSTPSIKVLQTEGHGAEIVLHGEMYDEAYAEALRLAEARALTFIHPFDDPE
                     IIAGQGTVALELLADAPEIDTLVVPIGGGGLISGVATAAKALRPDLRVVGVQAELYPS
                     MYCRISGDERPSAGDTLAEGIAVKKPGRLTPRIIERAVDEILLVSERYIETAVSLYLQ
                     IEKTVAEGAGAAGLAALLAHPERFSGRKVGLILSGGNIDTRLLATVLLRDLARSGRMA
                     RLRIQLQDRPGALLAVVRLFDRHGANIIEIYHQRVFTTLPAKDAFIDVECEARDSEHL
                     DALVRALEEAGFYVHPVEIR"
     gene            3158..3919
                     /locus_tag="VF547_06810"
     CDS             3158..3919
                     /locus_tag="VF547_06810"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX8262566.1"
                     /translation="MSAPFRFPRFFVTSPAPCPYLAGRTERKVFTELAGQHAADLNDA
                     LGRIGFRRSQAVAYRPSCVDCSACVSVRVVAGEFQPSTTQRRMLKRHGDLEVTACKPW
                     ATEEQYALLRRYLKRRHPDGGMVGMDESDFSDMIEQSPVKTYVIEYREPSRGGRPGKL
                     VGACLTDQQADGLSMIYSFFEPDDPARPGLGTYIILDHIVRAGKAGLPYVYLGYWVES
                     SRRMAYKARFRPLERLGPSGWHRFDPDQRELPLTS"
     gene            4054..6003
                     /locus_tag="VF547_06815"
     CDS             4054..6003
                     /locus_tag="VF547_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8262567.1"
                     /translation="MESVGGRILLEERFRALQRQIPLLYVVVLTNLVGLHLSCGGDIA
                     SPFVLMLTGLVVFRLAHWLRVRDRALPAERILRELRKTWVYSVIFSVGFCGWAFHLIA
                     TGDPNKENSVILFASLAAIGCAYGVSRYPAAARTALLLLGVPLALRLVFSDDIAHIGM
                     GASLGIVLLILMRLLSLHDEGFKELVESRTAIILERERAQRAERLAKAEKAKAKLIAD
                     TDPLTGLANRRAFLRALGRRAGGLARSGGGFALAMVDLDGFKPINDTFGHAAGDRVLE
                     GIGARLAAAAGEGALIARTGGDEFALLLPRIRSAAAATSVGIAICASLQEPFVVDGRE
                     FRISGCCGLTLLKLDDCDVDEALIRADTALYRAKEGGRSGVAVFSREMHEIHRRRILV
                     EKALRLPETLDRIGLVFQPIIDLATGELKALEALARWHDETLGQIPPDQFIPIAEQIN
                     VIEPISEKLLSLAAAEASRWASAVRLSFNLSAVQLCSAGSSGRILAILERGGLDPGRL
                     QIEVTETALLADFEVARENLHALRAAGARIVLDDFGAGHASISYLREMQFDGVKLDGA
                     LIASVVDSLRSRRLLKGVLNLCEALGLPSVAEHIETDEQLRLLRELGCREGQGYLLSA
                     PLLADAAAALAAPKLALIGSRAAAA"
     gene            complement(6183..>6727)
                     /locus_tag="VF547_06820"
     CDS             complement(6183..>6727)
                     /locus_tag="VF547_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX8262568.1"
                     /translation="IGDENALSVATAPSMVRVAELVGEAVQNVNRTVGSVLAEANISS
                     SVSMLLGGRIGAEPPSLYLIYSAGNFIHSSPESPFLQIGETKYGKPILDRGIRRGTPL
                     DEAVKVALLSFDSAMRSNLGVARPLDLVVMPADPARPIISRRIEPDDSYFNQLSLRWA
                     VLLHEATEQIPNPPWMEDPS"
BASE COUNT          998 a         2432 c         2243 g         1054 t
ORIGIN      
        1 gaggggttct ttcagactca gcaagcccct ccaccatgct tcgcatggtc cccctcccct
       61 gcaaatgcag gggaggaatt tcgcctcagt tcggccacac cttcctgccc gcgatccagg
      121 tctcgaccac cttcgtctcg cgcagctggc ggggcgttgg aacgtcgaag atgtcgcggt
      181 cgacgaggat gaagtcggcc cagcggccct tctccaggct cccgacccga tcctcggcga
      241 agcccgcata ggccgccccc cgagtgaacg ccgcgagcgc ctgctcaagc gtgaggcgct
      301 gctcgggaag ccagccgccg gggggctggc cttccgggtc ctggcggctg accgcggcgg
      361 cgaggccggg gaaggggttg ggcgattcga cggggaagtc ggagccgaag gcgagcggca
      421 cccggttggc gatcatgctc cgccaggcat aggcgccgcc gagccgctcg aggcccatcc
      481 gagcctcggc catcttccag tcgctggtct cgtgcaccgg ctgcatcgag gcgatggtgc
      541 cgtggcgggc gaagcgcggg aggtccgccg gatcgacgat ctgggcatgc tcgatccgcc
      601 agcggcggtc gcccttgtag gtgttcgaca tctcctcgat cgcggccagc acctgggcgt
      661 tcgcggcgtc gccgatggcg tgcacggcga cctggaaatt gtccatcgcc gcccggctca
      721 tcaggttgag cagctcggtt tcgccgagaa agccgaggcc gcgctcgccc ggcttgtcac
      781 gatagggctg cttgagccaa gccccgcgcg agccgagcgc gccgtcggca tagagcttca
      841 ctccgatcag ccggagccgg tcgccgtaga gccagggcgt cggccgggtg ccggcgatgg
      901 cgagcgcgct gtcgaccccg gatccatagg ccatgatcct gaccttcagc cgtccgtcgt
      961 cgccggcgcg gcgcatcacc agccagtcgt ccgggctggt gcccatgtcg gcggcggcgg
     1021 tgagcccgtt ggcgagcatg atctgctggg cgcgctcgag cgcctgatcg cgggtccggg
     1081 gcaggggcgg cggcaccacc ttgtcgatca gctcggcggc ggcgtcgacg aagattccgg
     1141 tcggctggcc gcgcgccatc tcgatccggc cgccggcggg ggaggcggtg cgggcggtga
     1201 ttcccgcctc cttcatcgcg gcgctgttgg cccagccggc atggccgtcg acgcgcccga
     1261 gccagaccgg acggtcggcg accgccgagt cgagctcggc cgcggtcggg aagcggccga
     1321 ggccccatgt ctcctggttc cagccgcggc cgacgatcca gcgcggcgtc gggttggcgg
     1381 cggcataggc ggcgatcttc gcctgcgcct cctgcagcgt cctggtgtcg gaaaggtcga
     1441 gctgcagcgc gttgaggccg agcgacatga cgtggccgtg cgcgtcgatc agtccgggca
     1501 ggagcgtccg gcccttgccg tcgaagcggt agcggggccg ctccgccttg cgctcgccgc
     1561 gctgatagag ccgctcgacc ttgccgtcgt cgccgaccag catcgctccg aaacggacca
     1621 gtcggccgtc ggcgccgatc gtgtagccgt tgacattgtc gacgaggacg tccgccgagg
     1681 cggcctgggc gaagcacaag gcggccaggg ccgcgagcag ctttttgaac agcatagggc
     1741 cgcgcataag gcttgtttcc cgggggcgcc agatggtcta gacgcagcgc ccatggccag
     1801 cccggcaagc gcttccgatc tccccgtcac gctcgccgac atcgaggccg ccgcccggcg
     1861 gatcgaaggg gcggtggtcc atacgccgac cgtcctcagc cgcaccctct ccggagcgac
     1921 cggcgccgac gtctgggtga agttcgagaa cctccagttc accgccgcct acaaggaacg
     1981 cggcgcgctc aacaaattgc tgctgatgga gccggcgacc cgctcgcgcg gggtgatcgc
     2041 cgcctcggcc ggcaaccatg cccaggcggt cgcctatcac ggcgcgcggc tcggcgttcc
     2101 ggtcaccatc gtcatgccga cctcgactcc gagcatcaag gtgctccaga ccgagggcca
     2161 cggcgccgag atcgtccttc acggggagat gtacgacgaa gcctatgccg aggcgctgcg
     2221 cctcgccgag gcgagggcgc tgaccttcat ccacccgttc gacgatcccg agatcatcgc
     2281 gggccagggc acggtcgcgc tcgagctgct cgccgatgcg ccggagatcg acaccctcgt
     2341 cgtgccgatc ggcggcggcg gcctgatctc gggcgtcgcc accgccgcca aggcgcttcg
     2401 gccggacctg agggtggtcg gcgtccaggc cgagctctat ccctccatgt actgccggat
     2461 cagcggcgac gagcgaccgt ccgccggcga caccctggcc gagggcatcg cggtcaagaa
     2521 gcccggccgc ctcacccccc ggatcatcga gcgcgcggtg gacgagatcc tgctcgtctc
     2581 cgagcgctat atcgagaccg ccgtcagcct ctacctccag atcgagaaga cggtggcgga
     2641 aggcgccggg gcggcgggcc tcgccgcgct gctcgcccat cccgagcgct tcagcggccg
     2701 caaggtcggc ctgatcctga gcggcggcaa tatcgacacc cgcctcctcg ccaccgtcct
     2761 gctccgcgac ctcgcccgct ccggccggat ggcccgcttg aggatccagc tccaggaccg
     2821 gcccggcgcc ctgctggcgg tggtgaggct gttcgaccgc cacggcgcca acatcatcga
     2881 gatctatcac cagcgggtgt tcacgacgct tccggccaag gacgcgttca tcgacgtcga
     2941 gtgcgaggcg cgcgactccg agcatctcga cgcgctcgtc agggcgctcg aggaggccgg
     3001 attctacgtc catccggtag agattcgctg atccttcgtc ccgtgccgga acaggcgcgg
     3061 ggttaactcc gttttggggg ctccttgccc ttcccaagcg gcgcgcaaag acgcataaac
     3121 cgagtttcaa ccacgcctct aaacgcctgg agccccgttg agcgccccct tccgctttcc
     3181 ccgcttcttc gtgacaagtc ccgcgccgtg cccctatctg gccggacgga ccgagcggaa
     3241 ggtgttcacc gagctcgccg gacagcacgc ggccgacctc aacgacgccc tcggccggat
     3301 cggcttccgg cggagccagg cggtcgccta ccgaccgagc tgcgtcgact gctcggcctg
     3361 cgtctcggtg cgggtcgtcg cgggcgaatt ccagccgagc acgacccagc ggcggatgct
     3421 caagcggcac ggcgacctcg aggtcaccgc ctgcaagccc tgggcgaccg aggagcaata
     3481 tgcgctgctt cgccgctacc tgaagcgccg ccacccggac ggcggcatgg tcggaatgga
     3541 cgagagcgac ttctccgaca tgatcgagca gtcgccggtc aagacctacg tcatcgaata
     3601 tcgcgagccg tccaggggcg gccgcccggg caagctggtc ggcgcgtgcc tcaccgacca
     3661 gcaggccgac ggtctgtcga tgatctacag cttcttcgag ccggacgatc ccgcccggcc
     3721 gggcctgggc acctacatca tcctcgatca catcgtccgc gccggcaagg cgggcctgcc
     3781 ctatgtctat ctcggctatt gggtcgagag ctcgcgccgg atggcctaca aggcccgctt
     3841 ccgcccgctc gagcgcctcg gcccgagcgg ttggcaccgc ttcgaccccg accagcgcga
     3901 gctgcccctg accagctgaa aatcctcccc cggcaattgc cggggaggaa cttacgcctg
     3961 ccccgccaac cacctcttaa tcatttccgc gtaatccgag cctccatggg gtgggcgata
     4021 aggcgactga tccggctgat tgacgccggc gaggtggaga gcgtcggggg gcgcatcctc
     4081 ctcgaagaac gcttccgggc gctccagcgc caaattccgc tcctctacgt cgtcgtcctc
     4141 accaatctcg tcggcctgca tctctcctgc ggcggggaca tcgcctcacc cttcgtcctg
     4201 atgctgaccg gtctggtcgt cttccgcctc gcgcactggc tccgcgtccg cgaccgcgcc
     4261 ctgccggccg aacggatcct ccgcgagctt cgcaagacct gggtctactc cgtcatcttc
     4321 agcgtcggct tctgcggctg ggccttccac ctgatcgcga ccggcgaccc gaacaaagag
     4381 aacagcgtca tcctcttcgc cagcctggcc gccatcggct gcgcctacgg cgtgagccgc
     4441 tatccggccg cggcacgcac cgccctgctc ctgctgggcg tgccgctggc gctccgcctc
     4501 gtcttctcgg acgacatcgc ccacataggc atgggcgcca gcctcggaat cgtccttctt
     4561 atcctgatgc ggctgctgag cctccacgac gagggcttca aggagctggt cgaatcgcgc
     4621 accgccatca tcctcgagcg ggaacgggcg caacgcgcgg agcgcctcgc caaggccgag
     4681 aaggccaagg ccaagctgat cgccgacacc gacccgctca ccggcctcgc caatcgccgc
     4741 gccttcctga gagcgctcgg ccgccgcgcc ggcggcctcg cccgaagcgg cggcggcttc
     4801 gctctggcga tggtcgacct cgacggtttc aagccgatca acgacacgtt cggccatgcg
     4861 gccggcgacc gggtgctcga ggggatcggc gcgcgcctcg ccgccgccgc cggggaaggc
     4921 gctctcatcg cccgcaccgg cggcgacgag ttcgccctgc ttctgccccg gatccgaagc
     4981 gcggccgctg cgaccagcgt cggaatcgcc atctgcgcgt cgctccagga accgttcgtg
     5041 gtggacggcc gcgagttccg gatctcgggc tgctgcggcc tcaccttgct caagctcgac
     5101 gattgcgacg tcgacgaggc gctgatccgg gccgacaccg cgctctaccg ggccaaggaa
     5161 ggcggccgct cgggagtggc cgtcttctcg cgcgagatgc acgagatcca ccgccgccgg
     5221 atcctggtcg agaaggcgct ccgccttccc gagacgctcg accgcatcgg gctcgtcttc
     5281 cagccgatca tcgacctcgc caccggcgag ctgaaggcgc tggaggcgct ggcccgctgg
     5341 catgacgaga cgctcggcca gatcccgccg gatcagttca ttccgatcgc cgagcagatc
     5401 aacgtgatcg agccgatcag cgagaagctg ctctccctgg cggccgccga ggcgtcgcgc
     5461 tgggcttcgg cggttcggct ctcgttcaac ctcagcgcgg tgcagctgtg cagcgccgga
     5521 tcgtcggggc gcatcctcgc catcctcgag cgcggcggcc tcgatccggg ccggctgcag
     5581 atcgaggtga ccgagaccgc cctgctcgcc gacttcgagg tcgcgcgcga gaacctccac
     5641 gcgcttcgcg ccgccggggc gaggatcgtg ctcgacgatt tcggcgccgg ccacgcctcg
     5701 atctcctatc tgcgcgagat gcagttcgac ggcgtcaagc tcgacggggc gctgatcgcc
     5761 agcgtcgtcg attcgcttcg aagccgccgt ctgctcaagg gcgtgctcaa cctttgcgag
     5821 gcgcttggcc tgccttcggt cgccgagcat atcgaaaccg acgagcagct ccgactgctg
     5881 cgcgagctcg gctgccgcga gggccagggc tatctgctct cggcccccct cctcgccgac
     5941 gccgccgccg ccctcgccgc ccccaagctc gccctcatcg gcagccgcgc ggcggcggcg
     6001 tagtaaattc ctcccctgca ttggcagggg agggggacca tgcgaagcag ggtggagggg
     6061 ccttgccgag ttcgaagaac ccctccaccg cgctgcgccg aaaaggcgca gtttatcctg
     6121 agcgccgccg caggcggcgt cgaagggcgc ggtccccctc cccgagaaag ctcggggagg
     6181 aattaggaag ggtcctccat ccacggcggg ttggggatct gctcggtcgc ctcgtgcagc
     6241 agaaccgccc agcgcagcga caattggttg aaataggaat cgtccggctc gatccggcgg
     6301 ctgatgatcg gccgggcggg atcggccggc atcaccacga ggtcgagcgg ccgagccacg
     6361 ccgaggttgg atcgcatcgc gctgtcgaac gacagcaggg cgaccttcac cgcttcgtcg
     6421 agcggggttc cgcgccggat gccgcggtcg aggatcggct tgccatattt ggtctcgccg
     6481 atctgcagga aaggcgattc gggcgaggaa tggatgaaat tgcccgccga gtagatcagg
     6541 tagagcgacg gcggctcggc tccgatccgc ccccccagca gcatcgacac cgagctcgag
     6601 atgttcgcct cggcgagcac cgatccgacc gtccggttga cgttctgaac cgcctcgccg
     6661 accagctccg cgacccggac catcgagggc gccgtcgcga cgctgagcgc attctcgtcg
     6721 ccgatcg
//
LOCUS       DASVQZ010000252         5563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_152603, whole genome shotgun sequence.
ACCESSION   DASVQZ010000252 DASVQZ010000000
VERSION     DASVQZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152603"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /gene="thiC"
                     /locus_tag="VF547_06825"
     CDS             complement(<1..1802)
                     /gene="thiC"
                     /locus_tag="VF547_06825"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX8262569.1"
                     /translation="MADAPAHSELLRVTTGPIRGSRKVHVGPLRVAMREIDLEPSSGE
                     PPLRVYDCSGPYTDPAASIDILRGLPELRRDWIRGRGDVEEVLQREVRPEDNGRLGPD
                     RSGGVAPFPNVRRKVLRARPGANVSQMHYARKGIVTPEMEYVAVRENLGRERMAAGAR
                     DGQDFGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNS
                     AVASDVAAEVDKMVWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALE
                     KVGGVAEDLTWEIYRDTLIEQAEQGVDYFTIHAGVRLPYIPMTAKRVTGIVSRGGSLM
                     AKWCLAHHKESFLYTRFEEICEVMKAYDIAFSLGDGLRPGSIADANDEAQFAELATLG
                     ELTQIAWRHDVQVMIEGPGHVPMHKIKANMDKQLEACGEAPFYTLGPLTTDIAPGYDH
                     ITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAA
                     KMRDDALSRARFEFRWRDQFHLSLDPDTAEQYHDQTLPAEGAKTAHFCSMCGPKFCSM
                     KITQEVRDFAAKQNASADTFLEADAGMAEMSEKF"
     regulatory      complement(1831..1938)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2034..2363
                     /locus_tag="VF547_06830"
     CDS             2034..2363
                     /locus_tag="VF547_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262570.1"
                     /translation="MRDWTRAMHIIEILLPLRDNEGRRFGAEAFARVREELVEHFGGL
                     TAFTRSPAEGLWEQGEGERSRDEIVIFEVMADWLDRGWWRDYRAQLEDRFRQDEIVVR
                     AREVELL"
     gene            complement(2517..3611)
                     /gene="dinB"
                     /locus_tag="VF547_06835"
     CDS             complement(2517..3611)
                     /gene="dinB"
                     /locus_tag="VF547_06835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8262571.1"
                     /translation="MNEAPAAAAIRKIIHVDMDAFYASVEQRDNPELRGKPLAVGGGV
                     RGVVAAASYEARKFGIRSAMPAVTARRRCPDLIFVKPRFDAYRAVSEQIRAIFRDYTP
                     HVEPLSLDEAYLDVTEDLKGIGVATLIAEEIRARIKAETGLTASAGVSYNKFIAKLAS
                     DQNKPDGLCVVTPARGPAFVEALPVKRFHGVGPKTAEKMARLGIETGADLKARSLDFL
                     AAHFGSSARYYHDLARGICHRQVRADRPYKSVSAEDTFLEDLGEPEALLSELERIGRH
                     VWTKRVVGKGLSGRTVNLKVKYADFQIVTRARSLDRPVSGEAEFLEIGAQLLRTLLPP
                     VKSVRLLGLGLSGLGEAECRDDGREPALPL"
     gene            complement(3756..4127)
                     /locus_tag="VF547_06840"
     CDS             complement(3756..4127)
                     /locus_tag="VF547_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262572.1"
                     /translation="MKGFVGLAVMGMMLVLASPATARDRPGEGGEARSFGTAPAASRS
                     ALVPQTVALDEKGLLPAVDPSAPRLFGSVPVADNVALGVGLFSVVGTTEKEQLRRRLD
                     PSREFLRSGTRVAAVGMSLRF"
     gene            4356..5450
                     /locus_tag="VF547_06845"
     CDS             4356..5450
                     /locus_tag="VF547_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX8262573.1"
                     /translation="MRRGKNCGLLIAGGGVAGCFAALAMARLRPEAPMVLVGEEVRFG
                     GGSGMLVLDAGLSDEERTLLGPVIARSWDACYVALPSRSRKLKLASHLVTTEAIHKAV
                     ADALPDRCARSAKVVAIRDDSLLLPGGETLAGTGAVDARGWAHQTTLEIAWRHRTART
                     CRFAEPHRVDLPVLADMTLETGGCGHFTCVPLDENTLRIERIDYSRSPEGGGDGTARI
                     EEYVGRRGWHDGEPRKAESSSRPIALGGDFAAYWRIGGARVAKLGARGGFAHPFTGSA
                     LPDALRTGLVLARQSDLGGSALHDLFESEAAALWKRREFHRGVNRLLLDKGRDALAAL
                     YDLDAPLLERFFAEDAGLFHRRKILAAVGG"
BASE COUNT          783 a         1885 c         2001 g          894 t
ORIGIN      
        1 ctgaacttct cgctcatctc ggccattccc gcgtccgcct cgaggaaggt gtcagcgctc
       61 gcattctgct tggcggcgaa gtcgcggacc tcctgggtga tcttcatcga gcagaatttc
      121 gggccgcaca tggagcagaa gtgggcggtc ttggcgcctt cggccgggag ggtctggtcg
      181 tgatattgct cggcggtgtc tgggtcgagg ctgaggtgga actggtcgcg ccagcggaat
      241 tcgaagcggg cgcggctgag ggcgtcgtcg cgcatcttcg ccgcggggtg gcccttggcg
      301 aggtcggcgg cgtgggcggc gagcttgtag gtgaccaccc ccaccttcac gtcgtcgcgg
      361 tcggggaggc cgagatgctc cttgggggtg acgtagcaga gcatcgcggt gccgaaccag
      421 ccgatcatcg cggcgccgat cgcgctggtg atgtggtcgt agcccggggc tatgtcggtg
      481 gtcaggggcc cgagcgtgta gaagggcgcc tcgccgcagg cttcgagctg cttgtccatg
      541 ttggccttga tcttgtgcat cggcacgtgg ccggggccct cgatcatcac ctggacgtcg
      601 tggcgccagg caatctgggt cagctcgccg agcgtcgcca gctcggcgaa ctgggcttcg
      661 tcattggcgt cggcgatgga gccggggcga aggccgtcgc cgaggctgaa ggcgatgtca
      721 taggccttca tcacctcgca gatctcctcg aagcgggtgt agaggaagct ctccttgtga
      781 tgggcgaggc accatttcgc catgagcgag ccgcccctcg acacgatgcc cgtcacccgc
      841 ttcgccgtca tcgggatgta ggggaggcgc acgccggcgt ggatggtgaa atagtcgacg
      901 ccctgctcgg cctgctcgat caaggtgtcg cggtagatct cccaggtcag gtcctcggcc
      961 acgccgccga ccttctcgag cgcctggtag atggggacgg tgccgatcgg caccgggctg
     1021 ttgcggagga tccattcgcg ggtgtcgtgg atgttgcgcc cggtcgacag gtccatgacc
     1081 gtgtcggcgc cccagcggat cgaccagacc atcttgtcga cctcggcggc gacgtcggag
     1141 gcgacggcgc tgttgccgat gttggcgttg atcttgacca ggaagttgcg gccgatcgcc
     1201 atcggctcgc tctcgggatg gttgatgttg gaggggatga tggcgcggcc gcgggcgacc
     1261 tcgtcgcgga cgaactcggg ggtgacatag tccgggatgg aggcgccgaa atcctggccg
     1321 tcgcgcgccc cggccgccat ccgctcgcgg ccgagattct cccggacggc gacatattcc
     1381 atctcggggg tgacgatccc cttgcgcgcg tagtgcatct ggctgacgtt cgcgcccggc
     1441 ctggcgcgga gcaccttgcg gcggacgttg gggaagggcg ccactccgcc cgagcggtcg
     1501 gggccgagcc ggccgttgtc ctcgggcctg acctcgcgct gcagcacctc ctcgacgtcg
     1561 ccgcggccgc ggatccagtc ccggcggagc tcgggcaggc ctctcaggat gtcgatgctc
     1621 gcggcggggt cggtataggg cccggagcag tcgtagaccc gcaagggcgg ctcgcccgag
     1681 ctcggctcga ggtcgatctc gcgcatcgcc acccggagcg gcccgacatg gaccttgcgg
     1741 ctgccgcgga tcgggccggt cgtgactctc agaagctcgc tgtgcgcggg ggcgtcggcc
     1801 atcttcgctc tcctcaaatg gaggaagggc ggatcttccg cgctccctcc ctacgccggt
     1861 gtcagccgga tcaggttcgg cgggtcatgg cttcagccaa atctcaaccg cttgggcgcg
     1921 gtcccccggg gatggacctc gtctaggcgc ttcggccccg ccgggaaagc gggaatccgg
     1981 gcccgcggcg cgaatcctcg ccgcctcggc ggggatgacg gggccggggc gatgtgcgag
     2041 actggacgcg cgccatgcac atcatcgaga tcctcctccc gctccgcgac aatgaggggc
     2101 ggcgcttcgg agcggaggcc ttcgcccgcg tccgcgagga gctggtcgag catttcggcg
     2161 gcctcaccgc cttcacccgg agcccggccg aaggcctgtg ggagcagggg gagggcgagc
     2221 gcagccgcga cgagatcgtg atcttcgagg tgatggccga ctggctcgac cgcggctggt
     2281 ggcgcgacta tcgggcgcag ctcgaggacc ggttccgcca ggacgagatc gtggtccggg
     2341 cccgcgaggt ggagctgctg tagagcagga ccgtctcgga ttgagccggt ccgctcatcc
     2401 tgagtagccg gtgagcttgt cgaaccggcg tatcgaagga cggtccttcg atacgggcct
     2461 tcgtcttcgc tcagtcccta ctcaggatga gcggtccagt cgtatagcat catcctctac
     2521 agcggcagcg ccggctcgcg tccgtcgtcg cggcattcgg cttcgccgag gccggagagg
     2581 ccgaggccga gcagccggac gctcttcacc ggcggaagca gcgtgcgcag cagctgggcg
     2641 ccgatctcga ggaactcggc ctcgccgctc accggccggt cgagcgagcg ggctcgggtg
     2701 acgatctgga aatcggcata tttgaccttg aggttgaccg tccggcccga gaggcccttg
     2761 ccgaccaccc gcttcgtcca gacgtggcgg ccgatccgct cgagctccga gagcagggcc
     2821 tccggctcgc ccaggtcctc gaggaaggtg tcctcggcgc tgaccgactt gtagggacgg
     2881 tcggccctga cctggcggtg gcagatgccg cgggcgagat cgtgataata gcgcgcgctg
     2941 gagccgaaat gggcggcgag gaaatcgagg ctgcgcgcct tcaggtcggc ccccgtctcg
     3001 atgccgagcc gggccatctt ctcggcggtc ttggggccga cgccgtggaa gcgcttgacc
     3061 ggcagggcct cgacgaaggc ggggccgcgg gccggggtga ccacgcacag gccgtcgggc
     3121 ttgttctgat cggaggcgag cttggcgatg aacttgttgt agctgacccc ggccgaggcg
     3181 gtgaggccgg tctcggcctt gatccgagct cgtatctcct cggcgatcaa ggtggcgacg
     3241 ccgatgccct tcaggtcctc ggtgacgtcg agataggctt cgtcgagcga gagcggctcg
     3301 acgtgcggcg tatagtcgcg gaagatcgcg cggatctgct cggagacggc gcgataggcg
     3361 tcgaagcgcg gcttgacgaa gatcaggtcg gggcagcggc ggcgcgcggt caccgccggc
     3421 atcgccgagc ggatcccgaa cttgcgggcc tcgtagctcg cggcggcgac gaccccgcgc
     3481 acccctccgc cgacggcgag cggcttgccg cgcagctccg gattgtcgcg ctgctcgacc
     3541 gacgcgtaga aggcgtccat gtcgacatgg atgatcttgc ggatcgccgc tgcggcgggc
     3601 gcctcgttca cgccctgttc ctagcgcaga cggaggccga ggaaaagcgc aaagcgcatc
     3661 ggcggcgatg ccggcgaagg ctggcctcca ggccgatgaa agcctcttcg ctcgtcaccc
     3721 ggcggggctc cggcctccgc cggggcgacg gcaactcaga agcgcaggct catcccgacc
     3781 gcggcgaccc gggtgcccga gcgcaggaac tcgcgcgacg gatcgagccg gcggcgcagc
     3841 tgctccttct cggtcgttcc gaccaccgag aagaggccga ccccgagcgc gacattgtcg
     3901 gccaccggaa cgctgccgaa caggcgcggc gccgacgggt cgaccgccgg caggaggcct
     3961 ttctcgtcca gtgcgacggt ctggggaacc agggcgctgc ggctcgcggc cggcgccgtc
     4021 ccgaagcttc ttgcctcgcc gccctctccg ggccggtccc gcgccgtcgc cggcgaagcc
     4081 aggaccagca tcatccccat cacggcgagc ccgacgaatc ctttcatggc gggctccgtt
     4141 ttctgctgtt cccccataca gcgcgcgact atgtcgcgaa gttccggttc tgtcacgcct
     4201 cgtcgcagcc ctgcctgccc tcgtcccgcc gaggggcgaa accgcggccg aaggcctccg
     4261 catcgaccaa aataccgtgc cgttatgggc ctttcggcac gcctcgccgc gcgcggggtt
     4321 ggcgtcgggg gccccgggac ggtaagcagc gagcgatgcg caggggcaaa aattgcgggc
     4381 ttctgatcgc gggcggcggc gtggcgggat gcttcgccgc gctcgccatg gcccggctga
     4441 ggccggaagc gccgatggtg ctggtcggcg aggaggtgcg gttcggcggc ggaagcggca
     4501 tgctcgtgct cgacgccggc ctctccgacg aggagcgcac gctgctcggc ccggtgatcg
     4561 cgagaagctg ggacgcctgc tacgtcgccc tcccctcgcg cagccgcaag ctcaagctcg
     4621 caagccacct cgtcaccacc gaggcgatcc acaaggccgt cgccgacgcc ttgcccgacc
     4681 gctgcgcccg ttcggccaag gtcgtcgcga tacgcgacga cagcctgctc ctgccgggcg
     4741 gcgaaacgct cgccggcacc ggcgccgtcg acgcgcgcgg ctgggcgcac cagacgaccc
     4801 tggagatcgc ctggcggcac cgcaccgccc gcacctgccg cttcgccgag ccccatcggg
     4861 tcgatctccc cgtcctggcc gacatgacgc tcgagaccgg cggctgcggc catttcacct
     4921 gcgtcccgct ggacgagaac acgctgcgca tcgagcggat cgattattcg cgcagccccg
     4981 aaggcggcgg cgacgggacg gcccggatcg aggaatatgt cgggcggcgc ggctggcacg
     5041 acggcgagcc gcgcaaggcc gaatcctcgt cccggccgat cgcgctcggc ggcgacttcg
     5101 ccgcctattg gcgaatcggc ggcgcccggg tcgcgaagct cggcgcgcgc ggcggcttcg
     5161 cccatccctt caccggctcg gccctgcccg acgcgctgcg caccggcctt gtcctcgccc
     5221 gccagagcga cctcggcggc tcggcgcttc acgacctgtt cgagagcgaa gccgcggcct
     5281 tgtggaagcg ccgcgagttc caccgcggcg tcaaccggct gctgctcgac aagggccgcg
     5341 acgcgctggc cgccctgtac gatctcgacg cgcccctgct cgagcgtttc ttcgccgagg
     5401 acgcggggct gttccaccgg cgcaagatcc tggcggcggt gggcggctag cccgcttcgc
     5461 atcgaccccc tccgccatcc gacgcacggg agacgagcct tggaatcgcc tcggccctgt
     5521 gctttagggc gcggccatga acgtccatga atatcaggcc aag
//
LOCUS       DASVQZ010000253         7691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_152810, whole genome shotgun sequence.
ACCESSION   DASVQZ010000253 DASVQZ010000000
VERSION     DASVQZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152810"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF547_06850"
     CDS             complement(<1..260)
                     /locus_tag="VF547_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262574.1"
                     /translation="MYHAPVPLDLDQQEIEPAELADESVGTDASSQRLRDAHLHNGQA
                     QTRLLFAPFFAVTGAGAALLVARAMLGTARIELIGGWLALVG"
     gene            complement(484..1083)
                     /locus_tag="VF547_06855"
     CDS             complement(484..1083)
                     /locus_tag="VF547_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX8262575.1"
                     /translation="MSRDPRIDAYIARQADFARPILDHLRAAVHSACPEAEETLKWGM
                     PHFLYKGQLLAGMAAFKAHATFGFWRAKEVLGETGAEREAMGQFGRLTSVSDLPPDDV
                     LAALVRQAMAVADAGTRPARPKAAPRAELETPPDLRAALDANPAARATFDSFPPSGRR
                     DYVQWVVEAKRPETRDKRIAQAVEWMAEGKRRHWKYENC"
     gene            complement(1132..4137)
                     /locus_tag="VF547_06860"
     CDS             complement(1132..4137)
                     /locus_tag="VF547_06860"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8262576.1"
                     /translation="MPDPSSVAFRLRAALLLILALLCAGPAAAAEPANPHLSALVRGV
                     DDDENRRETLRIGKLDISVHIHGGIADTILTARFVNPGEESLEADFGLALPAGSVVTG
                     YALDIEGEMIEGVLLDQRKARRAYQERVRQTVDPGLGEVLRGFQFRSRVSPVPPRSAR
                     TIRIRFSTPLDPKLGYVLPLSGGAEVEDFVLTVEAGGLSRQPAVTLPDGTGLRPDPGP
                     GPYRLSVARTQLRLRRALAISAVERAAPLLVSRHPSGDAFFELADTGTAQAASRGPRS
                     VAVLWDRSLSRADDDLAAEAALLRAYLERVRPERTELILFDSAGVDRIAIASPDGLAP
                     ALAAVRYRGGSSVAVLSPQAIDAEACLLFTDGLVTIDRREDWRPSCPLFAVSSAPDSD
                     RPWLAAVAAASGGQAVHLAGGNGAQLLDRLTGAVPRVLAVRTTAGTPIDYSLLDAPDA
                     GWRLVAPVPRSGGIVVRLAGPDGRSVERVYAMPAADMPRLTGPGALWASERLALRAAS
                     DDTDRDALVAFARRHRVSGPDISFLVLESPEDYARAGIEPPPGLAPEKRQTYERLVAQ
                     RRSEEQQQRDKRLETVLAAWEEQKAWWNQRFDPNAKPARPRDQDGNLAAPAPAPPPLV
                     EAVPPPPAPDVLDELPAAEASEGAAGDEGDITVTGTRISLPNLSSMSPVTVVGGSEIA
                     GQGRTTIEVAEWSADRPYLKALAAAEPAQLESVLAAQQAEHGGLPAFWLDVSDWYFRT
                     GRRADALRLLLSALDLPTRDSETVAIVAARLVRWGELDRAIRLYERLAAAESDRPQPL
                     RSLALALARRAEAAPPEQARADLDRAIALLTEVVMTEWPRAFEGIEMIALMEVNRLIA
                     RQSRLGGGPVPLDPRLVALLDVDLRVVIEWNTEETDLDLWVDEPNGERAIYSNPRTLI
                     GGRLSNDMTAGYGPEEYLLRRAPRGTYTVRANVFASDRLNPNGSSRITAHLIHDFGRA
                     SEREEVIDVEALPGAESGERLIGRLRVER"
     gene            4298..4534
                     /locus_tag="VF547_06865"
     CDS             4298..4534
                     /locus_tag="VF547_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX8262577.1"
                     /translation="MRAARLSSSVIARVAYDDEERALSIWFRETGRYIYSDVPKVIYE
                     GLCKAPSAGRYFNACIKRRFPCRPDPERRRFRPA"
     gene            complement(4544..5305)
                     /locus_tag="VF547_06870"
     CDS             complement(4544..5305)
                     /locus_tag="VF547_06870"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX8262578.1"
                     /translation="MAAILVVAGAGSAAAEEGKKPRRIVRVAVGPEIVPDHVGADGTK
                     FSPYVNVDLKREGKEFEFEAPDESFGFGLIDLGPFEFGPALNLESTRRPSRFPVPVDK
                     VPMTVEAGAFAQLYLAKSFRVRGELRKGLGGHDGWISHLSADFVTRRGDDYVFSIGPR
                     LTWADEEYQQAYFGVSPAMSAASGLPAFDADSGVRGIGGTTSISYQFSSDWGIQAYGR
                     YERLVGDAGRSPLIRTYGSRDQFAAGLALSFTFGL"
     gene            complement(5401..6558)
                     /locus_tag="VF547_06875"
     CDS             complement(5401..6558)
                     /locus_tag="VF547_06875"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8262579.1"
                     /translation="MPAPLLAPAPESELSLPGWIYRDPEFFEVEMERVIRPSWQVVCH
                     SGEIARPGEWRTIDYLGESVLVVRGDDGEVRAFSNVCRHRGSRVVSGGAGCARRLTCP
                     YHAWTYALDGRLIGVPHKGEYRGLEASRLGLVPVELEAWRGFLFVRLESGGPSVAEMM
                     APHEAEIAPYRFEQLKALGRVTLRPRDVNWKNVADNYSDGLHIAVAHPGLTRLFGRGY
                     AISAGAHVDRMAGDLVERPSANPSERAYQRFLPDVAHLPAELRRRWLYFKLWPNVAFD
                     IYPDQVDFMQFLPVSPTETVIREISYALPDGRREMKAARYLNWRINRRVNAEDTALIR
                     GVQQGMASRTYTPGPLGESEVCLRSFARRLRRLIPEANLHRPPAPGWSRGA"
     gene            6602..7006
                     /locus_tag="VF547_06880"
     CDS             6602..7006
                     /locus_tag="VF547_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8262580.1"
                     /translation="MRPELAVLAWGCILALVHVFVAVRFKTRQYGTKWNMGARDEELP
                     PPQPIVGRLARAQANFFETFPIFAAAALIVAVAGLGDRWTAIGAWLWLGARIVYLPLY
                     ALGVPVVRTVAFLVSVVGIALVLRPALAAALG"
     gene            7041..7265
                     /locus_tag="VF547_06885"
     CDS             7041..7265
                     /locus_tag="VF547_06885"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX8262581.1"
                     /translation="MVGRNFWVVGGESGWEVRQEGLPGETTRHATQEEAWAAANARAR
                     DCHGEVFLADGEGGHSDYACHRELPRKIKI"
     gene            complement(7262..>7691)
                     /locus_tag="VF547_06890"
     CDS             complement(7262..>7691)
                     /locus_tag="VF547_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262582.1"
                     /translation="MTGACAVNPSTPKGLYSPCRVLGSSDWKAHVEVVPNNTPVPYLR
                     RKLVVTGKVTTAGGGFYVSLDEGPVSRLDDPVQQILVRTEGSAEAGAAPVTHAVRGVF
                     PALDHYGGIAIRCGDGIIAEIKDVPIPPREKGEDPFRLKD"
BASE COUNT         1122 a         2690 c         2759 g         1120 t
ORIGIN      
        1 aagcccacca gcgcgagcca gccgccgatc agctcgatcc gcgccgtgcc gagcatcgcc
       61 cgggcgacga gcagggccgc tccggccccg gtgaccgcga agaacggcgc gaacaggagc
      121 cgggtctgcg cctggccgtt gtggagatgg gcgtcgcgca gccgctggct gctggcgtcg
      181 gtgccgacgc tctcgtccgc cagctccgcc ggttcgattt cctgctggtc gaggtccagg
      241 gggactggcg cgtgatacat gggaatcctg gtgctgccgg gttccccatt tccctagccg
      301 cagagttttg atggagtgtt aaggacgcga cggaatgcga gtccttcgaa acgggccgtc
      361 gatccgtcct tcgatacgcc ggttctacag gctcaccggc tgctcaggat gagcggtgga
      421 gaataggtcc gccaaccgct catcctgagt agggactgag cgtggcgaag gcccgtcgaa
      481 ggatcagcaa ttctcatatt tccagtggcg ccttttgcct tcggccatcc attcgaccgc
      541 ttgcgcgatg cgcttgtcgc gagtctccgg ccgcttcgcc tcgaccaccc actggacgta
      601 atcgcgccgc ccgctgggcg ggaaggagtc gaaggtggcg cgcgccgccg gattggcgtc
      661 cagcgcggcc ctcaggtcag gcggcgtctc gagctcggcc ctgggcgccg ccttgggacg
      721 ggccgggcgg gtcccggcgt cggcgaccgc catcgcctgg cggaccagcg ccgccagaac
      781 gtcgtccggc ggcaggtccg acaccgacgt caggcggccg aactgcccca tcgcctcgcg
      841 ctcggcgccg gtttcgccca gcacctcctt ggcgcgccag aagccgaagg tggcgtgcgc
      901 cttgaaggcg gccatgccgg cgaggagctg ccccttgtag aggaaatggg gcatgcccca
      961 cttcagcgtc tcctccgcct cggggcaggc cgagtggacg gcggcgcgca gatgatcgag
     1021 gatcggacgg gcgaaatcgg cctgccgggc gatataagcg tcgatgcgcg ggtcgcgaga
     1081 catgcccggc ttataacctc gttccgcggc cggatcgagc ggcccgcacc ctcagcgttc
     1141 gacgcgcagc ctgccgatca gccgctcgcc gctctcggcg ccgggcagcg cctcgacgtc
     1201 gatcacctcc tcgcgctcgc tcgcccgccc gaaatcgtgg atgagatggg cggtgatccg
     1261 cgacgacccg ttgggattga gccgatcgga cgcgaagacg ttcgcgcgga cggtgtaggt
     1321 gcctcgagga gcgcgtcgaa gcagatattc ttccggtccg tagccggcgg tcatgtcgtt
     1381 ggacagccgg ccgccgatga gggtccgggg gttgctgtag atcgcgcgct cgccgttcgg
     1441 ctcgtccacc cagaggtcga ggtccgtctc ctcggtattc cactcgatga ccacccgcag
     1501 atcgacgtcg agcagggcca cgaggcgggg atcgagcggc accgggccgc cgccgagccg
     1561 gctctgtctg gcgatgagcc ggttcacctc catcagcgcg atcatctcga ttccctcgaa
     1621 cgccctgggc cattcggtca tgaccacctc ggtcagcagc gctatggcgc ggtcgaggtc
     1681 ggcgcgggcc tgttccgggg gcgccgcctc ggcccgcctg gccagcgcca gggcgaggct
     1741 gcgaagcggc tgcggccggt cggactcggc cgccgcgagg cgctcgtaga gccggatggc
     1801 gcggtccagc tcgccccatc ggacgagcct cgccgccacg atcgccaccg tctcgctgtc
     1861 gcgcgtcggc aggtcgagcg cggacagcag gagccggagc gcgtcggcgc gccggccggt
     1921 gcggaaatac cagtcggaaa cgtccagcca gaaggcggga aggccgccgt gctcggcctg
     1981 ctgcgccgcg aggaccgact cgagctgcgc gggctcggcg gcggcgagcg ccttcagata
     2041 cggccggtcg gcggaccatt ccgcgacctc gatggtggtc cggccttggc cggcgatctc
     2101 gctgccgccg acgacggtga ccggactcat cgaactgagg ttggggagcg agatccgcgt
     2161 cccggtgacg gtgatgtcgc cctcgtcgcc agcggcgccc tcggacgcct ccgcggcggg
     2221 caattcgtcg aggacgtcgg gcgccggcgg cggcggcacc gcctcgacga gcggaggggg
     2281 cgccggagcc ggcgcggcca ggttgccgtc ctggtcgcgg ggccgcgcgg gcttggcgtt
     2341 gggatcgaag cgctggttcc accaggcttt ctgctcctcc caggccgcga ggacggtctc
     2401 gagcctcttg tcgcgctgct gctgctcctc gcttctgcgc tgggcgacga gccgctcgta
     2461 cgtctgccgc ttctccggcg cgagccccgg cggaggctcg atgccggcgc gggcatagtc
     2521 ttcgggcgac tcgaggacca ggaaggagat gtcgggccct gagacccgat gccggcgggc
     2581 gaacgcgacc agagcgtcgc ggtcggtgtc gtcgctggcg gcgcgcaggg cgaggcgctc
     2641 ggacgcccag agggccccgg gcccggtcag gcgcggcatg tcggcggcgg gcatggcgta
     2701 gactcgctcg acgctgcggc cgtccggccc ggcgagccgg acgacgatcc cgccgctgcg
     2761 ggggaccggc gcgaccagcc gccagccggc gtccggagcg tcgagcaggc tgtagtcgat
     2821 gggagtgccg gcggtggttc ggactgcgag gacccgcggc accgcccccg tcagccggtc
     2881 cagcagctgc gcgccgttgc cgccggccag atggaccgcc tgccccccgg aggcggcagc
     2941 gacggcggcg agccagggtc ggtcggagtc gggcgcgctc gagacggcga agagtgggca
     3001 ggaaggccgc caatcctcgc ggcgatcgat cgtcacgagg ccgtcggtga acagcaggca
     3061 ggcctccgca tcgatggctt gcggcgacag caccgcgacg ctcgacccgc cgcggtagcg
     3121 gaccgccgcc agcgccggag cgagcccgtc gggcgaggct atcgcgattc ggtcgacgcc
     3181 cgcgctgtcg aaaaggatca gctcggtgcg ttccggccgc acccgctcga gataggcccg
     3241 aagcaaggcc gcctcggcgg cgaggtcgtc gtcggcccgc gacagcgacc gatcccaaag
     3301 caccgcgacg gatcgcggcc cgcgcgaagc ggcctgcgcc gtcccggtat cggcgagctc
     3361 gaaaaaggcg tctccgctcg gatggcgcga caccagcagc ggcgcggcgc gttcgacggc
     3421 cgagatggcg agcgcgcggc gcagccgcag ctgcgtccgg gcgaccgaga gccggtacgg
     3481 gcccggaccg gggtcggggc gcagcccggt cccatcgggg agggtcacgg cgggctggcg
     3541 ggaaaggccg cccgcctcga cggtcaggac gaagtcttcg acctcggctc cgccggagag
     3601 cggcagcaca tagccgagct tcggatccag cggagtgctg aacctgatcc tgatcgtccg
     3661 cgcgctccgc ggcggcaccg ggctgacccg cgaccggaac tggaagccgc ggagcacctc
     3721 gccgaggccc ggatcgaccg tctggcgaac ccgctcctga taggcccgcc gcgccttgcg
     3781 ctggtcgaga agcacgcctt cgatcatctc cccttcgatg tcgagcgcgt agcccgtgac
     3841 gaccgacccc gccgggagcg ccagcccgaa atcggcctcc aggctctcct cgcccggatt
     3901 gacgaagcgg gcggtcagga tcgtgtcggc gattccgccg tggatgtgca cggagatatc
     3961 gagcttgccg atgcgcaggg tctcgcgccg gttttcgtcg tcgtccacgc ccctcaccag
     4021 cgccgacaaa tgcgggttcg ccggttcggc ggcggcggcc ggaccggcgc acagcagggc
     4081 gaggatcagg agcagagccg ctcgcaggcg gaatgcgacg ctggacgggt caggcatgtc
     4141 tcatcccctt tcggacagcc tagcggccgc tcgccggcac gcaagggcgg cgggagactc
     4201 gcctcggcgc ttattcgatt cggtccggcg aaccggccgg aaccggcgac tcgccgatgc
     4261 gctggcgcgc gattctcctc ggtcagggac gagaatcgtg cgcgccgcca gattgtcctc
     4321 cagcgtcatc gcccgggtcg cctatgacga cgaggagagg gcgctgagca tctggttccg
     4381 cgagaccggg cgctacatct attcggacgt gccgaaggtg atctacgaag gcctgtgcaa
     4441 ggcgccctcg gccggccgct atttcaacgc ctgcatcaag cgccgcttcc cctgccgacc
     4501 ggatccggag cgccgcaggt tccggccggc gtgaccggcc cgcctacaag ccgaacgtga
     4561 agctgagcgc caggcccgcg gcgaactggt cgcgcgagcc gtaggtgcgg atcagcgggg
     4621 agcggccggc gtcgccgacc agccgctcgt agcggccata ggcctggatg ccccagtcgg
     4681 agctgaactg gtagctgatc gacgtcgtgc ccccgatccc gcgcacgccg gaatcggcat
     4741 cgaaggcggg caggccgctc gcggcggaca tggccgggct cactccgaaa taggcctgct
     4801 gatattcctc gtccgcccag gtgagcctgg ggccgatcga gaagacatag tcgtcccccc
     4861 gccgggtgac gaagtcggcg gagaggtgcg agatccagcc gtcgtgcccg ccaaggccct
     4921 tgcgcagctc cccccgcacc cggaacgact tggcgaggta gagctgggcg aaagcgccgg
     4981 cttcgacggt catcggtacc ttgtcgacgg gcaccgggaa gcgcgagggc cgccgcgtgc
     5041 tctccaggtt cagcgccggg ccgaactcga acgggccgag gtcgatcagg ccgaagccga
     5101 agctctcgtc gggcgcctcg aactcgaact ccttgccctc gcgcttgagg tcgacgttca
     5161 cgtagggcga gaatttggtg ccgtcggcgc cgacatggtc cggcacgatc tcgggcccga
     5221 ccgccacgcg cacgatccgg cgcggcttct tcccctcctc cgccgccgcg ctgccggcgc
     5281 ctgccaccac gagaattgcc gccaaggccg atttgtaccg cattgtccga tgactcccgc
     5341 cgaccacgaa taggaagcgc tacgaatgac cgccgcagcc gttccgtcaa gggggaccgg
     5401 ctacgcgccc cggctccaac ccggcgccgg cgggcggtgc aggttcgctt cggggatcag
     5461 ccgcctgagc ctgcgcgcga agcttctgag gcacacttcg ctctccccaa ggggtcccgg
     5521 cgtgtaggtg cgcgaggcca tgccctgctg gacgccgcgg atcagcgccg tgtcctcggc
     5581 gttgacccgg cggttgatcc tccagttgag gtagcgagcc gccttcatct cgcgccgccc
     5641 gtccggcagc gcgtagctga tctcgcggat caccgtctcg gtcggcgaga ccggcaggaa
     5701 ctgcatgaaa tcgacctgat cgggatagat gtcgaaggcg acgttcggcc acagcttgaa
     5761 atagagccag cgccggcgaa gctcggcggg caggtgcgcc acgtcgggca ggaagcgctg
     5821 gtaagcccgt tcggacggat tggccgaagg ccgctcgacc aggtctccgg ccatccggtc
     5881 gacgtgcgcg ccggccgaga tggcgtagcc ccggccgaac aggcgggtga ggccgggatg
     5941 ggcgacggcg atgtgcaggc cgtccgaata attgtcggcg acgttcttcc agttgacgtc
     6001 gcgcggccga agcgtgactc gcccgagcgc tttcagctgt tcgaagcgat agggcgcgat
     6061 ctcggcctcg tgcggcgcca tcatctcggc gacgctcggg ccgccgctct cgagccgcac
     6121 gaacagaaag ccgcgccaag cctccagctc gaccgggacg aggccgagcc gggaagcttc
     6181 cagcccgcga tattccccct tgtgcgggac gccgatcagc cggccgtcca gggcataggt
     6241 ccaggcgtga tagggacagg tcagccgtcg ggcgcagccc gccccgccgc tcaccacccg
     6301 cgagccgcgg tggcggcaga cgttggagaa ggcgcgcacc tcgccgtcgt cgccgcgcac
     6361 gaccagcacg ctctcgccga gatagtcgat cgtccgccac tcgcccggcc gagcgatctc
     6421 gccggagtgg cagaccacct gccaagaggg ccggatcacg cgctccatct cgacctcgaa
     6481 gaattcgggg tcgcggtaga tccagccggg caggctgagc tcggattccg gcgccggagc
     6541 gagaagggga gcgggcatgg cgcaccttgg caaagcccgc cctttttgtc accctcgcgc
     6601 gatgagaccc gaactcgccg tcctcgcctg gggctgcatc ctcgccctcg tccacgtctt
     6661 cgtcgcggtt cgcttcaaga cgcggcaata tgggacgaaa tggaacatgg gcgcgcgcga
     6721 cgaggagctg ccgccgccgc agccgatcgt cggccgtctc gcccgcgccc aggccaattt
     6781 cttcgagacc ttcccgatct tcgcggccgc cgcgctgatc gtcgcggttg ccggcctcgg
     6841 cgaccgctgg acggcgatcg gcgcctggct gtggctgggc gcccggatcg tctaccttcc
     6901 gctctacgcg ctcggtgtgc cggtggttcg gacggtcgcc ttcctggtca gcgtcgtcgg
     6961 tatcgccctg gtgcttcggc cggcgctcgc ggctgccctg ggctgaggca accgcggccg
     7021 ccgccggccg ttctagccgc atggtcggca ggaacttctg ggtcgtcggc ggcgaatcgg
     7081 gctgggaagt ccgccaggag ggcctccccg gcgagaccac gcgccatgcc acgcaggagg
     7141 aggcctgggc cgccgccaac gcccgggcgc gcgactgcca cggcgaggtc ttcctcgcgg
     7201 acggcgaagg cgggcacagc gactatgcct gccaccgcga gctgccgcgc aagatcaaga
     7261 tctagtcctt caagcggaac ggatcttcgc ccttctcccg cggcgggatc ggcacgtcct
     7321 tgatctcggc gatgatcccg tcgccgcagc ggatcgcgat tccgccataa tggtcgagcg
     7381 ccgggaacac gccgcggaca gcgtgggtga ccggcgcggc gcccgcctcg gccgagccct
     7441 cggtgcgcac caggatctgc tggacgggat cgtcgagcct cgacaccggg ccttcgtcga
     7501 ggctgacgta gaagccgccg cccgcggtgg tcaccttgcc ggtgacgacg agcttgcgcc
     7561 gcagataggg caccggggtg ttgttcggga cgacttcgac atgggccttc cagtccgacg
     7621 atcccagcac ccggcagggc gaatagaggc ccttcggcgt ggacgggttg acggcgcagg
     7681 cgccggtcat g
//
LOCUS       DASVQZ010000254         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_437420, whole genome shotgun sequence.
ACCESSION   DASVQZ010000254 DASVQZ010000000
VERSION     DASVQZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437420"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1286
                     /locus_tag="VF547_06895"
     CDS             330..1286
                     /locus_tag="VF547_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8262583.1"
                     /translation="MSPASEIFRSLADPTRLRILALLRAMELSVGELAQVLGQSQPRV
                     SRHVRILIEAGLAERRKEGSWVFLSLADPGRVQPLFAALERWAEIDGEDPWAAADAAR
                     LAAVRADRAAAAERYFAAHAGNWDEIRSLHIAECEVEAACRRALAGRPVGRLVDIGTG
                     TGRMLELFGADADRALGVDRSPEMLRLARVKLSEAGLAAAELRQGDMYALPLAAGSAD
                     TVILHQVLHYAQQPAAAVAEAARLLAAGGRLLIVDFAPHEREELRARDAHLRLGFADE
                     AMLKYLDGAGLKGRVVEHLEGGELTVTLWEGERPQEKLKVVA"
     gene            1283..2215
                     /gene="metF"
                     /locus_tag="VF547_06900"
     CDS             1283..2215
                     /gene="metF"
                     /locus_tag="VF547_06900"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX8262584.1"
                     /translation="MSPLAPNIARWREPLFADVAGDIGVSFEFFPPKTEKMEETLWEA
                     VKTLEPLAPRFVSVTYGAGGSTRERTHNTVVRIQQETPLNAAAHLTCVEASRSEIDEV
                     ALGYWQAGIRHVVALRGDPPEPGRGFQARPDGYANAAELVAGLKRVAPFEISVAAYPE
                     MHPDSATRAADLDNLKRKIDAGADRAITQFFFSPDCFFRFRDDAAAAGIDAELVPGIL
                     PVSNVAQTRKFAAMCGAAIPPWMDKLFEGLDDLPAARQLVAATVAAELCGQLYAGGIR
                     QFHFYTLNRAELAYAICHLLGLRPKEENERRTAA"
     gene            2193..3227
                     /locus_tag="VF547_06905"
     CDS             2193..3227
                     /locus_tag="VF547_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX8262585.1"
                     /translation="MNVAQQLRDAAAERILVKDGAYGTQVQARRLREADYAGTLGLSR
                     DQKGNNDLLNLTQPELIRDICQSFADAGAEILATNSFNANRISQADYGAEDLVREINC
                     AAARIIADVCEREAQKDGRKRFVAGALGPTNKTLSLSPNVNDPGYREIDFDTLSDVYV
                     EQIEGLVEGGAEIVLIETVFDTLNAKAAIHAARRVDRDLPIMLSMTITDLSGRNLSGH
                     SIEAFWASVRHARPLSIGLNCSFGASQLRAHVAALSAVADTLIMAYPNAGLPNDLGEY
                     DEGPAETAAQVRQWAEQGIVNIVGGCCGTTPAHIAAIAEAVKGFAPRKIAEPARRTLL
                     AGIEPMILAA"
BASE COUNT          491 a         1202 c         1048 g          503 t
ORIGIN      
        1 cgcgttgccg acgtcgccat tggcttcgtt ggtcgtctcc gactcgttgg cggcgctgtt
       61 gttggcttcg ttgctgccgg agcaggcggc gaggccgagc gtggcgactg caaccatggt
      121 gagggcgatc ttcttcacgg catgttcctt cgtagtttgc gaggagaacc agtgtgtccg
      181 tctgctgggc gaggtgccga aggttcccgt tccggcgttc cagcagggga aatgtacagg
      241 cgtgaaagga gaatgtctac tgtatcagtc aacaatacgc ctcaccgctt cgacttgacg
      301 gatataaaga tatctttatg tccgcaaaca tgtcccccgc ctcggaaatc tttcgctcgc
      361 tcgccgatcc gacgcggctg cgcattctcg cgctgctacg ggcgatggag ctgtccgtgg
      421 gcgagctggc ccaggtcctc gggcagagcc agccgcgggt ctcgcgccac gtccgaatcc
      481 tgatcgaggc cggccttgct gagcgccgca aggagggcag ctgggtgttc ctcagcctcg
      541 ccgatcccgg ccgggtccag cccctgttcg ccgccctgga gcgctgggcc gagatcgacg
      601 gcgaggatcc ctgggccgcg gccgacgcgg cccggctcgc cgccgtcagg gccgaccggg
      661 ccgccgccgc cgagcgctat ttcgccgccc atgccggcaa ttgggacgag atccgctcgc
      721 tccacatcgc cgagtgcgag gtcgaggccg cctgccgccg ggcgctcgcc ggccgcccgg
      781 tcggccggct ggtcgacatc ggcaccggca ccggccggat gctcgagctg ttcggcgccg
      841 acgccgatcg ggcgctcggc gtcgaccgct cgcccgagat gctccgcctc gcccgcgtca
      901 agctcagcga ggccggcctc gccgcggccg agctgaggca gggcgacatg tacgctttgc
      961 ccctggccgc gggctcggct gacacggtga tcctccacca ggtcctccat tacgcccagc
     1021 agcccgccgc cgcggtcgcc gaggcggcgc gcctgctggc ggccggcggc cggctcctga
     1081 tcgtcgattt cgccccgcac gagcgcgagg agctgcgcgc ccgcgacgcc catctgcgcc
     1141 tcggcttcgc cgacgaggcg atgctcaaat atctcgacgg cgccggcctc aagggccgcg
     1201 tcgtcgagca tctggaaggc ggcgagctca ccgtgacctt gtgggaaggc gagcggccgc
     1261 aggagaagct gaaggtggtc gcatgagccc gctcgccccc aacatcgccc gctggcgcga
     1321 gccgctcttc gccgacgtcg ccggcgacat cggggtctcg ttcgaattct tcccgcccaa
     1381 gacggagaag atggaggaaa cgctctggga ggcggtgaag acgctcgagc cgctcgcccc
     1441 gcgcttcgtc tcggtcacct acggcgccgg cggctcgacc cgcgagcgca cgcacaacac
     1501 ggtcgtgcgg atccagcagg agactcccct caacgccgcc gcccacctca cctgcgtcga
     1561 ggcgagccgg agcgagatcg acgaggtcgc gctcggctat tggcaggcgg gcatccgcca
     1621 cgtcgtcgcc cttcgcggcg atccgccgga gccgggcagg ggcttccagg cgcggcccga
     1681 cggctatgcc aacgccgccg agctggtcgc cggcctgaag cgcgtcgccc cgttcgagat
     1741 ctcggtcgcc gcctatcccg agatgcaccc cgattccgcg acccgcgctg ccgatctcga
     1801 caatctcaag cgcaagatcg acgcgggcgc ggaccgcgcc atcacccagt tcttcttctc
     1861 gcccgactgc ttcttccgct tccgcgacga cgcggcggcg gcgggaatcg acgccgagct
     1921 cgttcccggc atccttcccg tctccaacgt cgcccagacc cgcaagttcg ccgccatgtg
     1981 cggcgccgcg atcccgccct ggatggacaa gctgttcgag ggcctcgacg atctgccggc
     2041 cgcccgccag ctggtcgccg ccaccgtcgc cgccgagctt tgcggccagc tctatgccgg
     2101 cggaatccgc cagttccact tctacaccct gaaccgagcc gagctggcct acgccatctg
     2161 ccatttgctc ggcctccgtc cgaaggaaga aaatgaacgt cgcacagcag cttagggacg
     2221 ccgccgccga gcgtatcctg gtcaaggacg gcgcctatgg cacccaggtc caggcgcggc
     2281 ggctgcgcga ggccgattat gccggcacgc tcggcctgtc gcgcgaccag aagggcaata
     2341 acgacctcct gaacctgacc cagcccgagc tgatccgcga catctgccag agcttcgccg
     2401 acgccggcgc cgagatcctc gccaccaaca gcttcaacgc caaccggatc agccaggccg
     2461 attacggcgc cgaggatctg gtccgcgaga tcaactgcgc ggccgcccgg atcatcgccg
     2521 acgtctgcga gcgcgaggcg cagaaggacg gccgcaagcg cttcgtcgcc ggggcgctcg
     2581 gcccgaccaa caagaccctg tcgctctcgc ccaacgtcaa cgatcccggc tatcgcgaga
     2641 tcgacttcga caccctctcc gacgtctatg tcgagcagat cgaggggctg gtcgagggcg
     2701 gcgccgagat cgtcctcatc gagacggtgt tcgacaccct caacgccaag gcggcgatcc
     2761 acgccgcccg ccgggtcgac cgcgaccttc ctatcatgct ctcgatgacg atcaccgacc
     2821 tgtcgggccg aaacctctcc ggccattcga tcgaggcctt ctgggcgagc gtccggcacg
     2881 cccggccgct gtcgatcggg ctcaactgct cgttcggcgc aagccagctg cgcgcccatg
     2941 tcgcggcgct ctcggcggtc gccgacacgc tgatcatggc ctatcccaac gccggcctgc
     3001 ccaacgatct gggcgaatat gacgagggcc cggccgagac cgcggcccag gtccgccaat
     3061 gggccgagca gggcatcgtc aacatcgtcg gcggctgttg cgggacgacc cccgcgcata
     3121 tcgcggcgat cgccgaggcg gtgaagggct tcgcgccccg caagatcgcc gagcctgccc
     3181 gccgcaccct gctggcgggc atcgaaccca tgatcctcgc cgcctgagcc ctctcccctt
     3241 cagg
//
LOCUS       DASVQZ010000255         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_51836, whole genome shotgun sequence.
ACCESSION   DASVQZ010000255 DASVQZ010000000
VERSION     DASVQZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51836"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VF547_06910"
     CDS             complement(<1..105)
                     /locus_tag="VF547_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX8262586.1"
                     /translation="MRVLIVEDEPNLGRQLRATLEGAGYAVDLATDGED"
     gene            complement(235..537)
                     /locus_tag="VF547_06915"
     CDS             complement(235..537)
                     /locus_tag="VF547_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262587.1"
                     /translation="MRPLAALLVAVLGMTAVAPAAAGPREREQAEAFKGARDGRIVPL
                     RVIEGRIVPQMRGFSYLGPEYYADIGRYRLKFLRGQRVVWVDVDARTGEVIGRSGF"
     gene            700..1185
                     /locus_tag="VF547_06920"
     CDS             700..1185
                     /locus_tag="VF547_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262588.1"
                     /translation="MSGRRRAATGLAAALLAGCAGRPAPAPPPAPAAAPPPAAVLVDP
                     TPAATAPDWTLSPLTPGEWSYAGEAGGSEARFAGFGLRCDPGRREVIVSGPAGGRLRV
                     RTSYGERTLAAGAALPAADPLLDEMAFSRGRFAVETEGLETLVVPAWPEPARVIEDCR
                     G"
     gene            1521..1664
                     /locus_tag="VF547_06925"
     CDS             1521..1664
                     /locus_tag="VF547_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262589.1"
                     /translation="MTGNLPADQGRAPHLYDEQLQRQQQEKMLMRGRREGRPDLLNLP
                     PHR"
     gene            complement(1661..>1801)
                     /locus_tag="VF547_06930"
     CDS             complement(1661..>1801)
                     /locus_tag="VF547_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8262590.1"
                     /translation="TSEHRKAELPRWLHQYNWHRPHGSLQTQTPISRLGLDENNLLRL
                     HI"
BASE COUNT          254 a          610 c          652 g          285 t
ORIGIN      
        1 gtcctcgccg tcggtggcga gatccaccgc atagccggcg ccttccagag tcgcccgcag
       61 ctggcggccc agattgggct cgtcctcgac gatcagaacc cgcatcgcac tcccctctgg
      121 ctgtgtcggc gcccccttag cggcccaact ggcattgtgg ccaattgttt cactcccgtc
      181 atcccggcct tcgccgggac caggggctcg agtctcgagg ggaccgcgcg gcggtcagaa
      241 gcccgagcgg ccgatcacct cgccggtgcg ggcgtcgacg tcgacccaca cgacccgctg
      301 cccgcgcagg aacttgaggc gatagcggcc gatatcggcg taatattcgg gtccgaggta
      361 ggagaagccg cgcatctgcg ggacgatccg gccctctatg acccgaagcg gcacgattcg
      421 gccgtcgcgc gcgcccttga acgcctccgc ctgctcgcgc tcgcgcggac ccgccgccgc
      481 gggagcgacg gcggtcatgc cgaggacggc gacgagaagg gcggcgaggg gacgcatgga
      541 ggcactgctt ttctgctgtt aggcccgtgg catagcgtcg gcccattgaa tagcccatga
      601 atgcgcccgt cagccgaggg tcattcgccg gagaggcgaa aatgcccgca cggtcggttt
      661 cggcttggct tggccgcccg gccgtgctga gggcgcgcca tgagtgggcg gcgacgagcg
      721 gcgacgggat tggcggcggc cctgctggcc ggctgcgcgg gcaggccggc gccggcgccg
      781 ccccccgcgc ccgccgccgc gccgccgccg gcggccgtcc tcgtcgaccc gacacccgcg
      841 gccacggcgc cggactggac cctgagcccg ctgacgccgg gcgaatggag ctatgccggc
      901 gaggcgggcg gcagcgaggc gcgcttcgcc ggcttcggcc tgcgctgcga ccccggccgc
      961 cgcgaggtga tcgtgtcggg cccggcgggc gggcggcttc gggtccgcac cagctatggc
     1021 gagcggacgc ttgcggccgg agcggcgctg ccggcagccg accccctgct ggacgagatg
     1081 gcgttcagcc gcggccgctt cgcggtcgag acggaggggc ttgagaccct ggtcgtgccc
     1141 gcctggccgg agccggcccg ggtgatcgag gactgccgcg ggtgagatgt tcacgccgcg
     1201 ctctcggggc gcgcgcgaag cggaaagact agcggcgaaa aagatcaaaa caagtcttgc
     1261 gtggcgattc gcgatgattc atcctcgttt tcgccacaac aagcgaaagg aggtgatccg
     1321 atgtctcatg gttcagcaga gaggtcggtt ccgctcgttc gggagacgaa ggcctagcct
     1381 tccggtcgtc ttccgggctg gagcatccag ccgctgacca tgttcggagg gtcgtcgcgc
     1441 gaggcgcggc ggcccttcaa cttattcggt tcccgggccg gcgggcagga acggcgccag
     1501 cggccgcgcg ttttgccgtc atgaccggca atctcccagc ggaccagggc cgcgctcccc
     1561 atctctacga cgagcagctc cagcgccagc agcaggagaa gatgctgatg cgcggtcggc
     1621 gcgagggccg gcccgatctc ctcaacttgc ctccccatcg ctagatgtgg agcctcaaca
     1681 ggttgttctc gtccaggccg agtcggctga tgggtgtttg ggtttgtagg ctgccgtgtg
     1741 gccggtgcca gttatattgg tggagccatc tgggcagctc ggccttgcgg tgttccgagg
     1801 t
//
LOCUS       DASVQZ010000256        12708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_247788, whole genome shotgun sequence.
ACCESSION   DASVQZ010000256 DASVQZ010000000
VERSION     DASVQZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247788"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VF547_06935"
     CDS             complement(<1..315)
                     /locus_tag="VF547_06935"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX8262591.1"
                     /translation="MKSSVLFVCLGNICRSPLAEAACRRACERLGLEVEIDSAGTGDW
                     HIGYPPDPRAAAVAARNGVDISHLKARQVTSDDFRRFDHIVALDSTNLRDLERLRPAD
                     GKA"
     gene            386..2008
                     /locus_tag="VF547_06940"
     CDS             386..2008
                     /locus_tag="VF547_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8262592.1"
                     /translation="MAANPEATAPAASPPVRVGAYAWYALIVLVLVYALNFIDRQILS
                     ILAQDIKRDLGLEDAQIGFLYGTAFAVFYALFGIPLGRLADSWYRGRVMAIGLALWSS
                     MTALSGFASSFTMLAAARIGVGVGEAAASPAAYSMISDWFPKEKRATALSIYSSGLYI
                     GGALSLPIGGFVLSRWNLAFPVPSEAPLGLVGWQAAFLAVGIPGLLIALWVLSLREPQ
                     RGASDGLPQPIVRPHAWRDFGRELVAILPPLTLINVSRLPGNAMNLNLIVLGIVAVCA
                     LGLVRLTGDVPQWSAYALGVYAVFSWVQSLKHRDPATHRLIWGTPMTIVLAISFGSIS
                     FVTYAVSFWGPPYVLRTFYPDPTGPGLFIGGMTAAEEVASIIGWGSAISAAAGVIIGG
                     VVSDLWRRTDPRGRIFVNMASVILAAPLTWFIFTTDSVATFYWVFPLAHLAGAMWVGA
                     AVATLQDLVLPRMRATAGATYILGTTMIGLALGPYFAGKVADVAESLRAGIFALYLIV
                     PLTLLGLWLGSRRIAELEATKVERARAAGELV"
     gene            complement(2005..2565)
                     /locus_tag="VF547_06945"
     CDS             complement(2005..2565)
                     /locus_tag="VF547_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX8262593.1"
                     /translation="MRQAGLLFIGGAVLAIAACATDTDATQPEGAIVSTIGPPVLNAD
                     LRDAQGRTRARATAETAGDSLRVRIEATGMSPGTYGAHLHTVGRCDAPDFTSAGAHWN
                     PTGQMHGKDNPKGMHKGDLPNLMVGADGRGSFEYTIPNAGLSGMLPNRIIDADGASVV
                     IHARPDDYRTDPSGNSGGRIACGVLG"
     gene            complement(2619..3932)
                     /locus_tag="VF547_06950"
     CDS             complement(2619..3932)
                     /locus_tag="VF547_06950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX8262594.1"
                     /translation="MRIAMIGTGYVGLVSGACFSDFGHDVVCVDKDSRKIEALKRGVM
                     PIFEPGLDDLVQRNVRGGRLSFTTDLEEGIAGADAVFIAVGTPSRRGDGHADLSYVFG
                     AAEEIARALRGPVVVVTKSTVPVGTGDEVERIIRETAPEAKAWVVSNPEFLREGAAIE
                     DFKRPDRIVVGTEDEAARELMREIYRPLYLNKAPLLFTGRRSAELIKYAANAFLATKI
                     TFINEVSDLCEMVGADIQDVARGIGLDNRIGAKFLHAGPGYGGSCFPKDTLALLKTAE
                     DHQAPLRIVESVVKVNEARKRAMGRKVVQALGGEARGRTVALFGLAFKPNTDDMRDAP
                     SLSIVQALQDAGAKVRGYDPECIEQARPLMPGVDFFDSPYLAAAGADAVVLVTEWDVL
                     RALDLRRLAQDMAQPIFVDLRSVYPPEEVEEAGLRWFGIGRPPRG"
     gene            complement(4105..5232)
                     /locus_tag="VF547_06955"
     CDS             complement(4105..5232)
                     /locus_tag="VF547_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8262595.1"
                     /translation="MRKLAIAVALSSTLLATPAFARDGAWYVGGEFGAMIVEDTNVDI
                     GAVDNALRLNYDYGYDGGLFVGYDLGAFRIEAEAAYKKADLESFNTIIALPGENASTF
                     PPSREFAGGSASALSFMINGMLDFGDDDGISGFVGGGIGMARVKANNQRVFANTAPFL
                     DDSDSKLAWQVFAGVRQAISDNIDVTVKYRFFNVDKVRMVAFNGNESEHRFRSHSLLG
                     GLTFNFGGAEPVPEVVPPPPPPPYVPPVVEQPPAPPPPPPPCVAGPFMVFFDWDKDDI
                     TPQAAAILDNAASSYQTCGQAQVMIAGHADRSGSDQYNVGLSQRRAANVRSYLAGRGI
                     PDGVMTTEAFGESRPLVETADGVREPQNRRVEITYGPGSGW"
     gene            complement(5436..5933)
                     /locus_tag="VF547_06960"
     CDS             complement(5436..5933)
                     /locus_tag="VF547_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEX8262596.1"
                     /translation="MADSTPRFALPYILPGQAQKELFHNEALARIDLALHPAVEGGPE
                     ATPPTGPQEGQCWIVASPGSGDWAGRDGMLAMWTEGGWRFLAPAPGTVAWNKAASLPL
                     LWDGAQWREGALLCAGLVVNGVQVVGPRQAAVPSPSGGTIIDEEARVAINALTAALMS
                     HGLIE"
     gene            complement(5946..8123)
                     /locus_tag="VF547_06965"
     CDS             complement(5946..8123)
                     /locus_tag="VF547_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8262597.1"
                     /translation="MATLVLTAVGTAIGGPIGGAIGAVLGQQIDGRLFAPKPRQGPRL
                     GDLAVQTSSYGSQIPKLFGTMRVAGTVIWATDLKEERTRGGGGKGQPRTVSYSYSANF
                     AVALSARPILGVLRIWADGKLLRGAAGDFKTATGYRLHLGGEDQGVDPLIASAEGPGQ
                     APAYRGLAYAVFEDFQLADYGNRIPSLTFEVEADAAAPTVGAVAAELAREAVTAGQTA
                     ALTGFAAGGDSVRAALAALAELEPLSLIDDGERLVLTSGEAAAPLAIAEAELGAAAAG
                     AGGRSEWQRRRGDSVPAEATLAYYEPSRDYQTGLQRASRPGAGLGSDRSSVAAALEAA
                     TAKSLVTRRLERLWAERRSGKVHLGWARLGLSAGRRIRIAGRPGDWKLERWTLDRMML
                     TLELVGVPGAGSAAGQASAGRPVDQPDLIHGPTTLVLLDLPLHAEALPNRPRLLVAAA
                     GRQAGWRRAELSASFDGGASWAAAGPTAPAAVLGVALSAPGAGGAALIDERNQIEVEL
                     LNEAMWLESRSEAALAAGDNLAVLGDELIQFGIATPLGGRRFRLSRLLRGRRGTEWAA
                     GLHSAGEPFALIEAESLAPLEAPPGSLGGEVRLLATGLGDGEGTVAQRPITGEALRPP
                     APVHLRAERRPNGDLALGWVRRSRHGWVWPSGSDTPLGEETESYRLTLAGAGFERSVT
                     VASPAYLYTAAEQDADGMAGPLAVEVVQLGTAAPSRPATLVSE"
     gene            complement(8125..8532)
                     /locus_tag="VF547_06970"
     CDS             complement(8125..8532)
                     /locus_tag="VF547_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HEX8262598.1"
                     /translation="MSLRGHRIARRARSLVGVRFRPQGRDPMLGLDCVGTAAVAAGVP
                     PGRLPSDYALRGQHLAAIEHELCDLGCQPVPLERAVAGDVLVCEAGPAQFHIVIRAWS
                     SFIHADAGLRRVVERPLPVPWPISGAWRFFEGL"
     gene            complement(8534..9352)
                     /locus_tag="VF547_06975"
     CDS             complement(8534..9352)
                     /locus_tag="VF547_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEX8262599.1"
                     /translation="MTGFVQRALTTVAFCWRVERRDGVCLGFTSHDRDLEIGGLVYRA
                     APGMLPSAVSVSDGFDASTLDVAGALTSDSIGEADLAAGRWDRAAVALFLVDWEDPDG
                     ERVPLVRGELGDVATKANGFEAELRGPTGLLERAVVEQTSPECRAELGDRRCRADMAG
                     RVRMTRVTATPAENVVVVADASNSPGAYAFGRLRWLGGANSGLESAIAASDGPSLTLQ
                     EPPPFAIAEGDRVEISEGCDKSFATCAARFANAENFRGEPHLPGMDLLTRYPGA"
     gene            complement(9355..11640)
                     /locus_tag="VF547_06980"
     CDS             complement(9355..11640)
                     /locus_tag="VF547_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEX8262600.1"
                     /translation="MGHWLAPPGAAKQIGFVKRFDARLWTVNFPRPMMAAAVTTGPHS
                     LRVDAVFHKANDLAGLIWEAEDRFDHPLLAYETARDFRACRLAFRWRSGGIRALDEVD
                     GPTLTVEGRDPAGAARSWYVRLWNHAEGTPEDALVELDFAALAGGWSGDDPVWAGDVD
                     RMFVSMVAPGHSGADEPLAAPAEGWAELSEMRCDGSGSVLATGDTLVPEHGLRMATAY
                     DDLYHLTPARVLRNCLQLGYRGPINHYVGMSHYFRLEAHGGGFFVSLAGGVLNAACAA
                     WHRDFAERAGALGYEPIFSLSYELLDQHAWGDWKQRAEDGSPALTGWSPPSTLLSPAH
                     AGAMGYLRLVAQAFAAIAAAAGLRVRIQIGEPWWWVTADGRICLYDSAAVAAFAPVSI
                     ADVRAPLDPAQRSTLDSAGAVLADSTAALAAAVRSVAADAQILLLAYLPAVLDGPDVR
                     RANLPPGWASPAFDVLQLEDYEWTTAGNAGASARGIAAAQARLGYPADRQHYFAGFVL
                     APEDRAQWHRVAAAAAAARRRGAGEVFVWALPQVLRDGFTWFDLGDGDVDAFEDVRFP
                     VALGREASVEPAFSTAIVTGAGGAEQRNSDWSNARLRFDAGPGVRGEEDLHTLIAFFR
                     ARRGAAVGFRFEDPFDNSSNGMTGAPGASDQRLGSGDGVRTEFALVKHYDRQERRITR
                     PVAGTVRVSVGGVERAGGWTLAPLGLIRFDEAPASGAEVRAGFRFDVPVRFAEDRLTV
                     GRATFEAGEIPSAPLIEIREG"
     gene            complement(11698..12267)
                     /locus_tag="VF547_06985"
     CDS             complement(11698..12267)
                     /locus_tag="VF547_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HEX8262601.1"
                     /translation="MDEEIERLLVTVRADTGAFAKDVAEMKGQLEGPLAAGIERAGRV
                     LESALIRAVRTGKLGFEDLKKVALAAMAEIAAAAIRAGIDAAAGGGRGGGLLTAAAQL
                     LGLLAGAPGRATGGPVAPGRPYLVGERGPELFVPTASGRVEAARGAVREIRMSITVNA
                     AAGEAPAALARSGRQVARAVKQALMRVED"
     gene            complement(12347..12547)
                     /locus_tag="VF547_06990"
     CDS             complement(12347..12547)
                     /locus_tag="VF547_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEX8262602.1"
                     /translation="MSGGQRFGECAARLAGLAGALLGWRPDEFWRSTPAELAAVIEAM
                     LGPESEAASRSDLERLMERFPD"
BASE COUNT         1811 a         4759 c         4281 g         1857 t
ORIGIN      
        1 ggccttgccg tcggccggac gaagccgctc gaggtcgcga aggttggtgg agtcgagagc
       61 gacgatatgg tcgaagcgac ggaagtcgtc gctcgtcact tggcgcgcct tgaggtgcga
      121 gatgtccacc ccgttgcggg ccgccaccgc cgcggctcgc ggatcgggcg ggtagccgat
      181 gtgccagtcg cccgtgcccg cggagtcgat ctcgacctca aggccgagcc gctcgcaggc
      241 gcgccggcac gccgcctccg ccaggggcga gcggcagatg ttgcccagac agacgaacag
      301 gacggagctt ttcaccggcg atgccttgtt gccaaagcgc gccgctctgc tagccacatg
      361 cgaagaacag cacaatgaga ggtgtatggc ggcgaacccc gaagcgaccg cgcccgcggc
      421 ctccccgccc gtgcgggtcg gcgcctacgc ctggtacgcc ctcatcgtcc tggttctggt
      481 ctacgcgctg aacttcatcg accgccagat cctctcgatc ctcgctcagg acatcaagcg
      541 cgacctcggc ctcgaggatg cccagatcgg cttcctctac ggcaccgcct tcgcggtctt
      601 ctacgccctg ttcggaatac cgctcgggcg gctggccgac agctggtatc gcggccgcgt
      661 gatggcgatc gggctcgcgc tctggtcgtc gatgacggcc ctgtccggct tcgccagcag
      721 cttcacgatg ctcgccgccg cccgcatcgg cgtcggagtg ggcgaggccg ccgcctcgcc
      781 cgccgcctat tcgatgatct cggactggtt ccccaaggag aagcgcgcga ccgcgctctc
      841 catctattcg agcggtctct atatcggcgg cgcgctcagc ctgccgatcg gcggcttcgt
      901 tctgagccgc tggaacctcg cttttccggt tccgtccgaa gctccgctcg gcctggtcgg
      961 ctggcaggcg gccttcctgg ccgtcggcat accggggctg ctgatcgcgc tgtgggtgct
     1021 gtcgctgagg gagcctcagc gcggcgcctc cgacggcctg ccgcagccga tcgtccggcc
     1081 gcacgcgtgg cgcgatttcg gacgcgagct ggtcgcgatc ctgccgccgc tgaccctgat
     1141 caacgtctcg cgactccccg gcaacgcgat gaacctcaat ctgatcgtgc ttgggatcgt
     1201 cgccgtctgc gccttgggac tggtccggct gaccggggac gtgccgcaat ggtccgccta
     1261 cgcactcggc gtctacgcgg tcttctcctg ggtccagtcg ctcaagcatc gcgatccggc
     1321 gacccatcgg ctgatctggg ggacgccgat gacgatcgtc ctcgccatct cgttcggcag
     1381 catctcgttc gtcacctacg cggtcagctt ctggggcccg ccctacgtgc tgcgcacctt
     1441 ctacccggat ccgaccgggc cgggcctctt catcgggggc atgaccgcgg ccgaggaggt
     1501 cgcctccatc atcggctggg gctcggcgat ctccgcggcc gcgggagtga tcatcggcgg
     1561 ggtggtttcg gacctctggc gccggaccga cccgcgcggg cgaatcttcg tgaacatggc
     1621 ctctgtgatc ctggcggcgc cgctgacctg gttcatcttc acgacggatt cggtggcgac
     1681 cttctactgg gtcttccccc tcgcccacct ggccggcgcg atgtgggtcg gcgccgccgt
     1741 cgcgacgctc caggatctcg tgctgccgcg gatgcgcgcc acggccggcg cgacctacat
     1801 cttggggacg accatgatcg gcctcgcgct cggcccctat ttcgcgggca aggtcgccga
     1861 cgtcgccgag agcctgcgcg ccggcatctt cgcgctttac ctgatcgtcc cgctcaccct
     1921 cctcgggctg tggctgggca gccggcggat cgccgagctc gaggccacca aggtcgagcg
     1981 cgcccgcgcc gccggcgagc tggtctagcc caggaccccg caggcgatcc ggccgccgct
     2041 attgcccgac ggatcggtgc gatagtcgtc cggccgggca tggatcacca ccgaggcccc
     2101 gtccgcatcg atgatgcggt tgggaagcat gccggagagg cccgcgtttg ggatcgtata
     2161 ttcgaagctt ccccggccgt cggcgccgac catgaggttg ggaaggtcgc ccttgtgcat
     2221 ccccttggga ttgtccttgc cgtgcatctg accggtcggg ttccagtggg cgccggcgct
     2281 ggtgaagtcc ggcgcgtcgc agcggccgac cgtgtggaga tgggcgccgt aggtgcccgg
     2341 gctcatcccc gtcgcttcga tccgcacccg caggctgtcg cccgccgttt ccgcggtcgc
     2401 ccgggcacgg gtgcggccct gggcgtcgcg cagatcggcg ttgagaaccg gcggtccgat
     2461 cgtcgacacg atcgcgcctt cgggctgcgt tgcatcggtg tcggtcgcgc aggcggcgat
     2521 ggcgagcacc gcgcctccaa taaagagcaa gccggcctgt ctcatcccac gtctccttcg
     2581 gacccctgaa cgatccgatg ctgctagggt tgccaggctc agccgcgcgg cggacggccg
     2641 atgccgaacc agcgcagccc cgcctcctcc acctcctcgg gcggatagac gcttcgaagg
     2701 tcgacgaaga tcggttgcgc catatcctgg gccagacgcc tcaggtcaag cgcccgcagc
     2761 acatcccatt cggtcaccag gacgaccgcg tcggccccgg ccgccgccag ataggggctg
     2821 tcgaagaagt cgacgccggg catcaagggc cgcgcctgct cgatgcattc cggatcgtag
     2881 ccgcgaacct tggcgccggc atcctggagc gcctggacga tgctcagcga aggggcgtcg
     2941 cgcatgtcgt cggtgttggg cttgaaggcc aggccgaaca gcgccacggt gcggccccgc
     3001 gcctccccgc ccagggcctg gacgaccttg cggcccatgg cgcgcttgcg cgcttcgttc
     3061 accttgacca ccgactcgac gatgcgcagc ggcgcctggt gatcctcggc ggtcttgagg
     3121 agggcgagcg tgtccttcgg gaagcaggat ccgccatagc cgggaccggc atggaggaac
     3181 ttggcgccga tgcggttgtc gaggccgatg ccgcgggcga cgtcctggat gtcggcgccg
     3241 accatctcgc acaggtccga gacttcgttg atgaaggtga tcttggtcgc gaggaaggcg
     3301 ttggcggcat atttgatcag ctcggcgctg cggcggccgg tgaacagcag aggcgccttg
     3361 ttgaggtaga gcggccgata gatttcgcgc atcagctccc gggccgcctc gtcctcggtg
     3421 ccgacgacga tccggtcggg ccgcttgaaa tcctcgatcg cggcgccctc gcgcaggaat
     3481 tccggattgg agactaccca ggccttcgcc tcgggagcgg tttcccgaat gatccgctcg
     3541 acctcgtcgc cggtgccgac cggcaccgtc gacttggtca ccaccacgac cgggccgcgc
     3601 agcgctcggg cgatctcctc ggcggctccg aagacgtagc tgaggtcggc atggccgtcg
     3661 ccgcgtcgcg acggcgtccc gaccgcgatg aacaccgcat cggcgcccgc gatcccctct
     3721 tccaggtcgg tggtgaacga gagtcggccg ccgcggacgt tgcgctgcac gagatcgtcc
     3781 agccccggct cgaagatcgg catcacgccc cgcttgagcg cttcgatctt ccgtgaatcc
     3841 ttgtcgacgc agacgacgtc gtggccgaaa tctgaaaagc aggcgccgga caccaggccg
     3901 acatagcccg taccgatcat cgcgatgcgc atcgaaaatc cttggctggg tggagaggcg
     3961 cccgcttagc ccgcgccggt tcggcgcgac aagggctttc cgtgcccggg gaactccggc
     4021 gcgcgcgcca aaaagaaagg agccggcccg aaagccgact ccttccttgt tcttggcgct
     4081 ccgccgcgag cggcggaaac ccgcttacca gccggagccg ggtccgtagg tgatctccac
     4141 gcgccggttc tgcggctcgc ggacgccgtc ggcggtctcg accagcggcc ggctctcgcc
     4201 gaacgcttcg gtggtcatga cgccatcggg gatgccgcgg ccggccaggt acgaacggac
     4261 gttcgcggcg cgacgctgcg acagcccgac gttgtactgg tccgaacccg aacggtcggc
     4321 gtggcccgcg atcatcacct gggcctggcc gcaggtctga tacgaggaag ccgcgttgtc
     4381 gaggatcgca gcggcctgcg gcgtgatgtc gtccttgtcc cagtcgaaga agaccatgaa
     4441 cggaccggcg acgcacggcg gcggcggcgg cggcggcgcc ggcggctgct cgaccacggg
     4501 cgggacgtag ggcgggggcg gcggcggcgg cacgacttcc ggcaccggct cggctccgcc
     4561 gaagttgaag gtcaggccac ccaacaggct gtgcgagcgg aagcgatgct cggactcgtt
     4621 gccgttgaac gcgaccatgc gcaccttgtc gacgttgaag aagcggtact tcacggtcac
     4681 gtcgatattg tcgctgatcg cctgacggac gcccgcgaag acctgccagg caagcttcga
     4741 atcgctgtcg tcgaggaagg gagcggtgtt ggcgaagacg cgctggttgt tcgccttcac
     4801 ccgcgccatg ccgataccgc cgccgacgaa gcccgaaatg ccgtcgtcgt cgccgaagtc
     4861 gagcatgccg ttgatcatga agctcagagc gctggcgctg ccgcccgcga actcgcgcga
     4921 gggcgggaag gtggacgcgt tttcgccggg aagggcgata atcgtgttga aggactcgag
     4981 gtccgccttc ttgtaggcgg cttcggcttc aatccggaac gcgccgaggt cataaccaac
     5041 gaacaggcca ccatcgtagc cgtaatcgta gttgagcctc aaggcgttat cgacggcgcc
     5101 gatatcgacg ttcgtgtctt cgacgatcat cgcaccaaat tcgccgccaa cgtaccaggc
     5161 gccatcgcgc gcaaaggctg gtgttgcgag caaggtggat gaaagcgcca ctgcgatggc
     5221 gagcttccgc ataaactttc ccctttcttc tgcgtcccta cgggaacgac catgaactca
     5281 atacggtaag cggagtttcc gcgcaagcac gcttatcccc acaaatgttg ccgaaatgcc
     5341 tcagttaatc cgttaccgaa tcgttcgaga cccctcgtca gacggcccgc aaacgaccgt
     5401 ctgccccctt ggtttcatgt cggcaacaaa aatgctcact cgatcagccc gtgtgacatt
     5461 aatgccgcag tcagggcatt gatcgccacc cttgcctcct cgtcgattat cgttccgcca
     5521 gaaggacttg gcaccgccgc ctggcgcggc ccgacgacct ggacgccgtt gacgaccagc
     5581 cccgcgcaga gcagcgcgcc ttcgcgccac tgggccccat cccagagcag tggaagggag
     5641 gccgccttgt tccaggccac cgtgcccggc gcgggcgcga ggaagcgcca tccgccttcc
     5701 gtccacatcg ccagcatgcc gtcgcgcccc gcccagtccc cgcttcccgg cgaggccacg
     5761 atccagcatt ggccctcctg cggtccggtc ggcggcgtcg cctccggccc gccctcgacg
     5821 gccggatgga gcgccagatc gatccgcgcc agcgcctcgt tgtggaagag ctccttctgc
     5881 gcctggccgg ggaggatgta ggggagcgcg aagcgcggtg tcgaatccgc cacgatatct
     5941 tctcctcatt ccgaaacgag cgttgcgggc cgcgacggcg ccgccgttcc gagctggacg
     6001 acctcgacgg cgaggggccc cgccatcccg tccgcgtcct gctccgcagc cgtgtagaga
     6061 taggctgggg aagccacggt caccgaccgc tcgaaccccg caccggcgag cgtcagccgg
     6121 tagctctccg tctcctcgcc gagcggcgtg tcgctgccgc tcggccacac ccagccgtgg
     6181 cggcttcgcc tgacccagcc gagcgcgagg tcgccgttcg gccgccgctc cgcgcgcagg
     6241 tgcacgggcg caggcggccg cagcgcttcg ccggtgatcg gcctctgcgc gaccgtgccc
     6301 tcgccgtcgc ccaggccggt cgccagcagg cgcacttccc cgcccagcga tcccggcggc
     6361 gcctcgagcg gcgcgaggct ctccgcctcg atcagcgcga acggctcccc ggccgagtgg
     6421 agcccagccg cccactccgt ccccctccgg cccctgagca gccgggagag acggaagcgc
     6481 cggccgccga gcggagtcgc gatcccgaac tggattagct cgtcgccgag caccgcgagg
     6541 ttgtcgccgg cggcgagcgc ggcctcgctg cggctctcca gccacatcgc ctcgttgagc
     6601 agctcgacct cgatttggtt gcgctcgtcg atcagcgccg cgcccccggc gcccggcgcg
     6661 ctcagggcga cgccgaggac cgccgccggc gccgtcggtc cggcggcggc ccagctcgca
     6721 ccgccgtcga agctcgcgct gagctcggcg cggcgccagc ccgcctgccg gccggccgct
     6781 gcgaccagca gacgcggccg gttcggcagc gcctcggcat gaagcggaag gtcgagcagg
     6841 acgagggtcg tggggccatg gatgaggtcg ggctggtcga ccggacggcc cgcgctcgcc
     6901 tgtcccgctg ccgagcccgc gcccggcacg ccgacgagct cgagggtcag catcatccgg
     6961 tcgagcgtcc agcgctcgag cttccagtcg cccggccgtc cggcgatacg gatccggcgc
     7021 ccggcggaaa gcccgagccg cgcccagccg agatggacct ttcccgaccg ccgctcggcc
     7081 cagagccgct cgagccgccg ggtcacgagc gacttcgccg tcgccgcctc gagcgccgcg
     7141 gcgacggagc tgcggtcgct gccgagcccg gcccccggcc ggctcgcccg ctgcagtccg
     7201 gtctgatagt cccggctggg ctcgtaatag gcgagcgtgg cctccgcggg gacggaatcg
     7261 ccgcggcgcc gctgccattc gctgcgtccg cccgcgccgg ccgccgccgc gcccagctcg
     7321 gcttcggcga tggccagcgg agcggcggcc tctccgctcg tcaggacgag cctctcgccg
     7381 tcgtcgatca gcgagagcgg ctccagttcg gcgagcgcgg cgagcgccgc ccgaacgctg
     7441 tcgccgccgg cggcgaagcc ggtcagagcg gcggtctgcc cggccgtcac cgcctccctt
     7501 gccagctcgg cggccaccgc cccgacggta ggggcggcgg catcggcctc gacctcgaag
     7561 gtcagcgacg gaatgcgatt gccgtagtcg gcgagctgga aatcctcgaa cacggcatag
     7621 gcgagcccgc gataggcggg cgcctggccc ggcccctcgg ccgaggcgat cagcgggtcg
     7681 accccctggt cctcgccgcc gaggtggaga cggtagccgg tcgccgtctt gaaatccccg
     7741 gcggcgccgc gcagcagctt gccgtccgcc cagatccgca ggacgccgag gatcggccga
     7801 gccgacaggg cgactgcgaa gttcgcggaa tagctgtagc tgaccgtcct gggctggccc
     7861 ttgccgccgc cgccccgggt ccgctcctcc ttgaggtcgg tcgcccagat caccgtgccg
     7921 gcgacgcgca tcgtcccgaa cagcttgggg atctggctgc cgtagctcga cgtctgaacc
     7981 gccagatcgc cgaggcgcgg cccctggcgc ggcttgggcg cgaacaggcg cccgtcgatc
     8041 tgctggccga ggaccgctcc gatggcgccg ccgatcgggc ctccgatcgc ggtgccgacg
     8101 gcggtgagga caagggtggc catctcacaa tccctcgaag aaccgccagg cgcccgagat
     8161 cggccagggc accggcagcg gccgctcgac cactcggcgc aggcctgcat cggcatggat
     8221 gaagctggac caggcacgaa tgacgatgtg gaactgggcc ggtcccgcct cgcagacgag
     8281 gacgtcgccc gcgaccgctc gctccagcgg caccggctgg cagcccaggt cgcacaattc
     8341 gtgctcgatc gccgccaggt gctggccgcg cagcgcatag tcgctcggca gccggccggg
     8401 cggcactccc gcggctaccg ccgcggttcc gacgcagtcc agccccagca tggggtcgcg
     8461 gccctgcggc cggaagcgga cgccgaccag cgagcgcgcc cgccgcgcga tccgatgtcc
     8521 tctcaagctc atgtcaggcc cccggatagc gagtcagcag gtccattccc ggcagatgcg
     8581 gctcgccgcg gaaattctcg gcgttggcga agcgggccgc gcaggtggcg aagctcttgt
     8641 cgcagccttc gctgatctcg acccggtcgc cctcggcaat ggcgaagggg ggaggctcct
     8701 gcagcgtcag cgacgggccg tccgacgccg cgatcgcgct ctccagcccg ctattggcgc
     8761 cgccgagcca gcgcagcctg ccgaaggcat aggcgccggg gctgttcgag gcgtcggcga
     8821 cgacgacgac attttcggcc ggagtcgcgg tcacccgggt catcctcacg cgccccgcca
     8881 tgtcggcccg gcaccggcgg tcgcccagct cggctcggca ttcgggcgac gtctgctcga
     8941 ccaccgcccg ctccagcagg ccggtcggcc cgcgcagctc cgcctcgaag ccgttcgcct
     9001 tggtcgcgac gtcgccgagc tcgcctcgaa cgagcggcac tcgctccccg tccggatcct
     9061 cccagtcgac caggaacagc gcgaccgcgg cccggtccca gcgtccggcg gcgaggtccg
     9121 cctcgccgat ggagtcgctc gtcagcgccc cggccacgtc gagggtcgag gcgtcgaagc
     9181 cgtccgatac cgagacggcc gacggcagca tgcccggcgc ggcgcggtag acgaggccgc
     9241 cgatctcgag gtcgcgatcg tggctggtga agccgaggca gaccccgtcc cggcgctcga
     9301 cccgccagca gaaggcgacc gtggtcagcg cgcgttgcac gaagccggtc atgatcagcc
     9361 ctcccggatc tcgatcagcg gcgccgaagg gatttcgccc gcctcgaacg tcgcccggcc
     9421 gacggtcagc cggtcctcgg cgaagcggac cggcacgtcg aagcggaagc ccgccctcac
     9481 ttcggccccg ctcgccggcg cctcgtcgaa gcggatcagg ccgagcggcg ccagcgtcca
     9541 tcctccggcc cgctcgacgc cgccgaccga cacccggacc gtgcccgcga ccggccgagt
     9601 gatccgccgc tcctgccggt cgtaatgctt gaccagcgcg aactcggtgc ggacgccgtc
     9661 gccggagccg agccgctggt ccgacgcccc tggagcgccg gtcatgccgt tcgagctgtt
     9721 gtcgaacgga tcctcgaagc ggaagccgac cgccgcgccg cgacgggccc ggaagaaggc
     9781 gatcagcgtg tggaggtcct cctcgccgcg cacgccgggg ccggcgtcga agcgcaggcg
     9841 ggcgttcgac cagtcgctgt tgcgctgctc ggcgccgccc gccccggtga cgatggcggt
     9901 cgagaaagcc ggctcgacgc tcgcctcgcg tccaagcgcc accgggaagc gcacgtcctc
     9961 gaatgcgtcc acgtcaccgt ctcccaaatc gaaccaggtg aagccgtcgc ggagcacctg
    10021 cggcagcgcc cagacgaaga cctcgcccgc gccccgccgc cgcgccgcgg ccgccgccgc
    10081 cgccacccgg tgccattgcg cccggtcctc gggcgcgagc acgaagccgg cgaaataatg
    10141 ctggcggtcc gccgggtagc cgagccgggc ctgcgccgcg gcgattccgc gcgcgctcgc
    10201 cccggcattg ccggcggtcg tccattcgta atcttcgagc tgcagcacgt cgaaagccgg
    10261 gctcgcccag ccgggcggca ggttcgcgcg cctgacgtcc ggcccgtcga gcacggccgg
    10321 caggtacgcg agcagcaaga tctgcgcgtc cgctgcgacg ctccggacgg ccgccgccag
    10381 cgctgcggtt gaatcggcca gcaccgcccc ggcggagtcg agcgtggacc tctgcgccgg
    10441 gtcgagcggg gcccgcacgt cggcgatgga aaccggcgcg aacgccgcca ccgccgccga
    10501 gtcgtagagg cagatgcgcc cgtccgccgt cacccaccac cagggctcgc cgatctggat
    10561 ccgcacccgc agccccgcgg ccgccgcgat cgcggcgaaa gcctgcgcca ccagccgaag
    10621 atagcccatc gcgccggcat gggcgggcga aagcagcgtc gacggcggcg accagccggt
    10681 cagcgccggc gatccgtcct ccgcgcgctg cttccagtcg ccccaggcat gctggtcgag
    10741 caattcgtag gagagcgaga agatcggctc gtagcccagc gccccggccc gctccgcgaa
    10801 gtcgcgatgc caggccgcgc aggccgcgtt cagcactccg ccggcgaggc tgacgaagaa
    10861 gccgccgcca tgggcctcga gccggaaata atggctcatc ccgacataat ggttgatcgg
    10921 cccgcgatag ccgagctgca ggcagttccg cagcacccgg gcgggcgtca ggtggtacag
    10981 atcgtcatag gccgtcgcca tccgcagccc atgctccggg accagcgtgt cccccgtcgc
    11041 gagcaccgat cccgagccgt cgcagcgcat ttcgctgagc tccgcccagc cctcggccgg
    11101 agcggccagc ggctcgtccg cgccgctgtg gccgggcgcc accatcgaca cgaacatccg
    11161 gtcgacgtcg ccggcccaga ccgggtcgtc gcccgaccat ccccccgcga gcgccgcgaa
    11221 gtcgagctcg accagcgcat cctccggcgt cccctccgca tggttccaca gccgaacgta
    11281 ccagctgcgc gccgcccccg ccgggtcccg cccttcgacg gtcagggtcg ggccgtcgac
    11341 ctcgtcgagc gccctgatcc ccccgcttcg ccagcggaag gcgagccggc aggcgcggaa
    11401 gtcccgggcc gtctcatagg cgagcagcgg atggtcgaac cggtcctccg cctcccagat
    11461 cagcccggcc aggtcgttcg ccttgtggaa gacggcgtcg acccgaagcg aatgcggtcc
    11521 ggtggtcacc gccgccgcca tcatcggccg cgggaagttg acggtccaca agcgcgcgtc
    11581 gaaacgcttg acgaacccga tctgcttcgc cgcccccggc ggcgccagcc aatggcccat
    11641 ttcgaacctc cctcgccgcc gcgaagcgcg gcgccgccca gacccggcgc gatccgctca
    11701 gtcctcgacc cgcatcagcg cctgcttcac cgcgcgcgcc acctgccggc cggagcgggc
    11761 cagcgccgcc ggggcctcgc ccgccgccgc gttgacggtg atgctcatcc ggatctcgcg
    11821 gacggcgccc cgagccgcct cgacccgccc gctcgccgtc ggcacgaaca gctccggccc
    11881 gcgctcgccg accagatagg gccgccccgg cgccaccggc ccgccggtcg cccggcccgg
    11941 cgctccggcc agcaggccca gcagctgcgc cgccgccgtc agcaggccgc cgcccctgcc
    12001 gccgcccgcg gccgcgtcga ttccggctcg gatagccgcg gccgcgatct cggccatcgc
    12061 cgccagcgcc accttcttga ggtcctcgaa gccgagcttg ccggtcctca ccgcccggat
    12121 cagggcgctc tccagcaccc ggccggcccg ctcgatcccc gcggcgagcg gcccttcgag
    12181 ctgccccttc atctccgcca cgtccttcgc gaacgcgccc gtatcggcac gcacggtgac
    12241 cagcagccgc tcgatctctt cgtccatcgg gaaaatcctc gccattgccg gatgggggcg
    12301 accgggcgcg gcctggcgga tcgtttcccg ccccgatctg caagtctcag tccggaaacc
    12361 gctccatcag ccgctcgagg tcgctgcgcg acgccgcctc gctctccggc ccgagcatcg
    12421 cctcgatcac ggccgccagc tccgccgggg tcgaccgcca gaactcgtcg ggccgccagc
    12481 cgagcagcgc gcccgccagg ccggcgagcc gcgccgcgca ctcgccgaac cgctgcccac
    12541 cgctcatcgc gagcccgtcg aggcgccctg caatatctgt ccgagcagga ccttcagcac
    12601 cggcgtcgcc ccggccaggc ctcgctcgca gatcgcctcg ccgatgcgct ggcgagtcag
    12661 ggcttccggc cgcggatcgg cgcaatgcca gaacagggcc gccatctc
//
LOCUS       DASVQZ010000257         8240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_51913, whole genome shotgun sequence.
ACCESSION   DASVQZ010000257 DASVQZ010000000
VERSION     DASVQZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51913"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VF547_06995"
     CDS             <1..141
                     /locus_tag="VF547_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8262603.1"
                     /translation="TSEHRKAELPRWLHQYNWHRPHGSLQTQTPISRLGLDENNLLRL
                     HI"
     gene            complement(138..872)
                     /locus_tag="VF547_07000"
     CDS             complement(138..872)
                     /locus_tag="VF547_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8262604.1"
                     /translation="MSDPSTTEAITLAAIGDLHVSETCHGRYRTLFEEISATADVLAL
                     AGDLTNFGKVSEAEILAEDLRACTIPVVAVLGNHDYEAGAPDEVVRILQQAGVIMLGE
                     QATIIDDVGFAGAKGFIGGFGRGELGAFGEDAIKAFVDESRNEARLLENALRSLRTDR
                     VVAVLHYAPIPETVEGEPLEIYPFLGSSRLAHAIDRFDNVKAVVHGHAHRGAYSGRTP
                     RGVPVFNCAQFVVEPAFGRPYALLEI"
     gene            complement(850..1644)
                     /locus_tag="VF547_07005"
     CDS             complement(850..1644)
                     /locus_tag="VF547_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262605.1"
                     /translation="MSKQLEAFDPSLFEPPEEAVGFYVESLRLLQESGIPFLLSGTYA
                     LSCFTGITRPTKDLDVFCKPSDAPKILAFFQGRGFGIEMEDERWIGKVWRGDHFFDVI
                     FNISSASIPITDDWFREIYEAEVYGTTVRVTPPTQFILSKLFLQTRYRYDGADIVHTI
                     LIKHDEIDWHWLLSNMELYWEVLLINVLNFRFVYPTERDLIPRWLYEELLERLRAQDQ
                     MPPASIKICRGRLISPTDYVVDVSEWGFADVVGKGVDEKHERPQHH"
     gene            complement(1680..2123)
                     /locus_tag="VF547_07010"
     CDS             complement(1680..2123)
                     /locus_tag="VF547_07010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8262606.1"
                     /translation="MLIPLLLALAAPAQPAAVAPPPVVATPSGLRFQTLKAATGPRPG
                     AEDAVLIAYEGRLADGTVFDKSEEPVGMMVSGVIPGFQEALLLMNQGGTYRFWIPAAL
                     AYGAEGAGGGVIPPNAELDFTVTLVDIGRPAPAAAGPAAEPAPAD"
     gene            complement(2163..2237)
                     /locus_tag="VF547_07015"
     tRNA            complement(2163..2237)
                     /locus_tag="VF547_07015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2203..2205),aa:Gly,seq:gcc)
     gene            2480..3376
                     /locus_tag="VF547_07020"
     CDS             2480..3376
                     /locus_tag="VF547_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8262607.1"
                     /translation="MSAAEKEALEDFRRDVVEPSMDALVIVDFWAPWCGPCKQLTPIL
                     EKVAADYASKGVKLAKINVDENKIIAAQFRVQSIPTVYAVFQGQLVADLTPARTEGQL
                     TRFLDQILEQLPVAGEAQQLEAEIEPLIAMAEDVLAGGDAERAENIFRQILDMAPEHP
                     GVIAGLARALIAAERADEARALLDGVPDSAANDPAIARARSALALAEVARPGADTAGF
                     ERRIAADPDDHEARLELAGALMAAGDRDGAADQLLESIRRDREWNDGAARQRLLQLME
                     VVGLEDPWARDQRRRLSAILFT"
     gene            3376..4011
                     /locus_tag="VF547_07025"
     CDS             3376..4011
                     /locus_tag="VF547_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8262608.1"
                     /translation="MVDGSILRLPVFPLAGALLFPRAQLPLHIFEPRYRAMVRDALAT
                     NQLIGMIQPRASGEPPPLFDIGCIGRIAAAEELDDGRFNIVLEGLRRFRVAREAEVDT
                     LYRQVDAERTGFDDSAEPEPLGLAQRAELEREARRYADVLGYSVDWDAVTRLDDEMLV
                     NAIAQVAPLDVGSKQALLEAPDMAERCDLLVQFMQFQRMVPGGAEGPETLQ"
     gene            4008..4175
                     /locus_tag="VF547_07030"
     CDS             4008..4175
                     /locus_tag="VF547_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX8262609.1"
                     /translation="MTEDLLALLVCPATRTPLRWDAERQELVSDAAGLAYPVRDGVPV
                     MLVEEARRIAP"
     gene            4172..4582
                     /locus_tag="VF547_07035"
     CDS             4172..4582
                     /locus_tag="VF547_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262610.1"
                     /translation="MTAGTRLGLSGRWTGIYNYPAEHPPNRFEASLREVHGALAGTVW
                     ERDAASPGGGTLHALSEGRRDGPSVAFTKLYDDLERIADPIFYNGTVQPDGNEISGTW
                     SIVGVWSGTFLMIRSAGAEEAVGETVGETVGSGR"
     gene            complement(4579..5787)
                     /locus_tag="VF547_07040"
     CDS             complement(4579..5787)
                     /locus_tag="VF547_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8262611.1"
                     /translation="MDRADVIILGGGLIGLTLAVALDRHGLSAIVIDPADPERNVAPA
                     FDGRATAIASASWRMLEAIGIAGRLEDEGCPILSIRVSDGLEPGGIVFDAPEADDPLG
                     IMVENRRLRQVLREAALEASRVKLLMPAQAAETVRDSAGVRIALDDGRAAVAPLLVGA
                     EGRSSPTREAAAIPLARWRYDHSAVVLTVEHERHHEHAAYEIFYPSGPFAILPMQPGT
                     RSAIVWSVPAADAAAVVDLPDRAMAHEIEKRMGGFLGAVRVAGPRWTYPLGFHHAATM
                     TSERLALAGDAAHGIHPIAGQGLNLGFRDVAALTQVLVEGARLGMDLGDAQLLSRYER
                     WRSLDTLTVAAATDSLTRLYAIPGRTASAIRRFGMGVVQRVGPMKSQFMAEARGESGE
                     LPLLLRGLTV"
     gene            5978..8227
                     /locus_tag="VF547_07045"
     CDS             5978..8227
                     /locus_tag="VF547_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX8262612.1"
                     /translation="MRRSWALLIGGGLGALAFLLLLALASYSPTDPSLNTAAAGPVRN
                     WVGAPGAWSADLLLSLFGPPAALLLPLILLIGIRIAAGGEGTRWPRSLALTVLGLVLV
                     GGAASLLAGDAVNGLPASWGGAAGLSFAKLVELGLAAIGEPGIVEPFRIAAVGLFAAL
                     GLACCWFGLGLRADERAWIAARRLPRLARASADDGEELPEPERRPARTPVVAPAEPPR
                     TVINDRVRTPPQAKRPLRERQASLALGDSYQLPGLELLAPAPPPIGGALDKASLERNA
                     RLLESVLDDFSVKGEIVEVRPGPVVTMYELEPASGIKASRVIQLADDIARNMSALSAR
                     VATVPGRSVIGIELPNPKREMVVLSELIASHAFEDQVAQLPIVLGKNIAGDPVVADLA
                     PMPHLLVAGTTGSGKSVGLNCMILSLLYRLTPEQCKMILIDPKMLELSTYDDIPHLLA
                     PVVTEPAKAIRALKWTVEQMEERYRMMASIGVRQLSSFNAKVREAKAKGQTLGRRVQT
                     GYDPETGQPKYEMEELEYEVLPQIVVVVDELADLMMTAGKEVEFLIQRLAQKARAAGI
                     HLIMATQRPSVDVITGVIKANLPTRISFAVTSKIDSRTILGEQGAEQLLGKGDMLYMP
                     GGKQIARVHGPFVSDEEVRAVAEHWKAQGRPEYVQAVTEEPEDGGYMFEGQPTGEDDP
                     EAQLYRKAVQTVAESQKASTSYLQRQLRVGYNNAARLIERMEKDGIVSAPDHVGRREV
                     LVDPEGRPL"
BASE COUNT         1290 a         2850 c         2906 g         1194 t
ORIGIN      
        1 acctcggaac accgcaaggc cgagctgccc agatggctcc accaatataa ctggcaccgg
       61 ccacacggca gcctacaaac ccaaacaccc atcagccgac tcggcctgga cgagaacaac
      121 ctgttgaggc tccacatcta gatctccagc agggcgtagg ggcgtccgaa ggccggctcg
      181 acgacgaact gggcgcagtt gaagacgggg acgccgcgcg gcgtccggcc cgaataggcg
      241 ccgcgatggg catggccgtg gacgaccgcc ttgacgttgt cgaaccggtc gatggcatgg
      301 gcgaggcgcg acgagccgag gaagggatag atttccagcg gctcgccctc gaccgtctcc
      361 gggatcggcg cgtaatggag caccgcgacc acccggtcgg tccgcagcga gcgcagggcg
      421 ttctcgagca gcctggcctc gttgcgcgat tcgtcgacga acgccttgat cgcatcctcg
      481 ccgaaggcgc cgagctcgcc gcggccgaag ccgccgatga agcccttggc gcccgcgaaa
      541 ccgacgtcgt cgatgatcgt cgcctgctcg ccgagcatga tcactcccgc ctgctggagg
      601 atcctgacga cctcgtcggg cgctccggcc tcgtaatcgt ggttgccgag gaccgcgacg
      661 accgggatcg tgcaggcgcg caggtcctcg gcgaggatct cggcctcgct gaccttgccg
      721 aaattggtga ggtcgccggc cagggcgagc acgtcggcgg tcgccgagat ctcctcgaac
      781 agggtccggt agcggccgtg gcaggtttcg ctgacgtgga ggtcgccgat cgcggcgagc
      841 gtgatggcct cagtggtgct ggggtcgctc atgcttctcg tccactccct tgccgacgac
      901 gtcggcgaat ccccattcgc tgacgtcgac gacatagtcg gtcgggctga tcagccggcc
      961 ccggcagatc ttgatgctgg ccggcggcat ctggtcctgc gccctgaggc gctcgagcag
     1021 ctcctcgtac agccagcgcg ggatgaggtc ccgctcggtc gggtagacga agcggaagtt
     1081 gagcacgttg atcagcagca cctcccaata gagctccatg ttcgagagca gccagtgcca
     1141 gtcgatctcg tcatgcttga tgaggatggt gtggacgatg tcggcgccgt cgtagcggta
     1201 gcgggtctgc aggaagagct tggacaggat gaactgggtg ggaggggtga cccgaaccgt
     1261 cgtcccgtag acctccgcct catagatctc gcggaaccag tcgtcggtga tcgggatcga
     1321 ggccgaggag atgttgaaga tgacgtcgaa gaagtggtcg ccgcgccaga ccttcccgat
     1381 ccagcgctcg tcctccatct cgatcccgaa gccgcgcccc tggaagaagg cgaggatctt
     1441 gggcgcgtcg ctcggcttgc agaagacgtc gagatccttg gtcgggcggg tgattccggt
     1501 gaagcaggaa agcgcgtagg tccccgagag caggaagggg atgcccgatt cctgaagcag
     1561 cctcaggctc tcgacataga agccgaccgc ctcttccggc ggctcgaaca gggacggatc
     1621 gaaggcttcg agttgcttgc tcaccgcccg agaacggggc ccggagcaat gcgttccgct
     1681 cagtccgccg gggccggctc cgcggcgggc ccggcggcgg cgggagccgg gcggccgatg
     1741 tcgacgaggg tgacggtgaa atcgagctcg gcgttgggcg ggatgactcc gccgccggcg
     1801 ccctcggcgc cgtaggcgag cgcggcgggg atccagaagc ggtaggtgcc gccctggttc
     1861 atcagcagca gcgcctcctg gaagccggga atgacgcccg acaccatcat tccgaccggc
     1921 tcctcgctct tgtcgaagac ggtgccgtcg gcgagccgcc cctcgtaggc gatgagaacc
     1981 gcgtcctcgg ctcccggacg aggcccggtg gccgccttca aggtctggaa tctcagcccc
     2041 gatggagtcg ccacgacggg cggtggtgcg acggcggccg gttgcgccgg agccgcgagg
     2101 gcgagaagca gggggatgag catcgatact cccggatcgc ctgtctggcc tggccgaaaa
     2161 actggagcgg gcgaagggat tcgaaccctc gaccccaacc ttggcaaggt tgtgctctac
     2221 ccctgagcta cgcccgctct gagccgagcc ggcggagccg gctgaggtaa ggcggcggct
     2281 gctagcatgg ggcccgccac ccggcaagtg cctagtgcgc tccggggaag gcggggtccg
     2341 gcggcatgcg caaagggact ggatcccggc ctcgccggga aacgatcgcg gccagggagg
     2401 cttggcagaa gccctctcaa tcccacatag gcaatgaaga cgatccatgt ggaaaggcgg
     2461 aggcgtggcg acattgggga tgagcgcggc ggagaaggaa gcgctggagg acttccggcg
     2521 cgacgtcgtc gagccgtcga tggacgcgct ggtgatcgtc gatttctggg cgccctggtg
     2581 cgggccgtgc aagcagctga cgccgatcct cgagaaggtc gccgccgatt atgcgtcgaa
     2641 gggcgtgaag ctcgccaaga tcaatgtcga cgagaacaag atcatcgccg cgcagttcag
     2701 ggtgcaatcc atcccgaccg tctacgccgt cttccagggc cagctcgtcg ccgacctcac
     2761 cccggcccgg accgaaggcc agctgacccg cttcctcgac cagatccttg agcagctgcc
     2821 ggtggctggc gaggcgcagc agctcgaggc cgagatcgag cctctgatcg caatggcgga
     2881 ggacgtgctc gccggcggcg acgccgagcg cgccgagaac atcttccgcc agatactgga
     2941 catggcgccc gagcatcccg gagtgatcgc cggccttgcc cgggcgctga tcgccgcgga
     3001 gcgggccgac gaggcgcggg cgctgctcga cggagtgccg gacagcgccg ccaacgaccc
     3061 ggccatcgcc cgcgcccgct cggccctggc gctggcggag gtcgccaggc ccggagcgga
     3121 cacggccggg ttcgagcggc ggatcgccgc ggaccccgac gaccacgaag cccgactcga
     3181 gctggccggc gcgctgatgg ccgcgggcga ccgcgacggc gccgccgacc agcttctcga
     3241 gagcatccgc cgcgatcgcg aatggaacga cggcgcggca cggcagcgcc tgctccagct
     3301 gatggaggtg gtggggctcg aggacccttg ggcgagagac cagcggcggc ggctgtcggc
     3361 gatcctcttc acctgatggt cgacggctcg atcctgcgcc tacccgtctt cccgctggcc
     3421 ggggccctgc tcttcccccg cgcccagctg ccgctgcaca tcttcgagcc ccgctatcgg
     3481 gcgatggtcc gcgacgcgct cgccaccaat cagctgatcg ggatgatcca gccccgggcg
     3541 tcgggcgagc cgccgccgct gttcgacatc ggctgcatcg gccgcatcgc cgcggcggag
     3601 gagctggacg acggccgctt caacatcgtc ctcgaaggcc tccgccgatt cagggtcgcg
     3661 cgcgaggccg aggtcgacac gctgtatcgc caggtcgatg ccgaacggac cggcttcgac
     3721 gattcggcgg agcccgagcc gctcgggctc gcccagcgcg ccgagctcga gcgcgaggcc
     3781 cgccgctacg ccgacgtgct cggctattcg gtcgactggg acgccgtcac ccgcctcgac
     3841 gacgagatgc tggtcaacgc catcgcccag gtggcgccgc tcgacgtcgg ctcgaagcag
     3901 gccctgctcg aggcgccgga catggccgag cgctgcgacc tgctcgtcca gttcatgcag
     3961 ttccagcgca tggtgccggg cggcgcggaa gggccggaga cgctgcagtg acggaggatc
     4021 tgctggcgct gctggtctgt ccggcgaccc ggaccccgct cagatgggac gcggagcggc
     4081 aggagctggt gtcggacgcc gccggcctcg cttatccggt ccgggacggg gtcccggtca
     4141 tgctggtgga ggaggctcgg aggatcgcgc cgtgaccgcg gggacgcgcc tcggcctgag
     4201 cggccgctgg accgggatct acaattaccc ggccgagcat ccgccgaacc gattcgaagc
     4261 ctcgctgagg gaggtgcacg gcgcgctcgc cggcacggtg tgggagcgcg acgccgcctc
     4321 gccgggcggc gggacccttc acgccctgag cgagggccga cgcgacggac cgtcggtcgc
     4381 cttcaccaag ctctacgacg atctcgagcg catcgccgat ccgatcttct acaacggcac
     4441 ggtgcagccg gacgggaacg agatttccgg cacctggtcg atcgtcggcg tctggtcggg
     4501 gaccttcctg atgatccgaa gcgccggtgc ggaggaggcg gtcggcgaga ccgtcggcga
     4561 gaccgtcggg tccggccgct agacggtgag gcccctgagg aggagcggca gctcccctga
     4621 ttccccgcgc gcttccgcca tgaactggct cttcataggc ccgacccgct ggaccacgcc
     4681 cattccgaac cgccggatcg ccgaagccgt ccggcccgga attgcgtaga gccgggtgag
     4741 cgagtcggtg gcggcggcga cggtcagcgt gtcgaggctg cgccagcgct cgtagcgcga
     4801 caggagctgc gcgtcgccga ggtccatgcc gagccgtgcc ccttcgacga gcacctgggt
     4861 cagcgccgcg acgtcgcgga agcccaggtt cagcccctgg ccggcgatcg ggtggatgcc
     4921 gtgggcggcg tcgcccgcca gcgccagccg ctcagaggtc atcgtcgccg catggtggaa
     4981 gccgagcgga taggtccacc tcggtccggc cacgcgcacc gctccgagga agccgcccat
     5041 cctcttttcg atctcgtggg ccatcgcccg gtccggcagg tcgaccaccg cggcggcgtc
     5101 ggcggccggc accgaccaga cgatcgccga gcgggtgccc ggctgcatcg gcaggatcgc
     5161 gaaggggccc gacggataga agatctcgta ggccgcatgc tcgtgatggc gttcgtgctc
     5221 gacggtcagg accaccgccg aatggtcgta gcgccagcgc gccagcggta tggccgcggc
     5281 ctcgcgcgtg ggcgagctgc ggccctcggc gccgaccagc agcggcgcga cggcggcccg
     5341 gccgtcgtcg agagcgatgc ggaccccggc cgagtcgcgg acggtctcgg cggcctgcgc
     5401 gggcatcagc agcttcacgc gcgaagcctc cagggccgcc tcccggagca cctgccgcag
     5461 ccgtcggttc tccaccatga tgccgagcgg atcgtcggcc tccggagcgt cgaagacgat
     5521 tccgcccggc tccaggccgt cgctgacgcg gatggagagg atggggcagc cttcgtcctc
     5581 gagccgcccg gcgattccga tcgcctccag catccgccag gaggcgctgg cgatcgccgt
     5641 cgcccggccg tcgaaagccg gcgcgacgtt cctttccggg tcggcgggat cgatcacgat
     5701 cgccgaaagg ccgtgccggt cgagcgcgac ggccagcgtc aggccgatca ggccgccgcc
     5761 gaggatgatg acgtccgcgc ggtccatggc atcccttcca acgattttcc cgccctaccg
     5821 atgcaaagct gcaacgccaa gcgcttgacc ggagactccc gccgggcgca ttgtccgtct
     5881 caggaacatt tggggcacgg ggatggcggc ttcagcagca gcgcagagcg cccgccaggc
     5941 caggctggcg cagtggcggt cgggaatgaa gcggggcatg cgccgaagct gggcgctgct
     6001 gatcggtggg gggcttggcg cccttgcctt tcttctgctt ctggcgctgg ccagctacag
     6061 cccgaccgac ccttcgctca acacggcggc ggcgggtccg gtcaggaact gggtcggcgc
     6121 cccgggcgcg tggagcgcgg acctgctgct cagcctgttc ggaccgcccg cggccctgct
     6181 cctcccgctg atccttctga tcggcatccg gatcgcggcc ggcggcgagg gcacgcgctg
     6241 gccccgctcg ctggcgctca ccgtccttgg cctggtcctg gtcggcggcg cggcgtcgct
     6301 gctcgccggc gacgcggtca acggccttcc agcgagctgg ggcggcgcgg cgggcctgtc
     6361 gttcgcgaaa ctggtcgagc tcggtctcgc cgcgatcggc gagcccggaa tcgtcgagcc
     6421 gttccgaatc gccgccgtcg gcctgttcgc ggcgctcggg ctcgcctgct gctggttcgg
     6481 cctcgggctt cgggcggacg agcgggcctg gatcgcggcg cggcggctgc cgcgccttgc
     6541 gcgcgcctcc gccgacgacg gcgaggagct gcccgagccc gagcggcggc ccgcgcggac
     6601 gccggtcgtc gcccccgccg aaccgccgcg caccgtgatc aacgaccggg tgcgcacgcc
     6661 gccccaggcg aagcggccgc tgcgcgagcg ccaggcttcg ctcgcgctcg gcgattccta
     6721 ccagctgccc ggcctggagc tgctcgcccc ggcgcccccc ccgatcggcg gggcgctcga
     6781 caaggcgagc ctcgagcgca atgcgcgcct gctcgaatcg gtgctcgacg atttttcggt
     6841 caagggcgag atcgtcgagg tccggcccgg cccggtcgtc accatgtacg agctcgagcc
     6901 ggcgagcggc atcaaggcga gccgcgtcat tcagctcgcc gacgacatcg cccgcaacat
     6961 gtcggccctc tccgcccggg tcgccaccgt ccccggccgg agcgtcatcg gcatcgagct
     7021 tcccaatccc aagcgcgaga tggtcgtgct gtcggagctg atcgcgagcc acgccttcga
     7081 ggaccaggtg gcgcagcttc cgatcgtgct cggcaagaac atcgccggcg atccggtcgt
     7141 cgccgacctc gcgccgatgc cccacctgct cgtcgccgga accaccggct cgggcaagtc
     7201 ggtcggtctc aactgcatga tcctctccct gctctaccgg ctgacgcccg agcagtgcaa
     7261 aatgatcctg atcgacccga agatgctcga attgagcacc tacgacgaca tcccccacct
     7321 cctcgccccc gtcgtcaccg agcccgccaa ggcgatccga gcgctcaaat ggacggtcga
     7381 gcagatggag gagcgctacc ggatgatggc ttcgatcggc gtgcgccagc tctccagctt
     7441 caacgccaag gtgcgcgagg ccaaggccaa gggccagact ctcggccgcc gggtgcagac
     7501 cggctacgac cccgagaccg gccagccgaa atacgagatg gaggagctgg aatacgaggt
     7561 cctgccgcag atcgtcgtcg tcgtcgacga gctcgccgac ctgatgatga ccgccggcaa
     7621 ggaggtcgag ttcctgatcc aacgcctcgc ccagaaggcg cgtgcggccg gcatccacct
     7681 gatcatggcg acccagcgcc cctcggtcga cgtcatcacc ggagtgatca aggcgaacct
     7741 gccgacccgg atctcgttcg cggtcacgtc caagatcgat tcgcgcacca tcctgggcga
     7801 gcagggcgcc gagcagctgc tcggcaaggg cgacatgctc tacatgcccg gcggcaagca
     7861 gatcgcccgg gtgcacggcc cgttcgtctc ggacgaggag gtccgcgccg tcgccgagca
     7921 ttggaaggcc cagggccgtc cggaatatgt ccaggccgtc accgaggagc ccgaggacgg
     7981 cggctacatg ttcgagggcc agccgaccgg cgaggacgat cccgaggccc agctctaccg
     8041 caaggcggtg cagacggtgg ccgagagcca gaaggcgtcg acctcctatc tccagcgcca
     8101 gctccgggtc ggctacaaca atgccgcgcg cctgatcgag cggatggaga aagacgggat
     8161 cgtcagcgcc cccgaccatg tcggccgccg cgaagtgctg gtcgatccgg agggaaggcc
     8221 gctgtagctt cgcttcgacc
//
LOCUS       DASVQZ010000258         8293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_248831, whole genome shotgun sequence.
ACCESSION   DASVQZ010000258 DASVQZ010000000
VERSION     DASVQZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248831"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VF547_07050"
     CDS             <1..897
                     /locus_tag="VF547_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX8262613.1"
                     /translation="VEKLWGRADLPQSFGAAAAPGEPIGEIWFQDARGDEAELLVKYL
                     LTREKLSIQVHPDGQAARAAGHRRGKDEAWLVLEAEPGALIGLGLKRDISAAELRSAI
                     AAGTIEALVDWREAKAGDFYYSPAGTIHALGPGLTLVEIQENVDLTYRLFDYGRERDL
                     QVEAGVEAARPERYRAPTERREIEPGREILAEGGAFVVERWTRAAGGRLAGEVERPVW
                     LVPVRGGGLIDGQALEPPSVWLVEDQSRVRLDPGSEMLFAYPGPGVIEALFVDTRRSQ
                     PVERLDERRRYRPPVRLEPSFR"
     gene            949..1095
                     /locus_tag="VF547_07055"
     CDS             949..1095
                     /locus_tag="VF547_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262614.1"
                     /translation="MMYAFPILIALMFSYMFWLAAYRLRRDRVAVARLARQPRRAQRR
                     RAVS"
     gene            complement(1071..1847)
                     /locus_tag="VF547_07060"
     CDS             complement(1071..1847)
                     /locus_tag="VF547_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8262615.1"
                     /translation="MTVTSGDPMDDDLPEAIPAATLILMRPAAAGGPPEILMLERAET
                     MAFAAGALVFPGGRIDPDDHATARRYPHVPDAAARIAAIRETIEETGVAPALDPTPDA
                     AALRAAVAAGEPFAELLDRMGLSLDLGALTPFARWCPNFREARRFDTLFYLAAAPSGE
                     ALDSADETETVHTFWASAADVLGDIAAGRAKAIFPTRRNLERLAGFASLEEARSDAER
                     HPVRQITPWVEERAGKRFVCIPEGIGYPVTEEPYETARRR"
     gene            complement(1878..2240)
                     /locus_tag="VF547_07065"
     CDS             complement(1878..2240)
                     /locus_tag="VF547_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8262616.1"
                     /translation="MTGTKILLVEDEPLVLRNTADLLAEDGYDVTECTGCDDALARLE
                     ADPDVAVLVTDLNVTCPGDGMTLARIAAERWPHLRIVLVSGAMRPPAGTYPERAVFFT
                     KPYAPGALLHVVKSPDFA"
     gene            complement(2384..3136)
                     /locus_tag="VF547_07070"
     CDS             complement(2384..3136)
                     /locus_tag="VF547_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8262617.1"
                     /translation="MTLRTLLVDDEPLALERLRVFFRDIAGAEVVGTARDGREAAEAI
                     ERLKPDLALLDIQMPEQNGLRVAATLPPEDRPEIIFVTAFEQYAPDAFEVEAADYLLK
                     PVRFDRLWQAVERAKRRKHLRTLAGRAEAAAAPRPDPHEDGLWVPTRGGAVFVPVEAI
                     DWIEAQRDYVMLHTATRSHILRISMRALEDRLDPAELMRVHRSAFARPANVVEVQRLG
                     RTISALVLRDGAIVQVGPNYSKAVAERLKLAD"
     gene            complement(3133..4233)
                     /locus_tag="VF547_07075"
     CDS             complement(3133..4233)
                     /locus_tag="VF547_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8262618.1"
                     /translation="MARMDTPPARPGVARQAAKLTLGVWAFTFILFLMPVAAATGRLP
                     QFAIGMTAIVILFGIALSALLYWACALLKDAPTPLKVGGVFGAVCLAAFAFSLGDALL
                     GGNIIQMFMAEHRIRPDVVNRTVSNFISFSWLYGLLGTIYVILQTNAAMRERDLQLAE
                     ARTLAQTAQLTALRLQLNPHFLFNTLNAISSLIVTGRNRDGEAMLSKLCVFLRTALVG
                     DGRSEVTLGEELETLQTYLEIESIRFGDRLTVEFAAADDLLELPVPNFILQPLVENAV
                     KHGVAPTSRPVIVRVGARRDGQDLILSVSDNGGSARPGGAGTGVGLDNTRRRLEVIYG
                     PRARLETLAHDGGFTALIRLPAATAPAIGLVA"
     gene            complement(4297..5151)
                     /locus_tag="VF547_07080"
     CDS             complement(4297..5151)
                     /locus_tag="VF547_07080"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX8262619.1"
                     /translation="MKRLALVLPALCAVSLAEAAPPANVRDVVEAERAFARAARTDGV
                     NAAFLHYSAPDALVFQPGPTPAKAALAARPLPSAELSWWPVYAGIAVSGDLGFTTGPF
                     VVGSGERKGHGWYFTIWRRQPDGSWRWVLDHGPPTREPAPFAGDAEVMTLPPGRPSSR
                     NKALGSLRAAEAHLAAALALDARAALPRFLAADGRLMRVGPQPAVGAGAWAAVLAAGP
                     ERIETAPLGGEASEAGDLAYTFGTARWQKDGVPVGGHYVRIWQRREGGWKLIVDNVIA
                     SPPPRPPR"
     gene            5538..>8293
                     /locus_tag="VF547_07085"
     CDS             5538..>8293
                     /locus_tag="VF547_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX8262620.1"
                     /translation="MAAAWINEFHYDNASTDTGEYVEIAAAAGTNLAGWSIVLYNGAN
                     GQVYNTRSLSGVIPNQQNGFGTLSFAYASNGIENGSPDGIALVNNLGQVVEFISYEGT
                     FVAINGPAAGMTSTSIGAPSENGAQTGTAIGRVGTGDEASDFTWALINDDTPGGVNVG
                     QSFNTVVMDKPGAFSIGDASVIEGNSGSTPITFTVSRGTDSNVAATVNYTVNLPGGAT
                     GASASDFVSPTLSGTLSFAANEFSKTITLNVAGDLVNEPNETFTVTLSGPTGGATLAD
                     GVGDGLISNDDAAVGPGVPFINELHYDNAGTDANEGFEIAAPAGTNLAGWTLVLYNGS
                     SDSSSGSPTAATVYNTRTLAGVVPNQDDGYGTLAFTYAVNGVQNGDFDGVALVDPSGK
                     VVQFLSWEGSIVASNGPAAGMRSTDIGVFEGSSSPLGFSLQVVGSGASAADFTWVSAR
                     DDNFGAVNTDQDFIGPNATGQVRIGDASVVEGDSGTSMLVFTVTRAGGLGQAAGVDWV
                     LNLTPGGADLADLGAGQPLSGHVDFGVGVSSVTISIAVAGDTVSEGNETLNVLLANAT
                     GNIALADASGTGTILNDDPLALKIYQIQGEGHSTAYAGQPVTTSGIVTGVVGNGFYLQ
                     DPDGDGNARTSDAIFVFTGAAPAVAVGDAVSVRGTPEEFVPGAGSLSITELVTTNANV
                     VVQSHDNALPATVLIGAGGRTPPTQTFEDDGFTSYDPATDGLDFYESMEGMRVTIDSP
                     TVVSNTSSFGETWVLASGGAGATGYNGRTGIAISDGDLNPERIQIDATSALFAGYAPN
                     HSMGDKLGNVTGIMSYSFNSYEVLVSAPVTVVADVTAARETTSLTGDRDHLTIASMNV
                     ENLDPSDPQTKFDLLAQNVVYNLAAPDIIGLQEVQDANGLNGSDPLSGVVTAQKLIDA
                     IAAIG"
BASE COUNT         1318 a         2843 c         2950 g         1182 t
ORIGIN      
        1 gtcgagaaat tgtgggggcg cgccgatctc ccgcaaagct tcggcgcggc cgccgcgccg
       61 ggcgagccga tcggcgagat ctggttccag gacgcacgcg gcgacgaggc ggagctgctc
      121 gtcaaatatc tgctgacccg agagaagctg tcgatccagg ttcatcccga cgggcaagcg
      181 gcgcgcgccg cgggccatcg ccgcggcaag gacgaggcct ggctggtgct ggaagccgag
      241 cccggcgccc tgatcggcct cggcctcaag cgggacattt ccgcggccga gctgcgatcc
      301 gccatcgcgg cgggcacgat cgaggcgctg gtcgactggc gcgaagccaa agccggggat
      361 ttctattatt ctcccgcggg gacgatccat gccttgggtc cggggctgac gctggtcgag
      421 atccaggaga acgtcgatct cacctaccgg ctgttcgatt acgggcgcga gcgcgacctc
      481 caggtcgagg ccggggtcga ggcggcgcgg ccggagcgct atcgtgcgcc gacggaacgc
      541 cgtgagatcg agccgggccg cgagatactc gccgaaggcg gcgccttcgt cgtcgagcgc
      601 tggacccgcg ccgcgggcgg gcggctggcc ggcgaggtcg agcgccccgt ctggctggtg
      661 ccggtgcgcg gcggcgggct gatcgacggg caggcgctcg agccgccctc ggtctggctg
      721 gtcgaggacc agagccgggt tcggctcgat ccggggtcgg agatgctgtt cgcctatccc
      781 ggcccgggcg tcatcgaggc gctgttcgtc gatacgcgcc ggtcgcagcc ggtggagcgg
      841 ctggacgaaa ggcgccgcta ccggccgccg gtgcggctgg agcccagctt ccggtaacgg
      901 ctgggcgcat cggatggaac cgggagactc gccgagcttt gtgcggccat gatgtacgcg
      961 ttcccgatcc tcatcgccct gatgttcagc tacatgttct ggcttgccgc ctatcggctt
     1021 cgacgcgacc gcgtcgcggt cgcccggctc gcgcgccagc cgcggcgggc tcagcggcgc
     1081 cgcgcggttt cgtagggctc ctccgtcacc ggatagccga tcccctcggg gatgcagacg
     1141 aagcgtttgc cggcgcgctc ctccacccag ggggtgatct gccgcaccgg atggcgctcg
     1201 gcgtccgagc gcgcttcctc cagcgaggcg aagccggcga gccgctcgag gttgcgccgg
     1261 gtggggaaga tcgccttggc gcggccggcc gcgatgtcgc cgagcacgtc ggcggcgctg
     1321 gcccagaagg tgtggaccgt ctcggtctcg tccgcgctgt cgagcgcttc gcccgagggc
     1381 gccgcggcca gatagaagag ggtgtcgaag cggcgcgcct cgcggaaatt ggggcaccag
     1441 cgggcgaacg gggtcagggc ccccagatcg agcgagagac ccatgcggtc gagcagctcg
     1501 gcgaacggtt cgcccgccgc cacggctgcc cggagggcgg cggcgtcggg cgtggggtcg
     1561 agcgccggcg cgactccggt ctcctcgatc gtctcgcgga tggcggcgat ccgggcggcc
     1621 gcgtccggga cgtgcggata gcggcgcgcg gtggcatgat cgtccggatc gatccgcccg
     1681 cccgggaaga cgagggctcc ggcggcgaag gccatggtct cggcccgctc cagcatcagg
     1741 atctcgggcg gcccgccggc cgcggccggt cgcatcagga tgagggtcgc ggcggggatc
     1801 gcctcgggca ggtcgtcgtc catcggatcg ccactagtga cggtcaccgg tgactgtcac
     1861 caggcaggtc ccgaggccta ggcgaaatcg ggcgacttga cgacgtggag cagcgcgccc
     1921 ggagcatagg gcttggtgaa gaagacggcg cgctcgggat aggttccggc cggcgggcgc
     1981 atagcccctg aaaccaggac gatgcgcaga tgcggccagc gctccgcggc gatccgggcc
     2041 agggtcatgc cgtcgccggg acaggtgacg ttgaggtcgg tgacgagcac cgcgacgtcc
     2101 gggtcggcct ccagccgggc cagcgcatcg tcgcagccgg tgcactcggt gacgtcgtag
     2161 ccgtcctcgg cgagcaagtc ggcggtgttg cgcaggacga gcggctcgtc ctcgaccagc
     2221 aatatcttgg tccctgtcac gcggaacctt cccctgttgt ccggcgaggg aatacaggcc
     2281 ggcaaggcgc taacccagat caggccccga cgttccccgc gcgactccgg aaattcaaag
     2341 aaagctgggg atttgtccgg aagagggacg ggccgccgtc tcctcagtcg gcgagcttga
     2401 gccgctccgc caccgccttc gagtagttcg ggccgacctg gacgatggcg ccgtcccgca
     2461 ggacgagggc gctgatcgtc cggcccaggc gctggacctc gacgacattg gccgggcgcg
     2521 cgaaggcgga ccggtggaca cgcatcagct cggccgggtc cagcctgtcc tcgagcgccc
     2581 gcatcgagat gcgcaggatg tggctgcggg tggcggtgtg gagcatgaca tagtcgcgct
     2641 gcgcctcgat ccagtcgatc gcctcgaccg gcacgaacac ggcccctccg cgcgtcggca
     2701 cccagaggcc gtcctcgtgc gggtcggggc gcggcgccgc cgcggcttcg gcccggccgg
     2761 cgagggttcg aagatgcttg cggcgcttgg cgcgctcgac cgcctgccac agccggtcga
     2821 agcggaccgg cttgagcaga tagtcggccg cctcgacctc gaaggcgtcg ggagcatatt
     2881 gctcgaaggc ggtgacgaag atgatctcgg gccggtcctc gggcgggagg gtggcggcga
     2941 cccgaaggcc gttctgctcg ggcatctgga tgtcgagcag ggcgaggtcc ggcttcagcc
     3001 gctcgatcgc ctcggccgct tcccggccgt cgcgcgcggt tccgaccacc tccgcgccgg
     3061 cgatgtcgcg gaagaacacc cgcagccgct cgagggcgag cggctcgtcg tcgacgagga
     3121 gcgtgcgcag ggtcatgcca ccagcccgat cgccggcgcc gtcgcggccg ggaggcggat
     3181 gagcgcggtg aagccgccgt catgggcgag cgtctcgagc cgggcccgcg ggccgtagat
     3241 cacctcgagc cggcggcggg tattgtcgag gccgacgccg gtgccggcgc cgcccggccg
     3301 ggccgagcct ccgttgtcgc tgaccgagag gatcagatcc tggccgtcgc ggcgggcgcc
     3361 gacgcggacg atgaccggcc ggctggtcgg ggcgaccccg tgcttcaccg cattctcgac
     3421 cagaggctgg aggatgaagt ttggcaccgg cagctcgagc aggtcgtcgg cggccgcgaa
     3481 ctcgacggtg agccggtcgc cgaagcggat cgactcgatc tcgagatagg tctgaagcgt
     3541 ctccagctcc tcgccgagcg tgacctcgct gcggccgtcc ccgaccagcg ccgtcctcag
     3601 gaagacgcac agcttcgaga gcatcgcctc gccatcccgg ttgcggccgg tgacgatcag
     3661 cgaggagatg gcgttgagcg tgttgaacag gaaatgggga ttgagctgga gcctgagcgc
     3721 ggtgagctgg gcggtctggg cgagggtgcg cgcctcggcg agctggaggt cgcgctcccg
     3781 catcgcggcg ttggtctgga ggatgacata gatggtgccc agcaggccgt agagccacga
     3841 aaagctgatg aaattggaga cggtccggtt gacgacgtcg ggccggatcc ggtgctcggc
     3901 catgaacatc tggatgatgt tgccgccgag cagcgcatcg cccagcgaga aggcgaaggc
     3961 cgcgaggcag acggccccga agacgccgcc gaccttgagc ggggtcggag cgtccttgag
     4021 aagcgcgcag gcccagtaga gcagggccga caaggcgatc ccgaaaagga tgacgatcgc
     4081 cgtcatcccg atcgcgaact ggggcagccg tccggtcgcc gcggccaccg gcatcaggaa
     4141 caggatgaag gtgaaggccc acactccgag cgtcagcttc gccgcctggc gggcgacgcc
     4201 ggggcgggcg ggcggcgtat ccatcctcgc catcctacag ctccggcgcc cgccgcgccg
     4261 cccttttcgt cgcagcgacg gcgggttttg cagaactcac cgcggcggac gcggcggcgg
     4321 ggaggcgatg acgttgtcga cgatcagctt ccagccgccc tcgcgccgct gccagatgcg
     4381 gacatagtgg ccgccgaccg gcacgccgtc cttctgccac cgcgcggttc cgaaagtgta
     4441 ggcgagatcg ccggcctccg aggcttcccc gccgagcggg gcggtctcga tccgctcggg
     4501 tccggcggcg agcaccgccg cccatgcgcc ggcgccgacg gcgggctgcg ggccgacccg
     4561 catcagccgt ccgtccgcgg cgaggaagcg gggcaaagcg gcgcgggcgt ccagggcgag
     4621 cgcggccgcg agatgcgcct cggccgccct caggcttcca agggccttgt tccggctgga
     4681 cggccggccg ggcggaagcg tcatgacctc ggcgtcgccg gcgaagggcg ccggctcgcg
     4741 cgtcggcggg ccgtggtcga gcacccagcg ccagctgccg tccggctggc gcctccagat
     4801 ggtgaaatac cagccgtggc ccttgcgctc gccgctgccg acgacgaacg ggccggtggt
     4861 gaagccgagg tctccggaaa cggcgatgcc ggcatagacc ggccaccagc tcagctcagc
     4921 ggacggcagc ggcctggccg cgagcgccgc cttcgcgggc gtcggaccgg gctggaagac
     4981 gagggcgtcg ggcgccgagt aatggaggaa ggcggcgttg acgccgtcgg tccgcgccgc
     5041 tcgggcgaag gcgcgctccg cctcgacgac gtcccggacg ttcgccggcg gcgccgcctc
     5101 ggcgagcgag acggcgcaca aggcggggag gacgagggcg agacgtttca tcgggggcgg
     5161 ctttcgaaag ggcggtacgc cgcgggagag gcgagagggg ggcgtggccg cctcccgcgg
     5221 cgatgggccc gggataggcg agggcccgga cgcgggctcg acgcctgcga ccgccccgcg
     5281 gcttgaggcg gcgaagcggc gccggcgcga ggaaagcgtc ccctcgaacg acgaaaatcc
     5341 gaatttgtcc ctttttggaa cttatcccga aattacaacc gtttacggca cttatatcca
     5401 atcatgacat gcgcttggca caaaaagctg ctatggtccg attcggctga ttcttatcgg
     5461 ccctcttcac acacgcacgt gaaggattgc cacgacgcgc ggggcggcgt ggcctcgacc
     5521 tgtttagggg ggatgctatg gccgcggcct ggatcaacga attccattac gacaatgcta
     5581 gcaccgatac tggcgaatat gtcgagatcg ccgccgcggc cggcaccaac ctcgccggct
     5641 ggtcgatcgt cctctacaac ggcgccaacg gccaagtgta caacacccgc agccttagtg
     5701 gcgtaattcc aaaccagcag aacggattcg gaaccctctc cttcgcttat gcgagcaacg
     5761 gcatcgagaa cggatcgccg gatgggatag cgctcgtcaa caatctcggt caggtcgtcg
     5821 agttcatcag ctatgaaggc accttcgtag caatcaacgg cccggcggcc ggaatgacca
     5881 gcaccagcat cggcgcgccc agcgagaacg gcgcccaaac cgggaccgcg atcggccggg
     5941 tcggcaccgg cgacgaggcg tccgatttca cctgggcgct gatcaacgac gacacccccg
     6001 gcggcgtcaa tgtcggtcag tcgttcaaca ctgtggtcat ggacaagccc ggcgccttct
     6061 cgatcggcga tgccagcgtc atcgagggca atagcggctc gaccccgatc accttcacgg
     6121 tcagccgcgg gacggattcg aacgtcgccg ccacggtcaa ttacaccgtc aacctgcccg
     6181 gcggcgccac cggcgccagc gcgtccgact tcgtctcgcc gacgctgagc ggcacgctca
     6241 gcttcgcggc gaatgagttc agcaagacga tcacgctcaa cgtcgcgggc gacctcgtca
     6301 atgagccgaa cgagacgttc acggtcaccc tttccggccc gaccggcggc gcgaccctcg
     6361 ccgacggcgt cggcgacggc ctcatcagca acgacgatgc ggcggtcggc cccggcgtgc
     6421 cgttcatcaa cgagctccat tacgacaatg ccggcacgga cgcgaacgag gggttcgaga
     6481 tcgccgcgcc ggccggcacc aacctcgcgg gctggacctt ggtcctctac aacggcagct
     6541 ccgacagcag ctcgggaagc ccgaccgcag cgacggtcta caacacccgc actctggcgg
     6601 gagtcgttcc gaaccaggat gacggttacg gcacgctcgc cttcacctat gccgtcaacg
     6661 gagtccagaa cggtgacttc gacggcgtgg cgctggtcga tccgagcggc aaggtcgtgc
     6721 aattcctctc ctgggaggga agcatcgtgg cctccaacgg ccccgcggcc ggaatgcgca
     6781 gcaccgacat cggagtcttt gagggcagct cctcgccgct cggcttctcg ctgcaggtgg
     6841 tcggttccgg cgccagcgcc gccgacttca cctgggtttc ggcgcgcgac gacaatttcg
     6901 gcgcggtcaa caccgaccag gacttcatcg gccccaacgc gaccggccag gtccggatcg
     6961 gcgacgccag cgtcgtcgag ggcgacagcg gcacttcgat gctcgtcttc accgtcactc
     7021 gcgccggcgg cctcggccag gcggcgggcg tcgactgggt gctcaacctg acgccgggcg
     7081 gcgccgacct cgccgatctc ggcgccggcc agccgctttc cggccatgtc gacttcggag
     7141 tcggcgtgtc gtcggtgacg atctccatcg ccgtcgccgg cgatacggtg agcgagggca
     7201 acgagacgct caacgtcctg ctcgccaacg cgaccgggaa catcgccctc gccgacgcct
     7261 cggggaccgg aacgatcctc aacgacgacc cgctcgcgct caagatctac cagatccagg
     7321 gcgagggcca ctcgaccgcc tatgcgggcc agccggtcac caccagcggc atcgtcaccg
     7381 gcgtggtcgg caacggcttc tacctccagg atccggacgg cgacggcaat gcgcgcacct
     7441 cggacgcgat cttcgtgttc accggcgccg ccccggccgt cgcggtcggc gacgccgtct
     7501 ccgtgcgggg cacgcccgag gagttcgttc cgggcgccgg cagcctcagc atcaccgagc
     7561 tcgtcacgac caacgccaac gtcgtcgtcc agtcgcacga caatgcgctc ccggcgaccg
     7621 tgctgatcgg cgccggcggc cgcacgccgc cgacccagac cttcgaggac gacggcttca
     7681 ccagctacga tccggcgacc gacggcctcg acttctacga atcgatggaa gggatgagag
     7741 tcaccatcga ctccccgacc gtggtgtcga acacgtcgag cttcggcgag acctgggtgc
     7801 tcgcctccgg cggcgccggc gccaccggct ataacggccg caccggcatc gccatctccg
     7861 acggcgacct caatccggag cggatccaga tcgacgccac cagcgccctg ttcgcgggct
     7921 atgcgccgaa ccattcgatg ggcgacaagc tgggcaacgt caccggcatc atgtcgtaca
     7981 gcttcaacag ctacgaggtg ctggtcagcg cgccggtcac cgtcgtcgcc gacgtcaccg
     8041 cggcgcgcga gacgaccagc ctcaccggcg accgcgacca tctcaccatc gccagcatga
     8101 acgtcgagaa tctcgatccc agcgatccgc agaccaagtt cgacctgctc gcccagaacg
     8161 tcgtctacaa cctcgcggcg ccggacatca tcggcctgca ggaggtccag gacgcgaacg
     8221 gcctcaacgg cagcgacccg ctctcgggcg tcgtcaccgc gcagaagctg atcgacgcga
     8281 tcgcggcgat cgg
//
LOCUS       DASVQZ010000259         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_153884, whole genome shotgun sequence.
ACCESSION   DASVQZ010000259 DASVQZ010000000
VERSION     DASVQZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153884"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1369
                     /locus_tag="VF547_07090"
     CDS             101..1369
                     /locus_tag="VF547_07090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8262621.1"
                     /translation="MNAPPRGRHLVSADRGFATGGGMLARLLGGSFHRILDRVEAGLA
                     KGAIEATLPDGSTRRLGGRAPGPEPVVRLHSWNALVRLMTSGSVGWYKAWEQGEWSSP
                     DPVPLFELFMLNGETLGNTGRAKGLFRLVNRLAHRFRDNDKQGARENISHHYDLGNDF
                     YRAWLDSGMTYSSAIFAEPIGAEEPLEAAQERKLRRLLDRLALRPGQHLLDIGCGWGS
                     LAETAARGYGVTVTGLTLSAAQKDFADRRLAAAGLGGRCAIELTDYRDAVGQYDAVAS
                     VEMAEAVGQDYWPDYLGSIARLLKPGGRAALQFISIREPLFEDYAANADFIQTYIFPG
                     GMLVSEARFRALAEAAGLAWQDRDGFGLHYAETLRRWRGRYEEAVAEGRLPAGFDEPF
                     HRLWRYYLLYCEGGFRGAGIDVAQVTLVKG"
     gene            complement(1479..2192)
                     /locus_tag="VF547_07095"
     CDS             complement(1479..2192)
                     /locus_tag="VF547_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8262622.1"
                     /translation="MSDKFLHGKIALVTGASRGIGAATAEALASAGAHVVLAARTAAD
                     LERVEDSIHRAGGTATIAPLDLIETDSIARLAAAVAERWSRLDILMLNAATLGSLTPV
                     AQIDGKEFSRVLTLNLLASQALIAGFDPLLRKSEAGRLLAVTSSVGRRPRAYWGAYGA
                     SKAALETLALAYAEEVRNISAVRVAMIDPGSTATQMRARAYPGENPATLKPPAAVAEA
                     VVAMLERDFETGERLEVSA"
BASE COUNT          323 a          805 c          807 g          343 t
ORIGIN      
        1 caggcccttg tctccaaggg ccagagctgg atggtgacca aggagcccca cgcgatctga
       61 gcggattgcg cgggcggccg gaacgcgcca cctagcggcc atgaacgcac cgccccgcgg
      121 ccgccatctc gtctcagccg atcgcggctt cgcaaccggc ggtgggatgc ttgcgcgcct
      181 gctgggcgga agcttccatc ggattctcga ccgggtcgaa gccgggctgg cgaagggggc
      241 gatcgaggcc accttgccgg acggctccac gcgccggctc gggggacggg cgccggggcc
      301 cgagccggtg gtaaggctcc acagctggaa cgcactcgta cgcctcatga cctcgggttc
      361 ggtgggctgg tacaaggcgt gggagcaggg ggaatggtcg agccccgatc cggtgccgct
      421 gttcgaattg ttcatgctga acggggagac gctcggcaat accggccgcg ccaaggggct
      481 gttccggctg gtcaaccgcc tcgcccaccg cttccgcgac aacgacaagc aaggcgcgcg
      541 cgagaacatc tcgcaccatt acgacctcgg caacgacttc taccgggcct ggctcgattc
      601 cggaatgacc tattcgagcg cgatcttcgc agagccgatc ggcgccgagg agccgctcga
      661 ggccgcccag gagcgcaagc tccgccggct cctcgaccgg ctcgcccttc ggccggggca
      721 gcatctgctc gacatcggct gcggctgggg ctcgctcgcc gagaccgcgg cccgcggtta
      781 cggggtgacg gtcaccggcc tcaccctgtc cgcggcgcag aaggatttcg cggatcggcg
      841 gctggcggcc gcgggcctcg gcggccgctg cgcgatcgag ctgaccgact atcgcgacgc
      901 cgtcggccaa tatgacgccg tcgcgagcgt cgagatggcc gaggcggtcg gccaggacta
      961 ttggcccgac tatctcggca gcatcgcccg actgctgaag cccggcgggc gcgccgcgct
     1021 ccagttcatc tcgatccgcg agcccttgtt cgaggattat gcggccaatg ccgacttcat
     1081 ccagacctac atcttcccag gcgggatgct ggtcagcgaa gcccggttcc gcgcgctcgc
     1141 cgaggccgcc ggcctcgcct ggcaggaccg cgacggcttc ggcctccatt atgccgagac
     1201 cctgcggcgc tggcgcggcc gctacgagga ggcggtcgcc gaggggcggc tccccgccgg
     1261 cttcgacgag cccttccacc gcctgtggcg ctattacctc ctgtattgcg agggcggctt
     1321 ccgcggcgcc ggcatcgacg tcgcgcaggt gaccttggtg aagggctgac ccccctccct
     1381 gcacgcaggg aggggccggg ggtgggtgcg tctccgaagg atcattgcag gcggagacca
     1441 acccacccct gacccctccc tgtacccagg gagggggatc aggcgctcac ctcgagccgc
     1501 tccccggtct cgaagtcccg ctcgagcatc gccaccaccg cctccgccac cgcggccgga
     1561 ggcttcagcg tcgccgggtt ctcgcccgga taggcgcggg cgcgcatctg ggtggcggtc
     1621 gagcccgggt cgatcatcgc gacccggacg gcggagatgt tgcggacctc ctccgcataa
     1681 gcaagggcga gcgtctccag cgcggccttg gaggcgccgt aggcgcccca ataggcgcgc
     1741 gggcggcggc cgacgctgga ggtcaccgcc agcagccgtc ccgcctcgct cttcctcagc
     1801 agcggatcga agccggcgat cagcgcctgg ctcgccagca ggttcagggt cagcacccgg
     1861 ctgaattcct tgccgtcgat ctgggcgacc ggggtcagcg agccgagggt ggcggcgttg
     1921 agcatcaata tgtcgagcct gctccatcgc tcggcgaccg ccgccgccag ccgggcgatg
     1981 ctgtcggtct cgatgaggtc gagcggcgcg atcgtcgccg tgcccccggc ccggtggatc
     2041 gagtcctcga cgcgctccag gtcggcggcg gtgcgggcgg cgaggacgac gtgcgcgccc
     2101 gcgctcgcca gcgcttccgc ggtggcggcg ccgatgccgc ggctggcgcc ggtgacgagg
     2161 gcgatcttgc cgtggaggaa tttgtccgac atctttgcct gcctcctaga ccgtcatggc
     2221 gagcgaagcg aagcaatcca gcggcccagg cgccgaggct ggattgcttc gtcgctgc
//
LOCUS       DASVQZ010000260        16582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_822954, whole genome shotgun sequence.
ACCESSION   DASVQZ010000260 DASVQZ010000000
VERSION     DASVQZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16582
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822954"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VF547_07100"
     CDS             <1..709
                     /locus_tag="VF547_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8262623.1"
                     /translation="GEAAARKFVSGGWRAIGTGRRGDRLKRLQEELGEAFLPLELDMR
                     DSDALARFGEDWPEVFGHIDLLINNAGLAPPLETLQESQLAPALQAIETNVTGLVVLT
                     RRLLPTLIERKGAIINLSSVAATYPYRGGAVYGGTKAFVRQFSLDLRSDLHGTGVRVT
                     SIEPGMAETEFTLVRTGGDQAASEALYRGMNPMTAEDIAETLFWVATLPRHLNINALE
                     LMPVSQSFAGFQVAREG"
     gene            complement(726..1544)
                     /locus_tag="VF547_07105"
     CDS             complement(726..1544)
                     /locus_tag="VF547_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262624.1"
                     /translation="MSYSRREILAAAGGAGLMIYWGRGSELHAQSSPAQRISALAATS
                     AIRTYHWLDRGRAPRGYTKGMAVAFGRTYCKWKRGDPIARRIAAAASQDGQTDALAWY
                     SATFSSLAMSNSTAGADVLRHVFALLMGLGMRESSGQHCEGRDMSANNVSAATAEAGL
                     FQVSFNSIDAHPGLRSLFQAYQGSTDFSDIFGEGVTCGASSWRDWGTGEGKEFQRLTK
                     ACPAFAVEYAALGLRVMRRHWGPLNTRKAEVRRDADSLFRSVQTLIDSEGITEV"
     gene            1681..2142
                     /locus_tag="VF547_07110"
     CDS             1681..2142
                     /locus_tag="VF547_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8262625.1"
                     /translation="MTDIASRLAELGIELPAPAAPVASYVPTVLAGDLLHISGQVSFE
                     KGELLTGRLGEDRDLEFGIRAARACGLMLLAQIEKAVGLGRVERIVKLGVFVNSAGSF
                     TDQPKVANGASELMQDLFGEAGRHARSAVGVPVLPLGAAVEIDAIVQIKAL"
     gene            2114..2848
                     /locus_tag="VF547_07115"
     CDS             2114..2848
                     /locus_tag="VF547_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX8262626.1"
                     /translation="MRSSRSRRSELERLGAVPFAHRGLHGAGLVENSGAAIRAALAGG
                     YGIELDVQLSADGEAMVFHDYELDRLTGDRGLLLRRTAAELAAIRFAAADEAMPRLAE
                     ALRIVSGRGPLLIELKSRERRVERLCRAVADALAGYEGPVGAMSFNPQVGAWFARNAP
                     QVLRGLVVTEQGKKGLRGRIERRLFLWRARPDFLAYDIRDLPSRFAGRARACGLPVYA
                     WTVRTGADRARAAAHADQIIFEAAPG"
     gene            2845..3975
                     /locus_tag="VF547_07120"
     CDS             2845..3975
                     /locus_tag="VF547_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8262627.1"
                     /translation="MSEMVARLGQGVVSFEPADWDRCAGTGDPFLSHAFLSALEESGS
                     ATARTGWQSVPIAIDGADGIPAAVMPAYVKSHSQGEYVFDHGWADAWERAGGDYYPKL
                     QIAVPFTPVPGRRLLARREELAPGLIAAAEAVVRQHRLSSAHATFIAEREVPLFERAG
                     WLIRIDSQFHWHNRGYGGFEDFLADLSSRKRKALRRERAAALAGLTVEHVTGAAIAEA
                     HWDSFWAFYQDTGSRKWGRPYLTRKFFSLLGERMADRILLIFALRDGRPIAGALNLIG
                     EEALYGRYWGTIEEVPNLHFEICYHQAIEAAIGRGLSRVEAGAQGPHKLSRGYAPVPT
                     FSAHFIPDPDFRRAVADFLAAERRAVAREIEALAGMTPFRKG"
     gene            4142..6184
                     /locus_tag="VF547_07125"
     CDS             4142..6184
                     /locus_tag="VF547_07125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8262628.1"
                     /translation="MIRKAFAVLLLLAAPAAAADGPSRILEARDLFSLEAASDPQISP
                     DGSKIAYVRRSGDIMTDRMRPTIWLVDARSGEQVPLAAGPGSHSQPRWSPDGRRLAYI
                     STAEGGAAQLFVRWMGSGESVRITGLPDTPTSIAWSPDGRQIAYTMFVPGDGLKLGAL
                     PPKPEGAQWAPPLEIHSDVTYRTDEEGYLKPGFSHVFLVAADGGAPRQLSFGSVHDNG
                     PLSWTPDGRTLLFSSNRSPNWAREPLNTEVYALDLASGAISALTRRNGPDAEPVASPD
                     GRLIAYTGFDDKERSYENALLYVMERDGGSPRALTASLDRSVDSPRWAADGRSIYVAY
                     EDGGTRKVARVGLDGSIRTVAEGLSGASLDRPYTGGSFTVARDGAVAAPSGSAQRPPD
                     VALYRAGAKRQLTRLNAELLEPKRLGEVRHLRAVSFDKRPIDAWLTLPPGRAEGQKVP
                     LILEIHGGPFAAYGPHFSTDNQLYAAAGYAVLSVNPRGSTSYGAEFANLIHHAYPGRD
                     YDDLMAAVDEAIAGGYADPDKLFVTGGSGGGVLTAWIVGKTDRFKAAATQKPVIDWTS
                     FALTADSPAFFSRYWFGKYPWEDHESFWIRSPLSLVGNVRTPTLVVVGSQDYRTPVSE
                     AEQYYTALQLRGVPTALVKVPGASHGGIAARPSQAAAKAAAILAWFEKYKDGAPAK"
     gene            complement(6213..6512)
                     /locus_tag="VF547_07130"
     CDS             complement(6213..6512)
                     /locus_tag="VF547_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEX8262629.1"
                     /translation="MGNSLKSATFLIESRIADAAMGDPDALYELGVAYSTGTHGLEVD
                     LIEAHKWFNLAALNGSERGQECRAEIAEEMTAREIAEAQRQARAWLSDTMVRRAA"
     gene            complement(6726..7571)
                     /gene="panC"
                     /locus_tag="VF547_07135"
     CDS             complement(6726..7571)
                     /gene="panC"
                     /locus_tag="VF547_07135"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX8262630.1"
                     /translation="MQTVRDIETLRAAVAELRAQGATIAFVPTMGALHAGHMALVAEG
                     RRRADHVVASIFVNPTQFAANEDLSTYPRREATDGKMLEEEGCAILWAPDAATMYPDG
                     FETDVRAGSLGEGLDGAARPGHFDGVATVVSRLFEQVRPDIALFGEKDYQQLAVIRQM
                     VRDRGLAVEIVAVPTQRDADGLALSSRNAYLTEEERQAARALPRALGEAAASIQRGGS
                     VAEALATAKERLTKAGFDPIDYVELCDAETLDPVTRVDRPARLLAAARIGRTRLIDNL
                     AVEPQ"
     gene            7644..8447
                     /locus_tag="VF547_07140"
     CDS             7644..8447
                     /locus_tag="VF547_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX8262631.1"
                     /translation="MASPHFIVFANEKGGTGKSTTAVHSAVALAAAGRRVAAVDLDTR
                     QRTLGRYLDNRAETVKRLGIDLVMPAYETFDPDKGEPIEAQLDRIAADVEIVVVDTPG
                     RDDPHARSAMIRADTLVTPINDSFVDLDLIGQVDAETYRVRRPSFYAELVWNSRTQRA
                     KAHGASVDWVVLRNRMQHIEARNMRRVGEALTELSRRVGFRVIPGLGERVIYRELFPK
                     GLTLLDLPHLGEVGIAHIAARQELREMIAGFALPQPGEAAPARAAALAG"
     gene            8452..8868
                     /locus_tag="VF547_07145"
     CDS             8452..8868
                     /locus_tag="VF547_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX8262632.1"
                     /translation="MGLLILLAAAGLLWAFATGRLKGFTYEDGIAAASFLLGLRLFTT
                     GRLILGSALMAGALLWAAWRRQRPSTKPMPAEDARRLLGVGEGATLQEIRDAHRRLIA
                     KVHPDAGGSAELAHRVNAARDTLLAETMNRKPPRAS"
     gene            8898..10280
                     /locus_tag="VF547_07150"
     CDS             8898..10280
                     /locus_tag="VF547_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX8262633.1"
                     /translation="MSHSLHPTSLREYDIRGIYGESLAEEDARAVGRTFGTLVRRGGG
                     GRVAVGRDGRMSSPGLESALIEGLTGSGVDVVRIGLGPTPMLYFAEAELPVDGGIMIT
                     GSHNPGNYNGFKMVLGHGAFFGRRIQELGGMAAAGDWEQGSGSVRDEDVMERYVERLL
                     QGFDGGPFRIGWDCGNGAAGPVVERLVKRLPGEHFTLFTEVDGRFPNHHPDPTVESNL
                     EDLKRLVRDERLDFGVAFDGDADRLGAIDGQGRVVWGDQLLSILAEPVLREVPGATII
                     ADVKASQALFDRVAELGGTPLMWKTGHSLIKSKMRDTGAPLAGEMSGHIFFKHEWYGF
                     DDAIYAAIRLIRAVAQLGGSLTALRSAMPPMINTPEMRFQVDESRKFPVVEEVLERLS
                     ASGAQVDRTDGARVSTPDGWWLLRASNTQDVLVARAEAKDEAGLERLVAQIDEQLALS
                     GLQRGEQVAH"
     gene            10280..10528
                     /locus_tag="VF547_07155"
     CDS             10280..10528
                     /locus_tag="VF547_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262634.1"
                     /translation="MPRLYPEALLFCAAWAALAIYGGLELGWKAGAVLTLGLFILIMP
                     TSAVILSRTGSFKLERGVRWALLTVAAAVLLSALNNQG"
     gene            complement(10535..11536)
                     /locus_tag="VF547_07160"
     CDS             complement(10535..11536)
                     /locus_tag="VF547_07160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEX8262635.1"
                     /translation="MARLGSWIEPFPEGLYIRPAEAWVDPNLPKARALVTHGHADHAR
                     GGHGEVFATPETLAIMECRYGPQAGGRATPYGEVVRLGDVEASFVPAGHVLGSAQIVL
                     EHGGERVVVSGDYKRRADPTCPPFQPVPCDVFITEATFGLPVFRHPATASEIDRLLGR
                     LHGNPDRCVLVGAYALGKAQRLIMELRGRGHREPIYIHGALQRLCDLYRDHGIDLGDL
                     RPATGAGKEELRGKIVVAPPGCLNDRWSRRLPDPITAMASGWMRVRQRARQRNVELPL
                     IVSDHADWDELTGTIEELRPREVWITHGREDALMHWCMTRQIKARELNLVGREDEDD"
     gene            11603..11797
                     /locus_tag="VF547_07165"
     CDS             11603..11797
                     /locus_tag="VF547_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262636.1"
                     /translation="MDLAGGQWAILNIVGPLLLAAVLLWALLRNRRSRRNGSGTTEQA
                     TRDLYREEEEKRRSGDDGRS"
     gene            11781..14207
                     /locus_tag="VF547_07170"
     CDS             11781..14207
                     /locus_tag="VF547_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEX8262637.1"
                     /translation="MTGAAEPLLPAAVDAWFAAKGWQPRRHQLEMLDAARTGRSALLV
                     APTGAGKTLAGFLPSIVELIEAPTDGLHTLYISPLKALAVDVQRNLLTPIGEMDLPIR
                     VETRTGDTPADRKARQRARPPQMLLTTPESLSLLLSHEDSALLFETLATVVVDEIHAF
                     ATGKRGDLLSLALSRLQRLSPRLRRVGLSATVSDPEAFRGWLAPHGDADMVELVEGEP
                     GAPAQLAIMLPEEDKIPWAGHSGRWAALSVMREIERHKTTLLFCNTRSLAELIFQDLW
                     AVNEHHLPIGIHHGSLALEARRKVENAVAEGRLRALVCTASLDLGVDWGDVDLVIQMG
                     APKGSSRLMQRIGRANHRLDEPSEAIIVPGNRFEYLEARAALDAIEEGALDAENFRPG
                     ALDVLAQHIMALACAAPFREEEMLEEVRSAAPYAGLREETFLAILSFIESGGYALRAY
                     DKFRRIARDSDGLWRVSHPRFIQQHRMNAGIIVDTPLYYVRFRNGRVLGTVEDNFAET
                     LSPGDTFFFSGLVLEVERIDGIDLIVHATSKSARIPTYMGARIAISTNLADRVRGFLH
                     DRRQWERFPDPVRDWLRVQEYRSAIPAPDQLLVETFPHERIHYMAAYSFEGWNAHQSL
                     GMLITRRMESSGLKPLGFVANDYAIACYGLEPILDPAPLFSADILEKEFVDWVEGSHL
                     LKRAFREVAVIGGLVERHHPGRRKTGKQVTFSTDLIYDVLRRYEPDHLLLKAAWADAR
                     EKMTDVGRLARLLDRAQRTMLHVELDRVSPMAVPVLTIIGRERMPQGTVDDQLLIEAE
                     SLAATAMRID"
     gene            complement(14348..15169)
                     /locus_tag="VF547_07175"
     CDS             complement(14348..15169)
                     /locus_tag="VF547_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8262638.1"
                     /translation="MTSGDPGGTSGAERQGWWLPWLLFAAIGGINAAVNATSVVDDRF
                     RLGQPVPAWQPWTWELTSLGAWLALAPVVFAASAQLRPPRFRWPAALALHALLSVAVS
                     LAHVGFMMTARMAIYAALDGTYRFAGSVPGVLVYEYRKDLVTYLLLALLFALARRLAP
                     PAPAAAPGGGFRLEVRDGGRTAWLAPEDIEWAQAAGNYVELHGRFGERLHRETLASLE
                     ALLAPHGFRRIHRSRLVRAAAVKEIETKASGDFELVMESGERLAGSRRFRARLQG"
     gene            15279..16463
                     /locus_tag="VF547_07180"
     CDS             15279..16463
                     /locus_tag="VF547_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX8262639.1"
                     /translation="MQDRGSGERRFGLDWLRIGAFALLILYHIGMFFVPWDWHVKTSR
                     PQEWLELPMLALNPWRLALLFVVSGVASRVLLARMAGPGAFAALRSRRLLIPLAAGMA
                     LFVAPQPWAQLQEQAGYAQGFWHFWTRDYFEFGGSLGLILPTWNHLWFVAYLWLYTMA
                     LALLAGLPPTARASVQRAFERLFEGRRLLWLPILYFWLLRIGLYPAFGETHALLDDPY
                     AHAFYGAAFFFGVGLARADGLWLRLVAGWQPAAWLALAGYGAIVLLDLTIPGESGPAE
                     LLAARMARSVQAWATILALLGFAQRHLHRDGPARRYLTEAIFPWYIAHQTIIVLAGHW
                     LRPLGLGPGVEFAILLAATLAGCAATYEIGRRIAWLRPLLGLRPGQRAAAPAPSAAAA
                     AP"
BASE COUNT         2394 a         5981 c         5695 g         2512 t
ORIGIN      
        1 cggcgaagcg gcggcgcgca agttcgtgag cggcggctgg cgggcgatcg gcacgggacg
       61 ccgcggggac cggctgaaaa ggcttcagga ggagctcggc gaagcgttcc tgccgctgga
      121 gttggacatg cgcgacagcg acgccctcgc ccggttcgga gaggattggc ccgaggtctt
      181 cggccatatc gacctgctga tcaacaatgc cggcctggcg ccgccgctcg agaccctcca
      241 ggaatcgcag ctcgctccgg cgctgcaggc gatcgagacc aacgtcaccg ggctcgtcgt
      301 cctcacccgc cggctgcttc cgaccctgat cgagcgcaag ggcgcgatca tcaacctgag
      361 ctcggtggcg gcgacctatc cgtaccgggg cggcgccgtc tacggcggca ccaaggcgtt
      421 cgtccgccag ttctcgctcg accttcgctc cgacctccac ggcaccggcg ttcgggtcac
      481 ctcgatcgag cccggaatgg ccgagaccga gttcaccctg gtccggaccg gcggcgacca
      541 ggcggcgtcg gaggcgctct accgcgggat gaacccgatg accgccgagg acatcgccga
      601 gaccctgttc tgggtcgcga ccctgccgcg ccacctcaac atcaacgcgc tggagctgat
      661 gccggtcagc cagagcttcg ccggcttcca ggtggctcgg gagggctgat cgcccggccg
      721 cccgctcaga cctcggtgat cccctccgaa tcgatcaggg tctgaacgga tcggaacagc
      781 gagtccgcgt cccgtctgac ttcagccttt cgggtgttga ggggtcccca atgccgccgc
      841 atgacgcgaa gcccgagggc cgcatattcg accgcgaaag cggggcaggc cttggtcagc
      901 cgctggaatt ccttgccctc gccggtgccc caatcccgcc aggaactggc cccgcaggtg
      961 acgccctcgc cgaatatgtc gctgaagtcg gtcgagccct gataggcctg gaacaaggac
     1021 ctgaggcccg gatgggcgtc gatcgaattg aagctgacct ggaacagtcc cgcctcggcc
     1081 gtcgccgccg aaacgttgtt cgcggacatg tcgcgacctt cgcaatgctg gccggagctt
     1141 tcgcgcatcc ccagtcccat cagaagcgcg aagacatggc gaaggacgtc ggcgcccgcg
     1201 gtcgaattgc tcatggccag ggacgagaag gtggccgagt accaggcgag ggcgtcggtc
     1261 tggccgtcct gcgacgccgc cgccgcaatc cggcgggcaa tcggatcgcc ccgcttccat
     1321 ttgcaatagg tgcgcccgaa ggcgacggcc atgcccttcg tatagcctct gggcgcgcgt
     1381 ccgcgatcga gccaatgata ggttcggatg gccgaggtcg cggccagcgc ggagatgcgc
     1441 tgcgcgggcg aggactgcgc gtggagctcc gagcctcggc cccaatagat catcaagccc
     1501 gcgccgcccg cggcggccag tatctctcgt cggctgtagc tcatcctagg tctcccgatt
     1561 tcgccgagga tggccgatcc tcgaggcgtt cgtacgtgac gccgatcacg gcacgggaat
     1621 gtcgtcggcg ccgcctcacg ccttgaccgc ccggctccgg cggtgcttac gcctgccgcc
     1681 atgaccgaca tcgcctcacg cctcgccgag ctcggaatcg agcttccggc gcccgccgcg
     1741 cccgtcgcct cctacgttcc gaccgtgctg gccggcgacc tgctccacat ctccggccag
     1801 gtctcgttcg agaaggggga gctcctgacc ggccggctgg gcgaggaccg cgacctcgag
     1861 ttcgggatcc gcgccgcccg cgcctgcggc ctcatgctcc tggcgcagat cgagaaggcg
     1921 gtgggcctcg gccgggtgga gcggatcgtc aagctcggcg tgttcgtcaa cagcgccggt
     1981 tccttcaccg accagcccaa ggtcgccaac ggcgcctccg agctgatgca ggacctgttc
     2041 ggcgaggccg gccgccacgc ccgcagcgcg gtcggggtgc cggtgctgcc gctcggggcc
     2101 gcggtcgaga tcgatgcgat cgtccagatc aaggcgctct gagctcgagc ggctcggcgc
     2161 cgttcccttc gcccatcgcg ggctccacgg cgccggcctg gtcgagaaca gcggcgccgc
     2221 gatccgcgcc gccctggccg gcggatatgg gatcgagctc gacgtccagc tcagcgccga
     2281 cggcgaggcg atggtcttcc acgattacga gctcgaccgg ctgaccggcg accgtggcct
     2341 tctgcttcgc cggacggccg ccgagctggc cgccatccgc ttcgccgccg ccgacgaggc
     2401 gatgccgagg cttgccgagg cccttcgcat cgtctcggga cgcgggccgc tgctgatcga
     2461 gctcaagtcg cgcgaacgca gggtggaacg gctgtgccgg gccgtcgccg acgcgctcgc
     2521 cggctatgag ggtccggtcg gggcgatgtc gttcaacccg caggtcggcg cctggttcgc
     2581 tcgcaacgcc ccgcaggtgc tgcgcggcct cgtcgtcacc gagcagggca agaagggcct
     2641 gcgcggccgg atcgagcgcc ggctgttcct gtggcgggcg cgccccgact tcctcgccta
     2701 tgacatacgc gacctgccgt cgcgcttcgc cggccgggcc cgggcgtgcg gcctccccgt
     2761 ctacgcctgg actgtgcgga ccggcgccga ccgcgcgcgc gccgccgccc acgccgacca
     2821 gatcatcttc gaggcggcgc ccggatgagc gagatggtgg cccgcctggg ccaaggggtc
     2881 gtctcgttcg agccggccga ctgggaccgt tgcgcgggga ccggcgaccc cttcctaagc
     2941 cacgccttcc tgtccgcgct cgaggaatcg ggcagcgcga cggcgcggac cggctggcag
     3001 agcgtcccga tcgcgatcga cggcgcggac ggaatccccg ccgcggtgat gcccgcctat
     3061 gtgaagagcc acagccaggg cgaatatgtg ttcgaccatg gctgggccga cgcctgggag
     3121 cgcgccggcg gcgactatta tcccaagctg cagatcgcgg tgccgttcac cccggtcccg
     3181 ggccggcgcc tgctcgcccg gcgcgaggag ctcgcgcccg gcctgatcgc cgcggcggag
     3241 gcggtggtga ggcagcaccg cctctcctcg gcccatgcga ccttcatcgc cgagcgggag
     3301 gtgcctctgt tcgagcgggc ggggtggctg atcaggatcg acagccagtt ccactggcac
     3361 aaccgcggct acggcggctt cgaggatttc ctcgccgacc tctcgtcgcg caagcgcaag
     3421 gcgcttcgcc gcgaacgggc ggcggcgctc gccgggctga cggtcgagca cgtcaccggc
     3481 gccgcgatcg ccgaggcgca ttgggactcc ttctgggcct tctaccagga caccggctcg
     3541 cgcaaatggg ggcggcccta tctcacccgc aaattcttct ccctgctcgg cgagcggatg
     3601 gcggaccgga tcctgctcat cttcgcgctc cgggacgggc ggccgatcgc cggagcgctc
     3661 aacctgatcg gcgaggaggc gctctacggc cgctactggg ggacgatcga ggaggttccc
     3721 aacctccatt tcgagatctg ctaccaccag gcgatcgagg cggcgatcgg acgcggcctt
     3781 tcgcgggtcg aggccggggc gcaggggccg cacaagctga gccgcggcta tgcgccggtg
     3841 ccgaccttct cggcccattt catcccggac cccgatttcc gccgcgccgt cgccgacttc
     3901 ctcgccgccg agcgccgggc ggtcgcccgc gagatcgagg cgcttgccgg gatgaccccg
     3961 ttccgcaagg gatagggcga ccgattcgct gttccacccc ctctccctgc agggaagagg
     4021 gaggggcccg cgaagcggga gggtgagggc ctctcgacgt cgggaaagcc ctcacctccc
     4081 aagccttcgg cttgggcccc tcctctcccc ctacagggag agggtcatgt gaggagcgac
     4141 catgatccgc aaagccttcg ccgtcctgct gctcctcgcc gctccggccg ccgccgccga
     4201 cgggccgtcg cgcatcctcg aagcgcgcga cctgttctcg ctcgaggcgg cgagcgaccc
     4261 ccagatcagc cccgacgggt cgaagatcgc ctatgtccgg cgctccggcg acatcatgac
     4321 ggacaggatg cggccgacga tctggctggt cgacgcgcgc agcggcgagc aggtgccgct
     4381 cgccgccggg cccggctcgc acagccagcc gcgctggtct ccggacgggc gccggctcgc
     4441 ctacatctcg accgccgagg gcggcgcggc gcaattgttc gtgcgctgga tgggcagcgg
     4501 cgaatcggtc cggatcaccg gccttcccga cacgccgacg agcatcgcct ggtcgcccga
     4561 cggccgccag atcgcctaca cgatgttcgt tccgggagac gggctcaagc tcggcgccct
     4621 gccgccgaag cccgaggggg cgcaatgggc gccgccgctc gagatccaca gcgacgtcac
     4681 ctaccgcacc gacgaggagg gctatctgaa gcccggcttc agccacgtct tcctcgtcgc
     4741 cgcagacggc ggcgcgccgc ggcaattgag cttcggctcg gtccacgaca acggcccgct
     4801 gagctggacg cccgacggcc gcaccctttt gttcagcagc aaccgctcgc ccaactgggc
     4861 ccgggagccc ctcaacaccg aagtctatgc gctcgacctc gccagcggcg cgatttcggc
     4921 cctgacccgc cgcaacggcc ccgacgccga gccggtcgcc tcgcccgacg gccggctgat
     4981 cgcctatacc ggcttcgacg acaaggagcg cagctacgag aacgcgctgc tgtacgtgat
     5041 ggagcgcgac ggcggcagcc cgcgggcgct caccgcctcg ctcgaccgga gcgtcgactc
     5101 gccgcgctgg gccgccgacg gtcgttccat ctacgtcgcc tatgaggacg gggggacccg
     5161 caaggtggcc cgggtcgggc tcgacggctc gatccggacg gtggccgaag gcctcagcgg
     5221 agcgagcctg gatcgaccct ataccggggg cagcttcacc gtcgcgaggg acggcgcggt
     5281 cgccgctccg agcggaagcg cccagcgccc gcccgacgtc gccttgtatc gtgccggcgc
     5341 gaagcggcag ctgacccgac tcaacgccga actgctcgag cccaagcggc tgggcgaggt
     5401 ccgccacctg cgcgccgtct cgttcgacaa aaggccgatc gacgcctggc tcacgctgcc
     5461 gccgggccga gcggaggggc agaaggtgcc cctgatcctt gagatccacg gcgggccgtt
     5521 cgccgcctac gggccccatt tctcgacgga caaccagctc tacgccgccg ccggctatgc
     5581 cgtcctctcg gtaaatccgc ggggctcgac ctcctatggg gcggaattcg ccaacctcat
     5641 ccaccacgcc tatcccggcc gcgactatga cgatctgatg gccgcggtcg atgaggcgat
     5701 cgcgggcggc tacgcggatc cggacaagct gttcgtcacc ggcggctcgg gcggcggcgt
     5761 tctcaccgcc tggatcgtcg gcaagaccga ccggttcaag gccgcggcga cccagaagcc
     5821 ggtgatcgac tggaccagct tcgcgctcac cgccgacagc ccggccttct tctcgcgcta
     5881 ctggttcggc aaatatccgt gggaggatca cgaatccttc tggatccgat cgccgctgtc
     5941 gctggtgggg aacgtcagga ctccgacgct ggtggtggtc ggcagccagg attatcgcac
     6001 accggtcagc gaggccgagc aatattatac ggcgcttcag cttcggggcg tgccgacggc
     6061 gctggtcaag gtgccgggcg ctagccacgg cggcatcgcg gcgcggccgt cgcaggcggc
     6121 ggccaaggcg gcggcgatat tggcctggtt cgagaaatat aaggacgggg cgccggcgaa
     6181 gtgaattccc ctccctgcgt gcaggggggg agctacgccg cccgccgcac catcgtgtcc
     6241 gacagccagg cccgcgcctg gcgctgggct tcggcgatct cgcgggcggt catctcctcg
     6301 gcgatctcgg cgcggcattc ctggccgcgc tcgctgccgt tcagggcggc gaggttgaac
     6361 catttatgcg cttcgatgag gtcgacctcg aggccgtggg tgccggtgga gtaggcgacg
     6421 ccgagttcgt agagcgcgtc cggatcgccc atggcggcgt cggcgatgcg gctttcgatc
     6481 aggaaagtcg ctgacttcag gctgttcccc atttgacatg cccctatgtt ctatcgcgtg
     6541 tcggggacag gttgcgcagg cgatcaccaa caaatggtta acgccggagt caccatggaa
     6601 ttcctccccg cgatttcgcg gggaggggga ccgcgcgaag cgcggtggag gggttcttcg
     6661 gacgccgtaa gggcccctcc accatgcttc gcatggtccc cctcccctgc aaaagcaggg
     6721 gaggattact gcggctccac cgccagattg tcgatgaggc gggtccgccc gattctcgcc
     6781 gccgccagca gccgcgccgg cctgtcgacc ctggtcaccg gatcgagagt ctcggcgtcg
     6841 cacagctcga cataatcgat cgggtcgaag cccgctttgg tcagccgctc cttcgcggtt
     6901 gccagcgcct cagcaaccga gccgccgcgc tggatcgacg ccgccgcctc gccgagcgcg
     6961 cgcggcaggg cgcgcgcggc ctggcgctcc tcctcggtca gatacgcatt gcgcgacgac
     7021 agcgcgaggc cgtcggcgtc gcgctgggtg gggaccgcga cgatctcgac ggcgaggccg
     7081 cggtcccgga ccatctgccg gatcacggcg agctgctgat aatccttctc gccgaacagg
     7141 gcgatgtcgg gacggacctg ttcgaacagc ctcgacacca ccgtcgcgac gccgtcgaaa
     7201 tggccggggc gggcggcgcc gtcgagaccc tcgccgaggc tgccggcgcg aacgtcggtc
     7261 tcgaatccgt ccgggtacat ggtcgcggca tccggcgccc acaggatcgc gcagccttcc
     7321 tcctccagca tcttgccgtc ggtcgcctcc cggcgcggat aggtcgacag gtcctcattc
     7381 gccgcgaact gggtcgggtt gacgaagatc gaggcgacga catggtcggc ccgccgccgc
     7441 ccctcggcga ccagcgccat atggccggcg tggagggcgc ccatggtcgg cacgaaggcg
     7501 atcgtggcac cctgcgcgcg caattccgcc accgccgcgc gcagcgtttc gatgtcacgg
     7561 accgtttgca ccctcttgcc gcctccgata tcagcttggg cgacgccctt gcggggggcc
     7621 gagggcagaa tcaaggggtg cagatggcgt cgccgcattt catcgttttc gccaatgaga
     7681 agggcggcac cggcaagtcg acgacggccg tgcacagcgc cgtcgcgctc gccgcggccg
     7741 gccggcgggt ggcggcggtc gacctcgaca cccgccagcg cacgctcggc cgctatctcg
     7801 acaatcgggc ggagacggtg aagcggctcg gcatcgatct cgtcatgccc gcctacgaga
     7861 cgttcgatcc ggacaaggga gagccgatcg aggcgcagct ggatcggatc gcggcggacg
     7921 tcgagatcgt cgtcgtcgac accccgggcc gcgacgatcc gcacgcgcgc tcggcgatga
     7981 tccgggcgga caccctggtc accccgatca acgacagctt cgtcgatctc gatctcatcg
     8041 gccaggtcga cgccgagacc tatcgggtcc gccgccccag cttctacgcc gagctggtct
     8101 ggaacagccg cacccagcgg gccaaggccc atggcgccag cgtcgactgg gtcgtgctca
     8161 ggaaccgaat gcagcatatc gaggcgcgca acatgcgccg ggtcggcgag gccctgaccg
     8221 agctgtcgcg gcgggtgggc ttccgggtca taccgggcct cggcgagcgg gtcatctacc
     8281 gggagctgtt cccgaagggc ctgaccctgc tcgacctgcc gcacctcggc gaggtcggaa
     8341 tcgcccatat cgccgcccgc caggagctgc gcgagatgat cgcgggcttc gccttgccgc
     8401 agccgggcga ggcggcgccg gctcgggcgg cggccctggc cggctagccc gatgggcctt
     8461 ctcatcctcc tcgccgccgc cggcctgctc tgggccttcg ccaccggccg gctcaagggc
     8521 ttcacctacg aggacggcat tgccgccgcc tccttcctgc tcgggctgcg gctgttcacg
     8581 accgggcgcc tcatcctcgg ctccgcgctg atggccggcg ccctgctctg ggcggcctgg
     8641 cgccgccagc ggccgtcgac caagccgatg ccggccgagg acgcgcggag gctgctcggg
     8701 gtcggcgaag gcgccacgct ccaggagata cgcgacgccc accggcggct gatcgcgaag
     8761 gtccacccgg acgccggcgg ctcggcggag ctcgcccacc gggtcaacgc cgcccgcgac
     8821 acgctgctcg ccgaaacaat gaaccgcaag ccgcctcgcg cgtcttgacg gcgaatcccc
     8881 ctttgccgga gccgcgaatg agccatagcc tgcaccctac gtccctgcgc gaatatgaca
     8941 tccgcgggat atacggggag tcgctggcgg aggaggatgc ccgggccgtc ggccgcacct
     9001 tcgggacctt ggtgcgccgc ggcggcggcg gccgggtggc cgtcggccgg gacggccgga
     9061 tgagctcgcc gggtctggaa tcggccctga tcgaaggcct gacggggagc ggagtcgatg
     9121 tcgtgcgcat cggcctcggc ccgacgccga tgctctactt cgccgaggcc gagcttccgg
     9181 tcgacggcgg gatcatgatc accggcagcc acaatccggg caattacaac ggcttcaaga
     9241 tggtgctggg ccatggcgcc ttcttcggaa ggcggatcca ggagctcggc ggcatggccg
     9301 cggcgggcga ctgggagcag ggctcgggct cggtccgcga cgaggacgtc atggaacgct
     9361 atgtcgagcg gctcctccag gggttcgacg gcggtccgtt ccggatcggc tgggattgcg
     9421 gcaacggcgc cgccgggcct gtcgtcgagc gcctggtgaa aaggctgccc ggcgagcatt
     9481 tcaccttgtt caccgaggtg gacggccgct tccccaatca tcatcccgat ccgaccgtcg
     9541 agtccaacct cgaggacctg aagcggctgg tgcgcgacga acgtctcgat ttcggagtgg
     9601 ccttcgacgg cgacgccgac cggctgggag cgatcgacgg ccagggtcgc gtggtctggg
     9661 gcgaccagct cctgtcgatc ctcgccgagc ccgtgcttcg cgaggtgccc ggggccacca
     9721 tcatcgccga cgtcaaggcg agccaggccc ttttcgaccg ggttgccgag ctgggcggca
     9781 cgccactgat gtggaagacc ggccacagcc tgatcaagtc gaagatgagg gacaccggcg
     9841 ccccgctcgc cggcgagatg agcggccaca tcttcttcaa gcatgagtgg tacggcttcg
     9901 acgacgccat ctacgccgcc atccgcctga tccgcgccgt cgcgcagctg ggaggctcgc
     9961 tcaccgccct gcgctcggcg atgccgccga tgatcaacac gcccgagatg cgcttccagg
    10021 tcgacgagag ccgcaagttc ccggtcgtcg aggaggttct cgagcgtctc tcggccagcg
    10081 gggcgcaggt cgaccggacc gacggggccc gggtcagcac ccccgatggc tggtggcttc
    10141 tgcgcgcctc caacacccag gacgtcctcg tcgcccgggc cgaggccaag gacgaggcgg
    10201 ggctcgaacg cctcgtcgcg cagatcgacg agcagctcgc cttgtccggc ctccagcggg
    10261 gcgagcaggt cgcgcactga tgccgaggct ctacccggag gcgctgctct tctgcgcggc
    10321 ctgggcggcc ctcgccattt acggcgggct cgagctgggc tggaaagccg gcgccgtgct
    10381 caccctcggc ctgttcatcc tgatcatgcc gaccagcgcc gtcatcctct cgcgcaccgg
    10441 aagcttcaag ctggagcggg gcgtgcggtg ggccctgctg acggtggcgg ccgccgtgct
    10501 tctatcggcg ctcaacaatc agggctgagc ccgactaatc atcctcgtcc tcgcggccga
    10561 cgaggttgag ctcgcgcgcc ttgatctgcc gcgtcatgca ccagtgcatg agggcgtcct
    10621 cgcggccgtg ggtgatccac acctcgcggg gccgcagctc ctcgatggtc ccggtcagct
    10681 cgtcccagtc ggcatggtcg gagacgatca gcggcagctc gacgttgcgc tggcgggcgc
    10741 gctggcggac ccgcatccag cccgaggcca tggccgtgat cggatcggga aggcgcctcg
    10801 accagcggtc gttgaggcag ccgggcggcg cgacgacgat cttgccccgc aattcctcct
    10861 tgcccgcgcc ggtggcgggg cgcaggtcgc cgaggtcgat gccgtggtcg cggtagaggt
    10921 cgcagagccg ctggagcgcc ccgtggatgt agatcggctc gcggtggccg cggcccctga
    10981 gctccatgat caggcgctgg gccttgccga gggcataggc gccgaccagg acgcaccggt
    11041 cggggtttcc gtgcagccgg ccgagcagcc ggtcgatctc gctcgcggtc gccgggtggc
    11101 ggaagacggg gaggccgaag gtcgcctcgg tgatgaacac gtcgcagggc accggctgga
    11161 agggcgggca ggtcgggtcg gcgcggcgct tgtagtcgcc cgagacgacc acgcgctctc
    11221 ccccatgctc cagcacgatc tgggcggacc cgaggacgtg cccggccggg acgaagctgg
    11281 cctcgacgtc gccgaggcgc accacctcgc catagggggt cgcccggccg ccggcctgcg
    11341 gcccgtagcg gcattccatg atcgccagcg tctcgggggt cgcgaacacc tcgccgtggc
    11401 cgccgcgggc atggtcggca tggccgtggg tcaccagcgc gcgggccttg ggcaggttcg
    11461 gatcgaccca ggcctcggcc ggccttatgt acaagccttc gggaaaaggc tcgatccagg
    11521 aaccgagacg ggccatcggg agcttatatg gcggcgacgc ccccggccgc caaggcgggg
    11581 aggaaaggaa ggagcgcaac tgatggatct ggctggaggc caatgggcga ttctcaacat
    11641 cgtcgggccg ctgctgctcg ccgccgtcct gctttgggcg ctgcttcgca accggcgctc
    11701 gcgcaggaac ggttcgggca ccaccgagca ggccacccgc gacctctatc gcgaggagga
    11761 ggagaagcgg cgcagcgggg atgacgggcg cagctgagcc gctgctgccg gccgccgtcg
    11821 acgcctggtt cgcggccaag ggatggcagc cgcgaaggca ccagctcgag atgctcgacg
    11881 ccgcccggac cggccgcagc gccctgctgg tggcgccgac cggggcgggc aagaccctgg
    11941 ccggcttcct tccctcgatc gtcgagctga tcgaggcccc gaccgacggg ctccacaccc
    12001 tctacatctc gccgttgaag gcgctcgccg tcgacgtgca gcgcaacctc ctcaccccga
    12061 tcggggagat ggaccttccg atccgggtcg agacccgcac cggcgacacg cccgccgacc
    12121 ggaaggcccg ccagcgcgcc cggccgccgc aaatgctgct cacgacgccc gaatcgctga
    12181 gcctgctgct cagccatgag gacagcgccc tgctcttcga gactctcgcc accgtcgtgg
    12241 tcgacgagat ccatgccttc gcgaccggca agcggggcga cctcctgtcg ctcgccctct
    12301 cccggctgca gcgcctgtcc ccacggctgc gccgggtcgg cctgtccgcc accgtgtccg
    12361 acccggaggc cttccgaggc tggctcgccc ctcacggcga cgccgacatg gtcgagctgg
    12421 tcgagggcga gcccggcgct ccggcgcagc tcgccatcat gctgccggag gaggacaaga
    12481 tcccgtgggc ggggcattcc ggccgctggg ccgcgctcag cgtcatgcgc gagatcgagc
    12541 ggcacaagac gaccctgctc ttctgcaaca cgcgctcgct cgccgagctc atcttccagg
    12601 acctctgggc ggtcaacgag catcacctgc cgatcggcat ccaccacgga tcgctcgcgc
    12661 tcgaggcgcg ccggaaggtc gagaacgccg tcgccgaagg ccggcttcgc gcgctggtct
    12721 gcaccgccag cctcgacctc ggggtcgact ggggagacgt cgacctcgtc atccagatgg
    12781 gggcgccgaa gggctcgtcg cgtctcatgc agcggatcgg ccgggccaat caccggctcg
    12841 acgagccttc ggaggcgatc atcgtccccg gcaaccgctt cgaatatctg gaggcccgcg
    12901 ccgccctgga cgcgatcgag gagggcgcgc tcgacgccga gaacttccgt cccggggcgc
    12961 tcgacgtcct cgcccagcac atcatggcgc tcgcctgcgc ggcgccgttc cgcgaggagg
    13021 agatgctgga ggaggtgcgc tcggcggccc cttacgcggg gctgagggag gagacgttcc
    13081 tcgccatcct ctccttcatc gagagcggcg gctatgcgct cagggcctat gacaagttcc
    13141 gccgcatcgc cagggattcg gacggcttgt ggcgggtgtc gcatccgcgc ttcatccagc
    13201 agcaccgtat gaatgccggc atcatcgtcg acacgccgct ctactacgtg cgcttcagga
    13261 acggccgggt gcttgggacg gtcgaggaca atttcgccga gaccttgagc ccgggcgaca
    13321 ccttcttctt ctccggcctg gtgctcgagg tcgagcggat cgacggcatc gacctcatcg
    13381 tccacgccac gtcgaagtcg gcgcgaatcc ccacctacat gggcgcccgg atcgcgatca
    13441 gcaccaacct cgccgaccgc gtgcgcggct tcctccacga ccgccgtcaa tgggagcgct
    13501 ttcccgatcc ggtgcgcgac tggctgaggg tccaggaata tcgctcggcg atacccgcgc
    13561 ccgaccagct gctggtcgag accttcccgc acgagcgcat ccactacatg gccgcctaca
    13621 gcttcgaggg ctggaacgcg caccagtcgc tgggaatgct gatcacccgc cggatggaga
    13681 gctcggggct caagccgctc ggcttcgtcg ccaacgacta tgcgatcgcc tgctacgggc
    13741 tcgagccgat cctcgatccc gccccgctct tctcggccga catcctcgag aaggagttcg
    13801 tcgactgggt cgaaggctcg cacctcctga agcgcgcctt ccgcgaggtg gcggtgatcg
    13861 gcggcctggt cgagcgccac catcccggca ggcgcaagac cggcaagcag gtcaccttct
    13921 cgaccgacct catctacgac gtcctgcgcc gctacgagcc cgaccatctg ctcctgaagg
    13981 ccgcctgggc cgacgcccgc gagaagatga ccgacgtcgg ccgcctcgcc cggctgctcg
    14041 accgcgccca gcgcacgatg ctccacgtcg agctcgaccg ggtctcgccg atggcggtcc
    14101 cggtgctcac catcatcggc cgcgaacgca tgccccaggg cacggtcgac gaccagcttc
    14161 tgatcgaggc cgagagcctg gccgcgacgg cgatgcgcat cgattaggtc gcggaaattc
    14221 ctccccgcga tttcgcgggg agggggacca ggcgaagcct ggtggagggg ttctgccagc
    14281 gcccgaagaa cccctccacc gcgcttcgca tggtccccct ccccggcaaa tgccggggga
    14341 ggaattttca gccctgcagc cgcgcccgga accggcggct gccggcgagg cgctcgccgc
    14401 tctccatcac cagctcgaaa tcgccgctcg ccttcgtctc gatctccttc accgcggcgg
    14461 cccggacgag ccgcgagcgg tggatccgcc ggaagccgtg cggagccagc agcgcctcga
    14521 ggctcgccag cgtctcgcgg tggaggcgct cgccgaagcg gccgtgcagc tcgacataat
    14581 tgccggcggc ctgggcccat tcgatgtcct ccggcgcgag ccacgcggtg cggccgccgt
    14641 cgcgcacctc gagccggaag cccccgccgg gcgccgccgc cggcgccggc ggagcgagcc
    14701 ggcgcgcgag cgcgaacagc agggcgagca ggaggtaggt gacgaggtcc ttgcgatatt
    14761 cgtagaccag cacgcccggc accgagccgg cgaatcgata ggttccgtcc agggccgcgt
    14821 agatcgccat ccgcgccgtc atcatgaagc cgacatgggc cagcgacacg gcgaccgaga
    14881 gcaaggcatg aagggccagc gccgccggcc agcgaaagcg cggcggtcga agctgcgcag
    14941 acgctgcgaa gacgaccggg gccagcgcaa gccaggcgcc caggctggtc agctcccagg
    15001 tccagggctg ccaggccggg accggctggc cgaggcggaa ccggtcgtcg acgacgctgg
    15061 tcgcgttgac cgcggcgttg atgccgccga tcgccgcgaa cagcagccag ggaagccacc
    15121 agccctgacg ctccgcgccg ctcgtcccgc ctggatcgcc gctcgtcacc ggacccttcc
    15181 cgccgtcccc tccgcgccgc cggtcgtcac cccggcgccg cctgccgcac cgcaccgcgc
    15241 tacacccggt ccggcaactg acagcaaggc aggaagcgat gcaagacagg ggttcgggag
    15301 agcggcggtt cgggctggac tggctgagga tcggggcctt cgccctgctc atcctctacc
    15361 atatcggaat gttctttgtg ccgtgggact ggcatgtgaa gaccagccgc ccgcaggagt
    15421 ggctggagct gccgatgctg gctttgaacc cctggcggct cgccttgctc ttcgtcgtct
    15481 cgggcgtcgc ctcgcgggtc ctcctggcga ggatggccgg gccgggcgcc tttgccgccc
    15541 tgcgctcccg gcggctgttg attccgctgg cggcgggcat ggccctcttc gtggcgcccc
    15601 agccctgggc gcagcttcag gagcaggccg gctacgccca gggcttctgg catttctgga
    15661 cccgggacta tttcgagttc ggcggcagcc tcggcctcat cctgccgacc tggaaccacc
    15721 tctggttcgt cgcctatctg tggctctaca cgatggcgct cgcccttctg gccggcttgc
    15781 cgccgacggc gcgggcgtcg gttcagcggg ccttcgagcg cctgttcgag ggccggcggc
    15841 tgctctggct gccgatcctg tacttctggc ttctgcgcat cggcctttac ccggccttcg
    15901 gcgaaactca cgccttgctc gacgatcctt atgcccatgc cttctacggc gccgccttct
    15961 tcttcggagt cggcctggcc cgcgccgacg gcctgtggct gcgcctggtc gccggctggc
    16021 agccggcggc ctggctggcg ctggccggct acggggcgat cgtcctcctc gacctcacca
    16081 tcccgggcga atcggggccg gctgagctcc tggccgcccg gatggcgcgg tcggtccagg
    16141 cctgggcgac gatcctcgcc ctgctcggct tcgcccagcg ccacctccat cgcgacggac
    16201 cggcgcggcg ctacctgacc gaggcgatct ttccctggta catcgcgcac cagacgatca
    16261 tcgtcctcgc cggccactgg ctgcggccgc tcggcctcgg ccccggcgtg gagttcgcga
    16321 tccttctcgc cgcgactctg gcgggctgcg ccgccaccta cgagatcggc cggcggatcg
    16381 cctggctccg ccccctgctc ggcctccgtc cggggcaacg cgctgccgcc ccggcaccat
    16441 cggcggcggc cgcggctccc tgacgtcgcc cccttcccct gcaattcctc ccctgcattt
    16501 gcaggggagg gggaccaggc aaagcctggt ggaggggacc ttgccgagtc cgaagaaccc
    16561 ctccaccgcg cttcgcgcgg tc
//
LOCUS       DASVQZ010000261         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_154082, whole genome shotgun sequence.
ACCESSION   DASVQZ010000261 DASVQZ010000000
VERSION     DASVQZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154082"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VF547_07185"
     CDS             complement(<1..733)
                     /locus_tag="VF547_07185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8262640.1"
                     /translation="MERVVYDRMAEYADRHWWYRARRDILATLIEQEIALPENARLLE
                     IGCGTGPNLDMLGRFGRLDAVEIDARARAIASERLGRRVLDAPLPHLHGVEDGAYDLV
                     CLLDVLEHVEADREALVSIARKLRPGGRILIAVPAHPWMWSAHDRVNHHKRRYTKKTL
                     RAVIDAAGLRLHLMSYFNSLLFPLAVTQRLAAKMLHKGEDDSADTPPPAALNRLFETA
                     FRLERYAIGRLPFPPGLSIVAIVSAS"
     gene            complement(733..1137)
                     /locus_tag="VF547_07190"
     CDS             complement(733..1137)
                     /locus_tag="VF547_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8262641.1"
                     /translation="MRDAAALRTLAGQMVRFGLVGLFSTALYAVIYWPLATYVMPPVL
                     AVVVAFIIAALIGYYLHSRWSFKGHGKEEGSPATQGKFFTVQGVGMLLNMAFTWVLTG
                     PLFHGPTWWPLVPAVLVTPFVTFGLNRWWVFG"
     gene            1221..2987
                     /locus_tag="VF547_07195"
     CDS             1221..2987
                     /locus_tag="VF547_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HEX8262642.1"
                     /translation="MEGLDRLEKHWRWWLLLFWVAAAAGLIFSKWAAIRQFGLPDTDD
                     NIRIMEVRAWLGGQSWYDLRQYRLNPPSGADVHWSRLVDIPIAGLKLALTPFLGGASA
                     EKAAVTIAPLLPLGVAMAAVATIARRLIAPSAFALAIAVLLCAHSPRAMWTPLRIDHH
                     GWQLAFLAWAVAGLIDPKRARGGAVLGLATALSVAIGLEMLLYLATLGAIAVVLWIRD
                     PAEGRRLITYGGTLAGGTGLAYLLFASWANRAPVCDALSPVWLSGLVGAGAVAALLAL
                     LPLRSVPLRFAAAAAAGVVVAAGFALAWPHCLGRLENVSPEATRLWLDNVREARPIYM
                     HGTQTMLIVCALPVAGLVGYAAMLWRSRREPEVLVRWAAVALPALLAAALLLWQARAG
                     PAAQLLAVPGATGLAWLLIPKLMGSRMMLVRVLGTVAAFMLVSGLLPQQLAQYIPQKP
                     RPVLKTVNRANGLCWSLWGLHPVALVPKGQVLTHVDLGPRLIAVTHHDAVAGPYHRNG
                     KDIVDVMMAFRGSADFAREVVARRKIDYVLVCPGMSETTIYASQAKNGFYVQLMKGQV
                     PAWLETVPLPKDSPFRMWRVRR"
BASE COUNT          429 a         1038 c         1073 g          482 t
ORIGIN      
        1 acgaagcgga gacgatggcg acgatcgaga gcccgggcgg gaacggcagc cggccgatcg
       61 cgtagcgctc caggcggaag gcggtctcga acaagcggtt gagcgccgcc ggcggcggcg
      121 tgtcggcgct gtcgtcctcg cccttgtgca gcatcttcgc cgcgaggcgc tgggtgacgg
      181 cgagcgggaa gagcagggaa ttgaagtagc tcatcaggtg gagccggagg cccgcggcgt
      241 cgatgacggc gcgcagcgtc ttcttcgtgt agcggcgctt gtggtggttc acccggtcgt
      301 gggcgctcca catccaggga tgggcgggga cggcgatgag aatgcgcccc cccggccgaa
      361 gcttgcgggc gatgctgacc agcgcctccc ggtccgcctc gacatgctcg aggacgtcga
      421 gcaggcagac caggtcgtag gcgccatcct cgacgccgtg gaggtggggg agcggcgcgt
      481 cgagcacccg gcggccgagc cgttcgctgg cgatggcgcg ggcgcgggcg tcgatctcga
      541 ccgcgtcgag ccggccgaag cggcccagca tgtcgagatt gggcccggtg ccgcagccga
      601 tctcgagcag gcgagcattc tccggcaggg cgatctcctg ctcgatcaag gtcgccaata
      661 tgtcgcgccg cgcgcggtac caccagtggc ggtcggcata ttcggccatg cggtcgtaga
      721 cgacgcgttc catcagccga acacccacca gcgattgagc ccgaaagtga cgaacggggt
      781 gacgaggacc gccggcacca gcggccacca ggtcgggccg tggaagagcg gcccggtcag
      841 cacccaggtg aaagccatgt tgagcagcat gccgacgccc tgcaccgtga agaacttgcc
      901 ctgcgtcgcc ggcgagcctt cttccttgcc gtggcctttg aagctccacc ggctgtgcag
      961 gtaatagccg atgagcgcgg cgatgatgaa ggcgacgacg acggcgagca ccggcggcat
     1021 cacgtaggtg gcgagcggcc agtagatcac ggcgtagagc gcggtcgaga acaggccgac
     1081 cagcccgaag cggaccatct gtccggccag cgtcctgagc gcggctgcat ccctcatctc
     1141 agcgcgttct ccagccgggg ccgcttgccc tttgccgcca gcggactaat ccggaccccg
     1201 cgggttggaa aggggaaagc atggaggggc tcgatcgcct ggagaagcat tggcgctggt
     1261 ggctgctgct tttctgggtc gccgccgccg ccgggctgat cttctccaaa tgggcggcga
     1321 tccgccagtt cggcctgccc gacaccgacg acaatatccg catcatggag gtccgcgcct
     1381 ggctgggggg gcagagctgg tacgatctga ggcagtaccg gctgaacccg ccctccggcg
     1441 ccgacgtcca ctggtcgcgg ctggtcgaca tcccgattgc cgggctgaag ctcgccctca
     1501 cccccttcct cggcggagcg tcggcggaga aagcggcggt aacgatcgcg ccgctcttgc
     1561 cgctcggcgt cgccatggcc gccgtggcga cgatcgcccg ccgcctgatc gcgccgtccg
     1621 ccttcgcgct cgcaatcgcc gtgctgctgt gcgcccactc cccccgagcg atgtggacgc
     1681 cgctccgcat cgaccatcac ggctggcagc tcgcctttct cgcctgggcg gtcgcggggc
     1741 tgatcgaccc gaagcgggcg cgcggcggcg ccgtgctcgg cctcgccacc gctctgtcgg
     1801 tcgcgatcgg gctggagatg ctgctctatc tggcgacgct cggcgcgatc gcggtcgtgc
     1861 tgtggatccg cgatccggcg gaaggacggc ggctcatcac ctatggcggc acgctcgcgg
     1921 gagggacggg gctcgcctac ctcctcttcg cctcctgggc caaccgcgcc cctgtctgcg
     1981 acgcgctgtc gccggtctgg ctctcgggac tcgtcggcgc gggagcggtg gcggctttac
     2041 tggcgcttct gccgctgcgc tcggtcccgc tccgcttcgc cgcggcggcc gcggcgggcg
     2101 tggtggtcgc cgccggcttc gcgctcgcct ggccgcactg cctcggccgg ctcgagaacg
     2161 tctccccgga agcgactcgc ctgtggctcg acaatgtccg ggaggcgcga cccatctaca
     2221 tgcatggaac gcagacgatg ctgatcgtgt gcgcgctgcc ggtcgcgggg ctggtcggct
     2281 atgccgcgat gctgtggcgc agccggcgtg agcctgaggt cctggtccgc tgggccgcgg
     2341 tcgcccttcc cgccctgctc gccgccgccc tcctcctctg gcaggcccgc gccggacccg
     2401 cggcccagct cctcgccgtc cccggcgcga ccgggctcgc ctggctgctc atcccgaagc
     2461 tgatggggag ccggatgatg ctggtgcgcg tgctcggcac cgtcgccgcc ttcatgctcg
     2521 tctccggcct gctcccccag caattagcgc aatatatccc gcaaaagccg cggccggtgc
     2581 tgaagacggt gaaccgcgcc aacggcctgt gctggagcct gtggggcctc catccggtgg
     2641 cgctggtgcc caagggacag gtgctgactc atgtcgacct cggtccgcgc ctcatcgccg
     2701 tcacgcacca cgacgccgtc gcggggccct atcatcgcaa cggcaaggac attgtcgatg
     2761 tgatgatggc cttccgcggc agcgccgatt tcgcacgaga ggtcgtggcg cggcgaaaga
     2821 tcgactacgt gctcgtctgc cccggcatgt cggagaccac catctatgcc agccaggcga
     2881 agaacggctt ctacgtgcag ctgatgaagg ggcaggtgcc ggcgtggctg gagaccgtgc
     2941 cgctcccgaa ggattcgccg ttccggatgt ggcgggtcag acgctgaagc tgacggagat
     3001 gccgtcgatc acgaactggg cg
//
LOCUS       DASVQZ010000262        11331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_729185, whole genome shotgun sequence.
ACCESSION   DASVQZ010000262 DASVQZ010000000
VERSION     DASVQZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729185"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="VF547_07200"
     CDS             <1..2004
                     /locus_tag="VF547_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8262643.1"
                     /translation="VGTRTIALVGPGGSGKTSLAEALLFAAGAIDRQGSVDAGTSVGD
                     SSPEARQRAGSTELNLMHFDFLGDRFAIVDSPGSVGFSADGALAVAAADLAVVVVDPD
                     TDRAILAEPALRQLEKLGVPHLIFVNKIDQARGGIRALLEALQPMSSSPLIARQIPIR
                     EGEKVTGFVDLALERAFKYRPGKPSEQIDIPPDLAEREKGDRFHMLEQLADHDDALLE
                     QLLMDETPDQATIFADIARETKENLVVPVLFGSALNGFGVRRLMKALRHDAPAPEAAA
                     ERLGAKAGCAFVFKVSHGGNMGRLALARVFGGTLSEGTELKAASGDTVRVGTLFAMQG
                     EKPAKLGRANVGDVVAIAKAEGVNAGEWLGAGGDIPKLDLPASNYGLAIATRDRKDDV
                     RLSTALHKLCEEDPSLGWEQDEALHETRLKGISDEHLKVALARLQRRYGVAVDAHPPV
                     VGYKESIRKPVTQRGRHKKQSGGHGQFGDVVIEIRPLGRGEGFKFEEKIHGGAVPRQY
                     IPAVEMGVRDALAKGPLGFPVVDVAVTLVDGSYHTVDSSELAFRTAGRIGMSEALAAA
                     QPHLLEPVHKVTVVTPGYATSRVTSAIASRRGQMLGMDSRAGWSRWDVVEALVPEAEL
                     QGLDAELRSVSQGLATYQAEFDHLAELNGKLADDVVQKVLEPA"
     gene            2166..3482
                     /locus_tag="VF547_07205"
     CDS             2166..3482
                     /locus_tag="VF547_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8262644.1"
                     /translation="MAKRLTYWILVGLVLGIFVGWGLNFLLGDGTTEGGPVVKEVARY
                     LSILTTIFLHLIKMIIAPLVFSTLVVGIAHMGDTAALGRVGVKALGWFVAASLVSLSL
                     GLLLVTLFQPGTGLDLALPPATASAGVDRSAFDIVKFFGNIFPESMIRAMSENSILQI
                     VVFSLFVGVAITAVGEPARPLVRATEALVKVMLVITDYVMRFAPIAVFAAVASTVAQR
                     GPAILGTFGYFMGSFYIGLILLWLLLLGICFVIVGQRTRLLVRYIRDPIVLAFSTASS
                     EAAYPRTLEALDRFGVPPRIASFVLPLGYSFNLDGSMMYMTFATMFVAQAYGIELSMT
                     QQILMLLTLMITSKGVAGVPRASLVVITATMAQFNIPEAGLLLILAVDHFLDMGRSAT
                     NVVGNAVASVVVAKWEGALDPLEPADIEPPHAPAHVPPPRPGDEPF"
     gene            complement(3630..4883)
                     /locus_tag="VF547_07210"
     CDS             complement(3630..4883)
                     /locus_tag="VF547_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX8262645.1"
                     /translation="MPELSRRDLALGALAAGALALRPPPLRAAPAPPLRPITADVDPI
                     GTAERLQRLEKARQLMRRHGLGSILVEPGASLVYFTGVQWWRSERLTAALIPVEGEPI
                     VFTPFFEEPSVRESLGIPAEIRTWNEHEDPLRLVAGALRDRKAAGRPVGIEETVRFFA
                     IDGLRKAMPRVDIRNAAPVVRGCRMVKSPHELRLMQKAADVTIAAYRHTWRRIEKGMT
                     PADIGAIMNGATEALGGTPQFALVLLGEASAYPHGSGKPQQVRDGEVVLMDCGCTVHG
                     YQSDISRSFVFGEPNAEQRKVWTQMRRGQDIAFAAARVGATAGSVDDAVRRQYEAWGY
                     GPGYRLPGTSHRTGHGIGLEGHEPVNFVHGETTRLAPGMCFSNEPGIYLPGKFGIRLE
                     DCLYITEAGPRWFSTPPPSIDRPFD"
     gene            complement(4876..5496)
                     /locus_tag="VF547_07215"
     CDS             complement(4876..5496)
                     /locus_tag="VF547_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8262646.1"
                     /translation="MILVHHLENSRSQRVLWLLEELGLPYEVRRYERNRKTMLAPPEL
                     RRVHPLGKSPVIEDEGIVVAETGAIVEYLVEKADGRLGPRRREEVLRYRHFLHYAEGS
                     LMPPLLVMLVVRRLGLLGRPARATVQRMIDQHLDWLEAELSNRPWFAGDSFTAADVMM
                     SFPLEAARQRAGLDDGRPALVDWLERIHARPAYGAALKKGGPYAYA"
     gene            complement(5493..6323)
                     /locus_tag="VF547_07220"
     CDS             complement(5493..6323)
                     /locus_tag="VF547_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX8262647.1"
                     /translation="MDTEGATASILELRLIGAPTDRHSSFLRGAAKAPPLIRDALGSD
                     HGNLTAESGVELGVDVFLADAGDLPLTEGEGDDALIAATVAEAAAAGAVPLVLGGDHS
                     VTVPVLEALAAAHGAIDILHFDAHPDLYDDFEGNPRSHASPFARIMERGLARRLVQVG
                     IRTLNRHCREQAERFGVEIVEMRHFSPDAVPLPTGPLYISLDLDGFDPAFAPGVSHHE
                     PGGLSVRDVLAVLHRVRAPIVGADVVEYNPVRDVNGVTAVLAAKLVKEVAALCHRNNR
                     "
     gene            6526..7752
                     /locus_tag="VF547_07225"
     CDS             6526..7752
                     /locus_tag="VF547_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX8262648.1"
                     /translation="MLQPPRPDLADARPGFREFVVLIAGLMSLNALAIDAMVPALPAI
                     GQALNVADDNSRQLVVSLYVLGFGLTQIIYGPLSDRFGRKPVLAVSLVLYVGFALACW
                     AAPSFTLLLAARMAQGGAAASTRVLVVSIVRDRFEGPRMARIMSLAFLVFLLIPMLAP
                     VFGQLTLRVASWRAIFFGLALGGAIMLTWTLVRLPETLRPEYRRPLDAGTIWDGTCQT
                     LSNRQSLGYTLAFTLMMASLMGYINSIQQIVFDIFHRPELIAATFAGIALPMAFTSWL
                     NSRIVERIGTRRVAHAGLALFTASALVHLAIVLTTGESLWLFIGLQGLTMACFGLASA
                     NFGALAMQPLGHVAGTASSVQGTIGTIGGALLGLAVGQSFDGTLVPMVGSFAAFGAFG
                     LVILLWTERGRLFGRG"
     gene            complement(8057..9121)
                     /gene="recA"
                     /locus_tag="VF547_07230"
     CDS             complement(8057..9121)
                     /gene="recA"
                     /locus_tag="VF547_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX8262649.1"
                     /translation="MAASLKVVGNSDNMDRQKALEAALAQIDRAFGKGSAMKLGSREA
                     VEIETISTGSLGLDIALGIGGLPRGRVIEVYGPESSGKTTLALHVIAEAQKNGGTAAF
                     VDAEHALDPVYARKLGVDVDELIVSQPDTGEQALEITDTLVRSNAIDVLVVDSVAALV
                     PRAEIEGEMGDSHVGLQARLMSQSLRKLTGSISKSKCLVIFINQLRMKIGVMYGNPET
                     TTGGNALKFYASVRLDIRRTGQIKDRDDIVGNTTRVKVVKNKVAPPFKQVEFDIMYGE
                     GISKVGEILDLGVKAGLVEKSGAWFSYDSIRIGQGRENAKNYLRENPDVAARLERAIR
                     GKTDEVAEALMAGPAAEDDV"
     gene            9386..10093
                     /locus_tag="VF547_07235"
     CDS             9386..10093
                     /locus_tag="VF547_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8262650.1"
                     /translation="MPRSARLTVPGLPHHVTHRGNRRQRTFFTEADHRLYVRYLAAAC
                     AATGTAVWAWCLMPNHVHLVLVPSAEGGLSAVLHRTQGRYTRAINAREKWDGQLWQGR
                     FASFLMDERHLLACARYVELNPVRAGLVARAADWPWSSARTHLGGAADPLTDPAPLLG
                     RWPDWASVLDSGDDARSLQAIREHERSGRPLGSKAFLERLERATGRTLRPRRRVRIAA
                     PRPACDDPPRAPGTVPP"
     gene            10811..>11331
                     /locus_tag="VF547_07240"
     CDS             10811..>11331
                     /locus_tag="VF547_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262651.1"
                     /translation="MPILSGSLRAFLAALAGLAALAVPAAAAGQTRGQAAKDPAQAIF
                     ERCRSPDFAKARDCYAAALDDRLATGDPAAALQLLDGLTALDPEVRRHGHFYAHRIGI
                     SALKSPAEVGRVFAACTPAYESGCYHGVIQSYFIATERDGGGVTTQSLDALCGDYRGK
                     RADLLFQCTHGLG"
BASE COUNT         1621 a         4026 c         3853 g         1831 t
ORIGIN      
        1 gtaggcacca ggacgatcgc cctcgtaggc ccgggcgggt cgggcaagac cagcctggcg
       61 gaggccctgc tcttcgcggc gggggcgatc gaccgccagg gcagcgtcga cgcgggcacg
      121 agcgtcggcg attcgagccc cgaggcgcgg cagcgcgccg gctcgaccga actcaacctg
      181 atgcatttcg atttcctcgg cgaccgcttc gcgatcgtcg attcgccggg gtcggtcggc
      241 ttctcggccg acggcgcgct ggccgtggcc gccgcggacc tcgccgtcgt cgtcgtcgat
      301 cccgacaccg accgcgccat tctcgccgag cccgcgctga ggcagctgga gaagctcgga
      361 gtcccccacc tcatcttcgt caacaagatc gaccaggccc gcggcggcat ccgcgcgctg
      421 ctcgaggcgt tgcagccgat gagctcgtcg ccgctcatcg cccgccagat cccgatccgc
      481 gagggcgaga aggtcaccgg cttcgtcgac ctcgccctcg agcgggcctt caagtaccgg
      541 ccgggcaagc cttccgagca gatcgacatc ccgcccgatc tcgccgagcg cgagaaaggc
      601 gaccgcttcc atatgctcga gcagctcgcc gaccatgacg acgccctgct cgagcaattg
      661 ctgatggacg agactcccga ccaggcgacg atcttcgcgg acatcgcccg cgagaccaag
      721 gagaacctgg tcgtgccggt gctgttcggc tcggcgctga acggcttcgg agtgcgacgc
      781 ctgatgaagg cccttcgcca cgacgccccg gcgccggagg cggcggccga gcggctcggc
      841 gcgaaggcgg gctgcgcctt cgtgttcaag gtctcgcacg gcggcaacat gggccggctc
      901 gcgctcgccc gggtgttcgg agggacgctg agcgagggca cggagctgaa ggcggcctcg
      961 ggcgacaccg tccgcgtcgg caccctgttc gcgatgcagg gcgagaagcc cgccaagctc
     1021 ggccgcgcca acgtcggcga cgtcgtcgcg atcgccaagg cggagggagt gaatgccggc
     1081 gaatggctgg gcgccggcgg cgacatcccc aagctcgacc tgcccgcctc caattacggg
     1141 ctcgccatcg ccacgcgcga ccgcaaggac gacgtccggc tgtccaccgc gctccacaaa
     1201 ttgtgcgagg aggatccgtc gctcggctgg gagcaggacg aagcgcttca cgagacccgc
     1261 ctcaagggca tcagcgacga gcatttgaag gttgcgctcg cgcgcctcca gcggcgctac
     1321 ggagtcgccg tcgacgctca cccgccggtc gtcggctaca aggaatcgat ccgcaagccg
     1381 gtgacccagc gcggccgcca caagaagcag tcgggcggcc acggccagtt cggcgacgtg
     1441 gtcatcgaga tccggccgct cggccgcggc gaaggcttca agttcgagga gaagatccac
     1501 ggcggcgccg tgcccaggca atatatcccc gcggtcgaga tgggagtccg cgacgccttg
     1561 gccaagggac cgctcggctt tccggtcgtc gacgtggcgg tgaccctggt cgacggcagc
     1621 taccatacgg tcgacagctc ggagctcgcc ttccggacgg cggggcggat cgggatgagc
     1681 gaggcgctcg ccgccgccca gccgcatctg ctcgagccgg tccacaaggt gacggtggtc
     1741 actccgggct acgccacctc gcgggtcacc tccgcgatcg ccagccggcg cggccagatg
     1801 ctcggcatgg acagccgcgc cggctggtcg cgctgggacg tggtcgaggc gctggtgccg
     1861 gaggccgagc tgcaggggct ggacgcggag ctgcgctcgg tcagccaggg cctcgccacc
     1921 taccaggccg agttcgacca tctcgccgag ctgaacggca agctcgccga cgacgtcgtc
     1981 cagaaggtgc tcgagccggc gtagctcagg ccgtcattgc gagcgcagcg aagcaatcca
     2041 gcgcgggttc ggaggcgcgc tggattgctt cgtcgcttcg ctcctcgcaa tgacgggccc
     2101 cctccccttg acccgcgccg cgctttcgac ttcctaggcg ccacctgcag cacgaggagc
     2161 cgagcgtggc caaacgtctc acctactgga tcctggtcgg tctcgtcctc ggcatcttcg
     2221 tcggctgggg cctcaacttc ctgctgggcg acggcacgac cgaaggcggg ccggtggtca
     2281 aggaggtggc gcgctatctc tccatcctga cgacgatctt cctccatctg atcaagatga
     2341 tcatcgcgcc gctggtcttc tcgaccctcg tcgtcggcat cgcccatatg ggcgacaccg
     2401 ccgcgctcgg ccgggtcgga gtgaaggcgc tcggctggtt cgtcgccgcg agcctcgtct
     2461 cgctctcgct cggcctgctg ctcgtcaccc tcttccagcc gggcaccggc ctcgacctgg
     2521 ccctgccgcc ggcgacggcg tcggccgggg tggaccgctc cgcgttcgac atcgtcaagt
     2581 tcttcggcaa catcttcccg gaatcgatga tccgggcgat gtcggagaac agcatcctcc
     2641 agatcgtcgt cttctcgctg ttcgtcggag tcgccatcac cgcggtcggc gagccggcgc
     2701 ggccgctggt ccgggcgacc gaggccctgg tcaaggtgat gctggtgatc accgactatg
     2761 tgatgcgctt cgccccgatc gcggtgttcg ccgcggtcgc ctcgacggtc gcccagcgcg
     2821 gccccgcgat cctcggcacg ttcggctatt tcatgggcag cttctatatc ggcctgatcc
     2881 tgctctggct cctgctgctc gggatctgct tcgtcatcgt cggccagcgc acccggctgc
     2941 tcgtccgcta catacgcgat ccgatcgtcc tggccttctc caccgcctcc tcggaagcgg
     3001 cctatccccg cacgctcgag gccctggacc ggttcggagt ccctccgcgc atcgccagct
     3061 tcgtcctgcc gctcggctat tcgttcaacc tcgacggctc gatgatgtac atgaccttcg
     3121 cgaccatgtt cgtcgcccag gcctatggca tcgagctgtc gatgacccag cagatcctga
     3181 tgctgctgac gctgatgatc acctccaagg gcgtcgccgg agtgccccgg gcgagcctcg
     3241 tcgtcatcac cgcgaccatg gcccagttca acatccccga ggccggcctg cttctgatcc
     3301 tcgccgtcga ccatttcctc gacatgggcc ggtcggcgac caacgtcgtc ggcaatgcgg
     3361 tggcgagcgt ggtcgtcgcc aagtgggaag gcgcgctcga cccgctcgag cccgccgaca
     3421 tcgagcctcc ccacgccccc gcccacgtcc cgccgcccag gcccggcgac gagcctttct
     3481 agtctagcga aaaatccttc ccggcttttg ccggggaggg ggaccaggcg aagcctggtg
     3541 gaggggttct tgccgacccc gaagaacccc tccaccgcgt tgcgcgcggt cccccctccc
     3601 cagtaaatcc cggggaggaa tttttgccgt cagtcgaagg gccggtcgat gctcggcggc
     3661 ggggtcgaga accagcgcgg tccggcctcg gtgatgtaga ggcagtcctc gaggcggatc
     3721 ccgaacttgc cgggaaggta gatgcccggc tcgttcgaga agcacatgcc cggcgccagc
     3781 ctcgtcgtct cgccatggac gaaattgacc ggctcgtggc cctccaggcc gatgccgtgg
     3841 ccggttcggt gcgaggtgcc gggcagcctg tagcccgggc cgtagcccca ggcctcgtac
     3901 tggcgccgga cggcgtcgtc gacgcttccc gcggtcgcgc cgacccgggc ggcggcaaag
     3961 gcgatgtcct ggccgcgccg catctgggtc cagaccttgc gctgctcggc gttcggctcg
     4021 ccgaacacga agctgcgcga gatgtccgac tggtagccgt ggaccgtgca gccgcagtcc
     4081 atcagcacca cctccccgtc ccgcacctgc tggggcttgc cgctgccgtg cggataggcg
     4141 ctcgcctcgc cgagcaggac cagcgcgaat tgcggcgtcc cgccgagcgc ctcggtggcg
     4201 ccgttcatga tcgctccgat gtcggccggg gtcatgccct tctcgatccg gcgccaggtg
     4261 tggcgatagg cggcgatggt gacgtcggcg gccttctgca tcagccggag ctcgtgcggc
     4321 gacttcacca tccggcagcc gcggacgacg ggggccgcgt tgcggatgtc gactcgcggc
     4381 atcgccttcc tgagcccgtc gatcgcgaag aagcgcaccg tctcctcgat cccgaccggg
     4441 cggcccgcgg ccttgcggtc cctgagcgcg ccggcgacga ggcgcagcgg atcctcatgc
     4501 tcgttccagg tgcggatctc ggcggggatg ccgaggctct cgcggaccga cggctcctcg
     4561 aagaaggggg tgaagacgat cggctcgccc tcgacgggga tgagcgcggc ggtgagccgc
     4621 tcgctccgcc accattggac gccggtgaaa tagaccagcg aggccccggg ctccacgaga
     4681 atcgagccga gaccgtgccg acgcatcagc tggcgcgcct tctcgagccg ctggagccgc
     4741 tcggcggtgc cgatcggatc gacgtcggcg gtgatcggcc gaagcggcgg ggccggggcg
     4801 gctcgaagcg gcggcggacg aagggcgagc gctccggcgg cgagcgcgcc gagcgccaag
     4861 tccctccggc tgagctcagg cataggcata gggtccccct ttcttgagcg ccgctccata
     4921 ggccggccgg gcgtggattc gctccagcca atcgaccaaa gcgggccggc cgtcgtcgag
     4981 gcccgcgcgc tggcgcgccg cctcgagcgg gaagctcatc atcacgtcgg cggcggtgaa
     5041 ggaatcgccg gcgaaccagg gccggttcga aagctcggcc tccagccagt cgagatgctg
     5101 gtcgatcatc ctctgcacgg tcgcccgcgc cgggcggccg agcaggccga ggcgccggac
     5161 gaccagcatg acgagcagcg gcggcatcag cgagccttcc gcataatgga ggaagtggcg
     5221 gtagcgcagg acctcctcgc gccggcgcgg gccgaggcgg ccgtccgcct tctcgaccag
     5281 atattcgacg atcgccccgg tctcggcgac cacgatgccc tcatcctcga tcaccggcga
     5341 cttgccgagc gggtggaccc gcctgagctc gggcggagcg agcatcgtct tcctgttgcg
     5401 ctcgtagcga cgcacctcat agggcaggcc gagctcctcg agcagccaga ggacccgctg
     5461 cgagcggctg ttctcgagat gatggacgag gatcatcgat tgttcctgtg gcacagggcc
     5521 gcaacctcct tgaccagctt ggcggcgagg acggcggtga ctccgttcac gtcgcggacg
     5581 ggattatact cgacgacgtc ggcgccgacg atgggagcgc ggacccggtg gagcaccgcc
     5641 agcacgtcgc ggaccgacag gccgcccggc tcgtgatggg agacgcccgg ggcgaaggcc
     5701 ggatcgaagc cgtccaggtc gaggctgatg tagagcggcc cggtcggcaa gggcaccgcg
     5761 tcgggcgaga aatggcgcat ctcgacgatt tcgaccccga agcgctccgc ctgctcgcgg
     5821 caatggcggt tcagggtgcg gatcccgacc tggaccagcc ttcgggcgag gccccgctcc
     5881 atgatccgcg cgaagggcga ggcgtgggat cgggggttgc cctcgaaatc gtcgtacagg
     5941 tccggatggg cgtcgaaatg gaggatgtcg atcgcgccgt gggcggcggc gagcgcctcg
     6001 agcacgggga cggtgaccga atggtcgccg ccgaggacga gcgggacggc gccggcggcg
     6061 gccgcctcgg cgacggtggc ggcgatcagc gcgtcgtcgc cctcgccctc ggtgaggggc
     6121 aggtcgccgg catcggcgag gaaaacgtcc acgcccagct cgacccccga ttcggcggtc
     6181 aggttgccgt ggtccgaccc gagggcgtcg cggatcaggg gcggcgcctt ggccgcgccg
     6241 cgcaggaagg acgaatggcg gtcggtcggg gctccgatca gcctcagttc cagaatgctg
     6301 gctgtcgcgc cttcagtgtc catgcggcgg ctgtggagac gcggatcggg cttgtccagc
     6361 ggacggcgac cccggaacct ccaccgtcgc cccagcgaaa gctggggtcc atgccgaggc
     6421 cggatggatg ccagccttcg ctggcatgac ggtttcagga cacccacttt tcgggaaccc
     6481 tattcgcgcg ccatcgtcta tgcgcgttcc agcccaggga agaccatgct ccagccgccc
     6541 cgccccgacc ttgccgacgc ccgcccgggc ttccgcgagt tcgtcgtcct gatcgcgggg
     6601 ttgatgtccc tgaacgcgct tgccatcgac gcgatggtgc cggcgctccc ggcgatcggc
     6661 caggccctga acgtcgccga cgacaacagc cgccagctcg tcgtctcgct ctacgtgctc
     6721 ggcttcggcc tgacccagat aatctacggc ccgctgtcgg accggttcgg gcgcaagccg
     6781 gtgctggccg tcagcctcgt cctctacgtc ggcttcgccc tcgcctgctg ggcggcgccg
     6841 agcttcaccc tgctgctggc ggcgcggatg gcgcagggcg gcgccgctgc ctcgacccga
     6901 gtgctggtcg tctcgatcgt ccgcgaccgc ttcgaaggcc cgcgcatggc ccggatcatg
     6961 tcgctcgcct tcctcgtctt cctgctgatc ccgatgctgg cgccggtctt cggccagctc
     7021 actttgcggg tcgcctcctg gcgcgcgatc ttcttcgggc tggcgctggg cggagcgatc
     7081 atgctgacct ggacgctggt ccggctgccc gagacccttc gccccgaata tcggcgcccg
     7141 ctcgacgccg ggacgatctg ggatggaacc tgccagactc tgagcaaccg ccagtcgctc
     7201 ggctacacgc tcgccttcac tttgatgatg gcgagcctga tgggctacat taactcgatc
     7261 cagcagatcg tcttcgacat cttccaccgg cccgagctga tcgccgcgac cttcgccggg
     7321 atcgccctgc ccatggcctt cacctcctgg ctcaactcgc gcatcgtcga gcggatcggg
     7381 acccgccggg tcgcccatgc cgggctggcc ttgttcacgg cctcggcgct cgtccacctc
     7441 gccatcgtcc tgaccaccgg cgagagcctg tggctgttca tcggcctgca ggggctcacc
     7501 atggcctgct tcggcttggc ctcggccaat ttcggggcgc tggcgatgca gccgctgggc
     7561 cacgtcgccg gcaccgcctc ctcggtgcag ggaacgatcg ggacgatcgg cggcgccctg
     7621 ctcggactcg ccgtcggcca gagcttcgac ggcaccctgg tgccgatggt ggggagcttc
     7681 gccgccttcg gagccttcgg cctcgtcatc ctcctgtgga ccgagcgcgg tcggttgttc
     7741 gggcggggct gaacggcgcg aggcttcggg gtcgcggtgc gagctggatt ttcaggagaa
     7801 ggcgcgaagg cgcgaaggcg cgaagcgcaa tccggtgttg tgccgagcct ggctgcgcgt
     7861 cgctcggcgc ccgcttcgtg ccttcgtgcc ttcccttgga atcaatcggc cacggccttc
     7921 ccgtcaggcc gcgcgggacg caccgacccc cgcgacgagg ccgttccgcg aggcaagacc
     7981 aggaagcgcc tggggcggcc gacggtgtga agcttgcgaa acttacacat gccatcggtg
     8041 ccgtaagctt cgccctctag acgtcgtcct ccgccgccgg ccccgccatc agcgcctcgg
     8101 cgacctcgtc ggtcttgccg cggatcgcgc gctcgagccg ggcggcgacg tccggattct
     8161 ccctcagata gttcttggcg ttctcgcggc cctggccgat ccggatcgaa tcgtagctga
     8221 accaggcgcc ggacttctcg accaggccgg ccttgactcc gaggtcgagg atctcgccga
     8281 ccttcgagat gccctcgccg tacatgatgt cgaactcgac ctgcttgaac ggcggcgcca
     8341 ccttgttctt gaccaccttc acccgagtgg tgttgccgac gatgtcgtcg cggtccttga
     8401 tctggccggt gcggcggatg tcgagccgga cgctggcgta gaatttgagc gcgttgccgc
     8461 cggtcgtcgt ctccggattg ccgtacatca cgccgatctt catgcgcagc tggttgatga
     8521 agatgacgag gcacttggac ttggagatgg acccggtcag cttacgcagc gactggctca
     8581 tcagccgcgc ctgcaggccg acatgggaat cgcccatctc gccctcgatc tcggccctcg
     8641 gcacgagcgc ggcgaccgaa tcgacgacga ggacgtcgat cgcgttggag cggaccaggg
     8701 tgtcggtgat ctcgagcgcc tgctcgcccg tgtcgggctg ggagacgatc agctcgtcga
     8761 cgtcgacccc gagcttgcgg gcatagacgg gatcgagcgc gtgctcggcg tcgacgaagg
     8821 cggccgtgcc gccgttcttc tgagcctcgg cgatgacgtg gagggcgagc gtggtcttgc
     8881 ccgagctctc cggcccgtag acctcgatca cccggccgcg cgggaggccg ccgatgccga
     8941 gcgcgatgtc gaggccgagc gatccggtcg agatggtctc gatctcgacc gcctcgcgcg
     9001 agccgagctt catcgcgctg ccttttccga acgcgcggtc gatttgagcg agggcggcct
     9061 cgagggcctt ctgcctgtcc atattgtccg agtttcccac caccttgagt gaagccgcca
     9121 tggccctcat cccctatgcc ctgcgcgccg cccattcaac gcgattgtca cgatgtacat
     9181 ggtccgttcc cggagaacaa gaggaaaacg gcccgcccgg cgtcgcgccc cgcatgcaaa
     9241 atccgcttgc aacgcggccc cctccgaatg agcgacccgc gcggttccag tcacttaccc
     9301 ttcggtgcct ggcaccgaac tcgcggtttt cggttttagc ggttaccagt cgatcattca
     9361 ccgcttttgc gctaggatcg gggcgatgcc gcgcagcgcc cgcctcaccg tccccggctt
     9421 gccgcaccac gtcacccacc gcggcaaccg ccgccagcgg accttcttca ccgaggccga
     9481 ccaccggctc tacgtccgct acctggcggc ggcctgcgcc gcgacgggca cggcggtctg
     9541 ggcctggtgc ctgatgccca accacgtcca cctggtgctc gtgccttcgg ccgaaggcgg
     9601 gctcagcgcc gtcctccacc gcacccaggg ccgctacacc agggcgatca acgcccgcga
     9661 gaaatgggac ggccagctct ggcagggccg cttcgcctcg ttcctcatgg acgagcgcca
     9721 cctcctcgcc tgcgcccgct acgtcgagct caatccggtg cgcgccggcc tggtcgcgcg
     9781 ggcggcggac tggccctggt ccagcgcgcg gacgcatctg ggcggggccg ccgacccgct
     9841 gaccgacccc gccccgctgc tcggccggtg gccggactgg gcgtcggtgc tcgactccgg
     9901 cgacgacgcg cggtcgctgc aggcgatccg ggagcacgag cgcagcggcc gcccgctcgg
     9961 aagcaaggcc ttcctcgagc ggctggaaag ggcgaccggc cggaccctcc ggccccggcg
    10021 gcgggtccgg attgcggcgc cccgcccggc ctgcgacgac ccgccgcggg cgccggggac
    10081 cgtaccgcct tgaccggccg cacggcgcct ggaaactcaa cggcttgacc ctcggtgcct
    10141 ggcgccgaac ggcgcggatc cggaagggcc ggcgcgtgga aatccaacgg cttgaccctc
    10201 ggtgcctggc accgaacggc gtggatccgg aagggccggc gcgtggaaat tcaacggctt
    10261 gacccttggt gcctggcacc gaacggcgcg gatccgcaag ggccggcccg tggaaattca
    10321 atggcttggc ctttggtgcc tggcaccgaa cggcgcggaa tcggaagagc ccgcgcttgg
    10381 aaattcaacg gcttgaccca tggtgcctgg caccgaacgg cgcggatccg gaagggccgg
    10441 cgcgtggaaa tccaatggct tgaccctcgg tgcctggcac ctaacggcgc ggatccgcaa
    10501 gggccggccg gtggaaattc aatggcttgg cctttggtgc ctggcaccga acggcgcgga
    10561 atcggaaggg ccggcgcgtg gaaattcaac ggcttgaccc tcggtgcctg gcaccgatgg
    10621 gcgcagatcc ggaaggacag gtgcgtggaa attcaatggc ttggcctttg gtgcctggca
    10681 ccgatccgcc accgatccgc accgatgcgc gccgtactgg ccggtcccgc gccgatcctg
    10741 gggcttcgcg ccttgcccgc cttggccttt gctggctagt ctgggatttc cgtcctcgga
    10801 aagggagccc atgccgatcc tgtccggatc cctccgtgcc tttctcgccg cgctggcggg
    10861 tctcgccgcg cttgccgttc ccgccgccgc cgcgggccag acgcgcggcc aggcggcgaa
    10921 ggaccccgcc caggcgatct tcgagcgctg ccggtcgccc gacttcgcga aggcgaggga
    10981 ctgctatgcg gccgcgctcg acgatcggct tgcgaccggc gacccggcgg cggcgctgca
    11041 gctgctcgac ggcctcaccg cgctcgaccc agaggtgcgc cggcacgggc atttctacgc
    11101 gcaccggatc ggcatctccg cgctcaagtc ccccgccgag gtgggccggg tcttcgcggc
    11161 ctgcacgccg gcctacgaat cgggctgcta ccacggcgtc atccagtcct acttcatcgc
    11221 cacggagcgg gacggcggcg gagtcacgac gcaaagcctc gacgcgctgt gcggagacta
    11281 tcggggcaag cgcgcggacc tgctcttcca gtgcacccac ggcctcggcc a
//
LOCUS       DASVQZ010000263         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_154222, whole genome shotgun sequence.
ACCESSION   DASVQZ010000263 DASVQZ010000000
VERSION     DASVQZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154222"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2128)
                     /locus_tag="VF547_07245"
     CDS             complement(<1..2128)
                     /locus_tag="VF547_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262652.1"
                     /translation="MPGPVPSPDSARCRAALLLGAAAIALGTAERASAQAFQGNPTVA
                     AGFATRTTAPGTETITVQTPSAIINWDPTDVAGVGTIDFLPAGNVATFQNGPNNPDFV
                     VLNRILPLNPARAIALNGTVISQLQNSGNTVRGGTIAFYSPGGILIGGKAVIDVGSLL
                     LTTIEPEIVGSGEFFVGNRYNLVKSDPNSFITVEAGASIKALEEKSYVALASPRILQQ
                     GDVRVNGAAAYVAAEAAALTIDNGLFDIEVAVGSSSALNTLVHEGATGGPASGGAGDN
                     HRIYMVAVPKNSAITALLSGSAGFDAAADAAVVNGEIVLSAGRNVVTETFFDVLKLPP
                     SSFEILGGTYNSSIRGSATADFLAGTKTDLTVNGNLVLQGDAHAHLLANGTTAAVKGN
                     LELRSDIILGEAGPGDPLDAKAGEAAIITSGNAQVTVTGSTLISASAEGGFFDAGNAA
                     GSGTGGTARITAAGGKVDLLGTLTIAADGRAAIGATTPAAAAAGTGGSASVTALTGAS
                     ITVGNETEMTANGISSRSDLAGPGGAGSGGSVTLAATSGGTVTLAGALAATAQGQGGG
                     NENAGGAGGSGTGGKIIIAASGGGLDLQGGGFARSLGTGGQGMVGGAGTGGAASVEAT
                     AGTVKLGGGFELAASGQGGDPGLLDGGRGGDGIGGTAIVQPHSSDSAGSIAAGDLFVV
                     AEGKGGAGSLGSGASGGGRGGDGTGGY"
     gene            complement(2128..>3192)
                     /locus_tag="VF547_07250"
     CDS             complement(2128..>3192)
                     /locus_tag="VF547_07250"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX8262653.1"
                     /translation="GRWGALVRGETYGLTGLGDRTTLAAFTTADFDEQQTVQIGHDFR
                     IGGEGLTLGAMLTHAWAEPELDDPALRIRAKTLLATLEATYPLIRTQATGLRAGAGLD
                     LIDQRVRFNGLELSRDRLRVAFARLELDRSDPASFGLRPGYNAAEPRWRLAGRIELRQ
                     GLDLFGASPSCGPAFAGCTAPGAVPPGRLEGDSGGTSLRFEAFGEFRPRPRLALVAGL
                     RGQMTGDPLLSFEQYSAGNYTIGRGYDPGTLLADRGLGVQAEIRYGSLVPSAPRRLAW
                     QAFAFLDAARVSEAGRIPSLTGGRNLASAGAGLRAMLGDGARIEAMLAVPLERAGVPA
                     VRPDPRLLLSFTTRLWPWSH"
BASE COUNT          404 a         1070 c         1273 g          445 t
ORIGIN      
        1 cgtagccgcc ggtgccgtcg ccgccgcggc cgccgccgga ggcgccgctg ccgaggctgc
       61 cggcgccgcc cttgccctcg gcgacgacga acaggtcgcc cgcggcgatc gagccggccg
      121 aatcgctgct gtgcggctgg acgatcgcgg tgccgccgat gccgtcgccg ccgcggccgc
      181 cgtcgagcag gccggggtcg ccgccctggc cggaggccgc gagctcgaag ccgccgccga
      241 gcttgaccgt gcccgccgtc gcctcgaccg aagcggcgcc gccggtgccg gcgccgccga
      301 ccatgccctg gccgccggtg ccgagcgagc gcgcgaagcc gccgccctgg agatcgagcc
      361 cgccgccgct ggcggcaatg atgatcttgc ctccggtccc cgatccgccg gcgcccccgg
      421 cattctcgtt gccgccgccc tggccctgag cggtggcggc aagcgccccg gcaagggtga
      481 ccgtcccgcc gctggtcgcc gcgagggtga cggagccgcc gctgccggcc ccgcccggtc
      541 cggcaagatc ggaccggctc gagattccgt tggcggtcat ctcggtctcg ttgccgacgg
      601 tgatcgaggc gccggtgagc gcggtcaccg aggcgctgcc gccggtcccg gccgctgccg
      661 ccgcgggggt cgtggcgccg attgcggcgc ggccgtcggc ggcgatggtg agagtgccga
      721 gcaggtcgac ctttccgccc gcggcggtga tccgcgcggt gccgccggtg ccgctgccgg
      781 cggcgttgcc ggcatcgaaa aagccgccct cggcgctggc gctgatcagg gtgctgccgg
      841 tgacggtgac ctgggcgttg ccggaggtga tgatggccgc ctccccggcc ttcgcgtcga
      901 gcggatcgcc cgggccggct tcgccgagga tgatgtcgga gcgaagctcg agattgcctt
      961 tgacggcggc ggtggtgccg ttggcgagga gatgcgcgtg cgcgtcgccc tgcagcacga
     1021 ggttgccgtt gacggtcagg tccgttttcg tacccgccag gaaatcggcc gtcgcgctac
     1081 cgcggatcga gctgttgtac gttccgccga ggatctcgaa gctcgacggc ggcagcttta
     1141 gcacgtcgaa gaaggtctcg gtgacgacat tgcgtccggc cgacaggacg atctcgccgt
     1201 tgacgacggc cgcgtcggca gccgcgtcga agccggcgct gccgctcagc aaggcggtga
     1261 tcgccgaatt cttgggcacg gcgaccatgt agattcgatg gttgtcgccg gcgccgccgc
     1321 tggccggccc gccggtggcg ccctcgtgga cgagggtgtt gagggcgctg ctgctgccca
     1381 ccgcgacctc gatgtcgaac aggccgttgt cgatggtgag cgcggccgct tcggcggcga
     1441 cataggcggc ggcgccgttc accctgacgt ccccctgctg gaggattcgc ggcgaggcga
     1501 gggcgacgta gctcttctcc tcgagcgcct tgatcgacgc gccggcctcg acggtgatga
     1561 agctgttggg atcggatttg acgaggttgt agcggttgcc gacgaagaac tcgccgctgc
     1621 cgacgatctc cggctcgatg gtggtgagca ggaggctgcc gacgtcgatc acggccttgc
     1681 cgccgatcag gatcccgccg gggctgtaga aggcgatcgt gccgccccgc accgtgttgc
     1741 cggagttctg aagctggctg atcacggttc cgttcaaggc gatggcgcgc gccgggttga
     1801 gcgggaggat ccggttgagg acgacgaaat cggggttgtt cggcccgttc tggaaggtgg
     1861 cgacgttgcc ggccggcagg aagtcgatcg tcccgacccc ggccacgtcg gtcgggtccc
     1921 agttgatgat ggcgctcgga gtctggacgg tgatcgtctc ggtgcccggc gccgtggtgc
     1981 gggtggcgaa gccggctgcg acggtgggat tgccctggaa ggcctgggcc gaggcccgct
     2041 cggcagtgcc cagggcaatg gcggcggcgc cgagcagcag ggccgcgcgg cagcgggcag
     2101 aatcggggga ggggacgggg ccgggcatca gtggctccat ggccagaggc gggtggtgaa
     2161 ggacaggagg aggcggggat cgggccggac ggcggggact ccggcgcgct cgagcggcac
     2221 cgccagcatg gcctcgatcc gggcgccgtc gccgagcatg gcgcgcaggc ccgcgcccgc
     2281 cgaggcgagg ttgcggccgc cggtgagcga ggggatccgg cccgcctcgg agacccgggc
     2341 cgcgtcgagg aaggcgaagg cctgccaggc gagccggcgc ggcgcggagg ggacgaggct
     2401 gccgtagcgg atctcggcct ggacgccgag gccgcggtcg gcgagcaggg tgccgggatc
     2461 gtagccgcgc ccgatcgtgt agttgccggc ggaatattgc tcgaagctca gcagggggtc
     2521 gccggtcatc tggccgcgaa ggccggcgac gagggccagc ctcggcctcg ggcgaaattc
     2581 gccgaacgcc tcgaagcgca gcgaggtgcc gccggaatcg ccttcgagcc ggcccggcgg
     2641 aaccgcgccc ggagcggtgc agccggcgaa cgcgggaccg caggacgggc tggcgccgaa
     2701 caggtcgagg ccctggcgga gctcgatccg cccggcgagg cgccagcgcg gctcggcggc
     2761 gttgtagccg gggcggaggc cgaagctggc ggggtcggag cggtcgagct cgaggcgggc
     2821 gaaggcgacg cgcagcctgt cccgcgacag ctccaggccg ttgaagcgga cgcgctggtc
     2881 gatcaggtcc aagccggcgc ccgcccgaag gcccgtcgcc tgggtgcgga tcagcggata
     2941 ggtcgcctcg agggtggcga gcagggtctt cgcccggatg cgcagcgccg gatcgtccag
     3001 ttccggctcg gcccaggcgt gggtgagcat ggcgccgagg gtcaggcctt cgccgccgat
     3061 gcggaagtcg tggccgatct gcaccgtctg ctgctcgtcg aaatcggcgg tggtgaaggc
     3121 ggccagagtc gtcctgtcgc cgagcccggt caggccgtag gtctcgccgc ggaccagcgc
     3181 gccccagcgg cc
//
LOCUS       DASVQZ010000264         5199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_533800, whole genome shotgun sequence.
ACCESSION   DASVQZ010000264 DASVQZ010000000
VERSION     DASVQZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533800"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VF547_07255"
     CDS             complement(<1..1421)
                     /locus_tag="VF547_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HEX8262654.1"
                     /translation="MDGLYEQVRIAIHQVWRRRWLALAVAWGLCLVGWLAVALIPNTY
                     ESKARVFVQMQSILPNQVGITAQEREGDLNRVKQTLTSSENLAKVVRRTELNNLVASE
                     RDLAVQVAGLRESIKVVAQPDNMFEISATARVSGFSNRQNAKLSAAVVQGLLDIFVEQ
                     NLVGDREEANQSLAFLDQQLKAREQQLQEAEQRRVEFEQRYLGLLPGEGSIGQRMGAA
                     RTELAGIEQQLMQAQSSLAALRSQLAATPSSMPALGIDSGGGGARGQLAVLEGQLSQA
                     AAQGWTDSHPDVVRLKNQIARLRPIAAREPVGAGTQPNPSWVSLRAMVAEKEGQVQAA
                     AARKAQLQSDLAQLGNRQASEPGVAAEQERLNRDYDVLKRQYDKLLEDRDQVRLRSDV
                     RTQTDAVKFRVIDPPSQPSVPMTPNRPLLLTLILLVGLGAGAGTAYVLGQIQTTFPTQ
                     TRLADATGLPVLGSISEVFTEVTR"
     gene            complement(1471..2055)
                     /locus_tag="VF547_07260"
     CDS             complement(1471..2055)
                     /locus_tag="VF547_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEX8262655.1"
                     /translation="MAADAKPANSLPPASFVGSDDTAPTEKYVIGALDQLNIFVWRNA
                     DLGGKVQVRPDGMITTPLIPDLQAAGKTPAQLADDIRLALTKYVDDPRVTVMVESFQG
                     SFSQQIRVVGATEKPASIPYRANMTLLDAMIAVGGLSEYAAGNRARLIRHDKASGTQK
                     EYNLKIARLLKKGDTSANVRLEPGDVIIIPESMF"
     gene            complement(2369..3601)
                     /locus_tag="VF547_07265"
     CDS             complement(2369..3601)
                     /locus_tag="VF547_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HEX8262656.1"
                     /translation="MSKPMGAIPQGFAAEQGMLLIAGRRADSLVEEAGDTPLFLYDVG
                     KVAAQVDLFRSHFPGVNLHYAIKANTYAPLLSRVANMVDGLDVASAGEMEAALAAGTD
                     ASEISFAGPGKRDDELAAAIAAGATLNLESEGEAERALAIGRAQGRRPRLAVRVNPDF
                     EIKGSGMRMGGGAKPFGVDSARVPALVRRLIEGGAEWRGFHIFAGSQALAAEALIEAQ
                     RATVELAAELAAAVGATPPLVNLGGGFGIPYFATESPLNAREVGERLVATLRERPPIL
                     AASGFAIELGRWLVGEAGVYLTRIVDRKESHGKTSLVTDGGLHHQLAASGNFGQVVRR
                     NYPVAIASRFGAPAEEEASIVGCLCTPLDRLADDVMLPRAEVGDLVAVFLAGAYGLSA
                     SPAAFLGHPPAREMVVGG"
     gene            complement(3598..5106)
                     /locus_tag="VF547_07270"
     CDS             complement(3598..5106)
                     /locus_tag="VF547_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HEX8262657.1"
                     /translation="MQVPDPTPRPLDHLTLRGAAGAPALLTKDGLLDYAGLEAAVGTL
                     AAALKALGFPPGARIASWLPKTRMTSLLPLACARAGLVHVPVNPLLKRAQVAHILADS
                     GARLLISGKARLATLAEGDLPAGCAAVGEEEGAAMLSGADRLPPSDADPDDLAALLYT
                     SGSTGRPKGVMLSHANMWLGAVSVAHYLRLAPPDRVLGVLPLSFDYGQNQLLSTWAAG
                     ACVVPIEFLTARDVIKAVEAQGITTLAGVPPLWVQLVEAPWPPRAALSLRRLTNSGGR
                     LPVSVVRRLRELFPAAELYLMYGLTEAFRSTYLDPALLDEHPDSIGTAIPFAEVMTAR
                     PDGTLCAPGEDGELVHAGPLVAKGYWNDPERTAQRFRPAPAGSRFGGTAVWSGDRVRA
                     DGQGLLYFVGREDSMIKTSGNRVSPTEVEEAAVASGVVAEAVALGVPDDRLGEAIALV
                     VRGEGPDEALRGFLRRELPNFMQPAHILWRDELPRSPNGKLDREAIRSELIQ"
BASE COUNT          755 a         1777 c         1872 g          795 t
ORIGIN      
        1 cgggtcacct cggtgaacac ctcggagatc gagccgagca cgggcaggcc ggtcgcgtcg
       61 gcgagccggg tctgggtcgg gaaggtggtc tggatctggc cgagcacata ggccgtcccc
      121 gccccggcgc cgaggccgac gagcaggatc agggtgagca gaagcggccg gttcggagtc
      181 atcgggacgc tcggctggct cggcggatcg atcacccgga acttgaccgc gtcggtctgg
      241 gtgcggacgt cggagcgcag gcggacctgg tcgcggtcct ccagaagctt gtcatattgc
      301 cgcttgagga cgtcgtagtc gcggttgagc cgctcctgct cggcggcgac cccgggctcg
      361 gacgcctggc ggttgccgag ctgggcgagg tccgactgga gctgcgcctt gcgggcggcg
      421 gcggcctgca cctgcccctc cttctcggcg accatcgccc tcagcgacac ccaggagggg
      481 ttgggctggg tgcccgcccc caccggctcg cgcgcggcga tcggcctcag gcgggcgatc
      541 tggttcttga gcctgaccac gtcgggatgc gaatcggtcc aaccctgcgc cgcggcctgg
      601 gaaagctggc cctccagcac ggcgagctgg ccgcgcgctc cgccgccgcc gctgtcgatg
      661 ccgagcgccg gcatcgacga gggggtggcc gcgagctggc tgcgcagcgc ggcgagcgag
      721 ctctgcgcct gcatcagctg ctgctcgatg ccggccagct cggtgcgcgc cgcgcccatg
      781 cgctggccga tcgagccctc gccggggaga aggccgagat agcgctgctc gaactcgacg
      841 cggcgctgct cggcttcctg gagctgctgc tcgcgcgcct tcagttgctg gtcgaggaag
      901 gcgaggctct ggttggcctc ctcgcggtcg ccgacgaggt tctgctcgac gaagatgtcg
      961 agcaggccct ggacgaccgc ggcggagagc ttggcgttct gccggttgga gaagccggag
     1021 acccgggccg tggcgctgat ctcgaacatg ttgtcgggct gggcgacgac cttgatcgat
     1081 tcgcggagcc cggcgacctg gacggcgagg tcgcgctcgc tggcgacgag gttgttcagc
     1141 tcggtgcggc ggacgacctt ggcgagattc tcgctcgagg tcagggtctg cttgacccgg
     1201 ttgaggtccc cttcccgctc ctgcgccgtg atcccgacct ggttgggcag gatcgactgc
     1261 atctggacga acacccgcgc cttcgattcg taggtgttgg ggatgagcgc gaccgccagc
     1321 cagccgacca ggcacaggcc ccaggcgacg gcgagggcca gccagcggcg gcgccacacc
     1381 tggtggatcg cgatccgtac ctgttcgtag agtccgtcca tcgaggctgg cgttccttgg
     1441 gtcagaggct gggggcggcg gcggccgcgg tcagaacatg ctctccggga tgatgatgac
     1501 gtcgccgggc tcgagccgga cgttcgcgct cgtgtcgccc ttcttcagga ggcgggcgat
     1561 cttcaggttg tattccttct gggttccgct cgccttgtcg tggcggatca gccgggcgcg
     1621 gttgcccgcc gcatattcgg agaggccgcc aacggcgatc atcgcgtcga gcagggtcat
     1681 gttcgcccgg tacgggatcg aggccggctt ctcggtcgct ccgacgaccc ggatctgctg
     1741 gctgaagctg ccctggaagc tctcgaccat caccgtgacg cgcgggtcgt cgacatattt
     1801 ggtcagcgcc aggcggatgt cgtcggcgag ctgcgccggg gtcttgccgg cggcctggag
     1861 gtcggggatc agcggggtgg tgatcatccc gtcgggacgg acctgcacct tgccgccgag
     1921 gtcggcgttg cgccagacga agatgttgag ctggtcgagc gctccgatca catatttctc
     1981 ggtcggggcg gtgtcgtccg agccgacgaa gctcgccggc ggaagcgagt tggcgggctt
     2041 ggcgtcggcc gccatggcgg cgccgagacc gagggcgaga acgagcgtgg aaccggccag
     2101 tttacgcatg ggatcaatcc tcttcctgtc gccgcggacc cccaccctga cgtcagggcg
     2161 cgaaatttcc ggtccgctat cgcctgaaga ggtaaaatta gcgttaggaa caatggcctt
     2221 gtgagacgcg tcccagatcg ggactgggca ccgcggaagg ttaattccct cggagaagcg
     2281 ccgtcattgc gaggagcgaa gcgacgcggc aatccagttc tgcctcgccc gctgcactgg
     2341 attgcttcgc tacgctcgca atgacggctt agccgcccac caccatctcc cgcgccggcg
     2401 ggtgccccag gaacgccgcc gggctcgccg acaggccgta ggcgcccgcc aggaacacgg
     2461 cgacgaggtc gcccacctcc gctcggggca gcatgacgtc gtcggcgagc cggtcgagcg
     2521 gcgtgcacag gcagccgacg atgctcgcct cctcctcggc cggcgccccg aagcggctgg
     2581 cgatcgcgac cggatagttg cgccgcacga cctggccgaa attgcccgag gcggcgagct
     2641 ggtggtgcag tccgccgtcg gtgaccaggg aggtcttgcc gtggctctcc ttgcggtcga
     2701 cgatccgggt cagatagacc ccggcctcgc cgaccagcca gcggccgagc tcgatcgcga
     2761 agccgctcgc cgcgaggatc ggcgggcgct cgcgcagcgt cgcgaccagc ctctcgccga
     2821 cttccctggc gttcagtggc gattccgtgg cgaaataggg gatcccgaat ccgcccccca
     2881 gattgaccag cggcggggtc gctccgaccg ccgcggccag ctcggcggcg agctcgaccg
     2941 tggcgcgctg ggcctcgatc agagcttcgg cggcgagcgc ctgcgagccg gcgaagatgt
     3001 ggaagccgcg ccactcggcg ccgccctcga tcagccgccg caccagcgcc gggacgcgcg
     3061 ccgaatcgac cccgaacggc ttggcgccgc cgcccatccg catgcccgag cccttgatct
     3121 cgaaatcggg attgaccctg accgccagcc tcggccgccg cccctgggcg cggccgatcg
     3181 ccagcgcccg ctccgcctcg ccttccgatt cgaggttgag ggtcgccccc gcggcgatcg
     3241 ccgcggcgag ctcgtcgtcg cgcttgccgg gaccggcgaa cgaaatctcc gaggcgtcgg
     3301 tgccggcggc cagggccgcc tccatctcgc cggccgaggc gacgtcgagc ccgtccacca
     3361 tattcgcaac tctactcagc aacggtgcat aagtatttgc tttgatggca taatgaagat
     3421 ttacgcccgg aaagtgactc cggaagagat caacttgcgc ggcaactttg ccaacatcgt
     3481 agaggaagag cggcgtatcg ccggcctcct cgaccagcga gtcggcgcgg cggcctgcga
     3541 tcaggagcat gccctgctcg gccgcgaagc cctgcggaat cgcccccatc ggcttgctca
     3601 ttggatcagc tcgctcctga tggcttcgcg gtcgagcttg ccgttgggag agcgcggcag
     3661 ctcgtcgcgc cagaggatat gagcgggttg cataaaattc ggtaactcgc gccgcaaaaa
     3721 gccccgcagc gcctcgtccg gcccctcccc gcgaacgacc agagcgatcg cctcgccgag
     3781 ccggtcgtcg ggaactccga gcgccaccgc ctcggcgacg actccgctcg ccaccgcggc
     3841 ctcctcgacc tcggtcgggc tgacccggtt gccgctggtc ttgatcatcg agtcctcgcg
     3901 gccgacgaaa tagagcaggc cctgcccgtc ggccctgacc cggtcgccgg accagaccgc
     3961 ggtgccgccg aagcgcgatc ccgccggcgc gggccggaag cgctgggcgg tgcgctccgg
     4021 gtcgttccaa tagcccttgg cgacgagcgg cccggcatgg accagctcgc cgtcctcgcc
     4081 cggcgcgcag agggttccgt cgggccttgc cgtcatgact tcagcaaagg ggatggcggt
     4141 gccgatcgag tcgggatgtt cgtcgagcaa ggcgggatcg agataggtcg agcggaacgc
     4201 ctcggtgagg ccgtacatca gatagagctc ggcggccggg aacagctcgc gcagccggcg
     4261 gacgaccgag acgggcagtc ggccgccgct gttggtcagg cgcctcagcg acagcgccgc
     4321 ccgcggcggc cacggcgcct cgaccagctg gacccagagc ggcggcaccc cggccagggt
     4381 ggtgatgccc tgcgcctcga cggccttgat gacgtcgcgg gcggtcagga actcgatcgg
     4441 aacgacgcag gcccccgccg cccaggtcga gagcagctgg ttctggccgt agtcgaagct
     4501 cagcggcagc acgccgagca cccggtccgg cggcgccagc cgcagataat gggcgacgct
     4561 gaccgctccg agccacatat tggcgtggct gagcatcacg cccttgggcc gcccggtcga
     4621 gcccgacgtg tagagcaagg cggcgagatc gtcggggtcg gcgtccgacg gcggcagccg
     4681 gtccgctccc gaaagcatgg cggcgccttc ctcctcgccg acggcggcgc agcccgccgg
     4741 caaatccccc tcggcgagcg tcgccaggcg cgccttgccc gagatcagca gccgggcgcc
     4801 gctgtccgcc aggatgtgcg cgacctgggc gcgcttgagc aggggattga ccggcacgtg
     4861 gaccagcccc gcccgggcgc aggcgagcgg gagcaggctc gtcatccggg tcttgggaag
     4921 ccaggacgcg attcgggccc cgggggggaa gccgagcgcc ttcagagccg ccgcgagcgt
     4981 tccgaccgcg gcctcgaggc cggcgtaatc gagcagcccg tccttggtca ggagggccgg
     5041 agcgccggcc gcgcctctca aggtcagatg gtcgagcggt cggggcgtcg gatcggggac
     5101 ttgcaccttg cctccttggt gcgccacagc aggaaggccg ggcggggagc cgagggggat
     5161 agcgcgttgg gcttcgaggt ggaactgttc gatagcctc
//
LOCUS       DASVQZ010000265         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_533981, whole genome shotgun sequence.
ACCESSION   DASVQZ010000265 DASVQZ010000000
VERSION     DASVQZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533981"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VF547_07275"
     CDS             complement(<1..1203)
                     /locus_tag="VF547_07275"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX8262658.1"
                     /translation="MTTRTETARIDPPIESRDDLLAPFERGEKPEPAWRIGTEHEKFV
                     YRRSDHRAPSYEEPGGIRDLLAALTEFGWKPVEEGGKVIALSGADGNVSLEPAGQLEL
                     SGAALENLHQTCAEAGRHLEQVKQVGERLDLGFLGLGMWPDKTRDELPRMPKGRYSIM
                     LRHMPRVGSLGLDMMLRTCTIQVNLDYSSESDMARKFRVGLALQPLATALFANSPFTE
                     GKPNGFLSFRSHIWSDTDPARTGMLPFVFEDGFGYDRYVDYALDVPMYFVYRDGRYLD
                     AAGLSFRDFLKGKLAVLPGEKPRSSDWTDHLSTAFPEVRLKSFLEMRGADGGRWSRIC
                     ALPALWVGLLYDQGALDAAWDEVRDWPLDERQRLRDDVPRLGLAARTPSGEPLRDLAA
                     RIVAIAEGG"
     gene            complement(1243..2007)
                     /locus_tag="VF547_07280"
     CDS             complement(1243..2007)
                     /locus_tag="VF547_07280"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX8262659.1"
                     /translation="MPATPAWPPSSLPRLYVAERLALGLTLTLDGAPANYLANVLRLG
                     PGAQARLFDDCTGEWLAQVAEAGKRRVTLTVLRHLRGREPVPDLWLLFAPIKRGRIDW
                     LVEKATELGVARLVPVLTRRTVVDRLNLDRLRAHIVEAAEQCGRTALTELAEPRKLEA
                     VLAAWPSDRALHFADEAGGGPMDARSGPAAILVGPEGGFTEEERAAIRALPQSAPITL
                     GPRILRADTAALAAVALWMRSAGDWTARPSSSASEE"
BASE COUNT          336 a          740 c          784 g          298 t
ORIGIN      
        1 gccgccctcg gcgatggcga cgatgcgcgc ggcgaggtcg cgcaacggct cgcccgaggg
       61 ggtccgggcg gcgaggccca gcctcggcac gtcgtcgcgc aggcgctggc gctcgtccag
      121 cggccagtcg cggacctcgt cccacgccgc gtcgagcgcg ccctggtcgt agaggaggcc
      181 gacccacaag gcgggaaggg cgcagatccg gctccagcgg ccgccgtcgg cgccgcgcat
      241 ctcgaggaag ctcttcagcc gcacctcggg gaaggcggtg gagagatggt cggtccagtc
      301 cgacgagcgc ggcttctcgc ccgggaggac ggcgagcttg cccttcagga aatcgcggaa
      361 ggagaggccg gcggcgtcca ggtagcggcc gtcgcggtag acgaagtaca tcggcacatc
      421 gagcgcgtag tcgacatagc ggtcgtagcc gaagccgtcc tcgaagacga agggaagcat
      481 gccggtccgc gccggatcgg tgtccgacca aatgtggctc cggaaggaga ggaaaccgtt
      541 cggcttccct tccgtgaagg gcgagttggc aaagagcgcg gtggcgagcg gctggagggc
      601 caggcccacc cgaaactttc gcgccatatc ggattcggat gaatagtcga gattaacctg
      661 aatggtacag gtccgaagca tcatgtcgag ccccaggctg cccacgcgcg gcatgtggcg
      721 gagcatgatc gagtagcggc ccttcggcat cctgggcagc tcgtcccggg tcttgtcggg
      781 ccacatgccg aggccgagga agccgaggtc gagccgctcg ccgacctgct tgacctgctc
      841 gagatggcgt ccggcctcgg cgcaggtctg gtggaggttc tcaagcgcgg cgccggagag
      901 ctcgagctgg cccgccggct cgaggctgac gttgccgtcc gcgccggaga gcgcgatgac
      961 cttgccgcct tcctcgacag gcttccagcc gaactcggtc agagccgcca gcagatcgcg
     1021 gatgcccccc ggctcctcat aggacggcgc gcggtggtcg gagcggcggt agacgaactt
     1081 ctcgtgctcg gtcccgatcc gccacgccgg ctccggcttc tcgccgcgct cgaacggcgc
     1141 gagcagatcg tcgcggctct cgatcggcgg atcgatgcgg gcggtttcgg tccgggtcgt
     1201 catcgccgcc ctctagcggc ggccgggaag ggccgccagc acctattcct ccgatgcgga
     1261 ggacgacggc cgggcggtcc agtccccggc ggacctcatc cacaaggcga ccgccgccag
     1321 agcggcggtg tccgcacgca ggatccgcgg tccgagcgtg atcggcgccg actgcgggag
     1381 ggcgcggatc gcggcccgct cctcctcggt gaacccaccc tccggtccga cgaggatcgc
     1441 cgcgggtccg gaccgtgcgt ccatcggccc gccgccggcc tcgtcggcga agtggagcgc
     1501 ccgatccgac ggccaggcgg cgagcaccgc ctcgagcttg cgcggctccg ccagctcggt
     1561 cagcgccgtc cgcccgcatt gctcggccgc ctcgacgata tgagcccgca gccggtcgag
     1621 attgagccgg tccacgaccg tgcggcgggt gagcacgggc acgagccggg cgactccgag
     1681 ctcggtcgcc ttctccacca gccagtcgat ccgcccacgc ttgatcgggg cgaagaggag
     1741 ccagaggtcc ggcaccggct cgcgcccgcg cagatgccgg agcacggtca gcgtcacacg
     1801 cctcttcccc gcctcggcga cctgcgcgag ccattcgccc gtgcagtcgt cgaacagcct
     1861 ggcctgggcg ccggggccga ggcggaggac gttggccagg tagttggccg gagctccgtc
     1921 cagggtgagg gtcaggccca gcgcaagccg ctcagcgacg tacagtcgtg ggagactcga
     1981 cggcggccag gcgggggtgg cgggcaagct tgggcatccc aatccgttcg ggctgggcct
     2041 gtcgaagccc gttccttctc caagcgcaag agaaagaatg gccttcgaca agcccaggcc
     2101 gaacggtaag gtcggggcag atgagcgttt ccaacgacag cgacatcgtc ccggacag
//
LOCUS       DASVQZ010000266         5046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_154837, whole genome shotgun sequence.
ACCESSION   DASVQZ010000266 DASVQZ010000000
VERSION     DASVQZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154837"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VF547_07285"
     CDS             <1..1042
                     /locus_tag="VF547_07285"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8262660.1"
                     /translation="EGRTEEALALARQAPADVEPERRAQLIGKLADETGDSKTAFEAF
                     REANAVAAEAPAAAQADPEGYQRRVQALTELVAPHWFRSWTPTSEDGRPAPVFLVGFP
                     RSGTTLLDTVLMGHPAVAVLEEEPILEAVSEALGGFERLPGLDAAEIGRLRALYFETL
                     DRLVPDAAARTIVDKLPLNILAAPLVHRLFPDARFVFAQRHPCDVVLSCFMQSFAIND
                     AMANFLDLGDSARLYDLVLTFWERCRDVLPLAVHTVRYEALVEDVEGEARTLLDFLGL
                     AWDPAVLDHRRTAEARGTISTPSYSQVIRPLYREASGRWERYRTQMAPVLPILAPWAL
                     RLGYGDIFEAEG"
     gene            1047..2645
                     /locus_tag="VF547_07290"
     CDS             1047..2645
                     /locus_tag="VF547_07290"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEX8262661.1"
                     /translation="MRDFSQLLDGLVYTRSRNAKLKLIGDYLKRTPDPDRGYALAALT
                     GELDLPGVKPAAIRAIVEERVDPTLFYMSWDYVGDMAETVSLLWPKRIDQPAEIDDGS
                     LRIANVVERLQTLGRAEAPGALARMLDHLDASGRYALLKLATGELRIGVSARLAKTAV
                     AQAFALDVDAVEEVWHGLGPPYLPLFAWAEGRGPQPTAEDVPVFRPFMLAHPLEALRV
                     SLADYAAEWKWDGIRVQIVRAGGETRLYSRAGDDIGRSFPEIAEAFDVDGAVDGELLV
                     KGEFQGADAHGGAAASFNALQQRLGRKLVSARMLKDYPAFVRLYDILFDGAEDLRPLP
                     WEKRRARLAAVVPRLDPERFDLSALIEAESFEALEEIRAGARDASIEGVMLKRRDSPY
                     VAGRRTGLWYKWKRDPLVADCVLMYAQRGSGKRSSYYSDYTFGCWTPEGELLPVGKAY
                     FGFTDEELMWLDRFVRNHTVQRFGPVREVEKSLVLEVAFDSIHASRRHKSGLAMRFPR
                     ISRIRTDKPAAEADTIEALKRLTT"
     gene            2780..3034
                     /locus_tag="VF547_07295"
     CDS             2780..3034
                     /locus_tag="VF547_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262662.1"
                     /translation="MASFIPEIQEEIFPHDPQNRGYCIQYRENEVNHCPGCGRTHWFV
                     GRVSAECGFCATALPLATASSRGAGTHSRGHRPMILRDAA"
     gene            complement(3175..3960)
                     /locus_tag="VF547_07300"
     CDS             complement(3175..3960)
                     /locus_tag="VF547_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HEX8262663.1"
                     /translation="MLRPVFVRLAAAAALALAAAAAPAQVAEIDPNTAIEEPAAAEAA
                     EPGWESDEYQPVDDGPAQSGPAESGYEGASAEGSAEAAAAGPAAGTASQRASAAAKGQ
                     TIPLKDVFGAAEGVFGKGAEGLADLIEKLLRERGEPSAYITGREAGGAFIAGVRYGSG
                     TLHHQIEGDRRVHWTGPSVGFDFGANADKIFVLVYNLHDSERLFRRYPAAEGNAYVVG
                     GFTASYLRRGDVVLIPVRLGIGARLGVNAGYMRFSQKNRWLPF"
     gene            complement(4122..4751)
                     /locus_tag="VF547_07305"
     CDS             complement(4122..4751)
                     /locus_tag="VF547_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX8262664.1"
                     /translation="MAKFDITPGLTFVSRDEWAPDPAHPRLGAIVPRDQRTHVFIHHT
                     DMEDEGDGTPNVWESESRMFAMMRDLQLCRPDLGLDVPYNFIAFISSINDGLYICEGR
                     GEDRAGAHTKGHNRAAIAVSFAGSFENRPIPSAEIEARMPLLSYFLGWLRYDPSHPAY
                     GNHSPMINLDRLRPDGRRVWYHRDVKNTDCPGVKLMAHLPQVRFERPPS"
BASE COUNT          744 a         1773 c         1749 g          780 t
ORIGIN      
        1 cgagggcagg acggaggaag cgctcgcgct cgcccggcag gcgcccgccg acgtcgagcc
       61 ggagcgccgg gcacagctga tcggcaagct cgccgatgag acgggcgact cgaagacggc
      121 gttcgaggcc ttccgcgagg cgaacgccgt cgccgcggaa gcgccggccg ccgcccaggc
      181 cgacccggaa ggctatcagc gccgggtgca ggccctgacg gagctcgtcg cgccccactg
      241 gttccggagc tggacgccga cctcggagga cggccggccg gccccggtct tcctggtcgg
      301 cttcccccgc tccggcacga ccctgctcga cacggtgctg atggggcacc ccgccgtcgc
      361 cgtgctcgag gaggagccga tcctggaggc ggtcagcgag gcgctgggcg gcttcgagcg
      421 cctgcccggc ctcgacgccg ccgagatcgg ccggctgcgg gcgctctatt tcgagacgct
      481 cgaccggctc gtccccgacg ccgccgcgcg aacgatcgtc gacaagctgc cgctcaacat
      541 cctcgccgcg ccgctcgtcc accgcctgtt tcccgacgcg agattcgtgt tcgcgcagcg
      601 ccacccctgc gacgtcgtcc tctcctgctt catgcagagc ttcgcgatca acgacgccat
      661 ggccaatttc ctcgacctcg gcgattccgc ccggctctac gacctcgtcc tcaccttctg
      721 ggagcgctgc cgggacgtgc tgccgctggc ggttcacacc gtccgctacg aggcgctggt
      781 cgaggacgtc gagggcgagg cccggaccct gctcgatttc ctcggcctcg cctgggaccc
      841 cgcggtgctg gaccaccgcc gcaccgccga ggcgcgcggc acgatcagca cgccgagcta
      901 ttcgcaggtg atccggcccc tctatcgcga ggcgagcggg cgctgggagc gttaccggac
      961 gcagatggcc cccgtcctcc cgatcctcgc cccctgggcg ctgcgactcg gctacggcga
     1021 catcttcgaa gcggagggct gagccggtgc gggacttctc gcagctgctc gacggcctcg
     1081 tctacacccg ctcgcgcaac gccaagctca agctgatcgg cgactatctg aagcgcacgc
     1141 ccgatccgga ccgcggctat gcgctggcgg ccctgaccgg cgagctcgac ctgcccgggg
     1201 tcaagccggc ggcgatccgc gcgatcgtcg aggaaagggt cgaccccacc ctcttctaca
     1261 tgagctggga ctatgtcggc gacatggccg agaccgtctc gctgctctgg cccaagcgga
     1321 tcgaccagcc ggccgagatc gacgacggct cgctgcgcat cgcgaacgtg gtcgagcgac
     1381 tgcagacgct cggccgggcc gaggcccccg gagcgctcgc ccggatgctc gaccatctcg
     1441 acgccagcgg ccgctacgcc ctgctcaagc tggccaccgg cgagctcagg atcggagtct
     1501 cggcgcggct cgccaagacg gcggtggcgc aggccttcgc gctcgacgtc gacgcggtcg
     1561 aggaggtctg gcacggcctc ggcccgccct atctgcccct gttcgcctgg gcggagggac
     1621 gcgggccgca gccgaccgcc gaggacgtgc cggtcttccg gccgttcatg ctcgcccatc
     1681 cgctcgaggc gctgcgggtc tcgctcgccg attacgccgc cgagtggaaa tgggacggga
     1741 tccgggtcca gatcgtccgc gccggcggcg agacccggct ctacagccgc gccggcgacg
     1801 atatcggccg ctcctttccg gagatcgccg aggcgttcga cgtcgacggc gcggtcgacg
     1861 gcgagctcct ggtcaagggc gagttccagg gcgccgacgc gcatggcgga gcggcggcga
     1921 gcttcaacgc gctccagcag aggctcggcc gcaagctcgt ctccgccagg atgctgaagg
     1981 actacccggc cttcgtccgg ctctacgaca tcttgttcga cggcgccgag gaccttcggc
     2041 cgctgccctg ggagaagagg cgggcgcggc tggcggcggt cgtgccgagg ctcgatcccg
     2101 agcggttcga cctgtcggcg ctgatcgagg cggagagctt cgaggcgctc gaggagatcc
     2161 gcgccggcgc ccgcgacgcc tcgatcgagg gggtgatgct gaagcggcgc gacagcccct
     2221 atgtcgccgg ccgccgcacc ggcctgtggt acaagtggaa gcgcgacccg ctcgtcgccg
     2281 actgcgtgct gatgtacgcc cagcgcggca gcggcaagcg ctcctcttat tattccgact
     2341 atacgttcgg ctgctggact ccggagggcg agctgcttcc ggtcggaaag gcctatttcg
     2401 gcttcaccga cgaggagctg atgtggctcg accgattcgt ccgcaaccac accgtccagc
     2461 gcttcgggcc ggtgcgcgag gtcgagaaga gcctggtgct cgaggtcgcc ttcgattcga
     2521 tccacgccag caggcgccac aagtccggcc tcgccatgcg cttcccgcga atctctcgga
     2581 tccgcaccga caagcccgcg gccgaggctg acacgatcga ggcgctgaag cggctgacca
     2641 cctgatcggc atcggccgcg cgattaacct ttgcgcgccg cctttccctt tttggaacgg
     2701 ccggaatcgg ggggctgaca agcgccggca agtggatcag gccttggctg ccgtagagcc
     2761 ttttcaaggg gttgcgacga tggcgagctt catcccggag atccaggaag agatcttccc
     2821 ccacgacccg caaaatcgcg gctattgcat ccagtaccgc gagaacgagg tgaaccactg
     2881 cccgggctgc gggcgcaccc actggttcgt cggccgggtg tccgccgaat gcggcttctg
     2941 cgcgaccgcg ctgccgctcg cgaccgcctc gagccgcggc gccggcaccc acagccgcgg
     3001 ccaccgcccg atgatcctga gggacgcggc ctgaggacaa gaattcctcc cctccctttg
     3061 caggggaggg ggaccatgcg aagcatggtg gaggggcttt gctgagtccg aaagaacccc
     3121 tccaccgcgc tgcgcgcggt ccccctcccc cggaaatccg ggggaggaat taatttagaa
     3181 gggcagccac cggttcttct ggctgaaccg catgtagccc gcattgactc cgagccgggc
     3241 gccgattccg agccggaccg ggatgaggac cacgtcgccc cgcctcaggt agctggcggt
     3301 gaagccgcca acgacatagg cattgccctc ggcggccgga tagcggcgga acagccgctc
     3361 gctgtcgtgg aggttgtaga ccagaacgaa gatcttgtcg gcattggcgc cgaagtcgaa
     3421 gcccaccgag gggccggtcc aatggacccg gcggtcgcct tcgatctggt ggtgaagtgt
     3481 gcccgagccg tagcggacgc cggcgatgaa cgccccgccc gcctcgcggc cggtgatgta
     3541 ggcgctcggc tcgccgcgtt cgcgcaggag cttctcgatg aggtcggcca ggccttcggc
     3601 gcccttgccg aacaccccct cggcggcgcc gaacacgtcc ttgagcggga tggtctggcc
     3661 cttggccgcg gcgctggcgc gctggctcgc cgtgccggcg gccggaccgg ccgcggcggc
     3721 ttcggccgaa ccctccgcgc tcgcgccctc gtagcccgac tccgcgggac ccgactgcgc
     3781 cgggccgtca tcgaccggct ggtattcgtc cgactcccag ccgggctcgg cggcttccgc
     3841 cgccgccggc tcctcgatgg cggtgttggg atcgatctcg gcgacctggg ccggcgccgc
     3901 cgcggcggcg agggccaagg cggcggccgc cgcgagcctc acgaagacgg gcctcagcat
     3961 gttcaaactc ccccttgcgc tccgggaagg aagctaagtc cgccggggct ttcgccgcaa
     4021 tgaacgggcg acgagacgag tcgttcttgc gccggaacgg ggcgcgaacc ccctttttgc
     4081 ggaggcccgc gccgacctct accgtaggac tccgggggcg atcaggaggg tgggcgctcg
     4141 aagcggacct gcggaaggtg cgccatcagc ttcacgccgg ggcagtcggt gttcttcacg
     4201 tcgcgatgat accagacccg ccggccgtcc ggcctcagcc tgtccaggtt gatcatcggc
     4261 gaatggttgc cgtaggcggg atggctggga tcgtagcgca gccatcccag gaagtaggat
     4321 aggagcggca tccgcgcctc gatctcggcc gaggggatgg gcctgttctc gaagcttccc
     4381 gcgaaggaca ccgcgatcgc cgccctattg tggcccttgg tatgggcgcc ggcgcgatcc
     4441 tcgcctctgc cctcgcatat gtacaggccg tcgttgatgc tgctgatgaa ggcgatgaaa
     4501 ttgtagggga cgtccagccc gaggtccggc cggcacagct gcaggtcccg catcatcgcg
     4561 aacatcctcg attccgattc ccagacgttg ggggtgccgt ccccttcgtc ctccatgtcg
     4621 gtgtggtgga tgaagacatg ggtcctttga tccctgggca cgatcgctcc gagacgcgga
     4681 tgggcagggt cgggagccca ttcgtcgcgg gagacgaagg tcaggccggg ggtgatgtcg
     4741 aacttcgcca ttgccaatag ctctcctgac tggatcgaca gggccgaggc cggttccggt
     4801 cgaagccagc ctcgcgccag cgagggctca ccctagggga cgagaggatt ttcccgccgt
     4861 gatccccgtc acgccacgtc cggcgctcgc ccggacggcg atccgccagc ggagagggtg
     4921 cggcgatttg gggggctgga accgccctgg tccgcgagga ccgcagccaa ggcgcttgcg
     4981 ccggcgtcgt ggcagcggcg ccagctggct ctggcggaga cggagggatt cgaaccctcg
     5041 gtagga
//
LOCUS       DASVQZ010000267         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_154894, whole genome shotgun sequence.
ACCESSION   DASVQZ010000267 DASVQZ010000000
VERSION     DASVQZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154894"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VF547_07310"
     CDS             complement(<1..940)
                     /locus_tag="VF547_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8262665.1"
                     /translation="MVAHVATVAFLGLEARAVEVQVQIASGLPAFVIVGLPDKAVAES
                     RERVRASLAAIGLALPPKRITVNLSPADLPKEGSHYDLPIAVGLLAAMGAVDRETIAD
                     YVVVGELGLDGRIAPSPGVLLAALHASSAGLGLICPASQGPEAAWAGTIEVIAAPDLI
                     SLLNHLRGGAALSPPRPGEAERAAGGLDLGQVKGQETAKRALEIAAAGGHNLLMSGPP
                     GAGKSLLAACLPGILPELTPSETLEVSMIASVAGQLVDGRLVRTRPFRAPHHSASMVA
                     LVGGGLKVRPGEVSLAHLGILFLDELPEFQRGALDSL"
     gene            1041..1376
                     /locus_tag="VF547_07315"
     CDS             1041..1376
                     /locus_tag="VF547_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262666.1"
                     /translation="MAWTDTWQRVSRTAPVRYTLVGVGCLLIALTPLVGPIPGPGGIV
                     VFAAGLSLVLKYSGWAKRLYVRFKRKHPNKGRWTDWGLRRASAARREELSKARETAAR
                     AEAQPYLGD"
     gene            1470..1604
                     /gene="rpmH"
                     /locus_tag="VF547_07320"
     CDS             1470..1604
                     /gene="rpmH"
                     /locus_tag="VF547_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8262667.1"
                     /translation="MKRTFQPSNLVRKRRHGFRARTATVGGRKILAARRARGRKSLSA
                     "
     gene            1607..>1890
                     /gene="rnpA"
                     /locus_tag="VF547_07325"
     CDS             1607..>1890
                     /gene="rnpA"
                     /locus_tag="VF547_07325"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8262668.1"
                     /translation="MTSAPPPPATLTRRADFLAANRGRRAPMPGFVLLVRPRGDEDMA
                     MRLGITVTKKIGNAVVRNRMKRRFRALAREVLPEGGVPGADHVLIGRSGGV"
BASE COUNT          286 a          673 c          658 g          273 t
ORIGIN      
        1 gcagcgaatc aagagcgccg cgctggaatt cggggagttc gtcgaggaac aggattccga
       61 gatgcgctag gctcacctcg cccggccgga ccttgaggcc gccgccgacc agtgcaacca
      121 ttgaagcact gtggtgcggc gcccggaacg gccgggtgcg caccagccgc ccgtcgacga
      181 gctgcccggc gacgctggcg atcatcgaga cttcgagggt ctcggagggc gtgagctccg
      241 gaagaatgcc cgggaggcat gcggcgagca gcgacttgcc cgcgcccggc gggccgctca
      301 tcagcaaatt gtggccgccc gcggccgcga tctccagcgc ccgcttcgcc gtctcctgcc
      361 ctttcacctg gccgaggtcc agcccccccg ctgcgcgctc ggcctcgcct ggccgcggcg
      421 gcgaaagcgc cgcgccgccg cgcaggtgat tgaggaggga aatgaggtcc ggcgcggcga
      481 tcacttcgat cgtgccggcc caggccgctt cgggcccctg cgacgccggg cagatcaggc
      541 cgagaccggc cgatgaggcg tgcagcgcgg cgaggagaac gccgggcgac ggcgcgatcc
      601 gcccgtcgag cccgagctcg ccgacgacga cgtagtcggc gatcgtctcg cggtcgaccg
      661 cgcccatggc ggcgagcagg ccgacggcga tgggaaggtc gtaatgcgag ccttccttgg
      721 gaaggtcggc gggcgagagg ttgacggtga tccgcttggg cggaagcgcg aggccgatgg
      781 cggcgagcga ggcgcggacc cgctctcggc tctcggccac cgccttgtcg ggcaggccga
      841 cgatgacgaa ggcggggagg ccgctcgcaa tctgcacctg cacttcgacg gcgcgtgcct
      901 cgaggcccag aaatgcgacc gttgcaacgt gcgcgaccat ccggaccccc gccattcatc
      961 cgtttcggat gaaacccggt ccgcctctga gcatttccga tcacttggcg aaagccccga
     1021 tcgacaccag attggaccta atggcctgga cggacacatg gcagagggtg agccggacgg
     1081 cgccggtgcg ctacacgctc gtcggcgtgg gctgcctgct catcgcgctg acgccgctcg
     1141 tcgggccgat ccccggtccc ggcggcatcg tcgtcttcgc cgccggattg agcctggttc
     1201 tcaagtatag cggctgggcg aagcggctct acgtgcgctt caagaggaag catccgaaca
     1261 aggggcgctg gacggactgg ggcctgaggc gcgccagcgc cgcgcgccgc gaggagcttt
     1321 cgaaggcacg cgagacggcg gcgcgggccg aggcccagcc ctatctcggc gattgacttt
     1381 gcggacgtgt ttgcatatcc gcccccaagc acggccgctt ccgttggatg cggccctttt
     1441 ctattcacgt acgtactgag gaatgagcga tgaagcgcac cttccagccg agcaatcttg
     1501 ttcgcaagcg ccgccacggc ttccgcgccc gcaccgccac cgtcggcggc cgcaagatcc
     1561 tggccgcccg ccgcgcccgc ggccgcaagt cgctttcggc ctaaagctga cgtccgcgcc
     1621 gcccccgccg gcgacgctta cccgccgcgc cgacttcctg gccgccaacc gcggccgccg
     1681 cgcgcccatg cccggcttcg tcctcctcgt gcgcccgcgc ggcgacgagg atatggcgat
     1741 gcgcctcggc atcacggtca caaagaaaat cggcaatgcg gtcgtccgca accgcatgaa
     1801 gcgccgcttc cgcgccctcg cgcgggaggt tctgcccgag ggcggcgttc ccggcgccga
     1861 tcacgtcctg atcggccgct cgggcggtgt
//
LOCUS       DASVQZ010000268         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_632186, whole genome shotgun sequence.
ACCESSION   DASVQZ010000268 DASVQZ010000000
VERSION     DASVQZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632186"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VF547_07330"
     CDS             complement(<1..509)
                     /locus_tag="VF547_07330"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8262669.1"
                     /translation="MGNRKYHRAVTEAVRGQGGHVAKFLGDGVLAYFGWPRAHEDDAE
                     RAVRAGLAVARAVEQLQTSAGPMAARMGIATGPVVVGEVLGEGEARERGVVGETPNLA
                     ARLQALAEPGAVVLDGATRRLIRGLFDYAELGEVGLKGVPRPMRAWRALGEGRVESRF
                     EALRAGAHAT"
     gene            603..2027
                     /locus_tag="VF547_07335"
     CDS             603..2027
                     /locus_tag="VF547_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX8262670.1"
                     /translation="MDAATDPWWESELAGCVFADARLGQRLHKLIERMGGAIGASLPL
                     ACQDWANTKAAYRFFSNDRVSEEQILAGHFQSTRERFAAAEGTILVLQDTTEFTYQRE
                     RPEAIGITYSVNSGKDKAGRFRMHTVCGLLMHASLAVTTDGLPLGLAAVKFWSRQKFK
                     GTAALKRKVNPTRVPVEHKESVRWLENMRQSTGLFGDPARCVHVGDRESDIYELFCTA
                     RELGTHFLVRSCVDRLAGDGGHTIAKEMNEAQVGGLHAVEVRDGKGRTETALVELKYR
                     RVHVLPPIGKQKRYPALALTVIHAKERATPADRPAIEWKLITDLPVATHEAAVEKLRW
                     YAQRWKIEVFHKILKSGCRAEEARLRTAERLVRLIAVFCILGWRVFWMTMMRRVAPDA
                     AAKLVLTGVEIALLDRLVPDKGHEPPGAGTLSSYLTKLARLGGYLARAKDPPPGSTVM
                     WRGLSRLTDIKLGVMLADAPPRCG"
BASE COUNT          340 a          797 c          766 g          312 t
ORIGIN      
        1 gtggcgtggg cgccggcgcg cagggcctcg aagcggctct cgacccggcc ctcgccgagc
       61 gcgcgccagg cgcgcatcgg gcgcggcacg cccttcagcc ccacctcgcc aagctcggcg
      121 tagtcgaaca gccccctgat cagccgccgc gtcgccccgt ccaacaccac cgccccgggc
      181 tcggcgagcg cctgcagccg ggccgcgagg ttgggcgtct cgcccaccac gccccgctcg
      241 cgcgcctcgc cctcgcccag gacctcgccc accaccaccg gcccggtggc gatccccatc
      301 cgggcggcca ttggcccggc ggaggtttgc agctgttcga ccgcccgcgc cacggccagg
      361 ccggcgcgca ccgcccgctc ggcgtcgtcc tcatgcgctc gcggccagcc gaagtaggcc
      421 agcacgccgt cgccgaggaa cttggcgacg tggccgccct ggccccgcac cgcttcggtc
      481 accgcccggt ggtactttcg attacccaca tcttcggtct gggttcaggt gcttggaggt
      541 tgcgcggcct gaaccgggcc cggtgctctg gccggcaccg aaatgcctga gggtgggtgc
      601 tcatggacgc tgcaaccgac ccgtggtggg aaagcgagct ggccgggtgc gtcttcgcgg
      661 acgcgcgcct gggccagcgc ttgcacaagc tcatcgagcg gatgggcggc gcgatcgggg
      721 cgagcctccc gctggcgtgc caggactggg ccaacaccaa ggcggcctac cgcttcttct
      781 ccaacgaccg ggtcagcgag gaacagatcc tggccggcca cttccagtcg acgcgcgagc
      841 gcttcgccgc ggccgagggc accatcctgg tgctccagga cacgacggag ttcacctacc
      901 agcgcgagcg ccccgaggcg atcggcatca cctacagcgt gaacagcggc aaggacaagg
      961 ccggccgttt caggatgcac acggtctgcg ggctcctcat gcacgcctcc ctcgccgtca
     1021 cgacggatgg ccttcctttg ggcctggctg cggtcaagtt ctggtcgcgg cagaagttca
     1081 agggcaccgc ggcgctcaag cgcaaggtca acccgacgcg cgtgccggtc gagcacaagg
     1141 aaagcgtccg ttggctcgag aacatgcgtc aatccacggg gctgttcggc gatcccgcga
     1201 gatgcgtcca cgtcggtgac cgcgagagcg acatctacga gctgttctgc acggcccggg
     1261 agctcggcac ccacttcctc gtcaggagct gcgtcgatcg cctggcgggc gatggcggcc
     1321 acaccatcgc caaggagatg aacgaggcgc aagtcggggg cttgcacgcc gtcgaggtgc
     1381 gcgatggcaa ggggcgcacc gagacggcgc tggtcgaact gaagtaccgc cgcgtccatg
     1441 tgctcccgcc gatcggcaag cagaagcgct atcccgcgct cgccctcacg gtgatccacg
     1501 ccaaggagcg cgccacgccg gcggatcggc cagcgatcga gtggaagctg atcaccgacc
     1561 tgcccgtcgc cacccacgag gcggcggtcg agaagctgcg ctggtacgcc cagcgctgga
     1621 agatcgaggt gttccacaag atcctgaagt cggggtgccg ggcggaggag gcgcggctca
     1681 ggaccgccga gcgcctggtg cgactgatcg cggtgttctg catcctgggc tggcgggtgt
     1741 tctggatgac gatgatgcgc cgtgtcgcgc cagacgccgc cgcaaagctt gtcctcacgg
     1801 gcgtggagat cgcgctcctg gaccgcctcg tgcctgacaa ggggcacgaa ccgccgggtg
     1861 ccggaacact ctcctcctac ttgaccaagc tcgcccggct cggcggctac ctggctcgcg
     1921 ccaaggatcc gccacccgga agcaccgtca tgtggcgcgg cctttcgcgg ctcactgaca
     1981 tcaagctcgg cgtcatgctc gcagatgctc caccccgatg tgggtgatcg aaagcttcac
     2041 cggagggata cgtaccaccc cgggcatatg ggcaagctga tgcgccggct cggcctgtcg
     2101 cggcggaagg cgcggccctc gcacccgaag gcggacgcgg cggcgcgcga ggccttcgcc
     2161 aagggggggc tgcaggcggg gctgcaggcc gccctcgacg acgcgcgggc ggagc
//
LOCUS       DASVQZ010000269         5714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_535310, whole genome shotgun sequence.
ACCESSION   DASVQZ010000269 DASVQZ010000000
VERSION     DASVQZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535310"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            595..873
                     /locus_tag="VF547_07340"
     CDS             595..873
                     /locus_tag="VF547_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262671.1"
                     /translation="MKKIAITAAAVLALGVAACNNSANDAGNNATDINATETEAGNDV
                     DNAAADANSAVSSTDNALDSIGDAAANTASDVGNAATDAGNAVANAVD"
     gene            988..1245
                     /locus_tag="VF547_07345"
     CDS             988..1245
                     /locus_tag="VF547_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262672.1"
                     /translation="MKTYGAMAALLAAASLLAACGQADDGSPTAAENEQLNNISEVLD
                     TSADSLVPEDGALGNGEAPAETGELPVVGNAVGNEALANGQ"
     gene            complement(1448..3097)
                     /gene="groL"
                     /locus_tag="VF547_07350"
     CDS             complement(1448..3097)
                     /gene="groL"
                     /locus_tag="VF547_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049566.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8262673.1"
                     /translation="MAAKDVRFSRDARERILRGVDILADAVKVTLGPKGRNVVLDKSF
                     GAPRITKDGVTVAKEIELKDKFENMGAQMLREVASKTNDLAGDGTTTATVLAQSIVRE
                     GMKSVAAGMNPMDLKRGIDLAVTRVVEDLKNRSKPVSGSNEIAQVGIISANGDTVVGE
                     KIAEAMEKVGKEGVITVEEAKGLEFELDVVEGMQFDRGYLSPYFITNPEKMQVELTDP
                     YILIHEKKLSNLQAMLPILEAVVQSGRPLLIIAEDIEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTNGEMISEDLGIKLENVTIAMLGQAKRVTIDKDNTTIVD
                     GAGEGDRIKARVEAIRQQIENTTSDYDKEKLQERLAKLAGGVAVIKVGGSSEIEVKER
                     KDRVDDALHATRAAVEEGIVPGGGVALLYATKALRGMSGVNDDQTRGIDIVRRALQSP
                     IRQIAENAGHDGAVVAGKLIDGDDETLGFNAQTEVYENLVQAGVIDPTKVVRTALQDA
                     ASVAGLLITTEAAVSEAPEDKSPAGGGMPGGGMGGMGGMDF"
     gene            complement(3209..3496)
                     /gene="groES"
                     /locus_tag="VF547_07355"
     CDS             complement(3209..3496)
                     /gene="groES"
                     /locus_tag="VF547_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8262674.1"
                     /translation="MNFRPLHDRVLVRRVEAEEKTAGGIIIPDTAKEKPQEGEVVAAG
                     SGTRGEDGTVTPLDVKTGDRILFGKWSGTEVRLDGEDLLIMKESDILGIVG"
     gene            3739..5610
                     /gene="sppA"
                     /locus_tag="VF547_07360"
     CDS             3739..5610
                     /gene="sppA"
                     /locus_tag="VF547_07360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX8262675.1"
                     /translation="MRFAGKVWKLLVGIKDGLVLLFMLLFFGLLYAAMTARPTLGAGD
                     RGALLLDLDGPIVEQPAQATASEVFSGGGPREYRLRDVVHALDTAATDDRVKAVALNL
                     DVFSGGGQATLAAAGEALDRVRKTGKPVLAFATAYDDDSYQLAAHASELWLDPMGGVL
                     VTGPGGANLYMKGLLDKLGVTANVYKAGTYKAAVEPFTRTDMSPEAREALTAVYGSMW
                     ESWQQEVRQARPKAQLAPYLADLPGRIEAAGGDMARAALQSGLVDRIGSREAFDRRLA
                     EIAGTRYAAVAGSFRRIPYRTWIEANPAGRDGGDIGILTVAGDIVDGEAGLGTAGAET
                     IVRALEKGLRERDLKALVLRIDSPGGSALASERIRQAVLAAKSKGLPVVVSMGSLAAS
                     GGYWVATAGDRIYAEPSTITGSIGVFGILPSFEGTMTKLGLGADGVKTTALSGEPDLL
                     RGPSPEADRLLQMGVDGTYRRFLALVSAARKMPVAEVDRIAQGRIWDGGTARQLRLVD
                     AFGGLDDAVREAAKRAKLDPDEAKAVWLEKQPDFSDRLLMSLAGDGDEEEAAPDAFSR
                     LADRPRIAVLTAIGEAERLLTGAAIQARCLECSGHAASRPRVASGGAAAWLARLFGL"
BASE COUNT          861 a         1994 c         1933 g          926 t
ORIGIN      
        1 gacaatggct gagcgtaaca gtccgtcacc cccttccggt caacagaggt ccgggtctcg
       61 ggtctctagc cgcgtccatc gcggacagac caatttcaag ctttcgtccg aagatcgaac
      121 aagtatatga tcgcttcgcc cgtgcctttc acaaggcttc ggtgatgcac cccctgaagt
      181 acaacgtcta cggcttcgaa ggccgaggcg gccgctatgt gtcgttgaga cgcgtctgcg
      241 gtcgaggcgc gcgacctgcg catcgcgcgg acggctcgcg tttcaaaacc accgtctgtc
      301 gcggcgagga cgagctgacg atgcgcgagc tggacgcgct cgccgacctc gatgacgctt
      361 cacccaggca gcgacgcgtc gcccctcggg cgaccgctcc gacccccgtt cgggcccggc
      421 cgggaagacg cccggacccg cggttgactc tcccccgccg ccccttcagt ctcgccgctt
      481 cggttcggcg caattgctta ccaaactagt agacatatcg ccgcgcctcc gataaatcca
      541 aagccgccgg acctcgcccg gcaaaactaa ccagtctccc aaggagcgaa tgccatgaag
      601 aagatcgcca tcactgccgc cgccgtcctg gcgctcggcg tcgccgcctg caacaacagc
      661 gccaacgacg ccggcaacaa cgcgaccgac atcaacgcca ccgagaccga ggccggcaac
      721 gacgtcgaca acgccgccgc cgacgccaac tccgccgtca gcagcaccga caatgcgctc
      781 gacagcatcg gcgacgccgc cgccaacacc gccagcgacg tcggcaatgc cgccaccgac
      841 gccggcaacg cggttgcgaa cgccgtcgac taaggcgagc ggttcctgac cgaatgagag
      901 ggtcgcgtgc cgccggcacg cggccctttt tatttgcgcc ggcttgcggc aagctggccg
      961 cgaggtcttt cgaggggaga gggttgaatg aagacatatg gtgccatggc cgcgcttctc
     1021 gccgccgcgt cgctcctcgc cgcctgcggc caggccgacg acggcagccc gaccgcggcc
     1081 gagaacgagc agctcaacaa catctccgag gtgctcgaca cgtcggccga cagcctcgtc
     1141 cccgaggacg gcgcgctcgg caacggcgag gcgccggcgg agacgggcga gcttccggtg
     1201 gtgggcaacg ccgtcggcaa cgaggccctc gccaacgggc aatagcagcc gaaaatccct
     1261 ccccgcgatt ttcgcgggga gggggaccag ccgaaggctg gtggaggggt tgcttcgact
     1321 ccgcgagaac ccctccaccg cgcttcgcgc ggtccccctc cccggcaaat gccggggagg
     1381 aattagaaaa aaaagggccg gggagtcgcc tccccggccc ttcttccgtc tggggcgatc
     1441 ggccggacta gaagtccatt ccgcccattc cgcccatgcc gccgccgggc attccgccgc
     1501 cggccgggga cttgtcctcg ggggcctcgc tgaccgccgc ttcggtggtg atgaggaggc
     1561 cggcgaccga ggccgcgtcc tggagggcgg tgcggacgac cttggtcggg tcgatcacgc
     1621 cggcctggac gaggttctcg tagacctcgg tctgggcgtt gaagccgagc gtctcgtcgt
     1681 cgccgtcgat cagcttgccg gcgacgacgg cgccgtcgtg gcccgcattc tcggcgatct
     1741 ggcggatcgg cgactgcagg gcgcggcgga cgatgtcgat gccgcgggtc tggtcgtcat
     1801 tgactccgga catgcccctg agcgccttgg tggcgtagag cagggcgact ccgccgccgg
     1861 ggacgattcc ttcctcgacc gccgcgcggg tggcgtggag ggcgtcgtcg actcggtcct
     1921 tgcgctcctt gacctcgatc tcggacgagc cgccgacctt gatcacggcg accccgccgg
     1981 cgagcttggc gagacgctcc tggagcttct ccttgtcata gtcgctggtg gtgttctcga
     2041 tctgctggcg gatcgcctcg acccgagcct tgatccggtc gccctcgccg gcgccgtcga
     2101 cgatggtcgt gttgtccttg tcgatggtga cccttttggc ctggcccagc atggcgatgg
     2161 tgacgttctc gagcttgatt ccgagatcct cgctgatcat ctcgccgttg gtcaggatcg
     2221 cgatgtcctc gagcatcgcc ttgcggcgat cgccgaagcc cggggccttg accgccgcga
     2281 ccttgagccc gccgcgaagc ttgttgacga cgagcgtggc cagagcctcg ccctcgatgt
     2341 cctcggcgat gatgagcagc ggacggcccg actgcacgac cgcctccagg atcggaagca
     2401 tcgcctgcag gttggagagc ttcttctcgt ggatgaggat gtagggatcg gtcagctcga
     2461 cctgcatctt ctcaggattg gtgatgaagt agggcgagag gtagccgcgg tcgaactgca
     2521 tgccctcgac gacgtccaat tcgaactcga ggcccttggc ctcctcgacc gtgatcacgc
     2581 cttccttgcc gaccttctcc atcgcctcgg cgatcttctc gccgacgacg gtgtcgccat
     2641 tggcggagat gatgccgacc tgggcgatct cgttggagcc cgagaccggc ttggaccggt
     2701 tcttgagatc ctcgacgacg cgggtgacgg cgaggtcgat gccgcgcttc agatccatcg
     2761 ggttcatgcc cgcggcgacc gacttcatgc cctcgcggac gatcgactgg gcgagaacgg
     2821 tggcggtggt ggtgccgtcg ccggccaggt cgttggtctt gctggccacc tcgcgcagca
     2881 tctgcgcgcc catgttctcg aacttgtcct tgagctcgat ctccttggcg acggtgacgc
     2941 cgtccttggt gatccgaggc gcgccgaagc tcttgtcgag gacgacgttg cggcccttgg
     3001 ggccgagggt caccttcacc gcatcggcga ggatgtcgac gccgcgcagg atccgctccc
     3061 gggcgtcacg cgaaaagcga acgtctttcg ctgccatgtt gattgctcct tcaattcttc
     3121 accccagcga aggctggggg ccatgttgag tcggaatgga tgccagcctc cgctggcatg
     3181 acgggacgcg cggccccgcc gtgagggttc agcccacaat gcccaggatg tcgctttcct
     3241 tcatgatcag caggtcctcg ccgtcgaggc ggacctcggt gccggaccac ttgccgaaca
     3301 ggatgcggtc gccggtcttg acgtcgagcg gcgtgaccgt cccgtcctcg ccgcgcgtgc
     3361 cgctgccggc ggcgacgacc tcgccctcct gcggcttctc cttggcggtg tcggggatga
     3421 tgatgccgcc ggccgtcttc tcctcggcct cgacacggcg gacgagcacc cggtcatgga
     3481 gcggacgaaa attcatgcgc gtcacctctt cttcactgtt gcggcctccg gccccccgcg
     3541 ggcgccgctg ccgacatggt tgaaactgtt agcactcgcc gatggcgagt gccagcagcg
     3601 ctgatctagg cgctgttcat cgcaggtcaa gccttgcgat cgcggaattt tgccctcgcc
     3661 gtgcgccccc gcaatttaca ggggccaggg gctggtctag ccggaatagg gctgttcccc
     3721 gagaagcgga gtgaggcaat gaggttcgcc ggcaaggtct ggaagctgct cgtcggcatc
     3781 aaggacggcc tcgtcctgct gttcatgctg ctcttcttcg gcctgctcta cgcggccatg
     3841 accgcgcgcc cgacgctcgg cgcgggcgat cgcggcgcgc ttctgctcga cctcgacggc
     3901 ccgatcgtcg agcagcccgc ccaggcgacg gcgagcgagg tgttcagcgg cggaggaccg
     3961 cgcgaatacc ggctccgcga cgtcgtccac gcgctcgaca ccgccgccac ggacgaccgg
     4021 gtgaaggcgg tggcgctcaa cctcgacgtc ttctcgggcg gcggacaggc gacgctggcc
     4081 gcggccggcg aggcgttgga ccgggtccga aagaccggca agccggtgct cgccttcgca
     4141 acggcctatg acgacgacag ctaccagctc gccgcccatg ccagcgagct ttggctcgat
     4201 ccgatgggcg gggtgctcgt caccgggccg ggcggcgcca acctgtacat gaagggcctc
     4261 ctcgacaagc tcggggtcac cgccaacgtc tacaaggcgg gcacctacaa ggccgcggtc
     4321 gagcccttca ctcggaccga catgtctccc gaggcgcgcg aggcgctgac cgcggtctac
     4381 ggctcgatgt gggagagctg gcagcaggag gtgcgccagg cccgtcccaa ggcccagctc
     4441 gccccttatc tcgccgacct gcccgggcgc atcgaggcgg ccggcggcga catggcgcga
     4501 gccgcgctcc agtccgggct ggtcgacagg atcggcagcc gcgaggcgtt cgaccgtcgg
     4561 ctcgccgaga tcgccggcac ccgctacgcc gccgttgcgg gaagcttccg ccgaatcccc
     4621 taccggacct ggatcgaggc caacccggcc ggacgcgacg gcggcgatat cggcatcctc
     4681 accgtcgccg gcgacatcgt cgacggcgag gcgggcctgg gcaccgccgg ggcggagacg
     4741 atcgtgcgcg ccctggaaaa gggtcttcgg gagcgcgacc tcaaggcgct ggtgcttcgg
     4801 atcgattcgc cggggggctc cgcgctcgcc tcggagcgga tccggcaggc ggttctggcc
     4861 gccaagtcca aggggctgcc ggtggtggtg tcgatgggca gcctcgccgc ctccggcggc
     4921 tattgggtgg cgaccgcggg cgacaggatc tacgccgagc cgtcgacgat caccggctcg
     4981 atcggcgtgt tcggcatcct gcccagcttc gaggggacga tgaccaagct cggcctcggc
     5041 gccgacgggg tcaagacgac cgcgctgtcg ggcgagcctg acctgctgcg cggcccctcg
     5101 cccgaggccg accggctgct tcagatgggc gtggacggca cctatcgccg cttcctcgcc
     5161 ctggtgtccg cggcgcgcaa gatgccggtc gccgaagtgg accggatcgc ccaggggcga
     5221 atctgggacg gcggcaccgc gcgtcagctg cgcctcgtcg acgcgttcgg cgggctcgac
     5281 gacgcggtcc gggaggccgc gaagcgggcc aagctcgacc ccgacgaggc caaggcggtc
     5341 tggctcgaga agcagcccga tttcagcgac cggctgctga tgtcgctggc cggcgacggc
     5401 gacgaggagg aagccgcgcc ggatgcgttc agccggctcg ccgaccgccc ccgaatcgcc
     5461 gttctgaccg cgatcggcga ggccgagcgg ctgctgaccg gcgccgcgat ccaggcgcgc
     5521 tgcctcgaat gcagcggcca cgccgcatcg cggccgcgcg tcgcaagcgg cggcgccgcc
     5581 gcctggctgg cccgcctgtt cggcctgtga ggctgagagc cgcgacgccc gacgacgccg
     5641 cggccatcgc cgaaatctac gcgccctacg cgacggcgag cgccgtctcg ttcgaaacgg
     5701 agccgcccga cgcg
//
LOCUS       DASVQZ010000270         4457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_535539, whole genome shotgun sequence.
ACCESSION   DASVQZ010000270 DASVQZ010000000
VERSION     DASVQZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535539"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VF547_07365"
     CDS             complement(<1..358)
                     /locus_tag="VF547_07365"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF021908.4"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX8262676.1"
                     /translation="MTATQLDQIPALIEELCTAYDESVAALRSALARYLSKGDRPDPE
                     ARAAGLFAYPELRIHYRDDKPVKFPARAFGRLNQPGTYAISIARPRQFRKYLSEQLHY
                     LVRDYEVEVSVGRSPSE"
     gene            complement(525..932)
                     /locus_tag="VF547_07370"
     CDS             complement(525..932)
                     /locus_tag="VF547_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262677.1"
                     /translation="MTVTPPPPADPAAADALLRTVLAEAASALGRPVDAAGPLAAHLR
                     RLAEDSLATAAALAAGRIGAEAAREAFQGRREALIQIREFAELAALGAAARAADTVFA
                     ALTRTVAARTGVDLAPLFGAPAPDGGLSPPRSG"
     gene            complement(945..1394)
                     /locus_tag="VF547_07375"
     CDS             complement(945..1394)
                     /locus_tag="VF547_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262678.1"
                     /translation="MKMHRTMLAALAAALLLPPAGCQAPPLDPRIGAATRAAHEGAMQ
                     LVAEAEAGRFADPASFEGAAPRYAALDAQLAEARARAEAAPPGAGPLPRRAAALLPRQ
                     IEGCRSQVERLAGIHRRDGIGPGAGLTGSVAVACDLARRAVDPSAAR"
     gene            complement(1441..2532)
                     /locus_tag="VF547_07380"
     CDS             complement(1441..2532)
                     /locus_tag="VF547_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262679.1"
                     /translation="MKQEHHESPPSEEAAADPFPRFDEEGHAPPAARAEAAPASTAPA
                     RIGWIDGHMPDIVDPLEPLHPETAFTQTRRLRHDGWSPEKMRLFLERFAECGVVVEAC
                     HAAGMSARAAYNLRDRDPLFAAGWDAAAVKARTPLADEAYSRARNGVVERIYRDGVVV
                     AERHRYDNRLTMAVLTRLDSRVDRAEAQGAPHLAVAARWDDYLAALGEDRREDGMALL
                     VPQAADPAPAKRAMESLQDRALREFQLQAEAEPDEDPHSLWDEEDGWWTDYPPPPGFE
                     GVEEGSYGDQDYRRSLSPEEQAVIDADLAQEEAEGAEEDAEALAIAAARRDAFFGFSP
                     EPRSEAEEVGDDDDGEDDGPGDVEDDGAD"
     gene            complement(2692..3222)
                     /locus_tag="VF547_07385"
     CDS             complement(2692..3222)
                     /locus_tag="VF547_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262680.1"
                     /translation="MSLSDVQSLLDSARSYARSDPPVSIWKAGLAAEAIAAEVGAAAG
                     RAKRAEQSHELYLEELERAGLIAFRDARQFHGVRVLRNRAVHGHEGSTADAQRALRLA
                     DGLAGLAEAKGAGRAAAGFGTKGAGGRASASGRTGAGGAGINWVAWLLLLSGLALLGL
                     FLLDPDRFLAALRDLF"
     gene            complement(3350..3571)
                     /locus_tag="VF547_07390"
     CDS             complement(3350..3571)
                     /locus_tag="VF547_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262681.1"
                     /translation="MTALEGTSDDDAFNAAADAHDRALERLLLAPAPSVAALAAKLRL
                     ARRDQAWELPSGNALMGALEQDALRLAGA"
     gene            4164..>4457
                     /locus_tag="VF547_07395"
     CDS             4164..>4457
                     /locus_tag="VF547_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8262682.1"
                     /translation="MDSDLVGINEIAELAGVSRQAVANWRVRFRDFPRPVADLKAGPV
                     FDLDQVKVWLRRRKIPMARVVALINLKGGVAKTTTTVALAETLAAQYRKKVLVI"
BASE COUNT          623 a         1450 c         1699 g          685 t
ORIGIN      
        1 tctcgctcgg cgagcggccg accgatacct ccacctcata gtcgcggacc agataatgga
       61 gctgctcgga aaggtatttg cggaactggc gcggccgggc gatcgagatc gcataggtgc
      121 cgggctggtt gagccgcccg aaggcgcggg ccgggaactt gaccggcttg tcgtcgcgat
      181 agtggatgcg cagctcggga taggcgaaca ggcccgccgc ccgcgcctcc gggtccggcc
      241 ggtccccctt cgaaaggtag cgggcgagcg cgctgcgcag cgccgcgacc gactcgtcgt
      301 aggcggtgca aagctcctcg atgagagcgg gaatctggtc gagctgggtg gcggtcatgc
      361 cgcgcctctt ggcacgggat cgccgccgaa gcgagagggg tgcgcgggcg ccgatgcgcc
      421 tcgggcgcct gaggaggctg tgccgccccc cgctgcgttc ggcctgatct tgtcgaaggc
      481 ctcccttccc ttgcggctga agaggaggga gggcttcgac aggctcagcc cgaacggggt
      541 gggctgaggc caccatccgg cgccggtgcg ccgaaaagcg gcgcgagatc tacgccggtg
      601 cgcgccgcga cggtgcgggt cagcgctgcg aagacggtgt cggcggcgcg ggcggcggcg
      661 ccgagggcgg cgagctcggc gaattcgcgg atctggatca gcgcctcgcg gcgtccctgg
      721 aaggcctcgc gcgcggcttc ggcgccgatg cggccggcgg cgagcgcggc ggcggtggcg
      781 aggctgtcct cggcgaggcg gcgaaggtgg gctgcgagcg gcccggcggc gtcgacgggg
      841 cggccgagcg ccgaagcggc ttcggcgagg acggttcgaa ggagagcgtc ggcggcggcc
      901 gggtcggcgg gtggaggcgg cgtgacggtc atggtcctgt ccttctatcg agcggccgac
      961 ggatcgacgg cgcggcgggc gaggtcgcag gcgacggcga cgctgccggt gagccccgcg
     1021 cccggcccga tgccgtcgcg gcggtggatg ccggcgagcc gctcgacctg gctgcggcac
     1081 ccttcgatct ggcgcggcag cagggcggcg gcgcggcggg ggagcggacc ggcgccgggc
     1141 ggcgcggcct cggcccgcgc gcgcgcttcg gcgagctgcg catcgagcgc ggcgtagcgc
     1201 ggcgcggcgc cttcgaagct cgcgggatcg gcgaaccgcc ccgcctccgc ttcggcgacg
     1261 agctgcatcg cgccctcatg ggccgcgcga gtcgcggcgc cgatccgcgg atcgagcggc
     1321 ggcgcctggc agcccgccgg cggcagcagc aaggcggcgg cgagcgccgc gagcatggtc
     1381 ctgtgcatct tcatgccggt ctccgttcca aaatctctgc gtggcggcgg cggcgggcag
     1441 ctagtccgcg ccgtcgtcct cgacgtcccc cgggccgtcg tcctcgccgt catcgtcgtc
     1501 tccgacttct tcggcctcgc tcctgggttc ggggctgaag ccgaagaagg cgtcgcggcg
     1561 ggctgcggcg atcgccagtg cctcggcgtc ctcctcggcc ccctccgctt cctcttgggc
     1621 gaggtcggcg tcgatcaccg cctgctcctc gggcgagagc gagcggcgat agtcctgatc
     1681 gccgtagctc ccctcctcca cgccttcgaa gccgggcggg ggcgggtaat cggtccacca
     1741 gccgtcctct tcgtcccaaa ggctgtgggg atcctcgtcg ggctccgcct ccgcctggag
     1801 ctgaaattca cgaagtgcac ggtcctgcaa actttccatc gcgcgcttgg cgggcgccgg
     1861 atcggcggcc tgcggcacga gcagcgccat gccgtcctcg cgccggtcct cgccgagcgc
     1921 ggcgagataa tcgtcccagc gcgcggccac ggcgaggtgc ggcgcgccct gcgcttcggc
     1981 gcggtcgacc cggctgtcga ggcgggtgag gacggccatg gtgaggcggt tgtcgtagcg
     2041 gtggcgctcg gcgacgacga cgccgtcgcg gtagatccgc tcgacgaccc cgttgcgggc
     2101 gcgcgaatag gcctcgtcgg ccagcggcgt ccgcgccttg acggcggccg cgtcccagcc
     2161 cgcggcgaag agcggatcgc ggtcgcgcag attgtaggcc gcgcgcgcgc tcatcccggc
     2221 cgcatggcac gcctccacga ccacgccgca ctcggcgaac cgctccagga agaggcgcat
     2281 cttctccggc gaccagccgt cgtggcggag gcggcgggtc tgggtgaagg cggtctcggg
     2341 gtgaagcggc tcgagcggat cgacgatgtc tggcatgtgg ccgtcgatcc agccgatgcg
     2401 ggcgggggcg gtggaggccg gcgcggcttc ggcccgggcg gcgggcggag cgtggccctc
     2461 ctcgtcgaag cgcgggaagg gatcggcggc ggcctcttcc gagggaggcg actcgtgatg
     2521 ttcctgtttc attccacagg ttagatcagg tgatttccta ttttgcaata gtgccggttg
     2581 ggttcctttt tcctagggcg ccgcatgaag ggaaggcagg cgggctcacg caaagccgca
     2641 aagaacgcaa agtcgcaaag ggttgccgag gcgggcggcg gcggcggcca attaaaagag
     2701 gtcgcgcaga gcagcgagga agcggtccgg atcgaggagg aagaggccga ggagggcaag
     2761 gccggagagg aggaggagcc aggcgaccca gttaatgccg gcgccgcctg cgcccgtcct
     2821 gccggaggcg gaggcgcggc cgccggcgcc ctttgtgccg aagccggccg ccgccctgcc
     2881 cgcgcccttg gcctcggcaa ggcccgcgag cccgtcggct aggcggagcg cgcgctgggc
     2941 gtcggcggtg ctgccttcgt ggccgtggac ggcgcggttg cgcagcacgc gcacgccgtg
     3001 gaactgccgc gcgtcccgga aggcgatgag gccggcgcgc tccagctcct cgaggtagag
     3061 ctcgtggctc tgctcggccc gcttcgctcg cccggccgcc gccccgacct ccgccgcgat
     3121 cgcctcggcc gccagccccg ccttccagat cgaaaccggc ggatcgctgc gcgcatagga
     3181 acgcgcgctg tcgagcaggc tttgcacgtc gctgaggctc atggggtgga ggtccggagt
     3241 tggggtttgc gagcgccgga ggcggcgcgg ccccggtcgg gagcgggttt ccccgaggcg
     3301 ggcgcagggt tcaaccgtta tttggaggcg cgctccgtgg agcgcagcgt cacgcgccgg
     3361 cgaggcggag ggcgtcctgt tcgagcgcgc ccatgagcgc attgccggag gggagctccc
     3421 aggcctggtc gcggcgggcg aggcggagct tggcggcgag ggcggcgacg ctcggcgcgg
     3481 gggcgaggag gaggcgttcg agagcgcggt cgtgcgcgtc ggcggcggcg ttgaaggcgt
     3541 cgtcgtcgga cgtgccttcg agcgccgtca cggccgcatc ggcgcggttg aagacggcga
     3601 gggcttcttt ccacagctcg tcaccccgga cttgatccgg ggtccacctg ccttcttccg
     3661 gcgcgggtaa gaaggtggat tccggatcaa gtccggaatg acggccttgg actcggctca
     3721 gcatcggcgc ggaggcgcac gcaacgccta gcagcgcccg gcgcgacagt ttaagctgcc
     3781 catcagccat cattcgcctc ctcgaacggg gcgtttgcgg gtcggagccg aagcgaaggg
     3841 ctgcggcttc tgtttgtggt gttaccatat attaggggcg ggtcaatagc cgacgttgca
     3901 aaaagacccc gcggccgccc gcgcaccggc agggacccgg tgcgaaccgt ccgctggtcc
     3961 gacgaattcg aggccgcgat tgctgcatgg ggggagcggc aacccgagcc caagccatcg
     4021 cgatccgaag ccattcgcct cctgatcctg aagggtttat ccgctatgga gtgatttacc
     4081 tccagatcgg tgtgaacggt ggtcacagaa ctcttgcgcg atgacacatc ggtgtgtaca
     4141 tacaactcaa cctgaggatt gcagtggatt cagatttagt cggcattaac gagattgcgg
     4201 aattggcagg ggtatctcgc caagcggttg cgaactggcg cgtccggttc cgcgacttcc
     4261 ctcgtccagt ggccgatctg aaagctggcc ctgtcttcga ccttgatcag gttaaagtat
     4321 ggctcagacg gaggaaaatt cccatggctc gcgttgttgc acttatcaac ctcaagggcg
     4381 gtgtcgccaa gaccaccacg acggttgctc tcgccgaaac tctcgcggca caatatcgta
     4441 aaaaagttct ggtgatt
//
LOCUS       DASVQZ010000271         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_251271, whole genome shotgun sequence.
ACCESSION   DASVQZ010000271 DASVQZ010000000
VERSION     DASVQZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251271"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF547_07400"
     CDS             complement(<1..308)
                     /locus_tag="VF547_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8262683.1"
                     /translation="MRTDLLLLTPLARLADLALLLLRWVTGAFLVWQSHDNIFSTARM
                     EEFEKFLVQFGFPLPALMAPLCVWAQFLCGFAFILGLMTRWAGLVAAFVVVVAVWMVH
                     "
     gene            complement(262..1569)
                     /locus_tag="VF547_07405"
     CDS             complement(262..1569)
                     /locus_tag="VF547_07405"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0071513 - phosphopantothenoylcysteine
                     decarboxylase complex [Evidence IEA];
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase"
                     /protein_id="HEX8262684.1"
                     /translation="MNGQRILLIVGGGIAAYKACELVRLIRKSGGSVRCVLTAGGAQF
                     VTPMTLAALSEQPVHTSLWDLKDEVEMGHIQLSREADLVVVCPATADLMAKMAAGIAD
                     DLATTLLLATDKPVLAAPAMNVRMWQHEATKRNVARLRADGVAILEPDEGEMACGEYG
                     PGRLPEPEAILAAITKELSVRAEPVEALPSSGPGEGKEWPSTGSGRTEVLAGRHIIVT
                     AGPTHEPIDPVRLIANRSSGKQGFAIAEALAELGARVTLVAGPVVLPTPTGVERVDVE
                     TAREMEAAVVAALPADAAVLVAAVADWRVDPAPSKLKKSAGPPDLVWIANPDVLAGLA
                     TGPHRPRLLVGFAAETGDVVNVAQAKRASKGCDWIVANDVSGDVMGGDRNRVHLITAD
                     GVDSWEEAGKDEVARRLAERIAAALGDEDANGPSASDPAGEAG"
     gene            complement(1566..1919)
                     /locus_tag="VF547_07410"
     CDS             complement(1566..1919)
                     /locus_tag="VF547_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX8262685.1"
                     /translation="MAPGPEADGLYLERILELIESIENSLAEVDQASFLASRDKVDVT
                     AFRLFQIGELTNKLSEGPKQRHPEIAWPSIYGLRNLIAHHYDKTNPTALWTIAANSLS
                     TLRTVCQHELDHLDG"
     gene            complement(1919..2212)
                     /locus_tag="VF547_07415"
     CDS             complement(1919..2212)
                     /locus_tag="VF547_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8262686.1"
                     /translation="MDRDQVIRILRENADELHGMGLAKLYLFGSVARGESKPTDIDLA
                     FEIGGNPHFSLLTQAGATVRIEEMLGRPVDLVQRECLHPRIKERVESEMVEVF"
     gene            complement(2218..3750)
                     /gene="ubiB"
                     /locus_tag="VF547_07420"
     CDS             complement(2218..3750)
                     /gene="ubiB"
                     /locus_tag="VF547_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX8262687.1"
                     /translation="MTRPVVHIWRLLKWGRVLARHGALRGIERDSNTPQPVRRLARIA
                     RFGARVPRVPTYGEAFQELGPAAIKFGQALATRPDLVGVEAARDLQLLQDSLPPAPFE
                     DVRHAIEQGLEKPLEEIFADIDPEPVGAASIAQVHRAHTTDGKLVAVKVLRPNIEDDF
                     ARALDTYAWAAAQVEQMGGEAARLRPRAVIAYFRQWTARELDLRREAASASELRENLI
                     AESGFYVPAIDWTRTSRRVLTLEWIDGIKLNDRDALVAAGHDLKALAATLVRAFLRQA
                     VVDGYFHADLHQGNLFALPDGRLAAIDFGIMGRIDRQARVWLAEILYGLLTGNYRRVA
                     EIHFEAQYVPAHHDVDEFATALRAVGEPIRGLPVKDISIGQMLDGLFTITRDFDMPTQ
                     PHLLLLQKTMVMEEGVAHALDPDINMWETAEPFLREWVRGELGPEAALADRLVRDFRT
                     FAKIPELIRRIDYYYPPPGAAPPDPPLADVVVVKPRRYWRYALTAIVSAAAGVGGALL
                     LF"
     gene            complement(3747..4475)
                     /locus_tag="VF547_07425"
     CDS             complement(3747..4475)
                     /locus_tag="VF547_07425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8262688.1"
                     /translation="MDKVSFGFEEVSPEEKTRRVGAVFSSVARRYDLMNDLMSAGMHR
                     PWKDLFVRRVRPRRGEAILDMAGGTGDVAFRLARRGARVTVADINPDMLEVGRERAAK
                     RGVEGLAWAVENAEALSFPDAAFDAYTIAFGIRNVTDVDAALREAHRVLRTGGRFFCL
                     EFSTTRWPGFAGLYDAYSMHAVPRIGKTVAGDEESYRYLVESIRRFPPMEKFSATIAQ
                     AGFAQVRAEPILGGLVAIHSGWKI"
BASE COUNT          712 a         1551 c         1560 g          720 t
ORIGIN      
        1 cagtgcacca tccagaccgc gacgacgacg acgaaggcgg cgacgagccc ggcccagcgg
       61 gtcatcaggc cgaggatgaa ggcgaatccg cacaggaact gcgcccagac gcagagcggg
      121 gccatcaggg cgggcagggg gaagccgaac tggacgagga acttctcgaa ctcctccatc
      181 cgggccgtgc tgaagatgtt gtcgtggctc tgccagacca ggaaggcgcc ggtcacccag
      241 cgcagcagga gcagggcgag atcagccagc ctcgccagcg gggtcagaag cagaaggtcc
      301 gttcgcatcc tcgtccccaa gagcggccgc gatccgttcg gcgaggcgcc gcgcgacctc
      361 gtccttgccc gcttcctccc agctgtcgac gccgtcggcg gtgatcaggt ggacgcggtt
      421 gcggtcgccc cccatcacgt ccccggatac atcattggcg acgatccagt cgcacccctt
      481 gcttgctcgt ttcgcctgcg caacgttcac gacgtcgccg gtctccgcgg cgaagccgac
      541 caggaggcgc ggccgatggg ggcctgtggc gaggccggcg aggacgtcgg ggttcgcgat
      601 ccagacgagg tccggcggcc ccgccgattt cttcagcttc gagggggcag ggtcgacccg
      661 ccagtcggcg accgccgcca ccagcacggc ggcgtcggcg ggaagggcgg cgacgacggc
      721 ggcctccatc tcgcgagccg tctcgacgtc gacccgctcg acccccgtcg gcgtcggaag
      781 gacgaccggg ccggccacca atgtcacgcg cgctcccagc tccgccagcg cttcggcgat
      841 ggcgaagccc tgcttgcccg atgaccggtt cgcgatcagc cgaaccgggt cgatcggctc
      901 gtgcgtcggc cccgcggtga cgattatgtg ccgaccggcc aacacctccg ttcggcctga
      961 gcctgtcgaa ggccattcct tgccttcacc cgggccggac gagggaaggg cttcgacagg
     1021 ctcagcccga acggagagtt ctttcgtgat cgctgcgagg atcgcttccg gctccggcag
     1081 ccgtcccggc ccatattcgc cgcaggccat ctcgccttcg tccggctcca ggatcgcgac
     1141 gccgtccgca cggagccgcg cgacattgcg cttcgtcgcc tcgtgctgcc acatcctgac
     1201 gttcatcgcc ggcgccgcca gcaccggctt gtcggtggcg aggaggaggg tggtggcgag
     1261 gtcgtccgcg atgccggccg ccatcttcgc catcaggtcg gccgtcgccg ggcagacgac
     1321 gaccaggtcg gcctcgcggc tcagctggat atgccccatc tcgacctcgt ccttgaggtc
     1381 ccacaggctg gtatggaccg gctgctcgga gagggcggcc agggtcatcg gagtgacgaa
     1441 ctgcgccccg cccgcggtca gaacgcagcg cacgctccct cccgacttgc ggatcagccg
     1501 caccagctcg caggccttgt aggccgcgat cccgccaccg acaatgagca ggatgcgctg
     1561 gccgttcatc cgtcgaggtg gtccagctcg tgttggcaga ccgtccgaag ggtactcaag
     1621 ctgtttgccg cgatcgtcca gagcgcggtg gggttcgtct tgtcgtaatg atgcgcaatc
     1681 aggttccgaa gcccatagat cgacggccag gcgatctcgg ggtggcgttg ctttgggcct
     1741 tcggacagct tgttggtcag ttcgccgatc tggaagagcc gaaatgccgt gacgtcgacc
     1801 ttgtcccggc tggctagaaa gctcgcctga tcgacttcgg caagactgtt ctcaatggat
     1861 tcgatcagtt cgaggatgcg ctccagatag aggccgtccg cttcgggtcc cggcgccatc
     1921 agaacacctc caccatctcg gactcgacgc gctccttgat cctggggtgc agacattccc
     1981 gctgtacgag atcgacaggc cggccgagca tctcctcgat ccgaaccgtt gcgccggcct
     2041 gtgttaacag actgaagtgc ggattgccgc cgatctcaaa agccaggtcg atgtccgtcg
     2101 gcttcgattc gccgcgcgcc acggagccga acaggtagag cttcgccaag cccattccat
     2161 gaagctcgtc ggcattctca cgtagaatgc gaatgacttg gtcgcggtcc atgtccccta
     2221 aaataagagc agtgcgccgc ccacgccagc cgccgccgaa acgattgcgg tcagcgcata
     2281 gcgccagtag cgccgcggct tcaccacgac gacgtcggcg agcggcggat cgggcggcgc
     2341 ggcgccgggg ggcgggtagt aatagtcgat ccggcggatc agctcgggga tcttggcgaa
     2401 ggtcctgaag tcgcggacga ggcggtcggc gagcgccgcc tcgggcccga gctcgccgcg
     2461 cacccactcg cgcaggaacg gctcggccgt ctcccacatg ttgatgtcgg gatcgagggc
     2521 gtgcgcgacg ccttcctcca tcaccatcgt cttctggagc agcagcagat ggggctgggt
     2581 cggcatgtcg aagtcgcggg tgatggtgaa cagcccgtcg agcatctggc cgatcgagat
     2641 gtccttgacc ggcaggccgc ggatcggctc gccgaccgcc cgaagcgcgg tcgcgaactc
     2701 gtcgacgtcg tggtgcgccg gcacatattg ggcctcgaaa tggatctcgg cgacccggcg
     2761 gtagttgccg gtgagcaggc cgtagaggat ctcggccagc cagaccctgg cctggcggtc
     2821 gatccgtccc atgatcccga aatcgatcgc ggcgaggcgg ccgtcgggaa gggcgaacag
     2881 attgccctgg tggaggtcgg catggaaata gccgtcgacc acggcctggc gcaggaaggc
     2941 gcgcacgagg gtcgccgcca gcgccttgag gtcgtgcccc gccgccacca gcgcgtcgcg
     3001 gtcgttgagc ttgatgccgt cgatccattc gagcgtcagc acccggcgcg aggtccgggt
     3061 ccagtcgatc gcgggcacgt agaagccgct ttcggcgatc aggttctcgc gcagctcgga
     3121 agcggaggcc gcctcgcggc gaaggtcgag ctcgcgcgcc gtccattgcc ggaaataggc
     3181 gatgacggcg cgcggcctga gcctcgccgc ctcgccgccc atctgctcca cctgggccgc
     3241 cgcccaggcg taggtgtcga gcgctcgcgc gaaatcgtcc tcgatgttgg gcctgagcac
     3301 cttgaccgcg accagcttgc cgtcggtggt atgggcgcgg tggacctggg cgatcgaggc
     3361 ggcgccgacc ggctccgggt cgatgtcggc gaaaatctcc tcgagcggct tctccagccc
     3421 ctgctcgatg gcgtgccgga cgtcctcgaa cggcgcgggc ggaaggctgt cctggagcag
     3481 ctggaggtcg cgggccgcct cgactccgac caggtcgggc cgagtcgcca gcgcctggcc
     3541 gaacttgatc gccgccggtc ccagctcctg gaaggcctcg ccataggtcg gcactcgagg
     3601 cacccgcgct ccgaagcggg cgatccgggc gaggcggcgg accggctggg gcgtgttgga
     3661 gtcgcgctcg atgccgcgca gcgctccgtg ccgcgccagc acccgccccc atttgagaag
     3721 gcgccagata tggacgacgg gacgggtcaa atcttccagc cgctgtggat ggcgacgagg
     3781 ccgccgagga tcggttcggc cctgacctgg gcgaagcccg cctgcgcgat cgtcgccgag
     3841 aatttctcca tcggcgggaa gcggcggatc gattcgacca ggtagcggta gctctcctcg
     3901 tcgcccgcca ccgtcttgcc gatccgcggc acggcatgca tcgaataggc gtcgtagagg
     3961 ccggcgaagc ccggccagcg ggtcgtcgag aattcgaggc agaagaagcg gccgccggtc
     4021 ctcagcacgc ggtgggcctc gcgcagcgcc gcgtcgacgt cggtcacgtt tcggatgccg
     4081 aaggcgatcg tgtaggcgtc gaaggcggcg tcggggaagc tcagcgcctc ggcattctcg
     4141 accgcccagg cgaggccctc gacgccgcgc ttggccgccc gctcgcggcc gacctcgagc
     4201 atgtcgggat tgatgtcggc gaccgtcacc cgagcgccgc ggcgcgccag ccggaaggcg
     4261 acgtcgccgg tgccgccggc catgtcgagg atcgcctcgc ccctgcgggg ccgcacgcgg
     4321 cggacgaaca ggtccttcca cggccggtgc atccccgccg acatgaggtc gttcatcagg
     4381 tcgtagcggc gggcgacgct tgagaagacg gcgccgacgc gccgggtctt ctcctcgggc
     4441 gaaacctcct cgaagccgaa agaaaccttg tccatcgcgc ccgcctctag caggggtttc
     4501 ggcgcatgtg cactcgccat cttgtcctct ccgtcatccc agc
//
LOCUS       DASVQZ010000272         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_251763, whole genome shotgun sequence.
ACCESSION   DASVQZ010000272 DASVQZ010000000
VERSION     DASVQZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251763"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VF547_07430"
     CDS             <1..774
                     /locus_tag="VF547_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262689.1"
                     /translation="GSRVANRLYVYTAGHGFAPDPRSRSLILADALGTAKVPNFHAPA
                     WIDWFASQYHFEELVLWMDCCATQTFDYAPGVPPMPRTAARGGGDPAKVFMAFGSGVG
                     RAAYEGPIGPHKEVRGLFTDRLLRGLRGAAANENGEVRSASLKKFLYNGSAASTDAPR
                     EHPVAEPVIPQEDDMLFAVVPHAQRPVYSIKAGAADGTPLPEGTRIDLLNGNFAKIGE
                     APVVGGRVSFQLSLGLYKLRGPGWERFLDIGAGTPFRID"
     gene            777..2210
                     /locus_tag="VF547_07435"
     CDS             777..2210
                     /locus_tag="VF547_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262690.1"
                     /translation="MPTLTINTHSDAEVFIIDASLELIDYHVGATSRDLDSGLYKIKV
                     DRGGAIREQLVALESDDAELDLRIDHFPAVAPIGPMLGADAEAVQSLVREALDKAEQR
                     WPGADLIVLLCHWASGRGDPGRPFAGIRLFPWRASGLAFNVVEAPSVFARLGELDWAA
                     AAVPVAAGKREYILEMKDRSRTSRQVLLTAPGWQTRIFLRRRPAPSVTGHKDPCEARD
                     TIDVSIQMGRRGAPCVYCDHYETIEVSRNILAKGRALATPELILSLLHGKFDNPIHGI
                     VGLHLMLGLLEYAKLHPDSDMGADHLRTTTGREPSDYVAMVLRNLSDVLAVRDDEDLP
                     ADLVALQARAGFATDRHFRVKAPPMFWASWDALRSNAVENGCVSIGRDLWQSLGDGAP
                     WGPYLAWSPRRDGLERRLDALLQRLAERETPAARSLRKILKPSRDLAAALGIPYSVSH
                     SLRAGGKRRGAKKGGRQRPAGKGAGRV"
     gene            complement(2217..>3116)
                     /locus_tag="VF547_07440"
     CDS             complement(2217..>3116)
                     /locus_tag="VF547_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX8262691.1"
                     /translation="QMPEIIERGHLFIAQPPLYKVAKGRSEVYLKDDSKLDDYLVEAG
                     IGLMRLETAEGPRSGEDLKHLADHARRVRSLMRYVPRRYDPGLIEALALNGALDPALP
                     PARRAEAIGRAAAFLQAGDDGSAWTGEVGADGDYVLRRLWRGVTDHHVVDHKFVASAE
                     ARKLHGLAAEQAATYASASRLVSVKAESAESEEGDSEASDEPALPSKGALVRRPTELL
                     EAILAAGRKGLAVQRYKGLGEMNADQLWETTLDPDNRSLLRVEIAQADVADEIFTRLM
                     GDVVEPRREFIQENALNVANLDV"
BASE COUNT          455 a         1105 c         1082 g          474 t
ORIGIN      
        1 ggctcgcggg tcgccaaccg tctctacgtc tataccgccg ggcacggctt cgccccggat
       61 ccgcgctcgc gctcgctcat cctcgccgac gccctgggaa cggccaaggt ccccaacttc
      121 cacgcgcccg cctggatcga ctggttcgcc tcccaatatc atttcgagga gctggtgctg
      181 tggatggact gctgcgccac gcagaccttc gactacgcgc cgggagtccc gccgatgccg
      241 aggacggcgg cgcgcggcgg cggcgacccg gcgaaggtct tcatggcctt cggctcagga
      301 gtcggccgag ccgcctatga gggtccgatc ggaccccata aggaggtgcg gggactgttc
      361 accgaccgcc tgcttcgcgg cctgcgcgga gccgccgcca acgagaatgg cgaggtccgc
      421 tccgcgtcgc tgaagaagtt cctctacaac ggcagcgccg cttcgaccga tgcgccgcgc
      481 gagcaccccg tcgccgagcc cgtcatcccg caggaggacg acatgctgtt cgccgtcgtc
      541 ccccacgcgc agcggccggt ctacagcatc aaggccggcg cggccgacgg aacgcctctc
      601 cccgaaggca cccgcatcga cctgctcaac ggcaatttcg cgaaaatcgg cgaggcgccc
      661 gtcgtcggcg gccgcgtatc gttccagctc tcgctcggcc tctacaagct cagggggccg
      721 ggatgggagc ggtttctcga catcggggcc gggaccccgt tcaggatcga ctgacgatgc
      781 cgactctgac catcaacacg cattccgacg ccgaggtgtt catcatcgat gcgtcgctcg
      841 agctgatcga ctaccatgtc ggcgccacca gtcgcgacct cgacagcggg ctgtacaaga
      901 tcaaggtcga ccgcggagga gcgatcaggg agcagctcgt cgcgctggaa agcgacgacg
      961 ccgagctcga cctgcggatc gaccatttcc cggcggtggc gccgatcggg ccgatgctcg
     1021 gcgccgacgc cgaggccgtc cagagcctgg tccgcgaagc gctggacaag gccgagcagc
     1081 gctggccggg agcggacctc atcgtcctgc tgtgccactg ggcctcgggg cgtggcgatc
     1141 cggggcggcc cttcgccggg attcggctgt ttccttggcg ggccagcggg ctggcgttca
     1201 acgtcgtcga ggcgccgagc gtcttcgcgc ggctcggcga gctcgactgg gcggcggcgg
     1261 ccgtccccgt cgcggccggc aagcgggaat atatactcga gatgaaggac cggtcgcgga
     1321 cctctcggca ggtgctcctc accgctcccg gctggcagac ccggatcttc ctccgccgcc
     1381 gtccggcgcc gtcggtgacc ggccataagg acccctgcga ggccagggat acgatcgacg
     1441 tctcgatcca gatgggccgt cgcggcgcac cctgcgtcta ttgcgaccat tatgagacga
     1501 tcgaggtctc ccgcaacatc ctcgccaagg gccgggcgct ggcgactccc gagctgatcc
     1561 tgagccttct ccacggcaag ttcgacaatc cgatccacgg catcgtcggg ctgcatctga
     1621 tgctcggcct tctggaatat gccaagctcc atcccgattc cgacatgggc gcggaccacc
     1681 tgcgaacgac gaccggccgc gagccgagcg actatgtcgc catggtgctg cgcaacctca
     1741 gcgacgtcct cgcggtccgc gacgacgagg atctgccggc cgacctggtg gcgcttcagg
     1801 cccgcgccgg attcgccacc gatcggcact tccgcgtgaa agcgccgccg atgttctggg
     1861 ccagctggga cgcgcttcgc tcgaacgccg tggagaacgg ctgcgtctcg atcggccgcg
     1921 acttgtggca gagcctcggc gacggggcgc cctggggccc ctatctggcc tggtcgcccc
     1981 gccgcgacgg cctcgagcgc cgcctcgacg ccttgcttca gcgcctcgcc gagcgggaga
     2041 cgccggcggc caggtcgctg cgcaagatcc tcaagccgag tcgggacctg gcggcggcgc
     2101 tgggcatacc ctattcggtc agccactcgc tgcgcgccgg cggcaagcgg cgcggcgcga
     2161 agaagggcgg tcggcagcgg ccggccggga aaggcgcggg ccgggtctga cggccctcag
     2221 acgtcgagat tggcgacgtt gagcgcattc tcctggatga actcgcgccg aggctcgacg
     2281 acgtcgccca tcagccgggt gaagatctcg tcggcgacgt ccgcctgggc gatctcgacc
     2341 ctgagcagcg agcggttgtc ggggtcgagc gtggtctccc agagctggtc ggcgttcatc
     2401 tcgccgaggc ccttatagcg ctggacggcg aggcccttgc ggccggcggc gaggatggcc
     2461 tcgagcaatt cggtcggccg gcgcaccagc gcgcccttgg agggcagcgc cggctcgtcc
     2521 gacgcctcgg aatccccctc ctccgactcg gccgactccg ccttcaccga caccagccgc
     2581 gaggcgctgg cataggtcgc ggcctgctcg gcggcgaggc cgtgaagctt gcgcgcctcg
     2641 gccgaggcga cgaacttgtg gtcgacgaca tgatggtcgg tgacgccgcg ccacagccgg
     2701 cggaggacgt agtcgccgtc ggcgccgacc tcgcccgtcc aggccgagcc gtcgtcgccc
     2761 gcctgcagga aggcggcggc gcggccgatc gcctcggcgc gccgggcggg cggcagggcc
     2821 gggtcgagcg ccccgttgag cgcgagcgcc tcgatcaggc cgggatcgta gcggcgcggc
     2881 acgtagcgca tcaggctgcg cacccggcgg gcatgatcgg cgaggtgctt gagatcctcg
     2941 ccgctgcgcg gcccctcggc cgtctcgagc cgcatcaggc cgatgcccgc ctcgaccagg
     3001 taatcgtcga gcttggaatc gtccttgaga tagacttcgg agcggccctt ggcgaccttg
     3061 tagagcggcg gctgggcgat gaagaggtgc ccgcgctcga tgatctcggg catctg
//
LOCUS       DASVQZ010000273         6807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_922508, whole genome shotgun sequence.
ACCESSION   DASVQZ010000273 DASVQZ010000000
VERSION     DASVQZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922508"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1660
                     /locus_tag="VF547_07445"
     CDS             161..1660
                     /locus_tag="VF547_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8262692.1"
                     /translation="MAKAATKPKLGFAQLWNISFGFFGIQIGFALQNANMSRIFQSLG
                     TSLDDLPALWVAAPLTGLLVQPVIGHMSDRTWLGRLGRRRPYFLGGAILAALSLFVMP
                     LAPALLFAAVMLWVLDASLNVSMEPFRAFVGDMLRKDQHSAGYAVQTAFIGAGAVVGS
                     IVPWALEQIGVANVAPDGGIPDTVRYAFWFGGAALFLAVLWTVLTTREYSPEQMAEFE
                     GARTEEEGQVVRMLAARSFASSLAWIAAGALIVFAVRQLGLEKEVYLLGGLLIAYGLA
                     SIVAIRGARGGAAPGMLGHIVGDFSGMPELMKRLALAQFFSWSALFIMWINTTPVVAQ
                     YFYGSADAASPAYQQGANWVGVLFTAYNGVAAIAALTLLPFLARRIGRVRTHFAALLC
                     GAAGYASFFFLDDPRLLIASEVGVGIAWASILAMPYAILASALPQRKLGIYMGLFNVF
                     VVVPQLLVATVMGSIMKRFFPGEPIWTMAFAAGVMILAALAMLRVEEPK"
     gene            1756..3141
                     /locus_tag="VF547_07450"
     CDS             1756..3141
                     /locus_tag="VF547_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8262693.1"
                     /translation="MRSPSPFRGGIWALLLLLFIFATPAVSADRYDAVDLSGVKHPDW
                     AKDAVLYQLNTRQFTPEGTFRAAERQLPRLKALGADIVWLMPVHPIGEKNRKGTLGSP
                     YSVRDYYAVNPEFGTMDDLKSFVREAHRLDMRVILDWVANHSAWDNPLVAQHPDWYDR
                     DWKGDFRPTPWWDWSDIIDFDYRKTGIRRYMAGAMRFWVSEADVDGFRADVAAYMPLD
                     FWERVRADLDEVKPVFMLAEAQMRDLHYKAFDASYAWGWYGAVSDIAKGKADVGALFG
                     YYSENESAWPAGAMRMTYVENHDQNAWHGTQFEQFGPALTNAIVLSVAGEGIPLIYNG
                     QEAGNPKRLAFFERDPIAWREHPIGDLYKRLFALKQANRALWNAPWGGRMVGVVNSAP
                     AKVFSFVRRTRSDRVFAVFNFSPEPQRVTFGEGPHRGRFRDFDTGRQITIDASTELAL
                     APWSYRLMSIE"
     gene            3138..4652
                     /locus_tag="VF547_07455"
     CDS             3138..4652
                     /locus_tag="VF547_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8262694.1"
                     /translation="MSGERNIRRVCIVGGGSAGWMAAAALSNTLRGGCRVDLVESDEI
                     GIVGVGEATIPPIRMFNQSLGIDENDFLRATQGSFKLGIEFVDWARIGHRYFHPFGPF
                     AADFDAVPLHHYWLRARARGDRTPLDDYSMAWAAASRERFDRPIPDRRMIQSTYDYAY
                     HFDAGLYGRYLRAFSERKGVVRHEGRVVDVRLRGEDGFVERLVLGDGREIEADLFIDC
                     SGFRGLLIGQALGVGYDDWTRWLPCDRAVAVPCAHGGGFTPYTRSTALAAGWQWRIPL
                     QHRIGNGYVYSSRHVSDDEAAATLLANLDGEALAEPRPLRFTAGRRTLFWEKNCIALG
                     LASGFMEPLESTSLHLVQSAVTRLLALWPDRDFDPLGAAEFNRITIEEYERIRDFLIL
                     HYRLTERDDSALWRQCAAMTVPEPLQYKIDHFRRYGRLVSTGPELFQNPSWLAVHIGQ
                     LNWPERYDPLVDERAEVDAAAILAGIRRVAAEAAGAMPTHRAYIDRHCKAPPLR"
     gene            4777..5544
                     /locus_tag="VF547_07460"
     CDS             4777..5544
                     /locus_tag="VF547_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX8262695.1"
                     /translation="MADSKRDWARLITKAALVLGVGSVLAALVAALGTATGMWGFRAG
                     LGSLRYLFFAAAAGAVLGLIGLVMTRRRGKLMLSNLVALVVALGFVLYLGNLVRTARS
                     VPAIHDVATDLDDLPQFARLTVRPDNLENIPDEGRPELKALPPEARWKALHRAHYSDL
                     RPVRVATPPAETVRRAAALARERGWDVASVDEKAGTMEATETSLFFRFRDDVVVRARP
                     APGGGSIVDMRSISRVGGSDVGMNAKRVRAFLRDLQR"
     gene            complement(5587..5865)
                     /locus_tag="VF547_07465"
     CDS             complement(5587..5865)
                     /locus_tag="VF547_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262696.1"
                     /translation="MKGGYAAVALLAGSLLATHAAVAAGRAKRKYDPNQMICESRQEI
                     GSRLKRIRVCHTALEWEEVRAQEKVGLMRKQFNGASGSGATNVNDAPK"
     gene            6063..>6807
                     /locus_tag="VF547_07470"
     CDS             6063..>6807
                     /locus_tag="VF547_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8262697.1"
                     /translation="MADGTRFDTHVSAERFALLVNSVTDYAIYMLDADGYVATWNPGA
                     RRFKGYEAEEIIGRHFSTFFTEEDRAAGVPARALETAAREGRFEAEGWRVRKDGTRFW
                     VNAVLDPIRNEAGELLGFAKITRDDTEKREAALALQESERRFRTLVQGVKDYALYMLD
                     VDGRVSNWNTGAEAIKGYKADEIVGQHFSRFYTEEDRARGEPQRALATAIAEGKYEME
                     AWRVRRDGTYFWASVVIDPIYDEEGRLIGF"
BASE COUNT         1003 a         2396 c         2287 g         1121 t
ORIGIN      
        1 accagctccc cgccgatcag ctcgccgagt tcctcggcct cgcgaagaag catgtcgacc
       61 atgtcgtcgg ccggcttccc gcccatgctg atttcatcgc gcgccattgc gccgtccccg
      121 agccgcgcgc ggccagtgca ggagctgccc agtgaacgac attgccaagg ccgcgaccaa
      181 gccgaagctg ggcttcgcgc agttgtggaa catcagcttc ggcttcttcg gaatccagat
      241 cggcttcgcg cttcagaacg cgaacatgag ccggatcttc cagtcgctcg gcaccagcct
      301 cgacgacctg ccggccctgt gggtggcggc gccgctcacc ggcctgctcg tccagccggt
      361 catcggccat atgagcgacc gcacctggct cggccgcctc ggccggcggc gtccctattt
      421 cctggggggg gcgatcctcg cggccctttc gctgttcgtc atgccgctcg cgccggcgct
      481 gctgttcgcc gcggtgatgc tgtgggtgct cgacgcgtcg ctcaacgtct cgatggagcc
      541 gttccgggcc ttcgtcggcg acatgctgag aaaggatcag cacagcgccg gctatgcggt
      601 ccagaccgcc ttcattgggg ccggcgcggt ggtcggctcg atcgtgccct gggcgctcga
      661 gcagatcggc gtcgccaacg tcgcgccgga cggaggcatt cccgacacgg tgcgctacgc
      721 cttctggttc ggcggcgccg ccctgttcct ggccgtgctg tggacggttc tgaccacccg
      781 cgaatacagc cccgagcaga tggccgagtt cgagggcgcg aggaccgagg aggagggcca
      841 ggtcgtccgg atgctcgccg cgcgcagctt cgcctcgagc ctggcctgga tcgcggccgg
      901 cgcgctgatc gtcttcgcgg tgcgccagct cggcctcgag aaggaggtgt acctgctcgg
      961 cggcctgctg atcgcctacg gcctggccag catcgtcgcg atccgcggcg cccggggcgg
     1021 cgcggcgccg ggcatgctcg gccacatcgt cggcgacttc tccggcatgc ccgagctgat
     1081 gaagcggctg gcgctggcgc aattcttcag ctggtccgcc ctgttcatca tgtggatcaa
     1141 caccaccccg gtggtcgccc aatatttcta cggctccgcc gatgcggcga gcccggccta
     1201 tcagcaaggg gcgaactggg tcggcgtcct gttcacggcc tacaacggcg tcgccgcgat
     1261 cgcggccctc accctgctgc ccttcctcgc ccggcggatc ggccgggtgc gcacccactt
     1321 cgccgccctg ctgtgcggcg ccgccggcta tgcgagcttc ttcttcctcg acgatccccg
     1381 gctgctgatc gccagcgagg tcggagtggg catcgcctgg gcgtcgatcc tcgccatgcc
     1441 ctatgcgatc ctcgcctcgg ctctgccgca gaggaagctg ggcatctaca tgggcctgtt
     1501 caacgtcttc gtcgtcgtcc cgcaattgct cgtggcgaca gtgatgggat cgatcatgaa
     1561 gcgcttcttc cccggcgagc cgatctggac gatggccttc gccgccggag tgatgatcct
     1621 cgcggcgctc gccatgctcc gggtcgagga gccgaaatga tcccccccgg aacggggagg
     1681 gggaccatgc gaagcatggt ggagggggcc agcctccgtg ccggctccat cgcctggccc
     1741 cctccaccgc acttcgtgcg gtccccctcc ccgttccggg gaggaatatg ggcgctcctc
     1801 ctcctgctat tcatcttcgc cacccccgcc gtctcggccg accgctacga cgccgtcgac
     1861 ctgtcgggcg tgaagcatcc cgactgggcg aaggacgccg tcctctacca gctcaacacc
     1921 cgccagttca cgccggaagg aacgttcagg gccgccgaaa ggcagcttcc ccgcctcaag
     1981 gcgctcgggg ccgacatcgt ctggctgatg ccggtccacc cgatcggcga gaagaaccgc
     2041 aagggaacgc tcggcagccc ctacagcgtg cgcgactatt acgcggtcaa tcccgagttc
     2101 ggcacgatgg acgacctcaa gtcctttgtc cgcgaggcgc acaggctgga catgcgggtg
     2161 atcctcgact gggtcgccaa ccacagcgcc tgggacaatc ccctcgtcgc ccagcatccc
     2221 gactggtacg accgcgactg gaagggcgac ttccgcccca cgccctggtg ggactggtcg
     2281 gacatcatcg acttcgacta tcgcaagacc gggatccgcc gctacatggc cggcgcgatg
     2341 cggttctggg tgagcgaggc cgatgtcgac ggcttccggg ccgacgtcgc cgcctacatg
     2401 ccgctcgact tctgggagcg ggtccgcgcc gatctcgacg aggtgaagcc ggtcttcatg
     2461 ctcgccgagg cgcagatgcg cgacctccac tacaaggcgt tcgacgccag ctacgcctgg
     2521 ggttggtacg gcgccgtctc cgacatcgcc aagggcaagg ccgacgtcgg cgcgctgttc
     2581 ggctattaca gcgagaatga gagcgcctgg ccggccggcg cgatgcgaat gacatatgtc
     2641 gagaatcacg atcagaacgc ctggcacggc acccagttcg agcagttcgg tccggccctg
     2701 acgaatgcga tcgtgctgtc ggtggcaggc gaggggatcc cgctcatcta caacggccag
     2761 gaagcgggca acccgaagcg cctggccttc ttcgagcgcg acccgatcgc ctggcgcgag
     2821 cacccgatcg gcgacctcta caagcgcctg ttcgccctca agcaggccaa tcgcgcactg
     2881 tggaacgcgc cctggggcgg gcggatggtg ggagtggtca acagcgcccc cgcgaaggtc
     2941 ttctcgttcg tccggcgcac gcggtcggac cgggtcttcg ccgtgttcaa cttctcgccc
     3001 gagccgcagc gcgtcacctt cggggagggg ccgcaccgcg gccgcttccg cgatttcgac
     3061 accggccggc agatcaccat cgacgcctcg accgagctgg cgctggctcc ctggagctac
     3121 cggctgatgt ccatagaatg agcggcgagc gcaacatccg ccgggtctgc atcgtcggcg
     3181 gcggctcggc gggatggatg gcggccgcgg ccctgtccaa cacgctgcgc ggcggctgcc
     3241 gggtcgacct cgtcgaatcg gacgagatcg gcatcgtcgg ggtcggcgag gcgaccatcc
     3301 cgccgatccg gatgttcaac cagtcgctcg ggatcgacga gaacgatttc ctgcgcgcca
     3361 cccagggcag cttcaagctc ggcatcgagt tcgtcgactg ggcccggatc ggccaccgct
     3421 acttccaccc gttcgggccc ttcgccgcgg atttcgacgc ggttccgctc caccattact
     3481 ggctccgcgc ccgcgcccgc ggcgaccgca cgcccctcga cgactattcg atggcctggg
     3541 ccgccgcctc ccgcgagcgg ttcgaccggc cgatccccga ccggcggatg atccagtcga
     3601 cctacgatta cgcctatcat ttcgacgcgg gcctctacgg ccggtacctg cgcgccttct
     3661 ccgagaggaa gggggtggtc cggcacgagg gcagggtggt cgacgtccgg ctgcgcggcg
     3721 aggacgggtt cgtcgagcgg ctggtcctcg gcgacggccg cgagatcgag gccgacctgt
     3781 tcatcgactg ctcgggattc cgcgggctgc tgatcggcca ggcgctggga gtcggctacg
     3841 acgactggac ccgctggctg ccctgcgacc gggcggtggc cgtgccctgc gcccatggcg
     3901 gcggcttcac gccctatacg cgctcgaccg cgctggcggc cggctggcag tggcggatcc
     3961 cgctccagca ccgcatcggc aacggctacg tctattcgag ccgccacgtc tccgacgacg
     4021 aggccgccgc gaccctgctc gccaatctcg acggcgaagc cctcgcggag ccgcggcccc
     4081 tgcgcttcac ggccggccgg aggaccctct tctgggagaa gaactgcatc gccctgggcc
     4141 tcgcctcggg cttcatggag ccgctcgaat cgaccagcct ccatctcgtc cagtcggcgg
     4201 tgacgcgcct gctcgcgctt tggccggacc gcgacttcga ccccctcggc gccgccgagt
     4261 tcaaccgcat cacgatcgag gaatatgagc ggatccgcga cttcctgatc ctccactacc
     4321 gtctcaccga gcgcgacgat tcggccttgt ggcgccaatg cgcggcgatg accgtgcccg
     4381 agccgctcca gtacaagatc gaccatttcc gccgctacgg tcggctcgtg tcgacggggc
     4441 ccgagctgtt ccagaacccg agctggctcg ccgtccacat cggccagctc aactggcccg
     4501 agcgctacga cccgctcgtc gacgagcggg ccgaggtcga tgccgcggcg atcctcgccg
     4561 gaatccgccg ggtggcggcc gaagcggcgg gggccatgcc gacccaccgc gcctatatcg
     4621 accgccactg caaggctccg cccttgcggt gatccttcga gacgcgttcg acgtcgctga
     4681 ccgtctactc aggatgagcg ggaccgtgta gcttgaccgc gcatctgagc aggaccgagc
     4741 ctggcgaagg ccgtatcgag ggacgaggag ggcgacatgg cggattcgaa gcgggactgg
     4801 gcgcggctga tcaccaaggc cgcgctggtg ctgggcgtcg ggtcggtgct cgcggcgctg
     4861 gtcgcggccc tggggacggc aacggggatg tggggcttcc gcgccgggct cggctcgctg
     4921 cgctacctgt tcttcgcggc tgcggcggga gccgtgctcg gcctcatagg cctcgtcatg
     4981 acgcggcggc gcggcaagct gatgctctcc aacctcgtcg ctttggtcgt cgcgctcggc
     5041 ttcgttctct atctcggcaa cctcgtccgg accgcgagga gcgtcccggc gatccacgac
     5101 gtcgccaccg atctcgacga tttgccgcaa ttcgcccggc tgaccgtcag gccggacaat
     5161 ctcgagaaca ttcccgacga gggcaggccg gagctcaagg cgctgccgcc cgaggcccgg
     5221 tggaaggccc tccaccgcgc gcactattcc gatctgcgcc cggtccgggt cgccacgccg
     5281 ccggccgaga ccgttcgacg tgccgctgcc ctggcgcggg aacgcggctg ggatgtcgcg
     5341 tcggtggacg agaaagcggg aacgatggag gcgacggaga ccagcctgtt cttccgcttc
     5401 agggacgacg tcgtcgtccg cgcccggccc gcgccgggcg gcggcagcat cgtcgacatg
     5461 cgctcgatca gccgagtggg cggcagcgac gtcggaatga acgccaaaag ggtgcgggcc
     5521 ttcctgcggg acctgcagcg ctgagccggg agccggaccc tagggggcct cgcggaattg
     5581 ccggggctat ttgggagcgt cgttgacgtt ggtcgcgccg gagccggacg cgccgttgaa
     5641 ctgcttgcgc atcagcccga ccttctcctg cgccctcact tcttcccatt ccagcgcggt
     5701 gtggcagacc cggatccgct tcaggcgtga gccgatctcc tggcggctct cgcagatcat
     5761 ctggttcggg tcgtatttgc gcttggccct cccggccgcg acggcggcgt gggtcgcgag
     5821 aagcgatccc gcgagcaaag caaccgcggc atatccaccc ttcatgtcgg cctccctgcc
     5881 gggaatggta tagggcgcag tgggccttgg caaacgggcc gcggcggtgg gaagcgggag
     5941 ggacctccct cgcgacgagc cgtgccgcgc ccagcgaagc ttggcggttg ccccagcgaa
     6001 atcagcagcg taggagcggc ctttgcccgc tttgtgcgca ggcctcctgg gcaggaccct
     6061 aaatggcgga cggaacgcgt ttcgacactc atgtctcggc cgagcgcttc gcgctgctgg
     6121 tcaactccgt cacagactac gcgatctaca tgctcgacgc cgacggctat gtcgccacct
     6181 ggaatccggg cgctcggcgc ttcaagggct atgaggccga ggagatcatc ggccgccatt
     6241 tctccacctt cttcaccgag gaggaccgcg ccgccggcgt tcccgcccgc gccctcgaga
     6301 cggccgccag ggagggccgt ttcgaggcgg agggctggcg ggtccgcaag gacggcacgc
     6361 gcttctgggt caacgccgtg ctcgacccga tccgcaacga ggccggcgag ctgctcggct
     6421 tcgccaagat cacccgcgac gataccgaga agcgcgaggc ggcgctcgcc ctgcaggaga
     6481 gcgagaggcg gttcaggacg ctcgtccagg gggtcaagga ctacgctctc tacatgctcg
     6541 acgtcgacgg ccgggtctcg aactggaaca ccggcgcgga agcgatcaag ggctacaagg
     6601 cggacgagat cgtcggccag catttctcgc gtttctatac cgaggaggac cgcgcgcgcg
     6661 gcgagcccca gcgggcgctg gcgaccgcca tcgccgaggg caagtacgag atggaggcct
     6721 ggcgggtgcg ccgcgacggc acctatttct gggccagcgt cgtcatcgac ccgatctacg
     6781 acgaggaggg tcggctgatc ggcttcg
//
LOCUS       DASVQZ010000274         5044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_633160, whole genome shotgun sequence.
ACCESSION   DASVQZ010000274 DASVQZ010000000
VERSION     DASVQZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633160"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..262)
                     /locus_tag="VF547_07475"
     tRNA            complement(187..262)
                     /locus_tag="VF547_07475"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Ala,seq:cgc)
     gene            526..1608
                     /locus_tag="VF547_07480"
     CDS             526..1608
                     /locus_tag="VF547_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX8262698.1"
                     /translation="MGHGSDDRSDNRWRDRDERDRSGWRGEGRDGYGRGGDDRGFFDR
                     AGDEVRSWFGDDDAQRRRERDERRWEQEQRMTGRRDNDPRSGGSGWNSGGWGNQRGES
                     WNRDRPGEPGGWDRDRNSEREGWFSSSVGGERGERWGGGQGRGENHGNDRIGRGGGGF
                     GGSPEQGRFERDDPGRVGSSGAMGQRFSSFGGGEHITRGGMGGDIHDPHYSEWRRRQI
                     EDLDRDYDEYRREHQSKFEQEFGGWRQKRASQRQMLGKVTEHMEVVGSDGTHVGKVDK
                     VRGDRIILAKSDPNAGGMHHSVPCGWVETVEDKVTLNRTASSAMDSWRNEDNGRALFE
                     REDQGSEGPHALDRSFSGTYGGKNDD"
     gene            1805..2803
                     /locus_tag="VF547_07485"
     CDS             1805..2803
                     /locus_tag="VF547_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8262699.1"
                     /translation="MSTDTNRAWHLKSRPQGMPTLDNFELKEAPIGELRDGWIRVENK
                     WLSVDPYMRGRMNDVKSYVPPFQVGEPMQGGAVGKVVESRSPDFAEGDTVFHMLGWRE
                     SAAGPAEQFNKVPPLPVEDHQWLGNLGLTGGTAYFGLLRAAQAKEGDIVFVSAAAGGV
                     GSAVVQIAKARGMTVIGSAGGAEKCAWVKELGADEVIDYKAEPIVPALLRAAPKGIDV
                     YFDNVGGEHLDAAFAAARQNARFAICGMIDSYNGGEPQAFRYIMRVIAARIMLKGFIY
                     TDYLAEMPDFYRDMGGLIAAGKLTSRETVRDGIEAMPDAFLDLFRGGNIGKMLVRL"
     gene            complement(2969..4351)
                     /locus_tag="VF547_07490"
     CDS             complement(2969..4351)
                     /locus_tag="VF547_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823239.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8262700.1"
                     /translation="MHSVTLASEDDLDGWRDAARALAGAGIPPAEVSWTVGGGPGDLF
                     AAPAPLPSGGTPFSVPRAFLTLAESAICHSDPERFALLYALLLRLRERPGLLADSADP
                     LVQRLERMAKSVRRDVHKMRAFLRFREVETPEGPRFVAWFEPEHHIVRHNGGFFVRRF
                     ANMAWSILTPELSLHWDGETLSEGPGAARSDAPEGDPIESVWKTYYASIFNPARLKKG
                     AMLKEMPKKYWKNMPETALVGELMAGAQAREAKMVEAGRGQVGGNLRSAWETLRDEAM
                     GCTRCHLYKCATQTVFGEGPLDARILFVGEQPGDQEDLAGRPFVGPAGQLFDRALGDA
                     GVDRKAAYVTNAVKHFKFEQRGKRRIHSKPDAGEIEACRWWIEQERLLLRPPLTVALG
                     ATAARSIFGRSVTITAMRGRAHALPEGGEAWVTVHPSFLLRVQDNKEAEYARFVEDLK
                     TIGERAKALA"
     gene            complement(4493..>5044)
                     /locus_tag="VF547_07495"
     CDS             complement(4493..>5044)
                     /locus_tag="VF547_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HEX8262701.1"
                     /translation="ADSASDSDIVGRASTLYDRFRLRRVYYSAFSPIPDASAVLPLRR
                     PPLMREHRLYQSDWLMRFYGFSPAEVASAADPAGMLPLDMDPKLAWALKFRESFPVDV
                     NRAPREALLRVPGLGVKAVDKLLASRRWRKLRLEDVARLTVSIAKVRPFICAEGWSPT
                     VLTDRADLAAWVKPAEKQMELFV"
BASE COUNT          852 a         1607 c         1830 g          755 t
ORIGIN      
        1 caacgcctac ttgcccgagt tctccggctg tcatgcccaa cgcgacgata tgcgatgcca
       61 tttggctccc ccttgcgggg aaggttgcac agcgaatcga ccaggggcaa cagcctcttc
      121 cccggccgct tttccgcacc ctttccgtac gacgcgctgg tctccggacc taagtcattg
      181 aaaaggtggt ggagctgagg ggaatcgaac ccctgacctc tgcagtgcga ttgcagcgct
      241 ctcccatctg agctacagcc ccgcgccttt tcggcggcca ggtttccccg cttgcgagcg
      301 gggctgggaa ggcgaccaga tagcggggcc gccgcacttt cgcaatgcgc caattgagcc
      361 gctcgccggg accgattccg ttcgccggga tcgcctctcg ggccgtctct tgacatggaa
      421 cagcttcgcg gctgcccccg ttgtcccctc aacccgcggc gcgcggaagg tccgcgccgc
      481 tatcgacgcg ggtcccgagc acaacccgag cacaaggagt tagacatggg acatggcagc
      541 gacgaccgct ctgacaatcg atggcgcgac cgcgacgagc gcgaccgctc aggctggcgc
      601 ggcgagggac gcgacggata tggacgcggc ggcgacgacc gcggcttctt cgaccgggcc
      661 ggcgacgagg tccgttcctg gttcggggac gacgacgccc agcgccggcg cgagcgcgac
      721 gagcgccgct gggagcagga acagcggatg accggccgcc gcgacaacga tccgcggtcc
      781 ggcgggtcgg gctggaatag cggcggctgg ggcaaccagc gcggcgagag ctggaatcgc
      841 gaccgtccgg gcgagccggg cggctgggac cgcgaccgca attccgagcg cgagggctgg
      901 ttctccagct cggtcggcgg agagcgcggc gagcgctggg gcggcggcca gggccgcggc
      961 gagaatcatg gcaacgaccg gatcggacgc ggaggcggcg gcttcggcgg atcccccgag
     1021 cagggccggt tcgagcgcga cgacccgggc cgggtcggtt cctcgggcgc catgggccag
     1081 aggttctcga gcttcggcgg cggcgagcat atcacccgag gcggaatggg cggcgacatc
     1141 cacgacccgc attattcgga atggcggcgg cggcagatcg aggacctcga ccgcgactat
     1201 gacgaatatc gccgggagca tcagtccaag ttcgagcagg agttcggcgg ctggcggcag
     1261 aagcgtgcaa gccagcggca gatgctgggc aaggtgaccg agcatatgga ggtggtcggc
     1321 tccgacggga cccatgtcgg caaggtcgac aaggtgcgcg gcgaccggat catcctcgcc
     1381 aagtccgatc ccaatgccgg cgggatgcat cacagcgtgc cctgcggctg ggtcgagacg
     1441 gtcgaggaca aggtgaccct caaccgcacc gcgtcgtcgg cgatggactc ctggcgaaac
     1501 gaggataacg gccgggcgct gttcgagcgc gaggaccagg gaagcgaggg accgcatgcc
     1561 ctcgatcgaa gcttctccgg aacctatggc gggaagaacg acgactgacg cccaggcctg
     1621 ggcgcaagga ggcccggccc tccccgtggg ggccgggcct ttttcgtcat tgcgaggagc
     1681 gaagcgactc ggcaatccag cggacggagg cgcggcactg gattgcttcg ccgccgcgaa
     1741 gcggcaattt accctgagcg ccgccgcagg cggcgtcgaa gggctcgcaa tgacggagag
     1801 agcgatgagc accgacacca atcgcgcctg gcacctgaag tcgcgcccgc agggcatgcc
     1861 gaccctcgac aatttcgagc tgaaggaggc tccgatcggc gagcttcggg acggctggat
     1921 ccgggtcgag aacaagtggc tttcggtcga cccctacatg cgcgggcgga tgaacgacgt
     1981 gaagagctac gttccgccct tccaggtcgg tgagccgatg cagggcgggg cggtcggcaa
     2041 ggtggtcgag agccgctctc cggatttcgc cgagggcgac accgtcttcc acatgctggg
     2101 ctggcgcgag tcggcggccg ggccggccga gcagttcaac aaggtgccgc cgctccccgt
     2161 cgaggaccat cagtggctcg gcaatctcgg cctcaccggc gggaccgcct atttcgggct
     2221 gctccgagcg gcccaggcga aggagggcga catcgtcttc gtctcggcgg ccgcgggcgg
     2281 ggtcggctcg gccgtggtcc agatcgccaa ggctcggggc atgaccgtca tcggctccgc
     2341 cggcggggcc gagaaatgcg cctgggtgaa ggagctcggc gccgacgagg tgatcgacta
     2401 caaggccgag ccgatcgtcc ccgccttgct cagggcggcg ccgaagggga tcgacgtcta
     2461 tttcgacaat gtcggaggcg agcatctcga cgcggccttc gccgccgccc gccagaatgc
     2521 ccgcttcgca atctgcggga tgatcgattc ctacaatggg ggggagccgc aggccttccg
     2581 ctacatcatg cgggtgatcg ccgcgcggat catgctgaag ggcttcatct acaccgacta
     2641 tctggcggag atgcccgact tctaccgcga catgggcggc ctgatcgcgg cgggaaagct
     2701 gaccagccgg gagacggtga gggacggcat cgaggcgatg cccgacgcct tcctcgacct
     2761 gttccggggg ggcaatatcg gcaagatgct ggttcgattg taatttgcct ccccatgcgg
     2821 agcatgggga gggggaccag gcgcagcctg gtggaggggt agatgcgagg cttccgccgt
     2881 ctcgcgctgg atcctctgac tccgagccag ctacccctcc accacccttc gggtggtccc
     2941 cctccccatc tggcgatggg gaggcaaatc aagcgagcgc cttcgcccgc tctccgatcg
     3001 tcttcaggtc ctcgacgaac ctcgcatatt cggcttcctt attgtcctgg acgcgaagca
     3061 ggaagctggg gtggaccgtc acccaggcct cgccgccttc ggggagggcg tgggctcggc
     3121 cgcgcatcgc ggtgatggtg accgacctgc cgaagatgga gcgggcggcg gtggcgccga
     3181 gggcgactgt gaggggcggg cggagcagaa ggcgctcctg ctcgatccac cagcggcagg
     3241 cctcgatctc ccccgcatcg ggcttggagt ggatccgccg cttgccgcgc tgctcgaact
     3301 tgaagtgctt gaccgcgttg gtgacgtagg cggccttgcg gtcgaccccg gcatcgccga
     3361 gcgcccggtc gaaaagctgc ccggcggggc cgacgaacgg tcggccggcg agatcctcct
     3421 ggtcgcccgg ctgctcgccg acgaagagga tccgggcgtc gagcggacct tcgccgaaga
     3481 cggtctgggt ggcgcatttg tagaggtggc agcgagtgca gcccatcgcc tcgtcgcgaa
     3541 gcgtttccca ggcggagcgc aaattgccgc cgacctgtcc tcgacccgct tcgaccatct
     3601 tcgcctctcg cgcctgggcg cccgccatca actcccccac caacgcggtc tcgggcatgt
     3661 ttttccagta tttcttcggc atctccttca gcatcgcgcc tttcttgagg cgggccggat
     3721 tgaagatgga ggcgtaatag gtcttccaga cggactcgat cggatcgccc tcgggcgcgt
     3781 cggagcgggc ggcgcccggc ccctcgctca gagtctcgcc gtcccagtgg agcgacagct
     3841 cgggggtgag gatcgaccag gccatgttgg cgaagcggcg gacgaagaag ccgccattgt
     3901 ggcggacgat gtgatgctcc ggctcgaacc aggcgacgaa gcgcgggccc tcgggagtct
     3961 cgacttccct gaagcggagg aaggcgcgca tcttgtggac gtcgcggcga accgacttgg
     4021 ccatgcgctc gagcctctgg acgagcggat cggccgagtc ggcgagcagg cccggccgct
     4081 cgcggagccg gagcaggagc gcgtagagca aggcgaaacg ctcgggatcc gaatggcaga
     4141 tggcggattc ggccaaggtg aggaaggcgc ggggcacgga gaagggcgtg ccgccggaag
     4201 ggagcggggc gggggcggcg aagagatcgc cggggccgcc gccgacggtc cagctcacct
     4261 ccgccggcgg gatgccggcg ccggcgagcg cccgggcggc atcgcgccag ccgtcgagat
     4321 cgtcttcact ggccaaggtg acgctgtgca tggacattgt tcgggctgag cctgtccttc
     4381 gatacgccgg ttcgacaggc tcaccggcta ctcaggatga gcggaagaga atgatcccaa
     4441 ccgctcatcc tgagtaggga ctgagcctgg cgaaggcccg tatcgaagga cctcaaacga
     4501 acaattccat ttgtttctcc gcgggcttta cccaggccgc caggtcggcg cggtcggtca
     4561 ggacggtggg gctccatcct tcggcgcaga tgaaggggcg gaccttggcg atcgagacgg
     4621 tgagccgggc gacgtcctcg aggcggagct tgcgccagcg gcggctggcg aggagcttgt
     4681 ccaccgcctt cactccaagc cccggaaccc ggagaagggc ttcgcgaggc gcccggttga
     4741 cgtcgaccgg aaagctctcc cggaacttga gcgcccaggc gagcttggga tccatgtcga
     4801 gcgggagcat cccggccggg tcggcggccg aggcgacctc ggcgggcgag aagccgtaga
     4861 agcgcatcag ccagtccgac tggtagagcc ggtgctcgcg catcagcggc ggacggcgaa
     4921 gcggaagaac ggcggacgcg tcggggattg gcgagaaggc cgaataatag acccggcgga
     4981 ggcggaagcg gtcgtagagg gtgcttgccc gcccgacgat gtccgagtcc gaggcggaat
     5041 cggc
//
LOCUS       DASVQZ010000275         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_825712, whole genome shotgun sequence.
ACCESSION   DASVQZ010000275 DASVQZ010000000
VERSION     DASVQZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825712"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VF547_07500"
     CDS             complement(<1..894)
                     /locus_tag="VF547_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262702.1"
                     /translation="MVAIFSGSGLGFQRSSASVVGTTGALGDAAFGRGGDNVMVNAAT
                     GNLIVSRKDEFLVGQGPDAAYAQTYNSHGDPAYAWSQSFYHVLTDIPATPNVAGSTIV
                     RGDGDGRNTVYTYDSARGAYLNTEGGGAYDELRYANGAWTWTDGDSRTRETYVALSGV
                     PGYQFLASVIDADGNTQSYSWYTNGTLQRITNANGDYVQFTQGSLGLPTQITTYTSGG
                     AALLTRTRYVWDSSRRLTSVTVDLSPGDHSVADGKSYTTTYTYDGTSSRIASISQTDG
                     SKLDIAYVLVGADYRVATLTQT"
     gene            complement(1085..1954)
                     /locus_tag="VF547_07505"
     CDS             complement(1085..1954)
                     /locus_tag="VF547_07505"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8262703.1"
                     /translation="MAANEPRSVWSVAAELGEGPVWVDSALWFTDIKGPRIHRYDPAS
                     GGRDSWEAPEQVGFVLPAQGGGFVAGLMSGLHHFDPATGGFAPIAQIEPERPNNRLND
                     GVVDPSGRLWFGTMDNGETEKTGAFYRFDRGAVVPTGLSGIAITNGPALSPDGRILYF
                     VDTLAGTIDAADVAQDGSLGPPRRFARIDPADGFPDGPTVDSEGCVWISLYAGWEAWR
                     FSPDGERIGRVRFPVANITKLAFGGEDLRTAYATTARQLLSPEALADQPQAGDLFEFR
                     VDVPGLPCPSIAV"
     gene            complement(2087..>3657)
                     /locus_tag="VF547_07510"
     CDS             complement(2087..>3657)
                     /locus_tag="VF547_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8262704.1"
                     /translation="DGVDEMQTSGAQRISIVGERAPIFWGACAAIAIGVLLHLPMLAM
                     AHDMGNRLAGMPMDPKMWVGMALIVLGVPAAVFGALPRQRASHGAEAGTNYEAPDSTP
                     LNRWHAGVLLVLIVGLVIDVMKPATLGFVLPGMSHEYGISTTTVAWFPFTALVGTTIG
                     SFVWGWLADIYGRRVSILLSTILFVSTSICGAMPLFQLNLLMCFLMGCSAGGMLPVVY
                     TLLAEIMPPRHRSWVLVLVGGTGLVGGYLAASAAAHAFEPVFGWRVLWLQGFPTGLLL
                     LALAFLIPESPRFLAEQGRVAELAEMRRKFGIVPRPREPAPADAEVAARRHGRLTFAL
                     VITALAWSFVNFGLLLWLPSDLQARGYSSALASGILASSALIALPTIMGAAWSYSRLS
                     SKWTLVATIVLTLGGLIGALLPASILSWPPLLIAVIALLIVGSNGMIAVLLPYSAENY
                     PLGIRGRATGLVAGSSKFGGVAVQGFALLGFIPTLAGAAFGLLAPMGLSAVLIAWAGR
                     ETRGRSLRELEASA"
BASE COUNT          594 a         1200 c         1326 g          537 t
ORIGIN      
        1 cgtctgggtg agagtcgcca cgcggtagtc ggcgccgacc aggacatagg cgatgtcgag
       61 cttggagccg tcggtctggc tgatcgaggc gatccgcgac gaggtgccgt cgtaggtgta
      121 ggtggtggtg tagctcttgc cgtcggccac cgaatggtcg ccggggctga ggtcgacggt
      181 gacgctggtc aagcggcgcg aggaatccca gacgtagcgg gtgcgcgtga gcaaggcggc
      241 gccgcccgac gtgtaggtcg tgatctgggt cggcaggccg aggctgccct gcgtgaactg
      301 gacatagtcg ccgttggcgt tggtgatccg ctgcagcgtg ccgttggtgt accagctgta
      361 ggattgggtg ttgccgtcgg cgtcgatgac gctggcgagg aactggtagc cgggcactcc
      421 ggagagggcg acgtaggtct cgcgggtgcg gctgtcgccg tcggtccagg tccaggcgcc
      481 gttggcgtag cggagctcgt cataggcgcc gccgccctcg gtgttcagat aggcgccgcg
      541 ggccgagtcg taggtgtaga cggtgttgcg gccgtcgccg tcgccgcgaa cgatcgtgct
      601 gccggcaacg ttgggagtcg cggggatgtc ggtcagcaca tggtagaagc tctggctcca
      661 ggcgtaggcc gggtcgccat ggctgttgta ggtctgggca taggcggcgt cggggccctg
      721 gccgacgagg aactcgtcct tgcggctgac gatcaggttg ccggtggccg cgttgaccat
      781 gacattgtcg ccgccgcgcc cgaatgcggc atctccgagc gctccagtcg ttccgacgac
      841 cgacgccgag ctgcgctgga atcccaggcc gctgccagag aaaatcgcta ccatttgccc
      901 ctccccacga cgaggggatt gggaacgccc ccgctatccc attgatcgga taattatcga
      961 attagtttag gcttttttta aatctatttt tggcgagccg gttttattcg ggattttcgg
     1021 tccggattcg gaggcgccga ccgagggaaa tcgcgaccgt ccgaaaggcc ggccgccgag
     1081 gccgtcagac cgctatcgac ggacaaggca gtcccggtac gtcgacccgg aactcgaaca
     1141 ggtcgccggc ctggggctgg tccgcgagcg cctcaggcga cagcagctgg cgggcggtgg
     1201 tggcgtaggc cgtccgcagg tcctcgccgc cgaaggcgag cttggtgatg ttggcgaccg
     1261 ggaagcgcac ccggccgatc cgctcgccgt cgggcgagaa acgccaggct tcccagccgg
     1321 cgtagaggct gatccagacg cagccctcgc tgtcgaccgt cggcccgtcc ggaaagccgt
     1381 cggccggatc gatgcgcgcg aaccgcctcg gcggtcccag gcttccgtcc tgcgcgacgt
     1441 cggcggcgtc gatggtcccg gcgagggtgt cgacgaaata gaggatccgg ccgtccgggg
     1501 acagggccgg gccgttggtg atggcgatgc cgcttaggcc ggtcggcacc accgccccgc
     1561 ggtcgaagcg gtagaaggca ccggtcttct cggtctcgcc attgtccatg gttccgaacc
     1621 acagccgtcc ggaaggatcg acgacgccgt cgttgaggcg gttgttcggc cgctccggct
     1681 cgatctgcgc gatcggggca aagcctccgg tagccgggtc gaaatgatgg aggccgctca
     1741 tcagcccggc gacgaagccg ccgccctgcg ccggcaggac gaagccgacc tgctcgggcg
     1801 cctcccagct gtcgcgcccg ccgctcgccg ggtcgtagcg atggatcctt ggccccttga
     1861 tgtcggtgaa ccacaaggcc gagtcgaccc agaccggccc ttcgccgagc tcggccgcga
     1921 cgctccagac gctcctcggt tcattcgccg ccattgcgga cgctcctgcc agatgctcgc
     1981 ccgaggccgg ccgccggagc gcgccggggc gagaatcgcc ataccccata ggtaacgcca
     2041 tggcgtccgg gaagggccgg cacgcgccgg cggcggcgtc ggatcgtcat gccgaggctt
     2101 cgagctcgcg caggctccgg ccgcgggttt cccggcccgc ccaggcgatc agcacggccg
     2161 agaggcccat cggcgcgagc aggccgaacg cggcgccggc cagggtcggg atgaagccga
     2221 gcaaggcgaa gccctgcacg gccacgccgc cgaacttgct cgatccggcg accaggccgg
     2281 tggctcggcc gcggatgccg agcgggtaat tctcggcgct gtaggggagg aggacggcga
     2341 tcatgccgtt cgagccgacg atcaggagcg cgatgacggc gatcagaagc ggcggccatg
     2401 acaggatcga cgccggcagc agcgcgccga tcaggcctcc gagcgtgagc acgatcgtcg
     2461 cgaccaaggt ccatttgctg ctcagccggc tgtagctcca ggccgcgccc atgatcgtcg
     2521 gcagcgcgat cagggcggag ctggcgagga tcccgctcgc cagcgccgag ctgtagccgc
     2581 gcgcctggag gtccgacggc agccacagca gcaagccgaa attgacgaag ctccaggcca
     2641 gggcggtgat caccagagcg aaggtcagcc gcccgtgccg gcgcgccgcg acctcggcgt
     2701 cggccggggc cggctcccgc ggccgcggaa cgatgccgaa cttgcggcgc atctcggcga
     2761 gctcggcgac ccggccctgc tcggcgagga agcgcggcga ttccgggatg aggaaggcga
     2821 gcgcgagcag cagcaggccc gtcgggaagc cctgcagcca cagcacgcgc cagccgaaga
     2881 cgggttcgaa ggcatgggcc gcggcgctgg cggcgagata gccgccgacg agcccggtgc
     2941 ctccgaccag caccagcacc caggagcggt ggcgcggcgg catgatctcg gcgagcaggg
     3001 tatagaccac cggcagcatt ccgccggccg agcagcccat caggaagcac atcagcaggt
     3061 tcagctggaa aagcggcatc gcgccgcaga tcgaggtcga gacgaacaat atggtggaga
     3121 gcaggatcga gacccggcgg ccgtagatgt cggccagcca tccccagacg aacgagccga
     3181 tcgtggttcc cacgagcgcg gtgaacggga accacgcgac cgtcgtcgtc gagatgccgt
     3241 attcatggct catgcccggc agcacgaagc ccagggtggc gggcttcatc acgtcgatga
     3301 cgaggcccac gatcagcacc agcaggacgc ccgcatgcca gcggttgagg ggagtcgaat
     3361 cgggcgcctc gtaattggtg ccggcctcgg cgccgtggga cgcacgctgc ctaggcagcg
     3421 ccccgaaaac cgccgccggc acgccgagca cgatcagggc catgcccacc cacatcttcg
     3481 ggtccatcgg catgccggcg aggcggttgc ccatgtcgtg cgccatggcg agcatcggca
     3541 ggtgcagcag caccccgatc gcgatcgcgg cgcacgcgcc ccagaagatc ggcgcgcgtt
     3601 cgccgacgat cgatatgcgc tgcgcgccgc tggtctgcat ctcgtccacc ccgtcgc
//
LOCUS       DASVQZ010000276        12036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_56216, whole genome shotgun sequence.
ACCESSION   DASVQZ010000276 DASVQZ010000000
VERSION     DASVQZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56216"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VF547_07515"
     CDS             <1..1546
                     /locus_tag="VF547_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262705.1"
                     /translation="DNRLLHRALGLLPILNDAERERIARFSEAAAQEGEGVAVATALR
                     LAHAPLKDQFAILLSTRQDRWPRDYKYLLNDPDEEELEERIRALDLSLPRDKIWPAAV
                     VVRQALRRFGIPNPIEHCWTQAFEHPADEVAALAIEIADAAGGVAAARALAATGWTAA
                     DAANDLRAFEGSSLLLKLPDDELRTQLHRLCPETLAYVYLHREPLREGAASLWQAWME
                     DQLLVARTSRTFGGGYYRYQDRDEAYALFRRDRPEEALRLIRTAWESKALRGNIQMDH
                     AEGPAWPLLKALAGDHPELVKTIWRETVEEHGVMWIGGIEQFPAQLPPGREFDDVRAE
                     MLRGANSDEKLFDDVRRLEESGHLDFLFSYIEAGLAGGRAVDQARAVAVAGFLDGSER
                     AVKLWKGPLRAAPGKGWLETVHRHARAWFDRVVQARHWQRGIAAATDEREALRGWWLL
                     ARVVDDRYPQYYDCGGCRVDPQSWRAQWLDFFIESAKSLRKNARTPLKDSWLHGKRHH
                     DVINGR"
     gene            1634..2260
                     /locus_tag="VF547_07520"
     CDS             1634..2260
                     /locus_tag="VF547_07520"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX8262706.1"
                     /translation="MAATAKTDHALERLVFFSDAVFAIAITLLVIEIHAPHLPEGSPD
                     RAYWLALARMWPSLLGYFVSFAVIGLFWMGHHRAFALAGRYHPKILPWNMGLLLTIAF
                     MPFVTAWLAQNLYARPPALLYCAAMLAAAGFNLKVVRTATGPGMVDPKVGAADVAYVR
                     ARSLSVVLGSATAVALSLVLPQMAQAGLVTIPLWRLALTRRAKAEPAA"
     gene            2257..2778
                     /locus_tag="VF547_07525"
     CDS             2257..2778
                     /locus_tag="VF547_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8262707.1"
                     /translation="MSRKVRFGGAVSLDLYFAGPGEAIDWLRWSEDSAKIVAASWKGV
                     DTMLIGRKTWEFARRSDGGPALSGVTTYLFSRTMTESPPGAVLVREDAAGFVRRLKAG
                     PGGDVVVMGGGELGSALIEAGLVDELGLSVHPVLLGGGIPVFRPMARRVEFELIEARP
                     MARDCVLLRYRAA"
     gene            2971..3648
                     /locus_tag="VF547_07530"
     CDS             2971..3648
                     /locus_tag="VF547_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262708.1"
                     /translation="MGRRRIIEPSSLRDGGGGRFSWDDAFDRRPTGIEEAAARVGPAG
                     SDPLVEAALAAVTERVPEIVHARQDKLSQENIDLLVDAYLRSAPTAPARHEIEMDNAR
                     ERARFVEEFPCYTSREVAELAGHEAANASATATRWKKAGRIIGLPWKGSDLYPAFQFS
                     EGRPRPGIGRAIAALPAGRSPWQIAFWLTSGNGWLSGATPLERLESGDEPAVLAAAGR
                     EGEAFEG"
     gene            3678..4277
                     /locus_tag="VF547_07535"
     CDS             3678..4277
                     /locus_tag="VF547_07535"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX8262709.1"
                     /translation="MNRQRLPAGVELHRIHAAERPANGFNPGKGGPTRFAPLVRPDNT
                     PIPTAYAAEDYECAAHETIFHEIPYHAARKSIAISKIGALSHSVVRTRRELVLAALFE
                     PDLNSWGLTRGKLIDTSADDYPDSARWALALHERWADIDGLVWTSRRCDPRRAYLLFG
                     DRVAEADLEPVAEVRIIASNDSLAALRRFGARAGIALVL"
     gene            complement(4719..4988)
                     /locus_tag="VF547_07540"
     CDS             complement(4719..4988)
                     /locus_tag="VF547_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262710.1"
                     /translation="MDKRIAVTPAELGRAIRVWLRVMPKHVWRALQKYELAALDKRQQ
                     PDEEPQVHAAVADHIVGQFERARWDVTRPEPPTDLGSPPPWNGSR"
     gene            5052..5648
                     /locus_tag="VF547_07545"
     CDS             5052..5648
                     /locus_tag="VF547_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX8262711.1"
                     /translation="MCNLYRQRNTESLAKLFDAVPLELPFEARPDIYPKYEAVVVRKE
                     GGRRRLDCMAWGIVRTMPGKSGKPVKKAVTNVRNLDSPFWRSTIANPAQRCLVPFTSF
                     AEPRPGKDPETGRPANWWFTIEGEETCAFAGIWRWSEDRARFAFLTCEPNPLVAPLHP
                     KAMPVILQPEDHDRWLDGEAADACALAQPFPSQLMRVA"
     gene            complement(5699..5935)
                     /locus_tag="VF547_07550"
     CDS             complement(5699..5935)
                     /locus_tag="VF547_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262712.1"
                     /translation="MPRYFFHLYDDVVSLDEEGRDMPDLESARAAGVREARQMMVGTV
                     AEGRLDLSHRIDIADESGAVLATVAFRDAVTVTG"
     gene            6426..7019
                     /locus_tag="VF547_07555"
     CDS             6426..7019
                     /locus_tag="VF547_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262713.1"
                     /translation="MNRRRHALRGRNGRYVAAAAKAEEPGLELKRGRGDRVGGRPQIR
                     RKRKDALTESEKETFLSTFAETCNIAHSARTVGRKERVFRDLKKRDPGFAADWMATLR
                     ESYDLLEAEMARRARFGTRKDVFHQGRRVARIRVFNDAMALRLLSLHRSSVERMRAAE
                     SAPRRDAKTLFDELAARVAEIKAKRAKDAEGGDHEAA"
     gene            7006..8322
                     /locus_tag="VF547_07560"
     CDS             7006..8322
                     /locus_tag="VF547_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX8262714.1"
                     /translation="MKPPEWMSLAEEIADLGPAERAFVLKTLNAAEDAGLRYLWRLWE
                     RRDQREPQLPWRTWLVMAGRGYGKTRIGAEWVRRMAASDPTACIALVGATREQARAWM
                     VENDNGILGVCPPDERPAWEPSLGRLKWPGGATAFVYSAAEPESLRGPQHHFAWCDEI
                     AKWPFGPEAWSNLMLGLRRGSLPRAVATTTPRPVALVRRLVAQGVAMTRGRTVQNASN
                     LPGFFIDEIRRDYGSGRLGRQELDGELIDDVEGSLWPRDLIERCRAVSPPPELRRVVV
                     AVDPPASAQGTCGIVVCALGGDGLGYVLADATVVGLRPEGWARKVEAAASAWNADRVI
                     GEGNQGGAMVESVLRGAGVALPVKLVYAHPGKTARAEPVAALFEGGRARFAGGFPELE
                     DQLAGMVAGGDYEGPGRSPDRADAMVWAMTELMLRRRRAEPRIRSL"
     gene            8428..8718
                     /locus_tag="VF547_07565"
     CDS             8428..8718
                     /locus_tag="VF547_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262715.1"
                     /translation="MDLVRVATGMAVLLASGAPAAAAPRKDQDPDRQICKSKPVIGSR
                     LKRVRECHSAAEWEDLKLAEQIGLGRQQFNGADGLGARESYLPPGAAAQQPN"
     gene            8769..9068
                     /locus_tag="VF547_07570"
     CDS             8769..9068
                     /locus_tag="VF547_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262716.1"
                     /translation="MKLIRIAAGAALIAAASAPGAAAPKGKAGYDPDRQICKSRAVVG
                     SRVKRIRECATAQQWEDMELQERMGLMRKQINGDPGCSGGHSDSCHPMGVKDSPI"
     gene            9097..9396
                     /locus_tag="VF547_07575"
     CDS             9097..9396
                     /locus_tag="VF547_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262717.1"
                     /translation="MRISAALAGLALLATAFAPAAAAPRKGKPAYDPDRQICKSKPVV
                     GSRLKRIRECHSAAEWEDLELQERVGLMRKQVNGDPGCNGGGQCGIQNGGRDTPW"
     gene            9417..9695
                     /locus_tag="VF547_07580"
     CDS             9417..9695
                     /locus_tag="VF547_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262718.1"
                     /translation="MRMVKLMAVLAVLAAAPAAAAPKKGEPGYDPDREICKSKPVVGS
                     RLKRVRECATAAQWEEMKLQERQGLLRRQTNGDPSNAPVAAGGRDTPF"
     gene            9727..10020
                     /locus_tag="VF547_07585"
     CDS             9727..10020
                     /locus_tag="VF547_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262719.1"
                     /translation="MKRIAMAAALVLPGAMPGAAPAATKGRAADDPGRQICRSKPVVG
                     SRLKKIRECHSAAEWEDLELQERMGLRRQQFNGAPGCPQPPCVAAPGGRDTPW"
     gene            10040..10345
                     /locus_tag="VF547_07590"
     CDS             10040..10345
                     /locus_tag="VF547_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262720.1"
                     /translation="MTSARIMAGLALLAAASMPGSPAAAAKKGAGAEDPDRQICKSRS
                     VIGSRLKRIRECHSAAEWEDLELQERMGLRRQQFNGDPGCAEAVGCHLRPGSKDSPL"
     gene            10476..11591
                     /locus_tag="VF547_07595"
     CDS             10476..11591
                     /locus_tag="VF547_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX8262721.1"
                     /translation="MKFWFGQKAAQPQARPPLHRAYAASAAAGDWPRGYEAQVRDAYL
                     GNPVAQRAVRLVAESVAWAPVYALDGAAGAAASAKAVGLVGPPLLDSAAAQLLLHGNA
                     FVQILLDSDGAPAELFALRPERVTVEADAGGWPAAYVYRAGEVKTRLPARDALGRPSL
                     IHVKAMHPLDDHYGLGCLGAAAGAVAIHNSATRWNKALLDNMARPSGALSLDGDEATL
                     APEQYQRLRDELELQFGGEGNAGRPMILEGGLKWQPMSLTPADMDFVALKAAAAREIA
                     LAFGVPPMLLGLPGDSTYANYREANRALWRLTVLPLAERLLGGIAAALAAWWPGLRLR
                     LDVDQVTALAEDRERLWAQVSSADFLTREEKREMLGF"
     gene            11631..12020
                     /locus_tag="VF547_07600"
     CDS             11631..12020
                     /locus_tag="VF547_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8262722.1"
                     /translation="METRAIVEALVEAINGGRAERVAAAMSLDAVFVDSLGRRIEGRR
                     DLTEGWRGYFRLFPDYRIEVDGLIVEGPEALLHGRARGTLHRDGHAVDGGGGEVPAAW
                     RAVSDGRRVALWQVYADNKPVYALLGM"
BASE COUNT         1828 a         3943 c         4586 g         1679 t
ORIGIN      
        1 ggacaatcgc ttgctccacc gtgccctggg tctcctgccg atcctgaacg acgccgagcg
       61 agagcgaatt gcgcggttct cggaagccgc cgcgcaggaa ggcgaagggg tcgctgtcgc
      121 aaccgcgctg cggctggccc atgcgccgct gaaagaccag ttcgccatcc tgctctcgac
      181 caggcaggac cgatggccgc gagattataa gtaccttctg aacgatccgg acgaggagga
      241 gctggaggag cgaatccgag cgctggattt gtcgttgccg cgcgacaaga tctggccggc
      301 cgcagtcgtg gtccggcagg ccttgaggcg cttcggcatc cccaatccga tcgagcattg
      361 ctggacacag gccttcgagc atccggccga cgaggtagcc gccctcgcca tcgagatcgc
      421 cgacgccgcc ggaggggtag cggcggcccg cgcgctcgcg gcgaccggct ggaccgcagc
      481 cgacgccgcc aacgacctgc gcgccttcga aggttcgtca ctcctgctca aactgccgga
      541 cgacgagctt cgaacccagc tgcaccgcct ttgcccggag acgctggcct acgtctatct
      601 tcaccgcgag ccgcttcgcg agggcgcagc ttcgctctgg caggcgtgga tggaggacca
      661 gctgctggtg gcgagaacga gccgaacttt cggcggcggc tactaccgct accaggaccg
      721 ggacgaggcc tatgcccttt ttcgccgcga ccggccggag gaggcgcttc gactgatcag
      781 gacggcctgg gagagcaagg ccctgcgcgg caacatccag atggaccatg cggaagggcc
      841 ggcctggccg ctgttgaaag ccctggcggg agaccatccg gagctcgtca aaaccatctg
      901 gcgggagacc gtggaggaac acggcgtgat gtggatcgga ggcatagaac aattccccgc
      961 gcaattgcct ccgggccggg aattcgacga cgtccgggcc gaaatgctgc gtggcgccaa
     1021 cagcgacgag aagcttttcg atgacgttcg ccgactggaa gagtcggggc acctcgactt
     1081 cctgttctcc tatatcgagg cgggcctggc gggggggcga gcggtcgacc aggcgcgcgc
     1141 agtcgccgtg gccggcttcc tggacggatc cgagcgggcc gtgaagctct ggaaggggcc
     1201 gctacgcgcc gcaccgggca agggatggct ggagaccgtg catcgacatg ccagggcgtg
     1261 gttcgatcgg gttgtccagg cgcggcactg gcagcgcggg atcgccgcgg ctaccgacga
     1321 gcgcgaggcg ctccggggct ggtggctgct cgctcgagtc gtcgacgatc gatacccgca
     1381 atattatgat tgcgggggat gcagggtcga cccgcaaagc tggcgggcgc aatggctgga
     1441 tttcttcatt gagagcgcaa agtcgcttcg gaagaatgcc cgaacgccgc tcaaggacag
     1501 ttggctgcac ggcaagaggc atcacgacgt gatcaacggg cgataatctc gctcgaggcg
     1561 aagggcggcc tccctctggc ccccttgccc gccccgtggc atgttcggcg ccgccacggg
     1621 gggagacgcg atcatggccg cgaccgcgaa gaccgatcat gcgctggagc ggctcgtctt
     1681 cttctccgac gcggtgttcg cgatcgcgat cacgctgctc gtcatcgaga tccacgcccc
     1741 ccatctgccc gagggctcgc ccgaccgggc ctattggctc gcgctcgccc ggatgtggcc
     1801 gagcctgctc ggctatttcg tcagcttcgc ggtcatcggc ctgttctgga tgggccatca
     1861 ccgcgccttc gccttggccg gccgctatca cccgaagatc ctgccctgga acatggggct
     1921 gctcctgacc atcgccttca tgccattcgt caccgcctgg ctcgcccaga acctctacgc
     1981 ccgcccgccc gcactgctct attgcgcagc gatgctcgcc gccgccggct tcaacctcaa
     2041 ggtggtccgg acggcgaccg gcccgggcat ggtcgacccg aaggtcggag ccgccgacgt
     2101 cgcctatgtc cgcgcccggt cgctgagcgt cgtgctcggc tcggcgacgg cggtcgcgct
     2161 cagcctggtg ctcccgcaaa tggcgcaggc cggcctggtc accatcccgc tctggcgcct
     2221 ggccctgacc cggcgggcga aggccgagcc ggcggcatga gccgcaaggt gaggttcggc
     2281 ggggcggtca gcctcgatct ctatttcgcc gggccgggcg aggcgatcga ctggcttcgc
     2341 tggagcgagg attcggcgaa gatcgtggcg gccagctgga agggcgtgga cacgatgctg
     2401 atcggccgca agacctggga gttcgcccgg cgcagcgacg gcggcccggc cctgtcgggc
     2461 gtgacgacct acctcttctc gaggacgatg acggagtcgc cgccgggggc cgtgctggtc
     2521 cgcgaggacg ccgccgggtt cgtccggcgg ctcaaggccg ggccgggcgg cgacgtcgtc
     2581 gtcatgggcg gcggcgagct cggctcggcg ctgatcgagg cggggctggt cgacgagctc
     2641 ggcctcagcg tccacccggt gctgctcggc ggcggcatcc cggtgttccg gccgatggcc
     2701 cggcgggtcg agttcgagct gatcgaggcg aggccgatgg cgcgggactg cgtgctgctg
     2761 aggtaccggg cggcctgagg gcgagcgggc ggcgaggcgc tgcttcccac ccgaagcacc
     2821 gaaggaccga aggggctgga ggggcggcca ggcgggggct cggcgatcgg gctcagggtc
     2881 caggccgacg tccggacgcg gtcggactcg acccatttgc gggcagcagt tatatgggtt
     2941 atgagaaagt gctatcaccg agatcattct atgggacgcc gccgcatcat cgaaccttcc
     3001 agtctgcgcg acgggggcgg cggccggttc tcgtgggacg atgctttcga tcgacgcccg
     3061 acgggcatcg aggaggccgc ggcgcgggtc gggccggccg ggtccgaccc gctggtcgaa
     3121 gccgcgcttg ccgccgtgac cgagcgtgtg cccgagatcg tccatgcccg ccaggacaag
     3181 ctcagccagg agaacatcga ccttctcgtc gacgcctatc tgaggagcgc gcccacggcc
     3241 ccggcccgcc atgagatcga gatggacaat gcgcgcgagc gggcccggtt cgtcgaggaa
     3301 ttcccctgct acacgagccg ggaggtggcc gagctggccg ggcacgaggc ggccaatgcc
     3361 agcgccacgg cgacccggtg gaagaaggct gggcggatca tcgggctgcc ctggaaggga
     3421 agcgacctct acccggcttt ccagttcagc gagggaaggc cgcggccggg gatcggtcgg
     3481 gccatcgcgg cgcttccggc cgggcggtcg ccctggcaga tcgctttctg gctgacctcc
     3541 ggcaacggtt ggctgagcgg cgcgactccg ctcgagcggc tcgaatccgg cgacgagccg
     3601 gccgtgctcg ccgccgccgg ccgcgaaggc gaggccttcg agggatgatt ccgccaccgc
     3661 cggatccctt tccggcggtg aatcgtcagc ggctgccggc cggcgtcgag ctgcaccgaa
     3721 tccacgccgc cgagcgtccg gccaacggct tcaacccagg caagggcggg ccgacccgct
     3781 tcgcgccgct ggtccgcccc gacaataccc cgatacccac ggcctatgcg gctgaggatt
     3841 acgaatgcgc ggcgcacgag acgatattcc acgaaatccc ctatcacgcg gcgcgcaagt
     3901 cgatcgcgat cagcaagatc ggcgccttgt cgcactcggt cgtccggacc cggcgcgagc
     3961 tggtgctcgc ggccctgttc gagccggacc tcaattcctg ggggctgacc cgggggaagc
     4021 tgatcgacac ctcggccgac gactatccgg acagcgcgcg ctgggcgctc gcgctgcacg
     4081 aaaggtgggc ggatatcgac ggcctggtct ggacctcgcg gcgctgcgac ccccggcggg
     4141 cctacctgct gttcggcgac cgggtggcgg aggccgacct cgagccggtc gccgaggtcc
     4201 ggatcatcgc ctccaacgat tcgctcgccg cgctgaggcg cttcggcgcg cgggccggga
     4261 tcgccctcgt cctttgaggc cgggcgccgg cgaagccggt ggcgcggaga tcggtgcctt
     4321 cgatttgccg cagcgcgctt cggtgcttcg gtgcttcggg ggcttcgggt gggaaaggga
     4381 gggccttgca cgtatggagc gtccgcagtt cgggcgaccg cggcctgcag gtctttcacc
     4441 cgaagcaccg aaggaccgaa ggggttggag cgggggtcgg gccggggctc tgcgaatgag
     4501 ttcgtcgagc gacgcagccg ctcctcatgc ggggctcgtc atgacgggcg cggcgcttgg
     4561 aaggcctcgg cctcttcggt gcttcggtgc ttcgggtgac cttttcgtct cggcgacgga
     4621 gcagccgcct cagcccggca aggcaagccg cgtgccagac tcgtttggtg cctggcaccg
     4681 aaatgcgggt tgtcgcgggg cgagcacggg tcgccgaatc agcggctgcc gttccagggc
     4741 gggggggagc cgaggtcggt ggggggctcg gggcgggtga cgtcccagcg ggcgcgctcg
     4801 aactggccga cgatatggtc ggcgacggcg gcgtggacct gcggctcctc gtcgggctgc
     4861 tgccgcttgt cgagcgcggc gagctcgtat ttctgaagcg cgcgccagac gtgcttgggc
     4921 atcacccgaa gccacacccg gatggcgcgc ccgagctcgg cgggagtcac ggcgattcgc
     4981 ttgtccatgg agaacaaata gcgaacaaca cgccggcttg gaagccgcca caggcctgct
     5041 atcgtcgctc catgtgcaat ctctaccgcc agcgcaacac cgagagcctc gcgaagctgt
     5101 tcgacgccgt cccgctcgag ctgccgttcg aggccaggcc cgacatctat ccgaaatacg
     5161 aggcggtggt ggtgcgcaag gaaggggggc ggcgccggct cgactgcatg gcctggggga
     5221 tcgtgaggac gatgccgggc aagagcggca agccggtcaa gaaggcggtg accaacgtcc
     5281 gcaacctcga cagcccgttc tggcgctcga ccatcgccaa cccggcgcag cgctgcctcg
     5341 tcccgttcac ctcgttcgcc gagcctcggc cgggcaagga cccggagacc gggcggccgg
     5401 cgaactggtg gttcacgatc gaaggcgagg agacctgcgc cttcgcggga atctggcggt
     5461 ggagcgagga ccgggcgcga ttcgccttcc tgacctgcga gcccaatccg ctggtcgcgc
     5521 cgctccaccc caaggcgatg ccggtgatcc tgcagcccga ggatcacgac cgctggctgg
     5581 acggcgaggc ggccgacgcc tgcgccctcg cccagccctt cccgagccag ctgatgcggg
     5641 tggcgtgatc cgggctcggc cgccgccggc cggcgccgcc cgagcgggcc ggcgcgcttc
     5701 agccggtgac ggtgaccgcg tcgcggaagg cgacggtggc gaggacggcg ccgctttcgt
     5761 cggcgatgtc gatgcggtgg gagaggtcga gcctcccctc cgccaccgtg ccgaccatca
     5821 tctggcgcgc ctcgcggacg ccggcggcgc gggccgattc gagatcgggc atatcgcgcc
     5881 cttcctcgtc cagcgagacg acgtcgtcgt agagatggaa gaaatagcgg ggcattcgag
     5941 caacatagcc tttttcgccg cccggtcgtt gattcaaatc aagtgggcgc ggccgatgcg
     6001 ccgccgccgg ggattcgcgg cccgattcgc gatgtaggat tcgggagccc cgaatcggcc
     6061 ctgaaaaccg gctttcaaaa taaagttttt gcataaaagg cggcgaaaaa ccggaatttg
     6121 aaatcctaca aatgcgcgcc gaaaacggca gttttaagta agctggcacg ttgcaaatgt
     6181 aggatttgac cggagcggta aattccgctt gacagcgtca cgctgctggg gtatctttta
     6241 gtcacgctgc ggaattgtag ctgcagtaga tcgaacagcc ggcgcgggcc ttcccgcggc
     6301 cggctttttc tttgcgcgcc gcgcgggcgc cggcgacgcc gggcccatcg atccaaggcg
     6361 ggcgcgcggg cgcggccgac tgccctgcgg ggcggcggcc gccttcattt gccgggacgg
     6421 gtgagatgaa tcggaggagg catgccctgc gtgggcggaa tggacggtac gtcgcggcgg
     6481 cggcgaaggc ggaggaaccg gggctcgagc tcaagcgcgg gcggggcgac cgggtcggcg
     6541 ggcggccgca aatccgccgc aagcgcaagg acgcgctgac cgaatcggag aaggagacct
     6601 tcctgtcgac gttcgccgaa acgtgcaaca tcgcccattc ggcgcggacg gtgggacgca
     6661 aggagcgggt cttccgggac ctcaaaaagc gcgatccggg cttcgccgcc gactggatgg
     6721 cgacccttcg cgagtcctac gacctgctcg aggcggagat ggcccggcgc gcgcggttcg
     6781 ggacccgcaa ggacgtcttc caccagggcc gccgagtcgc gcggatccgg gtgttcaacg
     6841 acgccatggc gctccgcctg ctcagcctcc accgaagcag cgtcgagcgg atgcgggccg
     6901 ccgagtcggc gccccggcgc gacgccaaga cgctgttcga cgagctcgcg gcgcgcgtcg
     6961 ccgagatcaa ggcgaaaagg gcgaaagacg ccgagggagg cgatcatgaa gccgcctgaa
     7021 tggatgtcgc tggccgaaga gatcgccgac ctgggcccgg cggaacgggc gttcgtgctg
     7081 aagacgctca acgcggcgga ggacgcgggc ctgcgctacc tctggcgcct gtgggagcgc
     7141 cgggaccaga gggagccgca gctgccctgg cggacctggc tggtgatggc gggccgaggc
     7201 tatggcaaga cccggatcgg cgcggaatgg gtcaggcgga tggcggcgtc cgatcccacc
     7261 gcctgcatcg cgctggtcgg agcgacgagg gagcaggcgc gggcctggat ggtggagaac
     7321 gacaacggca tcctcggcgt ctgtcccccc gacgagcggc cggcctggga gccctcgctc
     7381 ggacggctga agtggccggg cggcgccacc gccttcgtct attcggcggc cgagcccgag
     7441 agcctgcgcg ggccccagca ccatttcgcc tggtgcgacg agatcgccaa atggccgttc
     7501 ggcccggagg cgtggagcaa cctgatgctg ggcctgaggc gaggatcgct gccgcgggcg
     7561 gtggcgacga cgaccccccg gccggtggca ctggtgcgcc ggctggtcgc ccagggggtc
     7621 gccatgaccc ggggacggac ggtccagaac gcctccaacc tgccgggctt cttcatcgac
     7681 gagataaggc gcgactatgg cagcggccgg ctcggccggc aggagctgga cggcgagctg
     7741 atcgacgacg tggagggaag cctgtggccg cgcgacctga tcgagcggtg ccgggccgtc
     7801 tcgccgccgc ccgagctgcg ccgggtcgtc gtcgcggtgg acccgccggc gtcggcgcag
     7861 ggcacgtgcg ggatcgtcgt gtgcgcgctc ggcggggacg ggctcggcta tgtgctcgcc
     7921 gacgcgaccg tcgtcgggct ccggcccgag ggctgggcgc gcaaggtgga ggccgcggcc
     7981 tcggcctgga acgccgaccg ggtgatcggc gagggcaacc agggcggcgc gatggtcgag
     8041 agcgtgcttc gcggcgccgg ggtcgcgctg ccggtgaagc tggtctacgc ccatccgggc
     8101 aagaccgccc gggcggagcc ggtcgcggcc ctgttcgagg gcgggcgggc gaggttcgcc
     8161 ggcggcttcc ccgagctcga ggaccagctg gccgggatgg tcgcgggcgg cgactacgag
     8221 gggcctggac gctcgcccga ccgcgccgac gcgatggtct gggcgatgac cgagctgatg
     8281 ctgcgccggc gtcgggcgga gccgcggatc cgatcgctgt aggggcgccg ccggaccggc
     8341 tgcgggcccg ggcgcggcct cgaaaaaagc cgtggccccg gggggcgggc taggctaggc
     8401 tgggccgggg agaagaggag gcggatcatg gacttggttc gggtcgcgac gggaatggcg
     8461 gtgctgctcg cgagcggcgc cccggcggcc gccgcgccgc gcaaggacca ggatcccgac
     8521 cgccagatct gcaagtccaa gccggtgatc ggctcgcggc tgaagcgggt ccgcgaatgc
     8581 cacagcgccg ccgaatggga ggatctgaag ctggccgagc agatcggcct cggccgccag
     8641 cagttcaacg gcgccgacgg cctcggcgcc cgggaaagct atctgccgcc gggggcggcg
     8701 gcgcagcagc cgaattgagc cgggccggcc ggagcggcgc gcgggcgcct ggaacgagga
     8761 gattggagat gaagctgatt cggatcgcgg ccggcgcggc gctgatcgcc gccgcctcgg
     8821 cgccgggcgc ggcggcgccc aaggggaagg ccggctacga cccggatcgc cagatctgca
     8881 agtcgagggc cgtggtcggc tcgcgggtga agaggatccg cgaatgcgcg accgcccagc
     8941 aatgggagga catggagctg caggagcgga tggggctgat gcgcaagcag atcaacggcg
     9001 accccggctg cagcggcggc cattccgatt cctgccaccc gatgggagtc aaggactcgc
     9061 cgatctgaac gaggaaaggt caagggaatg cccagcgtga ggatatcggc cgccctggcg
     9121 gggctggccc tgctggcgac ggccttcgcg ccggccgcgg cggcgccgcg caagggcaag
     9181 ccggcctatg atccggaccg gcagatctgc aagtccaagc cggtggtcgg atcgcggctc
     9241 aagcggatcc gcgaatgcca cagcgccgcc gaatgggagg acctggagct gcaggagcgg
     9301 gtcgggctga tgcgcaagca ggtcaacggc gatcccggct gcaacggcgg cgggcagtgc
     9361 ggaatccaga acggcgggcg cgacacgccc tggtgagggt tcgaagggag acgaggatgc
     9421 ggatggtgaa gctcatggcg gtcctggccg tgctggcggc ggcaccggcg gcggcggcgc
     9481 cgaagaaggg cgagccgggc tacgaccccg atcgcgagat ctgcaagtcg aagccggtgg
     9541 tcggctcgcg gctgaagcgg gtgcgcgaat gcgccacggc tgcgcagtgg gaggagatga
     9601 agctccagga gcgccagggg ctgctgcggc ggcagaccaa cggcgacccg tccaacgcgc
     9661 cggtcgcggc cggcgggcgg gacacgccct tctaggcggc cgggcggatc ggaaaggagg
     9721 cggaggatga agcggatcgc gatggcggcg gcgctggtcc tcccgggcgc gatgcccggc
     9781 gccgcaccgg cggcgacgaa gggccgggcg gccgacgacc ccggccgcca gatctgccgg
     9841 tcgaagccgg tggtcggctc gcggctcaag aagatccgcg aatgccacag cgccgccgaa
     9901 tgggaggatc tggagctgca ggagcggatg gggctgaggc gccagcagtt caacggcgct
     9961 cccggctgcc cgcaaccgcc ctgcgtggcg gcgcccggcg gcagggacac gccttggtga
    10021 cgagccggag gagcggagca tgacgagcgc gaggatcatg gcgggcctgg ccctgctggc
    10081 ggccgcctcg atgccgggaa gcccggcggc ggcggcgaag aagggcgcgg gcgcggagga
    10141 tcccgaccgc cagatctgca agtccaggtc ggtgatcggc tcgcggctga agcggatccg
    10201 cgaatgccac agcgccgcgg aatgggagga cctcgagctc caggaacgga tggggctgag
    10261 gcgccagcag ttcaacggcg atccgggctg cgccgaagcg gtcggctgcc accttcggcc
    10321 ggggtcgaag gacagcccgc tctagggggc gcgggcccct cacccacgtc cgtcaacgcc
    10381 agcgaagcga ggcgatgccc ggcggacctc ccggtcccgc cggagcgccg cgccgacccg
    10441 cgcctcgccg atgacggagc aaacggagat tttcgatgaa attctggttc ggacagaagg
    10501 ccgcgcagcc tcaagcgcgg ccgccgctcc atcgcgccta tgcggcgtcg gccgcggccg
    10561 gcgactggcc gcgcggctac gaggcgcagg tgcgcgacgc ctatctcggc aatccggtgg
    10621 cgcagcgcgc ggtccggctg gtcgccgaaa gcgtcgcctg ggcgccggtc tacgcgctcg
    10681 acggggcggc cggggcggcg gcctcggcga aggcggtggg gctggtcggc ccgccgctgc
    10741 tcgactcggc ggcggcgcag ctgctgctcc acggcaacgc cttcgtccag atcctcctcg
    10801 actccgacgg agcgccagcg gagctgttcg ccttgaggcc cgagcgggtg acggtcgagg
    10861 ccgatgccgg cggctggccg gcggcctatg tctatcgggc gggcgaggtg aagacgcggc
    10921 tgccggcgcg cgacgcgctg gggcggccgt cgctgatcca cgtcaaggcg atgcatccgc
    10981 tcgacgacca ttacgggctc ggctgcctgg gcgcggcggc gggggcggtg gcgatccaca
    11041 attcggcgac ccgctggaac aaggccctgc tcgacaatat ggcgcggcct tccggcgcgc
    11101 tcagcctgga cggcgacgag gcgactctgg cgcccgagca atatcagcgg ctgcgcgacg
    11161 agctcgagct ccagttcggc ggcgagggca atgccggccg gccgatgatc ctcgaaggcg
    11221 ggctcaaatg gcagccgatg agcctcactc cggccgacat ggacttcgtc gcgctcaagg
    11281 cggcggcggc gcgggagatc gcgctcgcct tcggagtgcc gccgatgctg ctcgggctgc
    11341 cgggcgattc gacctacgcc aattaccgag aggccaaccg ggcgctgtgg cggctgaccg
    11401 tgctgccgct ggccgagcgg ctgctcggcg ggatcgcggc ggcgcttgcg gcctggtggc
    11461 cggggctgag gctgaggctc gacgtcgacc aggtgaccgc gctcgccgag gaccgggagc
    11521 ggctgtgggc gcaggtcagc tcggccgact ttttgacgcg cgaggagaaa cgcgagatgc
    11581 tgggcttctg acggggccgt ccgcggactg tcccaggggg gagggcggcg atggagactc
    11641 gggcgatcgt cgaggcgctg gtcgaggcga tcaacggcgg gcgggcggag cgggtggcgg
    11701 cggcgatgag cctcgacgcc gtcttcgtcg actcgctcgg ccggcggatc gaggggcgcc
    11761 gggacctgac cgaaggctgg cgcggctatt tcaggctgtt ccccgactat cggatcgagg
    11821 tggacgggct gatcgtcgag ggccccgagg ccctgctcca cggccgcgcc cggggcacgc
    11881 tgcaccgcga cggccacgcc gtcgacggcg gcggcgggga ggtgccggcg gcgtggcggg
    11941 cggtcagcga cgggcgccgg gtggcgctgt ggcaggtcta tgccgacaac aagccggtct
    12001 acgcgctgct cggcatgtag cgggacgccg cgaggc
//
LOCUS       DASVQZ010000277         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_536319, whole genome shotgun sequence.
ACCESSION   DASVQZ010000277 DASVQZ010000000
VERSION     DASVQZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536319"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VF547_07605"
     CDS             complement(<1..1710)
                     /locus_tag="VF547_07605"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8262723.1"
                     /translation="MTWLLRTVSHSAEGREIVRSRRVEGDRLTIGRDPHCDVHLTDLA
                     VALRHAAVERRSGRLEVAAEPGLTVELDGRKTALGKIDLAAGGDILIAGHVLRFMPTP
                     AGSDEIAVSVERTTEGEAKLDKSAERLFTLAPVLPGKRVLAWLLASLVLAAGLAWPIK
                     AYHDRQQRGETFARFHADRLWSSGSLSSAHAGLKDDCTACHVKPFEAVRDAACKSCHA
                     AVHDHADPFRLARSRPDLGRWRRVELAFKEAFDLPPGRCVECHTEHEGRQQMPVAAQR
                     FCADCHADLSRKLPDTRLPDAGDFGRSHPEFRPVLITAWDGARPRLERRSLAGRPIET
                     SNLKFPHAMHLSLTNGVAQMARRLGAAHGFGQALECKDCHDPAPDGARFQPVDMEEDC
                     AMCHSLAFDRAGGTLRTLRHGEPAQVVADLRDFYRGRSPPPPAVLAPAARRRPGDAPD
                     LRNRIQFARGPGSAERAIRAVFSPGGACFDCHQVQAPRAGSLDFRIRPVAFPVRYLHK
                     GWFDHRAHSTQSCSSCHAAGRSNAASDLLLPDLAGCRTCHGGEGSSKAVPSSCAMCHD
                     YHMD"
     gene            complement(1717..2589)
                     /locus_tag="VF547_07610"
     CDS             complement(1717..2589)
                     /locus_tag="VF547_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262724.1"
                     /translation="MAEGIARTDLRHGASHQGFLRHRNFLWAKVALAFSLLALLGYAL
                     ADVQPRRNGGSWYGYTLGTIGILLILWLTLLGLRKRAMTRGRWSLKAWTSAHVYLGLS
                     LAVVATLHTGFQLGLNVHSLAWALMMVVIVSGLYGVAVYAALPPRLGASRSGMTRPQM
                     IDAVAALDRQLRAAAQPLDDDDARIVRLSLEDSPFRAGLWRRLSGRYPRCRTARALAE
                     IRRRAAAAADAQAEALASVVGLLERKSAALARIRRHLRLKALLEIWLYVHVPATFALI
                     AALAAHVVSVFFYW"
     gene            complement(2715..>3191)
                     /locus_tag="VF547_07615"
     CDS             complement(2715..>3191)
                     /locus_tag="VF547_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8262725.1"
                     /translation="DSHEGLSRLNREAGKTYAHLHVPTSCRHCEHPHCMVDCPPNAIH
                     RGPDGEVFIDETCIGCGNCQRNCPYGVIRMEKVPPRKPPLLAWLLAGLGPGPGEPPKK
                     WIDERVSPDTPKKAVKCDMCSGISGGPACVRACPTGAAIRVAPEQFLTVARLERKG"
BASE COUNT          458 a         1056 c         1257 g          420 t
ORIGIN      
        1 gtccatatgg tagtcatggc acatcgcgca ggaggatggc accgctttcg agctcccctc
       61 cccgccgtgg caggtgcggc agccggcgag gtctgggagg agcaggtccg aggcggcgtt
      121 ggaccgcccg gcggcgtggc agctcgagca gctctgggtc gaatgggcgc ggtggtcgaa
      181 ccagcccttg tggagatagc ggacggggaa ggcgaccggc cggatccgga agtcgagcga
      241 gccggcccgc ggcgcctgga cctggtggca gtcgaagcag gcgccgccgg gcgagaagac
      301 ggcccggatc gcgcgctcgg cgctgcccgg cccgcgggcg aactggattc ggttgcgaag
      361 gtcgggagcg tcgccggggc ggcggcgggc ggccggggcc agcaccgccg gcggcggcgg
      421 gcttcggccg cggtagaagt cgcgaaggtc ggcgacgacc tgggccggct cgccgtggcg
      481 cagggtgcgc aaggtgccgc cggcgcggtc gaaggcgagg ctgtggcaca tcgcgcaatc
      541 ctcctccatg tcgaccggct ggaagcgggc gccgtcgggc gccggatcgt ggcagtcctt
      601 gcactcgagc gcctgcccga agccatgggc cgcgcccagc cgccgggcca tctgggcgac
      661 cccgttggtc agcgacaggt gcatggcgtg cgggaatttg aggttgctcg tctcgatcgg
      721 ccgaccggcg agcgaccggc gttccagccg gggccgagcg ccgtcccacg ccgtgatcag
      781 caccggccgg aactcgggat gcgagcggcc gaagtcgccc gcgtcgggaa ggcgggtgtc
      841 ggggagcttg cggctcagat cggcgtggca gtcggcgcag aagcgctggg ccgccaccgg
      901 catctgctgc cggccctcgt gctcggtatg gcattcgacg cagcgccccg gcggcaggtc
      961 gaacgcctcc ttgaaggcga gctcgacccg ccgccaccgg ccgaggtccg gcctcgagcg
     1021 ggcgaggcgg aacggatcgg catggtcgtg gacggcggcg tggcagctct tgcaggcggc
     1081 gtcgcggacc gcctcgaacg gcttgacgtg gcaggcggtg caatcgtcct tgagcccggc
     1141 atgggcggaa gagaggctgc ccgacgacca caagcggtcg gcgtggaagc gggcgaaggt
     1201 ctcgccgcgc tgctggcggt cgtgataagc cttgatcggc caggcgaggc cggcggcgag
     1261 gacgagcgag gcgagcagcc aggccaggac gcgcttgccg ggcaggaccg gcgccagggt
     1321 gaacagccgc tcggcgctct tgtcgagctt cgcctcgccc tcggtggtcc gctcgaccga
     1381 gaccgcgatc tcgtccgatc cggccggagt cggcatgaag cgcaggacat ggccggcgat
     1441 caggatgtcg ccgccggcgg cgaggtcgat cttccccagc gccgtcttgc ggccgtccag
     1501 ctcgacggtg aggcccggct cggcggcgac ctcgagccgg cccgagcgcc gttcgacggc
     1561 ggcgtggcgg agcgccaccg cgaggtcggt caggtggacg tcgcaatggg gatcgcggcc
     1621 gatggtgagc cggtcgccct ccacccggcg ggaccggacg atctcgcggc cttcggcgct
     1681 gtgggagacg gttcgcagaa gccaggtcat tcgccgctac cagtagaaga agacgctgac
     1741 gacgtgggcg gcgagcgcgg cgatcagggc gaaggtggcc ggcacgtgga cgtagagcca
     1801 gatttcgagc agcgccttga ggcgcaggtg ccggcggatc cgggcgagcg cggcggactt
     1861 gcgctcgagc agccccacca cgctcgccag cgcctcggcc tgagcgtccg cggccgccgc
     1921 ggcgcggcgc cggatctcgg cgagcgcccg ggccgtccgg cagcgcggat agcggcccga
     1981 gagccgccgc cagaggccgg cgcggaacgg cgaatcctcg agcgagagcc ggacgatgcg
     2041 ggcgtcgtcg tcgtcgagcg gctgggcggc cgcgcgcagc tggcggtcga gcgccgccac
     2101 cgcgtcgatc atctgcggcc gggtcatgcc ggagcggctg gcgccgagcc ggggcggcag
     2161 agccgcgtag acggcgactc cgtacaggcc cgagacgatg acgaccatca tcagcgccca
     2221 ggcgaggctg tggacgttca agccgagctg gaagccggtg tgaagagtcg cgacgacggc
     2281 cagcgacagg ccgagataga cgtgggcgct ggtccaggcc ttgagcgacc agcggccgcg
     2341 ggtcatcgcc ctcttcctca ggccgagcag ggtcagccag aggatgagca ggatcccgat
     2401 cgtgccgagc gtgtagccgt accagctgcc gccgttgcgc cggggctgga cgtcggccag
     2461 ggcgtagccg agcagggcga ggagggagaa ggcgagcgcg accttcgccc acaggaagtt
     2521 gcggtggcgc aggaagccct ggtggctggc gccgtgccgc aggtcggtgc gggcgattcc
     2581 ttcggccatc tacgtcgccc cgcccaagga cccgtcattg cgaggagcga agcgacgcgg
     2641 caatccagcg cagagtcggt ctccgcccga gcgctggatt gcttcgcttc gctcgcaatg
     2701 acgcagaggg gttgtcatcc cttccgctcc agccgggcga cggtgaggaa ctgctccggc
     2761 gcgacccgga tcgccgcccc ggtcgggcag gctcggacgc aggcggggcc gccggagatg
     2821 ccggagcaca tgtcgcactt caccgccttc ttcggcgtgt cgggcgacac gcgctcgtcg
     2881 atccatttct tcggcggctc gccgggaccg ggcccgaggc cggcgagaag ccaggccagc
     2941 agcggcggct tcctcggcgg caccttctcc atccggatca cgccataggg gcagttcctc
     3001 tggcaattgc cgcagccgat gcaggtctcg tcgatgaaca cctcgccgtc cgggcctcgg
     3061 tggatggcgt tgggcgggca gtcgaccatg cagtgcggat gctcgcaatg gcggcacgag
     3121 gtcggaacgt ggagatgggc gtaggtcttg cccgcctcgc ggttgagcct cgacaggccc
     3181 tcgtggctgt c
//
LOCUS       DASVQZ010000278         6121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_157310, whole genome shotgun sequence.
ACCESSION   DASVQZ010000278 DASVQZ010000000
VERSION     DASVQZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157310"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..995)
                     /locus_tag="VF547_07620"
     CDS             complement(99..995)
                     /locus_tag="VF547_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HEX8262726.1"
                     /translation="MDQARMTAQMETGEGFIAALDQSGGSTPKALKGYGIEEGAWSSD
                     EEMFDLIHRMRSRIITSPAFASGKVIGAILFERTMDGEIDGKPVPQVLKERGVVPFIK
                     IDKGLEAEENGVQLMKPMPDLDALLARAKALGVFGTKERSVINLANPEGIAAAVRQQF
                     EVGRQVLAAGLVPMIEPEINIKSPERAEADQILLEELTKALDSMPGDEKVMLKLSIPA
                     KAGLFQSMVDHPRVLRVVALSGGYARPEACAELAKNPGMIASFSRALLEDLRHQMSGD
                     EFDQSLGGAIDEIYRASTTKTA"
     gene            complement(1107..1514)
                     /locus_tag="VF547_07625"
     CDS             complement(1107..1514)
                     /locus_tag="VF547_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8262727.1"
                     /translation="MIRLAAIDHVVFRVRDIEAALRFYTEVLGATLEKVQAEIGLWQL
                     RAGTSLIDLVPLDGTLGRTGGAGPGEEGRNVDHVCFRVVPWDEAAIVAQLARHGIEAK
                     VARRYGAEGYGPSLYLTDPDGNGLELKGPPAAA"
     gene            complement(1511..2701)
                     /locus_tag="VF547_07630"
     CDS             complement(1511..2701)
                     /locus_tag="VF547_07630"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX8262728.1"
                     /translation="MTSFKTLDDLGDVHGKRALVRVDLNVPMADLEVTDDTRLRATLP
                     TVTELADKGAIVLLLAHFGRPKAPSPEFSLSLVTRAYQAVLGRPVRYIDWEVAEENVA
                     MLRPGDVAILENTRFFGGEERNDPAVAERFARLGDFYVNDAFSAAHRAHASTEAIARL
                     LPAYAGRAMEAELSALQKALGEPERPVAAVVGGAKVSTKLEVLRHLVAKVDHLIIGGG
                     MANTFLAARGVDVGRSLCEHELSATALEILDAAERADCTVHLPYEVVVAKEFAADPPS
                     LRTCNVHEVAADEMILDVGPAAVEALADVLKTCRTLVWNGPLGAFETPPFDRATVQLA
                     RTAAALTQAGQLVSVAGGGDTVAALNQAGAAGDFSFVSTAGGAFLEWMEGKTLPGVKA
                     LERE"
     gene            complement(2732..3742)
                     /gene="gap"
                     /locus_tag="VF547_07635"
     CDS             complement(2732..3742)
                     /gene="gap"
                     /locus_tag="VF547_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX8262729.1"
                     /translation="MATRVAINGFGRIGRLVARALLQKADSGLELVSINDLADAESNA
                     LLFKRDSVHGAWPGEVRADGNDLVIDGRRVKVTAERDPGKLPHGANGVELVLECTGFF
                     TDRDKAGAHLTAGARKVLISAPAKGVDLTVVYGVNHERLEAGHTIVSNASCTTNCLAP
                     VAKVLNDSIGIERGLMTTVHAYTNDQKILDQIHSDKRRARAAAMSMIPTTTGAARAVG
                     EVLPELKGKLDGSAIRVPTPNVSLVDLTFTPKRDTSLDEVNGALRAASESGPLAGILA
                     YSDEPLVSIDYNHNSASSTVDSLETAVLEGKLVRVLSWYDNEWGFSNRMVDTAGAMAR
                     LI"
     gene            complement(3744..5732)
                     /gene="tkt"
                     /locus_tag="VF547_07640"
     CDS             complement(3744..5732)
                     /gene="tkt"
                     /locus_tag="VF547_07640"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX8262730.1"
                     /translation="MTADPRLLANAIRALSMDAVQAANSGHPGMPMGMADVATVLFTE
                     YLRHDPADPAWHDRDRFVLSAGHGSMLIYALLHLTGYAHPTIEEIANFRQLGSPCAGH
                     PENFMLAGVEATTGPLGQGFAMAVGMAVAERHLNAVFGDDLVDHRTWVIAGDGCLMEG
                     VNHEAAGLAGHLGLGRLNVFWDDNGITIDGSTDLSRSEDTLGRYRASGWHTVACDGHD
                     FADIRRAIDEALADPRPSLIACRTIIGWGAPNKQGTAATHGAALGDEEVAAARKQLVW
                     DSEPFDIPEPIQNAWREAGRRSRQDHEDYRARLAASPKRDEFVRRMEGRLPPDFSISA
                     YLEQLLANPQRVATRKASELALEAINAALPETIGGSADLTGSNNTKTKAQKPLTKEDY
                     SGRYLYYGIREFGMAAAMNGMALHGGVIPYGGTFLVFSDYCRPAIRLSALQDARVIYV
                     MTHDSIGLGEDGPTHQPVEHLMSLRAIPNLVVYRPSGAIETAECWTLALAAKGPSVLA
                     LTRQNLLEIRTSVSENLSARGGYRIISSALPRRVILIATGSEVELAIDLADHLESLEI
                     GADVVSMPSTELFDAQPAEYREDILADLSSRDVLRVSIEAGTTFGWERYTGPHGLRIG
                     LDRFGASAPAEHLFKRFGFTVEAIAPQIISALESQGDQ"
     gene            5880..6080
                     /locus_tag="VF547_07645"
     CDS             5880..6080
                     /locus_tag="VF547_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262731.1"
                     /translation="MGQERVEAAIGRIERGLARIERAAARVPTPAEPADGALREAHEA
                     LRGRVREALTQLDGLIAAAERS"
BASE COUNT          899 a         2057 c         2178 g          987 t
ORIGIN      
        1 gagccaggcg ccgaagatcc atcgtccggc aaagccccgg ggatgggcga actgccgcgc
       61 caggtactgg tacaccgggc gcggcagcat ccgcttggtc aggcggtctt ggtcgtcgag
      121 gcccggtaga tctcgtcgat cgcgccgccg agcgactggt cgaactcgtc gccgctcatc
      181 tggtggcgga gatcctccag gagagcgcgg ctgaagcttg cgatcatgcc ggggttcttg
      241 gccagctccg cgcaggcctc gggccgggca tagccgcccg acagcgcaac cacccgaaga
      301 acgcgcggat gatcgaccat cgactggaac aggcccgcct ttgcggggat cgagagcttc
      361 agcatcacct tctcgtcgcc cggcatgctg tccagcgcct tcgtgagctc ctcgagcagg
      421 atctggtcgg cctcggcgcg ttccgggctc ttgatgttga tttccggctc gatcatcggg
      481 accagcccgg cggcgagcac ctgccggccg acctcgaact gctgccggac cgccgcggcg
      541 atgccctcgg ggttcgccag gttgatcacc gagcgctcct tggtgccgaa cacgccgagc
      601 gccttggctc gggcgagcag agcatcgagg tcgggcatcg gcttcatcag ctggacgccg
      661 ttctcctcgg cctcgaggcc cttgtcgatc ttgatgaagg ggacgactcc gcgctccttc
      721 agcacctgcg gcaccggctt gccgtcgatc tcgccgtcca tcgtgcgctc gaacaggatc
      781 gcgccgatca ccttgccgct cgcgaaggcg ggggaggtga tgatcctcga gcgcatccgg
      841 tggatgaggt cgaacatctc ctcgtccgag ctccaggcgc cctcctcgat gccgtatccc
      901 ttgagcgcct tcggagtcga tccgccgctc tggtcgagcg cggcgatgaa cccctcgccc
      961 gtctccatct gcgccgtcat cctcgcctgg tccatctgcg gtcgtccctc atgattcgcg
     1021 atcggtcgcc ggtcccgtat cccagcggga cgcggcgttc aaatgcgggc gcgggccgcc
     1081 cctgccccca acgccccgcg acgggctcag gccgccgccg gcggcccctt cagctcgagg
     1141 ccgttgccgt cgggatcggt gaggtagagg gaggggccgt agccttcggc gccgtagcgc
     1201 cgggcgacct tcgcctcgat gccgtgccgg gcgagctggg cgacgatcgc ggcctcgtcc
     1261 cagggcacga cccggaagca gacgtggtcg acgttgcggc cctcctcgcc cggccccgcg
     1321 ccgcccgtcc ggccgagcgt gccgtcgagc gggacgaggt cgatcagcga ggttccggcg
     1381 cgaagctgcc acaggccgat ctccgcctgg accttctcca gggtggcccc caggacctcg
     1441 gtgtagaagc gaagcgccgc ctcgatgtcg cggacccgga agacgacgtg gtcgatcgcg
     1501 gcgaggcgga tcattcccgc tccagcgcct tcacgcccgg cagcgtcttc ccctccatcc
     1561 actcgaggaa ggccccgccg gccgtcgaca cgaagctgaa gtcaccggcc gctccggcct
     1621 ggttgagcgc cgccaccgtg tcgccgccgc cggccacgga gacgagctgg cccgcctggg
     1681 tgagcgcggc cgcggtgcgg gcgagctgga cggtggcgcg gtcgaacggc ggggtctcga
     1741 acgctccgag cggtccgttc cagaccagcg tgcggcaggt cttgaggacg tcggccagag
     1801 cctcgaccgc cgcgggcccg acgtcgagga tcatctcgtc ggccgcgacc tcgtggacgt
     1861 tgcaggtcct caggctgggc gggtcggcgg cgaattcctt cgccaccacc acctcgtagg
     1921 gcaggtgcac ggtgcaatcc gcccgctcgg cggcatcgag tatctcgagc gcggtggcgc
     1981 tcagctcgtg ctcgcacagc gacctgccga cgtcgacccc gcgcgcggcg aggaaggtgt
     2041 tggccatgcc gccgccgatg atcagatggt cgaccttggc caccaggtgg cgcagcacct
     2101 cgagcttggt cgagaccttg gcgccgccga ccaccgccgc caccggccgc tccggctctc
     2161 ccagcgcttt ctgcaaggcg ctcagctccg cctccatcgc cctgccggca taagcgggca
     2221 gcaggcgggc gatggcttcg gtcgaggcat gggcgcggtg cgcggccgag aaggcgtcgt
     2281 tgacgtagaa atcgccgagc cgcgcgaagc gctcggcgac ggccggatcg ttcctctcct
     2341 cgccgccgaa gaagcgggtg ttctcgagga tggcgacgtc gccgggacgg agcatcgcca
     2401 cattctcctc ggccacctcc cagtcgatgt agcgcaccgg gcggccgagc acggcctgat
     2461 aggcccgggt gaccagcgac agcgagaatt cggggctcgg cgccttcggc cggccgaaat
     2521 gcgcgagcag cagcacgatc gcacccttgt cggcgagctc ggtcaccgtc ggcaaggtgg
     2581 ctcgaagccg ggtgtcgtcg gtcacttcca gatccgccat cggcacgttg aggtcgaccc
     2641 ggacgagcgc ccgcttgccg tgcacgtcgc cgagatcgtc gagggtcttg aatgacgtca
     2701 tggtgtcaaa tccatcgccc cggcgagtcc gtcagatcag ccgggccatc gcgccggcgg
     2761 tgtcgaccat gcggttcgag aagccccatt cgttgtcgta ccaggaaagg actcggacca
     2821 gcttgccctc caggacggcg gtctcgaggc tgtcgacggt cgagctggcc gaattgtggt
     2881 tgtagtcgat cgagaccagc ggctcgtcgc tataggcgag gatgccggcg agcggtccgc
     2941 tttccgaagc ggcgcggagg gcgccgttga cctcgtcgag gctggtgtcg cgcttcggcg
     3001 tgaaggtgag gtcgacgagg ctgacgttcg gcgtcggcac ccggatcgcc gagccgtcga
     3061 gcttgccctt cagttccggc agcacctcgc cgacggctcg ggcggcgccg gtcgtcgtcg
     3121 ggatcatcga catcgccgcg gcgcgggcgc ggcgcttgtc cgaatggatc tggtcgagga
     3181 tcttctggtc gttggtatag gcatggaccg tcgtcatcag gcccctctcg attccgatcg
     3241 aatcgttgag gaccttggcg acgggggcga ggcagttggt cgtgcaggac gcgttggaga
     3301 cgatcgtgtg gccggcctcc agccgctcgt gattgactcc gtagacgacc gtcaggtcga
     3361 ctcccttggc gggggcggag atcaggactt tccttgcgcc cgcagtcaga tgagcgccag
     3421 ccttgtcacg atccgtgaag aatcccgtgc attcgaggac gagctcgacg ccgttggcgc
     3481 catgcggcag cttgcccgga tcgcgttcgg cggtgacctt gacccggcgg ccgtcgatga
     3541 cgaggtcgtt gccgtcggcc cggacctcgc ccggccaggc gccgtggacc gaatcgcgct
     3601 tgaacagaag ggcgttggat tcggcgtcgg cgaggtcgtt gatcgagacc agctcgagcc
     3661 cgctgtccgc cttctggagc agcgcgcggg ccacgaggcg gccgatccgc ccgaatccgt
     3721 tgatcgcaac tctcgtcgcc atgttattga tctccctggg attccaatgc cgagatgatt
     3781 tgcggcgcga tcgcctcgac ggtgaagccg aagcgcttga acagatgctc cgccggcgcg
     3841 gaggcgccga agcggtcgag cccgatgcga aggccgtgcg gccccgtgta gcgctcccat
     3901 ccgaacgtgg ttccggcctc gatcgagacg cgcaggacgt cgcggctcga aaggtcggcg
     3961 aggatgtcct cccgatactc cgccggctgg gcgtcgaaga gctcggtcga gggcatggac
     4021 acgacgtcgg ccccgatctc cagcgactcg agatggtcgg caagatcgat ggcgagctcg
     4081 acctccgatc cggtggcgat caggatgacc cttcggggca gggccgagga gatgatccga
     4141 tagccccccc gcgcgctcag gttctcgctc acgctggtgc ggatctcgag aaggttctgg
     4201 cgcgtgaggg cgaggacgct cgggcccttg gcggccagcg ccagcgtcca gcattcggcg
     4261 gtctcgatcg ctccggacgg gcggtagacg acgaggttgg gaatggcgcg aaggctcatc
     4321 agatgctcga ccggctggtg ggtcgggccg tcctcgccga ggccgatcga atcatgggtc
     4381 atcacgtaga tgacccgggc atcctggagc gccgagagcc ggatggcagg gcggcaataa
     4441 tcggagaaga ccaggaaggt gccgccgtag gggatgacgc cgccgtgcag cgccattccg
     4501 ttcatcgccg cggccattcc gaactcgcgg atgccgtaat agaggtagcg gccggaataa
     4561 tcctccttcg tcagaggctt ctgggccttt gttttggtgt tgttggaacc cgtcaggtcg
     4621 gccgagccgc cgatcgtctc gggcagcgcg gcgttgatcg cctccagggc gagctccgag
     4681 gccttgcggg tcgcgacgcg ctgggggttc gccagcaact gctcgagata ggcggagatg
     4741 gagaaatccg gcggcagccg cccctccatc cggcggacga attcgtcgcg cttcggcgac
     4801 gcggccaggc gggcgcgata atcctcatgg tcctgacggg atcggcgccc cgcctcgcgc
     4861 caggcgttct ggatcggctc ggggatgtcg aacggctcgc tgtcccagac cagctgcttg
     4921 cgcgccgcgg cgacctcctc gtcgccgagc gcggcgccgt gggtggcggc ggtgccctgc
     4981 ttgttgggcg cgccccagcc gatgatcgtc cggcacgcga tcagcgaggg ccggggatcg
     5041 gccagggcct cgtcaatggc ccggcggatg tcggcgaaat cgtggccgtc gcacgcgacc
     5101 gtgtgccagc cgctcgcccg gtagcggccg agcgtgtcct ccgagcggga gaggtcggtc
     5161 gagccgtcga tggtgatccc gttgtcgtcc cagaagacgt tgaggcggcc gaggccgaga
     5221 tgcccggcca ggcccgccgc ctcgtggttg acgccttcca tcaggcagcc gtcgccggcg
     5281 atcacccagg tccggtgatc gaccaaatcg tcgccgaaaa cggcgttcag atgccgctcg
     5341 gcgaccgcca tgcccaccgc catggcgaag ccctggccga gcgggccggt ggtcgcctcg
     5401 accccggcga gcatgaagtt ctcgggatgg ccggcgcagg ggctgccgag ctggcggaaa
     5461 ttggcgatct cctcgatcgt cgggtgggcg tagccggtca ggtggaggag cgcgtagatc
     5521 agcatcgagc cgtggccggc cgagaggacg aagcggtcgc ggtcgtgcca ggccggatcg
     5581 gccgggtcgt gcctcagata ttcggtgaac aggacggtgg cgacgtccgc catccccatc
     5641 ggcatccccg gatggccgga attggcggcc tggaccgcgt ccatcgacag cgcgcgaatg
     5701 gcgttggcga gcagtctcgg atcggcggtc atgcactttc ccaagaggcg gcgacccgca
     5761 cggacgcggg aggagagtcg ggagactccc cgggtcgagc gctctttatc ggcaggggcg
     5821 cgcccgtcaa ccgcgccccg ctgttttcaa agctgcgaag gtcatgctat gcagcggcga
     5881 tggggcaaga gagggtcgaa gcggccatcg gccggatcga gcggggcttg gcgcggatcg
     5941 aacgcgccgc ggcgcgcgtg ccgacgccgg ccgagcccgc cgacggcgcc cttcgcgaag
     6001 cccatgaggc gctgcggggc cgcgtccgcg aggccctcac ccagctcgac gggctgatcg
     6061 ccgcggcgga gcgcagctga tggcgtcggt cgaggtcgag atcgccgccc gcaaatattc
     6121 g
//
LOCUS       DASVQZ010000279         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_732203, whole genome shotgun sequence.
ACCESSION   DASVQZ010000279 DASVQZ010000000
VERSION     DASVQZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732203"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VF547_07650"
     CDS             <1..785
                     /locus_tag="VF547_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8262732.1"
                     /translation="QFLAYVHNAEDQIRGEAATLSFFSNGQYTNPTIIRIAGLGYQKG
                     FGGHFHNDNSLAVFRDIPGLILACPSNGADAVEMLRECVRLALEEQRVIVFVEPIALY
                     MTRDLHEEGDALWTSTYAAPGEARPIRLGEPAVHGDGTDLAIVSYANGYYLSRQAERI
                     LSDQHRVRTRVIDLRWLAPLAEEALLDAVAGCGKVLIVDECRRTGSQSEALMALFAER
                     APQVDCRRITAEDSFIPLGRAATLTLPSRDSIVAAALELVRG"
     gene            778..1821
                     /locus_tag="VF547_07655"
     CDS             778..1821
                     /locus_tag="VF547_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8262733.1"
                     /translation="MAEREAALVVCPGRGTYGKAELGYLKRHHGDKDSLIAGFDRLRA
                     ERGQPTLSELDGAERFSPALHTRGDIASPLIFAASYADFLAVDRSRFDVAAVTGNSMG
                     WYTALAVAGAVGSEQGFRIIDAMGENSQAGERCGQVLLTLVDEEWRELPGLRGQVLAL
                     VQAIEGREGCSLHVSIELGGMIVLAGNEPSLAALLAEAPPTPAREPLRLVNHGPFHTP
                     LMAGSSERALAQLPVDWFRSPSVPMVDGRGHVWRPFATNPTLLRDYTFVAQILQTYDF
                     TRAIQVAVKEHAPDRIILLGPGDTLGGAIAQALIAIEWRGLRSKSDFQQMQAEEPFLL
                     AMGREDQRAVVTG"
     gene            complement(1919..2419)
                     /locus_tag="VF547_07660"
     CDS             complement(1919..2419)
                     /locus_tag="VF547_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8262734.1"
                     /translation="MSVLRPLTAGEIRLLDEAFRGGIAPAMVRLVDGHDDNPLARAAF
                     RNGNTAITLRRTIYFGCHYCPDFARAGPHAQGLFLHEMTHVWQYARLGLARFALLYAR
                     DLCACRFSARAMYRYEEGETRFAGARLEAQAQMVGDYAEAKLAGEAPRMARLARNLRE
                     TGFYGL"
     gene            complement(2416..3411)
                     /locus_tag="VF547_07665"
     CDS             complement(2416..3411)
                     /locus_tag="VF547_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEX8262735.1"
                     /translation="MTDNSLTPEIAERFARIALGHVTREYPHKLDHVMDGPEDVAGPR
                     ELHPIFFGSFDWHSCVHGWWLMLRLRRLFPDLGIAQQVGAMADAMLAPDKVAGELAYL
                     DRAYSGGFERPYGWAWLLALHREAQRHLDRPWAADLEPLARAFADRFQTYLPKLTYPI
                     RVGTHFNTAFAMILALEWAESNDPPLAALIRERARSWYGRDRNCQAWEPGGDEFLSPA
                     LTEALAMRVTHGDGEFRSWFGNFLPDLAAGEPESLFTPATVSDRSDGKIAHLDGLNLS
                     RAWCWRRLAPALEPELAELARRTADEHLAASLPHVAGDYMGEHWLATFALLALEG"
     gene            complement(3501..4451)
                     /locus_tag="VF547_07670"
     CDS             complement(3501..4451)
                     /locus_tag="VF547_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEX8262736.1"
                     /translation="MITLHHLYALAGLMFAAFAILSALDRSNQKRLGNAAFWGLMALS
                     LLAGDRIGDFGNGLLVLGLAALASLGLIGRSKPPTSSDEQRESFSARYGNRLFLPALI
                     IPAIAFVGTLLYTYTPLKESGLFEAKRETYVLLGLGVLTALAVIFAWLRPPALAPLQE
                     GRRLIDAIGWAAVLPQMLAALGVLFAAAGVGAVIGEIARAVIPEGSVFLTVAVFALGM
                     ALFTMIMGNAFAAFPVMAAAIGVPLLIQGYGGNPAVVGAIGMLAGFCGTLMTPMAANF
                     NLVPAALLELKDRNGVVKAQVGTALPLLAINILFIWIFAF"
     gene            complement(4448..>5077)
                     /locus_tag="VF547_07675"
     CDS             complement(4448..>5077)
                     /locus_tag="VF547_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 family protein"
                     /protein_id="HEX8262737.1"
                     /translation="NDNRIIAIVWIVLPVIGLLERFGLQQRAAAVIRGFRSATAGRLL
                     ILYLLYRQIISAIGLHSTAGHPQTVRPLLAPMALAAAEKQHGELDEATSEKVKAYSAA
                     TDNVGLFFGEDIFFAIASIVLIQQTLAAYGIVLAPIELGLWAIPTAIAAFLVHGTRLL
                     ILDRSLSSPGRGGGPAKPVEGARLSAASESGPLHHPSDGPPPRSGEEDR"
BASE COUNT          805 a         1708 c         1842 g          722 t
ORIGIN      
        1 tccagttcct cgcctatgtc cacaatgccg aggaccagat ccgcggcgag gcggcgacgc
       61 taagcttctt ctcgaacggc cagtacacca acccgacgat catccggatc gccggcctcg
      121 gctaccagaa gggcttcggc ggccatttcc acaacgacaa cagcctcgcc gtcttccgcg
      181 acattccggg gctgatcctg gcttgcccga gcaacggcgc cgacgccgtg gagatgcttc
      241 gcgaatgcgt ccgcctcgcg ctcgaggagc aaagggtgat cgtcttcgtc gagccgatcg
      301 ccctctacat gacccgcgac ctgcacgagg aaggcgatgc cttgtggacg tcgacctacg
      361 cggcgcccgg cgaggccagg ccgatccggc tcggcgagcc ggcagtccat ggcgacggca
      421 cggacctggc gatcgtcagc tacgccaacg gctattacct ctcgcgccag gcggagcgga
      481 tcctcagcga ccagcatcgc gtcaggacgc gggtgatcga cctgcgctgg ctggcgccgc
      541 tcgccgagga ggccttgctc gacgccgtcg cgggctgcgg caaggtcctg atcgtcgacg
      601 aatgccgccg gaccggctcg cagagcgagg ccctgatggc gctgttcgcc gagcgcgcgc
      661 cgcaggtcga ctgccgccgg atcaccgccg aggattcgtt catcccgttg ggccgggccg
      721 cgaccctgac cctgccgagc cgggattcga tcgtcgccgc ggcgctggag ctggtgcgtg
      781 gctgagcgcg aggcggcgct ggtcgtctgc ccgggccggg ggacctacgg caaggcggag
      841 ctcggctatc tgaagcgcca ccatggcgac aaggactcgc tgatcgccgg gttcgaccgc
      901 ctgcgcgccg agcgcggcca gccgacgctg tccgagctcg acggggccga gcgcttcagc
      961 ccggcgcttc acacccgcgg cgacatcgcc tcgccgctca tcttcgccgc ctcctacgcg
     1021 gatttcctcg ccgtcgaccg ctcgcgcttc gacgtcgccg cggtcaccgg caattcgatg
     1081 gggtggtaca ccgccctggc ggtcgccgga gcggtcggca gcgagcaagg cttccggatc
     1141 atcgacgcga tgggcgagaa cagccaggcc ggggagcgct gcggccaggt gctcctcacc
     1201 ctggtcgacg aggagtggcg cgagcttccc ggccttcgcg gccaggtgct ggcccttgtc
     1261 caggcgatcg aggggcgcga gggctgcagc ctccacgtct cgatcgagct cggcggcatg
     1321 atcgtcctcg ccggcaacga gcccagcctc gcggccctcc tcgccgaagc tccgccgacg
     1381 ccggcccggg agccgctccg gctcgtcaat cacggcccct tccacacgcc gctcatggcc
     1441 ggttcctcgg agcgcgcgct ggcgcagctt cccgtcgact ggttccgctc gcccagtgtc
     1501 ccgatggtcg acgggcgagg ccatgtctgg cggccgttcg cgacgaaccc caccctcctt
     1561 cgcgactaca ccttcgtcgc ccagatcctc cagacctacg atttcacccg tgcgatccag
     1621 gtcgcggtca aggagcacgc gccggaccgg atcatcctgc tcggccccgg cgacacgctc
     1681 ggcggcgcca tcgcccaggc gctgatcgcg atcgagtggc gcggccttcg ctcgaagagc
     1741 gacttccagc agatgcaggc ggaggagccc ttcctgctcg cgatgggccg cgaggaccag
     1801 cgggcggtgg tgacgggatg agggccttca ttgggaggag cgaagcgacg cggcaatcca
     1861 gcgccgcctc ggaagctcct ctggattgct tcgcttcgct cgcaatgacg gctgaaggtc
     1921 agagcccgta gaagcccgtt tcccgcaggt tgcgggccag ccgggccatg cgcggcgcct
     1981 ctccggccag cttcgcctcg gcataatcgc cgaccatctg cgcctgcgct tccagccgcg
     2041 cgccggcgaa gcgggtctcg ccttcctcgt accggtacat cgcgcgggcg ctgaaacggc
     2101 aggcgcacag gtcgcgggcg taaaggaggg cgaagcgggc caggccgagc cgcgcatatt
     2161 gccagacgtg ggtcatctcg tggaggaaca ggccctgcgc gtgcgggccg gcccgcgcga
     2221 aatccgggca ataatggcag ccgaaataga tcgtccggcg aagggtgatg gcggtgttgc
     2281 cgttgcggaa ggcggcgcgc gccaggggat tgtcgtcatg gccgtcgacg agacggacca
     2341 tcgccggcgc gatcccgccc cggaaggctt cgtccagcag ccggatctcc ccggcggtga
     2401 ggggccgcaa gacgctcatc cctccagcgc cagcagggcg aaggtggcga gccagtgctc
     2461 gcccatatag tcgccggcga cgtgggggag gctggcggcg agatgctcgt cggcggttcg
     2521 gcgggcgagc tcggccagct cgggctccag agcgggagcg agccgccgcc agcaccaggc
     2581 gcggctgagg ttgagcccgt cgagatgggc gatcttgccg tccgagcggt cggagacggt
     2641 ggccggcgtg aacaggctct ccggctcgcc ggcggcgagg tcgggaagga aattgccgaa
     2701 ccagctgcgg aactcgccgt cgccatgggt cacccgcatc gccagcgcct cggtcagggc
     2761 gggggagagg aactcgtcgc cgccgggctc ccaggcctgg cagtttcgat cccggccgta
     2821 ccagctgcgc gcccgctctc ggatcagggc ggcgagcggg gggtcgttcg actccgccca
     2881 ttcgagcgcg aggatcatcg cgaaggcggt gttgaagtgg gtcccgacgc ggatcggata
     2941 ggtcagcttg ggcagatagg tttggaagcg gtcggcgaag gcgcgggcga gcggctcgag
     3001 gtcggcggcc caagggcggt ccagatggcg ctgcgcctcg cggtggaggg cgagcagcca
     3061 ggcccagccg tagggccgct cgaagccgcc cgaataggcg cggtcgagat aggcgagctc
     3121 gccggccacc ttgtccggcg cgagcattgc gtcggccatt gcacccactt gttgcgcgat
     3181 tccaagatcg gggaagaggc ggcgcagccg gagcatcagc caccagccgt ggacgcagct
     3241 gtgccagtcg aagctgccga agaagatcgg gtgaagctcg cgcgggccgg cgacgtcctc
     3301 gggcccgtcc atcacatggt cgagcttgtg cggatattcg cgggtgacgt ggccgagcgc
     3361 gatccgggcg aagcgctcgg cgatctcggg ggtgaggctg ttgtccgtca ttgcgagcga
     3421 agcgaagcaa tccagtctgg gcggcggtga aggacgccga ggctggattg ccgcgtcgct
     3481 ccgctcctcg caatgacgga ctagaaggcg aaaatccaga tgaagaggat gttgatcgcg
     3541 agcaggggca gcgccgtgcc gacctgcgcc ttgaccacgc cgttgcggtc cttgagctcg
     3601 agcagcgccg ccgggaccag gttgaagttg gccgccatcg gggtcatcag ggtgccgcag
     3661 aagccggcga gcattccgat cgcgccgacc accgccggat tcccgccata gccctggatg
     3721 agcaagggca ccccgatcgc cgcggccatc accgggaagg cggcgaaggc gttgcccatg
     3781 atcatcgtga acagcgccat tcccagcgcg aagacggcga cggtcaggaa gacgctgcct
     3841 tcggggatca ccgcccgggc gatctcgccg atgaccgcgc ccacgcccgc cgcggcgaac
     3901 aggaccccga gcgcggcgag catctgcggc agaaccgccg cccagccgat cgcgtcgatc
     3961 agccgccgtc cctcctggag cggcgccagg gcgggcggcc ggagccaggc gaagatcacc
     4021 gccaaagcgg tcagaacgcc gaggccgagc aggacgtagg tctcgcgctt ggcctcgaac
     4081 aggcccgatt ccttgagcgg ggtgtaggtg tagaggaggg tgccgacgaa ggcgatcgcg
     4141 gggatgatca gggccggcag gaacagccgg ttgccgtagc gggccgagaa ggactcccgc
     4201 tgctcgtccg agctggtcgg cggcttcgag cgcccgatca ggccgaggct ggcgagcgcg
     4261 gcgaggccga ggacgagcag gccgttgccg aagtcgccga tccggtcgcc ggcgagcagg
     4321 ctcagcgcca tcaggcccca gaaggcggcg ttgccgaggc gcttttggtt cgagcggtcg
     4381 agcgcgctca ggatcgcgaa ggccgcgaac atgaggccgg cgagcgcgta gagatggtgg
     4441 agggtgatca tctatcttcc tccccggaac ggggaggggg accatccgaa ggatggtgga
     4501 gggggcccga ctccgaagcg gcggagaggc gggccccctc caccggcttc gccggtcccc
     4561 ctccccgtcc cggggaggaa agcgaccggt ccagaatcag cagccttgtc ccgtgcacca
     4621 ggaacgcggc gatcgccgtc gggatcgccc acaggccgag ctcgatcggc gcgagcacga
     4681 tgccgtaggc ggcgagggtc tgctggatga ggacgatcga ggcgatggcg aagaagatgt
     4741 cctcgccgaa gaagaggccg acgttgtcgg tcgccgcgga ataggccttc accttctccg
     4801 acgtcgcctc gtcgagctcg ccatgctgct tctccgcggc ggcgagcgcc atcggcgcga
     4861 gcagcggacg gaccgtttgc gggtggccgg cggtgctgtg cagcccgatc gccgagatga
     4921 tctggcggta gagcagatag aggatcagga gccgcccggc cgtcgcgctc cggaagccgc
     4981 ggatgacggc cgcggcgcgc tgctggagcc cgaaccgctc gaggaggccg atcaccggga
     5041 gcacgatcca gacgatcgcg atgatccggt tgtcgtt
//
LOCUS       DASVQZ010000280         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_732501, whole genome shotgun sequence.
ACCESSION   DASVQZ010000280 DASVQZ010000000
VERSION     DASVQZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732501"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="ruvB"
                     /locus_tag="VF547_07680"
     CDS             <1..924
                     /gene="ruvB"
                     /locus_tag="VF547_07680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8262738.1"
                     /translation="RENLRVFIEAAKRRGDPLDHVLFFGPPGLGKTTLAQIVARELGV
                     GFRATSGPVIAKSGDLAAILTNLEDGDVLFIDEIHRLAPAVEEILYPAMEDRVLDLMI
                     GEGPSARSVRIDLPKFTLVGATTRQGLITTPLRDRFGIPVRLTFYSVEELEQVVRRAG
                     RLLGLALTDDGAREVARRSRGTPRIAGRLLRRVRDFAQSASEAEVDAKVADSALNRME
                     VDHLGLDAMDRRYLHMIADIYKGGPVGVETLAAGLSEPRDTIEEVIEPFLIQLGLIAR
                     TARGRCLNGPGWTHLGLPQPKSPQGGLFEAQ"
     gene            936..>1860
                     /locus_tag="VF547_07685"
     CDS             936..>1860
                     /locus_tag="VF547_07685"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX8262739.1"
                     /translation="MFRRIAIAVALAATPAAAQEPAVAPDYRQETAWLCLPGREDACG
                     RPLPTTALNANGYGSVGQVRPDPGAPIDCFYVYPTISRDATLNSDLTPGPEEQAVAAI
                     QAARFGSVCRVYAPMYRQVTLAGLAAAFAGGGRGGDQQLAYGDVLAAWRHYIGDRNRR
                     RPFVLIGHSQGTVHLSRLLAEEIEGSEAAGRMLSALLIGFNVEIPEGQVVGGSFRQTP
                     LCTRTGQTGCVVSYVSFRATNPPPAGALFGRAARPGMTVGCTNPAALGSRGSAPLDSY
                     WYAGPSITSTQNPTQWSAQGEAPTPFLRTEGL"
BASE COUNT          266 a          677 c          639 g          278 t
ORIGIN      
        1 cgggagaatc tgcgggtgtt catcgaggcg gcgaagcggc gcggcgatcc gctcgaccat
       61 gtcctcttct tcggcccgcc cggcctcggc aagacgacgt tggcgcagat cgtggcgcgc
      121 gaactcggcg tcggcttccg cgctacgtcg ggtccggtca tcgccaagtc gggcgacctc
      181 gccgcgatcc tcaccaacct cgaggacggc gacgtcctct tcatcgacga gatccaccgg
      241 ctcgctccgg cggtcgagga gatactctat cccgccatgg aggatcgcgt cctcgacctg
      301 atgatcggcg aggggccctc cgcccgttcg gtacggatcg atcttccgaa gttcaccttg
      361 gtgggcgcca ccacccggca gggcctgatc acgacgccgc tgcgcgaccg gttcggaatc
      421 ccggttcgcc tcaccttcta cagcgtggaa gagctggagc aggtcgtccg ccgcgccggg
      481 cggctgctcg gcctcgccct caccgacgac ggcgcgcggg aagtggcgcg gcgctcgcgc
      541 gggacgccgc ggatcgcggg ccggctgctg cgccgggtcc gcgacttcgc tcagtccgcc
      601 agcgaggccg aggtcgacgc gaaggtggcc gacagcgcgc tcaaccggat ggaggtcgac
      661 catctcggcc tcgatgccat ggaccggcgc tacctccaca tgatcgccga catctacaaa
      721 ggcggaccgg tcggggtgga aactctggcc gcggggctca gcgagccgcg cgacacgatc
      781 gaggaggtga tcgaaccctt cctcatccag ctcggcctga tcgcccgcac cgcccggggc
      841 cgctgcctca acggccccgg ctggacccat ctcggcctgc cccagcccaa aagcccccag
      901 ggcggcctgt tcgaagcgca atgaggggag gaaggatgtt tcgccggatt gccatcgcgg
      961 tggcgctcgc tgccacgccg gctgccgccc aggaaccggc ggttgcgccg gattatcggc
     1021 aggaaaccgc ctggctctgc ctccctgggc gcgaggatgc gtgcgggcgg ccgctgccga
     1081 cgacggcgct caacgccaac ggctacggct cggtcggaca ggtccggccc gatccagggg
     1141 cgccgatcga ctgcttctac gtctatccca cgatatcgcg tgacgcgacg ctgaactcgg
     1201 acctgacgcc ggggccggag gagcaggcgg tcgccgccat ccaggcagcg cgcttcggga
     1261 gcgtctgccg ggtctatgcg ccgatgtacc ggcaggtgac tctggccggc ttggcggcgg
     1321 cgttcgccgg cggcggccgc ggcggggacc agcagctcgc ttatggcgac gtgctcgccg
     1381 cctggcgcca ctatatcggc gaccgcaacc gccgtcgccc gttcgtgctg atcggccaca
     1441 gccagggcac cgttcatctc agccggctcc tcgccgagga gatcgagggc agcgaagcgg
     1501 ccgggcgtat gctctccgcg ctcctgatcg gcttcaacgt cgaaattccg gaggggcagg
     1561 tggtcggcgg cagcttccgg cagacgccgc tctgcacccg tacgggccag accggctgcg
     1621 tcgtcagcta cgtctcgttc cgcgccacca acccgccgcc tgccggcgcc ctgttcggcc
     1681 gcgcggcgcg gccggggatg accgtcggct gcacaaatcc ggcggcgctg ggcagccgcg
     1741 gctccgctcc gctcgacagc tactggtatg ccgggccttc gatcaccagc actcagaacc
     1801 cgacccagtg gtccgctcag ggcgaggcgc cgacgccgtt ccttcgcacc gaagggctca
//
LOCUS       DASVQZ010000281         4875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_923436, whole genome shotgun sequence.
ACCESSION   DASVQZ010000281 DASVQZ010000000
VERSION     DASVQZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923436"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..178
                     /locus_tag="VF547_07690"
     CDS             50..178
                     /locus_tag="VF547_07690"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX8262740.1"
                     /translation="MWLESLAFARQRGFRSKERNAILSIVTEHRQAMLEAWHEHFG"
     gene            162..437
                     /locus_tag="VF547_07695"
     CDS             162..437
                     /locus_tag="VF547_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX8262741.1"
                     /translation="MNISARVGDERVADVRCDAERLIVDLADGRTIAVPLAWYPRLLN
                     ATPAQRANWERAGGGFGIHWPDVDEDLSVAGLLDGTPATGRARRPPS"
     gene            606..935
                     /locus_tag="VF547_07700"
     CDS             606..935
                     /locus_tag="VF547_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262742.1"
                     /translation="MALLWILLYAPAVVLLLRVFTSRDPGWVRLLGAVAAAASVALPL
                     GAQAAGWDLSAGLGVTVLGAAASYLLVWSAFSRDGAAVRLACAAVAVAGAFPLLLLYL
                     FATHYTE"
     gene            complement(984..1364)
                     /locus_tag="VF547_07705"
     CDS             complement(984..1364)
                     /locus_tag="VF547_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262743.1"
                     /translation="MVPLEAQDGAAAYTRRLRSLLERPVQRRPRHARLFGTENGTFQH
                     YPLTQLSAVPRNEKSDSIQFPAVPSPERGEKCILSLFSPDLLGQSRLVGDGDGRRRSR
                     RGRLEDRRGGGEEEEKRQGRLLLY"
     gene            1594..2172
                     /locus_tag="VF547_07710"
     CDS             1594..2172
                     /locus_tag="VF547_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HEX8262744.1"
                     /translation="MRLAFAALVLPMMLSAPLGAQPAAPAVAAPVELSRIPGVTIRYY
                     DVAGSTPGKILAAMRSGRPADPAGKVSPSASRWSIGIDIQKATTGARCRVVRATALFR
                     AEVDFPRLVVTARAKDEEEPGEEKLDKFLKRWNEHLATLDRQQAASLRSIHEQLPEVE
                     RAAMAGGCDGARAAAERVIAQIRERSSSPAGR"
     gene            2365..2685
                     /locus_tag="VF547_07715"
     CDS             2365..2685
                     /locus_tag="VF547_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8262745.1"
                     /translation="MAAVSIVVVDTAEFRRWFAGVRDRRAKGRILARLRRLSLGNFGD
                     ARSLGGGLHELRIDYGPGYRVYFARHGGALVLLALGGDKSRQKIDIARAREVAAAWEP
                     ENGH"
     gene            2675..2914
                     /locus_tag="VF547_07720"
     CDS             2675..2914
                     /locus_tag="VF547_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX8262746.1"
                     /translation="MGIETRAWDAADHLDSKETILAYLEAAFEDGDPELIAAALNDIA
                     RAKGLPHDALTAQSDIGSVMVTLKALGLELTAKAA"
     gene            3410..3958
                     /locus_tag="VF547_07725"
     CDS             3410..3958
                     /locus_tag="VF547_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262747.1"
                     /translation="MRRALLASAAALALLSAGGAPATSLGPTPPHDPKLACKAIKQLV
                     ADLNSGRLRDPESWGAGPTFFTDGLGEVEDSEEQAFLHAMRRSEGRPDAKPIELRDVR
                     IVHKDKEDSVYLVLLDRESWHEKRLVSDGMLGMEEIDDPHYATDTGFWLARFMSNDLT
                     DFREAPEMNALWLETPRLKGCH"
     gene            complement(4047..>4875)
                     /locus_tag="VF547_07730"
     CDS             complement(4047..>4875)
                     /locus_tag="VF547_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262748.1"
                     /translation="RLARERIRTTAEYAMFLPILVTTPIAALLILVHLWNLLGGEQWG
                     KLAESLPGIVTFASLAAVPAVASYFPRRRRQKRGLVEPPPGRAGRFELACVGLGALTA
                     LAGLVGTHVAFIRMIDRSYFSNKLEVQHLECHLLEPRPFVDVVLKNHASVATPVDLGA
                     LSLTTGRMKKDANGEWTDESADPWRIPMRMGEDGYGIPALIQPGAVGIVRGWPVVPLP
                     FAPEAGSPCLLSGTVYVGPGGDSHIGVRVTGDLGEVSWPALPARPAAQGDKPAPAGR"
BASE COUNT          762 a         1706 c         1666 g          741 t
ORIGIN      
        1 ggacgagccg ccgcacgtcc atgtcgacaa agcggaagcg acgggcaagg tgtggctgga
       61 gtcgttagcc ttcgcccggc agcgcgggtt ccggtcgaag gaacgcaatg ctatcctgag
      121 catcgtgact gagcatcggc aggcgatgct ggaggcctgg catgaacatt tcggctagag
      181 tgggcgacga gcgggtggcg gacgtgcgct gcgatgccga gcggctgatc gtcgacctcg
      241 ccgacggacg caccatcgcc gtgccgctcg cctggtatcc ccggctgctc aacgccaccc
      301 ccgcccagcg cgccaactgg gagcgggccg gcggcggctt cggcatccac tggccggacg
      361 tggacgagga tctcagcgtc gccggcctcc tcgacggaac accggcgacc gggagggcac
      421 gccgcccgcc gtcctaaacc gcccgcgaga atcgtcgccg cactttgtga gcccgaagat
      481 ggggattgaa acgagccgcg gggaaggtcc ggtgccaggc acgtgccaga tacgtgcctg
      541 gcacccagag catggccggt gccagttacc agggaaaaag ccgatcatgc tataggccgc
      601 cctcgatggc cctcctctgg atcctccttt atgcgccggc ggtggtgctg ctcttgcggg
      661 tcttcacctc gagggatccg ggctgggttc gcctgctcgg cgccgtggcc gccgccgcat
      721 cggtggcgct gccgctgggg gcccaggccg cggggtggga tctctccgcg ggcctaggcg
      781 tgacggtgct cggcgccgcc gcgtcctacc tgctcgtctg gtcggcgttc agccgcgacg
      841 gcgccgccgt ccggctcgcc tgcgcggccg tggccgtcgc cggggcgttc ccgctccttc
      901 tgctttacct gttcgcgacc cactacaccg agtagctcga aggcggccgc ttgccatcag
      961 ccccctccca ctgtattata tacctaatac agcaggaggc gcccctgccg cttctcttcc
     1021 tcctcgccgc cgccgcggcg gtcctccagc cggcctcggc ggcttcgtcg ccggccgtcg
     1081 ccgtccccga ccagccggct ctgaccgagc agatccgggg aaaacagtga cagtatacat
     1141 ttttcgcccc gctccggcga agggacagcg ggaaattgta tactgtcact cttttcgttc
     1201 ctagggaccg cggagagttg cgtaaggggg tagtgctgga aggtcccgtt ctccgtcccg
     1261 aacagtcgcg catggcgcgg gcggcgctga actggtcgct cgagcaggct gcggaggcgg
     1321 cgggtgtaag ccgccgcacc gtcctgcgct tcgagcggaa ccatcgcgac atcaagcccg
     1381 agctgatcca ggccctccgc cgcgccttcg aggatgccgg ggtatcgacg aagcgggccg
     1441 gatcggcgtt acggcacccg ctaaacccgc atgtctctga ttgtctcctt gaaagggcct
     1501 cgagtgcatt cttcgtggtc cctatcgggg accgacgaag gaagccgccc ggccgccttg
     1561 ccttcgctgc cggaatgccg gatcatccgc ggcatgcgcc ttgccttcgc tgccctggtc
     1621 ctgcccatga tgctgtccgc gccgctcggc gctcagccgg cggcgccggc cgttgccgcg
     1681 ccggtcgaac tttcgcggat acccggagtg acgatccgat attacgacgt cgccgggagc
     1741 acgcccggga agatcctcgc cgcgatgagg agcgggcgcc cggcggaccc ggcgggcaag
     1801 gtctcgccgt ccgcgtcgcg ctggtcgatc gggatcgaca tccagaaggc gacgacgggg
     1861 gcccggtgcc gcgtcgttcg cgccaccgcc ctgttccggg ccgaggtgga tttcccccgc
     1921 ctcgtcgtca cggccagggc gaaggacgag gaggagccgg gcgaggagaa gctcgacaag
     1981 ttcctgaagc gctggaacga gcatctcgcg accctcgacc gccagcaggc ggcctccctg
     2041 cgctcgatcc acgagcagct ccccgaggtg gagcgggcgg ccatggccgg cggctgcgac
     2101 ggcgcgcggg cggcggcgga acgggtcatc gcccagatcc gggagcggtc gtcgtccccc
     2161 gccggccgat agcggtccgc tcgctatccg gaaaatgccc gagggcgtcc tcggccggcc
     2221 ccccacagcg acgaggagcg cgccaaggcg gcggtctccg caaagaaagc gtgacagtat
     2281 acaatttcct tggcgagcat ttgctattcg agtgaagctg tatccgattt tggtgaatgt
     2341 cggacgcacc tttagattcg gccgatggcc gccgtatcga ttgtcgtcgt cgacaccgcc
     2401 gagttccgcc gctggttcgc cggtgtccgc gaccgccggg ccaagggccg aattctcgcg
     2461 cggctccgcc gcctgtcgct cggcaatttc ggcgacgcgc ggtcgctcgg cggcggcctg
     2521 cacgaattac ggatcgatta cggccccggt tatcgtgtct atttcgcgcg ccatggcggg
     2581 gcgctcgtcc tgctcgccct gggaggtgac aaatcacgcc agaaaatcga tattgcccga
     2641 gcgcgcgagg tcgccgcggc atgggagccg gaaaatgggc attgaaacgc gggcctggga
     2701 cgccgcggac catctcgaca gcaaggagac catcctcgcc tatctcgaag cggccttcga
     2761 ggacggcgat ccggagctga tcgccgcggc cctcaacgac atcgcacggg ccaagggact
     2821 cccccacgat gcgctgaccg cgcaaagcga tatcggctcc gtgatggtga ccctgaaggc
     2881 gctggggctc gagctcaccg caaaagccgc gtgaaatcgg cgacggcacg tcgcaagact
     2941 gggatgtcgt cgggcaagcg ggaccagccc ccgcggacgc cgccgccagg ccatgagcga
     3001 ccctcactcg cgccaataat catcgcgctc cgagccgtcg aaaccggccg gcggggaggc
     3061 agcagcgctt gaaagctttt cccggagccg caggggcagg ggtccttgcg gccgagcttc
     3121 tcgaccagct ccttctcgcc gccccgcacg aagcgcaggc cgcgcttcac ccgggcctcg
     3181 gaaggatagc ccctacgccg cttggagatc acctcgaaag ccggggaggt cgtcgcgatc
     3241 gtcggtcttc atggcggcct cctctcggct gggaacggcg ggacgcggcc gctagccccg
     3301 gccgcggacg aagtaaagcc gtcgccccgc ggcgatgacg ctcctcccac gccccgcccg
     3361 aagcggcggc gagcttgtct tcgcagccgc ggcggcagat ggtgcgggca tgcgccgagc
     3421 cctcctcgct tccgccgccg ccctcgccct gctgtctgcc ggcggcgcgc cggcgacctc
     3481 gctcggcccg actccgccgc acgatccgaa gctcgcgtgc aaggcgatca agcagctcgt
     3541 cgccgacctc aattcgggac ggctgaggga cccggaatcc tggggcgccg gcccgacctt
     3601 cttcaccgac gggctcggcg aggtcgagga cagcgaggag caggccttcc tccacgcgat
     3661 gcgccggtcg gaaggcaggc cggacgccaa gccgatcgag ctgcgcgacg tccgcatcgt
     3721 ccacaaggac aaggaggact ccgtctacct ggtcctgctg gaccgcgaat cctggcacga
     3781 gaagcggctg gtgtccgacg ggatgctcgg catggaggag attgacgacc cgcattacgc
     3841 caccgacacc gggttctggc tggcccggtt catgtcgaac gacctgaccg atttccgcga
     3901 ggccccggaa atgaacgcgc tctggctcga gacaccccgg ctgaagggct gccattgacg
     3961 gcgggccgcg gtccgggaag cgcaaccgtc cgccccgtcg accgagactg ggtccgattg
     4021 gtgaaggccc ggcgccccca gcccggctac cgcccggccg gagccggctt gtcgccctga
     4081 gcggcgggac gtgccggaag cgccggccag gacacctcgc cgaggtcccc tgtcaccctc
     4141 acgccgatgt gggagtcgcc gcccggcccg acatagaccg tcccggagag aaggcagggg
     4201 gaccccgctt cgggcgcgaa gggcagggga acgaccggcc accccctcac gatcccgacc
     4261 gcgccgggct gaatcagcgc cggaattccg tagccgtctt cgcccatgcg catcggaatc
     4321 cgccacggat ccgcgctttc gtcggtccac tcgccgttcg cgtccttctt catccggccg
     4381 gtcgtcaggg agagcgcgcc aaggtcgacg ggcgtcgcga cggaagcgtg gttcttcagc
     4441 acgacgtcga cgaacggccg cggctcgagc aggtgacatt cgagatgctg cacctccagc
     4501 ttgttgctga agtagcttcg gtcgatcatc cggatgaagg cgacgtgggt gccgacgagg
     4561 ccggccagcg cggtgagggc gccgagcccg acgcatgcga gctcgaagcg tcccgctcgc
     4621 ccgggcggcg gctcgacgag gccgcgcttc tgccgccgcc ggcgtggaaa atagctggcg
     4681 accgccggaa ccgcggcgag cgacgcgaag gtgacgatcc cgggaagcga ctcggccagc
     4741 ttgccccatt gctcgccgcc caagagattc cacagatgga cgaggatcag cagcgcggcg
     4801 atcggagtcg tgaccaggat cggcaggaac atcgcatatt cggcggtcgt ccggatccgc
     4861 tcccgcgcga gccgg
//
LOCUS       DASVQZ010000282         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_732968, whole genome shotgun sequence.
ACCESSION   DASVQZ010000282 DASVQZ010000000
VERSION     DASVQZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732968"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..540)
                     /locus_tag="VF547_07735"
     CDS             complement(340..540)
                     /locus_tag="VF547_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEX8262749.1"
                     /translation="MLMSNKGREARLHYMPGTFRILTPGDHVRCAATGQPIPLEDLRY
                     WSVARQEAYSSAELSVKAEGRG"
     gene            complement(627..2018)
                     /gene="xseA"
                     /locus_tag="VF547_07740"
     CDS             complement(627..2018)
                     /gene="xseA"
                     /locus_tag="VF547_07740"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX8262750.1"
                     /translation="MDDFPPAESGLVATPSPGDNSEPYSVSELAMRLKRTVEDAFGFV
                     RVRGEISGWKRAASGHCYLCLKDDKAVLDGVMWKATAAALPFRPEDGIEVVATGKLTT
                     YPGRSRYQIIIERLELAGQGALMALLDKRRRALAAEGLFDAARKRRLPFQPRVIGVVT
                     SPTGAVIRDILHRLAERCPCHVIVWPVPVQGEGAAPQIAAAVRGFNALPEDGPVARPD
                     LIIVARGGGSIEDLWAFNEEEVVRAVAASAIPTISAVGHETDTSLCDFAADVRAPTPT
                     AAAEMAVPVRADLVGQLRELGHRIQRCARRALDRAAEKLEQSLRRWPEREALLAPQRQ
                     RLDDLADRLPRALRTELSQARAALGQTAGALRPGLLKAAHGRARERLDALWRVAELAH
                     PSRPLRRGYARVEDRDGRTLVSAAAARRAERLRLIFGDGDVEARVGGAPGPVERPRKP
                     PYVKPPGEQPKLL"
     gene            2017..3291
                     /gene="purD"
                     /locus_tag="VF547_07745"
     CDS             2017..3291
                     /gene="purD"
                     /locus_tag="VF547_07745"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX8262751.1"
                     /translation="MNVLLLGSGGREHALAWKLAQSPRLGKLFAAPGNPGIAEHAQLV
                     GIDPADHRATVDFCLKNSVELIVIGPEAPLVDGLADNLRTMGFCVFGPNRIPAQLEGS
                     KGFTKDLCARHGIPTARYVRASDRLAAEAALADFPAPLVVKADGLAAGKGVVVCATRE
                     EADAALAAMFEGGFGAGGGAVVIEEFLEGEEASFFALTDGTTILPFGSAQDHKRVGEG
                     DTGPNTGGMGAYSPAPVLTPALEAVAMDEIVRPTVEALAAQGTPYSGILYAGLMLTAQ
                     GPRLIEYNARFGDPECQVLMMRLESDLLELLYATARGRLAEAEPPRLSADTALTVVLA
                     AEGYPGAPRTGGPIAGLEAAESADVRVFHAGTALRDGALVAAGGRVLSVTARGASLGE
                     ARDAAYAAVDRIDFPTGFCRRDIGWRELARRG"
     gene            3357..3791
                     /locus_tag="VF547_07750"
     CDS             3357..3791
                     /locus_tag="VF547_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262752.1"
                     /translation="MPTAVKLLLGLALALAAAWTWHGPLGRGEQRIARLESQARAAVA
                     QAELPGVAVRLDRRPLGRNAVLSGPANDLQREGLGSQKGVSDLVRDVPGIGAVRWADE
                     PARERRAPPLLAETMILAALAYLVGLGLGWLLWGRPRREGFA"
BASE COUNT          495 a         1382 c         1449 g          521 t
ORIGIN      
        1 tcgatcgtca gcgcccggtc caggctggcg aggccgcgtt cggcctcgcc ggcgctgtcg
       61 agggcctgga agaggttgaa ccagagcgcc gccgagcccg gctcggcggc ggcggcgcgc
      121 tcgatccact ggaccgcccg ccggccgtcg ccgcggttga gggcgatcat cccgagcgtg
      181 ttgagagcga cggaatggcc gggttcgatg tcgaccaggg cctcgagaag ctcggcggcg
      241 gcctcgtgcc ggccttgccc ccaggcctag gcggcgcggg cgtagaggtc cgccgggtcg
      301 gggcgcgccg ccgtcaacga ggccttcccg tccgggcgat cacccgcggc cttccgcctt
      361 caccgaaagc tccgccgacg aataggcctc ctgccgtgcc acgctccagt agcgcaggtc
      421 ctcgagcggg atgggctggc cggtggcggc gcagcggacg tggtcgccgg gcgtgaggat
      481 ccggaaggtc cccggcatgt agtggagtct cgcctcgcgg cccttgttcg acatcagcat
      541 cggcttcgcc tccttcgata cgccgtttcg ccgggctcaa cggctactca ggatgagcgg
      601 gaggcaattc gtcgccgccc aagatcctac agcaatttgg gctgttcgcc cgggggctta
      661 acataaggcg gcttgcgggg ccgctcaacc ggccccgggg cgccgccgac ccgagcctcg
      721 acgtcgccgt cgccgaagat caggcgcagg cgctccgctc ggcgcgccgc cgcggcggag
      781 acgagggtcc ggccgtcgcg gtcctcgacc cgggcatagc cgcgccggag cgggcggctg
      841 gggtgggcga gctcggccac ccgccacagc gcgtcgaggc gctcgcgggc tcggccgtgg
      901 gcggccttga ggaggccggg gcggagcgct ccggcggtct ggccgagagc cgcgcgggcc
      961 tggctgagct cggtgcggag cgcccgcgga aggcggtcgg cgaggtcgtc gaggcgctgg
     1021 cgctgcgggg cgagcagggc ctcgcgctcg ggccagcggc gaaggctctg ctcgagcttc
     1081 tcggcggccc ggtcgagggc gcggcgggcg cagcgctgta tgcgatgacc gagctcgcgc
     1141 agctggccga cgaggtcggc gcggaccggc acggccatct cggcggcggc ggtcggggtc
     1201 ggggcgcgga cgtcggcggc gaagtcgcac agcgaggtgt cggtctcgtg gccgacggcg
     1261 gagatggtgg ggattgcgga ggcggcgacg gctcggacca cctcctcctc gttgaacgcc
     1321 cacaggtcct cgatcgagcc gccgccccga gcgacgatga tgaggtccgg ccgggcgacc
     1381 gggccgtcct cgggcagggc gttgaagccg cgcaccgcgg ccgcgatctg gggcgccgcc
     1441 ccctctccct gcaccggcac cggccagacg atgacatggc acgggcagcg ctcggcgagg
     1501 cggtggagga tgtcgcggat gacggcgccg gtcggcgagg tgaccacccc gatcacccgc
     1561 ggctggaagg gcagccggcg cttgcgcgcg gcgtcgaaca ggccttccgc ggccagcgct
     1621 cggcggcgct tgtcgagcag ggccatcagc gcgccctggc cggccagctc gagtcgctcg
     1681 atgatgatct ggtagcgcga gcggccggga taggtggtga gcttgccggt cgccaccacc
     1741 tcgatgccgt cctcgggccg gaaggggagc gcggcggcgg tcgccttcca catcacgccg
     1801 tcgaggaccg ccttgtcgtc cttgaggcag aggtagcaat ggcccgaggc ggcgcgcttc
     1861 caccccgaaa tctcgccgcg caccctcacg aagccgaacg cgtcctcgac cgtgcgcttg
     1921 aggcgcatcg ccagctcgga gaccgaatag ggctccgaat tgtcgccggg gctcggggtt
     1981 gctacgaggc cgctctcggc cggagggaaa tcgtccatga atgtcctgct gctgggctcg
     2041 ggcggccgtg aacatgcgct cgcctggaag ctcgcgcaat cgcccaggct cggcaaattg
     2101 ttcgccgcgc ccggcaatcc ggggatcgcc gagcatgccc agctggtcgg gatcgacccc
     2161 gccgaccatc gggcgacggt cgatttctgc ctcaagaaca gcgtcgagct gatcgtgatc
     2221 gggccggagg cgccgctggt cgacgggctc gccgacaatt tgcgcacgat gggcttctgc
     2281 gtgttcgggc ccaaccggat accggcgcag ctggaaggct cgaagggctt caccaaggat
     2341 ctgtgcgcac ggcacggaat ccccaccgcc cgctacgtcc gggcctccga caggctggcc
     2401 gccgaggccg cgctcgcgga cttcccggcg ccgctcgtgg tcaaggcgga cgggctggcc
     2461 gcgggcaagg gggtggtcgt ctgcgccacc cgggaggagg ccgatgcggc gctcgcggcg
     2521 atgttcgagg gcggcttcgg agccggcggc ggagcggtcg tcatcgagga gttcctcgag
     2581 ggcgaggagg cgagcttctt cgccctgacc gacgggacga cgatccttcc gttcgggtcg
     2641 gcgcaggacc acaagcgggt gggcgagggc gacaccggcc ccaataccgg cggcatgggc
     2701 gcctacagcc ccgcccccgt gctcacgccg gcgctggagg cggtggcgat ggacgagatc
     2761 gtccgaccga cggtggaggc gctcgcggcc cagggcacgc cctattcggg catcctctac
     2821 gccgggctga tgctgaccgc ccagggccca aggctgatcg agtacaacgc ccgcttcggc
     2881 gatcccgagt gccaggtgct gatgatgcgc ctcgaatccg acctgctcga gctgctctac
     2941 gcgaccgccc gcggccgtct ggccgaggcc gagccgccgc gcctgtcggc ggacacggcg
     3001 ctgaccgtgg tcctcgccgc cgaaggctat cccggagcgc cccgcaccgg cggtccgatc
     3061 gccggcctcg aggcggcgga gtcggcggac gttcgcgtct tccacgccgg caccgcgctt
     3121 cgcgacgggg cgctggtcgc cgccggcggc cgggtgctct cggtcaccgc gcgcggcgcc
     3181 agcctcggcg aggcgcgcga cgccgcctat gcggcggtgg accggatcga cttcccgacc
     3241 ggcttctgcc ggcgcgacat cggctggcgc gagctcgccc gccgcggctg aggctggaca
     3301 gcaaagcggc ttcgctccta agcggtgccg tcgctgtcgc tcaggaggcg cccatcatgc
     3361 cgacggccgt caagctgctg ctcggactcg cgctggcgct cgccgccgcc tggacctggc
     3421 acggcccgct cggccgcggc gagcagcgga tcgcccggct cgaaagccag gcgcgcgccg
     3481 ccgtcgccca ggctgagctt cccggagtcg ccgtccggct cgaccgccgc ccgctcggcc
     3541 gcaacgcggt cctcagcggc ccggccaacg acctccagcg cgagggcctc ggcagccaga
     3601 agggcgtcag cgacctggtc cgcgacgttc ccggcatcgg cgccgtccgc tgggcggacg
     3661 agccggcgcg cgagcggcgc gcgccgccgc tgctcgccga gaccatgatc ctcgccgccc
     3721 tcgcctatct cgtcggcctc ggcctcggct ggctcctctg gggccggccg cggcgcgaag
     3781 gcttcgcctg accctggaag gaccgatccg gaatgatgaa cctcgcccaa gacaatctca
     3841 tcctcct
//
LOCUS       DASVQZ010000283         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_57851, whole genome shotgun sequence.
ACCESSION   DASVQZ010000283 DASVQZ010000000
VERSION     DASVQZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57851"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1033)
                     /locus_tag="VF547_07755"
     CDS             complement(254..1033)
                     /locus_tag="VF547_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8262753.1"
                     /translation="MAFEHIRLEQSPGAVATITIARPDRLNALSGHTVDELRTAVEEA
                     GSSGARCLLLTGEGRGFSSGADLAGSGGLPEDAGAALEKHFNPLIEAIFALPIPVVAA
                     VNGPAAGAGCSLALAADIVVAARSAYFLQAFVNIGLIPDAGATWLLPRLAGRARAMEM
                     MMLGERVSAETALEWGLISRVVEDEQLESEAAALATRLAQGPTVALGLIRKLAREAGH
                     RPLGETLAAERAAQRAAGETRDFRNAVMAFLQKRPARFEGA"
     gene            complement(1234..2118)
                     /locus_tag="VF547_07760"
     CDS             complement(1234..2118)
                     /locus_tag="VF547_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX8262754.1"
                     /translation="MADDPSGSGERSIWRGIRALLFGDDSEATLREEIEEAIESHEGE
                     APIVGDLSPIERQMLRNLLHFGESTVADIAVPRSDIIAVPATITFEGLVAAFAEAGHS
                     RLPVYEESLDSVVGMAHIKDVFTLQVLGADPPEDVTTLLRKPLYVPESMGVLDLLARM
                     RAERVHLAIVVDEFGGTEGLVTIEDVVEEIVGEIEDEHDEETPGMLIPVEDGIWDADA
                     RAELEEVAETIDARLGAVEEDVDTLGGLATVLAGHVPQAGEIVEHPSGWRLEVTESDS
                     RRVTRLRLHAPEEGALSE"
     gene            complement(2118..2627)
                     /gene="ybeY"
                     /locus_tag="VF547_07765"
     CDS             complement(2118..2627)
                     /gene="ybeY"
                     /locus_tag="VF547_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX8262755.1"
                     /translation="MIVVEADVSEEWDSSTDWAQLAKAAVRAAVAESDVPGLLESGVS
                     AEVSVRFTSDGELKGLNSAWRGKDKATNVLSFPMAGPEQLAALARTEGGEALLGDIVL
                     AQGICAAEAEEKGVGACAHAAHLVVHGMLHLLGFDHEGDEAEADRMEAAERRALAAMG
                     VADPYEMHR"
     gene            complement(2713..>3069)
                     /locus_tag="VF547_07770"
     CDS             complement(2713..>3069)
                     /locus_tag="VF547_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX8262756.1"
                     /translation="PAEQVERRIASGEIEIAPIAFMRGRTLNDAFIILDEAQNTTPAQ
                     MKMFLTRFGMRSRMVICGDPLQVDLPEPGKSGLADAVAKVEGIAKIATVRFGAGDVVR
                     HPLVGKIVEAYEGKAQ"
BASE COUNT          435 a         1093 c         1076 g          465 t
ORIGIN      
        1 gactatggcg tgacgccatt tcaggcgccc cttccgacgg cgcctcctgc gaggccccgg
       61 cgggtccgga cccagtacgc tcttcgacgg cgcgagctct gacgttaccg gatgacttcg
      121 cgccggccgt ccggcctcgg ggagcgagct ccctgggccg gtttccctgc cttgcgccgg
      181 gtgatcgggc cgactcgggc aggccttcca gctagaccag aaatgagaac aagtcaagaa
      241 cattgtgtac gggctaggcg ccttcgaagc gggcgggccg cttctggagg aaggccatca
      301 ccgcgttcct gaaatcccgc gtctccccgg ccgcgcgctg ggcggcgcgt tcggcggcca
      361 gcgtctcgcc caagggccgg tgcccggcct cccgcgccag ctttcggatc agcccgagcg
      421 ccaccgtcgg cccctgcgcc agccgagtcg ccagggccgc ggcctccgac tccagctgct
      481 cgtcctcgac cacccgcgaa atcagccccc attcgagcgc cgtctccgcc gacacgcgct
      541 cgccgagcat catcatctcc atcgcccggg cgcggccggc gaggcgcggc aggagccagg
      601 tcgcccccgc gtcggggatc agcccgatgt tcacgaaagc ctgcaggaaa taggcggagc
      661 gcgcggcgac gacgatgtcg gcggcgagcg ccagcgagca gccggcgccg gcggccggcc
      721 cgttcaccgc cgcgacgacc gggatcggca aagcgaagat cgcctcgatc aaagggttga
      781 aatgcttctc cagggccgca ccggcatcct cgggcaggcc gcccgaaccg gcgaggtcgg
      841 cgccgctcga gaagccccgg ccttcccccg tcagaagcag gcagcgggcg ccggagctcc
      901 ccgcttcctc gaccgctgtg cgcagctcgt cgaccgtgtg gccggaaagg gcgttgagac
      961 ggtcgggccg cgcgatggtg atcgtggcga ccgcgccggg actctgctcg agccggatat
     1021 gttcgaaagc catggccgct tacagcatca aggagacggc cgcgcaactt gcctccccat
     1081 gggcaggcat ggggaggggg accaccgaag gcggtggagg ggtgacaagc cggggaaggc
     1141 ggaggttcag gggagatccg ccgcggtcga gtcggatcac gcctccgcca ggctccgcct
     1201 ggtcccactc cccatccggc gatggggagg cagttactcg ctcagcgccc cctcttccgg
     1261 cgcgtgcagc ctgagccgcg tgacgcggcg cgagtcgctc tccgtcacct cgagccgcca
     1321 gcccgacgga tgctcgacga tctcgcccgc ctgcgggacg tggccggcga ggacggtggc
     1381 gagtccgccg agcgtgtcga catcctcctc gaccgcgccg aggcgggcgt cgatcgtctc
     1441 ggcgacctcc tcgagctcgg cgcgcgcgtc ggcgtcccag atgccgtcct cgaccgggat
     1501 cagcattccg ggagtctcct cgtcatgctc gtcctcgatc tcgccgacga tctcctcgac
     1561 cacgtcctcg atggtgacta gcccctcggt gccgccgaac tcgtcgacga cgatggcgag
     1621 atggactcgc tcggcgcgca tccgggcgag gaggtcgagc acgcccatgg attcgggcac
     1681 gtagaggggc ttgcggagca gggtggtgac gtcctcgggc ggatcggcgc cgagcacctg
     1741 cagggtgaag acgtccttga tgtgggccat gccgacgacg ctgtcgaggc tctcctcata
     1801 gacgggcagg cggctgtggc ccgcctcggc gaacgcggcg accaggccct cgaaggtgat
     1861 ggttgcgggg accgcgatga tgtcgcttcg cggcaccgcg atgtcggcga cggtgctctc
     1921 gccgaaatgc agcaggttgc ggagcatctg ccgctcgatc ggcgagaggt cgccgacgat
     1981 cggcgcttcg ccctcgtggc tctcgatcgc ctcctcgatc tcctcgcgaa gcgtcgcctc
     2041 gctgtcgtcg ccgaacagca gggccctgat gccccgccag atgctgcgct cgccggagcc
     2101 gctcggatcg tcggccatca gcggtgcatc tcgtagggat cggcgacgcc catggcggcg
     2161 agggcgcgcc gctcggccgc ctccatcctg tcggcctcgg cctcgtcccc ctcatggtcg
     2221 aagccgagca gatggagcat gccgtggacg acgagatggg cggcgtgggc gcaggcgccg
     2281 acgcccttct cctcggcctc cgcggcgcag atgccctggg cgaggacgat gtcgccgagc
     2341 agcgcctcgc cgccctcggt gcgggcgagc gccgccagct gctcgggccc ggccatcgga
     2401 aaggacagga cgttggtcgc cttgtccttg ccgcgccagg ccgagttcag cccctttagc
     2461 tcgccgtcgg aggtgaagcg gaccgagact tcggcggaga cgccgctttc gagcaggccc
     2521 ggaacgtcgc tctcggccac ggcggcgcga acggcggcct tggccagctg cgcccagtcg
     2581 gtgctactgt cccactcctc cgagacgtcg gcttcgacga cgatcatgac gcgtcgttcc
     2641 ggcggcaacc ggcgctgcgg cagatccctc tggggccccg gccttcgctg ggacgacgtc
     2701 tcgagacggg gatcactgcg ccttgccctc ataggcctcg acgatcttgc cgaccagcgg
     2761 gtggcggacc acgtcgccgg cgccgaagcg gaccgtcgcg atcttggcga tgccctctac
     2821 cttggcgacc gcgtcggcga gtccggactt gccgggctcg ggcagatcga cctggagcgg
     2881 atcgccgcag atcaccatcc ggctgcgcat cccgaagcgg gtgaggaaca tcttcatctg
     2941 cgccggggtg gtgttctgag cctcgtcgag gatgatgaag gcgtcgttga gcgtgcggcc
     3001 gcgcatgaag gcgatcggag cgatctcgat ctccccgctg gcgatgcgcc gctcgacctg
     3061 ctcggccgg
//
LOCUS       DASVQZ010000284         5233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_925268, whole genome shotgun sequence.
ACCESSION   DASVQZ010000284 DASVQZ010000000
VERSION     DASVQZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925268"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF547_07775"
     CDS             <1..415
                     /locus_tag="VF547_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8262757.1"
                     /translation="KIATDLGVSEEDVISMNRRMAMGGDTSLNVPLREEGDGQWQDWL
                     VDTDPLQDERVAEAQESQVRHELLIEAMKALNDREKHILTERRLTDEPKTLEELSQVY
                     DVSRERIRQIEVRAFEKLQKALMGLAGERRLLPAS"
     gene            complement(501..1586)
                     /locus_tag="VF547_07780"
     CDS             complement(501..1586)
                     /locus_tag="VF547_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8262758.1"
                     /translation="MKWLLPIAALFAVQPVSAQAPAAPAEAPIVLGTRFALPSAVLGE
                     AREINVRLPASYALAKADRRYPVLYLLDGGVEQDFPHIAGLAQHGEISWTFDEFIVVG
                     IATKKRIWELTIPTRDERYTTYFRANGEPVEFASGGGSETFRRHIAEEVIPFVEGKYR
                     TSGRRTFIGESLAGLFVVDTLLRKPELFHDYVAISPSMWWNREELGDRARELLDGRDR
                     SGRRLYLTMGSEGGTMQRGMDKLLAALRSPAAGALQWTYVDRRASEHHGSIYHAAALD
                     ALRTLYPKPWRPGSPLPWLFIGEMPRLSPEAETDKKIECTAERARPVTFAEVNADPRK
                     WEALCVLPPLGQGPQPRERSRNWGKAP"
     gene            1695..2399
                     /gene="mtgA"
                     /locus_tag="VF547_07785"
                     /pseudo
     CDS             1695..2399
                     /gene="mtgA"
                     /locus_tag="VF547_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(2604..2921)
                     /locus_tag="VF547_07790"
     CDS             complement(2604..2921)
                     /locus_tag="VF547_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX8262759.1"
                     /translation="MPDLDEIMKMFQNTQAGLMKAQEDLDKIEVEGQSGAGMVKIRAT
                     AKGRILSVEIDESLLVPSEKQMVEDLVAAAINDARLKADQAAQVEMQKMTAGLQLPPG
                     FNF"
     gene            complement(2921..4576)
                     /locus_tag="VF547_07795"
     CDS             complement(2921..4576)
                     /locus_tag="VF547_07795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX8262760.1"
                     /translation="MSDSPDSFGLGLDEPPQPAKAQPYRVLARKYRPQSFAELIGQDA
                     MVKTLGNAIERDRLAHAFLLTGVRGVGKTSTARLIAKALNCVGADGQGGPTIEPCGVC
                     EPCRAIAEGRHIDVVEMDAASHTGVDDVREIIEAVRYAAVSARYKVYIVDEVHMLSKN
                     AFNALLKTLEEPPAHVKFLFATTEVNKVPVTVLSRCQRFDLRRIPAEKLAAHFGEVAE
                     KEGVEVEPEALALIARAAEGSARDGLSILDQAIAHGPGGVTAEQVRTMLGLSDRGAIR
                     RLLGVILSGDAAAALAALRDQYDLGVEPSAVLRGLLESVHGITRAKVGGADDPAQSAE
                     EREAYAEWGSSLSYAAVHRLWQLLLKGLAEVHGAPMPLEAAEMALLRVIHASELPDPG
                     ALLEKLASGEGAAASAPAAAAGPSGQAPMLEAPPSFEAFADLIETRGKAILAHQLREN
                     YRLVEYGPPSLLLRQTGNVREVRDVEAFLRTLREATDAIFGQKWQVALSDGPAQPTLR
                     EQEQAAEAELKRKVLESPLVKAAFEAFPEAELSGFTLDEQRSA"
     gene            complement(4614..>5233)
                     /locus_tag="VF547_07800"
     CDS             complement(4614..>5233)
                     /locus_tag="VF547_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262761.1"
                     /translation="TTVVQGSLGVLESPARTRERVIRHRIVPIAAGCDNSLLNASFAD
                     RSEWPGPPPPPYLWDMLAELAAKGRCFRSDWSHDGRADIYLVQEYGFPSAAPKPARFD
                     LRLVKFDPPQRWAVLRRQGGRLIPIVRRTRLVVRKLAVPLTFSPPEEFASFRPGGWGV
                     GSQEIRGREQFQGLMRPWLRNDLRLAGLGRNRPADIVVEGRRGRD"
BASE COUNT          794 a         1792 c         1872 g          775 t
ORIGIN      
        1 caagatcgcg accgatctcg gcgtgtccga ggaggacgtc atctcgatga accgccggat
       61 ggcgatgggc ggcgacacgt cgctcaacgt gcccctgcgc gaggagggcg acggccagtg
      121 gcaggactgg ctggtcgaca ccgacccgct ccaggacgag cgggtcgccg aggcgcagga
      181 gagccaggtc cgccacgagc tgctgatcga ggcgatgaag gccctgaacg accgcgagaa
      241 gcacatcctc accgagcgcc gcctcaccga cgagcccaag acgctcgagg agctctcgca
      301 ggtctacgac gtcagccgcg agcgcatccg gcagatcgag gtaagggcgt tcgagaagct
      361 gcagaaggcg ctgatgggcc tcgccggcga gcggcggctg cttccggcga gctagcgtca
      421 ttgcgagcga agcgaagcaa tccagccgcg gcgttggacg cgtgtagatt gcttcgtcgc
      481 ttcgctcctc gcaatgacgg ctagggcgcc ttcccccagt tcctggaccg ctcgcgcggc
      541 tgcgggccct ggccgagcgg gggcaggacg cacagcgcct cccatttgcg cggatcggcg
      601 ttgacctccg cgaaggtcac cggccgcgcg cgctcggccg tgcactcgat cttcttgtcc
      661 gtctccgctt ccgggctcag cctgggcatc tcgccgatga acagccaggg cagcggcgag
      721 ccgggccgcc agggcttggg gtagagcgtg cgaagcgcgt cgagcgccgc cgcgtggtag
      781 atgctgccgt gatgctcgct ggcgcgccgg tcgacgtagg tccattgcag cgctcccgcc
      841 gccggcgacc gaagcgcggc gagcagcttg tccatgccgc gctgcatcgt cccgccctcg
      901 ctgcccatcg tcaggtagag ccgccgtccc gaacgatcgc ggccgtcgag cagctcgcgg
      961 gcgcggtcgc ccagctcctc gcggttccac cacatgctgg ggctgatcgc gacataatcg
     1021 tggaacagct ccggctttct caggagggtg tcgacgacga acaggcccgc gagcgactcg
     1081 ccgatgaacg tgcgccggcc gctcgtccgg tacttgcctt cgacgaaggg gatgacctcc
     1141 tcggcgatgt ggcgtcggaa cgtctccgaa ccgccgcccg aggcgaactc gaccggctcg
     1201 ccattggccc tgaagtaggt cgtgtagcgc tcgtcccggg tcgggatggt gagctcccag
     1261 atgcgcttct tcgtggcgat tccgacgacg atgaactcgt cgaacgtcca gctgatctcg
     1321 ccatgctggg cgaggccggc gatgtgcggg aaatcctgct cgacgccgcc gtcgagcaaa
     1381 tagagcaccg gatagcggcg atcggccttg gccagcgcat agctggccgg gagccggacg
     1441 ttgatctcgc gcgcctcgcc cagcaccgcc gagggcaagg cgaagcgggt gccgaggacg
     1501 atcggggcct cggccggcgc cgccggcgcc tgggccgaca ccggctggac ggcaaacagg
     1561 gcggcgatgg gaagaagcca tttcattcgg agcactcctg gacgcggcgg tccgagcgct
     1621 atccgccccg cccggccgtg gacaagcggc aagttcagca tgacgccggc ggccggggcc
     1681 gctaacggac cgcgatggca cgtgtgcggg caaggaaagg cggctggctc ggccgcgcga
     1741 tcggctggat cgtcaaggga atcttcctct tcatcctgct cagcctgctg tgggtgctgc
     1801 tctaccgctt cgtgaacccg ccctatacgc tgaccatgct cggcgaccgg ctcggcgggc
     1861 gcaacgtggc tcgcgactgg atgggcctcg ggtcgatcga ccgcgacatg gtccgagccg
     1921 cgatcgccgc cgaggacagc atgttctgca cccactgggg cttcgaccag gatgcgatcg
     1981 ccgcggcgat gcggcgcaac gcccggggcg gatcggtcat ccggggcggc tcgacgatca
     2041 gccagcagac cgccaagaac gtcttcctgt ggcagggcgg cggctatttc cgcaaagggc
     2101 tcgaagcctg gttcaccctg ctgatcgaga atctgtgggg caagcggcgg atcatggagg
     2161 tctatctgaa cgtcgccgag accggcatcg gcacctacgg cgccaatgcc ggcgcccagc
     2221 gctacttcaa ccacgacgca agcgcgatga gccgcaccga ggcggcgagg atcgccgccg
     2281 tcctgcccct gcccaagaag cgcggagcga cggcgcccaa gggcttcacc cgccgctacg
     2341 gcaatgcgat cgccgcgcgg atcggcgtgg tcggccggga ggggctggat cgatgcgtct
     2401 acgagggcaa ggcggcggcg ccggaccgcg aacccgcacc gccggcgagg cggaaggcgg
     2461 cgccccggac gaaggcggcg gcgccgccgc cgccggccgc gcttcccggc gaggaatatg
     2521 agcagccgca gccggccccg gcggccgagc ccgcgccgac gcccgagccc gagccggcgc
     2581 cggaggcgaa cggccagtag cggtcagaag ttgaagccgg gcgggagctg gaggccggcg
     2641 gtcatcttct gcatctcgac ctgggcggcc tgatcggcct tcagccgcgc gtcgttgatc
     2701 gcggcggcga cgaggtcctc gaccatctgc ttctcggacg gcaccagcag gctctcgtcg
     2761 atctcgaccg agaggatccg gcccttcgcg gtggcgcgga tcttgaccat cccggcgccc
     2821 gactggccct cgacctcgat cttgtcgaga tcctcctgcg ccttcatgag gcccgcctgg
     2881 gtgttctgga acatcttcat gatttcgtcg agatccggca tcacgcgctc cgttgttcgt
     2941 ccagggtgaa gccggacagc tcggcttcgg gaaaggcctc gaaggccgcc ttgaccaaag
     3001 gcgattccag caccttgcgc ttcagctcag cctcggccgc ctgctcctgc tcgcgcaggg
     3061 tcggctgcgc tggaccgtcc gacagagcga cctgccattt ctggccgaag atggcgtccg
     3121 tggcctcgcg cagggtgcgc aggaaggcct cgacgtcgcg gacctcgcgg acgttgccgg
     3181 tctgccggag gaggaggctg ggcgggccgt actcgacgag ccggtagttc tcgcgcagct
     3241 ggtgggcaag gatcgccttg cccctcgtct cgatgaggtc ggcgaaggcc tcgaacgaag
     3301 gcggggcctc gagcatcggc gcctggcccg aaggccccgc cgcggcggcg ggagcggaag
     3361 cggcggcccc ctccccgctc gccagcttct cgagcagggc gcccggatcc ggaagctcgg
     3421 aggcatggat gacccggagc agcgccattt ccgccgcttc gagcggcatc ggcgcgccgt
     3481 ggacctcggc gaggcccttc aggagcagct gccagaggcg atggacggcg gcataggaga
     3541 ggctgctccc ccattcggca taggcttccc gctcctcggc cgactgggcg ggatcgtccg
     3601 cgcccccgac cttggcccgg gtgatgccgt ggacgctctc gagcaggccg cgaagcacgg
     3661 cgctcggctc cacgccgaga tcatattggt cgcgcagggc ggcgagcgcg gcggccgcgt
     3721 cgccgctcaa tatcacgccc agcagccggc ggatcgcgcc ccggtcggac aggcccagca
     3781 tcgtccgcac ctgctcggcg gtgacgccgc ccgggccgtg ggcgatggcc tggtcgagga
     3841 tcgagagtcc gtcgcgggcg gagccctcgg cggctcgggc gatcagggcg agcgcctcag
     3901 gctcgacctc gacgccttcc ttctccgcca cctcgccgaa atgggcggcg agcttttcgg
     3961 ccggaatgcg cctgaggtcg aagcgctggc atcgcgagag gacggtgacc gggaccttgt
     4021 tgacctcggt cgtcgcgaac aggaacttaa cgtgtgcagg gggttcctcg agggtcttga
     4081 gaagcgcgtt gaacgcattc ttcgacagca tgtggacttc gtcgacgata tagaccttgt
     4141 accgcgccga gacggcggcg tagcgcaccg cctcgatgat ctcgcgcacg tcgtcgacgc
     4201 cggtatggct tgccgcgtcc atctcgacga cgtcgatgtg acggccttcc gcgatcgccc
     4261 ggcacggctc gcagaccccg cagggctcga tggtcgggcc gccctgcccg tccgcgccga
     4321 cgcagttcag agccttggcg atcagccggg cggtcgaggt cttgccgacc ccgcgcacgc
     4381 cggtgaggag gaaggcgtgg gcgagcctgt cgcgctcgat cgcgttgccg agcgtcttca
     4441 ccatcgcatc ctggccgatc agctcggcga agctctgcgg ccggtacttg cgggcgagga
     4501 cccggtaggg ctgcgccttc gccggctggg gcggctcgtc caggcctagg ccgaaggaat
     4561 cgggggaatc ggacatgggc tgaaactagc ggttaaacct ggccccgtcg aaatcaatcg
     4621 cgtccgcgcc tgccctcgac gacgatgtcg gccggccggt tccggcccag tcccgcgagg
     4681 cgcaggtcgt tcctcagcca gggcctcatc aggccctgga actgctcgcg gccgcggatt
     4741 tcctggctgc cgacgcccca cccgcccggc cggaagctgg cgaactcctc gggcggcgag
     4801 aaggtgagcg ggacggcgag cttcctaacc accagccggg tgcggcgaac gatcgggatc
     4861 agccggccgc cctgccgccg caggaccgcc cagcgctggg gcgggtcgaa cttgaccagt
     4921 cggagatcga agcgcgcagg tttcggtgcg gcgctcggga agccatattc ctggacgagg
     4981 tatatgtcgg cgcgcccgtc gtgcgaccag tcggaccgga agcatcgccc cttcgcggcg
     5041 agctcggcga gcatgtccca caaatagggc ggcggcggcg ggcccggcca ctcgctgcgg
     5101 tcggcgaagg aagcgttcag caggctgttg tcgcagcccg cggcgatcgg cacgatgcgg
     5161 tggcggatca cgcgctcgcg ggtgcgggcg gggctctcga gcacgccgag gctgccctgg
     5221 acgaccgtcg tcg
//
LOCUS       DASVQZ010000285        13987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_925324, whole genome shotgun sequence.
ACCESSION   DASVQZ010000285 DASVQZ010000000
VERSION     DASVQZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13987
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925324"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VF547_07805"
     CDS             <1..1389
                     /locus_tag="VF547_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8262762.1"
                     /translation="ASAMTRAHPEFAGYSGSIDFLLPADPERPVFAGFPIDTLPLLGE
                     AIRDRIRGRYVLIGGNIKDQDDYETPMSRSTGRWMKGVEVHAHMLAQQLDGRMRPPLP
                     PFLLWVNAIALVVAGGVSSLLEWRGWRLAVVLAAQLGVIAVSPFLLQGWGLDTYGVPA
                     FGPALGWAVAFAAVGTAARAVGSEQRRFAQATLGKYLPADVAAEILRDPDRLTLTGEK
                     RQIYALFSDLEGFTKLSHATSPEQLSSLLNRYLDVLSEVVLRHGGTIDKFVGDAVVAF
                     WGAPIARDDDADRAVLAAVAMYEAGEEFRRTAGPGLPAIGCTRVGLHRGEAVVGNFGG
                     EGRIQYTALGDGMNTASRLESANKLLHSTILVSDEARRGTSLDIFRPLGRIVLSGRAT
                     PVEVWEPVPHMPADERQRLVRLWQAFEAGDPHALETLRAIAAEREDAALADLVYRLGE
                     TGPGGHFVLGSK"
     gene            1370..2080
                     /locus_tag="VF547_07810"
     CDS             1370..2080
                     /locus_tag="VF547_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262763.1"
                     /translation="MSWGPNSVLARPLIALGLLATATAAMADILVVRSSGPSARSYPA
                     GRRLPESSRLSLKAGDQLIVLDGRGTRVIRGPGSFAAGAPAVGRVASAAPVQRRARIG
                     AVRGIETGELRPPSIWHVDVARSSNVCVADPAKVTLWRARAAEAATLSIAARNGATRQ
                     LGWQPGSSTLAWPSDLPVAEGAEYRLSGTGAAAPTTIRFRTMPAKPAGLEDMAQALIR
                     NQCDAQLDLFIDTVKLPG"
     gene            complement(2213..4387)
                     /locus_tag="VF547_07815"
     CDS             complement(2213..4387)
                     /locus_tag="VF547_07815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX8262764.1"
                     /translation="MKHILLTTAAALGLGACASTAADEGPTTAAAAAPAQAPAEPAVV
                     VPDNVLLADWTGPYDGVPPWDQVKPELFPEAIQFGIDEQRREVLAIADSPDAPTFANT
                     VEALEKAGQRLDRVLSMFAVMTDNMTTPAYQALEKEWSPKLSAASDEIILNPKLFQRI
                     KTVYEMRENANLDAKQQRLVTRLYDSFVRRGANLSPEEKQQLTAYNSQLAQKFAVFGE
                     KVLADESSYITATDAELKGVPADVKAAAASAAQERKLPAGTFAIVNTRSAVDPILTFA
                     DNRALREKVWRAFVNRGDNGGANDTNAVIAEIVKLRADRAKLLGFSSHAEFRMQDTMA
                     KSPAKALDLMNRVWPAAVARVKEEVADMQALARKTDRSLTIEPWDYRYYQEKVRKQRY
                     NLTQEEVKPYFELNNIIEASYWAAGELYGLDFKEITGTVPTWHPDIRVYSVTDRGSGK
                     QIGVFYRDDYARTGKRSGAWATTYRSRAGLLGDDVVLGSNNNNFVKPAPGQPVLISLD
                     DAETLFHEFGHAIHYFLSNVYYPSFGGTPRDFVEYPSQVNENWLLTQPVLSRFAKHYK
                     TGQPMPAALIAKIEKASTFNQGFATAEYLSSAIVDMMLHMDPNGVVDPDRFEREALAK
                     IGMPRELAMRHRLPQFNHLFSSDAYSAGYYSYLWSETMDADTWAAFEEAGNPFDKATA
                     DRFRQTLLSTGNETDRAEAYRLFRGRDPDVKALMRKRGFPTN"
     gene            4619..5149
                     /locus_tag="VF547_07820"
     CDS             4619..5149
                     /locus_tag="VF547_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8262765.1"
                     /translation="MLNILIVEDDPLLAETVKSLVELNPRFQVTALANDLASAVAAVE
                     DRRPDLALVDLQLANGSSGYAVAARLNDYGIACLFSTAKAPGFAVRDLAIGCLQKPYR
                     EEDLVRALTEAEDILKGRPKLVLRPSLPEPLEIYGEEDKAGEPAFALAIGGRTSWLAK
                     LKKLVRRPSSFRSAAA"
     gene            5459..6802
                     /locus_tag="VF547_07825"
     CDS             5459..6802
                     /locus_tag="VF547_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX8262766.1"
                     /translation="MGASRNLASGALAASLLLALAVFLSPAGAQQAAAGHYVGVATCS
                     GSTCHGRSEATGAIVRQDELRLWQDPSSPAGAHSRAWRVLTEPRARAIAARLGIGEAA
                     SAPMCLGCHATPASGARGPRFQISDGVGCESCHGAASNWLASHYAVGGTHSANVSRGL
                     VPLDDPRARAGKCLDCHFGSAGSGQFADHRMMAAGHPRISFELDLFTTLQQHHDEDGD
                     YVRRKGRSNAVANWAVGQATALERSLALFATPGRGTNGMFPEFYFFDCHSCHRRISDD
                     PRFEPSKAANPARPIPSGTPPYNDENMIMLAAAARVAAPQAAPRFERDSRRFHAAMTR
                     DRASALAAAAALKESARAVAADLARGVSRDQVFAIIDSIAGDAISPRFTDYTGSVQAV
                     MAVDTLFSSLGAGAPESIRADINGAYRAVRDPNAYDPREFRASLGRAAAAIRKLK"
     gene            6799..8736
                     /locus_tag="VF547_07830"
     CDS             6799..8736
                     /locus_tag="VF547_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX8262767.1"
                     /translation="MRRGRALACIAAMVVSSCGGGGGGSPPPPPPPPPAPAPPAPPAG
                     VYPVPAQLSLSQAEVQQIVARGVAEAQARSLPATIAVVDRVGNVLAVFAMNGANPRLQ
                     IPRGPNGSTHDLQGVDLPAAAAGAIAKAITGAYLSSGGNAFSTRTASMIVQEHFPPSA
                     AAKGLESGPLFGVQFSQLPCSDLAARFNGNAGAGGFIGPKRSPLGLAADPGGFPLYKD
                     GVLVGGVGVMADGVYGFDPEVQDVDKDREEAIALAAATGLAAPEEIRAERISVDGTLL
                     RFSDAAPSDLLSNPSAAPGFAAINGSAGALASVRGYYDSTMGVLAGRAYGTEASGVRP
                     ATAAEFPNPDAFVLSNGGGSNRFAVRAGTDGGLTAAEVQALLEEAFKVMTAARAQIRR
                     PLDSRAQVSISVVDSSGAVLGLVRSPDAPVFGIDVSLQKARTAAFFSSANAAATIEAS
                     GDSDVRQFVAAARTFFGNPAAFTGSFAFTTRAIGNISRPYFPDGELGRPPGPFSRPIA
                     EFNPFSTGLQSALVEADVKAHVGFVLGNPADTPAQCTHTPRLANGMQIFPGSVPIYRG
                     ATLVGAIGVSGDGIDQDDMISFLGVHNAGLRAGGFGNADPARRSDQIVVPVGSGVRLR
                     YVGCPFAPFLGSNEQNVCQGK"
     gene            8733..10649
                     /locus_tag="VF547_07835"
     CDS             8733..10649
                     /locus_tag="VF547_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262768.1"
                     /translation="MSFGLVLGLLAGASGIALQSPSPQPRPDDEPGVQDRPTQEEPDP
                     LPNIPTEEGRRRPGIPQSELDDPVSQDNPGAIRAPPPEAFPTDQIPVPDRWRLIESLG
                     VVKERWFDPYNQNSYKGDRPLCLPTPEEQARRRAEGRPRCRTPRFLGLDEDDWFLVVN
                     AVSDTVVEPRTFPIPVGVQTTAGPGSLDVFGKNDSLALSQTFIAGAALVKGSTAYMPP
                     HVEYRLTLALNVNYVDVAERRVLFVEPSKGTSRLDYALGVQEAFVDYHLRNVSDRYDF
                     DSVRVGIQPFQLDFRGFLFNDQQLGVRLFGNRRSNRFQYNLMAIWRLEKDTNSGLNNV
                     LQSPRKDWILHANLYRQDFPFVGLTSQASVTVNLNRERGDIEVDDNGFPVRPALIGNL
                     RARNYEVAYLGYSADGRIGRINLSASAYGAFGWDRNNIFTGETGKIRSFFLAAEPSYD
                     LNWMRFRGAFLFASGDGEPFDDREGGFDAIFENPVFAGADTSYWIRQSVPFAGGGRAV
                     SINGRNGILNSLRSSKEQGQSNFNNPGTILIGAGADFDLTPELRVSGNVNHLWFHETA
                     VLQALRNEGSIPRDIGWDVSAAAIWRPKMTQNIVLRASAAVLQPGAGFRDLFFNSDRD
                     KRYYSVLLNAIATY"
     gene            10757..>13987
                     /locus_tag="VF547_07840"
     CDS             10757..>13987
                     /locus_tag="VF547_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX8262769.1"
                     /translation="MTMFLGLLATPLLASEGGKPVARDHALVRAGPAPRTQEWDSAEA
                     KSAGCVSCHVGSDQKTMHASPAVVLGCVDCHGGNASVQGDASLPHDGPAYVAVRDKAH
                     VLPRYPKAWHWPSSANPKRSYALLNAESPEYVRFVNPSDYRVARDSCGACHIETIEAA
                     ERSLMASGAMLWGGAAYNNGILPYKNYLFGEAYTRHGEPARIVSPPSPEAVTPEQKAR
                     GVLAELYPLPTWHVVPPGDVFRVFERGGRTVGTQFPEIGLPSPSGSIQRLEEAGRPDI
                     RQSNRGPGTGLRVAIPVLNIHKTRLNDPFMWFMGTNDQPGDYRHSGCAGCHVVYANDR
                     EPRHSLTYAQFGRDGQTATADPTIAGKTEHPGSPTAAAAPADAAHGSLKEKGHPIRHA
                     FTRAIPTAQCMTCHMHQPNIFLNSFLGYTMWDYEADADLMWPGPENRLPRKDGEERSL
                     GRQRYPDAAQALEILDRNPEAAATRGLWGDVDFLREVNDRVNPLARHTQFADYHGHGW
                     NFRAVYKRDREGNLLDSDGKVVAPDDPEKFGRTGSPQFVPPGEQKGKAVHMMDIHAEK
                     GMQCADCHFAQDSHGNGFLYGEVANAVEIGCKDCHGTAGRYPNLLTSGPAAPPRGTNL
                     ALLRNQDGRRRFEWMAGEDGRRVLVQRSIVDPALEWEVSLVKDSLDPRFPGFNLKAAR
                     AKLMSREGSESGRYVFGSGIASGNLAHKDDEMACFTCHLSWTTSCAGCHLPIEANWKT
                     KSNRYDSSETRNFATYNPQVARDDMFQLGRHQTTKGNIVAPVRSSSALVLSSTNINRE
                     RIYVQQPPISAAGFSSQAFAPHFPHTVRKTETKTCTDCHLSQQDDNNAVMAQLLLQGT
                     NFVNFVGLHAYVGLDGGFQATRVTEWDEPQAVIGSYLQRFAYPDFWRQHVDRNKRELT
                     NWTRGGPGSREQFANIFQGTGGAVGCLQQRGEYMFVAEARGGFRVYDIASLGNKGVSE
                     RIVSAPFSPLGQDMKVASRNATCLALPTNQPISVGRNDKVVKNMPENQEQAFHPIYRY
                     AIITDSEEGLILVDVETFADGEPRNNFIKRAVTWNEGGVLNGARHIT"
BASE COUNT         2123 a         5050 c         4658 g         2156 t
ORIGIN      
        1 gccagcgcga tgacccgcgc ccatcccgaa ttcgccggct attccggcag catcgacttc
       61 ctcttgcccg cggatcccga acggcccgtc ttcgccggct tcccgatcga caccttgccg
      121 ctgctcggcg aggcgatccg cgaccggatc aggggccgct acgtgctgat cggcggcaac
      181 atcaaggacc aggacgatta cgagacgccg atgagccgga gcaccggccg ctggatgaag
      241 ggggtcgagg tccacgccca catgctcgcc cagcagctcg acggccgcat gcgcccgccc
      301 ttgccgccct tcctgctgtg ggtgaacgcg atcgccctgg tcgtcgcggg cggggtcagc
      361 agcctgctcg aatggcgcgg gtggcgcctc gccgtggtgc tggcggcgca gctgggggtc
      421 atcgccgtct cgcccttcct cctccagggc tgggggctcg acacctacgg cgtccccgcc
      481 ttcggcccgg ccctgggctg ggcggtcgcc ttcgccgccg tcggcaccgc cgcccgcgcc
      541 gtcggctcgg agcagcgccg cttcgcccag gcgacgctcg gcaaatatct gcccgccgac
      601 gtcgcggccg agatcctcag ggatccggac cggctgaccc tcaccggcga gaagaggcag
      661 atctacgccc tcttctccga tctggagggc ttcaccaagc tcagccacgc gaccagcccg
      721 gagcagcttt ccagcctgct caaccgctat ctcgacgtcc tgagcgaggt cgtcctgcgc
      781 cacggcggga cgatcgacaa gttcgtcggc gacgccgtcg tcgccttctg gggagcgccc
      841 atcgcgcgcg acgacgacgc cgatcgcgcc gtcctcgccg ccgtcgccat gtacgaggcc
      901 ggcgaggagt tccgccgcac cgccgggccg gggctgcccg cgatcggctg cacccgggtc
      961 ggcctccacc gcggcgaggc cgtggtcggc aatttcggcg gcgagggccg gatccagtac
     1021 acggcgctcg gcgacggcat gaacacggcg tcgaggctgg aatccgccaa caagctgctc
     1081 cactcgacga tcctggtcag cgacgaggcg aggcgcggga cgagcctcga catcttccgc
     1141 ccgctcggcc ggatcgtcct cagcggccgc gcgaccccgg tcgaggtgtg ggagcccgtc
     1201 ccgcacatgc cggcggacga gcggcagcgg ctcgtgcgat tgtggcaggc cttcgaagcc
     1261 ggcgacccgc acgcgctgga gacgctgagg gcgatcgccg ccgagcgcga ggatgcggcg
     1321 ctcgccgatt tggtgtatcg cctgggcgag acaggtccag gaggtcattt tgtcttgggg
     1381 tccaaatagc gttctcgccc ggccgctgat cgcgctcggc cttctcgcca ccgccaccgc
     1441 ggccatggcc gacatcctcg tcgtgcgctc gtccggcccc tcggcgagaa gctatccggc
     1501 cggcaggcgc ctcccggaga gcagccggct cagcctcaag gccggcgatc agctgatcgt
     1561 gctggacggc cgcggcaccc gagtcatccg cggcccgggg agcttcgcgg cgggcgctcc
     1621 cgccgtcggc cgggtcgcct cggcggcgcc cgtccagcgg cgggcccgga tcggcgcggt
     1681 gcgcgggatc gagaccggcg agctgcgtcc gccgagcatc tggcacgtcg acgtcgctcg
     1741 aagctccaac gtctgcgtcg ccgatcccgc gaaggtgacc ttgtggcggg cgcgggccgc
     1801 ggaagcggcg accctgagca tcgccgcccg caacggggcg acccggcagc tgggctggca
     1861 gccgggctcg agcaccctgg cctggccgtc cgacctgccc gtcgcggagg gcgcggaata
     1921 ccggctcagc ggcaccggcg cggcggcgcc gacgacgatc cgcttccgca ccatgccggc
     1981 caagccggcc gggctcgagg acatggccca ggccctgatc cgcaaccagt gcgacgccca
     2041 gctcgacctg ttcatcgaca ccgtgaagct gccgggctga atatcctccc caatctacgt
     2101 ttggggaggg ggaccccccg aaggggggtg gaggggtagc tggagcagac ctgcgccaat
     2161 acccctccac cccgctgtgc ggggtccccc tccccatctc cgatggggag gatcaattcg
     2221 tcgggaaccc cctcttcctc atcagcgcct tgacgtccgg gtcgcgcccg cggaacagcc
     2281 ggtaggcctc ggcccggtcg gtctcgttgc cggtcgacag cagcgtctgg cggaagcggt
     2341 cggcggtcgc cttgtcgaac ggattgcccg cctcctcgaa cgccgcccag gtgtcggcgt
     2401 ccatcgtctc cgaccagaga taggaataat agccggccga ataggcgtcg gaggagaaga
     2461 ggtggttgaa ctgcggcagc cggtgccgca tcgccagctc cctgggcatg ccgatcttcg
     2521 ccagcgcctc gcgctcgaac cggtccggat cgaccacccc gttcgggtcc atgtgaagca
     2581 tcatgtcgac gatcgcggag gagagatatt ccgcggtggc gaagccctgg ttgaaggtcg
     2641 acgccttctc gatcttggcg atcagcgccg ccggcatcgg ctgccccgtc ttgtaatgct
     2701 tggcgaagcg gctgagcacc ggctgggtca gaagccaatt ctcattcacc tggctcggat
     2761 attcgacgaa gtcgcgagga gtcccgccga agctcggata atagacgttc gacaggaagt
     2821 aatggatggc gtggccgaat tcgtggaaca gggtctcggc gtcgtcgagg ctgatcagga
     2881 ccggctggcc cggcgccggc ttgacgaaat tgttgttgtt ggagccgagc acgacgtcgt
     2941 cgccgagcag gccggcgcgg ctccgatagg tggtcgccca ggcgccggaa cgcttgcccg
     3001 tccgggcgta atcgtcgcgg tagaagacgc cgatctgctt gcccgacccg cggtcggtga
     3061 cgctgtagac gcggatgtcc ggatgccagg tcgggacggt gccggtgatc tccttgaaat
     3121 cgaggccata gagctcgccg gccgcccaat atgaggcctc gatgatgttg ttcagctcga
     3181 aatagggttt cacctcctcc tgggtgaggt tgtagcgctg cttgcgcacc ttctcctgat
     3241 agtagcgata gtcccagggc tcgatggtga ggctgcggtc ggtcttgcgg gcgagcgcct
     3301 gcatgtcggc gacctcttcc ttcacccggg cgacggcggc gggccagacc cggttcatca
     3361 ggtccagcgc cttggccggc gacttcgcca tcgtgtcctg catccggaac tcggcatggc
     3421 tggagaagcc gagcagcttc gcccggtcgg cgcgaagctt gacgatctcg gcgatgaccg
     3481 cgttggtgtc gttggcgccg ccattgtcgc cgcggttgac gaaggcgcgc cacaccttct
     3541 cgcgaagggc ccgattgtcg gcgaaggtca ggatcggatc gacggccgag cgggtgttga
     3601 cgatcgcgaa ggtcccggcg ggcagcttgc gctcctgcgc ggccgaggcg gcggcggcct
     3661 tcacgtcggc gggaacgccc ttcagctccg cgtcggtggc ggtgatgtag ctgctctcgt
     3721 cggccagcac cttctcgccg aagacggcga atttctgcgc cagctgcgaa ttgtaggcgg
     3781 tcagctgctg cttctcttcg ggcgacaggt tggcgccgcg gcggacgaag ctgtcgtaga
     3841 gccgggtgac caggcgctgc tgcttggcgt cgagattggc gttctcgcgc atctcgtaga
     3901 ccgtcttgat ccgctggaag agcttcgggt tgaggatgat ctcgtcgctc gcggccgaaa
     3961 gcttgggcga ccattccttc tcgagcgcct gataggcggg cgtcgtcatg ttgtcggtca
     4021 tgacggcgaa catcgagagg acccggtcga gccgctgtcc cgccttctcg agcgcctcga
     4081 cggtgttggc gaaggtcggg gcgtccgggc tgtcggcgat cgccagcacc tcgcggcgct
     4141 gctcgtcgat cccgaactgg atcgcctcgg ggaagagctc gggcttgacc tggtcccagg
     4201 gcggcacgcc gtcatagggg ccggtccagt cggcgagcag gacgttgtcc gggacgacca
     4261 cggccggctc ggccggggct tgggcgggcg cggccgcggc ggccgtggtc ggcccttcgt
     4321 cggcggcggt ggaggcgcag gcgccgaggc cgagcgcggc ggcggtggtg agcaggatgt
     4381 gcttcaagtc ttgtctccgg tcgggcggat cgggccggcg ggggcgcggc cgagcgatgg
     4441 cgccctatag cctccggcat gaaccgcccg caacttgtcc ggccttgcgc atttgccgcc
     4501 gcccccgact cgtttcggcg cgggaaagga atcgttcgcc gcaaatccct ttggaagcca
     4561 tggccttgga cctgtggata agtcagcctc gggcaaagtt cctgtcgggg gagtgacggt
     4621 gctcaacatc ctgatcgtcg aggacgatcc cttgctcgcc gagacggtga agagcctcgt
     4681 cgagctcaat ccgcgcttcc aggtgacggc gctcgccaac gatctcgcca gcgcggtcgc
     4741 cgcggtcgag gaccggcggc ccgacctcgc cctggtcgac ctgcagctcg ccaacggcag
     4801 cagcggctat gccgtcgccg cccggctgaa cgactacgga atcgcctgcc tgttcagcac
     4861 cgccaaggcg cccggcttcg ccgtccgcga cctcgccatc ggctgcctgc aaaagccgta
     4921 tcgcgaggag gacctggtcc gggcgctcac cgaggccgag gacatattga agggccgccc
     4981 caagctcgtc cttcgcccga gcctgcccga gccgctggaa atctatggcg aggaggacaa
     5041 ggccggcgag cccgccttcg ccctcgcgat cgggggccgc acgtcctggc tggcgaagct
     5101 caagaagctg gtgcgcaggc cgtcctcctt ccgaagcgct gcggcctgac gatattcctc
     5161 cccgcgattt cgcggggagg gggaccgcgc gaagcgcggt ggaggggttc ttgcgagctc
     5221 agcgggaacc cctccaccag gcttcgcctg gtgcctctcg cccgcaacgg cggcggagga
     5281 atgtcctcca tcgccaggcg cacgccctcg ggtccgaggg cctgtccttg accccgccat
     5341 aaggacagcc cggtgcctgg atcggccgcg ctcgtccgcc gcaagtccat caactcgctt
     5401 gaaagcctct cacgctttgg cacataatcg cctttcctga gggggtgtgt tttttccgat
     5461 gggggcgagc cgcaatcttg cgtccggcgc actggcggcc tcgctgctcc tcgcgctcgc
     5521 cgtcttcctg tctcccgccg gcgcccagca ggccgcggcc ggccattatg tcggagtcgc
     5581 gacctgctcg ggctcgacct gccacggccg cagcgaggcg accggcgcga tcgtccgcca
     5641 ggacgagctc cgcctctggc aggacccgtc gagccccgcc ggcgcccaca gccgcgcctg
     5701 gcgggtcctc accgagcctc gcgcccgggc gatcgccgcc cgcctcggca tcggcgaggc
     5761 ggccagcgcg ccgatgtgcc tcggctgcca cgccaccccc gcctccggag cgcgcgggcc
     5821 ccgcttccag atttccgacg gggtcggctg cgagagctgc cacggcgccg cctcgaactg
     5881 gctggcgagc cattacgcgg tcggcggcac ccactcggcc aacgtctcgc gcggcctggt
     5941 cccgctcgac gacccgcgcg cccgcgccgg gaaatgcctc gactgccatt tcggaagcgc
     6001 cggttccggc cagttcgccg atcatcggat gatggccgcc ggccaccccc ggatcagctt
     6061 cgagctcgac ctcttcacca ccctccagca gcatcatgac gaggacggcg actatgtccg
     6121 gcgcaagggg cgctcgaacg cggtcgccaa ttgggcggtc ggtcaggcga cggcgctcga
     6181 gcggtcgctg gccctgttcg cgactccggg gcgggggacg aacgggatgt tccccgaatt
     6241 ctatttcttc gactgccact cctgccaccg ccggatttcg gacgatcccc gcttcgagcc
     6301 gagcaaggcc gccaatccgg cacggccgat cccgtccggg actccgccct acaatgacga
     6361 gaacatgatc atgctcgccg ccgcggcccg ggtggcggcg ccccaggccg ctccgcgctt
     6421 cgagcgcgac agccggagat tccacgcggc gatgacccgc gaccgagcca gcgccctcgc
     6481 cgccgccgcg gcgctgaagg aaagcgcccg cgccgtcgcc gccgacctcg cccgcggagt
     6541 cagccgcgat caggtcttcg cgatcatcga cagcatcgcc ggcgacgcca tctcgccgcg
     6601 cttcaccgac tatacgggga gcgtccaggc ggtgatggcg gtggacaccc tgttctcctc
     6661 gcttggggcg ggcgctccgg agtcgatccg cgccgacatc aacggcgcct atcgcgcggt
     6721 gcgcgatccc aacgcctacg atccccgcga attccgagcg agcctgggcc gcgccgcggc
     6781 cgcgatccgg aagctgaaat gagacgcggg agggcattgg cctgcatcgc cgcgatggtc
     6841 gtctcgtcgt gcggcggcgg gggcggcgga agcccgccgc cgccgccgcc cccgccccct
     6901 gccccggcgc cccccgcccc gccggccggg gtctatccgg tgccggccca gctctcgctc
     6961 agccaggccg aggtccagca gatcgtcgct cggggagtcg cggaggcgca ggcccggtcg
     7021 ctgccggcca cgatcgcggt ggtcgaccgg gtcgggaacg tcctcgccgt cttcgccatg
     7081 aacggcgcga atccgcgcct gcagatcccg cgcgggccca acggcagcac ccacgacctc
     7141 cagggcgtcg acctgcccgc cgcggcggcc ggagcgatcg ccaaggcgat caccggcgcc
     7201 tatctctcgt cgggcgggaa cgccttctcg acccggacgg cgagcatgat cgtccaggag
     7261 catttcccgc cctcggccgc cgccaagggg cttgagagcg gcccgctgtt cggcgtccag
     7321 ttcagccagc tgccctgctc ggacctcgcc gcccgcttca acggcaatgc cggcgccggc
     7381 ggcttcatcg gcccgaagcg ctcgccgctc ggcctggccg ccgatccggg cggcttcccg
     7441 ctctacaagg acggggtcct ggtcggcgga gtgggtgtaa tggccgacgg cgtctacggc
     7501 ttcgaccccg aggtgcagga cgtggacaag gatcgcgagg aggcgatcgc ccttgccgcg
     7561 gcgaccggcc tcgccgcccc cgaggagatc cgcgccgagc ggatcagcgt cgacggcacc
     7621 ttgctgcgct tctccgacgc cgctccgtcc gacctgctgt ccaacccttc cgccgccccc
     7681 ggcttcgccg ccatcaacgg cagcgccggc gcgctcgcgt cggtgcgcgg ctattacgat
     7741 tcgaccatgg gcgtgctcgc gggccgcgcc tatgggacgg aggcgtcggg cgtccggccg
     7801 gcgacggcgg ccgagttccc caaccctgac gcgttcgtgc tgtcgaacgg cggcggctcc
     7861 aaccgcttcg ccgtccgagc cggcaccgac gggggcctca cggcggccga ggtgcaggcg
     7921 ctgctcgagg aagcgttcaa ggtgatgacg gcggcccgcg cgcagatccg gcgcccgctc
     7981 gacagccgcg cccaggtcag catctcggtg gtcgacagca gcggcgccgt gctcggcctg
     8041 gtccgctcgc cggacgcccc cgtcttcggg atcgacgtct cgctgcagaa ggcgcgcacc
     8101 gccgccttct tctcgagcgc gaatgcggcg gcgacgatcg aggcgagcgg cgactcggac
     8161 gtccgccagt tcgtcgcggc ggcccggacc ttcttcggca atcccgccgc cttcaccggc
     8221 agcttcgcct tcaccacgcg ggcgatcggg aacatctcgc gcccctattt ccccgacggg
     8281 gagctgggcc gcccgcccgg gcccttctcg cggccgatcg ccgagttcaa ccccttctcg
     8341 accggcctcc agtcggcgct ggtcgaggcc gacgtgaagg cccatgtcgg cttcgtcctc
     8401 ggcaacccgg ccgacacgcc ggcgcagtgc acccacacgc cgaggctcgc caacggcatg
     8461 cagatcttcc ccggctcggt gccgatctac cgcggcgcca ccctggtcgg ggcgatcgga
     8521 gtctccgggg acggaatcga ccaggacgac atgatctcct tcctgggagt gcacaatgcc
     8581 ggccttcgcg ccggcggctt cggcaatgcc gatccggccc gccgctccga ccagatcgtc
     8641 gtcccggtcg ggagcggcgt gcgcctccgc tacgtcggct gcccgttcgc gcccttcctg
     8701 ggatcgaacg agcagaatgt gtgccagggc aagtgagctt cggtctggtc ctgggcctgc
     8761 tggccggcgc gagcgggatc gccctgcaat ccccgtcccc ccagccaagg ccggatgacg
     8821 agccgggggt tcaagaccgg ccgacgcagg aggagccgga ccctctcccc aacatcccca
     8881 ccgaggaagg ccgccgccgt cccggaatcc cgcagagcga gctcgacgac ccggtcagcc
     8941 aggacaatcc cggcgccatc cgggcgccgc cgccggaagc cttcccgacc gaccagatcc
     9001 ccgtcccgga ccgctggcgg ctgatcgaga gcctcggcgt ggtcaaggag cgctggttcg
     9061 acccctacaa ccagaacagc tacaagggcg accggcccct ctgcctccca acgccggagg
     9121 agcaggcgag gcgccgcgcc gaaggccggc cgcgctgccg gacgccgcgc ttcctcggcc
     9181 tcgacgagga cgattggttc ctcgtcgtca acgcggtctc cgacacggtc gtcgagccgc
     9241 gcaccttccc gatcccggtc ggcgttcaga cgacggcggg gccggggagc ctcgacgtct
     9301 tcggcaagaa cgacagcctg gccctgtcgc agacgttcat cgccggagcc gcgctggtca
     9361 agggctcgac cgcctacatg ccgccccatg tcgaatatcg gctgacgctg gcgctcaacg
     9421 tcaattacgt cgacgtcgcg gagcgccggg tcctgttcgt cgagccctcg aagggcacca
     9481 gccgcctcga ttacgcgctc ggggtccagg aggcgttcgt cgactatcac ctgcgcaacg
     9541 tctcggaccg ctacgatttc gacagcgtga gggtcggcat ccagcccttc cagctcgatt
     9601 tccgaggctt cctgttcaac gaccagcagc tcggcgtccg gctgttcggc aatcgccgca
     9661 gcaatcgctt ccaatacaat ctgatggcga tctggcggct cgagaaggac acgaacagcg
     9721 gcctcaacaa cgtcctccag tcgccgcgca aggactggat cctccacgcc aacctctatc
     9781 gccaggattt cccgttcgtc ggcctgacca gccaggcgag cgtcaccgtc aacctcaacc
     9841 gcgagcgcgg cgacatcgag gtggacgaca acggcttccc ggtccggccg gcgctgatcg
     9901 gcaaccttcg agcgcgcaat tacgaggtcg cctatctggg ctacagcgcc gacgggcgga
     9961 tcggccggat caacctctcc gcctcggcct atggcgcgtt cggctgggac cggaacaaca
    10021 tcttcaccgg ggagaccggc aagatccgga gcttcttcct cgccgccgag ccgagctacg
    10081 acctgaactg gatgcgcttc cgcggcgcct tcctcttcgc gagcggcgac ggcgagccgt
    10141 tcgacgaccg cgaaggcggc ttcgacgcga tcttcgagaa cccggtcttc gccggcgccg
    10201 acaccagcta ttggatccgc cagtccgtcc ccttcgccgg cggcggccgc gccgtctcga
    10261 tcaacggccg caacggcatc ttgaactcgc ttcgctcgtc caaggagcag ggccagtcca
    10321 acttcaacaa tccgggcacc atcctgatcg gcgccggagc cgatttcgac ctcacccccg
    10381 agcttcgggt gagcggcaac gtcaatcacc tctggttcca cgagaccgcg gtgctgcagg
    10441 cgctgcgcaa cgagggctcg atcccgcgcg acatcggctg ggacgtctcg gccgccgcga
    10501 tctggcgccc gaagatgacc cagaacatcg tcttgcgcgc ctcggcggcc gtgcttcagc
    10561 ccggcgccgg cttccgcgac ctcttcttca actcggaccg cgacaagcgt tactattcgg
    10621 tcctcttgaa cgcgatcgcg acctattgat gcggtggccg cggcgcctcc gcttccaccg
    10681 ccaccccggc gaaggctggg gtccagggag cgtcgatccc cgcacaagct cggcatggat
    10741 tccagctttc gccggaatga cgatgtttct gggactcctc gccacccccc tgctcgcgtc
    10801 ggaaggcggg aagcccgtcg cccgcgacca tgccctggtc cgcgccgggc ccgcgccgcg
    10861 aacccaggaa tgggactcgg ccgaggcgaa gagcgcgggc tgcgtctcct gccacgtcgg
    10921 cagcgaccag aagacgatgc acgcctcgcc cgcggtcgtg ctcggctgcg tcgactgcca
    10981 cggcggcaat gcctcggtcc agggcgatgc ctcgcttcct cacgacggcc cggcctatgt
    11041 cgccgtccgc gacaaggcgc atgtcctgcc ccgatacccc aaagcctggc actggccgtc
    11101 gagcgccaat ccgaagcgga gctatgccct gctcaacgcc gagagcccgg aatatgtgcg
    11161 cttcgtcaac ccgtccgact atcgggtcgc ccgcgattcc tgcggcgcct gccatatcga
    11221 gacgatcgag gccgccgagc gctcgctgat ggcgtcgggg gcgatgctgt ggggcggcgc
    11281 cgcctacaac aacggcatcc tgccctacaa gaactacctg ttcggcgaag cctacacgcg
    11341 ccacggcgag ccggcgagaa tcgtctcgcc gccctcgccc gaggcggtca ctccggagca
    11401 gaaggcgcgc ggagtgctcg ccgagctcta cccgctgccg acctggcacg tcgtcccgcc
    11461 gggcgacgtc ttccgggtgt tcgagcgcgg cggccgaacc gtcggcaccc agttccccga
    11521 gatcggcctc ccctcgccct ccgggtcgat ccagcggctg gaggaggccg gccggcccga
    11581 catccgccag tcgaaccgcg gtcccggcac cggcctcagg gtcgccatcc cggtgctcaa
    11641 catccacaag acccgtttga acgacccctt catgtggttc atgggcacca acgaccagcc
    11701 cggcgactat cgccattcgg gctgcgcggg ctgccacgtc gtctacgcca acgaccgcga
    11761 gccgaggcac agcctcacct acgcccagtt cggccgcgac ggtcagacgg cgacggcgga
    11821 tccgacgatc gcggggaaga cggagcatcc cggctccccg accgcggcgg cggcccccgc
    11881 ggacgcggcc cacgggtcgc tgaaggagaa gggccatccg atccgccacg ccttcacccg
    11941 cgccatcccg accgcccagt gcatgacctg ccacatgcac cagcccaaca tcttcctgaa
    12001 ctccttcctc ggctacacga tgtgggatta cgaggccgat gcggacctga tgtggcccgg
    12061 gccggagaac cggctgccga ggaaggacgg ggaggagcgg agcctcggcc gccagcgcta
    12121 tcccgacgcc gcccaggccc tcgaaatcct cgaccgcaac cccgaggccg ccgcgacccg
    12181 cgggctgtgg ggggacgtgg acttcctgcg cgaggtcaat gaccgggtga acccgctcgc
    12241 ccgccatacc cagttcgccg actatcacgg ccacggctgg aacttcaggg ccgtctacaa
    12301 gcgcgaccgc gagggcaatc tgctcgattc cgacggcaag gtcgtcgccc cggacgatcc
    12361 ggagaagttc ggccggaccg gctcgcccca gttcgtcccg cccggcgagc agaagggcaa
    12421 ggcggtccac atgatggaca tccacgccga gaaggggatg caatgcgccg actgccattt
    12481 cgcccaggac agccacggca acggcttcct ctacggcgag gtcgccaatg cggtcgagat
    12541 cggctgcaag gactgccacg gcaccgccgg ccgatatccg aacctgctca cctccggccc
    12601 ggctgcgccg ccgaggggga ccaacctcgc gctgctccgc aaccaggacg ggcggcggcg
    12661 gttcgagtgg atggcgggtg aggacggccg gcgggtgctg gtccagcgct cgatcgtcga
    12721 cccggcgctg gagtgggagg tgtcgctggt gaaggacagc ctcgacccgc ggtttccagg
    12781 cttcaacctc aaggcggcgc gggccaagct gatgtcgcgg gaagggtcgg aaagcggccg
    12841 ctatgttttc ggatcgggaa tcgcgtccgg gaatttggcc cacaaggacg atgagatggc
    12901 ctgcttcacg tgccatctga gctggacgac gagctgcgcg ggctgccatc tgccgatcga
    12961 ggcgaactgg aagaccaaga gcaacagata cgattcgtcc gagacccgca atttcgccac
    13021 ctacaacccg caggtcgcgc gcgacgacat gttccagctc ggccgccatc agacgacgaa
    13081 gggcaatatc gtcgcgccgg tcaggtcgag ctcggcgctg gtcttgagct cgaccaacat
    13141 caaccgcgaa aggatctacg tccagcagcc gccgatcagc gcggccggct tttcgtccca
    13201 ggctttcgcg ccgcattttc cgcacaccgt gcgcaagacc gagacgaaaa cctgcacgga
    13261 ctgccattta tcgcagcagg acgacaacaa tgcggtcatg gcgcagctgc ttctgcaggg
    13321 caccaatttc gtcaatttcg tcggccttca cgcctatgtc ggcctcgatg gcggcttcca
    13381 ggcgacccgg gtgaccgagt gggacgagcc gcaggcggtg atcggctcct atttgcaaag
    13441 atttgcatac cccgatttct ggcgccagca tgtcgaccgc aacaagcggg agctgacgaa
    13501 ctggacccgc ggcggccccg gctcgcgcga acagtttgca aatattttcc agggcacggg
    13561 cggcgccgtc ggctgcctcc agcagcgcgg cgaatatatg ttcgtcgccg aagccagggg
    13621 cggcttcagg gtctacgaca tagccagcct cggcaacaaa ggcgtttcgg agcggatcgt
    13681 cagcgccccc ttctcgccgc tcggccagga tatgaaggtc gcctcgagaa acgccacctg
    13741 cctggcgctt ccgaccaacc agccgatcag cgtcgggcgg aacgacaagg tcgtgaagaa
    13801 catgcccgaa aaccaggagc aggccttcca tccgatctac cgttacgcga tcatcaccga
    13861 ttccgaggaa ggcctcatcc tggtcgacgt cgagactttt gccgacggcg agccccgcaa
    13921 caacttcatc aagcgggcgg tgacttggaa cgagggcggt gttctcaacg gtgcgcggca
    13981 catcacg
//
LOCUS       DASVQZ010000286         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_58656, whole genome shotgun sequence.
ACCESSION   DASVQZ010000286 DASVQZ010000000
VERSION     DASVQZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58656"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF547_07845"
     CDS             <1..309
                     /locus_tag="VF547_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HEX8262770.1"
                     /translation="LAELGFGLDLSECAATGVRERLAFVSPKSGRAVSEEGAGAYRDR
                     LLALPAFLRAGGDGGWDDIFDGLKLTGHFLARDLLIERQADILAARERLVERLRRVTR
                     "
     gene            343..741
                     /gene="apaG"
                     /locus_tag="VF547_07850"
     CDS             343..741
                     /gene="apaG"
                     /locus_tag="VF547_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX8262771.1"
                     /translation="MKALFPHAVTTQDVTVRVSVSFLPEQSDPGKGRWFWAYHVRIEN
                     GGRRAVQLISREWVIVDGRGGRHEVQGEGVVGEQPVIEPGASFDYVSGCPLGTPTGRM
                     EGRYHMVGEEGASFSVAIPSFPLIAPAVTS"
     gene            complement(738..>1671)
                     /locus_tag="VF547_07855"
     CDS             complement(738..>1671)
                     /locus_tag="VF547_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262772.1"
                     /translation="KSIGRNGDEAARLPIPVAGLYRANGRVAAHLGEGEDYHRSAYRS
                     WTILKEGADVPEWQRLAARLEVANMISSLQGLWAGRRAYLQLAEEADRAGRADIAAIA
                     RLKAIWAEYVATPTRPVEKQLWRIANEANPATRQSRLGALILLARIERDRKSKQGAAA
                     EALIKELSATRSTRPTLLYAPEISQLRGDKWNPDPSAVRGYSVTQRMAAENFDDRWVD
                     IAFRIEPNGRVADVEIVRESGSTRWAEPVLGAIAGRVYAPLADGQSQYRLERFTWTSN
                     WDTRIDTRLRVRSPEGVIQSLDLTAGPPPAAKGS"
BASE COUNT          220 a          570 c          605 g          276 t
ORIGIN      
        1 ctcgcggagc tcggcttcgg gctcgatctg tccgaatgcg cggcgaccgg cgtgcgcgag
       61 aggctcgcct tcgtcagccc caagagcggg cgcgcggtca gcgaggaggg ggcgggggcc
      121 tatcgcgacc gcttgctcgc gctgcccgcc ttcctgcgcg ccggcggcga cggcggctgg
      181 gacgatatct tcgacgggct gaagctcacc gggcatttcc tcgcccgcga cctgctgatc
      241 gagcgccagg cggacattct cgccgcgcgc gaacggctgg tggagcggct gcggcgggtc
      301 actcgttgac gcggcgaagc ggatattgga aggctccccg ccgtgaaagc attgttcccc
      361 cacgcagtca ccacgcagga cgtcaccgtc cgcgtctcgg tgagcttcct ccccgagcag
      421 tcggaccccg gcaagggccg ctggttctgg gcctatcacg tccggatcga gaatggcggc
      481 cggcgcgcgg tccagctgat cagccgcgaa tgggtgatcg tcgacggccg cggcgggcgc
      541 cacgaggtgc agggcgaggg ggttgtcggc gagcagccgg tgatcgagcc gggcgcgtcg
      601 ttcgactatg tctcggggtg tccgctcggc acaccgaccg gccggatgga gggccggtac
      661 catatggtcg gcgaggaggg cgccagcttc tccgtcgcga tcccgagctt cccgctgatc
      721 gcgccggcgg tgaccagcta gctccccttg gcggccggcg gcggaccggc ggtcagatcg
      781 agactctgga tcacgccctc gggcgagcgg acgcgcagcc tggtgtcgat gcgggtatcc
      841 caattgctgg tccaggtgaa gcgctcgagc cggtactggc tctggccgtc ggcgagcggg
      901 gcgtagaccc ggcctgcgat cgcgcccagc accggctcgg cccagcgggt ggagccgctc
      961 tcgcggacga tctcgacatc ggcgacgcgc ccgttcggtt cgatccggaa tgcgatgtcg
     1021 acccagcggt cgtcgaaatt ctcggccgcc atgcgctgcg tcaccgaata gcccctgacg
     1081 gcgctcgggt ccgggttcca cttgtcgccg cgaagctgtg agatttccgg ggcgtaaagc
     1141 agcgtcggcc tggtcgacct ggtcgccgac agctccttga tcagcgcttc ggccgccgcg
     1201 ccctgctttg acttgcgatc gcgctcgatc cgcgcgagca ggatcagcgc gcccagccgc
     1261 gattgcctgg tggccgggtt ggcttcgttc gcgatccgcc acaattgctt ttcgaccggg
     1321 cgggtcggcg tggcgacata ttcggcccag atcgccttca gccgggcgat ggcggcgatg
     1381 tcggcgcggc cggcgcgatc ggcttcctcg gcgagctgga ggtaggcgcg cctgcccgcc
     1441 cacagcccct gcaacgagga gatcatgttg gcgacttcca gccgcgccgc gagccgctgc
     1501 cactccggaa cgtcggcacc ctccttgagg atggtccagc tgcggtaggc cgatctgtga
     1561 taatcctcgc cttcgccgag atgggcggcg acccggccgt tggcgcggta gaggcccgca
     1621 acggggatcg gcagcctcgc ggcctcgtcg ccgttgcggc cgatcgactt c
//
LOCUS       DASVQZ010000287         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_348325, whole genome shotgun sequence.
ACCESSION   DASVQZ010000287 DASVQZ010000000
VERSION     DASVQZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348325"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VF547_07860"
     CDS             <1..1130
                     /locus_tag="VF547_07860"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX8262773.1"
                     /translation="VSPENPAGAKGAGARENRGAKGHAFETIPIGGAHVLADIRGAGT
                     IDRMWLTIEDRSPEALRGLKLEMFWDGAAAPAVSVPLGDFFLHGAGEMVPMETALLAS
                     PEGRSFVSYIPMPFRTGARLVVTNESGKPVNLIFYDIDYRALPRQPKDALYFHAGWSR
                     ERATTLARDFRILPRISGRGRFLGAAVTVLTNPAYEKTWWGEGEVKIALDGDRSTGPT
                     LVGTGTEDYIGTAWGQGAYINRYQGAPVASWEGEGRWTFYRFHIPDPIFFARDIEVSL
                     QMMGGARKAVVLGLQKKGVPLIPVTIDPGSRVNFQQLLARNPPLLLSDPSLPDGHTNF
                     YRSDDVAAVAYFYLDRAEGVLPKIAPAQERLTALRPPPERK"
     gene            complement(1174..>2279)
                     /locus_tag="VF547_07865"
     CDS             complement(1174..>2279)
                     /locus_tag="VF547_07865"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8262774.1"
                     /translation="GRSFDINEKSYAAYGQLQYEIPLGGEASIDGTIGLRVVKTEEDI
                     SGALSAPGRPVFPVNFQNEYTDWLPNANMRIRFTPQLQLRLAATETRTRPTFQQLNPA
                     LSLSQPPTCTPGVRCERTGSGGNPFLEPLTSTNYDASLEYYFSRTGFASVAAFRRNMK
                     GFVITQTAELPEPDPETGAVLLVTAPFNSERGRIQGLEAQVSTFFDFGGVPDFLRSFG
                     IQANATYIDAKTDQPVPGPAGFTRRLRIPDVSKWTYNLVGMYEGGGLTLRLAYNRRTG
                     FPEGALSNRGDYTLQGRGHPVSRLDWSSSYAFSDNFTVFFDATNLLGKPFKSDIVRVN
                     YNPTTGAVTGGEAFPMLVRYEETVFTGGFRFRF"
BASE COUNT          367 a          776 c          773 g          363 t
ORIGIN      
        1 gggtgagccc ggaaaatccc gccggcgcca aaggggcggg agcccgcgag aatcgcggcg
       61 ccaagggcca tgcgttcgag accatcccga tcggcggcgc gcacgtcctg gcggatatca
      121 ggggcgccgg caccatcgac cggatgtggc tgacgatcga ggaccggtcg cccgaggcgc
      181 tgcgcgggct gaagctcgag atgttctggg acggcgccgc ggcgcccgcc gtctcggtcc
      241 cgctcggcga cttcttcctc catggcgccg gcgagatggt gccgatggag acggccctgc
      301 tcgcaagccc ggaaggccgc tccttcgtct cctatatccc gatgccgttc cgaacgggcg
      361 cgcgcctggt cgtgaccaac gagtcgggca agccggtcaa cctcatcttc tacgacatcg
      421 actaccgcgc cctcccccgc cagccgaagg acgctctcta cttccacgcc gggtggagcc
      481 gggagcgcgc gaccaccctg gcccgcgatt tccggatcct gccgcgaatc agcggccgcg
      541 gccgcttcct cggcgccgcc gtcaccgtcc tcaccaatcc ggcctacgag aagacctggt
      601 ggggggaagg cgaggtcaag atcgcgctcg acggcgatcg ctcgaccggg ccgacgctgg
      661 tcggcacagg caccgaggat tatatcggca ccgcctgggg ccagggcgcc tatatcaacc
      721 gctaccaggg cgccccggtc gccagctggg aaggcgaagg ccgctggacc ttctaccgct
      781 tccacatacc ggacccgatc ttcttcgccc gcgacatcga ggtcagcctg cagatgatgg
      841 gcggcgcccg caaggcggtc gtgctcggcc tccagaagaa gggcgtcccg ctcatcccgg
      901 tgaccatcga cccgggctcc cgcgtcaatt tccagcaact gctggccagg aacccgccgc
      961 tgctgctttc ggacccttcg cttccggacg ggcacaccaa tttctaccga agcgacgacg
     1021 tcgccgccgt cgcctatttc tacctcgacc gggccgaagg cgtcctcccc aagatcgcgc
     1081 cggcgcagga gcgcctgacc gcgctgaggc cgccgcccga gcggaaatag cggccgtcct
     1141 tgcgggcgaa gcgccgctcc ccgcgacgac gctctagaag cggaagcgga atccgccggt
     1201 gaacaccgtt tcctcgtagc ggacgagcat cgggaacgcc tcgccgcccg tcactgcgcc
     1261 ggtcgtcggg ttgtagttca cgcgcacgat gtccgacttg aagggcttgc ccagaaggtt
     1321 ggtcgcgtcg aagaacaccg tgaaattgtc gctgaaggca tagctgctcg accagtccag
     1381 cctcgaaacg gggtgaccgc gtccctgcag cgtgtagtcg ccgcgattgg acagggcgcc
     1441 ctccgggaag cccgtgcggc gattgtaggc gagacgcagg gtgagcccgc cgccttcgta
     1501 catgccgacg aggttgtacg tccatttcga cacgtccggt atgcgcaggc gccgggtgaa
     1561 gcccgccgga cccggcaccg gctgatcggt cttggcgtcg atgtaggtgg cgttcgcctg
     1621 gatgccgaag ctcctcagga agtcggggac gcctccgaag tcgaagaagg tgctcacctg
     1681 cgcctcgaga ccctggatcc ggcccctctc gctgttgaag ggcgccgtga ccagcaggac
     1741 ggcgccggtc tccgggtcgg gctccggcag ctccgccgtc tgggtgatga cgaagccctt
     1801 catgttccgc cggaaggcag ccaccgaggc gaagcccgtc cgcgagaaat aatattcgag
     1861 gctcgcatca taattggtcg aggtgagcgg ctcgaggaag gggttgccgc cgctgccggt
     1921 gcgttcgcag cgcacgcccg gagtgcaggt cggcggctgc gaaaggctga gcgccgggtt
     1981 cagctgctgg aaggtcggcc gggtccgagt ctcggtggcg gccagccgca gctgcaattg
     2041 gggcgtgaag cggatgcgca tgttcgcgtt gggcagccag tccgtatatt cgttctggaa
     2101 gttgaccggg aagaccggcc ggccgggtgc ggacagggcg ccgctgatat cctcctcggt
     2161 cttgaccacg cgcagcccga tcgtgccgtc gatgctggcc tcgccgccca acgggatctc
     2221 gtactggagc tgaccgtagg cggcgtagga cttctcgttg atgtcgaacg accggcccg
//
LOCUS       DASVQZ010000288         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_349069, whole genome shotgun sequence.
ACCESSION   DASVQZ010000288 DASVQZ010000000
VERSION     DASVQZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349069"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /gene="scpA"
                     /locus_tag="VF547_07870"
     CDS             <1..1596
                     /gene="scpA"
                     /locus_tag="VF547_07870"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX8262775.1"
                     /translation="NDILKEFAVRNTYIYPPGPSMRIVADIIAYTSEHMPRFNSISIS
                     GYHMLEAGATAVQELAFTLADGMEYVRVARARGLEVDAFAPRLSFFFGIGMNFFMEVA
                     KLRAARMLWHRIMDGFGARNPKSLMLRTHCQTSGVSLTEQDPHNNIVRTTIEAMAAVL
                     GGTQSLHTNSFDEAIALPTDFSARIARNTQLILAEESGITHVADPLGGSYFVESLTKE
                     LADRAWALIEEVEAMGGMTAAVAEGLPKRRIEEAAAARAARADRGEDVIVGVNRYRLQ
                     DEAHLDILQIDNDKVRAGQVARLETVRSERDEAATKAALDALREGAKGDSNLLALSVD
                     AARARATLGEISQALEDVFGRYGTTPEPVKGIYGGAYQGDPRWARAGAGVEAIGRRLG
                     RKPRILVAKMGQDGHDRGANLVASAFADLGFEVVPGPLFQTPEESARLAVEEEVDLVG
                     ASSLAAGHKSLVPELIGHLRAMGRPDIKVVVGGVIPPQDYDMLREAGVQAIFGPGTNL
                     VDAAGEVLKLLGHNMPPLEEAAE"
     gene            1620..>2945
                     /locus_tag="VF547_07875"
     CDS             1620..>2945
                     /locus_tag="VF547_07875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8262776.1"
                     /translation="MFSKILIANRGEIACRVIRTARRMGIRTVAVYSDADARAPHVKL
                     ADQSVRLGPPPASESYLNAELIIAACRETGAEAVHPGYGFLSERESFARALEEAGIAF
                     IGPPAGAIAAMGDKIESKKLAREAGVSVVPGFVGEIEDTEHAVRIAAEIGYPVMMKAS
                     AGGGGKGMRLAWSETDVREGFEATKREGLASFGDDRVFIEKFVENPRHIEIQLIGDKA
                     GNLLYLGERECSVQRRHQKVVEEAPSPFVTPEMRKAMGEQAVALARAVGYYSAGTVEF
                     IAGADRSFYFLEMNTRLQVEHPVTEQITGQDLVEWQLRVASGEPLPLTQDQLRIRGHA
                     MEARLYAEDPAHEFLPSTGRLDHFELGRRIRIDTGVEEGDRISPYYDPMIAKLIARGN
                     SREEAIDTLLDALEQVEVWPVKTNAAFLARTAADPDFRAGEVDTAFIPSK"
BASE COUNT          489 a         1005 c         1030 g          421 t
ORIGIN      
        1 aacgacatcc tcaaggagtt cgcggtccgc aacacctaca tctacccgcc cgggccctcg
       61 atgcggatcg tcgccgacat catcgcctac acgtccgagc acatgccgcg gttcaactcg
      121 atctcgatca gcggctacca catgctcgaa gcgggggcga cggcggtgca ggagctcgcc
      181 ttcacgctcg ccgacgggat ggaatatgtc cgcgtcgccc gcgcccgcgg cctcgaggtc
      241 gacgccttcg cgcctcggct gagcttcttc ttcggcatag gcatgaactt cttcatggag
      301 gtggcgaagc tgcgcgcggc gcggatgctg tggcaccgga tcatggacgg cttcggcgcg
      361 aggaacccca agtcgctgat gctgaggacg cactgccaga cgtcgggcgt gtcgctgacc
      421 gagcaggacc cgcacaacaa catcgtccgc accacgatcg aggcgatggc ggcggtgctg
      481 ggcggcaccc agtcgctcca caccaacagc ttcgacgagg cgatcgcgct ccccaccgat
      541 ttctcggcgc ggatcgcccg caacacccag ctgatcctgg ccgaggaaag cggcatcacc
      601 catgtcgccg atccgctcgg cgggtcctat ttcgtcgaga gcctgacgaa ggagctggcc
      661 gacagggcct gggcgctgat cgaggaggtg gaggcgatgg gcgggatgac cgccgcggtc
      721 gccgagggcc tgcccaagcg gcggatcgag gaggcggcgg cggctcgggc ggcgcgggcc
      781 gaccggggcg aggacgtgat cgtcggggtg aaccgctacc ggctccagga cgaggcgcat
      841 ctcgacatcc tccagatcga caacgacaag gtccgcgccg gccaggtcgc gcggctggag
      901 acggtgcgct ccgagcgcga cgaggccgcg accaaggcgg cgctcgacgc ccttcgcgag
      961 ggggcgaagg gagattccaa cctgctcgcc ttgtcggtcg acgcggcgcg ggcgagggcg
     1021 accttgggcg agatcagcca ggcgctcgag gacgtcttcg gccgttacgg caccacgccc
     1081 gagccggtga agggcatcta cggcggcgcc tatcagggcg acccgcgctg ggcgcgcgcg
     1141 ggcgccggag tcgaggcgat cgggcgccgg ctcggccgca agccgaggat tctggtcgcc
     1201 aagatgggcc aggacgggca cgaccgcggc gccaacctcg tcgcctccgc ctttgccgac
     1261 ctgggcttcg aggtcgttcc cgggccgctg ttccagacgc ccgaggaaag cgcgcgcctc
     1321 gccgtcgagg aagaggtcga cctcgtcggc gcctcgagcc tcgccgccgg ccacaagagc
     1381 ctggtgcccg agctgatcgg ccaccttcgg gcgatgggcc ggccggacat caaggtcgtc
     1441 gtcggcgggg tcatcccgcc gcaggattac gacatgcttc gcgaggcggg ggtgcaggcg
     1501 atcttcgggc cgggcaccaa cctggtcgac gcggccggcg aggttctcaa gctcctcggc
     1561 cacaacatgc cgccgctcga ggaggccgcg gaatgacggt tcattgggta caaggcgcca
     1621 tgttctccaa gatcctgatc gccaatcgcg gcgagatcgc ctgccgggtc atccgcaccg
     1681 cgcgcaggat ggggatccga accgtggccg tctattcgga cgccgacgcc cgggcgccgc
     1741 atgtgaagct cgccgaccag tcggttcggc tggggccgcc gcccgcgtcg gaaagctatc
     1801 tgaacgccga gctgatcatt gccgcctgcc gggagaccgg cgccgaggcg gtgcaccccg
     1861 gctacggctt cctctccgag cgcgagagct tcgcccgggc gctggaggaa gccggaatcg
     1921 ccttcatcgg cccgcccgcc ggagcgatcg ccgccatggg cgacaagatc gaatcgaaga
     1981 agctcgcccg ggaggcgggg gtcagcgtcg tccccggctt cgtcggcgag atcgaggaca
     2041 ccgagcatgc ggtgaggatc gccgccgaga tcggctatcc ggtgatgatg aaggcgtcgg
     2101 ccggcggcgg cggcaagggg atgcggctcg cctggtccga aaccgacgtc cgcgagggct
     2161 tcgaggcgac caagcgcgag ggcctcgcca gcttcggcga cgaccgggtg ttcatcgaga
     2221 agttcgtcga gaatccccgc cacatcgaga tccagctgat cggcgacaag gccggcaacc
     2281 tcctctatct cggcgagcgc gaatgctcgg tgcagcggcg ccaccagaag gtggtcgagg
     2341 aggcgccgtc gcccttcgtc acgccggaga tgcgcaaggc gatgggcgag caggcggtcg
     2401 ccttggcgcg ggccgtcggc tattacagcg ccggcaccgt cgagttcatc gccggcgccg
     2461 accgaagctt ctacttcctc gagatgaaca cccggctcca ggtcgagcat ccggtgaccg
     2521 agcagattac cggccaggat ctcgtcgaat ggcagctgcg cgtcgcgtcc ggcgagccgc
     2581 ttccgctcac acaagaccag ctccggatcc gcggccatgc gatggaagcc cggctctatg
     2641 ccgaagatcc ggcgcacgaa ttcctgccgt cgaccggccg cctcgaccat ttcgagctcg
     2701 gccgccggat ccggatcgac acgggcgtgg aggaggggga ccgcatcagc ccctattacg
     2761 atccgatgat cgccaagctc atcgctcgcg gcaactcgcg cgaggaggcg atcgacaccc
     2821 tcctcgatgc gcttgagcag gtcgaggtct ggccggtgaa gaccaatgcc gcctttctag
     2881 cgcgcaccgc cgccgacccg gatttcaggg ccggcgaagt cgacacggcc ttcatcccgt
     2941 cgaag
//
LOCUS       DASVQZ010000289        11092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_735417, whole genome shotgun sequence.
ACCESSION   DASVQZ010000289 DASVQZ010000000
VERSION     DASVQZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735417"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2644)
                     /locus_tag="VF547_07880"
     CDS             complement(<1..2644)
                     /locus_tag="VF547_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8262777.1"
                     /translation="MRNISSWAIRNPVFPLVLFAALSLLGLVSFLRMDVNNNPDISFP
                     AATVTVVQPGAAPSELETQVTQRVEAAIRGINGVDEIQSTAREGSSTTFVQFDMGTPV
                     DRAVNDVRDAIANIRSDLPDGILEPQVQREEADGGPIAYMAVESTAMGLEELSWYIDN
                     TISKRLLAIPGMANVSRGGGVSREIRIILDPVKMQAHGITAAQVNAQLRQTNVNAAGG
                     RAEIAGSEQSVRVLGNAVDARDLGEKNISVGNGRTIKLSSIATVRDLYAEQRNVALMN
                     GRQVLSFSIEKAKGSSDVTVWDAAEKEMEKLEKESGGKVKFRLLFTSVQYTEGQYHSA
                     MEALVEGAVLAVLVVLLFLRDWRATLISAVAIPLSAIPTFWFMDLMGFTLNGVTLLAL
                     SLVAGVLVDDAIVEIENIVRHMRMGKSAYQAAIDAADEIGLAVVATTFSIVAVFLPVG
                     MMGGLIGQYFKSFGMTIVVAVLISLLVARLITPLMAAYFLKAKGSASHGEGWLMDRYM
                     GLLWWSLKHRWKTMLIGVAAFIATIFAFGTLPFTFQPNVNSDFSSVAVEMAPGSTLQQ
                     TQRIASQAADIIRKEPDVVKAFASIRVGGANIYITLSDDRKRTSSEFERQTAPKLRQI
                     ADARVYFRSQQGFGNRDVGIMLSGDDPVKLEQAANALVQDMKKMPEVKAPRIEGDLRR
                     PEIVIKPRFDLAADMGVTTAALSQTIRIATQGEIDQNSAKFSLSDRQIPIRVALNEES
                     RQNLSTIENLPVPTTSGGSVPLKVVAEIGFGAGPTQIQRFNQQRRIVVGADLAPGVVE
                     AMSKIKQLPSYNNLPAGVEHARQGEQKMQQELIENFIMAVVAGIFLVFAVLVLLYKRI
                     VPPFVNMGSLLLAPLGGAIALHLTG"
     gene            complement(2672..3880)
                     /locus_tag="VF547_07885"
     CDS             complement(2672..3880)
                     /locus_tag="VF547_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8262778.1"
                     /translation="MNFESPKWRRGALEKDVVVVDEETAARRRRRNIILAIVAVLVLL
                     AVAYLMFGRGGEDAKVAAGGKAGESAEAAPAVSVVVPGRQQVARTISATGQLAARYDM
                     PVGIPGEGGKVVNVLVEAGQWVGAGQTLAVIDRSVQTQEAAQLAAQIDVARADARLAQ
                     QELDRAQKLVARGFVSKADVERRIAARDAALARVRVAQANLGQSRARIGRLNVTAPAA
                     GLVLARSVEPGQIVGPGSGALFRIARGGQYEMLARLPEADLVTLSAGIPAQVTPVGSA
                     KTYQGSIWQVSPVIDPASRQGVARIAVPYDRDLRPGGFATANIRAGSVDAPMLPESAV
                     LNDSQGSYVYIVDKDNAVRRRPVKVGSVTDEGVVIAEGLNGTERVVLSAGPFLTEGEK
                     IRPQRAVAAR"
     gene            4080..4346
                     /locus_tag="VF547_07890"
     CDS             4080..4346
                     /locus_tag="VF547_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8262779.1"
                     /translation="MNLLADYGWLVWIILGAIAGGIAKAIMPGRDPGGCIVTILLGIA
                     GAALAGFIGNQLGWYEQGEGAGFIAAIVGAILILFIWRLIAGRR"
     gene            complement(4343..5131)
                     /gene="trpA"
                     /locus_tag="VF547_07895"
     CDS             complement(4343..5131)
                     /gene="trpA"
                     /locus_tag="VF547_07895"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX8262780.1"
                     /translation="MTRYAAMFDRLRRRGEGAFGAFAMLGDPDPAASASILDAAVEAG
                     ADMVEVGIPFSDPVADGPVIQAAAVRALAAGTRTGDCFGLLAQFRQRHPDVPVGILTY
                     ANIVVARGREAFYRAAAAAGVDSVLVADVPMLEAEPFLAAARGAGVAPVLIAAPNTPP
                     DRLRRIAEAGEGYTYCVARAGVTGADAQVRFAGEAIAALKAAGAPPPVLGFGIARPDH
                     VRLALQAGAAGVISGSAIVDRIARGEDVRPFVAEMKAATRSRDA"
     gene            complement(5128..6303)
                     /gene="trpB"
                     /locus_tag="VF547_07900"
     CDS             complement(5128..6303)
                     /gene="trpB"
                     /locus_tag="VF547_07900"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX8262781.1"
                     /translation="MRLSGRFGAYGGAYVPEILVPAIEQLEAAFLDAAGDPSFQAELD
                     GLLAHYAGRPTPLTRCRNLGSERVRIYLKREDLLHGGAHKTNQVLAQALLAKRMGKRR
                     LIAETGAGQHGVATALAGALFGLETRIYMGALDVERQRANVFRMELMGAEVVPVESGS
                     RTLKDAINEALRDWAASFGETHYLLGTVAGPHPFPLMVREFQRVIGREARAQILEAEG
                     RLPDACIAAVGGGSNAIGLFADFIADEDVRLIGVEAAGRGLDGGEHGATLLRGRPGIL
                     HGSETYVLQDEDGQIDESWSVSAGLDYPAVGPEHAFLKDSGRADYVGVTDAEALAAFA
                     GLARSEGIICAFESAHAMAHALKLAETAGEDMALVVNLSGRGDKDMEQARRLLAAAA"
     gene            complement(6294..7676)
                     /gene="trpCF"
                     /locus_tag="VF547_07905"
     CDS             complement(6294..7676)
                     /gene="trpCF"
                     /locus_tag="VF547_07905"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006945.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HEX8262782.1"
                     /translation="MAEGVLAEIVARKRVDVAERLADFGVEPEPTRRSLKAALARPGA
                     RFILEVKRGSPSGGAIRPGADAGALARAYRGAADAISVLTDAPYFGGSLEDLRAVRRE
                     FLGPILAKDFIVDPRQVAEARLHGADAVLVILAVLDDAEAAAVMAEARRLGMDSLVEA
                     HTAAEVRRAVALGAELIGINNRDLDTLKVDLATTERLAALVPAGTLIVAESGISTRAD
                     VARLAPHADAFLVGSSLMRAADPGEAARALAFGRVKVCGITDGHDAAMAAEHGAAYAG
                     LVMVPNTPRAVTCSAAEAVAEASPIPLVGVFRNEKPMQIAYGARALGLKAVQLHGDED
                     ALYIRALRALLPEETEIWAAAAVGREVPRPRLGADRTLFDTQVGGRSGGTGIAFDWSR
                     LEGRADLGRSVLAGGLKPANARAAARIGAFALDVGSGVEMAPGRKDAGKLHAFFEALR
                     LPARGEAACA"
     gene            complement(7669..8724)
                     /gene="trpD"
                     /locus_tag="VF547_07910"
     CDS             complement(7669..8724)
                     /gene="trpD"
                     /locus_tag="VF547_07910"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX8262783.1"
                     /translation="MSTQPNLVLATTGSEGSEAALAGLYAGESLTEGEAEGLFAELVA
                     GRLAEPAIAAMLIALRLKGETAPELIGAARALRSADSDFPRPDYLFADSCGTGGDGSA
                     SINVSTAVAFAAAAAGLPVAKHGNRSVTSRCGSADVLEQLGARLDVSAETSRRALDET
                     GVCFLFAPLYHPGLRHAGPVRRMLKVRTIMNMLGPCVNPAEPPVQLLGVADPALLEPV
                     ARTLAALGVARALVVHGSGLDEIALHGETQAVRLLDGALEEATIRPEDAGLQRRPVQV
                     LRGGGPEENAERLKALLMGYGLPAETEVVALNTGALLLTAGLAGDLREGVGMALHAIH
                     SGEADRRLRSFIEATRG"
     gene            9142..9864
                     /locus_tag="VF547_07915"
     CDS             9142..9864
                     /locus_tag="VF547_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8262784.1"
                     /translation="MRNLILGAALAYSALPLAAAAAQSQPAAIILEGTRLDVVATGEV
                     TRVPDLARISAGVVSVAPTATAALAENATRMASVRAALKRAGIADRDIQTSSINLYPD
                     YRHDPQGSNPQIIGYRASNEVSVRFRDIANSGKILDALVAQGANQINGPMLTIDKPEA
                     ALDEARTQALANARARAELYARALGKRVGRILSISEAGANYAPPVVMMRQASRGAVAD
                     STSIDPGEQSVTVSLSVSFELE"
     gene            9904..10335
                     /locus_tag="VF547_07920"
     CDS             9904..10335
                     /locus_tag="VF547_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262785.1"
                     /translation="MRLSILLLAALAAAPAAAQKLGARTATPQQVLGDMAPADPMAEA
                     ERIAAAAAAHPLGSVANPVRVGGPQGARAYIARLRCADGTRPQVGARSTGAVGAYGTL
                     TERYPLDCGAAAPGRVILAFDFYHEEHVETRPAPGFVLEGR"
     gene            10439..>11092
                     /locus_tag="VF547_07925"
     CDS             10439..>11092
                     /locus_tag="VF547_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8262786.1"
                     /translation="MRSWLRRLGTLLLFLLVGLCFAPSLVPPFLDRIYYEGPRSGHYD
                     GERFFNPDPGPRAVAHGGPARFFNRWLSGGDDRARWPEQVPVRPTVPPSRVDGAQMRV
                     TWIGHATVLIQTQGLNILTDPIWSERASPFSFVGPKRVRAPGVRFEDLPKIDLVLVSH
                     NHYDHMDLPTLNRLWERDRPLIVTSLGNDSIVGAADGVAALDWNARLPLYRRGCTATM
                     "
BASE COUNT         1584 a         3966 c         3928 g         1614 t
ORIGIN      
        1 tgccggtgag atggagcgcg atcgccccgc cgagcggcgc gagcagcagc gagcccatgt
       61 tcacgaacgg cggcacgatc cgcttgtaca ggagcaccag caccgcgaag acgaggaaga
      121 tgccggcgac caccgccatg atgaagttct cgatcagctc ctgctgcatc ttctgctcgc
      181 cctggcgggc atgctccacc ccggcgggca ggttgttgta ggagggcagc tgcttgatct
      241 tgctcatcgc ctcgaccacg cccggggcga ggtcggcgcc gacgacgatt cggcgctgct
      301 ggttgaagcg ctggatctgg gtcgggccgg cgccgaagcc gatctccgcg acgaccttca
      361 gaggcacgga gccgccgctc gtcgtcggca cgggcaggtt ctcgatggtc gagaggttct
      421 ggcgcgattc ctcgttcaga gcgacgcgga tcgggatctg ccggtcggaa agcgagaatt
      481 tggcgctgtt ctggtcgatc tcgccctggg tcgcgatccg gatcgtctgg ctgagcgcgg
      541 ccgtggtcac gcccatgtcc gcggccaggt cgaagcgcgg cttgatcacg atctcgggac
      601 gcctcaggtc gccctcgatc cgaggcgcct tcacctcggg catcttcttc atgtcctgga
      661 cgagggcgtt ggccgcctgc tccagcttca ccgggtcgtc gccggacagc atgattccga
      721 cgtcgcggtt gccgaagccc tgctgcgacc ggaaatagac ccgcgcgtcg gcgatctggc
      781 gaagcttggg cgccgtctgg cgctcgaact cgctgctggt ccgcttacgg tcgtcgctca
      841 gcgtgatgta gatgttggcg ccgccgaccc ggatcgaggc gaaggccttc accacgtccg
      901 gctccttgcg gatgatgtcg gcggcctggc tggcgattcg ctgggtctgc tgcagggtgg
      961 agcccggcgc catctcgacc gcgaccgagc tgaagtcgct gttgacgttc ggctgaaagg
     1021 tgaagggcag cgtgccgaag gcgaagatgg tggcgatgaa ggccgccacg ccgatcagca
     1081 tcgtcttcca gcggtgcttc agcgaccacc agagcaggcc catgtagcgg tccatcagcc
     1141 agccttcgcc gtggctggcg gagcccttgg cctttaggaa ataggcggcc atcaacggcg
     1201 ttatcaaccg cgcgaccagc aggctgatca gcacggcgac cacgatcgtc attccgaagc
     1261 tcttgaaata ctggccgatc agtccgccca tcatgccgac ggggaggaac accgcgacga
     1321 tcgagaaggt cgtcgccacg acggcgaggc cgatctcgtc ggccgcgtcg atcgccgcct
     1381 gataggcgct cttgcccatc cgcatgtgcc ggacgatgtt ctcgatctcg acgatcgcgt
     1441 cgtcgaccag cacgccggcg acgaggctga gcgcgagcag ggtcacgccg ttcagggtga
     1501 agcccatcag gtccatgaac cagaaggtcg gaatcgcgga aagcgggatg gcgacggccg
     1561 agatcagcgt cgcgcgccag tcgcgcagga aaaggaggac cacgagcacc gccagcacgg
     1621 cgccttcgac cagcgcctcc atggcgctgt gatactggcc ttcggtatat tggacgctgg
     1681 tgaagaggag gcggaacttg accttgccgc cgctctcctt ctcgagcttc tccatctcct
     1741 tctcggcggc gtcccagacc gtcacgtcgg aggagccctt cgccttctcg atcgagaagc
     1801 tgagcacctg gcggccgttc atcagggcga cgttgcgctg ctcggcgtag aggtcgcgga
     1861 cggtggcgat cgaggaaagc ttgatcgtcc ggccgttgcc gaccgagatg ttcttctcgc
     1921 cgaggtcgcg ggcgtcgacg gcattgccaa gcacgcgcac cgactgctcg gagccggcga
     1981 tctcggcccg gccgccggcg gcgttgacgt tggtctgccg aagctgagcg ttgacctggg
     2041 cggcggtgat gccgtgggcc tgcatcttca ccggatcgag gatgatccgg atctcgcggc
     2101 tgactccgcc gccgcgcgag acgttggcca tgccgggaat ggcgagcagg cgcttgctga
     2161 tcgtgttgtc gatgtaccag ctgagctcct ccagccccat cgccgtcgat tcgacggcca
     2221 tgtaggcgat cggcccgccg tcggcctcct cccgctggac ctgcggctcg aggattccgt
     2281 ccgggaggtc ggagcggatg ttggcgatgg cgtcgcggac gtcgttgacg gcgcggtcga
     2341 ccggagtgcc catgtcgaac tggacgaagg tcgtgctcga gccttcgcgc gcggtcgact
     2401 ggatctcgtc gacgccgttg attccccgga tcgccgcttc gacgcgctgg gtgacctgcg
     2461 tctccagctc cgacggcgcg gcgcccggct ggaccaccgt caccgtggcc gcggggaagc
     2521 tgatgtcggg gttgttgttg acgtccatgc gcaggaagct gaccagcccc agcaggctga
     2581 gcgccgcgaa cagcacgagc gggaagaccg ggttgcggat cgcccagctg gaaatgttac
     2641 gcatgtcggt ccaactccgt caggggcttc gtcagcgggc ggcgaccgcc cgttgcggcc
     2701 ggatcttctc accttcggtg aggaacggcc cggccgacag cacgactcgt tcggtcccgt
     2761 tcagcccttc ggcgatgacc acgccttcgt cggtcaccga tccgaccttc accggccggc
     2821 ggcggacggc attgtccttg tcgacgatgt agacgtagct accctggctg tcgttcagca
     2881 ccgccgattc cggaagcatc ggcgcgtcga ccgagccggc gcggatgttg gctgtggcga
     2941 agccgccggg cctgaggtcg cggtcgtagg gcacggcgat tcgcgcgacg ccctggcgcg
     3001 acgccgggtc gatgaccggc gacacctgcc agatgctccc ctgataggtc ttcgccgagc
     3061 cgaccggagt cacctgcgcg ggaatgccgg cgctgagcgt gacgaggtcc gcttcgggca
     3121 gccgcgccag catctcatac tggccgccgc gggcgattcg gaacagcgcg ccggagccgg
     3181 ggccgacgat ctggccgggc tcgacgctgc gggcgaggac caggccggcc gcgggagccg
     3241 tcacgttgag gcgtccgatg cgggctcggc tctggccgag attggcctgc gcgacccgga
     3301 cccgggcgag cgcggcgtcg cgggcggcga tccggcgctc gacgtccgcc ttggagacga
     3361 agcctcgggc gaccagcttc tgggcccggt cgagctcctg ctgggcgagg cgggcgtcgg
     3421 cgcgcgcgac gtcgatctga gcggcgagct gagccgcctc ctgggtctgc accgagcggt
     3481 cgatgacggc gagggtctgg ccggcgccga cccactgccc ggcctcgacg aggacgttga
     3541 cgaccttgcc gccctcgccg ggaatgccga ccggcatgtc gtagcgggcc gcgagctggc
     3601 cggtggcgga gatggtgcgg gcgacctgct ggcggcccgg aacgacgacg ctgaccgcgg
     3661 gcgcggcttc ggcgctctcg cccgccttgc cgccggcagc gaccttggcg tcctcgccgc
     3721 cgcggccgaa catgaggtag gcgacggcca ggaggaccag gaccgcgacg atcgcgagga
     3781 tgatgttccg gcgccggcgg cgtgccgccg tctcctcgtc gacgacgacg acgtccttct
     3841 caagggcgcc gcggcgccat ttgggcgact cgaaattcat agctctcata tttccccaca
     3901 ccggacaggc ggcagggccg cttcggctac tgtgttagcc gaatatatca cctgcctcaa
     3961 gtcaattctg ccatgcgccc cccgacgcgg cggaaccggc ctagtacatt ttttgttggg
     4021 tcttggccac tcgaaagcgg tagaaatacg aacaagcaaa tgagggcaag gagggacgca
     4081 tgaacctgct cgcggactat ggctggctcg tctggatcat cctcggcgcg atcgccggag
     4141 gcatcgccaa ggcgatcatg cccggccgcg accccggcgg ctgcatcgtc accatcctgc
     4201 tcggcatcgc cggagccgcg ctcgccggct tcatcggcaa ccagctcggg tggtacgagc
     4261 agggcgaagg cgccggcttc atcgccgcca tcgtcggagc gatcctcatc ctgttcatct
     4321 ggcggctgat cgccggacgc cgctaggcgt ctcgcgagcg cgtcgccgcc ttcatttccg
     4381 cgacgaacgg ccggacgtcc tcgccccggg cgatccgatc gacgatcgcc gagccgctga
     4441 tcacgcccgc tgcgccggcc tggagcgcga gccggacatg gtcgggccgg gcgatcccga
     4501 agcccagcac cggcggcggc gcgccggcgg ccttcagcgc cgcgatggct tcgcccgcga
     4561 agcgcacctg cgcgtcggct ccggtcaccc ccgcgcgcgc gacgcaataa gtatagccct
     4621 ccccggcctc ggcgatccgc ctcagccggt cgggcggcgt gttcggcgcg gcgatcagca
     4681 ccggcgccac ccccgccccg cgggcggccg cgaggaacgg ctcggcctcg agcatcggca
     4741 cgtcggcgac gaggacgctg tcgaccccgg ccgcggcggc ggcgcgatag aaggcctccc
     4801 gtccccgggc gacgacgata ttggcatagg tcaggatccc gaccgggacg tccgggtgcc
     4861 gttgcctgaa ttgggcgagc agcccgaagc agtccccggt ccgcgttccg gcggccagcg
     4921 cccgcaccgc cgcggcctgg atcaccgggc cgtcggcgac cgggtccgag aaggggattc
     4981 cgacctcgac catgtcggcg ccggcctcga cggcggcatc gaggatcgag gcgctcgccg
     5041 ccggatccgg atcgccgagc atggcgaagg cgccgaacgc gccctcccct cgccgccgca
     5101 gccggtcgaa catcgcggcg tagcgggtca tgccgctgcc gccagcagcc gacgcgcctg
     5161 ctccatgtcc ttgtcgccgc ggccggagag gttgacgacc agcgccatgt cctcgcccgc
     5221 cgtctcggcc agcttgagcg catgagccat cgcgtgcgcg gattcgaagg cgcagatgat
     5281 cccctcggag cgcgccagcc cggcgaaggc ggcgagcgcc tcggcgtcgg tcactccgac
     5341 atagtccgcc cggccgctgt ccttgaggaa ggcgtgctcc ggcccgaccg ccgggtagtc
     5401 gaggccggcg gataccgacc agctctcgtc gatctggccg tcctcgtcct ggagcacata
     5461 ggtttcggag ccgtggagga tcccggggcg gccgcgcagc agggtggcgc catgctcgcc
     5521 gccgtcgagc ccgcgcccgg ccgcttccac gccgatcagc cggacgtcct cgtcggcgat
     5581 gaagtcggcg aagaggccga tggcgttgga gccgccgccg accgccgcga tgcaggcgtc
     5641 gggcagccgt ccttccgcct cgagtatctg cgcccgggcc tcgcgcccga tcacgcgctg
     5701 gaactcgcgc accatcagcg ggaaggggtg cggcccggcg acggtgccga gcaggtaatg
     5761 agtctcgccg aagctggccg cccagtcccg aagcgcttcg ttgatggcgt ccttgagggt
     5821 gcgcgagccg ctctccaccg gcacgacctc ggcgcccatc agctccatcc gaaagacgtt
     5881 cgcccgctgc cgctcgacgt cgagcgcccc catgtagatc cgggtctcga ggccgaacag
     5941 cgcgccggcc agcgcggtgg cgaccccgtg ctgcccggcg ccggtctcag cgatcaaacg
     6001 gcgcttgccc atccgcttcg cgagcagcgc ctgggcgagc acctggttgg tcttgtgggc
     6061 gccgccgtga agcaggtcct cccgcttcag gtagatccgc acgcgctcgg agccgaggtt
     6121 gcggcagcgg gtcagcgggg tcggccgacc ggcgtaatgg gcgagcaggc cgtccagctc
     6181 ggcctggaag gacgggtccc cggcggcgtc gaggaaggcg gcctcgagct gctcgatcgc
     6241 cggcaccagg atctcgggga cgtaggcgcc gccataggcg ccgaagcggc cgctcaggcg
     6301 cacgccgcct ccccgcgcgc cgggagccgc agtgcctcga agaaggcgtg gagcttgccc
     6361 gcgtccttgc ggcccggcgc catctcgacg ccggagccga cgtcgagggc gaaggcgccg
     6421 atccgggccg cggcgcgggc gttggccggc ttcaggccgc ccgccagcac cgagcggccg
     6481 aggtcggccc ggccctcgag ccgcgaccag tcgaaggcga tgccggtgcc ccccgaacgg
     6541 ccgccgacct gcgtgtcgaa caaggttcga tcggcgccga gccgcggcct gggcacctcg
     6601 cggccgaccg cggcggcggc ccagatctcg gtctcctccg ggagcagcgc gcgcagcgcc
     6661 cttatgtaca gcgcatcctc gtcgccgtgg agctgcaccg ccttgaggcc gagcgcccgg
     6721 gcgccatagg cgatctgcat cggcttctcg ttgcggaaca cgccgacaag ggggatcggc
     6781 gaggcctccg cgacggcctc ggcggccgag caggtgacgg cccgaggcgt gttgggaacc
     6841 atgaccaggc cggcataggc ggcgccgtgc tcagccgcca tcgccgcgtc gtggccgtcg
     6901 gtgattccgc agaccttgac ccgcccgaag gccagcgccc gcgccgcctc gccgggatcg
     6961 gcggcgcgca tcagcgagga gccgaccagg aaggcgtcgg catggggcgc cagccgggcc
     7021 acgtcggccc gggtcgagat gcccgattcg gccacgatga gggtgccggc cgggaccagc
     7081 gccgccagcc gctcggtggt ggcgaggtcg accttcagcg tgtcgaggtc gcggttgttg
     7141 attccgatca gctcggcgcc gagcgcgacg gcgcggcgca cctccgcggc ggtatgcgcc
     7201 tcgaccagcg agtccatgcc gagccgccgg gcctccgcca tcaccgccgc cgcctcggcg
     7261 tcgtcgagca ccgcgaggat gacgaggacc gcgtccgcgc cgtgcagccg cgcctcggcg
     7321 acctggcggg ggtcgacgat gaagtccttg gcgaggatcg ggccgaggaa ctcgcgccgc
     7381 accgctcgca ggtcctcgag cgagccgccg aaatagggcg cgtcggtgag cacgctgatc
     7441 gcgtccgcgg ctccgcgata ggcccgggcg agcgcccccg catcggcgcc gggacggatc
     7501 gctccgccgg agggcgagcc gcgcttcacc tccaggatga agcgcgcgcc cggcctggcc
     7561 agcgccgcct tgaggctgcg ccgggtcggc tcgggctcga cgccgaagtc ggccaacctc
     7621 tcggccacgt cgacgcgctt gcgcgcgacg atctccgcca gcacgccctc agccacgggt
     7681 cgcctcgatg aaagatcgga ggcggcggtc ggcctcgccg gaatggatcg cgtggagcgc
     7741 catgccgacg ccctcgcgca ggtctccggc caggcccgcc gtcagcagca gggcgccggt
     7801 gttgagcgcg accacctcgg tctcggcggg caggccgtag cccatcagca gcgctttcag
     7861 tcgctcggca ttctcctcgg ggccgccgcc gcgcagcacc tgcaccggcc gccgctggag
     7921 cccggcatcc tccgggcgga tcgtcgcttc ctcgagcgcg ccgtcgagca ggcgcaccgc
     7981 ctgggtctcg ccgtgcaggg cgatctcgtc gagtccggag ccgtgcacga ccagggcgcg
     8041 cgcgactccg agggcggcca gcgtccgagc gaccggctcg agcagcgccg ggtcggcgac
     8101 tccgagcagt tgcacgggcg gctcggccgg gttcacgcag gggccgagca tgttcatgat
     8161 cgtgcgcacc ttgagcatcc gccgcaccgg cccggcgtgg cgcaggccgg gatggtagag
     8221 cggggcgaac aggaagcaga ccccggtctc gtcgagcgcc cggcgcgacg tctcggcgga
     8281 cacgtccagc cttgccccga gctgctcgag cacgtcggcc gagccgcagc gcgaggtcac
     8341 cgagcggttg ccgtgcttgg cgacgggaag gccggcggcg gcggcggcga aggcgacggc
     8401 ggtcgagacg ttgatcgacg ccgagccgtc gccgccggtg ccgcagctgt cggcgaacag
     8461 gtagtcgggc cgggggaagt cggaatcggc cgagcgcagc gcgcgggcgg cgccgatcag
     8521 ctccggcgcg gtctcgccct tgaggcgaag ggcgatcagc atggcggcga tggcgggctc
     8581 ggccagccgc ccggcgacga gctcggcgaa caggccctcg gcttcgcctt cggtcaggga
     8641 ttcgccggcg tagagaccgg ccagcgcggc ttcggagcct tcggatccgg tggtggcgag
     8701 gacgaggttg ggctgggtgg acatggcgtg attccttgga tggagcgcaa aagaaaaggc
     8761 ccgccgggag cggcgggcct ctgctgggac ttcggacctt tctagagagt ccggttcacc
     8821 atcgatggac gcgcgccgcg gcggaggaga tgtcccacca ccaatgccag gcgtgcgcgg
     8881 cggccggcgc cgggaaggcg cggaaggagc cggtgcgggt tgcctgggtc gtcatcgcct
     8941 cctcaactcc cagctaaagc cctgcaagtc aatcctaatc tcaccgtctt cccagcgaag
     9001 gccggacccc aggcggcgaa ggcgccgaag tccttcaccc gtcggggccc cagcctttcg
     9061 ccgggggcga cgatgttcag caacggtgca gccgtcgcgg cgttgaaagg accggaagcg
     9121 aaaccttctt ggagtatctc gatgcgtaac ctgatcctcg gcgccgccct cgcctactcg
     9181 gcgctgccgc tcgccgccgc cgccgcccag agccagccgg cggcgatcat cctcgaaggc
     9241 acccgcctcg acgtcgtcgc caccggcgag gtcacccggg tccccgacct cgcccggatc
     9301 agcgccgggg tcgtctcggt cgctccgacc gcgaccgccg cgctcgccga gaacgccacc
     9361 cggatggcgt cggtgcgggc ggcgctgaag cgggccggga tcgccgaccg cgacatccag
     9421 accagctcga tcaacctcta tcccgactat cgccacgacc cgcagggcag caatccgcag
     9481 atcatcggct accgcgccag caacgaggtc agcgtccgct tccgcgacat cgccaacagc
     9541 ggcaagatcc tcgacgcgct cgtcgcgcag ggcgcgaacc agatcaacgg gccgatgctg
     9601 accatcgaca agcccgaggc ggcgctcgac gaggcgcgga cccaggcgct cgccaacgcc
     9661 cgcgcgcgcg ccgagctcta cgcccgggcg ctcggcaagc gggtcggccg gatcctctcg
     9721 atcagcgagg ccggcgccaa ttacgcgccg ccggtggtga tgatgcgcca ggcgtcgcgc
     9781 ggcgccgtcg ccgattcgac ctcgatcgat cccggcgagc agagcgtgac ggtcagcctg
     9841 tcggtgtcgt tcgagctgga atgacggttt ccttttccgg gcgaacgcct tagcgcgcgg
     9901 cgaatgcgac tctcgatcct tctcctcgcc gccctcgccg ccgcgcccgc cgcggcccag
     9961 aagctcggcg cgcggaccgc gacgccccag caggtgctcg gcgacatggc gccggccgat
    10021 ccgatggcgg aagccgagcg gatcgccgcg gccgcggccg cccacccgct cggcagcgtc
    10081 gccaatcccg tccgggtcgg cggcccgcag ggcgcccgcg cctatatcgc ccggctgcgc
    10141 tgcgccgacg gcacccgccc gcaggtcggc gcgcgcagca ccggcgcggt cggcgcctac
    10201 ggcacgctga ccgagcgcta cccgctcgat tgcggcgccg ccgcccccgg ccgggtcatc
    10261 ctggccttcg atttctacca cgaggagcat gtcgagaccc ggccggctcc cggcttcgtc
    10321 ctcgaggggc gctgatcgat cccccgccaa ggccggccgg cggagctgcg gcaggaccgg
    10381 gtcccgacct tcgccgggac gcatgacttt cggcctcgac aggccctagg acggagggat
    10441 gcgatcctgg ctgcgccgtc tcggcaccct cctcctcttc ctgctcgtcg gcctgtgctt
    10501 cgctccgtcg ctggtcccgc ccttcctcga ccggatctat tacgaaggcc cgcggtccgg
    10561 tcattatgac ggcgaacgct tcttcaaccc cgatcccggg ccccgagcgg tcgcgcacgg
    10621 cggcccggcg cgcttcttca accgctggct gagcggcggc gacgaccgcg cccgctggcc
    10681 ggagcaggtg ccggtccggc cgacggtgcc gccgagccgg gtcgacggcg cgcagatgcg
    10741 ggtcacctgg atcggccacg ccaccgtcct catccagacc caggggctca acatcctgac
    10801 cgatccgatc tggtccgagc gcgcctcgcc tttctccttc gtcggcccga agcgggtgcg
    10861 cgcgccgggg gtgcgcttcg aggacctgcc gaagatcgac ctcgtcctcg tcagccacaa
    10921 ccattacgac catatggacc tgccgaccct caaccggctc tgggaacggg accggccgct
    10981 gatcgtgacc agcctcggca acgattcgat cgtcggcgcg gcggacggcg tggccgccct
    11041 cgactggaac gcgcgcctgc cgctctaccg ccggggctgc acggcgacga tg
//
LOCUS       DASVQZ010000290         8875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_350560, whole genome shotgun sequence.
ACCESSION   DASVQZ010000290 DASVQZ010000000
VERSION     DASVQZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350560"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VF547_07930"
     CDS             complement(<1..747)
                     /locus_tag="VF547_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262787.1"
                     /translation="MTLSKIVPFRPPGPTRSNSIRTSQYQTKLDRQAGFRRSLVKRLE
                     DALFADQGHTSVGERVPFVPAQMTFTCCTGVIKYFYCREKKSYLLTDFSLGDGGNGGQ
                     PISVDPDESSYHVAAAVGWARNLAARWAFSVIRLVAHAMLKARLLSVPPLFPRPMTEW
                     LTRVRSANTAILREIAVLGRVMNSAQSDICPDALPSWSILRHSVTTAQQLDNYYARSI
                     LAGEALTRLGAEVDLGLIVTRCQYLAEQDED"
     gene            complement(749..1387)
                     /locus_tag="VF547_07935"
     CDS             complement(749..1387)
                     /locus_tag="VF547_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262788.1"
                     /translation="MFSLRNVPSYDQRSLALIEAFLLGDSSGGIRLRPREEIERAMES
                     GLCLEISRDGAICGCSLIYKFDTWPGGPVYAEMGSMLVTARPYSLQWFLAAFHLFQIY
                     LEEFDSNPSEIFAVVAARSASEHNLRDKAGLVGMAPPAALQVIRAGRGLAFAPDKPVL
                     FAKAESFDRAFADLRSWHEKGRVFRTPKGGERIEIEVEWFLPELLDLLGGGK"
     gene            1553..1879
                     /locus_tag="VF547_07940"
     CDS             1553..1879
                     /locus_tag="VF547_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262789.1"
                     /translation="MEIEMSATRPFTDLMVHSQISLLRSLHGALEHKRVRTRDEFIDL
                     MKAAFEHGKLDPRRLADDLGYSFSTVYRWIDGRSAPHPSLWPRIVEWTMGAIVARIEE
                     CEGIGR"
     gene            1951..3564
                     /locus_tag="VF547_07945"
     CDS             1951..3564
                     /locus_tag="VF547_07945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8262790.1"
                     /translation="MTLDLHRRLLLKAGTLGLGALAVPGVAQIAASRGFGHGVASGEP
                     RARSVMLWTRHVPGGRLHWQVSRSADFARIAAEGDVVAEAEHDFCVKPVATGLQPGSW
                     YHYRFRDARGAVSPVGRTRTLPEGRTRRFALGVFSCANLGFGWFNAYAHAAGRRDLDL
                     LVHLGDYFYEYERGKYPSARESLAGRELDPATEAVALADYRLRHAAYRSDPDLQRLHA
                     SAPMVMMWDDHETANDSWSGGAENHDPAKEGPWAARKAAAQRAYREWLPVSDNSWESY
                     EIGDLATLFRPETRLTARSRPLVYGEAVRRGEDYKVSLMRFRDGPWRDPDRTMLGSEQ
                     EAWLAGAFRRSTSQGTRWQLLAQQTVMGSWALPVEARAWMRSDAPEAVRRITTIGAAA
                     SEIGLPLNLDAWDGYPAARERLLRSALDSNSNLIVLSGDSHNGWAFDLDLAGTPAGAE
                     FAGASVTSPGLEAYAPGVAPAEVERAVRARNPALKWVELRRRGYLALTLTPDRATGEW
                     LSLETVRTRSTRLAGRNRMSVTRGSNRFT"
     gene            complement(3626..3913)
                     /locus_tag="VF547_07950"
     CDS             complement(3626..3913)
                     /locus_tag="VF547_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262791.1"
                     /translation="MRRCEVDGLDPARTWWVPAVVAPRRDWAGAPGCRRGARFVVDPE
                     TMHATRDAFTPFDSRAGCLDWIMRHRAELNRTLPGTTVRAVMLDRWLLGLE"
     gene            complement(4081..6309)
                     /gene="katG"
                     /locus_tag="VF547_07955"
     CDS             complement(4081..6309)
                     /gene="katG"
                     /locus_tag="VF547_07955"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX8262792.1"
                     /translation="MDAKTSGSPLSDPNKEPAALRSLLGRTNRDWWPNQISLDILHQN
                     GAASPSPFGDDFDYPREFESLDYQALKRDLTALMTDSQPWWPADYGHYGPFFIRMAWH
                     SAGTYRTGDGRGGASSGGQRFAPLNSWPDNANLDKARRLLWPIKQKYGRKLSWADLMI
                     LAGNVAIESMGGPTFGFGGGRVDVWEPQKDIYWGTEEEWLGETRIDEQSGKALENPLA
                     AIQMGLIYVNPQGPGGNPDPLQSARDIRETFSRMAMNDEETVALTAGGHTFGKCHGAG
                     DASKVGAEPEGADIAQQGLGWTSGHESGIGDHTITSGLEGAWTPTPIQWSMNYFRMLL
                     EYEYELVRSPAGALQWQPVDQKPEDMAPGAHSPDRRFPTMMTTADMAFKMDPKYREIS
                     ERFYRNPDQFADAFARAWFKLTHRDMGPKVRYLGPEVPDEDLIWQDPVPPGQKLSDAD
                     VASLRGKIADSSLTVAQLVKTAWASAATFRGSDKRGGANGARIRLEPQRSWEVNEPEE
                     LGPVLQVYEDIRAGFGGRVSLADLIVLGGCVGVEQAARAAGHAVTVPFAPGRGDASQE
                     QTDVESFSVLEPKADGFRNYLQVRFSVPTEELLIDRAQLMQLSAPEMTVLVGGLRVLG
                     ANHGGSKHGVFTDRPGQLTNDFFVNLLDMGTAWRQVDDRGDEVFLGTCRLTDDEKWTA
                     TRTDLVFGSNSQLRAIAEVYACDDAKEKFVEDFVAAWTKVMNADRFDLVDPSCHAPEE
                     AA"
     gene            6497..8815
                     /locus_tag="VF547_07960"
     CDS             6497..8815
                     /locus_tag="VF547_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX8262793.1"
                     /translation="MLKKTSAPDLEALRLIDDRLRWLSAWTIHHANHLRESPDGLKVG
                     GHQASCASMTAIMAALYFHALGPNDRIAVKPHAGPVLHAVHYLLGSQSLEQLENFRGF
                     KGMQSYPSRTKDRIPVDFSTGSVGLGVAITAFASLVQDYLIAHGEMAPSDAGRMVALM
                     GDAELDEGNIYECLIEAYKHDIRNCWWIVDYNRQSLDATTAERMFSRFDEIFASCGWR
                     VVTLKYGKAMEAAFREPGGEALKRWIDEAGNADYAALTYLGGAAWRERLAKEAPEARP
                     VLDARDDEALAALMTNLAGHDMASLVEAFDAAQDDVPTLFIAYTIKGFGLPFAGHKDN
                     HSGLMNPGQIAALRDKMGISEGEEWAPWAGLGGNAAEAVRNVAENSRIARAKRERPWN
                     QAEVPAIPVPTGDEQSTQAAFGRILLDLSRAGGSLADRIVTTSPDVTVSTNLGAWVNQ
                     RGLFRRQALADVFAAAKIPSAQKWGGHDAGQHIELGIAENNLFLMLAALGLAGDLFGE
                     RLMPIGTVYDPFIARGLDALNYACYQDARFMLVATPSGVTLGPEGGAHQSINPPLIAL
                     GQPGLRHYEPAFVDELALLMEEGFRLMQAPDGESMYLRLTTRTVSQVAREDDSWKAGA
                     IAGGYWLREPAPGAEAALVYSGAIAPEALAAWEALKDDLPGLGLLAVTSPDLLHRGWS
                     ERRRGRWGGRGTALSHVDQLLAPLSPSAGLVTLLDGAPASLSWLGGVMGHKVSPLGIE
                     RFGQTGTLNDLYREYRLDPDAIVEAAAELFLD"
BASE COUNT         1398 a         3080 c         2971 g         1426 t
ORIGIN      
        1 atcctcgtcc tgttcggcaa gatattggca gcgggtgacg atcagtccca ggtcgacctc
       61 cgcgccgagg cgggtcaggg cttcgccggc gaggatcgag cgcgcgtagt aattatccag
      121 ttgctgcgca gtcgtcaccg agtgacgaag aatcgaccag gagggcaggg cgtccggaca
      181 gatatcgctc tgcgctgaat tcatgacccg cccgagcacg gctatctccc gcaggatggc
      241 cgtgttggcg gatcttaccc gagtgagcca ctcggtcatg ggacgcggga aaagcggcgg
      301 caccgaaagc agccgcgcct tcagcatggc gtgggcgaca agtcgaatca ccgaaaaggc
      361 ccagcgggcc gcgaggttcc gggcccagcc gaccgcggcg gcgacatgat aggaggattc
      421 gtcggggtcg acggagatcg gctgcccccc gttgccgccg tctccaagcg aaaaatcggt
      481 cagaaggtag ctctttttct ccctgcaata gaaatacttg atcacgccgg tgcagcaggt
      541 gaacgtcatc tgcgccggca cgaaaggaac ccgttctccg acgctggtgt ggccctgatc
      601 cgcgaacaag gcgtcctcga ggcgcttgac caggctgcgg cggaaccccg cctggcggtc
      661 cagcttcgtt tgatattgcg aggttctgat cgagttcgac cgcgtcggcc cgggcggacg
      721 aaatggaact atcttgctga gggtcatatc attttccgcc gccaaggagg tccagaagct
      781 ccggcaggaa ccactcgacc tctatttcga tacgttcgcc tccctttggc gtcctgaaga
      841 cgcggccctt ctcgtgccag gacctgaggt cggcaaacgc gcgatcgaag ctctcggcct
      901 tggcgaagag gaccggcttg tccggtgcga aggccaggcc ccgtcccgcc ctgatgactt
      961 gaagcgccgc cggcggggcc atgccgacga gtcccgcctt gtcgcgaaga ttatgctcgg
     1021 acgcggatcg cgcggccacg accgcgaaga tctccgacgg attggagtcg aactcctcga
     1081 ggtagatctg gaacagatga aacgcggcca ggaaccattg cagggagtag ggccgcgcgg
     1141 tgacgagcat gctccccatt tccgcgtaga cggggccgcc gggccaggtg tcgaacttgt
     1201 agatcaggct gcaaccgcaa atggcgccgt cccggctgat ctccaggcag agcccggact
     1261 ccatcgcacg ctcgatctcc tcgcgcggtc gcaggcgaat gccgccgctc gagtcgccga
     1321 gcaggaacgc ctcgatcagg gcgaggctgc gctggtcgta cgaggggacg ttgcgaaggc
     1381 tgaacatgga gaccgcaatc cctgacgtcc aaaggcgccc ccacactcca catggcataa
     1441 tgcgacaaaa tttgcaagtt gcaaatatct tgcaatttgc tataatttaa ctacttgcga
     1501 gcaggaaacc tgtctcgcgt gggatcgagt cgctcgtacc gggcagaaag gcttggagat
     1561 cgaaatgtcc gccacccgac cctttaccga cctcatggtt cacagccaga tcagcctatt
     1621 gcgcagcctc cacggtgcgc tcgagcataa gcgcgttcgg actcgcgacg aattcatcga
     1681 cctcatgaaa gcggccttcg agcacggcaa gcttgaccca cggcgactgg cggacgactt
     1741 ggggtacagc ttttccacag tgtatcgctg gatcgacggc cgtagcgctc cgcaccccag
     1801 cctgtggccg cggattgtgg agtggacgat gggcgcgatc gtcgccagga tcgaggaatg
     1861 tgaagggatc ggtcgctagc ccgacttccc ttcctatggc ggacgaggcg gcggttgcct
     1921 tgcccgcgtt cactctccac aacctggcgc atgaccctcg acctccaccg ccgcctcctc
     1981 ctcaaagcgg gaacgctcgg cctcggcgcg ctcgcggtgc cgggggtggc gcagatcgcc
     2041 gcctcgcgcg gcttcggcca cggcgtcgcc agcggcgagc cgcgagcgcg ctcggtgatg
     2101 ctctggaccc gtcacgtccc cggcggccgg ctgcactggc aggtgtcgcg ctcggccgac
     2161 ttcgcccgga tcgccgcgga gggggacgtc gtcgcggagg cggagcatga tttctgcgtg
     2221 aagccggtcg cgaccggcct ccagcccggc agctggtacc attaccgctt ccgcgacgcc
     2281 cgcggcgccg tctcgccggt cggccggacc cggaccctcc cggagggccg cacccgccgc
     2341 ttcgcgctgg gcgtcttctc ctgcgccaat ctcggcttcg gctggttcaa cgcctacgcc
     2401 cacgccgccg gccgccgcga cctcgacctg ctcgtccacc tcggcgacta tttctacgaa
     2461 tatgagcgcg gcaaatatcc gtccgcgcgc gagagcctcg ccgggcgcga gctcgatccc
     2521 gcgaccgagg cggtggcgct cgccgattac cgcctgcgcc acgccgccta tcgctcggac
     2581 cccgacctgc agcggctcca tgccagcgcg ccgatggtga tgatgtggga cgatcatgag
     2641 accgccaacg acagctggtc gggcggcgcc gagaatcacg atccggcgaa ggaggggccg
     2701 tgggcggcgc gcaaggcggc ggcgcagcgc gcctatcggg aatggctgcc ggtttccgac
     2761 aacagctggg agagctacga gatcggcgac ctcgccaccc tgttccggcc ggaaacccgg
     2821 ctcaccgccc gcagccggcc gctggtctac ggcgaagcgg tccggcgcgg cgaggactac
     2881 aaggtctcgc tgatgcgctt ccgcgacggc ccctggcgag accccgaccg gacgatgctg
     2941 ggctcggagc aggaggcctg gctggccggc gcgttccggc gctcgacctc tcaggggacc
     3001 cgctggcagc tgctcgccca gcagacggtg atgggctcct gggcgctgcc ggtggaagcg
     3061 cgcgcctgga tgcgctccga cgcgcccgag gccgtccgcc ggatcaccac catcggcgcc
     3121 gcggcatccg agatcggcct gcctctcaac ctcgacgcct gggacggcta tccggccgcc
     3181 cgcgagcgcc tgctccggtc cgccctcgac tccaactcca atctcatcgt cctgtcgggc
     3241 gacagccaca acggctgggc gttcgacctc gacctcgccg gcactccggc gggcgccgag
     3301 ttcgccgggg ccagcgtcac ctcgcccggc ctggaggcct atgcgccggg agtcgccccc
     3361 gccgaggtcg agcgcgccgt ccgcgcccgc aacccggcgc tcaaatgggt cgagctgcgg
     3421 cggcgcggct atctggcgct gaccctgacg cccgaccgcg ccaccggcga atggctgtcg
     3481 ctggaaacgg tccggacccg ctcgacgcgg cttgcggggc gcaacaggat gagcgtgacc
     3541 agaggttcga accgcttcac ctgacccaat ctcccgatgc tggggatacc tccccacggc
     3601 tccgccgcag ggaggatcca ggcgctcact ccagtcccag gagccagcgg tccagcatca
     3661 cggcccgcac cgtcgttccg ggcagcgtcc ggttgagctc ggcgcgatgg cgcatgatcc
     3721 agtcgaggca gccggcccgg ctgtcgaacg gggtgaaggc gtcgcgcgtg gcgtgcatcg
     3781 tctcggggtc gacgacgaac ctcgcccctc gacggcatcc gggcgcgccg gcccagtccc
     3841 ggcgaggggc gacgacggcc ggcacccacc aggtccgggc cggatccagg ccgtcaacct
     3901 cgcaccggcg catcgccttc actcccctgg cctggagcct cgaccgaccg atcctcccag
     3961 cctccgttcg ggctgagcct gtcgaagccc ttctcttacc ctttgggaac aagaaggaag
     4021 gcccttcgac aggctcaggg cgaacggact ggggacgact caatttcaaa ctctcgacgt
     4081 tcaggcggcc tcctcgggcg cgtggcagct cgggtcgacg aggtcgaagc ggtcggcgtt
     4141 catcaccttg gtccaggccg cgacgaaatc ctcgacgaac ttctccttcg cgtcgtcgca
     4201 agcatagacc tcggcgatcg cccgcagctg cgagttggag ccgaagacga ggtcggtccg
     4261 ggtcgccgtc cacttctcgt cgtcggtgag gcggcaggtg ccgaggaaaa cctcgtcgcc
     4321 gcggtcgtcc acctgccgcc acgccgtgcc catgtcgagc aggttgacga agaagtcgtt
     4381 ggtgagctgg cccggccggt cggtgaacac gccgtgcttc gagccgccgt gattggcgcc
     4441 gagcactcgg aggcctccga ccagcaccgt catctccggc gcgctgagct gcatcagctg
     4501 cgcccggtcg atcagcagct cctcggtggg gacgctgaac cggacctgga gataattgcg
     4561 gaagccgtcc gccttgggct ccagcaccga gaagctctcg acgtcggtct gctcctggct
     4621 ggcatcgccg cggcccgggg cgaaaggcac ggtcacggcg tggcccgcgg cccgggccgc
     4681 ctgctcgact ccgacgcagc cgccgagcac gatcaggtcg gcgaggctga cccggccgcc
     4741 gaagccggcg cggatatcct catagacctg gaggaccgga ccgagctcct cgggctcgtt
     4801 gacctcccag ctgcgctgcg gctccaggcg aatccgggcg ccgttggcgc cgccgcgctt
     4861 gtcggagccg cggaaggtcg cggccgacgc ccaggccgtc ttgaccagct gggcgacggt
     4921 gaggctggaa tcggcgatct tgccgcgcag gctcgcgacg tccgcatcgc tgagcttctg
     4981 ccccggcggc accgggtcct gccagatcag atcctcgtcc gggacctccg ggccgaggta
     5041 gcggaccttg ggccccatgt cgcggtgggt cagcttgaac caggcgcggg cgaaggcgtc
     5101 ggcgaactgg tccgggttgc ggtagaagcg ctcggaaatc tcgcgatatt tggggtccat
     5161 cttgaacgcc atgtcggcgg tcgtcatcat cgtcggaaac cgccggtcgg ggctgtgcgc
     5221 gccgggcgcc atgtcctcgg gcttctggtc gaccggctgc cattgcagtg ctccggccgg
     5281 gctcctcacc agctcatatt catattcgag cagcatccgg aaatagttca tcgaccactg
     5341 gatcggagtc ggggtccagg cgccctcgag gccgctggtg atggtatggt cgccgatgcc
     5401 gctctcgtgg ccggaggtcc agccgagccc ctgctgggcg atgtcggcgc cttccggctc
     5461 ggcccccacc ttggaggcgt ccccggctcc gtggcacttg ccgaaggtgt ggccgccggc
     5521 agtcagggcg accgtctcct cgtcgttcat cgccattcgc gagaaggtct cgcggatgtc
     5581 gcgggccgat tgcagcggat cgggattgcc gcccgggccc tggggattga cgtagatgag
     5641 gcccatctgg atcgcggcga gcgggttctc aagcgccttg ccggactgct cgtcgatgcg
     5701 ggtctcgccc agccactcct cctcggtgcc ccagtagatg tccttctgcg gctcccagac
     5761 gtcgacgcgg ccgccgccga agccgaacgt gggtccgccc atcgattcga tggcgacgtt
     5821 gccggccagg atcatcagat cggcccagct cagcttgcgg ccgtatttct gcttgatcgg
     5881 ccagagcagc cggcgcgcct tgtcgagatt ggcgttgtcg ggccaggagt tgagcggcgc
     5941 gaagcgctgc ccgcccgacg aggcgccgcc gcggccgtcg ccggtccggt aggtgccggc
     6001 gctgtgccag gccatgcgga tgaagaaggg tccgtaatgc ccgtagtcgg ccggccacca
     6061 gggctggctg tcggtcatca gagcggtgag gtcgcgcttg agagcctggt agtccagcga
     6121 ctcgaattcg cggggatagt cgaagtcgtc gccgaacggg ctcggcgaag cggcgccgtt
     6181 ctggtggagg atgtcgagcg agatctggtt cggccaccag tcgcgattgg tccgcccgag
     6241 cagggaccgg agcgcggcgg gctccttgtt cgggtcgctg agggggctgc cgctggtctt
     6301 tgcgtccatt tcgtcctctc cggttcttgt cgcaccaggt cgcgccgccg gggaggcggc
     6361 aggaagccgc cctgctaggc cgggcaaacg cagcgcgcaa acggctaaac ccggcttgtc
     6421 tgatcggttc ggcaaatgac gcagcgcagc agactgaccg ctgtcccgtt cgccccgagg
     6481 gcgctaaggg cacgccatgc tgaagaagac ctccgcgccc gacctcgagg cgctgcgcct
     6541 gatcgacgac cggcttcgct ggctcagcgc ctggacgatc catcacgcca accacctgcg
     6601 cgagagcccg gacggcctca aggtcggcgg gcaccaggcg agctgcgcct caatgacggc
     6661 gatcatggcg gcgctctact tccacgcgct cggcccgaac gaccggatcg cggtgaagcc
     6721 gcatgccggg ccggtgctcc acgccgtcca ttatctgctc ggctcgcaga gcctcgagca
     6781 gctcgagaat ttccgcggct tcaagggcat gcaatcctat ccgagccgca ccaaggaccg
     6841 gatcccggtc gacttctcga ccggctcggt cggcctcggc gtcgcgatca ccgccttcgc
     6901 cagcctcgtc caggactatc tgatcgccca tggcgagatg gcgccgtcgg acgcggggcg
     6961 gatggtcgcc ctgatgggcg atgccgagct cgacgagggc aatatctacg aatgcctgat
     7021 cgaggcctac aagcacgaca ttcgcaattg ctggtggatc gtcgactata atcgccagtc
     7081 gctcgacgcg accaccgcgg agcgcatgtt cagccgcttt gacgagatat tcgcgagctg
     7141 cggatggcgc gtcgtcacgc tgaaatacgg caaggcgatg gaggcggcgt tccgcgagcc
     7201 gggcggcgag gcgctgaagc gctggatcga tgaggcgggc aatgccgatt acgccgcgct
     7261 cacctatctc ggcggcgccg catggcggga gcggctggcg aaggaagcgc ccgaagccag
     7321 gcctgtgctc gacgcgcgcg acgacgaggc gctggccgcg ctcatgacca acctcgccgg
     7381 ccacgacatg gcgtcgctcg tcgaggcgtt cgacgcggcc caggacgacg tgccgaccct
     7441 gttcatcgcc tacacgatca aaggcttcgg cctgccgttc gccggccaca aggacaatca
     7501 ttcggggttg atgaaccccg gccagatcgc cgccctgcgc gacaagatgg gaatatcgga
     7561 aggggaggag tgggcgccct gggccggcct cggcggcaat gcggcggaag ccgtgcgcaa
     7621 cgtcgccgag aacagccgca tcgcccgggc gaagcgggag cggccgtgga accaggccga
     7681 ggtgccggcc atccccgtgc caaccggcga cgagcagtca acccaggccg cgttcggccg
     7741 catcctcctt gacctcagcc gcgccggcgg cagccttgcc gaccggatcg tcaccacctc
     7801 gccggacgtg acggtctcga ccaacctcgg cgcgtgggtg aaccagcgcg gcctgttccg
     7861 ccgacaggcg ctggcggacg tcttcgccgc cgccaaaatc ccgtcggcgc agaaatgggg
     7921 cgggcacgac gccgggcagc acatcgagct cggcattgcc gagaacaacc tcttcctgat
     7981 gctcgccgcg ctcggcctcg ccggcgacct gttcggcgag cggctgatgc cgatcggcac
     8041 cgtctacgat cccttcatcg cccgcggcct cgatgcgttg aactatgcct gctaccagga
     8101 tgcgcgcttc atgctcgtcg cgacgccctc gggcgtcacg ctcgggcccg agggcggcgc
     8161 ccaccagtcg atcaacccgc ctctgatcgc gctcggccag cccggcctca ggcattacga
     8221 gcccgccttc gtcgacgaac tggcgttgct catggaggaa ggctttcggc ttatgcaggc
     8281 gcccgacggg gagagcatgt atctccgcct caccacccgc accgtcagcc aggtcgcccg
     8341 cgaggacgac agctggaaag cgggcgccat tgccggtggc tactggctgc gcgaacccgc
     8401 tccgggcgcc gaagccgctt tggtctattc cggcgcgatc gctcccgaag ccctcgcggc
     8461 ctgggaggcg ctgaaggacg acttgcccgg ccttggcctc ctcgccgtga cgtcgccgga
     8521 cctcctccac cgcggctgga gcgagcgccg ccgcggccgc tggggcggca ggggcacggc
     8581 gctcagccat gtggaccagc tcctcgcgcc gctctcccca tctgcaggcc tggtcaccct
     8641 cctcgacggc gcgcccgctt cgttgtcctg gcttgggggc gtgatgggcc acaaggtcag
     8701 cccgctcggc atcgagcgct tcggccagac cggcacgctt aatgacctct accgcgaata
     8761 ccgcctcgac cccgacgcca ttgtggaggc cgccgccgag ctgttcctcg actaggcccg
     8821 atgcttgtcc gacgctgagt cgtcatgccg gacttgatcc ggcatccacc ttctc
//
LOCUS       DASVQZ010000291         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_540898, whole genome shotgun sequence.
ACCESSION   DASVQZ010000291 DASVQZ010000000
VERSION     DASVQZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540898"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VF547_07965"
     CDS             <1..549
                     /locus_tag="VF547_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8262794.1"
                     /translation="LQAGTQRRGDAARGLPIVVLVDSGSASAAEIVAAALQDHHRALV
                     MGERSFGKGSVQTLVPLTQKTALRLTTARYYTPSGRSVQEGGITPDIEVPQLSDPDYK
                     SRPKLREADLRRHLINEAKVDNDVIQDDGKPDPRFAATAEQLKAKGIEDFQLHYALQT
                     ISRLGRNAAATPAGGARPTGRR"
     gene            626..1114
                     /locus_tag="VF547_07970"
     CDS             626..1114
                     /locus_tag="VF547_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX8262795.1"
                     /translation="MTSLDKARALAIAVPAALLAGAYGSEYWGGLHPCEMCWWQRYAH
                     FVGLAFALLSLALRSFPDRGRSLVWLAALAILASGAIGAYHAGVEAGIFEGFTQCTSN
                     MAGLSGDDLLKAVMAAPVVRCDQIQFEFLGISMAGWNAILSIAAALTILWLSLRRPRT
                     RR"
     gene            1183..1671
                     /locus_tag="VF547_07975"
     CDS             1183..1671
                     /locus_tag="VF547_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEX8262796.1"
                     /translation="MVRVDQAGEYGATRIYCGQLAVLGSHPASRSIARMQSQEEGHLR
                     TFDEMMVERGVRPTLLQPVWRVAGHALGVATALMSPSAAMACTAAVETEIDRHYADQL
                     RALGAGEPDLAATIGRFREEELEHRDTALAAGAENALGYPLLSSLIRAGCRIAIALSK
                     RI"
     gene            1676..2167
                     /locus_tag="VF547_07980"
     CDS             1676..2167
                     /locus_tag="VF547_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262797.1"
                     /translation="MKLLAAAAVAALLASAGPGHAQAPAAEPKVSAMIVYGDDPCPPS
                     TDDTIVVCARKPEGERYRIPEVLRGDPNDPKNQAWAERATALEYAGRGGIGSCSTVGP
                     GGASGCFNQLVREARAAGRTADEVNWNALIEQARRERLGKIDEQAEAEEADAVADEGG
                     TPR"
     gene            2257..>2447
                     /locus_tag="VF547_07985"
     CDS             2257..>2447
                     /locus_tag="VF547_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262798.1"
                     /translation="MKAALSIAALAAAGVAAVSVAPAPAEAQGAQQERIRRLVVYGRD
                     PCPRAAAGEDIVICARRPET"
BASE COUNT          374 a          919 c          820 g          334 t
ORIGIN      
        1 ctccaggctg ggacgcaacg ccgcggcgac gccgcccgcg gcctgccgat cgtggtcctc
       61 gtcgattccg gcagcgcgtc ggcggcggag atcgtcgcgg ccgccctcca ggaccaccac
      121 cgcgccctgg tcatgggcga gcgcagcttc ggcaagggct cggtccagac cctggtcccg
      181 ctgacccaga agacggcgct tcggctgacc acggcgcgct actacacgcc ctcgggccgg
      241 tcggtgcagg aaggcggaat caccccggac atcgaggtcc cgcagctctc cgacccggac
      301 tacaagtcgc ggcccaagct ccgcgaggcc gatctcaggc gccacctgat caacgaggcg
      361 aaggtcgaca acgacgtcat ccaggacgac ggcaagcccg atccgcgctt cgccgccacc
      421 gccgagcagc tcaaggccaa ggggatcgag gacttccagc tccattacgc gctgcagacc
      481 atctccaggc tgggacgcaa cgccgcggcg acgccggcgg gcggggcgcg tccgaccgga
      541 aggcgctgat gcagcttcgt catgccagcg aaggctggca tccatgccgg gtcagcatgg
      601 acctcagctt tcgctggggt gacgaatgac ctcgctcgac aaggcccgcg ccctcgcgat
      661 cgcggtcccg gccgccctgc tcgccggcgc ctacgggtcc gaatattggg gcggcctgca
      721 cccctgcgag atgtgctggt ggcagcgcta cgcccatttc gtcggcctcg ccttcgccct
      781 gctctcgctc gccctgcgca gcttcccgga ccgcggccga agcctggtct ggctcgccgc
      841 cctcgccatc ctggcgagcg gggcgatcgg cgcctatcat gccggggtcg aggcggggat
      901 cttcgaaggc ttcacccaat gcacgtcgaa catggccggg ctctcgggcg acgatctgct
      961 caaggcggtg atggcggcgc cggtggtgcg ctgcgaccag atccagttcg agttcctcgg
     1021 aatctcgatg gcgggatgga atgcgatcct ttcgatcgct gccgcgttga cgatattgtg
     1081 gctgagcctc agacgacccc ggactcggcg gtgagcgccg ccccaagccc gccgaaggcg
     1141 ccgcgctggc gtcccggcga cgagaagccc gacatcgcct cgatggtccg ggtcgaccag
     1201 gccggcgaat atggcgccac ccgaatctat tgcggccagc tcgccgtgct cgggtcgcac
     1261 ccggcgagcc ggtcgatcgc ccgaatgcaa tcccaggagg aaggccacct ccgcaccttc
     1321 gacgagatga tggtcgagcg cggagtgcgg ccgaccctgc tccagccggt ctggcgggtg
     1381 gccggacatg ccctgggagt cgccaccgcc ctgatgagcc cgagcgcggc catggcctgc
     1441 accgcggccg tcgagacgga gatcgaccgc cattatgccg accagctgcg cgcgctcggc
     1501 gccggcgagc ccgacctcgc cgccaccatc ggccgcttcc gcgaggagga gctcgagcat
     1561 cgcgacaccg ccctagcggc cggcgcggag aatgcgctcg gctatccctt attgtcttcc
     1621 ctcattcgcg ccggctgtcg tatcgccatc gcgctttcga aaaggatctg agccgatgaa
     1681 gcttctcgcc gccgccgccg tggccgcctt gctcgcctcc gccgggcccg gccatgccca
     1741 ggcgcccgcg gccgagccga aggtcagcgc gatgatcgtc tacggcgacg atccctgccc
     1801 gccgagcacc gacgacacga tcgtggtctg cgcccgcaag ccggaaggcg agcgctaccg
     1861 aatcccggag gtgctccgcg gcgatcccaa cgatcccaag aaccaggcct gggcggagcg
     1921 ggcgacggcg ctcgaatatg ccggccgcgg cggcatcggc agctgctcga cggtcggacc
     1981 gggcggcgcc agcggctgct tcaaccagct cgtccgcgag gcccgcgccg ccggccggac
     2041 cgccgacgag gtcaattgga acgccctcat cgagcaggcg cgccgcgagc gcctcggcaa
     2101 gatcgacgag caggccgaag ccgaggaagc cgacgccgtc gccgacgaag gcggcacgcc
     2161 gcgctgatct catcctcccc atcgccagat ggggaggata gggaaccctt cgccgctccg
     2221 gcccttcttc caggcagagc tgaaaggagg ccggtcatga aagccgccct gtcgattgcc
     2281 gcccttgccg ccgccggagt cgcggccgtg agcgtcgcgc ccgcccccgc cgaggcccag
     2341 ggcgcgcagc aagagcgcat ccgccgcctc gtcgtctacg gccgcgatcc ctgcccgcgc
     2401 gccgccgccg gcgaggacat cgtcatctgc gcccgccgcc ccgagac
//
LOCUS       DASVQZ010000292         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_637226, whole genome shotgun sequence.
ACCESSION   DASVQZ010000292 DASVQZ010000000
VERSION     DASVQZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637226"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VF547_07990"
     CDS             <1..928
                     /locus_tag="VF547_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX8262799.1"
                     /translation="MAVPEILPAHLSPPNSRRRASLRAERGGAVGFNAEASHRVIDMR
                     ECHILRPELFALVQPLRALLRRLKPSAAAGIRMTLCDQGVDLLLEKIEAEDLAAAEAL
                     GAFALDHGLARLALDDGYGAQTRWEPEPVTVTLGGVPVPLPHGAFLQATAEGESALVR
                     AVRACVGDARIVADLFAGLGTFALALEGRILAVEGARDAALALKAAAGRAQRPVFVDH
                     RDLYRRPLDLAELNRFEAVVLDPPRAGAKEQAALLARASVPRIAYVSCNPSTFARDSA
                     LLTEGGYRLEWVQPVGQFRWSTHVELVGAFSR"
     gene            complement(1041..1490)
                     /locus_tag="VF547_07995"
     CDS             complement(1041..1490)
                     /locus_tag="VF547_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX8262800.1"
                     /translation="MYFSTIIAPVVALVAWTLLIMAWMVVSRFAAMKRKGISLKGRVG
                     GRGGNALEGVVEDEVMWKAHNYMHLVEQPTLFYAVSLALAIGHGGGGYSTVLAWAYVA
                     LRIAHSLVQTTVNVITYRFILWALSSLALIGLTVQAVRMVHAHMLMA"
     gene            1636..>1857
                     /locus_tag="VF547_08000"
     CDS             1636..>1857
                     /locus_tag="VF547_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262801.1"
                     /translation="MKGIATTAIAAFLLAGAAQAQPRQAQRPPAQAQRPPAQVQRPAA
                     TAVPTRVTARTLTAICGQDRNACLTYVLGA"
BASE COUNT          268 a          676 c          638 g          275 t
ORIGIN      
        1 aatggcggtt ccggaaatct tgccggccca tctctccccg ccgaacagcc gccgccgggc
       61 ttcgctcagg gccgagcgcg gcggagcggt cggcttcaat gccgaggcca gccaccgggt
      121 catcgacatg cgcgaatgcc acatcctccg gccggagctg ttcgcgctcg tccagccgct
      181 tcgggcgctt ctccggcggt tgaagccgtc ggcggcggcc ggaatccgga tgaccctgtg
      241 cgaccagggc gtcgacctac tgctggagaa gatcgaagcc gaggatctcg ccgccgccga
      301 ggccctgggc gccttcgctc tcgaccacgg cctcgcccgg ctggcgctcg acgacggcta
      361 cggggcccag acccgctggg agccggagcc cgtcacagtc accctcggcg gggttccggt
      421 gcctctgccg cacggcgcct tcctccaggc gacggcggaa ggagagtcgg cgctcgtccg
      481 ggcggtgcgc gcctgcgtgg gcgatgcccg gatcgtcgcc gaccttttcg ccggcctcgg
      541 caccttcgcc ctcgctctcg aggggcggat cctcgcggtc gagggcgccc gcgacgcggc
      601 gctcgcgctc aaggccgcgg ccggtcgggc gcagaggccc gtcttcgtcg atcatcgcga
      661 cctctaccgc cgtccgctcg acctcgcgga gctgaaccgg ttcgaggccg tcgtcctcga
      721 cccgccccgg gcgggagcga aggagcaggc ggccctgctc gcccgggcgt cggtcccccg
      781 gatcgcctac gtctcctgca atccctccac cttcgcccgc gattccgccc ttctcaccga
      841 aggcggctat cgtctcgaat gggtgcagcc cgtcggccag ttccgctggt cgacccatgt
      901 cgagctggtc ggcgcctttt cgcggtaggg cacgatcgtc tcggttcgac gtgccgcccc
      961 gttctccggc gaaggccggg gcccagcggc gcgacgggcg agctggatcc cggccttcgc
     1021 cgggaaacag aggtcacccc ctaggccatg agcatgtgcg catggaccat ccgaaccgcc
     1081 tgcacggtca gcccgatcag cgccaaagag gagagcgccc aaaggatgaa ccggtaggtg
     1141 atgacgttga cggtcgtctg caccaggctg tgcgcgatcc tcagggcgac ataggcccag
     1201 gcgaggacgg tgctgtagcc gccgccgcca tggccgatcg cgagtgcgag ggagacggcg
     1261 tagaagagcg tcggctgctc gaccaggtgc atgtagttgt gcgccttcca catcacctcg
     1321 tcctcgacga ctccttcgag ggcgttgccg cctcggccgc cgacccggcc cttgagcgag
     1381 atgcctttgc gcttcatcgc cgcgaaccgg ctgacgacca tccaggccat gatcagaagg
     1441 gtccaggcga cgagagcgac gacgggcgct attatggtgc tgaagtacat gtttcgattc
     1501 cccctgttgc cgtcccagac tggcgctcgc gcgggcggga gtcaaagcgg ccttcagcgg
     1561 ccgcaaaagc ctgctagaca gagccgggct tggagaggcc gagcgccgag gggccgagcg
     1621 taaaggagtg aagcgatgaa ggggattgcg acgacggcca ttgcggcctt cctgctggcc
     1681 ggggcggccc aggcccagcc tcggcaggcc cagcgcccgc cggcccaggc gcagcggccg
     1741 ccggcgcagg tccagcgccc ggccgcgacc gccgtgccaa cccgagtgac cgcccggacc
     1801 ctgaccgcga tctgcggcca ggaccgcaac gcctgcctca cctacgtcct cggcgcg
//
LOCUS       DASVQZ010000293         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_637458, whole genome shotgun sequence.
ACCESSION   DASVQZ010000293 DASVQZ010000000
VERSION     DASVQZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637458"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VF547_08005"
     CDS             complement(<1..863)
                     /locus_tag="VF547_08005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX8262802.1"
                     /translation="MIRTSLFLLGFSAAIALPATAAAPPFETAAPVAYMVDLSSGAVL
                     YAKDPDRRIPPASMAKMMTTHLAFRMIKRGEAKLGQMCQVRPETWRQWHGPQAGSTMF
                     LSPGEQVSVENLLHGIVTLSGNDATVVLAECLAGTEQAFAAQMNQEAKRLGMANSNFG
                     NTNGWPDEGVTYTTARDLARLAAATIQETPDLYKRFYQRKEFTWGKTLGKGDPITQGN
                     RNPLLGRVPGADGLKTGHTAEAGYGFTGSAEQNGRRIVMVVAGLSSFNQRIEESVKFM
                     DWGFRAWKSQP"
     gene            complement(951..1994)
                     /locus_tag="VF547_08010"
     CDS             complement(951..1994)
                     /locus_tag="VF547_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX8262803.1"
                     /translation="MRLLRGLSALALAAAVVPLGGSAAAQTGLSQAGFEAYLPQLEAQ
                     AVRAGVSRATIASVFPNLVFSARTVQLDRAQPGGRPGSNPNPPFAPYRARHVTPTLIA
                     AGQRRYAANLTYLREIGRRYGVAPSVIVAIWGHETSYGAVTGNFDLLNSLASLAYDGR
                     RRQLFADEFVAALKMIDRGVPRSRLKGSWAGATGHPQFLPSVWLRLARDGDGDGRADI
                     WRSEPDALASIANYLSNAGWKPGVPWGLAVRVPPGLDRAALRSTLRAPRCPRVHERHS
                     RWLTIAEWRRLGLVSQNGIWPRDSEMASLIEPDGPGATAYLLTGNYRAILDYNCSNFY
                     ALSVGLLADAIAR"
     gene            2105..2194
                     /locus_tag="VF547_08015"
     tRNA            2105..2194
                     /locus_tag="VF547_08015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2139..2141,aa:Ser,seq:tga)
     gene            complement(2237..2905)
                     /locus_tag="VF547_08020"
     CDS             complement(2237..2905)
                     /locus_tag="VF547_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262804.1"
                     /translation="MAVWLVVPDRPMAPTAWIGPNRAITSARLEPPRLYENRTDVRTS
                     DGKLLVPAGMPLMGLVSRTQAACASEPRGDRGLAAAWWLGTNRFICLVDQDRDGAFDH
                     YFSLRTGPANLIIGAGRFPAVLQPLRPAAYVERNPATFAQAPRIYVSYSHFASLVNTL
                     IFHLCFAERRGLCLYPDVRTKREPLPQTFEMLGARFQVSAKEGDRLQITQLTEMVRQP
                     LFIR"
     gene            3261..4451
                     /locus_tag="VF547_08025"
     CDS             3261..4451
                     /locus_tag="VF547_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8262805.1"
                     /translation="MAEAAIGRFDWEDPFALDAQLSDEERIVRDTAKGYAQEKLQPRV
                     TEAFLEEEFDRQIMSEMGELGLLGATIPNEYGGAGLNYVSYGLVAREVERVDSGYRSA
                     MSVQSSLVMHPIHAYGSEAQRRRWLPGLARGELIGCFGLTEPDAGSDPGGMRTRAEKI
                     DGGYRLSGSKMWITNSPIADVFVVWAKSDAHDGKIRGFLLEKGMKGLTAPKIGMKLSL
                     RASITGEIVMDGVEVPEENLLPNASGLAGPFGCLNRARYGIGWGAMGAAEACYVAARR
                     YTLDRKQFGRPLASNQLVQLKLANMVTEITLGLQAALRVGRRMDEGELIPETISLIKR
                     NNVGKALDIARTARDMHGGNGISAEFQVMRHAMNLETVNTYEGTHDVHGLILGRAITG
                     ISAF"
BASE COUNT          753 a         1490 c         1660 g          689 t
ORIGIN      
        1 aagggctgcg acttccaggc gcggaagccc cagtccatga acttgaccga ttcctcgatt
       61 cgctggttga agctcgacag gccggcgacg accatgacga tccggcgccc gttctgctcg
      121 gccgagccgg tgaagccgta gccggcctcg gcggtatggc cggtcttgag gccgtcggcg
      181 ccggggaccc gcccgagaag cgggttgcgg ttgccctggg tgatcggatc gcccttgccc
      241 agcgtcttgc cccaggtgaa ttccttcctc tggtagaagc gcttgtagag gtcgggcgtc
      301 tcctggatgg tggcggcggc gagcctggcg agatcgcggg cggtggtgta ggtcacgcct
      361 tcgtccggcc agccgttggt gttgccgaaa ttggaattgg ccattccgag gcgcttggcc
      421 tcctggttca tctgcgcggc gaaggcctgc tcggtgccgg cgaggcattc ggcgagcacc
      481 accgtcgcat cgttgccgga caaggtgacg atgccgtgga gcagattctc gacgctgacc
      541 tgctcgcccg gcgacaggaa catggtcgag cccgcctgcg gtccgtgcca ttgccgccag
      601 gtctcggggc gcacctggca catctggccc aatttcgcct cgccgcgctt gatcatccgg
      661 aaggcgaggt gggtggtcat catcttcgcc atcgaggcgg gcgggatgcg ccggtcggga
      721 tccttggcgt agaggaccgc gccggagctg aggtcgacca tgtaggcgac cggggcggcg
      781 gtctcgaagg gcggcgccgc agccgtggcc ggaagcgcga tcgcggcgga gaaacctagc
      841 aggaacagac tggtacggat catagctaag acgatttccc tcaatgtcgg cgcatcgggc
      901 cgaagcgtcg gaccctgctt aacccctccc ctcgcgccgc gccagacgga tcagcgcgcg
      961 atcgcgtcgg cgagcagccc gaccgacaag gcgtagaagt tggagcaatt atagtcgagg
     1021 atggcgcggt agttgccggt gagcagatag gcggtcgcgc cgggcccgtc gggctcgatc
     1081 agcgacgcca tctccgaatc gcgcggccag atgccgttct ggctgaccag tccgaggcgg
     1141 cgccactcgg cgatcgtcag ccaccggctg tggcgctcgt ggactcgcgg gcagcgcggc
     1201 gcccgcaagg tgctgcgcag ggcggcgcga tcgaggccgg gcgggacgcg cacggccagg
     1261 ccccagggca cgcccggctt ccacccggca ttgctgagat aattggcgat cgaggcgagc
     1321 gcgtcgggct cgctgcgcca gatgtcggcg cggccgtcgc cgtcgccgtc ccgggcgagg
     1381 cgcagccaga ccgaagggag gaattgcgga tgcccggtgg cgcctgccca gctgcccttg
     1441 aggcggctcc tcggcacgcc gcggtcgatc atcttcaggg cggcgacgaa ctcgtcggcg
     1501 aagagctgcc ggcggcggcc gtcataggcg agcgaggcga gcgagttcag caggtcgaaa
     1561 ttgccggtga cagcgccgta gctggtctcg tggccccaga tggcgacgat caccgacgga
     1621 gcgactccgt agcgccggcc gatctcgcgc agataggtca ggttggcggc atagcgccgc
     1681 tggccggcgg cgatcagggt cggcgtgacg tggcgggcgc ggtagggcgc gaagggcggg
     1741 ttggggttgc tcccgggccg gccgccgggc tgggcgcggt cgagctggac ggtgcgggcg
     1801 ctgaagacga ggtttggaaa gacgctggcg atcgtcgctc ggctgactcc cgcgcgaacg
     1861 gcctgcgcct ccagctgggg cagataggct tcgaagccgg cctgggacag gccggtctgg
     1921 gccgccgccg agccaccgag cggaacgacc gccgccgcca gcgccagcgc cgacaacccc
     1981 cgcaacaaac gcatactcga tctcccgctt gcctcactga tgccacagcc tccgtccggc
     2041 ggatactcgg ctcggcgcgc gggttgcacg agcggccccg cccggcctag agcggcgcca
     2101 tcttggacag gtggccgagt ggtttaaggc agcggtcttg aaaaccgccg tgggttcacg
     2161 cccaccgtgg gttcgaatcc caccctgtcc gccatcgccg gatttccatt gcggggcgtg
     2221 cacggcatcc tttggctcag cggatgaaga ggggctgcct gaccatctcg gtcaactggg
     2281 tgatctgcag ccgatcgcct tccttggctg agacctggaa gcgcgcgccc agcatttcga
     2341 aggtctgcgg gagcggctcg cgtttggtcc ggacgtccgg atacaggcac agaccccgcc
     2401 tctcggcgaa acagagatga aagatgaggg tgttaaccag cgacgcgaaa tgggagtagc
     2461 tgacgtagat ccgaggcgcc tgggcgaagg tcgcgggatt gcgttcgaca taggccgccg
     2521 gccgcaaggg ctggagaacg gccgggaacc gcccggcccc tatgatgaga ttggcggggc
     2581 cggtgcgaag cgagaaataa tggtcgaagg cgccgtcgcg gtcctggtcg accaggcaga
     2641 tgaagcgatt ggtgccgagc caccaggccg cggccagacc gcggtccccg cgcggctcgg
     2701 acgcgcaggc cgcctgggtc cgcgacacca ggcccatcag cggcatgccc gccggcacca
     2761 gcagcttccc gtccgaagtc ctgacgtcgg tccgattctc gtacagtcgc ggcggttcga
     2821 gccgggcgct ggtgatggcc cggttcggac cgatccaggc cgtcggggcc atcggccgat
     2881 ccgggacgac cagccagacc gccatgtagc ggtcccgctc cgcgggggcg tccgattgtg
     2941 gcggcggggg cggcggagag ggcggctgag cgacggcaga ggcagcggcc gaaccgatcg
     3001 caaaacccag aagaccgccc cgagcgaagc ggaaaaaaga cgttgccatt cgaaaggctt
     3061 cccccgaccc ggcccgcacc ttgccatcgg cggacggccg tgtcgaggcc gcgctcgcgc
     3121 cgtctgcggc catgacacgg ggctccgcgg ggatcctccc gaaccgttcg tgcaggggga
     3181 gccgcttgaa ctcgccgtaa ctgacgctat cgcacacgac cgggacggag gcccccgtcc
     3241 gccaagcaaa agggaatgag atggccgaag cggcgattgg caggttcgac tgggaggacc
     3301 cgttcgcgct cgacgcgcag ctctccgacg aggagcggat cgtccgcgac acggccaagg
     3361 gctatgcgca ggagaaattg cagccgcggg tgaccgaggc cttcctcgag gaagagttcg
     3421 accgccagat catgtccgag atgggcgagc tgggcctgct cggggcgacc atcccgaacg
     3481 aatatggcgg cgccgggctc aactacgtca gctacgggct ggtggcgcgg gaggtcgagc
     3541 gggtcgacag cggctatcgc tccgcgatga gcgtccagtc ctcgctcgtc atgcacccga
     3601 tccatgccta cggctccgag gcgcagaggc gcagatggct gcccggcctg gccaggggcg
     3661 agctgatcgg ctgcttcggg ctcaccgagc cggacgccgg ctcggatccc ggcgggatgc
     3721 gcacccgggc ggagaagatc gacggcggct accgtctgtc cggctcgaag atgtggatca
     3781 ccaactcgcc gatcgccgac gtcttcgtcg tctgggccaa gtccgacgcg cacgacggga
     3841 agatccgcgg cttcctgctc gagaagggga tgaaggggct gacggcgccg aagatcggga
     3901 tgaagctcag cctgcgcgcc tcgatcaccg gcgagatcgt catggacggg gtcgaggtgc
     3961 cggaggagaa tctccttccc aatgccagcg gcctcgccgg cccgttcggc tgcctcaacc
     4021 gggcgcgcta cggcatcggc tggggcgcga tgggcgcggc cgaggcctgc tacgtggcgg
     4081 cccggcgcta cacgctcgac cgcaagcagt tcgggcggcc gctggcgtcg aaccagctgg
     4141 tccagctcaa gctcgccaac atggtcaccg agatcacgct cggcctgcag gcggcgctgc
     4201 gggtgggacg gcggatggac gaaggcgagc tgatcccgga gacgatcagc ctgatcaagc
     4261 gcaacaatgt cggcaaggcg ctcgacatcg cccgtaccgc ccgcgacatg catggcggca
     4321 acggcatctc ggccgagttc caggtgatgc gccacgcgat gaacctggag acggtcaaca
     4381 cctacgaggg cacgcacgac gtccacggcc tgatcctcgg gcgggcgatc accgggattt
     4441 cggcgttttg accgtctttg cgagcgaagc gaagcaatcc agcggctcgg gcgcccaaat
     4501 ctggattgct tcgtcgcttc gctcctcgca atgacgaagg gggctcatgg ctgggcttta
     4561 cctgccttcc atcctatgta gcccccgcca tg
//
LOCUS       DASVQZ010000294         7937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_351216, whole genome shotgun sequence.
ACCESSION   DASVQZ010000294 DASVQZ010000000
VERSION     DASVQZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351216"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /gene="fabZ"
                     /locus_tag="VF547_08030"
     CDS             complement(<1..245)
                     /gene="fabZ"
                     /locus_tag="VF547_08030"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX8262806.1"
                     /translation="MAALPHRYPFLLIDRVEELIPKKRIVAIKAVTINEPFFQGHFPD
                     RPIMPGVLLVEAMAQASGVLAVESLGLAGTGKLVYFMA"
     gene            complement(296..907)
                     /locus_tag="VF547_08035"
     CDS             complement(296..907)
                     /locus_tag="VF547_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX8262807.1"
                     /translation="MNKLAFGAAIAALSLALPGTALAQRTPGAVIVVVDTDRVFRECT
                     ACRAAQTQLQGMITSARTRAQQLGQPLQTEAQSIEQAAAALRNQTGAARAAAETALNT
                     RVQQFQTRQTTAQQEVARLEQNIQSTQQNVLRQINERLNPIITQVMNARNANIALDVN
                     ATLARSNTLDVTNDVLTQLNAGLPSVSVTPLPQAPAQPQPQGR"
     gene            complement(907..3558)
                     /gene="bamA"
                     /locus_tag="VF547_08040"
     CDS             complement(907..3558)
                     /gene="bamA"
                     /locus_tag="VF547_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX8262808.1"
                     /translation="MTPTNKFTNGRRLCSVLLVGTILGGFALPAQAQEATPVSTAPVA
                     PVAPAPQPEASVIRSIEVTGNQRLEPETVVSYVKLRVGEAYDRERLDEALRDLYGTDL
                     FADVSIRDNQGNLVIEVRESPVVNRIVLEGNKRLKDDKITPEIRLAPRQIFSRAKARA
                     DVARIIELYRRQGRFAATVEPKIVQLEQNRVDVVYEINEGPKSRIRQINILGNEKFGD
                     GELRSEMVTKRTSMLSFLGSGDVYDPDRLAFDQQKLRMFYLTHGYADFRVVSAVAELT
                     PDRRDFIITYVVEEGERYKFGDVKVESAIRDFSSENLTRLLPMKSGDWYNAKQVEDTV
                     TSLNESAGLFGYAFADVQPNFQRDKDTKTMSVTFNVAETPRVYVERIDVSGNTNTRDK
                     VIRREFRLAEGDPFNSIRVKRSRDRIQSLGFFQENLEVEQTQGSASDRVILSTALEEK
                     ATGQLQVSAGFSSLERFLLNLSVEQRNFRGMAQTLRASINYSSYSKSVELGFTEPYVF
                     DRNIALGFDLFRRDYSSFNFVNNNRNTTYKQVSTGGQLRGGLPLTEFIQIGGRYGLNF
                     DNVTLDESAFFTDPDGAGPLPAFCDPLRVGRYLCDAIGKRTTSSIGYSLLYDTTDNRL
                     RPTRGQRLTLNQDFAGLGGDVKYLRTRANAAKYWRIGGGFIFSLSGEGGYIHSFENSP
                     GAGQDPVRLVDRFFLGEPQIRGFDIRGVGPRVQRVDFVKDEDGNFVLETDRNQIVDDA
                     LGGRAYYVGRAEVEIPVSSSFRELGLRPSIFVDVGALFSLKNPVTQSILPGDPRTINR
                     CVDANGGSTTAPDNLQCPTGSQLVAGIQPFVENYLGDTAKPRLSIGFGVNWNSPFGPF
                     RIDIAKALLKADGDDTKLLTFNVGTAF"
     gene            complement(3638..4771)
                     /gene="rseP"
                     /locus_tag="VF547_08045"
     CDS             complement(3638..4771)
                     /gene="rseP"
                     /locus_tag="VF547_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX8262809.1"
                     /translation="MNNPGFVWTVLSFLLVIGPLIFVHELGHYFVGRWFGVHAETFSI
                     GFGREVAGWSDSRGTRWKVGWLPLGGYVKFAGDMNAAGQPSPEWLALPAEERARTFQA
                     KPVWQRFLIVLAGPMTNFLAAIVILTGFNLAYGVPKTPAVIGTVQAQSAAEQTGLRPG
                     DRILRVDGSSVSRFEDILPIVALKTGGAVVLDIERQGRPLRVAVVPRMTSETDEFGNQ
                     MRRPVLGIKPPGEQLLTPVSAPGAVAASISQCWVVVSTTLKGLGQIILGSRSVKELGG
                     PLKIAKFSGEQASMGMLHFLWFIAVISINLGFINLLPIPMLDGGHLFFYGIEAVRRKP
                     LRPEAQEWAFRTGLAALLALMILVTFNDLASFGLWSKLGGLID"
     gene            complement(4768..5934)
                     /locus_tag="VF547_08050"
     CDS             complement(4768..5934)
                     /locus_tag="VF547_08050"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX8262810.1"
                     /translation="MTSARSVTILGATGSVGRSTLDLIERDRERYEVLAVTARSDVAG
                     LAEAARRVGAARAVIADPSLLQPLREALEGSGSEAAAGEDAILEAATAGADWTMAAIV
                     GTAGLSPTMAAIRGGRTVALANKEALVSAGHLMIEAATASGAELLPVDSEHNAIFQCF
                     DRARPEGVRRIILTASGGPFREKSLDEMRSATPSEAIAHPNWSMGAKISVDCATLMNK
                     GLELIEAFHLFPLPVDAFDVVIHPQSVVHSLVEYVDGSVLAQLGSPDMRIPIAYTLAW
                     PERMATPCERLDLVRTASLSFEAPDLERFPALRLARQALVEGGARPAVLNAANEVAVG
                     AFLEGRLGFLDIASIAAETLQCYAPAAPASVEEVLEVDREARATAEALVQKVMR"
     gene            complement(5931..6752)
                     /locus_tag="VF547_08055"
     CDS             complement(5931..6752)
                     /locus_tag="VF547_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX8262811.1"
                     /translation="MSDDAALQSDLLTRFAAGVVMIAVACVAIYLGGWPFRLLAAAAA
                     AAMMIEWGNMHRVAYGWTGLGVALLAAPTLILPEILFPAVERDPAEISPELFDQAWMG
                     FAGIAAAALLYTLLSRRLTLGWGFLYIALPSFALLVLSWAWFELVFWLMLVTWATDIF
                     AYFAGRSIGGPKLAPKISPNKTWAGLLGGMAGAALVGAGAAWLFGIGEPFFWLGAPMG
                     LLAQLGDLYESRVKRRLGVKDSGNLLPGHGGVLDRLDGLLPVVLATLVVLLASLA"
     gene            complement(6749..7498)
                     /locus_tag="VF547_08060"
     CDS             complement(6749..7498)
                     /locus_tag="VF547_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX8262812.1"
                     /translation="MTARPAKAAPESAGGGIPPAPRHVAIIMDGNGRWAKARGLPRAA
                     GHRQGAEAARKVLRAAGEAGIECLTLYAFSSENWRRPQDEINDLMGLLRFYISRELEA
                     LHREGVRLKILGDHSAFQDDVATLIDQAVARTAGNGRMTLAIALNYGSRAELVQAARR
                     IAERAARGEIEPSEVDEAMVDSSLDTAGLPPLDLLVRTSGEQRLSNFLLWQAAYAELA
                     FVDTLWPDFDGDALRAVLADYSARERRFGGR"
     gene            complement(7495..>7937)
                     /gene="frr"
                     /locus_tag="VF547_08065"
     CDS             complement(7495..>7937)
                     /gene="frr"
                     /locus_tag="VF547_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX8262813.1"
                     /translation="VTVDVYGANMPLNQVATVSAPEPRMLSVQVWDRSNVNAVEKAIR
                     AAGLGLNPISDGQNLRLPIPDLTEERRKELAKLAHQYAEKARIAVRNVRRDGMEALKT
                     DEKKHEISQDEHKRLEVEVQKMTDETIKEIDAAMAAKEKEILQR"
BASE COUNT         1241 a         2590 c         2829 g         1277 t
ORIGIN      
        1 gccatgaaat agacgagctt gccggtgccg gcgaggccga gcgattcgac cgcgagcacc
       61 cccgaagcct gcgccatcgc ctcgacgagc agcaccccgg gcatgatcgg ccggtccggg
      121 aaatggccct ggaagaaggg ctcgttgatc gtcaccgcct tgatcgcgac gatccgcttc
      181 ttcgggatca gctcctcgac ccggtcgatg agcaggaagg ggtagcgatg cggcagcgcc
      241 gccatcaccc gccggatatc gagcgggccg atggcggcgt ctgccggctc ggagttcacc
      301 ggccctgagg ctggggctgc gccggcgcct gcggcagcgg cgtcacgctg accgagggca
      361 ggcccgcgtt cagctgggtg aggacgtcgt tggtgacgtc gagcgtgttc gagcgcgcca
      421 gggtcgcgtt gacgtcgagc gcgatgttgg cgttgcgcgc gttcatcacc tgggtgatga
      481 tcgggttgag ccgctcgttg atctggcgaa gcacgttctg ctgggtcgac tggatgttct
      541 gctcgagccg ggcgacttcc tgctgggccg tggtctggcg cgtctggaac tgctgcactc
      601 gggtgttgag cgcggtctcg gcggcggctc gggcggcgcc ggtctggttg cggagcgcgg
      661 cggccgcctg ctcgatcgac tgggcctcgg tctgcagcgg ctggccgagc tgctgggcgc
      721 gggtgcgggc cgaggtgatc atgccctgga gctgggtctg ggcggcgcgg caggcggtgc
      781 actcgcggaa gactcggtcg gtatcgacga cgacgatcac cgccccgggg gtgcgctgcg
      841 ccagggccgt gcccggcagc gccagcgaaa gcgcggcaat ggccgcgccg aaagcgagtt
      901 tgttcatcag aatgcggtcc ctacgttgaa ggtgagaagc ttggtgtcat ccccgtccgc
      961 ctttaagagc gccttggcta tatcgatcct gaacggaccg aaaggcgagt tccagttgac
     1021 cccgaagccg atcgacaggc gcggctttgc ggtgtcgccg agataattct cgacgaaggg
     1081 ctggatgccg gcgacgagct ggctgccggt cgggcactgg agattgtccg gcgcggtggt
     1141 cgagccgcca ttggcgtcga cgcagcggtt gatcgtgcgc gggtcgcccg ggaggatcga
     1201 ctgggtgacg ggattcttga gcgagaagag cgctccgacg tcgacgaaga tggacggcct
     1261 gagccccagc tcgcggaagc tcgagctgac cgggatctcg acctccgcgc ggccgacata
     1321 gtaggcgcgg ccgccgagcg cgtcgtcgac gatctggttg cggtcggtct cgagcacgaa
     1381 attgccgtcc tcgtccttga cgaagtcgac gcgctggacc cgggggccga cgccgcggat
     1441 gtcgaagccg cggatctgcg gctcgccgag gaagaagcgg tcgaccagcc ggaccgggtc
     1501 ctggccggcg ccggggctgt tctcgaagct gtggatgtag ccgccctcgc cgctgagcga
     1561 gaagatgaag ccgccgccga tccgccagta tttggcggcg ttggcgcggg tccgcagata
     1621 tttgacgtcg ccgccgaggc cggcgaagtc ctggttcagc gtcaggcgct ggccgcgggt
     1681 cggacggaga cggttgtcgg tcgtgtcgta gagcagcgaa tagccgatcg acgaggtggt
     1741 gcgcttgccg atcgcgtcgc acaggtagcg gccgacccgg agcggatcgc agaaggcagg
     1801 gagcggaccg gcgccgtcgg gatcggtgaa gaaggcggat tcgtcgagcg tcacgttgtc
     1861 gaagttgagg ccgtagcggc cgccgatctg gatgaattcc gtgagcggca atccgccgcg
     1921 aagctggccg ccggtgctga cctgcttgta ggtggtgttg cggttgttgt tgacgaagtt
     1981 gaagctcgaa taatcgcgcc ggaacaggtc gaagccgagc gcgatgttgc ggtcgaacac
     2041 atagggttcg gtgaagccca gctcgaccga tttcgagtag ctcgaatagt tgatcgaggc
     2101 gcgcagcgtc tgggccatgc cgcggaagtt gcgctgctcg accgacaggt tgagaaggaa
     2161 gcgctccagg ctcgagaagc cggccgaaac ctggagctgg ccggtcgcct tctcctccag
     2221 ggccgtcgac aggatcaccc ggtcggacgc cgagccctgg gtctgctcga cctccagatt
     2281 ctcctggaag aagccgagcg actggattcg gtcgcgcgag cgcttcaccc ggatcgagtt
     2341 gaacggatcg ccctccgcca gccggaactc gcgccggatc accttgtcgc gggtgttggt
     2401 gttgccgctc acgtcgatcc gctcgacata gacgcgcggc gtctcggcga cgttgaacgt
     2461 caccgacatc gtcttggtgt ccttgtcgcg ctggaaattg ggctggacgt cggcgaaggc
     2521 gtagccgaac aagcccgcgc tctcgttgag cgaggtcacc gtgtcctcga cctgcttggc
     2581 gttgtaccag tcgccggact tcatcggcag cagacgggtc aggttctcgc tgctgaagtc
     2641 gcggatcgcg ctctcgacct tgacgtcgcc gaacttgtag cgctcgcctt cctcgacgac
     2701 gtaggtgatg atgaagtcgc ggcggtccgg agtcagctcc gccacggccg agacgacgcg
     2761 gaaatccgca tagccgtggg tgaggtagaa catcctcagc ttctgctggt cgaaggccag
     2821 ccggtcggga tcgtagacgt cgcccgagcc gaggaagctc agcatcgagg tgcgcttggt
     2881 caccatctcc gagcgcagct cgccgtcgcc gaacttctcg ttgccgagga tgttgatctg
     2941 gcggatgcgc gacttggggc cctcgttgat ctcgtagacg acgtcgacgc ggttctgctc
     3001 gagctggacg atcttgggct cgacggtggc ggcgaagcgg ccctggcggc ggtagagctc
     3061 gatgatcctg gccacgtcgg cgcgcgcctt ggcgcggctg aatatctgcc tcggcgcaag
     3121 ccggatctcg ggcgtgatct tgtcgtcctt gagccgcttg ttgccttcga gcacgatgcg
     3181 gttgacgacg gggctctcgc gcacctcgat gacgaggttg ccctggttgt cgcggatcga
     3241 gacgtcggcg aacaggtcgg tgccgtaaag gtccctgagc gcctcgtcga gccgctcgcg
     3301 gtcataggcc tcgccgaccc tcagcttcac gtaggaaacc accgtctcgg gctcgagccg
     3361 ctggttgccg gtcacctcga tcgagcggat cacgctcgcc tcgggctggg gcgccggcgc
     3421 gacgggcgcc acgggcgccg tcgacaccgg agtcgcctcc tgggcctgcg ccggaagcgc
     3481 gaagccgccg aggatggtgc cgacgagcag gacagaacaa agccgccggc cgttcgtaaa
     3541 tttgtttgtg ggtgtcacgc gtccccgccc ttagactgaa cggacggcgc gagcggccat
     3601 cctgtttccc tgtcgcccct gccctgcccg atcacgctca gtcgatcaac ccgccaagct
     3661 tcgaccacag tccaaaagac gccagatcgt tgaaagtgac caggatcatc agggccagaa
     3721 gcgccgccaa gccggtcctg aacgcccatt cctgtgcctc cggccgcaac ggtttccggc
     3781 ggacggcctc gatcccgtag aagaagaggt ggccgccgtc gagcatcggg attggcaaca
     3841 ggttgatgaa tcccagatta attgagatca ccgcgatgaa ccacaggaag tggagcatcc
     3901 ccatgctcgc ctgctcgccc gagaatttgg cgatcttgag cggtccgccg agctccttca
     3961 ccgaccggct gccgaggatg atctggccga gccccttcag ggtggtgctg acgacgaccc
     4021 agcattggct gatcgaggcg gcgacggcgc caggagcgga gaccggggtc agcagctgct
     4081 cgccgggcgg cttgatgccg agcaccgggc ggcgcatctg gttgccgaac tcatcggtct
     4141 ccgaggtcat ccgcggcacg accgcgaccc tcagcgggcg tccctggcgc tcgatgtcca
     4201 ggaccacggc cccccccgtc ttgagggcga cgatcggcaa tatgtcctcg aaccgggaga
     4261 ccgacgaccc gtcgacccgc aggatgcggt cgccgggccg gagcccggtc tgctcggctg
     4321 cggattgcgc ctggaccgtg ccgatcaccg ccggagtctt cggcactccg taggccaggt
     4381 tgaacccggt caggatcacg atggcggcga ggaagttggt catcgggccg gcgaggacga
     4441 tcaggaagcg ctgccagacc ggcttggcct ggaaggtgcg cgcccgctcc tcggcgggaa
     4501 gggccagcca ttccggcgag ggctggccgg cggcgttcat gtcgccggcg aacttgacgt
     4561 agccgcccag cggcagccag ccgaccttcc agcgcgtgcc gcggctgtcg ctccagcccg
     4621 ccacctcgcg tccgaagccg atcgagaagg tctcggcatg caccccgaac cagcggccga
     4681 cgaaataatg gccgagctcg tgaacgaaga tcagcggccc gatgacgagc aggaagctga
     4741 ggaccgtcca gacgaaaccg ggattgttca acgcatcacc ttttgaacca gggcttcggc
     4801 ggtcgcgcgc gcctcgcggt cgacctccag cacttcctcg accgaggcgg gcgcggccgg
     4861 cgcgtagcat tgcagagtct cggcggcgat ggaagcgatg tcgaggaagc cgaggcgtcc
     4921 ctcgaggaag gcgccgacgg cgacctcgtt ggcggcgttg agcaccgccg gcctcgcccc
     4981 gccctcgacc agcgcctgcc gggcgaggcg gagcgccggg aaacgctcga gatcgggcgc
     5041 ttcgaagctc aggctcgcgg tcctgaccag gtcgagccgc tcgcagggcg tcgccatccg
     5101 ctccggccag gcgagcgtat aggcgatcgg gatccgcatg tcgggagaac ccagctgggc
     5161 caggaccgat ccgtcgacat attcgacgag gctgtggacc accgactgcg ggtggatgac
     5221 gacgtcgaac gcatcgaccg gaagcgggaa gaggtggaag gcctcgatca gctccaggcc
     5281 cttgttcatc agcgtcgcgc aatcgaccga gatcttggcg cccatcgacc aattggggtg
     5341 ggcgatcgcc tcgctcgggg tggcggagcg catctcgtcc aggctcttct cgcggaacgg
     5401 gccgccgctg gcggtcagga tgatgcggcg caccccctcc ggccgagccc ggtcgaagca
     5461 ttggaagatc gcattatgct cggaatccac cggcaggagc tccgccccgc tcgctgtcgc
     5521 cgcctcgatc atcaggtggc cggccgagac gagggcctcc ttgttggcca gggcgacggt
     5581 gcggccgccg cggatcgcgg ccatggtcgg gctcagtccg gcggtgccga cgatcgcggc
     5641 catcgtccag tcggcgcccg ccgtcgcggc ctctaggatg gcatcctcgc ccgccgccgc
     5701 ctcgctgccg cttccctcca gcgcctcgcg caagggctgg agaagggacg ggtcggcgat
     5761 caccgcccgt gccgcgccga cccggcgggc ggcttcggcc aggcccgcga cgtcgcttcg
     5821 ggcggtgacg gcgagcacct cgtagcgctc gcggtcgcgc tcgatcaggt cgagcgtcga
     5881 gcggccgacg gagccggtgg cgccgaggat ggtgacgctg cgggcggaag tcacgcgagg
     5941 ctggctagca ggacgacgag cgtggcgagg acgaccggga gaaggccgtc cagccggtcg
     6001 aggactccgc catggccggg aagcaggttg ccgctgtcct tgaccccgag ccggcgcttg
     6061 acccggcttt cgtagaggtc gccgagctgc gcgagcaggc ccatcggtgc gccgagccag
     6121 aagaagggct cgccgatccc gaacagccag gcggcgcccg cgccgaccag ggcggcgccg
     6181 gccatgccgc cgagcagccc ggcccaggtc ttgttcgggc tgatcttcgg agcgagcttg
     6241 ggcccgccga tcgaccggcc ggcgaaatag gcgaagatgt cggtcgccca ggtcaccagc
     6301 atcagccaga agaccagctc gaaccaggcc cagctcagaa cgagcagcgc gaagctcggc
     6361 agggcgatgt agaggaagcc ccagcccagg gtcagccggc ggctgagcag cgtgtagagc
     6421 agggcggcgg cggcgattcc ggcgaagccc atccaggcct ggtcgaacag ctcgggcgag
     6481 atctcggccg gatcgcgctc gacggcggga aacaggattt ccggaaggat cagcgtgggc
     6541 gcggcgagca gggcgacccc gagccccgtc cagccatagg ccacccggtg catgttgccc
     6601 cattcgatca tcatcgccgc ggcggcagcg gcggcgagga ggcggaacgg ccagccgccc
     6661 agatagatgg cgacgcaggc gacggcgatc atgaccacgc cggccgcgaa gcgcgtcagc
     6721 aggtccgact ggagggcggc gtcgtcgctc accggccgcc gaaccggcgc tcgcgggccg
     6781 aatagtcggc cagcaccgcg cgaagcgcgt cgccgtcgaa atccggccac aaggtgtcga
     6841 cgaaggcgag ctcggcatag gccgcctgcc agagcaggaa gttggacagg cgctgctccc
     6901 cggaggtgcg gacgagaagg tcgagcggcg gcaggccggc ggtgtcgagc gaggaatcga
     6961 ccatcgcctc gtccacctcg gacggctcga tctcgccgcg cgcggcgcgc tccgcgatcc
     7021 gccgcgcggc ctggaccagc tcggcccgcg atccatagtt gagcgcgatc gccagcgtca
     7081 tccggccatt gcccgccgtc cgcgccaccg cctgatcgat cagcgtcgca acatcgtcct
     7141 ggaaggcact gtgatcgccc aggattttca ggcggacccc ttccctgtga agggcttcga
     7201 gctcgcgcga tatgtagaag cgcaaaagcc ccatgagatc gttgatctcg tcctgcggcc
     7261 ggcgccagtt ctccgacgag aaggcgtaga gcgtgaggca ttcgatgccc gcctcgccgg
     7321 ccgcccggag caccttgcgt gcggcctcgg cgccctggcg gtggccggcg gcgcggggca
     7381 ggccccgggc cttcgcccag cggccgttgc cgtccatgat gatggcgacg tgccggggcg
     7441 ccggcgggat gcctccgcct gcgctttccg gcgccgcctt ggccggccgg gcggtcaccg
     7501 ctggaggatt tccttttcct tggccgccat cgccgcgtcg atctccttga tcgtctcgtc
     7561 ggtcatcttc tggacctcga cctctaggcg cttgtgctcg tcctggctga tctcgtgctt
     7621 cttctcgtcg gtcttcagcg cctccatgcc gtcgcggcgg acgttgcgca cggcgatcct
     7681 cgccttctcc gcatattggt gggcgagctt ggcgagctcc ttgcggcgct cctcggtcag
     7741 gtcggggatg ggcaggcgca gattctggcc gtcgctgatc gggttcaggc ccaggcccgc
     7801 cgcccgaatc gccttttcca ccgcgttgac gttcgagcgg tcccagacct gcaccgacag
     7861 catccgcggc tccggcgcgg agacggtggc gacctggttg agcggcatgt tggcgccgta
     7921 gacgtcgacc gtcaccg
//
LOCUS       DASVQZ010000295         9885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_447865, whole genome shotgun sequence.
ACCESSION   DASVQZ010000295 DASVQZ010000000
VERSION     DASVQZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447865"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VF547_08070"
     CDS             <1..624
                     /locus_tag="VF547_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEX8262814.1"
                     /translation="STARLGTEVQTRLWPRVPVGTRPAPPAPMSFALPARQASLLLSH
                     SAQPRPWATVSVRAAVPLTAPAFAGYRVGREVSFLERKRPGAVSAGDVLKVRITVEAP
                     VDRTWVVVEDPVPAGASVISGGGGQSALLAARASGGSGIWPSYIERGNDAWRAYYGWL
                     PQGRTTVEYVLRINGAGRFQLPPTRVEAMYSPEIHAALPNRPVVVTP"
     gene            830..2920
                     /gene="pbpC"
                     /locus_tag="VF547_08075"
     CDS             830..2920
                     /gene="pbpC"
                     /locus_tag="VF547_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX8262815.1"
                     /translation="MLCLLLPLLVIGALAAWTRPPDLPSYERARGDWRASEAWLRGRD
                     GRLLQTVRIDFAVRRLEWVPIERISPSVREAVVASEDRHFERHGGVDWSAFAGSAWAA
                     LRGRPARGASTISMQVAGFLAPELGGPGGRSFANKLRQARAARALESEWTKPQILEAY
                     LNLATFRGETQGVGAAARALFGRAPDALGREDALLLAALLPAPQAPPSAVARRACRIG
                     RVADCATMTARAEAMTGPGRRLADDPGLAPHLAYRLLKKPGQTAVTTIDPQVQALAAG
                     ALRRQLIGLGGTRARDGAVIVIDNATAEVLAYVGGVGGASTAPAVDNASAPRQAGSTL
                     KPFLYGQAIERGFITAASILDDSPVQLDTASGLYVPRDYDNSFKGPVSVRTALAGSLN
                     VPAVRALLLNGVEEFRDRLWDIGYRGLTEDGDYYGYSLALGSAEVTLMQQAAAYRALA
                     DGGRYRPLRLRPDDPAGRGKAVADPVAAWIVADMMADADARAVTFGIDSALRLPFWAA
                     AKTGTSKAMRDNWCIGFSDRYTVAVWIGNSEGDSMSRVSGTSGAAPIWRDVMLAIHRG
                     RPGRAPPRPAGLEARAVVFADGTEPARPEYFLRGTAQSSIEGAPAAARRPRIANPVSG
                     AVYALDPDIPMARQRIRLTATGAAGGERMLLDGRDVGAAQDGPMVLPAPGAHRLALVD
                     ERGRTLDRSLFSVR"
     gene            3128..>9885
                     /locus_tag="VF547_08080"
     CDS             3128..>9885
                     /locus_tag="VF547_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX8262816.1"
                     /translation="MVGQRDESKKPPRNRIEIPKIELPKGGGAVRGIDEKFRVNSANG
                     TSSFSIPLPFHPGRDAFSPGLSIAYDSGSGMGLFGLGWSLDLPSISRSTGKRLPAYDD
                     ARESDDFQLTGFEDLVPVLRPAGGGALEPDVEQIGDHRIHRYRPRTEGAFARIERIHR
                     AGEAAAYWRVMPGDGTTTVFGRTAAARIADPEAGHRVFRWLPEWRFDDRGNCIEYVYK
                     PEDLANVPKLASEARRHEGEAAFANRHIKRILYGNRVPYDPGAAAPAHDPLPPAGLNH
                     FFQALFDYGEHGDGLDPAETRDWMARGDPWSDRRPGFDMRSYRLCRRILFLNRFEELD
                     PLAAEPPWVAVKGLEFTYVGADFATPAAPPIECERLTAVAEAGYRPDGAGGIGRTALA
                     PMRFDYHPAAIDTAVRRTPRKDLPNAPDGLMGEVHAVDLYGDGVPGLLSEREAEWLYA
                     RNLGGGRFGNAVPVWEKPSFAGIASGVLQVRDLEADGSKEIVIEQPGLAGYFPLDEGG
                     RYGPMVAFARPAVVPTPAARRRLIDLDGDGRPEIVIAGDSELHYLQSRGRRGYGRWCS
                     APYPPGEGPRRLLIADEEEGIHLADMTGDGLSDLVRVRSDSVAYWPNLGFGRFGPLVE
                     MERCPVLDSPERFDPRRVQFADLGGTGAADLVYDSDLGPVAAVNLCGNGWGPTLRFPG
                     LAGPQTATRLAVMDLLAQGTPCLVRSSSLPADAGAPLAWIDLYHGRKPYLLETVDNGA
                     GKTVAIRYRSSVQFQLEDEAAGRPWVTRLSFPVACVARTETRDSVTNSLFVTTYRYRH
                     GYYDHRDREFRGFAFVEEIDSEDYSALAGAEAGNAPAPELHEPPTRTRRWFHTGAFPG
                     WSLSQRLAAEFAPLPAAAAGLEGGVSEELPPGGPGDRIASHRVLKSKPLRTEVCVLDG
                     TAEEDLPYRITTHGFWIRLVQPPDGNRYGVHQAIESETVEIHMERDSADPRISHRLTL
                     AVDDHGLPERSAEIAYGRAAPPAGLDAQIAAAQQRSHVTVQIVRRTPDVDQPAFRRLR
                     TPFETASFELTGAAPAGTAWTASALDADFLGAAPRDYASPVPAGPARRLIELERIVFA
                     DDADPNLPLAFGTMGARGIVHQSYRLAFDSTLVQALYGTDVAPADLSSAAYLRESALV
                     AAGLFPPGEAGRWWTASGHVEYGPNPQAAFFRPTAYVDPVGQRTEVREYRDYFLLIDR
                     IRDAAGNDVTVTAFDWFVVAPSAVRDINGNISAVAYDRLGLVAGSALMGKGAEGDTLA
                     GFDPNPSDAAVDAFFADPVGEARALLGAATTRVVIDHRHRPNWSAVIGRAMHEADEAA
                     AGVLGQLQIAFDYSDGFGRVALRKLLAEPGEALVMVDTADGPKAISVDSGAAPRWVGS
                     GRSVANNKGDVVLAFNPYFALFHGYDDADALVAAGHSKRSHYDAFGRLVEVEHPDGSR
                     ELSEIAAWSRTAHDRNDLVLGSTWRNARIGGGKGPAAQAAAVQTDLHADTPAVEHIDA
                     LGHVVARIDHNRRIDRATGLPVDEMLVTRSETDVEGNLRRVVDPRGIEIVVHGYDLVG
                     TRAVSTTADGGRRWRLSDALGKMAIAGDAKDNRIRIVYDSMQRPSEFRVRPFGLAERL
                     RERHVYGTAADVAANANGRLIRVSDGAGTVATPRYDFKGNLLETERRFLADPRLVPDW
                     SAPPALDPAAHVTLTRYDALNRLVELTAPDGSVETYGYNETNLVETVEVAVAGGAVVP
                     YVANIDYDAKGQRRLIVYGNGVETDYDHDPETDRVARIRTRRTADGAVLQDLAYTYDP
                     VGNVTAIADGALPVHYFNNVVVDPGGAFTYEAVYRLASATGREHAAANQPPDWSDAHR
                     MSLPHKTDGNALQRYEQWFEYDRSGNLETIRHRAGRAALANAWTRTLQYGALNNRLQS
                     ALVGAGPAETPAYDLHGNTVALAHLASLDWDEDNRLTHVVNNAGDRIWYGYDGEGNRV
                     RKLVVHPDGTSQDRIYIGRWERVRNRRADGSLRVERETLHVLDGQQRIATVERRTAGV
                     DPGPASLVCYQFGNALGTALLELNDSAAILSYEEYHPFGTTALQSASTQRRNVPKRYR
                     FTGKERDTESGLYYHGARYYAPWLCRWTAPDPAGAADGQNLYAYAGNRPIGSGDPTGM
                     WEMPSWRTVAIVTAVVVVGAVVTVATAGAAGPVIAGAVASVGLTGTAATVATGVAVGA
                     VSGAVAGAASGAAGELTRQTVNSRALGLGNQEFSGRAILREAGSGAVTGAAVGAAVGG
                     AAAFA"
BASE COUNT         1389 a         3653 c         3458 g         1385 t
ORIGIN      
        1 agcacggccc ggctcgggac cgaggtccag acccggctgt ggccgcgcgt ccccgttggc
       61 acgcgccccg cgccgccggc gccgatgagc ttcgccctgc ctgcccgcca ggcctcgctg
      121 ctgctctcgc acagtgccca gccgcgcccc tgggcgacgg tgtcggtgcg cgcggccgtg
      181 ccgctgaccg cgccggcctt cgccggctat cgggtcggcc gcgaggtctc cttcctcgag
      241 cgcaagcggc ccggcgcggt cagcgccggc gacgtgctca aggttcggat caccgtcgag
      301 gcgccggtcg accggacctg ggtcgtggtc gaggacccgg ttcccgcggg agcctcggtg
      361 atcagcggcg gcggcggcca gtcggcgctg ctggcggccc gggcaagcgg cggcagcggc
      421 atctggccga gctatatcga gcgcggcaac gacgcctggc gcgcctatta cggctggctt
      481 ccgcaggggc gaacgacggt cgaatatgtg ctcaggatca acggcgccgg ccggttccag
      541 ctgcccccga cccgggtcga ggcgatgtat tcgccggaaa ttcacgcggc gctgcccaat
      601 aggccggtgg tggtgacgcc gtgaggagga ggaggcacca tcatcctccc catcggccat
      661 gatggggagg gggaccatgc gaagcatggt ggaggggtag ctggcgctgc cctccggcgg
      721 ttcgcgcgtg atgcttggaa ggcatcgctg gctacccctc caccaccctt cgggtggtcc
      781 ccctccccac gcgctccgtg cggggggagg atggctccgc cgccgcccgg tgctttgcct
      841 cctcctgccc ctcctcgtca tcggcgcgct cgccgcctgg acccgtccgc ccgacctgcc
      901 aagctacgag cgggcgaggg gggactggcg ggcgagcgag gcctggcttc gaggccgcga
      961 cgggcggctg cttcagacgg tgcggatcga tttcgccgtt cgccggctcg aatgggtgcc
     1021 gatcgagcgc atctcgccgt cggtgcgcga ggcggtggtc gcgtcggagg accggcattt
     1081 cgagcgccac ggcggcgtcg actggagcgc cttcgccggc tcggcctggg cggccctgcg
     1141 cgggcggccg gcgcgcgggg cgagcacgat cagcatgcag gtggccggct tcctcgcgcc
     1201 cgagctcggc gggcccggcg gccgctcgtt cgcgaacaag cttcgacagg cccgcgccgc
     1261 ccgcgccctc gagtcggagt ggaccaagcc gcagatcctc gaagcctatc tcaacctggc
     1321 caccttccgc ggcgagaccc agggcgtcgg cgccgccgcc cgcgccctgt tcggccgggc
     1381 gccggatgcg ctcggccgcg aggacgccct cctgctcgcc gccctgctgc cggcaccgca
     1441 ggccccgcct tcggcggtcg cgcggcgcgc gtgccggatc ggccgagtcg ccgattgcgc
     1501 gacgatgacg gcccgggccg aagcgatgac ggggcccgga cggcggctcg ccgacgatcc
     1561 cggcctcgct ccccatctcg cctatcgtct gctgaagaag cccgggcaga ccgccgtcac
     1621 cacgatcgac ccccaggtcc aggcgctggc cgcgggcgcg ctacgccgcc aactgatcgg
     1681 gctgggcggc actcgggcga gagacggcgc cgtgatcgtc atcgacaatg ccaccgccga
     1741 ggtgctcgcc tatgtcggcg gggtcggcgg cgcgtcgacg gctccggcgg tcgacaatgc
     1801 gagcgcgccg cggcaggcgg gatcgaccct caagccgttc ctctacggac aggcgatcga
     1861 gcgcggcttc atcaccgccg cctcgatcct cgacgacagc ccggtccagc tcgacaccgc
     1921 ctccggcctc tacgtgccga gggactatga caacagcttc aagggcccgg tcagcgtgcg
     1981 caccgctctg gccggctcgc tcaacgtgcc ggcggtgcgc gcgctgctgc tgaacggggt
     2041 cgaggagttt cgcgaccggc tctgggacat cggctatcgc ggcctcaccg aggacggcga
     2101 ctattacggc tacagcctcg cgctcggctc ggccgaggtg accctgatgc agcaggcggc
     2161 cgcctaccgc gcgctggccg acggcggccg ctaccgcccg ctccgcctcc gccccgacga
     2221 tccggccggc cgaggcaagg ccgtcgccga tcccgtcgcc gcctggatcg tcgccgacat
     2281 gatggcggac gccgacgccc gcgccgtcac cttcggaatc gattcggcgc ttcgactgcc
     2341 cttctgggcg gcggcgaaga ccggaacctc gaaggcgatg cgggacaatt ggtgcatcgg
     2401 cttttccgac cgctacacgg tcgccgtctg gatcggcaat tcggaggggg attcgatgag
     2461 ccgcgtttcc ggcaccagcg gcgcggcacc aatctggcgc gacgtcatgc tcgcgatcca
     2521 ccgcggccgg cccggccggg cgccgccccg tccggcgggg ctcgaggcga gggccgtcgt
     2581 cttcgccgac gggaccgagc cggcgcgacc cgaatatttc ctgcgcggga ccgcgcagtc
     2641 gagcatcgag ggcgcgcccg cggccgcccg ccgtccgcgc atcgccaatc cggtttccgg
     2701 agcggtctat gcgctggacc ctgatatccc gatggcgcgg cagcggatcc gcctgaccgc
     2761 gaccggcgcg gccggcggcg agcggatgct gctcgacggc cgggatgtcg gcgccgccca
     2821 ggacggcccg atggtgctgc cggcgccggg cgcccaccgc ctcgccctgg tcgacgagcg
     2881 cggcaggaca ctcgaccgca gcctcttctc ggtgcgctga ggcgaagggg cgagaataat
     2941 gcttgcgatc ggcccccacg ccttaccggg gagggcctcg tccaagctcg accttcggcc
     3001 gccgcacgca atccggcgga gggcgcgcgg ccgacttccg ggcgcaggca tgtgaatagg
     3061 ttcattatgg agcgcattga acttgaataa atccgaagac tgagttcatc tgtcgaggga
     3121 gcgacggatg gtcgggcagc gcgacgagag caagaaacca ccgcgcaacc gcatcgagat
     3181 tcccaagatc gagctgccca agggcggcgg cgcagtgcgc ggcatcgacg agaaattccg
     3241 ggtaaattcg gccaacggca cctcctcctt ctcgatcccg ctgccgttcc acccgggccg
     3301 ggacgcgttt tcgccgggcc tctcgatcgc ctacgattcg ggcagcggga tggggctttt
     3361 cgggctggga tggtcgctcg acctgccgag catctcgcgg tcgaccggca agcgcctgcc
     3421 ggcctatgac gacgcccgcg agagcgacga tttccagctg accggcttcg aggacctggt
     3481 tcccgtcctt cggccggccg ggggcggagc gctcgagccc gacgtcgagc agatcggcga
     3541 ccatcggatc catcgctacc gtccgcgcac cgagggcgcc ttcgcccgga tcgagcgaat
     3601 ccatcgggcc ggggaggcgg cggcctattg gcgggtcatg ccgggtgacg gcactacgac
     3661 cgtcttcggc cggaccgcgg ccgcccggat cgccgatccc gaggccggcc atcgcgtctt
     3721 ccgctggctg cccgaatggc gcttcgacga tcgcggcaat tgcatcgaat atgtctacaa
     3781 gccggaggat ctggccaacg tccccaagct cgcctccgag gcccggcggc acgagggcga
     3841 agcggccttc gccaaccggc acatcaagcg catcctgtac ggcaaccggg tcccctacga
     3901 tcccggcgcg gccgctccgg cgcacgatcc gctgccgccg gcgggcctga accatttctt
     3961 ccaggcgctg ttcgactatg gcgagcatgg cgacggtctc gatccggcgg agacgaggga
     4021 ctggatggcg cgcggcgatc cctggtccga ccgccgtccc ggcttcgaca tgcgaagcta
     4081 caggctctgc cggcggatcc tgttcctcaa ccgcttcgag gagctcgacc cgctcgccgc
     4141 cgagccgccc tgggtggcgg taaagggcct cgagttcacc tatgtcggag cggacttcgc
     4201 gacccccgcc gcgccgccga tcgaatgcga gcggctgacg gcggtcgccg aggcgggcta
     4261 tcggcccgac ggcgccggcg gcatcggccg caccgccctg gcgccgatgc gcttcgacta
     4321 tcatccggcc gcgatcgaca ccgcggtccg gcgcacgccg cgcaaggatc tgcccaacgc
     4381 gcccgacggc ctgatgggcg aggtccatgc cgtcgacctc tacggcgacg gagtgccggg
     4441 cctgctgtcc gagcgcgagg ccgagtggct ctacgcgcgc aacctgggcg gcggacgctt
     4501 cggcaatgcg gtgccggtgt gggagaagcc gtccttcgcc gggatcgcct ccggggtgct
     4561 gcaggtccgc gatctcgagg ccgacgggtc gaaggagatc gtgatcgagc agccgggcct
     4621 cgccggctat ttcccgctcg acgagggcgg ccgctacggc ccgatggtcg cgttcgcgcg
     4681 gcccgcggtg gtgccgacgc cggcggctcg ccggcggctg atcgatctcg acggcgacgg
     4741 ccggcccgag atcgtgatcg ccggggacag cgagctgcac tacctccaaa gccgcggccg
     4801 gcgaggctac ggacgctggt gcagcgcgcc ttatccgccc ggcgaaggcc cgcggcggct
     4861 gctgatcgcc gacgaggagg aaggaatcca cctcgcggac atgaccgggg acgggctcag
     4921 cgacctcgtc cgggtccgca gcgattcggt cgcctattgg cccaatctcg gcttcgggcg
     4981 cttcggtccg ttggtcgaga tggagcgatg cccggtcctc gattcgcccg agcggttcga
     5041 tccgcgccgg gtgcagttcg ccgatctcgg cggcacgggc gcggccgatc tcgtctacga
     5101 ttccgacttg ggcccggtcg cggcagtcaa cctatgcggc aacggctggg ggccgaccct
     5161 gcgctttcca ggcctcgccg gcccgcagac ggcgacgcgt ctcgccgtca tggacctgct
     5221 cgcgcagggg acgccctgcc tggtgcggtc gagcagcctc ccggcggatg ccggcgcgcc
     5281 gctcgcctgg atcgacctgt atcacggccg caagccctat ctgctcgaga cggtggacaa
     5341 tggcgccggc aagaccgtcg ccattcgcta ccgcagctcg gtgcagttcc agctcgagga
     5401 cgaggccgcc ggccggccct gggtcacgcg gctgtccttc cccgtcgcct gcgtcgcccg
     5461 gaccgagacc cgcgactcgg tcaccaacag cctgttcgtc acgacctatc gctatcgcca
     5521 cggctattac gaccaccgcg accgggaatt ccgcggcttc gccttcgtcg aggagatcga
     5581 cagcgaggat tattcggcgc tggccggagc cgaggccggc aacgcgcccg cgccggagct
     5641 tcacgagccg ccgacgcgca cccgccgctg gttccacacc ggcgcctttc ccggctggag
     5701 cctgagccag cgcctcgctg cggagttcgc gccgcttccg gccgccgccg cgggcctcga
     5761 ggggggcgtg agcgaggagc ttccgcccgg cggcccgggc gaccggatcg cctcgcaccg
     5821 ggtcctcaag tcaaagccgc tgcgcaccga ggtctgcgtt ctcgacggca cggccgagga
     5881 agacctgccc taccggatca ccacgcacgg cttctggatc cggctcgtcc agcctccgga
     5941 cgggaatcgg tacggcgtcc accaggcgat cgagtccgag acggtcgaga tccacatgga
     6001 gcgcgactcc gccgacccgc gcatctcgca tcgcctgacg ctcgccgtcg acgaccacgg
     6061 cctgccggag cggtcggccg agatcgccta tggccgggcc gcgccgccgg ccggactcga
     6121 cgcccagatc gccgcggcgc agcagcgctc gcatgtgacc gtccagatcg tgcgccgcac
     6181 gcccgacgtc gaccagccgg cattccggcg gcttcggacg ccgttcgaaa ccgcctcgtt
     6241 cgagctgacc ggcgcggcgc ccgccgggac ggcgtggacc gcctccgccc tcgacgccga
     6301 cttcctgggc gcggcgcccc gcgactatgc ctcgccggtc ccggccggcc ccgcccgccg
     6361 gctgatcgag ctcgagcgga tcgtcttcgc ggacgatgcc gatcccaacc tgccgctcgc
     6421 cttcggcacg atgggcgcaa ggggcatcgt ccaccagagc tatcgcctcg ccttcgattc
     6481 gaccctcgtc caggcgctct acgggaccga cgtcgccccc gccgacctca gctccgccgc
     6541 ctatcttcgc gaatcggcgc tggtcgccgc cgggctgttc ccgccgggcg aggccggacg
     6601 ctggtggacc gcttcgggcc atgtcgaata cgggcccaac ccccaagcgg ccttcttccg
     6661 gcccacggcc tatgtggacc cggtcgggca gcgcaccgag gtgcgcgaat atcgcgatta
     6721 tttcctgctc atcgaccgga tccgcgacgc cgcgggcaac gacgtcacgg tcacggcctt
     6781 cgactggttc gtcgtcgcgc cgagcgcggt acgcgacatc aatggcaata tctcggcggt
     6841 cgcctacgac cggctcgggc tcgtcgccgg gtcggcgctg atgggcaagg gcgccgaggg
     6901 ggacacgctg gccggcttcg acccgaaccc gagcgacgcc gccgtcgacg ccttcttcgc
     6961 ggacccggtc ggcgaggcgc gggccctgct cggagcggcg accacgcggg tcgtcatcga
     7021 tcatcgccac cggcccaatt ggtcggcggt gatcgggcgg gcgatgcacg aggcggacga
     7081 agccgcggcc ggcgttctcg ggcagctcca gatcgcgttc gactatagcg acggcttcgg
     7141 ccgggtggcc ctgcgcaagc tgctcgccga gcccggcgag gcgctggtga tggtcgacac
     7201 tgcggacggc cccaaggcga tctcggtgga cagcggcgcc gcgccccgct gggtcggaag
     7261 cggacgcagc gtcgccaaca acaagggcga cgtcgtcctt gccttcaatc cctatttcgc
     7321 gctcttccac ggctatgacg acgccgacgc gctggtcgcc gcgggccatt cgaagcgcag
     7381 ccattacgac gcgttcggcc ggctggtcga ggtcgagcat cccgacggca gccgcgagct
     7441 gagcgagatc gccgcctggt cgcgaaccgc ccacgaccgc aacgacctgg tcctcggctc
     7501 gacctggcgc aacgcccgga tcgggggcgg gaaggggccc gccgcccagg cggccgcggt
     7561 ccagaccgat cttcacgccg acactccggc cgtcgagcat atcgatgcgc tcggccacgt
     7621 cgtggcgcgg atcgaccaca accgccggat cgaccgggcg accggcctcc cggtcgacga
     7681 gatgctcgtc acgcgcagcg agaccgacgt cgagggcaat ctgcgccggg tcgtcgatcc
     7741 gcgcgggatc gagatcgtgg ttcatggcta cgacctggtg ggcacgcgcg cggtctccac
     7801 caccgcggac ggcggcaggc gctggcggct gtccgacgcg ctcggcaaga tggcgatcgc
     7861 cggcgacgcc aaggacaacc ggatccggat cgtctacgat tcgatgcagc ggccgagcga
     7921 gttccgggtg cggccgttcg gcctcgccga gcggcttcgc gagcgccacg tctacggcac
     7981 cgccgccgac gtcgcggcca atgccaacgg gcgcctgata cgggtgtccg acggggccgg
     8041 gacggtcgcg accccgcgct acgacttcaa gggcaacctg ctcgagaccg agcggcgctt
     8101 cctcgccgat ccgaggctgg tccccgactg gagcgcgccg cccgcgctcg acccggcggc
     8161 ccatgtcacg ctcacccgct acgacgcgct caaccggctc gtcgagctga ccgcgcccga
     8221 cggcagcgtc gagacctacg gctataacga gaccaacctg gtcgagacgg tcgaggtggc
     8281 ggtggcgggg ggcgcggtcg tgccctatgt cgccaacatc gactacgacg ccaagggcca
     8341 gcgccgactc atcgtctacg gcaacggcgt cgagaccgac tacgatcatg atccggagac
     8401 ggaccgagtc gcccggatcc ggacgcggcg gaccgccgac ggggcggtgc tccaggacct
     8461 cgcctatacc tacgacccgg tcggcaacgt caccgcgatc gccgacggcg ctctgccggt
     8521 ccattatttc aacaacgtcg tggtcgatcc cggcggcgcc ttcacctacg aggccgtcta
     8581 ccgcctcgcc agcgcgaccg ggcgcgagca tgcggccgcc aaccagcctc ccgactggtc
     8641 cgacgcccac aggatgtcgc ttccccacaa gaccgacggc aacgcgctcc agcgctacga
     8701 gcagtggttc gaatatgacc ggtcgggcaa tctcgagacg atccggcatc gcgccggccg
     8761 cgccgcgctc gccaacgcct ggacccggac ccttcaatat ggcgctttga acaaccgcct
     8821 gcagagcgcg ctcgtcggcg cgggacccgc cgagaccccg gcctacgacc tccacggcaa
     8881 caccgtcgcc ctggcccatc tcgccagcct cgactgggac gaggacaacc ggctgaccca
     8941 cgtcgtcaac aatgccggcg accgcatctg gtacggctat gacggcgagg gcaaccgcgt
     9001 ccgcaagctc gtcgtccacc ccgacggaac cagccaggac cggatctaca tcggccgctg
     9061 ggagcgcgtc cgcaatcgcc gggccgacgg ctcgctccgg gtggagcgcg agaccctcca
     9121 cgtgctcgac ggccagcagc gcatcgcgac cgtcgagcgg cgcacggccg gcgtggaccc
     9181 cggcccggcc tcgctggtct gctatcagtt cggcaacgcc ctcggcaccg ccttgctcga
     9241 gctcaacgat tccgccgcga tcctctccta cgaggaatat catccgttcg ggacgacggc
     9301 gcttcagtcg gcgagcacgc agcgccgcaa cgtccccaag cgctaccgct tcaccggcaa
     9361 ggaacgcgat accgagagcg gcctctatta tcacggcgcg cgctactacg cgccctggct
     9421 gtgccgatgg accgcgccgg atccggccgg cgccgccgac ggccagaacc tctacgccta
     9481 tgccggaaac cggccgatcg gatcgggcga cccgaccggg atgtgggaga tgccgagctg
     9541 gcggaccgtg gccatcgtca ccgcggtggt ggtggtcgga gccgtggtca cggtcgcgac
     9601 cgcgggggcg gcggggccgg tcatcgccgg cgcggtggcg agcgtcgggc tgaccgggac
     9661 ggcggccacg gtcgcgaccg gagtcgcggt cggagcggtc tcgggcgccg tcgcgggagc
     9721 ggcgtcgggc gccgccggcg agctcacccg ccagaccgtc aacagccggg ctctggggct
     9781 cggcaaccag gaattctccg gccgggcgat ccttcgcgag gcgggctcgg gcgcggtcac
     9841 cggagcggcg gtcggcgcgg cggtcggcgg agcggcggcg ttcgc
//
LOCUS       DASVQZ010000296         5686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_448041, whole genome shotgun sequence.
ACCESSION   DASVQZ010000296 DASVQZ010000000
VERSION     DASVQZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448041"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VF547_08085"
     CDS             complement(<1..475)
                     /locus_tag="VF547_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262817.1"
                     /translation="MSRLVRSLASHMLLAASRLLPPDLSSWSRAMVRELAEIDEDASA
                     LRFAAGCLRAAAALAIAARLRAIATAAPSRLSPLATPTWSLSPMYDILKRPRSLGLLC
                     AAIAVVSGLAYMYAGGAPTRYLVMNLAALVLGATAWLALGRTAGSRLAGAGAATLA"
     gene            complement(472..789)
                     /locus_tag="VF547_08090"
     CDS             complement(472..789)
                     /locus_tag="VF547_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8262818.1"
                     /translation="MTRKRALSSHARAMLGALVAAGPRWSYGYELMSRTGIRSGTLYP
                     LLMRLEAQGYLEAQWQPTAAGRPPRHAYRLTAAGRALAAAEAAVTPGSVSPARGRAGE
                     AAS"
     gene            complement(1014..2495)
                     /locus_tag="VF547_08095"
     CDS             complement(1014..2495)
                     /locus_tag="VF547_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8262819.1"
                     /translation="MKTITALSCGLMLGGCGPNLYSVPFYTSTALHNGPALKFLKEGG
                     PADVGGPDPTILVKDPYADGNMVALSASGGGMRASAFTLGVLAELDEVTGDGQSETAF
                     QKIDLISSISGGSWAVAAVLADRALGSTEPLAKRMTEIESRYAELAGAKVDRWAGRFI
                     PAVTLGLTYSQVYRRGASRPLPFAYFNSTLYPSHSPFVFTPAYLAHYKVKALGDPAEP
                     RRILLRSYALADIPIGYAASASGAVPGFTSAFADTALCQDRLPSYCLPSLGKKVRDKL
                     QLMDGGLYDNIGFKTALEVGLRDRDRIREIPATIIMIDSADVEDFQTMPDKGKDGGHI
                     VGLAKAGSFPNQNATFDRLRGPGFAAAGFDRRILLDFATAAGFDPDLHRRHLDDLPEL
                     AYYAAHDVGCYGADGRFIPGRRRLKAPADPGDPGKNLDILKGKGADCVSLNFARTGYL
                     HKTTFKYDRYAFRLRYQLGRLAVRMKRDSIAEALFRTTRTPPP"
     gene            complement(2634..3731)
                     /gene="lptG"
                     /locus_tag="VF547_08100"
     CDS             complement(2634..3731)
                     /gene="lptG"
                     /locus_tag="VF547_08100"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX8262820.1"
                     /translation="MINVNFFPSKRIAFYMARMFVLRSLAVLAALVLVLMTLDLLGEA
                     GDILEAPGNGDAELWRYVSLRIPQLISRFLPFAVLLGTLITLATLNQNSEVISMKAAG
                     ISAHQIIAPLVVASLGIAAVSFVFNERVVTRATETLTSWEKVDYGPVPRDSGIRPNVW
                     VRWGDDLVMAREVRGEGEATELRSLSIYDRQHETLKTIVTARRGVRHGDGWRLEGVET
                     FDVASGATTRTQSIDYGKGLDPSQFTLARVSADEQDFVTLRRSVKQLKLAGRPTAELE
                     SGLWHKLSGPLSAVLMPLLAGVAAFGLARSGQLLLRAILGMALGFAYFVADNFALAMG
                     NLGAYPPLLAAWAPFVLFALIGETVLIRTEE"
     gene            complement(3728..4951)
                     /gene="lptF"
                     /locus_tag="VF547_08105"
     CDS             complement(3728..4951)
                     /gene="lptF"
                     /locus_tag="VF547_08105"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX8262821.1"
                     /translation="MSPFTQIDRYIARLILAPLLATLALASMLLVLDKMLRLFDFVVT
                     EGGPVGVVWRMLANLVPEYLALGIPLGLLLGTLLAFRKLALSTELDSLKAVGLSYTRL
                     LRVPYVFAVVLMALNVAIVGFVQPVARYGYQQLRFDLRSGALGASIKVGEFTNLGKRM
                     TLRVEASQDQGRLLHGVFVRAETRDGRTIAVTADHGTFLATDDPDVILLRLSNGRLVH
                     NSPRYKAPRVLSFRIQDLPVDLPAVARFRGRGRDEEQEMTIPELFRFGAAAAVPPATR
                     NAVRANFHYRVTEIVMMLLMPLLAVALAVPPKRSTSGLGIFLAIIMVVAYHKVNQYAE
                     EMAALGRVDPLIALWTPFLLFAALIMWMYWTLAYKPGGQPIGGLERAFSKLGKGFGRL
                     LRLGSGGRREAVPAE"
     gene            5137..>5686
                     /locus_tag="VF547_08110"
     CDS             5137..>5686
                     /locus_tag="VF547_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262822.1"
                     /translation="MASSEVPHDALADGDGAMTAAAGESAGTGRSRDRVLSPDDELVF
                     PLSKGAFAQYREDPETPRHLRIFYRDKEIALADPAFFAFGEALVGQSRFRAGDAVRWA
                     DLEWPRVRQLLEQLIAARVLRHAQEADESAGPLRHEDRPSPLRPAPCARPRTWDESEE
                     VMRLITGRPLELGHLELVVPVFR"
BASE COUNT          978 a         1871 c         2009 g          828 t
ORIGIN      
        1 gtgcgagcgt cgccgctccg gcgccggcaa ggcgcgagcc cgcggtccgc ccgagcgcga
       61 gccatgcggt cgctcccagc accaaagcgg caaggttcat cacgagatat cgggtcggcg
      121 cgccgcccgc gtacatatag gccagccccg acaccacggc gatggcagcg cagagaaggc
      181 cgagcgatcg aggccgcttg aggatgtcgt acatggggga aaggctccag gtcggggttg
      241 cgagcgggga caggcgggag ggcgcggcgg tggctatggc ccgaagccgg gcggcgatcg
      301 ccagagcggc cgcggcgcgc aggcacccgg cggcgaagcg aagcgccgag gcgtcctcgt
      361 cgatttcggc cagctcgcgg accatggcgc gcgaccagga cgacagatcc ggcgggagca
      421 ggcgcgaagc ggcgagtaac atgtgcgagg cgaggctccg aacgaggcgg ctcacgacgc
      481 cgcctcgccg gctcggccgc gggctggcga aacgctcccg ggcgtcaccg ccgcttccgc
      541 ggctgcgagc gcccggccgg cggcggtcag ccggtaggca tggcgcggcg gccggccggc
      601 cgccgtcggc tgccactggg cctcgagata gccttgcgct tcgagccgca tgagaagggg
      661 gtagagcgtc ccggacctga tcccggtgcg cgacatcagc tcgtagccgt agctccagcg
      721 cggcccggcc gcgaccagcg cgccgagcat ggcgcgggca tggctggaca gggcgcgctt
      781 gcgggtcatc gcggcataat ctacttatgt agacttaagt caagccggta tcggctgagg
      841 gccgacgctt ccatagcgct tgccgttcgg gctgagcctg tcgaagccct gccttcccct
      901 acaggaacga gaaggaaagc ccttcgacaa gctcagggcg aacgggcgac atgattcaaa
      961 cctgatcccc aatggctcca ggagccctcg cgatggtgag gctcgacccc ggcttagggc
     1021 ggcggggtgc gcgtcgttcg gaacagggcc tcggcgatcg agtcccgctt catgcgcacc
     1081 gccagcctcc ccagctggta gcgaagccgg aaggcatagc gatcgtattt gaaggtcgtc
     1141 ttgtgcaggt agccggttct cgcaaagttg agcgagacgc agtccgcgcc ctttcctttc
     1201 aggatgtcca aattcttgcc ggggtcgccc ggatcggccg gcgctttcag ccggcgccgg
     1261 ccggggatga agcgaccgtc ggctccgtag cagccgacgt cgtgcgccgc gtaataagcg
     1321 agctcgggca ggtcatccag gtggcggcgg tggagatcgg gatcgaaacc ggcggcggtc
     1381 gcgaagtcca gcaggatgcg ccggtcgaaa ccggcagccg cgaagccggg gccgcggagc
     1441 cggtcgaaag tggcgttctg attggggaag ctgcccgcct tggcaaggcc gacgatgtgg
     1501 ccgccgtcct tgcccttgtc cggcatggtc tggaaatcct cgacgtcggc cgagtcgatc
     1561 atgatgatcg tcgccgggat ttcccggatt cgatcgcggt ccctcagccc gacttcgagc
     1621 gcggtcttga agccgatatt gtcgtagagc ccgccatcca tcagctggag cttgtcgcga
     1681 acctttttcc cgagcgacgg caagcaatag ctcggcagcc ggtcctggca gagggcggtg
     1741 tcggcgaagg cgctcgtgaa gcccggaacc gcgccgctcg ccgatgcggc gtagccgatc
     1801 ggaatgtccg ccagagcata ggatcgaaga aggatccggc gcggttcggc gggatcgccc
     1861 agcgccttca ccttgtaatg agcaaggtag gccggcgtga acacgaaggg cgaatgggac
     1921 gggtacagcg tcgaattgaa ataggcgaaa ggcagcggcc tggacgcgcc gcggcgatag
     1981 acctgcgagt aggtcaggcc gagcgtcacc gccgggatga accggccggc ccagcgatcg
     2041 accttcgcac cggccagctc ggcgtagcgc gactcgatct cggtcatccg ctttgcgagc
     2101 ggctcggtcg aaccgagcgc ccggtcggcc agaacggcgg cgacggccca cgacccgccc
     2161 gagatcgagc tgataaggtc gattttctgg aaggcggttt ccgactgtcc gtccccagtc
     2221 acttcgtcga gctcggccag gacccccagg gtgaacgcgc tcgcccgcat gcctccgccg
     2281 gacgcggaga gcgccaccat gttgccgtcg gcataagggt ccttgaccag gatcgtgggg
     2341 tcgggcccgc ccacgtcggc aggtccgccc tctttcagga atttcagagc gggcccgttg
     2401 tgcagggccg tcgacgtata gaatgggacg gaatagagat tggggccgca gccgcccagc
     2461 atgaggccgc aggacaaagc cgtaatcgtc ttcattcctg accccctacc cctatcgccg
     2521 acagtgaagc ggagcaggga cattggcaag ccggtcaact tggagattta ttggccgtcc
     2581 ggcacgcctg cccggcgatg accccgggcg ccgtcgctgc gcccggccac cccctattcc
     2641 tccgtccgga tcagcaccgt ctcgccgatc aaggcgaaga ggacgaaggg cgcccaggcg
     2701 gcgagcagcg gcgggtaggc gccgagattg cccatggcca aggcgaaatt gtcggcgacg
     2761 aaataggcga agcccagcgc catgcccagg atggcgcgca ggagcagctg gccggaacgg
     2821 gccaggccga aggcggcgac tccggcgagc aagggcatca gcaccgccga caaggggccg
     2881 gacaatttgt gccagaggcc ggattcgagc tcggcggtcg gccggccggc gagcttgagc
     2941 tgcttcaccg aacggcgcag ggtcacgaaa tcctgctcgt cggcggagac gcgggccagc
     3001 gtgaactggc tcggatcgag gcccttgccg taatcgatgg actgggtccg ggtcgtcgcc
     3061 cccgaggcga cgtcgaaggt ctcgaccccc tcgagacgcc agccgtcacc gtggcggacc
     3121 ccgcgccggg cggtgacgat cgtcttgagc gtctcatgct gacggtcata gatgctgagg
     3181 ctgcgcagct cggtcgcctc gccctcgccg cgcacctccc gggccatgac aaggtcgtcg
     3241 ccccagcgca cccagacgtt cggccggatg ccgctgtcgc gcggcaccgg cccgtaatcg
     3301 accttctccc agctcgtcag ggtctcggtg gcgcgggtga cgactcgttc gttgaagacg
     3361 aagctgaccg ccgcgatgcc gaggcttgcg accaccagcg gcgcgatgat ctgatgggcc
     3421 gagatgccgg ccgccttcat gctgatcacc tcgctgttct ggttgagggt cgcgagcgtt
     3481 atcagcgtgc cgaggagcac ggcgaacggc aggaagcggg agatgagctg gggaatgcgc
     3541 aggctgacgt aacgccacag ctcggcgtcg ccgttgccgg gcgcctcgag gatgtcgccg
     3601 gcctcgccga gaaggtcgag ggtcatcaga accagcacca gcgcggccag caccgccagc
     3661 gagcgcagga cgaacatccg cgccatgtag aaggcgattc gcttcgacgg gaagaagttg
     3721 acgttgatca ttcggccggg accgcctcgc ggcgtccgcc gctgccgagc cgaagcagcc
     3781 ggccgaagcc cttgccgagc ttcgagaaag cgcgctcgag cccgccgatc ggctgcccgc
     3841 ccggcttgta ggccagggtc cagtacatcc acattatcag ggccgcgaag aggaggaacg
     3901 gggtccacag ggcgatgagc ggatcgaccc ggcccagcgc cgccatctcc tccgcatatt
     3961 ggttcacctt gtggtacgcg acgaccatga tgatcgccag gaagatgccg aggcccgacg
     4021 tgctccgctt gggcgggacc gcgagcgcga ccgccagcag cggcatcagc agcatcatca
     4081 cgatctcggt cacccgatag tggaaattgg ccctcaccgc gttgcgggtc gccgggggca
     4141 ccgccgccgc cgcgccgaag cggaacagct cggggatggt catctcctgc tcctcgtcgc
     4201 ggccgcggcc gcggaagcgg gcgacggccg gcaggtcgac cggaaggtcc tggatccgga
     4261 agctcagcac cctcggcgcc ttgtagcgcg gcgaattgtg gacgaggcgg ccgttcgaca
     4321 gccgcagcag gatgacgtcg ggatcgtcgg tggcgaggaa ggtgccgtga tccgccgtga
     4381 ccgcgatcgt tcggccgtcg cgcgtctcgg cgcggacgaa cacgccgtgg agcagccgcc
     4441 cctggtcctg gctcgcctcg accctcagcg tcatcctctt gccgagattg gtgaactcgc
     4501 cgaccttgat cgacgccccc agcgctcccg accgcaggtc gaagcgcaat tgctgatagc
     4561 cgtagcgcgc caccggctgg acgaagccga cgatggccac gttgagcgcc atcagcacga
     4621 ccgcgaacac gtagggcaca cgaagcagcc gggtatagct caggcccacc gccttgaggc
     4681 tgtcgagctc ggtcgacaag gcgagcttgc ggaaggcgag cagggtgccg agcagtagcc
     4741 cgagcggaat tccgagcgcc agatattcag gcacgagatt ggcgagcatc cgccacacca
     4801 ctccgaccgg gccgccctcg gtgacgacga agtcgaacag gcgcagcatc ttgtcgagca
     4861 cgagcagcat cgaggcgagc gccagggtcg ccagcagcgg cgcgaggatc aggcgcgcga
     4921 tgtagcggtc gatctgggtg aacgggctca ccggccgatc aaagaggcgg tttgatccgc
     4981 ttttcaagaa ttatccgccg ccccggcgac gtttcggcgg ccagcccggg gtccgcgccg
     5041 ggcaggttca tggcgttcaa gacgcatccg accccatgcg gtagcgacaa gcgccggccg
     5101 cgctccgccg gcaaaggacg ggggtcggaa gaatccatgg cttccagtga agtcccgcac
     5161 gatgccctgg ccgacggtga cggcgccatg actgccgccg ccggcgagtc cgccgggaca
     5221 ggtcggtcgc gggatcgggt gctaagtccc gacgacgagc tcgtctttcc cctctccaaa
     5281 ggagcgttcg cgcaatatcg ggaggatccg gagacgccgc gccacctgcg gatattctat
     5341 cgcgacaagg agatagcgct cgccgatccg gctttcttcg ccttcggcga ggcgctggta
     5401 ggccaatcgc ggttccgcgc cggcgatgcc gtccgatggg cggatctcga atggccccga
     5461 gtacgccagc tgctcgagca gctgatcgcg gcgcgggtgc tgcggcatgc gcaggaggcg
     5521 gacgagtcgg cggggccgct tcgccacgag gaccggcctt ccccgctcag gccggcgccc
     5581 tgcgcccggc cccgcacctg ggacgagagc gaagaggtga tgcgcctgat caccggccgg
     5641 ccgctcgagc tcggccatct ggagctggtc gtgccggtgt tccggg
//
LOCUS       DASVQZ010000297         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_448526, whole genome shotgun sequence.
ACCESSION   DASVQZ010000297 DASVQZ010000000
VERSION     DASVQZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448526"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VF547_08115"
     CDS             complement(<1..191)
                     /locus_tag="VF547_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262823.1"
                     /translation="MSAQHDFYTARAAEARADADKATLDNVRDRCLRSAAAWEAMAAR
                     AHRSENHRARLEADKAAAAA"
     gene            complement(269..475)
                     /locus_tag="VF547_08120"
     CDS             complement(269..475)
                     /locus_tag="VF547_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8262824.1"
                     /translation="MTIGTVKFFNADKGYGFIQPDGGGNDAFVHISAVQAAGMQTLDR
                     DQRVSYELETDRRGKTSAVNLQSA"
     gene            769..1728
                     /locus_tag="VF547_08125"
     CDS             769..1728
                     /locus_tag="VF547_08125"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX8262825.1"
                     /translation="MTLPPALRRLRLPAIGAPLFIVSNPDLVIAQCKAGIVGAFPALN
                     ARPQSLLDEWLHRIDEELAAWDRDHPDTPAAPYAVNQIVHRSNDRLQQDMATCAKWRV
                     PIVITSLGAIVEVNEAVHGWGGITLHDIINDRFARKAVEKGADGVIAVAAGAGGHAGT
                     LSPFALVQEIRQWFEGPLILSGAIATGRALLAAQAMGADLGYIGSPFIATAEANADAA
                     YKQGICDGRAGDIVYSSFFTGVHGNYLRASILASGLDPDNLPEGDLKTMDFGGGSSAK
                     KAWRDIWGSGQGIGAIDRVRPAGDYVAQLIREYGEAKAELCAR"
     gene            complement(1861..>2280)
                     /locus_tag="VF547_08130"
     CDS             complement(1861..>2280)
                     /locus_tag="VF547_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX8262826.1"
                     /translation="DSLYTEAMLLADEARGYFDRQGREERMMLDPLLRVGFSCESLKV
                     TTRLMHVIAWLLTQRAVEAGELSRTQARRPARRLGEAPDSDEGLLPRLPANALALVNA
                     SRDLYARVKRLDEAGGLGPEPQASPARSLMSRLERSL"
BASE COUNT          330 a          772 c          811 g          367 t
ORIGIN      
        1 gccgcggcgg cggccttgtc ggcttcgagg cgggcgcggt gattctcgct gcggtgagcc
       61 cgggcggcca tcgcttccca ggcggcggcg gagcgcaggc agcggtcgcg gacattgtcg
      121 agggtggcct tgtcggcgtc ggcgcgggcc tcggcggccc gggcggtgta gaaatcatgc
      181 tgggcggaca tagggtcttt ctgctctccc tcgcggaagg atggggacac gtactttttt
      241 cgcgaaaagg tacgtgtccc cttttgcgtt aggccgactg caggttgacg gcgctggtct
      301 tgccgcggcg gtcggtctcg agctcgtagg agacgcgctg gtcgcggtcc agggtctgca
      361 ttccagcggc ctggacggcg ctgatgtgga cgaaggcgtc gttgccgccg ccgtcgggct
      421 ggatgaagcc atagcccttg tcggcgttga agaacttcac ggttccgatg gtcatttcac
      481 ttttcctttt ccgatggcgg gtcgtcgatc ggcccgccgg tgctgcgaga ggcgagagaa
      541 ggaaaaagga gccgcaaagc gccaaagacc gttcatttgc gactggtagc ggcactccat
      601 gtaggggccg gggccgaaaa tagcaaattc gcccccgccg cgccgcggcc gtcgaacggc
      661 gggcgcggct tgccgaggcg gagccgctgc cgctcggggc cgccccgcgc tcggccccga
      721 cgggggactc gtccttgcgc cggacgcttc ttccgttaag gcggcgcgat gaccctgccc
      781 ccggccctgc ggcgcctgcg cctgcccgcg atcggcgccc cgctcttcat cgtctccaac
      841 ccggatctgg tcatcgccca gtgcaaggcc gggatcgtcg gcgccttccc ggcgctcaac
      901 gcgcggcccc agagcctgct cgacgaatgg ctgcatcgca tcgacgagga gctcgccgcc
      961 tgggaccgcg atcatcccga cacgcccgcc gcgccctacg ccgtcaacca gatcgtccac
     1021 cgatccaacg accggctcca gcaggacatg gcgacctgcg ccaaatggag ggtgccgatc
     1081 gtgatcacct cgctcggcgc gatcgtcgag gtcaatgagg cggtgcacgg ctggggcggg
     1141 atcaccctcc acgacatcat caacgaccgc ttcgccagga aggcggtgga gaaaggcgcg
     1201 gacggcgtga tcgcggtcgc cgccggcgcg ggcggccatg ccgggaccct gtcgcccttc
     1261 gccctcgtcc aggagatacg ccagtggttc gagggaccgc tgatcctgtc gggggcgatc
     1321 gccaccgggc gcgccctgct cgccgcgcag gcgatgggcg ccgatctcgg ctacataggc
     1381 tccccgttca tcgccaccgc cgaggccaat gccgacgccg cctacaagca gggcatttgc
     1441 gacgggcggg cgggcgacat cgtctattcg agcttcttca ccggcgtcca cggcaattat
     1501 ctgcgcgcct cgatcctcgc ttcgggcctc gatcccgaca acctgcccga gggcgatctg
     1561 aagaccatgg acttcggcgg cggcagcagc gccaagaagg cctggcggga catctggggc
     1621 tcgggccagg ggatcggcgc gatcgaccgg gtccggccgg cgggcgacta tgtggcgcag
     1681 ctgatccgcg aatatgggga agccaaggcc gagctctgcg ctcgctgatt cttccccggc
     1741 attcgccggg gagggggacc gcgcgaagcg cggtggaggg gttcttcgga gtccgaaagg
     1801 cccctccacc aggcttcgcc tggtccccct ccccgagaaa tctcggggag gaattttctc
     1861 ttacaatgac cgctccaggc ggctcatcag gctgcgggcg gggctggcct ggggctcggg
     1921 gccgaggccg ccggcctcgt cgaggcgctt gacccgggcg tagaggtcgc ggctggcgtt
     1981 gacgagggcg agcgcgttcg cgggcagccg cggcagcagg ccctcgtcgc tgtccggcgc
     2041 ctcgccgagg cggcgggccg ggcggcgggc ctgggtccgg ctcagctcgc cggcctcgac
     2101 cgcgcgctgg gtcagaagcc aggcgatgac gtgcatcagc cgcgtcgtca ccttcagcga
     2161 ctcgcaggaa aagccgactc tcagcaacgg atcgagcatc atccgctctt cgcggccctg
     2221 ccgatcgaaa tagccgcgcg cttcgtcggc aagcagcatc gcctcggtgt agagcgagtc
//
LOCUS       DASVQZ010000298         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_62146, whole genome shotgun sequence.
ACCESSION   DASVQZ010000298 DASVQZ010000000
VERSION     DASVQZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62146"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..447)
                     /locus_tag="VF547_08135"
     CDS             complement(151..447)
                     /locus_tag="VF547_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262827.1"
                     /translation="MKMIIFAAALTLGSATALAQTGNTVDDPSQASGPRGVTQQGTDP
                     DGQACTPAGYNAGTSSYPTCEAGSAGAASTATPPRCSKTVKDHCVQAYERGVRR"
     gene            627..1037
                     /locus_tag="VF547_08140"
     CDS             627..1037
                     /locus_tag="VF547_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262828.1"
                     /translation="MTERTDTASEESGRLPSRRVVLALGAAAASTVVTIRPALAQTAG
                     SVLNCEIPVPAPQAGAGNFIAADGTIVPAGTAGAYPPLARPLKGEEVRAMLNGGRPPA
                     GVDSQAAQAYANYIRRLQAGMSGFTCYASLQMPR"
     gene            1055..1360
                     /locus_tag="VF547_08145"
     CDS             1055..1360
                     /locus_tag="VF547_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HEX8262829.1"
                     /translation="MAASLYAADPPETLRSVELEGLTILYHRRSGLTHILAPPAPQIL
                     AALAAGPATAADIVERIGADFELEADDPAAALAARLAELEAAGLVWRTPSPSLEGSS"
     gene            1266..1652
                     /locus_tag="VF547_08150"
     CDS             1266..1652
                     /locus_tag="VF547_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8262830.1"
                     /translation="MPPRPWRRDWRSWRRRAWCGGRHPPPWKGAARRGQVRAGKTLRG
                     FAPSAVRKVGASPQRTHPWPLPACREGKGRRVPPPWKGGVRGGSERTAFLQSLGWRVI
                     RFWNSEVSENPEGVAEAIIREVTATL"
     gene            1695..2555
                     /locus_tag="VF547_08155"
     CDS             1695..2555
                     /locus_tag="VF547_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HEX8262831.1"
                     /translation="MNHVFRLQVGPVGFRIGSQWRAPIATLRRLYAGYPEPDGLCHFT
                     VRLEAERPWRRWLRPSVAIRGDYVLPDAAPLPLAQGLLAAEMGMNLQMALGQKSFLLL
                     HAASVEKDGRALLMTGHSGAGKSTLAALLGERGWRFMGDEFALLGLDDGLLRPFPRAV
                     SLKNESLALFDGIDPGRLGPVLEGTPKGRIRHLRPNREAVARMAEPARPVLILFPRFG
                     RDLDPAVRPVGAAESFMRLTQASTNYVGLGERGFDALAALVGAVPSKAVDYADAAQAE
                     ALVDDLWRGA"
     gene            2552..>3435
                     /locus_tag="VF547_08160"
     CDS             2552..>3435
                     /locus_tag="VF547_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8262832.1"
                     /translation="MKDARLLVRLLADPARAREVADWTSLIAVARAESLAGSLAWRLE
                     GVDLPPPVEALLEDARRDSEAGRAQALWEAEMARRAIAPLGVPVILLKGSAYAAAGLE
                     ARRGRSIGDLDILVPRPALPDVERALLAAGWEWVKEDSYDDQYYRRWMHELPPLIHRE
                     RDRMIDVHHAILPLTARPTPDMDSMLAARVPLAEGLFVLSPPDMIVHAAAHLFADGEL
                     AGGLRNLWDIDRLVRQFSGQGYEFTVELRERAWEHDLTAPVARALRLARRLYGTPIDD
                     RLAGRSPVSDALYLRRLLA"
BASE COUNT          456 a         1282 c         1198 g          499 t
ORIGIN      
        1 ccctcgccgg gaaggaattc gatcggcggc ccatggcaga tcggggcgcg aaaggacaat
       61 ggggcccatc cgcgccgacg cggccccgcc cccggacgag ggcggagcca cccctgttca
      121 ccccgagccg ggctcggaga gagaatcagc ctagcggcgc acgccccgct cataggcctg
      181 gacgcaatgg tccttgaccg tcttcgagca gcgcggcggt gtggcggtgc tggcggcgcc
      241 ggcgcttccg gcctcgcagg tcggatagga gctggtgccg gcattgtagc cggccggcgt
      301 gcaggcctgg ccgtcagggt cggtgccctg ctgggtgact ccgcgcgggc cactcgcctg
      361 cgacggatcg tcgacggtat tgccggtctg cgcgagcgcg gttgcgctgc cgagcgtcag
      421 ggccgcggcg aagatgatca ttttcatgga aacagtcctc cttggacaca aaagcggccg
      481 cacctacctc ggcggcccga cctctaaacg aggccgaaac gttttttctc cgcgcgatcg
      541 tccacaagcg ggacagtcgg gctcggcccg tctcgccaat gggccgcttt acccttatga
      601 aagaggcttg gcgtaatgcg gatgcgatga ccgagcgaac cgacactgca agcgaggaaa
      661 gcggccggct gccgagccgc cgggtcgtgc tggcgctcgg cgccgccgcg gcctccaccg
      721 tcgtcacgat ccggcccgcc ctggcgcaga ccgccggctc ggtgctcaat tgcgagatcc
      781 cggtgcccgc gccgcaggcc ggggccggca atttcatcgc cgccgacggc acgatcgtcc
      841 ccgctggaac ggccggcgcc tatccgccgc tcgcccggcc gctgaagggc gaggaggtca
      901 gggcgatgct gaacggcggc cgcccgcccg ccggcgtcga cagccaggcc gcccaggctt
      961 acgccaacta tattcgccgt ctgcaggcgg ggatgagcgg cttcacctgc tacgcctcgc
     1021 tgcagatgcc gcgctgagcc gcggggcggc cggaatggcc gcatcgctct acgccgccga
     1081 tccgcccgaa acgctccgca gcgtcgagct cgaaggcctg accatcctct accatcgccg
     1141 ctccggcctc acccacatcc tcgccccgcc cgccccgcag atcctcgccg cgctcgccgc
     1201 cgggccggcg accgcggccg acatcgtcga gcggatcggc gcggacttcg agctcgaggc
     1261 cgacgatccc gccgcggccc tggcggcgcg actggcggag ctggaggcgg cgggcctggt
     1321 gtggcggacg ccatccccct ccctggaagg gagcagctag gcgtgggcag gttcgagccg
     1381 gcaagacgct tcgaggattc gcgccgtcgg ctgtccgcaa ggtcggcgcc tcgccccaac
     1441 gcacccaccc ctggcccctt cctgcgtgca gggaggggaa ggggagacgc gttccccctc
     1501 cctggaaggg aggcgtcagg ggcgggtcgg aacgcaccgc tttcctccaa tccctgggct
     1561 ggcgagtcat ccgattctgg aattccgagg tttccgagaa tccggaggga gtggccgagg
     1621 cgatcatccg ggaagtcact gcgactctct aacgcaccca cccctggccc ctccctgcgt
     1681 gcagggaggg gaagatgaac cacgtcttcc gcctgcaggt cgggccggtc ggcttccgga
     1741 tcggctcgca atggcgcgcg ccgatcgcga cgctgcgccg gctctacgcc ggctatcccg
     1801 agcccgacgg cctgtgccac ttcaccgtgc gcctcgaggc cgagcgcccc tggcggcgct
     1861 ggcttcggcc gtcggtggcg atccgcggcg attacgtgct gcccgacgcc gcgccgctgc
     1921 cgctggcgca gggcctgctc gccgccgaga tgggcatgaa cctgcagatg gcgctggggc
     1981 agaagagctt cctgctcctg cacgcggcca gcgtcgagaa ggacgggcgc gcgctcctga
     2041 tgaccggcca ctcgggcgcg ggcaagtcga ccctcgccgc cctgctcggc gagcgcggct
     2101 ggcgcttcat gggcgacgaa ttcgctctgc tcggcctcga cgacggcctg ctccgcccct
     2161 tcccgcgcgc cgtcagcctg aagaacgaat cgctcgccct gttcgacggc atcgatcccg
     2221 gccggctcgg tccggtgctc gaaggcactc ccaagggccg gatccgccat ctccgcccga
     2281 accgggaggc ggtggcgcgg atggccgagc cggcgcgccc cgtgctgatc ctgttcccgc
     2341 gcttcggccg cgacctcgat ccggccgttc gcccggtcgg ggcggcggag tcgttcatgc
     2401 gcctcaccca ggcctcgacc aattacgtcg ggctgggcga acgcggcttc gacgccctcg
     2461 cggccctggt cggcgcggtg ccgtccaagg ccgtcgacta tgccgacgcc gcccaggccg
     2521 aggcgctggt cgacgatttg tggcggggcg cgtgaaggac gcccgcctcc tcgtccgcct
     2581 gctcgccgat ccggcccgcg cccgggaggt ggccgactgg acgtcgctga tcgccgtcgc
     2641 ccgcgccgaa tcgctcgccg gcagcctcgc ctggcggctg gagggggtcg atcttccgcc
     2701 gccggtcgaa gccctgctcg aggacgcacg ccgcgacagc gaggcgggcc gggcccaggc
     2761 gctgtgggag gcggagatgg cccggcgggc gatcgcaccg ctcggcgttc cggtgatcct
     2821 gctcaagggc tccgcctatg cggcggccgg cctcgaggcg cgccgcggcc gctcgatcgg
     2881 cgacctcgac atcctcgtgc cccgccccgc cctgcccgac gtcgagcggg cgctgctcgc
     2941 cgccggctgg gaatgggtga aggaggattc ctatgacgac caatattacc ggcgctggat
     3001 gcacgagctg ccgccgctga tccaccgcga gcgcgaccgg atgatcgacg tccaccacgc
     3061 catcctgccg ctgacggcgc ggcccacgcc cgacatggac tcgatgctcg cggcgcgcgt
     3121 gccgctcgcc gaaggccttt tcgtcctgtc gccgccggat atgatcgtcc acgccgccgc
     3181 ccacctcttc gccgacggcg agctggccgg cggcctcagg aacctgtggg acatcgaccg
     3241 gctggtccgg cagttctccg ggcaaggcta tgaattcacg gtggagctgc gcgaacgcgc
     3301 ttgggagcac gacctcaccg cacccgtcgc gcgggcgctg cgcctggccc gccggctcta
     3361 tgggaccccg atcgacgacc ggctcgccgg ccgttcgccc gtttccgacg ccctgtacct
     3421 gcggcggctg ctggc
//
LOCUS       DASVQZ010000299         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_62169, whole genome shotgun sequence.
ACCESSION   DASVQZ010000299 DASVQZ010000000
VERSION     DASVQZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62169"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..648
                     /locus_tag="VF547_08165"
     CDS             178..648
                     /locus_tag="VF547_08165"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8262833.1"
                     /translation="MQGGLSWLFVALLAAALLVAAIGDWRTRIIPNWLNAVIALLAVP
                     FWWSIGLDPWPGVALQLALGAAVFALFAIAFRFGAMGGGDVKMAAALALWLPFAGVVK
                     LLVVMSIAGGVLTVAMLAAHRLAKAAGQPEIPYGIAIAFGGFWLIGERFLNQFV"
     gene            693..1751
                     /gene="cpaB"
                     /locus_tag="VF547_08170"
     CDS             693..1751
                     /gene="cpaB"
                     /locus_tag="VF547_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX8262834.1"
                     /translation="MDVKKLVLLIGALIVAAVTAVTAKNMFSGAGAPSAAAKPAVPAG
                     PEVLVATRALPVGTIIDAEALRFQRWPEGLVQPAYYIKGKPGVSPQSLIGTVVRNEIT
                     AGQPFTQGSLIKPGERGFLAAALGPGMRAVTVAVSATSGVAGFVFPGDRVDIVLTQEV
                     KGGGDGTPLKVSETIMRNVRVLATDQRMDAKGEDGKAVVQRFSMVTLEATPKIAEKIA
                     VAQTIGQLSLSLRSIADNSAELERAIASGEVKVPQTDDPKAERRMLLAIASQPIDTAS
                     TYTIGSDVSRFQRATVPGKGVKEPSDNSRIADAIGSLAAAAAGRTPGSGGGAAVPTGP
                     VVRVARGNSVTLVPVGAK"
     gene            1753..>2033
                     /locus_tag="VF547_08175"
     CDS             1753..>2033
                     /locus_tag="VF547_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEX8262835.1"
                     /translation="MSKLNIMRRATVGTAIAAVIAASLAGAVPAPAMAQPATGSYRPT
                     KQVLLSVGEGEVISLPRNVVDVWTSNPGVADVNVTSPRQLGLFGKEAGEA"
BASE COUNT          283 a          742 c          684 g          324 t
ORIGIN      
        1 gccgcgcgcc gggccgccct cgggctggcg ccgctccagc gtccgatagg tccagccgtc
       61 cgccgcctgc cagcgccgga agcgggcgtc ggcgggaatc gcgcggcggt cgaacgcgtc
      121 gctgtgcatc ggcgccggtt acggtttcga aagctgttcc gtctagtcct gctccccatg
      181 caagggggac tttcctggct tttcgtcgct ttgctcgccg cggccctgct ggttgcggcg
      241 atcggcgact ggcggacccg gatcatcccc aactggctca atgccgtcat agcgcttctc
      301 gccgttccgt tctggtggag catcggcctc gacccgtggc ccggcgtcgc gctgcagctg
      361 gcgctcggag cggcggtttt cgccctgttc gccatcgcct tccgcttcgg cgcgatgggc
      421 ggcggcgacg tcaagatggc cgccgctttg gcgctctggc tgccgttcgc cggggtggtg
      481 aagctgctcg tcgtcatgtc gatcgccggc ggcgtgctga cggtggcgat gctggccgca
      541 caccggctgg cgaaagccgc cgggcagccg gagataccgt atggcatcgc catcgctttt
      601 ggcggttttt ggctgattgg cgaacggttt cttaaccaat ttgtctgaca tgggagcctg
      661 caatttgagc gggctaggag gcgactggcg tcatggatgt taaaaagctc gtgctgctga
      721 tcggagcgct gatcgtcgcg gccgtcaccg cggtgaccgc caagaacatg ttcagcggag
      781 ccggcgcgcc ctcggccgcg gccaagcccg ccgtccccgc cggtccggaa gtcctggtcg
      841 ccacccgcgc ccttcccgtc gggaccatca tcgacgccga ggccctgcgc ttccagcgct
      901 ggcccgaagg actggtccag ccggcttact acatcaaggg caagcccggc gtcagtccgc
      961 agtcgctgat cggcaccgtg gttcgcaacg agatcaccgc cggccagccc ttcacgcagg
     1021 gttcgctgat caagccgggc gagcgcggct tcctggccgc cgccctgggg ccggggatgc
     1081 gcgcggtcac cgtcgcggtc tccgcgacca gcggcgtcgc cggcttcgtc ttcccgggcg
     1141 accgggtcga catcgtcctc acccaggagg tcaagggagg cggcgacggc actccgctca
     1201 aggtctccga gacgatcatg cgcaacgtca gggtgctcgc caccgaccag cgcatggacg
     1261 ccaagggcga ggacggcaag gcggtggtcc agcgcttctc gatggtcacc ctcgaggcga
     1321 ccccgaagat cgcggagaag atcgcggtcg cccagaccat cggccagctg tcgctgtcgc
     1381 tccgctcgat cgccgacaat tccgccgagc tcgagcgcgc catcgcctcc ggcgaggtga
     1441 aggtgccgca gaccgacgac cccaaggcgg agcggcggat gcttctcgcc atcgccagcc
     1501 agccgatcga caccgcgtcg acctacacga tcggctcgga cgtctcccgc ttccagcggg
     1561 ccaccgtccc cggcaagggg gtcaaggagc cttcggacaa cagccgcatc gccgacgcca
     1621 tcggctcgct cgccgccgcg gccgcgggcc gcacgccggg ctccggcggc ggcgcagccg
     1681 tccccacggg ccccgtcgtc cgggtcgccc gcggcaactc tgtcaccctc gttccggtcg
     1741 gagccaagta agatgagcaa gctcaacatc atgcgccgag cgactgtcgg caccgcgatc
     1801 gccgcggtga tcgccgccag cctggcgggc gccgtccccg ccccggcgat ggcccagcct
     1861 gccaccggca gctaccgccc gaccaagcag gtgctcttgt cggtcggcga aggcgaggtc
     1921 atctccctgc cccgcaacgt ggtcgacgtc tggacctcga accccggcgt ggccgacgtc
     1981 aacgtcacca gcccccgcca gctcggcctg ttcggcaagg aagccggcga ggc
//
LOCUS       DASVQZ010000300         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_259007, whole genome shotgun sequence.
ACCESSION   DASVQZ010000300 DASVQZ010000000
VERSION     DASVQZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259007"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VF547_08180"
     CDS             <1..450
                     /locus_tag="VF547_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8262836.1"
                     /translation="PSLAIKHGAPAMKERGRGSIVCTASVAGLRAGAGGPAYSASKAG
                     VINLVRVAATQLAGSGIRVNAICPGLIETGMTRPVYEYARASGKEDRIGHLNPLKRGG
                     VPDEIANAALFLASDEASYVNGHALVVDGGLSVSHPFSHQEYGRTSV"
     gene            511..957
                     /locus_tag="VF547_08185"
     CDS             511..957
                     /locus_tag="VF547_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8262837.1"
                     /translation="MLRKWLLPILAVTAAAPAAACDTSPLKIFYAPGSLRLSEQDLEV
                     LDYMRLVAGETNFIRLTGHTDTAGPAMANMTLSQRRVDAARAYLIKLGMPPSRILTQS
                     FGESRSITALDDGRADRRHRFVLIELLSPEEAKKGRAGRPKMTCGG"
     gene            complement(974..1735)
                     /locus_tag="VF547_08190"
     CDS             complement(974..1735)
                     /locus_tag="VF547_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8262838.1"
                     /translation="MSFRELVIAYLQYPAILAYLALAAAAVGLFAARPAPLAPTLIAV
                     AAATFVYPLVWYLLHRFVLHSKWMWKSRWLAPTWKRIHYDHHQDPNHLEVLFGALHTT
                     LPTIAIATLPLGWAIGAPFASPAHPDAALGAALAAFATGLVTTCFYEFVHCIQHLAYK
                     PRRKWLAEMKRRHMAHHFHDETGNFGITSFLWDKLFGTFYDKASDRPTKSPTVFNLGY
                     TPEVAEVYPHVARLSGGVATDHPRERVRQEASAAS"
     gene            complement(1888..3429)
                     /locus_tag="VF547_08195"
     CDS             complement(1888..3429)
                     /locus_tag="VF547_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX8262839.1"
                     /translation="MAASPPPDPDENVTRGEVARGAGLVGLSRSAALIEVVSQPLFIW
                     LYGLPTYGIYVALWAAINFVENVVDLSLTSALQRIVPTGDEDEAHGAVKAAFLVTVLP
                     AAAIALLVTLNAGWVAGFFSAAPADKAHLPTAVALFAWGLPLWTFIEIATSAARARRA
                     FGPEIRLRLFWEQIARIAFAVGLFGLGAGSLGLVLAHLGSLSLTALLCLPLLARFYDP
                     RRLVRARIDGRAFRLLLTTGLALLPSNASRRFLIDGPAVIINVLLPGARGAIGAGLFE
                     IARKLATLPLAVRQAFQYVMAPVAAHQARADRAAIAPLYHFASRLSTALVVPLAGWLA
                     FAGPDILSIYRPEVMAALPLLYILVAARAAEAIVGPATPIVEMTGHRILPLVNSLVGV
                     ATWAALCVLLVPRWGAPGMAVAVGVATVAIAYCATLELQLTEGLTPFDRKLFLGLAVA
                     LAGVGLMALAAWATRGPARFASVTLLWAASSWCAVRLGLVRGDREALGALSRSLRLVP
                     RAPRP"
     gene            complement(3528..>4226)
                     /locus_tag="VF547_08200"
     CDS             complement(3528..>4226)
                     /locus_tag="VF547_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262840.1"
                     /translation="LLTAMFGVVADGASRWVSVGPISVQVSLIVLPAMIVLYARRPDP
                     VGTAGMAVAALALAIQPDRAMAGVLLAGLLALLATRPGRLPALACAAAALGFGWTLLA
                     PDRLPSSAFVDGVLFSAFDVHPVAGLAVVLGAASLLVPAAIGLARGAGDRAALLAFGG
                     CWSAVIAAAALGNYPTPLVGYGGSAVLGYLLSAALLSGAAPGSRREAAPASQTAAVGS
                     PARPLSELRAAPLA"
BASE COUNT          701 a         1412 c         1601 g          512 t
ORIGIN      
        1 ccgtcgctgg cgatcaagca cggcgcgccg gcgatgaagg agcgcggccg cggctcgatc
       61 gtctgcaccg ccagcgtcgc cggccttcgc gccggcgccg gcggcccggc ctattcggcg
      121 tccaaggcgg gcgttatcaa cctcgtccgc gtcgccgcca cccagctcgc cggctcggga
      181 attagggtga acgccatctg tcccggcctg atcgagaccg gcatgacccg cccggtctac
      241 gaatatgcgc gggcctcggg caaggaggac cggatcggcc atttgaaccc gctcaagcgc
      301 ggcggagtgc ccgacgagat cgccaacgcc gccttgttcc tcgcctcgga cgaagccagc
      361 tacgtcaacg gccacgcctt ggtcgtcgac ggcggcctgt ccgtctccca tccgttcagc
      421 caccaggaat atggccgaac ctcggtctga cccttgaatt ccctcgacac gcccccgcgg
      481 cccggcgaaa ttgcccatgg aggcgagtct atgcttcgaa aatggctctt gccgatcctg
      541 gcggtgacgg cggcggcacc tgccgccgcg tgcgacacct ctccgctgaa aatcttctat
      601 gcgcccggct cgctgcgcct ttccgagcag gacctcgagg tgctcgacta tatgaggctc
      661 gttgccgggg agaccaactt catccgtctc accggccata ccgacacggc cggtcctgcc
      721 atggcaaata tgaccttgtc ccagcgacgg gtggacgcgg cgcgcgccta cctgatcaag
      781 ctgggcatgc cgccgtcacg gatcctgacc cagagcttcg gggagagccg gtcgatcacg
      841 gcactcgatg acggccgcgc cgatcgccga caccgcttcg tgctgatcga gctgctgtcc
      901 ccggaagagg cgaagaaggg ccgagccggg cgaccgaaga tgacctgcgg cggctgacgc
      961 taagcctcgc gggtcagctg gccgcgctgg cctcctgccg cacccgctcc ctcggatgat
     1021 cggtggcgac tccgcccgac agccgcgcca cgtgcggata gacctcggcc acctccggcg
     1081 tgtagccgag gttgaacacc gtcgggctct tcgtcggcct gtcgctcgcc ttgtcgtaga
     1141 aggtgccgaa cagcttgtcc cacaggaagc tggtgatccc gaaattgccg gtctcgtcgt
     1201 ggaaatggtg ggccatgtgg cggcgcttca tctcggccag ccatttgcgc ctgggcttgt
     1261 aggcgaggtg ctggatgcag tggacgaact cgtagaagca ggtggtgacg aggccggtgg
     1321 cgaaggcggc cagagccgcg cccaaagcgg cgtcgggatg ggccgggctc gcgaacggcg
     1381 cgccgatcgc ccagccgagc ggcagggtgg cgatcgcgat ggtcggcaag gtggtgtgca
     1441 gggcgccgaa cagcacctcg agatgattgg ggtcctggtg gtggtcgtaa tggatccgct
     1501 tccaggtcgg ggcgagccag cggctcttcc acatccactt gctgtggagg acgaagcggt
     1561 ggaggaggta ccagacgagg ggatagacga aggtcgcggc ggcgaccgcg atcagggtcg
     1621 gcgcgagcgg cgccgggcgg gccgcgaaca ggcccacggc ggccgcggcc agcgccagat
     1681 aggcgaggat cgcgggatat tgcagatagg cgatgacgag ctcgcgaaag ctcatccggt
     1741 ccagatgatg ctcccgcctc cagaagctgg ggcggatcgt cagagcgggt ttcacagtcc
     1801 taccaatcct cgtttgcgcg gtcgcggcgg gggggccccg cggcccgcca atatggggct
     1861 ttccactggc ggcgcaatgc ggcgctttta gggcctcggc gcgcggggga cgagcctcag
     1921 gctgcgcgac aaggcgccga gcgcctcgcg gtcgccgcgg accaggccga gccggaccgc
     1981 gcaccagctc gaagccgccc acagcagggt gaccgaggcg aagcgggccg ggccgcgggt
     2041 cgcccaggcg gcgagcgcca tcagcccgac tccggccaga gcgacggcca ggccgaggaa
     2101 gagcttgcgg tcgaacggcg tcaggccctc ggtgagctgg agctcgagcg tcgcgcaata
     2161 ggcgatggcg acggtggcga ccccgaccgc gacggccatt ccgggcgccc cccagcgcgg
     2221 cacgaggagg acgcagaggg ccgcccaggt ggcgactccg acgaggctgt tgacgagcgg
     2281 gaggatgcgg tggccggtca tctcgacgat cggcgtcgcc gggccgacga tcgcttcggc
     2341 cgcgcgcgcc gcgacgagga tgtagagcag gggcagtgcc gccatcacct ccgggcggta
     2401 gatggagagg atgtccggcc cggcgaaggc cagccagccg gcgagcggga cgacgagcgc
     2461 ggtcgagagg cggctggcga aatggtagag cggggcgatc gcggcccggt ccgcccgggc
     2521 ctggtgggcg gcgaccggcg ccatgacgta ctggaacgcc tggcgcacgg cgagcggcag
     2581 cgtggcgagc ttgcgggcga tctcgaacag gcccgcgccg atggcgccgc gggcgccggg
     2641 aagcaggacg ttgatgatca cggcgggccc gtcgatcagg aagcggcgcg aggcgttgga
     2701 gggcagcagg gcgaggccgg tcgtcagaag caggcggaag gcccgaccgt cgatccgcgc
     2761 ccggaccaag cgccgtggat cgtagaatcg ggccagcaag ggcaggcaca gcagggcggt
     2821 cagggagagc gagccgagat gggccaggac gaggccgagg ctgcccgcgc cgaggccgaa
     2881 caggccgacc gcgaaggcga tccgggcgat ctgctcccag aacaggcgca gccttatctc
     2941 cgggccgaag gcgcggcggg cgcgcgccgc cgaggtcgcg atctcgatga aggtccacaa
     3001 gggcaggccc caggcgaaca aggcgacggc cgtcggcagg tgcgccttgt cggccggcgc
     3061 ggccgagaag aagccggcga cccagccggc gttgagggtg acgagcaggg cgatcgcggc
     3121 ggcgggcagg acggtgacga ggaaggcggc cttgacggcg ccatgcgcct cgtcctcgtc
     3181 cccggtggga acgatccgct ggagcgcgct ggtcagcgac aggtcgacga cgttctcgac
     3241 gaagttgatg gccgcccaaa gcgcgacata gatgccgtag gtgggaagcc cgtagagcca
     3301 gatgaacaaa ggctgcgaga ccacctcgat cagcgccgcc gaccgcgaca agccgacgag
     3361 gccggcgccg cgcgcgacct cgccccgggt gacgttctcg tccgggtcgg ggggcgggga
     3421 agcggccatc ggcgcttgct ttggcgcggg gacggggtgg ggggcaagtt ttactcggcc
     3481 ggcggcggcc gcgagcggac ccgatggcgg ccggacgagc agccggctca ggccagcggc
     3541 gccgctcgaa gctccgacag cggccgggcg gggctcccga ccgcggccgt ctgcgatgcc
     3601 ggcgcggcct cacgccggga cccgggcgcg gcgccggaca gcagcgcggc gctgagcagg
     3661 tagccaagca cggcgctgcc gccatagccg accagcggcg tgggataatt gcccagcgcg
     3721 gccgcggcga tcaccgccga ccagcacccg ccgaaggcca gcagcgccgc ccgatcgccc
     3781 gctcctcgcg ccagtccgat cgcggcaggg acgaggagcg aggcggcacc gaggacgacg
     3841 gcgaggccgg cgaccgggtg gacgtcgaag gccgagaaga ggacgccatc gacaaaggcg
     3901 gacgacggga gccggtccgg ggcgagcagg gtccagccga agcccagggc cgccgcggca
     3961 caggccaggg cgggcaggcg gccgggtctc gtggccagca aagcgaggag gccggcgaga
     4021 agcactccgg ccatcgcccg atcgggctgg atcgccagcg ccagggcggc caccgccatc
     4081 cccgccgtcc cgaccggatc gggccgcctg gcgtaaagga cgatcatcgc cggcaggacg
     4141 atgaggctga cctggacgct gatcggcccg acgctgaccc atcgcgacgc cccgtcggcc
     4201 acgactccga acatggcggt cagaag
//
LOCUS       DASVQZ010000301        24868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_449138, whole genome shotgun sequence.
ACCESSION   DASVQZ010000301 DASVQZ010000000
VERSION     DASVQZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 24868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24868
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449138"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF547_08205"
     CDS             complement(<1..416)
                     /locus_tag="VF547_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262841.1"
                     /translation="MRGILRLAPLLLAAEPAAERSAERELMVLTVANMAATSGLAEAR
                     RLDPAVLSAMREVPRHRLVPEAVRAQAYQNRPLPIGYEQTISQPFMVALMTHLLGVRS
                     ADAVLEVGTGSGYQAAILSRVARQVYTIEIVEPLAER"
     gene            complement(413..1762)
                     /locus_tag="VF547_08210"
     CDS             complement(413..1762)
                     /locus_tag="VF547_08210"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX8262842.1"
                     /translation="MTMPPNLLLAAALALPAMLAGSEGRPTASARPIPAALVQSLRPV
                     AGADSDYSDLFAAAAGARRILLGESTHGTHEYYRERARITERLIRTHGVTAVAIEGDW
                     SATQRVNLYVRGLGSDRSAEQALRGFTNFPQWMWANGDFREFVERLRALNLERPPEQR
                     VGLYGMDVYDLFDAAEAVQAYLKRTAPEAARAAARHYRCFARFGRDRWAYGVATRDPR
                     RACRTQAEAVRAELRRLPVPSDPAERESRFAALRSAASVAAAEEYFRTASAGSLAWNV
                     RDRHMADNVDEIADHVEAMSGRPAKVVMWAHNTHSGDARATFAADRGELNLGQLMRQR
                     HGDAAFLIGFLSHSGTVFAAPEWDAPGRRHDMRPALPGSYSDLFHRAGLPAFSLLIRG
                     NAAVSRHLAGPMLERAIGVVYLPSSERTAHYFEARLAEQFDAVVYFDRSKAVTPVRR"
     gene            complement(1826..2002)
                     /locus_tag="VF547_08215"
     CDS             complement(1826..2002)
                     /locus_tag="VF547_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262843.1"
                     /translation="MSHDEREYYERRAEAEIELAQAAAHRRVVQAHYELAAAYLDRIH
                     GDEPRSEGGLFAQP"
     gene            complement(2158..3657)
                     /locus_tag="VF547_08220"
     CDS             complement(2158..3657)
                     /locus_tag="VF547_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262844.1"
                     /translation="MRALILAASMLLCAAAAASAQVRGAPPPVTAPAPELLKLAAEVH
                     RTGPKLARLWPGFWPPDQAFIIYRPGQGALLVAADAPPPPFAALPAAGVPKALRGRAW
                     FRPGNLDGVGRPFILGYPIGAGRTAVLVLADEAAKDLATLIFHEQFHAYQRKAFRHPS
                     GSQFVAPTAVPDRVSFAAGAETERRILASALTMRRGRMRDGLLHQYLALRREREKSLP
                     PGVVRVEGQLEKVEGTAKFIDRAAVALGPEPDGNLAGLIAEVLREDLGAGGQPFLTAW
                     FRSRSYGVGAALTYLLRDLDPKGWRSAVESADSLDERLAVLTGFGSVRDPGGLAEGAR
                     SAFGYAATKAALEPKLRAAERKEIKSAEEFYALGAHRIVFEVTAPAQSGDRGTEMGFA
                     SGPGGMTLLGEAQLVLPDPRLVQITLPFGALSVKDRPFMSEGGRTSRYTILVPAAPSL
                     NGRAGLGAGEHRFERLDLTAEGVRIGVERPVTVVVGQHDTIIRYAVAAD"
     gene            3878..4435
                     /locus_tag="VF547_08225"
     CDS             3878..4435
                     /locus_tag="VF547_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262845.1"
                     /translation="MADLGFSGVVEKFEEHWGKRPTRWLLLIIGLGVAAVCVGAIWEW
                     LISPLLAFIGSPQRSQTIWALVLAIVGIGAGISLTTSLTTALARWRRIRTLNQIVSET
                     ETKADQILDRATGLVSKASQDNLQTRALLEASLLVTKALTEKDPTIGEDERAERLGEI
                     ERGLQELQELRETQEMRKKRGEDGE"
     gene            complement(4986..5618)
                     /locus_tag="VF547_08230"
     CDS             complement(4986..5618)
                     /locus_tag="VF547_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX8262846.1"
                     /translation="MSWTDERIDRLKELWSQGMTASQIADELGGVSRNAVIGKAHRLG
                     LQSRPSPVKPNEGGSEAGPSPFEPEAASEAEPDEDRADAEPDARQEPAAAPAAPQAQI
                     RSIGPGGFVRQAPGEQQSPIPPAPPRRLVPAKPSPEIADKTGLLDLNEKICKWPIGHP
                     GEPDFHFCGAPANPGFPYCVEHCGVAYQAQIPRRDRRPPPPLPFGGPRIR"
     gene            5699..6514
                     /locus_tag="VF547_08235"
     CDS             5699..6514
                     /locus_tag="VF547_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8262847.1"
                     /translation="MRADPGVPVIRNVNWGGLGTLYVKEVRRFFKVQLQTVWAPAVTT
                     LLFLVIFTVALGRSGRTVELDGASFRFADFLAPGLIVMGMLQNAFANSSFSLLVGKIQ
                     GNIVDYLMPPLSTAELIAALVGAAVTRAVLVGFAVFLAMQLWPGVDATPVELCAVAWF
                     GLMGSMMLAFLGLMTSIWAEKFDHAAAVTNFVVAPLSLLSGTFYSIDSLAPAFRTISH
                     ANPFFYVISGFRYGFLGAADSPILAGALLLLAINLVLGGLCYAMLRAGWKLKN"
     gene            6535..6876
                     /locus_tag="VF547_08240"
     CDS             6535..6876
                     /locus_tag="VF547_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262848.1"
                     /translation="MSTTLILAAFLSAAPAAAPADARSPYRVDRSLSDADRLSELRID
                     VTSAYDGGRIDRATATEFYLAIERIRRQMIRMGIQVGYRQRVRLRARIDGLYARWAKR
                     QSLNAEVRSGK"
     gene            6992..7369
                     /locus_tag="VF547_08245"
     CDS             6992..7369
                     /locus_tag="VF547_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262849.1"
                     /translation="MRKILMSALMLSTVVAVAPAAAQYGHRDRGHHEWNRGGPSRQAI
                     NELLRDLRRAENGIERSVQRRVISPREAIGLRREANRIERRLSLAGRDGISHREFASL
                     RMQVNRLQQRLRMERRDRDGRRY"
     gene            7484..8671
                     /locus_tag="VF547_08250"
     CDS             7484..8671
                     /locus_tag="VF547_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8262850.1"
                     /translation="MAITPLMPVYPRSPVRPVRGEGVWLFGEGGEKYLDFASGIAVNL
                     LGHGHPHLTKAIQEQAATLIHVSNLYGSPQGEAFAQRLIDNSFADTVFFTNSGAEAVE
                     CSIKTARRYHHHHGRPEKHVLITFRNAFHGRTMATISATDQPKLRDGFAPMLPGFTVV
                     DFDDLDAAEAAVDDSTAGFLVEPVQGEGGIRPASAEFMRGLRRICDERDLMLVLDEVQ
                     CGVGRTGKLYAYEHYGIEPDILASAKGIGGGFPMGACLATEKAAAGMLVGTHPSTYGG
                     GPLAMAAGQAVLDIVARPEFLERVTRMGERLRGALEQMIPNHDHLFESVRGLGLMLGV
                     KMKTDSRAFVGHLREHGLLTVAAGDNVMRVLPPLIIEEEHVTEFVERLSEAARHYEVP
                     QAA"
     gene            8747..9664
                     /locus_tag="VF547_08255"
     CDS             8747..9664
                     /locus_tag="VF547_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX8262851.1"
                     /translation="MTSPDFQAEARAANLDEALGFTIPARNARGRLVRLGPLLGDILS
                     AHDYPAPIARILSEALVLSALLGSLLKEAEGQLTIQAQTESGIVELLVVDYRAGELRG
                     YVRFDPDRLGDLPTHRDLAALFGKGYLAITFDHPKAEERYQGIVPLEGASLGEAAQNY
                     FSQSEQLPSVVRLAVDDTGHIAGGILVQHLPEGEEGRDRLHTRLDDPEWEHVRLLAET
                     IKGHELTDPSLPLGEALWRLFHEEEVRVLAAVPLRKGCRCNLDYVRGVIARFGAAERA
                     EMVDEDGLISVDCEFCSRIFPIPLSDFED"
     gene            9761..10135
                     /locus_tag="VF547_08260"
     CDS             9761..10135
                     /locus_tag="VF547_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262852.1"
                     /translation="MGLVGGLAVAAPVLALSLPSLEALAKLERGRWRISESQSGAARS
                     LCLADPAEFLQLEHRGVACSREVLASEAGSVTVQYTCPGRGFGHTSVRVETPRLARID
                     TQGLADGRPFSYRAEARKVGSC"
     gene            complement(10163..10708)
                     /locus_tag="VF547_08265"
     CDS             complement(10163..10708)
                     /locus_tag="VF547_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262853.1"
                     /translation="MSNSTITPRFASRLTARLALGLALICGLTPASAWDERSTPEDLR
                     RFVSVTRNLEQAPLEPALKADREWALTWLTNAPDVTVTICADALGSLVKSNYRYAGEI
                     ILQNSFAMAALAIEHPEKANDPNTQQLAGLEGALNAYRSILRDKPEARSSALDGLLKT
                     QSRGELPAFVQKAWIRCSAKN"
     gene            10970..11659
                     /gene="queC"
                     /locus_tag="VF547_08270"
     CDS             10970..11659
                     /gene="queC"
                     /locus_tag="VF547_08270"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX8262854.1"
                     /translation="MSTPVRKAVVLVSGGLDSMVAAALARESGFALLALTVDYGQRHR
                     IELEAAARIAAALGAERHVLLPLDLRPFGGSALTADIDVPKGGVGPDIPVTYVPARNT
                     IFLSLALGWAEAAGSADIYIGVNALDYSGYPDCRPDFIAAFERLASLATKAGDEGGSF
                     RIAAPLLRMTKADIVREADRLGLDPGLSWSCYDPAPGGRHCGLCDSCRLRARGFAEAG
                     LADPTRYAARP"
     gene            complement(11663..12325)
                     /locus_tag="VF547_08275"
     CDS             complement(11663..12325)
                     /locus_tag="VF547_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262855.1"
                     /translation="MTFRTKYGSMAAAAAALAAAPAPAQLYISQPNLRPSAIEPSDPL
                     VGLPLPGATPAEHRANLLWNLRAGLNVAALQCQFSDYLRAVPNYNGLLAHHNVELAAA
                     YTTLNNYFKRVQGAKGQKAFDDYSTITYNNWSTLQAQMIFCQTATNIAKSALATPKGQ
                     LLPLAQARMRELRNALVPAYDPRPAYLPHLIRFRDMSLAPPPECFNKKGVLRRACGGT
                     AK"
     gene            complement(12531..12824)
                     /locus_tag="VF547_08280"
     CDS             complement(12531..12824)
                     /locus_tag="VF547_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262856.1"
                     /translation="MRDLLSKVMTGSMVAGAALLVAACGGGEDAAANNTATNYVEDPL
                     MGGNDVTPVDALNGTDANLGVTTDAANVSTTTTTNTTTTDTTTNTGTGNTTGM"
     gene            complement(12920..13576)
                     /gene="lipB"
                     /locus_tag="VF547_08285"
     CDS             complement(12920..13576)
                     /gene="lipB"
                     /locus_tag="VF547_08285"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX8262857.1"
                     /translation="MADDDEIEWRVTPGLSPYPETLAAMEARAAAIRSGQASELVWLL
                     EHPPLYTAGTSADPAELFNPQGFPVYEAGRGGRYTYHGPGQRVGYVLLDLERRGRDVR
                     HYVWCLEGWVIATLARLGIEARRAEGRIGIWADGPGGEAKVGAIGVRVRRWVTYHGFS
                     VNLSPQLSHFSGIVPCGIADFPVTSLDEMGASAGQDRFDLALREGLCDFLRSLEGGRE
                     "
     gene            13950..14666
                     /locus_tag="VF547_08290"
     CDS             13950..14666
                     /locus_tag="VF547_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262858.1"
                     /translation="MDEKLLNLPWEVQLALASGYAAYMLAYVGIREHHKAIDVTFRTL
                     AFGLCATAVLALSPSQLGWPRAVAAVLVALMGGVLWRFWLAEGMQRAIRNVNLSWSDD
                     TPSAWSRVTQHNSRHFVTQVTVHLDDDSWLFCDATGSFGAAPFGPCIFGTNGDIALYV
                     THSAAPGEDFKAVGNAVRDTHHGDELTWVPASRIRKVSVRLKGSVNASSAEAGQAQAQ
                     AQAVEAVPSASPSAVPNPAE"
     gene            14903..15613
                     /locus_tag="VF547_08295"
     CDS             14903..15613
                     /locus_tag="VF547_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262859.1"
                     /translation="MTMDIAWFLNERLAFLRVHFDICVSAFEERKRKIEAGEPPFNNP
                     PIDEDGEPPFLQEWIDAQTSIELIGVACLSLLSDAFKLYLNLLQHDQLHFVFNDDEKR
                     RLKSEGFVPAYRDALSEIFDTDWQASGIDFAVIEQVVLARNRGQHGTALTSFMLTHDD
                     RTIAKHPRPLFLRADEARALDTAAVRMGGFLNPLLVVTRETLFAAIEEVEKLARWIDA
                     NMDRALRWQIEQRSLKSS"
     gene            complement(15705..16172)
                     /locus_tag="VF547_08300"
     CDS             complement(15705..16172)
                     /locus_tag="VF547_08300"
                     /inference="COORDINATES: protein motif:HMM:NF025725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HEX8262860.1"
                     /translation="MYVFDNSPISVLFRNYYRRRFPTLWDRFDALVADGRIVSTREVY
                     REAVDSSFENLRDWVTAHQSVFHVPTAAEGSFVGRIYAVRHFQQNIEQKKLLKGGRNA
                     DPFVIAKAATEGRCVVTMEQFKENGAKIPNICAHFGVDCLSLERFMEREGWEF"
     gene            complement(16172..17320)
                     /locus_tag="VF547_08305"
     CDS             complement(16172..17320)
                     /locus_tag="VF547_08305"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX8262861.1"
                     /translation="MTEQIPINPAVLIWARERAGLSRDEASERFKRFAEWEQGEAAPT
                     YPQLEQLADAFKVPIAVFFFPEPPNVPPINETFRTLPEAELDRLPSRIRLLMRKAKAM
                     QLSLAELTQGRNPAERLITRDLQFGPNVNLVEMTKAVREYIGVSLEQQTAWKDDEAAL
                     KEWRTALQRVGIFVFKDAFRADELSGFCLYDVEFPIIYVNNSSAKTRQIFTYFHELAH
                     LIFHTSGIDKVRDRYIQRLAGDSKQIEILCNGFAAQFLLPEEAFARAVAGHPATEATA
                     ELVASQFHVSREVVFRRFLDQGRIGESQYLEAVERWKAQRQAGAGEGGVGGNHYWTKL
                     AYLGREYVALALSQFHQNRIDENQLADYLDTKPKNVGTLEDYFARGNV"
     gene            18087..18842
                     /locus_tag="VF547_08310"
     CDS             18087..18842
                     /locus_tag="VF547_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262862.1"
                     /translation="MKFSYSAVWADVMALLRTHASLIATVAGVFIFLPGLLVAYFLPQ
                     PQTQELARIGEVWVEYARLNWPWMLLNSLFNIVGSIAILRLIFERGVTVGAAIAGALA
                     LFVPYFLTSLLSSVLIGLGFILLILPGAYLLGRLSPVTAIVVAERRRNPIDALKRCFE
                     LTKGHGWAVLGLLLLVAIPASIAIRVATMLLGILFVLVAGQDLGDLLVLIVRTAGAAA
                     LAALMLVLNAAIYRQLSQSRPEAAAAAAADPGP"
     gene            complement(18857..19438)
                     /locus_tag="VF547_08315"
     CDS             complement(18857..19438)
                     /locus_tag="VF547_08315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8262863.1"
                     /translation="MLTPLPEGHVGAVVTYLEMTRRPPPGPLPASPLRLEPWPASDPA
                     RYRALFRKVGERWLWYSRLVMDDARLRREMAEIHAVLDPAGIEVGLIELDLRTPGDCL
                     IRFLGLVPELAGRGHGRWLFAQTLALAWRPGVERVRVNTCTLDHPAALPAYLKAGFMA
                     YERAFESFPDPRLNGLLPRDAAPHLPSIGGGAG"
     gene            complement(19520..19852)
                     /locus_tag="VF547_08320"
     CDS             complement(19520..19852)
                     /locus_tag="VF547_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262864.1"
                     /translation="MTGRAGRDWRAIGCAAALAASIAFPLGLLIGGGGAPSVEPARPA
                     SRARPPATRTPAHRDVYSPRVASDPYVIEQQRRVLRALEASCRQSGRHCAEAEQAGRR
                     IEEAEARR"
     gene            complement(19849..19980)
                     /locus_tag="VF547_08325"
     CDS             complement(19849..19980)
                     /locus_tag="VF547_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262865.1"
                     /translation="MTVVPLSAIRAPAALCLLLALAILAFGAAFHLYLLLLAQALPR"
     gene            complement(20033..20686)
                     /locus_tag="VF547_08330"
     CDS             complement(20033..20686)
                     /locus_tag="VF547_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8262866.1"
                     /translation="MTITITAFERSPDQGRGLARDMPVRWALEEVGQPYDVRLVSFEA
                     MKRPEHRRLHPFGQIPTYEEGGLVLFESGAIVLHVGERHRGLLPDDADARARAIAWMF
                     AALNSMEPPIVDLEAAEHLEGDRPWHAERLPLLRDRARRRLDELSERLGGSDWLDGGF
                     SAGDLLMVTVLRRLEGSGLLDGYPGLCAYIARGEARPAYRRAFDAQLAVFTAGSASA"
     gene            20827..23502
                     /locus_tag="VF547_08335"
     CDS             20827..23502
                     /locus_tag="VF547_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8262867.1"
                     /translation="MDKPLVDGPVGASVPADAAGRASVRENEALFRFLDALGRATAES
                     ADADEVLSITARMTAEHLGLSNCAYADMDEDGDGFTIRGDWHAPGSPSIVGRYRLADF
                     GKLAVQELKAGRPLIINDNLREIAPEEAKAFQDIGIAATICMPLVKSGRLVALMAIHH
                     RVPHQWTVYELTAVREVTERSWTHVERVRSDAELRASNARFRAAIRATEGVVWTNDAQ
                     GRMIGDQPGWSALTGQSFDEYQGFGWSEAVHPDDREPSVAAWNAAVAARRAFVFEHRV
                     RRADGRWGLFSIRAVPVLGDQGEVTEWVGVHTDITSQRAGEEALREGSQTLEILNRTG
                     AAIAAELDLERLVQMVTDAGVELTGARFGAFFYNVLDDEGGSLMLYTLSGAERSAFER
                     FPHPRATPIFSPTFKGERVVRSADITADPSYGLNPPYKGMPEGHLPVRSYLAVPVTSR
                     TGEAIGGLFFGHEDRDVFSDRSERIMVGLAAQASIAIDNARLFQAVQRANQTLEERVQ
                     ERTAELEQAHEALRQAQKMEAIGQLTGGIAHDFNNLLTVIRGSADLLRRSGLSEEKRR
                     RYVAAISDTADRAAKLTGQLLAFARRQALKPEVFDAAIRVHGISDMLRTVLGSRVRLR
                     IEDDCPDCFVEADVGQFETALVNMAVNARDAMDGEGDLTISVGPIEDSKGRGMVAVRV
                     SDTGHGIPRDRLEHIFEPFFTTKEVGKGTGLGLSQVYGFTKQSDGEITVDSEVGAGTT
                     FTLCLPKAEVGHAPRLAGRGAIDPAPGGGLVLVVEDNADVGLFASQMLGELGFATSLA
                     ANAREAIGLLEARAEEFDIVFSDVVMPGLSGIEFARLVRERWSHLPVVLTSGYSHVLV
                     EESCHEFPLLHKPYSVEALSRILAEARLSAPARGR"
     gene            complement(23560..24405)
                     /gene="hemF"
                     /locus_tag="VF547_08340"
     CDS             complement(23560..24405)
                     /gene="hemF"
                     /locus_tag="VF547_08340"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX8262868.1"
                     /translation="MTIELDEEQSRARDWFESLRDRICAEFEAIEREAGGEAAFDYTP
                     WERRDPDGAPGGGGVRGLMKGKVFEKVGVNVSTVAGRFTDEFAATIHGAGEDPRFFAT
                     GISLVAHMANPHVPAVHMNTRFLATTKRWFGGGADLNPPIPYEEDTADFHARLRAACA
                     AHDQTFYDRFRKWADEYFWIPHRGVHRGAGGIFYDHLEGPFEASFAFTRDVGEALLDV
                     FPKLVRRRMNTPFTEADKARQLEWRGRYAEFNLLYDRGTLFGLRTGGNVDAILMSLPP
                     LATWR"
     gene            complement(24527..>24868)
                     /locus_tag="VF547_08345"
     CDS             complement(24527..>24868)
                     /locus_tag="VF547_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX8262869.1"
                     /translation="PFSDRALKRAGMDYAQMAAVTRHSGWDAFERALPARLVLFTTAG
                     ALPLPEARFQPADTLLFGSESRGAPEAVHRRADLRVRIPQTSGTRSLNLAVAAGIGLA
                     EAIRQTGLWAV"
BASE COUNT         3900 a         8408 c         8480 g         4080 t
ORIGIN      
        1 cgctccgcca gcggctcgac gatctcgatc gtatagacct gccgcgccac gcgggagagg
       61 atcgccgcct ggtagccgga gccggtcccg acctcgagca ccgcgtcggc cgatcgcacg
      121 cccagcaagt gggtcatcag agcgaccata aagggctgcg agatggtctg ctcgtagccg
      181 atcgggagcg gccggttctg ataggcctgc gctcggaccg cctccggcac cagccggtgg
      241 cgcggcacct cgcgcatcgc gctgaggacc gccgggtcga gccgccgcgc ctcggccagg
      301 cccgacgtcg ccgccatgtt ggcgacggtc aggaccatga gctcgcgctc cgccgagcgc
      361 tccgccgcgg gctcggccgc gagcagcagg ggcgccaggc gcaggatccc tctcaccgcc
      421 gcaccggagt caccgccttc gagcggtcga aatagacgac ggcgtcgaat tgctcggcga
      481 gccgggcctc gaaataatgg gcggtccgct cgctcgaggg caggtagacc actccgatcg
      541 cgcgttcgag catcggtccg gcgaggtggc gcgacaccgc cgcattgccg cggatgagca
      601 gcgagaaggc gggcaggccg gcgcggtgga acaggtcgga atagctgccg ggcagggccg
      661 ggcgcatgtc gtggcggcgg cccggcgcgt cccactcggg cgccgcgaag acggtccccg
      721 aatgactgag gaagccgatc aggaaggcgg cgtcgccatg ccgctggcgc atcagctggc
      781 ccaggttcag ctcgccgcga tcggcggcga aggtggctcg ggcatcgccc gaatgggtgt
      841 tgtgggccca catcacgacc ttggccgggc gcccgctcat cgcttcgaca tggtcggcga
      901 tctcgtcgac attgtcggcc atgtggcggt cgcgcacgtt ccaggccagc gagccggccg
      961 aggcggtgcg gaaatattcc tccgccgccg ccaccgaggc ggcgctgcgc agggcggcga
     1021 agcgcgattc cctctcggcc gggtcgctcg gcaccggcag cctgcgcagc tcggccctca
     1081 ccgcttccgc ctgggtccgg cacgccctgc gcggatcgcg cgtggcgacg ccataggccc
     1141 agcggtcccg tccgaagcgc gcgaagcagc ggtaatgccg ggcggcggcc cgggcggcct
     1201 cgggcgccgt ccgcttcaga taggcctgga ccgcctccgc cgcgtcgaag aggtcgtaga
     1261 cgtccatccc gtagaggccg accctctgct cgggcggacg ctcgaggttg agcgcccgga
     1321 gccgctcgac gaactcgcgg aaatcgccgt tggcccacat ccactggggg aagttggtga
     1381 agccgcgcag cgcctgctcg gcgcttcggt cggagccgag gccccggacg tagaggttga
     1441 cccgctgggt cgccgaccag tcgccctcga tcgccaccgc ggtgacgcca tgcgtccgga
     1501 tcagccgctc ggtgatccgg gcgcgctcgc gataatattc atgggtgccg tgggtcgatt
     1561 cgccgaggag gatgcggcgc gccccggccg ccgcggcgaa caggtcggaa tagtcggaat
     1621 ccgcgccggc gacgggccgg agggactgga ccagcgccgc cgggatcggc cgggcggagg
     1681 ccgtgggccg cccctcggag ccggcgagca ttgccggcag ggccagcgct gcggcgagga
     1741 ggaggttggg cggcatggtc atcggcccgt cggggcgccg gcgccccggg ctagagcgcc
     1801 cgccagttcg actgcgccat ctccgttagg gctgggcgaa caggccgcct tccgatctcg
     1861 gctcgtcgcc gtggatccgg tcgagatagg cggcggcgag ctcgtaatgc gcctggacga
     1921 cccggcgatg cgccgcggcc tgggcgagct cgatctccgc ctcggctcgg cgctcataat
     1981 attctcgttc gtcgtgagac atttagcctg ggttcgaggt tggttacgga cgagtatcaa
     2041 aacggccggg gcgccgccgc cgttccccgg aatcgtgctg atccaggttg ttgatccttg
     2101 caaatgcgtg accggccggc cccggctttc cggtccaacc ggcacggcga ttccggccta
     2161 atcggcggcg acggcgtagc ggatgatcgt atcgtgctgt cccacgacca cggtcaccgg
     2221 ccgttcgacc ccgatcctca ccccctccgc ggtcaggtcg agcctctcga acctgtgttc
     2281 gccggcgccc aggccggcgc ggccgttcag gctcggtgcg gccgggacga ggatggtgta
     2341 gcggctggtc cggccgccct cgctcatgaa gggccggtcc ttcaccgaca gcgcgccgaa
     2401 ggggagggtg atctggacca gccgcggatc cgggaggacg agctgcgctt cgcccagcag
     2461 cgtcattccg cccggtccgc tcgcgaagcc catctcggtg ccgcgatcgc cgctctgcgc
     2521 cggcgccgtc acctcgaaca cgatcctgtg ggccccgagc gcgtagaatt cctcggcgct
     2581 cttgatctcc ttccgctcgg ccgcgcggag cttcggctcc agggccgcct tggtcgcggc
     2641 atagccgaag gcggagcgcg cgccctcggc cagcccgccg gggtcgcgga ccgagccgaa
     2701 gccggtgagc accgccagcc gctcgtcgag ggagtcggcc gattcgacgg ccgagcgcca
     2761 tcccttcgga tcgagatcgc gcagcaaata ggtgagggcg gcgccgacgc cgtagctgcg
     2821 cgaccggaac caggcggtca ggaagggctg ccccccggcg ccgagatcct cgcggagcac
     2881 ctcggcgatc aggcccgcaa ggttgccgtc cggctccgga ccgagcgcca ccgcggcgcg
     2941 gtcgatgaac ttcgcggttc cttcgacctt ctcgagctgc ccctccaccc tgaccacgcc
     3001 gggcggcagg ctcttctccc gctcccgcct cagcgcgaga tattggtgga gcagcccgtc
     3061 gcgcatcctg cctcgacgca tggtcagcgc cgaggcgagg atgcggcgtt cggtctcggc
     3121 gcccgcggcg aagctcaccc ggtccggcac cgccgtcgga gccacgaact gggagccgct
     3181 cggatgcctg aacgccttgc gctgataggc gtggaactgc tcgtggaaga tgagcgtcgc
     3241 caggtccttc gccgcctcgt cggcgaggac caggacggcg gtccggccgg ctccgatcgg
     3301 atagcccagg atgaagggtc ggccgacccc gtcgagattg cccggacgga accaggcgcg
     3361 gccgcggagg gccttcggca cccccgccgc gggcagcgcc gcgaaaggcg gcggcggcgc
     3421 atcggcggcg accagcagcg ctccctgccc ggggcggtag atgatgaagg cctggtccgg
     3481 aggccagaaa cccggccaga gcctggccag cttcggaccc gttctgtgta cctcggcggc
     3541 gagcttcagc agttcgggcg ccggcgccgt gacggggggc ggagcgcccc gcacctgggc
     3601 ggaagccgcg gccgccgcgc acaggagcat cgacgccgcc aggatcagtg cacgcatggc
     3661 ctcatcgtgg cctacatttc gtcggtccgc tagggcccgg cgccctcggc agcgtcccga
     3721 tcgtcccctc ggcgccgccg gcgcatccat tggcatttgt cccctttaga catgtagggg
     3781 ccgccaattc ggagttcagg aatgacgcac actagggcaa ggcgacacat ttgagctaca
     3841 caacccgttc gtctttttcg agttggtggg gcgggccgtg gctgatctgg gattctcagg
     3901 cgtcgtcgag aagttcgagg aacactgggg aaagcgtccg actcgctggc tattgctgat
     3961 tatagggctg ggcgtcgcgg cggtgtgcgt cggggcgatc tgggaatggc ttatctcgcc
     4021 gcttcttgca ttcattggct cgccccagcg gtcccagaca atctgggctc tggttctggc
     4081 gatcgttggc attggagctg ggatttctct gaccacgtcc ctcaccactg ccctggcccg
     4141 ctggcggcga atccgaacgt tgaaccagat agttagtgaa accgagacca aggcggatca
     4201 gatactcgac cgagccacag ggctggtgag caaagcctcc caagacaatc ttcagacgcg
     4261 agcccttttg gaggcgtcgc tattggtcac aaaggcgttg actgaaaagg acccgacgat
     4321 aggcgaggac gaacgggctg aaagattggg ggagattgaa cggggtttgc aggaattgca
     4381 ggaattgcgg gaaacgcagg aaatgcgaaa aaagagaggc gaagatggag aatgaacgta
     4441 ccttcgacgc cctcctaaag gcgattgttt cgggcgaggc tcctagctct cgaaagagaa
     4501 aagcatccgg cggagcatca ggtgaggaac agcacgcacg ttccagcgat actcaaactc
     4561 cccgcgatac ttcggagaaa gcttcccgct gacgtgaatg tgggtgccgt tgatcccgca
     4621 ctttagtagc gcccagaagt cctcaatcgc gttgaccccg cgccgctcgc gtcggcatag
     4681 tgccccgcct tgtgatccac cttgacgtgc tggaagccgc actggtccaa gtacttatac
     4741 ccgccgaact cgtcagtgtg gacggtcgtg ccgggagaac ctgagtgtga atgaggccgc
     4801 gcatggcttc cttcgtgcgg ctcccgacga cccgactgag cagctcgcct tcgcgttcca
     4861 gcatgccaac gatacaggtt ttgttgccct tgtagccctt ccccatgccg gagacggaac
     4921 cgaggtcggg cgcggtggcg ccgtcccggt cgtgccagac caggaacagc gtcatccgag
     4981 gccgctcagc ggatgcgcgg cccgccgaac gggaggggcg gcggcgggcg gcggtcgcgg
     5041 cgcgggatct gcgcctggta ggcgactccg caatgctcga cgcaataagg gaagccggga
     5101 ttggccggag cgccgcagaa atggaaatcg ggctcgccgg gatggccgat cggccatttg
     5161 cagatcttct cgttgaggtc gagcaggccg gtcttgtcgg cgatctccgg gctcggcttg
     5221 gccggcacca gccgccgcgg cggcgccggc gggatcggcg actgctgctc gccgggcgcc
     5281 tggcggacga agccgcccgg cccgatcgag cggatctgcg cctggggcgc ggccggggcg
     5341 gcggccggct cctgccgggc gtcgggctcg gcgtcggccc ggtcttcgtc cggctccgct
     5401 tcgctcgccg cttcgggctc gaagggggag gggccggcct cgctcccgcc ctcgttcggc
     5461 ttgaccggcg acggccgcga ctggaggccg aggcgatggg ccttgccgat caccgcgttg
     5521 cgcgagaccc cgcccagctc gtcggcgatc tggctcgccg tcatgccctg gctccagagc
     5581 tccttcagcc ggtcgatccg ctcgtcggtc caggacattt ggcgtctttc cttcatgctt
     5641 gcggcccacc gcgtctgcgc ctagcgacag cgcggtgaac gaccagcctt ctcattggat
     5701 gcgggccgat ccgggcgttc cggtgatccg gaacgtgaac tggggcggcc tcgggaccct
     5761 ctatgtgaag gaggtgaggc gtttcttcaa ggtccagctg cagacggtct gggcgccggc
     5821 ggtcaccacc ctcctgttcc tggtcatctt caccgtcgcc ctcggccgga gcggccgaac
     5881 cgtcgagctc gatggcgcga gcttccgctt cgccgacttc ctcgcgcccg gcctgatcgt
     5941 catgggcatg ctccagaacg ccttcgccaa ttcctccttc tcgctgctgg tgggcaagat
     6001 ccagggcaat atcgtcgact atctgatgcc gccgctctcc accgccgagc tgatcgcggc
     6061 cctggtcggc gccgccgtca cccgggcggt gctcgtcggc ttcgcggtct tcctcgcgat
     6121 gcagctctgg cccggcgtcg acgcgacgcc ggtcgagctc tgcgccgtcg cctggttcgg
     6181 gctgatgggc agcatgatgc tcgccttcct cggcctgatg acctcgatct gggccgagaa
     6241 gttcgaccat gccgcggcgg tcaccaactt cgtggtggcg ccgctctccc tgctctcggg
     6301 cacattctat tcgatcgaca gcctcgcgcc ggccttccgc acgatcagcc acgccaatcc
     6361 cttcttctac gtcatttcgg gcttccgcta cggcttcctc ggagcggcgg attcgccgat
     6421 cctggccggg gcgctgctcc tgctcgcaat caacctggtg ctcggcggtc tgtgctatgc
     6481 gatgctgcgc gccggctgga agctcaagaa ctaggcccgg caggagaaga tgcgatgtcg
     6541 acgacgctga tcctcgcggc cttcctctcg gccgcgccgg cggccgcgcc cgccgacgcc
     6601 cgctctccct accgggtcga ccgcagcctg agcgacgccg accggctgag cgagcttcgc
     6661 atcgacgtca cctccgccta cgacggcggc cggatcgatc gggcgacggc caccgaattc
     6721 tacctcgcca tagagcggat ccggcggcag atgatccgga tgggcatcca ggtcggctac
     6781 cgccagcgcg tccggctgcg cgcccggatc gacggcctct acgcgcgatg ggcgaagcgc
     6841 cagtcgctca acgccgaggt ccgttcagga aagtagggct ttcgatgaac gctggctgtc
     6901 ggccccgttc agccgccgaa aattcgccga gtcgtaggac cgccctacca agtgaggagc
     6961 aagccactcc ttgccagtga ggagcaagac catgcgtaag atcctgatgt ccgccctgat
     7021 gctttcgacc gtcgtcgccg tcgcccccgc cgccgcccaa tatggccatc gcgaccgcgg
     7081 ccatcacgaa tggaatcgcg gcggcccgtc gcgccaggcg atcaacgagc tgctccgcga
     7141 cctgcgccgc gccgagaacg gcatcgagcg gtcggtccag cgccgggtca tctcgccgcg
     7201 cgaggcgatc gggcttcgcc gcgaggccaa ccgcatcgag cgccggctca gcctggccgg
     7261 ccgcgacggc atcagccatc gcgagttcgc gtcgctgcgg atgcaggtca accggctcca
     7321 gcagcgcctg cgcatggagc gccgcgaccg cgacggccgc cgctactaaa ggcaggcaag
     7381 ggcgggcgcc gcgaattgac gcggcgcccg ctccctctct agaggcagga aggcgatccg
     7441 gtgagggtcg ccttttcaca ttccaatccg cggagccccc gtcatggcca tcacgcccct
     7501 catgcccgtc tatccgcgct cgccggtgcg gccggtgcgc ggggagggag tctggctctt
     7561 cggcgagggc ggcgagaaat atctcgattt cgccagcgga atcgccgtca atctgctcgg
     7621 ccacggccat ccccacctga ccaaggcgat ccaggagcag gcggcgaccc tgatccacgt
     7681 ctccaacctc tacggctcgc cgcagggcga ggcgttcgcg cagcggctga tcgacaacag
     7741 cttcgccgac acggtcttct tcaccaattc cggagccgag gccgtcgaat gctcgatcaa
     7801 gacggcccgg cgctaccacc atcatcacgg ccggcccgag aagcacgtcc tcatcacctt
     7861 ccgcaacgcc ttccacggcc ggacgatggc gacgatcagc gccacggacc agcccaagct
     7921 ccgcgacggc ttcgcgccga tgcttcccgg cttcaccgtc gtcgacttcg acgacctcga
     7981 cgccgccgag gccgcggtcg acgacagcac cgccggcttc ctggtcgagc cggtgcaggg
     8041 cgagggcgga atccgcccgg ccagcgcgga gttcatgcgc ggcctgaggc ggatctgcga
     8101 cgagagggac ctgatgctgg tcctcgacga ggtccagtgc ggcgtcggac gcaccggcaa
     8161 gctctacgcc tacgagcatt acggcatcga gcccgacatc ctcgcctccg ccaagggcat
     8221 tggcggcggg ttcccgatgg gcgcctgcct cgccaccgag aaggccgcgg cgggaatgct
     8281 ggtcggaacc cacccctcga cctatggcgg cggcccgctc gccatggccg ccggccaggc
     8341 cgtgctcgac atcgtcgccc ggcccgagtt cctcgagcgc gtcacccgca tgggcgagcg
     8401 gctgcgcggc gcgctcgagc agatgatccc caatcacgat catctgttcg agagcgtccg
     8461 ggggctcgga ctgatgctcg gggtgaagat gaagaccgac agccgcgcct tcgtcggcca
     8521 tctgcgcgag cacggcctgc tgaccgtcgc cgccggcgac aacgtcatgc gcgtgctgcc
     8581 gccgctgatc atcgaggagg agcatgtcac cgaattcgtc gagcggcttt cggaagcggc
     8641 gcggcactac gaggtgccgc aggcggcgta aagtcctccc catcgtcccg agggggagga
     8701 tgtcggagaa cttgatccgc ccccggacct tcgccacatc gcggccatga cttcccccga
     8761 cttccaggcc gaggcgcgcg ccgccaatct cgacgaggcg ctcggcttca ccatcccggc
     8821 gcgcaacgcc cgcggccggc tggtccggct cgggccgctg ctcggcgaca tcctgtcggc
     8881 gcatgattat ccggcgccga tcgcccgcat cctgtccgag gccctggtgc tctcggcgct
     8941 gctcggctcg ctcctcaagg aggcggaggg ccagctcacc atccaggcgc agaccgagag
     9001 cgggatcgtc gagctgctcg tcgtcgatta tcgcgccggc gagctcaggg gctacgtccg
     9061 cttcgatccg gaccggctcg gcgacctgcc cacccatcgc gacctcgccg ccctgttcgg
     9121 caagggctat ctcgccatca ccttcgacca tcccaaggcg gaggagcgct accaggggat
     9181 cgtgccgctc gaaggcgcct cgctcggcga ggcggcgcag aactatttca gccagtccga
     9241 gcagcttccg agcgtcgtcc ggctcgccgt cgacgacacc ggccacatcg ccggcgggat
     9301 cctcgtccag cacctgccgg aaggcgagga gggcagggac cgcctccaca cccgcctcga
     9361 cgatccggaa tgggagcatg tccggctgct cgccgagacg atcaagggcc atgagctgac
     9421 cgacccgagc ctcccgctcg gcgaagcgct gtggaggctg ttccacgagg aggaggtccg
     9481 ggtcctggcc gccgtccccc tccgcaaggg ctgccgctgc aatctcgact atgtgcgcgg
     9541 agtcatcgcc cggttcggcg ccgccgagcg cgccgagatg gtcgacgagg acggcttgat
     9601 cagcgtcgac tgcgaattct gctccaggat cttcccgatc ccgctcagcg acttcgagga
     9661 ttgaggcgag gcggcccaca gccgggtcgc atccttcggc atttgctgct agaagccccc
     9721 gtatatgtcg cagttgcggg gtcgtttgcg ccggggggcg ttggggctgg tcggcgggtt
     9781 ggccgtcgcc gcgcccgtcc tcgcgctcag cctgcccagc ctcgaggcgc tcgcgaagct
     9841 cgagcgcggg cgctggcgga tcagcgaatc gcagagcggc gccgcccgct cgctctgcct
     9901 cgccgatccc gcggaattcc tccagctcga gcatcgcggc gtcgcctgca gccgggaggt
     9961 gctggccagc gaggccggct cggtcacggt ccaatatacc tgcccgggcc gcggcttcgg
    10021 ccacaccagc gtccgggtcg agactccccg cctggcgcgc atcgacaccc aagggctggc
    10081 agacggccgg cccttctcct accgcgccga ggcgcgcaag gtgggcagct gctgagggat
    10141 ttgagggccg gccgccttct cgctagttct tggcggagca gcggatccac gccttctgga
    10201 caaacgcggg aagctcgccc cgcgattggg tcttaagcag gccgtcgagg gccgaagatc
    10261 gagcttcggg cttgtccctg agaatcgacc gataggcgtt gagcgcccct tccaggcccg
    10321 ccagctgctg ggtattcgga tcgttcgctt tctccggatg ctcgatcgcg agggcggcca
    10381 ttgcgaagct attctgcagg ataatctctc cggcatagcg ataattgctc ttgaccaggc
    10441 tgcccagcgc gtccgcgcaa atggtcacgg tgacgtcggg ggcgtttgtg agccatgtca
    10501 gcgcccattc gcgatccgcc ttgagggctg gctcgagcgg agcttgttcc aggttgcgcg
    10561 tgacggagac gaagcgccgg agatcctcgg gcgtcgaacg ctcgtcccag gccgacgccg
    10621 gcgtcagtcc gcagatcaga gccaggccca gcgccaaccg ggcggtcagg cgagaggcga
    10681 accggggtgt gatcgtgctg ttcgacattg aagcctcctg gtccacgcct attgtagcgg
    10741 gcttgcggag caatgccacg atcgtcgtcc tccgcttcgc ggcctgggtt gcagctcggc
    10801 ccgttaaccc cctgtttacc ctgatccatg taaagaggat tcgtgccgat tgtgggcacg
    10861 ctttcctccc tagcaggctt aacagcctaa actgggccgc tcctcaccgg gcggcccgtt
    10921 cttttgcgcc gcttgcgccc gccgtcgcgg cggattagcc gcgccggtca tgtccacgcc
    10981 cgttcgcaag gccgtcgtcc tcgtctcggg cgggctcgac tcgatggtcg cggcggcgct
    11041 cgcgcgcgag tcgggcttcg ctctgcttgc cctcaccgtc gactacggcc agcgccaccg
    11101 gatcgagctc gaagcggccg cccgaatcgc cgccgccctg ggcgccgagc gtcacgtcct
    11161 cctcccgctc gacctccgcc cgttcggcgg ctcggcgctc accgccgaca tcgacgtgcc
    11221 gaaaggcggg gtcgggcccg acatcccggt cacctacgtg ccggcgcgca acaccatctt
    11281 cctgtcgctc gccctgggct gggccgaggc ggcgggctcg gccgacatct acatcggggt
    11341 caatgcgctc gattattcgg gctatccgga ctgccggccg gacttcatcg ccgccttcga
    11401 gcggctcgcg tcgctcgcga ccaaggccgg cgacgaaggc ggctcgttcc ggatcgccgc
    11461 gcctctcctg cgcatgacca aggccgacat cgtgcgcgag gcggaccggc tcggcctcga
    11521 ccccggcctc agctggtcct gctacgaccc ggcgcccggc ggccgccatt gcggactgtg
    11581 cgacagctgc cggctgcgcg cccgcggctt cgccgaggcc ggcctcgccg atccgacccg
    11641 ctacgcggcc aggccctgag acctatttcg cggtcccgcc gcaggcgcgg cggagcacgc
    11701 ccttcttgtt gaagcattcc ggcggcggcg ccagcgacat gtcccggaac cggatgagat
    11761 ggggcagata ggccggcctc ggatcgtagg cggggacgag cgcgttgcgc agctcgcgca
    11821 tccgggcctg ggcgagggga agcagctggc ccttgggcgt cgccagcgcc gacttggcga
    11881 tgttggtcgc ggtctggcag aagatcatct gcgcctgcag cgtcgaccag ttgttgtagg
    11941 tgatcgtcga atagtcgtcg aacgccttct ggcccttcgc gccctgcacg cgcttgaagt
    12001 agttgttgag cgtcgtgtag gcggccgcga gctcgacatt gtggtgggcg agcaggccgt
    12061 tgtagttcgg cacggcgcgc agatagtcgg agaactggca ctggagcgcg gcgacgttga
    12121 gccccgcgcg caggttccac agcaggttgg cgcgatgctc ggcgggggtc gctccgggca
    12181 ggggcaggcc gaccagcggg tcgctcggct cgatcgccga ggggcgcaga ttgggctggc
    12241 tgatgtagag ctgggccggg gcgggcgcgg cggcgagcgc cgcggcggcg gccgccatcg
    12301 agccgtactt cgtcctgaag gtcattcgat cccctcgagc gctggacacg ctggttccat
    12361 gcttagcagc ggccgcctct caataaagct tcacccgccg tgcccatcgc gcctgcggca
    12421 cggctcgtca ctcctaagcg cctgcccccc ataagaaaag atgccacgga cgagaaaagg
    12481 gccgcccggt cgcccggacg gccctttccg cgaaaacggt cgaccgtcga ttacatgccg
    12541 gtggtgttgc cagtcccggt gttcgtcgtc gtgtcggtgg tcgtcgtgtt ggtcgtcgtc
    12601 gtcgtcgaca cgttggccgc gtcggtcgtc acgccgagat tggcgtccgt gccgttcaga
    12661 gcgtcgacgg gagtgacgtc gttgccgccc atcagggggt cttcgacata gttggtggcg
    12721 gtgttgttgg ccgccgcatc ctcgccgccg ccgcaagccg cgacgagcag ggccgcaccg
    12781 gcaaccatcg aaccggtcat cactttcgaa agaaggtcac gcatttagaa actccctagg
    12841 ttggcgattt ggtcggggtg gcccctcttt tgccaaatca gttttgcaca ataaacggaa
    12901 aggatagtgc tctcaagcct tactcgcgtc ccccctccag ggagcgcagg aaatcgcaga
    12961 gcccttccct cagcgccagg tcgaaacggt cttgtccggc agacgctccc atctcgtcga
    13021 ggctggtcac tgggaagtcg gcaataccgc agggcactat gcctgagaag tgggaaagtt
    13081 gcgggctcag attgaccgag aatccgtgat aggtgaccca gcggcggacc ctaaccccga
    13141 tcgctccgac cttggcctcg ccgcccggcc cgtcggccca gatcccaatc cgcccctccg
    13201 cccgccgggc ctcgatcccg agccgcgcca aggtcgcgat cacccagcct tcgaggcacc
    13261 agacgtaatg gcgcacgtcg cgcccgcgcc gctcgaggtc gaggaggacg tagccgaccc
    13321 gctggcccgg cccgtgatag gtgtagcggc cgccacgccc ggcctcgtag acgggaaagc
    13381 cctgcgggtt gaacaattcg gccgggtcgg cgctcgtccc ggcggtgtag aggggagggt
    13441 gctcgagcag ccagacgagc tcggaggcct gcccggaccg gatcgccgcg gcgcgcgcct
    13501 ccatcgccgc gagggtctcc ggataggggc tcagccccgg cgtcacccgc cactcgattt
    13561 cgtcatcgtc cgccatcgcg gaccttcctt cgccgcgagg gaagggaagg gcaagggggg
    13621 cgcgggcgtc gggcttgctc cgtcccgcct gtgccgcccc cgtcatcctg aactcgtttc
    13681 agcatccagg cccgaaccgc cgcgggaccg gccgcatgct tcccgcgact tccccctcac
    13741 ggccggcagg acctgtcttt gagaaatggg gcaggtaacc ctgcctgtct cctgacatcg
    13801 ggtcgtggct ttccggactt gcccgttcgg gatgacgact gagatcgggt tcacgcaaag
    13861 acgcgaagcc gcgagaagcg aggcgcgaag gcgccgaggc tggcattgag ggcggcatga
    13921 tccgccaaaa ttgtgctacg gatggctgca tggacgagaa gctcttgaat ctgccttggg
    13981 aggtccagct tgcattggct agcggctacg ctgcctacat gctggcgtat gtaggtatca
    14041 gggagcacca caaggcgatc gacgtcacgt tccgcaccct cgcgttcggc ctttgcgcga
    14101 ccgctgttct cgccctctcg ccatcgcaac taggctggcc gagagcagtc gcggccgtcc
    14161 tcgttgcact catgggcgga gtgctttggc gcttctggct cgcagagggg atgcaacgtg
    14221 ccatccggaa cgttaatctg tcttggtcag atgatacgcc gagcgcgtgg tcacgagtta
    14281 cccagcacaa cagtcgacac tttgtgacgc aggtgacagt ccaccttgac gacgacagtt
    14341 ggctcttctg cgatgcgacc ggcagtttcg gagccgcgcc atttggcccc tgcatcttcg
    14401 ggacgaacgg cgacattgcg ctctacgtca ctcactcggc agcgccgggc gaggacttca
    14461 aggcggtcgg aaacgcggtt cgggatactc atcacggaga cgagcttact tgggtgccgg
    14521 catctcgaat ccggaaagtg tcggtgcgtc taaaggggtc cgttaacgct tcgtcggcgg
    14581 aggcggggca ggcgcaggcg caggcgcagg cggtggaggc ggtgccttcg gccagcccga
    14641 gcgccgttcc caatccggcg gagtagggcg gctacccatc acttccggct cggcgcggga
    14701 gggggcgggg caggcctagg cgaggggggc ggggccttcg gctgacttga tgtgcccggg
    14761 ttcgggtgtt cggtcggcat ctttccttac ctctctcgtg tgcattatcg tagaaaaaca
    14821 gtgacagtat acattttcca gccagacagg cagtcttgaa gacagaaaat gcatactgcc
    14881 accagtttcc tagggggcga aggtgacgat ggacatcgca tggttcctga acgagcgtct
    14941 cgcgttcctc cgagtccatt tcgatatctg cgtgtcagcc ttcgaggagc ggaagcggaa
    15001 gattgaagcg ggtgaaccac cgttcaacaa cccacccatt gatgaagatg gcgagccgcc
    15061 tttccttcag gaatggatcg acgcccagac ctcgattgag ctcatcggcg tagcttgcct
    15121 atccctgcta agcgatgcgt ttaagctcta cctaaatctg ctccaacatg accagctgca
    15181 tttcgtgttc aacgacgacg aaaaacgacg ccttaaaagc gaaggcttcg tccccgcgta
    15241 tcgagacgcc ttgagtgaga ttttcgacac agactggcag gcgagcggga tcgatttcgc
    15301 ggtaatcgaa caggttgtgc tggctcgaaa ccgagggcag cacgggaccg ccctcaccag
    15361 tttcatgctg acccacgatg atcgcacgat tgccaagcat ccccgtcccc tttttctccg
    15421 cgctgacgag gcaagagcac tcgataccgc agcagtgcgt atgggagggt tcctcaatcc
    15481 gttgctcgtt gtgacacgtg aaacgttatt tgcggcgatc gaagaggtgg agaaattagc
    15541 tcgctggatt gacgccaaca tggatcgggc tttgcgctgg cagattgagc aacgatcctt
    15601 aaaatcgagc taagagcaat tcagagagca atcagcgaca gacatttata gatagatgcc
    15661 ggtcaccagc gcggtagtaa tgtatactgc cacatttttc tccactaaaa ctcccaaccc
    15721 tcacgctcca tgaacctctc gagggagaga cagtcgacgc cgaaatgagc gcagatgttc
    15781 ggaattttgg cgccgttttc cttaaactgc tccatggtaa ccacacacct tccttcggtg
    15841 gcggccttgg cgatcacgaa ggggtcagcg ttccggccac ctttcaggag cttcttctgt
    15901 tcgatgttct gctggaaatg ccgaacggca tagatgcgcc ccacgaatga gccctcggcc
    15961 gccgtgggca cgtggaacac ggactgatgt gcggtaaccc agtcgcggag attctcgaag
    16021 gaactatcca cggcctcccg gtacacctcg cgggtcgaga cgattcgccc gtccgcaact
    16081 aatgcgtcga accggtccca cagggtagga aagcgacggc gatagtagtt gcggaagagc
    16141 actgagatgg gggagttgtc gaacacgtac atcagacatt cccccgagcg aagtagtctt
    16201 cgagggtgcc gacattcttg ggcttagtgt cgaggtaatc ggcaagttgg ttctcgtcga
    16261 ttcggttctg gtggaactgg cttagtgcca acgccacgta ttcgcggccc aggtatgcaa
    16321 gcttcgtcca gtagtggttg ccgccgactc caccctcccc ggcacccgct tggcgttggg
    16381 ccttccaccg ctctaccgct tcaaggtact gcgattcacc tatccggccc tggtcgagga
    16441 accgcctgaa caccacctcc ctgctaacgt ggaactgtga ggcgacaagc tccgcggttg
    16501 cttcggtcgc aggatggccg gcgacggccc ttgcgaacgc ctcctctggc agcaggaact
    16561 gcgccgcgaa gccgttgcag agtatctcga tctgtttcga gtcgcctgcc agccgctgaa
    16621 tgtagcggtc gcgcactttg tcgatgccgc tggtgtggaa gatgagatgg gccagctcgt
    16681 ggaagtaggt gaaaatctgg cgcgtctttg ctgagctgtt gttcacatag atgataggga
    16741 attcaacatc gtagaggcag aaccccgaaa gctcgtcggc tcgaaacgca tccttgaaca
    16801 cgaaaatgcc gacccgctgg agcgccgtcc gccactcctt gagcgcggcc tcgtcgtcct
    16861 tccatgccgt ttgctgctca agtgagacgc cgatgtactc gcggaccgcc ttggtcatct
    16921 cgacgaggtt gacgttcggg ccgaattgca ggtcgcgggt gataaggcgc tcggcagggt
    16981 tacggccctg ggtaagctct gcaaggctga gctgcattgc ctttgctttc ctcatcagca
    17041 ggcggatgcg actcggcagc cggtccagct ctgcctcggg cagggtccga aacgtctcgt
    17101 taatgggagg aacgttcggt ggctccggga agaagaagac agctatcggg actttgaacg
    17161 cgtccgctag ctgctccagc tgcgggtaag tcggcgctgc ttcgccctgt tcccactcag
    17221 cgaatcgctt gaagcgctca ctggcctcgt cacggctcaa tcctgcgcgc tcacgcgccc
    17281 aaatcagaac ggcggggtta atgggaatct gctcggtcat gtggcctaga tatagccgag
    17341 cgcgccagcc atcctactgc caatgaggtt ggctgcttgc tagaggctta tgtgctgagt
    17401 gcacgtcgat cataggggct gtgatatcgc tactctcggc tcgcaccccg cgcccagcgt
    17461 cccgctgaaa atgtataccg tccggtttcc agaaaatcgg tttcccgcga cgaaaacccg
    17521 cgcaggaaat tctatctcgc ctgcttcttt tgaggggcca ccccaatcag ccgcctgatc
    17581 gcctccggcc tggacgggtt cgggtcaggc tgagcctcga tccaggagtc gagctccgcc
    17641 tgcatctccg gatagaggcg aacgctgatc gtctgaaggt gggcgcgggg gctgtccaag
    17701 ggaccgcgtt tctgatatgt tccacctagc cgcacggcct ctcccgaggc tgccaggaac
    17761 gcggcgagag gagggtggcg ccggtggccg gcgaccgacc gatcgggctt ccgggagcga
    17821 cggggccaag tccttggccg gcaaggattc tcgccgcgaa gttcacccaa gggccgcccg
    17881 actcgatgaa catcatgaac tttgccggaa aaaggcttgg ctttcagcgc tcgcggcctc
    17941 tggcgccacc gggggaaccg gccttccagg ggtgccgctg ccgcgtcgca agcgctcctt
    18001 gccggcgaag gcggacaagc ccgcgcttga ggcggcccgg cgcggtccct atgctgctgc
    18061 ggcatcaaca gggggcgagc gagccgatga aattctccta cagcgccgtc tgggccgacg
    18121 tcatggccct gctgcggacc cacgcctcgc tgatcgcgac cgtggccggc gtgttcatct
    18181 tcctgcccgg gctgctggtc gcctatttcc tgccccagcc ccagacccag gagctcgccc
    18241 ggatcggcga ggtctgggtc gaatatgcca ggctcaactg gccgtggatg ctgctcaaca
    18301 gcctcttcaa catcgtcggc tcgatcgcca tcctgcgcct gatcttcgag cgcggggtga
    18361 cggtgggcgc cgcgatcgcc ggcgcgctcg ccctgttcgt gccctatttc ctgacctcgc
    18421 tgctgagctc ggtcctgatc ggcctcggct tcatcctcct gatcctaccg ggcgcctatc
    18481 tgctcgggcg gctcagcccg gtcaccgcca tcgtcgtcgc cgagcggcga cgcaacccga
    18541 tcgacgccct caagcgctgc ttcgagctga ccaagggcca tggctgggcc gtgctcggcc
    18601 tcctgctcct cgtcgccatc cccgcgtcga tcgcgatccg ggtcgccacc atgctgctcg
    18661 ggatcctgtt cgtcctcgtc gccggccagg atctgggaga cctgctggtc ctgatcgtcc
    18721 ggaccgccgg cgccgccgcc ctcgccgcgc tcatgctggt tctcaacgcc gccatctacc
    18781 ggcagctgag ccagtcgagg ccggaagccg ccgccgccgc cgccgcggat ccggggcctt
    18841 gagccgagcc gcgggttcag ccggccccgc cgccgatcga gggcagatgc ggggccgcgt
    18901 cgcgcgggag gaggccgttc agccgcgggt cggggaagct ctcgaaggcg cgctcgtagg
    18961 ccatgaagcc ggccttgaga taggcgggga gggcggcggg atggtcgagc gtgcaggtgt
    19021 tgacccggac ccgctcgacg ccgggccgcc aggccagcgc cagggtctgg gcgaacagcc
    19081 agcggccatg gcccctgccg gcgagctcgg gcacgaggcc gaggaagcgg atcaggcaat
    19141 cgccgggggt ccgcaggtcg agctcgatca gcccgacctc gatcccggcc gggtcgagga
    19201 cggcatggat ctcggccatt tcccgccgca gccgcgcatc gtccatgacg aggcgcgaat
    19261 accagagcca ccgctcgccg accttgcgga acagggctcg gtagcgggcg ggatcggagg
    19321 ccggccaggg ctcgagtcgc aaaggagagg cgggcagggg gccaggcgga ggccgccggg
    19381 tcatctccag gtaggtgacg acggcgccga catggccttc ggggagcggg gtcagcatgc
    19441 cgattcggat gccgggacgc gcggcggctt gcaagccggc gaagccgccc tcggggggag
    19501 ccgagtcgcg ggaggccggc tagcggcgcg cttccgcttc ctcgatccgt cggccggcct
    19561 gctccgcctc ggcgcaatgc cggcccgatt gccggcagct cgcctccagc gcccgaagca
    19621 cgcggcgctg ctgctcgatg acatagggat cgctcgcgac cctgggagaa tagacgtcgc
    19681 gatgggccgg ggtgcgggtg gcgggcggcc gggcccggct cgccggccgg gccggttcga
    19741 ccgacggcgc gccgccgccg ccgatgagga gtcccagcgg gaaggcgatc gacgccgcca
    19801 gggccgccgc gcagccgatc gcccgccagt cccggccggc cctcccggtc atcgggggag
    19861 ggcctgagcg aggaggagca ggtagaggtg gaaggcggcg ccgaaggcga ggatcgccag
    19921 cgcgaggagg aggcagaggg ccgccggcgc ccgaatcgcg gacaggggaa cgacggtcac
    19981 cggagggtcc cgccgctccg ctcgggacgg gcccgcggag gaattcgcgg catcaggcgc
    20041 tcgccgagcc ggccgtgaag acggcgagct gcgcgtcgaa ggcgcgccgg taggccgggc
    20101 gcgcctcgcc tcgggcgatg taggcgcaca ggcccggata gccgtccagc aggcccgagc
    20161 cctccagcct tcgaagcacc gtcaccatca gcaggtcgcc ggcgctgaac ccgccgtcga
    20221 gccagtccga gccgccgagc cgctcggaga gctcgtccag gcggcggcgg gcccggtcgc
    20281 gcaggagcgg caggcgctcg gcgtgccagg gcctgtcgcc ttccagatgc tcggcggcct
    20341 cgaggtcgac gatcggcggc tccatcgagt tgagggcggc gaacatccag gcgatcgccc
    20401 gcgcccgggc gtcggcgtcg tccggaagca ggccgcgatg gcgctcgccg acgtggagga
    20461 cgatcgcccc cgattcgaac aggacgaggc cgccttcctc ataggtcggg atctgtccga
    20521 aaggatgcag ccggcggtgc tcgggccgct tcatcgcctc gaacgagacg aggcggacgt
    20581 cgtagggctg acccacctcc tcgagcgccc agcggaccgg catgtcccgc gccagcccgc
    20641 ggccttggtc cggggagcgt tcgaaagcgg tgatggtgat cgtcatccct gtctccccga
    20701 atcgcgccgc acgatgctcc gcgcggcggt gctcgggcaa cggggagtcg acatatcgcg
    20761 ttggatttcg cccgcgacag ccgctaggcc gggcccgggc gggccgcggg ttactgggag
    20821 ttgaaattgg acaagcctct ggtcgacggt ccggtcggcg cgtcggtgcc cgcggacgcc
    20881 gcgggccgag cgtcggttcg ggaaaacgaa gccctcttcc ggtttctcga tgcgctcggc
    20941 agggcgaccg ccgaatccgc ggacgcggac gaggtcttgt cgatcacggc gaggatgacc
    21001 gccgagcatc tgggactgtc gaactgcgcc tatgccgaca tggacgagga cggcgacggc
    21061 ttcacgatcc gcggcgactg gcacgcgccc ggctcgccct cgatcgtcgg ccgctatcgc
    21121 ctggcggact tcggcaagct cgcggtccag gagctcaagg ccgggcgccc tttgatcatc
    21181 aacgacaatc tgcgcgagat cgcgcccgag gaggccaagg ccttccagga catcggaatc
    21241 gccgccacga tctgcatgcc gctggtgaaa tcgggccggc tggtggcgct gatggcgatc
    21301 caccaccggg tcccccacca gtggacggtc tatgagctga cggcggtccg cgaagtgacg
    21361 gagcggtcct ggacgcatgt cgagcgggtc cgctcggacg ccgagcttcg agccagcaac
    21421 gcgcgcttcc gggccgcgat ccgggccacc gaaggcgtcg tctggaccaa cgacgcccaa
    21481 ggtcggatga tcggcgatca accgggctgg tccgccctga ccggccagag cttcgacgaa
    21541 tatcagggct tcggctggtc cgaggcggtc cacccggacg accgcgagcc cagcgtcgcg
    21601 gcgtggaacg cggcggtcgc ggcacggcgc gccttcgtct tcgagcatcg ggtgaggcgc
    21661 gccgacgggc gctggggcct gttttcgatc cgcgccgtcc cggtcctcgg cgatcagggc
    21721 gaggtgaccg agtgggtcgg ggtccatacc gacatcacgt cgcagcgcgc cggcgaggag
    21781 gcgcttcgcg aaggcagcca gaccctcgag atactcaacc ggaccggcgc cgcgatcgcc
    21841 gccgagctgg acctggagcg gctggtccag atggtgaccg acgccggcgt cgagctgacc
    21901 ggcgcccggt tcggcgcctt cttctacaac gtgctcgacg acgagggcgg aagcctcatg
    21961 ctctacaccc tctcgggcgc ggagcggtct gccttcgaac gatttcccca tccccgcgcg
    22021 accccgatct tctccccgac cttcaagggc gagcgggtgg tgagatcggc ggacatcacc
    22081 gccgacccct catacggcct caatccgccc tacaagggca tgccggaagg ccacctgccg
    22141 gtgcgcagct atctggccgt cccggtgacc tcgcgcacgg gcgaggccat aggcggactg
    22201 ttcttcgggc acgaggaccg ggacgtcttc agcgaccgct ccgaacggat catggtcggc
    22261 ctcgccgctc aggccagcat cgccatcgac aatgcgcgcc tcttccaggc cgtgcagcgg
    22321 gccaaccaga cgctcgagga gcgagtccag gaacgaacgg ccgagctcga gcaggcgcac
    22381 gaggccctgc gccaggcgca gaagatggag gcgatcggcc agctcaccgg cggcatcgcc
    22441 cacgacttca acaacctgct cacggtcatc cggggctcgg ccgacctgct tcgccgctcg
    22501 gggctcagcg aggagaagcg ccgccgctac gtcgcggcca tctcggacac ggcggacagg
    22561 gcggccaagc tcaccggaca gctcctggcc ttcgcgcgac gccaggcgtt gaagccggag
    22621 gtgttcgatg cggcgatccg ggtccacggc atctcggaca tgctccggac cgtgctcggc
    22681 tcccgggtcc gcctgaggat cgaggacgac tgcccggact gcttcgtcga ggcggacgtc
    22741 ggccagttcg aaaccgcgct ggtcaacatg gccgtgaacg cccgcgacgc gatggacggc
    22801 gagggcgatc tcaccatctc cgtcggaccg atcgaggatt cgaagggcag gggaatggtc
    22861 gcggtccggg tctccgatac cggccacggc atcccgcgcg accggctgga gcatattttc
    22921 gagcccttct tcaccaccaa ggaggtcggc aagggcaccg gcctgggcct cagccaggtc
    22981 tatggcttca ccaagcaatc cgacggcgag atcaccgtcg acagcgaggt cggcgcgggc
    23041 acgaccttca cgctctgcct gcccaaggcc gaggtcggac acgcgccccg gctggccggt
    23101 cgcggcgcga tcgatccggc cccgggcgga gggctggtgc tcgtcgtcga agacaatgcc
    23161 gacgtcgggc tattcgcctc gcagatgctc ggcgagctcg gcttcgcgac gagcctggcc
    23221 gcgaacgccc gggaggcgat cgggctgctg gaggcgcggg ccgaggaatt cgacatcgtg
    23281 ttcagcgacg tcgtcatgcc gggcctgagc ggaatcgagt tcgctcggct cgtgcgcgag
    23341 cggtggagcc acctgcccgt ggtgctgacc agcggctaca gccacgtgct ggtcgaggaa
    23401 tcatgccacg agttcccgct gcttcataag ccctattcgg tcgaggcctt gtcccggatc
    23461 ctggcggagg ctcgcctcag cgcaccggcc cggggccgat agcggccgac ctggccgccg
    23521 cgcgctcttc cgggtccagg ccgcgccggg aggaatcgat cacctccagg tcgcgagggg
    23581 gggcaggctc atcaggatcg cgtcgacatt gccgccggtg cgaaggccga acagggtgcc
    23641 gcggtcgtag aggaggttga actcggcgta gcggccccgc cattcgagct gcctcgcctt
    23701 gtccgcctcg gtgaagggcg tgttcatccg gcgtcggacc agcttcggga agacgtcgag
    23761 cagggcctcc ccgacgtcgc gggtgaaggc gaagctcgcc tcgaacgggc cttcgagatg
    23821 gtcgtagaag atgcctccgg cgccgcgatg gacgccgcgg tgggggatcc agaaatattc
    23881 gtccgcccat ttccggaagc ggtcgtagaa ggtctggtcg tgggcggcgc aggcggcgcg
    23941 cagccgagcg tggaaatcgg ccgtgtcttc ctcgtagggg atgggcgggt tgaggtcggc
    24001 gccgccgccg aaccagcgct tcgtggtggc gaggaagcgg gtgttcatat ggaccgccgg
    24061 cacatggggg ttggccatgt gggcgacgag gctgatcccg gttgcgaaga agcgcggatc
    24121 ctcgccggcg ccgtggatgg tggcggcgaa ctcgtcggtg aagcggccgg cgacggtgga
    24181 gacgttcacc ccgaccttct cgaacacctt gcccttcatc agccctcgga ctcccccgcc
    24241 gcccggcgct ccgtccgggt cgcggcgctc ccagggcgtg tagtcgaagg ccgcctcgcc
    24301 gccggcctcc cgctcgatcg cctcgaactc ggcgcagatc cggtcgcgca ggctctcgaa
    24361 ccagtcgcgg gcgcggcttt gctcttcgtc cagctcgatc gtcatggggg cccgatgctc
    24421 ccgggccggt caagttgcaa gacggcggct agcgcggacc gcctgccgag gagatatatc
    24481 acgggtggat tttcaggcgc cagaacaacc gggcggacgc gccggctcag acggcccaca
    24541 ggcccgtctg ccggatcgcc tcggcaagcc cgatcccggc ggccaccgcg aggttgagcg
    24601 agcgcgtccc ggaggtctgc gggatccgga cccggaggtc ggcgcggcga tggaccgcct
    24661 ccggagcgcc tcggctctcg gagccgaaca ggagggtgtc ggcgggttgg aagcgcgcct
    24721 cggggagggg gagggcgccg gcggtggtga acaggacgag gcgggcggga agcgcccgct
    24781 cgaaagcgtc ccaccccgaa tgccgggtca ccgcggccat ctgagcataa tccatgcccg
    24841 cgcgcttcag cgcccggtcg gagaaggg
//
LOCUS       DASVQZ010000302         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_928870, whole genome shotgun sequence.
ACCESSION   DASVQZ010000302 DASVQZ010000000
VERSION     DASVQZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928870"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VF547_08350"
     CDS             complement(<1..722)
                     /locus_tag="VF547_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262870.1"
                     /translation="MASETASETAAPRFRLRLPRRVPDFGPRGFFVFRIVWYAAFALA
                     LLGPVAGTWLRLHEGGRNSALVAGSRAGMALADQDLTLVRFPIGPAAAGIRPGDRIVA
                     IDTIPVSPGVRLPSPADPDGAGTETDQLLLGDLIGGSEDRDVLLRLRAPDGSERDVAV
                     RTGEGHIEQAARARGIPPRLLSFIDLIHLLTYPFLLVSAWLLYKRKRFDVVSSTVSLA
                     ILLTMGTEQPSAAFLASLDRLPG"
     gene            complement(814..1866)
                     /locus_tag="VF547_08355"
     CDS             complement(814..1866)
                     /locus_tag="VF547_08355"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX8262871.1"
                     /translation="MTVRAYGARAADRPLEPMAIERRRPGPRDVEIDIAYCGVCHSDL
                     HTVRSEWGGTVYPCVPGHEIVGRVGAVGGEVGGFAPGDLVGIGCLVDSCRRCPSCAEG
                     LEQYCENGFVATYNSPTADPPGHTLGGYSQRIVVDERFVLRIRHPEAQLAAVAPLLCA
                     GITTWSPLRHWKAGPGKKVGIVGIGGLGHMGVKLAHALGAHTVAFTTSPSKRQDALDL
                     GADEVVVSRDPQAMAAQARSFDFILDTVAASHDLDAFTQLLKRDGTLCLVGVPEHAHP
                     SPNVGALIFGRRALAGSLIGGLPETQEMLDFCATNGIVAEIEMIRADAIDEAYDRMQR
                     SDVKYRFVIDNASLAP"
     gene            complement(1990..>2431)
                     /locus_tag="VF547_08360"
     CDS             complement(1990..>2431)
                     /locus_tag="VF547_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262872.1"
                     /translation="VVTSTTDNVGDDAGTTETEVTTGTATTHGTTTTHGTTTHGTMTH
                     GTMTHGTMTHGTAVAANVTPPMSPVVHPSNAAPERDARGIPVISDPAVVPAGFNGTTG
                     TGVGGPMVDPATGATATGADASHPACSRTVTDNCVQTYERSRRR"
BASE COUNT          344 a          783 c          956 g          348 t
ORIGIN      
        1 ccgggaaggc ggtcgaggct tgccaggaag gcggccgagg gctgctcggt gcccatggtg
       61 aggaggatgg cgagcgagac cgtcgagctc accacgtcga agcgcttgcg cttgtagagg
      121 agccaggccg agacgagcag gaaggggtag gtgaggaggt ggatgaggtc gatgaagctg
      181 agcaggcgcg gcgggatgcc gcgggcgcgc gcggcctgct cgatatggcc ctcgccggtg
      241 cgcacggcga cgtcgcgctc gcttccgtcc ggcgctcgga gccgaagcag cacgtcccgg
      301 tcctcgcttc cgccgatcag gtcgccgagc agcaactggt ccgtctcggt gccggcgccg
      361 tccggatcgg ccggcgaggg caggcggacg cccggggaga ccgggatggt gtcgatcgcg
      421 acgatccggt cgcccggccg gatccccgcc gcggccgggc cgatcgggaa gcggacgagg
      481 gtgaggtcct ggtcggccag agccatgccc gcccggctgc cggcgacgag cgcgctgttg
      541 cgcccgccct cgtggagcct cagccaggtg cccgcgaccg ggccgagcaa ggcgagcgcg
      601 aaggcggcgt accagacgat ccggaagacg aagaagccgc gcggcccgaa atcgggcacg
      661 cgccggggca ggcgcagccg gaagcggggc gctgcggtct cggacgcggt ttccgatgcc
      721 atgtttgcga aagcgcccta cgctgccgac gaccccgccg tgcgatagca tgggccggcg
      781 cggcggccat cggaaaatcg ccgccgccgg ggatcagggg gcgagcgagg cgttgtcgat
      841 gacgaagcga tatttgacgt cgctgcgctg catccggtca taggcctcgt cgatcgcatc
      901 cgctcggatc atctcgatct cggcgacgat gccgttcgtg gcgcagaaat cgagcatctc
      961 ctgggtttcg ggaaggcccc cgatcagcga ccctgcgagc gcccggcggc cgaagatcag
     1021 cgctcccacg ttgggcgacg ggtgggcatg ctccggcact ccgacgaggc acaaggtgcc
     1081 gtcgcgcttg agcagctggg tgaaggcgtc gagatcgtgg ctggcggcga cggtgtcgag
     1141 gatgaagtcg aagcttcggg cctgcgcggc catggcttgc gggtcgcgcg agaccacgac
     1201 ctcgtcggcg cccaggtcga gggcatcctg gcgcttgctc ggcgaggtcg tgaaggcgac
     1261 ggtatgcgcg cccagggcgt gggcgagctt gacgcccata tggccgagcc cgccgatgcc
     1321 gacgatcccg accttcttgc cgggccccgc cttccagtga cgcagaggcg accaggtggt
     1381 gatgccggcg cacagcaacg gcgccaccgc ggcgagctgc gcctccggat gccggatccg
     1441 aagcacgaag cgctcgtcga cgacgatgcg ctgcgaatag ccgccgagcg tgtggccggg
     1501 cgggtcagcg gtggggctgt tgtaggtggc gacgaagccg ttctcgcaat attgctccag
     1561 cccctcggcg caggacgggc agcgccggca gctgtcgacg aggcagccga ttccgaccag
     1621 gtcgcccgga gcgaagccgc cgacctcgcc gccgacggct ccgacccggc cgacgatctc
     1681 atggcccggc acgcacggat acaccgtgcc gccccattcc gagcgcaccg tgtggaggtc
     1741 ggaatggcag acgccgcaat aggcgatgtc gatctcgacg tcgcgcgggc cggggcggcg
     1801 gcgctcgatg gccatcggct cgagcggccg gtcggcggct cgggcgccat aggctcggac
     1861 ggtcaatgcg cttctcctgg aaatgaaggg ccgcaaggca aaggggacag ccgcgggcgg
     1921 ctgtcccctg ggtcgggcgg ctgtgcccaa ggcgcggagg gacagccgcc gtcggctgtc
     1981 ccgcttagcc taccgacggc ggctgcgctc gtaggtctgg acgcaattgt ccgtgacggt
     2041 gcgtgagcag gccggatggg aggcgtcggc gccggtcgcc gtcgctccgg tggccggatc
     2101 gaccatcggt ccgccgacgc cggtgcccgt cgtgccgttg aagccggcgg ggacgacggc
     2161 cgggtcggag atgaccggga tgccgcgggc atcgcgctcg ggggcggcgt tgctcgggtg
     2221 gacgaccggg ctcatcggcg gggtgacgtt ggccgcgacg gcggtgccgt gggtcatcgt
     2281 cccgtgggtc atcgtcccgt gggtcatcgt gccgtgggtc gtggttccat gggtcgtcgt
     2341 cgtgccgtgg gtcgtcgcgg tgccggtggt cacctcggtt tcggtcgtgc cggcgtcgtc
     2401 gccgacattg tcggtggtgg aggtcacgac g
//
LOCUS       DASVQZ010000303         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_164145, whole genome shotgun sequence.
ACCESSION   DASVQZ010000303 DASVQZ010000000
VERSION     DASVQZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164145"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..421)
                     /gene="hspQ"
                     /locus_tag="VF547_08365"
                     /pseudo
     CDS             complement(131..421)
                     /gene="hspQ"
                     /locus_tag="VF547_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405367.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
     gene            561..650
                     /locus_tag="VF547_08370"
     tRNA            561..650
                     /locus_tag="VF547_08370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:595..597,aa:Ser,seq:cga)
     gene            847..1404
                     /locus_tag="VF547_08375"
     CDS             847..1404
                     /locus_tag="VF547_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX8262873.1"
                     /translation="MQRHGTGEWRVRGALRAATGAVHERLHRAAPFSAIANRQLDVRG
                     YADLVARIAAFHFTVGEDLGTGRLRRGLLVRDLKRLGLSRPGDLPWTSPRTGPERLGW
                     AYVVEGSSIGGKVLYRQLDYLFGRSASGRLFFRGSGSDGPRWQALCRDLEAAGRTDRA
                     VEEIIGGATAAFALFERAVAPAVEA"
     gene            1401..>3036
                     /locus_tag="VF547_08380"
     CDS             1401..>3036
                     /locus_tag="VF547_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8262874.1"
                     /translation="MTSTSGQPPGPDLSACDREPIHIPGAIQPHGVLLALDQDLRVCR
                     AGGDVQGLLGQEKDPLGLRLSDLIPALGVDLPDSEDQFCFVATVNMPAGELDLYAHSC
                     GGELILELEPAPQERRSGAQVLGDLLPIVKQIGAAKDVTEAAAAAASGVRKITGYDRI
                     MIYRFLEDQSGQVIAEARSPDVDSLLHHRYPASDIPQQARALYLRNAVRVIHDVDYSP
                     APIRGGRVPLDLTGALLRSVSPVHIQYLKNMEVGASASFSLVQADSLWGLVACHNRTA
                     RPIAHEEREMCRRVALALDQAVARLEEENSQREALRLTRRREELLPLLVAGESVSAAL
                     RVHLDELRRLVAADGIALLIDDDVVCGGVTPPEPAVRDLGRWLIDQSRDVLATNSLGA
                     LYAPAQAWTATASGLLGAVVARNPLTALLWFRAEEIETVKWAGNPHKPAEPGGSSAQL
                     TPRRSFELWAETVRGRSRPWRANEQETVRRLVSSVAEVGRQKALAELNRQLTSEVAEK
                     DLLMREMHHRVQNSLQLVTSMLQHHEGEGAEEQLAVA"
BASE COUNT          438 a         1028 c         1076 g          494 t
ORIGIN      
        1 cgggcgcttt ggcgcgggac ctaccggcat catgcgcgaa atcaacgcct cgatcggcgt
       61 cgacaagcgc ttgtgggggg aggacatcgc cgcctccaag gcccatgccg cgatgctccg
      121 cgataccggg atcgtcggcg agcagattct gctggctgac ataggcggtg taggaggatt
      181 cgccgttctc tgcgagcagg tggtagaaag gctggtcctt gggcggacgg atgtcctccg
      241 ggatcgcctc gtaccattcg tccgaattgg cgaagaccgg gtcgacgtcg aagatgaccc
      301 cgcgaaagtc gaacagccgg tgccggacca cctcgccgat cgcgaagcgg gcatgggcga
      361 ccggcggcgc cttgggcgcc gggccgttcg ggccggtgaa ggatctgtcg ggccgctcca
      421 tcgccttcct atgtaggccc ttcgccgcgg caggcaagcg gccggccggc tcaggacgcg
      481 cttggcaagc cggaatgctt gggctaagcg ccccgcctgt cgaccggccc ttcgcgcgcg
      541 ccaagggtca atccccatgc ggagaggtgc cggagtggtc gatcggggcg gtctcgaaaa
      601 ccgttgtgcc cttacgggta ccgagggttc gaatccctcc ctctccgcca cttccttcat
      661 ccgccgatgt tcgcccgcgg ccaccgacat gagcgcctgc gtctgaaggc gcctgccgga
      721 ccgcctatgg cgatcgctcg ttcgcgattg gcgcttcgtc agtgtccggt ctggccgcct
      781 gtccgatcgc cgcattcgga gcggatcgat ttgcactccc cggatatcgg gtgcacagcc
      841 aggggcatgc agcggcacgg cacgggggaa tggagggtgc gcggcgcctt gcgcgcggcg
      901 accggcgccg ttcacgagcg gcttcatcgg gcggcgccgt tctccgccat cgccaatcgg
      961 cagctcgacg ttcgcggtta tgcggatctc gtcgcgcgca tcgccgcctt tcacttcacc
     1021 gtcggcgagg acctcggcac gggtcggctg cgccgcgggc tgctcgtccg ggacttgaag
     1081 cgcctcggcc tgtctcggcc gggggacctg ccctggacgt ctcccaggac cgggccggag
     1141 aggctcggct gggcctatgt cgtcgagggc tcgtcgatcg gcggcaaggt cctctaccgc
     1201 cagctcgact atcttttcgg ccgttcggcg agcgggcgcc tcttcttccg cgggtcggga
     1261 tcggacgggc cgcgctggca ggcactctgt cgcgacctgg aggccgcggg ccggacggac
     1321 cgcgccgtcg aggagatcat cggcggcgca accgccgcct tcgctttgtt cgagcgggcg
     1381 gtcgccccgg cggtggaagc atgacgagca cgagcggcca gccgccggga ccggatctca
     1441 gcgcctgcga ccgcgagccg atccacatcc cgggcgcgat ccagccgcac ggcgtccttc
     1501 tggcgctcga ccaggacctg agggtttgcc gcgccggcgg tgatgtccag gggctgctcg
     1561 ggcaggagaa ggatccactc ggactgcgac tctccgatct catcccggcg ctcggcgtcg
     1621 atctcccgga ctcggaggat caattctgtt tcgtcgccac cgtcaacatg cccgccgggg
     1681 agctggatct ctacgcgcat tcctgtggcg gggagctgat cctggagctg gagccggctc
     1741 cccaggagcg ccgctcgggc gcgcaggtgc tcggcgacct gctgcccatc gtcaagcaga
     1801 tcggggccgc gaaagatgtg accgaagcgg cggctgcggc ggcgtcgggg gttcgcaaga
     1861 tcaccggcta cgaccggatc atgatctacc gcttcctcga ggaccagtct gggcaggtca
     1921 tcgccgaagc gcgttccccg gacgtcgact cgttgcttca ccaccgttac ccggcgagcg
     1981 acatacctca gcaggcgcgg gcgttgtatt tgcgcaatgc ggtccgcgtc atccacgatg
     2041 tcgactattc cccggcgccg atccggggag gacgtgtccc gctcgacctg accggcgcgc
     2101 tgctgcgcag cgtctcgccg gtccacatcc agtatctgaa gaacatggag gtgggggcct
     2161 cggcctcctt ctcgctggtg caggccgatt cgctctgggg cctcgtcgcc tgccacaaca
     2221 gaacggccag gccgatcgcc cacgaggagc gcgagatgtg ccgccgagtc gcgctcgccc
     2281 tcgatcaggc ggtcgcccgg ctcgaggagg agaacagcca gcgcgaggcg cttcgactga
     2341 cccgccggcg cgaggagctg ctcccgcttc tcgtggcggg cgagagcgtc tcggcggccc
     2401 tgcgggtcca tttggacgag ctgcgccggc tggtggcggc ggacgggatc gcgctgctga
     2461 tcgacgacga cgtcgtgtgc ggcggagtca ctccccccga gcccgcggtt cgcgatctcg
     2521 ggcgctggct gatcgaccag tcccgcgacg tgctggcgac gaacagtctg ggtgcgctgt
     2581 acgcgccggc acaggcctgg acggccaccg ccagcggact gctcggcgcg gtcgtcgcgc
     2641 gcaatcccct caccgcgctg ctctggtttc gggccgagga gatcgagacg gtgaaatggg
     2701 cgggcaatcc ccacaagccc gcggagcccg gcggctcttc ggcccagctg accccgcgca
     2761 ggtcgttcga gctgtgggcc gaaaccgtaa ggggccgttc gcggccgtgg cgcgcgaacg
     2821 agcaggagac cgtccggcgc ctggtttcga gcgtcgccga ggtcggccgc cagaaggcct
     2881 tggccgagct caatcgccaa ctgacgagcg aagtggcgga gaaggacctg ctgatgcggg
     2941 agatgcacca tcgggtgcag aacagcctgc agctcgtcac gtccatgctc cagcaccacg
     3001 agggggaagg cgcggaagag cagcttgcgg tggctc
//
LOCUS       DASVQZ010000304         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_640361, whole genome shotgun sequence.
ACCESSION   DASVQZ010000304 DASVQZ010000000
VERSION     DASVQZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640361"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VF547_08385"
     CDS             complement(<1..505)
                     /locus_tag="VF547_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEX8262875.1"
                     /translation="MTIRKLLVASLLILTAGCGGAGAEEKGKARPAPVVKVEPATTIR
                     FVETIEAVGTARANEQVTLSAPVTERLVRLGFDDGDFVRRGQVVAVLAQGQESAQLAE
                     AQARQREAQQQLARIQTLKRRGFATQSSLDTQSAAAAAARAQASEARASIGERVIEAP
                     FSGYASLR"
     gene            complement(584..1177)
                     /locus_tag="VF547_08390"
     CDS             complement(584..1177)
                     /locus_tag="VF547_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX8262876.1"
                     /translation="MSDEHEGLELWRSGHRSGVGGIVPKAPAIVLHEPKYPHNVGQVV
                     RLASAFGIAQVWYSGERIREALGARLPREERMRRYEDVALINHPDPLSRLCGALPNAS
                     PVAVELRPGSESLVDFVHPDGDSPPIYVFGPEDGSLSGPVVKRCHRFVVIPSYECLNL
                     ATSVATVLYDRMAKMPASERPSVRGRGPVQAAESRGG"
     gene            complement(1185..1925)
                     /locus_tag="VF547_08395"
     CDS             complement(1185..1925)
                     /locus_tag="VF547_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX8262877.1"
                     /translation="MKLRVDQLLVDRGLAESRAKAQALILAGLVFSGECKIEKAGQAI
                     AGDAQLEVRGKEHPWVSRGGVKLAHALEHFGWDVAGAVALDVGSSTGGFTDVLLQKGA
                     VKVFAVDVGTNQLDWKLRQDPRVVVHEKTNARYLTDAVVTEPVDIVVCDASFISLAKV
                     LDKALGFARPGGRLLALVKPQFEAGRGEIGKGGVVRDAAVHARVCAAAAEWVRGRGWE
                     VEGIVPSPITGPEGNVEFLLAARRPDDR"
     gene            complement(1922..2371)
                     /locus_tag="VF547_08400"
     CDS             complement(1922..2371)
                     /locus_tag="VF547_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX8262878.1"
                     /translation="MADIEELARLTIDCGLRIHKEVGPGLLEAVYEAILASSLLKLGI
                     KADRQVPIGIEYDGLVFAEGFRADLLVDDRLIVEVKSVDRLMPVHGKQVLTYLRLTGR
                     PLGVLLNFGGATLREGIRRIVNNHTDVGSSRLRVNQGPPIQVTGVNG"
     gene            complement(2470..>3848)
                     /gene="dxs"
                     /locus_tag="VF547_08405"
     CDS             complement(2470..>3848)
                     /gene="dxs"
                     /locus_tag="VF547_08405"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX8262879.1"
                     /translation="IAPPAGAMSAYLARLVSGRAYLSIRDAAKQLARKLPRFLHDKAK
                     KTEEYARGFWTGGTLFEELGFYYVGPVDGHNIEGLVKILENVRDADEGPMLVHVVTKK
                     GKGYAPAEAAADKYHGVQKFDVISGEQAKAEPGPPSYTAVFARALTAEAERDGKVVAI
                     TAAMPSGTGLDKFGARFPDRTFDVGIAEQHAVTFAAGLAAQGYRPFCAIYSTFLQRAY
                     DQVVHDVAIQNLPVRFAMDRAGLVGADGATHAGSFDLAYLCTLPNFVVMAAADEVELV
                     HMVHTMALHDDGPIAVRYPRGNGVGKTLPEAPQRLEIGKGRIVREGKKVAILSLGTRL
                     EEALKAAEALEGRGLSTTVADLRFAKPLDEELIRRLLTSHEVAVTVEEAAIGGFGAHV
                     LTLASDLGLIDAGLKLRTLRFPDRFQDQDKPEKQYAEAGVDADGIVEAVLKALRHNSA
                     GVVEGARA"
BASE COUNT          617 a         1330 c         1289 g          612 t
ORIGIN      
        1 tgcgaagcga cgcatagccg gagaagggcg cctcgatgac ccgctcgccg atcgaggccc
       61 gcgcctcgga cgcctgcgcc cgcgccgccg cggccgccgc cgattgcgtg tcgaggctcg
      121 actgggtggc gaagccgcgc cgcttcagcg tctggatccg ggcgagctgc tgctgcgcct
      181 cccgctgccg cgcctgcgcc tccgcgagct gcgcgctctc ctggccctgg gcgagcaccg
      241 cgacgacctg gccgcggcgg acgaaatcgc cgtcgtcgaa gccgagccgg acgagccgct
      301 cggtgacggg cgccgagagg gtcacctgct cgttggcgcg ggcggttccg accgcctcga
      361 tcgtctcgac gaagcggatg gtggtcgcgg gctcgacctt gaccaccggc gcgggccggg
      421 ccttgccctt ctcctcggcg ccggcgcctc cgcaacccgc ggtcaggata agcagcgacg
      481 cgacaaggag ttttcggatc gtcataggca caggccgggc gctagcggct gggggcggag
      541 ctatcaagca agacggtatg cggggcacta cggcaaaaag gccttatccg ccccggcttt
      601 ccgccgcctg aacgggtccg cggccccgga ccgagggccg ctcggacgcc ggcatcttcg
      661 ccatccggtc gtaaaggacg gtggcgaccg acgtcgccag gttcaggcat tcgtaggagg
      721 gaatcacgac gaagcggtga cagcgcttga cgacgggacc ggacagggaa ccgtcctcgg
      781 ggccgaaaac gtagatgggc ggcgagtccc catccggatg gacgaaatcg accaggcttt
      841 ccgagccggg cctgagctcc accgccaccg gggaggcatt cggcaatgcc ccacacagcc
      901 tcgacagcgg gtcgggatga ttgatcaggg cgacatcctc gtagcgcctc atccgctcct
      961 cgcggggaag cctcgcgccg agcgcctctc ggatgcgctc gccgctgtac cagacctgcg
     1021 cgatgccgaa ggcgctggcg aggcggacga cctggccgac attgtgcgga tatttgggct
     1081 cgtgaaggac gatcgccggc gctttcggca cgatcccgcc gacgccgctg cggtggccgg
     1141 agcgccagag ttcaagccct tcatgctcgt cggacatcga gcggttatcg gtcgtcgggg
     1201 cggcgcgccg caagcaggaa ctcgacattg ccctccggcc cggtgatcgg gctcggcacg
     1261 atcccttcga cctcccaacc gcgtcctcga acccactccg ccgccgccgc gcagacccgc
     1321 gcgtggaccg cggcgtcgcg gacgacgcca cccttgccga tctcgccccg cccggcctcg
     1381 aactgcggct tgaccagggc gaggaggcgc ccgccgggcc tggcgaagcc gagcgccttg
     1441 tcgagcacct tggcgagcga gatgaagctg gcgtcgcaca cgacgatgtc gaccggctcg
     1501 gtcaccaccg cgtcggtgag gtagcgggca ttggtcttct catggacgac gacgcgcgga
     1561 tcctgccgaa gcttccagtc gagctggttg gtgccgacgt ccaccgcgaa caccttcacc
     1621 gcccccttct gaagcaagac gtcggtgaag cccccggtcg aagacccgac gtcgagcgcc
     1681 accgcccccg ccacgtccca cccgaaatgc tcgagcgcat gcgccagctt gaccccgccc
     1741 cgcgaaaccc acggatgctc cttgccccgc acctcgagct gagcatcgcc ggcgatggcc
     1801 tgaccggctt tctcgatctt gcactcgccg ctgaacacca acccggccaa gatcaaggcc
     1861 tgcgccttcg cccggctctc ggcgaggccg cggtcgacca gcaactggtc gactcgcagc
     1921 ttcatccgtt gacgcccgtg acctggatcg gcggcccttg gttcacgcga agacgcgaag
     1981 atccaacgtc cgtgtggtta ttgacgatac gcctgatgcc ctcccggagc gtggcgccgc
     2041 caaagttcag aagaacgcca aggggtcttc cggtcagccg aagataggtg aggacctgct
     2101 tgccgtggac cggcatcagt cgatccaccg atttcacctc gacgatcagc cggtcgtcaa
     2161 ccagcaaatc cgcgcggaat ccttccgcaa aaaccaagcc gtcatattct atgccgatcg
     2221 gcacttgccg atcggccttt atccccagct tcaggagact ggatgcgaga atggcctcat
     2281 agacggcttc gaggagaccg ggccccacct ccttgtgaat ccggagaccg cagtcgatcg
     2341 tcagccgggc aagctcctcg atatccgcca tcccagatcc ttcgcgttct tcgcgcacct
     2401 tcgcgtcttc gcgtgaaata taacgatgtt cagggaatat cgcgacgtta ttctacaaaa
     2461 gcaatgaagt caggcccgcg cgccctcgac gaccccggcg ctattgtgcc ggagcgcctt
     2521 cagcaccgcc tcgacgatgc cgtcggcgtc gactccggcc tcggcgtatt gcttctcggg
     2581 cttgtcctgg tcctggaagc ggtcggggaa gcgcagggtg cgcagcttca ggccggcgtc
     2641 gatcaggccg aggtcgctcg ccagcgtgag gacgtgggcg ccgaagccgc cgatcgccgc
     2701 ctcctcgacg gtgaccgcga cttcgtggct ggtcagcagc cggcggatca gctcttcgtc
     2761 gagcggcttg gcgaagcgca ggtcggcgac ggtggtcgag aggccgcggc cctcgagcgc
     2821 ctcggcggcc ttgagcgcct cctccaggcg ggtgccgagc gagaggatgg cgaccttctt
     2881 gccctcgcgg acgatccggc ccttgccgat ctcgaggcgc tgcggagcct cgggaagcgt
     2941 cttcccgacg ccgttgccgc gcgggtagcg gaccgcgatc gggccgtcgt cgtggagcgc
     3001 catggtgtgg accatgtgga ccagctcgac ctcgtccgcc gccgccatca cgacgaagtt
     3061 tggcaaagtg caaagatagg caaggtcgaa gctgccggca tgggtggcgc cgtcggcgcc
     3121 gaccaggccg gcccggtcca tggcgaagcg caccggcagg ttctggatgg cgacgtcgtg
     3181 gacgacctgg tcgtaggcgc gctgcaggaa ggtcgaataa atggcgcaga acgggcggta
     3241 gccctgggcg gcgaggccgg cggcgaaggt gacggcgtgc tgctcggcga tgccgacgtc
     3301 gaacgtccgg tcgggaaagc gggcgccgaa cttgtcgagg ccggtgcccg acggcatcgc
     3361 cgcggtgatc gccaccacct tgccgtcgcg ctcggcttcc gcggtcagag ccctggcgaa
     3421 gaccgcggtg tagctcggcg ggccgggctc ggccttggcc tgctcgccgg agatcacgtc
     3481 gaacttctgg actccgtgat atttgtcggc cgccgcctcg gccggggcat agcccttgcc
     3541 tttcttggtg acgacatgga ccagcatcgg gccttcgtcg gcgtcgcgga cattctccag
     3601 gatcttgacc agcccctcga tattgtggcc gtcgaccgga ccgacataat agaagccgag
     3661 ctcctcgaag agcgtgccgc cggtccagaa gccgcgcgca tattcttccg ttttcttcgc
     3721 cttgtcgtgc aggaagcgcg gcagtttgcg cgccagctgc ttggccgcgt cacggatgga
     3781 aaggtaggcg cgcccggaaa cgagccgcgc cagataggcg ctcatcgccc cggccggcgg
     3841 ggcgatcg
//
LOCUS       DASVQZ010000305         6300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_739432, whole genome shotgun sequence.
ACCESSION   DASVQZ010000305 DASVQZ010000000
VERSION     DASVQZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739432"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VF547_08410"
     CDS             <1..129
                     /locus_tag="VF547_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8262880.1"
                     /translation="AFDDTIEHEAWNDSGEPRTILILDAWNPLLTEAERAAVRIIG"
     gene            complement(230..1003)
                     /locus_tag="VF547_08415"
     CDS             complement(230..1003)
                     /locus_tag="VF547_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8262881.1"
                     /translation="MNGASGSRREARRDRSQRLAVDLLLLAAFGLAMATLGPYRTLDV
                     PDGLRTAYWLLAVAGAGFAGIAVDLALGPRIRGFWARIGVVSLVTTLPVTPFIYALNA
                     ALLDLPRRPWLLPQLAWQVLVVLLLIMALRALLWRRIVETRTVVVPPLPEAERAFRMR
                     LSAKRRGARLIALEAEDHYVRVHTDAGSELVAMRFSEAVEQLALAHGYRLHRSWWAAA
                     DAVEAVRWKRGTGEARLAGGLTAPVSRGCAAALKEAGWR"
     gene            complement(1208..1906)
                     /locus_tag="VF547_08420"
     CDS             complement(1208..1906)
                     /locus_tag="VF547_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8262882.1"
                     /translation="MSETDRDGRRPGWGALLMEGPAAVRLLAAQLRPARVREDVGRGR
                     PVMVLPAFLANDLPTIQLRRTLRACGFRSYGWRQGLNVGARRGKFERLLRRIDMIAAE
                     NRSPVALVGWSLGGLYARELAKRRPQAVSMVVTLGTPFSQGLRANNAWKLYETINDHD
                     VDHPPVPIAPEAKPPVPTVAMWSPLDGIVAPASAAGRDEESDRRVKLSCRHNELVSDP
                     AALQAVVAALAAAA"
     gene            complement(1988..2467)
                     /locus_tag="VF547_08425"
     CDS             complement(1988..2467)
                     /locus_tag="VF547_08425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8262883.1"
                     /translation="MPPARHFRWACEADFGLLADIMFDAVRNGESPYSEPQRAAWVPE
                     RRSGEDWAARLRRQDIIVAEQDGEAVGFASLAEGGYVDFAYIRPGAQHSGLFRQLLSR
                     LVERAVARREPRLWTHASLAAEPAFAKLGFTIRKRERVRIGDQELDRCEMEKPLTTD"
     gene            2645..3310
                     /locus_tag="VF547_08430"
     CDS             2645..3310
                     /locus_tag="VF547_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8262884.1"
                     /translation="MRRITGAAFVSLDGVMQAPGGPEEDPTGGFELGGWLAGFFDEEI
                     GERIDALFEPPFDLLLGRRTYDIFAAYWPFNADEPGGIGQKFDAAAKYVVTRGGQALD
                     WRNSHRLESLEAVAEMKRGEGPDLVIQGSSTLYPQLLGAGLIDRLVLMIFPVVLGSGK
                     RLFGEGTSPGAMRLVEHRVTARGNIVAAYEPHGPVQPGSFATGEPSAAETERQAKIRE
                     GCW"
     gene            complement(3445..3978)
                     /locus_tag="VF547_08435"
     CDS             complement(3445..3978)
                     /locus_tag="VF547_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262885.1"
                     /translation="MRGWVIAVAAAALAAPAALPAQKAKPQARPEAFEALVRCRALAD
                     DAARLRCFDSAAATLQQQAENRDLVVVDRKQIQETKRGLFGLDIPNLNPFGGGGDDGV
                     EEIKSIESTIRSAMQDGDGRWIVTLEDGSTWAQTDSYPFAVNPKRGHKVKVVKASMGS
                     YMMRVNNQPGVRAKRRI"
     gene            4097..5185
                     /locus_tag="VF547_08440"
     CDS             4097..5185
                     /locus_tag="VF547_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262886.1"
                     /translation="MSPAAGLSAGALAATPLWFPQFLDPATDRVLLVERTEADYRDSA
                     FLDERSLKPDRPRHAVEWWHLAAAFPPAARRDVQYIFHIGHVGSTLVSRLLGELPQLL
                     SLREPLVLRTFAEMLGERGRAEALWDPVSIPGRLDVLTALLSRTFRAGQRALVKATSF
                     VSEIAADLVPTGSRALLLYARPERYFETVLAGPNSRQELAMTAQGRLKRLHRSVGGDR
                     WRLWEMGEGERLAMAWAAEMASLVRAAEALPAGSATWLDFDAFLDAPAPSLAALAAFF
                     GVPLDPAGAEHLAADPLMRRYSKAAEYEYSRELREEVLAEARREQAPAIAAALRWLDS
                     AGRDCAQVGRALDRAARGSTDAGPAANR"
     gene            5190..5843
                     /locus_tag="VF547_08445"
     CDS             5190..5843
                     /locus_tag="VF547_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX8262887.1"
                     /translation="MFKEIPDLLTADEVQALRRIAAASKWIDGRQSNPHSSVKNNLQL
                     YEKTAADLLARALYRSEDFTNFTFPVVLAPPMLSRYEPGMHYGGHADAAYLPLGEKPL
                     RCDLSCTIFLSDPDSFEGGALKIRLGSRELRFRGAAGSAVVYPSTTFHEVEPLSRGER
                     LAGITFIQSRIADAFLRELLYELNEVAALEGLNMDPENYARLQLVQRNLLQRWGDMP"
     gene            6040..>6300
                     /locus_tag="VF547_08450"
     CDS             6040..>6300
                     /locus_tag="VF547_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262888.1"
                     /translation="MNKNRILNNLMATTFICGAIGFAAPAYAQDTEEPAAPQAGPVEN
                     ADPAVSADAAPEGQGQDTEGDIIVTGTRIPQPNLNSASPITVL"
BASE COUNT          909 a         2338 c         2150 g          903 t
ORIGIN      
        1 gcgttcgacg acacgatcga gcacgaggcc tggaacgaca gcggcgagcc gcgcaccatc
       61 ctcatcctcg acgcctggaa cccgctgctc accgaggccg agcgcgccgc cgtccgaatc
      121 atcggctgac ccgcgaacgg ccggaacgct ccgcgatcgg cgccagcgtg acctcgccga
      181 gaccctccgc ggctcaaccc ttgtcagaga gtcccctcac cggccgcttt cagcgccagc
      241 cggcctcctt cagggccgcc gcgcagcccc ggctcaccgg cgccgtcagc ccgcccgcca
      301 gtcgcgcctc gccggtgccg cgcttccagc gcaccgcttc gacggcgtcg gccgccgccc
      361 accaggaccg atgcagccga tagccatgcg ccagggcgag ctgttcgacg gcttcggaga
      421 agcgcatcgc gaccagctcc gagccggcgt cggtatggac ccgcacataa tggtcctcgg
      481 cctcgagcgc gatcagcctc gcgccgcgcc gcttcgccga aagccgcatc cggaaggcgc
      541 gctcggcctc cggcagcggc ggcacgacga ccgtccgggt ctcgacgatc cgccgccaca
      601 gcagcgcgcg cagggccatg atcagcagca gcacgacgag cacctgccag gcgagctggg
      661 gaagcagcca cggccgcctc ggaaggtcga gcagggcggc gttcagcgca tagatgaacg
      721 gggtgaccgg gagggtcgtc accagcgaga cgactccgat ccgggcccag aagccccgga
      781 tgcgcggtcc cagcgccagg tcgacggcga ttccggcgaa gccggcgccg gccacggcca
      841 gcagccagta ggcggtcctc aggccgtccg ggacgtcgag cgtgcggtac gggccgagcg
      901 tcgccatcgc cagcccgaag gcggcgagca gcagcaggtc gaccgcgagg cgctggctcc
      961 gatcccgccg cgcctcccgt cgcgaacccg atgcaccgtt cacgcgaaac cgcctttcct
     1021 accccgaagg ccgggccccc gtctgcgcgc gttcaagcga tgcggagctt atcacgatcc
     1081 gaggcccgat ccacccggcc gcccggcgcc cggtcgtcgt cccgctccgc ggccgggatc
     1141 gcgatcgtct ccgggctccg acggccttca gggcgaaggt cgcaccgcct cgaagccgct
     1201 ttgcctctca ggcggcggcg gcgagcgccg cgacgacggc ctggagcgcg gcgggatcgc
     1261 tgaccaactc gttgtggcgg caggagagct tcacgcgccg gtccgactcc tcgtcccggc
     1321 cggcggcgct ggccggagcg acgatgccgt cgagcggcga ccacatcgca acggtcggca
     1381 ctggcggctt cgcctcgggc gcgatcggca ccggcggatg atcgacgtca tggtcgttga
     1441 tcgtctcgta gagcttccac gcattgttcg cgcgcagccc ctgcgagaag ggcgtgccga
     1501 gggtgacgac catcgacacc gcctgcggcc gccgcttcgc cagctctcgc gcatagagcc
     1561 cgcccaggct ccacccgacc agcgcgaccg ggctccggtt ttccgccgcg atcatgtcga
     1621 tccgcctcag cagccgctcg aacttgccgc gccgggcgcc gacattgagc ccctgcctcc
     1681 acccatagct cctgaagccg caggcgcgca gggtccgccg aagctggatc gtcggcaggt
     1741 cgttggcgag gaaggcgggg agcaccatca ccggccggcc gcggccgaca tcctcccgga
     1801 cccgcgccgg gcggagctgc gccgcgagca gccgcaccgc cgccggcccc tccatcagca
     1861 gcgcgcccca gcccggccgc cgcccgtccc gatccgtctc cgacaccgct ttccttccgt
     1921 tggtggggcc aaagcagtcc agcccccgat cggttcccgc ggaaaagcgg acggccggcg
     1981 cctcgcttca gtcagtcgtc aggggcttct ccatctcgca ccggtcgagt tcctggtcgc
     2041 cgatgcggac gcgctcccgc ttcctgatcg tgaacccgag cttggcgaag gccggctcgg
     2101 ccgccaggct cgcatgcgtc cacagcctcg gctcgcgccg ggcgacggcc cgttcgacga
     2161 ggcgcgagag cagctggcgg aagaggccgc tgtgctgcgc tccgggccgg atgtaggcga
     2221 aatcgacata gccgccctcg gccagactgg cgaagccgac cgcctcgccg tcctgctcgg
     2281 ccacgatgat gtcctgcctg cgcaggcggg cggcccaatc ctccccggag cggcgctctg
     2341 gaacccaggc cgcccgctgc ggctccgaat aagggctttc gccgttcctc acggcgtcga
     2401 acatgatgtc ggcgagcagg ccgaagtcgg cctcgcaggc ccagcggaaa tgacgggcag
     2461 gaggcattgg ctcacgaaat ccggcggcgt tccatgagct tgccggaacg cgcggacgtc
     2521 cgcaatggcc gcaagcagtg gtgccgaaat ccgccggtcg ggcggcaccc cggaggcgcg
     2581 tccgctggcg agcttgacct ccgcgggccg ccgggctttc ctcggcgacg gcaggaggat
     2641 gatgatgcgc aggataaccg gggccgcctt cgtttcgctg gacggcgtga tgcaggcgcc
     2701 gggcgggccg gaggaggatc cgaccggcgg cttcgagctg ggcgggtggc tggccggctt
     2761 cttcgacgag gagatcgggg agcggatcga cgctctgttc gagccgcctt tcgacctcct
     2821 cctcgggcgg cggacctacg acatcttcgc cgcctactgg ccgttcaatg ccgacgagcc
     2881 cggcgggatc gggcagaagt tcgacgccgc cgccaaatat gtcgtgaccc gcggcggcca
     2941 ggccctcgac tggcggaaca gccaccgcct ggagagcctc gaggccgtcg ccgagatgaa
     3001 gcggggggag gggccggacc tggtcatcca gggcagctcc accctctatc cgcaattgct
     3061 cggagcgggg ctgatcgacc ggctggtgct gatgatcttc cccgtcgtgc tgggatcggg
     3121 caagcgcctg ttcggcgagg gcacctcgcc gggcgcgatg cgcctcgtcg agcaccgggt
     3181 gaccgcgcgc ggcaatatcg tcgccgccta cgagccgcac ggcccggtcc agcccggaag
     3241 cttcgcgacc ggcgagccca gcgccgcgga gaccgagcgc caggcgaaga tccgcgaggg
     3301 ctgctggtag gcgccgccgg gcaggcgcgc gtcccgagga tcgttgcgcc gaaggcaggc
     3361 gcacgcccga ggattttgtt ttcgcggagc ttcggaactt gccgcgcccc gttgccgctt
     3421 cgcggccggg tgtcccgggc cgtttcagat ccgccgtttc gcgcggaccc ccggctggtt
     3481 gttgacccgc atcatgtagc tgcccatcga ggccttgacg accttgacct tgtggccgcg
     3541 cttcgggttg accgcgaacg gatagctgtc ggtctgggcc caggtgcttc cgtcctcgag
     3601 cgtgacgatc cagcggccgt cgccgtcctg catggcggag cggatcgtgc tctcgatcga
     3661 cttgatctcc tcgaccccgt cgtcgccgcc gccgccgaac gggttcagat tggggatgtc
     3721 gaggccgaac aggccgcgct tggtctcctg gatctgcttc ctgtcgacga cgacgaggtc
     3781 gcgattctcg gcctgctgct ggagcgtcgc ggcggctgaa tcgaagcagc gcaggcgggc
     3841 ggcgtcgtcg gccaaagcgc ggcagcggac cagggcctcg aacgcctcgg gccgcgcctg
     3901 cggcttggcc ttctgggccg gaagcgccgc cggagccgcc agtgccgccg ccgcgaccgc
     3961 aatcacccac ccgcgcatgt ccagtccctt ccgctcgtct cgccgcgata ttggggcaag
     4021 ggcccggccc gcgcaagccg ccagcggagt cccgcccgct tgcctcgcgc cccccgcccc
     4081 ggctagcggg accggaatga gccctgcagc cggtctttcc gcgggagcgc tggcggcgac
     4141 gccgctctgg ttcccccaat tcctcgaccc ggcgacggac cgggtcctcc tcgtcgagag
     4201 gacggaggcg gactatcgcg attcggcctt cctcgacgag cgctcgctga agcccgaccg
     4261 gccccgccac gcggtcgaat ggtggcacct cgccgccgcc ttcccgccgg cggcgcggcg
     4321 cgacgtccag tacatcttcc atatcggcca tgtcggctcg accctcgtct cgcgcctgct
     4381 cggcgagctg ccgcagctcc tgtcgctgcg cgagccgctg gtccttcgca ccttcgccga
     4441 gatgctcggc gagcgcggtc gggccgaggc cctgtgggac ccggtttcta tcccgggccg
     4501 actcgacgtc ctgaccgcgc tgctgtcgcg caccttccgg gccgggcagc gggcgctggt
     4561 caaggcgacc agcttcgtca gcgagatcgc cgccgatctc gtgccgaccg gctcgcgggc
     4621 cctgctgctc tacgcccggc ccgagcgcta tttcgagacc gtcctcgccg gccccaattc
     4681 ccgccaggag ctggcgatga ccgctcaggg ccggctgaag cggctccacc gctcggtggg
     4741 cggggaccgc tggcggctgt gggagatggg ggagggcgag cgcctggcga tggcctgggc
     4801 ggccgagatg gcgagcctgg tccgcgccgc cgaggcgctt ccggcgggga gcgcgacgtg
     4861 gctcgacttc gacgccttcc tcgacgcccc ggcgccgtcg ctggcggcgc tcgcagcctt
     4921 cttcggggtg ccgctcgacc cggcgggagc cgagcatctc gccgccgacc cgctgatgcg
     4981 ccgctactcc aaggccgccg aatatgaata tagccgcgag ctccgcgagg aggtgctggc
     5041 cgaggctcgg cgcgagcagg cgccggcgat cgccgcggcg cttcggtggc tggactcggc
     5101 gggccgcgac tgcgcccagg tcgggcgggc gctggaccgc gcggcgcgcg gctcgacgga
     5161 cgccggccca gccgctaatc ggtgagccga tgttcaagga gatccccgac cttctgaccg
     5221 ccgacgaggt ccaggcgctg cggcgcatcg cagcggcctc gaaatggatc gacgggcgcc
     5281 agagcaatcc ccattcgtcg gtgaagaaca acctccagct ctacgagaag acggcggcgg
     5341 acctgctggc ccgggcgctc tatcggagcg aggacttcac caatttcacc ttccccgtcg
     5401 tgctcgcgcc gccgatgctg agccgctacg agccggggat gcattacggc ggccatgccg
     5461 acgccgccta cctgccgctg ggcgagaagc cgctgcgctg cgacctcagc tgcaccatct
     5521 tcctctcgga tcccgacagc ttcgaagggg gcgctctgaa gatccgcctc ggctcgcgcg
     5581 agctgcgctt ccggggcgcc gcgggatcgg cggtggtcta tccctcgacc accttccacg
     5641 aggtcgagcc gctctcccgc ggcgagcggc tggccggcat caccttcatc cagagccgca
     5701 tcgccgacgc cttcctgcgc gagctcctct acgagctcaa cgaggtcgcc gcgctcgaag
     5761 gcctgaacat ggacccggaa aactatgccc gcctccagct cgtccagcgc aacctgctgc
     5821 aacgctgggg ggacatgccg tgaaccggcg gccggtcggg gagccggctc acccgtgacc
     5881 caaaagccac accggaggct gtttcgctag ggcgaagccg ccagcgaatt gcttcccttt
     5941 gatgtcttcc cttagagaag acttgggtcg ccgacacttg gacggcgcta cagggatgga
     6001 agaaatctgc ggccaaaagg ccgttacaag gggacggaca tgaataagaa ccgcattctc
     6061 aataacttga tggcgacgac gtttatctgc ggcgccatcg gtttcgccgc gccggcttat
     6121 gcgcaggaca ccgaggagcc cgccgccccg caggccggcc cggtcgaaaa tgccgatccc
     6181 gccgtctccg cggacgccgc tcccgagggc cagggccagg acaccgaagg cgacatcatc
     6241 gtcaccggca cccgcatccc gcagccgaac ctgaattcgg ccagccccat caccgtcctc
//
LOCUS       DASVQZ010000306         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_641001, whole genome shotgun sequence.
ACCESSION   DASVQZ010000306 DASVQZ010000000
VERSION     DASVQZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641001"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VF547_08455"
     CDS             <1..1296
                     /locus_tag="VF547_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEX8262889.1"
                     /translation="LGILSVREEIALEPAFWAQFPGNFRYIARRGLISTANFAGLASG
                     HNFPLGRASGNHRGEAVTLLETTAAGPYYFNFHRGDLGNFTVIGPSGSGKTVVLNFLL
                     AQARRFRPRIIFFDKDRGAELFIRAIGGRYDLLRPGTPSGLNPLQLPDNPANRQFLID
                     WVALLAGGADIEEIARIKDAIDANFEQPADHRRLRHLAELFRGGHRPSAADLWSRLNP
                     WWGDGERAWLFDNPLDKTDLSAESVGFDMTQILDDPAMRTPAMMYLFHRVEERLDGSA
                     AIIVVDEGWKALDDDVFVRRIKDWEKTIRKRNGIVGFATQSAQDALESRIASAIIEQA
                     ATQIFMANPKARADDYIEGFGLTPHEYELIRTLPDNAHCFLIKHGAESVVARLNLTGE
                     RDLLTILSGRERTVRLLDEIRAERGDDPADWIPRLLEVA"
     gene            1296..>2225
                     /locus_tag="VF547_08460"
     CDS             1296..>2225
                     /locus_tag="VF547_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEX8262890.1"
                     /translation="MAACPAIPPVEGFLRGVMDFVDCQAQSIGAEGYRALASPGSTLS
                     LVLLGFLTLFVALFGYRMLLGHVPGVRDAVLALVKIGLVLALATGWGAYRTLIYDVAF
                     RGPAEIAAEIGRPAALPGAGGGLVVRLDYADRAMVELARLGPGDDLSAARTTPAAEPG
                     QPPLPTAAARQARGYDMLALDGARVLFLAGAIGAMAAVRLAAGLMLALGPFFIAFLLF
                     EGTRGLFEGWLRVLGGAALGALGAPIMLGVELAMLEPWLTHLLALRNAGEPIAGVPVE
                     LFVVTLVFALALLGTLIAAARVAYGFRLPPVWRE"
BASE COUNT          304 a          802 c          744 g          375 t
ORIGIN      
        1 ctcggcatcc tgtcggtgcg tgaggagatc gcgctggagc cggccttttg ggcgcagttt
       61 ccaggcaact tcagatatat tgcgcgccga ggtctgatct cgacagcgaa cttcgccgga
      121 cttgccagcg gccacaactt cccgctcggc cgcgccagcg gcaaccatag gggcgaggcg
      181 gtgaccctgc tggagacgac ggcggcgggc ccttattatt tcaacttcca ccgcggcgat
      241 ctcggcaact tcaccgtcat cggcccctcg ggttcgggca agacggtggt gctgaacttc
      301 ctgctcgccc aggcccggcg gttccggccg cggatcatct tcttcgacaa ggatcgcggc
      361 gccgagctgt tcatccgcgc gatcggcggc cgctacgatc tgctgcggcc gggcacgcct
      421 tccgggctga acccgctcca gctcccggac aatccggcca accggcaatt cctgatcgac
      481 tgggtggcgc ttctggccgg cggcgcggac atcgaggaga ttgcgcggat caaggacgcg
      541 atcgacgcca atttcgagca gcccgccgac caccggcgcc tgcgccattt ggccgagctc
      601 ttccgcggcg gccaccggcc gagcgcggcc gatctctggt cgcgcctgaa cccctggtgg
      661 ggcgatggcg agcgcgcctg gctgttcgac aacccgctcg acaagaccga cctcagcgcc
      721 gaatcggtcg gcttcgacat gacccagatc ctcgacgatc cggcgatgcg cacccccgcg
      781 atgatgtatc tgttccaccg cgtcgaggag cggctcgacg gcagcgctgc gatcatcgtc
      841 gtcgacgaag gctggaaggc cctcgacgac gacgtgttcg tccgccggat caaggattgg
      901 gagaagacga tccggaagcg caacggcatc gtcggcttcg ccacccagag cgcccaggac
      961 gcgctcgaga gccgcatcgc cagcgccatc atcgagcagg ccgcgaccca gatcttcatg
     1021 gccaacccca aggcccgggc cgacgattat atcgagggct tcggcctgac cccgcacgaa
     1081 tacgagctga tccgaaccct ccccgacaac gcccattgct tcctgatcaa gcacggcgcg
     1141 gaaagcgtcg tggcgcgcct caacctcacc ggggagcgcg acctgctcac catcctttcc
     1201 ggccgcgagc gcaccgtgcg gctgctcgac gagattcgcg ccgagcgcgg cgacgatccg
     1261 gcggactgga tcccgcgcct gctcgaggtg gcgtgatggc ggcctgcccc gccatccccc
     1321 ccgtcgaggg cttcctgcgc ggcgtgatgg atttcgtcga ctgccaggcg cagtcgatcg
     1381 gcgccgaagg ctatcgagcg ctcgcctcgc ccggctcgac gctgtcgctg gtcctgctcg
     1441 gcttcctgac cctgttcgtc gcgctgttcg ggtaccggat gctgctcggc cacgttcccg
     1501 gggtccgcga cgcggtgctc gccctggtca agatcggcct ggtcctcgct ctggccaccg
     1561 gctggggcgc ctatcggacc ttgatctacg acgtcgcctt ccgcggcccg gccgagatcg
     1621 cggccgagat cggccggccg gccgctttgc cgggcgcagg cggcggcctc gtcgtgcggc
     1681 tggattatgc ggaccgggcg atggtcgagc tggccaggct cggccccggc gacgatctct
     1741 ccgccgcccg gacgactccc gccgcggagc cggggcagcc gccgctcccc accgccgctg
     1801 cgcgccaggc gcggggctac gacatgctgg cgctcgatgg ggcccgggtc ctgttcctcg
     1861 ccggagcgat cggagcgatg gccgcggtcc ggctggccgc cgggctgatg ctcgcgctcg
     1921 gccccttctt catcgccttc ctgctgttcg aaggcacacg cggattgttc gaaggatggc
     1981 tgagggtgct cggcggggcc gcgctcggag cgctcggagc gccgatcatg ctgggggtcg
     2041 agctggccat gctcgagccc tggctgaccc atctcctcgc gctgcgcaac gcgggcgagc
     2101 cgattgccgg agtccccgtc gagctgttcg tcgtgaccct ggtcttcgct ttggccctgc
     2161 tcggcacgct gatcgccgcg gccagggtcg cctacggctt ccgcttgcct ccggtgtggc
     2221 gtgaa
//
LOCUS       DASVQZ010000307         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_64006, whole genome shotgun sequence.
ACCESSION   DASVQZ010000307 DASVQZ010000000
VERSION     DASVQZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64006"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /gene="smpB"
                     /locus_tag="VF547_08465"
     CDS             <1..442
                     /gene="smpB"
                     /locus_tag="VF547_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX8262891.1"
                     /translation="VAENRRARFDYAIEDKYEAGIALTGTEVKSLRFGEGSIAESYAE
                     VRDDEAWLINANIPEFSHGNRHNHEPKRPRKLLLHEREIAKLHGAVARQGMTLVPLSI
                     YFNSRGRAKVELALARGRKAPDKREYQKEKDWKREQGRLLRDRG"
     gene            480..989
                     /locus_tag="VF547_08470"
     CDS             480..989
                     /locus_tag="VF547_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEX8262892.1"
                     /translation="MPKREELAESRWLKPFGKRVLHSEFWRFTRRSVPRGVAIGLLVG
                     IFVMIPGLQIVGSALLCVPCRGNIPIAALMTFLSNPFTTPFILLASLEIGSKLGFRTD
                     IASFYALRDSGAGLGAWVKWLFSDAAPALVSGLFIIGFGAALIGYLLSILVWRWWTAR
                     KWRRRAGAA"
     gene            1121..>1871
                     /locus_tag="VF547_08475"
     CDS             1121..>1871
                     /locus_tag="VF547_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8262893.1"
                     /translation="MSTVLSSAPSARPRLGLALPAMLAAAAASAALLLWALDNAVFAA
                     AFFAGALAVGLPLLLAGRGPVEAAAPVEPGTDLALVRSALEASPDALAITDSSAALLA
                     ANGRYESWFGIRAPLELPFGEALGPLLAAARRDGRTEASGLAAGELSLAAEVRAAGQG
                     EGTLLWRFARSGEKDPTAEARELLAGEIGRRLGASGLMAVLTAADGRVLEANGAFHAR
                     AGGEAEVAPGTPLVDLLAMGEQHQFHFAREGK"
BASE COUNT          245 a          689 c          663 g          274 t
ORIGIN      
        1 cgtcgccgag aaccggcgcg cgcgcttcga ttatgcgatc gaggacaaat atgaggccgg
       61 gatcgcgctg accggaaccg aggtgaagtc gcttcgcttc ggcgagggct cgatcgccga
      121 gagctatgcc gaggtgaggg acgacgaggc ctggctgatc aacgccaaca tccccgagtt
      181 cagccacggc aaccgccaca atcacgagcc caagcgaccg cgcaagctgc tcctccacga
      241 gcgcgagatc gccaagctcc acggggccgt cgcccgccag ggcatgaccc tcgtgccgct
      301 gtccatctac ttcaacagcc gcggccgggc gaaggtcgag ctggcgctgg ccagggggcg
      361 caaggcgccc gacaagcgcg aatatcagaa ggagaaggac tggaagcggg agcagggccg
      421 gctgttgcgc gaccgtggct gaccgggagg gccgcctttt ccgctgggcc aggcgcaaca
      481 tgcccaagcg cgaggagctc gccgagagcc gctggctgaa gccgttcggt aagcgagtcc
      541 tgcattcgga attctggcgc ttcacccgcc gctcggtccc gcgcggagtc gcgatcggcc
      601 tcctcgtcgg catcttcgtg atgatccccg gcctgcagat cgtcggctcg gcgctgctgt
      661 gcgtgccctg ccgcggcaac atcccgatcg ccgccttgat gaccttcctc tccaacccct
      721 tcaccacccc gttcatcctg ctcgcctcgc tcgagatcgg cagcaagctg ggcttcagga
      781 ccgacatcgc cagcttctac gctctgcgcg acagcggcgc cgggctcggc gcctgggtga
      841 aatggctctt ctccgatgcc gcgccggcgc tcgtctcggg cctgttcatc atcggcttcg
      901 gcgcggctct gatcggctat ctgctctcga tcctggtgtg gcggtggtgg acggcgcgca
      961 aatggcggcg gcgcgcgggc gcagcctagg cgccgcgtgc gacgacttcc aacaggtcgc
     1021 ccggccgaag gctccgttgg ccgcccgggc cgcgggtccg caggcggcgg ccgccggccg
     1081 cccttgacct gagggagaga aagaaaggat gggtcggccg atgtcgaccg tcctttcctc
     1141 cgccccgtcc gcccggccgc gcctcggcct cgccctgccg gcgatgctcg ccgccgccgc
     1201 ggcctcggcg gccctgctgc tttgggccct ggacaacgcc gtcttcgccg cggccttctt
     1261 cgccggcgcg ctggccgtgg ggctgccgtt gctgctcgcc ggacgcggcc cggtcgaggc
     1321 cgccgccccc gtcgagccgg ggacggatct cgccctggtc cggtccgcgc tcgaagcgtc
     1381 gcccgatgct ctggcgatca ccgactcgag cgccgccttg ctcgccgcca acggccgcta
     1441 cgaaagctgg ttcggaatcc gcgcgccgct cgagcttccg ttcggcgagg cgctcggccc
     1501 gcttctcgcc gcggcgcgcc gcgacggtcg gaccgaggcg tccggcctcg ccgccggcga
     1561 gctgagcctc gccgccgagg tccgcgccgc ggggcagggg gaaggcaccc tgctctggcg
     1621 cttcgcccgg tccggcgaga aggatcccac cgcggaggcc cgcgagctgc tggcgggcga
     1681 gatcggccgc cgcctcgggg cgagcgggct gatggcggtc ctcaccgcgg ccgacggccg
     1741 ggtcctggaa gcgaacgggg cgttccacgc ccgggccggc ggcgaggccg aggtcgcgcc
     1801 gggcacgccg ctggtcgacc tgctggcgat gggcgagcag caccagttcc acttcgcccg
     1861 cgagggcaag g
//
LOCUS       DASVQZ010000308         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_64090, whole genome shotgun sequence.
ACCESSION   DASVQZ010000308 DASVQZ010000000
VERSION     DASVQZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64090"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1094)
                     /locus_tag="VF547_08480"
     CDS             complement(51..1094)
                     /locus_tag="VF547_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8262894.1"
                     /translation="MTDTEANSVGRDRLRRLMKRGTDFLGCEVAILGGAMSWISERNL
                     VSALSNAGAFGVIACGAMTPDLLDTEIGETRLRTERPFGVNLITMHPQLSELIDVCAR
                     HRVSHVVLAGGLPPPGSIDRIKGNGAKLICFAPALALAKKLVRSGVDALVIEGMEAGG
                     HIGPVSTSVLAQEILPPLAAEIPIFVAGGIGHGGLIAGYLEMGAVGVQLGTRFVCAHE
                     CIAHPNFKKAFIRASARDAVTSVQIDPRLPVIPVRALKNREMESFAAKQREVADLLDR
                     GEVEMAEAQLQIEHYWAGALRRAVIEGDVETGSVMAGQSVGMVTKEEPVADILRSLVD
                     QAAAALERRAVHA"
     gene            complement(1189..2430)
                     /locus_tag="VF547_08485"
     CDS             complement(1189..2430)
                     /locus_tag="VF547_08485"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX8262895.1"
                     /translation="MARIVMKFGGTSMAGIERIRHVAQRVKDEAEAGNQVAVVVSAMA
                     GETDRLIQFCREASSLYDPREYDVVVASGEQVTSGLLAITLQGMGLNARSYMGWQLPI
                     RASGHASALIDDIDATVLERVFGEGGIAVIPGFQGVAADGSLATLGRGGSDTSAVALA
                     AAVNADRCDIYTDVDGVYTTDPRIVPRARKLPAVTYEEMLELASVGAKVLQTRSVGLA
                     MREKIPIRVLSSFDDRPGTMVVGDEAIEEFNMERQLITGIAYDKNEARVTLTGVPDRP
                     GSVAAIFAPLAEGNINVDMIVQSVPRAGQKSDLTFTVPTASLAHSRDLLEKAREAVGF
                     DEMLTDTNVVKVSAVGVGMRSNAGIAARMFQALADRGINILAITTSEIKVSVLIAEEY
                     TELAVRVLHTAYGLDEMGAEG"
     gene            2498..>3030
                     /gene="ubiG"
                     /locus_tag="VF547_08490"
     CDS             2498..>3030
                     /gene="ubiG"
                     /locus_tag="VF547_08490"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX8262896.1"
                     /translation="MSDTSTIDPREAAHFGAMAGDWWDPNGSSAMLHKLNPVRLAYIR
                     DQVDQHWGLDECSLRPLEGKRAADVGCGAGLLAEPLARLGARVTGIDPAPENIAAARE
                     HALGQGLEIDYRVGGVEALDGRFDLVTSLEVVEHVAETRTFVQGLADALAEDGLLILS
                     TPNRTAMSRLMLILIGEG"
BASE COUNT          449 a         1042 c         1059 g          480 t
ORIGIN      
        1 acgaccggat gtgccgaacg ccccgttaag cagggatccg gcggtttgcc tcaggcgtgg
       61 acggcacgcc gctccagcgc ggccgcggcc tgatcgacga gcgagcggag gatgtcggcg
      121 accggctcct ccttcgtcac cattccgacg gattggcccg ccatgaccga gcccgtctcc
      181 acgtcgccct cgatcaccgc ccggcggagg gcgccggccc agtaatgttc gatctggagc
      241 tgggcctcgg ccatctcgac ctcgccccgg tcgagcaaat cggcgacctc gcgctgcttg
      301 gcggcgaagc tctccatctc gcggttcttg agggcgcgca cggggatgac ggggagccgc
      361 gggtcgatct gcacgctggt caccgcgtcg cgggccgagg cgcggatgaa cgctttcttg
      421 aagttcggat gggcgatgca ctcgtgcgcg cagacgaagc gggtgccgag ctggacgccg
      481 accgcgccca tctcgagata gccggcgatg aggccgccat ggccgattcc gccggcgacg
      541 aagatcggga tctccgcggc cagcggcggc aagatctcct gggcgagcac cgaggtcgac
      601 accgggccga tatggccgcc cgcctccatc ccctcgatga ccagcgcgtc tacgccggag
      661 cggaccagct tcttcgccaa ggcgagcgcc ggggcgaagc agatcagctt ggcgccattg
      721 cccttgatcc ggtcgatcga cccgggcggc ggcagcccgc cggcgagcac gacgtggctc
      781 acccggtggc gcgcgcagac gtcgatcagc tcggagagct gcggatgcat ggtgatgagg
      841 ttgaccccga acggccgctc ggtccgcaag cgggtctcgc cgatctcggt gtcgagcagg
      901 tcgggcgtca tcgccccgca ggcgatcacc ccgaacgcgc cggcgttgga cagggccgag
      961 acgaggttgc gctccgagat ccagctcatc gccccgccga ggatggcgac ctcgcagccg
     1021 aggaagtcgg tgccgcgctt catcagtcgg cggaggcggt cgcggccgac ggaattcgct
     1081 tcggtatcgg tcatcttctc tccgtcaccc cagcgaaggc tggggtccat gctggctcag
     1141 ggcggggcga ggaatccgaa tggatgccag ccttcgctgg catgacgctc acccctccgc
     1201 tcccatctcg tcgagcccgt aggccgtatg aagcacccgg accgcgagct cggtatattc
     1261 ctccgcgatc agcaccgaga ccttgatctc cgaggtggtg atggcgagga tgttgatgcc
     1321 gcggtcggcc agcgcctgga acatcctcgc ggcgattccg gcgttggagc gcatcccgac
     1381 cccgaccgcg ctgaccttga cgacgttggt gtcggtcagc atctcgtcga agccgaccgc
     1441 ctcgcgggcc ttctcgagca ggtcgcggct gtgggcgagc gaggccgtcg gcacggtgaa
     1501 ggtgaggtcg ctcttctggc cggcgcgcgg caccgactgg acgatcatgt cgacgttgat
     1561 attgccctcg gcgagcggcg cgaagatggc ggcgaccgag ccgggccggt cgggcactcc
     1621 ggtcagggtc acccgcgcct cgttcttgtc gtaggcgatg ccggtgatga gttggcgttc
     1681 catgttgaat tcctcgatgg cctcgtcgcc gaccaccatc gtgccggggc ggtcgtcgaa
     1741 cgacgagagg actcggatcg gaatcttctc gcgcatggcg aggccgaccg agcgggtctg
     1801 gagcaccttg gcgccgacgc tggcgagctc gagcatctcc tcgtaggtga cggcgggcag
     1861 cttgcgcgcc cgcggcacta tgcgcgggtc ggtcgtgtag accccgtcga cgtcggtgta
     1921 gatgtcgcag cggtcggcgt tgaccgccgc cgccagggcc actgccgacg tgtccgatcc
     1981 gcctcggccg agcgtcgcta ggctgccgtc ggcggcgacc ccctggaagc cggggatgac
     2041 ggcgatgccg ccctcgccga atacgcgctc gagcaccgtc gcgtcgatgt cgtcgatcag
     2101 cgccgaggcg tggccggaag cccggatcgg cagctgccag cccatgtagg agcgggcgtt
     2161 cagccccatc ccctggagcg tgatcgcgag cagcccgctc gtcacctgct cgccggacgc
     2221 caccaccacg tcatattcgc gcggatcgta gaggctggac gcctcgcggc agaactggat
     2281 cagccggtcg gtttcgccgg ccatggccga gacgacgacc gccacctggt tccccgcctc
     2341 ggcctcgtcc ttcacccttt gcgcgacatg ccggatccgc tcgatgcccg ccatcgaggt
     2401 gccgccgaat ttcatcacga tgcgggccat gacggcggct cttttcgtgt cgcggcgttg
     2461 agtgcaagga gattgtggca atcgacagga cccgggcatg agcgacacga gcacgatcga
     2521 cccccgcgaa gccgcccatt tcggcgcgat ggccggcgac tggtgggacc ccaatgggtc
     2581 gtcggcgatg ctccacaagc tcaacccggt gaggctcgcc tacatccgcg accaggtcga
     2641 ccagcattgg gggctggacg aatgcagcct gcggccgctc gagggcaagc gagcggccga
     2701 cgtcggctgc ggcgccgggc ttctcgccga gccgctcgcc cggctggggg cccgggtgac
     2761 ggggatcgac ccggcgccgg agaacatcgc cgccgcgcgc gagcatgcgc tcggccaggg
     2821 gctcgagatc gactatcgcg tgggtggggt cgaggccctc gacggccggt tcgacctcgt
     2881 cacctcgctc gaggtggtcg agcacgtcgc cgagacccgg acgttcgtcc agggcctcgc
     2941 cgacgccctt gccgaggacg gcctgctcat cctctccacc ccgaaccgca ccgccatgtc
     3001 gaggttgatg ctgatcctga tcggcgaggg
//
LOCUS       DASVQZ010000309         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_64157, whole genome shotgun sequence.
ACCESSION   DASVQZ010000309 DASVQZ010000000
VERSION     DASVQZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64157"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VF547_08495"
     CDS             complement(<1..418)
                     /locus_tag="VF547_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262897.1"
                     /translation="MEPSDRRDVLAGLAALPLVPLLACARGPEAAAARARLIAGSACP
                     VTPRQTEGPFYFDPKRVRPDVREGRPGLPLRLRLQVVTAAGCAPVAGARVDIWHCDAA
                     GTYSGYGSERSAGEAWLRGTQFADVDGVVAFETIYPG"
     gene            506..1543
                     /gene="obgE"
                     /locus_tag="VF547_08500"
     CDS             506..1543
                     /gene="obgE"
                     /locus_tag="VF547_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX8262898.1"
                     /translation="MHFLDQAKIYVRSGAGGPGAVSFRREKYIEYGGPDGGDGGKGGD
                     IVFEAVEGLNTLIDFRYTQHFRAPRGKGGSGSNRTGAGGDDLVIKVPVGTQILSEDKE
                     HVLADFTRVGQRQVFLKGGDGGRGNASYKSATNRAPRQHGTGWPGEEAWVWLRLKLLA
                     DVGLVGLPNAGKSTFLNAVTNAHAKVGAYPFTTLHPKLGVVRHRDREFVVADIPGLIE
                     GAAEGAGIGDRFLGHIERCRVLLHLVDSEQEDVAETYRTVRSELEAYGAGLADKPEVL
                     ALNKSDTLDEELTAALSDELEAEAGVRPLPLSGATGAGATEVLDALLPHVAAAKEADR
                     DEEETGSWSPL"
     gene            1681..2610
                     /locus_tag="VF547_08505"
     CDS             1681..2610
                     /locus_tag="VF547_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8262899.1"
                     /translation="MSKSRKIRTLAITGGTGFVGSHLLRLALAEGYEVRALTRGWKPP
                     EDDVAWVDGALDRPDTLAKLAAGADAIVHVAGAINARDRTGFEAVNVAGTAAMIDAAR
                     KAGVKRFVHISSLAAREPELSDYGWSKAKSERLVAASGLDWTIVRPPAVYGPGDRETL
                     ELFRMARRGLVALPPHGRFSVIHVEDLGRLILAVLDDPDSWSETYEPDDGREGGWQHR
                     HFARTLGRLYGRRALTLAMPRPVLRLASGLDRLVRRGNAKLTRDRVRYFCHPDWVATA
                     EKRPPGRLWQPRIKTATGLKATADWYREQGWLK"
     gene            complement(2749..3297)
                     /locus_tag="VF547_08510"
     CDS             complement(2749..3297)
                     /locus_tag="VF547_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262900.1"
                     /translation="MATNTKPADGALTLVEMKEFAGFPAATQRYIRRSLDVGLGRRDA
                     LRRWSRDVGEAASIRAQERMYRRLDHVRANVPDDSGLDEMEPVMGPLVTMTAFDLGQD
                     RLPDFASYRFLYERLIGAAVRPWLPGAFCAAAALPHLHPERRRALLQSISEAAATAPG
                     WSNREPVFFPEWVDKVDVTVAG"
     gene            3671..>4563
                     /locus_tag="VF547_08515"
     CDS             3671..>4563
                     /locus_tag="VF547_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8262901.1"
                     /translation="MESFADYRAHRRLPDLPLAAASILGFWLFYFATIVVRTLLIDES
                     LAWLGYRALGCVIGVVLTFLVYLVLSRAARESVRIQIVAAAIACVPAAAIFSTFNLAF
                     AVQQPLVERTVIRQVPGGPTIVRSIPRDEVRIGQNGVQIVTPLAGDPERRRQQVLRLI
                     ADGLVTWYFFFAAWASFYIAISSATRLRAAERRAAGAERDAQAAQLRALRYQVNPHFL
                     FNTLNSLSSLVMARRDEEAEAMIVNLSTFFRSSLALDPSADVTLAEEIEFQKLYLDIE
                     KVRFPRRLEVRVDVPDDLRSAR"
BASE COUNT          699 a         1681 c         1533 g          650 t
ORIGIN      
        1 agcccggata gatcgtctcg aacgccacca ccccatcgac gtcggcgaac tgggttccgc
       61 gcagccaggc ctcgccggcg ctacgctccg aaccatagcc ggaataggtt ccggcggcgt
      121 cgcaatgcca gatgtcgacc cgtgccccgg cgacgggggc gcagccggcg gcggtcacca
      181 cctggaggcg gaggcgcagc gggaggcccg gtcgcccttc cctcacgtcg ggccggaccc
      241 gcttcgggtc gaaatagaag ggaccctcgg tctgccgcgg agtcaccggg caggcgcttc
      301 ccgcgatcag ccttgcccgc gccgctgccg cctccggccc gcgcgcgcag gcgagcagcg
      361 gcaccagcgg caaagcggca aggccggcca gcacgtcgcg gcggtcgctg ggctccatcg
      421 cgccaggatc ggccgccgtc ccttaaccgc cgctgacgaa ggccgggccg cttgattccc
      481 cgccgcttgc gcctaaaccg ccgccatgca cttcctcgac caggccaaga tctacgtccg
      541 ctcgggcgcc ggcgggcccg gcgcggtcag cttccggcgc gagaaatata tcgaatatgg
      601 gggcccggac ggcggcgacg gcggcaaggg cggcgacatc gtcttcgagg cggtggaggg
      661 cctcaatacc ctgatcgact ttcgctacac ccagcatttc cgcgcgcctc ggggcaaggg
      721 cggctcgggc tccaaccgaa ccggcgccgg cggcgacgac ctcgtcatca aggtgccggt
      781 cggaacccag atcctgtcgg aggacaagga gcacgtgctc gccgacttca cccgggtcgg
      841 ccagcgccag gtgttcctga aggggggcga cggcggccgc ggcaatgcca gctacaagag
      901 cgcgaccaac cgcgcgccgc gccagcacgg caccggctgg ccgggcgagg aggcctgggt
      961 gtggctccgc ctgaagctgc tcgccgacgt cggcctggtc ggccttccca atgcgggcaa
     1021 gtcgaccttc ctcaacgccg tgaccaacgc gcatgccaag gtcggcgcct atccgttcac
     1081 gaccctccat cccaagctcg gggtcgtccg ccaccgcgac cgcgagttcg tggtcgccga
     1141 catacccggc ctgatcgagg gcgcggcgga aggcgccgga atcggcgacc gcttcctcgg
     1201 ccatatcgag cgctgccggg tcctccttca tctcgtcgat tcagagcagg aggacgtggc
     1261 ggagacctac cggacggtcc gctccgagct cgaagcctat ggggcgggcc tcgccgacaa
     1321 gccggaggtg ctcgcgctca acaagtccga cacgctcgac gaggagctga ccgccgcctt
     1381 gtcggacgag ctcgaggccg aggcgggcgt ccgtcccctg cccctctccg gagccaccgg
     1441 cgcgggggcg accgaggtgc tcgacgcact gctcccccat gtcgcggccg cgaaggaggc
     1501 ggatagggac gaggaggaga caggctcatg gtcgccgctc taggctcctc cccggaacgg
     1561 ggagggggac catgcgaagc atggtggagg gggccccgca ctgtcactgc gaagcggtgg
     1621 gccccctcca ccggcctgcg gccggtcccc ctccccgttc cggggaggaa ttagggcgcc
     1681 atgtccaaat cccgcaagat ccgcaccctc gccatcaccg gcggcaccgg cttcgtcggc
     1741 tcgcacctgc tgcgtctggc gctcgccgag ggctatgagg ttcgcgccct gacgcgcggc
     1801 tggaagccgc cggaggacga cgtcgcctgg gtcgacggcg ccctcgaccg gcccgacacc
     1861 ctcgccaagc tcgccgccgg cgcggacgcg atcgtccatg tcgccggggc gatcaacgct
     1921 cgcgaccgga ccggcttcga ggccgtcaac gtcgccggca ccgcggcgat gatcgacgcc
     1981 gcccgcaagg cgggcgtgaa gcgcttcgtc cacatctcct cgctcgcggc gcgcgagccg
     2041 gagctctccg attacggctg gtccaaggcg aagtcggagc ggctggtggc cgcctcgggc
     2101 ctcgactgga cgatcgtccg cccgcccgcc gtctacggcc ccggcgaccg cgagaccctc
     2161 gaattgttca ggatggcgag gcgcggcctg gtggcgcttc cgccccacgg ccgcttttcg
     2221 gtcatccatg tcgaggatct gggccgcctc atcctggccg tgctcgacga tcccgacagc
     2281 tggtccgaga cctacgagcc cgacgacggc cgcgagggcg gctggcagca tcgccatttc
     2341 gcccgcaccc tgggccggct ctacggccgc cgggcgctca ccttggccat gccgaggccg
     2401 gtgcttcgcc tcgcctcggg gctcgaccgc ctcgttcggc gcggcaacgc caagctcacc
     2461 cgcgaccggg tccgctattt ctgccacccc gactgggtgg cgaccgccga gaagcgtccc
     2521 cccgggcgcc tctggcagcc gcggatcaag accgccaccg ggctcaaggc caccgccgac
     2581 tggtatcgcg agcagggctg gctgaaataa tccgccgccg tgatattcgg tgccaggcac
     2641 cgaacgctaa ggggttgaat cgaaacggcg ggcactgagg cccgccgtcc gcaatatcaa
     2701 gaagccgata tgccgccccg atcagcggcc ccgatcagcc ggcggcgatc agccggcgac
     2761 ggtgacgtcg accttgtcga cccactcggg gaagaagacc ggctcgcggt tcgaccagcc
     2821 gggcgccgtc gccgccgcct cgctgatcga ctggagcaag gcgcggcgcc gctccgggtg
     2881 gaggtgcgga agcgcggcgg ccgcgcagaa ggcgccgggc agccatggcc gcaccgcagc
     2941 cccgatcagc cgctcgtaca aaaagcggta ggaagcgaaa tcgggcagcc ggtcctggcc
     3001 gaggtcgaag gcggtcatgg tgacgagcgg gcccatcacc ggctccatct cgtcgaggcc
     3061 gctgtcgtcg ggaacgttcg cgcggacatg gtcgagccgc cggtacatgc gctcctgcgc
     3121 ccggatcgag gcggcctcgc cgacgtcgcg cgaccagcgc cgaagcgcgt cgcgacggcc
     3181 gagcccgacg tcgagcgacc ggcggatgta gcgctgggtc gccgcgggga agcccgcaaa
     3241 ctccttcatc tcgaccaaag tcagcgcacc gtcggccggc ttggtgttcg tcgccatgag
     3301 tccactccca ctctttcacc cggcgggatc atgcgattca aacggttacc agccgcttaa
     3361 ccaagacccc gctccccatt cagaaatgaa tcaggtttca gggggagcgg caatgggaaa
     3421 aattcacgag cgcgagaggg gcggcgcagt gtagcttccg agccacaggc ccgatccgtg
     3481 aatcccagcg aaaccaaggg cttcgcggcg ccgaaacgac ttatccacag ctttctctcg
     3541 ccggcctctg cgcccaggga ggcccgcgag ccccgtccgg gcgcagattt cgaccaaagc
     3601 ggccgctccc tctgcgaacg acacgggcgg cggcgacaca aatgcgcaat tgcgggtaag
     3661 agggccgcgg atggagagct tcgccgacta tcgcgcgcac cggcgcctgc ccgacctgcc
     3721 gctcgcggcg gcgtcgatcc tcggcttctg gctgttctac ttcgcgacca tcgtcgtccg
     3781 caccctgctg atcgacgaga gcctcgcctg gctgggctat cgggcgctgg gctgcgtgat
     3841 cggggtcgtc ctgacctttc tcgtctatct cgtcctcagc cgcgccgccc gcgagagcgt
     3901 ccgaatccag atcgtcgcgg cggccatcgc ctgcgtcccc gccgccgcca tcttctcgac
     3961 cttcaacctc gccttcgccg tccagcagcc cctcgtcgag cggaccgtca tccgccaggt
     4021 gccgggcggg cccacgatcg tccggtcgat ccccagggac gaggttcgga tcggccagaa
     4081 cggggtccag atcgtcaccc cgctggcagg ggaccccgag cgccgccggc agcaggtgct
     4141 tcggctgatc gccgacgggc tcgtcacctg gtatttcttc ttcgccgcct gggcgagctt
     4201 ctacatcgcg atcagctcgg cgacccgcct gcgcgcggcc gagcgccggg ccgcgggcgc
     4261 cgagcgcgac gcccaggcgg cgcagctccg agcgctgcgc taccaggtca acccgcactt
     4321 cctgttcaac acgctcaact ccctgtcctc gctggtgatg gctcggcgcg acgaggaggc
     4381 ggaggcgatg atcgtcaacc tctccacctt cttccgctcg agcctcgctc tcgatccgag
     4441 cgccgacgtc actctggccg aggagatcga gttccagaag ctctacctcg acatcgagaa
     4501 ggtgcgcttc ccgcgccggc tcgaggtcag ggtcgacgtg cccgacgacc tgcgctcggc
     4561 gcg
//
LOCUS       DASVQZ010000310         5179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_834018, whole genome shotgun sequence.
ACCESSION   DASVQZ010000310 DASVQZ010000000
VERSION     DASVQZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834018"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="VF547_08520"
     CDS             <1..1820
                     /locus_tag="VF547_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX8262902.1"
                     /translation="QLWRHTPAQSSFPANMLAIRGGRPETLNLRDIIESFVRFREEVI
                     TRRTKFELAKARDRAHILLGLVIAVTNLDEVVRIIRGSASPAEARAALVARDWPVAEI
                     APYIALVEAVEHEVEGDSYRLSDAQVRAILELRLHRLTGLGRDEIAGELRQLATSIGE
                     LLEILGDRVKLYAVMRSEFDEIADQFATPRVTEIAPAWDGIEDEDLIEREDMVVTVTH
                     GGYIKRTPLDTFRAQRRGGKGRAGMATKDEDAITNLFVTSTHTPVLFFSSAGKVYRLK
                     VWRLPEGAPQARGRPMVNLLPLGEGETISTVLPLPEDEAEWGKLHILFATAHGTVRRN
                     SMDAFTNVPTAGKIAMRFGNDEEGDPTDRLIGVSLLTEEDDVLLATKQGKAIRFCATE
                     VREFQSRTAAGVRGIRLQEGDEVISLSILKGFEATSDEREAYLRAAPWKENENPPTLS
                     PERMAEFEAAEEFILTVTENGYGKRTSAYEYRRTGRGGQGITNIETSERNGCVVASFP
                     AHNGQQLMLVTDQAKLIRTTVGDIRIAGRSTQGVTIFRVAENEHVVSAARIDEDEEPE
                     TEAEEMIAEELGEAPPAAASDTVQLDDGTGPETLPDSQE"
     gene            complement(1927..2598)
                     /locus_tag="VF547_08525"
     CDS             complement(1927..2598)
                     /locus_tag="VF547_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HEX8262903.1"
                     /translation="MTAATRVLVALSLVFGLGYPLLAGAPLGPEATIAAKGAGVGLLA
                     LAASLRARDADRWLLASVMALGALGDILLEIDFAAGAAAFAAGHFVAIALYLRNPRPE
                     RVSRDLGVAVMLPALAVIIPLAMLDGRPEAWPFVLYALLLGTMAGSALLSRFPRRAVF
                     AGAMLFLVSDMLIAVRLGTGRAGFGLPIWLLYYLGQLMIFLGVTSSLPAEPVGRGTTR
                     SLVEG"
     gene            2639..4072
                     /gene="trmFO"
                     /locus_tag="VF547_08530"
     CDS             2639..4072
                     /gene="trmFO"
                     /locus_tag="VF547_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX8262904.1"
                     /translation="MTHQVHIIGGGLAGSEAAWQLAEAGVKVRLSEMRGSGETTPAHH
                     SDSLAELVCSNSFRSDDAEHNAVGLIHAEMRRLGSLIMACADAHRVPAGSALAVDREG
                     FAAGVTARIEAHANIEVVRERIDALPASGPTIVATGPLTAMKLAESIGAVTGSDALAF
                     FDAIAPIVHRDSIDMDVAWMAARWGRSARLSPGQAEDGQGPSSPNDGGKDYINCPMTK
                     AQYLAFHQGLLDGEKTEFKDWERSTPYFEGCMPIEVMAERGVDTLRFGPMKPVGLDDP
                     KTGRWPYAVVQLRQDNALGTLWNMVGFQTKLKHAEQIRLFRTIPGLEKAEFARLGGLH
                     RNTFINAPELLDPALRLKKAPHIRFAGQITGCEGYVESAAVGLLAGRFAAAELRGEAM
                     APPPPETALGALLGHITGGADSASYQPMNVNFGLFPPVDGKAKKADRKRAYTKRAEAA
                     LAQWLGAQDPPCREATGRGTMRSMVEG"
     gene            complement(4073..4510)
                     /locus_tag="VF547_08535"
     CDS             complement(4073..4510)
                     /locus_tag="VF547_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX8262905.1"
                     /translation="MLKWKILPIAQMGRGTMRSMVEGSMAKSSHARAGNAAVLRARRL
                     RRSMSPPEASLWQALRRRPESLKFRRQHPFERYTADFYCASAKLVVEIDGDSHGMGDN
                     PQRDVRRDAWMREQGLRVLRFSAEEVMRDLESVVRAILLTARR"
     gene            complement(4504..4938)
                     /locus_tag="VF547_08540"
     CDS             complement(4504..4938)
                     /locus_tag="VF547_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX8262906.1"
                     /translation="MIRIAVAGASALLLIAALFFVWKSRAEADDPVPPAPPPAARYSG
                     PGPRVTPPPAASPADRERKRFARADRNRDGRITLDEQLQPRRKAFARADRDGSGSLSF
                     EEWTAASAARFAKADKDGSGWLDAAEYEASRPKPKARPKCKC"
BASE COUNT          884 a         1861 c         1724 g          710 t
ORIGIN      
        1 accagctctg gcggcacacg ccggcgcaat cctccttccc ggccaacatg ctcgccatcc
       61 gcggcggccg gcccgagacg ctcaacctcc gcgacatcat cgagagcttc gtgcgcttcc
      121 gcgaggaggt gatcacccgc cggaccaagt tcgagctcgc caaggcccgc gaccgcgccc
      181 acatattgct cggcctggtc atcgccgtca ccaatctcga cgaggtggtg cggatcatcc
      241 gcggctcggc ctcgccggcc gaggcgcgcg ccgccctcgt cgcgcgcgac tggccggtgg
      301 ccgagatcgc gccctatatc gcgctggtcg aggcggtcga gcacgaggtc gagggcgaca
      361 gctaccggct gtccgacgcc caggtgcggg ccatactcga gctgcgcctg caccgcctga
      421 ccggcctcgg ccgcgacgag atcgccggcg agctgaggca gctcgccacc tcgatcggcg
      481 agctgctcga gatcctcggc gaccgcgtca agctctacgc cgtgatgcga tccgagttcg
      541 acgagatcgc cgaccagttc gccaccccgc gggtcaccga gatcgcgccc gcctgggacg
      601 ggatcgagga cgaggacctg atcgagcgcg aggacatggt cgtcaccgtc acccatggcg
      661 gctacatcaa gcgcactccg ctcgacacct tccgcgccca gcgccgcggc ggcaagggcc
      721 gggccgggat ggcgaccaag gacgaggacg cgatcaccaa tctgttcgtc acctcgaccc
      781 acacgccggt cctgttcttc tcgtcggccg gcaaggtcta ccgcctcaag gtctggcggc
      841 tgcccgaggg cgctccgcag gcgcgcggcc ggccgatggt caacctgctg cccctcggcg
      901 agggcgagac catctcgacc gtgctgccgc tgccggagga cgaggcggaa tggggcaagc
      961 tccacatcct gttcgccacc gcccacggca ccgtccgccg caactcgatg gacgccttca
     1021 ccaacgtccc gaccgcgggg aagatcgcga tgcgcttcgg caatgacgag gaaggcgatc
     1081 ccaccgaccg gctgatcgga gtctcgctgc tcaccgagga ggacgacgtc ctgctcgcga
     1141 ccaagcaggg caaggcgatc cgcttctgcg cgaccgaggt ccgcgaattc cagtcgcgca
     1201 ccgccgccgg cgttcgcggc atccgcctgc aggagggcga cgaggtgatc tccctgtcga
     1261 tcctgaaggg gttcgaggcg acgtcggacg agcgcgaagc ctatcttcgg gcggcgccct
     1321 ggaaggagaa tgagaacccg ccgacgctca gccccgagcg catggccgag ttcgaggcgg
     1381 ctgaggagtt catcctcacg gtcaccgaga acggctacgg caagcgcacc tccgcctacg
     1441 aatatcgccg caccgggcgc ggcggccagg gcatcaccaa tatcgagacg tcggagcgca
     1501 acggctgcgt cgtcgccagc ttcccggccc ataacggcca gcagctgatg ctggtcaccg
     1561 accaggccaa gctcatccgg acgacggtcg gcgacatccg gatcgccggc cgcagcaccc
     1621 agggcgtcac catcttccgg gtcgccgaga acgagcatgt cgtctccgcc gcccggatcg
     1681 acgaggacga ggagccggag acggaagccg aggagatgat cgccgaggag ctgggcgagg
     1741 cgccgccggc ggccgcgtcg gacacggtcc agctcgacga cggcaccggg ccggagactt
     1801 tgccggacag ccaggaataa atcctcccca tcgccagatg gggaggggga ccatgcaaag
     1861 cagggtggag gggggattga cgctggatcg ggcggatcct gcgcgaaccg tccgagctgg
     1921 gggcagctac ccctccacca gacttcgtgt ggtccccctc cccaccggct ccgccggcag
     1981 ggaggatgtg accccgagaa agatcatcag ctgcccgaga tagtagagca gccagatggg
     2041 caggccgaag ccggcccgcc cggtcccgag acgaaccgcg atcagcatgt cgctcaccag
     2101 gaacagcatg gcgccggcga agaccgcccg gcggggaaag cggctcagca gggcgcttcc
     2161 cgccatcgtg ccgagaagga gcgcgtagag gacgaagggc caggcttccg gccgcccatc
     2221 gagcatggcc agcggaatga tgaccgccag ggccggaagc atgacggcga cgcccaggtc
     2281 acgcgagacc cgctccgggc gcgggttgcg caaatagagg gcgatggcga cgaagtgtcc
     2341 ggcggcgaaa gcggcggcgc ctgcggcgaa gtcgatctcg agcaggatgt cgcccagcgc
     2401 tcccagcgcc atcaccgagg cgagcagcca tcggtccgcg tcgcgcgccc gcaaggaggc
     2461 cgcgagggcg agcaggccga cgccggcccc tttcgccgcg atcgtcgctt ccgggccgag
     2521 cggggcgccc gccagcaagg gatagcccag cccgaagacg aggctcaacg ccaccagcac
     2581 ccgcgtcgcc gccgtcatcc gcttcctccc cgctccaaag cggactatcc caccaccgat
     2641 gacccaccaa gtccatatca tcggcggcgg cctcgccggc tcggaagccg cctggcagct
     2701 ggccgaagcc ggagtgaagg ttcgcctgtc ggagatgcgc ggctccggcg agacgacccc
     2761 ggctcaccac agcgacagcc tcgccgagct ggtctgctcg aacagcttcc ggtccgacga
     2821 cgccgagcat aacgccgtcg gcctcatcca cgccgagatg cgccgtctgg gctcgctgat
     2881 catggcttgc gccgacgccc accgggtgcc cgccggctcg gcgctggcgg tcgaccgcga
     2941 aggcttcgcc gccggcgtca cggcgcggat cgaggcccat gccaatatcg aggtcgtccg
     3001 cgagcggatc gatgccctcc ccgcctccgg cccgaccatc gtcgccaccg gtccgctgac
     3061 cgcgatgaag ctggcggaat cgatcggcgc cgtcaccggc tccgacgccc tcgccttctt
     3121 cgacgccatc gcgccgatcg tccacaggga ctcgatcgac atggacgtcg cctggatggc
     3181 ggcgcggtgg ggtcgttcgg cccgcctgtc ccccggacag gctgaagacg gccaggggcc
     3241 gtcttcaccg aacgacggcg gcaaggacta catcaactgc ccgatgacga aggcgcagta
     3301 tctcgccttc caccagggcc tgctcgacgg cgagaagacc gagttcaagg actgggagcg
     3361 cagcacgccc tatttcgaag gctgcatgcc gatcgaggtg atggccgagc gcggcgtcga
     3421 caccttgcgc ttcggcccga tgaagccggt cgggctcgac gatccgaaga ccgggcgctg
     3481 gccctatgcc gtcgtccagc tccgccagga caatgcgctg ggcaccttgt ggaacatggt
     3541 cggcttccag acgaagctca agcatgccga gcagatccgc ctgttccgga ccattccggg
     3601 gcttgagaag gccgagttcg cgcggctggg cggactccac cgcaacacgt tcatcaacgc
     3661 gcccgagctg ctcgatccgg cgcttcggct gaagaaggcg ccgcacatcc gcttcgccgg
     3721 ccagatcacc ggctgcgaag gctatgtcga atccgccgcc gtcggcctcc tcgccggccg
     3781 cttcgccgcc gccgagctgc gcggcgaggc gatggcaccg cccccgcccg agacagcgct
     3841 cggcgccctc ctcggccaca tcaccggcgg cgccgattcg gccagctacc agccgatgaa
     3901 cgtcaatttc ggcctgttcc cgccagtcga cggcaaggcg aagaaggcgg accggaaacg
     3961 ggcctatacg aagcgggcgg aggccgcgct ggctcagtgg ctcggcgccc aagatcctcc
     4021 ctgccgcgaa gcgacgggga gggggaccat gcgaagcatg gtggaggggt gactaacgcc
     4081 gagccgtgag aaggatcgcc ctaaccaccg attcaagatc cctcatcacc tcctcggcgc
     4141 tgaagcggag cacccgaagc ccctgctccc gcatccaagc gtcccgccgg acatcccgct
     4201 gcggattgtc gcccattcca tggctgtcgc catcgatttc cacgacaagc ttcgcagagg
     4261 cacagtaaaa atccgccgta tagcgctcga acggatgctg ccgccggaac ttcagacttt
     4321 cggggcgacg gcgcaatgcc tgccaaaggg acgcttccgg cggcgacatc gagcgcctca
     4381 accggcgcgc ccgcaggacg gccgcgtttc cggcgcgagc atgtgaactc ttagccatcg
     4441 acccctccac catgcttcgc atggtccccc tccccatctg cgcgatgggg aggattttcc
     4501 atttcagcac ttgcacttcg ggcgggcctt gggcttcggc ctcgacgcct catattccgc
     4561 ggcgtcgagc cagcccgagc cgtccttgtc ggccttggcg aagcgggcgg cgctggcggc
     4621 ggtccattcc tcgaagctga ggctgccgct gccgtctcgg tcggccctgg cgaaggcctt
     4681 gcggcgcggc tgcaactgct cgtcgagcgt gatccggccg tcgcggttgc ggtcggcgcg
     4741 ggcgaagcgc ttccgctccc gatcggcggg cgaggcggcg ggcggcggcg tcacccgcgg
     4801 gccggggccg gaatagcgag cggccggcgg aggcgctggc ggaaccggat cgtcggcctc
     4861 ggcgcggctt ttccagacga agaacaaggc ggcgatcagc agaagcgcgg acgcgcccgc
     4921 gacggcgatt cgaatcatgg gctcctcctc cctggaccga ggaaccataa ccgccggaaa
     4981 gcgcaattct aagcgtcgat tttccgcaaa gcgctcaata tccagtaatt ctatgccgca
     5041 acatccgcca catgcgtgcg ggcgcgcccc tcggctccca gagcggtcgg ttggggtcca
     5101 agtcccggtc ggcgagcgcc gccagcatgc ccagcggccg gaggcgcggc ggcctgctgt
     5161 tccattacac cgcccgcct
//
LOCUS       DASVQZ010000311         9959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_930576, whole genome shotgun sequence.
ACCESSION   DASVQZ010000311 DASVQZ010000000
VERSION     DASVQZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930576"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VF547_08545"
     CDS             <1..821
                     /locus_tag="VF547_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8262907.1"
                     /translation="LADLGPFGNLAAGPGNGDFDVAEIGLQLFGPVVRFAGAGGIEAL
                     FLDLIASARRFVYIETQYFASRVVAEAIGKRLAEPDGPEFVIVNPKAGYGWLDESVMG
                     PARAELVKALATKDRYGRFQIYTPVTEGGADIYVHAKIMIVDDAAIRVGSANLNNRSM
                     GLDSECDLMIDARLPGNEAAGPAISRLRADLIAEHLGVDPAEVAALCDGTGSLIATIE
                     RLRGTGRTLLPYRPPEFSAPLKAVAKSEMLDPESACDPFEPRARHRLLAGFRRR"
     gene            896..2092
                     /locus_tag="VF547_08550"
     CDS             896..2092
                     /locus_tag="VF547_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8262908.1"
                     /translation="MRGLDIGVAGCGIGGLAAALALARDGHRVRLYERFDAPRPVGSG
                     LMIQPTGLAVLDRLGLAAPLAAAAAPIERLLGRAEPSGRVVLDVRYEWLRRPGARGFG
                     VHRATLFDILHRASAAEGIPIETGRTVAGREGRRLLFADGGRSDAHDLLVDALGSGSP
                     LAGRHARPLAYGALWASLDWTEGFDAGALEQRYRRASVMTGVLPVGLAPAGTRRQAAF
                     FWLLRGDRLSAWRERGLDPWKAEVRALWPATAPLLDQIASPDRLTFARYAHRTLREPA
                     SPGLIHIGDSWHSTSPQLGQGANMALLDAWALATALREEGDVEAALRRAVALRRFHVR
                     LYQGLSALFTPAYQSDSRVLPWIRDRIVPPFGRLWPATRIQAEMVAGTFGGPLERLGL
                     APPSAS"
     gene            complement(2079..2444)
                     /locus_tag="VF547_08555"
     CDS             complement(2079..2444)
                     /locus_tag="VF547_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8262909.1"
                     /translation="MITHVKFVSIPSRDQDRALEFWTRQVGFTVLSDQPFSATQRWIE
                     LQVANSDTRLVLFTPEGQEDRIGAFFNGALACDDVEATYRQLSGRGVEFVSPPQKQPW
                     GTFATFKDPDGNQFVLSSR"
     gene            2524..2826
                     /locus_tag="VF547_08560"
     CDS             2524..2826
                     /locus_tag="VF547_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8262910.1"
                     /translation="MTPTLPLDPYLIDVLMRDLVGHDRRPAAFVVYLWLWRNSRGEGR
                     PRVAASLQTLATATGLSKSAVQAAVRHLTHRRLLSATRRHATDAPSWEVQEPWRRA"
     gene            complement(2823..3095)
                     /locus_tag="VF547_08565"
     CDS             complement(2823..3095)
                     /locus_tag="VF547_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEX8262911.1"
                     /translation="MADTPPDRLSLNPRSPFFDGELLRRGVGIRFKGKERTDVEEYSV
                     SEGWIRVAAGRSLDRYGQPMTVRLKGEVEPFFQDAKGDEDSGGDEA"
     gene            complement(3127..3306)
                     /locus_tag="VF547_08570"
     CDS             complement(3127..3306)
                     /locus_tag="VF547_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262912.1"
                     /translation="MAEPFARHRQPWKQEELQKLHLLAGKGMSLKAVAKALTRTEESV
                     KERAKLDGLKISKLR"
     gene            complement(3428..5284)
                     /locus_tag="VF547_08575"
     CDS             complement(3428..5284)
                     /locus_tag="VF547_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8262913.1"
                     /translation="MIALLLAGCATNSTKGEDLALPAAAEAAQPDAAADGGSAPTAGP
                     AAEDARLLEFLDSAFDEAAERSPETLTGLGIKRRYGELDRYTDEEAAADLALAERNMV
                     RMKAQFDPERLSPAGRLSYRLAEIELDYAREQFKWRSHRFPVSTNGSPAGGIPVFLIN
                     QHRVDTVADAEAYVSRLREVERVMGEVGARMRRQAGHGIVPPKLVFAPVRADARRVIA
                     GAPFDSGPDSTLFADFKKKVAALDAPADVKARLVADAAAALTGPFRRGYDIFFATLDS
                     IEPKAKGNDGAWSLPNGGDYYASRLRLSTTTDLSADRIHRIGLDEVRRIQGEMEAIKA
                     RVGFKGSLQQFFAHIKTGARFKYPNTDAGRAQYLADAKAYIAQVMAKAPQWFSRLPKA
                     PLEVRAVETWRQETASVAFFNPPTPDGSRPGIFYVNLADMNQVLKPQTESIAYHEGAP
                     GHHFQIALQQELQGIPKFRRFGGYGAYIEGWGLYAERLGREMGFFQDPYSEFGHLSLE
                     LWRAVRLVTDTGLHSKRWSREQAIRYFQENSLLSERDIVKEVERYINNPGQATSYKIG
                     QLRIVELRERAKRALGDRFDIRAFHAAVLENGALPLDLLDAQVQAYIDANRS"
     gene            complement(5334..5813)
                     /locus_tag="VF547_08580"
     CDS             complement(5334..5813)
                     /locus_tag="VF547_08580"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX8262914.1"
                     /translation="MTNDACGGCGRAVEGGTAGCRAAFEALLARDFSDARFFGVHRLF
                     VDCYCLQHPDDYCASAKSLAAHLVGLAQIVEQDASPATGSPGLRNWLDGDRALDKPAL
                     PAERGAMTLGDLAEIGDPAAWREAVRKWAASIWAAYRDLQPLARRWAGEAGRAAPRR"
     gene            complement(5810..6736)
                     /locus_tag="VF547_08585"
     CDS             complement(5810..6736)
                     /locus_tag="VF547_08585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8262915.1"
                     /translation="MQRSSVDLLDVLAFVRVAETGAFSRAAERMGMSKSILSRRVARL
                     EAQLGARLLTRTAQGAQPTEVGQAYFERAANILAELEAAEEVVAEAVTQVAGPIRLSA
                     PLSFGIGHLAPALAEFAEANPRVELDISFDDRPVDLAAGGFDLAVRIGSLKDSALVAR
                     RIAPVRKVPTASPAYLEARGRPEHPRDLAGHDILLYANEQWRFRVGGKWEYVRGRPRL
                     RADNGEMLRAAAEAGLGVCILPSFIAAPAFERGTLEPLLRDYPLEEGGLHAVMPPGRA
                     TTARVRALVDFLAARFGPEPQWDPCWLAGERR"
     gene            6866..7363
                     /locus_tag="VF547_08590"
     CDS             6866..7363
                     /locus_tag="VF547_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262916.1"
                     /translation="MTNKLNRPLLAAVLPAAMLIPAAPAAAQGSPTYEYAAKFVCAAS
                     SNLTPPVAVGHYYTAVNVHNPGIDVKVAWKVALAGTILPGKITGFDSQLLKNDEAMEI
                     DCRSIRNRITASGLPNPAAFFTGFVVIRAPAPVDVVSVYTASAQVNGPVVSIHTERVP
                     ARQLP"
     gene            7462..8088
                     /locus_tag="VF547_08595"
     CDS             7462..8088
                     /locus_tag="VF547_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEX8262917.1"
                     /translation="MTNVLIIDSAATGDASVSRRLTGELEAILRGRGAGRIVRRDVGT
                     ETIPHLTAATTAAIRGAPAETDAAREAIALSDTLIAELKAADLVVIGAPMYNFGMPST
                     LKAWFDHVLRAGITFRYSQTGPEGLVKGKKAIVIESRAGLYSEGPAAAMDSQEPHLRT
                     LLGFMGLDDVAFVRVEKLAFGPDQEAASFAEAIEELRSLAADDLPLAA"
     gene            8173..8781
                     /gene="gstA"
                     /locus_tag="VF547_08600"
     CDS             8173..8781
                     /gene="gstA"
                     /locus_tag="VF547_08600"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX8262918.1"
                     /translation="MKLYYAPGACSQAPHIVAREAGLDIELANVRIPDKVTDDGEKLT
                     DVNPKGAVPTLRLDDGEVLTENAVILQYLADRAEGSELALPASGIERYRLLEWLNFIA
                     TELHKGFGPLWNPATPDSFKEATREALGKKFDYLQEKIGDGPYILGERFSILDAYAFA
                     VLNWSAIHSIDIGRWPGLAAYLQRVAARPAVQETLRAEGLIE"
     gene            8872..9570
                     /locus_tag="VF547_08605"
     CDS             8872..9570
                     /locus_tag="VF547_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8262919.1"
                     /translation="MIDIRPFSTLGHADHGWLNARHHFSFADYHAPARMGWGAIRVWN
                     DDEIAARSGFPPHPHRDMEIITYVREGAITHQDSMGNKGRTAAGDVQVMSAGTGVRHA
                     EYNLEDETTTLFQIWVQTDKPGAEPAWGARQFPRDGRAGNFVTLASGYAEDGDALKIN
                     AAARVLGATLNSGETAELELDPARHVYLVAVGGAIEVNGWRAEPRDGIAVTGEEKLAI
                     RAMENAEIVLVDAK"
     gene            complement(9584..>9959)
                     /locus_tag="VF547_08610"
     CDS             complement(9584..>9959)
                     /locus_tag="VF547_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8262920.1"
                     /translation="RPRHELEFQATAFKRGLGARVKVNWQSGTTVSGLPAGLAGSSED
                     LIFSDHATVTINLFANLADHFGGETAPGWLKRTRVSLGINNLFNRRPEVRDEAGSIPL
                     SYQPAFLDPLGRSLSFSLRKIF"
BASE COUNT         1419 a         3628 c         3366 g         1546 t
ORIGIN      
        1 atctcgcgga cctcggacca ttcgggaacc tcgccgcggg tccgggcaat ggcgatttcg
       61 acgtcgcgga aatcgggctc cagctcttcg ggccagtggt ccgcttcgcc ggggccggcg
      121 gaatcgaggc gcttttcctc gatctgatcg cgagcgcccg ccgcttcgtc tacatcgaga
      181 cccaatattt cgcctcccgc gtcgtcgccg aggcgatcgg caagcgcctg gccgagccgg
      241 acggccccga attcgtcatc gtcaacccga aggcgggcta tggctggctc gacgaatcgg
      301 tgatgggccc ggcgcgcgcg gagctggtca aggcgctcgc gacgaaggac cgctacggac
      361 gcttccagat ctacacgccg gtgaccgagg gcggcgccga catctatgtc cacgccaaga
      421 tcatgatcgt cgacgacgcc gccatccgcg tcggctccgc caatctcaac aaccggtcga
      481 tgggcctcga cagcgagtgc gacctgatga tcgacgcccg ccttcccggc aacgaagcgg
      541 ccggcccggc catctcccgg ctgcgcgccg acctcatcgc cgagcatctc ggagtcgatc
      601 ccgccgaggt cgccgccctg tgcgacggga ccggttcgct gatcgccacg atcgagcgcc
      661 tgcgcggcac cggccgaacg ctcctcccct accgcccgcc cgagttcagc gccccgctga
      721 aggcggtcgc gaagagcgaa atgctcgatc cggagagcgc ctgcgacccc ttcgagccgc
      781 gcgctcgcca tcgcctgctc gccggcttcc gccgccgctg atcaggcccg ttcggccgcg
      841 ccgggaattc gactaaccga ttcaagtcgg cggtccatcg cgctacagcc gctccatgcg
      901 gggactggac atcggcgtcg cggggtgcgg gatcggcggc ctggcggccg cgctggcgct
      961 ggcccgcgac ggccaccggg tccgcctcta cgagcggttc gacgcgcccc gccccgtcgg
     1021 gtccgggctg atgatccagc cgaccggcct cgccgttctc gacaggctcg gcctcgccgc
     1081 cccgctcgcc gccgccgccg cgccgatcga gcggctgctc ggccgggccg agccgtcggg
     1141 ccgggtcgtg ctcgatgtcc gctacgaatg gctgcgccgg ccgggcgcgc gcggcttcgg
     1201 ggtccatcgc gcaaccctgt tcgacatcct ccaccgggcc tccgccgccg agggcattcc
     1261 gatcgagacc ggccggaccg tcgccggccg ggaagggcgg cgcctgctct tcgccgacgg
     1321 cggacgttcc gacgcccacg acctcctcgt cgacgccctc ggaagcggct cgcccctcgc
     1381 cggccgccac gcccggccgc tggcctatgg cgcgctctgg gcgagcctcg actggaccga
     1441 gggtttcgat gcgggcgcgc tcgagcagcg ctatcgccga gccagcgtca tgacgggcgt
     1501 gctgccggtc ggcctggcgc ccgccggcac ccggcgccag gccgccttct tctggctgct
     1561 tcgcggggac cggctgtccg cctggcgcga gcggggcctc gatccctgga aggcggaggt
     1621 tcgtgcgctg tggcccgcga ccgctcccct gctcgaccag atcgcaagcc cggaccgcct
     1681 caccttcgcc cgctacgccc atcgcaccct gcgcgagccc gcctcgcccg gcctcatcca
     1741 catcggcgac tcctggcact cgaccagccc gcagctcggc cagggcgcca acatggccct
     1801 gctcgacgcc tgggctctgg cgacggccct gcgagaggaa ggcgacgtcg aggccgcgct
     1861 gcgccgcgcg gtcgcgctcc ggcgcttcca cgtccgtctc taccagggcc tctcagccct
     1921 cttcaccccg gcctaccagt ccgacagccg ggtcctcccc tggatccgcg accggatcgt
     1981 cccgcccttc ggccgcctct ggccggcgac ccggatccag gcggagatgg tggccgggac
     2041 gttcggcggt ccgctggagc ggctcgggct cgcgccgcct agcgcgagct gagcacgaac
     2101 tggttgccgt ccgggtcctt gaacgtagcg aacgttcccc agggctgctt ctgcgggggc
     2161 gagacgaatt cgacgccgcg cccgctcagc tggcgatagg tcgcctcgac gtcgtcgcag
     2221 gcgagcgcgc cgttgaagaa ggcgccgatc cggtcctcct gcccctcggg cgtgaacagg
     2281 acgaggcgcg tgtcgctgtt cgccacctgg agctcgatcc accgctgagt cgcgctgaac
     2341 ggctggtcgc tgaggaccgt gaagccgacc tgccgggtcc agaattccag cgccctgtcc
     2401 tggtcgcggc tggggatgct gacgaacttg acgtgggtga tcatggcggg cctccgtggc
     2461 gcgatccttc gcccgccccg cccgccggcg acaaggggtt cgataaacac tatagtggcc
     2521 ggtatgacgc cgacgctccc gctcgacccc tatctgatcg acgtgctgat gcgcgacctg
     2581 gtcggccacg accgccgccc cgccgcgttc gtcgtctatc tgtggctgtg gcggaacagc
     2641 cgcggcgagg gccggcctcg cgtcgccgcc agcctgcaga ccctggccac ggccacgggc
     2701 ctctccaaat cggcggtgca ggccgccgtc cgccacctga cgcaccggcg gctgctcagc
     2761 gccacccgcc gccacgccac cgacgcgccg agctgggaag tgcaggagcc ctggcggcgc
     2821 gcctaggctt cgtcgccgcc gctgtcctcg tcgcccttgg catcctggaa gaagggctcg
     2881 acctcgccct tcagccgaac cgtcatcggc tggccgtagc ggtcgaggct gcggccggcg
     2941 gcgacccgga tccagccttc ggagacgcta tattcctcga cgtcggtccg ctccttgccc
     3001 ttgaagcgga tgccgacgcc gcggcgcagc agctcgccgt cgaagaacgg gctgcgcggg
     3061 ttgagcgaaa ggcggtccgg gggcgtgtcg gccattgcga tctcttcgtg gcgggcgggc
     3121 ctggggtcag cgcagcttgc tgatcttcag cccgtcgagt ttcgcgcgct ccttcaccga
     3181 ctcctcggtt cgggtcaatg ccttggccac cgccttcagc gacatcccct tgccggcgag
     3241 caggtgcagc ttctggagct cctcctgctt ccagggctgg cggtggcggg cgaagggctc
     3301 cgccatcaat ccttgaccac agttcccttg gtggacggat tggccgcgat ggtcttcttg
     3361 acctccttct tgggcttcct ggcttccttg ttgctcttct tctgaccctt ggccatcggc
     3421 tctctcctta gctccggttg gcgtctatat aggcttgcac ctgcgcgtcg agcaggtcga
     3481 gcggcaaggc gccgttctcg aggacggcgg cgtggaaggc gcggatgtcg aagcggtcgc
     3541 cgagcgcgcg cttcgcgcgc tcgcgcagct cgacgatcct cagctggccg atcttgtagc
     3601 tggtcgcctg gccgggattg ttgatgtagc gctccacctc cttgacgatg tcgcgctcgg
     3661 acagcagcga attctcctgg aagtagcgga tcgcctgctc gcggctccag cgcttggaat
     3721 gaaggccggt gtcggtgacg aggcgcaccg cccgccacag ctccaggctg aggtggccga
     3781 actccgaata ggggtcctgg aagaagccca tctcccggcc gagccgctcg gcatagaggc
     3841 cccagccctc gatataggcc ccgtagccgc cgaagcggcg gaatttcggg atgccctgca
     3901 gctcctgctg gagggcgatc tggaaatggt ggcccggggc gccctcgtga taggcgatgc
     3961 tctccgtctg cggcttgagc acctggttca tgtcggcgag gttgacgtag aagatgccgg
     4021 gccgcgagcc gtcgggcgtc ggcgggttga agaaggcgac cgacgccgtc tcctgccgcc
     4081 aggtctccac cgcccgcacc tcgagcgggg ccttgggtag ccggctgaac cattgcggcg
     4141 ccttggccat cacctgggcg atataggcct tggcgtcggc cagatattgg gcgcggccgg
     4201 cgtcggtatt gggatatttg aaccgggcgc cggtcttgat gtgggcgaag aattgctgga
     4261 gcgacccctt gaagccgacc cgcgccttga tcgcctccat ctcgccctgg atgcgccgga
     4321 cctcgtcgag gccgatccgg tggatccggt cggcgctgag gtcggtcgtc gtcgagagcc
     4381 gcagtcggct ggcataatag tcgccgccgt tgggcaggct ccaggcgccg tcattgccct
     4441 tcgccttcgg ctcgatcgaa tcgagcgtcg cgaagaagat gtcatagccg cggcggaacg
     4501 gcccggtcag ggccgccgcc gcgtcggcca ccagccgcgc cttgacgtcg gccggcgcgt
     4561 cgagcgcggc gaccttcttc ttgaagtcgg cgaagagggt gctgtcgggg cccgagtcga
     4621 acggcgcgcc ggcgatcacc cgccgggcgt cggcccgaac cggtgcgaag accagcttgg
     4681 gcggcacgat cccatgcccg gcctgcctgc gcatgcgcgc gccgacctcg cccatcaccc
     4741 gctcgacctc cctcaggcgg gacacatagg cttcggcgtc ggcgaccgtg tcgacccggt
     4801 gctggttgat caggaagacg gggatgccgc cggccgggct gccgttggtc gagaccggga
     4861 agcggtgcga gcgccatttg aactgctcgc gggcatagtc gagctcgatc tcggccagcc
     4921 ggtagctgag ccggccggcc gggctcagcc gctccgggtc gaactgcgcc ttcatccgga
     4981 ccatgttccg ctcggccagg gccaggtccg ccgcggcttc ctcgtcggtg tagcggtcaa
     5041 gctcgccgta gcgccgcttg atgccgagtc cggtcagggt ctccgggctt cgctccgccg
     5101 cttcgtcgaa cgccgagtcg aggaactcga ggagccgcgc gtcctcggcg gcggggccgg
     5161 ccgtcggggc actgccgccg tcggccgccg catcgggttg cgccgcttcc gccgcggccg
     5221 ggagcgcgag gtcctcgccc ttcgtggaat tcgtggcgca gccggcgagg agcagtgcga
     5281 tcaagggaag gatgggcttc acgatggcgg cctttcgggc tgatgcgcgc tgtttagcgc
     5341 ctcggcgcgg cgcgtccagc ctcgccggcc cagcggcgtg cgagcggctg caggtcgcga
     5401 taggcggccc agatcgaggc ggcccatttc cggaccgcct cgcgccacgc cgccggatcg
     5461 ccgatctcgg caaggtcgcc cagcgtcatg gctccgcgct cggccggcaa ggcgggcttg
     5521 tcgagcgccc ggtcgccgtc gagccagttc ctcaggccgg gggagccggt ggccgggctc
     5581 gcgtcctgct cgacgatctg cgccagcccg accagatggg cggcgagcga cttggcggaa
     5641 gcgcaataat cgtcgggatg ctgcaggcag tagcagtcca cgaacaaccg gtggacgccg
     5701 aagaagcggg cgtcgctgaa atcgcgggcg agcagcgcct cgaacgccgc tcggcagccg
     5761 gcggtgcccc cttcgacggc gcggccgcag ccgccgcagg cgtcgttcgt catcgccgct
     5821 cccccgccag ccagcacgga tcccattgcg gttcgggtcc gaagcgggcg gcgaggaagt
     5881 cgaccagcgc ccgcacccgc gccgtcgtcg cccggcccgg cggcatcacc gcgtggagcc
     5941 cgccttcctc gagcggatag tcgcgcagca gcggctcgag cgtgcctcgc tcgaaggccg
     6001 gggcggcgat gaagctgggc aggatgcaca cgcccagccc cgcctcggcg gcggcccgga
     6061 gcatctcgcc attgtcggcc ctcagccgcg gccggccgcg gacatattcc catttgcccc
     6121 cgacccggaa gcgccactgc tcgttggcgt agagcaggat gtcgtgcccg gcgaggtcgc
     6181 gcggatgctc cgggcgcccc cgcgcctcca gataggcggg gcttgccgtc ggcaccttgc
     6241 gcaccggcgc gatccggcgc gcgaccagcg ccgaatcctt gagcgatccg atccgaaccg
     6301 ccaggtcgaa gccgcccgcg gcgaggtcga cggggcggtc gtcgaaggag atgtcgagct
     6361 cgacccgggg attggcctcg gcgaactcgg ccagggccgg cgccagatgc ccgatcccga
     6421 acgaaagcgg ggcggaaagc cggatcggcc cggccacctg ggtgaccgcc tcggcgacca
     6481 cctcctcggc cgcctccagc tcggcgagga tattcgccgc gcgctcgaaa taggcctggc
     6541 cgacttcggt gggctgcgcc ccctgcgccg tccgcgtcag cagccgcgcc cccaattgcg
     6601 cctcgagccg ggcgacgcgg cgcgacagga tcgatttgga catgcccatc cgctcggcgg
     6661 cccggctgaa cgcgcccgtc tccgccaccc gcacgaaggc gagaacgtcg agcagatcga
     6721 ccgatgagcg ttgcatggcg gagcctttca ctttggtcca ttatggagtc gtcgccgctt
     6781 tgcgggaagc gccaaccggt ccagcctgcg cggcttccga tgtcgccggt gcgcggggcg
     6841 ccggcgggaa ctggggggat cgtcgatgac gaacaagctc aaccgaccgc tgctggccgc
     6901 cgtcctgccc gcggcgatgc tgatcccggc cgccccggcg gcggcccagg gctcgcccac
     6961 ctacgaatat gcggccaagt tcgtctgcgc cgccagctcc aacctcactc cgccggtggc
     7021 cgtcgggcac tattatacgg ccgtcaacgt ccacaatccg ggcattgacg tgaaagtggc
     7081 ctggaaggtc gcgctcgccg gcacgatcct gcccggcaag atcaccggct tcgacagcca
     7141 gctgctcaag aacgacgagg ccatggagat cgactgccgg tcgatccgca accggatcac
     7201 ggccagcggc cttcccaatc cggccgcctt cttcaccggc ttcgtcgtga tccgggcgcc
     7261 ggcgccggtg gacgtggtga gcgtctacac cgcgtccgcg caggtgaacg gcccggtcgt
     7321 gtcgatccat accgagcgcg tccctgcccg gcagctgcct tagcgcgttg cggtttcgac
     7381 aacactaaag tgcattagag cgctctaccc gcaacgctgc gtcgggctta tctcccctcc
     7441 acaagctcag tggaggaaga catgaccaac gtcctcatca tcgacagcgc cgccaccggc
     7501 gacgcctccg tctcgcgccg gctgaccggc gagctcgagg cgatcctgcg cggccgcggc
     7561 gcgggccgca tcgtccgccg cgacgtcggc accgagacca tcccgcatct caccgcggcg
     7621 accacggcgg cgatccgcgg cgccccggcc gaaaccgacg ccgctcgcga ggccatcgcc
     7681 ctgtccgaca cgctgatcgc cgagctcaag gccgccgacc tcgtcgtgat cggagcgccg
     7741 atgtacaatt tcggcatgcc ttcgaccctc aaggcctggt tcgaccacgt cctgcgcgcc
     7801 ggcatcacct tccgctactc gcaaaccggg cccgagggcc tggtcaaggg caagaaggcg
     7861 atcgtcatcg agagccgggc gggcctctac agcgagggcc cggccgcggc gatggacagc
     7921 caggagccgc acctgcgcac cctgctcggc ttcatgggcc tcgacgacgt cgcgttcgtc
     7981 cgggtcgaga agctcgcctt cggccccgac caggaggcgg cctcgttcgc cgaggcgatc
     8041 gaggagctcc gttcgctcgc cgccgacgac ttgccgctgg cggcctagcg ccgccgcgcc
     8101 tccgcccgcg ccgccgcccc ctcctggcgg cgggcggacc ggtcaccata ttgacgttcc
     8161 aggagacgag agatgaagct ctactacgct cccggcgcct gctcgcaggc cccccacatc
     8221 gtcgcccgcg aggccggcct cgacatcgag ctcgccaacg tgcgcattcc cgacaaggtc
     8281 accgacgacg gcgagaagct gaccgacgtc aatccgaagg gcgccgttcc gaccctccgg
     8341 ctggacgacg gtgaggtgct caccgagaat gcggtcatcc tccagtatct cgccgatcgg
     8401 gccgagggct cggagctggc gctgccggcc tccgggatcg agcgctaccg cctgctcgaa
     8461 tggctgaact tcatcgccac cgagcttcac aagggcttcg gcccgctgtg gaatccggcc
     8521 acgcccgact cgttcaagga ggcgacccgc gaggcgctcg gcaagaagtt cgactatctc
     8581 caggagaaga tcggcgacgg tccctacatc ctgggcgagc gcttttcgat cctcgacgcc
     8641 tatgccttcg cggtgctcaa ctggtcggcg atccactcga tcgacatcgg ccgctggccc
     8701 gggctcgccg cctatctcca gcgcgtcgcc gcccgcccgg cggtgcagga gacgctgcgc
     8761 gccgaagggc tgatcgaata gtccgtcatc ccgacgggcg accaaccccg ccggccgccg
     8821 cgccctgttt caccctgaga tcccggcgtt cgccgggacg acgaggaccg aatgatcgac
     8881 atccgcccct tttccaccct cggccacgcc gatcatggct ggctcaacgc ccgtcaccat
     8941 ttctccttcg cggactatca cgcgccggcc cgcatgggct ggggcgcgat ccgggtgtgg
     9001 aacgacgacg agatcgccgc gcgctccggc ttcccgccgc acccgcaccg ggacatggag
     9061 atcatcacct atgtccgcga gggggcgatc acccatcagg attcgatggg caacaagggc
     9121 cgcaccgcgg ccggcgacgt ccaggtgatg tccgcgggca ccggcgtccg gcacgccgaa
     9181 tataatctcg aggacgagac gacgaccctg ttccagatct gggtgcagac cgacaagccg
     9241 ggcgcggagc cggcctgggg cgcgaggcaa ttccctaggg acggccgcgc cggcaatttc
     9301 gtcacgctgg cgagcggcta tgcggaggac ggggacgccc tgaagatcaa cgccgcggcc
     9361 cgggtgctcg gcgcgaccct gaattcaggc gagacggccg agctcgagct cgacccggcc
     9421 cgccacgtct atctcgtcgc cgtcggcgga gcgatcgagg tcaacggctg gcgcgccgag
     9481 ccccgcgacg gcatcgcggt caccggcgag gagaagctgg cgatccgcgc gatggaaaac
     9541 gccgagatcg tcctcgtcga cgccaaatga tttcctcccc cgactagaat atcttgcgaa
     9601 ggctgaagct cagggaccgg cccagcggat cgagaaatgc cggctgatag ctgagcggga
     9661 tcgagcccgc ttcgtcccgc acttccggcc gccggttgaa cagattgttg attccgagac
     9721 tgacccgagt ccgcttcagc cagcctgggg ccgtctcgcc tccaaaatgg tctgcaaggt
     9781 tcgcgaacag attgatggtc acggtcgcat ggtccgagaa gatcagatct tccgagctgc
     9841 cggccaggcc tgcggggagg ccgctcacgg tggttccgct ctgccaattg actttgaccc
     9901 gggcgccgag gccgcgcttg aaggcggttg cctggaactc gagctcgtga cggggcctg
//
LOCUS       DASVQZ010000312         7357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_260806, whole genome shotgun sequence.
ACCESSION   DASVQZ010000312 DASVQZ010000000
VERSION     DASVQZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260806"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF547_08615"
     CDS             <1..558
                     /locus_tag="VF547_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEX8262921.1"
                     /translation="VDLLATRPPTFARNVFLKGNHEEFFLGVLGGDDSGVQHWLTYGG
                     TECAQSYGLGSGWMLNATPAAIMERLIEQVPESHVRFLGEMADSFRFGDYLFVHAGIR
                     PGVALDRQTSRDLRWIREGFLDDLTDHGVVVVHGHTIVERPEQHPNRIAIDTGAYRYG
                     TLTALGIEGTDRWFIEARASESEAA"
     gene            790..1788
                     /locus_tag="VF547_08620"
     CDS             790..1788
                     /locus_tag="VF547_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX8262922.1"
                     /translation="MTILVTGAAGFIGHHVAARLLGRGEQVLGIDNVNDYYSVKLKRD
                     RIAEQRSRHDNAFSFVEVDFADKAALDSALEGREFDRIVHLGAQAGVRYSIENPHAYV
                     HSNLVGHLNLLEVARQRGSSHLVYASSSSVYGGNESLPFRVEDRVDHPLSLYAATKKA
                     DELISETYAHLYRLPQTGLRFFTVYGPWGRPDMAMWLFTKAILAGETIDVFGEGNMRR
                     DFTYIDDIVSGVVACLDNPPPDDGEEKAGGSRSPHRVYNIGNNRSEELTRMIGLIERA
                     CGREADKRLLPMQPGDVRDTYADISAIQRDLGFQPTTSIDEGVPRFVEWYRGYHGL"
     gene            complement(1826..3097)
                     /locus_tag="VF547_08625"
     CDS             complement(1826..3097)
                     /locus_tag="VF547_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8262923.1"
                     /translation="MPSSEQIVVVGLGYVGLPLAVALSRSFAVTGLDIDERRIEELKL
                     GVDRTREIEPDALKASPLRLTSRAVDAAGADVYIVTVPTPVDERNRPDLSPVIGATRS
                     VATLLDPSKKPIIVYESTVYPGVTEDICGPLIEEVSGLQRGRDFFLGYSPERINPGDR
                     EHTVDRIVKVVAGENQAVADRLAAIYGAVTTGGVFRAASIKTAEAAKVIENAQRDINI
                     AFMNEITQIFARLDLSIWDVLAAARTKWNFLPFQPGLVGGHCIGIDPYYLSHRAQELG
                     HHPEVILAGRSINDGMGRWVADLLHERRGERPGSALVMGLTFKEDVPDLRNSKVIDVV
                     RRLAELGHEVTVHDPLGDAAEARHEYGVEPAPDALDGRYDLVLVAVPHAAYGELDDSK
                     VAGLVAEGGLLADLKNLYAGRALPGVERWTL"
     gene            complement(3187..3786)
                     /locus_tag="VF547_08630"
     CDS             complement(3187..3786)
                     /locus_tag="VF547_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262924.1"
                     /translation="MTSALRLPRAIALLAAALLAFAGAPALAAVSITFYSKELGASFP
                     HAFVIVEGTPDRGGPRIEEDYGFTAKTISPAILWGKVAGEVVTDHSPGYVRGSDKHFT
                     LALTDAEYDRAMATVARWRSARQPSYDLDRHNCVHFVAEIAAAIGLAADTRRFMKKPR
                     SYLEALTEANRARLQARSATFHRLAPAVRGKPGRGKPGA"
     gene            4076..4330
                     /locus_tag="VF547_08635"
     CDS             4076..4330
                     /locus_tag="VF547_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262925.1"
                     /translation="MGFKGLIAATTSVALIAAPTMAAAQTSAAAARSTEVAPATETVE
                     GEQLRGGWIIPLIALIAIILGLCVATDQCGGDDDDLPHSP"
     gene            4422..5426
                     /locus_tag="VF547_08640"
     CDS             4422..5426
                     /locus_tag="VF547_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8262926.1"
                     /translation="MRERPGLSRRALRRLGFAPLAALLLSAQAPPGPPPSPLPAAELA
                     GSTTLRQDDLRVALVAYRLALAGSALCPERYPLTGMLFHHLAEYEPRDRPLMIRRYGL
                     DRGPGVLTVIAGTPAARAGLVAGDVLIAVNGRPLPTGSSVLRETKREKWRRLADEAEG
                     ELETALRQGPAELRVLREGREILLRLGSIPACLGRVRLARSTQVNAFATGRTVVMTLA
                     MLSFLRNDDELAVVLGHELAHNIFNHRAMRNEEGILRGLGIKPSAVWKREEAADRFGL
                     RLMAAAGYDLDSAIPFWRRYLGKYDWFPQIFRSHPSLGARERIAAEEIAAIRRERAGL
                     "
     gene            complement(5455..6255)
                     /locus_tag="VF547_08645"
     CDS             complement(5455..6255)
                     /locus_tag="VF547_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX8262927.1"
                     /translation="MTDTPASMTYGGYLALDRILSAQHPISDHHDEMLFVVIHQTKEL
                     WLKQIIHELKLSRDMIRRDRLIEVHKALSRVSRIQAVMTLSWDVLATMTPSDYTSFRA
                     VLGSSSGFQSAQFREFEYRLGLKDGAHLKFQEEGSGPYGCLLAALHEPSLWDEANAAA
                     ARSGLPVPADRLERDWSEPYQPSAGVEAAWAEVYREPERLWPLYQLAEKLVDIDDALA
                     VWRHKHVVTVERIIGLKRGTGGTAGVAYLQSTLAKRAFPELWSLRTEV"
     gene            complement(6258..6737)
                     /locus_tag="VF547_08650"
     CDS             complement(6258..6737)
                     /locus_tag="VF547_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX8262928.1"
                     /translation="MSRTMIDRRTMIGLLGGVAAAPFLSGAAGAAAPGGYVMWRDPGC
                     GCCLEWAKRVEAAFGRKLPVVDAPNMAAVKKAQGVPAALRSCHTALIQGVVVEGHVPP
                     EDIKRLVASRRKGVKGLAVPGMPAGSPGMDVGHNHRQRYQVFAFDAGGRSSLFATHG"
     gene            complement(6734..>7357)
                     /locus_tag="VF547_08655"
     CDS             complement(6734..>7357)
                     /locus_tag="VF547_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8262929.1"
                     /translation="EIVQRLPQPLGWEAIAEALKRLNFHDDAALMEAIALKRVSDVAV
                     MEALIPGSTGKEGVKPPPQRTAISIKGLTPGVAFQLAECCHPIPGDRIVGLRREGTEI
                     EVHVIGCDSLADGVDADWLDLAWGDESDGGTARLCVVIKNEPGSLAVIAGILGRHGAN
                     IVSMDQASRDGSFHTFHLDIEVHDVQHLMRILAALRAADVVSSAERL"
BASE COUNT         1141 a         2557 c         2500 g         1159 t
ORIGIN      
        1 gtcgacctgc tcgccacccg ccccccgacc tttgcccgca atgtcttcct gaagggcaat
       61 cacgaggaat tcttcctcgg cgtcctcggc ggcgacgaca gcggggtcca gcactggctc
      121 acctatggcg gcaccgaatg cgcccagagc tacgggctcg gcagcggctg gatgctgaac
      181 gccactccgg ccgcgatcat ggaacggctg atcgagcagg tgcccgagag ccatgtccgc
      241 ttcctcggcg agatggccga cagcttccgc ttcggcgatt atctgttcgt ccacgccgga
      301 atacgccccg gggtcgcgct cgaccgccag acctcccggg acctgagatg gatccgcgag
      361 ggctttctcg acgacctcac cgaccatggg gtggtcgtcg tccacggcca caccatcgtc
      421 gagcggcccg agcagcatcc gaaccggatc gccatcgaca cgggagccta tcgctacggc
      481 accctgaccg ctctcggcat cgaaggcacc gaccgctggt tcatcgaggc gcgcgccagc
      541 gagagcgaag ccgcctgacg gcccggcctt gatttcgcgg agccgccggc tagagcggtg
      601 aaccacgaga ttggatcgta cccgccgttc gccctgagcc tgtcgaaggg ctttccttgc
      661 cgcttatcgc tgcaggaaag ggcttcgaca ggctcagccc gaacgggata ggacgatcac
      721 gccgcccaca ctcttgtgcc ggacgcccgt caccgggacc ggagccatcg aacaggaaaa
      781 taaagcgaaa tgaccatcct cgtgaccggc gcggccggct tcatcggcca tcacgtcgcg
      841 gcgcggctgc tcggccgggg cgagcaggtg ctcggaatcg acaacgtcaa cgattattac
      901 agcgtcaagc tgaagcgcga ccggatcgcc gagcagcgct cgaggcacga caatgcgttc
      961 agcttcgtcg aggtcgactt cgccgacaag gcggcgctcg actccgcgct cgaggggcgc
     1021 gagttcgacc gcatcgtcca tctcggcgcc caggcgggcg ttcgctactc gatcgagaat
     1081 ccgcacgcct acgtccattc gaacctggtc ggccatctca acctcctcga ggtggcccgg
     1141 cagcgcggct cctcgcacct cgtctacgct tcctcctcct cggtctacgg cggcaacgag
     1201 agcctgccct tccgggtcga ggaccgggtc gatcacccgc tctcgctcta cgcggccacc
     1261 aagaaggccg acgagctgat cagcgagacc tatgcccatc tctatcgcct tccccagacc
     1321 ggcctgcgct tcttcaccgt ctatggcccc tggggccgcc cggacatggc gatgtggctg
     1381 ttcaccaagg cgatcctcgc cggcgagacg atcgacgtct tcggcgaggg caacatgcgc
     1441 cgcgacttca cctatatcga cgacatcgtc agcggggtcg tcgcctgcct cgacaacccg
     1501 ccgccggacg acggcgagga gaaggccgga ggaagccgct ccccgcaccg ggtctacaac
     1561 atcggcaaca accgctccga ggagctcacc cggatgatcg ggctgatcga gcgcgcctgc
     1621 ggccgcgagg cggacaagcg cctgctcccc atgcagcccg gcgacgtccg cgacacctat
     1681 gccgacatca gcgccatcca gcgcgacttg ggcttccagc cgaccaccag catcgacgaa
     1741 ggcgtgccgc gcttcgtcga atggtaccgc gggtatcacg ggctctagcc gcgaccttcc
     1801 ggccttccgc gccgaatagg gcggctcaga gggtccagcg ctccacgccg ggcagcgctc
     1861 ggccggcata gaggttcttg aggtcggcga ggaggccgcc ttcggcgacc aggccggcga
     1921 ccttcgaatc gtcgagctca ccataggcgg cgtgcgggac cgcgacgagg acgaggtcgt
     1981 agcgcccgtc gagcgcgtcc ggggccggct cgaccccata ttcgtgcctc gcctcggcgg
     2041 cgtcgccgag cgggtcgtgg acggtgacct cgtggccgag ctcggcgagt cggcggacga
     2101 cgtcgatcac cttggagttg cgaaggtcgg ggacgtcctc cttgaaggtg agccccatca
     2161 ccagcgccga gcccgggcgc tcgccgcggc gctcgtgcag gaggtccgcc acccagcggc
     2221 ccatgccgtc gttgatcgag cggccggcga ggatcacctc cggatgatgg ccgagctcct
     2281 gggcgcggtg gctcaggtaa taggggtcga tgccgatgca gtggccgccg accaggccgg
     2341 gctggaaggg gaggaagttc cacttggtgc gggcggcggc gagcacgtcc cagatcgaca
     2401 ggtcgagccg cgcgaagatc tgggtgatct cgttcatgaa ggcgatgttg atgtcgcgct
     2461 gggcgttctc gatgaccttg gcggcctcgg cggtcttgat cgaggccgcc cggaagacgc
     2521 cgccggtggt gaccgcgccg tagatcgccg cgagccggtc ggcgaccgcc tggttctcgc
     2581 cggcgacgac cttgacgatg cggtcgaccg tatgctcgcg gtcgcccgga ttgatccgct
     2641 cgggcgagta gccgaggaag aagtcgcggc cccgctggag cccggacacc tcctcgatca
     2701 gcggcccgca gatgtcctcg gtcactccgg gatagacggt gctctcgtag acgatgatcg
     2761 gcttcttcga cgggtcgagc agggtcgcga ccgagcgcgt cgctccgatc accgggctca
     2821 agtccggccg gttgcgctcg tcgaccgggg tcgggacggt gacgatgtag acgtcggccc
     2881 cggcggcgtc gacggcgcgg ctggtcagcc gcagcggcga ggccttgagc gcgtccggct
     2941 cgatctctcg ggtccggtcg actccgagct tgagctcctc gatccggcgc tcgtcgatgt
     3001 cgagcccggt gacggcgaag ctccggctca gggcgaccgc gagcggcagc ccgacatagc
     3061 ccaggccgac gacgacgatc tgctcggagg aaggcacttg gatcagcttc gcttttcagg
     3121 aggtcgggaa ggctcggcac ggcggccggc cttgcgcggc gctaacatcc gacgcggccg
     3181 caaatgtcaa gcgcccggct tgccccggcc cggctttccc cgcaccgccg gagccagccg
     3241 gtgaaaggtc gcgctccgcg cctggagccg cgcccggttc gcttcggtca gggcctcgag
     3301 gtaggagcgg ggcttcttca tgaagcgccg ggtgtcggcg gccagcccga tcgccgccgc
     3361 gatctcggcg acgaaatgga cgcaattgtg cctgtcgaga tcgtaggagg gctgccgggc
     3421 ggagcgccag cgcgcgaccg tcgccatcgc ccggtcgtat tcggcgtcgg tcagcgccag
     3481 ggtgaaatgc ttgtcgctgc ccctgacgta gccgggcgag tggtcggtga cgacctcgcc
     3541 ggcgaccttc ccccacaata tcgccgggct gatcgtcttc gcggtgaagc cgtaatcctc
     3601 ctcgattcgc gggccgccgc ggtcgggcgt gccctcgacg atcacgaagg cgtgggggaa
     3661 gctggcgccg agctccttcg aatagaaggt gatcgagacc gcggccagcg ccggcgcgcc
     3721 ggcgaaggcg agcagcgcgg ccgccagcag ggcgatcgct cgaggcaggc gtaaggctga
     3781 cgtcaccggc tcctccccag gtcgggcttc gcgaaccccc tgaaacaaaa agccaattcg
     3841 ggcaatcccc ctcagagtgt cgcttccgtg caacagccgg cccaattcgt gcgccccgat
     3901 gaaccaaact cggccggttt cgttcgagtg aggcttcgta aacttctctg gggttacggg
     3961 gtggatcggg atttccgacg cgcggccatt tcagggtaga cagccccgga acggtcgatt
     4021 aggtagggcc ccgaaatcga gaagatcaga ctcgagtacg aagggagaag ctgacgtggg
     4081 tttcaaggga cttattgctg cgaccacatc ggttgcgctg attgcggcgc cgaccatggc
     4141 ggcggcgcag acttccgcgg cggccgcccg ttccaccgag gtggcgccgg cgactgagac
     4201 ggtcgagggt gagcagctgc gcggcggctg gatcattccg ctgatcgccc tcatcgcgat
     4261 catccttggc ctgtgcgtcg ccactgacca gtgcggcggc gacgacgacg acctgccgca
     4321 cagcccgtaa ccgggccggc tggtacgtca gccattttga gtttctggcc ggatgaagag
     4381 cttcttcatc cggccatttc ttgtgcgtga gaggattggc tgtgcgtgaa aggccgggcc
     4441 tgagccgccg cgccctgcgc cgcctcggct tcgccccgct cgcggcgctc ctcctgtcgg
     4501 cgcaggcgcc gccggggccg ccgccctcgc cgctcccggc cgcggagctg gcgggctcga
     4561 ccacgcttcg ccaggacgat cttcgcgtcg cgctcgtcgc ctatcggctg gcgctcgcgg
     4621 gctccgccct ctgtccggaa cgctatccgc tgaccgggat gctgttccac cacctcgccg
     4681 aatacgagcc gcgcgaccgc ccgctgatga tccgccgcta cggactggac cgcggtccgg
     4741 gcgtcctcac cgtcatcgcc ggcactcccg ccgcccgcgc cggcctcgtc gccggcgacg
     4801 tcctcatcgc cgtgaacggc cggccgctgc cgaccggaag ctcggtcctt cgcgagacga
     4861 agcgcgagaa atggcgccgc ctcgccgacg aggccgaagg cgagctggag acggcgctgc
     4921 gccagggccc ggccgagctt cgcgtgcttc gcgaggggcg cgagatcctc ctgcgcctgg
     4981 gctcgattcc cgcctgcctg ggccgggtcc ggctcgcccg ctcgacccag gtcaacgcct
     5041 tcgccaccgg ccgcaccgtc gtcatgaccc tcgccatgct gagcttcctc aggaacgacg
     5101 acgagctcgc cgtggtcctc ggccacgagc tggcgcacaa catcttcaac caccgcgcga
     5161 tgcgcaacga ggagggcatc ctgcgcggcc tcggaatcaa gcccagcgcg gtctggaagc
     5221 gcgaggaagc cgccgaccgc ttcgggctga ggctgatggc cgccgccggc tacgatctcg
     5281 attcggccat ccccttctgg cggcgctatc tcggcaaata tgattggttt ccgcagatct
     5341 tccgaagcca cccgagcctc ggcgcgcgcg agcggatcgc ggccgaggag atcgccgcga
     5401 tccggcggga aagagcgggc ctttgaggac tctcccggcg aggccgcggg atttctagac
     5461 ctcggtcctc agcgaccaca gctcggggaa ggcgcgcttg gcgagcgtcg actgcagata
     5521 ggcgaccccc gccgtcccgc cggtgccgcg cttcagcccg atgatccgct cgacggtgac
     5581 gacatgcttg tgccgccaga cggcgagggc atcgtcgatg tcgaccagct tctcggcgag
     5641 ctggtacaga ggccaaagcc gctccggctc gcgatagacc tcggcccagg ccgcctcgac
     5701 cccggcgctc ggctggtagg gctcgctcca gtcgcgctcc agcctgtcgg cgggcaccgg
     5761 caggcccgag cgcgcggccg cggcattggc ctcgtcccac aggctcggct cgtgcagcgc
     5821 cgcgagcagg cagccatagg gcccggaccc ctcctcctgg aacttgagat gggcgccgtc
     5881 cttgaggccg agccggtatt cgaactcgcg gaactgcgcc gactggaagc cggagctgga
     5941 gccgagcacg gctcggaagc tggtgtagtc ggaaggggtc atcgtcgcga gcacgtccca
     6001 gctcaaggtc atcaccgcct ggatccgcga cacgcgcgac agcgccttgt ggacctcgat
     6061 cagccggtcg cgccggatca tgtcgcgcga cagcttcaat tcgtggatga tctgcttgag
     6121 ccacaattcc ttggtctggt ggatgaccac gaacagcatc tcgtcatgat ggtcggagat
     6181 cggatgctgg gcggagagga tccggtcgag cgcgagatag ccgccatagg tcatcgaggc
     6241 gggcgtgtcg gtcatcctca gccgtgcgtg gcgaacagcg aggaccgccc gcctgcgtcg
     6301 aaggcgaaaa cctggtagcg ctgccggtgg ttgtgaccga cgtccatgcc cggcgatccc
     6361 gccggcatgc cgggtacggc gaggcccttg acgcccttgc gcctcgacgc gaccagccgc
     6421 ttgatgtcct cgggagggac atggccctcg acgacgacgc cctggatgag ggcggtgtgg
     6481 caggaccgca gcgccgcggg gacgccctgg gccttcttca cggcagccat gttgggcgcg
     6541 tcgaccaccg gcagcttcct gccgaacgcc gcttccaccc gtttcgccca ttcgaggcag
     6601 cagccgcagc cgggatcgcg ccacatgacg tagccgcccg gcgccgccgc tccggccgct
     6661 ccggagagga agggtgccgc cgcgaccccg ccgaggaggc cgatcatcgt ccggcggtcg
     6721 atcatggtcc tgctcataga cgctccgcgc tggacacgac gtcggcggcc cgaagcgccg
     6781 ccaggatgcg catcagatgc tgcacgtcgt gcacctcgat gtcgaggtgg aaggtgtgga
     6841 agctgccgtc gcggctggcc tggtccatcg agacgatgtt ggcgccgtgg cggccgagga
     6901 tgccggcgat caccgcgagg gagcccggct cgttcttgat gacgacgcac aggcgagcgg
     6961 tgccgccgtc cgattcgtcg ccccaggcga ggtcgagcca gtcggcgtcg actccgtcgg
     7021 caaggctgtc gcagccgatg acgtggacct cgatctcggt gccctcgcgc ctcaggccga
     7081 cgatcctgtc gccggggatc gggtggcagc attcggcgag ctggaaggcg accccggggg
     7141 tcagcccctt gatcgagatg gcggtccgct gcggcggcgg cttcacacct tccttgccgg
     7201 tcgagccggg gatcagcgcc tccatcaccg cgacgtcgct gacccgcttg agagcgatcg
     7261 cctccatcaa agcggcgtcg tcgtggaaat tgagtcgctt caaagcctcc gcgatcgctt
     7321 cccagccgag cggctggggc aggcgctgga cgatctc
//
LOCUS       DASVQZ010000313         6006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_740427, whole genome shotgun sequence.
ACCESSION   DASVQZ010000313 DASVQZ010000000
VERSION     DASVQZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740427"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VF547_08660"
     CDS             complement(<1..1017)
                     /locus_tag="VF547_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8262930.1"
                     /translation="MAGAAAILLTDAAYCQETPPEPEPAPAAESAAASEAAARADPHA
                     DHTQEIIITGFSRNREDVLSGTSVVSGAELARDLRPTIGETLSRQPGVSATSFGPNAS
                     RPVLRGFQGERVRVLTDGIGSLDASNTSVDHAVAINPLTADRIEVLRGPSALLFGSAA
                     IGGVVNVIDARIPRRVPDEGAHVAALLTYGSAADERSANATIDVPVSGKFVLHADGNY
                     SRSDDLRIGGFVLARDLRAEAAASPDPEIRALAELRDRLPNTAAETSDVALGLGYVSG
                     DTNFGISVNRYDSRYGVPVRYSLEPGVEAEAPRIDIRQNRADARAEIEAGSGLVESVR
                     FRAGY"
     gene            1172..1951
                     /gene="paaG"
                     /locus_tag="VF547_08665"
     CDS             1172..1951
                     /gene="paaG"
                     /locus_tag="VF547_08665"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEX8262931.1"
                     /translation="MTYRTIDFSSEGGIARLTLNRPDRLNSFTVEMHEEVADALGKLD
                     GARVLVLTGAGRGFCAGQDLNDRAVAPGEAVDLGESVEHRYNPLIRTLVGLPFPVIAR
                     VNGVAAGAGANIALACDIVVAAKSARFIQSFASIGLIPDSGGTWVLPRLVGQARALGL
                     ALTAEPLAAEQAAQWGLIWKAVEDEALDAEVDALAERFAAAPTRGLARIKKMIRESWG
                     HGLDEELDLQRDAMRELGFSEDYREGVAAFMEKRKPNFTGR"
     gene            2058..4076
                     /gene="paaZ"
                     /locus_tag="VF547_08670"
     CDS             2058..4076
                     /gene="paaZ"
                     /locus_tag="VF547_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEX8262932.1"
                     /translation="MKTLKLLNYAQDRWYGAEGGLAPVESAVTGEVVAETGSGGLDFA
                     AMLDHARKVGGPALRRLTFHERAHMLKALAGAIMARKEELYELSYETGATRTDGWIDI
                     EGGAGTLFSFSSKGRRELPNSRILVDGALEPLSKGGSFVGQHVFTPLQGVAVHINAFN
                     FPVWGMLEKLGPTLLAGVPAIVKPASATGYLAEAAFRIMIEAEVLPAGALQLVMGGVG
                     DLFDHLTLQDVVSFTGSAQTAMKLQSHPVIARESVRFIAERDSLNASVLGPDAGPGTA
                     EFDLFVKEVAREMTVKAGQKCTAIRRAMAPAEHLDAVQAALIERLGKARIGNPREEGV
                     TMGALASRAQLRSVREAVGMLAKVARIVHGDPEKSPVSGGGAFISPILLRTDSPWSVA
                     EVHDIEAFGPVSTIMPYRDLGDAIGLANRGMGSLALSLFTFDPSVAEEFVLGAGAYHG
                     RMLILDRTSAKESTGHGSPLPVLVHGGPGRAGGGEEMGGIRGVTHYMQRTALQGSPAV
                     LSSVVGQWLPGAPKPEGDRHPFRKRIAELDVGYTLKTASRTVSLDDIEHFAAFTGDNF
                     YAHMDEAAAKANPIFDGRVAHGYLILSFAAGLFVDPDPGPVLANYGLENLRFQTPLYP
                     GDSMRVELTVKSKSVRNAEMGEVRWAVYVFNQKDEVVASYDLLTMNTP"
     gene            4073..4846
                     /locus_tag="VF547_08675"
     CDS             4073..4846
                     /locus_tag="VF547_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX8262933.1"
                     /translation="MIDRTAPLLLFALAACGASATAEPPVDGYRIVREYPHDPTAFTQ
                     GLLYLDGRLYESTGLNRQSTIREVRLEDGKVLRSVSIAPQYFGEGLVNWGPDLISLTW
                     QDGIGFRWNRADFRPKGQFRYPGEGWGLTQDGKSIIMSDGTSQLRFLDPATMRERRRL
                     AVTYQGQPVERLNELEWVKGEIFANIWMGRRIARIDPKSGVVRGWIDLSELAAENANG
                     DPDSVLNGIAYDARRDRLFVTGKNWAKLFEIDIVPKPKP"
     gene            4950..5381
                     /locus_tag="VF547_08680"
     CDS             4950..5381
                     /locus_tag="VF547_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8262934.1"
                     /translation="MKIAECMTAEVETVSPDQPIREAAQFMLRADAGAMPVGEGDKLV
                     GMVTDRDIAVRGVAAGRGPDTPVREVMTEEVIYCFEDDEVEEVALKMSDRQVRRMPVL
                     SRENETLVGIVSLGDISRSEQSEAASVALDGITDPGGEHSQ"
     gene            complement(5498..>6006)
                     /locus_tag="VF547_08685"
     CDS             complement(5498..>6006)
                     /locus_tag="VF547_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8262935.1"
                     /translation="GGKTTLVTDPQHKMRRDREITDEALCILLRDRVRARLVPMIKRA
                     FQFEATRMERYIVGSYEAGAGHFRPHRDNTTKGTAHRRFAVTINLNAGDYEGGDLRFP
                     EFGARTYRAPTGGAIVFSCSLLHEATPVTKGTRYAFLPFLYDDAAAKQREANNPHLDE
                     GIGAYKAG"
BASE COUNT          926 a         2102 c         2097 g          881 t
ORIGIN      
        1 atagccggcg cggaagcgaa cgctctcgac caggccggag ccggcctcga tctcggcccg
       61 ggcgtcggcc cggttctggc ggatgtcgat gcgcggcgcc tcggcctcga ctcccggctc
      121 gagggaatag cgcaccggca cgccgtagcg gctgtcgtag cggttcaccg agatcccgaa
      181 attggtgtcg ccgctcacat agccgaggcc gagcgcgacg tcggaggtct cggcggcggt
      241 gttgggcaga cggtcccgaa gctcggccag cgcgcggatc tcggggtccg ggctcgccgc
      301 cgcttcggcg cgaaggtccc gcgccaggac gaagccgccg atccgaaggt cgtcgctcct
      361 gctgtaattg ccgtcggcgt ggagcacgaa cttgccggat accggcacgt cgatcgtcgc
      421 gttcgccgag cgctcgtccg cggccgagcc gtaggtgagg agggcggcga catgcgcccc
      481 ttcgtccggc acccggcgcg ggatccgcgc atcgatgacg ttgaccacgc ctccgatcgc
      541 cgccgagccg aacagcaggg ccgaagggcc tcgaagcact tcgatccggt cggcggtcag
      601 cgggttgatg gcgacggcgt ggtcgacgct ggtgttggac gcatcgaggc tgccgatgcc
      661 gtcggtcagc acccgcaccc gctcgccctg gaagccgcga agcaccggcc tcgaggcgtt
      721 gggcccgaac gacgtcgccg agactcccgg ctggcgcgac agggtttcgc cgatcgtcgg
      781 ccggaggtcc cgcgccagct cggccccgga gaccacggag gttccggaca gcacgtcctc
      841 gcggttgcgg ctgaagccgg tgatgatgat ttcctgggta tggtccgcat gcgggtcggc
      901 ccgcgccgcg gcctcgctcg ccgcggcgga ctccgccgcc ggcgcgggct cgggctcggg
      961 cggcgtctcc tggcaatagg ccgcgtcggt aagcaggatg gccgcggcgc cggccatcag
     1021 tacgtgtctc agcaaaggta ggacctcccg gtgaggacgg cacataggta cttgtgacac
     1081 tataacaaac ccgctttgcc gcgcgattaa cacgcctgac ccgcgtccgc cccacttccg
     1141 cttgccggcc tgccggtcta gagcggcggc aatgacctac cggaccatcg acttctcgag
     1201 cgagggcggg atcgcgcgcc tgaccctgaa ccggccggac cggctcaaca gcttcacggt
     1261 cgagatgcac gaggaggtcg ccgacgcgct cggcaagctc gacggcgccc gggtgctggt
     1321 cctgaccggc gccggccgcg gcttctgcgc cgggcaggac ctgaacgacc gggcggtcgc
     1381 gccgggcgag gcggtcgacc tcggggagtc ggtggagcat cgctacaacc cgctgatccg
     1441 cacgctcgtc gggctcccct tccccgtcat cgcccgggtc aacggcgtcg cggcgggcgc
     1501 gggcgccaac atcgccctgg cctgcgatat cgtcgtcgcc gcgaaatcgg ccaggttcat
     1561 ccagtccttc gcctcgatcg gcctcatccc ggacagcggc ggcacctggg tcctgcccag
     1621 gctggtcggc caggcccgcg cgctcggcct cgccctcacc gccgagccgc tggcggccga
     1681 gcaggcggcg caatggggcc tgatctggaa ggcggtcgag gacgaggcgc tcgacgccga
     1741 ggtcgacgct ctcgccgagc gcttcgccgc cgcgccgacg cggggcctgg cccggatcaa
     1801 gaagatgatc cgcgaaagct ggggccacgg cctcgacgag gagctcgacc tccagcgcga
     1861 cgcgatgcgc gagctcggct tcagcgagga ctatcgcgag ggcgtcgccg ccttcatgga
     1921 gaagcgcaag ccgaatttca cgggcaggtg aggtccgtca ttgcgaggag cgaagcgacg
     1981 cggcaatcca gcccggcggt cgcgacgagc gcaggactgg gttgcttcgc gacgctcgca
     2041 atgacgggaa tggacggatg aagaccctga agctgctcaa ctacgcccag gaccgctggt
     2101 acggcgccga gggcgggctc gccccggtcg agagcgccgt caccggcgag gtggtcgccg
     2161 agacggggag cggcggcctc gacttcgcgg cgatgctcga tcatgcccgc aaggtcggcg
     2221 gcccggccct gaggcgcctc accttccacg agcgcgccca catgctgaag gcgctggcgg
     2281 gcgcgatcat ggcccgcaag gaggaattgt acgagctcag ctacgagacc ggcgccaccc
     2341 ggaccgacgg ctggatcgac atcgagggcg gcgccggcac gctcttctcc ttctcctcga
     2401 agggccggcg cgagctgccc aacagccgga tcctggtcga cggcgccctc gagcccttgt
     2461 ccaagggcgg aagcttcgtc gggcagcacg tcttcacccc gctccaggga gtcgcggtcc
     2521 acatcaacgc cttcaacttc ccggtctggg ggatgctgga gaagctcggc ccgaccctgc
     2581 tcgccggcgt gcccgcgatc gtcaagccgg cctcggcgac cggctatctg gccgaggccg
     2641 cgttccggat catgatcgag gcggaggtgc ttccggcggg cgcgctgcag ctggtgatgg
     2701 gcggggtcgg cgacctgttc gaccatctga ccctgcagga cgtggtcagc ttcaccggct
     2761 ccgcccagac cgcgatgaag ctccagtccc atcccgtgat cgctcgcgag agcgtccgct
     2821 tcatcgccga gcgcgacagc ctcaacgcct cggtgctggg gccggacgcg gggccgggga
     2881 ccgcggaatt cgacttgttc gtcaaggagg tggcgcggga gatgacggtc aaggcggggc
     2941 agaaatgcac cgccatccgc cgcgccatgg cgcccgccga gcatctcgac gcggtccagg
     3001 cggccctgat cgagcggctc ggcaaggcga ggatcggcaa cccccgcgag gaaggcgtca
     3061 ccatgggcgc cctcgccagc cgggcccagc tgcgttcggt gcgcgaagcg gtcgggatgt
     3121 tggcaaaagt tgcccgcatc gttcacggcg atcctgagaa atcgcccgtt tccggcggcg
     3181 gagcgttcat ttcgcccatc ctcctgcgca cggattcgcc atggagcgtg gcggaggtgc
     3241 acgacatcga ggcgttcggg ccggtctcga cgatcatgcc ctatcgcgac ctcggcgacg
     3301 ccatcggcct cgccaaccgg ggcatgggca gcctcgccct ctcgctgttc accttcgatc
     3361 cgagcgtcgc ggaggagttc gtgctcggcg ccggcgccta tcacggccgg atgctgatcc
     3421 tcgaccggac cagcgcgaag gaatcgaccg gccacggctc gcccctcccc gtcctcgtcc
     3481 acggcggccc cggccgcgcc ggcggcggcg aggagatggg cgggatccgc ggggtcaccc
     3541 attacatgca gcgaaccgcg ctccagggct ctcccgccgt gctgtcgagc gtcgtcggcc
     3601 agtggctgcc cggcgcgccc aagcccgagg gcgaccggca cccgttccgc aagcgcatcg
     3661 ccgagctcga cgtcggctac acgctgaaga cggcgagccg caccgtcagc ctcgacgaca
     3721 tcgagcattt cgccgcgttc accggcgaca atttctacgc ccatatggac gaggccgcgg
     3781 ccaaggcgaa cccgatcttc gacggccggg tcgcccatgg ctatctcatc ctgagcttcg
     3841 cggccgggct tttcgtcgat cccgatcccg ggccggtcct ggccaattac ggcctcgaga
     3901 acctgcgctt ccagactccg ctctatccgg gcgattcgat gcgcgtcgag ctgacggtga
     3961 agtcgaagag cgtccgcaac gccgagatgg gcgaggtgcg ctgggccgtc tatgtcttca
     4021 accagaagga cgaggtggtc gcctcctacg acctcctcac catgaacacg ccatgatcga
     4081 ccgcaccgcc cccctcctgc tcttcgccct ggccgcctgc ggcgcctccg ccaccgccga
     4141 gccgccggtc gacggctacc ggatcgtgcg cgaatatccg cacgatccga ccgccttcac
     4201 ccagggcctg ctctatctcg acggccgcct ctacgagagc accggcctca atcgccagtc
     4261 caccatccgc gaggtccggc tggaggacgg caaggtgctg cgctcggtct ccatcgcgcc
     4321 ccaatatttc ggcgagggcc tggtcaattg gggccccgac ctgatcagcc tgacctggca
     4381 ggacgggatc ggcttccgct ggaaccgcgc cgacttccgc ccgaaggggc agttccgcta
     4441 cccgggcgag ggctggggcc tgacccagga cggcaagagc atcatcatga gcgacggcac
     4501 cagccagctg cgcttcctcg atccggcgac gatgcgcgag cgccgccgcc tcgccgtcac
     4561 ctaccagggc cagccggtcg agcggctcaa cgagctggag tgggtgaagg gcgagatctt
     4621 cgccaacatc tggatgggcc gccggatcgc ccggatcgac ccgaagagcg gcgtggtcag
     4681 gggatggatc gacctttccg agctggccgc cgagaacgcc aatggcgacc ccgacagcgt
     4741 cctcaacggc atcgcctacg acgccaggcg cgaccggctg ttcgtcaccg gcaagaactg
     4801 ggcgaagctg ttcgagatcg acatcgtccc caagcccaag ccctagtccg gcgcccgcat
     4861 cgccagggcg tgggcggggc gcgacggcga tccggaacgg gcgcctcgcg ccgggggttc
     4921 ttggcgcgaa ccccccgagg agagccccga tgaagatcgc cgaatgcatg accgccgagg
     4981 tcgagaccgt cagcccggac cagccgatcc gcgaggccgc ccagttcatg ctccgcgccg
     5041 atgcgggcgc gatgccggtc ggcgagggcg acaagctggt cgggatggtc accgaccgcg
     5101 acatcgcggt gcgcggcgtg gcggcgggcc gcggcccgga cacgccggtg cgcgaggtga
     5161 tgaccgagga ggtcatctac tgcttcgagg acgacgaggt cgaggaggtg gcgctgaaga
     5221 tgagcgaccg ccaggtccgc cggatgccgg tgctgagccg cgagaacgag accctggtcg
     5281 ggatcgtctc gctcggcgac atcagccgct ccgagcagag cgaggccgct tcggtcgcgc
     5341 tcgacggaat caccgacccg ggcggcgagc acagccagta gcgtccttcg atacgggcct
     5401 tcgacaagct cagtccctac tcaggatgag cggaaatgcg ccagcgctca tcctgagtag
     5461 ccggtgagct tgtcgaaccg gcgtatcgaa ggacggacta ccccgccttg tacgcgccga
     5521 tgccctcgtc gagatggggg ttgttggcct cgcgctgctt ggccgccgcg tcgtcgtaga
     5581 ggaagggcag gaaggcgtag cgcgtgccct tggtcaccgg cgtcgcctcg tgcagcagcg
     5641 agcaggagaa gacgatcgct ccgccggtcg gagcccggta ggtgcgggcg ccgaactcgg
     5701 gaaagcgcag gtcgccgccc tcatagtcgc cggcgttgag gttgatcgtc accgcgaagc
     5761 ggcgatgggc ggtgcccttc gtcgtattgt cgcggtgcgg gcggaaatgc ccggcgcctg
     5821 cctcgtagct gccgacgatg tagcgctcca tccgcgtcgc ctcgaactgg aaggcgcgct
     5881 tgatcatggg caccagccgg gcccggacgc ggtcgcgaag gaggatgcac agcgcctcgt
     5941 cggtgatttc gcggtcccgc cgcatcttgt gctgcgggtc cgtcaccaaa gtcgtcttgc
     6001 cgccga
//
LOCUS       DASVQZ010000314         3808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_64779, whole genome shotgun sequence.
ACCESSION   DASVQZ010000314 DASVQZ010000000
VERSION     DASVQZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64779"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VF547_08690"
     CDS             <1..808
                     /locus_tag="VF547_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262936.1"
                     /translation="SLAAPSITIADLALTGQLAADPNFAGRNAALLNNSGPVKPAGHL
                     IAGGITFTVGSNLFVQNSGTADDLAGLTVGGGGLTIRGGATPITAVGFGRKRNPDGTF
                     TTGNAFFALVDFTKAAGTTYTDDSEVNLCNVNSGACPAPPPPPPSPPSPPPPPSPPPP
                     PPPPPPPPPPPPPPPPPEPPPPPPPPPTPPPPAPPQPPGRTGQPTGAATVLGPLVPNE
                     ARRVEQLLDVSLGPDTVIDEPVASGGDSSLWTGSEEEEEEEEEEGRRPPR"
     gene            805..>3808
                     /locus_tag="VF547_08695"
     CDS             805..>3808
                     /locus_tag="VF547_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8262937.1"
                     /translation="MRHPLLPISSALAALAAASASPAAEPRISLQDSFRLGSGGGAVC
                     SAESASRDPALEDMFDRSYAIVCRDAAAPVGRLYALRVRRGDPAARLAGLRPARCSTA
                     ARTDLESLVGVERLSCTLADGLAYRVYSWRRGNTLFVAEGLAGYDSALRIGLRTLVAD
                     RPVPGNVEVAVTEAGDAAAFARAQAGALDPARARDEAYRRNNAGLFAEAAEFFAVLAA
                     AEPPGAGRAEALVNDAIQQSNLGYYGEADALFERARAAGGGDMVVARMLRNYRSIDLL
                     NRGSPAEALAELDKPLPASATSPAPDSATAIDARTAARINAASPTLRQLGGAAGALLP
                     EERAQILDAQALHLRGTLLRLTGRPGPAAAALREAQAQLGSVRDGRVTSTVWMRAQIH
                     GELAALAEGRGEDSEAEHQHRAAIALLEAGYPNSAALLSARTRLAAHYARSGRGAEAR
                     ALYKQVVDSNAQGGSPSPALHRTLAPYFALLALEGSDQAAVGEMFKASQLLVRPGVAQ
                     TQAVLARELGGGSDEAARLFRQSVNLGRAIERLRAEIGRLADVPEPAAADSEGLAELR
                     AQLARAEQDQAATQSRLADFPRYRVVAGGPMELAELQRALRPGEAYYKMVVVGDAAYA
                     MLATADSASAWKIGAPAAELERDVDALRATIAVQEDGRILTYPFDVERAFALYGKLFG
                     AGADRLASVGHLIFEPDGAMLRLPPNLLVTERAGVDAYLAAARRPGADGFDFTPVRWL
                     GRDRDISTAVSARAFRDVREAAASRAERQYLGLGENAPPEGPQIAQAAVRGLLGDSAA
                     CAWPLAQWAKPISAAELRAAQAAIGRGGAEIVTGRAFSDSAIKARGDLARYRILHFAT
                     HGLVTAPRPECPARPALMTSFGGEGSDGLLSFSEIYDLRLDADLIILSACDTAGKASL
                     AATREAGLRTGGDFALDGLVRAFIGAGGRSVVASHWPVPDDYDATNRLISGLFTAPPG
                     TSTAAALREAQARLMATPETSHPYYWAGF"
BASE COUNT          486 a         1503 c         1336 g          483 t
ORIGIN      
        1 cagcctcgcc gcccccagca tcaccatcgc cgacctcgcg ctcaccgggc agctcgcggc
       61 ggatcccaat ttcgccggcc gcaacgccgc cctcctcaac aatagtggcc cggtgaagcc
      121 cgccgggcac ctgatcgcgg gcggcatcac gttcaccgtc ggcagcaacc tgttcgtcca
      181 gaacagcggc accgccgacg acctcgccgg cctgaccgtc gggggcggcg gcctgacgat
      241 ccgcggcggc gcgactccga tcaccgccgt cggcttcggc cgcaagcgca atccggacgg
      301 gaccttcacc accggcaacg ccttcttcgc cctggtcgat ttcaccaaag ccgccggaac
      361 gacctacacc gacgattccg aggtcaacct ctgcaacgtc aacagcggcg cctgcccagc
      421 ccctccgccg ccgccaccct cgcctccgtc acctcctccg ccgccgtctc cgccgccacc
      481 gccgccgcct ccgccgccgc cccctcctcc tccgcctccg cctccgccgc ccgagcctcc
      541 cccgccgccg ccgccgcctc cgacgccccc gccgccggct ccgccgcagc cgccgggccg
      601 gacggggcag ccgaccgggg cggccacggt gctgggaccg ctcgtgccca acgaagcgcg
      661 gcgcgtggag cagctgctcg acgtcagcct cgggccggac accgtgatcg acgagccggt
      721 ggccagcggt ggcgacagca gcctgtggac cggcagcgag gaggaagagg aagaggaaga
      781 ggaagaaggc aggaggccgc cccgatgagg catccgctcc tcccgatttc gtcggccctg
      841 gccgcgcttg ccgcggcctc ggcgagcccc gccgcggagc cgcggatcag cctgcaggac
      901 agcttccgcc tcgggtccgg cggcggggcg gtctgctccg cggaatcggc ctcgcgcgat
      961 ccggccctgg aggacatgtt cgaccgttcc tacgccatcg tctgccgcga cgcggccgct
     1021 ccggtcggcc gcctctacgc gttgagggtg cggcgcggcg acccggcggc gcggctggca
     1081 gggcttcggc cggcgcgatg ctcgaccgcc gcgcggacgg acctagagtc tctggtcggg
     1141 gtcgagaggc tgagctgcac tctggccgac ggcctcgcct accgcgtcta cagctggcgg
     1201 cgcggcaaca ccctgttcgt cgccgaaggc ctcgccggct acgacagcgc cctgcgcatc
     1261 ggcctgagga ccctcgtcgc cgaccggccg gttcccggca atgtcgaggt cgcggtgacg
     1321 gaggcgggcg acgccgccgc cttcgcccgg gcccaggccg gcgcactcga tccggcgcgg
     1381 gcgcgcgacg aggcctatcg ccgcaacaat gccggcctct tcgctgaggc ggccgagttc
     1441 ttcgccgtcc tcgcggcggc cgagccgccg ggggcagggc gggccgaggc cctggtcaac
     1501 gacgccatcc agcagtcgaa cctgggctat tacggcgagg ccgacgccct gttcgagcgg
     1561 gcgcgggcgg ccggcggcgg cgacatggtg gtcgcccgga tgctgcgcaa ctatcgctcc
     1621 atcgacctgc tcaaccgcgg cagcccggcc gaggccctcg ccgagctgga caagccgctg
     1681 cccgcttccg cgacgtcccc ggctccggac tcggcgaccg cgatcgacgc ccgcaccgcg
     1741 gcccggatca acgcggcctc gcccaccctg cgccagctcg gcggcgcggc cggcgcgctc
     1801 ctgccggagg aacgcgcgca aatcctcgac gcgcaggcct tgcacctgcg cggcacgctg
     1861 ctccggctga cgggccggcc cggcccggcc gcagccgcgc ttcgggaggc gcaggcgcag
     1921 ctcggctccg tccgcgacgg ccgggtgacc tcgacggtct ggatgcgcgc gcagatccac
     1981 ggcgagctcg ccgccctcgc cgagggtcgt ggggaggact ccgaggccga gcatcagcat
     2041 cgcgcggcga tcgccctgct cgaagccggc tatccgaact ccgccgccct gctcagcgcc
     2101 cggactcggc tggcggccca ttacgcgcgc agcggccgcg gggccgaagc ccgagcgctc
     2161 tacaagcagg tcgtcgattc caatgctcag ggcggcagcc cgtcgccggc cctgcaccgc
     2221 acgctggcgc cctatttcgc cctgctggcg ctcgagggaa gcgatcaggc ggcggtcggc
     2281 gagatgttca aggcgagcca gctgctggtg cgccccggcg tcgcccagac ccaggcggtg
     2341 ctggcccgcg agctcggcgg cggcagcgac gaagccgccc gtctgttccg ccagtcggtc
     2401 aaccttgggc gggccatcga gcgcctgcgg gcggagatcg gccgcctggc ggacgtcccc
     2461 gagccggccg cggcggactc cgaagggctg gccgagctcc gcgcccagct cgcccgggcc
     2521 gagcaggacc aggccgcgac gcagtcgagg ctggccgact tcccgcgcta ccgggtcgtc
     2581 gccggcgggc cgatggagct ggccgagctg cagcgggcgc tccgtcccgg cgaagcctat
     2641 tacaagatgg tggtcgtcgg cgacgccgcc tacgcgatgc tcgcgaccgc cgattcggcc
     2701 agcgcctgga agatcggcgc cccggccgcg gagctggagc gcgacgtcga cgcgctgcgc
     2761 gccaccatcg cggtccagga ggacgggcgg atcctcacct acccgttcga cgtcgagcgg
     2821 gccttcgcgc tctacggcaa gctgttcggc gccggagcgg accgcctggc ttcggtcggt
     2881 catctcatct tcgagcccga tggagcgatg ctgcggctgc cgcccaacct gctggtgacc
     2941 gaacgcgccg gagtcgacgc ctacctcgcc gccgcccgtc ggccgggcgc cgacggcttc
     3001 gatttcaccc cggtgcgctg gctcggccgc gaccgggaca tcagcaccgc cgtatcggcc
     3061 cgggccttcc gcgacgtccg cgaggccgcg gcttcccgcg cggagaggca gtatctgggg
     3121 ctcggcgaga acgctccgcc ggagggcccg caaatcgcgc aggcggcggt gcgcgggctg
     3181 ctcggggatt cggcggcctg cgcctggccg ctggcgcaat gggcgaagcc gatctccgcg
     3241 gccgagcttc gcgccgccca ggcggcgatc ggccgcggcg gcgccgagat cgtcaccggc
     3301 cgggcgttca gcgacagcgc gatcaaggcg cgcggcgatc tcgcccgata tcgcatcctc
     3361 catttcgcga cccacgggct ggtcaccgcg ccgcggccgg aatgcccggc ccgaccggcg
     3421 ctgatgacgt cgttcggagg cgagggctcg gacgggctgc tcagcttcag cgagatctac
     3481 gacctgcgcc tcgacgccga cctcatcatc ctctcggcct gcgacaccgc cggcaaggcg
     3541 agcctcgcgg ccacccgcga ggcgggcctc cggaccggcg gcgacttcgc gctcgacggc
     3601 ctggtgcggg ccttcatcgg cgccggcggc cgctcggtgg tcgccagcca ctggccggtg
     3661 cccgacgatt acgacgcgac caatcggctc atctccggtc tgttcaccgc cccgcccggt
     3721 acgtcgacgg cggcggctct gcgcgaagcg caggccaggc tgatggcgac gcccgagaca
     3781 tcgcatcctt attattgggc gggcttcg
//
LOCUS       DASVQZ010000315         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_545426, whole genome shotgun sequence.
ACCESSION   DASVQZ010000315 DASVQZ010000000
VERSION     DASVQZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545426"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="argC"
                     /locus_tag="VF547_08700"
     CDS             complement(<1..811)
                     /gene="argC"
                     /locus_tag="VF547_08700"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX8262938.1"
                     /translation="MIRVAIFGASGFVGAELLRLCAGHPELEPALLFADSQAGQSVEA
                     VHPHLALGYPGRRFEAWAPGALAGIDLVFAALPHGKSQDLAEEILGTGAKFVDLGADF
                     RLSSAEDYARWYGEPHRRPDLLARFAYGIPELDRKAIRGADAVAAAGCYPTSAILACR
                     PLMDLIEPGSLVVDAASGVSGAGKGLKEETHFDTVDGNFTAYGLLGHRHTAEMEMVLG
                     APLLFTPHLAPMNRGILATCYGVAKDACNPLELLRDAYAGEPFIHVSERAPS"
     gene            complement(1187..2035)
                     /locus_tag="VF547_08705"
     CDS             complement(1187..2035)
                     /locus_tag="VF547_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEX8262939.1"
                     /translation="MKLVSKPVEKVWGRTALPFACADAGDALVGELWFNHPGGAASPL
                     LVKFIFTAAPLSVQVHPDDRQARERGLPHGKSECWYIVDAEPGAAVALGLTDALSRDQ
                     LRAAALDGTIARRLAWRPVCAGDFFYIPAGTIHAIGGGVSLIEVQQPSDVTYRLFDHG
                     RARELHIEEAVAVARREPYPDRFATPTTGDGVQVLVSSPHFTLVRVAPGAEPPSRLEG
                     RDRWVVPLDAAVCSRADEAALGECLFVPAGEPLTLAPGASCLIAVEGAVQADRLSEPA
                     RLRKVA"
     gene            complement(2098..>2638)
                     /locus_tag="VF547_08710"
     CDS             complement(2098..>2638)
                     /locus_tag="VF547_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX8262940.1"
                     /translation="GVAQLVRERESYQVVETEGGHIDFAPLDPVEDRILTRLRLRYRR
                     VSVERLASGPGLANLYEALAAIEDRPVLLHNEKALWQAALAGTDTLAAAALDRFCRIL
                     GAVAGDIALAQGAAAVVIGGGLGLRLADLLPGSGFGERFIAKGRFEGRMTRIPVKLIA
                     HPQPGLFGAAAAFAAEHGD"
BASE COUNT          417 a          850 c         1020 g          351 t
ORIGIN      
        1 tcgagggcgc gcgctccgaa acgtggatga agggctcgcc ggcataggcg tcacgcagca
       61 attccagcgg gttgcaggcg tccttggcga cgccgtagca ggttgcgagg atgccccggt
      121 tcatcggggc gaggtgcggc gtgaacagca ggggtgcgcc gagcaccatt tccatctcgg
      181 ccgtgtggcg gtggccgagg aggccgtagg cggtgaaatt gccgtcgacc gtgtcgaaat
      241 gcgtctcctc cttcagcccc ttgccggcgc cggagacgcc cgaggccgca tcgacgacga
      301 ggctgccggg ctcgatcaag tccatcagcg gtcggcaggc gaggatcgcc gaggtcggat
      361 agcagcccgc tgccgcgacc gcgtccgcgc cacggatcgc cttccggtcc agctcgggaa
      421 tgccgtaggc gaagcgtgcg aggagatccg gccggcggtg cggctcgccg taccagcggg
      481 cataatcctc ggcgctcgag aggcggaagt cggcgccgag atccacgaac ttcgcgcccg
      541 tgccgaggat ttcctcagcc aggtcctggg acttgccgtg cggcagggcg gcgaagacga
      601 gatcgatgcc ggcgagggca ccgggcgccc aggcttcgaa ccgccgaccc ggatagccga
      661 gggcgaggtg cgggtgtacc gcctcgacgc tctgccccgc ctggctgtcg gcgaagagga
      721 gagcgggctc gagttccgga tggcccgcgc acagccggag cagttccgct ccgacgaaac
      781 ccgatgctcc aaaaatggcg acgcgaatca tcgcggcgct atgcatatgt gtgcataagt
      841 atgcaagtat cggccttcgc gccgacgttc acggggggaa ccgtcgatca ggccgtccat
      901 tcttcgcgtc gaggagacga tcgatgggcg aggacaagga cgggtgggcg agcgacagct
      961 ctcccgacgg gtaccgcgaa tatagcagct ccggccgcgg cgcggttacg ggaggggcgc
     1021 caaaggaggc gggtgctggg aacagcggag gcgggcgggg cagcgactat ccggtcgccg
     1081 ggcaggacgc ggagcatgaa ggggaagccc ggcccgaatt cccgccgcag ggctggggcg
     1141 aggctggtgg caattaccct ccggccctgg ggccgaacga cgggggctag gcgacctttc
     1201 gaagccgcgc cggctcggag agacggtcgg cctgcaccgc gccttcgacg gcgatcaggc
     1261 aggaggcacc gggcgcgagg gtcagcggct cgccggcggg gacgaacagg cactcgccca
     1321 gcgccgcctc gtcggcgcgc gagcagacgg cggcgtccag cgggacgacc cagcggtcgc
     1381 gcccttcgag ccgcgacggc ggctcggccc ccggcgcgac gcgcaccagg gtgaagtggg
     1441 gcgacgagac gaggacctgc acgccgtcgc cggtcgtggg ggtggcgaac cggtcagggt
     1501 agggctcgcg ccgggcgacg gcgaccgcct cctcgatatg cagctcccgc gcccggccgt
     1561 ggtcgaacag gcggtaggtg acgtcgctgg gctgctgcac ctcgatcagg gaaacgccgc
     1621 cgccgatcgc gtggatggtg cccgcgggta tgtagaagaa atcgcccgcg cagaccggcc
     1681 gccacgccag ccggcgggca atggtgccgt cgagcgcggc ggcgcgaagc tggtcgcggc
     1741 tgagcgcgtc cgtaaggccc agcgcgacgg ccgcgccggg ttcggcgtcg acgatgtacc
     1801 agcattcgct cttgccgtgc ggcaacccgc gctcgcgcgc ctggcggtcg tcggggtgga
     1861 cctgaacgga aagcggggct gcggtgaaga tgaatttgac gagcagcggc gatgccgcgc
     1921 cgccgggatg gttgaaccag agctcgccga cgagggcgtc tcccgcgtcg gcacaggcga
     1981 agggcagggc ggtgcggccc cagactttct caacgggttt cgacacgagc ttcatcgcag
     2041 gcctctcagg gcccgcttat ggcggcgcgg tctcaacaaa gggtgaaggc gggagggcta
     2101 gtcgccatgc tccgcggcga aggcggcggc ggcgccgaac aggccgggtt gcgggtgggc
     2161 gatgagcttg accgggatcc gggtcatccg cccctcgaag cggcccttgg cgatgaagcg
     2221 ctcgccgaag cctgagcccg gcagcaaatc ggcgaggcgc agaccgaggc cgccgccgat
     2281 cacgaccgct gcggccccct gtgcgagggc gatgtcgccc gcgaccgcgc cgaggatgcg
     2341 gcagaagcgg tctagcgccg cggcggcgag ggtgtccgtg ccggcgagcg ccgcctgcca
     2401 cagcgccttt tcgttgtgga ggaggacggg ccggtcctcg atcgccgcca gcgcctcgta
     2461 gaggttggcg aggccgggtc ccgaggcgag tctttcgacc gagacgcggc ggtaacggag
     2521 gcggagccgg gtgaggatgc gatcctcgac tggatcgagc ggggcgaagt cgatgtggcc
     2581 gccctcggtt tccaccactt ggtagctttc gcgctccctg acgagttgcg ccaccccg
//
LOCUS       DASVQZ010000316        13444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_931712, whole genome shotgun sequence.
ACCESSION   DASVQZ010000316 DASVQZ010000000
VERSION     DASVQZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931712"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VF547_08715"
     CDS             complement(<1..641)
                     /locus_tag="VF547_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8262941.1"
                     /translation="MKVAIFGAGSVGCFVGGAWAAAGLEVSFVGRERIGREIKENGLT
                     LTDFSGWRVRLSPEQVDFSTRPAALAGADVIALCVKSTATAAAAKEIARHARKGALVV
                     SFQNGVSNLETLRSVLRNRFEAAAGMVPYNVAALGHGRFHKGVAGDLVAEDLPPLRTL
                     AERIGEGPARLRLSADMPAIAWGKLLINLNNAVNALSGRTLREQLMARDYRRVV"
     gene            complement(772..1218)
                     /gene="queF"
                     /locus_tag="VF547_08720"
     CDS             complement(772..1218)
                     /gene="queF"
                     /locus_tag="VF547_08720"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX8262942.1"
                     /translation="MDLTHLGRSSPLPASPEEARLDYVPNPRPAKPYLVRFTAPEFTS
                     LCPVTGQPDFAHLVIDYAPGATIVESKSLKLFLASFRNHCGFHEDVTVGIAERLVREM
                     EPLWLRIGGYWYPRGGIPIDVFWQSGEPPAGLWVPAQDVPGYRGRG"
     gene            1332..3428
                     /locus_tag="VF547_08725"
     CDS             1332..3428
                     /locus_tag="VF547_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8262943.1"
                     /translation="MATQAEGTAVPAKGTRPDLECDLIMKGGITSGVVYPGAIAQIAD
                     GYRLRAIGGTSAGAIAAAAAAAMEFGRASGLNGGARDRLADVPARLGAATADGTPLLT
                     QLFQPDPGTAPLLAPILDMMRPGFRARLRGALSLLGLPLAGAAVGAAGLLYASGLRQV
                     PPGIVAKSALAIVALVLVAALLVPLLWLLRIRRPLAALAEDFGFCSGMTAARSRDDPP
                     LPSLTEWLHTEIQALAGLPSSEPLTFADLWTADKRAGGASRDAAIQAALADLQAPIED
                     VGLAGCRREIDLALVASDMSRSLSVQFPFVPGGARLYARRTDLDALFPADVVQWMEQR
                     AAPTEAGDLEGVTLDGKAARDLVLPLPRPEDMPIVFAARVSMSFPLLFKAVRLYLLHY
                     RSERRGGGKELLELWLADGGITSNFPVHLFDSPISTRPTFCLNLLYPGDEVHSAGGGD
                     AAGESARPADGASSDPEADHIRMARTNRAELLLLHEVSRGSSLSRLIGWGGRILFTAR
                     QWSDIALMNVPGYRDRIVHIRMLPGEGGLNLAMSQATVRRLDSRGRLAGRVIGERFRP
                     GCTTDPLYGGELKLNWSNHRFVRFRSFIAALEVSVAHFVGGWRKDESSRPSVDEMIDR
                     TAAGTPAPYVGYVFTGNPQRALARDMVSSIEALHDLADRTGSSVDFTSTRHTSPRPKA
                     VLSVRPPLADDPRSEF"
     gene            3495..5012
                     /locus_tag="VF547_08730"
     CDS             3495..5012
                     /locus_tag="VF547_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX8262944.1"
                     /translation="MIVRTAFAALAAIAFAAAAPAPAQDGPPRSKEEQPEKAVPEPIV
                     SVTRHSGTFGGQRIAYTATASETYLKAADGTPKASLFSIAYVKEPRDPTRPITFLFNG
                     GPGSGSLWLHMGAFGPKRVAIPSDARDDAGPPFAILDNPDSLLDATDIVFIDPVGTGF
                     SRPLGKTEAKEYWGVTQDAKSVAEFIRIWLGENGRWNSPKYLGGESYGTTRSAAVVNQ
                     LEGAFNDVSLNGILLISTILDFGAQAYVPGNEMPYVLYVPSMAAAAYHHGKAPADAGT
                     LEGFVQEARDFAAGDYATALIKGQKLQGEERAAIRRRLARFTGLSESFLEAADLRVSD
                     QRFYKELLRDRGLVVGRLDARYTGRDYDNAGETPDNDPSFYGIDAGYTAALNAWVRDG
                     LRFRTDRQYSTIGGVSDWNWDLSDLGGESRYYKNVAPYIGRAMRENSGLRVFVGQGYF
                     DFATPFFGAEHSLSRTGIPQDRIHYSYYGSGHMMYVRDEDRAKLSRDIRAFIRGR"
     gene            5275..5490
                     /locus_tag="VF547_08735"
     CDS             5275..5490
                     /locus_tag="VF547_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8262945.1"
                     /translation="MKNRLKVLRAERDWSQAELGGRLGVSRQAVNAIETGKYDPSLPL
                     AFKLSRLFEVPIEEIFDDGHGASGEAR"
     gene            5456..5911
                     /locus_tag="VF547_08740"
     CDS             5456..5911
                     /locus_tag="VF547_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262946.1"
                     /translation="MTVMGRRARRAEAPLGEGERREAARRRKRWLIVGALALVGFVPG
                     LYLGFEHGAAAAESRPLVWSPTLAAALAGLYLASLLGGGVLLNGVMDEVERQRGYKAV
                     SFAGAALMVVYPTWFLLWKGGFVPEPVHWILFALFWLSLALASLWYRFR"
     gene            5922..6113
                     /locus_tag="VF547_08745"
     CDS             5922..6113
                     /locus_tag="VF547_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262947.1"
                     /translation="MIRKLVAAAVVALIAAPLVSLHQLGAADVGTNVLLLSEDGALVF
                     VVEVRGLVRLEPLVAAIVL"
     gene            complement(6110..8209)
                     /locus_tag="VF547_08750"
     CDS             complement(6110..8209)
                     /locus_tag="VF547_08750"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HEX8262948.1"
                     /translation="MVTTESLLFAIGENGMERDSTSPEAQLVVRLSALLRRKHELYDR
                     LRDQLFYDPQVIEKYATTGGVSLTRNVGDLLELAAYQSAQPGRDAQPPITLGNLLWAF
                     PPDGSVAADLLGQLDIGRDQIVEAILASGAEPEASNSPAEPGSGEPQDRAVGQKRKRP
                     VQKKGLSERTAAAKHAPRNERVSTHVDEPAEIDQLGRRPFAQVLARRIREARDSGEEL
                     EPSAFIVHLHGPWGSGKSSVLNFLERELTGQEPKWIVVKFNAWRDQRLQPPWWTLITT
                     IYAGTRSKLGWAAAPWLRARWLWWRVRADYLPFFAIIACVVGLLLIIRWLGSPIAPDA
                     TPGSAGAEGALDRFGQLLTLAATAVTIGGAIFFGSRSLAMGSQRAAQAYADLKSDPYR
                     PIARLFNRLVKAVNRPIAVFVDDLDRCDGKYVVELLEGIQTLMRGAPVTYVIAADRKW
                     ICSSFEQRYSGFSTSIGEPGRPLGYLFLDKMFQISAGMPQPAAEVQSSYWERLLSGKP
                     QDLAKAAERRAAAEVAADAQAQALTTPEALHELVARAEHSGDQPVIEAARAAAAKRIT
                     SSEMAADTEHRLQPFAPLLEPNPRAMKRLVNAYGMQQATLLLAGRSAPAEALARWTIL
                     ELRWPLLADFLASRPNLVERLRAPLGENDLPETPPSLRGLFGDELVVAVLGPSRGRDR
                     LSAAAIRSILGSAQRER"
     gene            8504..10474
                     /locus_tag="VF547_08755"
     CDS             8504..10474
                     /locus_tag="VF547_08755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8262949.1"
                     /translation="MRLAPFLLLLLTAAARPDANPPLLDPREAEAIAAELSGSAAKRT
                     VQALSLHHRMRGSQGYREAAELIRDRLRDYRLDGVEILSLPSDGKIFYGTQRSRPAWD
                     ASFAELWEQKRQGPGWAGAERVASWAHQPISLAQDSIAGKAETELVDVGAGASAADYA
                     GKDVRGKLVLTSSQPSAVARLAVTERGAAGIVSWAQNQRSAWWGEDESLVRWGHLDTW
                     EDPAFAFMVSPARARAWQARLAKGETVRLRAEVRAGRTPGAYLIPTAVIPGKDRSKEI
                     LFSCHLDHPSPGANDNASGCSGILEIARTFRRLIAEGRLPKPKRTLRFIWPCEIECTI
                     ALLNARKAFASRTLATVHLDMIGGNTDITKGTLGVEGSPPSLPSFVSDVGFAIARWVD
                     DQSLRYANTGAAAFPLLDPEGTRNPLRARIGGFSEGSDHQVWAEGSWRIPVIYVSDWP
                     DIYIHTDKDLPANLDATKMKRAMFIAAASAWVLANADQAQVPAIEQAMGVEAGLRRVE
                     AGRQGRLDDGSATLSRLIWSEKAAARSLLPFGLNYVAERHLDWVPKQPSSQYPSVYRR
                     NPALKGPMDGFGYSWLDDHLKKAGLAKPALLAREPMWDGPSYGYETLNLVDGKRSVEE
                     IAGELAVSVGPAPVEEVAAYLAVLERLGVVQR"
     gene            10483..11325
                     /gene="sseA"
                     /locus_tag="VF547_08760"
     CDS             10483..11325
                     /gene="sseA"
                     /locus_tag="VF547_08760"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEX8262950.1"
                     /translation="MESLVTTEWLERELGRSDLKVIDATLFLPGTPRDPRSEYESEHI
                     PGAVFLDLEEVSDTGSPLPHMMPSEAKFASRMASLGLRDGQRFIVYDNSPLHSAARAW
                     WMLKSFGAHYVAVLDGGLQKWKAEGRPLESGRPPVRHGHFSPSFDPRTVVDKAFVAGL
                     LGTGSHEIVDARGAGRFTGEEAEPRPGMASGHIPGSKNLPQGRFFEADNRWKRGDALR
                     SEFDSAGVDLAKPMVATCGSGVTACVLLFGAHLLGKADARLYDGSWSEWGADPAAPKA
                     TGPA"
     gene            11280..12497
                     /gene="metC"
                     /locus_tag="VF547_08765"
     CDS             11280..12497
                     /gene="metC"
                     /locus_tag="VF547_08765"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX8262951.1"
                     /translation="MGRGSGRAEGDGAGVKGRRPDTRLVHGGRRKEWRGRIVNPPVHR
                     ASTILFDSVAEMRAAAPEFGKPYYGLHGTPTHWALAEALTELEPGAAGTTLYPNGLAA
                     VTAALLTVLSAGDELLMTDSVYGPTRHFCDRFLKRYGIATRYYDPLVGPGISELIGER
                     TRAVFMESPGSLTMEVQDVPAICAAAKARGVATLLDNTWATPLLFPALAAGVDLSILA
                     GTKSVGGHADVMLGSVTATPAWYAKLERTGWDLGHSLGADDAWLGSRGLRTMGVRLRQ
                     HEASALRIARWLEAQPQVAAVLHPALPDCPGHALWARDFKGSSGLFSFALNGGGDSAR
                     TRFVEALQLFGIGYSWGGYESLAVPVDPERLRTATRRTAEGPLVRLQIGLEDADDLIE
                     DLAAALGRWESEA"
     gene            12494..>13444
                     /locus_tag="VF547_08770"
     CDS             12494..>13444
                     /locus_tag="VF547_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX8262952.1"
                     /translation="MRGLFERFGITWPTYGDWMAAAVAVALAAAVWGLAWLAARWLGP
                     RLARFWEHRAGGRGEALAHRMCDLTRYLIAALLVALLLNADEWRPLGAIVLGAALATW
                     AAMLVSGVVRGLQMPRWIAALLAAVTFFALLARAVGGLKPITDTLDRVGFTAGDTRIS
                     LLTVLEVLVTLVALYAVVKLLNRIVGHSIRRASGLDATQQLLGQKLASVAILIAAFFI
                     GIDLVGIDLTALAVFSGAFGLAVGFGLQKTFGNLIAGIILLMDRSIKPGDVIVVGDSF
                     GHVTKIGVRAVSIVTRDGKEHLIPNENLMTQEVENWSYSSR"
BASE COUNT         2002 a         4677 c         4660 g         2105 t
ORIGIN      
        1 accacccgcc ggtagtcgcg cgccatcaat tgctcgcgaa gcgttcggcc ggacagggcg
       61 ttgaccgcat tgttgaggtt gatcagcaat ttgccccagg cgatcgccgg catgtcggcc
      121 gagagacgca gtcgcgccgg accctcgccg atccgttctg cgagcgttcg aagcggcggc
      181 aggtcctcgg cgacgaggtc gccggcgacg cccttgtgga agcgcccgtg gccgagggcg
      241 gcgacgttgt agggcaccat cccggcggcc gcctcgaagc ggttcctcag caccgagcgg
      301 agggtctcga ggttgctgac cccgttctgg aagctgacga ccagggcgcc tttccgggcg
      361 tgccgggcga tctccttcgc ggcggccgcg gtggccgtgc tcttgacgca caaggcgatg
      421 acgtcggcgc cggcgagggc cgccggcctg gtcgagaaat ccacctgctc gggggacagc
      481 cggacccgcc agcccgagaa atcggtgagg gtgaggccgt tctcctttat ctcccggccg
      541 atccgctcgc ggccgacgaa gctgacctcg aggccggccg ccgcccacgc gccgccgacg
      601 aagcagccga ccgagccggc gccgaaaatc gcgaccttca tgcgtgcctc gtacgtgctt
      661 cgagacggca tttcgccggg ctcaatgcct cctcagcatg agcgagtaat cgcaaccgct
      721 catcctgagg aggggctgag cctgccgaag ccccgtctcg aaggaccgtc atcagcctcg
      781 gccgcgatag ccgggcacgt cctgcgcggg cacccacagg ccggccggcg gctcgcccga
      841 ctgccagaag acgtcgatcg gaatgccgcc gcgcggatac caatagccgc cgatgcgcag
      901 ccagagcggc tccatctccc ggaccagccg ctcggcgatg ccgacggtca cgtcctcatg
      961 aaagccgcaa tggtttcgaa aggaggccag gaacagcttc agcgacttcg actcgacgat
     1021 ggtcgcaccc ggcgcatagt cgatgacgag gtgggcgaaa tcgggctggc cggtcaccgg
     1081 gcagagcgaa gtgaattcgg gggccgtgaa gcgcaccaga tagggcttcg ccggccgcgg
     1141 gttcggcacg taatcgagcc tggcctcctc cggcgaggcg ggcagcggac tggagcggcc
     1201 gaggtgggtc aggtccatgg ccggctctct aggcaagcgc ggccgcattg tcaccaaagc
     1261 ggcttgatct tcactgtgaa atcggcaccg cggagcctag gctgcgggcc gaaggcagga
     1321 gaggggcatt gatggcgacg caagccgaag gcacggcggt ccccgccaag gggacccgcc
     1381 ccgatctcga atgcgacctg atcatgaaag gcggaatcac cagcggcgtc gtctatcccg
     1441 gcgccatcgc gcagatcgcg gacggctatc ggctgcgcgc gatcggcgga acctcggccg
     1501 gcgcgatcgc ggccgcggcg gcagccgcga tggaattcgg gcgggcgagc ggcctcaacg
     1561 gcggcgcgcg cgaccggctt gccgacgtgc ccgccaggct gggggcggcc acggccgacg
     1621 gcacgccgct gctgacgcag ctgttccagc ccgatccggg cacggcgccg ctgctcgcgc
     1681 cgatcctcga catgatgcgt ccgggattcc gggcccggct gcggggcgcc ctcagcctgc
     1741 tggggcttcc gctggcgggt gcggcggtcg gcgcggccgg gctcctctac gcgtcgggcc
     1801 tgcgccaggt gccgcccgga atcgtcgcca aatcggccct cgcgatcgtt gccctggtgc
     1861 tggtcgcggc tctgctcgtg ccgctgctgt ggctgttgcg gatccgccgg ccgctcgccg
     1921 ccctcgccga ggatttcgga ttctgttccg gaatgacggc cgccaggtcg cgcgacgatc
     1981 cgccgctgcc ctcgctcacc gagtggctgc acaccgaaat ccaggccctg gccggtctgc
     2041 cgtcgagcga gccgctgacc tttgcggacc tgtggacggc ggacaagagg gccggcggcg
     2101 cgagccgcga cgcggccatc caggctgcgc tcgccgacct ccaggcgccg atcgaggacg
     2161 tcggcctggc cggatgccgc cgcgagatcg acctcgccct ggtcgcttcc gacatgagcc
     2221 ggagcctctc cgtccaattc cctttcgtgc ccggcggagc ccggctctac gctcggcgca
     2281 ccgatctcga cgccttgttt ccggccgacg tggtccaatg gatggagcag cgggcggcgc
     2341 cgaccgaggc cggcgacctg gaaggcgtga cgctcgacgg caaggccgcg cgggatcttg
     2401 tccttccgct gccacgcccc gaggacatgc cgatcgtgtt cgcggcgcgc gtttcgatga
     2461 gctttccgct gctgttcaag gcggttcgac tctatctgct ccattaccgc tccgagcgcc
     2521 gggggggcgg caaggagctg ctcgaactgt ggctggcgga cggcgggatc acgtccaact
     2581 tcccggtcca tctgttcgac tcgccgatct cgacccgccc gaccttctgc ctcaacctcc
     2641 tctacccggg cgacgaggtc cattcggcgg gcggcggcga cgcggcgggg gaatcggcgc
     2701 gacccgcgga cggtgcctcc agcgacccgg aggcggatca tatccgaatg gcccgcacca
     2761 accgcgccga gctgctgctg ctccacgagg tgagccgggg ctcctccctg tcgcggctga
     2821 tcgggtgggg cgggcgaatc ctcttcaccg cgcgccaatg gtcggacatc gctctgatga
     2881 acgtgcccgg ctatcgcgat cggatcgtcc atatccggat gctgcccggg gagggcgggc
     2941 tcaacctcgc gatgagccag gcgacggtcc gccggctcga ttcgcgcggc cggctggccg
     3001 gcagggtcat cggcgagcgc ttccgcccgg gctgcacgac cgatccgctc tatggcggcg
     3061 agctcaagct caactggtcg aaccaccgct tcgtgcgctt ccgctcgttc atcgccgcgc
     3121 tcgaggtcag cgtggcgcat ttcgtcggcg gctggcgaaa ggatgaatcg agccggccca
     3181 gcgtcgacga gatgatcgac cggaccgccg cagggactcc ggcgccctat gtcggctacg
     3241 tcttcaccgg caacccgcaa cgcgctctgg cgcgcgacat ggtgagctcg atcgaggcgc
     3301 tccacgacct cgccgaccgc accggaagct cggtcgactt cacctccacc cgccacacca
     3361 gccctcggcc gaaggcggtg ctcagcgttc gcccgccgct cgccgacgat ccccgcagcg
     3421 agttctgatc cgccggcttt cgccgggcgc ggcgccgtgc gatagcgcgt cggacgcgca
     3481 atccgaggtt cccgatgatc gtccgcaccg ccttcgccgc actggccgcc atcgccttcg
     3541 ccgccgccgc gcccgcgcca gcccaggacg ggccgccccg ttcgaaagag gaacagcccg
     3601 agaaggccgt ccccgaaccg atcgtctcgg tcacccgcca cagcggcacg ttcggcggcc
     3661 agcgcatcgc ctataccgcg accgcgagcg agacctacct caaggccgcg gacggcacgc
     3721 ccaaggccag cctcttctcg atcgcctacg tgaaggagcc ccgcgatccg acccgcccga
     3781 tcaccttcct gttcaacggc ggccccggct cggggtcgct gtggctgcac atgggcgcct
     3841 tcgggccgaa gcgggtggcg atcccgtccg atgcgcgcga cgacgcgggc ccgcccttcg
     3901 cgatcctcga caatcccgac agcctgctcg acgccaccga catcgtcttc atcgatcccg
     3961 tcggcaccgg cttcagccgc ccgctcggca agaccgaggc caaggaatat tggggagtca
     4021 cccaggacgc caagtcggtc gccgagttca tccggatctg gctgggcgag aacgggcgct
     4081 ggaactcgcc caaatatctc ggcggcgaaa gctacggcac cacccgctcg gccgcggtcg
     4141 tcaaccagct cgagggcgcg ttcaacgacg tctcgctgaa cggcatcctc ctcatctcga
     4201 ccatcctgga tttcggcgcc caggcttacg tgccgggcaa cgagatgccc tatgtcctct
     4261 acgtgccgtc gatggccgcg gcggcctatc accacggcaa ggcccccgcc gacgccggga
     4321 cgctggaggg cttcgtccag gaggcgcgcg acttcgccgc gggcgactat gcgacggccc
     4381 tgatcaaggg ccagaagctt cagggggagg agcgcgccgc gatccgccgc cggctcgcgc
     4441 gcttcaccgg gctttccgag agcttcctcg aggcggcgga cctgcgggtc agcgaccagc
     4501 gcttctacaa ggagcttctc cgcgaccgcg gcctcgtcgt cggccggctg gacgcgcgct
     4561 acaccggccg cgactacgac aatgccggcg agacgccgga caacgatccc agcttctacg
     4621 gcatcgacgc cggctatacc gcggcgctca acgcctgggt ccgcgatggc ctcaggttcc
     4681 ggaccgaccg ccaatattcc accatcggcg gggtcagcga ctggaactgg gacctgagcg
     4741 acctcggcgg cgagagccgc tactacaaga acgtcgcgcc ctatatcggc cgggcgatgc
     4801 gcgagaattc gggcctccgc gtgttcgtgg gccagggcta tttcgatttc gccacgccct
     4861 tcttcggcgc cgagcattcc ctgtcgcgca ccggcatccc gcaggatcgg atccactatt
     4921 cctactacgg ctccggccac atgatgtacg tccgggacga ggaccgggcc aagctcagcc
     4981 gcgacatcag ggcgttcatc cgggggcggt gagagaggga cccgggagga atctcggtcc
     5041 acccgttcgg gctgagcttg tcgaagccct tactttgatc ttggcacgga agggagggct
     5101 tcgacaggct cagcccgaac ggatatgaat gctattgatg ctggcttttt gccaaagata
     5161 cctgacaaag tgtaatgtaa acttgatatt ctgtcgggct tccactactt gattcgcatg
     5221 ccggcggatg ggctgccggc gggacgaggc gagggcggcg gagttgggga ttaggtgaag
     5281 aaccggctga aggtcctgag ggcggaacgc gactggagcc aggcggagct tggcggccgg
     5341 ctcggagtct cccgccaggc ggtgaacgcg atcgagaccg gcaagtacga tccttcgctg
     5401 cctctggcct tcaagctcag ccgcctgttc gaagtcccga tcgaggagat attcgatgac
     5461 ggtcatgggg cgtcgggcga ggcgcgctga agcgccgctc ggcgaggggg agcgccgcga
     5521 ggcggcgcgg cggcgcaagc ggtggctgat cgtcggggcg cttgccctgg tcggcttcgt
     5581 gcccggcctt tacctggggt tcgagcatgg cgccgcggct gccgagagcc ggccgctggt
     5641 ctggtcgccg acgctggccg cggcgctcgc cggcctctac ctcgcctcgc tgctgggagg
     5701 cggcgtcctg ctgaacggcg tcatggacga ggtggagcgg cagcgcggct acaaggcggt
     5761 gagcttcgcc ggcgccgcgc tgatggtcgt ctacccgacc tggttcctgc tctggaaggg
     5821 cggcttcgtc cccgagccgg tccattggat cctcttcgcc ctgttctggc tgagcctcgc
     5881 gctcgccagc ctctggtatc gtttccgcta ggatgtccgg catgatccgc aaactcgtcg
     5941 ccgcggccgt ggtcgccctg atcgcagcgc cgctcgtctc tctccaccag ctcggcgcgg
     6001 ccgacgtcgg aaccaacgtc ttgctgctca gcgaagacgg cgccctggtc ttcgtcgtcg
     6061 aagtgcgcgg ccttgtccgg ctcgagccgc tggtcgccgc aatcgtcctc tagcgctcgc
     6121 gctgggccga tcccaggatc gaccttatgg ccgcggcgga aaggcggtcc cgaccccggc
     6181 ttgggccgag cacggcgacg acgagctcgt cgccgaacag cccgcgcagc gacggcggcg
     6241 tctccggcag atcgttctcg ccgagcgggg cgcgcagccg ctcgacgaga ttcgggcggc
     6301 tggcgaggaa gtccgcgagc aggggccagc gcagttcgag gatggtccag cgagcaagcg
     6361 cttccgccgg cgcgctgcgc ccggcgagca gcaaggtcgc ctgctgcatc ccataggcgt
     6421 tgacgaggcg cttcatcgcc cggggattcg gctcgagcag cggcgcgaag ggctgcagcc
     6481 gatgctcggt gtccgccgcc atttccgaac tggtgatgcg cttggccgcc gccgcccggg
     6541 cggcctcgat gaccggctgg tcgccgctgt gctcggccct ggcgacgagc tcgtgcaggg
     6601 cctccggcgt ggtcagcgct tgcgcctgcg cgtcggccgc aacttcggcg gcggctcgcc
     6661 tttcggccgc cttcgccaga tcctgcggct tcccgctcag aagcctctcc caataggagg
     6721 actgaacttc agcggcgggc tggggcatgc ccgcggagat ctggaacatc ttgtcgagga
     6781 acaaatagcc gagtgggcgg cccggctctc cgatcgacgt cgagaagccg gaatagcgct
     6841 gctcgaagct ggagcagatc cacttgcggt ctgcggcaat gacgtaggtg acgggggcgc
     6901 cgcgcatcaa agtctggatg ccttcgagca gctcgacgac atatttaccg tcgcagcggt
     6961 cgaggtcgtc gacgaacacc gcgatcggcc ggttcactgc cttgactagg cgattgaaca
     7021 atcgggctat cggccggtaa ggatcactct tgagatccgc ataggcctgg gcggcgcgct
     7081 gcgaccccat ggcgagcgag cggctgccga agaagatcgc gccgccgatg gtgaccgcgg
     7141 tggcggcgag ggtcagaagc tggccgaacc ggtcgagagc gccctccgcg ccggccgagc
     7201 cgggagtcgc gtccggcgcg atcggcgatc ccagccagcg gatgatgagc agcaggccca
     7261 cgacgcaggc gatgatggca aagaacggta gataatcggc gcgcacccgc caccacagcc
     7321 agcgtgcccg aagccagggc gcggcggccc agccgagctt cgatcgcgtt cccgcataaa
     7381 tggtcgtgat cagggtccac cagggcggct gcaggcgctg gtcccgccag gcgttgaact
     7441 tgacgacgat ccatttcggc tcctgcccgg tcagctcccg ttcgaggaag ttaagcaccg
     7501 aggatttgcc cgagccccag ggaccgtgga gatggacgat gaaagcggaa ggctcgagct
     7561 cctcgccgct gtcgcgggct tcgcgaatcc tgcgggcaag cacctgggcg aagggcctcc
     7621 gcccgagctg gtcgatctcg gccggctcgt cgacatgggt cgagacgcgc tcgttgcgtg
     7681 gagcgtgctt cgcggccgcc gtccgctccg aaagcccctt tttttggacc ggccgcttgc
     7741 gcttctggcc gacagcgcga tcctgaggct ctccgcttcc gggctcggca ggcgaattcg
     7801 acgcctccgg ctccgctccc gaggcgagga ttgcctcgac gatctggtcc ctgccgatat
     7861 cgagctggcc tagaaggtcg gcggcgacgc tgccgtcggg cgggaaggcc cagagcagat
     7921 tgccgagggt gatgggcggc tgggcatcgc ggccgggctg cgccgactga taggcggcaa
     7981 gttccagcaa gtcgccgaca ttccgggtga gcgagacgcc accggtggtc gcgtattttt
     8041 cgatgacctg cggatcgtaa aacaactgat cgcgcagccg atcgtagagt tcgtgtttac
     8101 gccggagcag ggcgctgaga cggaccacga gctgcgcttc gggagacgtg gagtcccgct
     8161 ccatgccgtt ctcaccgatc gcgaagagaa ggctctcggt ggtcaccatc aacccgccct
     8221 tccgcgccgt atacgagagt ctgcccgcgg tccggaacag ggcgaagacc tcctcggtcg
     8281 cggtacggcg ttgccggtgc agccatatcg agagctgttc cgcggcctcg gcggacgcct
     8341 gttccggcgt gattttctcg gattccaaga agcctcccct cgcttgccgc gcacccctgt
     8401 atatcagcgg aggccgcaga cgtaacggct ccggccgaag cagtgcggct ggacatcgcc
     8461 gcggcagagc gacaaggaaa gcgcccgagc tctggagaag ccgatgcgtc tcgcgccctt
     8521 ccttttgctc ctgcttacag cggcggccag gccggacgcc aacccgcctc tgctcgatcc
     8581 gagggaggcc gaggcgatcg ccgcggagct gtcgggcagc gcggccaagc ggacggtgca
     8641 ggcgctgtct cttcaccacc ggatgcgcgg ctcgcaaggc tatcgcgagg cggccgagct
     8701 gatccgcgac cgccttcgcg actatcggct ggacggggtg gagatcctct cgctgccgtc
     8761 ggacggcaag atcttctacg gtacgcagcg ctcccgcccg gcctgggacg ccagcttcgc
     8821 cgagctctgg gagcagaaaa ggcaggggcc ggggtgggcc ggcgccgagc gagtcgcctc
     8881 ctgggcccac cagccgatca gcctcgccca ggacagcatc gccgggaagg ccgagacgga
     8941 gctggtcgac gtcggcgccg gggcgtcggc ggccgactat gccggcaagg acgtgcgcgg
     9001 caagctggtc ctgacctcgt cgcagccgag cgcggtcgcg cgcctcgccg tcaccgagcg
     9061 aggcgccgcg ggcatcgtct cctgggcgca gaaccagcgg tcggcctggt ggggcgagga
     9121 cgagagcctg gtccggtggg gccatctcga cacctgggaa gaccccgcct tcgcgttcat
     9181 ggtctcgccg gcccgggcgc gcgcctggca ggcccggctt gcgaaggggg agacggtgcg
     9241 gctgcgcgct gaggtgaggg cggggcggac gcccggcgcc tacctgatcc cgaccgcggt
     9301 cattcccggc aaggaccggt cgaaggagat ccttttctcc tgccacctcg accacccctc
     9361 gccgggggcg aacgacaatg ccagcggctg ctccggaatc ctcgagatcg cccgcacctt
     9421 ccggcggctg atcgccgagg gccggctgcc gaagccgaag cggacgctcc gcttcatctg
     9481 gccgtgcgag atcgagtgca ccatcgctct gctcaacgcg aggaaggcgt tcgcgagccg
     9541 gaccctggcg acggtccatc tcgacatgat cggcggtaat acggacatca ccaagggcac
     9601 gctcggcgtc gaagggtcgc cgccgagcct gcccagcttc gtttccgacg ttggcttcgc
     9661 catcgcccgc tgggtggacg atcagtcgct gcgctacgcc aatacggggg cggccgcttt
     9721 cccgctgctc gatccggagg gcacgaggaa cccgctccgc gcccggatcg gcggcttctc
     9781 ggagggctcg gaccaccagg tctgggccga gggcagctgg cggatcccgg tcatctacgt
     9841 ctccgactgg cccgacatct acatccacac cgacaaggac ctgccggcca atctcgacgc
     9901 caccaagatg aagcgcgcga tgttcatcgc cgcggcttcg gcctgggtgc tggccaatgc
     9961 cgaccaggcg caggttccgg ccatcgagca ggcgatgggg gtcgaggccg gcttgcggcg
    10021 ggtcgaggcc gggcggcaag ggcgcctgga cgacggcagc gccaccttgt cgcgactcat
    10081 ctggtccgaa aaggccgccg cccgttcgct gctcccgttc gggctgaact acgtcgcgga
    10141 acgccacctg gactgggttc cgaagcagcc ctcctcccaa tatccttcgg tctatcgccg
    10201 caaccctgcg ctgaaggggc cgatggacgg cttcggctat tcatggctcg acgatcatct
    10261 gaagaaggcg gggctcgcca agcccgccct gctcgctcgc gagccgatgt gggacggccc
    10321 gagctacggt tatgagacgc tcaatctcgt cgacggcaag cgcagcgtcg aggagatagc
    10381 cggcgagctg gccgtcagcg tcggccccgc gccggtcgag gaggtggcgg cctatctggc
    10441 ggtgctggag cggctcggcg tggttcagcg ttaggaggga tgatggaatc gctcgtcacc
    10501 accgaatggc tggagcggga gctcggcagg agcgacctca aggtgatcga cgcgaccctg
    10561 ttcctgcccg gcacgccgcg cgacccgcgc tccgaatatg aatcggagca cataccgggt
    10621 gccgtcttcc tcgacctcga ggaggtttcg gacacgggaa gcccgctgcc gcacatgatg
    10681 ccatccgagg ccaagttcgc aagccgcatg gcctccctgg gcctgcgcga cggccagcgg
    10741 ttcatcgtct acgacaacag cccgctgcac agcgccgccc gcgcctggtg gatgctgaag
    10801 agcttcggag cgcattacgt cgccgtcctc gacggcggcc tgcagaagtg gaaggccgag
    10861 gggcggcccc tggaaagcgg ccggccgccg gtgcggcacg gccatttcag cccgagcttc
    10921 gacccgcgga cggtggtcga caaggccttc gtcgcgggcc tgctcggcac cggcagccac
    10981 gagatcgtgg acgcgcgcgg cgccggccgc ttcaccggcg aggaggcgga gccgcggccg
    11041 ggcatggctt cgggccacat accgggctcg aagaacctgc ctcagggccg gtttttcgag
    11101 gccgacaatc gctggaagcg cggcgatgcg cttcgctcgg agttcgactc ggccggagtc
    11161 gacctggcca agccgatggt cgcgacctgt ggctccggag tcaccgcctg cgtgctgctg
    11221 ttcggggcgc atctgctcgg caaggcggat gctcggctct acgacggaag ctggtccgaa
    11281 tggggcgcgg atccggccgc gccgaaggcg acggggccgg cgtgaagggg cggcggcccg
    11341 acacgaggct cgtccatggc gggcgtcgga aggaatggcg cggccggatc gtcaacccgc
    11401 ccgtccaccg cgcctcgacc atcctcttcg acagcgtcgc cgagatgcgc gccgccgcgc
    11461 cggagttcgg caagccctat tacgggctcc acggcacgcc cacgcactgg gcgctcgccg
    11521 aggcgctgac cgagctcgag ccgggagcgg ccggaaccac gctctatccc aacggcctgg
    11581 cggccgtgac ggcggcgctc ctgacggtgc tgtcggcggg cgacgagctg ctgatgacgg
    11641 acagcgtcta cggtccgacc cggcatttct gcgatcgctt cctgaagcgc tacggcatcg
    11701 ccacgcgcta ttacgacccg ctcgtcggcc ccggcatctc cgagctgatc ggcgagcgca
    11761 cccgggccgt cttcatggag agccccggct cgctgaccat ggaggtgcag gacgtgcccg
    11821 cgatctgcgc cgccgcgaag gcccggggcg tcgcgacgct gctcgacaac acctgggcga
    11881 cgccgctcct cttccccgcg ctcgccgccg gagtcgacct gtcgattctc gccggcacca
    11941 aatctgtcgg cggtcacgcc gacgtgatgc tcggctcggt caccgccacc ccggcctggt
    12001 atgcgaagct cgagcggacg ggctgggacc tcggccattc gctcggcgcg gacgacgcct
    12061 ggctcggctc gagaggcctg cgcacgatgg gcgttcggct ccgccagcac gaggcgagcg
    12121 cgcttcggat cgcccgctgg ctcgaggctc agccgcaggt ggcggccgtg ctccacccgg
    12181 ccttgcccga ttgccccggc cacgcgctct gggcgcggga cttcaagggc tcgtccggcc
    12241 ttttctcctt cgcgctgaac ggcggcggcg attccgcgcg cacccgcttc gtcgaggcgc
    12301 tgcagctgtt cggcatcggc tacagctggg gcggctatga gagcctggcc gtgccggtgg
    12361 atcccgagcg cctgcggacc gccacgcgcc ggacggccga ggggccgctg gtccgcctgc
    12421 agatcggtct cgaggacgcc gacgatctga tcgaggatct tgcggccgcc ctcggccgct
    12481 gggagagcga ggcatgaggg gcctgttcga acggttcgga atcacctggc cgacctacgg
    12541 cgactggatg gcggcggcgg tggccgtcgc cctcgccgcc gccgtctggg gcctcgcctg
    12601 gctggccgcg cgctggctgg gcccacgcct ggcaaggttc tgggagcatc gcgccggcgg
    12661 ccgcggcgag gcgctggccc accgcatgtg cgatctcacc cgctatctga tcgcggccct
    12721 gctcgtcgct ctgcttctca acgcggacga atggcggccg ctgggcgcga tcgtgctggg
    12781 cgcggcgctc gccacctggg cggcgatgct ggtctcgggg gtcgtgcgcg ggctccagat
    12841 gcctcgctgg atcgccgccc tgctcgccgc cgtcaccttc ttcgccctgc tcgcgcgcgc
    12901 ggtcgggggc ctgaagccga tcaccgacac gctcgaccgg gtcggcttca ccgccggcga
    12961 cacgcggatc tcgctgctga ccgtgctcga ggtgctggtg acgctggtcg ccctctacgc
    13021 ggtggtgaag cttctcaacc ggatcgtcgg ccattcgatc cgccgagcgt cggggctcga
    13081 tgccacccag cagctgctcg gccagaagct cgccagcgtc gccatcctga tcgccgcctt
    13141 cttcatcggc atcgatctgg tcgggatcga cctcaccgcg ctggcggtct tctccggcgc
    13201 cttcggcctg gcggtcgggt tcgggctcca gaagacgttc ggcaacctca tcgcgggaat
    13261 catcctgctg atggaccggt cgatcaagcc gggcgacgtc atcgtggtcg gcgacagctt
    13321 cggccatgtc acgaagatcg gcgtccgcgc cgtctcgatc gtcacccgcg acggcaagga
    13381 gcatctgatc ccgaacgaga atctgatgac ccaggaggtc gagaactggt cctattccag
    13441 ccgc
//
LOCUS       DASVQZ010000317        10209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_166497, whole genome shotgun sequence.
ACCESSION   DASVQZ010000317 DASVQZ010000000
VERSION     DASVQZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166497"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VF547_08775"
     CDS             complement(<1..874)
                     /locus_tag="VF547_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8262953.1"
                     /translation="MLGAAEGWMAGMTRRSAIKAGTAAAAVAAVDAPALASSGSADAV
                     VVGAGVFGTCTARALQRTGRKVVLVDAWGPAHARASSGGESRLTRGSYGADEVYTRMA
                     WESLAEWRALSARSELPLLHRAGVLFFFPTPEDYVHRTIEVHRRLKLPTQAMDRREMR
                     RRFPMIDFDGVELGLWEPEFGALMARRAVQTMVAEFVAAGGEYRQSAVEPPGRGEGPL
                     ARLGLEDGSTLSADRFVFACGPWLPKLFPDLLGRRIFPTRQEVFFFRPQAGDGRFALG
                     RLPAWADFNAGDIFY"
     gene            1027..3447
                     /locus_tag="VF547_08780"
     CDS             1027..3447
                     /locus_tag="VF547_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8262954.1"
                     /translation="MARLWNRRRFAKTAFLAGASLAACLAAASPARAQDDSTATPPGQ
                     AAGEDGETIVVTATKRETTLLEVPFSINAQTEEDIQRLGAGNIEDLSRNVAGLTVQNL
                     GPGQSQVSIRGVSAGQIIRDQPGVKEQVGVYLDESVVSLSLFQPDLDLFDLNRVETLR
                     GPQGTLFGSGSVGGTIRYITNQPTLSRVEGVVESNLHLVEDGDFGGHIKGAINIPAGD
                     HAAVRVVAYHTEYAGFVDARHEAGIADDVNDGRRTGGRIAVRIEPSDRITVTPRLVYQ
                     EVRSHGFNRQEVYNLYANPFTTTRPAVQLGEREQFLRLRERFSDNTLLADLTASADLG
                     GIELTSVTSYINRDILVSRDASALTGSVSVDLGFPAGGVELPSNLVDTTDLKTFTQEL
                     RLSSNNEGPFQWLIGAFYSDVDRIYAQRLPTPGYDAFINARFGPGTAAAVANGFPANS
                     PYNADLPYDIQQKALFGEASYDITEALTATAGLRYYDFKEVRSFRSGGLFSNGDNQTD
                     ETKSDGLSPRLLLSYEASDNVIINLQASKGFRLGGVNDPLNEGLCSAQDVIDYGGFDT
                     YDDESLWNYEAGVKSQFGPISVNAAAFYTDISNLQVTADAGSCSSRVVFNADAHTMGL
                     EFELSARPVQGLNLSLAGSLVEAEFDETRLKQNGQVLEGIRDGNRLPSVPKFQLAASA
                     FYTFPIGEGEDEAYFGATFQHVGTRFTQPGDQENNPRTFVHGLPFGGAPASASTTLDL
                     KLPDYQLVNVSAGIEMANGFDLSVYVNNLFDENAILAFDRERGGRARLGFHIGQPRTF
                     GVTARKRF"
     gene            complement(3466..3891)
                     /gene="mscL"
                     /locus_tag="VF547_08785"
     CDS             complement(3466..3891)
                     /gene="mscL"
                     /locus_tag="VF547_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX8262955.1"
                     /translation="MSGFREFIAKGNVVDLAVAVIIGAAFAAIVTSLTSDIIMPVIGW
                     LFGGLDFSSYFVRLGPIPADYAGDPNNYAALKAAGVPMIGYGQFATAVVAFLILAFVI
                     YQLVKAASRFQRTPEAAPAGPSEELVVLREIRDALRARP"
     gene            complement(4035..4505)
                     /locus_tag="VF547_08790"
     CDS             complement(4035..4505)
                     /locus_tag="VF547_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX8262956.1"
                     /translation="MRKMLESHIFAALAGAVLLAGCDRPPPDGTVRVRDAWIRLPAAK
                     SRPGSAYFRMEAGSEGTRLVGVSSPAARWIELHETSMSGTSAKMKRHKEIEFPSRGEL
                     AFEPGGRHAMLFGLSPKVKVGDRVPLTFSFNVAPPITLDAEVRGAADEGPHAGH"
     gene            4642..6567
                     /gene="dnaK"
                     /locus_tag="VF547_08795"
     CDS             4642..6567
                     /gene="dnaK"
                     /locus_tag="VF547_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8262957.1"
                     /translation="MARVIGIDLGTTNSCVAVMEGGKPKVVENAEGARTTPSIVAFAK
                     DGERLVGQPAKRQAVTNPETTVFAVKRLIGRRFDDPITKKDTELVPYKITRGPNGDAW
                     VNAGAKDYSPSQISAFILQKMKETAEGYLGETVTQAVITVPAYFNDAQRQATKDAGQI
                     AGLEVLRIINEPTAAALAYGLDKQDGKTIAVYDLGGGTFDISVLEIGDGVFEVKSTNG
                     DTFLGGEDFDAKIVDFLADEFKKQEGIDLRSDRLALQRLKEAAERAKIELSSAQTTEV
                     NLPFITADQNGPKHLVKAISRADLERLVEDLIKRTLEPCKKALADAGVKAEDIAEVVL
                     VGGMTRMPRVRQVVQDFFGKEPHTGVNPDEVVAMGAAIQAGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRMIDRNTTIPTKKSQTFSTAEDNQNAVTIRVFQGEREMAIDNKILG
                     QFDLVGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGKEQQIRIQASGGLSDADID
                     KMVREAESFAEEDKKRRAAAEAKNSAEGLAHSTERQLAEHGDKIDEALKAEIQTAIDE
                     TKQAVESGDVEAMQAKTSALSQVAMKLGQAIYEKEQANAGGSGGDTEAAQGAEPGGGE
                     EEVVDAEFSEVDDENKG"
     gene            6701..7819
                     /gene="dnaJ"
                     /locus_tag="VF547_08800"
     CDS             6701..7819
                     /gene="dnaJ"
                     /locus_tag="VF547_08800"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8262958.1"
                     /translation="MAEQDYYELLQVERGADERTLKSAYRRLAMECHPDRNPGCKDSE
                     ARFKAINEAYDCLKDPQKRAAYDRFGHAAFRNGGMGGAGAQDFGSFSDIFENIFGEFM
                     NGQQADRRSNALRGSDLRYDLEISLEDAYAGKQVELRIDTTARCEPCGGSGAKAGTQA
                     RTCTMCHGRGQVRASQGFFVVERTCPTCRGVGEVIADPCERCRGEGRIERQRSLDVRI
                     PAGVDQGTRIRLAGEGEAGVRGGPNGDLYIFVHLARHPVFQREGTTLFVRAPVSITTA
                     ALGGCIEVPGLDRQKHSIAIPAGIQSGKQLRQRGAGMPVLNGRGHGDMVIQVEVETPT
                     RLTARQKELLEAFRETETGEECPASKSFFGKLKGLFDA"
     gene            8015..9049
                     /locus_tag="VF547_08805"
     CDS             8015..9049
                     /locus_tag="VF547_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8262959.1"
                     /translation="MRFLLPLLALVAASCATARQTPPTFANPVIDSDFPDPAILRASD
                     GFYYVYATQTERDGKWVNLQVARSRDLVAWEHLGDALPAKPGWASKTQDFWAPHVAEH
                     GGRYYLYYSAKPDEALIDDKKGLCLAAATADRPEGPFTDMGRPLLCGASFVNIDPMSY
                     DDPATGRRLLYWGSGFQPIKVQELAPDRLSFAPGSAPIDLVPADPKTDYRKLVEGAWI
                     VLRGGYYYLFYSGDNCCGPGVHYAVMVARSRSATGPFELQPGTLLAQSPKWIGPGHNS
                     VVEDDDGESWIVYHAVDSARPRTKPTDEVNTRRVMLIDHLEWTDEWPRVKGPTSGPQR
                     RRYPERYAPD"
     gene            9074..10003
                     /locus_tag="VF547_08810"
     CDS             9074..10003
                     /locus_tag="VF547_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8262960.1"
                     /translation="MRLPAFALAALLAAPAFAQQDLSKVEVKVERIAPGVAVLLGAGG
                     NIGLSYGADGNIVVDDQYAPMSEKILAAIATVDPDPVRFVVNTHWHGDHTGGNEAMGK
                     RGAVIVAHSNVRERMSSEQFIAALDDRVPASPPAALPVVTFEDGVALHLNGDTLHVVH
                     VANAHTDGDSLVHWQKANVLHMGDTFFHKFSLPFIDLSSGGSIDGLIAAVDKGLAWSN
                     PATRIIPGHGPVASRAELAAYKAMLVDVRAKVAAGIRARRSLAQIQATKPAAPYGMAD
                     GFIKPDQFVAFVHESLTKPAKAGHAHGGETHSH"
     gene            complement(10012..>10209)
                     /locus_tag="VF547_08815"
     CDS             complement(10012..>10209)
                     /locus_tag="VF547_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262961.1"
                     /translation="RDEHGALGSCLTRAENGDFIAFARWPDEQARAAAFAARGPLEPW
                     PGILHVEETKLSVVEDLLTRR"
BASE COUNT         1745 a         3633 c         3379 g         1452 t
ORIGIN      
        1 cgtagaagat gtcgccggcg ttgaagtccg cccaggcggg cagccggccc agggcgaagc
       61 gcccgtcgcc cgcctgcggg cggaagaaga acacctcctg gcgggtcggg aagatgcgcc
      121 ggccgagcag gtcggggaac agcttcggaa gccagggccc gcaggcgaag acgaaacggt
      181 cggccgacag ggtcgagccg tcctcgaggc cgagccgggc gagcggaccc tccccgcgcc
      241 caggaggctc gaccgccgac tggcggtact cgccgccggc cgcgacgaac tccgcgacca
      301 tcgtctgcac cgcccgccgc gccatcagcg ctccgaattc cggttcccac aggccgagct
      361 cgaccccgtc gaaatcgatc atcgggaagc ggcggcgcat ctccctgcga tccatggcct
      421 gggtcggcag cttcagccgg cgatgcacct cgatggtgcg atggacgtag tcctccggcg
      481 tcgggaagaa gaacagcact ccggcccggt ggagcagcgg cagctcggac cgcgccgaga
      541 gcgcgcgcca ctccgcaagc gactcccagg ccatgcgcgt gtagacctcg tcggcgccgt
      601 agctgccccg ggtcagccgc gactccccgc ccgaggaggc gcgggcatgg gcgggccccc
      661 aggcgtcgac cagcaccacc ttgcggccgg tccgctgcag cgcccgggcc gtgcaggtgc
      721 cgaacacgcc ggcgccgacg acgacggcgt cggcggaccc ggacgaggcg agggccgggg
      781 catcgaccgc cgcgaccgcc gccgccgccg tccccgcctt gattgccgac cggcgcgtca
      841 tccccgccat ccacccctcc gcggcgccca gcatttgggc tggccggcca atagtcaagc
      901 cgtccgcccc ctgtatcgct tctgcaacag tgtgacgcat ttttccgccg ggcgcgttcg
      961 cctcagcttg atcttcgcag cgcgggcgca gagattgttg cactgccaca acgaggggaa
     1021 cgtaagatgg ctcgtctctg gaatcgccgc cgcttcgcca agaccgcttt tctggccggg
     1081 gcttcgctcg ccgcctgcct tgccgcggcg tcgccggccc gtgcccagga cgattcgacc
     1141 gcgacgcctc cgggccaggc ggccggcgag gacggcgaga cgatcgtcgt caccgccacc
     1201 aagcgggaga cgacgctgct cgaagtcccc ttctcgatca acgcccagac cgaggaggac
     1261 atccagcgcc tcggcgccgg caatatcgag gacctgtccc gcaacgtcgc cggcctcacc
     1321 gtccagaatc tcgggccggg ccagagccag gtctcgatcc gcggcgtctc cgccggccag
     1381 atcatccgcg accagcccgg cgtcaaggag caggtcggcg tctatctcga cgaatcggtg
     1441 gtctcgctgt cgctgttcca gcccgacctc gacctgttcg acctcaaccg cgtcgagacg
     1501 cttcgcggcc cgcagggcac gctgttcggc tcgggctcgg tcggcggcac catccgctac
     1561 atcaccaacc agccgaccct gtcgcgggtc gagggcgtgg tcgaatccaa cctccatctc
     1621 gtcgaggacg gcgatttcgg cggccacatc aagggcgcga tcaacatccc ggcgggcgat
     1681 catgccgcgg ttcgggtggt cgcctaccac accgaatatg ccggcttcgt cgacgcccgc
     1741 cacgaggccg gaatcgccga cgacgtcaac gacggccggc gcaccggcgg ccgcatcgcc
     1801 gtccgcatcg agccgtcgga ccggatcacg gtcactccgc ggctggtcta tcaggaggtc
     1861 cgctcgcacg gcttcaaccg gcaggaggtc tacaacctct acgccaaccc cttcaccacg
     1921 acccggccgg cggtccagct cggggagcgg gagcagttcc ttcggctgag ggagaggttc
     1981 tcggacaaca ccctgctcgc cgacctcacc gcaagcgccg acctcggcgg catcgagctc
     2041 acctcggtga ccagctacat caaccgcgac atcctggtca gccgcgacgc cagcgccctc
     2101 accggcagcg tctccgtcga tctcggcttc ccggccgggg gcgtcgagct gccgtccaac
     2161 ctggtcgaca cgaccgatct caagaccttc acccaggaac ttcggctttc ctcgaacaac
     2221 gaggggccgt tccagtggct gatcggcgcc ttctattccg acgtcgaccg aatctacgcg
     2281 cagcggctgc cgacgcccgg ctacgacgcg ttcatcaacg cccgcttcgg ccccggcacc
     2341 gccgccgccg ttgccaacgg cttcccggcc aactcgccct acaatgccga ccttccctac
     2401 gacatccagc agaaggccct gttcggcgag gcgagctacg acatcaccga ggctctgacc
     2461 gcgaccgcgg ggctgcgcta ctacgacttc aaggaggtcc gcagcttccg ctcgggcggc
     2521 ctcttctcca acggcgacaa ccagaccgac gagaccaagt ccgacggtct ctcgccgcgg
     2581 ctgctgctca gctacgaggc gtcggacaac gtcatcatca acctgcaggc ctcgaagggc
     2641 ttccgcctgg gcggggtcaa cgatccgctc aacgaagggc tgtgcagcgc ccaggacgtg
     2701 atcgattatg gcggcttcga cacctatgac gacgagagcc tgtggaacta cgaggccggg
     2761 gtgaagtcgc aattcggccc gatcagcgtc aacgccgcgg ctttctacac cgacatcagc
     2821 aacctccagg tcaccgccga cgcgggctcc tgctcgtcgc gagtcgtgtt caacgccgac
     2881 gcccatacca tgggcctcga gttcgagctc tcggcgcggc ccgtccaggg tctcaacctg
     2941 tcgctcgccg gcagcctcgt cgaggcggag ttcgacgaga cgaggctgaa gcagaacggc
     3001 caggtcctgg aagggattcg cgacggcaac aggctgcctt cggtgcccaa gttccagctc
     3061 gccgccagcg ctttctacac cttcccgatc ggcgagggcg aggacgaggc ctatttcggc
     3121 gccaccttcc agcatgtcgg gacgcgcttc acccagccgg gcgaccagga gaacaacccg
     3181 cgcaccttcg tccacggcct gcccttcggc ggggcgccgg cgagcgcgtc gaccacgctc
     3241 gacctgaagc tgcccgacta tcagctggtc aatgtcagcg ccggcatcga gatggcgaac
     3301 ggcttcgacc tcagcgtcta cgtgaacaat ctgttcgacg agaacgcgat cctggccttc
     3361 gaccgcgagc gcggcgggcg ggcgcggctc ggcttccaca tcggccagcc gcgcaccttc
     3421 ggagtgaccg cccgcaagcg gttctgaggg cggccggaac gaggatcagg gacgggcccg
     3481 gagcgcgtcc ctgatctcgc gaagcacgac cagctcctcg ctcggcccgg ccggcgccgc
     3541 ctccggcgtc cgctggaagc gcgaggcggc cttgaccagc tggtagatga cgaaggcgag
     3601 gatcaggaag gcgaccaccg cggtggcgaa ctggccgtag ccgatcatcg gcacgccggc
     3661 cgccttgagc gcggcataat tgttcggatc gcccgcatag tcggcgggga tcgggccgag
     3721 gcgcacgaag tagctcgaga aatcgaggcc gccgaacagc cagccgatca ccggcatgat
     3781 gatgtcgctg gtcagcgagg tgacgatggc ggcgaaggcg gcgccgatga tcacggcgac
     3841 ggccaggtcg accacgttgc ccttggcgat gaattccctg aatcccgaca tgtcggcctc
     3901 ctgtgttgaa gcggggggaa gctagaccgg ttcgcgcaag ccgtcacccc ggcggaagcc
     3961 gggggctcga cgaggcccgc tcgccacggc tttcggtcct cgaggtccgg gctcccgctg
     4021 ggacgacgga agggtcagtg cccggcgtgc gggccttcgt cggccgcgcc gcgcacctcc
     4081 gcatcgaggg tgatcggcgg ggcgacgttg aaggagaagg tgagcggcac tcggtcgccg
     4141 accttcacct tggggctcag tccgaagagc atcgcgtgcc ggccgcccgg ctcgaaggcc
     4201 agctcgccgc ggctcggaaa ctcgatctcc ttgtggcgct tcattttggc gctggtgccc
     4261 gacatgctcg tctcgtgcag ctcgatccag cgcgccgccg ggctcgacac gccgaccagc
     4321 cgggtcccct cgctgcccgc ctccattcgg aaataggcgc tgcctggacg gcttttcgcc
     4381 gccggcagcc ggatccaggc gtctctgacc ctcacggtgc cgtcgggcgg cggccggtcg
     4441 caaccggcga gcagcacggc cccggccagg gccgcgaaga tgtggctttc tagcatcttc
     4501 ctcatcgccg cgctcccgta gccgcgcgcc gacttcttgt cgcctcggaa tcggctccta
     4561 tatcgctccc gaaacactcc gcgtgcctcg ctcgaaggac gcagggggtg cggtaaactt
     4621 ttggggcaca agaggattaa catggccaga gtcatcggaa tcgacctcgg aaccaccaac
     4681 agctgcgtcg ccgtcatgga aggcggcaag cccaaggtgg tcgagaatgc ggaaggcgcc
     4741 cgcaccaccc cctcgatcgt cgccttcgcg aaggacggcg agcggctggt cggccagccg
     4801 gccaagcgcc aggcggtcac caatcccgag accaccgtgt tcgcggtgaa gcgcctcatc
     4861 ggccgccgct tcgacgatcc gatcaccaag aaggacaccg agctggtccc ctacaagatc
     4921 acccgcggcc ccaacggcga cgcctgggtc aatgccggcg ccaaggacta cagcccgtcg
     4981 cagatctcgg ccttcatcct ccagaagatg aaggagacgg cggaaggcta tctcggcgag
     5041 accgtcaccc aggcggtgat caccgtcccg gcctatttca acgacgccca gcgccaggcg
     5101 accaaggatg ccggccagat cgccggcctt gaagtgctgc gcatcatcaa cgagccgacc
     5161 gcggccgcgc tcgcctacgg gctcgacaag caggacggca agacgatcgc ggtctacgac
     5221 ctcggcggcg gaacgttcga catctcggtg ctcgagatcg gcgacggcgt cttcgaggtg
     5281 aagtcgacca acggcgacac cttcctcggc ggcgaggatt tcgacgcgaa gatcgtcgac
     5341 ttcctggccg acgagttcaa gaagcaggag ggcatcgacc tcaggagcga ccggctcgcg
     5401 ctgcagcgcc tcaaggaggc cgcggagcgg gcgaagatcg agctcagctc ggcccagacc
     5461 accgaggtca acctgccctt catcaccgcc gaccagaacg gccccaagca tctcgtcaag
     5521 gcgatcagcc gcgccgacct ggagcgtctg gtcgaggacc tgatcaagcg cacgctcgag
     5581 ccgtgcaaga aggcgctggc cgatgccggg gtcaaggcgg aggacatcgc cgaggtggtc
     5641 ctcgtcggcg gcatgacccg gatgccgcgc gtgcgtcagg tcgtccagga cttcttcggc
     5701 aaggagccgc acacgggcgt caaccccgac gaggtcgtcg ccatgggcgc cgcgatccag
     5761 gccggcgtcc tccagggcga cgtcaaggac gtcctgctgc tcgacgtgac gccgctgtcg
     5821 ctgggcatcg agactctggg tggggtgttc acccgcatga tcgaccgcaa cacgaccatc
     5881 ccgaccaaga agagccagac cttctcgacc gccgaggaca atcagaacgc ggtcaccatc
     5941 cgggtcttcc agggcgagcg ggagatggcg atcgacaaca agatcctcgg ccagttcgac
     6001 ctggtcggaa tcccgccggc gccgcgcggc gtgcctcaga tcgaggtcac cttcgacatc
     6061 gacgcgaacg gcatcgtcca cgtctcggcc aaggacaagg gcacgggcaa ggagcagcag
     6121 atccggatcc aggcctcggg cggcctttcc gacgccgaca tcgacaagat ggtccgcgag
     6181 gcggagagct tcgccgagga ggacaagaag cgccgagccg cggccgaggc gaagaacagc
     6241 gccgagggcc tcgcccattc gaccgagcgc cagctcgccg agcatggcga caagatcgac
     6301 gaggcgctga aggccgagat ccagaccgcg atcgacgaga cgaagcaggc ggtggagagc
     6361 ggcgacgtcg aggccatgca ggccaagacc tcggcgctca gccaggtcgc gatgaagctc
     6421 ggccaggcga tctacgagaa ggagcaggcc aatgccggcg gctccggcgg cgacaccgaa
     6481 gccgcgcagg gcgccgagcc cggcggcggc gaggaagagg tcgtcgacgc cgaattctcc
     6541 gaggtcgacg acgagaacaa gggctgagtc atggatttcc gtcattccag cggaagctgg
     6601 aatccatgcg gcatcgccgt cctagccggg aatggagagc cagcatggac cccagcctgc
     6661 gctggggtga cggcatccgg ccggggggcc gtctaccgtc atggctgagc aggattatta
     6721 cgagctgctg caggtcgagc ggggcgccga cgagcggacc ttgaagtccg cctatcgccg
     6781 cctcgccatg gaatgccatc cggaccgcaa ccccggctgc aaggattccg aggcgcggtt
     6841 caaggctatc aacgaggcct atgactgcct caaggacccg cagaagcgcg ccgcctacga
     6901 tcgcttcggc cacgccgcct tccggaacgg cggcatgggc ggggcgggcg cccaggattt
     6961 cggctccttc tccgacatat tcgagaacat cttcggcgag ttcatgaacg gccagcaggc
     7021 cgaccgccgc tcgaacgcgc tgcgcggctc ggacctgcgc tacgacctcg agatcagcct
     7081 cgaggacgcc tatgccggca agcaggtcga gctgcgcatc gataccaccg cccgatgcga
     7141 gccctgcggc ggctccggag ccaaggccgg cacccaggcg cggacctgca ccatgtgcca
     7201 cggccggggc caggtgcgcg ccagccaggg cttcttcgtg gtcgaacgga cctgcccgac
     7261 ctgccgcggc gtcggcgagg tcatcgccga cccgtgcgag cgctgccgcg gcgaggggcg
     7321 gatcgagcgc cagcgctcgc tcgacgtgcg cattccggcc ggagtcgacc aaggcacccg
     7381 catccggctc gccggcgagg gcgaggccgg ggtgcgcggc gggcccaacg gcgatctcta
     7441 catcttcgtc catctggcgc ggcatccggt cttccagcgc gaggggacga cgctgttcgt
     7501 ccgggcgccg gtcagcatca ccaccgccgc gctcggaggc tgcatcgagg tccccgggct
     7561 cgaccggcag aagcattcga tcgccatccc ggccggaatc cagtcgggca agcagctgcg
     7621 gcagcgcggc gccggaatgc cggtgctcaa cggccgcggc cacggcgaca tggtcatcca
     7681 ggtcgaggtc gagacgccga cccgcctgac cgcgcggcag aaggaattgc tcgaggcctt
     7741 ccgcgaaacc gagaccggcg aggaatgccc ggcgtcgaag agcttcttcg gcaagctgaa
     7801 ggggctgttc gacgcctgat gtcccgcccc cgcgaagcgg gtggtcgtgc ggtgtccttc
     7861 gatacgggcc ttcgccacgc tcggtcccgg ctcaggatga gcggctggga ttgtcgctca
     7921 tcctgagtag ccgctgggcg aagtcgaagc ggcgtatcga aggaccgctt cgcatgggga
     7981 ggacaagggg gattcgcggc gctaaggtcg cgcgatgcgc ttcctgctcc cgctcctcgc
     8041 cctggttgcc gcctcctgcg ccaccgctcg ccaaacgccg cccaccttcg ccaatcccgt
     8101 catcgactcg gacttccccg atccggcgat cctgcgcgcc tcggacggct tctactacgt
     8161 ctacgccacc cagacggagc gcgacggcaa gtgggtgaac ctccaggtcg cccgctcccg
     8221 cgacctcgtc gcctgggagc atctgggcga cgctttgccc gccaagccgg ggtgggcgag
     8281 caagacgcag gacttctggg cgccccacgt cgccgagcat ggcggccgct actatctcta
     8341 ttattcggcc aagccggacg aggcgctgat cgacgacaag aaggggctgt gcctggcggc
     8401 cgcgaccgcc gaccgtccgg aagggccctt caccgacatg ggccggccgc tcctgtgcgg
     8461 cgcgagcttc gtcaacatcg atccgatgag ctatgacgat cccgccaccg gcaggcggct
     8521 gctctactgg ggctcgggct tccagccgat caaggtccag gagctggcgc cggaccgcct
     8581 gtccttcgcg ccgggaagcg ctccgatcga tctcgtcccg gccgatccga agacggacta
     8641 ccgcaagctc gtcgaaggcg cctggatcgt gcttcgcggc ggttattact acctcttcta
     8701 ttccggcgac aattgctgcg gcccgggcgt gcattacgcg gtgatggtcg cccgctcgcg
     8761 aagcgccacc ggcccgttcg agctgcagcc gggcaccctc ctcgcgcaga gcccgaaatg
     8821 gatcggcccg ggccacaaca gcgtcgtcga ggacgatgat ggcgagagct ggatcgtcta
     8881 ccatgctgtc gattccgccc ggccccgcac caagccgacc gacgaggtca acacccggcg
     8941 ggtcatgctg atcgaccatc tcgagtggac cgacgaatgg ccgcgggtga aaggaccgac
     9001 cagcggacct cagcgccgcc gctatccgga gcgttacgcg ccggactgag acgccgtcga
     9061 aaaggggaaa tccatgcgcc ttcctgcctt cgccctagcc gctctgctgg ctgcgcccgc
     9121 cttcgcgcag caggacctct ccaaggtcga agtcaaggtc gagaggatcg cgccgggggt
     9181 cgcggtcctg ctcggagcgg gaggcaatat cggcctctcc tacggcgcgg acggcaacat
     9241 cgtcgtcgac gaccaatatg cgccgatgag cgagaagatc ctggccgcga tcgcgaccgt
     9301 cgatccggat ccagtccgct tcgtcgtcaa cacccactgg cacggcgacc ataccggcgg
     9361 caacgaggcg atgggcaagc gcggggccgt catcgtcgcc cacagcaacg tgcgggagcg
     9421 gatgtcgagc gagcagttca ttgcggccct cgacgaccgg gtgcccgcct cgccgccggc
     9481 cgcgctcccg gtggtgacgt tcgaggacgg ggtcgcgctc cacctcaacg gcgacacgct
     9541 ccacgtcgtc cacgtcgcca acgcccatac ggacggcgat tcgctggtcc actggcagaa
     9601 ggcgaacgtg ctccacatgg gcgacacctt cttccacaaa ttctcgctgc ccttcatcga
     9661 cctgagctcg ggcggctcga tcgacgggct gatcgcggcc gtcgacaagg ggctcgcgtg
     9721 gagcaacccg gcgacccgga tcatccccgg ccacggcccg gtcgcctcca gggcggagct
     9781 cgccgcctac aaggcgatgc tggtcgacgt ccgggccaag gtggcggccg gaatccgcgc
     9841 caggcgcagc ctcgcccaga tccaggcgac gaagccggcg gcgccctatg ggatggcgga
     9901 cggcttcatc aagcccgacc agttcgtcgc cttcgtccac gagagcctga ccaagccggc
     9961 gaaggcgggc cacgcccacg gcggcgagac ccacagccac tgagcgccgg ctcagcggcg
    10021 cgtcagcagg tcctcgacga cgctgagctt cgtctcctcg acgtggagaa tgccgggcca
    10081 gggctcgagc ggcccgcgcg cggcgaaggc cgccgctcgc gcctgctcgt ccggccagcg
    10141 ggcgaaggcg atgaagtcgc cattctccgc ccgggtgagg caggagccga gggcgccgtg
    10201 ctcgtccct
//
LOCUS       DASVQZ010000318         8279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_262182, whole genome shotgun sequence.
ACCESSION   DASVQZ010000318 DASVQZ010000000
VERSION     DASVQZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262182"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VF547_08820"
     CDS             <1..488
                     /locus_tag="VF547_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262962.1"
                     /translation="LGLSPNGDLIVGDAPGHRVLKVAPDGTLSVLIGPDKGIAKPESF
                     AFDDRGNLYIADNRDNVLYLLTADGILHRAIADRRGFSPESLRFAGGALFITDSDHGK
                     LFRYTPEDGLTVVALFGGTLANVQGIAADGRGTLYLSVQSDLAARKGYILKLYRRGGR
                     V"
     gene            498..1346
                     /locus_tag="VF547_08825"
     CDS             498..1346
                     /locus_tag="VF547_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8262963.1"
                     /translation="MSQDAPFDRRLRRLRRERAAKRFHQADYLHRTMADELIERLGLV
                     RRDFRRALDLGCAGGHLTRRLRELGLEVTACDAGAAFARSAGGVQADEDRLPFADGAF
                     DLVVSAGALDGVNDLPGALILIRRVLRPDGLFLGAFAGAGSLPRLRRAMLAADSVEGG
                     AAPRVHPQIDVRAAGDLLSRAGFALPVVDVEPVDVRFGAFLDLVRDLRAMAATNILAE
                     RSRRPLGRRALAAAIADFEQGGGRTAERFEILHLLGWAPSPDQPRPARRGSATASLAE
                     ALKRRD"
     gene            complement(1487..1894)
                     /locus_tag="VF547_08830"
     CDS             complement(1487..1894)
                     /locus_tag="VF547_08830"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX8262964.1"
                     /translation="MEKIPTLTVVAAALVDSERRVLLQRRAPGRAMAGLWEFPGGKVE
                     PGERPEAALARELEEELGIGVETDSLRPAAFASADNAGRHMLLLLYLCREWRGEPKAL
                     DAAELAWVRPADMAGLPMPPADEPLIPLLDRLV"
     gene            1982..2155
                     /locus_tag="VF547_08835"
     CDS             1982..2155
                     /locus_tag="VF547_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8262965.1"
                     /translation="MRLFAKLIRDTKAASAVEYALICALIVLAMIAGLNAVAGSTTTM
                     WNEVSNNVVSTSN"
     gene            2288..2455
                     /locus_tag="VF547_08840"
     CDS             2288..2455
                     /locus_tag="VF547_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX8262966.1"
                     /translation="MNFIRKIRKSEKGATAIEYGLIAALIAVAAIAAMQNIGASLGTT
                     FNTVANDMSAA"
     gene            complement(2565..4034)
                     /locus_tag="VF547_08845"
     CDS             complement(2565..4034)
                     /locus_tag="VF547_08845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8262967.1"
                     /translation="MKMKALFALSVSLAAVVSVGDGAPASAQGRTRSISQSTAATAAK
                     QHPQIVAEFGGEEGGARSAYIREVGARVAAQTNIAGGGNAFRVTTLNSPVMNAFAVPG
                     GYLYVTRQLVGLANDEAELASVLGHEAGHIAARHSSERKRAGIFSQLLAVGLGVVTGS
                     TQLASLAGQVTQGLVLGYSRNQELEADDLGVAYIAAAGYDPAASASFLNSLGAATSLE
                     ARAVGRNDERAVPSWARTHPLSADRVRRATQKAQATGRSGTGLRNRDAFLAQIDGIMV
                     DDDPRQGVIDGRTFTHPYLRLRFAVPEGYGIQNGTKAVSIAGQRGQAQFSGGAFSGDL
                     GAYLDQAFRAIAGQNARLGYVEPRSTTINGLPAAWSTARVNTQSGQVDLTIVAYRWDS
                     DTAYHFAMITPAGSGLGPFAPMVESLGRISAAEAAAIRPRIIDVVTVGPRDSVATLAG
                     RMAYDDHKEERFRVLNGLAPGAALKPGQKVKIVVYGTRV"
     gene            complement(4133..5074)
                     /locus_tag="VF547_08850"
     CDS             complement(4133..5074)
                     /locus_tag="VF547_08850"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX8262968.1"
                     /translation="MPTFLDFEKAIAELESRIEELRETAEGSDLNIDSEIGRLEAKSD
                     RMLRETYGRLTPWQKTQVARHPDRPHFKDYVAALVEDFMPLAGDRAFGEDAAIVGGLG
                     RLGGRKIVLIGHEKGDDTASRLTHNFGMAKPEGYRKAIRLMELADRFALPVVTLVDTA
                     GAFPGVQAEERGQAEAIARSTEACLALGVPMVAAIVGEGGSGGAIAIAAANRVLMLEH
                     SVYAVISPEGCASILWRTAEKAADAAEAMRVTAQDLKALGVIDRIIPEPVGGAHRRPS
                     EAIAALGDALGEELDALARLSPGEVRADRRAKYLAIG"
     gene            complement(5089..5994)
                     /locus_tag="VF547_08855"
     CDS             complement(5089..5994)
                     /locus_tag="VF547_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8262969.1"
                     /translation="MSCAREDGQLIAAFLEMLAAEAGAALNTRLAYERDLRGASELLG
                     GRLSGASREELNRLSQAWLPLKRTTVARKAAALRRFYGFLHDEGLRSDDPSAALPRPG
                     GSRPLPRILDHEAVAALFAEIERRRREAPGDATLRLAALVELLYGSGLRATELVSLAR
                     HAVHPDRPFLILRGKGAKERLVPISDRARAAVAEWAATVPRDSLWLFPSGRSHLSRVR
                     LHQLLKELAAAAGVPPERVSPHVLRHAFATHLLEGGADLRALQLLLGHADIATTQIYT
                     HVDSRRLIDLVNSRHPLVDAKPPRA"
     gene            complement(5991..7772)
                     /locus_tag="VF547_08860"
     CDS             complement(5991..7772)
                     /locus_tag="VF547_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262970.1"
                     /translation="MKPWRARLLLLAGGAALAFAIPAAGQRDTGPESLLPPGFGEPPP
                     PAPKEPAPDAPPTPSRPSAPAPLLPPSSAEALPVEDSAAEDLEALAQQALPPPIEIPE
                     ASRRPTNPVGPLRPDNWGLGLDAFGNANGRFLGGLMGRLNAPLPSRWASILLRRALLS
                     EASAPPLVNEVDWIAERAWLLLRMGEADSARMLVQAVDVDRFTPRMFTVAVQVALATA
                     DPAALCPLVGPGRAISDQPVWPLADAMCASLEGDAALASQLIEQARRRSGGGIDTLLA
                     EKVIGAGANTRRAVTIEWAGVEELSSWRFGLASATGLEIPAELMNRAGPAVWAWQARA
                     PMIPIEQRVAAAQRAATLGVFSNASLVEMHSLIADQTDPSEVEGSVGDRLRRAYAAGS
                     IGDRIDAMRNLWGEGDDRYGRFILTAAAAARVPPASEHGNRTDDLLGSMMTAGYDRFA
                     ARWGGVADALDGDDGHRAWSILAVGAPRPVVDLGAGRVEAFTDGVDAHRGRMLVAALA
                     GLGRLADPAAYGVDPAPRSRWANLIAAAARAGQPGTVALLAGVGMQTPSWRGVPPEHL
                     YHVVRALRAVGHEYEARMIAAEAMTRL"
     gene            complement(7753..7920)
                     /locus_tag="VF547_08865"
     CDS             complement(7753..7920)
                     /locus_tag="VF547_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262971.1"
                     /translation="MRISPPPRRRKSVLPKLLIALLLLFLGLLVWLGMRDTEVPTQRI
                     EQDVTNEALAG"
     gene            7969..>8279
                     /locus_tag="VF547_08870"
     CDS             7969..>8279
                     /locus_tag="VF547_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX8262972.1"
                     /translation="MADALPRPEVPIVLVGLMGVGKSTVGRRLARRLGLAFVDSDEEI
                     ERAAVHTIPEIFDRFGEASFRDGERRVLRRLIEGGPKVVATGGGAFMDDETRALILER
                     CL"
BASE COUNT         1172 a         2884 c         3078 g         1145 t
ORIGIN      
        1 ggctgggcct ctccccgaac ggcgacctca tcgtcggcga cgcgcccgga caccgggtgc
       61 tgaaggtcgc gccggacggc acgctgtcgg tgctgatcgg gcccgacaag ggcatcgcca
      121 agcccgagag cttcgccttc gacgaccgcg gcaacctcta catcgccgac aatcgcgaca
      181 acgtcctcta tcttctgacc gcggacggga tcctgcatcg ggcgatcgcc gaccgccgcg
      241 gcttctcgcc ggaatcgctc cgcttcgccg gcggagcgct gttcatcacc gacagcgacc
      301 acggcaagct gttccgctac acgccggagg acggactcac cgtcgtcgcg ctgttcggcg
      361 ggacgctcgc caacgtccag gggatcgccg ccgacgggcg cggcaccctc tatctctcgg
      421 tccagagcga tctcgccgcg cgcaagggct atatcctcaa gctttatcgc cggggcggcc
      481 gcgtctaacg aggcgcgatg agccaggacg cgcctttcga ccggcggctg cggcggctgc
      541 gccgcgagcg cgcggcgaaa cgcttccacc aggccgacta tctgcaccgg acgatggccg
      601 acgagctgat cgagcggctc ggcctggtca ggcgcgactt ccgccgggcg ctcgatctgg
      661 gctgcgcggg gggccatctg acccgccggc tgcgcgagct cgggctggag gtgacggcgt
      721 gcgacgccgg cgccgccttc gcgcgctccg ccggcggggt ccaggcggac gaggaccgcc
      781 tccccttcgc ggacggcgcc ttcgacctcg tcgtctcggc gggcgcgctc gacggagtca
      841 acgatctgcc cggggcgctg atcctgatcc ggcgcgtgct ccggcccgac ggcctgttcc
      901 tcggcgcctt cgccggcgcc gggagcctgc cgcgcctcag gcgggcgatg ctcgcggccg
      961 attcggtcga aggcggcgcg gcgccgaggg tccatccgca gatcgacgtc cgagccgccg
     1021 gcgacctgct gagccgggcc ggcttcgcac tgccggtggt cgacgtcgag ccggtcgacg
     1081 tccgcttcgg cgccttcctc gacctcgtcc gcgaccttcg cgcgatggcg gcgaccaaca
     1141 tcctcgccga acggtcgcgg cggccgctgg gacggcgcgc gctcgccgcg gcgatcgccg
     1201 atttcgagca gggcggaggc aggacggcgg agcggttcga gatcctccat ctgctgggct
     1261 gggccccttc cccggaccag ccccggccgg cccggcgcgg aagcgccacg gcctcgctcg
     1321 ccgaggcgct gaagcggcgg gactgaacga ttcctccccg cgatttcgcg gggaggggga
     1381 ccaggcgaag cctggtggag gggttctttg agtctccgaa gaacccctcc accgcgcttc
     1441 gcgcggtccc cctcccccgg aaaatccggg ggaggaacaa ggggctctag accagccggt
     1501 cgagcagcgg gatcagcggc tcgtcggcgg gcggcatcgg caggccggcc atgtcggccg
     1561 ggcgcaccca ggccagctcg gcggcgtcga gggccttcgg ctcgccgcgc cactcgcggc
     1621 acaggtagag gagcaggagc atgtgccgcc cggcattgtc ggcgctggcg aaggcggccg
     1681 gccgcagcga atccgtctcg acgccgatgc ccagctcctc ctcgagctcg cgggcgagcg
     1741 cggcctcggg ccgctccccc ggctcgacct tgccgccggg aaactcccag aggccggcca
     1801 tcgcccggcc gggcgcccgg cgctgaagga ggactcggcg ctcggagtcg accagcgccg
     1861 cggcgacgac ggtcagtgtc ggaatttttt ccagattcga ggccagcgga aggactccgt
     1921 taactcagac gacctatcca gagcggatgg gcgaggagcc agacggggga aggtaaggtc
     1981 gatgcgactt tttgcaaagc tgatccgcga taccaaggcg gccagcgccg tcgaatatgc
     2041 cctgatctgc gccctgatcg tgctggcgat gatcgccggc ctcaacgccg tcgccggatc
     2101 gacgacgacg atgtggaacg aggtttccaa caacgtcgtc tcgacctcca actagtcgaa
     2161 ccgacggcca ggggttgata acgatttgga aacttccttg ccctaaatcg gaagggtccg
     2221 cccgcaaagt ggaacagggg gcggctggaa gtgtggactt ccaaagcttt ggaacaggag
     2281 accggaaatg aatttcattc gcaagattcg caagagcgaa aagggcgcca ccgccatcga
     2341 atacggcctg attgccgccc tgatcgccgt cgccgccatc gccgcgatgc agaacatcgg
     2401 cgccagcctc ggcacgacct tcaacaccgt cgccaacgac atgtcggcgg cctaatcgcc
     2461 ttaccagcga tttgaaaggg aggcggcgga acccaggttc cgccgcctct ttcgtcattg
     2521 cgggcacggc gaagcctcgc tccccgcaat gacgaggaaa gggctcagac gcgggttccg
     2581 tagacgacga tcttgacctt ctggccgggc ttcagcgcgg cccccggggc gaggccgttg
     2641 aggacccgga agcgctcctc cttatggtcg tcataggcca tccgcccggc gagggtcgcc
     2701 accgagtcgc gcggtccgac ggtgacgacg tcgatgatcc gcggccggat cgccgccgcc
     2761 tcggccgcgc tgatccggcc cagcgactcg accatcggcg cgaacgggcc gagcccgctt
     2821 cccgcgggcg tgatcatcgc gaaatgatag gcggtgtccg agtcccagcg ataggcgacg
     2881 atggtgaggt cgacctggcc cgactgggtg ttgacccgcg ccgtcgacca ggcggcgggc
     2941 aggccgttga tcgtcgtcga gcgcggctcg acatagccga ggcgcgcgtt ctggccggca
     3001 atggcccgga acgcctggtc gagataggcg ccgagatcgc cgctgaaggc gccgcccgag
     3061 aactgggcct ggcccctctg gccggcgatg ctgaccgcct tggtcccgtt ctggatgccg
     3121 tagccctcgg gcacggcgaa gcgcagcctc agataggggt gggtgaaggt ccggccgtcg
     3181 atcacgccct gcctcggatc gtcgtcgacc atgatcccgt cgatctgcgc gaggaaggcg
     3241 tcgcggttcc tgaggccggt tccggagcgg ccggtcgcct gcgccttctg cgtggcgcgc
     3301 ctgacccggt cggcgctgag cggatgggtc cgggcccagc tcggcaccgc ccgctcgtcg
     3361 ttgcgcccga ccgccctcgc ctcgagcgag gtcgccgcgc ccagcgaatt gaggaagctc
     3421 gccgaggcgg cgggatcgta gccggcggcg gcgatgtagg cgactccgag gtcgtcggcc
     3481 tcgagctcct ggttgcgcga atagccaagc accagcccct gggtgacctg gccggcgagg
     3541 ctggcgagct gggtcgagcc ggtgacgact ccgaggccga cggcgagcag ctggctgaag
     3601 atcccggcgc gcttgcgctc cgagctgtgg cgggcggcga tatggccggc ctcgtggccg
     3661 agcaccgagg cgagctcggc ctcgtcattg gcgagcccga ccagctggcg ggtgacgtag
     3721 agatagccgc ccgggacggc gaaggcgttc atcacgggcg agttcaaggt ggtgacgcgg
     3781 aaggcgttgc cgcctccggc gatgttggtc tgcgcggcga cccgggcgcc gacctccctt
     3841 atgtaggcgc tgcgcgcgcc gccctcctcg ccgccgaact cggcgacgat ctgcggatgc
     3901 tgcttggcgg cggtggccgc cgtgctctgc gagatgctgc gggtcctgcc ctgggccgag
     3961 gccggtgcgc cgtcgccgac cgagacgacg gcggcgagcg agacggacag cgcgaaaagc
     4021 gccttcatct tcatcgctgg atactcctca cactggacga ttcgtcgccg taaggaacgg
     4081 ggcaagggga gcgaggttcc ttttgcgccc catgcggagc acgggaggcg ggtcagccta
     4141 tcgcgagata tttcgcccgg cggtcggcgc ggacttcgcc gggcgagagc cgcgccaggg
     4201 cgtccagctc ctcgccgagc gcgtcgccga gggcggcgat cgcctcggag gggcggcggt
     4261 gggcgccgcc gacgggctcg gggatgatcc ggtcgatgac tccgagcgcc ttgagatcct
     4321 gcgccgtcac ccgcatcgcc tcggcggcgt cggccgcctt ctcggcggtc cgccacagga
     4381 tcgaggcgca gccttcgggc gagatcacgg cgtagacgct gtgctcgagc atcagcaccc
     4441 ggttggccgc ggcgatggcg atggccccgc ccgagccccc ttccccgacg atcgccgcga
     4501 ccatcggcac gcccaaggcg aggcaggcct cggtcgagcg cgcgatcgcc tccgcctggc
     4561 cgcgctcctc ggcctgcacg ccgggaaagg cgccggcggt gtcgaccaag gtcaccaccg
     4621 gcaaggcgaa gcggtccgcc agctccatca ggcggatcgc cttccgatag ccttccggct
     4681 tggccattcc gaaattgtgg gtgaggcggc tggcggtgtc gtcgcccttc tcgtggccga
     4741 tcaggacgat cttgcggccg cccagccggc cgaggccgcc gacgatcgcc gcgtcctcgc
     4801 cgaacgctcg gtcgccggcg agcggcatga aatcctcgac cagcgccgcc acatagtcct
     4861 tgaagtgggg gcggtccgga tggcgggcga cctgggtctt ctgccacggc gtcagccggc
     4921 cgtaggtctc gcgcagcatc cggtcggact tggcctcgag ccggccgatc tcggagtcga
     4981 tgttgaggtc ggacccctcg gccgtctcgc gcagctcctc gatgcgcgat tcgagctcgg
     5041 ctatggcctt ttcgaagtcg aggaaggtgg gcatgtccgg gcgcggggtt aagcgcgcgg
     5101 cggctttgcg tcaacgagcg ggtggcggct gttgacgagg tcgatcaggc gccggctgtc
     5161 gacatgggtg tagatctgcg tggtggcgat gtcggcgtgg ccgaggagga gctggagggc
     5221 gcgaaggtcg gcgccgcctt cgagcagatg ggtggcgaag gcgtggcgca ggacgtgggg
     5281 gctgacccgc tcgggcggca ccccggcggc cgcggcgagc tccttcagca gctggtggag
     5341 gcgcacccgg ctgaggtgcg acctgcccga agggaacagc cagaggctgt cgcgcggcac
     5401 cgtcgccgcc cactccgcga cggcggcacg ggcgcggtcc gagatcggca ccagccgctc
     5461 cttcgccccc ttgccgcgaa ggatcaggaa cggccggtcg gggtggacgg catggcgcgc
     5521 aagcgagacc agctcggtcg cccgcagccc ggagccgtag agcagctcga ccagggcggc
     5581 gaggcggagc gtggcgtcgc ccggcgcctc gcggcggcgg cgctcgatct cggcgaacag
     5641 cgccgccacc gcctcatggt cgaggatcct gggaagcggc cgcgacccgc ccggccgcgg
     5701 cagcgccgcc gacgggtcgt cggaccgaag cccttcgtcg tgcaggaagc cgtagaagcg
     5761 gcggagcgcc gcggccttgc gcgcgaccgt ggtccgcttg agcggaagcc aggcctggga
     5821 gaggcggttc aactcctccc tcgaggcgcc cgagagccgg ccgccgagca gctcggacgc
     5881 gccgcgcagg tcgcgctcgt aggcgaggcg ggtgttgagc gcggctcccg cctcggcggc
     5941 cagcatctcg aggaaggcgg caatcagctg cccgtcctct cgggcgcagc tcacaggcgg
     6001 gtcatcgcct cggcggcgat catccgcgcc tcatattcgt ggccgacggc cctcagcgcc
     6061 cgaacgacgt ggtagaggtg ctcgggcgga acgccgcgcc agctcggagt ctgcatgccg
     6121 accccggcga ggagggcgac ggtgccgggc tggccggccc gcgccgccgc cgcgatcaga
     6181 ttggcccagc ggctgcgcgg cgccggatcg actccatagg cggcgggatc ggcgagtcgg
     6241 ccgaggccgg cgagggcggc gaccagcatc cgcccgcgat gcgcgtcgac gccgtcggtg
     6301 aaggcctcga cccggccggc gccgagatcg acgaccggcc gcggcgctcc gaccgccagg
     6361 atcgaccagg cccggtgacc gtcgtcgccg tccagggcgt cggcgactcc gccccagcgg
     6421 gcggcgaagc ggtcgtagcc ggcggtcatc atcgatccga gcaggtcgtc ggtgcggttg
     6481 ccgtgctcgg aggcgggcgg aacccgcgcg gcggcggcgg cggtgaggat gaagcggccg
     6541 tagcggtcgt cgccttcgcc ccacaggttg cgcatcgcgt cgatccggtc gccgatcgag
     6601 cccgccgcat aggcccggcg caggcggtcg ccgacgctgc cctcgacctc ggacggatcg
     6661 gtctggtcgg cgatcaggct gtgcatctcg accagcgagg cgttggagaa gaccccgaga
     6721 gtcgccgccc gctgcgccgc cgccacgcgc tgctcgatcg ggatcatcgg cgcgcgcgcc
     6781 tgccaggccc agacggcggg gccggctcgg ttcatcagct cggccgggat ctcgaggccg
     6841 gtggcgctgg cgaggccgaa gcgccagctg ctcaattcct ccaccccagc ccattcgatg
     6901 gtgacggcac ggcgggtatt ggcgccggcc ccgatcacct tctcggcgag cagggtgtcg
     6961 atgccgccgc cgctgcgccg ccgggcctgt tcgatcagct ggctggccag cgcggcgtcg
     7021 ccctccaggg aagcgcacat cgcgtcggcc agcggccaga cgggctggtc ggaaatggcg
     7081 cgcccgggac cgacgagcgg gcagagcgcg gcgggatccg cggtggcgag cgcgacctgg
     7141 acggcgacgg tgaacatgcg cggcgtgaag cggtcgacgt cgaccgcctg gaccagcatc
     7201 cgcgcgctgt ccgcctcgcc catgcgcagc agcagccagg cgcgctcggc gatccagtcg
     7261 acctcgttca ccagcggcgg cgccgacgct tccgagagca aggcgcggcg cagcaggatc
     7321 gaggcccagc gcgagggcag cggcgcgttg aggcggccca tcaggcctcc caggaaacgg
     7381 ccgttggcgt tcccgaacgc atcgaggccg aggccccaat tgtcgggccg caaggggccc
     7441 accgggttgg tcgggcgccg cgacgcctcg gggatctcga tcggcggcgg cagcgcctgc
     7501 tgggccagcg cctcgagatc ctcggcggcc gaatcctcga ccggcagcgc ctcggccgac
     7561 gacgggggca ggagcggggc cggggcggaa ggccgcgacg gggtcggcgg ggcgtcgggc
     7621 gccggctcct tgggcgcggg cggcggcggc tcgccgaagc cgggcggcag cagcgattcg
     7681 ggacccgtgt cgcgctggcc cgcggccggg atggcgaagg ccagagccgc gccgccggcg
     7741 agaaggagga gcctagcccg ccagggcttc attggtgacg tcctgttcga tccgctgggt
     7801 cggcacctcg gtgtcgcgca tgcccagcca cacgagcagc ccgaggaaga ggaggagaag
     7861 ggcgatgagc agcttcggca ggaccgattt acgccggcgc ggcgggggtg agatacgcat
     7921 ggccggcctt ttgccccacg agccggcccc ctgtatagcc cctgccgcat ggccgacgcg
     7981 cttccccgac ccgaggtccc gatcgtcctg gtcggactga tgggcgtcgg caagtcgacg
     8041 gtcgggcgcc ggctggcgcg ccggctcggc ctcgccttcg tcgattcgga cgaggaaatc
     8101 gagcgcgccg cggtccacac catccccgag atcttcgacc gcttcgggga ggcgagcttc
     8161 cgggacgggg aacggcgggt gcttcggcgg ctgatcgagg gagggccgaa ggtggtcgcg
     8221 accggcggcg gcgcgttcat ggacgacgag actcgagcgc tgatcctcga gcggtgcct
//
LOCUS       DASVQZ010000319         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_546559, whole genome shotgun sequence.
ACCESSION   DASVQZ010000319 DASVQZ010000000
VERSION     DASVQZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546559"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="VF547_08875"
     CDS             complement(<1..1936)
                     /locus_tag="VF547_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8262973.1"
                     /translation="MAGEDMYAMSEERSSGAWRGVLASAGAVLAALLLVVLMLMVTSS
                     NRVRDEALASERHSYDVMLLTRTVDGTIARAEAALGRYVLDEDVATSGSVYGTEWRQA
                     RQLIGELRRQVRSNPEQLARVEALERLFEARGRELAPAALAAQAKRGSGGISLFYGAA
                     RAPTGQQMRNLLAQIATAERDDLRNRMNATRLSGTRSDQLTKWLGWLAVAIGVGAIVL
                     GWLAFRAISERLEALREADSEANRARALEEAVLERTSELSQAYEQLKAQSAEREAAEA
                     QLRQVQKMEAVGQLTGGIAHDFNNMLAVVVGGLDLARRKLTGPRREVEFHLDNALEGA
                     TRAAALTRRLLAFARAEPLLPVAVAPAGLVEGMLELVDRSIGERITVRTRFPDEPWNV
                     CVDTNQLENAILNLAVNARDAMDGEGQLAISVENAPLEAGRIGALPAGDYVRISVSDT
                     GSGMPREVLERVFEPFFTTKPVGKGTGLGLSQIFGFARESGGDVVIDSTPGVGTTVSL
                     YLPRSLEPVETPAAQAAAEDEAPVETGTAILLVEDDPRVSRSTVAALEELGYRPIACA
                     GGREALDLLEREQGRIELVITDVMMPEMTGPELVAAATERWPSLAILYVTGYVGEAGS
                     EQLSGHEILRKPFTVAGLAGA"
     gene            complement(2042..2368)
                     /locus_tag="VF547_08880"
     CDS             complement(2042..2368)
                     /locus_tag="VF547_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8262974.1"
                     /translation="MAAPLALPPEIHPPAPIGPYIADLACRRAKLVVEIDGSQHIGSR
                     RDDVRTRFLEAAGWTVIRFWNSDVGTNPDGVAEAILQRAAECLGGTHPQPLPSREGRQ
                     RRRRFG"
BASE COUNT          322 a          840 c          881 g          355 t
ORIGIN      
        1 cggcgccggc cagccccgcg accgtgaacg gcttcctcaa tatctcgtgg ccgctcagct
       61 gctccgagcc ggcctcgccg acatagccgg tgacgtagag gatggcgagc gagggccagc
      121 gctcggtggc ggccgccacc agctccggac cggtcatctc cggcatcatc acgtcggtga
      181 tgaccagctc gatccggccc tgctcgcgct cgagcaggtc gagcgcctcg cgcccgccgg
      241 cgcaggcgat cggccggtag ccgagctcct cgagcgccgc gacggtcgag cggctgaccc
      301 tcggatcgtc ctccaccagc aggatcgcgg ttccggtctc gaccggcgct tcgtcctcgg
      361 cggccgcctg cgcggccggg gtttccaccg gctcgagcga gcgcggcaga tagagggaga
      421 cggtggttcc gacgccgggg gtcgaatcga tcacgacgtc gccgcccgat tcgcgcgcga
      481 agccgaagat ctggctgagg ccgaggccgg tgcccttgcc caccggcttg gtcgtgaaga
      541 agggctcgaa cacgcgctcc agcacctcgc gcggcatgcc cgagccggtg tcggagacgc
      601 tgatccggac atagtcgccg gccggcagcg ccccgatccg gccggcctcg agcggggcgt
      661 tctcgacgct gatcgcgagc tggccctcgc cgtccatcgc gtcgcgcgcg ttgacggcga
      721 ggttgaggat ggcgttctcc agctggttgg tgtcgacgca cacgttccag ggctcgtcgg
      781 ggaagcgggt gcgcacggtg atgcgctcgc cgatcgagcg gtcgaccagc tcgagcatgc
      841 cctcgaccag cccggccggc gccaccgcca ccggcagcag cggctcggcg cgggcgaagg
      901 cgagcagccg gcgggtcagg gccgcggcgc gggtggcgcc ttccagcgca ttgtcgagat
      961 ggaactcgac ctcgcggcgg gggccggtca gcttgcgccg ggcgaggtcg aggccgccga
     1021 cgacgacggc gagcatgttg ttgaagtcgt gggcgatgcc gccggtcagc tggccgaccg
     1081 cctccatctt ctggacctgg cgcaactgcg cctcggcagc ctcgcgctcg gcggactgcg
     1141 ccttgagctg ctcataggcc tggctcagtt cgctggtgcg ctcgagcacc gcttcctcca
     1201 gggcgcgggc ccggttcgcc tcgctgtcgg cctcgcgcag ggcctcgagc cgctcgctga
     1261 tcgcccggaa ggcgagccag ccgagcacga tcgcgccgac tccgatcgcc accgccagcc
     1321 agcccagcca tttggtcagc tggtcggagc gggttccgga caggcgggtg gcgttcatcc
     1381 ggttgcgcag gtcgtcgcgc tcggcggtcg cgatctgggc gagcaggttg cgcatctgct
     1441 ggccggtcgg agcccgggcg gcgccgtaga agagcgagat gccgccgctt ccgcgcttgg
     1501 cctgagcggc gagggcggcc ggggcgagct cgcggccgcg ggcctcgaac agccgctcga
     1561 gcgcctcgac ccgggcgagc tgctcggggt tgctgcgcac ctgccgccgc aactcgccga
     1621 tcagctgccg cgcctggcgc cattcggtgc cgtagacgct gccgctggtg gcgacgtcct
     1681 cgtcgagcac gtagcggccg agcgcggcct cggcgcgggc gatggtgccg tcgacggtcc
     1741 gggtcaggag catgacgtcg tagctgtggc gctcggaagc gagcgcctcg tcgcgcaccc
     1801 ggttggacga agtcaccatc agcatcagga cgacgagaag cagcgccgcg agcaccgcgc
     1861 ccgcgctcgc caacaccccg cgccacgccc cggaagatcg ttcctcggac atggcgtaca
     1921 tgtcctcacc cgccatggcc gcgagtctag catgcgggga gccgatggaa agaaggttcc
     1981 acgcccccgc cttcgattcg ccgcgctttg gggggcgatg gtcgtgccag ggaggccggg
     2041 actatccgaa acgccggcgc ctctgcctcc cctccctgga agggaggggc tgggggtggg
     2101 tgccgccgag gcactcggcg gctcgctgca ggatcgcctc ggcgactccg tcgggattgg
     2161 tgccgacgtc gctgttccag aagcggatga cggtccatcc cgccgcctcg agaaagcgcg
     2221 tgcgcacgtc gtcgcggcgc gagccgatat gctggctgcc gtctatctcg acgaccagct
     2281 tcgcgcgtcg gcaggcgagg tcggcaatat atgggccgat gggagctggc gggtgaattt
     2341 cgggcggtag cgcgagagga gccgccacag aatatgctcc tccctcgttg cgctggag
//
LOCUS       DASVQZ010000320         5587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_835720, whole genome shotgun sequence.
ACCESSION   DASVQZ010000320 DASVQZ010000000
VERSION     DASVQZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835720"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1338
                     /gene="aroA"
                     /locus_tag="VF547_08885"
     CDS             76..1338
                     /gene="aroA"
                     /locus_tag="VF547_08885"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX8262975.1"
                     /translation="MNGTVRVPGDKSISHRALICAAMARGRSRILGLSEGEDVRATAR
                     ALSAMGVRVERDGAGWSVDGVGTGGLLQPEGALDLGNSGTSARLLAGLAAAHGVAATF
                     IGDASLSRRPMERVAAPLRRIGAEVATAPGGRLPMTVRGLCPAVPIAHRLEVPSAQVK
                     TALLFAGLNIPGVTRIVEPVPTRDHGERLLKLFGADIRFGDGEIALRGEAGLKPQDLA
                     IPGDPSAAAFLAVAALVVPGSELRIEGVGINPLRTGLYDMLLGMGADLAFENRRELSG
                     EPVADLRVRHSSLKAVDVPPEIVPRMIDEFPIFFVAAAFADGITRASGLGELRFKESD
                     RIAAMVSGLGRIAPEAAASGDSVFVRGTAGGPLAGGVAIDPRLDHRVAMSFAVAGLHS
                     RQPVSVAGMSAADSSFPGFAALLEALAR"
     gene            1335..1970
                     /gene="cmk"
                     /locus_tag="VF547_08890"
     CDS             1335..1970
                     /gene="cmk"
                     /locus_tag="VF547_08890"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX8262976.1"
                     /translation="MIVAVDGPAASGKGTIAKALARHYGLPHMDTGLLYRAVALNLLR
                     WDGDPESEFSALRACDFAQMDFTDPELKSEAVGGIASRISAYPLVRQALVERQRAFAN
                     QEGGAVLDGRDIGSVIAPDADAKLFVTASPEVRARRRYDELLRMGLHAEYEEVLLDIR
                     ARDERDRNRAAAPLLQAQDAVLLDTSGMSAEAAVAAAIAAVEARLGRAEAS"
     gene            complement(2070..3383)
                     /locus_tag="VF547_08895"
     CDS             complement(2070..3383)
                     /locus_tag="VF547_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8262977.1"
                     /translation="MSRLLLPLAALLAASCSDAGGKDGRAEIYLQRFFGECGAQYGSA
                     TDVSKVEGECGIITAMINKFNATNPDVHVSSNVVAWPGYPQLSAQMAARDPPDLVTMH
                     TGMISDYAAEGLLEPIEPYLAEAGIAPSALTEASRGAVTIGARLYGLPFDTHGGLFHI
                     NTKLFAQAGLMRGGKPVLPTSPEEMMAQARQFTARTGKPYLIQSLVGDNAYAARNVYT
                     YLLAQDAVLFPTRKRIRLQTPEAERIVDFFRAITASGVSTRNQDTPAAIASFMSGEGG
                     IYPTGTWMIGQFEAESNTPGRPLYKSYTVYPYPRLWGAEVSYVSGHAWVVPRRERTPE
                     QRRAIGRFFRFIAEHNFDWARTGHLPAFKAVVESPAFKALPHRTNIAPVASTGRPLPG
                     GVQRQNAIEGLVGEEVAAAFNGQKSTKRALADAERRVNELLAGLD"
     gene            complement(3380..4216)
                     /locus_tag="VF547_08900"
     CDS             complement(3380..4216)
                     /locus_tag="VF547_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8262978.1"
                     /translation="MAAEARSARWSPLTLVAILLAAVVMLAPLIWTLLLSLKANEALI
                     GNSGAALSPPYTLENYRAILQGNQVFRWLINSLIVSGGTTIGVLVLCSLAGYGFARLD
                     FPFRRTLFIFVLLGLAIPEQAVILPQHQLFAELRLHNSYPGLILPGLAGPFGVFFMTQ
                     YMRAIPRDLDEAALLDGASRLRLFWKVLLPLTLPAQATLGVFTFLASWNAYWWPLISA
                     TRSDMYTLTVGLAAAQMNYAQTSGLGFLMAQAVFASIPIFIVYILFQKQIVRAMAGTA
                     VR"
     gene            complement(4206..5057)
                     /locus_tag="VF547_08905"
     CDS             complement(4206..5057)
                     /locus_tag="VF547_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8262979.1"
                     /translation="MLFVLPFLTAYVVILIYPLLRGMALSVQRVDLFGGGTFVGLDNY
                     ARLFADGTFIRSILNTFALALMIVPLLTALALALALALNRATRGAAVFRGVFFSSSVL
                     SVTIVTLIWRFVLAPDAGLLGETAQAIGAEPIPFLSHPTWSLWALAITTIWWSIGLPM
                     MLFLAGLQQIPGDVYEAAALDRAGRWTTLWRVTLPALKRTVVLVVMLQTAAQLQIFGQ
                     AQLLTGGGPGGATRTMVLFMFESAFARWELGYATAAAEILFLLIVLITLTQYVTTMRG
                     SETERGR"
     gene            complement(5090..>5587)
                     /locus_tag="VF547_08910"
     CDS             complement(5090..>5587)
                     /locus_tag="VF547_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX8262980.1"
                     /translation="DRIVVMNDRRIQQVGPPMEIYSRPANTFVARFVGSPAMTIARAT
                     LVDGGGSGARVRLGHGSEIETRVPRAALPTGAELEIGLRPESVRVAGGGASATAAKVE
                     LVERLGERTLIYARLADGQAISAEDEGLSRVRIGDTVGLRIDGASAHIFGPAGAGYHA
                     EARPD"
BASE COUNT          866 a         1935 c         1941 g          845 t
ORIGIN      
        1 acgggccgct gtcaaaagcc aagcccgatg gagacaagcg gggcccggcc gatcgagttc
       61 tcagcctccg gccccttgaa cggcaccgtc cgcgtcccgg gcgacaagtc gatctcccac
      121 cgtgccctga tctgcgccgc gatggcccgc ggccgaagcc gaatcctcgg cctgtcggaa
      181 ggtgaggacg tccgcgccac cgcccgcgcg ctttcggcga tgggggtgcg cgtcgagcga
      241 gacggcgccg gctggtcggt cgacggagtc ggcaccggcg gcctcctcca acccgaaggc
      301 gcgctcgacc tcggcaacag cggcacttcg gcccggctcc tggcggggct cgccgccgcc
      361 cacggcgtcg ccgcgacctt catcggcgac gcctcgctgt cgcggcggcc gatggagcgg
      421 gtggctgcgc ctctcaggcg gatcggcgcc gaggttgcga cggccccggg gggccgcctg
      481 ccgatgaccg ttcggggcct gtgcccggcg gttccgatcg cccaccgcct cgaagtgccc
      541 tcggcgcagg tcaagaccgc cctcctcttc gcgggcctca acatcccggg agtcacccgc
      601 atcgtcgagc cggtcccgac ccgcgaccat ggcgagcggt tgctgaagct tttcggcgcc
      661 gacatccgct tcggcgatgg tgagatcgcg ctgcgcggcg aggccggcct gaagccccag
      721 gacctcgcca tccccggaga cccctcggcc gccgcgttcc tggcggtcgc cgcgctcgtc
      781 gttcccggct ctgagcttcg gatcgagggc gtcgggatca atccgctccg gaccggtctc
      841 tacgacatgc tgctcgggat gggcgccgac ctcgccttcg agaatcgccg ggaattgagc
      901 ggcgaaccgg tcgcggacct tcgagtccgc cattcgtccc tgaaggccgt ggacgtcccg
      961 cccgagatcg tcccgcgcat gatcgacgaa ttcccgatct tcttcgtcgc cgctgccttc
     1021 gccgacggaa tcacccgagc ctccggcctc ggcgagctcc ggttcaagga atcggaccgt
     1081 atcgccgcca tggtctccgg gctcggccgg atcgcgcccg aggcggcggc gagtggggac
     1141 tcggtcttcg tccgggggac tgccggcggg ccgctcgccg gcggggtggc gatcgacccg
     1201 cgcctcgatc accgagtcgc gatgagcttc gcggtggctg gcctccattc gcggcagccg
     1261 gtatcggtcg ccggcatgtc cgccgccgac tccagcttcc ccggcttcgc cgccctgctc
     1321 gaggcgctcg cccgatgatc gtcgccgtcg acggtcccgc cgccagcggc aagggcacga
     1381 tcgccaaggc gctcgcccgc cattacggcc ttccgcacat ggacaccggc ctgctctacc
     1441 gggcggtggc gctcaacctg ctgcgctggg acggcgatcc ggagagcgaa ttctccgccc
     1501 ttcgcgcctg cgacttcgcc cagatggact tcaccgatcc cgagctgaag agcgaggccg
     1561 tcggcggcat cgcctcgcgg atctcggcct atccgctcgt ccgccaggcc ctggtcgagc
     1621 gccagcgcgc cttcgccaat caggagggcg gcgccgtcct cgacggccgc gacatcggct
     1681 cggtcattgc gcccgacgca gacgccaagc tgttcgtcac ggccagcccg gaggttcgcg
     1741 ccaggcgccg ctacgacgag ctcctgcgaa tgggcctcca cgcggaatat gaagaggtgc
     1801 tgctggacat ccgggccagg gacgaacggg accggaaccg tgccgccgcg ccgctgctgc
     1861 aggcacaaga cgcggtgctt ctggatacaa gcggaatgag cgccgaagcg gcggtggcgg
     1921 ccgcgatcgc cgcagtggag gcccgtctgg gccgggcgga ggcttcctag agccgatccc
     1981 accctagccg gcctctccat gccggcccgg atacaaattc ctttccaagg gcccggaatg
     2041 ttggccgcag caagtccaag cgtggcggtt cagtccaatc ccgccagcag ctcgttgacc
     2101 cggcgctcgg cgtcggcgag ggcgcgcttc gtcgacttct gcccgttgaa cgccgcggcg
     2161 acctcctccc cgaccagccc ctcgatggcg ttctggcgct ggacgcctcc gggcagcggc
     2221 cggcctgtgc ttgcgaccgg ggcgatgttg gtgcggtggg gaagcgcctt gaaggccgga
     2281 ctctcgacca cggccttgaa ggccggaaga tggccggttc gcgcccagtc gaaattatgc
     2341 tcggcgatga agcggaagaa acggccgatg gctcggcgct gctcgggagt ccgttcgcgc
     2401 ctgggcacga cccaggcatg gccgctcaca tagctcacct cggcgcccca cagccgggga
     2461 taagggtaga ccgtgtagct tttgtagagc ggacgtccgg gcgtgttcga ctccgcttcg
     2521 aactggccga tcatccaggt cccggtcgga tagatgccgc cttcgccgga catgaagctg
     2581 gcgatggcgg ctggcgtatc ctggttgcgc gtgctgacgc ccgaggcggt gatcgcgcgg
     2641 aagaagtcga cgatccgctc cgcctccggc gtttgcaggc ggatcctttt gcgggtcggg
     2701 aacagcacgg cgtcctgcgc caggaggtag gtgtagacgt tgcgggcggc ataggcgttg
     2761 tcgccgacca ggctctggat cagatagggc ttgccggtcc ttgcggtgaa ctggcgtgcc
     2821 tgcgccatca tctcttccgg cgaggtcggg aggaccggct tgccgcccct catcagtccg
     2881 gcctgcgcga acagcttcgt gttgatgtgg aagaggccgc cgtgcgtgtc gaacggcagg
     2941 ccgtacagcc gcgcaccgat cgtcaccgcg ccccggctcg cttcggtcag cgcggagggc
     3001 gcgattcccg cctccgccag atagggctcg atcggctcca ggaggccttc ggcggcatag
     3061 tccgagatca tgcccgtatg catggtgacg agatcgggcg ggtccctcgc cgccatctgg
     3121 gcggacagct gggggtagcc gggccaggcg acgacgttgc tgctgacgtg gacgtcgggg
     3181 ttggtggcgt tgaacttgtt gatcatcgcg gtgatgatcc cgcattcgcc ttccaccttc
     3241 gacacgtcgg tcgccgagcc atattgggcg ccgcactcgc cgaagaagcg ctgcagatag
     3301 atttcggcgc ggccgtcctt gccgccggcg tcgctgcagc tcgcggcgag gagcgcggcc
     3361 aatggcagga ggaggcggct cacctcaccg ccgtcccggc catggcgcgg acgatctgct
     3421 tctggaacag gatgtagacg atgaagatcg ggatcgaggc gaacaccgcc tgagccatca
     3481 ggaagccgag gcccgacgtc tgcgcatagt tcatctgcgc cgcggcgagg ccgacggtga
     3541 gcgtgtacat gtcgctgcgc gtcgccgaga tgagcggcca ccaataggcg ttccacgagg
     3601 cgaggaaggt gaacacgccc agggtcgctt gcgccgggag ggtgagcggc agcagcacct
     3661 tccaaaagag cctaagccgc gacgccccgt cgagcagcgc cgcctcgtcg aggtcccgcg
     3721 ggatggcgcg catatattgg gtcatgaaaa agaccccgaa cggcccggcg agcccgggca
     3781 ggatcagccc cggatagctg ttgtgaagcc tcagctcggc gaacagctgg tgctgcggga
     3841 ggatcaccgc ctgctcgggg atggcgagtc cgaggaggac gaagatgaag agcgtccggc
     3901 ggaacgggaa gtcgagccgg gcgaatccgt agccggcgag gctgcacagg acgaggaccc
     3961 cgatcgtggt cccccccgag acgatgaggc tgttgatcag ccagcggaac acctggttcc
     4021 cctgcaggat cgctcggtag ttctcgagcg tgtagggcgg cgacagggcc gcgccgctgt
     4081 tgccgatcag cgcctcgttc gccttgagcg acagcaggag cgtccagatg agcggcgcga
     4141 gcatcacgac ggcggccagg aggatcgcga ccagggtcag cggcgaccag cgggcgctcc
     4201 tcgcctcagc ggccacgctc ggtctccgat ccgcgcatgg tcgtcacata ttgggtgagg
     4261 gtgatcagga cgatgagcag gaacaggatt tcggccgcgg ccgtcgcata gcccagctcc
     4321 catcgggcga aggcggattc gaacatgaac agcaccatcg tccgcgtcgc gccgcccggg
     4381 ccgccgccgg tgagcagctg tgcctggccg aaaatctgaa gctgcgccgc cgtctggagc
     4441 atgacgacga ggacgacggt gcgcttcagc gccggcaagg tcacccgcca aagggtggtc
     4501 cagcggcccg cccggtcgag cgctgcggct tcgtacacgt cgcccgggat ctgctggagg
     4561 cctgccagga acagcatcat cggaaggccg atcgaccacc agatggtcgt gatcgccagc
     4621 gcccagagcg accaggtcgg gtggcttaga aacgggatcg gctcggcgcc gatcgcctgc
     4681 gccgtctcgc ccaggagacc ggcgtccggc gcaagcacga agcgccagat cagggtgacg
     4741 atggtgacgg acagcaccga ggaggagaag aacacgccgc ggaataccgc ggctccgcgg
     4801 gtcgcccggt tgagcgccag cgccagcgcc agcgccagcg ccgtgagcag gggaacgatc
     4861 atcaaggcga gtgcgaaggt gttgaggatc gagcggatga aggtgccgtc ggcgaacagc
     4921 cgcgcataat tgtcgagacc gacgaacgtc ccgccgccga acaggtcgac tctctggacg
     4981 ctgagggcca tgcccctgag cagggggtag atcaggatga cgacataggc ggtcaggaac
     5041 ggcaggacga agagcatccc gctccagtcc ctgcgcgccc cgcccccctt cagtcgggcc
     5101 gcgcctcggc gtggtagccg gcgccggcgg ggccgaagat atgcgccgac gcgccgtcga
     5161 tcctgagacc gaccgtgtct ccgatcctga cccggctcag cccctcgtcc tcggcgctga
     5221 tcgcctggcc gtccgccagc cgggcgtaga tgagagtccg ctccccgagc cgctcgacca
     5281 gttccacctt ggccgccgtg gcgctggcgc cgccccccgc cacccgcacg ctctccggcc
     5341 tgagcccgat ctccagctcg gcgcccgtcg gcagcgccgc ccgcggcacc cgtgtttcga
     5401 tctccgagcc gtggccgagc ctcaccctcg caccggaccc gccgccatcg actaaagtcg
     5461 cccgcgcgat cgtcatcgca ggcgagccga cgaagcgcgc gacgaaggta ttggcggggc
     5521 ggctgtatat ctccatcggc gggccgacct gctgaatcct gcggtcgttc atcaccacga
     5581 tgcggtc
//
LOCUS       DASVQZ010000321         4112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_932462, whole genome shotgun sequence.
ACCESSION   DASVQZ010000321 DASVQZ010000000
VERSION     DASVQZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932462"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VF547_08915"
     CDS             complement(<1..1601)
                     /locus_tag="VF547_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HEX8262981.1"
                     /translation="MPVYIRTRDEFAINSFWADNQTAPSVATFADGGFVVIWGTLDGA
                     QDGDSSAIKGQLFDSAGSKVGTEFRVNTLPAGSQFTASVATLGNGNFVVTWVTSGTDP
                     YAMDGSDGIKAQMYNSWGWPVGSEFQVDSAFTGSMFTPVVAALANGGFVISWADSNGF
                     NIKAQIFDSAGSPVGDNFTLNANTSGSQDSADITALANGGFVATWTTQDSSADGSGEA
                     VKAQVFSATGARVGGEFLVNSQAAEGQYDASVTALAGGGFVITWATRDPAQDGSSGAV
                     KGQIFNSSGAKVGGEFLVNTAAADIQREAVVTGTPDGGFVVAWTNYSSSQDGSGFAIK
                     AQAFDSAGAKVGSEMLVNTLTSGSQFLPDIATLADGRVIVTWTSTTGDGEGYAVRAQI
                     LGTQLAEPVPNARPIIISPGGTSASLMVEEGTAFVTTVVASDDGEPTDLRYSITGGPE
                     AHLFTINAVTGALSFIQAPDHVEGESNFYTVVVSASDGELDNWQMIHVGVRHVNHVTI
                     VSDGGGQYARVSIDENQTGVTLVGAV"
     gene            complement(1828..2394)
                     /locus_tag="VF547_08920"
     CDS             complement(1828..2394)
                     /locus_tag="VF547_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262982.1"
                     /translation="MAATADIEGGRPRNRWRPALWGGAALLLLAPAVATRFTDEMNWG
                     PGDFLHLAALLAAACAIFELLARTTDRRAYRAGAGVALATGVLLIWINLAVGIIGTED
                     NRANLMFAGVLLVAVLGAALARGRAEGMARALAATAAAQAAAGAAALAAGWGASDPSW
                     PFEIIGLTGLFAAAWLLAAWLFRRSLKA"
     gene            2601..3143
                     /locus_tag="VF547_08925"
     CDS             2601..3143
                     /locus_tag="VF547_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262983.1"
                     /translation="MSQNIVSAVFDSHSEAEAAVRELRGAGVSDSALSVIARRQEGGG
                     GDYGGADTHEVKDKGEGLLKGAAAGAGVGALLGIAALAIPGVGPLAAAGAIASSAIPE
                     AAAIGAGAGALTGGLTGLLTKHGVSDDDASYYEGRIKEGGVFVSVDTGAAGLDRETAQ
                     DILYRAGGHSAARSKMGATL"
     gene            3325..3807
                     /locus_tag="VF547_08930"
     CDS             3325..3807
                     /locus_tag="VF547_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX8262984.1"
                     /translation="MRRYRPSRPWPVGGLALAAAAAAVLATGGLSSVLDGGPDPAFAA
                     RIARSVASGPSPIEARAAPPPAAPAASVPAASEPAPPAAAWQIQVGAFRNVVAAEAHL
                     RSLESGVPELARLTATHRLRGGLNRVRIGGIADETAARGLCARIVAAGNGCFVVAPES
                     "
BASE COUNT          616 a         1417 c         1473 g          606 t
ORIGIN      
        1 acggcgccga ccagggtcac gccggtctgg ttctcgtcga tcgacaccct cgcgtactgg
       61 ccgccgccgt ccgagacgat ggtgacgtgg ttgacgtggc ggacgccgac atggatcatc
      121 tgccagttgt cgagctcccc gtcgctcgcc gagacgacga cggtgtagaa attgctctcg
      181 ccttcgacat gatcgggcgc ctggatgaag gagagcgcgc cggtcaccgc gttgatggtg
      241 aacaggtgcg cctcggggcc gccggtgatc gaatagcgaa gatcggtcgg ctcgccgtcg
      301 tcggaggcga cgaccgtggt cacgaaggcc gtgccttcct cgaccatgag cgaggcggag
      361 gtgccgcccg gggagatgat gatcgggcgc gcgttcggca ccggttcggc gagctgcgtc
      421 cccaggatct gggcgcgcac ggcatagccc tcgccgtcgc cggtggtgct ggtccaggtg
      481 acgatgaccc ggccgtctgc cagggtggcg atgtcgggca ggaattgcga gccgctggtc
      541 agcgtgttga ccagcatctc cgaaccgacc ttggcgccgg cggaatcgaa ggcctgggcc
      601 ttgatcgcga agccgctgcc gtcctggctg ctgctgtagt tggtccaggc gacgacgaac
      661 ccgccgtcgg gcgtgccggt gaccaccgct tcgcgctgga tgtcggcggc ggcggtgttg
      721 acgaggaact cgccgcccac cttcgcgccc gaggagttga agatctgccc cttcaccgcg
      781 ccgctgctgc cgtcctgcgc cgggtcgcgg gtcgcccagg tgatgacgaa gccgccgccg
      841 gcgagcgcgg tgacgctcgc atcatattgc ccttcggcgg cctggctgtt gaccaggaac
      901 tcgccgccga cccgagcgcc ggtggcgctg aagacctggg ccttgaccgc ctcgccgctg
      961 ccgtcggccg agctgtcctg ggtcgtccag gtcgccacga agccgccgtt ggcgagggcc
     1021 gtgatgtcgg ccgaatcctg gctcccgctg gtgttggcgt tgagcgtgaa attgtccccc
     1081 accggcgagc cggcggagtc gaagatctgc gccttgatgt tgaagccgtt cgaatcggcc
     1141 cagctgatca cgaagccgcc gttggcgagc gccgcgacga cgggcgtgaa catcgagccg
     1201 gtgaaggcgg aatcgacctg gaactcgctc ccgaccggcc agccccacga attgtacatt
     1261 tgggccttga ttccgtcgct gccgtccatg gcgtagggat cggtgccgct ggtcacccag
     1321 gtgacgacga aattgccgtt cccgagggtc gcgaccgaag cggtgaattg cgaccccgcc
     1381 ggaagcgtgt tgacccggaa ctcggtcccg accttgcttc ccgccgagtc gaagagctgc
     1441 cccttgatcg cgctgctgtc gccgtcctgg gcgccgtcga gggtgcccca gatgacgacg
     1501 aagccgccgt cggcgaaagt ggcgacggac ggcgccgtct gattgtcggc ccaaaagctg
     1561 ttgatcgcga actcgtcgcg cgtacggata taaaccggca ttgatttccc cccacccaat
     1621 tgccggcgac gttaggaaac gccgccttta caatgtgtaa agctctgaaa attttatcat
     1681 taagcgccgg cacttccccc ctgcgagtcg cgaccgaccg aaggccttat agcacaaaaa
     1741 gagaacaaac ggaagcagta tgttccactt ccgttcgttt gctggctggc gggggcggcc
     1801 gcccgagctt gagcggcgag gggcggctca ggccttgagg gaccggcgga acagccatgc
     1861 cgccagcagc caggcggcgg cgaagagtcc ggtgaggccg atgatctcga acggccagct
     1921 cgggtcggag gcgccccatc cggcggcgag cgcggccgct ccggccgccg cctgggcggc
     1981 cgccgtcgcc gccagggcgc gggccatgcc ctcggcccgg ccgcgcgcga gcgcggcgcc
     2041 gaggacggcg acgaggagca cgccggcgaa catcaggttc gcccgattgt cctcggtgcc
     2101 gatgatcccg acggcgaggt tgatccagat gagcaggacc cccgtcgcca gcgcgactcc
     2161 ggcccccgcg cggtaggccc ggcggtcggt cgtccgcgcc agcaactcga agattgcgca
     2221 ggccgcggcc agcaaggcgg cgagatggag gaagtcgccc gggccccagt tcatctcgtc
     2281 ggtgaagcgg gtcgcgaccg ccggcgccag caggagcagc gcggcgccgc cccacaaggc
     2341 cggccgccag cggttgcggg gccgtccgcc ctcgatgtcc gcggttgctg ccatggcctt
     2401 gccttccttc gcgctccgaa tccgagcctg gcctatgcgc aaggcctttg cagccccgat
     2461 ggcccgagca aaggagaaaa tgtgcagaag ggcccgcgcc ttgagacgaa cggccgaaaa
     2521 ggggccgagc cgaggaacgg gcgccttgcg ggagcggttg ggtggacgag cggggccccc
     2581 gaaagagtag gaacagcgta atgagccaga acatcgtgtc cgccgtcttc gacagccatt
     2641 ccgaagcgga ggccgccgtt cgcgagcttc gcggcgcggg cgtcagcgac agcgccctct
     2701 ccgtgatcgc ccggcgccag gaaggcggcg gcggcgatta tggcggcgcc gacacccatg
     2761 aggtcaagga caagggcgaa ggcctgctca agggcgcggc ggccggcgcc ggagtcggcg
     2821 ccctgctcgg catcgccgcg ctcgccatcc ccggcgtcgg cccgctcgcc gcagccggag
     2881 ccatcgcctc gtcggccatc cccgaagccg ccgccatcgg cgcgggcgcc ggagccctga
     2941 ccggcggcct caccggcctc ctcaccaagc acggcgtcag cgacgacgac gcgagctatt
     3001 acgaaggccg gatcaaggaa ggcggggtct tcgtctcggt cgacaccggc gcggccggcc
     3061 tcgaccgcga gaccgcccag gacatcctct accgcgccgg cggccacagc gcggcccgtt
     3121 cgaagatggg cgcgacgctc tgatcgtcac gcacgaaagg gagcggcccc gccccagcgg
     3181 cgggggcgct tctctttgtc cgggactcgc ttcgggaacc cgggcgaggg cccggcgttc
     3241 cggcaaccca ctgagaatcg gacaagaacc attcgccacc agaagatcct gatcgctctg
     3301 gagcgcgaag ccgggacggc ggcggtgcga cgctatcggc cgtcgcggcc ctggccggtg
     3361 ggcggcctgg cgctggccgc agcggccgcg gcggtgcttg cgacgggcgg gctttcatcg
     3421 gtcctggacg gcggacccga tccggccttc gcggcccgga tcgcgcgaag cgtggcgagc
     3481 ggtccctcgc cgatcgaggc gcgcgccgcc cctcccccgg ccgcccctgc ggcttccgtc
     3541 cccgccgctt ccgagcccgc tccgcccgcc gccgcctggc agatccaggt cggcgccttc
     3601 cgcaacgtcg tggcggcgga ggcgcatctg cggtccctgg agagcggggt gccggagctt
     3661 gcgcggctga cggcgaccca ccggcttcga ggcggcctca atcgggtccg gatcggcggc
     3721 atcgcggacg agacggcggc acgcgggctg tgcgcgcgca tcgtggcggc cgggaacggc
     3781 tgcttcgtgg tggcgcccga aagctagggc agcgccaccg cgccgagggc gatcgcggcc
     3841 tggccggcga acagggccgc ggcgatcagc aggtcgatcg cgcgcgccat catctcgtcc
     3901 tgcatcggcg gctctcctcg ccacccggct gtgcggccgc cgcgattgag aaaggctcat
     3961 cggaaatcgc taccgcgccg ttcaccatct ttatccggat cgaagcgggc ggaccccgcg
     4021 ttcgtcacca ggcgctgcct ccactgaggc cggtgggcgc gggtatgcgt ggtggagtgg
     4081 atttggccgg cttggaacag ggcattgctg gc
//
LOCUS       DASVQZ010000322         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_66557, whole genome shotgun sequence.
ACCESSION   DASVQZ010000322 DASVQZ010000000
VERSION     DASVQZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66557"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..176)
                     /locus_tag="VF547_08935"
     tRNA            complement(100..176)
                     /locus_tag="VF547_08935"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Asp,seq:gtc)
     gene            complement(224..1432)
                     /locus_tag="VF547_08940"
     CDS             complement(224..1432)
                     /locus_tag="VF547_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8262985.1"
                     /translation="MKRVQGEEGGAGAAPSDHGEIGLETEAIAFRRDRLLAALTLIAG
                     AGLTVALPFALRAGAEFFLPVTAALVIAIALVPMLEWLERRRVPSTLAAFACVLLLIA
                     MANVAVASIVLPAAEWVRLLPERIGRVRDTLAPLMEVYSELERFINDIVEQFGTRATN
                     QRTVALETPNSLLDIIATSAPFAAIQMFFAVLVIFFFLAGWTRMRRRTITARSSFDGA
                     MTTARAIQQVVDATSTYLGTITVVNIGMGLAVALILYMLDMPTPLMWGGIVAVLNYIP
                     YLGPIASALLLALGGLMAFADPWYAFLPALCFIGVHLVEANLITPFFVGRRLTINPLL
                     ILVALSFWAWVWGTTGALLAVPLLIIMKTVLDAAGKPDIAGFLFEEGTLTGPHHEEHG
                     DNERIAATKA"
     gene            1604..>2223
                     /locus_tag="VF547_08945"
     CDS             1604..>2223
                     /locus_tag="VF547_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262986.1"
                     /translation="MTAFTAACAGVFAGPAFAAGTAAGTNITNVATATYDLPNGGEAS
                     VDSNVVTLKVDELIDVTVSWGDPADVATSPGLTGQPLKFTVTNGGNGAESFTLAALAN
                     GGGDDFDPAITGIVLDSNGNGAYDAGVDTVYSSGSNDPQLEPDQAITVFVLSSIPAGA
                     GDGRALDEGDAGPGDAIIAVVLGVAAGGSHDGVAAALAEAGEAGSGR"
BASE COUNT          388 a          755 c          762 g          318 t
ORIGIN      
        1 cgggcttcct cagctgcgca taatccagcc cgatgcggtc gtagcggctc gccatcgggc
       61 gcttatggcg gatggcggcc gggtcaagaa taaggaaaat ggcgcgagtg acggggctcg
      121 aacccgcgac ctccggcgtg acaggccggc gctctaacca actgagctac acccgcgtgg
      181 gagccggcga actatgccct gccccccgcc ctgtcaagcg aggctaggcc ttggttgcgg
      241 cgatccgttc attgtcgcca tgctcctcgt gatggggtcc ggtgagcgtc ccctcctcga
      301 acaggaagcc ggcgatgtcg ggcttgccgg cggcgtcgag caccgtcttc atgatgatga
      361 ggagcggcac cgcgagcagc gccccggtcg tcccccagac ccaggcccag aagctcagcg
      421 cgacgaggat cagcagcggg ttgatggtca ggcgccggcc gacgaagaag ggcgtgatca
      481 ggttcgcctc gaccaggtgg acgccgatga agcacagggc cggaaggaag gcgtaccagg
      541 ggtccgcgaa cgccatcagg ccgcccagcg caagcagcag ggccgaggcg atcgggccga
      601 gatagggaat gtagttgagc acggcgacga tcccgcccca catcagtggc gtcggcatgt
      661 cgagcatgta gaggatgaga gcgaccgcga gccccatgcc gatgttcacc acggtgatcg
      721 tgccgagata ggtcgaggtc gcgtcgacca cctgctggat ggcgcgggcg gtggtcatcg
      781 ccccgtcgaa gctcgaccgc gcggtgatcg tgcgccggcg catccgggtc cagccggcaa
      841 ggaagaagaa gatcaccagc accgcgaaga acatctggat cgccgcgaac ggggccgagg
      901 tggcgatgat gtcgagaagc gagttcgggg tctccagcgc cacggtgcgc tggttggtgg
      961 cgcgggtccc gaactgctcg acgatgtcgt tgatgaagcg ctcgagctcg gaatagacct
     1021 ccatcagcgg ggccagggtg tcgcggaccc gtccgatccg ctccggcagc agccggaccc
     1081 attccgccgc cggcaggacg atcgaggcga cggcgacatt ggccatggcg atcaggagca
     1141 gcacgcaggc gaaggcggcc agggtcgagg gcacgcgccg ccgctcgagc cattcgagca
     1201 tcggcacgag ggcgatggcg atgaccaggg ccgcggtcac cggaaggaag aattcggcgc
     1261 cggcgcggag cgcgaacggc agggcgacgg tgaggccggc cccggcgatc agggtcaggg
     1321 cggcgagcag gcggtcgcgg cgaaaggcga tcgcctccgt ctcgaggccg atctcgccat
     1381 ggtccgacgg cgccgcgccg gcgccgcctt cctccccttg gaccctcttc atattcgtta
     1441 ccgccgtcct tcgagcttgc cttaatcgca ctgactaatc ggccgttaac gcattcttag
     1501 ccgctctccc cgcattagcg catcgaaccg agctcgtggg ggagccacaa tgcgttatca
     1561 ggctacgggc gccaaatcat cggcgttccg caaaaatgca gccttgaccg ccttcacggc
     1621 ggcctgcgcg ggcgtgtttg cgggccccgc cttcgcggcg gggacggctg cggggaccaa
     1681 catcaccaat gttgccaccg ccacctacga ccttcccaac ggcggcgagg ccagcgtcga
     1741 ttcgaacgtc gtcaccctca aggtcgacga gctgatcgac gtcaccgtct cctggggcga
     1801 cccggccgac gtcgccacct cgccgggcct gaccggccag ccgctcaagt tcaccgtcac
     1861 caacggcggc aacggcgccg agagcttcac cctcgccgct ctcgccaacg gcggcggcga
     1921 cgatttcgac ccggcgatca ccggcatcgt cctcgatagc aacggcaacg gcgcctacga
     1981 cgccggggtc gacaccgtct attcgagcgg cagcaacgac ccccagctcg aacccgatca
     2041 ggcgatcacc gtcttcgtcc tgtcgagcat cccggccggc gccggcgacg gccgcgctct
     2101 tgacgaaggc gacgctggcc ctggcgacgc gataatagcc gtcgtcctcg gagtcgccgc
     2161 cggtggctcc cacgacggcg tcgccgccgc cctggccgag gccggcgaag ctggttccgg
     2221 gcg
//
LOCUS       DASVQZ010000323         5846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_66836, whole genome shotgun sequence.
ACCESSION   DASVQZ010000323 DASVQZ010000000
VERSION     DASVQZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66836"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF547_08950"
     CDS             <1..322
                     /locus_tag="VF547_08950"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052357.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX8262987.1"
                     /translation="VMLEEVIPATRRALQRSGMSIDDIDLYEVNEAFAPVPLAWLREL
                     GADPAKLNVNGGAIALGHPLGASGTKLMTTLVHALKARGKRFGLQTMCEGGGLANVTI
                     VEAL"
     gene            complement(339..818)
                     /locus_tag="VF547_08955"
     CDS             complement(339..818)
                     /locus_tag="VF547_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262988.1"
                     /translation="MRILLLLAAGSAAVGTAAPARPAADFAGRGGGAPGVAASAGGPR
                     VRSGWQVYVRPGLGRPGHHRGRFGRPGRDGIDFDGGYGLAGPGGIVAPWGDGFFAPGG
                     GEVRMKAGRPVYDYDRSYPYEWPSAAGGRGRRWEVGFVSEPPPHCTIEHGVRVCRGW"
     gene            1037..1456
                     /gene="rplQ"
                     /locus_tag="VF547_08960"
     CDS             1037..1456
                     /gene="rplQ"
                     /locus_tag="VF547_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX8262989.1"
                     /translation="MRHRNAGRKLQRTSSHRAALFRNMAAALIKHEQITTTTAKAKEL
                     RPYVEKLITLAKKGGLSNRRLAHSRLLDDTQLQKLFNVLAPRYAGRDGGYTRVIKAGI
                     RASDAAPIAIIELVDRDVSAKGQDSGPVEREEEYEAA"
     gene            1593..1769
                     /locus_tag="VF547_08965"
     CDS             1593..1769
                     /locus_tag="VF547_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8262990.1"
                     /translation="MAERKRELIDTGNNKLFVRRDKEGQFKEVVDVGASLAADRRQHA
                     KTKVKSGQGDRGDR"
     gene            1983..2738
                     /locus_tag="VF547_08970"
     CDS             1983..2738
                     /locus_tag="VF547_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX8262991.1"
                     /translation="MEIGILRTGAPPGDLEARFGSYEAMFARLLGPDFNVRAYDVVAG
                     ALPDRPDAHPAFLITGSPAGVYEPLPWIAPLLGFLREAKGRSKLVGICFGHQAMAEAY
                     GGRVEKSERGWAAGLQPYELVARRPWMGDRPPARIAVPVSHQDRVVIAPPDAEVLAHS
                     PFSPFGMLAWGSDALSMQFHPEFEPDYAKALIEYRRARLPAPDAAIASLDAPDDRAVV
                     AGWIRAFLKGNSSPAFAGEGDHPKGGGGASPDA"
     gene            complement(2802..3734)
                     /locus_tag="VF547_08975"
     CDS             complement(2802..3734)
                     /locus_tag="VF547_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8262992.1"
                     /translation="MRLGGYRTSDNVSDQGRGFGGGGFGGGGGGGGCLFALLPFVLSR
                     FGIGGVLILLLGYCALSYMGGGGGIGLTGPDPAVAPHQGGRATSNAAQACSSEPAKRF
                     TCQVLASTEDTWAKLFAANGQRYQPPTLSFYGGGGRSGCGAAQSAMGPFYCPTDRGIY
                     LDTVFFDELQSKFGAAGDFAQAYVVAHEVGHHIQNLTGVLEQASAQQRRVSRAEGNAI
                     QVRVELQADCYAGVYAANAKDAQGRPVMEPGDVEEGLRAAQAIGDDTLQRQAQGRVVP
                     EAFTHGSSAQRSQWLKRGLETGDPAACDTFGGAG"
     gene            3949..4704
                     /locus_tag="VF547_08980"
     CDS             3949..4704
                     /locus_tag="VF547_08980"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX8262993.1"
                     /translation="MFLTGKTALVTGSTSGIGLAVARALAAEGARVTLNGFGEPAEIE
                     RLKEELGAGYSGADLTRSEAIEAMMDEAGPVDILVNNAGMQHVAPIDEFPVEKWDLLI
                     ALNLTAAFHTIRLALPAMKSKGWGRVINTASAHSLVASPNKAPYVASKHGIAGLTKAV
                     ALETATTGVTVNCISPGYVWTPLVENQIPDTMRARGLTREQVMNDVLLSAQPTKKFVT
                     VDQVAAMAVFLCRDEASAITGANLSMDGGWTAA"
     gene            complement(4786..5097)
                     /locus_tag="VF547_08985"
     CDS             complement(4786..5097)
                     /locus_tag="VF547_08985"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8262994.1"
                     /translation="MRVAAMLERALAEDRRRFMRHPVHLGAGLSRANDRPGSPVTVID
                     LSTHGCGLEVEGHCEPGSRVWLKLPGLESWPARIAWAEGGRAGLSFDRPLHEAVVGRF
                     A"
     gene            5211..>5846
                     /locus_tag="VF547_08990"
     CDS             5211..>5846
                     /locus_tag="VF547_08990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8262995.1"
                     /translation="MLPAPSIIVEWETGQEGHGGRAAACLAEINRQMWEARTAFAAPP
                     ELILVFDPRQAAPGSVEAAAAEAAAGRGWPGRFELVEAPPGCDYYGKKNLGFRRSRGD
                     VALFADSDLAPEPGWLRALVEPLADPARSVVVGRTHFETDTLYERAMALFWIFDERLD
                     QHWVRPTRRLVSNNIAFRRALFAALPFPDRPTYRGQCSELGATLAGLGIVLY"
BASE COUNT          892 a         2183 c         1962 g          809 t
ORIGIN      
        1 ggtcatgctg gaagaggtga tccccgccac caggcgcgct ctccagcgct cgggcatgag
       61 catcgacgat atcgacctct acgaggtcaa cgaggcgttc gcgccggtgc cgctcgcctg
      121 gctgcgcgag ctcggagccg atccggcgaa gctcaacgtg aacggcggag caatcgcgct
      181 cggccacccg ctcggcgcct cgggcaccaa gctgatgacc accctcgtcc acgccctcaa
      241 ggcgcgcggc aagcgcttcg gcctccagac gatgtgcgag ggcggcgggc tggccaatgt
      301 gacgatcgtc gaggcgctct gacgctcccg ccctggcctc accagccgcg gcagacccta
      361 accccatgct cgatcgtgca gtgcggcggc ggctcggaaa cgaagccgac ctcccagcgc
      421 cggccccgcc cgccggcggc ggacggccat tcgtagggat aggagcggtc gtaatcgtag
      481 accggccggc cggccttcat ccgcacctcg cccccgccgg gcgcgaagaa gccgtcgccc
      541 cagggcgcga cgatcccgcc cgggccggca agaccgtagc cgccgtcgaa gtcgatgccg
      601 tcccggcccg gcctcccgaa ccggccgcgg tggtggccgg ggcggccgag gcccggccgg
      661 acatagacct gccagccgga gcggacgcga gggccgccgg ccgaagccgc aacgcccggc
      721 gctccgccgc ctcggccggc gaaatcggcg gcgggcctcg ccggcgccgc ggttccgacc
      781 gccgccgagc ccgccgccag caacaagagg attcgcatct tcattctccc ggtccgaagg
      841 cgccgcggcc cggtacgact cgcgccggct gccgagcgct tcgccttgac tttacgcccg
      901 gtttcggctt gaggaacccc cgcaggcggg gccctacagg ctccgccgtc gcattttggg
      961 atttgggcgg tctccccttt cgaccgcccg gccgcggcta cctgagacgg gccgcggacg
     1021 aacgaaggat gaagacatgc gtcaccgcaa cgccggccgc aagctgcagc gcacctcgag
     1081 ccaccgcgcc gccctgttcc gcaacatggc cgccgccctg atcaagcacg agcagatcac
     1141 caccacgacg gccaaggcga aggagcttcg cccctatgtc gagaagctga tcacgctcgc
     1201 caagaagggc ggcctgtcca atcgccgcct cgcccattcg cgccttctcg acgacaccca
     1261 gctgcagaag ctcttcaacg tcctcgcgcc gcgctacgcc ggccgcgacg gcggctacac
     1321 ccgcgtcatc aaggccggca tccgcgcctc cgacgcggcg ccgatcgcga tcatcgagct
     1381 ggtcgaccgt gacgtttccg ccaagggcca ggattcgggc ccggtcgagc gcgaggaaga
     1441 gtacgaagcc gcgtagagcc tgtcctgctc cccggcggag gccgggccgt aacccgggaa
     1501 ggccaggtcc cggcctacgc cgggaagcag cgaaggaacc agtgcgactc ccagggattc
     1561 ttcccccgat gtaataattc gggagaattt ccatggccga gcgcaagcgc gagctgatcg
     1621 ataccggcaa caacaagctg ttcgtgcgcc gcgacaagga agggcagttc aaggaggtcg
     1681 tcgacgtcgg cgccagcctc gccgccgacc gccgccagca cgccaagacc aaggtcaagt
     1741 ccggccaggg cgaccgcggc gaccgctagg gcgcagacct tcggccggcc aatgcctccc
     1801 cgcgatctcg cggggagggg gaccacccga agggtggtgg aggggttctt ttccggactc
     1861 cgaagaaccc ctccgcccgc gctgcgcgcg atccccctcc ccggcaaagg ccgggaggaa
     1921 ttgaggcgct cgccggcacg cctggtcttc cccccgcccc aatgcccgcc tagggccgtg
     1981 ccatggagat cgggatcctt cggaccggag cgccgccggg cgacctggag gcgcgcttcg
     2041 ggtcctacga ggcgatgttc gcccgattgc tcgggccgga tttcaacgtc cgcgcctatg
     2101 acgtcgtggc cggcgccctg cccgatcgcc ccgacgccca ccccgccttc ctgatcaccg
     2161 gctcgccggc cggggtctac gagcccctgc cctggatcgc gcctctgctc ggcttcctcc
     2221 gcgaggcgaa gggccggtcg aagctggtcg ggatctgctt cggccaccag gcgatggccg
     2281 aggcctatgg cggccgggtc gagaaatcgg agcgcggctg ggcggccggc ctccagcctt
     2341 atgaactggt cgcccggcga ccctggatgg gcgatcggcc gccggcgagg atcgccgtcc
     2401 cggtctcgca ccaggaccgg gtcgtcatcg ccccgccgga cgccgaggtg ctcgcccaca
     2461 gcccgttcag cccgttcggg atgctcgcct ggggaagcga cgcgctgtcg atgcagttcc
     2521 acccggagtt cgagcccgat tatgcgaaag ccctgatcga atatcgccgg gcccgcctcc
     2581 ccgcccccga cgccgccatc gcctcgctcg acgcccccga cgaccgggcc gtcgtggccg
     2641 ggtggataag ggcgttcctg aaaggaaatt cctcccctgc atttgcaggg gagggggacc
     2701 acccgaaggg tggtggaggg gcttctcccg acgcctgaag aacccctcca ccatgcttcg
     2761 catggtcccc ctccccgcga aatcgcggag aggaattgag gtcagcccgc cccgccgaag
     2821 gtgtcgcagg ccgccgggtc gcccgtctcc agcccgcgct tcagccactg gctgcgctgc
     2881 gccgagctgc cgtgggtgaa ggcctccggc accacccggc cctgggcctg gcgctggagg
     2941 gtgtcgtcgc cgatcgcctg ggcggctcgg agtccctcct cgacgtcgcc cggctccatc
     3001 accggccggc cctgggcgtc cttggcattg gcggcataga ccccggcata gcaatcggcc
     3061 tggagctcga cgcggacctg gatcgcattg ccttcggcgc gcgacacccg ccgctgctgc
     3121 gccgaggcct gctcgagcac cccggtcaga ttctggatat ggtggccgac ctcgtgggcg
     3181 acgacatagg cctgggcgaa gtcgcccgcc gccccgaact tgctctggag ctcgtcgaag
     3241 aagacggtgt cgaggtagat gcccctgtcg gtcgggcagt agaaggggcc catcgccgac
     3301 tgggccgcgc cgcagcccga gcggccgccg ccgccgtaga aggacagggt cggcggctgg
     3361 tacctctggc cgttggcggc gaacaatttc gcccaggtgt cctcggtgct cgccaggacc
     3421 tggcaggtga agcgcttggc cggctcgctg gagcaggcct gggcggcgtt gctcgtcgcg
     3481 cggccgccct ggtgcggcgc caccgccggg tccgggccgg tcaggccgat gccgccgcca
     3541 ccgcccatat agctgagcgc gcaatagccg agcagcagga tcagcactcc gccgatcccg
     3601 aagcggctga gcacgaaggg cagcagagcg aacaggcagc cgccgcctcc gccgccgccg
     3661 ccgaacccgc cgccgccgaa gccccgcccc tggtcgctga cgttgtcgct ggtgcggtag
     3721 cccccgagac gcattgccgc tctccctttg ttgcgaccga caatgatcgc cgccgctcat
     3781 ggttgcaaaa gcatcgcgac gccgcgaaag ggtccttcga tacgggcctt cgccaggctc
     3841 agtccctact caggatgagc gctggaggca tagctcctcc gctcatcctg agtagccgct
     3901 gagcgaagcc gaagcggcgt atcgaaggac gtatcgaagg acccccgcat gttcctcacc
     3961 ggcaagaccg ccctcgtcac cggctcgacc tccgggatcg gcctcgccgt cgcccgcgcg
     4021 ctcgccgccg aaggcgcccg cgtcaccctc aacggcttcg gcgagccagc cgagatcgag
     4081 cgcctcaagg aagagctggg cgccggctac agcggcgccg acctgaccag gtccgaggcg
     4141 atcgaggcga tgatggacga ggccggaccg gtcgacatcc tggtcaacaa tgccggcatg
     4201 cagcatgtcg ctccgatcga cgagttcccg gtcgagaaat gggaccttct gatcgccctc
     4261 aacctcaccg ccgccttcca caccatccga ctcgcgctgc cggcgatgaa gtcgaagggc
     4321 tggggccgcg tgatcaacac cgcctccgcg cacagcctcg tcgccagccc caacaaggcg
     4381 ccctacgtgg cgtccaagca cggcatcgcc ggcctcacca aggcggtggc gctggagacg
     4441 gcgacgaccg gcgtcaccgt caactgcatc agccccggct acgtctggac gccgctggtc
     4501 gagaaccaga tccctgacac gatgcgcgcc cggggcctca cccgcgagca ggtgatgaac
     4561 gacgtcctcc tctccgccca gccgaccaag aagttcgtca cggtcgacca ggtcgccgcc
     4621 atggccgtct tcctctgccg cgacgaggcg agcgcgatca cgggggccaa tctcagcatg
     4681 gacggaggct ggacggcggc ctgagatccc ccgggcttgc tcggggaggg ggtgatggag
     4741 gggtcgccag ggcggcccga tcggcgatgg acgccggatc cgcgctcagg cgaatctccc
     4801 caccacggcc tcgtggagcg gccggtcgaa gctgaggccc gcccgacccc cctccgccca
     4861 ggcgattcgc gcgggccagc tctccagccc cggcagcttg agccagacgc gcgatcccgg
     4921 ctcgcaatgg ccctccacct cgaggccgca gccatgggtc gacaggtcga tcaccgtcac
     4981 cggcgatccg ggccggtcgt tcgcgcgaga gaggccggcg ccgagatgga ccggatggcg
     5041 catgaagcgc cgccggtcct cggccagtgc tcgctccagc atggcggcaa cgcgcatgcc
     5101 gattcctcct tccgactcga cgggggtggc gggagtctcc ccccgcaagg ggtaaggagt
     5161 cgttaccggc ccggctggac agggccggga ccgcgctcta gcccaaggcg atgctgccgg
     5221 cgccgtccat catcgtcgaa tgggagaccg gccaggaagg ccatggcggc cgggccgcgg
     5281 cctgcctcgc cgagatcaac cgccagatgt gggaggcgcg caccgccttc gccgccccgc
     5341 ccgagctgat cctggtgttc gatccgcgcc aggcggcgcc cggctcggtc gaggcggcgg
     5401 ctgccgaggc ggcggccgga cgcggctggc ccggaaggtt cgagctcgtc gaggcgcccc
     5461 ccggatgcga ttattacggc aagaagaatc tcggcttccg ccgctcccgc ggcgacgtcg
     5521 ccctgttcgc ggattcggac ctggcgccgg agccgggatg gcttcgggcg ctggtcgagc
     5581 cgctcgccga cccggcccga agcgtcgtcg tcggccgcac ccatttcgag accgacaccc
     5641 tctacgagcg ggccatggcc ctgttctgga tcttcgacga gcggctcgac cagcattggg
     5701 tccggccgac tcggcggctc gtctccaaca acatcgcctt ccgccgggcc ctgttcgcgg
     5761 ccctgccctt cccggatcgt ccgacctacc ggggccagtg cagcgagctg ggcgcgacgc
     5821 tcgccgggct cggcatcgtc ctctac
//
LOCUS       DASVQZ010000324         9558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_263922, whole genome shotgun sequence.
ACCESSION   DASVQZ010000324 DASVQZ010000000
VERSION     DASVQZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263922"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF547_08995"
     CDS             <1..237
                     /locus_tag="VF547_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8262996.1"
                     /translation="GHQIWTAQEDLHGVAKALEASLGEAESMKLAWRPQTPVEVGGDD
                     AGTLIKLIDALEDDDDIQTVWGNYEVPDEVMERL"
     gene            234..719
                     /gene="ruvC"
                     /locus_tag="VF547_09000"
     CDS             234..719
                     /gene="ruvC"
                     /locus_tag="VF547_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX8262997.1"
                     /translation="MIIIGLDPGLGCTGWGVIAAEGNRLSHIGNGHIRTDPKAELPER
                     LVALHGELLSVIALHMPQAAAVEEVFVNDNPQSTLKLGQARGVVLLAAAGLGIAVGEY
                     AARLVKKAVVGTGGADKVQVHAMVQRLLPGVKIAGSDAADALAVAITHAHHLATASRV
                     A"
     gene            865..1461
                     /gene="ruvA"
                     /locus_tag="VF547_09005"
     CDS             865..1461
                     /gene="ruvA"
                     /locus_tag="VF547_09005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8262998.1"
                     /translation="MIARLRGLLDSFAADHAVVDVNGIGYLVFASTRTLSALGPVGGE
                     VVLHTVMQVSQDDIRLIGFASAEERDWFRLLVSVQGVGARVALAILSTLPGDDLHRAV
                     AAADSAMIARSPGVGPKLAQRIVHELKDKVGGLALGPASASAPAPGSAAADAVSALLN
                     LGFRPGEAASAVGKAEAELGPEASLDALVRTALKKAAR"
     gene            complement(1551..2678)
                     /locus_tag="VF547_09010"
     CDS             complement(1551..2678)
                     /locus_tag="VF547_09010"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8262999.1"
                     /translation="MDRREFLTASAATAALAALPAGALAQPPSTRPPSDPALWVDVQG
                     GVDGWDEPQPGRFVPSPKLIEAIRQRRIDVIGMTVGEVGNGPDRFRSAVQGIAEWDAL
                     IAQHAAHFMKIESAADIRAARAAGKLGLIYNFQDTTMLEGDPARVATFAALGVKQIQL
                     TYNKRNLAGDGCLERANAGLSDHGRDVIAEIEKAKVLLDLSHAGQRTIAEGIAAAKRP
                     LAITHSACRSLVDLPRNTFDSEMRALAEKGGVFGIYLMPFLRASGQPGKADLIRHIEH
                     AAKVCGEDHIGLGTDGPLFGYEINEETRKRHREFFEDRRKKGISAPGEAADVLNMVEG
                     YNDVGRYATLSADLKRRGWSQARIDKLLGGNFVRLFGEVWG"
     gene            2790..3806
                     /gene="ruvB"
                     /locus_tag="VF547_09015"
     CDS             2790..3806
                     /gene="ruvB"
                     /locus_tag="VF547_09015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8263000.1"
                     /translation="MEQDRIITGERRAEDVDAALRPKTLDDFVGQKAARENLRVFIEA
                     AKGRGDALDHVLFFGPPGLGKTTLAQIIARELGVGFRATSGPVIAKSGDLAALLTNLE
                     DGDVLFIDEIHRLAPAVEEILYPAMEDRALDLMIGEGPSARSVRIDLPKFTLVGATTR
                     QGLITTPLRDRFGIPVRLNFYSVEELEQVVRRAARLLGLALTEDGAKEIARRSRGTPR
                     IAGRLLRRVRDFAQSAGGEVDSKVADAALTRMEVDELGLDAMDRRYLTMIADLYKGGP
                     VGVETLAAGLSEPRDTIEEVIEPFLIQLGLVARTARGRCLNGKAWTHLGLTPPRTAQD
                     GLFD"
     gene            3968..5074
                     /locus_tag="VF547_09020"
     CDS             3968..5074
                     /locus_tag="VF547_09020"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX8263001.1"
                     /translation="MILALPSPATAHDGPHYERDSTWLCLPGRADTCGGPLATTALNA
                     NGYGSTGQVLPSKDAKADCFYVYPTISRDPGLNSDLVPGLEENATAAVQFARFGTVCR
                     TFAPIYRQVSLAALPRAFAGEDLSANFATAYGDVLEAWKNFLAHRNQGRPFVLIGHSQ
                     GSIHLLRLLAEEIEGKPEAKRMLSAILLGWPVEVPPGKKVGGSLKSTPLCSKVGQRGC
                     VVAYMSFRASSPPPADAFLGRAARAGMTAGCTNPAALGGGRAALDSYWFTLAPPQPGA
                     EPVVWSSQGNPPTPFLRTEGLVVAECRHSGTAGYLAITENADPKDARTDRIPGDVYVG
                     GKLNPGWGLHVADVNLAQGDLIRLVEAQVEGYSR"
     gene            complement(5071..5277)
                     /locus_tag="VF547_09025"
     CDS             complement(5071..5277)
                     /locus_tag="VF547_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8263002.1"
                     /translation="MADRLGNRLRELRAERDLTQAALAELVGVSRKTVNTIENGVFVP
                     STLLALKLARALGEPVEALFFLED"
     gene            complement(5270..5722)
                     /locus_tag="VF547_09030"
     CDS             complement(5270..5722)
                     /locus_tag="VF547_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263003.1"
                     /translation="MTKTEDVEQADRLSRRRMRMVPGLAAIFLIQQASYFSGQIEDGA
                     RTVDHVKIAAWLVLSIVLLLFVTTGGFWFHSRRVRALLNDEATRANRLEGMRIGFIAA
                     MAAGIALYFVALFEPLSGRDAIHLLMTAGIAAALLRFGQLERRDHRDG"
     gene            5895..6317
                     /locus_tag="VF547_09035"
     CDS             5895..6317
                     /locus_tag="VF547_09035"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEX8263004.1"
                     /translation="MRGSTHLFPLRVYFEDTDVAGIVYYANYLRFIERARSDMLRLIG
                     VDQRGALEGGEGVYAVAELAVKYRAPARLGDDLVVATEIESVRAASVLIHQRVMRADQ
                     LLADARVTAAFLTPDGRPRRQPREWVEAFERLLGEGRE"
     gene            6329..7003
                     /gene="tolQ"
                     /locus_tag="VF547_09040"
     CDS             6329..7003
                     /gene="tolQ"
                     /locus_tag="VF547_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX8263005.1"
                     /translation="MTGADMSPVALFLQADVVVKAVIVGLLLASIWTWAIIVGHALRL
                     RRASNGSRDFERDFWKADDIDRFYETRGQTDLPSAKVLAAGIAEWRRSTGGKVVDREG
                     ARSRLALVMHSALTEEVDKLAVRLNILATVGSVAPFVGLFGTVWGIMRSFTAIAGANN
                     TSLAVVAPGIAEALFATALGLFAAIPAVIAYNRMSFGINRLEARLQRFADGFHATLSR
                     ELELEG"
     gene            7158..7598
                     /locus_tag="VF547_09045"
     CDS             7158..7598
                     /locus_tag="VF547_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8263006.1"
                     /translation="MAMGPILGSHRRARRSPMAEINVTPMVDVMLVLLIIFMVTAPLL
                     VAGVPVDLPDSKAGALDQERKPVQISLEADGSLFLDDQPVPATELGPRLAGIAASSRE
                     RGGPRIHLRADRSLDYGRVMAVMGEINAAGLRRVALVTTQGSER"
     gene            7598..8380
                     /locus_tag="VF547_09050"
     CDS             7598..8380
                     /locus_tag="VF547_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEX8263007.1"
                     /translation="MDRAEAAGLGVATLGHAALLAALTYGFAASRLPVPKSDPIEVSF
                     VDEVGLESRSPTPSAAEPAPLPGPPDAAPQPAMPAPPRLPEVPAARPEPRLPPPGLAP
                     AAKARPSPAPPKAPAIPARPASRRDAGGLSSVVAGLSDRANPSQSATPPAAAAGAAAQ
                     ASLRAEVRRQLKPHWKAPTGADAEQLRTELSISLARDGRVTRVEVIRTTGQTASNRPQ
                     VKLHQERAMRAVQLASPFNLPAEYYEAWKELSPIGFDKRLSQ"
     gene            8380..>9558
                     /gene="tolB"
                     /locus_tag="VF547_09055"
     CDS             8380..>9558
                     /gene="tolB"
                     /locus_tag="VF547_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX8263008.1"
                     /translation="MKRLFPLVLLAFAFPASAQLSVDVTDESGDDLVIAVPVMPTPQV
                     ADTPAGTTEALGRQVAEVVAADLRGSGLFRPVGPGGVRSVPFAEVAAPAYEAWSGAGA
                     AALVQGFVRANADGQLTVGCYLYDVALRSELTRQGYVVAPRDWRRAAHKCADAIYSRL
                     SGESPFFDSRIAYIAESGPKGARVKRLAIMDSDGANHRFLTNGQSTVLTPRFSPDYKQ
                     IVYLSYVEKRPRIYIYDIGTGRQRVVTESSNQTFAPRFSPDGRWILYAMSVNGNTDIY
                     RVSAQGGTPVRLTNSPGIDVGGSYSPDGSRIVFESDRSGSQQLYVMNADGSNQRRISF
                     GGGGYATPEWSPRGDLIAFTRIAGNFRVGVMSPDGSGERLLTDSWQDEAPSWSPNGRV
                     L"
BASE COUNT         1402 a         3490 c         3231 g         1435 t
ORIGIN      
        1 ggccaccaga tctggacggc gcaggaggat ctgcacgggg tcgccaaggc gctggaggcg
       61 agcctgggcg aagcggagag catgaagctc gcctggcgcc cgcaaacgcc ggtcgaggtc
      121 ggcggcgacg atgccgggac gctcatcaag ctgatcgacg ctctcgagga cgacgacgac
      181 atccagaccg tctggggcaa ttacgaagtg cccgacgagg tgatggagcg gctttgatca
      241 tcatcggcct ggatcccggg ctcggctgca cgggctgggg cgtgatcgcg gctgagggga
      301 accggctgag ccatatcggc aacggccata tccgcaccga tccaaaggcc gagcttccgg
      361 agcggctggt ggcgctccac ggtgagctgc tttcagtaat tgcgcttcac atgcctcagg
      421 cggctgcggt ggaggaggtg ttcgtcaacg acaacccgca atcgaccctg aagctcggcc
      481 aggcgcgcgg ggttgtgctg ctcgccgccg ccgggctcgg catcgccgtc ggcgaatatg
      541 cggcccggct ggtcaagaag gcggtggtcg gcaccggcgg cgcggacaag gtccaggtcc
      601 acgccatggt ccagcgactt ctgcccggag tgaagatcgc cggctccgac gcggccgacg
      661 ccctcgccgt cgccatcacc cacgcccatc atctggcgac ggcgagccgg gtggcctgag
      721 gggcgagacg gccgaccccc caaccgttcg gcccgggcct gtcgaaggcc cttcctttgt
      781 tcgtcggaag cgaaggcgag ggcttcgaca agctcagccc gaacggataa ggtccggaat
      841 gcccatcgtc cacccggccc gcccatgatc gccaggctcc gcggcctcct cgactcgttc
      901 gccgccgacc acgccgtcgt cgacgtcaac ggaatcggct atttggtctt cgcctcgacc
      961 cggacgcttt cggcgctcgg accggtcggc ggcgaggtcg tcctccacac ggtgatgcag
     1021 gtctcacagg acgacatccg gctgatcggc ttcgcctcgg ccgaggagcg cgactggttc
     1081 cggctgctcg tgtccgtgca gggcgtcggc gccagggtcg ccctcgccat cctctcgacc
     1141 cttcccgggg acgatctcca ccgcgccgtc gccgccgccg attcggcgat gatcgcgcgc
     1201 tcgcccggcg tcggccccaa gctcgcccag cgcatcgttc acgagctcaa ggacaaggtc
     1261 ggcgggctcg ccctcgggcc ggcgtcggcc tcggcgccgg cgcccggcag cgccgccgcc
     1321 gacgccgtct cggcgctcct caacctcggc ttccgccccg gcgaggcggc ctccgccgtc
     1381 ggcaaggccg aagccgagct cggccccgag gcaagcctcg atgcactggt gcgaacggcg
     1441 ctcaagaagg cggcgcgcta gccgtcattg cgagcgaagc gaagcaatcc acggcagcgt
     1501 cggacgcaac agctggattg ccgcgtcgct gcgctcctcg caatgacggg ctacccccag
     1561 acctcgccga acagccggac gaaattgccg cccagcagct tgtcgatccg cgcctggctc
     1621 cagccccgcc gcttgaggtc ggcggagagc gtcgcgtagc ggccgacgtc gttatagccc
     1681 tcgaccatgt tgagcacgtc ggccgcctcg ccgggcgcgg agatgccctt cttcctgcgg
     1741 tcctcgaaga attcgcggtg gcgcttcctc gtctcctcgt tgatctcgta gccgaacagg
     1801 ggcccgtcgg tgccgaggcc gatatgatcc tcgccgcaca ccttcgcggc atgctcgatg
     1861 tggcggatca ggtccgcctt gccgggctgg ccgctcgccc tcaggaacgg catcaggtag
     1921 attccgaaca cgccgccctt ctcggccagg gcccgcatct cggagtcgaa cgtgttgcgc
     1981 ggcaggtcga cgaggctccg gcaggcgctg tgggtgatcg cgagcgggcg cttcgccgcg
     2041 gcgatgccct cggcgatcgt cctctggccg gcatggctga ggtcgaggag gaccttggcc
     2101 ttctcgatct cggcgatcac gtcccggccg tggtcggaga ggcccgcatt ggcccgctcg
     2161 aggcagccgt cgccggcgag gttgcgcttg ttgtaggtga gctggatctg cttgacgccc
     2221 agcgcggcga aggtggccac ccgcgccgga tcgccttcca gcatcgtcgt gtcctggaag
     2281 ttgtagatga ggccgagctt gccggcggcg cgggccgccc ggatgtcggc ggcgctctcg
     2341 atcttcatga aatgcgcggc atgctgggcg atcagggcgt cccactcggc gatcccctga
     2401 accgcgcttc ggaaccggtc ggggccgttg ccgacctcgc ccaccgtcat gccgatgacg
     2461 tcgatccgac gctgcctgat cgcctcgatc agcttggggg agggcacgaa gcggccgggc
     2521 tgcggctcgt cccacccgtc gaccccgccc tggacgtcga cccacagggc cggatcggaa
     2581 ggggggcggg tcgagggcgg ctgggcgagg gctccggcgg ggagggcggc gagggccgcg
     2641 gtcgctgcgc ttgcggtcag gaattcgcgg cggtccatgg gcgctccttt gcctgctccc
     2701 gtctccgacg gggagggcga gcctagcccc tcatccgcgg ccgataaagc cgctcgacag
     2761 caagggcgct tcggctaaag ctcggccccg tggagcagga tcggatcatc accggagagc
     2821 ggcgggccga ggatgtcgac gcggcgcttc gccccaagac gctcgacgat ttcgtcggcc
     2881 agaaggccgc ccgtgagaat ctgcgcgtgt tcatcgaggc cgccaagggc cgcggcgacg
     2941 cgctcgacca tgtcctcttc ttcggcccgc cgggcctggg caagacgacg ctcgcccaga
     3001 tcatcgcccg cgagctcgga gtcggcttcc gggccacgtc gggcccggtc atcgccaagt
     3061 ccggcgatct cgccgccctt ctcaccaatc tcgaggacgg cgacgtcctg ttcatcgacg
     3121 agatccaccg gctcgcgccc gccgtcgagg agatactcta tccggcgatg gaggaccggg
     3181 cgctcgacct gatgatcggc gagggccctt cggcccgatc ggtccgcatc gatctcccca
     3241 agttcaccct cgtcggagcg accacccgcc agggcctgat cacgacgccc ctgagggacc
     3301 ggttcgggat tcccgtccgc ctcaatttct attcggtcga ggagctcgag caggtcgtcc
     3361 gccgggccgc ccggctgctc ggcctggcgc tgaccgagga tggggcgaag gagatcgccc
     3421 gccgctcgcg cgggaccccg cgaatcgccg gccggctgct ccgccgggtg cgcgatttcg
     3481 cccagtccgc cggaggcgag gtcgattcga aggtcgccga cgccgctttg acccggatgg
     3541 aggtggacga gctcggcctc gacgcgatgg accggcgcta cctcaccatg atcgcggacc
     3601 tctacaaggg cggcccggtc ggggtcgaga cgctcgccgc cggcctctcc gagccccgcg
     3661 acacgatcga ggaggtgatc gagcccttcc tcatccagct cggcctcgtc gcccgcaccg
     3721 cccgcggccg ctgcctcaac ggcaaggcct ggacccacct cggcctgacc ccgcccagga
     3781 cggcgcagga cggcctgttc gattgaattg gagagagcgc aaaatgaatc cgctgatgga
     3841 aaaatctccg ttcgccctga gcctgtcgaa gggcctttct ttcctttcga acgcaacggg
     3901 gaagaggggg gcttcgacag gctcagcccg aacggctcgg cgaacggctc tcctggtggg
     3961 cttgctgatg atcctcgcgc tcccttcacc cgcgaccgcc catgacggcc cgcattacga
     4021 gcgggattcg acctggctct gcctgccggg ccgggccgac acctgcggcg ggcccctggc
     4081 gaccaccgcg ctcaacgcca acggctacgg ctccaccggc caggtgcttc caagcaagga
     4141 cgcgaaggcc gactgcttct acgtctaccc gaccatctcg cgcgatccgg gcctcaacag
     4201 cgacctcgtc cccggcctcg aggagaatgc gaccgcggcg gtccagttcg cccgcttcgg
     4261 aacggtctgc cggaccttcg ctccgatcta ccgccaggtc agcctcgccg ccctcccgcg
     4321 cgccttcgcc ggcgaggacc tcagcgccaa tttcgcaacc gcttacggcg acgtgctcga
     4381 ggcctggaag aacttcctcg cccaccgcaa ccagggccgg cccttcgtcc tgatcggcca
     4441 cagccagggc tcgatccacc tgctcaggct gctcgccgag gagatcgagg gcaagccgga
     4501 ggcgaaaagg atgctctcgg cgatcctgct cggctggccg gtcgaggtgc cgccgggcaa
     4561 gaaggtcggc ggcagcctga agtcgactcc gctctgcagc aaggtcggcc agcgcggctg
     4621 cgtcgtcgcc tacatgtcgt tccgggcgtc gagcccgccg ccggccgacg ccttcctcgg
     4681 gcgggccgcg cgcgcgggga tgaccgcggg ctgcaccaac cccgcggctc tgggcggcgg
     4741 ccgagccgcg ctcgattcct actggttcac cctcgcgccg cctcagccgg gggccgagcc
     4801 ggtggtctgg tcgagccagg gcaatccccc gacgcccttc cttcgcaccg aagggctggt
     4861 tgtcgccgaa tgccggcaca gcggcactgc cggctatctc gcgatcacgg aaaatgccga
     4921 tcccaaggac gcgcgcaccg accgcatccc cggcgacgtc tatgtgggcg gcaagctcaa
     4981 cccgggctgg ggcctgcacg tcgccgacgt caatctcgcc cagggcgacc tcatccggct
     5041 cgtcgaagcg caggtggagg gctattcgcg ctagtcctcc aggaagaaga gcgcctcgac
     5101 cggctcgccc agcgccctcg cgagcttcag ggccagcagg gtggagggga cgaaaacgcc
     5161 gttctcgatc gtattgacgg tctttcggct gactccgacc agctcggcca gcgccgcctg
     5221 ggtgaggtct cgctcggcgc ggagctcgcg cagccggttg ccgaggcggt cagccatcgc
     5281 ggtggtcgcg ccgctcgagc tggccgaatc gaaggagtgc ggccgcaatc cccgcggtca
     5341 tcaggaggtg gatggcgtcg cgtccgctga gcggctcgaa gagggcgacg aagtacaagg
     5401 cgatccccgc cgccatcgcc gcgatgaagc cgatcctcat cccctcgagc cggttcgcgc
     5461 gggtcgcctc gtcgttgagc agcgctcgaa cgcgcctgga atggaaccag aagccgccgg
     5521 tcgtgacgaa cagcaggagg acgatcgaca ggacgagcca ggccgcgatc ttcacatgat
     5581 cgaccgtcct cgccccgtcc tcgatctggc ccgagaaata gctggcctgc tggatgagga
     5641 agatcgcggc cagccccggc accatccgca tacggcggcg gctcagccgg tccgcctgct
     5701 ccacgtcttc agtcttcgtc atcgcccgct cctgagtaac ctataggtga catgtaacct
     5761 caaggttaca ggtcgtcaac gcaaaatcgt cacgcctcgt cccggcgggg ccctttgggg
     5821 ttgcggcggc gaatccgcag ccgatagagc cgtaggcgat gggcggggac ggaccgaagc
     5881 cggcggacgg cgtcatgcgc ggcagcacgc acctcttccc gctgcgcgtc tatttcgagg
     5941 acaccgacgt cgccgggatc gtctattacg ccaactacct gcgcttcatc gagcgcgccc
     6001 gctccgacat gctccggctg atcggggtcg accagcgcgg cgcgctcgaa gggggcgagg
     6061 gcgtctatgc ggtggccgag ctcgccgtga aatatcgcgc cccggcaagg ctcggcgacg
     6121 acctcgtcgt cgccaccgag atcgaatcgg tacgcgcggc gagcgtgctc attcatcagc
     6181 gggtcatgcg cgccgatcaa cttctggccg acgccagggt cacggctgcc tttttgactc
     6241 cggacggccg cccccggcgt cagccgcgcg agtgggtcga ggcgttcgag cggttgcttg
     6301 gggaaggaag agagtgaacg acttggcgat gacgggagcc gacatgtcgc cggtcgccct
     6361 gttcctgcag gcggacgtcg tggtgaaggc cgtgatcgtg ggcctgctcc tggcgagcat
     6421 ctggacctgg gcgatcatcg tcggccacgc gctgaggctc cggcgcgcgt ccaacggatc
     6481 gagggacttc gagcgcgact tctggaaggc cgacgacatc gaccgcttct acgagacccg
     6541 cggccagacc gacctgccga gcgccaaggt gctcgccgcc ggcatcgccg agtggcgccg
     6601 ctcgaccggc ggcaaggtgg tcgaccgcga aggcgcccgc tcgcgtctcg ccctggtcat
     6661 gcattcggcc ctgaccgagg aggtcgacaa gctcgccgtc cggctcaaca tcctcgccac
     6721 ggtcggatcg gtcgccccct tcgtcggcct gttcggcacg gtctggggga tcatgcgcag
     6781 cttcaccgcg atcgccggcg ccaacaatac cagcctcgcc gtcgtcgctc ccggcatcgc
     6841 cgaggcgctt ttcgccaccg ctctcggcct gttcgcggcg atcccggcgg tgatcgccta
     6901 caatcgcatg agcttcggca tcaaccgcct cgaagcgcgc ctgcagcgct tcgccgacgg
     6961 cttccacgcg accctgtcgc gcgagctgga gctggaggga tgaggggagt gcagccgcag
     7021 ctcctccccg gaacggggag ggggaccatg cgaagcatgg tggaggggcc ccgcctcctc
     7081 gccgcgccag cgccgggccc cctccaccac gccgcttcgc gacgcggtcc ccctccccgt
     7141 tccggggagg atgattgatg gcgatgggtc ccatcctcgg ctcgcaccgc cgggcccgcc
     7201 gctcccccat ggcggagatc aacgtcacgc cgatggtcga cgtgatgctg gtcctcctga
     7261 tcatcttcat ggtcacggcg ccgctcctcg tcgccggggt cccggtcgac ctgcccgaca
     7321 gcaaggccgg agcgctcgac caggagcgca agcccgtcca gatctcgctc gaggccgacg
     7381 gctccctgtt cctcgacgac cagcccgtgc cggcgacgga gctcggcccg cggctcgcgg
     7441 gcatcgcggc ctcctcgcgc gagcggggcg gtccccgcat ccaccttcgc gccgaccgct
     7501 cgctcgacta cggccgggtg atggcggtga tgggcgagat caacgccgcc ggcctgaggc
     7561 gggtcgcgct ggtcaccacc cagggaagcg agcgctgatg gaccgggcgg aagccgccgg
     7621 gctgggcgtc gcgacgctgg ggcatgcggc cctgctggcg gcgctcacct acggcttcgc
     7681 ggcgtctcgc ctgccggttc cgaagagcga cccgatcgag gtctcgttcg tcgacgaggt
     7741 cggtctcgag agccggtcgc cgacgcccag cgccgccgag ccggcgccgc tccccgggcc
     7801 gccggatgcc gccccgcagc ccgcgatgcc ggccccgccc cggctcccgg aggttcccgc
     7861 ggcgcgcccc gagccgcgcc tgccgccgcc gggcctcgcg ccggcggcca aggcccggcc
     7921 gagcccggcg ccgcccaagg cgccggccat ccccgccagg ccggcttcgc gacgggacgc
     7981 cggcgggctg agcagcgtcg tcgccggact aagcgatcgg gccaatccga gccagtcggc
     8041 cacgccgccc gcggccgccg ccggcgccgc cgcccaggcc tcgctccggg ccgaggtccg
     8101 ccgccagctc aagccgcact ggaaggcgcc gaccggtgcc gacgcggagc aattgcgaac
     8161 cgagctgagc atctcgctcg cccgcgacgg gagggtcact cgggtcgagg tgatccgcac
     8221 caccggccag accgccagca accgccccca ggtcaagctc caccaggagc gcgcgatgcg
     8281 ggcggtgcag ctggcgtccc ccttcaacct gcccgccgaa tattacgagg cgtggaagga
     8341 gctcagtccc atcggtttcg acaagaggct ctcgcaatga tgaagcgcct gttcccgctc
     8401 gtcctgctcg ctttcgcttt tccagcctcg gcgcagctca gcgtcgacgt caccgacgag
     8461 agcggcgacg atctcgtcat cgccgtcccg gtgatgccga ccccccaggt cgccgacacg
     8521 ccggccggga ccaccgaggc gctcggccgc caggtcgccg aggtcgtcgc cgccgacctt
     8581 cgaggaagcg gcctgttccg ccccgtcggc cccggcggcg tgcgctccgt cccgttcgcg
     8641 gaggtcgccg cgcccgccta cgaggcctgg agcggcgccg gcgccgcggc cctcgtccag
     8701 ggcttcgtcc gcgccaatgc cgacggccag ctcaccgtcg gctgctacct ctacgacgtc
     8761 gccctccggt ccgagctcac ccgccagggc tatgtcgtcg ccccgcgcga ctggcgccgg
     8821 gccgcgcaca aatgcgccga cgccatctat tcgcgcctgt cgggcgagag ccccttcttc
     8881 gacagccgga tcgcctacat cgccgagagc ggccccaagg gcgcccgggt gaagcggctc
     8941 gcgatcatgg attccgacgg cgccaaccac cgcttcctga ccaacggcca gtccaccgtg
     9001 ctgaccccgc gcttctcgcc cgactacaag cagatcgtct atctgagcta tgtcgagaag
     9061 cggccgagga tctacatcta cgacatcggc accggccgcc agcgggtggt gaccgagagc
     9121 tcgaaccaga ccttcgcgcc gcgcttctcg cccgacgggc gctggatcct ctacgccatg
     9181 tcggtcaacg gcaataccga catctaccgg gtctccgcgc agggcggcac tccggttcga
     9241 ctgaccaact cgccgggcat cgacgtcggc ggctcctact cgccggacgg cagccggatc
     9301 gtgttcgaga gcgaccggtc gggcagccag cagctctacg tgatgaacgc cgacggctcg
     9361 aaccagcgcc ggatcagctt cggcggcggc ggctatgcga cgcccgaatg gagcccgcgc
     9421 ggcgatctca tcgccttcac ccggatcgcc ggcaactttc gggtgggagt gatgagcccg
     9481 gacggctcgg gcgagcggct tttgaccgac agctggcagg acgaggctcc gagctggtcg
     9541 cccaacggcc gggtcctt
//
LOCUS       DASVQZ010000325         3160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_743843, whole genome shotgun sequence.
ACCESSION   DASVQZ010000325 DASVQZ010000000
VERSION     DASVQZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743843"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..405
                     /locus_tag="VF547_09060"
     CDS             31..405
                     /locus_tag="VF547_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263009.1"
                     /translation="MRHWLALAAAAASCLATAPAAARAGAERRLSDADLLAYASQRFD
                     KRAMMFKRVALGRHRGLPVVAIHPCGDVCPVYTRRIVHYDIPLADCARRGGVAVERIM
                     PRGPGIARQPFCVPAVLASKTP"
     gene            409..1050
                     /locus_tag="VF547_09065"
     CDS             409..1050
                     /locus_tag="VF547_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263010.1"
                     /translation="MPVFVHLTSHRNLRSIRRGGIRAGRSAGVHALPVTRNFQISHQW
                     LRELRRGGGGTVFGVYFRIRDDEPVEIGHYHRPHRPMTAAEAVAVMLEAESNDPGAAR
                     VADSGSRAIARGRVLPSSPEGFEVLIPRPIAPAEILRVKALPQVVGWRYRPGANGAAP
                     SACLWAERGVWGIRRLERAVERAEAAGRPAPATLFTRDQASFRRVERLRRGRG"
     gene            complement(1492..1791)
                     /locus_tag="VF547_09070"
     CDS             complement(1492..1791)
                     /locus_tag="VF547_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8263011.1"
                     /translation="MKVRLADEAEVDLEAIGDEIAKDNPARAASFIHELRERCLGLAR
                     YPKRFPLVPRYERRGVRHCVHGNYLIFYRVDVEAVIVIHVLHGAMDYSRILFPSE"
     gene            complement(1788..2045)
                     /locus_tag="VF547_09075"
     CDS             complement(1788..2045)
                     /locus_tag="VF547_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX8263012.1"
                     /translation="MISADLGRQLESFVAELVDTGRYNSKSEVLREGVRLIHERETRL
                     AALDASIRRGLADADEGRTKPAADVFDRLETKYRGMIRPAR"
     gene            complement(2279..2647)
                     /locus_tag="VF547_09080"
     CDS             complement(2279..2647)
                     /locus_tag="VF547_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263013.1"
                     /translation="MVGGIFLESDAGKVRWLDIGAGLAEDVADSVEAFEAILRSDAPV
                     VEEWFLPGLVEALYEAGKRPGPGEAFWFIIPPVFAEGRYSADNMAIVPIREQLLASAD
                     LHRQLADLPEGATVRMKVVD"
BASE COUNT          474 a         1174 c         1028 g          484 t
ORIGIN      
        1 gcccgaaata tgaatcgagg aggatcgacg atgcggcact ggctggccct ggcggcggca
       61 gcggcttctt gcctggcgac ggcgcccgcc gcggcgcgcg cgggcgccga gcgccggctg
      121 agcgacgccg accttctggc ctacgcctcg cagcgcttcg acaagcgcgc gatgatgttc
      181 aagcgggtcg cgctcggccg gcaccgcggc ctcccggtcg tcgccatcca tccctgcggc
      241 gacgtctgcc ccgtctatac gcggcgcatc gtccattacg acattccgct ggccgattgc
      301 gctcggcggg gcggggtcgc cgtcgaacgg atcatgccga gagggccggg catcgcccgc
      361 cagcccttct gcgttccggc cgtcctcgcc tcgaagacgc cctaggcgat gccggtcttc
      421 gtccacctca cctcgcaccg caacctgcgg tccatccgcc gcggcggcat ccgcgccggg
      481 cgctcggccg gggtccatgc ccttcccgtc acccgcaact tccagatctc gcaccagtgg
      541 ctgcgcgagc tgcgccgggg cggcggcggc accgtcttcg gcgtctattt ccggatccgg
      601 gacgacgaac cggtcgagat cggccattac caccggcccc accggccgat gaccgcggcc
      661 gaggccgtcg ccgtcatgct ggaggcggag tcgaacgatc ccggcgccgc ccgagtcgcc
      721 gattccggct cgcgggcgat cgcccgcggc cgcgtcctgc cctcgtcgcc ggaaggcttc
      781 gaagtgctga tcccgcggcc catcgcgccg gccgagatcc ttcgggtgaa ggcgctgccg
      841 caggtggtcg gctggcggta tcggcccggc gccaacggcg ccgcccccag cgcgtgcctc
      901 tgggccgagc gcggagtctg gggcatccgc cggctcgagc gcgccgtgga gcgggccgaa
      961 gcggccggcc ggcccgcccc ggccaccttg ttcacccgcg accaggcctc cttccgccgg
     1021 gtcgagcggc tgaggcgcgg ccgcggctga cgccccggct gccggcgccg ccgacgcgcg
     1081 cgcggcggga acgtcgtgat gctcgcctcc gcacggtccg gtgagagcag ccgtgtggaa
     1141 cgggaaggac gggtgccggc acctgttccc cgcccctggc gcaacatgtt ggccctcacg
     1201 cggcgacgga gccggaaact aacggtttcg cgcagagccg caaagccgcg aagagggggc
     1261 cggccgatgc ttcccccacg atctccttcc gagcggaacc gggagcccac tgcgtcggcg
     1321 cttcgatcgg caccttcgcc ccgtcggccc ccgtcttcag cggcgccgcc acccggccgc
     1381 cgccgccgag gcgcccccgc tcatccggcg ccgctactgc caggcacgtg cccggcacgt
     1441 ggctggtacg tgcctggcac cgaaactccg cggcgcgggc cgcggtcgcc ttcattcgga
     1501 ggggaacagg atccgcgaat aatccatcgc gccatgcagt acatggatca cgatcacggc
     1561 ctcgacgtcc acccgataga agatcagata gttgccgtgg acgcagtggc ggactccgcg
     1621 ccgctcgtag cggggtacca gcggaaagcg cttcgggtag cgcgccaggc cgaggcatcg
     1681 ctcccgcaac tcatggatga agcttgccgc acgcgccgga ttgtccttcg cgatctcgtc
     1741 gccgatggct tcgaggtcga cctcggcttc atcggcaagg cggactttca ccgcgcgggc
     1801 cggatcattc cccgatattt ggtctcgagc cggtcgaaca cgtccgcggc cggcttcgtg
     1861 cggccctcgt ccgcgtcggc gaggccccga cggatcgaag cgtccagagc cgccaggcgc
     1921 gtctcccgct cgtggatgag gcggacgccc tcgcgcagca cctcgctctt gctgttgtag
     1981 cggccggtat cgaccagttc ggccacgaaa gactccagct gccgtccaag gtccgcgctg
     2041 atcatggcat tctccgaatg cgtgactgag acagctttta tcaactatta ccaaggccgg
     2101 caaggcgagg ccagccccct gtgctgagga gtcgctggtg ccagctacgt gcctggcacc
     2161 aacccttacc atctacgtgc cagctacgtg cctggcaccg gtaggcccaa cctcacgcgc
     2221 ccctcgccga cgcggctatt gccgcggagc gtcaccgagg ccgacggcgg acaccgcctc
     2281 agtccacgac cttcatcctc accgtcgccc cttcaggcaa atccgcgagc tgcctgtgga
     2341 ggtccgcgct cgccaggagc tgctcccgga tcggcacgat cgccatgttg tcggccgagt
     2401 atcggccctc ggcgaagacc ggcgggatga tgaaccaaaa ggcctccccc ggcccgggcc
     2461 gcttccccgc ctcgtaaagc gcctcgacca ggccggggag aaaccattcc tctacgacag
     2521 gcgcatcgga tcggaggatc gcttcgaacg cttcgacgct atccgcgaca tcttccgcga
     2581 ggccggcacc gatatcgagc catcggacct ttccggcgtc gctttccagg aaaattcccc
     2641 caaccattgc gcagacgagg acacgccacg gggccggcac cagccaggac caggccgccc
     2701 cggtctccct ggcaagctcg ggatcaggga cgaaagcgat gtcgttccaa ccgacgggca
     2761 tacctcatgc tactcggcat tttctcaccc gtcatctcgc aagccgtcgc gctcgcggcg
     2821 cgggcagcgc ttggctcaac cgtcgatcgg cgcgaaaata gtgacggaaa aatagtgaca
     2881 gtatacgatt gtccccggag tggacgggcc tcgcctaaga gccgctggtg ccaggcacgt
     2941 gcctggcacg tagctggtac gtgcctggca ccgaaactca cgtagctggt acgtgcctgg
     3001 caccgaaact ccccgggccc gccgtcattg tggcgcgcgg gcgcggtcct caggctcccg
     3061 gcgccccgcc cgaacacgct cggctcaatt cttcctgggc ccgtttcgcg accgacgcgc
     3121 cgctgctagt cgttcgaaaa atcggctgcg gccagagggg
//
LOCUS       DASVQZ010000326         9602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_169581, whole genome shotgun sequence.
ACCESSION   DASVQZ010000326 DASVQZ010000000
VERSION     DASVQZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169581"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1743)
                     /locus_tag="VF547_09085"
     CDS             complement(88..1743)
                     /locus_tag="VF547_09085"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEX8263014.1"
                     /translation="MPRVLVAFLLILSGCASAREAAPAYDTILRGGTVYDGSGAAPFV
                     GDVAIRGDRIAAIAPRIPGKARQEIDVRGLAVAPGFINMLSWAAEPLIRDGRAQSDVR
                     QGVTLEVMGEGWSMGPLSPAQKAEALAEQTTTRYPISWTTLGEFLAFLERKGVSVNVA
                     SMVGASTIRVHELGRNDVDPTPEQLARMRALVRRAMDEGAMGVGSSLIYVPAIYAETD
                     ELVALAAEAGRCGGMYISHMRSESSGLLPAIDELVEISRRSGARAEIYHLKAVGRSNW
                     PLMDAALERIEAARAAGVPVTADMYLYTASGTGLDSTLPIWVREGGLRAMIDRLKKPE
                     LRARVVAEMQGGSRDWNTVQPVGFANPALRVHAGRRLPEIAARRGKSAEETAVDLIVE
                     DDSAVGTIFHSISEDNLRKVLAKPWVSFGSDAAAIAAEPPFTDKATHPRAYGNFARLL
                     GRYVRDEKALSLAEAVRRLTSLPADNLRLADRGRLRPGFHADIAVFDPATIADRATYE
                     ESHRYAVGMRHVLVNGVAVLRDGEHTGAKPGRFVKGPGWRKCR"
     gene            complement(1940..3628)
                     /locus_tag="VF547_09090"
     CDS             complement(1940..3628)
                     /locus_tag="VF547_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX8263015.1"
                     /translation="MTYDFLIEMGWKSAAIAGGALLLAALLRSRAAADRGAVLKVAVA
                     LLLALPAIALFAPALEIETAAPAAAAPAPAAPAEAAAFEPLAAAPASATSQSPALQGP
                     SDNWDDPSLLFFLLYAGGVAMVGGRLLAGLMTLRRWTRAAREIEAPEWRSALTRAGGD
                     SLGIRLLASEDAVSPLSWGLRRPVILLDRDTLRSPAEADAILVHEVAHVARRDWPSLI
                     LSRLAVALFWFNPLVWRLDREVAQQAEEAADSDAAAAVEPARYAQTLLDWARLSGANA
                     LPANAIAAGEPGLSKRVRAILDGRVSRRSGSFWAVAAMLGCAAFAAPVAALDFVEGVP
                     EAPAAPAAPAAPPSPHSAVAPMAPMAPMAAPAPIAAAFVPPQPGAAPSAAPTPVPPLP
                     VIAVRPPRVPAPPQVPHAAIAAAVRASAHARAAAHASAGEVDEAAIEAEVAAAVAEAS
                     RAIEAQSAEIGRTVERALANAKAGMINGARGMENGAAGMERGAAHMQAEARRLRDPAY
                     REQKIREHAARGKRLTHEDLLEAAEGMEEGAEGMREGAREMREAAAEMRRGRHD"
     gene            complement(3625..3999)
                     /locus_tag="VF547_09095"
     CDS             complement(3625..3999)
                     /locus_tag="VF547_09095"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8263016.1"
                     /translation="MIASLPRREREIFEILCSAGEATAADVRRAMADPPSHSAVRTML
                     ARLEAKGLVRHRADDQTYVYRSVPQPAKVRESALKALVKTFFDGSAASAATALLGLSK
                     SLKPDELDALQRAIDEAKERDQ"
     gene            complement(4320..5087)
                     /locus_tag="VF547_09100"
     CDS             complement(4320..5087)
                     /locus_tag="VF547_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8263017.1"
                     /translation="MRATFWLIMALATAAPAAAMAPARAPADVAAAIAAPGRPKAATD
                     LDAVRKPAEVLRFMGLKKGDRVLDYFTGTGYYADIIAKAVGPRGHVVGWNSASFARRE
                     PVVKALADIRARNPNTAFYSSPSTAIAFAPSSFDFALLHLVYHDAYWESAQFGLPRID
                     PSTVVQAIWHAVKPGGTVAVIDHVATAGGDTREVVEKLHRIDPAVVRADFERAGFVLE
                     AQSDILRVAEDDHSKNVFDPSIRGKTDRFVYRFRKPR"
     gene            complement(5108..6700)
                     /locus_tag="VF547_09105"
     CDS             complement(5108..6700)
                     /locus_tag="VF547_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8263018.1"
                     /translation="MSLTRRALLERLGLAGGVSAVYLGMEAMGLLNAPPAAAEPFELP
                     PPPGKRKSVVVLGAGIAGLVAAYELRRAGWHVTVLEARDRIGGRVWTLRGGDRIEQIG
                     RPDQVCGFSKGLYLNAGAARIPTAHHTILGYAKRLGVPIEVMVNSNRAARWDFGGRIV
                     ANRQAVNDVRGGIAELMVKAVRKGALDEELSAVDKSVMVNFLGAYGGLSRLNGEYRGD
                     GRSGYTTLPGAYGEAGTTVPPMKLEDILKQRGWVLPLLFEEIFDQQAPMFQPVGGMDR
                     IAHALYEQVKPAVRLRTPVTAIRRVGDRVRIEHGAARTDADYCICTLPVNLLARIPND
                     FSAAKQAAMKDIAYVPAVKAAFQSRRFWEDEGIYGGVGWTDALSENVLYPSGGWHGDK
                     GILVAAYVAGWTGQDHPEKFTALSHEERFRICREVIGRMHPGRAGELERPVTVAWGLT
                     RWSEGVGPGDPAWFESPRPERYAELYRPEGPIFFAGEHLSYVQFWQEGAALSSHAAMK
                     ALEARVREEALTRRAERRPAAA"
     gene            complement(6697..7191)
                     /locus_tag="VF547_09110"
     CDS             complement(6697..7191)
                     /locus_tag="VF547_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8263019.1"
                     /translation="MRLLATVAIGLLAASAGEAKDVRRLGAAESPILSSAEVPAGSRL
                     VWVSGTVPDPVDPAAPAGSVQRYGDTAAQTRSVLRKIEAQLRQHGMGLGDIVMMRVFL
                     VAPPGQARMDFAGMMTAYREFFGTAAQPNKPARSTLQALGLVDPGWLVEIEATAARPK
                     DKSK"
     gene            complement(7282..7572)
                     /locus_tag="VF547_09115"
     CDS             complement(7282..7572)
                     /locus_tag="VF547_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263020.1"
                     /translation="MARLQVEARPGAGVSGYSLFVDGIPVTMDVGHRGEARCDGRCGD
                     GSRHALLYSFSGSPGATLSIVLRCRDREVCRVAATRTAGRGVTGAGREPFDL"
     gene            7882..8109
                     /locus_tag="VF547_09120"
     CDS             7882..8109
                     /locus_tag="VF547_09120"
                     /inference="COORDINATES: protein motif:HMM:NF020472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1843 domain-containing protein"
                     /protein_id="HEX8263021.1"
                     /translation="MGIPPISKQALTLETLRKLLDPGRVFYRPAVDDALKRGDLAEIQ
                     ALIKGARDVKTKYGDLDGLIRQLEAAAKKAQ"
     gene            8111..9337
                     /locus_tag="VF547_09125"
     CDS             8111..9337
                     /locus_tag="VF547_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8263022.1"
                     /translation="MEAWRADSVPVRYAALDDIVRRVPVSVVWEITLACDLACSHCGS
                     RAGRRRRDELTTAEALDLVEQIAELGARDVGLIGGEAYLRKDWLDIVRAIRAAGMRCD
                     LQTGGRNLTEARVAAAAEAGLCGVGISIDGIGATHDRMRGVPGSYELALAALRRVRDH
                     GLSSAVNSQINAFTLPQLRELMDVIIDAGATNWQLAITTAMGNAADHPEMLLQPWQML
                     EVMPLLAELAVEGRERGLFLQPASNVGYYGPHESTIRNVLDPEIFFHGCNAGDTVMGI
                     EADGTIKGCPGLGSTYAGGNVREKRLKEIWETSEPIAFNRRRTVDDLWGFCRTCYYAE
                     TCMAGCTWTSHALMGRAGNNPMCHHRALEMDARGLRERLVKVKDAPGRSFDNGEFEIV
                     VEPVPGRVPDRIAING"
     gene            9330..9542
                     /locus_tag="VF547_09130"
     CDS             9330..9542
                     /locus_tag="VF547_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263023.1"
                     /translation="MAETGRIPVPLRLCRGCRRFVNIDGREDCVFCGVDLAAAEAEHE
                     ANVAEMWRAAEALRAAMAAAEPASRD"
BASE COUNT         1397 a         3462 c         3395 g         1348 t
ORIGIN      
        1 ggtacttcca gcggccgaag aacccctcca ccgcgcggcg cgcggtcccc caccccccga
       61 aaccgcgggg aggaatttgc aagccgctca tctgcacttc ctccaacccg ggcccttcac
      121 gaaccgtccc ggcttcgcgc ccgtatgctc gccgtcgcga agcacggcga cgccgttgac
      181 gaggacgtgg cgcatcccga cggcgtagcg gtgcgattcc tcgtaggtgg cgcggtcggc
      241 gatcgtcgcc ggatcgaaga cggcgatgtc ggcgtggaag ccggggcgga gccgcccgcg
      301 gtcggcgagg cgcaggttgt cggcgggcag cgaggtcagc cggcgcaccg cctcggcgag
      361 gctcagcgcc ttctcgtcgc gcacgtagcg gccgagaagg cgcgcgaaat tcccgtaggc
      421 gcggggatgg gtcgccttgt cggtgaaggg cggctcggcg gcgatcgccg ccgcgtcgga
      481 gccgaagctg acccagggct tggccagcac cttgcgcaga ttgtcctccg agatggagtg
      541 gaagatggtg ccgaccgcgc tgtcgtcctc gacgatgagg tcgaccgccg tctcctccgc
      601 gctcttgccg cgccgagccg cgatctccgg cagccgccgg ccggcatgga cgcgcagcgc
      661 cggattggcg aagccgaccg gctggaccgt gttccagtcg cgcgagccgc cctgcatctc
      721 ggcaacgacc cgggctcgga gctcgggctt tttcagccga tcgatcatgg ccctcaggcc
      781 gccctcgcgg acccatatcg gaagggtcga atcgaggccg gtgcccgagg cggtgtagag
      841 gtacatgtcg gcggtcaccg gcactccggc cgcgcgcgcc gcctcgatcc gctcgagcgc
      901 ggcgtccatc agcggccagt tggatcgacc gactgccttg aggtggtaga tctcggcccg
      961 ggcgccggag cgtcggctga tctcgaccag ctcgtcgatc gccgggagga ggccgctgct
     1021 ctcgcttcgc atgtggctga tgtacatgcc gccgcagcgg ccggcctcgg ccgccagggc
     1081 gaccagctcg tcggtctcgg catagattgc cggcacgtag atgagcgagg agccgacccc
     1141 catcgcgccc tcgtccatcg cccggcgcac cagcgcgcgc atccgcgcca gctgctcggg
     1201 ggtcggatcg acgtcgttgc ggccgagctc gtggacccgg atcgtcgagg cgccgaccat
     1261 cgaggcgacg ttgacgctga cccccttgcg ctcgaggaag gccaggaact cgcccagcgt
     1321 cgtccaggag atcggataac gggtggtggt ctgctcggcc agcgcctccg ccttctgcgc
     1381 ggggctgagc ggacccatcg accagccttc gcccatcact tcgagggtga cgccctggcg
     1441 gacgtcgctc tgcgcccggc cgtcgcggat caggggctcg gccgcccagc tgagcatgtt
     1501 gatgaagccc ggcgcgacgg cgaggccccg gacgtcgatc tcttgccgcg ccttgccggg
     1561 gatgcgcggg gcgatcgcgg cgatccggtc gcccctgatc gcgacgtcgc cgacaaaagg
     1621 cgcggcgccg ctgccgtcgt agaccgtgcc gccccgcagg atcgtgtcgt aggccggcgc
     1681 ggcctcgcgg gcggaggcgc agccggacag gataaggaga aaggcaacga gaacgcgggg
     1741 cattgcctca cgctagcggg actgagggta aagtctatcc ttggcagaga ggccctatcc
     1801 ttcaatccct cccccggcat ttgccgggga gggggaccag gcgaagcctg gtggaggggt
     1861 tcttcggact ctcgagaacc cgtccaccgc gctgcgcgcg gtccccctcc cccggaaagc
     1921 cgcgggagga atagagcggc tagtcgtgcc ggcccctgcg catctcggcg gcggcctcgc
     1981 gcatctcgcg ggcgccctcg cgcatgccct cggcgccttc ctccatgccc tcggcggcct
     2041 cgagcaggtc ctcgtgggtg aggcgcttgc cgcgtgcggc gtgctcgcgg atcttctgct
     2101 cccgataggc ggggtcgcga aggcggcgcg cctccgcctg catgtgggcg gcgccgcgct
     2161 ccatcccggc ggcgccattc tccatgccgc gggcgccgtt gatcatgccc gccttcgcat
     2221 tggcgagcgc gcgctcgacg gtgcggccga tctcggcgct ctgcgcctcg atcgcgcgcg
     2281 acgcctcggc caccgcggcc gcgacctccg cctcgatcgc cgcctcgtcg acctcgccgg
     2341 ccgaggcgtg ggccgcggcg cgggcatgag cgctggctcg gaccgccgcg gcgattgcgg
     2401 catgcgggac ctgagggggc gccgggacgc gcggcgggcg cacggcgatc acgggcagcg
     2461 gaggaaccgg cgtcggggcg gcgctcggcg ccgcgccggg ctgcggcggc acgaaggcgg
     2521 cggcgatcgg ggccggcgcg gccatcggcg ccatcggggc catcggcgcc acggccgagt
     2581 gcggcgacgg cggcgcggcg ggcgccgccg gcgcggccgg cgcctcgggg acgccttcga
     2641 cgaagtcgag cgccgcgacc ggggcggcga aggccgcgca accgagcatc gccgcgaccg
     2701 cccagaacga gccggagcgg cgcgagaccc ggccgtcgag gatcgccctg acgcgcttcg
     2761 aaagccccgg ctcgcccgcc gcgatggcat tggcggggag cgcgttggcg ccgctcagcc
     2821 gagcccagtc gagcagggtc tgggcgtagc gcgccggctc gaccgccgcc gcggcgtcgc
     2881 tgtccgcggc ctcctcggcc tgctgggcga cctcgcggtc gagcctccag accagcgggt
     2941 tgaaccagaa cagggcgacg gcgagcctcg acaggatcag cgagggccag tcgcgccggg
     3001 cgacgtgggc gacctcgtgg acgaggatcg cgtcggcctc ggccggagac cggagcgtgt
     3061 cgcggtcgag caggatcacc gggcggcgca ggccccagct gagcggcgaa accgcatcct
     3121 cggaggcgag caggcggatg ccgagcgagt cgccgccggc tcgggtcagc gccgagcgcc
     3181 attccggcgc ctcgatctcg cgcgccgccc gagtccagcg gcgcagggtc atcaggccgg
     3241 cgagaagccg cccgccgacc atcgccaccc cgcccgcgta gagcaggaag aagagcaggc
     3301 tgggatcgtc ccaattgtcc gaaggcccct ggagcgccgg ggactgggag gtcgccgagg
     3361 ccggggcggc cgcgagcggc tcgaacgcgg ccgcttcggc cggagcggca ggcgccgggg
     3421 ccgcggccgc cggcgcggcg gtctcgatct cgagcgccgg agcgaacagc gcgatggccg
     3481 gaagcgcgag cagcagcgcc accgcgacct tgagcacggc gccgcggtcg gccgcggcgc
     3541 gcgagcgaag cagcgccgcc agaagcagcg ccccgccggc gatcgccgcc gatttccagc
     3601 ccatctcgat caggaagtcg taggtcattg gtcgcgctcc tttgcctcgt cgatcgcgcg
     3661 ttgcagcgcg tcgagctcat cgggcttcag cgatttggaa agtccgagca gcgccgtcgc
     3721 cgcgctcgcc gccgacccgt cgaagaaggt cttgaccagc gccttgagcg cgctttcgcg
     3781 gaccttggcc ggctgcggca cgcttcggta gacgtaggtc tggtcgtcgg cgcggtgccg
     3841 gacgaggccc ttggcttcga gccgggcgag catcgtgcgc accgccgaat ggctcggcgg
     3901 atcggccatc gcccggcgca cgtcggcggc ggtcgcctcg ccggcgctgc acaagatctc
     3961 gaatatctcg cgctcgcggc gcggcaggct ggcgatcatc gactcggggt ccttgctgct
     4021 acacctgtag catgccacat ttgtagcggg ttgcaagtgc cattggtcga acggcggcga
     4081 agctccgtcg aaaacgggtc cgaagcgacc tagagcgatc cgcagtcaga atgcacctct
     4141 ctccgttcgg gctgagcttg tcgaagccgt ctccctttct ctcgtggaac gacaaggaaa
     4201 gcccttcgac aggctcaggg cgaacggaat gggagcgatc ggcccaatcc ctcgatgctc
     4261 caagccgttc ggctccgaca ggctcaagcc gatcggggag atgcccccgg gcgccaagct
     4321 cagcgcggct tccggaagcg gtagacgaag cggtccgtct tgccccggat ggacgggtcg
     4381 aagacgttct tcgaatggtc gtcctcggcc acccgcaaga tgtccgactg cgcctcgagc
     4441 acgaagcccg cccgctcgaa atcggcgcgg accaccgccg ggtcgatccg gtgcagcttc
     4501 tcgaccacct cgcgcgtgtc gcctccggcc gttgcgacat ggtcgatcac ggcgaccgtc
     4561 ccgcccggct tgacggcgtg ccagatcgcc tggacgaccg tgctggggtc gatccgcggc
     4621 agtccgaact gcgcgctctc ccaataggcg tcgtggtaga cgaggtggag cagggcgaag
     4681 tcgaagctgc tcggggcgaa ggcgatcgcc gtcgatgggc tcgagtagaa ggcggtattg
     4741 ggatttcggg cccgaatgtc ggcgagggcc ttcaccacgg gctcgcgccg ggcgaaggag
     4801 gccgagttcc agccgacgac gtggccccgc ggccccaccg ccttggcgat gatgtccgca
     4861 taatagccgg tgccggtgaa gtaatcgagg acgcggtcgc ccttcttcag gcccatgaag
     4921 cgaagcacct cggccggctt cctgaccgcg tcgaggtcgg tcgccgcctt cggccggccc
     4981 ggcgccgcga tcgcggcggc gacgtcggca ggcgcccgcg ccggagccat ggccgccgcg
     5041 ggcgcggccg tcgccagggc catgatcagc cagaaggttg cgcgcatctc ggtcctccct
     5101 ttcccattca cgccgccgcg gggcgccgct cggcgcgccg ggtcagcgcc tcttcccgga
     5161 ccctcgcctc gagagccttc atggcggcgt gcgacgaaag cgccgccccc tcctgccaga
     5221 actggacgta gctcagatgc tcgccggcga agaagatcgg cccctcgggc cggtacagct
     5281 cggcatagcg ttccgggcgc ggcgattcga accaggccgg gtcgcccggg ccgactccct
     5341 cggaccagcg ggtcaggccc caggcgaccg tcaccggccg ctcgagctcc ccggcccgcc
     5401 cggggtgcat gcgcccgatc acctcgcggc agattcggaa gcgctcctcg tggctgaggg
     5461 cggtgaactt ttcgggatga tcctgcccgg tccagcccgc cacgtaggcg gcgaccagga
     5521 tgcccttgtc gccgtgccag ccgcccgagg gatagagcac gttctccgac aaggcgtcgg
     5581 tccagccgac gccgccgtag atcccctcgt cctcccagaa gcgccgcgat tggaaggcgg
     5641 ccttgaccgc cggaacgtag gcgatgtcct tcatcgccgc ctgcttggcg gccgagaagt
     5701 cgttggggat ccgcgcgagc aggttcaccg gcaaggtgca gatgcaataa tcggcgtcgg
     5761 tcctggcggc gccgtgctcg atccggaccc ggtcgccgac ccgccggatc gcggtcaccg
     5821 gggtgcgcag ccgcaccgcc ggcttgacct gctcgtagag ggcgtgcgcg atccggtcca
     5881 tgccgccgac cggctggaac atcggcgcct gctggtcgaa tatctcctcg aacagcagcg
     5941 gcaggaccca gccgcgctgc ttcaatatgt cctccagctt catcggcggc accgtcgtcc
     6001 cggcctcgcc ataggcgccg gggagcgtcg tgtagccgga gcggccgtcg cccctatact
     6061 cgccgttaag gcgggacagg ccgccatagg cgccgaggaa attgaccatc acgctcttgt
     6121 cgacggcgct gagctcctcg tcgagcgcgc ccttgcggac cgccttgacc atcagctccg
     6181 cgataccgcc gcgaacgtcg ttgaccgcct ggcgattggc gacgattcgg ccgccgaaat
     6241 cccacctcgc ggcgcggttg gaattgacca tgacctcgat cggcacgccg aggcgcttcg
     6301 cgtagcccag gatcgtatga tgggcggtcg ggatgcgcgc cgcgccggcg ttgagataga
     6361 ggcccttgga aaagccgcag acctggtcgg gccgcccgat ctgctcgatc cggtcgccgc
     6421 cgcgaagggt ccagacccgc ccgccgatcc ggtcccgcgc ctcgagcacg gtgacgtgcc
     6481 agccggcgcg gcgaagctca taggcggcga ccaggcccgc tattccggcg ccgaggacga
     6541 ccaccgactt gcgcttcccc ggcggaggcg gaagctcgaa cggctcggcg gcggcgggcg
     6601 gggcgttgag cagccccatc gcctccatcc cgagatagac ggcgctgact ccgccggcga
     6661 ggccgagccg ctcgagcagg gctcgccggg tgaggctcac ttcgacttgt ccttcggcct
     6721 ggcggcggtc gcctcgatct cgaccagcca gccgggatcg accaggccca aggcctggag
     6781 agtcgagcgc gcgggcttgt tcggctgcgc ggccgtgccg aagaactcgc gataggcggt
     6841 catcatcccg gcgaaatcca tccgcgcctg gcccggcgga gcgaccagga agacgcgcat
     6901 catgacgatg tcgccgagcc ccatgccgtg ctgcctcagc tgcgcctcga tcttcctcag
     6961 caccgagcgg gtctgcgcgg cggtgtcgcc gtagcgctgg acggagccgg ccggcgcggc
     7021 cggatcgacc gggtccggca cggtcccgct cacccagacg agccggctcc cggccggcac
     7081 ctcggccgac gacaggatcg gcgattcggc cgcgccgagc cgccgaacgt ccttcgcctc
     7141 ccccgccgaa gcggcgagca gcccgatcgc cacggtcgcc agcaagcgca tcgcagtccc
     7201 cctttgcaga ccgatgcgag cgtagcgcag ccgccgggcg tcgtcacccc ggccggaacc
     7261 ggcttcgtcg ctgcagaacg gtcataggtc gaacggctcc cggcccgcgc ccgtcactcc
     7321 gcgccccgcg gtccgggtcg ccgcgactcg gcagacctcg cggtcccggc agcgcaggac
     7381 gatgctcaga gtcgcgcccg gcgacccgct gaagctgtag agcagcgcgt ggcgcgagcc
     7441 gtcgccgcag cggccgtcgc agcgcgcctc gccgcgatgg cccacgtcca tcgtcaccgg
     7501 aatgccgtcg acgaacagcg aatatccgga cacgccggcg cccggccgcg cctctacctg
     7561 cagcctcgcc atctatgcct ccacgggatc gcgacggctc ctaggcccga tcgggaagga
     7621 cgtctgtgac ctccctcacg caacgccgaa gcacccgcaa accggcgcag ttatatccga
     7681 tttggatgaa aatgaccggc tttgggcgcc cgcgccagct tactgattcg ccgggccccg
     7741 cccctccggc ctagggggga agcgtccgcc ggccgccccg cgcaccggcc ggattcatcc
     7801 atttcagagc aaactcgcgc ttccgccgcc gtgcccgcca cctagtcttg ccgggcgaat
     7861 caaccgccgg gagatgacag catgggcatt ccaccgatca gcaagcaggc gctcaccctc
     7921 gagacccttc gcaagctgct ggatccgggc cgggtcttct accgccccgc cgtcgacgat
     7981 gccctgaagc gcggcgacct cgccgagatc caggcgctga tcaagggcgc gcgcgacgtc
     8041 aagacgaaat atggcgacct cgacggcctg atccgccaac tcgaagcggc cgccaagaag
     8101 gcgcagtaag atggaggcat ggcgggcgga ctccgtgccc gtccgttatg cggcgctgga
     8161 cgacatcgtc cggcgcgtcc cggtgagcgt ggtctgggag atcacgctcg cctgcgacct
     8221 cgcctgcagc cattgcggct cgcgcgccgg gcgaaggcgc cgcgacgagc tcaccaccgc
     8281 cgaagcgctc gacctggtcg agcagatcgc cgagctcggc gcgcgcgacg tcggcctgat
     8341 cggcggcgag gcgtatctca ggaaggactg gctcgacatc gtccgggcga tccgggcggc
     8401 ggggatgcgc tgcgacctcc agaccggcgg ccgcaacctc accgaggcgc gagtcgcggc
     8461 ggcggcggag gccggcctgt gcggagtcgg catctcgatc gacggcatcg gcgcgaccca
     8521 cgacaggatg cgcggagtcc cgggctccta cgagctcgcg ctcgcggcgc tgcggcgggt
     8581 ccgcgaccac ggcctttcct cggccgtcaa cagccagatc aacgccttca ccttgccgca
     8641 gctgcgcgag ctgatggacg tcatcatcga cgccggcgcg accaactggc agctcgccat
     8701 caccaccgcc atgggcaatg ccgccgacca tcccgagatg cttctccagc cctggcagat
     8761 gctggaggtc atgccgctgc tcgccgagct tgcggtcgaa gggcgcgagc gcggcctgtt
     8821 ccttcagccg gccagcaacg tcggctatta cggcccgcac gaatcgacca tccgcaacgt
     8881 cctcgacccg gagattttct tccacggctg caacgccggc gacacggtga tggggatcga
     8941 ggcggacggc acgatcaagg gctgccccgg cctgggctcc acctatgccg gcggcaacgt
     9001 ccgggagaag cggctcaagg agatctggga gacgtccgag ccgatcgcct tcaaccgccg
     9061 ccggacggtc gacgatctct ggggcttctg ccgcacctgc tattatgccg agacctgcat
     9121 ggccggctgc acctggacca gccacgccct gatgggccgg gccggcaaca acccgatgtg
     9181 ccaccaccgc gcgctcgaga tggacgcccg cggcctgcgc gagcggctgg tcaaggtgaa
     9241 ggacgcgccg ggccgctcgt tcgacaatgg cgagttcgag atcgtcgtcg agccggtgcc
     9301 ggggcgagtg ccggaccgga tcgcgatcaa tggctgagac cggccggatc cccgtcccgc
     9361 tgcgcctgtg ccgcggctgc cggcgtttcg tgaacatcga cgggcgcgag gactgcgtgt
     9421 tctgcggcgt cgacctggcg gcggccgaag ccgagcacga ggcgaacgtc gccgagatgt
     9481 ggcgcgcggc ggaggcgctt cgggcggcga tggcggcggc cgagcccgca tcgagggatt
     9541 aggtttaatc accctcgccc gttcgccctg agcctgtcga agggccctcc ttctcgttcc
     9601 at
//
LOCUS       DASVQZ010000327         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_455757, whole genome shotgun sequence.
ACCESSION   DASVQZ010000327 DASVQZ010000000
VERSION     DASVQZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455757"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VF547_09135"
     CDS             complement(<1..1485)
                     /locus_tag="VF547_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEX8263024.1"
                     /translation="MRALLAVSAGLAATSGDAAPVLHPLFSNHAVLQRGGPIAVWGTA
                     EPGERVTVTLGGASRTAAAGADRAWRVELPAMAAGGPYRLEAAGRGGARATAEDILIG
                     DVWLCSGQSNMEFPVARALNGDSEAAGAGDNRLRLLTVPRKTATSAAAGFGGPVAWAP
                     SSPESVRDFSAACYFMARDLRGSQKVPIGAIAASWGGTAIRSWMDEASGRAVGGEDSA
                     LLALYRRDPAAANRRFGERWQAWWRARSPLEPWRDASALAWKPMPRIGYWEGWGDPAF
                     EAFNGMMWARKTVTLSREEAARPATLSLGVVDEIEQTWVNGVPVGNTFGWDVARDYPL
                     PAGLLREGENVILVNLYDAYGAGGFQGPAEALKLGFAGGGAKALGEGWEYAVAPSGLG
                     EPPRPPWDTAAGLSLIHNGMIAPLGRFGLRGVAWYQGETDGGMPSGYAGKLRAMMAGW
                     RRQFGAPDLPFLVVSLANYGAPAWAPRASGWAEVREAQRQVTMAD"
     gene            complement(1490..2248)
                     /locus_tag="VF547_09140"
     CDS             complement(1490..2248)
                     /locus_tag="VF547_09140"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8263025.1"
                     /translation="MSEASDNRAVYPSLRGKRVLVTGGGSGIGAGIVEAFVRQGADVT
                     FFDIAEEDSSALAGRLGARFERLDLRDIAGLQAAIASLVESGGGFDVLVNNAANDDRH
                     RVDEVTEAYWDERVDVNLKHFFFCAQAVVPGMRAKGGGAIVNLGSISWHLALPDLPIY
                     QTAKAAIEGLTRSLARDLGKDGIRVTCVVPGNVRTPRQLQWYTPEGEAEIVAGQCLGG
                     RLAPEDVAAMVLFLASDDARLVTGHEYFVDAGWR"
     gene            complement(2255..3691)
                     /locus_tag="VF547_09145"
     CDS             complement(2255..3691)
                     /locus_tag="VF547_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8263026.1"
                     /translation="MADTLTHFIGGEAVAGEAAGESLNPSNLDDVVARYPKATPADVD
                     SAVKAAREAFPGWSEGSPEVRSDVLDKAGSMIMARAPELGRLLSREEGKTLAEGTGEV
                     MRAARIFKYFAGEALRRHGQALDSTRPGIDVTTHREAVGVFGLITPWNFPIAIPAWKT
                     APALASGNTVVLKPATPTPAIAHALASIIQEAGAPPGVFNMVIGEGAAGQAMVDHEGV
                     DGISFTGSQYVGGMVAQGAIKRQARVQLEMGGKNPLVVLDDADLERAVTCAIDGAFFG
                     TGQRCTASSRIIVTENIHDRFVEALAERARGLRVGDALDPSSQIGPAVSESQLEQNLR
                     YVGIARDEGGRVLTGGEILTLATRGYFMAPTVIADTAAEMRINCEEVFGPVASTVRVR
                     DYEEALEIANRGQFGLSAGIVTNSLKHARHFRRAVRAGMVMINLPTAGVDYHVPFGGT
                     RKSSYGAREQGFAAIEFYTQTKTVYIGG"
     gene            complement(3684..4838)
                     /locus_tag="VF547_09150"
     CDS             complement(3684..4838)
                     /locus_tag="VF547_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8263027.1"
                     /translation="MLPADLPRAVLAGRLLLDAGPTPVLVRDGIVEDVSAAAPTISDL
                     LDLDELRSVPGRRLFPVEELAERIAAAGERPFLLAPVDLQVVKAAGVTFAVSAIERVI
                     EERARGDADAALGIREKLEARIGGAIRSVVPGSPEAARLKDALIEDGLWSQYLEVAIG
                     PDAEIFTKTALLASVGWGADIGVRSDSSWNNPEPEVVLAVDSRGRAVGATLGNDVNLR
                     DFEGRSALLLSKAKDNNASCAIGPFIRLFDEHYTLDDVRQARVELVVEGADGFRLEGV
                     SSMAEISRDPEELVRQALSEHHYPDGFVLFLGTLFAPVQDRDEPGRGFTHKEGDRVTI
                     STPGLGALVNRVTTSRAAPPWTFGVRALMRNLAGRGLLSGGHSEGATPGG"
     gene            complement(4976..>6116)
                     /locus_tag="VF547_09155"
     CDS             complement(4976..>6116)
                     /locus_tag="VF547_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX8263028.1"
                     /translation="VAGDGADNIAKQSGGWTITWQGPGLTNADFPGATSIFAGIRDAV
                     RGAGGRATLSPDGSFSRKPDVAIVVFGEDPYAEFVGDRPRTLEFSPADKKHLDLLRKL
                     KAADVPVVAVFLSGRPMWVNPELNASDAFVAAFLPGSEGAGIADVLFRDASGRVRHDF
                     KGRLSYSWPRRADQTPLNRGDAAYDPLFAYGHGLTYRDKAVIPRLPEERPAGSEFPDG
                     VFFARGALPPGWTFAVGGEARTRRVDRRAQEDAIAATFTGTGPQTLRLLPREALDISR
                     EATGELSLLVDYRVDSAPTGRVTLSMACGEGCAGTVPVKGLLAGPSGEWRTMTVPLRC
                     FVQAGLDPGRVQSPFTLATSGRLELAVSDIRIASATVDQQKCAWD"
BASE COUNT          896 a         2165 c         2192 g          863 t
ORIGIN      
        1 gtccgccatc gtcacctgac gctgggcctc gcggacctcg gcccagccgc tggcgcgcgg
       61 agcccaggcc ggcgcgccgt aattggcgag gctcacgacg aggaagggca ggtccggcgc
      121 tccgaactgc cggcgccagc ccgccatcat cgcccgcagc ttcccggcat agcccgacgg
      181 catgccgccg tccgtctcgc cctggtacca ggcgacgccc ctcaggccga accgcccgag
      241 cggcgcgatc atgccgttgt ggatgagcga aaggccggcg gcggtatccc agggcggacg
      301 gggcggctcg cccaggccgg agggggcgac ggcatattcc cagccttcgc ccagcgcctt
      361 ggcgccgccg ccggcgaagc cgagcttgag cgcctccgcg ggcccctgga agccgcccgc
      421 cccataggcg tcgtaaagat tgacgaggat gacgttctcc ccctcgcgca acaggccggc
      481 gggcaatgga taatcccgcg ccacgtccca gccgaacgtg ttgccgacgg gcacgccgtt
      541 cacccaggtc tgctcgatct cgtcgaccac gccgagcgaa agcgtcgccg gccgagccgc
      601 ctcctcccgg ctcagggtga cggtcttgcg cgcccacatc atgccgttga aggcctcgaa
      661 ggcggggtcc ccccaccctt cccaatagcc gatcctcggc atcggcttcc aggcgagcgc
      721 cgaagcgtcc cgccagggct cgagcgggga ccgggcccgc caccaggctt gccagcgctc
      781 gccgaagcgc cggttcgcgg cggcggggtc gcgccggtag agggcgagca gcgccgagtc
      841 ctcgccgccg acggcgcggc cggacgcctc gtccatccag gatcggatcg cggttccgcc
      901 ccagctggcg gcgatcgctc cgatcggcac cttctgcgag ccgcgaaggt cgcgcgccat
      961 gaagtagcag gccgccgaga agtcgcggac ggactcgggg gaggagggcg cccaggcgac
     1021 cgggccgccg aaacccgcgg cggcgctggt ggccgttttc ctcggaacgg tgaggagccg
     1081 aagccgattg tcgccggcgc ccgccgcctc cgaatcgccg ttgagggcgc gcgccacggg
     1141 aaattccatg ttggactggc ccgagcagag ccagacgtcg ccgatcaata tgtcctccgc
     1201 cgtcgcccgg gccccgccgc gcccggccgc ttccaggcga taggggcccc cggcggccat
     1261 ggccggcagc tcgacgcgcc aggcgcgatc ggcgccggcg gccgcggtgc gcgaggcgcc
     1321 gccgagcgtg acggtgaccc gctcgccggg ctcggccgtg ccccacacgg cgatcgggcc
     1381 gccgcgctgg aggacggcat gattggaaaa gagcggatgg aggaccggcg ccgcgtcgcc
     1441 cgaggtggcg gccagcccgg ccgaaacggc gagcagcgcc ctcaacgcct catctccagc
     1501 cggcgtccac gaaatattcg tggccggtga ccagccgcgc gtcgtccgag gcgaggaaga
     1561 ggaccatcgc cgccacgtcc tccggcgcca gccgcccgcc gaggcactgc ccggcgacga
     1621 tctccgcctc gccctcgggc gtataccatt gcagctgacg cggcgtccgg acattgccgg
     1681 gaacgacgca ggtgacgcgg atgccgtcct tgccgaggtc gcgggcgagc gagcgggtga
     1741 ggccctcgat ggcggccttc gcggtctggt agatcggcag gtcgggaagc gcgaggtgcc
     1801 aggagatgga gccgagattg acgatcgccc cgccgccctt cgcccgcatc cccggcacca
     1861 ccgcctgggc gcagaagaag aagtgcttca ggttcacgtc gacgcgctcg tcccaatagg
     1921 cctcggtgac ctcgtcgacc cggtgccggt cgtcgttggc ggcattgttg acgaggacgt
     1981 cgaacccgcc tccggactcc acgagactcg cgatggcggc ctgcaggccc gctatgtccc
     2041 gcaggtcgag ccgctcgaag cgggcgccca gacggcctgc gagcgcgctc gaatcctcct
     2101 cggcgatgtc gaagaaggtg acgtcggcgc cctggcgcac gaacgcctcg acgatcccgg
     2161 cgccgatgcc cgagcccccg ccggtgacga ggacgcgctt tcccctcagg ctgggataga
     2221 cggcgcggtt gtcgcttgct tcactcacta ggtctcagcc cccgatgtag accgtcttgg
     2281 tctgcgtata gaattcgatc gcggcgaagc cctgctcgcg cgcgccgtag ctcgatttgc
     2341 gggttccgcc gaacggcacg tgatagtcga cgccggccgt cggcaggttg atcatcacca
     2401 tgccggcgcg gacggcgcgg cggaaatgac gcgcatgctt gagggagttg gtgacgatcc
     2461 ccgccgaaag gccgaactgg ccgcgattgg cgatctccag cgcctcctca tagtcgcgga
     2521 ccctgaccgt gctcgcgacc ggtccgaaca cctcctcgca attgatgcgc atctcggcgg
     2581 ccgtatcggc gatgacggtc ggcgccatga agtagccgcg cgtcgccagg gtgaggatct
     2641 cgccgccggt gaggacccgg ccgccctcgt cccgggcgat tccgacatag cgcaggttct
     2701 gctcgagctg gctctcgctg acggcggggc cgatctggct ggacggatcg agcgcatcgc
     2761 cgacccgcag gccgcgcgcg cgctcggcca gcgcctcgac gaaccggtcg tggatgttct
     2821 cggtgacgat gatgcgcgag gaggcggtgc agcgctggcc ggtgccgaag aaggcgccgt
     2881 cgatcgcgca ggtgacggcc cgttcgaggt cggcgtcgtc gagcacgacc agcgggttct
     2941 tgccgcccat ctcgagctgc acccgggcct gacgcttgat cgccccttgg gcgaccattc
     3001 cgccgacata ttgggaaccg gtgaaggaga tgccgtcgac gccctcatgg tcgaccatcg
     3061 cctggccggc cgcgccttcc ccgatgacca tgttgaagac gccgggaggc gcccccgctt
     3121 cctggatgat cgaggccagg gcgtgggcga tggcgggcgt cggggtcgcc ggcttcagga
     3181 ccaccgtatt gccggaggcg agcgccggcg ccgtcttcca cgccgggatc gcgatcggga
     3241 agttccaggg cgtgatcagg ccgaacacgc cgacggcttc ccggtgggtc gtcacgtcga
     3301 ttccggggcg ggtcgaatcg agcgcctggc cgtggcgccg gagcgcctcg ccggcgaaat
     3361 atttgaagat gcgcgccgcg cgcatgacct cgcccgtgcc ttccgcgagg gtcttgccct
     3421 cctcgcggct gagcagcctg cccagctctg gggcgcgggc catgatcatc gagcccgcct
     3481 tgtcgagcac gtcggaacgc acctccggcg agccttcgga ccagccggga aaggcttcgc
     3541 gggccgcctt gacggcgctg tcgacgtcgg ccggagtcgc cttggggtag cgcgcgacga
     3601 catcgtcgag gttggaggga ttgaggcttt cgccggcggc ctcgccggcg acggcctcgc
     3661 cgccgatgaa atgcgtgagg gtgtcagcca ccgggtgtcg ctccttccga atgccccccg
     3721 ctcaacagcc cccgtccggc gaggttgcgc atcagcgcgc gcacgccgaa tgtccagggc
     3781 ggcgccgccc gggacgtggt cacgcgattg accagggccc ccagcccggg ggtcgagata
     3841 gtgacccggt cgccctcctt gtgggtgaag ccccggccgg gctcgtcacg gtcctggacg
     3901 ggcgcgaaga gggtgccgag gaagaggacg aagccgtcgg gataatggtg ctcgctcagc
     3961 gcctgccgga cgagttcctc cggatcgcgg ctgatctccg ccatcgagct gacgccctcg
     4021 agccggaagc cgtcggcgcc ctcgacgacc agctcgaccc gcgcctgccg gacgtcgtcc
     4081 agggtgtaat gctcgtcgaa gaggcggatg aacgggccga tggcgcagga ggcgttgttg
     4141 tccttcgcct tgctcaggag aagcgcgctt cgtccctcga agtcgcgcag gttgacgtcg
     4201 ttgccgagag tggctccgac ggcccggccg cggctgtcga cggccagcac cacctccggc
     4261 tcggggttgt tccagctcga atcggagcgg acgccgatgt cggcgcccca gccgacgctg
     4321 gcgagcaagg ccgtcttggt gaagatctcc gcgtccgggc cgatcgcgac ttccagatat
     4381 tgcgaccaaa gcccgtcctc gatcagcgca tccttcaggc gcgccgcttc cggcgagccg
     4441 ggcacgacgg accggatcgc gccgccgatc cgcgcctcaa gcttctcgcg gatgcccagg
     4501 gcggcgtcgg cgtcgccgcg cgcccgctcc tcgatcaccc gctcgatcgc ggagacggcg
     4561 aaggtcaccc cggccgcctt gacgacctgc aggtcgaccg gcgcgagcag gaagggccgc
     4621 tccccggccg ccgcgatcct ctcggcgagt tcctcgaccg gaaacaggcg ccggcccggg
     4681 accgaacgca attcgtccag gtcgaggaga tcggagatcg tcggcgccgc cgccgacacg
     4741 tcctcgacga ttccgtcgcg gaccaggacg ggggtcgggc cggcgtcgag cagcaggcgg
     4801 ccggccaaaa ccgcccgcgg caggtcggcc ggcaacatcg cggttccaaa cgccgggctg
     4861 atcgctacca tccccaatcc tcgtttgtgg tagcgctacc aatcgagaag ggcggcgtac
     4921 agggccaatt accagaaaac ggaatttgtg cgggcgtcgg ggctcgcgga ccggctcagt
     4981 cccaggcgca tttctgctgg tcgacggtcg cgctggcgat tcggatgtcc gagacggcga
     5041 gctcgagacg cccggaggtg gcgagcgtga acgggctctg cacccggccg ggatcgagcc
     5101 ccgcctggac gaagcagcgc agcggcacgg tcatggtgcg ccattcgccc gagggtccgg
     5161 cgagcaggcc cttgacgggc accgttcccg cgcaaccttc cccgcaagcc atggacaggg
     5221 tcacccggcc ggtcggcgcc gaatcgacgc ggtagtcgac gagcaggctg agctcgcccg
     5281 tcgcctcgcg cgagatgtcc agggcctccc tgggcaggag cctcagcgtc tgcgggcccg
     5341 tgcccgtgaa ggtggcggcg atcgcatcct cctgcgcgcg gcggtcgact cggcgggtgc
     5401 gcgcctcgcc gcctaccgcg aaggtccagc cgggcggcag ggccccgcgg gcgaagaaga
     5461 cgccgtcggg gaattccgag ccggcgggcc gctcctccgg cagtcgcggg atcaccgcct
     5521 tgtcccggta ggtgaggcca tggccgtagg cgaagagggg gtcgtaggcg gcgtcgccgc
     5581 ggttgagcgg ggtctggtcg gcgcgccgcg gccaggaata ggaaagccgg cccttgaagt
     5641 cgtggcggac gcggccggag gcatcgcgga acaggacgtc ggctatgcct gcgccctcgc
     5701 tccccggcag gaaggcggcg acgaaggcgt cggaggcgtt cagctcggga ttgacccaca
     5761 tcggccggcc ggacaggaac accgcgacca ccgggacgtc cgccgccttc agcttccgaa
     5821 gcaggtcgag atgcttcttg tcggcagggc tgaattcgag cgtccgcggc cggtcgccga
     5881 cgaattccgc ataggggtcc tcgccgaaca cgacgatggc gacgtccggc ttccgggaga
     5941 agctgccgtc ggggctgagc gtggcgcggc ctccggcccc cctcaccgcg tcccggatgc
     6001 cggcgaagat ggaggtggcg cccgggaagt cggcgttggt cagcccgggt ccctgccagg
     6061 tgatggtcca gccgcccgat tgcttggcga tattgtcggc gccgtcgccg gcgacg
//
LOCUS       DASVQZ010000328         8782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_549376, whole genome shotgun sequence.
ACCESSION   DASVQZ010000328 DASVQZ010000000
VERSION     DASVQZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549376"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VF547_09160"
     CDS             complement(<1..296)
                     /locus_tag="VF547_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8263029.1"
                     /translation="MKGYCDDIEKATLANGDFRRVLYTGENLQLVLMTLPPGCDIGEE
                     VHEDRDQFFRIEEGRGTIYIDGAPNRVEDDFAVIVPAGARHNVVNDGDAPLRLYT"
     gene            complement(293..628)
                     /locus_tag="VF547_09165"
     CDS             complement(293..628)
                     /locus_tag="VF547_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3140 domain-containing protein"
                     /protein_id="HEX8263030.1"
                     /translation="MKLDRAAVAAAFAAAVNMTADEIEAWLETPESLAVGQKKDGGES
                     IGHASGRRIAAILRTPEPERTPDDYVHMRKVVGFVRRHRAQEPANPVTSRWRRSLMNW
                     GHDPLRSMT"
     gene            complement(684..1964)
                     /locus_tag="VF547_09170"
     CDS             complement(684..1964)
                     /locus_tag="VF547_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8263031.1"
                     /translation="MRPSLPAALLLACAAAPPARAASVVVTADRMIDVLAGRTVEEPV
                     IVITDGRIASVVGRGGARPVIPAGATRIDLPGKTLLPGLIDMHVHLDSNPRYGGYTSL
                     QFTDSFWVAQGVRNARDMLEAGFTTVRNVGSENYSDVGYKQAVDEGLMAGPRIVPAAH
                     ALSATGGHCDQTYLPPSLNAKGKAVGDGPQELRNRVREQRKYGAEVIKVCATGGVFSR
                     NNEPGQQQLSEEELRAIAEEAHQWGLKVAAHAHGAAGIKAAIRAGIDTIEHASLIDPE
                     GIRLAKERGTWLSMDIYNTEYTQAEGAKNGVLEDNLRKDREIAQLQRDNFRAAHRAGV
                     RMVFGTDAGVMPHGSAGGQFRVMVQYGMTPMEAIQSATRNAAQALGRERDVGAIAVGR
                     WGDIIAVDGDPLADVRQLEDVDVVIKGGELVKRD"
     gene            complement(2133..2672)
                     /locus_tag="VF547_09175"
                     /pseudo
     CDS             complement(2133..2672)
                     /locus_tag="VF547_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
     gene            complement(2962..3168)
                     /locus_tag="VF547_09180"
     CDS             complement(2962..3168)
                     /locus_tag="VF547_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263032.1"
                     /translation="MIELDDPSGVKRASQANRKARFNACALSRAKLAGRFPELDAELA
                     GLSYGRRYQGPGRSPDRADAMVWA"
     gene            complement(3730..5100)
                     /gene="lpdA"
                     /locus_tag="VF547_09185"
     CDS             complement(3730..5100)
                     /gene="lpdA"
                     /locus_tag="VF547_09185"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8263033.1"
                     /translation="MADSYDLIVLGSGPGGYVAAIRASQLGMKVAIVEREKLGGICLN
                     WGCIPTKALLRTSEIYHYMTHASAYGLVAEKPGFDLARIVDRSRKVAGQLNAGVKGLM
                     KKNKVTVVEGEGALKGKGALKVGDKALQAKHIIVATGARARDLPFAKADGERIWTYRH
                     AMVPKVMPTRLLVIGSGAIGVEFASFYSDMGAEVTIIEMLDRILPVEDEDVSAFVHKA
                     LVKQGMKIHVSSGVEKLESGPDGVKATLKGGDVREFSHAIVAIGIVPNTEGIGLEALG
                     VKTERGHIVVDGYGRTNVEGIYAIGDVTGPPWLAHKASHEGVVCVEAIAGGKPHPFET
                     WNIPGCTYSRPQVASVGLTEAKAKEQGRKVRVGKFPFIGNGKAIALGEAEGFVKTVFD
                     AETGELLGAHMVGAEVTELIQGYAVARQLETTEADLMHTVFPHPTLSEMMHESVLDAY
                     GRAVHY"
     gene            complement(5093..5512)
                     /locus_tag="VF547_09190"
     CDS             complement(5093..5512)
                     /locus_tag="VF547_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263034.1"
                     /translation="MRRAAAYLLTSLPVAAGVGGFLTWQIGRSPDPSAGLVREPLFVA
                     AILLAAWLATLAAGALLGLPLWLALRRLGLDRRPAVLLPAGLLAGSLAALVIGASLGG
                     FAAAAGDRTLLATGAAAGLASAALWPLLADHPRKDNG"
     gene            complement(5543..5752)
                     /locus_tag="VF547_09195"
     CDS             complement(5543..5752)
                     /locus_tag="VF547_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263035.1"
                     /translation="MALGKAVLGGLVASLAIPYVFRNDMGQLGEWSRLGLVRFTLGDT
                     PLGWSLPLFAAVTLFAWLFLSWAER"
     gene            complement(5754..7082)
                     /locus_tag="VF547_09200"
     CDS             complement(5754..7082)
                     /locus_tag="VF547_09200"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX8263036.1"
                     /translation="MPIELKMPALSPTMEEGTLAKWLVKEGDTVAAGDLLAEIETDKA
                     TMEFEAVDEGTIARILVAEGTDEVKVGTVIALIAGEGEDAAAAAPAAEAAKEPVGKDG
                     GETGAGQPAREVAAPAPAAASPKPPNPVQHSELAPQAGAEAGGRIKASPLARRLAEQR
                     GIDLAGLAGSGPGGRIVKADVDGAAGKAAGAPAPAQASVQAPAEAAPSYGVPDIPHEV
                     AKLSNMRKTIARRLTESKQNVPHIYLTVDIRLDALLKLRAELNASLEPRGVKLSVNDL
                     LIKALAASLIEVPECNVQYTGTELITFKRADISVAVSIPNGLITPVIVEADTKPLSRI
                     ATEMKDLAARAREGKLKPEEYQGGTASLSNMGMYGISVFSAVINPPQGMIMAIGAGEK
                     KPWVVDGALGVATMMTATGSFDHRAIDGADGARLMQAFKRLVEAPLAMLA"
     gene            complement(7186..8178)
                     /locus_tag="VF547_09205"
     CDS             complement(7186..8178)
                     /locus_tag="VF547_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8263037.1"
                     /translation="MKILLPLAAAAAFVLSACAAPSSAVGPLLSAGELRSLPVAEGEA
                     LTPDGVRLHYRVAGTGDAVVIAPFALYHGRSLDPLARGRRIVTYDPRGRGRSAAVPPA
                     KVSLDALLVDLDTVRRAVGAERVAIIGWSGAGMETFVYAMRHPGRVTRLVQLAPVAPR
                     FVPYGAQMMADRRTRTDAAAMATYEDRVRSGAFAGKPEEQCRESNAVSVPPLLADSAR
                     AALIPDVCASPNEHPDALAAYFGALFSSIDGYDWRGSLASVTIPRLIIHGERDNIPLA
                     GSEEWVMGQPNARLRVIAGAGHFPAYEQPEATLEAIAAFLGGDWPQGAEALPAR"
     gene            8357..>8782
                     /locus_tag="VF547_09210"
     CDS             8357..>8782
                     /locus_tag="VF547_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8263038.1"
                     /translation="MRSYLVVIDETSEARAALRYAARRASGTGGGVILLAVIPPAEFV
                     QWGGVQAAMEEEAKLRAEAMVLQASGAIVEEAGIEPTILVRQGEPEKEIADLLAERDD
                     VAALVLGAAEEGGPGPLVSHFSGAVAGSLPCPLVIVPGRL"
BASE COUNT         1251 a         3081 c         3084 g         1366 t
ORIGIN      
        1 gtgtagagcc ggagcggcgc gtcgccgtcg ttgacgacgt tgtggcgggc cccggccggg
       61 acgatcaccg cgaagtcgtc ctccacccgg ttgggcgcgc cgtcgatata gatcgtgccc
      121 cgcccttcct cgatccggaa gaactggtcg cggtcctcgt gcacctcctc gccgatgtcg
      181 cagcccggcg gcagcgtcat cagcaccagc tggaggttct cgccggtgta gagcacccgc
      241 cggaaatccc cgttggccag ggtcgccttc tcgatatcgt cgcaatagcc cttcacgtca
      301 tgctcctcaa agggtcgtgg ccccagttca tcagcgaccg gcgccagcgc gaggtgacgg
      361 ggttggccgg ttcctgcgcg cggtgacggc ggacgaagcc gacgaccttg cgcatatgga
      421 cgtaatcgtc cggcgttcgc tcgggttccg gagtgcgcag gatcgcggcg atgcgccggc
      481 ccgaggcatg gccaatcgac tcgccgccgt ccttcttctg tccgaccgcg aggctttcgg
      541 gcgtctcgag ccacgcctcg atctcgtcgg cggtcatgtt gacggcggcg gcgaaggcgg
      601 cggccacggc ggcgcggtcg agcttcatcc gaagcgccgg ggatcgatcc ggccgcgccg
      661 ccgcggaccg cgcgatcgag gcactagtcc cgcttcacca gctcgccgcc cttgatgacg
      721 acgtcgacgt cctcgagctg gcggacgtcg gcgagcgggt caccgtcgac ggcgatgatg
      781 tcgccccagc ggccgaccgc gatcgcgccg acgtcgcgct cccggcccag cgcctgcgcg
      841 gcgttgcgcg tcgccgactg gatcgcctcc atcggggtca ttccatattg caccatgacc
      901 cggaactggc cgccggcgct gccgtggggc atgacccccg cgtcggtgcc gaagaccatc
      961 ctcacgccgg cccggtgggc ggcccggaaa ttgtcgcgct ggagctgggc aatctcgcgg
     1021 tccttcctga gattgtcctc gagcacgccg ttcttggccc cttcggcctg ggtatattcg
     1081 gtgttgtaga tgtccatcga cagccaggtg ccgcgctcct tcgccagcct tatcccttcc
     1141 gggtcgatca gcgaggcgtg ctcgatcgtg tcgatcccgg cccggatcgc cgccttgatc
     1201 ccggccgcgc catgggcgtg ggcggcgacc ttcaggcccc attggtgggc ctcctcggcg
     1261 atcgctcgca actcctcctc ggacagctgc tgctggccgg gctcgttgtt gcgcgagaag
     1321 acgcccccgg tggcgcacac cttgatcacc tcggctccgt atttgcgctg ctcgcgcacc
     1381 cggttgcgga gctcctgggg gccgtcgccg accgccttgc ccttggcgtt gagcgaaggc
     1441 ggcagatagg tctggtcgca atggccgccg gtggcggaca gggcatgggc ggcggggacg
     1501 atccggggcc cggccatcag cccctcgtcg accgcctgct tgtagccgac gtcgctgtag
     1561 ttctcggagc cgacgttgcg gacggtggtg aagccggcct ccagcatgtc gcgcgcgttg
     1621 cgcacgccct gcgccaccca gaagctgtcg gtgaactgga ggctcgtgta gccgccgtag
     1681 cgcgggttgg aatcgagatg gacgtgcatg tcgatcaggc cggggaggag ggtcttgccc
     1741 ggcaggtcga tcctcgtcgc cccggccggg atgaccggtc gagcgccgcc ccggccgacc
     1801 accgaggcga tccggccgtc ggtgatgacg atgaccggct cctcgacggt ccgcccggcg
     1861 agcacgtcga tcatccggtc ggcggtgacg acgacgctcg cggcccgggc gggcggcgcg
     1921 gcggcacagg cgagcagcag ggcggcggga agggacgggc gcatcggcgg actccagggc
     1981 ttcggacaat cgcctcccta gccgcgcggc gtgggggccg tcactagccg cgtcgctcct
     2041 cgtccggctc agtccgtccc ggccggcacc tcctcgagcg gcccgcccat gccggtgaag
     2101 gcgccgtcgc cgcggaccat ggcgagcgcc tcctgcggcg ccggcgaccg gtcgatgacg
     2161 tggacggagc cgcccatccg ggtcacctgg aatagctcgc gcgcgaaggc gagcggcagg
     2221 cgcacgcagc cgtgggaggc cgggtggccg gggatctggc cggcgtggag cgcgatcccg
     2281 tcccaggtca gccgctgcat gtagggcatc ggcgcattgt tgtacttgtt ggagaagtgg
     2341 cggacgcgct tctgcaatat gtcgaacttg ccggtcggcg tgcggtggcc cggcttgccg
     2401 gtggagaccg tggtcactcc gatcagggtg ccgccgcgat agacgtaggc gcgctgaagc
     2461 gggatcgaga cgaccatctc gacctccccc gccggcgccc gctcgggctg ccagacgaac
     2521 tcgcccggct tgagctccgc ggcttcgacg gcggcggcgg ccgagcgcac ggcggcggcg
     2581 gtcgcctcga cgacgacggc gaccggaccc gagccctccc gggcggcggt ctcggcggcc
     2641 tcggccgggc ggacgggggt gaaggcgacc agcagcgcgc tggcggcaag gaaggcggtg
     2701 cggacggcga acgggttcat gacggcgact ctttactggc gggctggccc gcttggatcc
     2761 ggccggaagc tgaccgtggc cgccgtctat tccccgtcca tggccgcgct gtgccgcgcc
     2821 gccggcttta tggtttcgat tgttgcaatg cctcgaaccg ccgccccgcg gaggcggaat
     2881 gactgtcgcc ggtgacggtc gctttcggct acagcaccgt gatccgcggc tgcgcccgcc
     2941 gcttcctgac cagcagttcg gtcacgccca gaccatcgcg tcggcccggt cgggcgatcg
     3001 acccggcccc tggtagcggc gcccgtagct gagccccgca agctcggcgt cgagctccgg
     3061 gaagcgcccg gcgagcttcg cgcggctcaa agcgcaggca ttgaagcgtg ccttgcgatt
     3121 tgcctgagag gcgcgcttga cgcctgacgg atcatcgagt tcgatcatgg gtactctcca
     3181 aatggaggcc tcaagatcga cgctccgaac caagctccgg gcaatcaccg tcttttacac
     3241 tttgggcggc aggtgacggg acagtgttcg cgaacaggct ccttatcgag tgagctgcaa
     3301 ctctttaggc acgatccagc gacaggctac caatgtttgc tgcaccaagt gcaaccaaaa
     3361 atcctccgct gtccgtcttc gcgcgaacga ccgaggccga gcgtgggtgc cagcgacgtg
     3421 cctggcagcg accggctccc cagcctatgg ggacacgtac cttttggccc aaaaggtacg
     3481 tgtcccccat gctccacccc tgggggccaa gccgggttat cacccgaagc accgaagcac
     3541 cgaagggatc gagcgacgcc gggtcgcctc cattcagacg agccgggaag caattcagac
     3601 aagccggtcc agggagcgcc tgcgcgctcg gccatcctcg tcggccaggt tcggcccgat
     3661 cgccgccaga gcgccttcgg gccttcggtg cttcgggtga agccgggccc cgccggcccc
     3721 gctccgccgt caataatgca ccgcccgccc ataagcgtcg agcacgctct cgtgcatcat
     3781 ctctgacagc gtgggatgcg ggaacaccgt gtgcatcagg tccgcctccg tcgtctcgag
     3841 ctgccgcgcc accgcatagc cctggatcag ctccgtcacc tcagccccca ccatgtgggc
     3901 gcccaataat tcccccgtct cggcgtcgaa caccgtcttg acgaagccct ccgcctcgcc
     3961 gagcgcgatc gccttgccgt tgccgatgaa ggggaacttg ccgacgcgga ccttgcggcc
     4021 ctgctccttc gccttcgcct cggtcagccc gacgctcgcc acctgcggcc gcgaataggt
     4081 gcagcccggg atgttccagg tctcgaacgg atgcggcttg ccgccggcga tcgcctcgac
     4141 gcagaccacg ccctcgtggc tcgccttgtg cgccagccag ggcgggccgg tgacgtcgcc
     4201 gatagcgtag atcccctcga cattggtccg cccatagccg tcgacgacga tatggccgcg
     4261 ctcggtcttc acgcccagcg cctcgagccc gatcccctcc gtattgggca cgatcccgat
     4321 cgcgacgatg gcatggctga attcccggac gtcgccgccc ttcagcgtcg ccttcacccc
     4381 gtccggcccg ctctccaatt tctcgacccc cgaggagacg tggatcttca tcccctgctt
     4441 gactagcgct ttgtggacga aggcgctgac gtcctcgtcc tcgaccggaa ggatgcggtc
     4501 gagcatctcg atgatcgtca cctcggcgcc catgtccgaa tagaagcttg cgaactcgac
     4561 cccgatcgcg cccgagccga tcaccagcag cctggtcggc atgaccttgg gcaccatggc
     4621 gtgccggtag gtccagatgc gctccccgtc ggccttggcg aagggcaggt cgcgggcgcg
     4681 ggcgccggtc gcaacgatga tgtgcttggc ctgaagcgcc ttgtcgccga ccttcaaggc
     4741 ccccttgccc ttcaatgcgc cctcgccctc gaccaccgtc accttgttct tcttcatcag
     4801 ccccttgacg ccggcgttca attggccggc gaccttgcgg ctccggtcga cgatcctcgc
     4861 gaggtcgaag ccgggcttct cggcgacgag gccgtaggcc gaggcgtggg tcatgtagtg
     4921 gtagatctcg ctggtgcgca gcagcgcctt ggtcgggatg cacccccagt tgaggcagat
     4981 gccgcccagc ttctcgcgct cgacgatcgc caccttcatt ccgagctggg aggctcggat
     5041 cgccgcgaca tagccgccgg gcccggagcc cagcacgatg aggtcgtagg agtcagccat
     5101 tgtctttcct tggatggtcg gcgagcagcg gccacagggc ggccgaggcg aggccggcgg
     5161 cggcgcccgt ggcgagcagc gtgcggtcgc cggcggcggc ggcgaatcct cccagcgagg
     5221 cgccgatgac gagcgcggcc aggcttccgg cgagcaggcc ggcggggagc aggacggcgg
     5281 ggcggcggtc gagcccgagc cggcgcagcg cgagccacag cggcaggccg aggagggcgc
     5341 cggcggcgag cgtcgccagc caggccgcga gcaggatggc ggcaacgaac aggggctcgc
     5401 ggacgaggcc ggcgctcggg tccgggctcc ggccgatctg ccaggtcagg aagccgccca
     5461 ctccggccgc gaccggcagg gaggtcagca gataggcggc ggcgcggcgc atgtcagccg
     5521 cgcccggccg gcgggcggtg cttcatcgct ccgcccagga caggaacagc caggcgaaca
     5581 gggtcaccgc cgcgaacaaa gggagcgacc agccgagcgg cgtgtcgccg agggtgaagc
     5641 ggacgaggcc gaggcgcgac cattcgccga gctggcccat gtcgttgcgg aacacgtagg
     5701 ggatggcgag cgaggcgacg agtccgccga ggacggcttt gccgagcgcc atgtcaggcc
     5761 agcattgcca agggggcctc gaccaggcgc ttgaaggcct gcatcagtcg ggcgccgtcg
     5821 gcgccgtcga tggcgcggtg gtcgaagctt ccggtggcgg tcatcatcgt cgcgaccccg
     5881 agcgcgccgt cgaccaccca gggcttcttc tcgccggcgc cgatcgccat gatcatgccc
     5941 tgggggggat ttatgaccgc ggagaagacc gagattccat acatccccat attagagagg
     6001 ctcgccgtcc ccccttgata ttcctcgggc ttgagcttgc cctcccgggc tcgggcggcg
     6061 aggtccttca tttcggtcgc gatccgcgaa agcggcttgg tgtccgcctc aacgatgacc
     6121 ggcgtgatca ggccgttggg gatcgacacc gcgacggaga tgtcggcgcg cttgaacgtg
     6181 atcagctccg tgccggtata ttggacgttg cactcgggca cttcgatcag cgaggcggcg
     6241 agcgccttga tcagcaggtc gttgaccgaa agcttgaccc cgcgcggctc gaggctcgca
     6301 ttgagctcgg ccctgagctt gagcaaagcg tccagccgga tgtcgacggt gaggtagatg
     6361 tgcgggacgt tctgcttcga ttcggtcagt cgccgagcga tcgtcttgcg catgttggag
     6421 agcttggcga cctcgtgcgg gatgtcggga acgccgtagc tcggcgcggc ctccgccggc
     6481 gcctggacgg aagcctgggc cggggccgga gcgccggcgg ccttgccggc cgcgccgtcg
     6541 acgtccgcct tgacgatgcg gccgcccggg ccggagcccg ccaggccggc gagatcgatg
     6601 ccccgctgct cggcgaggcg gcgggccagc ggactcgcct tgatccggcc gccggcctcg
     6661 gccccggcct gcggcgcgag ctcggaatgc tggaccggat tggggggctt gggcgaggcg
     6721 gcggcaggag ccggcgcggc cacttcccgg gcgggctggc cggcgccggt ctcgccgccg
     6781 tctttcccga ccggctcctt cgccgcctcg gcggcgggtg cggccgcggc ggcatcctcg
     6841 ccctcgccgg cgatcagggc gatgacggtg ccgaccttga cctcgtcagt gccttcggca
     6901 accaagatcc tggcgatcgt gccttcgtcg accgcctcga actccatcgt cgccttgtcc
     6961 gtctcgatct cggcgagcag gtcgccggcc gctacggtgt cgccctcctt gaccagccac
     7021 ttggcgagcg tgccctcctc catcgtcggg ctgagggccg gcatcttcaa ttcgatcggc
     7081 atggcgcgcg aaagctcctg gcggcagtgc ggttaaagcc aaacagcaac agaagggccc
     7141 gggtcaaggc ctgtcctcgg caagccccct ctcggccggc gcccactatc gggccggaag
     7201 cgcctcggcg ccctgcggcc agtctccgcc caggaaagcg gcgatcgctt cgagcgtcgc
     7261 ctccggctgc tcgtaagcgg ggaaatgacc ggcgcctgcg atcaccctga ggcgggcgtt
     7321 cggctgtccc atgacccatt cctcgctgcc ggcaagcggg atgttgtcgc gctcgccatg
     7381 gatgatcagg cgcgggatcg tcacggatgc cagcgagccg cgccaatcat agccgtcgat
     7441 cgacgagaag agcgcgccga aataggccgc gagcgcgtcg ggatgctcgt tgggagaggc
     7501 gcacacgtcc ggaatcagcg cggcgcgggc cgaatcggcg agcagcggcg gcaccgaaac
     7561 cgcgttggac tcgcggcatt gctcctcggg ctttcccgca aaggcgccgg aacggacccg
     7621 atcctcgtag gtggccatgg ccgctgcgtc ggtccgggtg cggcggtcgg ccatcatctg
     7681 cgccccatag ggcacgaagc gcggcgccac cggcgcgagc tgcaccagcc gcgtgacgcg
     7741 gcccggatgg cgcatggcgt agacgaaggt ctccatgccc gcgcccgacc agccgatgat
     7801 cgcgacccgc tccgcgccga ccgcccgccg gacggtgtcg agatcgacca gcagcgcgtc
     7861 gagcgaaacc ttggccgggg gcaccgccgc cgaacggccc cgcccgcgcg ggtcgtaggt
     7921 gacgattcgg cgtcccctgg cgagcgggtc gagcgagcgg ccgtgataga gcgcgaacgg
     7981 agcgatcacg accgcgtcgc cggtgcccgc gacccggtaa tgaaggcgca cgccgtccgg
     8041 agtcagggcc tcgccttcgg cgaccggaag cgaccggagc tcgccggcgg agagcagcgg
     8101 gccgacggcg gagctggggg cggcgcaagc cgaaaggacg aaggccgccg ccgcggcgag
     8161 gggaaggagg atcttcatcg acgtccagcc tcatgccgtg tccggcgcga ccctaggtcc
     8221 gatccgaatg gccggcaacg tttccgccgc agcccgcagg atcttcctcg gacttccttt
     8281 ggcgccgggc acggtttcga ccgtccttgc gcgcccgtcc gatctggcgc agcatcgctg
     8341 ccggaacggg ggcccgatgc gatcctatct ggtggtgatc gacgagacga gcgaggcgcg
     8401 tgccgccctt cgctacgccg cgcgccgcgc ctccgggacg gggggcggag tcatcctgct
     8461 cgccgtcatc ccgcccgccg agttcgtcca gtggggcgga gtccaggcgg cgatggagga
     8521 ggaggcgaag ctgcgcgccg aggcgatggt gctccaggcc tccggcgcga tcgtcgagga
     8581 ggccgggatc gagccgacca tcctcgtccg ccagggcgag cccgagaagg agatcgccga
     8641 cctgctcgcc gagcgcgacg acgtcgccgc cctcgtcctg ggcgcggccg aagagggcgg
     8701 ccccggcccg ctggtcagcc atttctccgg cgccgtcgcc ggcagcctgc cctgtccgct
     8761 ggtcatcgtc cccggccgcc tc
//
LOCUS       DASVQZ010000329         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_358956, whole genome shotgun sequence.
ACCESSION   DASVQZ010000329 DASVQZ010000000
VERSION     DASVQZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358956"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VF547_09215"
     CDS             <1..983
                     /locus_tag="VF547_09215"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX8263039.1"
                     /translation="LTRKVLRVPATHRIGIVPASDTGAVEMALWSLLGARPVTLLAWE
                     SFGEGWVTDVVKQLKLEATVIKAPYGALPDLAAVDPASDVVFTWNGTTSGVKVPNGDW
                     IGEAREGLTIADATSAVFAQDIPWAKIDVATFSWQKVLGGEGGHGVLILGPRAVERLD
                     SYTPPWPLPKIFRLTKSGKLIEGLFHGETINTPSMLAVEDYIFALEWALGIGGLAGLM
                     ARADANAAAVDAWVERTDWIEHLAADPAIRSNTSVCLKFAGGGDAEAQAALVKRMAAL
                     LEAEGAGYDVASYRDAPPGLRLWCGATVDTADLEALFPWLDWAWHEARSA"
     gene            1062..>2289
                     /gene="serA"
                     /locus_tag="VF547_09220"
     CDS             1062..>2289
                     /gene="serA"
                     /locus_tag="VF547_09220"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX8263040.1"
                     /translation="MPKVLISDPMDPKAAEVFRANGVEVDEKPGLSKDELKAIIGGYD
                     GLAIRSATKVTADLLDGGSRLKVIGRAGIGVDNVDIAAATARGIVVMNTPFGNSITTA
                     EHAIALMFALARDLPAADSSTQAGKWEKNRFMGVELTSKTLGLIGAGNIGSIVADRAL
                     GLKMKVVAYDPFLTPERAQELGVEKVDLDALLARADFITLHTPLTDQTRNILSRENLA
                     KTKKGVRIINCARGGLIDEAALKEALDSGQVAGAALDVFVEEPAKASPLFGTPGLVAT
                     PHLGASTTEAQVNVAIQVAEQMSEFLTRGGVTNALNMPSLSAEEAPRLRPYMELAEKL
                     GSVVGQLEGHHATGIAIEVEGAAAQLNQKPITGAVLAGLMRVYSDTVNMVNAPTLAKD
                     RGLDVREVRHDREGDYH"
BASE COUNT          382 a          798 c          781 g          328 t
ORIGIN      
        1 agctgacgag gaaggtgctg cgagtccccg cgacccaccg catcggcatc gtccccgcct
       61 ccgataccgg cgcggtcgag atggcactct ggtcgctgct cggcgcgcgg cccgtcacgc
      121 tcctcgcctg ggagagcttc ggcgagggtt gggtgaccga cgtcgtcaag cagctcaagc
      181 tcgaggcgac ggtgatcaag gcgccttatg gcgcgctgcc cgacctcgcc gccgtcgacc
      241 cggccagcga cgtcgtcttc acctggaacg gcaccacgtc cggagtgaag gtgccgaacg
      301 gcgactggat cggcgaggcg cgcgaggggc tgaccatcgc cgacgccacg tctgcagtgt
      361 tcgcccagga tatcccctgg gccaagatcg acgtcgccac cttcagctgg cagaaagtgc
      421 ttggcggcga gggcggccac ggcgtgctga tcctcggccc gcgcgcggtc gagcggctcg
      481 atagctacac gccgccctgg ccgctgccca agatcttccg gctcaccaaa agcggcaagc
      541 tgatcgaggg cctcttccac ggcgagacga tcaacacgcc gtcgatgctg gcggtcgagg
      601 attatatctt cgccctggaa tgggcgctgg gcatcggcgg cctcgccggg ctgatggcgc
      661 gcgcggacgc caatgccgcc gcggtcgacg cctgggtcga gcgcaccgac tggatcgagc
      721 atctcgccgc ggatcccgcg atccggtcca acaccagcgt ctgcctcaag ttcgccggcg
      781 gcggcgatgc cgaggcgcag gcggcgctgg tcaagaggat ggcggcgctg ctcgaggcgg
      841 aaggggccgg ctacgacgtc gccagctatc gcgacgctcc gccgggtctc aggctgtggt
      901 gcggagcgac ggtcgacacc gccgacctcg aggcgctctt cccctggctc gactgggcct
      961 ggcacgaggc gcgctcggcc tagcccgcaa tgctcaccgg cgaaggccgg tgcccagccc
     1021 tttttctgga ccccggcctt cgccggggaa cgaggtgata aatgcccaaa gtcctgattt
     1081 ccgacccgat ggaccccaag gccgcggagg tcttccgcgc caatggggtc gaggtcgacg
     1141 agaagcccgg cctgtcgaag gacgagctga aggcgatcat cggcggctat gacgggctcg
     1201 ccatccgctc cgccaccaag gtgaccgccg acctgctcga cggcgggtcg aggctcaagg
     1261 tgatcggccg ggccggcatc ggagtcgaca atgtcgacat cgccgccgcc accgcacgcg
     1321 gcatcgtcgt catgaacacg ccgttcggca actcgatcac caccgccgag cacgccatcg
     1381 ccctgatgtt cgccctcgcc cgcgacctgc cggccgccga ttcgtcgacc caggcgggca
     1441 agtgggagaa gaaccggttc atgggcgtgg agctgaccag caagacgctc ggcctgatcg
     1501 gcgccggcaa tatcggctcg atcgtcgccg accgggcgct cgggctgaag atgaaggtgg
     1561 tcgcctacga tccgttcctc accccggagc gcgcccagga gcttggagtc gagaaggtcg
     1621 acctcgacgc gctgctcgcc cgggccgact tcatcaccct gcacacgccg ctcaccgacc
     1681 agacccgcaa catcctgtcc cgggagaatc tggccaagac caagaagggc gtcaggatca
     1741 tcaactgcgc ccgcggcggg ctgatcgacg aggcggcgct caaggaggcg ctcgacagcg
     1801 gccaggtcgc cggggcggcg ctcgacgtgt tcgtcgagga gccggccaag gcgagccccc
     1861 tgttcgggac gccgggcctg gtcgcgaccc cgcatctcgg cgcctcgacc accgaggcgc
     1921 aggtcaacgt cgccatccag gtcgccgagc agatgtcgga gttcctgacc cgcggcggag
     1981 tcaccaacgc gctcaacatg ccgtcgctct cggccgagga ggcgccccgg cttcggccct
     2041 atatggagct cgcggagaag ctcggctcgg tggtcggaca gctcgagggc catcatgcga
     2101 ccggaatcgc gatcgaggtc gagggtgcgg cggcacagct caaccagaag ccgatcaccg
     2161 gcgccgtgct cgccggcctg atgcgggttt attccgacac ggtgaacatg gtgaacgcgc
     2221 cgacacttgc gaaagaccgc gggctcgacg tccgcgaagt ccggcacgac cgcgagggcg
     2281 attaccata
//
LOCUS       DASVQZ010000330         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_550009, whole genome shotgun sequence.
ACCESSION   DASVQZ010000330 DASVQZ010000000
VERSION     DASVQZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550009"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..266)
                     /locus_tag="VF547_09225"
     CDS             complement(60..266)
                     /locus_tag="VF547_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263041.1"
                     /translation="MDDKPKISTGPNDRPRNETIAGTGPGIPDDALAPGEEMPQAPSD
                     EEVERAKAALGLGTEKEGLPVEGQ"
     gene            318..686
                     /locus_tag="VF547_09230"
     CDS             318..686
                     /locus_tag="VF547_09230"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX8263042.1"
                     /translation="MSRASDAILAELRAFGLAYPGAHSKAPWPEHDDLAVNDKTFAYL
                     PVAGQPFSLSVKLPYTGEVALDLPYAKPTAYGLGKSGWVSFTPSEEEMPSLDQLKDWI
                     DESYRAQAPRKLVKELDSRD"
     gene            complement(769..1752)
                     /locus_tag="VF547_09235"
     CDS             complement(769..1752)
                     /locus_tag="VF547_09235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8263043.1"
                     /translation="MIPLSVLDLAPVPEGSDTGQALRNSLDLARAAERLGYKRFWMAE
                     HHSMPGIASAATAVALAFVGAGTSAIRIGAGGVMLPNHAPLVIAEQFGTLESLFPGRV
                     DLGLGRAPGTDRAAAVALRRNLASDENQFPGDVLELIAYFDEPKDGRAVRAVPGEGLA
                     IPVWILGSSLFGAQLAAMLGLPYAFASHFAPGQMMEALRVYRERFQPSARLDRPYVML
                     GFNAFAAETKEEAELLVTSVQQAFVNLHAGMPGKLAPPLRGYADSLPLPARALLRQVL
                     SCSAIGDARRVRSEMDAFVERTGADELMIVSQIHDHQARLRSYEIVADAMA"
     gene            1925..3310
                     /locus_tag="VF547_09240"
     CDS             1925..3310
                     /locus_tag="VF547_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8263044.1"
                     /translation="MLQRSPETRRLWRAEIGATAALAWPLILTNIAQALIHVTDVVLL
                     GWAGPSVLAAGTLGVNVYFAFLIFGIGLVTASAPMIAKELGERAHSVRDVRRTVRQTM
                     WAAAALAVPVMAVLWNTGAILVAIGQDPELARAAESFVRALQWGFLPYLWYLVLRSFI
                     SALERPLWSFGIGLAAVIYNGVINYGLIFGKLGLPKLGLLGAGLGSASANLFMFLGMA
                     LVVLLQRRFRRYRLFGRFWRADWSRFREVWRLGLPIAVTLALEITIFNAAVFLMGLIG
                     EESIAAHAIAIQLAALSFMVPMGLSQAATVRVGLAFGRRDRAAVARAGWTAFALGVGF
                     MTMTGLVMIAIPEALVTSFLDPAIPANAPVIRLAVSFLVVAAFFQIFDGAQVVAAGML
                     RGLHDTRVPMIYAALGYWVIGLVTGVGLGFGLGWEGVGIWIGLAAGLGAVAVMLLVRW
                     LRRERLGLTDW"
     gene            3373..3449
                     /locus_tag="VF547_09245"
     tRNA            3373..3449
                     /locus_tag="VF547_09245"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3407..3409,aa:His,seq:gtg)
     gene            3480..4526
                     /locus_tag="VF547_09250"
     CDS             3480..4526
                     /locus_tag="VF547_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX8263045.1"
                     /translation="MQAPWDYPDFDDHEIVHFVTDRASGLCAVIAVHSTFLGPAGGGV
                     RFWHYAQPGEAVRDALRLSRGMSFKNAMAGLPLGGGKAVILADEQRTKTPAMLAAYGR
                     SVEGLGGRYVTAEDVGMSVADMVALSKETSHVAGLPVAEGSVGGDPGPHTSYGVFLGV
                     KAAVRRALGKDSLAGVHIAIQGAGSVASGLARRAAAEGARITLTDIDRSRAEALAREV
                     GGETAAPETIMTLEADVLSPNALGAILTEESIAALRVPVVAGGANNQLATPEDGQRIH
                     QRGILYAPDYVINAGGIINVSTEYLKDGDQQTVNGRIEQIPIRLEQIWSESAATGRNP
                     AEVADAMAQRLIGR"
     gene            complement(4584..5510)
                     /locus_tag="VF547_09255"
     CDS             complement(4584..5510)
                     /locus_tag="VF547_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8263046.1"
                     /translation="MPWGEAMSSDRPRQNRLLGIGLRVAATSCFAFLAMLVKLGHEAG
                     VETVELAFYRFAFGLPPLLLWIAASRSFGAWRTERPLAHLWRGTIGLTTLVLAFSALA
                     FLPLAEATTIGFAAPLFAVLLSALVLGEPVGRYRWSAVAAGFVGMLVVMRPEARHLPA
                     AGLMLAALAAFGAGIVTITIRQIGRTESTQTTVLWFSLFSIAVTGAAMPFFAQPHDGG
                     TWLLLLALGVVGGTSQICLTASLRYAPVAVVAPFDYVQLLWSVLLGWLIFAQQPMATT
                     WAGAAIIMASGLYTLYREHRLGREQAREAAAL"
BASE COUNT          825 a         1946 c         1957 g          853 t
ORIGIN      
        1 cacgggtggg gtcgtaggtt caatccctac cgcgcccacc acgcccgctc cgcgttccgt
       61 cactggcctt cgacgggaag cccctccttc tccgtgccga ggccgagcgc cgccttcgcc
      121 cgctcgacct cttcgtcgct cggggcctgc ggcatttcct cgcccggagc cagagcgtca
      181 tccgggattc ccggaccggt tccggcgatg gtctcgttgc gcgggcgatc gttgggaccg
      241 gtgctgatct tgggcttgtc gtccatgcga acttctcctc gctgtgctaa tgacggcgcg
      301 gcaaggaggt tccgacgatg agccgagcga gcgatgcgat cctggcggag ctgcgcgcct
      361 tcgggctggc ctatcccggc gcccattcca aggccccctg gcccgagcat gacgatctcg
      421 ccgtcaacga caagaccttc gcctatctgc ccgtggccgg ccagccgttc agcctctcgg
      481 tcaagctccc ttataccggc gaggtcgcgc tcgacctgcc ttatgcgaag ccgacggcct
      541 acggcctcgg caagtcgggc tgggtcagct tcacgccgtc ggaagaggag atgccgagcc
      601 tcgaccagct caaggactgg atcgacgaga gctaccgggc gcaggcgccc aggaagctgg
      661 tcaaggagct ggattcgcgg gactgacgtc atccggcgaa agccgggacc tcgtggagac
      721 ctgcggagac cgcttgtcgc aatgccccgc tttcaccgcg gtgacgaatt acgccatcgc
      781 gtcggcgacg atctcgtagg agcgcagccg ggcctggtgg tcatggatct gcgacacgat
      841 catcagctcg tcggcgccgg tccgctcgac gaaggcgtcc atttccgacc tcaccctgcg
      901 cgcgtcgccg atcgcagagc aggacaggac ctggcggagc agggcccgcg ccggcagcgg
      961 cagggaatcg gcatagccgc gaagcggcgg cgcgagcttg ccgggcatgc cggcgtggag
     1021 gttcacgaaa gcctgctgga ccgaggtgac gaggagctcg gcctcctcct tcgtctccgc
     1081 cgcgaaggcg ttgaagccga gcatgacgta gggccggtcg agccgcgccg agggctggaa
     1141 gcgttcgcga tagacccgga gcgcctccat catctgcccg ggcgcgaaat gggacgcgaa
     1201 ggcgtaggga aggccgagca tcgccgcgag ctgggcgccg aacaggctgg agccgaggat
     1261 ccagaccggg atcgcgagac cctcgccggg cacggcgcgg accgctcggc cgtccttcgg
     1321 ctcgtcgaaa taggcgatca gctcgaggac gtcgccgggg aactgattct cgtccgaggc
     1381 gaggttgcgg cgaagcgcca cggcggcggc gcggtccgtg cccggcgccc gcccgaggcc
     1441 gaggtcgacc cggcccggga aaagcgattc gagggtcccg aattgctcgg cgatgaccag
     1501 cggcgcgtgg ttggggagca tcaccccgcc ggcgccgatg cggatggccg acgtgccggc
     1561 gccgacgaag gcgagcgcga ccgcggtcgc ggcgctggcg atgcccggca tcgaatgatg
     1621 ctcggccatc cagaagcgct tgtagcccaa tcgctcggcg gcgcgggcga ggtcgagcga
     1681 gttgcgcagc gcctggccgg tatcggaacc ttccgggacc ggggcgagat cgaggactga
     1741 gagtgggatc atgggcggga gatggtgtcc cgctccgtgc cgcaccagcc ccgtcgttgc
     1801 cggagcgaag cggcgcggcg atccggctcg gagtctgtct cgaacccagc gctggattgc
     1861 cctcgcagcg ctcgcaatga cggtgcttcc acgtccagcg atcaggctct acggcccttg
     1921 ccctatgctt cagcggagcc ccgagacccg tcgcctctgg cgcgccgaga tcggcgccac
     1981 ggcggcgctc gcctggccgc tgatcctgac caacatcgcc caggcactga tccatgtcac
     2041 cgacgtcgtc ctgctcggct gggcggggcc ctcggtgctc gccgccggaa cgctcggcgt
     2101 gaacgtctat ttcgccttcc tgatcttcgg catcggcctg gtcaccgcct cggcgccgat
     2161 gatcgccaag gagctgggcg agcgcgccca cagcgtccgc gacgtccgcc ggacggtgcg
     2221 ccagacgatg tgggcggcgg ccgcgctggc cgtgccggtg atggccgttc tgtggaacac
     2281 cggcgcgatc ctcgtcgcga tcggacagga ccccgagctc gcgcgggcgg cggagagctt
     2341 cgttcgagcg cttcaatggg gcttcctgcc ctatctctgg tacctggtgc tgcgctcctt
     2401 catctcggcg ctggagaggc cgctctggtc gttcggaatc ggccttgcag ccgtgatcta
     2461 caacggagtg atcaattacg gcctgatctt cggcaagctc ggcctgccca agctcggcct
     2521 gctcggagcg ggcctcggca gcgccagcgc caacctgttc atgttcctcg gcatggccct
     2581 ggtcgtgctc ctgcagcgcc gcttccgccg ctaccggctg ttcggccgtt tctggcgggc
     2641 ggactggtcg cgcttccgcg aggtgtggcg gctcggcctc ccgatcgccg tcaccctcgc
     2701 gcttgagatc accatcttca acgccgccgt cttcctgatg ggcctgatcg gcgaggaatc
     2761 gatcgccgcc cacgccatcg ccatccagct cgcggcgctg agcttcatgg tgccgatggg
     2821 actgtcgcag gcggcgacgg tgcgggtcgg gctcgccttc ggccgccgcg atcgcgccgc
     2881 cgtcgcccgc gccggctgga ccgccttcgc gctcggggtc gggttcatga cgatgaccgg
     2941 cctcgtcatg atcgccattc ccgaggcctt ggtcacctcg ttcctcgacc cggcgatccc
     3001 cgccaacgca ccggtcatcc ggctcgccgt ctcgttcctc gtcgtcgccg ccttcttcca
     3061 gatcttcgac ggcgcgcagg tggtcgcggc cggaatgctg cgcggcctcc acgacacgcg
     3121 cgtgccgatg atctatgcgg ccctcggcta ttgggtgatt ggcctcgtca ccggagtcgg
     3181 gctgggcttc ggcctgggct gggagggggt cgggatctgg atcggcctcg ccgccgggct
     3241 cggcgcggtc gcggtcatgc tcctcgtgcg ctggcttcgg cgcgagcggc tcggccttac
     3301 cgactggtga gggtcccttg acgcatctgc gccgcggccc ctatgtgcgc gccgcacctt
     3361 caggcgcttg cggcgatcgt agctcagctg gttagagcat cggtttgtgg taccgagggt
     3421 cgcgggttca agtcccgtcg atcgccccac cgcgcctgcc tcgttttcag gatccttgca
     3481 tgcaagcccc gtgggattat cccgatttcg acgatcatga gatcgtccac ttcgtcacgg
     3541 accgcgccag cggcctttgc gccgtcatcg ccgtccactc gaccttcctc ggaccggccg
     3601 gcggcggagt gcgcttctgg cattatgcgc agcccggcga ggcggtgcgc gacgcgcttc
     3661 ggctgtcgcg cgggatgagc ttcaagaacg ccatggccgg cctgcccctg ggcggcggca
     3721 aggccgtcat cctcgccgac gagcagcgca ccaagactcc cgcgatgctc gccgcctacg
     3781 gccgctcggt cgagggcctg ggcgggcgct acgtcaccgc cgaggacgtc ggcatgagcg
     3841 tcgccgacat ggtcgccctg tcgaaggaga cgagccacgt ggcgggcctg ccggtcgcgg
     3901 aaggttcggt cggcggcgat ccgggacccc acaccagcta tggcgtcttc ctcggcgtca
     3961 aggcggccgt ccgccgcgcg ctcggcaagg acagcctcgc gggggtccat atcgccatcc
     4021 agggcgccgg cagcgtcgct tccggcctcg cccgccgggc cgcggccgaa ggcgcccgga
     4081 tcaccctcac cgacatcgac cggagccgcg ccgaagcgct ggcccgcgag gtcggcggcg
     4141 agacggccgc gccggagacg atcatgaccc tggaggcgga cgtcctcagc cccaacgcgc
     4201 tcggcgcgat cctcaccgag gaatcgatcg cggcgctccg cgtgccggtc gtcgcgggcg
     4261 gcgccaacaa ccagctcgcc acgcctgagg acggacaaag gatccaccag cgcggaatcc
     4321 tctacgcccc cgattacgtg atcaacgcgg gcggcatcat caacgtctcg accgaatatc
     4381 tgaaggacgg cgaccagcag acggtaaatg gacggatcga gcagattccc atccgcctgg
     4441 agcagatctg gtcggaaagc gcggcaacgg ggcgcaaccc cgcggaagtc gccgacgcga
     4501 tggcgcaaag gctgatcggg cgctgacggc tgaggcgccg cttccgtcgt ccgcccggac
     4561 cagagctcgg cgctgcttcg cctctagagg gcggcggcct ccctcgcctg ctcccgtccc
     4621 agccggtgct cgcggtagag ggtgtagagg ccgctcgcca tgatgatcgc cgcgcccgcc
     4681 caggtcgtcg ccatcggctg ctgggcgaag atcagccagc cgagcaggac cgaccagagc
     4741 agctggacat agtcgaacgg cgccacgacg gcgacgggtg catagcgcag cgaggcggtg
     4801 aggcagatct ggctcgtgcc cccgacgacg cccagcgcga gcaggagcag ccaggtgccg
     4861 ccgtcgtgcg gctgggcgaa gaagggcatc gccgcgcccg tgaccgcgat cgagaagagg
     4921 ctgaaccaca gcaccgtcgt ctgggtcgat tcggtccgtc cgatctggcg gatggtgatg
     4981 gtcacgattc cggccccgaa ggcggcgagg gccgcgagca tcagccccgc ggccggaagg
     5041 tggcgggcct cgggccgcat caccacgagc attcccacga atccggcggc gaccgcgctc
     5101 cagcggtagc ggccgaccgg ctcgccgagg acaagcgccg agagcaggac cgcgaacagg
     5161 ggcgcggcaa agccgatcgt ggtcgcttcg gcgagcggca ggaaggcgag ggcgctgaag
     5221 gcgaggacca gcgtcgtcag gccgatcgtg ccgcgccaca gatgggcgag tggccgctcg
     5281 gtccgccagg cgccgaaact gcggctggcc gcaatccaca aaagcaaggg cggcaggccg
     5341 aaggcgaaac ggtagaaggc cagctccacc gtctcgacgc cggcctcgtg gccgagcttg
     5401 accagcatcg ccaggaacgc gaagcagctc gtggcggcga cgcgcaggcc gatgcccaga
     5461 aggcggttct ggcggggccg gtccgacgac atcgcttcgc cccaaggcac gctggccgtt
     5521 gaaaaggcaa ggcccggcgc gggggccgcc ggagctgggc gagtggcttg ccaccacccg
     5581 g
//
LOCUS       DASVQZ010000331        16758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_68708, whole genome shotgun sequence.
ACCESSION   DASVQZ010000331 DASVQZ010000000
VERSION     DASVQZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68708"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF547_09260"
     CDS             complement(<1..213)
                     /locus_tag="VF547_09260"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX8263047.1"
                     /translation="MRIGLLTGGGDVPGLNPCIRSITLSADALGWQVTGFRRGWEGVL
                     AIDPGDPASVAASSLPLTREGVRGIDR"
     gene            complement(218..943)
                     /locus_tag="VF547_09265"
     CDS             complement(218..943)
                     /locus_tag="VF547_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX8263048.1"
                     /translation="MILSCPACKTRYVVPDSAIGPSGRQVRCASCRHSWLQPPPPLAA
                     RPAAEWKPPPSAAESPPPPLREPAPVRTAASMLGPAPTPAAEEYDAFAHEPPFRPRRN
                     RARMWTIIAIFAAALMLAATAALYSFDLPALGVDIALPGSSATPLQVEDVRAQNNTLA
                     SGNALLTVTGRISNPTQSVQRVPQLRAEVREQDGKTVVYSWSISPPVSDLQPGQSATF
                     NSAQMDVPTAPGRKLRVRFPSSG"
     gene            1103..1801
                     /gene="ftsE"
                     /locus_tag="VF547_09270"
     CDS             1103..1801
                     /gene="ftsE"
                     /locus_tag="VF547_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX8263049.1"
                     /translation="MVQFDNVGLRYGTGAETLSDLSFSLRQGGFYFLTGPSGAGKTSL
                     LKLLYLAQRPSRGLIKLFDEDIVTMPRKRLPGFRRRIGVVFQDFRLVHHLSAFDNVAL
                     PLRVAGVDEKDLQGPVGEMLAWVGLGHRASARPATLSGGEQQRVAIARAVIARPELLV
                     ADEPTGNVDPEMAKRLLHLFDSLNKLGTTVVVATHDLHLISSIGRSEMLRLDRGTLLD
                     PTGALRHPPRRADA"
     gene            1870..2688
                     /locus_tag="VF547_09275"
     CDS             1870..2688
                     /locus_tag="VF547_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8263050.1"
                     /translation="MPWVIAIMMFLTVLAAAAGLGLGGAAASLGAGLGNRVTVQVLEP
                     NPDRREAEAARALDRLRRLPEVESVRRLSPEEMKDLLEPWLGAGGLEGDLPIPAMIDA
                     QLIPGARADTLRGAVTAAAPSARIDDNDQWLAPLGRLIAALKWLALGLVALMIGATAA
                     TVVLAARAALDTHRGTIEVLHLMGATDVQVARLFQRRIALDALFGGVVGFAAAALALL
                     VIGRRIGALGSDLLGSANLPATSWLALAALPVLGVGLAMLVARATILRALGRML"
     gene            2685..3215
                     /locus_tag="VF547_09280"
     CDS             2685..3215
                     /locus_tag="VF547_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX8263051.1"
                     /translation="MIRRALSAVLLVYLLGYAVFAVLLPRPADERRTDAIVVLTGGAK
                     RIERGLDLMERRLAKRMLVSGVARTVRPEELAAQYQADSALFGCCVDLGRESVDTRSN
                     AEEVARWMDRHKFRTMRLVTTDWHMPRARFELSRRLGGGIAVTGDAVESNPSFGQLFT
                     EYNKYLLRRAAVLVGI"
     gene            3215..3898
                     /locus_tag="VF547_09285"
     CDS             3215..3898
                     /locus_tag="VF547_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8263052.1"
                     /translation="MNLVRSLLYALVFYPGSVLYVLAGIAMIPLGRGAVRRVADAWAG
                     FNRLCARFLLGITSRVEGEVPLGPALVAVKHQSMFETLEIALLLDTPATVMKSELGRI
                     PLWGRLTRVYGILPVDRDGGGAALRKLLRAADEAKAEGRPIVIFPEGTRVAPGESPPL
                     EPGFAGLYRALGLPVVPVALDSGKVWPRRSFVKRPGVVTIRFGDPIPPGLPRREAEAR
                     VHAAINALE"
     gene            complement(4280..5335)
                     /locus_tag="VF547_09290"
     CDS             complement(4280..5335)
                     /locus_tag="VF547_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEX8263053.1"
                     /translation="MPGSATEEAVRAAFARQAHWAEALGSPFMVRLCRLLGERLDRKT
                     ELGRRVLDWPGEPDSLADALPLRLTGGLHALVRRGRAPGLAACYPPRPLPDEEALWAA
                     VRPVLGDPELMPWLDGPPQTNEVGRSAVLMAGLLVVADLFPQPVELLELGASAGLNLL
                     LDRYGYDLGGLRAGDPASPLQLKPDWMGDRPPAARVEVAGRRGVDLRPLDPRRDGERL
                     IAYVWPDQRRRLAQLEAALAIARAEPAPVERGDAAGWLESRLAEPAAAGVTRVVFHSI
                     AFQYFDEASKARIAAAMARSGAAAAAGRPLAWLRYEHDPRDERLTLRLRTWPGQESLL
                     AYAHPHGSLVHWLDGDD"
     gene            complement(5316..7694)
                     /gene="pheT"
                     /locus_tag="VF547_09295"
     CDS             complement(5316..7694)
                     /gene="pheT"
                     /locus_tag="VF547_09295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8263054.1"
                     /translation="MKFTLSWLKEHLDTDADLQTIVGTLTAIGLEVEGVENAAETLAP
                     FTVARILTAERHPQADKLQVLTVDAGTGEAVQVVCGAPNARAGMLGVFGAPGAYVPGS
                     DLTLKIATIRGVESRGMMCSVRELELGDEHDGIIELPADAPVGAGYAQWAGLDDPVIE
                     VNVTPNRQDCMGVHGIARDLAAAGLGALKPVRTREIEGSFPCPVEIRIEDPEACPAFY
                     GRVVRGVRNGPSPDWLQRRLKAVGQRPISALVDMTNYVAITYGRPLHVYDLAKLEGAV
                     VARRARDGEEMTALNGKSYRLDPSMTVIADARGVHDIGGIMGGEHSGVTEATSDILIE
                     CAYFDPKSIARTGQKLGLASDARTRFERGVDPEFVGPGLDIATELAIELAGGEPSEIV
                     RAGEPPKLEKSIQYRPARARELGGLDVPEPRQKEILERLGFTVLEGEQWTIIVPSWRR
                     DIDGAADIVEEVARIEGFDKVEATVLPRAAGVARPTATPDQLIERRARRTAAARGLNE
                     AVTWSFVSESEADAFGGSSWILANPISEEMKAMRPSLLPGLLAAARRNADRGQSGVRL
                     FELGRRYLADGERPTLGLVLAGERAPRHWRTGKAQDFDAYDAKAEALAILAAAGAPAD
                     SLQSLAGAGPVYHPGRSARLALGPKTVLAEYGELHPATLKAFDLDGPAVAAEIYLDAI
                     PARRGGGGRMREAYAPPALQAVKRDFAFLVAAEVPAETLLRAVRGADRQAIAAVSLFD
                     VFTGPGVPEGLKSLAVEVVLQPGEKSFTEEDLKAVSGRIVAAAAKAGARLRD"
     gene            complement(7819..8910)
                     /gene="pheS"
                     /locus_tag="VF547_09300"
     CDS             complement(7819..8910)
                     /gene="pheS"
                     /locus_tag="VF547_09300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8263055.1"
                     /translation="MADLDRLSGDLLARISSAADLAGLEAERVAALGKQGAVTQLLKT
                     LGAMTPEERQAEGPRIHGLRERVAEAIAGRKAALEAEALERRLATEGLDLTLPVAPPA
                     QGSVHPVSQVLDELAEIFADLGFSVATGPEIEDDWHNFTALNFPPDHPARAMHDTFYL
                     RQRGEGGDGAEEPMLLRTHTSPVQVRTMQSQAPPIYIIAPGRTYRSDNDATHTPMFHQ
                     IEGLVIDKGIHLGHLKWTLETFLKAFFEREDIVLRLRPSYFPFTEPSAEVDVGFTRVN
                     GRRVLGGSEGWMELLGSGMVHPRVIAACGLDPDEWQGFAFGCGVDRLAMLKYGMDDLR
                     AFFDGDLRWLKHYGFAALDVPTLSGGVGA"
     gene            9278..10207
                     /locus_tag="VF547_09305"
     CDS             9278..10207
                     /locus_tag="VF547_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX8263056.1"
                     /translation="MAWPELSEARDGPTIAALHLYSQVLGKAAVALLPWRNHGWHVTL
                     RVHPRGLRTEPLYGAGGPFELGLDLAGHQFTFADGGGLRRFELAPVPVSRFHAEVMTC
                     LAAAGHEVRIHPATNELDPPVRFADDHVPRAYDRGSASRLLGALLDSDRVFRRFRSGF
                     LGKASPVHFFWGSFDLAVTRFSGRPAPLHPGGIPYLPDEITREAYSHEVSSAGFWPGG
                     AGGGGDPFFYSYAYPSPEGFAAAPVRPEAARFDEALGEFILPYEAVRAAGDPDRALLS
                     FLDSTYAAAADRAKWDRPSLECEPGRPRVPRPL"
     gene            complement(10360..10947)
                     /locus_tag="VF547_09310"
     CDS             complement(10360..10947)
                     /locus_tag="VF547_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263057.1"
                     /translation="MRPFAAAAAALLVLQAPPLAARAAKAPASEATIDRFLAVLPDRG
                     EIDSPSAEVDPAELGALAALNPGKEAQVRSVLEGNLACSNEAISAGTRRMLRTVAGDL
                     GEAKVKRLADFYAGPDYAAFEALGARMNAAATPSAEDQAAMAGLMEAYPLQAWLEGLN
                     RAQEIIAADEGFLTEAMKCAELQMNALEAAGLKSH"
     gene            complement(11060..12469)
                     /locus_tag="VF547_09315"
     CDS             complement(11060..12469)
                     /locus_tag="VF547_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX8263058.1"
                     /translation="MPAPERKLYLGGRLRRLRRELGLNQSAMAGEIGVSPSYLNHLER
                     NQRPVTAQVLLRLAEVYDVDLRAFAAEGADGTGIDQLAEIFADPMFADIGIPRFELLE
                     VADNAPSVADAISRLYAALVERRQHPAGAVPDETSPVTPEAWVRDHIQAQRNHFPYIE
                     EAAETLAGALGDPNGIFDPLRRRLREGFGIDTRVVPPELIEPTSQAYDHHRKRLMLSA
                     MLRPESRTFAVAYQLALVEFGPMLKRMVETAGAPDAPTRRLLHMSLANYAAGAIMMPY
                     AGFLAAAEKHRHDVDRLCADFGASVEQISHRLTTLARTGARGVPFFMLRVDAAGNISK
                     RFAGESFPFSRFGGTCPRWNLHAAFQTPGRVATQIVETPDGARFFTLARTVDRAVRLD
                     PRESSQLAIGLGCDARYAPRIVYADGHDLAKPLVTPIGPACTICPRLRCPQRAAAPAG
                     RTLALNETQKTISPYPFVG"
     gene            12600..13886
                     /gene="aceA"
                     /locus_tag="VF547_09320"
     CDS             12600..13886
                     /gene="aceA"
                     /locus_tag="VF547_09320"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX8263059.1"
                     /translation="MSDFTTIVPAPPRRFDGIERPYSPEDVLRLRGSVPVEQSLARRG
                     ANRLWELLARHDHVHSLGAVTGNQAMQMVRAGLKAIYLSGWQVAADANTAGAMYPDQS
                     LYPANAGPELCRRINRTLQRADQIEHAEGGARRDWLVPIVADAEAGFGGPLNCFEIMK
                     AYIEAGAAAVHFEDQLASEKKCGHLGGKVLIPTRAHIRNLVSARLAADVCGVPTLVVA
                     RTDAESARLITSDVDERDREWLSGERTAEGFFRLREGTGIDHCIKRGLAFAPYADLLW
                     WETSNPNLDDARRFAEAIHREYPGKLLAYNCSPSFNWAAKLDKGTIAAFQRELGAMGY
                     KFQFVTLAGFHSLNHGMFELASAYRDRGMAAYSELQQAEFASESLGYTATRHQREVGT
                     GYFDLIAQTVAGGEASTVALAESTEAEQFIAAVAAE"
     gene            13898..14098
                     /locus_tag="VF547_09325"
     CDS             13898..14098
                     /locus_tag="VF547_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263060.1"
                     /translation="MTKRFWVVGGEYEGPDFRALIPGTETMAGPFEDERRARTEWTRL
                     SCCPEKSGRATVRYSIAAETLH"
     gene            complement(14271..14648)
                     /gene="rplT"
                     /locus_tag="VF547_09330"
     CDS             complement(14271..14648)
                     /gene="rplT"
                     /locus_tag="VF547_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX8263061.1"
                     /translation="MARVKRGVTTKARHKRILEQAKGYYGRRKNTIRIAKQAVEKAGQ
                     YAYRDRKVKKRSFRALWIQRINAAVRAEGLTYGQFMHGLKLAGVDLDRKVLADIAMHE
                     GEAFKGIVAQARGALPQESRATA"
     gene            complement(14662..14865)
                     /gene="rpmI"
                     /locus_tag="VF547_09335"
     CDS             complement(14662..14865)
                     /gene="rpmI"
                     /locus_tag="VF547_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX8263062.1"
                     /translation="MPKLKTKSGVKKRFKLTTTGKLKHGVAGKRHRLISHNAKYIRQN
                     RGTEVLAKADTARVKLWAPYGLN"
     gene            15044..16690
                     /locus_tag="VF547_09340"
     CDS             15044..16690
                     /locus_tag="VF547_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8263063.1"
                     /translation="MRWLAPLLILMASAGPAQAPRSPTPSGSPLIRIDPAAVKAHMAV
                     LADDLLEGREAGTRGDALAQLYIRTRFEEIGLAPAGDPGSYVQRFRVRSTRLVADSVD
                     FRIAGRAFANGGDVAIFGDPLETEQRVEADAVFAGYGIAAPERGIDDYRGLDVRGKVA
                     VVLGGPPPFLPPAEAAHHGWADQQRLVAESKGAVGLVILWTPALEQRFPFEGLKGLVG
                     RTDLNWIGRDGRPSVTAPGVRLRAFVRGEAAEALLARAPKTMAALLREAGKRSPPGFA
                     LASRVRLVRRSVHDDSLASANVAGLLAGSDPALSREVVVVTAHYDHVGIGEPERGDPI
                     YNGALDNAVGTAMLIEVARALAAAPGPRRRPILFLAVGAEEKGLIGSDYFAANPTVSE
                     PIADINLDGAMPFYDFRDVIAFGAEQSQMGERLAEAARELGLAVAPDPFPEEGIFTRS
                     DQYSFVRRGVPSLFLYMGFTAMDGRTVGRALWDELGRTVVHRPGDDLSQPIDYRVAAK
                     FADLFRRVTIATADAPARPLWYEHSLFRRFAAGRPAARRP"
BASE COUNT         2293 a         6071 c         5879 g         2515 t
ORIGIN      
        1 ccggtcgatc ccgcgaacgc cttcgcgggt gagcggaagg ctgctcgccg cgacgctggc
       61 cgggtcgccc gggtcgatgg cgagcacccc ctcccagccc cggcggaagc cggtcacctg
      121 ccacccgagc gcgtcggccg acagggtgat cgagcggatg caggggttga ggccgggcac
      181 gtcgccgccg ccggtcagca ggccgatccg catccgctca gcccgaggag gggaagcgga
      241 cccgaagctt ccggccgggg gcggtcggca cgtccatctg ggcgctgttg aaggtcgcgc
      301 tctggccggg ctggagatcc gagacgggcg gggaaatcga ccagctgtag accacggtct
      361 tgccgtcttg ctcgcggacc tcggcgcgga gctggggcac gcgctggacc gactgggtcg
      421 ggttgctgat ccgcccggtc acggtgagca gggcgttgcc gctcgccaac gtgttgttct
      481 gggccctcac gtcctcgacc tggagcgggg ttgcgctgct tcccggaagg gcgatgtcga
      541 cgcctagggc gggcaggtcg aacgagtaga gcgcggccgt cgccgcaagc atcagggccg
      601 ccgcgaagat ggcgatgatg gtccacattc tcgcccgatt gcgcctcggc cggaacggcg
      661 gctcgtgggc gaaggcgtca tattcctccg cggccggcgt cggcgcgggc cccagcatcg
      721 acgctgcggt ccggacgggc gcgggctcgc gcaacggcgg cggcgggctc tcggcagcgg
      781 agggcggcgg cttccattcg gccgccggcc gcgccgccag cggcggcggc ggctgcagcc
      841 agctgtgccg gcacgacgcg cagcgcacct gccggccgga cgggccgatc gcgctgtccg
      901 gcaccacata gcgcgtctta caggcgggac agctcaggat catcggctac tggaggcccc
      961 aaaggctggt gctcgcggcg aatccaagca cggtgaggcg ctccatgcaa gccattgcgc
     1021 ctctattgag tggtttacgg ccccgcgcgg cgcatgccat tgaagcgcaa gagcggaggt
     1081 cgatctgagc agcggcggca acatcgtgca attcgacaat gtcgggctgc gctacgggac
     1141 gggggcggag acgctttcgg acctgagctt ctcgttgcgc cagggcggct tctacttcct
     1201 caccggaccg tccggcgccg gcaagacgtc gctgctcaag ctcctctacc tcgcccagcg
     1261 gccgagccgc ggcctgatca agctgttcga cgaggacatc gtcaccatgc cgaggaagcg
     1321 gctgcccggc ttccggaggc ggatcggggt cgtcttccag gacttccggc tcgtccacca
     1381 tctctccgcc ttcgacaacg tcgctctgcc gctccgcgtc gccggagtcg acgagaagga
     1441 tcttcagggc ccggtcggcg agatgctcgc ctgggtcggc ctcgggcacc gcgccagcgc
     1501 ccgtccggcg accctgtcgg gcggagagca gcagcgcgtc gccatcgcca gggccgtcat
     1561 cgcccgtccg gagctgctgg tcgccgacga gccgaccggc aatgtcgatc cggaaatggc
     1621 caagcggctg ctgcatctgt tcgattcgct caacaagctg ggcaccaccg tcgtcgtcgc
     1681 gacccacgat ctccacctca tctcgagcat cggccggtcc gagatgctcc gcctcgaccg
     1741 cggcacgctg ctcgacccga ccggggctct tcgtcacccg ccgcggcggg ccgacgcgtg
     1801 agcaccgcgc cgcgaagctt cggcgccgcc gaccggcgcc tgctccccga agggcggctg
     1861 gccgggccga tgccctgggt gatcgcgatc atgatgttcc tgaccgtgct cgccgccgcg
     1921 gcggggctcg gcctcggcgg ggccgcggcc agcctgggcg ccggcctcgg caaccgggtg
     1981 acggtccagg tgctcgagcc caatccggac cggcgcgagg ccgaggcggc gcgggcgctc
     2041 gaccggctcc ggcgcctgcc ggaggtcgag agcgtccgcc ggctgtcgcc ggaggagatg
     2101 aaggacctgc tcgagccgtg gctcggcgcc ggcgggctcg agggcgacct gccgatcccg
     2161 gccatgatcg acgctcagct gatccccggc gcccgcgccg acacccttcg cggggcggtg
     2221 accgcggccg cgccctcggc ccggatcgac gacaatgatc aatggctggc gccgctcggc
     2281 cggctcatag ccgccctgaa atggctggcc ctcgggctgg ttgcgctgat gatcggggcg
     2341 accgccgcga ccgtcgtcct cgccgcccgg gccgcgctcg acacgcaccg cgggacgatc
     2401 gaggtgctcc acctgatggg cgccaccgac gtccaggtcg cgcgcctgtt ccagcgccgg
     2461 atcgccctcg acgccctgtt cggcggagtc gtcggcttcg ccgcggcggc gctcgccctg
     2521 ctcgtcatcg gccggcggat cggagcgctc ggctccgacc tgctcggctc ggccaacctt
     2581 ccggccacga gctggctggc gctggccgcc ctgcccgtcc tcggcgtcgg cctggccatg
     2641 ctggtcgccc gcgcgaccat cctgagggcc cttgggagaa tgttgtgatc cgtcgcgccc
     2701 tttccgccgt gctgctcgtc tacctgctcg gctatgccgt gttcgccgtg ctcctgcccc
     2761 ggccggccga cgagcggcgg accgacgcca tcgtcgtgct caccggcgga gcgaagcgga
     2821 tcgagcgcgg gctcgacctg atggagcgca ggctggcgaa gcggatgctg gtctcgggcg
     2881 tcgcccgaac cgtccggccg gaagagctgg cggcgcaata tcaggcggat tcggcgctgt
     2941 tcggctgctg cgtcgatctc ggccgcgaat cggtcgacac ccgctccaac gcggaggagg
     3001 tggcgcgctg gatggatcgc cacaagttcc gcaccatgcg cctcgtcacc accgactggc
     3061 acatgccccg ggcccgcttc gagctgtcgc gccggctcgg cggcggcatc gccgtgaccg
     3121 gcgacgccgt cgagagcaat cccagcttcg gccagctgtt caccgaatat aacaaatatc
     3181 tgctgcgccg ggcggccgtt ctcgtcggga tctgatgaac ctcgtccgct cgctgctcta
     3241 cgcgctcgtc ttctatccgg gcagcgtcct ctacgtcctc gccggcatcg ccatgatccc
     3301 gctcggccgc ggcgcggtcc gccgggtcgc cgacgcctgg gccggcttca accgcttgtg
     3361 cgcgcgcttc ctgctcggca tcaccagccg ggtcgagggc gaggtgccgc tcggcccggc
     3421 gctggtcgcg gtcaagcacc agtcgatgtt cgagaccctg gagatcgcgc tcctcctcga
     3481 cacgccggcg acggtgatga agagcgaatt gggccggatc ccgctgtggg ggcggctgac
     3541 ccgggtctac ggcatcctgc ccgtcgaccg cgacggcggc ggggcggcgc ttcgcaagct
     3601 gcttcgagcg gcggacgagg cgaaggcgga agggcggccg atcgtcatct tccccgaggg
     3661 cactcgggtc gcgcccggcg aatcgccgcc gctcgagccc ggcttcgccg gcctctaccg
     3721 ggcgctcggc ctgccggtgg tgccggtcgc gctcgacagc ggcaaggtct ggccgaggcg
     3781 ctccttcgtg aagcgccccg gggtggtgac gatccgcttc ggcgatccga tcccgcccgg
     3841 cctcccccgc agggaagcgg aggcccgagt ccacgccgcg atcaacgccc tcgaatgatc
     3901 ccagccactt gaccctcggt gcctggcacc gaacccgccc cgccgcgggg ttcggctccc
     3961 aatgttctca gctacttatc cctcggtgcc tggcacctaa ttcgagccgc cgcggggttc
     4021 gggtcccaac gatctcagtg acttagcgct cggtgccagg caccaaattc gagccgctgc
     4081 ggggttcggg tcccaatgat ctcagccact tagcgctcgg tgcctggcac caaattcgag
     4141 ccgctgcggg gttcgggacc taacgatctc agtcacttag cgctcggtgc ctggcaccgg
     4201 attcgcgcgg attcgcggcg ggtgggcatg gcggcggggg caggtgaagc tgtcgcgcgg
     4261 agggcggcgg cgggccggct caatcgtcgc catcgagcca gtggacgagg ctgccgtgcg
     4321 ggtgggcgta ggcgagcagg ctctcctggc cgggccaggt ccgcagcctc agggtcagcc
     4381 gctcgtccct cgggtcgtgc tcgtagcgca gccaggccag cggccggccc gccgccgccg
     4441 ccgccccgga gcgcgccatc gccgccgcga tccgcgcctt cgacgcctcg tcgaaatatt
     4501 ggaaggcgat cgagtggaac acgacccggg tcacgcccgc cgccgccggc tcggccagcc
     4561 tcgactcgag ccagccggcg gcgtcgcccc gctccaccgg cgccggctcc gcgcgcgcga
     4621 tcgccagcgc cgcctcgagc tgggccagcc gccgcctctg gtcgggccag acataggcga
     4681 tgagccgctc gccgtcgcgg cgcgggtcga gcggcctcag gtcgactccg cggcggcccg
     4741 ccacctcgac ccgagccgcc ggcggccggt cccccatcca gtcgggcttc agctgcaacg
     4801 gcgaggccgg atcgccggcc cgcaatccgc cgaggtcgta gccgtagcgg tcgagcagca
     4861 ggttgaggcc ggcgctggcg ccgagctcga gaagctcgac cggctgcggg aaaaggtcgg
     4921 cgacgacgag cagccccgcc atcagcaccg ccgagcgccc gacctcgttg gtctgcggcg
     4981 ggccgtcgag ccacggcatc agctcggggt cgccgagcac cggccggacc gccgcccaca
     5041 gggcctcctc gtcgggcagc gggcgcggcg gatagcaggc ggccagcccc ggcgcccggc
     5101 cccgccgcac cagcgcatgg agcccgccgg tcagccgaag cggcaaggcg tcggccagcg
     5161 aatcgggctc gcccggccag tccagcaccc gccggccgag ctcggtcttc cggtcgagcc
     5221 gctcgccgag cagccggcaa agccggacca tgaagggcga tccgagcgcc tccgcccaat
     5281 gcgcctggcg ggcgaaggcc gcccggacgg cctcctcagt cgcggagccg ggcaccggcc
     5341 ttggccgcgg cggcgacgat ccggccggag accgccttca gatcctcctc ggtgaagctc
     5401 ttctcccccg gctgcagcac cacctcgacg gcgagcgact tgagcccctc cggcacgccc
     5461 ggcccggtga agacgtcgaa cagcgagacg gcggcgatcg cctgccggtc ggcgccgcgc
     5521 accgcccgaa gcagggtttc ggcgggaacc tcggccgcga ccaggaaggc gaagtcgcgc
     5581 ttcaccgcct gcaaggcggg cggcgcatag gcctcgcgca tccgtccccc tccgccgcgc
     5641 cgggccggaa tggcgtcgag atagatttcc gcggcgaccg ccggcccgtc gaggtcgaac
     5701 gccttgagag tcgcgggatg gagctcgcca tattcggcca gcacggtctt cggcccgagc
     5761 gcgagccgcg ccgagcggcc gggatggtag acgggccccg cgcccgccag cgactggagg
     5821 ctgtcggccg gagcgccggc cgcggcgagg atggcgagcg cttccgcctt ggcgtcatag
     5881 gcgtcgaagt cctgggcctt gccggtccgc cagtggcgcg gcgcccgctc gccggcgagg
     5941 acgaggccga gcgtcggccg ctcgccgtcg gcgaggtagc gccgcccgag ctcgaacagc
     6001 cgcaccccgc tctggccgcg gtcggcgttg cgccgcgccg cggcgagaag gccggggagg
     6061 agcgagggcc gcatcgcctt catctcctcg ctgatcggat tggcgaggat ccaggacgat
     6121 ccgccgaagg cgtcggcctc gctctcgctg acgaagctcc aggtcaccgc ctcgttgagc
     6181 ccgcgcgcgg cggcggtgcg ccgcgcccgc cgctcgatca gctggtcggg ggtcgcggtc
     6241 ggccgcgcca ccccggccgc ccgcggcagc acagtcgcct cgaccttgtc gaagccctcg
     6301 atccgcgcga cctcctcgac gatgtcggcg gcgccgtcta tgtcccgccg ccacgagggg
     6361 acgatgatcg tccactgctc accctccagg acggtgaagc caagccgctc gagtatttcc
     6421 ttctggcgcg gctccggaac atcgagtcca cccagctcgc gagccctggc aggccggtat
     6481 tgaatggatt tctcaagctt cggcggctcg ccggcgcgga cgatctccga aggctcgccc
     6541 ccggccagct cgatcgccag ctcggtcgcg atgtcgagcc ccggcccgac gaattcgggg
     6601 tcgacgcccc gctcgaagcg ggtacgggcg tcggacgcga ggccgagctt ctggccggtg
     6661 cgggcgatgc tcttcggatc gaaataggcg cattcgatca ggatgtcgga cgtcgcctcc
     6721 gtcaccccgc tatgctcgcc gcccatgatt ccgccgatgt cgtggacccc gcgcgcgtcg
     6781 gcgatcaccg tcatcgacgg atcgagccgg taggacttgc cgttgagggc ggtcatctcc
     6841 tcgccgtccc gcgcccggcg ggcgacgacc gcgccctcga gcttcgccag gtcgtagacg
     6901 tggagcggcc ggccgtaggt gatggcgaca tagttggtca tgtcgaccag cgcgctgatc
     6961 ggccgctgcc cgaccgcctt cagccgccgc tgcagccagt ccggcgacgg cccgttgcgg
     7021 acgccgcgga cgacccggcc gtaaaaggcg gggcaggcct cggggtcctc gatccggatc
     7081 tcgaccgggc aggggaagct gccctcgatc tcccttgtcc ggaccggctt caaggccccg
     7141 agcccggccg cggcgaggtc gcgggcgatg ccgtggacgc ccatgcagtc ctggcggttg
     7201 ggggtgacgt tgacctcgat cacgggatcg tcgagcccgg cccattgcgc atagcccgcg
     7261 ccgaccgggg cgtcggccgg cagctcgatg atcccgtcat gctcgtcgcc cagctcgagc
     7321 tcgcggaccg agcacatcat gccgcggctc tcgactccgc ggatcgtcgc gatcttcagg
     7381 gtgaggtcgc tcccgggcac gtaggcgccc ggcgctccga acaccccgag catccccgcc
     7441 cgggcgttgg gcgctccgca cacgacctga accgcctcgc cggtgcccgc gtcgacggtg
     7501 agcacctgga gcttgtcggc ctgggggtgg cgctcggcgg tgaggatcct ggcgacggtg
     7561 aacggcgcca gcgtctccgc cgcattctcg acgccctcga cctcgaggcc gatcgcggtc
     7621 agcgtgccga cgatcgtctg caggtccgca tcggtgtcga gatgctcctt cagccaggaa
     7681 agcgtgaact tcatcctgct cccctccctg cacgcaggga ggggtcggga gcgggtgcgt
     7741 ctccggacct gcaccgccct ccattggcgt acccacccct gccccctccc tgcgtgcagg
     7801 gagggggata gatcgtcgtc atgcccccac gcctccgctc agagtcggca cgtcgagggc
     7861 ggcgaagccg taatgcttca gccacctgag gtcgccgtcg aagaaggcgc gaaggtcgtc
     7921 catgccgtat ttgagcatcg ccagccggtc gaccccgcag ccgaaggcga agccctgcca
     7981 ttcgtccgga tcgagcccgc aggcggcgat cacccgggga tggaccattc cggagccgag
     8041 cagctccatc cacccctcgc tcccgcccag cacgcgccgg ccgttcaccc gggtgaagcc
     8101 gacgtcgacc tcggccgaag gctcggtgaa cgggaaatag gagggacgca gcctcagcac
     8161 gatgtcctcg cgctcgaaga aggccttgag gaaggtctcc agcgtccatt tcaggtggcc
     8221 gagatggatg cccttgtcga tcaccagccc ctcgatctgg tggaacatcg gcgtgtgggt
     8281 ggcgtcgttg tcggaccggt aggtccgtcc cggcgcgatg atgtagatcg gcggcgcctg
     8341 cgactgcatc gtgcggacct ggaccggcga ggtgtgggtc ctcaggagca tcggctcctc
     8401 cgcgccgtcc ccgccttcgc cgcgctgccg caggtagaag gtgtcgtgca tcgcccgcgc
     8461 cggatggtcc ggcgggaagt tgagcgcggt gaaattgtgc cagtcgtcct cgatctccgg
     8521 cccggtcgcg accgagaagc cgaggtcggc gaagatctcg gcgagctcgt cgagcacctg
     8581 gctgaccgga tggacgctgc cctgcgccgg cggcgccacc ggcagcgtca ggtcgagccc
     8641 ctcggtcgcc agccgccgct ccagcgcctc ggcctcgagc gccgccttgc gcccggcgat
     8701 cgcctccgcg acccgctcgc gcaggccgtg gatccgcggc ccctcggcct gccgctcctc
     8761 tggcgtcatt gcgccgagcg tcttgagaag ctgggtcacc gccccctgct tgccgagcgc
     8821 cgcgacccgc tccgcctcca gcccggcgag gtcggcggcg gacgagatgc gggcgagaag
     8881 atcgccggaa agtcggtcga gatcggccat ggcgcgggct ctagagaata tttcccggcc
     8941 tttgaaccca cccccgtcgt catcctgaac ttgtttcagg atgcagagcg gaccgctcga
     9001 cggccgcctg ccgccctcgc atcgcccgcc tcagctccca ggccgcgggc caatcagtct
     9061 cgaatggaag gcgcaaaggc acgaaggaga ggttcgagac cgtcccctcc tccgcttcgc
     9121 gccttcgtgc cttcgtgcct tctcttgaaa ccggcgcccg cggcgccggc ggaagggcac
     9181 aagcgctcta cccatatcca acgtccgagc cagccgatcc cgacccctca aacaagttca
     9241 gggtgacggg cggtgcggat gggggcacat tcccccgatg gcttggcccg aactcagcga
     9301 agcccgcgac gggccgacga tcgccgccct ccatttgtac agccaggtcc tcggcaaggc
     9361 cgcggtcgcg cttctgccgt ggcgcaacca tggctggcac gtcacgctcc gggtccatcc
     9421 gcgcgggctg aggaccgagc cgctctacgg cgccggcggc ccgttcgagc tgggactgga
     9481 tctggccggc caccaattca ccttcgccga cggcggcggc cttcgccggt tcgagctcgc
     9541 tccggtgccc gtgtcgcgct tccacgccga ggtgatgacc tgccttgccg cggccggaca
     9601 cgaggtccga atccacccgg ccaccaacga gctcgacccg ccggtccgct tcgccgacga
     9661 ccatgtcccc cgcgcctatg accgcggcag cgcctcgcgc ctgctcggcg cgctgctcga
     9721 ctccgaccgg gtcttccgcc ggttccgctc gggcttcctc ggcaaggcga gcccggtcca
     9781 tttcttctgg ggcagcttcg acctcgccgt cacgcgcttc tcgggccgcc cggcgcccct
     9841 ccaccccggc gggatcccct atctgcccga cgagatcacc cgcgaggcct acagccacga
     9901 agtctccagc gccggcttct ggcccggcgg ggcgggcggc ggcggcgatc ccttcttcta
     9961 ttcctacgcc tatccctcac ccgagggatt cgcggcggcg ccggtgcggc cggaggcggc
    10021 gcgcttcgac gaggcgctgg gcgagttcat cctcccctac gaggcggtgc gcgcggcggg
    10081 cgatcccgac cgcgccctgc tctccttcct cgactccacc tacgccgccg ctgccgaccg
    10141 ggcgaagtgg gaccgcccgt cgctggaatg cgagccgggc cggcccaggg tgccgcggcc
    10201 gctctgatcc tcccggagat ttcccgcgga gggcaaaatc tcctccgtcg ccccgcttcc
    10261 cgttccggct gagcgtgtcg aagccctctc cctttttctt acggaacgac gaggaaagcc
    10321 cttcgacagg ctcagggcga acgggtgaca gcgattcagc tagtgcgact tgagcccggc
    10381 cgcctcgagc gcgttcatct gaagctcggc gcatttcatc gcctcggtca ggaagccctc
    10441 gtcggcggcg atgatctcct gggcccggtt cagcccctcg agccaggcct ggagcggata
    10501 ggcctccatc agcccggcca tcgccgcttg gtcctccgcg ctgggggtcg cggcggcgtt
    10561 catccgggcg cccagcgcct cgaaggcggc gtaatcgggc cccgcgtaga aatcggccag
    10621 ccgcttcact ttggcttccc cgaggtcgcc ggcgacggtc cggagcatgc gccgggtgcc
    10681 cgccgagatc gcctcgttcg agcaggcgag gttgccctcc aggaccgagc gcacctgggc
    10741 ctccttgccg ggattgagcg cggcgagggc gccgagctcg gccgggtcca cttcggcgct
    10801 gggcgagtcg atctccccgc ggtcgggcag gacggcgagg aagcggtcga tcgtcgcctc
    10861 gctcgccggc gccttggcgg cccgggccgc cagcggcggc gcctggagga cgagcagggc
    10921 ggcggcggcg gcggcgaatg gtctcatggc gtcatgtcct cgaagcttgc gcggctccgc
    10981 gcaggaggac gcttggagcc tgcgatcatg ttggaaccgc ccggccttgt ccagcgatcg
    11041 cgacaagagt ccggagcgct cagcccacga acggataagg cgagatcgtc ttctgggtct
    11101 cgttgagggc gagggtgcgg ccggcggggg cggcggcacg ctgggggcac ctgaggcgcg
    11161 ggcagatggt gcaggcgggg ccgatcgggg tgaccagcgg cttggccagg tcgtggccgt
    11221 cggcatagac gatcctcggc gcatatcggg cgtcgcagcc gaggccgatg gcgagctgcg
    11281 agctctcgcg cgggtcgagg cggacggcgc ggtcgacggt ccgggcgagg gtgaagaagc
    11341 gcgccccgtc gggcgtctcg acgatctggg tggcgacccg gccgggggtc tggaaggcgg
    11401 cgtggaggtt ccagcgcggg caggtgccgc cgaaacgcga gaaggggaag gactcgccgg
    11461 cgaaccgctt ggagatgttg ccggccgcat ccacccggag catgaagaag ggcacgccgc
    11521 gggcgccggt gcgcgccagc gtggtgagcc ggtgcgagat ctgctcgacg ctggcgccga
    11581 aatcggcgca cagccggtcg acgtcgtgcc gatgcttctc ggccgccgcg aggaagccgg
    11641 catagggcat catgatcgcg ccggcggcat aattggcgag gctcatgtgg agcagccgcc
    11701 gggtgggagc gtccggcgcg ccggcggttt cgaccatccg cttcagcatc ggcccgaact
    11761 cgaccagggc gagctggtag gcgaccgcga aggtccggct ctccggccgc agcatggcgg
    11821 agagcatcag cctcttgcgg tggtgatcat aggcctggct ggtcggctcg atcagctcgg
    11881 gcggcaccac ccgggtgtcg atcccgaagc cctcgcggag ccgccgccgg agcgggtcga
    11941 agatcccgtt cggatcgccc agcgccccgg ccagggtctc cgccgcctcc tcgatatagg
    12001 ggaaatggtt gcgctgcgcc tggatgtggt cgcgcaccca ggcctcgggg gtcaccggcg
    12061 aggtctcgtc cgggaccgcc cccgccggat gctgccggcg ctcgaccagg gcggcgtaga
    12121 gcctcgagat ggcgtcggcg acggacggcg cattgtcggc gacctcgagc agctcgaagc
    12181 gcgggatgcc gatgtcggcg aacatcgggt cggcgaagat ctcggcgagc tggtcgatcc
    12241 cggtcccgtc cgccccttcg gcggcgaagg cgcgaagatc gacgtcatag acctccgcga
    12301 gccggagcag cacctgggcc gtcaccggcc gctggttgcg ctcgagatgg ttgaggtagg
    12361 agggggagac tccgatctcg cccgccatcg cgctctggtt gaggccgagc tcgcggcgca
    12421 gccggcgcag gcggccgccg agatagagct tgcgttcggg agcgggcatc ggtcgaatat
    12481 ttgtcacagc tttacaactg tcgcaagtcg cgttgtgaca gcgcgaccct tttcggcgcg
    12541 cccgcggctc ggcgatgcgc tttcatagtc tggcgatctc accaacttcc gaggcgaaaa
    12601 tgagcgactt cacgaccatc gtcccggccc cgccccggcg cttcgacggc atcgaacggc
    12661 cttattcccc ggaggacgtg ctcaggcttc gaggctcggt cccggtcgag caaagcctcg
    12721 cccgccgcgg cgccaaccgg ctctgggagc tgcttgcgcg gcacgatcat gtccattccc
    12781 tcggcgcggt caccggcaac caggccatgc agatggtccg cgcgggcctg aaggcgatct
    12841 acctttcggg ctggcaggtc gccgccgacg ccaacaccgc cggcgccatg tatcccgacc
    12901 agtcgctcta cccggccaat gccggcccgg aattatgccg cagaatcaac aggacgctcc
    12961 agcgcgccga ccagatcgag catgccgagg gcggcgctcg gcgcgactgg ctcgtcccga
    13021 tcgtcgccga cgccgaggcc ggcttcggag gccccctgaa ctgcttcgag atcatgaagg
    13081 cctatatcga ggccggggcc gccgcggtcc atttcgagga ccagctcgcc agcgagaaga
    13141 agtgcggcca tctcggcggc aaggtgctga tccccacccg ggcccacatc cgcaacctcg
    13201 tctccgcccg cctcgccgcc gacgtctgcg gagtccccac cctggtcgtc gcccgcaccg
    13261 acgccgagag cgcgaggctg atcacctccg acgtcgacga gcgcgaccgc gagtggctca
    13321 gcggcgagcg caccgccgaa ggcttcttcc gcctccgaga agggaccggc atcgaccatt
    13381 gcatcaagcg cggcctcgcc ttcgcgccct atgccgacct gctctggtgg gagacgagca
    13441 atcccaatct cgacgacgcg cgccgcttcg ccgaggcgat ccaccgcgaa tatcccggca
    13501 agctgctggc ctataactgc tcgccgagct tcaactgggc ggcgaagctg gacaagggca
    13561 cgatcgccgc gttccagcgc gaactgggcg cgatgggcta caagttccag ttcgtcacgc
    13621 tcgccggctt ccacagcctc aaccacggga tgttcgagct cgccagcgcc tatcgcgacc
    13681 gcggcatggc cgcctattcc gagctccagc aggccgagtt cgcctcggaa agcctcggct
    13741 acaccgccac ccgccaccag cgcgaggtcg gcaccggcta tttcgacctg atcgcgcaga
    13801 cggtcgccgg cggcgaggcc tcgaccgtcg cgctcgccga atcgaccgag gccgagcagt
    13861 tcatcgccgc cgtcgcggcg gaatagggag acccgacatg accaagcgct tctgggtggt
    13921 cgggggcgaa tatgagggcc cggacttccg cgccctgatc ccgggcacgg aaaccatggc
    13981 cggcccgttc gaggacgagc ggagggcgag gaccgagtgg acccggctga gctgctgccc
    14041 ggagaagagc ggccgggcga cggtccgcta ttcgatcgcc gcggagacct tgcactgatc
    14101 catcccctct ccccttgggg agagggtcag ggtgaggggg ttcggcgtct tcgcgaaagg
    14161 cgcacagacc ccctcacccc cggcccctct ccccgtcggg gagaggggag caaaggaaaa
    14221 ggcgcggcag ggtctccccc gccgcgcctt cctttaatct cagccgaagc tcaggcggtc
    14281 gcccggctct cctgcggaag cgctcctcgg gcctgggcga cgatgccctt gaaggcctcg
    14341 ccctcgtgca tcgcgatgtc ggccaggacc ttgcggtcga ggtcgactcc ggcgagcttg
    14401 aggccgtgca tgaactggcc gtaggtcagc ccctcggcgc ggaccgcggc gttgatgcgc
    14461 tggatccaga gcgcccggaa cgagcgcttc ttcaccttgc ggtcgcgata ggcgtattgg
    14521 ccggccttct cgacggcctg cttggcgatc cggatcgtgt tcttgcggcg gccgtaatag
    14581 cccttcgcct gctcgaggat ccgcttgtga cgagccttgg tcgtcacgcc ccttttgacg
    14641 cgtgccatgt cccaatacct cctagttcag gccgtagggc gcccagagct tcacccgggc
    14701 cgtgtcggcc ttggcgagga cttcggtgcc gcggttctgg cggatgtact tggcgttgtg
    14761 gctgatcagc cggtggcgct tgccggcgac gccgtgcttc agcttgccgg tggtggtgag
    14821 cttgaagcgc ttcttgacgc cgctcttggt cttcagtttg ggcattttcg tctcctgtgg
    14881 atccccgctt gcgcggggac gggcgtttga gaacggccac ggcagcccta tcggccgggc
    14941 cgttcgtttc ttccaaagct ggaaagaagg cgcgcctata gagggggaag ccgggaaagg
    15001 caagcattgc ggcgccgccc ggccgcatgc caagcagggg ccgatgcggt ggctcgcgcc
    15061 tctcctgatc ctcatggcct cggcgggccc cgcccaggcg ccgcgctcgc cgacgccgag
    15121 cggaagcccc ctcattcgca tcgatccggc cgcggtcaag gcccatatgg cggtgctcgc
    15181 ggacgatctg ctcgagggcc gggaggccgg cacgcgcggc gacgcgctcg cccaactcta
    15241 tatccggacc cggttcgagg agatcgggct ggcgccggcc ggcgatcccg gcagctatgt
    15301 ccagcgcttc cgggtgcgct cgacccggct ggtcgccgac agcgtcgatt tccggatcgc
    15361 cgggcgcgcc ttcgccaatg gcggcgacgt tgcgatcttc ggcgatccgc tcgagaccga
    15421 gcagcgggtc gaggccgacg ccgtgttcgc gggctacggg atcgccgctc cggagcgcgg
    15481 gatcgacgat tatcgcgggc tcgacgtccg cggcaaggtc gcggtggtgc tgggcgggcc
    15541 gccgcccttc ctgccgccgg cggaggcggc gcatcacggc tgggccgacc agcagcgcct
    15601 cgtcgccgag tcgaagggcg ccgtcgggct cgtcatcctg tggactccgg cgctggagca
    15661 gcgcttcccc ttcgagggcc tcaagggcct ggtcggccgg accgacctca actggatcgg
    15721 ccgggacggc cgccccagcg tgacggcgcc cggagtgcgg ctccgcgcct tcgtccgggg
    15781 cgaggccgcg gaggcgcttc tggcccgggc tcccaagacg atggcggcct tgctgaggga
    15841 ggccgggaag cgctcgccgc ccggcttcgc gctggcttct cgagtccgcc tcgtccggcg
    15901 ctcggtccat gacgacagcc tggccagcgc gaacgtcgcc ggcctgctcg cggggagcga
    15961 tccggcgctg agccgcgagg tcgtcgtcgt caccgcccat tacgaccatg tcggaatcgg
    16021 cgagcccgag cgcggcgatc cgatctacaa cggggcgctc gacaatgcgg tcggcaccgc
    16081 gatgctgatc gaggttgccc gcgccctcgc cgcggcgccc gggccgcggc ggcggccgat
    16141 cctcttcctc gccgtcggag cggaggagaa gggcctgatc ggctccgact atttcgccgc
    16201 caacccgacg gtgagcgagc cgatcgccga catcaacctc gacggcgcca tgcccttcta
    16261 cgacttccgc gacgtcatcg ccttcggcgc cgagcagtcg cagatgggcg agcgtctcgc
    16321 cgaagcggcc agggagctcg gcctggcggt cgcgcccgat ccctttccgg aggaaggcat
    16381 cttcacgcgc tccgaccagt acagcttcgt ccggcgcggg gtcccgtcgc tcttcctcta
    16441 catgggcttc accgcgatgg acgggcggac ggtcggccgg gcgttgtggg acgagctcgg
    16501 aagaacggtg gtccaccggc ccggcgacga cctgtcgcaa ccgatcgact atcgggtcgc
    16561 cgccaagttc gccgacctgt tccgacgggt gacgatcgcg acggccgacg ccccggcccg
    16621 cccgctctgg tacgagcaca gcctcttccg ccggttcgcg gccggccggc cggcggcacg
    16681 gcggccctag cgcccgcggc cggcctcccg gtcacaattg aaacacttcg ggccgccgcg
    16741 cggcatccat ctgcaagg
//
LOCUS       DASVQZ010000332        16897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_266923, whole genome shotgun sequence.
ACCESSION   DASVQZ010000332 DASVQZ010000000
VERSION     DASVQZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 16897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16897
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266923"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VF547_09345"
     CDS             <1..820
                     /locus_tag="VF547_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX8263064.1"
                     /translation="ANVGDRIDAVVSSIGDARSLAGGTLLMTPLMGPDQRPYALAQGS
                     IITGGHSFESELNQNQRNYPTSAVLQGGATVERPVDAQIMRPGGELAFLLNDPNFGTA
                     QRIANGINSRFGAGSATVRNADEVRIRFAGDPTQFAAYVAELESLSVQPERPPRVVIN
                     ERTGTVVAGGDVMISSVVISQGDVKVTVTADNYASQPDFYSGFASDVRSLVVTNTKLD
                     VEQGAGDAVVSFPNTSVADLVQGLYRARVNTRRTISILQAMKAAGALHAELIVQ"
     gene            834..1178
                     /locus_tag="VF547_09350"
     CDS             834..1178
                     /locus_tag="VF547_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263065.1"
                     /translation="MDPTSAIMITKALDGLWARSLATAQNVANANSPGYRPVRVSFEE
                     ALQAASRQGPGALSGVRATVGQAPAGTKGAELRLDLELATASETGLRYAALLDLLGRQ
                     LQISHTVIRGGQ"
     gene            1180..1608
                     /gene="flgC"
                     /locus_tag="VF547_09355"
     CDS             1180..1608
                     /gene="flgC"
                     /locus_tag="VF547_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX8263066.1"
                     /translation="MRAMEISRSGLDVEWQRLQVIAQNLANLNTTRTATGEPFRAMRL
                     LSGPRADFAQMLSGKGSAQQLRGVQVMGMEANGAGFRRVHEPKHPHADADGFVSYPDI
                     DHAGEMTLMIKTSRTYEANLTAMNIAQQMYSRALELGRGQ"
     gene            1605..1928
                     /gene="fliE"
                     /locus_tag="VF547_09360"
     CDS             1605..1928
                     /gene="fliE"
                     /locus_tag="VF547_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX8263067.1"
                     /translation="MTGIEAIGAIGLGTGDAAISRLSLTAPVAPVQTNQVGFSDVLAS
                     GMRHVEGKLATANELVQRFALDDSVPLHQVTYALEEARMSVELAMQVRTRLVESYREL
                     MNMQI"
     gene            1942..3207
                     /gene="fliF"
                     /locus_tag="VF547_09365"
     CDS             1942..3207
                     /gene="fliF"
                     /locus_tag="VF547_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX8263068.1"
                     /translation="METITLVPAGPSRQIKMLSLVFAALFVLLALGYYFFIRTEYAVL
                     YSGLRPADASAIVAELDAKGVAYQLKDQGTTIMVPKGESDSVRLAIVRSDAPLKGSSG
                     FELFNKSDMGLTDFAQKINYQRALQGELARTIMMMDGIENARVHLTIPERTLFRGNRS
                     EPKAAVTLTLQRGKAVDEARVAGIQRLVAAAVPDLKLADVVVLDEVGRIISSNAEAQA
                     DLPPELAERNAVQEYYRAHIRSAVEAVLPGLGADARVLIVPTASAADTGWEPSGAPAE
                     AVKLQASSRDPSVRNFQLRISVITPAPLNLEDQDRVRAALTRAVGLKPESGDSLQFGL
                     KVAPARAGVPIAPPVAAAGTVDYRKAAGIPQGNSTWLIALAALVALLLAAIAVRSRSS
                     GISTEQRDLLVLRIRRQLSLIDGTSDARG"
     gene            3194..3586
                     /locus_tag="VF547_09370"
     CDS             3194..3586
                     /locus_tag="VF547_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263069.1"
                     /translation="MREADTLTGLAEELRALTARERRGVLAALSPFERAQVGALIEAA
                     ATPEPAEPPAPDYSRFSPWLARHLGADETGGADRNLTAATRRLLAEARAELMSGPRAS
                     SRAPEPAPKRRSLADAVAQLLSPRQAHR"
     gene            3583..4239
                     /locus_tag="VF547_09375"
     CDS             3583..4239
                     /locus_tag="VF547_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX8263070.1"
                     /translation="MSGLIKGGAAHSVRPFLDPAELPPAPGGDPRLEALERENDELRA
                     AIAAQRKAGEEAVAAARAEGERQGRAAADDAADKRLQLVRKGVDAAIAVWEKRLADLD
                     DLAPSLARAALAKLFDDNEDHSRFVAGLIGRQLRLLRRDSVVAIRVSVRDFPDEQALS
                     QLGAEAATGSIRILADPDLASGGCRVDLQLGHIDIGTKTQWPQLAAFLDKLAEPEAGE
                     "
     gene            4236..5558
                     /locus_tag="VF547_09380"
     CDS             4236..5558
                     /locus_tag="VF547_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEX8263071.1"
                     /translation="MTAHRFLAKLDRADFARPYGYVRRVAPGLVEASGPAGRLGELCE
                     IECEDEPALLAEIAAVDERRVVLIPLEQQATIRPDARVTARPMRDRVAVGDGFAGRAV
                     DALGRPIDGLGPIAADAHAQLAGSVPKPMHRADPSRVLPTGIRAIDGLLTLGQGQRIG
                     IFAASGVGKTSLMEQLATQVDCDRCVLCLVGERGREVEAIWRLLSGREDSERFTIVAA
                     TSDESAALRGRSVSYALALAEHWRAAGDHVLLMVDSVTRLAMALREVGLAAGAPPTVR
                     AYTPNVFSALPRLVERCGAVRSGGAITAIMTVLSETDDVDDPIVELMKSLLDGHIVLS
                     RSLAEQGHFPAIDVPRSVSRGAQQLVVSEQASAMREAVAQLAAYEEARVMIESGVYKS
                     GTNGAVDRAIAKRGEMNAFLRQGRTENSAFPETLRKLRTVTAGASSHA"
     gene            5551..6000
                     /locus_tag="VF547_09385"
     CDS             5551..6000
                     /locus_tag="VF547_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263072.1"
                     /translation="MRDRVKLPLMVRLASVQRVKKAAAEAALMSARSAEAAARTAEEE
                     AQARTQAAQAEWGDHISGSAFSPEFSRALAARVIEGEKAASSASLRRERAAEITVRRK
                     DDWQASEARSRSGDESLRQLRRRVRRRLEEDRLAEMSDRVTFDWSRR"
     gene            5997..6833
                     /locus_tag="VF547_09390"
     CDS             5997..6833
                     /locus_tag="VF547_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263073.1"
                     /translation="MSAGPVTSRPPVDPGLAQRQPGEAASESGEGGASFADLLAAMLA
                     PGAAPLAEPGTAAPGTPEAVFDRLDSAEIFNEAGLFRGAAPLAAASLDPGLGRLPGTP
                     SPSLPSDAALEVEPSALAGPGPVPADAEPGPASSSARFVLDAALTGSPGAGPAASAAG
                     AAGSAPAPRGHPAPAPMASSQAPETAPAGSADPVRASSRRAVMLVQAHLAKAASTSAQ
                     VSAQAVEAGISLVVRADKLGREERDRLRVEIAELLLSHGFAMAEMTLNGEAWPVPHRK
                     DD"
     gene            6833..7273
                     /locus_tag="VF547_09395"
     CDS             6833..7273
                     /locus_tag="VF547_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX8263074.1"
                     /translation="MQTGSVSATQPGGAAVQPPRSAQDTATNAFGLSFESLLKIILTQ
                     LTYQDPLKPMDNFQFVSQLAQFSQIQLGQTTNDRLGSMVTAQANLQAAGLLGRTVDIP
                     AGAATISGKVMSIAFQNGDPRLTIETADKQTISNISIASISQIR"
     gene            7287..8465
                     /locus_tag="VF547_09400"
     CDS             7287..8465
                     /locus_tag="VF547_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX8263075.1"
                     /translation="MFGSIYVGLSGLNAYSRGLQQISNNVTNLNASGFKGSTVAFTDL
                     VGINGNGGASYSGGGGGGGNGVEIGDTRIDFKQGELRQTDRDLDLAIDGSGFLLLMRG
                     EEVLYTRTGSFEVDKDGFIVLAGTEYRLATLDGSGRPVSLSIDASRTSSPKPTTTIKF
                     ADNLSSTATTYGIADVKVYDANGGEHVWQIKFDKAEGALNEWKITITDDKAKTVGTKT
                     LKFVQGQVDPTTNKLLFEDTEGGLSVTLDFSQNVTSFSAGTVSTLRKASSDGYKVGAI
                     TSISVNDKGKLEIGYSNEQKKELGSIAIADFRDPQALEQRSGGLFRDTGLAQRELTSS
                     DDKRVGRVLSRRLEASNVDLAKAFGELILIQRGFQASSQIISVSNDMIQQLFGIRGQG
                     "
     gene            8499..9293
                     /locus_tag="VF547_09405"
     CDS             8499..9293
                     /locus_tag="VF547_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX8263076.1"
                     /translation="MPFRTWLPSGALGGGAVDRLLAETTGQWSDQWFARRLMRAVGET
                     ASLDRAELGRMRDLQLRYVEGGLALALEDDAATVIARMMLDESPDRRAVTEADEELVE
                     RLATGCIDDLCNRLAGAFGLGRTQSWRRGGIDGLPFAEACVFALGPSDNAPTIRVLVS
                     HELVAGIVRSSAKPARKALPLRPLAEALAKQSVDVSAVVGRCELSLGELAGLAPGDVL
                     VLDASTGGTLPLAINGDARSGRCAIEQEDGQLRLKIVKPLTGSARD"
     gene            9286..9585
                     /locus_tag="VF547_09410"
     CDS             9286..9585
                     /locus_tag="VF547_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HEX8263077.1"
                     /translation="MTEPASKTPPTATAPKADSALGNINPKLIETVSVTLEAYLGEAR
                     MTVADLTALKDNSVVPLDAPLNQAVELRLNGVAVARGELVAVGDSFAVRLTEIAK"
     gene            9582..9989
                     /locus_tag="VF547_09415"
     CDS             9582..9989
                     /locus_tag="VF547_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263078.1"
                     /translation="MIRPGPMATLCAGAAFLAASPALAGGGRLGGGQPLEVSLGRIVS
                     ALVICIIIAVLAVLLIRQRSGKIDLHALFGRFQVRPRAIEVVETRRLSPHADICVVRN
                     AGREYLLLLLAGNARVLREEPVVALPDAGGDAL"
     gene            9986..10702
                     /gene="fliP"
                     /locus_tag="VF547_09420"
     CDS             9986..10702
                     /gene="fliP"
                     /locus_tag="VF547_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545487.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX8263079.1"
                     /translation="MSLRRLAVRLGAALAVLAAAEPALAQATAGVGPDVARTALILTL
                     LAVVPAILVSMTSFIRIVIVLAMVRHAFGMPETPPNAVLVSLSLFLTAFTMSPVLSEL
                     NRDTVQPLLAGKIGVERAMETGSAPLRKFMLTQTRDADIESIYAISNTPLPERPEQVE
                     ILKLAPAFILNELRVAFTIGFVVLLPFLLIDLVVSSILLSLGMMMVPPTTISLPIKLL
                     MFVLIDGWKLVLEGVLGSFG"
     gene            10699..11436
                     /locus_tag="VF547_09425"
     CDS             10699..11436
                     /locus_tag="VF547_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8263080.1"
                     /translation="MSRAALPTGLALVVHPTRAEASLWRRLRFEAETECRETLFNRYV
                     PLARAIAAAKFHQRRGRAAERSDCEHFAYEGLLQAIDRFDPLLGVPFSAYARRRIVGS
                     ITDGLAKMSEVNAQISHRHRVEQERLRSLAPGEAAGQDPLSALSDLAVGLALGLMLEG
                     TALVPANGVDSRPTAYESLEWREMQAQLAAEVQKLPEKEAVIIRQHYHNGLSLAQIAD
                     LLDLSRGRISQLHRSALERLRKRIGAF"
     gene            11426..12025
                     /locus_tag="VF547_09430"
     CDS             11426..12025
                     /locus_tag="VF547_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263081.1"
                     /translation="MRFDRGEDMMDGLCSDAELQELMEVMQADSGDELEQADRLISDY
                     PEDPRLHFLRGSILASIGRPIEALPALRTAVELAPDFAIARFQLGFFQLTSGEAANAL
                     STWGPLALLPEQNYLRMFVAGLTHLIRDEFEEAITQLRAGMAANDENPPLNRDMQLIV
                     EQTSALAAGAATADTPDREAASATSFLLDQLSGGSGKAH"
     gene            12043..12384
                     /locus_tag="VF547_09435"
     CDS             12043..12384
                     /locus_tag="VF547_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263082.1"
                     /translation="MTRISQADHVLILLREQLQKIGRGRKERTSRSGTVRGATPRPIA
                     RLQAMGSLEEGEEFRRTLVRAILTEEMGEAMANDPSFQAVVDNVYRMISATPDGQELI
                     DRAGRQLRRSA"
     gene            complement(12397..13545)
                     /locus_tag="VF547_09440"
     CDS             complement(12397..13545)
                     /locus_tag="VF547_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263083.1"
                     /translation="MTASIDTAQADQPAPSRLERLMPLLDSDPSNLALLADVAETAMA
                     EQRPEVAAELLDRYAAIAPLPPRECNLAGLAALQTRRYDRAVELFGQLVDSGEDDPAV
                     RFNLAWACAMNKDFDRALELADDDLARALPQAAMLQVQILHDRGEFDDAADRAREYIE
                     IHPSYGPLMAAVSVLALDVEDPDLAAACAAKGGEHPDALATLGTLALGEERATEALEL
                     FDRALERNQQVPRAWVGRGLARLLTGASPEAAADIDRGAEMFGDHLGSWIAAGWAHFV
                     NKDMAAARARFETALELDANFAETHGSLAVLDLLEGDAEGARAKSEVALRLDRQCYSA
                     ALARTLLAASAGDQATAKRIYDAAVNAPIDGSGRTIGQALAKLGMGHG"
     gene            complement(13604..15637)
                     /locus_tag="VF547_09445"
     CDS             complement(13604..15637)
                     /locus_tag="VF547_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX8263084.1"
                     /translation="MLIQSLRNNKDLPLVIGMVLILMILFAPIPPAALDLAIITNFGF
                     GLTILLLTFYVGKPVEFSTFPSLLLVATLFRLSLSVAATRLILTGADAGDVINSIGTF
                     AVQGNFVIGLVVFCILIVVQYIVVTSGAQRVSEVAARFMLDSMPGQQMSIDADLNMGL
                     IDQKEAIRRREELEKEASFYGAMDGASKFVKGDAIAGIIILLINIIAGWIIGMVQMGM
                     AWDEALERFTLLTIGDGIATQLPALIIAIATGIIVTRSSADKELSTEVFRQLASVPRI
                     PLIVAIVLMALLLLPGMPKWPILVIIAGSIFAWYRARKSAAALAEAGESEAAESGEMA
                     SSGPAPALEIRLGSELAAAWKSEEALISDRIASLRRVHEQDFGIGFPGVRTVEGAGLG
                     TLEYEIRLFGARFGAAEIHPDRMLAIAPGSANGVKLEGIETRDPAFNLPALWIEPGAA
                     AEARQAGLKVIDPVTVLMTHFAEIVQSEMATLVTRGVVVKMLEDTRERQPGLLEELIP
                     NCLTISDVQRVLQNLLSEGVSIANLDLILEHLADLARSQKDPAELTEMIRQRLSYSIC
                     HQLRGKHHDLAVLSLDPRIENQISSNLSQSGASGMLPVEPRLADQLIRKLSTLAEDMH
                     REGRSPVLLCGAEIRRHLKAFTKRSVPRLSVLSVNEIPMRINLRSFDIIKLEA"
     gene            complement(15612..16715)
                     /locus_tag="VF547_09450"
     CDS             complement(15612..16715)
                     /locus_tag="VF547_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX8263085.1"
                     /translation="MAEGQEQNRSEEPTPFKLRRAREKGQVARGMDLGFVAGLVALAA
                     FAIVAGERLVADLAQLMRQALASGVTGRDPQEAAALAAALYWPAMRPLVLLGGTVVMI
                     VLLLEIVQLRGLVFSSHPLKPDFSRLNPAKGFKRIFSMRMVKEALKNVIKLAAYSIVT
                     TLLIWSLVSTPGRSQEGAEGLAGAMGGGAMRLLMLFILIALFFAAIDQIIVRREFHKQ
                     MRMSRREVTRETKDREGEPRIKQKRKQLHAEFSKQGRSMGALPGSDLLVVNPQHFAVA
                     LAYDPARMTAPTVAAKARNNYALAMKREAFRLGIPIFEDRPLARSLYARCETGTEVGA
                     SDYHAVAALYLKLHHARSTNQSKNENADSIPAQ"
BASE COUNT         2594 a         5937 c         5836 g         2530 t
ORIGIN      
        1 ggccaatgtc ggcgaccgta tcgacgccgt cgtctcctcg atcggcgatg cgcgcagcct
       61 cgccggcggc acgctgctga tgactccgct gatgggcccg gaccagcggc cctacgcgct
      121 cgcgcagggc tcgatcatca ccggcggcca cagcttcgag agcgagctca accagaacca
      181 gcgcaactat ccgaccagcg ccgttctcca gggcggcgcg accgtcgagc ggccggtcga
      241 tgcccagatc atgcggccgg gcggtgagct cgccttcctg ctcaacgacc cgaatttcgg
      301 gacggcccag cggatcgcca acggcatcaa cagccgcttc ggcgcgggct ccgcgacggt
      361 tcgcaacgcc gacgaggttc gaatccgctt cgccggcgat cccacgcaat tcgcggccta
      421 tgtggccgag ctcgaatcgc tgtccgttca gcccgagcgg ccgccgcgcg tcgtcatcaa
      481 cgagcgcacc ggcaccgtcg tcgccggggg cgacgtgatg atctccagcg tcgtcatctc
      541 gcagggggac gtgaaggtca ccgtgaccgc ggacaattat gcgtcgcagc ccgatttcta
      601 ctcgggcttc gccagcgacg tccgcagcct cgtcgtcacc aacaccaagc tcgacgtcga
      661 gcagggcgcc ggcgacgccg tggtcagctt cccgaacacg tccgtcgccg acctggtcca
      721 gggcctctac cgagcccggg tcaacacgcg ccgaacgatc agcatcctgc aggccatgaa
      781 ggccgccggc gctctgcacg ccgaactcat cgtccagtaa ggggattaag aacgtggatc
      841 cgacatcggc gatcatgatc accaaggcgc tcgacgggct gtgggcccgc tcgctcgcca
      901 cggcccagaa cgtcgccaat gcgaactcgc cggggtaccg gccggtgcgc gtcagcttcg
      961 aggaggcgct tcaggcggcg tcccgccagg gcccgggcgc gctcagcggc gtcagggcga
     1021 cggtcgggca ggcgccggcg ggcaccaagg gcgcggagct gaggctggat ctggagcttg
     1081 cgacggcgtc ggagaccggg ctgcgctacg ccgccctgct cgatctcctc ggccgccaat
     1141 tgcagatcag ccatacggtc atcaggggag gtcagtaaca tgcgtgcgat ggagatcagc
     1201 cggtctgggc tggacgtcga gtggcagcgg cttcaggtca tcgcccagaa tctggcgaac
     1261 ctgaacacga cgcgcaccgc gacgggcgag ccgttccggg cgatgcgcct gctttccggc
     1321 ccgcgcgccg acttcgcgca gatgctcagc ggcaagggca gcgcccagca gctgcgcggc
     1381 gtgcaggtga tggggatgga ggcgaacggc gccggcttcc ggcgcgtgca tgagcccaag
     1441 cacccccatg ccgacgcgga cggcttcgtc agctatcccg acatcgatca tgccggcgag
     1501 atgactctga tgatcaagac ctcgcggacc tacgaagcga acctcacggc catgaacatc
     1561 gcgcagcaga tgtacagccg cgccctcgag ctggggcgcg gccaatgacc ggcatcgaag
     1621 cgatcggggc gatcggcctc ggcaccggcg acgccgccat cagccgactc tcgctgacgg
     1681 cgccggtggc gcccgtccag accaatcagg tcggcttctc cgacgtcctc gccagcggca
     1741 tgcgccacgt cgagggcaag ctcgccaccg ccaacgagct cgtccagcgc ttcgcgctcg
     1801 acgattcggt gcctctccac caggtcacct acgcgctcga ggaagcgcgg atgtcggtcg
     1861 agctggcgat gcaggtccgc acgcggctcg tcgagagcta ccgcgaactc atgaacatgc
     1921 agatctaaga gctcgcggac catggaaacc atcactctcg tcccggctgg accgagccgc
     1981 cagatcaaga tgctgtcgct cgtcttcgcg gcgctcttcg tcctgcttgc gctcggctat
     2041 tatttcttca tccgcacgga atatgcggtc ctgtacagcg ggctgcgtcc ggccgacgcc
     2101 tcggcgatcg tcgccgagct cgacgccaag ggcgtcgcct accagctgaa ggaccagggc
     2161 accacgatca tggtgcccaa gggggaatcg gactcggtgc gtctggcgat cgtccgctcg
     2221 gatgcgccgc tcaagggctc gtccggcttc gagctgttca acaagtccga catgggcctc
     2281 accgacttcg cccagaagat caactaccag cgcgcgctcc agggcgagct ggcgcggacg
     2341 atcatgatga tggacgggat cgagaatgcc cgggtccatc tgaccatccc cgagcgcacc
     2401 ttgttccgcg gcaaccgcag cgagcccaag gcggcggtga ccctgaccct ccagcgcggc
     2461 aaggccgtgg acgaggcgcg ggtcgccggc atccagcggc tggtggcggc ggcggtcccg
     2521 gatctcaagc ttgccgacgt cgtcgtgctc gacgaggtcg gccggatcat cagctcgaac
     2581 gccgaagccc aggccgacct tccgcccgag ctggccgagc gcaacgccgt gcaggaatat
     2641 taccgggccc atatccgaag cgcggtcgag gcggtgctgc ccggcctggg cgccgacgcc
     2701 cgcgtgctga tcgtcccgac ggcgagcgcc gcggacaccg ggtgggagcc ctccggagcg
     2761 ccggccgagg ccgtgaagct gcaggcctcg tcccgcgatc cgtcggtgcg caacttccag
     2821 ctgcggatct cggtgatcac tccggcgccg ctcaacctcg aggaccagga tcgcgtcagg
     2881 gccgcgctga cgcgcgcggt cgggctgaag cctgagagcg gcgattcgct gcagttcggg
     2941 ctgaaggtcg cgccggcccg ggccggagtg ccgatcgctc cgccggtcgc cgccgccggc
     3001 accgtcgact accgcaaggc ggccggcatc ccgcagggta actcgacctg gctcatcgcc
     3061 ctggccgcgc tggtcgcgct cctcctcgcc gccatcgcgg tgcggtcccg gtcctccgga
     3121 atctcgaccg agcagcgcga tctcctggtg cttcggatcc gccgccagct cagcctgatc
     3181 gatgggactt ccgatgcgcg aggctgacac gctgaccggc ctcgccgagg agttgcgggc
     3241 gctcaccgcg cgggaacgcc gcggcgtgct ggcggcgctg tccccgttcg agcgcgccca
     3301 ggtcggtgcg ctgatcgagg cagcggccac gccggagccg gccgagccgc cggcgccgga
     3361 ctatagccgt ttctcgccct ggctcgcgcg gcatctgggt gccgacgaga ccggcggcgc
     3421 cgaccgcaac ctgaccgcgg cgacgcggcg gctgctggcc gaagcccgcg ccgagctcat
     3481 gagcggcccg agggcgtcga gccgggcgcc ggagccggcg ccgaagcgcc gttccctggc
     3541 cgacgcggtg gcgcaattgc tctcgccccg tcaggcgcac cgatgagcgg cctgatcaag
     3601 ggcggcgcgg cgcattcggt gcgtccgttc ctcgaccccg ccgagcttcc gcccgctccc
     3661 ggcggcgatc cgcgcctgga ggcgctcgag cgcgagaatg acgagctgcg cgcggcgatc
     3721 gcggcgcagc gcaaggcggg cgaggaggcg gtcgcggcgg cccgggccga gggcgagcgc
     3781 cagggcaggg ccgctgccga cgatgccgcc gacaagcgcc tgcagctggt gcgcaagggc
     3841 gtggacgcgg ccattgccgt ttgggagaag cggctggccg atctcgacga cctcgctccc
     3901 tccctcgccc gcgccgccct cgccaagctg ttcgacgaca acgaggacca ttcgcgcttc
     3961 gtcgccggcc tgatcgggcg ccagctgcgg ctgcttcgcc gggacagcgt ggtcgcgatc
     4021 cgggtcagcg tgcgcgattt cccggacgag caggcgctgt cgcagctcgg cgccgaggcc
     4081 gccaccggct cgatccgtat cctcgccgac cccgacctcg cgtcgggcgg ctgccgagtc
     4141 gatcttcagc tcggtcacat cgacatcggc accaagaccc aatggcccca gctggccgcg
     4201 ttcctcgaca agctggccga gccggaggcc ggcgagtgac cgcgcaccgc ttcctggcca
     4261 agctcgaccg agccgatttc gcgcgtccct acggctatgt ccgacgggtc gcgcccgggc
     4321 tggtggaagc gtccggcccc gccggccgac tcggcgaatt gtgcgagatc gaatgcgagg
     4381 acgagcccgc tttgctggcc gagatcgccg ccgtcgacga gcgccgcgtg gtcctgatcc
     4441 cgctcgagca gcaggcgacg atccggcccg acgcacgggt gacggcgcgg ccgatgcgcg
     4501 accgggtcgc ggtcggggac ggattcgccg ggagggcggt cgacgctctc ggccggccga
     4561 tcgacgggct gggcccgatc gccgccgacg cgcatgcgca gctcgccggc tccgtgccca
     4621 agccgatgca ccgggccgat ccgtcgcgcg tgcttccgac cggcattcgg gcgatcgacg
     4681 ggcttttgac gctagggcag ggccagcgca tcggcatctt tgccgccagc ggcgtcggca
     4741 agaccagcct gatggagcag ctcgcgacgc aggtggattg cgatcgctgc gtcctgtgcc
     4801 tggtcggcga gcgcggacgc gaggtcgagg cgatctggcg cctgctttcg ggccgcgagg
     4861 attctgagcg cttcacgatc gtcgccgcca cgtcggacga gagcgccgcg ctgcgcggcc
     4921 gctcggtgag ctacgcgctg gcgctggccg agcattggcg ggccgcgggc gaccatgtcc
     4981 tgctgatggt cgactcggtg acccggctgg ccatggctct gcgcgaggtc ggactcgctg
     5041 ccggagcccc gccgacggtg cgcgcttaca ctccgaacgt gttcagcgcc ctgccgcgcc
     5101 tcgtcgagcg gtgcggcgcg gtccggtcgg gcggcgccat caccgcgatc atgaccgtcc
     5161 tcagcgaaac cgacgatgtc gacgacccga tcgtcgagtt gatgaagtcg cttctcgacg
     5221 gccatatcgt gctttcgcgc tcgctcgccg agcagggcca tttcccggca atcgacgttc
     5281 cgcgcagcgt cagccgcggc gcccagcagc tggtcgtcag cgagcaggcg agcgcgatgc
     5341 gcgaggccgt ggcccagctc gccgcctacg aagaggcgcg ggtgatgatc gagagcggcg
     5401 tctacaagtc ggggaccaac ggcgccgtcg accgcgccat cgccaagcgc ggcgagatga
     5461 acgccttcct tcgacagggc cggacggaga atagcgcctt tccggaaacc ttgcggaagc
     5521 tgcgcaccgt caccgctgga gcgtccagcc atgcgtgacc gggtcaagct tccgctcatg
     5581 gtccggctgg cctccgtgca gcgggtgaag aaggcggccg cggaggcggc gctgatgtcg
     5641 gcgcgctcgg ccgaggcggc cgcccggacc gccgaggagg aggctcaggc gcgcacccag
     5701 gccgcgcagg ccgaatgggg cgaccatatc tcaggaagcg ccttctcccc cgagttcagc
     5761 cgggccctgg ccgcccgggt gatcgaaggc gagaaggcgg cgtccagcgc atcgctgcgc
     5821 cgggagaggg ctgccgagat cacggttcgg cgaaaggacg attggcaggc gtcggaggcg
     5881 cgcagccgct cgggagacga atctctccgg cagcttcggc gccgcgtccg gcgccggctc
     5941 gaggaggacc gcctggctga aatgagcgat cgagtgacct tcgactggag ccggcgatga
     6001 gcgcgggccc cgtcaccagc cgcccgccgg tggatcccgg gctcgcgcag cgccagcccg
     6061 gcgaggccgc gagcgagagc ggggagggcg gtgcgtcctt cgccgatctg ctcgcggcga
     6121 tgctggcgcc cggcgccgcc ccgctcgccg agccggggac ggccgcgccc gggactcccg
     6181 aagcggtgtt cgaccggctc gattcggccg agatcttcaa cgaggcgggc ctgttccgcg
     6241 gggccgcgcc gctggcggcc gcctcgctcg atccgggctt gggccggctt ccggggacgc
     6301 cgtcgccgtc gctgccttcg gacgcggccc tggaggtcga gccgtcggcg ctcgccggcc
     6361 ccggtcccgt cccggccgat gcggagccgg ggcctgcgtc ttcctccgcc cggttcgtgc
     6421 ttgacgccgc gctcaccggc tcgcctggcg ccggccccgc cgcttcggcc gccggagccg
     6481 ccggctccgc gccggcgccg cgcggccacc cggctccggc gcctatggcc tcgtcccagg
     6541 ctccggaaac cgccccggcg ggctccgccg acccggtccg cgcgtcgtcg cggagggcgg
     6601 tgatgctcgt gcaggcccat ctcgccaaag ccgcttcgac gtcggcgcag gtgagcgccc
     6661 aggccgtcga agccgggatc agcctggtgg tccgggccga caagctcggc cgcgaggagc
     6721 gcgaccgctt gcgggtcgag atcgccgagc tgctcttgag ccacggattc gcgatggccg
     6781 agatgactct caatggcgag gcctggcccg tgccgcacag gaaggacgac tgatgcaaac
     6841 cggatccgtt tcagccacgc agccgggcgg cgccgccgtg cagccgccgc gctcggcgca
     6901 ggacacggcg accaacgcct ttggcctcag cttcgagtcg ctgctgaaga tcatcctgac
     6961 ccagctgacc taccaggatc cgctgaagcc gatggacaat ttccagttcg tctcgcagct
     7021 cgcgcaattc tcgcagatcc agctcggcca gaccaccaac gaccggctcg ggtcgatggt
     7081 caccgcgcag gccaatctgc aggcggccgg cctgctcggc cgaaccgtcg acatcccggc
     7141 cggagccgcc accatcagcg ggaaggtgat gtcgatcgcg ttccagaacg gcgatccgcg
     7201 cctcaccatc gaaaccgccg acaagcagac catcagcaac atctcaatcg ccagcatttc
     7261 gcagatccgc tagggagaaa caggacatgt tcggttccat ctatgtcggt ctcagcggcc
     7321 tcaacgctta ttcgcggggc ctccagcaga tcagcaacaa cgtcaccaac ctgaacgcct
     7381 cgggcttcaa gggctcgacc gtcgccttca ccgacctggt ggggatcaac ggcaacggcg
     7441 gcgcgtccta ttcgggcggc gggggcggcg gcggcaacgg cgtcgagatc ggcgacaccc
     7501 gcatcgactt caagcagggc gagcttcgcc agaccgaccg cgacctcgat ctcgccatcg
     7561 acggcagcgg cttcctgctg ctgatgcgcg gcgaggaggt gctctacacc cggaccggca
     7621 gcttcgaggt cgacaaggac ggtttcatcg tcctcgccgg caccgagtat cggctcgcca
     7681 cgctcgacgg ctccggccgg ccggtgagcc tgtcgatcga cgcgtcgcgc accagctcgc
     7741 ccaagccgac gacgacgatc aagttcgccg acaacctctc ctcgacggcg acgacctacg
     7801 gcatcgccga cgtgaaggtc tacgacgcca acggcggtga gcacgtctgg cagatcaagt
     7861 tcgacaaggc cgagggcgcg ctcaacgagt ggaagatcac catcaccgac gacaaggcca
     7921 agaccgtcgg caccaagacc ctcaagttcg tccagggcca ggtcgatccc accaccaaca
     7981 agctcctctt cgaggacacc gaaggtggac tgtcggtcac cctcgacttc tcgcagaacg
     8041 tgacttcctt ctcggcgggc acggtctcca ctttgaggaa ggcctcgtcc gacggctaca
     8101 aggtcggagc gatcacctcg atcagcgtca acgacaaggg caagctcgag atcggctatt
     8161 ccaacgagca gaagaaggag ctcgggtcga tcgcgatcgc cgatttccgc gacccgcagg
     8221 cgctcgagca gcgcagcggc ggcctgttcc gcgacaccgg cctcgcccag cgcgagctga
     8281 cctcgagcga cgacaagcgc gtcggccggg tgctctcccg ccggctcgaa gcctccaacg
     8341 tcgatcttgc caaggcgttc ggggagctga tcctcatcca gcgcgggttc caggcgagct
     8401 cgcagatcat cagcgtctcg aacgatatga tccagcagct cttcggcatc cggggccagg
     8461 gctaatcgag tccgcatagg ggagtcatca aagtgaacat gcccttcagg acgtggctgc
     8521 cttcgggagc cctgggcggc ggcgcggtcg accggctgct ggcggagacg accggccaat
     8581 ggtcggacca atggttcgcg cggcggctga tgcgcgccgt cggggaaacc gcgtcgctcg
     8641 accgcgccga gctcgggcgt atgcgcgacc ttcagctgcg ctacgtcgag ggcggcctcg
     8701 cgctcgcgct cgaggacgac gcggcgaccg tgatcgcccg gatgatgctc gacgagtccc
     8761 cggaccgccg tgccgtgacg gaggccgacg aggagctggt cgaacggctc gcgaccggct
     8821 gcatcgacga tctgtgcaac cgcctggcgg gcgcgttcgg cctcggccga acgcagtcct
     8881 ggcgacgcgg cggaatcgac ggtctcccgt tcgcggaagc ctgcgtgttc gcgctcgggc
     8941 cgtccgacaa cgcgccgacc atccgagtgc tggtctcgca cgagctggtc gccgggatcg
     9001 tccgttcgtc cgccaagccc gcccgcaagg cgctgccgct gcggccgctg gccgaggccc
     9061 tcgcgaagca gagcgtcgac gtctccgccg tcgtcggacg gtgcgagctc agcctcggcg
     9121 agctcgccgg gcttgcgccg ggcgacgtgc tcgtcctcga cgcctccacc ggcggcacgc
     9181 tgccgctggc catcaacggg gacgcccgct cgggccgctg cgccatcgag caggaagacg
     9241 ggcagctccg cctcaagatc gtgaaacctc tgaccggaag tgcccgtgac tgaacccgct
     9301 tcgaagactc ccccgaccgc gaccgccccc aaggccgaca gcgcgctggg caacatcaat
     9361 cccaagctga tcgagacggt cagcgtgacg ctcgaggctt atcttggcga ggctcggatg
     9421 acggtcgccg acctcaccgc gctcaaggac aattcggtgg tgccgctcga cgcgcccctc
     9481 aaccaggcgg tcgagctgcg gctgaacggg gtcgcggtcg cccgcggcga gctggtcgcc
     9541 gtcggagaca gcttcgcggt caggctcacc gagatcgcca aatgatcagg cctgggccga
     9601 tggcgacgct ctgcgccggc gcggcctttc tcgctgcgtc gccggccctg gccggaggcg
     9661 ggcgcctcgg cggcggtcag ccgctcgagg tgtcgctggg acggatcgtg tcggcgctgg
     9721 tcatctgcat catcatcgcc gtcctcgccg tgctgttgat ccgccagcgg tccgggaaga
     9781 tcgacctgca cgccttgttc gggcggttcc aggtccggcc gcgcgcgatc gaggtcgtcg
     9841 agactcgccg gctgagcccc cacgccgaca tctgcgtcgt ccgcaacgcg ggacgcgaat
     9901 atctgctgct gctgctcgcc ggcaacgccc gcgtgctgcg cgaggaaccg gtcgtggcgc
     9961 ttcccgacgc cggcggagac gccctgtgag cctgaggcgc ctcgccgtcc gtctcggcgc
    10021 cgctttggcg gtgcttgccg cggccgagcc ggcgctcgcc caggcgacgg ccggagtcgg
    10081 cccggacgtc gcccgtaccg cgctcatcct caccttgctg gcggtcgtcc cggcgatcct
    10141 cgtcagcatg accagcttca tccgcatcgt catcgtcctg gcgatggtgc gccacgcctt
    10201 cgggatgccg gaaacccccc cgaacgcggt gctggtgagc ctttccctgt tcctcaccgc
    10261 cttcaccatg tcgccggtgc tgagcgagct gaaccgcgat accgtgcagc cgctgctggc
    10321 cggcaagatc ggcgtcgagc gcgcgatgga gaccggcagc gcgccgctgc gcaagttcat
    10381 gctgacccag acccgcgacg ccgacatcga gtcgatctac gccatctcga acacgccgct
    10441 tcccgagcgg cccgagcagg tcgagatcct caagctcgcc ccggccttca tcctcaacga
    10501 gctccgggtc gccttcacca tcggcttcgt cgtcctgctg ccgttcctgc tgatcgacct
    10561 cgtggtctcc agcatcctgc tgtcgctcgg catgatgatg gtcccgccca ctaccatatc
    10621 cctaccgatc aagctgctca tgttcgtcct gatcgacggc tggaagctgg tgctggaggg
    10681 agtgctcgga agcttcgggt gagccgcgcc gccctcccca ccggccttgc cctggtcgtc
    10741 cacccgaccc gcgccgaagc gtcgctgtgg cggcggctgc ggttcgaggc ggagaccgag
    10801 tgccgagaga cgctgttcaa ccgctacgtt ccgctcgccc gcgccatcgc ggccgccaag
    10861 ttccaccagc ggcggggccg cgccgccgag cgcagcgact gcgagcattt cgcctacgaa
    10921 gggctgctgc aggcgatcga ccggttcgat ccgctgctgg gcgtgccctt ttcggcctat
    10981 gcccggcgcc ggatcgtcgg cagcatcacc gacggcctcg ccaagatgag cgaggtcaac
    11041 gcccagatca gccatcgcca ccgcgtcgag caggagcggc tgcgttccct cgccccgggg
    11101 gaggccgcgg gccaggatcc gctgtcggcg ctgtcggacc tggcggtcgg cctggcgctg
    11161 gggctgatgc tcgaggggac ggcgctggtg ccggcgaacg gcgtcgacag ccggccgacc
    11221 gcctacgaat cgctcgaatg gcgggagatg caggcgcagc tcgccgccga ggtgcaaaag
    11281 ctcccggaaa aagaggcggt tatcattcgc cagcattatc ataatgggct aagcttggct
    11341 cagatcgccg accttctcga cctcagccgc ggccggatat cgcagcttca ccgctcggct
    11401 ctggagcggt tgcgcaagag gatcggtgcg ttttgaccgt ggggaggaca tgatggacgg
    11461 cctgtgcagc gacgccgagt tgcaggagct gatggaggtg atgcaggccg attcgggcga
    11521 cgagctcgag caggccgacc gcctgatctc ggactatccc gaggatcccc ggctgcactt
    11581 cctgcgcggc tcgatcctgg ccagcatcgg ccggccgatc gaggcgctcc cggcgcttcg
    11641 cacggcggtc gagctggcgc ccgacttcgc catcgcccgc ttccagctcg gcttcttcca
    11701 gctgacctcg ggcgaggccg ccaacgcgct ctccacctgg ggcccgctcg ccctgcttcc
    11761 cgagcaaaac tacctgcgga tgttcgtcgc cggcctcacc catctcatcc gcgacgaatt
    11821 cgaggaggcg atcacccagc tgcgcgccgg catggcggcg aatgacgaga atccgccgct
    11881 caaccgcgac atgcagctga tcgtcgagca gaccagcgcc ctggcggcgg gcgccgcgac
    11941 ggccgacacg ccggaccgcg aggccgcctc ggcgaccagc ttcctgctcg atcagctgtc
    12001 cggcggcagc ggcaaagcgc actgatttcg aggccggagc ggatgacccg gatcagccag
    12061 gccgatcacg tcctcatcct gctgcgcgag cagcttcaga agatcggtcg gggccgcaag
    12121 gagcggacga gccggagcgg caccgtccgc ggcgcgactc cgcgtccgat cgcccggctc
    12181 caggcgatgg gctcgctcga ggagggggag gagtttcgac ggaccctggt gcgcgcgatc
    12241 ctcaccgagg agatgggcga ggcgatggcc aacgacccgt ccttccaggc ggtggtcgac
    12301 aacgtctacc ggatgatcag cgcgaccccc gacgggcagg agctgatcga ccgcgccggc
    12361 cggcagctgc ggcgaagcgc ctgatcccag ccggcctcag ccgtggccca tgcccagctt
    12421 ggccaaagcc tgaccgatcg tccgcccgct gccgtcgatc ggcgcgttga cggcggcatc
    12481 gtagatccgc ttggcggtcg cctggtcgcc ggcgctggcg gcgaggagcg tccgcgccag
    12541 cgcggccgaa tagcattgcc ggtcgaggcg gagcgcgacc tcgctcttcg ctcgggcgcc
    12601 ctcggcatcg ccttcgagca ggtcgagcac ggcgagggag ccgtgggtct cggcgaaatt
    12661 ggcgtcgagc tcgagcgcgg tctcgaagcg cgcgcgcgcg gccgccatgt ccttgttgac
    12721 gaaatgcgcc cacccggccg cgatccacga gccgagatgg tctccgaaca tctcggcgcc
    12781 ccggtcgatg tccgccgcgg cctccggcga cgcgccggtg agcagccgcg ccagcccgcg
    12841 cccgacccag gcccgcggca cctgctggtt gcgctcgagc gcgcggtcga acagctccag
    12901 cgcctcggtg gcgcgttcct cgccgagggc gagcgtgccg agcgtggcga gcgcgtccgg
    12961 atgctcgccg cccttggccg cgcaggccgc ggcgaggtcg ggatcctcga cgtcgagcgc
    13021 cagcaccgag acggcggcca tcagcggccc ataggaagga tggatctcga tatattcgcg
    13081 ggcgcggtcg gcggcgtcgt cgaactcgcc gcggtcgtga aggatctgga cctggagcat
    13141 cgccgcctgg ggcagagccc gggcgaggtc gtcgtcggcc agctccagcg ctcggtcgaa
    13201 atccttgttc atcgcgcagg cccaggcgag gttgaagcgc accgccgggt cgtcctcgcc
    13261 cgagtccacg agctggccga atagttcgac cgcacggtcg tagcgccggg tctgaagcgc
    13321 ggcgaggccg gccaggttgc actcccgagg cggcagcggc gcgatcgcgg catagcggtc
    13381 gagcagttcc gccgcgacct ccggccgttg ctcggccatc gccgtctcgg cgacgtcggc
    13441 gagcagggcg aggttcgacg ggtcggagtc cagcaacggc atcagccgct cgagacggga
    13501 cggcgcgggc tggtcggcct gggcggtatc gatggaagcg gtcattggaa cgactctccg
    13561 tggagcggca gaagaccgcc ggatcggggc gaaggcaagc ggatcatgcc tcgagcttga
    13621 tgatgtcgaa cgagcgcagg ttgatgcgca tcgggatctc gttgaccgac agcaccgaca
    13681 ggcgcggcac gctccgcttg gtgaaggcct tgaggtgccg cctgatctcg gcgccgcaca
    13741 gcaggaccgg cgagcggcct tcgcggtgca tgtcctcggc cagggtggag agctttcgga
    13801 tcagctggtc ggcgagccgg ggctcgaccg gcagcatgcc cgaggcgccg ctctggctga
    13861 ggttgctgct gatctggttc tcgatccgcg ggtcgaggct gagcaccgcg aggtcgtgat
    13921 gcttgccgcg cagctgatgg cagatcgaat agctcagccg ctgccggatc atctcggtca
    13981 gctcggccgg gtccttctgc gaccgcgcca gatcggcgag atgctcgagg atgaggtcca
    14041 ggttggcgat cgacacgcct tcgctcagca ggttctggag cacccgctgg acgtcggaga
    14101 tggtgaggca gttggggatc agctcctcga gcaggccggg ctggcgctcg cgcgtgtcct
    14161 ccagcatctt caccaccacc ccgcgggtga cgagggtggc catctcgctc tgaacgatct
    14221 ccgcgaaatg ggtcatcagc accgtcaccg gatcgatcac cttgagcccg gcctggcggg
    14281 cttccgcggc ggcgccgggc tcgatccaga gcgccggcag gttgaacgcc gggtcgcggg
    14341 tctcgatgcc ctcgagcttg actccattcg ccgagcccgg cgcgatggcc agcatccggt
    14401 cgggatggat ctcggccgcg ccgaagcgcg ctccgaacag ccggatctca tattcgagcg
    14461 tgcccaggcc cgcgccctcg acggtccgca cgccggggaa gccgattccg aaatcctgct
    14521 cgtggacccg gcgcaggctg gcgatgcggt cgctgatcag cgcctcctcg ctcttccacg
    14581 cggcggcgag ctcgctgccg agccggatct cgagcgccgg cgcggggccg gaactcgcca
    14641 tctcgccgct ctccgccgcc tcgctctcgc cggcctcggc cagggcggcc gcgctcttgc
    14701 gggcccggta ccaggcgaag atcgagccgg cgatgatcac caggatcggc catttcggca
    14761 ttccgggcag gaggagcagc gccatcagca cgatcgccac gatcagcggg atccggggga
    14821 ccgaggcgag ctgccggaag acctcggtgc tgagctcctt gtcggccgac gagcgggtga
    14881 cgatgattcc ggtggcgatg gcgatgatca gcgccggcag ctgggtggcg atgccgtcgc
    14941 cgatggtcag cagggtgaag cgctcgagcg cctcgtccca ggccattccc atctgcacca
    15001 tgccgatgat ccagccggcg atgatgttga tcagaaggat gatgattccg gcgatggcgt
    15061 cgcccttcac gaacttcgag gcgccgtcca tcgcgccgta gaagctcgcc tccttctcga
    15121 gctcctcgcg gcggcggatc gcctccttct ggtcgatgag gcccatgttg aggtcggcgt
    15181 cgatgctcat ctgctggccg ggcatcgaat cgagcatgaa gcgcgcggcg acctcggaga
    15241 cccgctgcgc gcccgaggtg acgacgatgt actggacgac gatcaggatg cagaagacca
    15301 ccagcccgat gacgaaattg ccctggaccg cgaacgtccc gatcgaattg atcacgtctc
    15361 cggcgtcggc gccggtcagg atcagccggg tcgcggcgac gctgagcgac agcctgaaca
    15421 gcgtcgccac cagcagcagc gacgggaagg tcgagaactc gaccggcttg ccgacgtaga
    15481 aggtgagcag caggatcgtc aggccgaagc cgaagttcgt gatgatcgcc agatcgagcg
    15541 cggcgggcgg gatcggcgcg aacaggatca tgaggatgag caccatcccg atgaccaggg
    15601 ggagatcctt gttattgcgc agggattgaa tcagcatttt cgttcttcga ctggttggtg
    15661 gaacgggcgt ggtgaagctt gaggtagagg gcggcgaccg cgtgatagtc gctcgccccg
    15721 acctcggtgc cggtttcgca gcgggcgtag aggcttcggg cgagcgggcg gtcctcgaag
    15781 atcgggattc cgagccggaa ggcttcgcgc ttcatcgcca gcgcataatt gttgcgcgcc
    15841 ttggcggcga ccgtcggagc ggtcatccgg gccggatcgt aggcgagcgc gacggcgaaa
    15901 tgctgcgggt tgacgaccag caggtcggat ccgggaagcg cccccatcga ccgtccctgc
    15961 ttcgagaatt cggcgtgcag ctgcttgcgc ttctgcttga tccgcggctc gccctcgcgg
    16021 tccttggttt cgcgcgtcac ttcgcgccgg ctcatccgca tctgcttgtg gaactcgcgc
    16081 cggacgatga tctggtcgat cgcggcgaag aacagggcga tcaggatgaa cagcatcagc
    16141 agccgcatcg cgccgccgcc catcgctccg gccagcccct cggcgccctc ctgcgaccgg
    16201 cccggagtgg acaccagcga ccagatcagc agggtggtga cgatcgaata ggcggcgagc
    16261 ttgatgacgt tcttgagcgc ctccttgacc atccgcatcg agaagatccg cttgaagccc
    16321 ttggccgggt tgagccggct gaaatcgggc ttcagcggat gcgagctgaa gacgagtccg
    16381 cgcagctgca cgatctcgag cagcaggacg atcatcacca ccgtgccgcc gagcagcacc
    16441 agcgggcgca tcgccggcca gtagagcgcg gcggccagcg cggccgcctc ctgcgggtcc
    16501 cggccggtca ctccgctggc cagggcctgg cgcatcagct gcgcgaggtc ggcgaccagc
    16561 cgctcgccgg cgacgatggc gaatgccgcc agagcgacaa gcccggcgac gaagccgaga
    16621 tccatgccgc gcgcgacctg ccccttctcc cgcgcccgcc tgagcttgaa cggggtcggc
    16681 tcctcgcttc gattctgttc ctgcccctcg gccatcagtc gcgacccatc agatcagctc
    16741 cgcggccgtc tgcagcgcgt agcgcagcat tcgaaggaag agcgcggccg acaaggcgat
    16801 tccgatcggg aggatggcga gcaccgcgat cgtcttcacc tggaagccga gcaggagcac
    16861 gttcatctgc ggcagggtgc gcgacatgaa ggcgatg
//
LOCUS       DASVQZ010000333        11378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_266979, whole genome shotgun sequence.
ACCESSION   DASVQZ010000333 DASVQZ010000000
VERSION     DASVQZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11378
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266979"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1506)
                     /locus_tag="VF547_09455"
     CDS             complement(133..1506)
                     /locus_tag="VF547_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX8263086.1"
                     /translation="MTRFILTADEMRAAEAAVIAAGTPGLTLMERAGAAAAESIWRFA
                     GPLPVLVLCGPGNNGGDGYVIARRLAGRGIAARVAALAEPRSADAKAARAAWTGPVET
                     LDEASPAPLLVDALFGTGLARPLDASSSLRLRALAGAARVKVAVDLPSGVATDDGGIL
                     SPVSDFDLTIAFAALKPSHLLQPAARHMGRTVVADIGIAARSRLRVIARPRLRRPGPD
                     DHKYKRGYLLVVGGAMPGAAALAAGAAARAGAGYVALVGGGGSVPDAVVRRPPAELDE
                     ALADPRLNAVLVGPGLGRQTEARETLERALSSRHPAIVDGDALRLMADSVVPLSRAAP
                     CIATPHAGEFEALFGDKGSKVEAARAAAGRTGMTIVYKGADTVVASPDGEAAIGAPAP
                     HWLAGAGTGDVLAGIIAAQCAAGLGPFEAACAGVWLHGRAAELAGPALIADDLPDRLP
                     AAFAECL"
     gene            complement(1503..1832)
                     /locus_tag="VF547_09460"
     CDS             complement(1503..1832)
                     /locus_tag="VF547_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263087.1"
                     /translation="MKRVAVLLLLLLTACGGPDGSEAVARIECAVDGSTVFARVCTVE
                     RSAGPEAVFTIRSPSGSFRRLVATRDGRGVMAADGAEAATVTVIGTGLIEVTIGTDRY
                     RLPATVR"
     gene            complement(1871..2608)
                     /locus_tag="VF547_09465"
     CDS             complement(1871..2608)
                     /locus_tag="VF547_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8263088.1"
                     /translation="MFDLTLDGPVARLRLDRPEARNAIPASAWAELARSCAEAAASGA
                     RLIVVAGTAKAFCAGADLADFPDLAVDEARAAGFRRAMRLGLDALRDVPLATIAAIEG
                     PCYGAGVALAIACDMRVAGRAARFAVTPAKLGISYPQEDVARLVALIGPGQASRLLLG
                     AGSIDSAEAARIGLVDLEAGNLDETVAELASAICANSGESVAVLKRAIGLAAAGVARD
                     EEQDRRFDAMFGTADFRERLAALRPRR"
     gene            complement(2870..3640)
                     /locus_tag="VF547_09470"
     CDS             complement(2870..3640)
                     /locus_tag="VF547_09470"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX8263089.1"
                     /translation="MEEIAYAKINLALRVLRRREDGYHDIDTLFAFLRDGDRLSVAPA
                     DELKLRVVGPFAGDLGEAEDNLVLRAARLLGGDRGAALTLDKRLPVASGIGGGSADAA
                     AALRLLARWWRLEEMDLMALARRLGADVPACLASRTVRGTGRGDRLIGVRQSFAGTPA
                     LLVNPGVAVATAEVFGRWKGVRARGNDLEPPALEIAPAIGEAIELLHAQPGLRMARMS
                     GSGATCFGLFATDAQRDSADAVIAEARPDWWRLASRLR"
     gene            complement(3640..5277)
                     /locus_tag="VF547_09475"
     CDS             complement(3640..5277)
                     /locus_tag="VF547_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263090.1"
                     /translation="MTICALLSTTPASATLEESSALAAYVRARAADSSGAAEQAARNY
                     GAALALAPQNEVLAARALAQAIAAGDRPLALQAARTLEAAGKLAPDGRLLLVGEALRS
                     KQWKEAGRQIDRLDQDEVFSFMTPLLRAWLAQGSGKGDPLKLIEATSGNALAATYASE
                     HRPLLLIARGKAKEGAAALAPLLQDESIRSQRVRIAAAATLARKGARKEALTLLEGQA
                     EALAEARRRLAAGKRPGGEIASPAAGMSELLARMALDLNGQEVPQLALSFARLATFLA
                     PESAEAWLIAADLLAGQGRHEAALAALVKVPIDDVFAATASDRRMTILVDANRTDEAL
                     AEARRAAEDGPAAIDNWTRLGDILNQSQRHSEAAAAYAKALDLAKSGVEAQHPLWALW
                     LLRGSALTQAGDWTQGKAALEEARRLAPDQAVVLNFLGYSQLERRENLAEAEKLIGEA
                     SRLQPDDAAITDSLGWAHYVRGDVPRAIELLERAAQGQPADAAIAEHLGDAYYSAGRR
                     FEARYAWQAALSYAQGAAAERLRSKIDTGLRPDLAAP"
     gene            complement(5366..7012)
                     /locus_tag="VF547_09480"
     CDS             complement(5366..7012)
                     /locus_tag="VF547_09480"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX8263091.1"
                     /translation="MSERESMPYDVVIVGAGPAGLAAGIRLKQLASEAGRELTVCILE
                     KGSEVGAHILSGAVIDPRAVDELLPEWRELGSPLTVPVVENHHWVLTRTGKYSLPHLL
                     MPPFMNNKGTYTASLGNFCRWLAGQAEALGVEIFPGFAAAEVLFNDDGSVKGVATGDM
                     GVARDGTRRADYQPGMELHGRYTFFAEGARGHLTKQLKRIFDLERDSQPQVYGIGLKE
                     LWDVEPGKHVPGRVIHTQGWPLDDAWGGGFLYHQENNQVALGFVVALNYRNPHLSPFE
                     EFQRWKQHPAIREVLEGGRRVSYGARAINEGGWQAVPKLAFPGGALIGCSAGFVNVPR
                     IKGSHTAMKSGMLAAEAAFAAISADRSGDELETYEESLRNSWIATELKTVRNVEPLVA
                     RFGGAMGTLLAGTDMWMRTLRIGLPFTLRHHPDNETLYRRDLVPPIDYPKPDGVISFD
                     RLSSVFISNTNHEEDQPVHLTLKDPAIPTEVNLPLYDGPEQRYCPAGVYEFVEEASGP
                     RLQINAQNCVHCKTCDIKDPTQNINWVVPEGGGGPNYPNM"
     gene            7151..7858
                     /locus_tag="VF547_09485"
     CDS             7151..7858
                     /locus_tag="VF547_09485"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8263092.1"
                     /translation="MSPAEAESALSWWAEAGVDTLVDEAPRDWLRPKPREAPAPVPAA
                     AEIPDSNLPGQLDLFQAWLASSDRLAFAAPAAPRICPAGDPASGLMILADMPSGEDCA
                     AGTLISGEAGRLFDRMLAAIGRDRQSIYLAALSCIRSPSGQLNGEAMKSCAALARHHV
                     GLAAPKAVLLFGDACAKALLGLSVPQARGRWHELATHAGPVKALVTIAPRQLLDSPRL
                     KALAWADLQMLIEEVKE"
     gene            7855..9594
                     /locus_tag="VF547_09490"
     CDS             7855..9594
                     /locus_tag="VF547_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8263093.1"
                     /translation="MKFSHLLLAAALALPAAPALADGGSTLSSGLSAEQRSAWREVFA
                     GLRAEDWTGVAARLAALPDGPLHRAARAELYLAKGSPKVELPQLLSLLEAAPEMPRAE
                     QLARLALTRGATELPPLPQAQSLAWVGSQPRRGRAQSTRDDPAAAELEPLVQPLIKDN
                     RPHEAEAIFLQHQDRLGPDARTEFQQRIGWSYYTIGADRDARRLAALARAGTGEWAIQ
                     GAWSEGLAAWREGDCRGAAEAFDTVAGRSGDVELVAAAHYWSARAEMMCGRPERVQGH
                     LRGAARQKETFYGLLANTALGVTAPGASGLHNYRDAEWRGIAGKPNVRAALVLTEIGE
                     TDLADQFLRHQARIGGAADHNALLHLAAELDLAATQYWLAHNAPRGTSVNFAARYPRP
                     DWRPVRGWRVDPSLAFAHALQESNFRTSVVSPAGAVGVMQVRPGTAGDIARARGEPFD
                     RQLLTLPAANIEFGQSYIESLRDHGATGGLLPKVIAAYNAGPVPVAEWNARRFDRGDP
                     LLYIESLPYWETRGYVPIVLRNYWIYEEAAGKRGDSREALAQGLWPKFPGMPGARAVR
                     LKPDETKLAYGTE"
     gene            9664..10749
                     /locus_tag="VF547_09495"
     CDS             9664..10749
                     /locus_tag="VF547_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX8263094.1"
                     /translation="MHRPDVRHGRGAPENRTPTRFNLPVREADGDWLDAREAVDDGPP
                     PLRTTVTVEKPRSIIAWNRSPDIGFDRSINSYRGCEHGCIYCFARPTHAFHDLSPGLD
                     FETKLFAKPDAAKILREELAKPGYVVRPMAIGTNTDPYQPIEGKWRITRSVLEVLAET
                     RHPVTITTKSDRVLRDLDLLARMAAEGLASVALSVTSLTPAISRTLEPRAPSGLKRLA
                     AVKTLAEAGVPATVAIAPVIPGITDHEVEHIVEAAAEAGAVGAFFLPVRLPHEVAPLF
                     RAWLDEHFPDRADKVMNIIRSMRGGKDNDPNWFTRMSGSGPWAELMRVRFEVAAKKLG
                     LNRDKRKLRCDLFAPPTGPQGELFGRI"
     gene            10828..11220
                     /locus_tag="VF547_09500"
     CDS             10828..11220
                     /locus_tag="VF547_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263095.1"
                     /translation="MRFILSGIPPESRVDQGPAQLGVSVGSRFGNLGVAYQLALGEVV
                     TIEGATDDQSSLTARLLIGNDEVEFSIDNVDEDSDVIISPLSEKVFFVNVWHRQRPAP
                     CIVRCADGTVGQPCVTCRKGNRVVRICC"
BASE COUNT         1649 a         4069 c         4027 g         1633 t
ORIGIN      
        1 gccggtgcgg tccgggcagg accgatccgt cggccagcac ccggtccccg ggggcggtca
       61 gcggaaagaa gcggccgcgg tcgccgaggc cgtcgccgcg cccggcaagg cggacgatcg
      121 tctccggctc gctcacagac attcggcgaa ggccgcgggc aggcggtccg gcagatcgtc
      181 ggcgatcagc gccggaccgg cgagctcggc ggcgcggccg tgcagccaca ctccggcgca
      241 ggcggcttcg aacggcccca gtccggccgc gcactgggcg gcgatgatgc cggcgaggac
      301 gtcgcccgtg ccggcgcccg cgagccagtg gggcgccggg gcgccgatcg ccgcctcccc
      361 gtccggcgaa gccacgaccg tatcggcgcc cttgtagacg atcgtcatcc cggtccgccc
      421 ggcggcggcg cgcgccgcct cgaccttgct ccccttgtcg ccgaacagag cttcgaactc
      481 gccggcatgc ggcgtcgcga tgcacggtgc ggcccggctc aacgggacaa ccgagtccgc
      541 catcagccgc agggcgtctc cgtcgacgat cgccggatgc cggctcgaca aggcccgctc
      601 gagagtctct cgggcctcgg tttgccgccc gaggccgggg ccgaccagga ccgcgttcaa
      661 gcgcgggtcc gccagcgcct cgtccagctc cgccggcggc cggcgcacga ccgcgtcggg
      721 aacgcttccg cctcctccga ccagcgccac atagcccgcc ccggcgcggg ccgcggcgcc
      781 ggccgccagc gccgccgcgc cgggcatcgc gccgcccacg accagcagat agccgcgctt
      841 gtacttgtgg tcgtcgggcc cgggccggcg cagcctcggt cgggcgatga cgcgcagcct
      901 gctccgggcg gcgatgccga tgtcggcgac gacggtccgc cccatgtgcc gggcggccgg
      961 ctgcaggagg tgcgagggct tcagggccgc gaaggcgatc gtcaggtcga aatcggagac
     1021 gggggagagg attccgccgt catcggtggc gaccccgctc ggcaggtcta ccgccacttt
     1081 cacccgtgcc gccccggcga gcgcgcggag ccgaagcgat gaggaagcgt cgagcgggcg
     1141 ggcgagcccg gtgccgaaca gggcgtcgac caggagcgga gccggcgacg cttcgtcgag
     1201 ggtctcgacc ggccccgtcc aggcggctcg cgccgccttc gcgtccgcgc tcctcggctc
     1261 ggcgagcgcg gcgacccggg cggcgatccc gcgtccggcg aggcggcgcg cgatcacata
     1321 gccgtcgccg ccattgttgc ccggaccgca caggaccagg accggaagcg gtccggcgaa
     1381 tcgccagatc gattcggcgg ccgcggcgcc ggcgcgctcc atcaacgtca ggccgggcgt
     1441 cccggcggcg atgacggccg cctcggcggc gcgcatctcg tccgcggtga ggatgaagcg
     1501 ggtcatcgca ccgtcgcggg caggcggtag cggtccgtgc cgatcgtcac ttcgatcagg
     1561 ccggttccga tcaccgtcac cgtcgccgcc tcggcgccgt cggcggccat cacgccgcgt
     1621 ccgtcgcggg tcgcgacgag ccgccggaag ctgccgctcg ggctgcggat ggtgaacacg
     1681 gcctccgggc cggcggagcg ctcgacggtg cagacgcggg cgaagacggt cgagccgtcg
     1741 accgcgcatt cgattcgcgc gacggcttcg gacccgtcgg ggcccccgca ggccgtaagc
     1801 agaagaagga gaagaaccgc cacgcgcttc atgcgcccgg cttgtcccct attgccgccg
     1861 ggcaggcaag tcagcggcgg ggccggagcg ccgccagccg ttctcggaaa tcggccgttc
     1921 cgaacatcgc gtcgaagcgg cggtcctgct cctcgtcgcg ggcgacgccg gcggcggcca
     1981 ggccgatcgc gcgcttcagc acggcgacgc tctcgccgct gttcgcgcag atcgcgctcg
     2041 ccagctccgc cacggtttcg tccaggttgc cggcctcgag gtcgaccagg ccgatcctcg
     2101 ccgcctcggc cgagtcgatg cttccggcgc cgagcagcag cctggaagcc tggccggggc
     2161 cgatcagggc gacgaggcgc gcgacgtcct cctggggata ggatatgccc agcttggccg
     2221 gcgtgaccgc gaaccgagca gcccttccgg ccacgcgcat gtcgcaggcg atggcgaggg
     2281 ccacgcccgc gccgtagcac gggccctcga tcgcggcgat ggtggcgagc ggcacgtctc
     2341 gaagcgcgtc cagccccaga cgcatcgccc gccggaagcc ggccgcccgg gcctcgtcca
     2401 cggccaggtc ggggaaatcg gccaagtccg ctccggcgca gaaagccttg gcggtgccgg
     2461 ccacgacgat caggcgggcc cccgacgcgg ccgcttcggc gcagcttcgc gcgagctccg
     2521 cccaggccga cgccggaatg gcgttgcgcg cctccgggcg gtcgagccgc aggcgggcga
     2581 cgggcccgtc cagggtcaga tcgaacatcg gccgatttga cggcgcgcgg cggctttgtc
     2641 gccacaaaaa agagccggcc gcccggtcgg gagcggccgg ctctcagggg agccatggcg
     2701 ccggctccgc gcgccgcgga gcgggaccat gtgcgaatgt ccacaagccc cctcaaggcc
     2761 ctgtttcgac tcgtcgccgc tcccgcagca aggagcgtgc cacgacgcaa gactgcgggc
     2821 tgcgatggtt aagggctgcg gaaggacgtc ccggagcggg actcgcgggt cacctcagac
     2881 ggctggcgag ccgccaccaa tccggacggg cctcggcgat gacggcatcc gccgagtcgc
     2941 gctgagcgtc ggtggcgaac aggccgaagc aggtcgctcc cgagccggac attcgggcca
     3001 ttcgcaggcc gggctgggca tgaagcagtt cgatcgcctc gccgatcgcg ggcgcgatct
     3061 ccagcgccgg cggctccagg tcattgccgc gggcccgaac gcccttccat cgcccgaaca
     3121 cctcggcggt ggcgacggcg acgcccggat tcaccagcag cgccggcgtg ccggcgaaac
     3181 tctgccgaac cccgatcagt cggtcgcctc ggcccgtgcc ccggacggtt cgcgatgcca
     3241 ggcacgccgg cacgtcggcc cctaggcgtc tggccagggc catgaggtcc atctcctcga
     3301 gcctccacca gcgcgccagc agccgaaggg cggccgcggc gtcggcggag ccgccgccga
     3361 tgcccgatgc gaccggcagc ctcttgtcga gcgtcagcgc ggcgccccga tcgccgccga
     3421 gcagacgggc ggcgcggagg accagattgt cctcagcctc gccgaggtcg cccgcgaacg
     3481 gaccgacgac ccgcagcttc agctcgtccg ccggcgccac gctcaggcgg tcgccgtccc
     3541 gcaggaaggc gaacaaggta tcgatgtcgt ggtagccgtc ttcccgccgc ctgagcaccc
     3601 gcaacgcgag gttgatcttg gcgtaggcga tctcctccat cagggtgcgg cgagatccgg
     3661 ccgaaggccc gtgtcgatct tgctgcgcag tcgctccgcc gcggcgccct gggcgtagga
     3721 aagcgccgcc tgccaggcgt atcgggcctc gaagcggcgg ccggcgctgt aataggcgtc
     3781 gcccagatgc tcggcgatcg ccgcgtcggc gggctggcct tgcgccgcgc gctcgagcag
     3841 ctcgatcgcc cgggggacgt cgccccggac gtaatgcgcc cagccgagcg aatcggtgat
     3901 cgcggcgtcg tcgggctgga gccggctcgc ctcgccgatc agcttttccg cctcggcgag
     3961 attctcgcgc cgctcgagct gcgaatagcc gaggaagttg agcacgacgg cctggtcggg
     4021 cgcgagccgc cgcgcctcct cgagcgcggc cttgccctgc gtccagtcgc cggcctgggt
     4081 cagagcgctg ccgcgcagca gccacagcgc ccacaggggg tgctgcgcct cgacgcctga
     4141 cttggcgaga tcgagcgcct tcgcataagc ggcggcggcc tcggaatggc gctgcgactg
     4201 gttgaggatg tcgccgagcc gagtccaatt gtcgatcgcg gcggggccgt cctccgccgc
     4261 ccggcgcgcc tcggcgaggg cctcgtcggt tcggttggcg tcgaccagga tggtcatccg
     4321 gcggtcggag gcggtcgcgg cgaagacgtc gtcgatcggc accttgacga gcgcggcgag
     4381 cgccgcctcg tgccggccct gccccgccag caggtcggcc gcgatcagcc aggcctcggc
     4441 gctctccggc gccaggaagg tggcgagccg cgcgaagctg agcgccagct gcggcacttc
     4501 ctggccgttg aggtcgagcg ccattcgcgc gagcagctcg gacatgcccg ccgcaggcga
     4561 cgcgatctcg ccgcccggcc tcttgccggc cgcgagccgc cggcgggcct ccgcaagggc
     4621 ttcagcctgg ccctcgagca gggtgagcgc ctccttgcgg gcgcccttgc gggcaagggt
     4681 cgccgccgcc gcgatgcgca cgcgctgcga gcggatcgat tcgtcctgga gcaggggcgc
     4741 aagcgccgcc gcgccttcct tggccttgcc ccgggcgatg agcagcagcg ggcggtgctc
     4801 cgaggcgtag gtcgccgcga gggcgttgcc ggacgtcgcc tcgatcagct tcagcggatc
     4861 gcccttgccg ctgccctgcg ccagccaggc gcgaagcagc ggcgtcatga acgagaagac
     4921 ctcgtcctgg tcgagccggt cgatctgacg gcccgcctcc ttccactgct tcgagcggag
     4981 cgcttcgccg accagcagca gccgcccgtc gggggcgagc ttgccggcgg cctcgagcgt
     5041 ccgggcggcc tgcagcgcca gcgggcgatc gcccgccgcg atcgcctggg cgagcgcgcg
     5101 cgcggcgaga acctcgttct gcggcgccag cgccagcgcc gcgccgtaat tgcgggccgc
     5161 ctgctcggcc gccccggagc tgtcggcggc tcgggcgcgg acgtaggcgg cgagcgcgga
     5221 ggactcctcg agcgtcgctg aggcgggggt cgtggacagg agcgcacaga tcgtcatccc
     5281 ggcgaaggcc gggatcccag cgagcgaaag gcgacggcgg tcctcaccgc ctggggctcc
     5341 agtcttcgcc ggcgcgacgg ggttcttaca tattgggata attcggtcct cctccgcctt
     5401 ccggcacgac ccaattgatg ttctgggtcg ggtccttgat gtcgcaggtc ttgcagtgaa
     5461 cgcaattctg ggcgttgatc tgcaagcgcg gcccactcgc ctcctcgacg aactcgtaga
     5521 ctccggccgg gcaatatctc tgctccggcc catcgtagag cggcaggttc acctcggtcg
     5581 ggatcgcggg atccttgagg gtgaggtgga ccggctggtc ctcctcatgg ttggtgttgg
     5641 agatgaacac cgaggaaagg cggtcgaagc tgatcacgcc gtcgggcttg ggatagtcga
     5701 tcggcgggac gagatcgcgg cggtagaggg tctcgttgtc cggatggtgc ctcagcgtga
     5761 acggcagccc gatcctcagc gtgcgcatcc acatgtcggt gccggcgagc agcgtgccca
     5821 tcgcgccgcc gaaccgcgcg accaacggct cgacattgcg caccgtctta agttcggttg
     5881 ctatccagct gttgcgaagc gattcctcat aggtctcaag ctcgtctccg gagcggtcgg
     5941 ccgagatcgc cgcgaacgcc gcttcggcgg cgagcatgcc cgatttcatc gccgtgtggc
     6001 tgcccttgat gcgcggcacg ttgacgaagc cggccgagca gccgatcagc gctcctcccg
     6061 ggaaggccag cttcggcacc gcctgccagc cgccctcgtt tatggcccgg gcgccgtagg
     6121 agacccggcg gccgccctcg agcacctcgc ggatcgccgg atgctgcttc cagcgctgga
     6181 actcctcgaa cggcgagagg tgcgggttgc ggtagttgag cgcgacgacg aagcccaggg
     6241 cgacctggtt gttctcctgg tggtagagga agccgccgcc ccaggcgtcg tcgagcggcc
     6301 agccctgggt gtggatgacc cggcccggga cgtgcttgcc gggctcgacg tcccacaatt
     6361 ccttgaggcc gatgccgtag acctggggct ggctgtcgcg ctcgaggtcg aagatccgct
     6421 tcagctgctt ggtgagatgg ccgcgcgccc cctccgcgaa gaaggtgtag cggccgtgaa
     6481 gctccattcc cggctggtag tcggcgcggc gggtaccgtc ccgggcaacg cccatgtcgc
     6541 cggtcgcgac gcccttcacc gagccgtcgt cgttgaacag cacctcggcg gcggcgaagc
     6601 cggggaagat ctccaccccg agcgcttccg cttggccggc cagccagcgg cagaaattgc
     6661 cgagggaggc ggtgtaggtg cccttgttgt tcatgaaggg cggcatcagc aggtggggaa
     6721 gtgaatattt gccggtgcgg gtaagcaccc aatgatgatt ttcgacgacg ggcacggtga
     6781 gcgggctgcc cagctcgcgc cactccggca gcagctcgtc gacggcgcgc ggatcgatga
     6841 ccgcgccgga gaggatgtgg gcgccgacct ccgagccttt ctcgaggatg cagacggtga
     6901 gctcgcgtcc cgcttcggag gcgagctgct tgaggcggat gcccgcggcg aggccggccg
     6961 gaccggcgcc gacgatcacc acgtcatagg gcatcgactc gcgttcggac atcaagcgct
     7021 cctcgccggg ccgcgcgcgg actcgcgtcg tcgcaggcag ccaacgtctg gcagatagat
     7081 agagattttg aggttgacca gcccgcccgc ccaggactcc atgcggtacg tggagcgggg
     7141 aaaccgaaca ttgtcgccgg cggaggccga gagcgcgctg agctggtggg ccgaggcggg
     7201 cgtagacacg ctcgtcgacg aggcgcctcg cgactggctt cggccgaaac ccagggaagc
     7261 gccggcgccg gttccggccg ccgccgagat ccccgattcg aacctgcccg gccaactcga
     7321 cctcttccag gcgtggctgg cttcgagcga ccgcctcgcc tttgccgctc ccgccgctcc
     7381 ccgcatctgt ccggccggcg atcccgcctc ggggctgatg atcctggccg acatgccgag
     7441 cggcgaggat tgcgccgcgg gcacgctgat cagcggcgag gccggccggc tgttcgaccg
     7501 gatgctcgcc gcgatcgggc gcgaccgcca gtcgatctat ctggccgctt tgtcctgcat
     7561 ccgctcgccg tcggggcagc tgaacggcga ggcgatgaag agctgcgcgg cgctcgcccg
     7621 gcaccatgtc ggcctcgccg cgccgaaggc cgtgcttctg ttcggcgacg cctgcgccaa
     7681 ggcgctgctc ggcctgtcgg tgccgcaggc gcgcggacgc tggcacgagc tcgccacgca
     7741 cgcgggcccc gtcaaggccc tggtaaccat tgcgccgcga caattgctgg actcgccccg
     7801 cctcaaggct ctggcctggg cggacctcca gatgctgatc gaagaggtga aggaatgaag
     7861 ttctcccacc tgctgctcgc ggccgccctg gccctgcccg ccgcgcccgc gcttgccgac
     7921 ggcggcagca ccctctcctc gggcctgagc gccgagcagc ggagcgcgtg gcgcgaggtc
     7981 ttcgccggtc tgcgcgccga ggattggacc ggcgtggcgg ctcggctcgc cgcccttccc
     8041 gacggtcccc tccaccgggc ggcccgggcc gagctttacc tggcgaaggg ctcgcccaag
     8101 gtcgagctgc cccagctgct gtccctgctg gaggccgcgc ccgaaatgcc ccgggcggag
     8161 cagcttgcgc gcctcgctct gacgcgcggc gcgaccgagc ttccgcccct gccccaggcg
     8221 cagagcctgg cctgggtcgg cagccagccg cgccgcggcc gggcgcagtc gacccgggac
     8281 gacccggccg cggccgagct cgagccgctg gtccagccgc tgatcaagga caaccggccg
     8341 cacgaggccg aagcgatctt cctgcagcat caggaccgcc tcggaccgga tgcccgaacc
     8401 gagttccagc agcggatcgg ctggtcctac tacacgatcg gcgccgatcg cgacgcccgc
     8461 cggctcgccg cgctggcccg cgccggcacc ggcgaatggg cgatccaggg cgcctggtcc
     8521 gagggcctgg ccgcctggcg cgagggcgat tgcaggggcg ccgccgaggc gttcgacacc
     8581 gtcgccggcc gctccggcga tgtcgagctg gtcgccgccg cccattattg gagcgctcgc
     8641 gccgagatga tgtgcggccg gccggaacgc gtccagggcc atttgcgcgg cgccgcgcgc
     8701 cagaaggaga ccttctacgg cctgctcgcc aacaccgccc tcggggtgac cgcccccggc
     8761 gcctccggac tgcacaatta tcgcgacgcg gagtggcgcg gcatcgccgg caagcccaac
     8821 gtccgcgcgg cgctggtcct gaccgagatc ggcgagacgg accttgccga ccagttcctg
     8881 cgccaccagg cccgaatcgg cggcgccgcc gaccacaatg cgctccttca cctcgccgcc
     8941 gagctcgacc tcgccgcaac gcaatattgg ctggcgcaca acgcgcctcg gggaacgagc
     9001 gtcaacttcg cagcccgcta tccgcgcccc gactggcggc cggtccgcgg ctggcgggtc
     9061 gatccttcgc tcgccttcgc ccacgccctg caggaatcga acttccgaac ctccgtggtc
     9121 agccccgccg gagccgtcgg ggtcatgcag gtccggcccg gcaccgccgg cgacatcgcc
     9181 cgggcccgcg gggaaccgtt cgatcgccag ctgctcaccc ttcccgccgc caatatcgaa
     9241 ttcggccaga gctatatcga aagccttcgc gaccatggcg ccaccggcgg cctgctgccc
     9301 aaggtgatcg ccgcctacaa tgcaggcccg gtgccggtgg ccgaatggaa cgcccgccgc
     9361 ttcgaccggg gcgatccttt gctctacatc gagtcgctgc cgtactggga gactcgcggc
     9421 tacgtcccga tcgtgcttcg caactactgg atctacgagg aagccgccgg gaagcgcggc
     9481 gacagccggg aagcgctggc ccagggcctg tggccgaaat ttccgggcat gcccggcgcc
     9541 cgcgcggtgc ggctcaagcc ggacgagacg aagctcgcct acgggacgga atagtcctgc
     9601 gccgggttcc cggtacggag actagttctt gatccgttcc agtttcgcgg ctacaccgtc
     9661 gccatgcacc gtcccgacgt ccgccacggc cgcggcgcgc ccgagaaccg gacgccgacc
     9721 cgattcaacc ttcccgtgcg ggaagcggac ggcgactggc tggacgcccg cgaggcggtg
     9781 gacgacgggc cgccgccgct gcggacgacg gtgacggtcg agaagccccg gtcgatcatc
     9841 gcgtggaacc ggtcgcccga catcggcttc gaccgctcga tcaattccta tcgcggctgc
     9901 gagcatggct gcatctactg cttcgcccgc cccacccacg ccttccacga tctgtccccg
     9961 ggcctcgact tcgagaccaa gctgttcgcc aagcccgacg cggcgaagat cctgcgcgag
    10021 gagctggcga agcccggcta cgtcgtcagg ccgatggcga tcggcaccaa taccgacccc
    10081 taccagccga tcgagggcaa gtggcggatc acccgctccg tgctcgaggt gctcgccgaa
    10141 accaggcatc cggtgacgat caccaccaaa tcggaccggg tgctgcgcga tctcgacctg
    10201 ctggcgcgaa tggccgcgga aggcctggcg tcggtggcgc tttcggtgac gtcgctgacg
    10261 ccggcgattt cccggacgct ggagccccgg gccccgtccg gcctcaagcg cctcgcggcg
    10321 gtgaagacgc tggccgaagc gggcgtcccc gcgaccgtgg cgatcgctcc ggtcatcccc
    10381 ggaatcaccg atcatgaggt cgagcatatc gtcgaggcgg cggccgaggc gggcgcggtc
    10441 ggcgccttct tcctgccggt ccggctgccc catgaagtcg cgcccctgtt ccgggcctgg
    10501 ctcgacgagc atttcccgga tcgggccgac aaggtgatga acatcatccg ctcgatgcgc
    10561 ggcgggaagg acaacgaccc gaactggttc acccggatga gcggctcggg gccgtgggcc
    10621 gagctgatgc gcgtgcgctt cgaggtggcg gcgaagaagc tcgggctgaa ccgggacaag
    10681 aggaagcttc gttgcgacct gttcgcgccg cccacgggcc cgcaagggga attgttcggc
    10741 cgaatatagt acgccggcaa agcggccctg cctctcggat cgacagaatt ccgacgttat
    10801 gctagatatt gcaggcggag acacgcaatg cggttcatcc tttcgggaat tccgccggag
    10861 tcgcgggtag atcaaggccc ggcgcagcta ggcgtgagcg taggctccag atttggaaac
    10921 ctcggtgttg cataccaact tgctctcggc gaagtcgtca ctatcgaggg cgcgacggac
    10981 gaccaaagta gcctgactgc aagactgctg atcggcaacg acgaggtcga attctcgatc
    11041 gacaacgtgg atgaggattc ggacgttata atttctccgc tgagcgagaa ggtgtttttc
    11101 gtcaacgtct ggcatcggca acgacccgct ccctgtattg ttcgctgtgc cgacgggacg
    11161 gttggccagc cgtgcgtcac ttgccggaaa ggcaacaggg tcgtcaggat ttgctgctga
    11221 catgggcgat ccggctgtcc gtctgatcaa gatctatgtg cgggacaagg aagccaaccc
    11281 cttgcccgac gcggaaatcc ggctttgggt cgatggggaa gagatcggga aaatcgagaa
    11341 ctccgaaggc aacggaacaa ttcagctgtc gccgcccg
//
LOCUS       DASVQZ010000334         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_936830, whole genome shotgun sequence.
ACCESSION   DASVQZ010000334 DASVQZ010000000
VERSION     DASVQZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936830"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VF547_09505"
     CDS             complement(<1..417)
                     /locus_tag="VF547_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX8263096.1"
                     /translation="MPYPEKGRWALIVHGGAKEMEAGEEEDNRSGVIEAVQAGAAVLE
                     RGGSAVDAVEASIRILEDLPVFNAGRGSALNAAGDIEMCSGLMDGSDLSVGAVGAIRN
                     VRNPVSVAKKLLPEKEILLVGEGAVQFAKEAGMEMVP"
     gene            complement(419..>2014)
                     /locus_tag="VF547_09510"
     CDS             complement(419..>2014)
                     /locus_tag="VF547_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8263097.1"
                     /translation="EEFLARSGLTPDSIPEADQVVPLRATANLSTGGTAVDRTNEIHP
                     DNAEIARRAALIVGLDVCGVDFVCPDITRSVRETGGGVIEVNAAPGLRMHIEPSEGAP
                     RDVAKPIIEMLFPRGRPSRVPIIAITGTNGKSTVGRMTKHILRYTGCTVGLTSTTGVY
                     INDILTHEGDATGPRSARMILRDPTVEVAVLETARGGLLREGLAYEEADIAACLNVTA
                     DHLGLKGIETVEDLASVKQVVVEAVRRGGYSVLNADDPLTVKMARRAGGRIIWFSLCG
                     GAEMSPMLREHIDEGGMAVVREAGPEGGTIVLYEDARRELIMKSGDIPATLHGMAEFN
                     VANSLAAIAIALAHEVPILTIRSAMAQFRSTFEQNPGRLNVHDAHGFRVIVDYAHNAA
                     GLEALGKVVKGLSHRYKRTIGSVSMAGDRRDEDIRELGRIAAGIFDELIFREDPYTRG
                     RPRGEVMNLLKDGALQAGRSEEHLHLIAGEHQSTAAALAMGRPGDLVVITPTDVKGAW
                     QQVNEFKPATVRAAGRASFVAAE"
BASE COUNT          275 a          714 c          694 g          331 t
ORIGIN      
        1 cggcaccatt tccatccccg cctctttcgc gaactgcacc gcgccttccc cgacgagcag
       61 tatctccttc tccggcagaa gcttcttcgc cacgctgacc gggttgcgca cgttgcggat
      121 cgcgccgacc gcgcccaccg acaggtcgct cccgtccatc agtccggagc acatctcgat
      181 gtcgcccgca gcgttcaacg ccgatccgcg gccggcgttg aacaccggca ggtcctcgag
      241 gatccggatc gaggcttcga ccgcatcgac ggcgctgccg ccccgctcaa gcaccgccgc
      301 gccggcctga acggcctcga tgacgccgct gcgattgtcc tcctcctcgc cggcctccat
      361 ctccttggcg ccgccatgga cgatgagggc ccagcggccc ttctcgggat agggcatatt
      421 attcggccgc gacgaaggac gcgcggccgg cggcgcggac ggtggcgggc ttgaactcgt
      481 tgacctgctg ccacgcgccc ttgacgtcgg tcggggtgat cacgacgagg tcgccggggc
      541 ggcccatcgc gagcgccgcc gccgtggatt ggtgctcgcc cgcgatgagg tggagatgct
      601 cctccgagcg gcccgcctgg agcgccccgt ccttgaggag gttcatcacc tcgccgcgcg
      661 gacgcccgcg cgtatagggg tcctcgcgga agatgagctc gtcgaagatc ccggccgcga
      721 tcctgcccag ctccctgatg tcctcgtccc gccggtcgcc cgccatcgag accgagccga
      781 tcgtccgctt gtagcggtgg ctcaggccct tgacgacctt gccgagcgcc tcgagcccgg
      841 cggcgttgtg ggcataatcg acgatcaccc ggaagccgtg ggcgtcgtgg acgttgaggc
      901 gtccgggatt ctgctcgaag gtcgagcgga actgcgccat cgccgagcgg atggtaagga
      961 tcggcacctc gtgcgccagc gcgatggcga tggccgcgag cgagttggcg acgttgaact
     1021 cggccattcc gtgcaaggtc gcggggatgt cgcccgactt catgatcagc tcgcgccggg
     1081 catcctcgta gaggacgatg gtgccgccct ccgggcccgc ctcgcggacc acggccatgc
     1141 cgccctcgtc gatatgctcg cggagcatgg gcgacatctc ggcgccgccg cacagcgaga
     1201 accagatgat ccggccgccg gcgcggcggg ccatcttgac ggtcagcggg tcgtccgcgt
     1261 tgagcaccga atagccgccc cggcgaaccg cctcgaccac cacctgcttg acgctggcga
     1321 ggtcctccac cgtctcgatg cccttgagcc cgagatggtc ggcggtgacg ttgaggcagg
     1381 cggcgatatc ggcctcctcg taggcgaggc cctcgcgcag caggccgccc cgcgccgtct
     1441 cgagcacggc cacctcgacg gtcgggtcgc gcaggatcat ccgcgcgctg cgcgggccgg
     1501 tggcgtcgcc ctcgtgggtc aggatgtcgt tgatgtagac cccggtcgtc gaggtgaggc
     1561 cgacggtgca gccggtgtag cgcaggatgt gcttggtcat ccggccgacc gtcgacttgc
     1621 cgttggtgcc ggtgatcgcg atgatcggca cccgcgacgg ccgtccccgc gggaacagca
     1681 tctcgatgat cggcttggcg acgtcgcggg gcgcgccttc cgacggctcg atgtgcatcc
     1741 tcaggcccgg cgcggcattg acctcgatga ctccgccgcc ggtctctcgg accgagcggg
     1801 tgatgtccgg gcagacgaag tcgactccgc acacgtcgag gccgacgatc agcgcggccc
     1861 gccgggcgat ctcggcattg tccggatgga tctcgttggt tcggtcgacc gcggtgccgc
     1921 cggtcgacag attggcggtt gcgcgcaggg gcacgacctg gtccgcctcg gggatgctgt
     1981 ccggcgtcag gccggagcgg gcgaggaact cctc
//
LOCUS       DASVQZ010000335         5137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_937011, whole genome shotgun sequence.
ACCESSION   DASVQZ010000335 DASVQZ010000000
VERSION     DASVQZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937011"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /gene="phaZ"
                     /locus_tag="VF547_09515"
     CDS             <1..907
                     /gene="phaZ"
                     /locus_tag="VF547_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX8263098.1"
                     /translation="NPRLIEITAMNHRKWSIPFITDWRDAKLVPLDSGRFDLDDYIDY
                     LVEFLEHVGPGAHMLAVCQPSVPCYAAAALMSADRNPARPRTLTLMGGPIDTRKAPTA
                     VNTVATQRPHSWFQQNVIASVPYTYPGAGRQVYPGFLQLAGFMAMNLGDHLTGHWEMF
                     KHLVQGDGESAEASKAFYDEYRSVCDMTAEFYLQTVDVVFQRHLLPKGELRHRGRRVD
                     PAAIRDVALLAIEGERDDISGVGQTKAALDISTRLPKAMKQYHLAKGVGHYGIFNGSK
                     WRERIAPVVEDWIAVHDRARGERQA"
     gene            complement(898..1353)
                     /locus_tag="VF547_09520"
     CDS             complement(898..1353)
                     /locus_tag="VF547_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263099.1"
                     /translation="MGLEVIDGTVESAALKRSTAKVAMYERIVIRAADGSERRLEKVA
                     VSPSVSGMLEPGTLGRFYGYKAIDHRGLFAARTRDGRSGFAIPTGNEKLMLMMAIVGL
                     GGFAIMLVFGSKIGLLSLILGVLGVIGYLRYRRDRVEARARYDADAGYA"
     gene            1476..1814
                     /locus_tag="VF547_09525"
     CDS             1476..1814
                     /locus_tag="VF547_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8263100.1"
                     /translation="MKKIEAIIKPFKLDEVKEALHDVGVSGITVTEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEVVVEDGLAERVVEAIANAARTGRIGDGKIFVMEVEEALRI
                     RTGERGSDAI"
     gene            1942..2151
                     /locus_tag="VF547_09530"
     CDS             1942..2151
                     /locus_tag="VF547_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8263101.1"
                     /translation="MDDPRLASMSPEELRSAMRTLGYRTQNDLAAAIGVSRSAVSLWL
                     EGKVGVPRPVAMLLRMLIAAQRRAF"
     gene            2335..3747
                     /gene="glnA"
                     /locus_tag="VF547_09535"
     CDS             2335..3747
                     /gene="glnA"
                     /locus_tag="VF547_09535"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX8263102.1"
                     /translation="MASDAGKILKRIEDEEIEWVDLRFTDPRGKWQHLSMVAGVVDED
                     QLVDGFMFDGSSIAGWKAINESDMILKPDLDAVYLDPFSATPMLVIFCDIVYPGTGEI
                     YARDPRSTARRAENYLKGTGIGDTVFVGPEAEFFMFDDVRFEDGYSASGFRIDDIELP
                     TNSGRDYEGGNLAHRPRAKGGYFPVAPVDSAMDIRGEMVSTMIEMGLPMDKHHHEVAA
                     AQHELGLTYGTLTQTADRMQIYKYVVHMVAHAYGKTATFMPKPIKSDNGSGMHTHMSI
                     WNGGKPLFAGNGYAGLSETALYFIGGVIKHAKALNAFTNPTTNSYKRLVPGFEAPVLL
                     AYSSRNRSASCRIPYGAGEKAKRVEFRFPDALANPYLAYSAILMAGLDGIQNRLHPGD
                     PMDKNLYDLPPQELVNVPTVCGSLREALIALQNDRAFLTKGDVFSDDQIDAYMELKWE
                     EQMRWETTPSPVEYDLYYSS"
     gene            complement(3907..>5137)
                     /locus_tag="VF547_09540"
     CDS             complement(3907..>5137)
                     /locus_tag="VF547_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8263103.1"
                     /translation="AALYAAKRDGRGTFRFFAPEMHALATERQILEKDLRHALSQDQL
                     SLLYQPIVDSVSEEVVAFEALLRWHHPVRGLLPPSLVIPLAEESGLMPRIGDWVLRTA
                     CAEAAKWPRHIRVAVNLSPVQFADPALAATVTGTLAASRLDPERLELEITEAVFLADR
                     AVTEATLARLRGIGVRLGLDNFGTGHSGLGHLRDAPLDKIKIDQSFVRGAADPGSRNG
                     AIVRAIVVLAESLGMDTTAEGAETIEELALIRRLGCSQVQGFIFGRPMAADEALNLAV
                     ESRPSAEVIGFSRPPRHRLIRNGRLNWEGRAVEVRLRNISTGGAMIECDRALAPGTAV
                     ELDLDEAGKLDAEVRWCHRGQVGLAFAQPFALGKLARKRRRSGPPDMLMPQYLGPQPE
                     AAETAASPLATKKRRAG"
BASE COUNT          865 a         1769 c         1725 g          778 t
ORIGIN      
        1 gaatcccaga ttaattgaga tcaccgcgat gaaccacagg aagtggagca tccccttcat
       61 caccgactgg cgcgacgcca agctcgtccc gctcgactcg gggcgcttcg acctcgacga
      121 ttatatcgac tatctggtcg agttcctcga gcatgtcggc cccggcgccc atatgctcgc
      181 ggtctgccag ccctcggtgc cctgctacgc cgcggcggcg ctgatgagcg ccgaccgcaa
      241 cccggcgcgg ccgcggaccc tgaccctgat gggcgggccg atcgacacga ggaaggctcc
      301 gaccgcggtc aacaccgtcg ccacccagcg cccgcacagc tggttccagc agaacgtcat
      361 cgcctcggtg ccctacacct atccgggcgc cggccggcag gtctatcccg gcttcctcca
      421 gctcgccggc tttatggcga tgaacctggg cgaccacctc accggccatt gggagatgtt
      481 caagcatctc gtccagggcg acggcgagag cgccgaggcg agcaaggcct tctacgacga
      541 atatcgctcg gtctgcgaca tgaccgccga attctacctt cagacggtcg acgtcgtctt
      601 ccagcgccat ctgcttccga agggcgagct gaggcaccgc ggccgccgcg tcgatccggc
      661 ggcgatccgc gacgtcgccc tcctcgccat cgagggcgag cgcgacgaca tctccggcgt
      721 cgggcagacc aaggcggcgc tcgacatctc gacccggctg cccaaggcga tgaagcaata
      781 tcatctcgcc aagggcgtcg gccattacgg catcttcaac ggcagcaaat ggcgcgagcg
      841 catcgccccc gtcgtcgagg actggatcgc ggtgcacgac cgagcgcgcg gcgagcgtca
      901 ggcgtagccg gcgtcggcgt cgtagcgggc gcgggcctcg acgcggtcgc ggcggtagcg
      961 caagtagccg atcacgccga gcactccgag gatcaggctc aagagcccga tcttgcttcc
     1021 gaagacgagc atgatcgcga agccgcccag gccgacgatc gccatcatca gcatcagctt
     1081 ctcgttgccg gtcgggatgg cgaagcccga gcggccgtcg cgggtgcggg cggcgaacag
     1141 gccgcgatgg tcgatcgcct tgtagccgta gaagcggccc agcgtgcccg gctcgagcat
     1201 ccccgacacg ctcggcgaga cggcaacctt ctccagccgc cgctccgatc cgtcggcggc
     1261 gcggatcacg atccgctcgt acatcgcgac cttggcggtc gagcgcttca gagcggcgga
     1321 ttcgaccgtc ccgtcgatca cttccagtcc cataagccgt ctccccctgg cgtgccggtc
     1381 cttgtccacc gccgcggagg gtttggcaat gcggacatct tgcgcgttcg cagcggcggg
     1441 ctatgctgcg gcgcagcagg tgcaggagtc ggccggtgaa gaagatcgag gcgatcatca
     1501 agccgttcaa gctcgacgag gtcaaggaag cgctgcacga cgtcggggtc tccggcatca
     1561 ccgtcaccga agcgaagggg ttcggccgcc agaagggcca taccgagctc taccggggcg
     1621 ccgaatatgt cgtcgacttc cttcccaagg tgaagctcga ggtggtcgtc gaggacggcc
     1681 tcgccgagcg ggtggtcgag gcgatcgcca acgcggcgcg caccggccgg atcggcgacg
     1741 gcaagatctt cgtcatggag gtcgaggagg ctttgcgcat ccggaccggc gagcggggct
     1801 cggatgcgat ctgagaaccg ctcatcctga gctgggaccg agcctggcga aggcccgtgt
     1861 cgaagggccg cccttcgata cgccgcttcg acttcgccca gcggctactc aggatgagcg
     1921 ggaagggcta aggcccgacc cgtggacgat ccccgcctcg cctccatgtc gcccgaggag
     1981 ctgcgctcgg cgatgcgcac gctcggctac cgcacccaga acgacctcgc cgccgcgatc
     2041 ggggtaagcc gctcggcggt cagcctgtgg ctcgagggca aggtcggggt gccgaggccg
     2101 gtggcgatgc tgctgaggat gctgatcgcg gcgcagcgaa gggcgttctg atcttcccca
     2161 cgtgaaatgt tcacgcccgg ctggaccggg cgcaagggtg cgggaaagct cgctccatac
     2221 cgcttcggat tcgccgctcg tcatgccagc gcaggctggc atccatgcgg aggcgttgcc
     2281 ggcggatggg cagaatggac cccggccttc gccggggcga cgaaaggacc gaccatggca
     2341 agcgacgcgg gcaagattct gaagcggatc gaggatgagg agatcgagtg ggtcgacctg
     2401 cggttcaccg acccgcgcgg caagtggcag catctctcca tggtcgcggg cgtggtcgac
     2461 gaggaccagc tggtcgacgg cttcatgttc gacggcagct cgatcgccgg ctggaaggcg
     2521 atcaacgaat ccgacatgat cctgaagccc gacctcgacg ccgtctatct cgacccgttc
     2581 agcgcgacgc cgatgctggt catcttctgc gacatcgtct atccgggcac cggcgagatc
     2641 tacgcccgcg acccccgctc gacggcgcgc cgggccgaga actatctcaa gggcacgggc
     2701 atcggcgaca ccgtcttcgt cggccccgag gccgaattct tcatgttcga cgacgtccgc
     2761 ttcgaggacg gctacagcgc gtcgggcttc cgcatcgacg acatcgagct gccgaccaac
     2821 tcggggcgcg actatgaggg cggcaatctc gcccaccggc cgcgcgccaa gggcggctat
     2881 ttcccggtgg cgccggtcga cagcgccatg gacatacgcg gcgagatggt ctcgacgatg
     2941 atcgagatgg gcctgccgat ggacaagcac catcacgagg tcgctgcggc ccagcacgaa
     3001 ttgggcctca cctacggcac cctcacccag accgccgacc ggatgcagat ctacaaatat
     3061 gtcgtgcaca tggtcgccca cgcctacggc aagaccgcga ccttcatgcc caagccgatc
     3121 aagtcggaca acggctcggg catgcacacc cacatgtcga tctggaacgg cggcaagccg
     3181 ctgttcgccg gcaacggcta tgccgggctc agcgagacgg cgctctactt catcggcggg
     3241 gtgatcaagc acgccaaggc gctcaacgcc ttcaccaacc cgaccaccaa cagctacaag
     3301 cgcctggtcc ccggcttcga ggcgccggtc ctgctcgcct attcgagccg caaccgctcg
     3361 gcctcgtgcc gaatccccta cggcgccggc gagaaggcca aaagggtcga gttccgcttc
     3421 cccgacgcgc tcgccaaccc ctatctggct tattcggcga tcctgatggc gggcctcgac
     3481 gggatccaga accgcctcca ccccggcgat ccgatggaca agaacctcta cgacctcccg
     3541 ccgcaggagc tggtcaacgt cccgaccgtc tgcggcagct tgcgcgaggc tctgatcgcg
     3601 ctccagaacg accgcgcctt cctgaccaag ggcgacgtgt tcagcgacga ccagatcgac
     3661 gcctatatgg aattgaagtg ggaggagcag atgcgctggg agaccactcc cagcccggtc
     3721 gaatacgacc tctattattc gagctgatct ctccggaaat tcctcccccg gatttccggg
     3781 ggagggggac cgcgcgaagc gcggtggagg ggttcttcag agccggtgag gcccctccac
     3841 catgcttcgc atggtccccc tcccctgcaa aatgcagggg aggaattaaa gggggtcgag
     3901 ggcagatcag cccgcccgcc gcttcttcgt cgcgaggggc gaggcggcgg tttccgcggc
     3961 ctccggctgc ggcccgagat attgcggcat cagcatgtcg ggcgggccgg accgccgccg
     4021 cttgcgggcc agcttgccga gcgcgaaggg ctgagcgaag gcgagtccga cctggccacg
     4081 gtggcaccag cggacctcgg cgtcgagctt gccggcctcg tcgaggtcga gctcgacggc
     4141 ggtgcccggc gccagcgccc ggtcgcactc gatcatcgcc ccgccggtgg agatgttgcg
     4201 cagccgcacc tcgaccgccc ggccctccca gttcagccgg ccgttgcgga tcaggcggtg
     4261 gcggggcggg cggctgaagc cgatcacctc ggcgctcggc cggctctcga cggcgagatt
     4321 cagcgcctcg tccgccgcca tcggcctgcc gaagatgaag ccctgcacct gcgagcagcc
     4381 gagccggcgg atcagcgcca gctcctcgat cgtttccgcg ccctcggcgg tggtgtccat
     4441 gcccaggctc tcggcgagga cgacgatcgc ccggacgatg gcgccgttgc ggctgcccgg
     4501 atcggcggcg ccgcggacga agctctggtc gatcttgatc ttgtcgagcg gcgcgtcgcg
     4561 cagatggccg agccccgaat ggccggttcc gaaattgtcg aggccgagcc gaacgccgat
     4621 gccgcgaagc cgcgccaggg tcgcctcggt gaccgcccgg tcggccagga acacggcctc
     4681 ggtgatctcg agctcgagcc gttccgggtc gagccgcgag gccgccagcg tgccggtgac
     4741 ggtcgccgcc agcgccggat cggcgaactg gaccggcgac aggttgaccg cgacgcggat
     4801 gtggcgcggc catttcgcgg cctcggcgca ggcggtcctc agcacccagt cgccgatccg
     4861 cggcatcagg ccgctttcct cggcgagcgg gatgaccagc gacggcggca gcagcccgcg
     4921 caccgggtgg tgccaccgca gcagggcctc gaaggcgacc acctcctcgg acacggaatc
     4981 gacgatcggc tggtagagca ggctgagctg gtcctgcgag agcgcgtggc gaaggtcctt
     5041 ctcgaggatc tggcgctcgg tcgccagcgc atgcatttcc ggggcgaaga agcggaaggt
     5101 gccgcggccg tcgcgcttgg cggcgtagag cgcggcg
//
LOCUS       DASVQZ010000336         6187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_69814, whole genome shotgun sequence.
ACCESSION   DASVQZ010000336 DASVQZ010000000
VERSION     DASVQZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69814"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..426
                     /locus_tag="VF547_09545"
     CDS             7..426
                     /locus_tag="VF547_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX8263104.1"
                     /translation="MRQATGTLEFQTSRQGLVEITREVSAWTSAQGITDGLLTLFCRH
                     TSASLLIQENAAPEVRGDIEAWVARLAPEDPAAYAHRDEGPDDMPAHLRTILTQVQLA
                     IPLTGGRLALGTWQGIYLFEHRRRPHRRGLALHLIGE"
     gene            431..910
                     /locus_tag="VF547_09550"
     CDS             431..910
                     /locus_tag="VF547_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX8263105.1"
                     /translation="MSLESVRAWLAEHAPDLPLIEVAETTATVETAAQALGVEPGRIA
                     KTLAVRVRDSVFLLVARGDARLDNAKTKAEFHARPRMLGAEETLALTGHPVGGVCPFG
                     LATKLPVYCDLSLREFDLVYPAAGSLNSSVEVAPERLFDLVGDRWVDLCRRPEAAAD"
     gene            943..1566
                     /locus_tag="VF547_09555"
     CDS             943..1566
                     /locus_tag="VF547_09555"
                     /inference="COORDINATES: protein motif:HMM:NF027558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEX8263106.1"
                     /translation="MKRLPLLLAPLLLAACASDGTGPAGAVPAAESWRSVATEFDRER
                     ARKWRTAWVRALASARASGHAEAIAREGSLLEPDAALKGAAPPPGDYRCRVLKIGAQR
                     QGLLDYVAYPQFDCRIAAGAGAMDFVKLTGSQRPVGRLFADGDRRLVFLGTLQLGDER
                     GTLRYGHDRQRDMIGLLERVGERRWRLAFPYPAFESLLDVVELLPKE"
     gene            1751..3058
                     /locus_tag="VF547_09560"
     CDS             1751..3058
                     /locus_tag="VF547_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8263107.1"
                     /translation="MRYLALPLIALAAPAAADPLADQVRQDMPSLMTLYRELHANPEL
                     SMQEVKTSAGLAAEARKLGFEVTTGVGKTGVVAVMRNGPGPVLLMRADMDALPVVEQT
                     GLPFASKVKAMSAAGTESGVMHACGHDTHMTGWLGTARRMAAAKAQWSGTLVMILQPA
                     EETGEGARAMLDDGLFSRFPKPTHALAWHDSASLPAGVLGITVGPAFANVDTVDIQVK
                     GVGGHGAVPQNTRDPIVLASRIVGTLQTLVSRELDPQDAAVVTVGSFQAGTKHNIISD
                     QATLLLTVRSYSEKTRRALLDGIRRIARGEAIAAGMPEDRMPVVTVREGEFTPAAVNT
                     EKLSADTAVLFSRRFGAERVRAVPPPMVGEDFGRYHLADKNIESLIFWVGGVPKSKWD
                     AAGGDTSKLPSLHSPYWAPEAETVIATGIEAMVTAAMGVLGKR"
     gene            complement(3195..4559)
                     /gene="gdhA"
                     /locus_tag="VF547_09565"
     CDS             complement(3195..4559)
                     /gene="gdhA"
                     /locus_tag="VF547_09565"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX8263108.1"
                     /translation="MNRRGDSGHVDLEGFMAGVKRRNPHQPEFVQAVQEVAEDIFEFT
                     EAKPEYHEAQILRRMAEPDRIVSFRVCWEDDKGNIRVQRGYRVQNNNAIGPYKGGIRF
                     HPSVNESILKFLAFEQTFKNALTGLPMGGGKGGANFNPKGKSVNEIMRFCQSFMTELF
                     RHIGANVDVPAGDIGVGAREIGYMFGQFKRITNTWEGVLTGKGLEYGGSLIRTEATGY
                     GAVYFLANMLATRGQDLVGKRVVISGSGNVATHAAEKVVQLGGKVLTFSDSEGFIHDP
                     QGITQEKIEWVKAHKTKRRGRICEYAEEFKGATFHAGGRPWAVECDAALPCATQNELL
                     GDDARALVRNGCIAVSEGANMPTDLEGVRVFKQARLLYAPGKAANAGGVAVSGLEMSQ
                     NSARISWKEEELQRLLKDIMDGIHERCLEHGRVSDAYVDYVKGANIAGFKKVADAMLA
                     FGVV"
     gene            complement(4764..5426)
                     /locus_tag="VF547_09570"
     CDS             complement(4764..5426)
                     /locus_tag="VF547_09570"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX8263109.1"
                     /translation="MNIILLGPPGAGKGTQAARLIAERGLVQLSTGDMLREAIKAGSP
                     IGLRAKAIMEAGELVSDDIVDELIANRLDLPERYAGSGFIFDGYPRTEAQAISLDRLL
                     AERELRLDHVIELEVDAEALVDRITGRFSCAKCGEGYHDRYKLPRIATLCDVCGADQF
                     KRRPDDNEETVRTRLAEYLAKTAPILPYYEARGLVRRVDGMAPIKEVERAIETILDGT
                     VV"
     gene            complement(5423..>6187)
                     /locus_tag="VF547_09575"
     CDS             complement(5423..>6187)
                     /locus_tag="VF547_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX8263110.1"
                     /translation="AQLPTALAQAFEGGRTGTLSPLAIMGVIALAAGLILFICFMERA
                     QRRVLIQYPKRQTARGQMQAEKSHLPLKINTSGVIPPIFASSLLLMPLTIAQFAGNRA
                     VDTESTWNNILLTVTNALQHGAPLYMALYAAGIIFFCFFYTAVVFNPEETAENLKRYG
                     GFIPGIRPGKRTEEYLDYVLTRITVIGAAYLTVICLVPEFLIARAGIPFYLGGTSLLI
                     VVNVTMDTVTQIQSHLIAHQYGDLIKKAKLKGGSRR"
BASE COUNT          929 a         2122 c         2122 g         1014 t
ORIGIN      
        1 gcctcgatgc gacaggcgac cggcaccctc gaattccaga cctcgcgcca ggggctggtc
       61 gagatcaccc gggaagtttc cgcctggacc tccgcccagg gcataaccga cggcctgctg
      121 accctcttct gccgccacac ctcggcctcg ctcctcatcc aggagaatgc ggcgcccgag
      181 gtccgcggcg acatcgaggc gtgggtggcg cggctggcgc ccgaggatcc cgccgcctac
      241 gcccatcgcg acgaggggcc ggacgacatg ccggcacatt tgaggacgat cctcacccag
      301 gtccagctcg ccatcccgct gaccggcggc cggctcgcgc tcggcacctg gcagggcatc
      361 tatctgttcg agcaccgccg gcggccgcac cggcgcggcc ttgcgctgca cctgatcgga
      421 gaatgacgcc ttgagcctcg aatcggttcg agcctggctg gccgagcacg ccccggacct
      481 tccgctgatc gaggtggccg agacgacggc gacggtcgag accgcggccc aggcgctggg
      541 cgtcgagccg ggccggatcg ccaagacgct ggccgtccgg gtgcgcgatt ccgtcttcct
      601 gctcgtcgcg cgcggcgacg cccggctcga caatgccaag accaaggccg aattccacgc
      661 ccggccgcga atgctgggcg cggaggagac cctggccctg accggccatc ccgtcggcgg
      721 agtctgcccg ttcggcctgg cgacgaagct tcccgtctat tgcgacctct cgctcaggga
      781 attcgacctc gtctatccgg ccgccggctc gctcaacagc tcggtcgagg ttgccccgga
      841 gcgcctgttc gaccttgtcg gcgatcgctg ggtcgacctt tgccggcggc ccgaggcggc
      901 cgcggattga tcggaggcgg tccgcgctcc tatggtcgga cgatgaagcg cctccccttg
      961 ctgctggctc cgctcctcct cgccgcctgc gcctccgacg ggaccggccc ggccggggcg
     1021 gtgcccgcgg ccgagagctg gcggtcggtc gcgaccgagt tcgaccgcga gcgggcgcgc
     1081 aaatggcgaa ccgcctgggt gcgggcgctc gccagcgcgc gcgcctccgg ccacgccgag
     1141 gcgatcgcgc gggaggggtc gctgctcgag cccgacgcgg cgctcaaggg cgcggccccg
     1201 ccgccgggcg actatcgctg ccgggtgctg aagatcggcg cccagcgcca gggcctgctc
     1261 gattatgtcg cctatccgca gttcgactgc cggatcgccg ccggcgcggg cgcgatggac
     1321 ttcgtcaagc tgaccggatc gcagcggccg gtcggccggc tgttcgccga cggcgaccgc
     1381 cgcctcgtct tcctcggcac cctccagctc ggcgacgagc gcgggaccct gcgctacggc
     1441 cacgaccggc agcgcgacat gatcggactg ctcgagcggg tcggcgagcg gcgctggcgg
     1501 ctcgccttcc cctatcccgc cttcgaatcc ctgctcgacg tggtcgagct cctgcccaag
     1561 gaatgatgcg atcctaaaat cctcccccgg ctttccgggg gagggggacc gcgcgaagcg
     1621 cggcggaggg gttcttccag tccagagagt ggcccctcca ccatgcttcg catggccccc
     1681 ctcccctgca gatgcagggg aggatttttt agtgcctcga cccgcctgtg aaaccggcta
     1741 aaccaaggcc atgcgttacc tggcccttcc ccttatcgcc ctcgcggctc ccgccgccgc
     1801 cgacccgctc gccgaccagg ttcggcagga catgccgtcg ctgatgactc tctatcgcga
     1861 gctccacgcc aatcccgaac tgtcgatgca ggaggtgaag acctcggccg ggctcgccgc
     1921 ggaggcgcgt aagctcggct tcgaggtgac cacgggcgtc ggcaagacgg gcgtcgtcgc
     1981 ggtgatgagg aacgggcccg ggccggtgct gctgatgcgc gccgacatgg acgccctgcc
     2041 ggtggtcgag cagaccggcc tgcccttcgc cagcaaggtc aaggcgatga gcgccgccgg
     2101 caccgagagc ggggtgatgc acgcctgcgg ccatgacacc cacatgaccg gctggctcgg
     2161 cacggcccgg cggatggcgg cggcgaaggc gcaatggtcc ggaaccctgg tgatgatcct
     2221 ccagcccgcc gaggagaccg gcgagggcgc cagggcgatg ctcgacgacg gcctgttcag
     2281 ccgcttcccc aagccgaccc acgccttggc ctggcacgac agcgcctcgc ttccggccgg
     2341 agtgctcggc atcaccgtcg gacccgcctt cgccaatgtc gacacggtcg acatccaggt
     2401 gaagggcgtc ggcggccacg gcgcggtgcc gcagaacacc cgcgacccga tcgtgctggc
     2461 gagccggatc gtcggcacgc tgcagaccct ggtcagccgc gagctcgacc cgcaggacgc
     2521 ggcggtggtg acggtcggca gcttccaggc cggcaccaag cacaacatca tttcggacca
     2581 ggcgacgctg ctgctcaccg tcaggagcta cagcgagaag acccgccggg ccctgctcga
     2641 cggcatcagg cggatcgcgc gcggcgaggc gatcgcggcc ggaatgccgg aggaccggat
     2701 gccggtggtg acggtccgcg agggcgagtt caccccggcg gcggtcaaca ccgagaagct
     2761 gagcgcggac accgccgtcc tgttcagccg tcgcttcggg gctgagcggg tgcgggccgt
     2821 gccgccgccg atggtcggcg aggatttcgg ccgctaccac ctcgccgaca agaatatcga
     2881 aagcctgatc ttctgggtcg gaggcgtgcc gaagtcgaaa tgggacgccg ccggcggcga
     2941 cacctcgaag ctgccctcgc tccacagtcc ctattgggcg cccgaggccg agaccgtgat
     3001 cgccaccggg atcgaggcga tggtgacggc cgcgatgggc gtgctgggga agcggtgacg
     3061 aattcctccc ctgcatttgc aggggagggg gaccgtccga aggacggtgg agtggttctt
     3121 cggagtcacc gggaaaaacc ccttcaccgc gcttcgcgcg gtccccctcc ccgacgaagg
     3181 ccggggagga attttcacac cacgccgaag gccagcatcg cgtcggcgac cttcttgaag
     3241 ccggcgatgt tggcgccctt cacatagtcg acataggcgt ccgagacccg gccatgctcg
     3301 aggcagcgct cgtggatgcc gtccatgatg tccttgagca ggcgctgcag ctcctcctcc
     3361 ttccagctga tccgggcgct gttctggctc atctcgagcc ccgagacggc gactccgccg
     3421 gcattggcgg ccttgcccgg ggcgtagagc agccgggcct gcttgaacac gcgcacgccc
     3481 tcgaggtcgg tcggcatgtt ggcgccctcc gagacggcga tgcagccgtt cctgaccaag
     3541 gcgcgggcgt cgtcgccgag cagctcgttc tgcgtcgcgc aggggagcgc ggcgtcgcat
     3601 tcgaccgccc agggacggcc tccggcatgg aacgtcgccc ccttgaactc ctcggcatat
     3661 tcgcagatcc ggccgcggcg cttggtcttg tgggccttga cccactcgat cttctcctgg
     3721 gtgatgccct gcgggtcgtg gatgaagccc tcgctgtcag agaaggtcag caccttgccg
     3781 ccgagctgga cgaccttctc ggcggcgtgg gtggcgacgt tgcccgagcc cgagatgacg
     3841 acccgcttgc cgaccaggtc ctgcccccgg gtggccagca tgttggcgag gaaatagacc
     3901 gcgccgtagc cggtggcctc ggttcggatc agcgagccgc catattcgag gcccttgccg
     3961 gtcagcaccc cttcccaggt gttggtgatg cgcttgaact ggccgaacat gtagccgatc
     4021 tcgcgggcgc cgacgccgat gtcgcccgcc ggcacgtcga cattggcgcc gatgtgccgg
     4081 aacagctcgg tcatgaagct ctggcagaag cgcatgatct cgttcacgct cttgcccttg
     4141 ggattgaagt tcgcgccgcc cttgccgccg cccatcggca ggccggtcag ggcgttcttg
     4201 aaggtctgct cgaaggcgag gaacttcagg atcgattcgt tgaccgacgg gtggaagcgg
     4261 atgccgccct tgtacggtcc gatcgcattg ttgttctgga cccggtagcc gcgctggacg
     4321 cggatgttgc ccttgtcgtc ctcccagcac acgcggaacg agacgatccg atccggctcc
     4381 gccatccggc gcaggatctg ggcctcgtga tattcgggct tggcttcggt gaactcgaat
     4441 atgtcctcgg cgacctcctg gaccgcctgg acgaactcgg gctggtgcgg attgcgccgt
     4501 ttgacgccgg ccatgaagcc ttcgaggtcg acatgccccg agtcgcctcg tcggttcatc
     4561 cgctcgctcc cggtcccggc gccccgttcg gccggagtcg ccgcgcgtgg atagcatgat
     4621 tcggcgggaa tcattcctcc ccgcgatttc gcggggaggg ggacggcgcg aagcgccgtg
     4681 gaggggttct tcccactccg aaaaggaccc ctccaccagg cttcgccggg tccccctccc
     4741 ccagccgatg ccggggagga attttatacg accgttccgt ccaatatcgt ctcgatcgcc
     4801 cgctcgacct ccttgatcgg agccatgccg tcgacccgcc ggacgaggcc gcgggcttcg
     4861 taatagggca ggatgggcgc agtcttggcg agatattcgg cgaggcgggt tcggaccgtc
     4921 tcctcattgt cgtcgggccg gcgcttgaac tggtcggcgc cgcaaacgtc gcaaagagtc
     4981 gcgattcgcg gcaatttgta gcggtcgtga tacccttcgc cgcatttggc gcaggagaag
     5041 cggccggtga tccggtcgac gagcgcctcg gcgtccacct cgagctcgat gacgtggtcg
     5101 agcctcaatt cccgctcggc gagcagccgg tcgagcgaga ttgcctgcgc ctcggtgcgc
     5161 ggatagccgt cgaagatgaa gcccgagccg gcatagcgct cgggcaggtc gaggcggttg
     5221 gcgatcagct cgtcgacgat gtcgtccgac accagctcgc ccgcctccat gatcgctttg
     5281 gccctgagcc cgatcgggct tccggccttg atcgcctcgc gaagcatgtc cccggtcgag
     5341 agctggacga ggccccgctc cgcgatcagc cgagccgcct gggttccctt gcctgccccg
     5401 ggagggccga gcaggatgat gttcacctgc gcgagccccc cttgagcttc gccttcttga
     5461 tcaggtcgcc atattgatgg gcgatgaggt ggctctggat ctgcgtgacc gtgtccatgg
     5521 tcacgttgac gacgatcagg aggctggtgc cgccgagata gaaggggatt ccggcccggg
     5581 cgatcaggaa ctccggcacc aggcagatga ccgtcagata ggcggcgccg atgacggtga
     5641 tccgggtgag gacgtagtcg agatattcct cggtgcgctt gccggggcgg atgccgggga
     5701 tgaagccgcc gtagcgcttc aaattctccg ccgtctcctc cgggttgaac accaccgccg
     5761 tgtagaagaa gcagaagaag atgatgccgg ccgcatagag cgccatgtag agcggggcgc
     5821 cgtgctggag ggcgttggtc accgtcagca ggatgttgtt ccaggtgctc tccgtgtcga
     5881 ccgcgcggtt gccggcgaac tgggcgatgg tgagcggcat cagcagcagc gacgaggcga
     5941 agatcggcgg gatgacgccc gacgtgttga tcttcagcgg caaatgcgac ttctcggcct
     6001 gcatctggcc ccgcgccgtc tgccgcttgg gatattggat gagcacccgc cgctgcgccc
     6061 gctccatgaa gcagatgaag aggatgaggc cggcggcgag cgcgatcaca cccatgatcg
     6121 cgagcggcga cagcgtgccg gtgcggccgc cttcgaacgc ctgggcgagc gcggtcggga
     6181 gctgcgc
//
LOCUS       DASVQZ010000337         8759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_69918, whole genome shotgun sequence.
ACCESSION   DASVQZ010000337 DASVQZ010000000
VERSION     DASVQZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69918"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..93)
                     /locus_tag="VF547_09580"
     tRNA            complement(1..93)
                     /locus_tag="VF547_09580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Ser,seq:gct)
     gene            237..1619
                     /locus_tag="VF547_09585"
     CDS             237..1619
                     /locus_tag="VF547_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263111.1"
                     /translation="MSPNDQLSILSGAGLLITFSGVLISQLTRVSVDGEGNRKVLTPT
                     GRLAIAISVIGLFGAVASEALRASIRWQSEKAAAVKAAVEEQRRLDDEAWKTRSEQLA
                     TAIFGNTETQLDRTERNLREMLAGFSANQESIFRTQLAVVEARQRVLADNLLREARLY
                     GRLSAASSPLFRLTLELEIGRAPPELLAAVRHGLRHSRDFFKDSSYIDLNEHHNIDSR
                     DDMAMARRELYRQAVQPVLSWIANDSIQSAQSVMIVSLDDQFSAVSAVGWAHGPPLGD
                     EDNSALFNYEKDFSWVPSGLLGGPLEWNAALGAGDQSHYPAVDVRVAGSSIFLRADVS
                     PAALDRAITRYAKASATTATLPDQLFVLFWSPVPPGETKPFDPPKLPLDSKGVHDVAR
                     YVASGGRTRSSSPPGTWGRSLRIRLIPNGVEAIAKTYRLRPTGDGELFDGIRDDEYGG
                     YLRAWRGSAR"
     gene            1695..2954
                     /gene="serS"
                     /locus_tag="VF547_09590"
     CDS             1695..2954
                     /gene="serS"
                     /locus_tag="VF547_09590"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX8263112.1"
                     /translation="MLSLQFIRDHPDTVRQAAEAKNAAVDLDRLLALDGEVRSLKTRI
                     DELRRQRNEISASFKTADPAERPALGAKAKAFGAEAADSEAALADRSAELEALMLRVP
                     NIPWEGAPVGPDESFNKVVRTEGEPPRFDFEPRDHVALAEMNDWADLSRITQVAGSRM
                     YALKGRLALLEQALMFWAQQIIAADGFTLMTVPALAKPQAFVATGHFPGHEEEAYEVA
                     KDGLYLAGTAEIALTGLHSGEILEAAQLPILYAGYSPCFRREAGSAGRDVRGLLRVHQ
                     FYKLEQYVICADDESESQRWHERLLANAERLLQGLEIPYQVVETSTGDMGLGKFRMND
                     IESWVPSLGKYRETHSCSTLHDWQARRANIRWRDAERKVRFVHTLNNTALASPRILVP
                     LLENHQQADGRVRLPAALRGLMGGDYL"
     gene            2938..3630
                     /locus_tag="VF547_09595"
     CDS             2938..3630
                     /locus_tag="VF547_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8263113.1"
                     /translation="MATISDTERPPLRLLMREGAMIASRVWRFFGRVGERGPADGPPL
                     MVIPGFLASDRSTLGLQRALARAGFRATGWGLGINRGVRPDTLDKIAAEVERHGRGRP
                     VVLVGWSLGGVFAREVAKRRPDLVEKVVTLGAPFSGDPRANNAWRLYELIAGHRVDAP
                     PIDARPGEKPPVPTLALWSRRDGVVAPACARGRDDESDHRVELDCSHMGFGVSSRAFP
                     KIVSAIIAFPGT"
     gene            3681..5330
                     /locus_tag="VF547_09600"
     CDS             3681..5330
                     /locus_tag="VF547_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263114.1"
                     /translation="MRYILAPEWSHLAAATAAAFVLGYLLCWIMDRLFLKELVDDRIA
                     GIALSCCLAFVVIMAWGTIVLTGLAWSDPIIYGRPIVIPPFPYAVSLILGLAGVGILR
                     TILHGREYAEGEEELVFDEDLYDQAQYDEEVLAFDERHAHKDYFRRHWAGHLLLPVTF
                     WVNFVLVAVLIAAAVEYVGNRLRDNGGTLRGLAIIALAYGVFSIIFWAWSSVGIWRSA
                     YWHRRRGGTPGWGLAARILVVVGGLVILVRSGDIALRTRELGTLAAGGDVIGPVAEMK
                     VSGDGRDLSVRGSLAAGSAERFEALLAANPDVRRVVLHSPQGRYFEAERMAALVRSRG
                     LDTRVDDVCLSPCTDLLVAGRERTAPEQARIGFHQPSFPADDGYGMRKAVERLRADYL
                     AAGVKREFVDRVLATPPPTLWFPDPVELVEANLLTGSDVFVTKNGARRRENAVEMRLR
                     RNIAATAERINATLPRRLSADTTLLRVSASGTTLTHHYRIDAEKIDAAGTRIGLGQQL
                     RRQMCADVDMALAIREGARFVHSYSDRRGRHAVDIAVTQCP"
     gene            complement(5314..5717)
                     /locus_tag="VF547_09605"
                     /pseudo
     CDS             complement(5314..5717)
                     /locus_tag="VF547_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            complement(5714..7774)
                     /locus_tag="VF547_09610"
     CDS             complement(5714..7774)
                     /locus_tag="VF547_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8263115.1"
                     /translation="MTDPLPAPPRAEPRPFSYERHGYRVEDPYAWLKDPGYPSVEDED
                     VLAYLKAENAYFEAAMKPHAALVETLFEEMKGRLKEDESSVPVRDGDWLYWWAFEPGA
                     QYRTWYRRRAAGGAEQVVFDEPAEAEGKEYFRLGALEVSPDGRLAAILVDDSGSERFD
                     LRIRDLATGEDLETVTDVAIGHPVWSGDGEGIVFTEVNEHWRSYRARYHRLGSPASDD
                     VTLYEETEALGFSVGVSRSQDRSLIFIATGDNATSEVRFVAAADPLAPPTLVSPRREK
                     REYALDAAHGRLWILTNDDHVNFRLAQADLARPGEWTTVIPGSDRVYLRGVSAYRDHL
                     AVTERVDGLDRLRLRGYDGSEDLIPFGEAAYTAGFGGNPEFAPAAYRLHYSSMVTPTT
                     VFDYHPAERRLETLKVQEIPSGYDPSLYETERLMLPARDGKDVPVSVVYRRGFPKDGS
                     GRLFLYAYGAYGYAVPPSFSTSRISLLDRGFAFAIAHVRGGDDLGHGWFLDGKLDKRV
                     NTFNDFVDAARGLIDAGFARPGRIAIQGGSAGGELMGVVANTDPELWGAVVADVPFVD
                     VLNTMQDESLPLTPGEWPEWGNPIEDKAAFELIRSYSPYDNVSAQAYPPMLITGGLND
                     PRVTYWEPAKWAAKLRSAKTDDNLLLLKINMGAGHGGKSGRWDHLREIAEAYAFVLTQ
                     MGGA"
     gene            7941..>8759
                     /locus_tag="VF547_09615"
     CDS             7941..>8759
                     /locus_tag="VF547_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX8263116.1"
                     /translation="MSTYEARLAALREQLKSDRLDGFVVPLTDEHMSEYVGSYAQRLA
                     WLTGFQGSAGAAAVLPEEAAIFVDGRYTLQVRDQVDGKHWSYQSVPETSIADWLKVHA
                     PDGGRIGYDPWLHTKDWVARAKEALAEKGAELVPVQRNPIDAVWADKPEPSKARLVVH
                     PDDFTGKSSAAKRQEMADWLHEQKADAAVLSALDSIAWTFNVRGQDVEHTPVALSFAL
                     VNDDGTADLFVASEKIGDDVRQHLGNGVRVHERDAFEPFLRSLGGRTVIADPERA"
BASE COUNT         1322 a         3044 c         3029 g         1364 t
ORIGIN      
        1 tggcggagac ggagggattc gaaccctcgg taggagtaat ccccctacgg cggtttagca
       61 aaccgctggt ttcagccact cacccacgtc tccgggcgcc gaagcgtcga gcctatatag
      121 ggccgatccg cggggttcaa ccgatcctga aaaagcgaat tgcgaggacg cccggccttg
      181 cggtccgagc cggcggcgat agtctcgacg gcggtgagtc ggcctggggg ggaaggatgt
      241 caccgaacga ccagctttcg atcctgagcg gcgccggcct gctgatcacg ttcagcggcg
      301 tcctgatctc gcagctgacc cgggtttccg tcgacggcga gggcaaccgc aaggtgctga
      361 ctccgaccgg acggctcgcg atcgcgatct ccgtcatcgg gctgttcgga gccgtcgcct
      421 ccgaggctct tcgggcctcg atccgctggc agagcgagaa ggccgcggcg gtcaaggcgg
      481 cggtcgagga acagcgccgc ctcgacgacg aagcctggaa gacccgaagc gagcagctgg
      541 ccacggcgat cttcggcaac accgagaccc agctcgaccg gaccgagcgg aacctgcgcg
      601 agatgctcgc cgggttcagc gccaaccagg agtcgatctt ccgcactcag ctcgccgtcg
      661 tcgaagcgcg gcaacgcgtc ctggccgaca atctgcttcg ggaagcacgc ctttacggcc
      721 gcctcagcgc cgcctcgtcc ccgctcttcc gattgacgct cgagctcgag atcggccggg
      781 cgccgcccga gctcctcgcc gcggtcaggc acggacttcg gcacagccgg gatttcttca
      841 aggattccag ctatatagac ctgaacgagc atcataatat cgacagccgc gacgacatgg
      901 ccatggcgcg ccgggagctc tatcgccagg cggtccagcc ggttctcagt tggatcgcga
      961 acgactccat ccaatcggcg cagagcgtca tgatcgtctc cctggacgac cagttttcgg
     1021 cggtttcggc ggtcggctgg gcgcacgggc cgccgctcgg cgacgaggac aattcggccc
     1081 tcttcaacta tgagaaggac ttctcctggg tgccgagcgg cctgctcggc ggaccgctcg
     1141 aatggaacgc ggcgctgggg gcgggcgacc agtcccacta tcccgcggtc gacgtgcgcg
     1201 tggccggctc gtccatcttc ctgagggccg acgtcagccc ggccgcgctc gaccgcgcga
     1261 tcacgcgcta cgccaaggcc agtgcgacga ccgcgaccct gcccgatcag cttttcgtcc
     1321 tgttctggtc cccggtccct cccggcgaga ccaagccctt cgatccgccg aagctgccgc
     1381 tggattcgaa gggcgtccat gacgtcgccc ggtacgtcgc ctcgggtggc cgcacccggt
     1441 cgagcagccc gcccggtacc tggggacggt cgctccggat ccggctcatc ccgaacggcg
     1501 tggaggcgat cgccaagacc taccggctcc ggccgaccgg cgacggcgag ctgttcgacg
     1561 gcattcgcga cgacgaatat ggcggctatc tgcgcgcgtg gcgcggatcg gcccgctaac
     1621 cgccgccgga tcggccgagg tcgattcgcg gccgccgggt tgagttttgg tcgtcccctg
     1681 ttagtcgact cctcatgctc agcctccagt tcattcgcga ccatcccgac accgtgcgcc
     1741 aggccgccga ggcgaagaac gcggcggtgg acctcgaccg gctgctcgcg ctcgacgggg
     1801 aagtgcggtc gctgaagacc cggatcgacg agctcaggcg ccagcgcaac gagatcagcg
     1861 cgagcttcaa gaccgccgac cccgccgagc gcccggcgct cggggcgaag gccaaggcgt
     1921 tcggagcgga agccgcggac tcggaggcgg cgctggcgga caggtcggcc gagctcgagg
     1981 cgctgatgct gcgggtgccc aacatcccct gggagggcgc tccggtcggg ccggacgaga
     2041 gcttcaacaa ggtcgttcgg accgagggcg agccgcctcg cttcgacttc gagccgcgcg
     2101 accatgtcgc gctggccgag atgaacgact gggcggacct gtcgcggatc acccaggtgg
     2161 cggggtcgcg catgtacgcg ctcaaggggc ggctggcgct gctcgagcag gccctgatgt
     2221 tctgggccca gcagatcatc gccgccgacg gcttcactct gatgaccgtc ccggccctgg
     2281 ccaagccgca ggccttcgtc gccaccggac attttccggg gcacgaggag gaggcctatg
     2341 aagtcgcgaa ggacggcctc tacctcgccg gcaccgccga gatcgccctg accggcctcc
     2401 actcgggcga gatactcgag gccgcgcagc tccccatcct ctacgccggc tattcgccct
     2461 gcttccgccg cgaggcgggc agcgccggcc gcgacgtgcg cgggctgctc cgcgtccatc
     2521 aattctacaa gctcgagcaa tatgtgatct gcgccgacga cgagtcggaa tcgcagcgct
     2581 ggcacgagcg gctgctcgcc aatgccgagc ggctcctcca ggggctggag ataccctatc
     2641 aggtggtcga gacctcgacc ggcgacatgg gcctgggcaa gttccggatg aacgacatcg
     2701 agagctgggt gccgtccttg ggcaagtatc gcgagacgca cagctgctcg acgcttcacg
     2761 actggcaggc ccgccgcgcg aacatccggt ggcgggacgc agagcgcaag gtgcgcttcg
     2821 tccacacgct caacaacacg gcgctcgcca gcccgagaat cctcgtgccg ctgctcgaga
     2881 accaccagca ggccgacggg cgggtgcgcc tcccggcggc gcttcggggc ctgatgggtg
     2941 gcgactatct ctgacacgga gcggccgccg ctgcggctgc tgatgcgcga gggcgcgatg
     3001 atcgcctcgc gcgtctggcg cttcttcggg cgggtcggtg aacgcgggcc ggccgacggg
     3061 ccgccgctga tggtgatccc cggcttcctg gccagcgatc gcagcaccct cggcctgcag
     3121 cgggcgctcg cgcgggcggg cttccgggcg accggctggg gcctcggcat caatcgcgga
     3181 gtccggcccg acacgctcga caagatcgcc gccgaggtcg agcggcacgg ccgcggccgg
     3241 ccggtcgtcc tcgtcggctg gagcctcggc ggagtcttcg cccgcgaggt cgccaagcgg
     3301 cggccggacc tggtcgagaa ggtggtgacg ctgggcgcgc ccttttcggg cgacccgcgc
     3361 gccaacaatg cctggcggct ctacgagctg atcgccggcc acagggtgga tgcgccgccg
     3421 atcgacgcgc gtcccggcga gaagccgccc gtgccgacgc tcgccttgtg gtcgaggcgc
     3481 gacggggtcg tggccccggc ctgcgcccgc ggccgggacg acgagagcga ccatcgggtc
     3541 gagctcgact gcagccatat gggcttcggc gtcagcagcc gcgccttccc caagatcgtc
     3601 agcgcgatca tcgccttccc gggcacctga ccccttactt cccgaccagc ctctgataag
     3661 gtgcgcggcg ggggaatttc atgcgctata tccttgctcc ggagtggagc cacctggcgg
     3721 ccgcgaccgc ggcggcgttc gtcctgggct atctgctgtg ctggatcatg gacaggctgt
     3781 tcctcaagga gctggtcgac gaccgcatcg cgggcatcgc cctgagctgc tgcctcgcct
     3841 tcgtcgtgat catggcctgg gggaccatcg tcctcaccgg cctcgcctgg tcggatccga
     3901 tcatctacgg ccggccgatc gtcattccgc ctttccccta tgccgtcagc ctcatcctcg
     3961 ggctcgcggg ggtcgggatc ctgcgcacga tcctccacgg ccgcgaatat gcggaaggcg
     4021 aggaggagct cgtcttcgac gaggacctgt acgaccaggc gcaatatgac gaggaggtcc
     4081 tcgcgttcga cgagaggcac gcgcacaagg attatttccg ccgccactgg gccggccacc
     4141 tgctgctgcc ggtaaccttc tgggtcaatt tcgtgctggt cgcggtcctg atcgccgccg
     4201 cggtcgaata tgtgggaaac aggctccgcg acaatggcgg caccctgcgc ggcctggcca
     4261 tcatcgcgct cgcctacggc gtcttctcga tcatcttctg ggcgtggagc tcggtcggaa
     4321 tctggcgctc ggcctattgg caccgccggc ggggcgggac tccgggttgg gggctcgccg
     4381 cccgcatcct ggtggtggtg ggcggattgg tcattctcgt gcgatccggc gacatcgcgc
     4441 ttcggacccg tgagctgggg actctcgccg ccggcgggga cgtgatcggc cccgtcgcgg
     4501 agatgaaagt gtccggcgac ggccgcgacc tttccgtccg cggcagcctc gccgccgggt
     4561 cagcggagcg cttcgaggcc ctgctggcgg ccaaccccga cgtgcgcaga gtggtgctgc
     4621 actcgcccca aggccgctat ttcgaggcgg agcggatggc cgcgctggtc cgcagccgcg
     4681 gcctcgacac tcgggtggac gacgtctgcc tgtcgccctg caccgacctg ctggtcgccg
     4741 ggcgcgagcg gaccgcgccc gagcaggccc ggatcggctt tcaccagccc agcttcccgg
     4801 ccgacgacgg ctacggcatg cgcaaggccg tcgagcgcct gcgcgccgac tatctcgccg
     4861 cgggcgtgaa gcgcgagttc gtcgaccggg tcctcgcgac gccgccgccg accctgtggt
     4921 tccccgaccc ggtcgagctg gtcgaggcga acctgctcac cggctcggac gtcttcgtga
     4981 cgaagaacgg cgccaggcgg cgggagaatg cggtcgagat gcgcctgcgc cgcaacatcg
     5041 ccgcgacggc cgagcggatc aacgcgaccc tgccgaggcg gctcagcgcc gacacgacgc
     5101 tcctgcgcgt gtccgcctcg ggcacgacgc tcacccatca ttatcggatc gacgccgaga
     5161 agatcgacgc cgccggcacc cggatcggcc tcgggcagca gcttcgccgg cagatgtgcg
     5221 cggacgtcga catggctctg gcgatccgcg aaggcgcccg gttcgtgcat tcctacagcg
     5281 accggcgggg ccgtcacgcg gtcgacatcg ccgtcaccca atgtccctga ggaagggcgc
     5341 cgccacctcc tccggcaccc gcagcggccg gccggtggcc ttgtcgagga tcgcccaggt
     5401 cgtgcgggcc cggacccgcg gcttgccgtc gccgccgacg aactcgacat ggcggtcgaa
     5461 gcgggcgccg cgcggcgcct cggcgaccca ggtccgccct gtcaccgtct cgccgggaag
     5521 gacggcgcgc agatagtcga tctcgtggcg gacgacgacc cagacatagg cgtcgacatg
     5581 gccggctcgg ccgccgagct ccagtgcgcc gtcgccatgt cctggatcca gcgcacccac
     5641 accgcattgt tgacatggcc gagctcgtcg acgtcctcgg ccgaggcggt gatgctccgc
     5701 tcgaagaacg ccgtcacgcg ccgcccatct gggtcaggac gaaggcatag gcctcggcga
     5761 tctcgcggag atggtcccag cggccagact tgccgccgtg gccggcgccc atgttgatct
     5821 tgaggaggag gagattgtcg tcggtcttgg ccgagcgcag cttcgccgcc catttggcgg
     5881 gctcccaata ggtgacccgc ggatcgttga ggccgccggt gatcagcatc ggcggatagg
     5941 cctgggcgct gacgttgtcg taggggctgt agctccggat cagctcgaac gccgccttgt
     6001 cctcgatcgg attgccccat tcgggccatt cgccgggggt cagcggcagg ctctcgtcct
     6061 gcatcgtgtt gaggacgtcg acgaacggca cgtcggcgac gaccgcaccc cacagctccg
     6121 gatcggtgtt ggcgaccacg cccatcagct cgccgccggc cgagccgccc tggatggcga
     6181 tcctgcccgg ccgcgcgaag ccggcgtcga tcaggccgcg ggcggcgtcg acgaagtcgt
     6241 tgaacgtgtt gacgcgcttg tcgagcttgc cgtcgaggaa ccagccgtgg ccgaggtcgt
     6301 cgccgccgcg gacatgggcg atggcgaagg cgaagccgcg gtcgagcagg ctgatccggc
     6361 tggtcgagaa gctcggcggc accgcatagc cgtaggcgcc gtaggcgtag aggaagagcc
     6421 gtccgctgcc gtccttcggg aagcccctgc ggtagacgac cgagaccggg acgtccttgc
     6481 cgtcgcgcgc aggcagcatc agccgctcgg tctcgtagag cgacggatcg tagcccgacg
     6541 ggatctcctg gacctttagc gtctccagcc gccgctcggc cgggtggtag tcgaagaccg
     6601 tcgtcggcgt gaccatcgac gaataatgga gccggtaggc ggccggggcg aattccggat
     6661 tgccgccgaa gccggcggta taggcggcct cgccgaacgg gatgaggtcc tcgctgccgt
     6721 cgtagccgcg cagccgcagc cggtcgaggc cgtcgacccg ctcggtgacg gcgagatggt
     6781 cgcgataggc cgagaccccg cgcagataga cccggtccga gcccgggatg acggtggtcc
     6841 attcgccggg acgggccaga tccgcctgcg cgaggcggaa gttgacatgg tcgtcgttgg
     6901 tcaggatcca cagccggccg tgggccgcgt cgagagcata ttcgcgcttc tcgcggcgcg
     6961 gcgagacgag ggtgggcggc gccagcggat cggccgcggc gacgaatcgg acctcgctgg
     7021 tggcgttgtc gccggtggcg atgaagatca ggctccggtc ctgcgagcgc gacactccga
     7081 ccgagaagcc gagcgcctcg gtctcctcgt acaaggtgac gtcgtccgag gcgggcgatc
     7141 cgagccggtg gtagcgggcg cggtagcttc gccaatgctc gttgacctcg gtgaagacga
     7201 tcccctcgcc gtcgcccgac cagaccggat ggccgatggc gacgtcggtg accgtctcca
     7261 gatcctcgcc ggtggcgagg tcgcggatgc gcaggtcgaa gcgttcggag ccgctgtcgt
     7321 cgacgaggat ggcggcgagc cggccgtcgg ggctgacctc gagcgctccg agccggaaat
     7381 attccttgcc ctccgcctcc gccggctcgt cgaagacgac ctgctcggcg ccgcccgcgg
     7441 cccggcgccg gtaccaggtg cggtactgcg cccccggctc gaacgcccac cagtagagcc
     7501 agtcgccgtc gcgcaccgga accgacgatt cgtcctcctt gagccggccc ttcatctcct
     7561 cgaacagggt ctcgaccagc gccgcgtgcg gcttcatcgc cgcctcgaaa taggcgttct
     7621 cggccttgag ataggccagg acgtcctcgt cctcgacgga cgggtatccc ggatccttca
     7681 gccaggcata ggggtcctcg accctgtagc cgtggcgttc gtaggaaaag gggcgcggct
     7741 cggcgcgcgg cggggctgga agcggatcgg tcatgaagct ttctgggttt cgacgcgtag
     7801 ttgcacagcg caggagaatg gctaagtcag gtccatcccg ttcgggctga gcctgtcgaa
     7861 gccctgccct tcttcagggt ttgggtaaag agcggcttcg acgcgctcgg cccgaacgga
     7921 tgcaatcacc ggaggcctct atgtcgacct acgaagcccg cctcgccgcc ctgcgcgagc
     7981 agctcaaatc ggaccggctc gacggcttcg tcgtgcctct gaccgacgag catatgagcg
     8041 aatatgtcgg cagctacgcc cagcgcctcg cctggctgac cgggttccag ggctcggccg
     8101 gggccgcggc cgtgctcccc gaggaggccg cgatcttcgt cgacggccgc tacacgctgc
     8161 aggtgcgcga ccaggtcgac ggcaagcatt ggagctacca gtcggtgccc gaaaccagca
     8221 tcgccgactg gctcaaggtc cacgcccccg acggcggccg gatcggctac gatccctggc
     8281 tccacaccaa ggactgggtg gcccgggcca aggaggcgct ggccgagaag ggcgccgagc
     8341 tggtccccgt ccagcgcaat ccgatcgacg ccgtctgggc cgacaagccg gagccgtcca
     8401 aggcgcgcct cgtcgtccat cccgacgatt tcaccggcaa gtcctccgcc gccaagcgcc
     8461 aggagatggc cgactggctg cacgagcaga aggccgatgc ggcggttctc tccgcgctcg
     8521 attcgatcgc ctggaccttc aacgtgcgcg gacaggacgt cgagcatacg ccggtcgcct
     8581 tgtccttcgc cctggtcaac gacgacggca ccgccgacct gttcgtcgcc tcggagaaga
     8641 tcggcgacga cgtccgccag catctcggca acggggttcg ggttcacgag cgcgatgcgt
     8701 tcgagccgtt cctccgctcg ctcggcggcc gaaccgtcat cgccgacccg gagcgggcg
//
LOCUS       DASVQZ010000338         7698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_747261, whole genome shotgun sequence.
ACCESSION   DASVQZ010000338 DASVQZ010000000
VERSION     DASVQZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747261"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VF547_09620"
     CDS             <1..1324
                     /locus_tag="VF547_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX8263117.1"
                     /translation="IGKEAGEATVIATAADGSVVYGAHVRVSQNISSVDEMLRAAMPD
                     SNISVTHVGQIAVINGTVASPADVAQAEQLVRMMLNPGIDTNTPGAALRIVPVNRLKT
                     ATPLQVMLHVKIAEVSRSLARQIGVNLATSDPTGGAFSFALSRGRNLGDINAGVLAPA
                     GQTTIAGLARNVFGLNVAGALDLNENNGLVTTLAEPTLVALSGETASFLAGGEFPIPT
                     SNGINGTAIEFKPYGVSLAFTPTVLEGGRISMRVRPEVSELSSAGAIKVNGFEVPSLT
                     TRRTETTVELGSGQSFMIGGLLSNGGNNTIERTPFLGNLPIIGALFRSNTFRRNESEL
                     VIVVTPYLVNPVTASQIALPTDGYRGANTAERVFLGTAEYGRTGAQRPMPTMVPAQSA
                     APAMGGIAAAPASAPAQLPAPQRRAEKPAATRPEQAPAAASAAPGFSF"
     gene            1347..1988
                     /locus_tag="VF547_09625"
     CDS             1347..1988
                     /locus_tag="VF547_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HEX8263118.1"
                     /translation="MARSKSLAALVAIGAAVSLASAADARPERHGPERGLDSLNQPVV
                     VRTDYVLDLSAPDGRLSAGERARLHKWFTSLDLGYGDHIFVDEGEGYGYGRRDVAAVA
                     AEYGILLSDGAPVTAGSVPPGVVRTIVSRSTASVPNCPNWASRGPSSTSSNYGCAFNS
                     NLAAMIADPNDLVLGQSGSVTGDASTSSKAIRVYRQTAPSGTKGLSEISTRGN"
     gene            1989..3272
                     /locus_tag="VF547_09630"
     CDS             1989..3272
                     /locus_tag="VF547_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HEX8263119.1"
                     /translation="MNAPFHASRAAALRDPFSAFVCDDATADMLRPIAVEHGWAPEKV
                     NKGGLRNAVQTLSVSASPAILFVDLSESGDPLNDINALAEVCEPGTVVIAAGQVNDVR
                     LYRDLVASGLQDYLLKPFTPDQLRDTFAHAQLTISGPRVNEAASEKPHVMAAVIGVRG
                     GVGASTLATSLAWMMGDKTGRSTALLDLDIHFGTGALSLDLEPGRGLTDAIENPSRID
                     GLFIERAMVRANEKLCVLSAEAPLNQPLLNDGTAFFQLQEELRNAFESTVLDLPRSML
                     VQHSHLVQDVHVVVVAVEFTLAATRDAIRVLSWLKANAPQARVLVVANRVPSGAQEIS
                     RKDFEQSIERGVDIVIPFEPKAAAQAAKLGQPFAKVATSSKVQGPLTQLVNLTVSTVE
                     GSEGGGDKAPDTPKSLLDKLNLKGLVAKKPKPATA"
     gene            3362..4339
                     /locus_tag="VF547_09635"
     CDS             3362..4339
                     /locus_tag="VF547_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8263120.1"
                     /translation="MLSFPILMLGGGVLAVLAMMAFAMGGPNVAKATNRRLEVVRERH
                     SKSNEVAAQAQLKRILQTRNQTKMDGLAQRLIPKPALLRQRLQQTGRTWTLAQYGLAC
                     LGLIAVVALLLIVKGLPILLAVLIGLFAGIGLPHFVVGKLIKRRVAKFTARFPDAIDL
                     MVRGLRSGLPISETLGVVADEIPGPIGDEFRTVSDKMKIGRSMEAALQETADRLGTPE
                     FQFFVITLAIQRETGGNLAETLANLSEVLRKRAQMKLKIKAMSSESKASAWIVGALPF
                     IVFILIWFINGKYMQTFFTDERLMIAGGGGLIWMGIGVFIMAKMVDFEI"
     gene            4382..5350
                     /locus_tag="VF547_09640"
     CDS             4382..5350
                     /locus_tag="VF547_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8263121.1"
                     /translation="MGVDVVMVATFMAAVATFAVLVAIYAATTVKDPMAKRVKALNER
                     REQLKAGIVASTSKRRRNITNKNEMADKVRGVLGSLKMLQDEQVEKAQKKLMQAGVRS
                     KDLAFVVIFARLVLPLVIGTTVIVGVYLLDWFPQWGAFKRYALVAGSLLLSYKAPDLW
                     LKNKISKRSNNVRKGLPDALDLLVICAEAGLTVDAAFSRVARELGKAYPELGDEFALT
                     SIELGFLTDRRSAFENLANRIDLDAVRGVVTTMIQTEKYGTPLASALRVLAAEFRNER
                     MMRAEEKAARLPAIMTVPLILFILPVLFIVILGPASCSISDNLFKT"
     gene            5551..5799
                     /locus_tag="VF547_09645"
     CDS             5551..5799
                     /locus_tag="VF547_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263122.1"
                     /translation="MTLFTPDQWLIVGLIFLLGIVLGMALMAGGKWKRRYKEERARCA
                     ELEAENRRLQKDAGEMDSLRQAAARDEARRRTDGPGPL"
     gene            5882..6334
                     /locus_tag="VF547_09650"
     CDS             5882..6334
                     /locus_tag="VF547_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263123.1"
                     /translation="MGRLRATIAGLAALAPAAAAADIPLRQRLFCAELDRVVQVAQMD
                     GDFTQLERSRAAPPSFGFRHGCRAHGASERLPAAWWCHQSLAPAELALDSLGALTAGC
                     LPNAVRSRGRYGEAVFTLPRARIRIEERGGPRAHVGRIVTFRVEAVRP"
     gene            complement(6422..7000)
                     /locus_tag="VF547_09655"
     CDS             complement(6422..7000)
                     /locus_tag="VF547_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263124.1"
                     /translation="MNRRVFISTMVSAAFLAVVPARAAEPGDWEVFGRLADAVASLLT
                     VGDPIAETVNRLKFRRFLRAVENSVAEIADSKFTVLEMLQLPTCSSDPGDRIMAARAA
                     ALRIIPLVDQLAAHVRTFATAVRPSGTREDIVRIAEDFRSMQHRKMWVYRIDTYCAET
                     PEGRFAFRKEVAGSYDLVRRSQRSIGLLLERL"
     gene            complement(6997..7185)
                     /locus_tag="VF547_09660"
     CDS             complement(6997..7185)
                     /locus_tag="VF547_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263125.1"
                     /translation="MPVAKRHLAVFLLFLVAAATVSFLMPLDVDSKDVFSYFMLGFMA
                     YLALSYAATLLWPRKQRP"
     gene            complement(7278..>7698)
                     /locus_tag="VF547_09665"
     CDS             complement(7278..>7698)
                     /locus_tag="VF547_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3833 family protein"
                     /protein_id="HEX8263126.1"
                     /translation="TAPEQFFVGRTEGSGTVQMTLAGSHKVRDVSRGRLDKGGALILE
                     QMVYEEGKPARRRSWRLARAGGNRIAGTISDAVGPVTGEMKGNVLHLKYRAREANAQV
                     EQWITLHPGGRTASNRMIFRKWGLKAATLESTIRKLD"
BASE COUNT         1269 a         2769 c         2469 g         1191 t
ORIGIN      
        1 gatcggcaag gaagccggcg aggccacggt catcgccacg gcggcggacg gttcggtcgt
       61 ctacggagct catgtccggg tcagccagaa catcagctcg gtcgacgaga tgctgcgcgc
      121 cgcgatgccg gattccaaca tcagcgtcac ccatgtcgga cagatcgcgg tgatcaacgg
      181 gacggtcgcc tcgccggccg acgtggccca ggccgagcag ctggtccgga tgatgctcaa
      241 tcccggcatc gacaccaaca cgccgggcgc cgcgctgagg atcgttcccg tcaaccggct
      301 gaagaccgcc acgccgctcc aggtgatgct gcacgtcaag atcgccgaag tgagccggtc
      361 gcttgcccgc cagatcggag tcaatctcgc gacctcggat ccgacgggcg gcgccttcag
      421 cttcgcgctc agccgcggcc gcaacctcgg cgacatcaac gccggagtcc tcgcccccgc
      481 cgggcagacg acgatcgccg gtctagcgcg caacgtgttc gggctcaacg tcgccggcgc
      541 gctcgacctc aacgagaata acggcctcgt caccaccctc gccgagccga ccctggtcgc
      601 gctctcgggc gagaccgcga gcttcctcgc cggcggcgag ttcccgatcc cgacctcgaa
      661 cggcatcaac ggcacggcga tcgagttcaa gccctacggc gtcagcctcg ccttcacccc
      721 gaccgtcctc gaagggggcc gcatctcgat gcgggtccgt ccggaagtgt cggagctgtc
      781 gtccgccgga gcgatcaagg tcaacggctt cgaggtgccc tcgctcacca cccgccgcac
      841 cgagacgacg gtggagctcg gctccggcca gagcttcatg atcggcggcc tgctcagcaa
      901 cggcggcaac aacacgatcg agcgcacgcc cttcctcggc aatttgccga tcatcggcgc
      961 cctgttccgt tccaacacct tccgccgcaa cgagtcggag ctggtgatcg tcgtcacgcc
     1021 ctatctggtc aacccggtca ccgcgagcca gatcgcgctt ccgaccgacg gctatcgcgg
     1081 cgccaacacc gccgagcggg tgttcctcgg caccgccgaa tatggccgca ccggcgccca
     1141 gcggccgatg ccgaccatgg tcccggcgca gagcgcggcg ccggcgatgg gcggcatcgc
     1201 agcggctccg gcctcggcgc ccgcgcagct gcccgcgccg cagcgccgcg ccgagaaacc
     1261 ggccgcgacc cgccccgagc aggcccccgc cgccgcttcc gccgcccccg gcttcagctt
     1321 ctgaccgatc gataaaggac gtgatcatgg cccgcagcaa atcactcgcc gccctcgtcg
     1381 cgatcggcgc cgccgtcagc ctcgcctccg ccgccgacgc gcggccggag cggcacggcc
     1441 cggaacgagg gctcgacagc ctcaaccagc cggtggtcgt tcgaaccgat tatgtgctcg
     1501 acctctccgc ccccgacggc cggctgtcgg cgggcgaacg cgcccgcctg cataaatggt
     1561 tcacctcgct cgacctcgga tatggcgacc acatcttcgt cgacgagggc gagggctatg
     1621 gctatggccg ccgcgacgtc gccgccgtgg ccgccgaata tgggatcctg ctcagcgacg
     1681 gcgctccggt gacggccgga tcggttcccc ccggagtcgt ccggacgatc gtcagccgca
     1741 gcaccgcctc ggtgcccaac tgcccgaact gggcctcccg cgggccgagc tcgacctcgt
     1801 ccaactacgg ctgcgccttc aactccaatc tcgccgcgat gatcgcggac ccgaacgatc
     1861 tggtcctggg gcagagcggc agcgtcaccg gcgatgccag cacttcctcg aaggcgatcc
     1921 gggtctatcg gcagaccgct ccgagcggaa ccaagggtct ctccgagatc agcaccaggg
     1981 gtaattagat gaacgcgcca ttccacgctt cccgcgctgc cgcgcttcgc gacccgttca
     2041 gcgccttcgt ctgcgacgac gccaccgccg acatgcttcg gccgatcgcc gtcgagcacg
     2101 gctgggcgcc cgagaaggtc aacaagggcg gccttcgcaa cgccgtgcag accctgtcgg
     2161 tctcggcgag ccccgcgatc ctgttcgtcg acctgtcgga atcgggcgat ccgctgaacg
     2221 acatcaacgc gctggccgaa gtctgcgagc ccggcacggt ggtgatcgcc gccggccagg
     2281 tcaacgacgt gcgcctctac cgcgacctgg tcgcgtccgg gcttcaggac tatctgctca
     2341 agcccttcac gcccgaccag ctgcgcgaca ccttcgccca cgcccagctg accatctccg
     2401 gtccaagggt caacgaggcc gccagcgaga agccgcacgt catggcggcc gtgatcggcg
     2461 tgcgcggcgg agtcggcgcc tccaccctcg ccacctcgct ggcctggatg atgggcgaca
     2521 agaccggccg ctccacggcc ctgctcgacc tcgacatcca cttcggcacc ggcgcgcttt
     2581 cgctcgatct cgagccgggc cgaggcctca ccgacgcgat cgagaatccg agccggatcg
     2641 atgggctgtt catcgagcgc gcgatggttc gggcgaacga gaagctctgc gtcctctcgg
     2701 ccgaggcgcc gctcaaccag ccgctgctca acgacggcac cgccttcttc cagctgcagg
     2761 aagagctccg caacgccttc gagagcacgg tgctcgacct gccgcgctcg atgctggtcc
     2821 agcattccca cctcgtccag gacgtccacg tcgtcgtcgt cgcggtcgag ttcaccctcg
     2881 ccgcgacccg cgacgcgatc cgggtcctga gctggctcaa ggccaatgcc ccgcaggcgc
     2941 gggtgctggt cgtcgccaac cgggttccgt cgggcgccca ggagatcagc cgcaaggatt
     3001 tcgagcagtc gatcgagcgc ggcgtcgaca tcgtcatccc gttcgagccg aaggcggccg
     3061 cgcaagcggc caagctcggc cagcccttcg ccaaggtcgc gacctcgtcg aaggtgcagg
     3121 gcccgctgac ccagctcgtc aacctcaccg tctcgacggt cgagggctcg gagggcggcg
     3181 gcgacaaggc gccggacact cccaagtcgc tgctcgacaa gctcaacctc aaggggctgg
     3241 tcgcgaagaa gccgaagccg gcgacggcgt gatgggagtc ggtcctcgcg agaccggttc
     3301 gcttcgcggc gggacggcga gggcgccggc gcgacgtcgg tctgttggat aggagccaag
     3361 ggtgctgagc tttccgatcc tgatgctggg cggcggcgtg ctggcggtgc tcgcgatgat
     3421 ggcgttcgcc atgggcgggc ccaatgtcgc gaaggcgacc aaccggcgcc tcgaggtcgt
     3481 gcgcgagcgc cacagcaagt ccaacgaggt cgccgcccag gctcagctca agcggatcct
     3541 ccagacccgc aaccagacca agatggacgg gctcgcccag cggctgatcc cgaagccggc
     3601 cctgctccgc cagcggctgc agcagaccgg gcggacctgg acgctcgccc aatacggcct
     3661 ggcctgcctc gggctcatcg ccgtcgtcgc cctgctgctg atcgtgaagg gcctgccgat
     3721 cctgctcgcg gtcctgatcg gcctgttcgc cggaatcggc ctgccccatt tcgtcgtcgg
     3781 caagctgatc aagcgccggg tcgccaagtt caccgcccgc ttccccgacg cgatcgacct
     3841 gatggtccgc ggcctccgct ccggccttcc gatctcggag accctgggcg tcgtcgccga
     3901 cgagatcccc ggcccgatcg gcgacgagtt ccggaccgtg tccgacaaga tgaagatcgg
     3961 ccgctcgatg gaggcggcgc tccaggagac ggccgaccgc ctcgggaccc ccgaattcca
     4021 gttcttcgtc atcaccctgg cgatccagcg cgagaccggc ggcaacctcg ccgagacgct
     4081 cgccaacctc tccgaggtgc tgaggaaacg cgcccagatg aagctcaaga tcaaggcgat
     4141 gtcgtcggaa tccaaggcct cggcctggat cgtcggcgcc ctgcccttca tcgtcttcat
     4201 cctgatctgg ttcatcaacg gcaagtacat gcagaccttc ttcaccgacg agcggctgat
     4261 gatcgcgggc ggcggcgggc tgatctggat gggcatcggc gtgttcatca tggccaagat
     4321 ggtcgacttc gagatctagg ggcggcttga gatgacagta cccgcaaccg gaccgaccat
     4381 catgggggtc gacgtggtga tggtggcgac cttcatggcc gccgtcgcca ccttcgccgt
     4441 gctcgtcgcc atctacgccg cgaccaccgt caaggacccg atggccaagc gggtgaaggc
     4501 gctcaacgag cggcgcgagc agctcaaggc cggaatcgtc gcctcgacgt cgaagcggcg
     4561 gcgcaacatc accaacaaga acgagatggc ggacaaggtc cgcggcgtcc tcggctcgct
     4621 caagatgctc caggacgagc aggtcgagaa ggcccagaag aagctgatgc aggcgggcgt
     4681 gcggtccaag gacctcgcct tcgtcgtcat cttcgcccgg ctggtgctgc cgctcgtcat
     4741 cggcacgacc gtgatcgtcg gcgtgtacct gctcgactgg ttcccccaat ggggcgcgtt
     4801 caagcgctac gccctcgtcg ccggctcgct gcttctgagc tacaaggcgc ccgacctgtg
     4861 gctcaagaac aagatctcca agcgctcgaa caacgtccgc aagggccttc ccgacgccct
     4921 cgacctgctg gtcatctgcg ccgaggccgg cctgaccgtc gacgcggcct tcagccgggt
     4981 cgcccgcgag ctcggcaagg cctatcccga gctgggcgac gagttcgcct tgacctcgat
     5041 cgagctgggc ttcctcaccg accgccgctc cgccttcgag aatctcgcca accggatcga
     5101 cctcgacgcc gtccgcggag tcgtcacgac catgatccag accgagaaat acggaacgcc
     5161 gctggcctcg gcccttcgag tcctcgccgc cgagttccgc aacgagcgga tgatgcgcgc
     5221 cgaggagaag gcggcgcggc tgccggcgat catgacggtg ccgctgatcc tgttcatcct
     5281 cccggtgctg ttcatcgtca tcctcggccc ggcgagctgc tcgatctccg acaacctgtt
     5341 caagacgtag tccgccctga gccagcgacg cgggcgccta aggcgctcgc aagctggccc
     5401 aggagtcgga caagtccggc cggcgggccg gctggcgcgt ccagccggcg cgttcgacgt
     5461 cacggccgcc cgtgacgtcg ccccccggcg gaaccaatcg tcgcgccccg ccattgatac
     5521 gccgccgcaa caagcgacgg agacaggccc gtgaccctct tcacccccga ccaatggctc
     5581 atcgtcggcc tcatcttcct gctcggaatc gtgctcggaa tggcgctgat ggccggcggc
     5641 aagtggaagc gccgctacaa ggaggagcgc gcccgctgcg ccgagctcga ggcggagaac
     5701 cggcgcctcc agaaggacgc cggcgagatg gactcgctgc gccaggccgc cgcccgcgac
     5761 gaggcccggc ggcgcaccga cggtcccggg ccgctctagg cgagactgcc gccaggcccg
     5821 ttcaggcctg cgtcagccgc ccgacgctct ttttccggct ccacggttgg ggggaggcgt
     5881 tatggggcgg ttgcgggcga cgatcgcggg gcttgcggcg ctggcgcccg ccgcggcggc
     5941 ggcggatatc ccgttgaggc agaggctgtt ctgcgccgag ctcgaccggg tggtccaggt
     6001 ggcgcagatg gacggcgact tcacccagct cgagcgcagc cgggcggcgc cgccctcctt
     6061 cggcttccgc cacggctgcc gcgcccatgg cgcctcggag cggctgcccg ccgcctggtg
     6121 gtgccaccag agcctggcgc ccgccgagct cgccctcgac agcctcggcg ccctcaccgc
     6181 cggctgcctg ccgaacgccg ttcgaagccg gggccgctac ggcgaggccg tcttcaccct
     6241 cccccgcgcg cggatccgga tcgaggagcg gggcgggccg cgcgcccatg tcggccgaat
     6301 cgtcaccttc cgggtggagg cggtgcgccc ctgaacgccg gggtccgccc ccggcttatc
     6361 ggcggagcga gcgagctaat cgagaaggac agacgccttt gcatcgtcca aacgagtagg
     6421 ctcaaagcct ttcgagtaat aatccgatgc tgcgttgcga tcggcgcacc agatcgtaac
     6481 tgccagccac ttcttttcga aaagcgaagc ggccctctgg tgtctcagca caataggtgt
     6541 cgatcctgta cacccacatt ttgcggtgtt gcattgatcg aaagtcctcc gcgatccgca
     6601 cgatatcctc tctggtcccg ctcgggcgga ccgccgtggc gaaggtcctg acgtgcgccg
     6661 ccagctggtc aaccaatgga ataatccgca aagcggccgc ccttgccgcc atgatccgat
     6721 caccaggatc cgatgagcat gtgggcagct gaagcatctc gaggacggtg aattttgagt
     6781 cggcgatctc cgcaaccgaa ttctcgaccg ccctcaagaa gcgtcggaac ttcagtcgat
     6841 tgaccgtttc ggcaatcggg tcgccgaccg tcagcagcga tgccacggcg tccgcgaggc
     6901 gtccaaaaac ttcccaatcg cccggctctg ctgcccgggc gggtacgacg gccaggaagg
     6961 ccgcagaaac catggtcgag ataaaaactc tccggttcac ggccgctgct tgcgaggcca
     7021 cagcaacgtc gctgcatagc tgagcgcgag gtaggccatg aagcccagca tgaaataaga
     7081 gaagacgtct tttgaatcga cgtcgagcgg catcaggaat gagacggtag ccgcggcaac
     7141 tagaaatagc aggaaaaccg caagatggcg tttggcgact ggcatcgatc tcccttcccc
     7201 tttcgggaga atagctcggc cgcgactgga tataaagact tacgtcagca ggaccctcgg
     7261 ccgcaaaatg acagcggtca gtcgagcttg cggatggtcg attcgagcgt ggcggccttg
     7321 aggccccatt tgcggaagat catccggttc gacgcggtcc ggccgcccgg atgcagcgtg
     7381 atccactgct cgacctgggc gttggcctcc ctggcgcgat acttgaggtg gaggacgttg
     7441 cccttcattt ccccggtcac cggcccgacg gcgtcggaga tggtcccggc gatgcggttg
     7501 ccgcccgccc gcgccagccg ccacgagcgg cgccgggcgg gcttgccctc ctcgtagacc
     7561 atctgctcga ggatcagcgc cccacccttg tcgagccgcc cccggctgac gtcccgcacc
     7621 ttgtgcgaac cggccagggt catctgcacg gtgcccgagc cttcggtccg gccgacgaag
     7681 aactgctcgg gggcggtg
//
LOCUS       DASVQZ010000339         8038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_747485, whole genome shotgun sequence.
ACCESSION   DASVQZ010000339 DASVQZ010000000
VERSION     DASVQZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747485"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VF547_09670"
     CDS             <1..1181
                     /locus_tag="VF547_09670"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263127.1"
                     /translation="AATGLQSTAVGDVASALATNSSAFGRNANVAAAATNGTALGGET
                     VSGGVNSTAVGWRAAATGASSTALGDRAVASGNFSTALGDFAAATGLQSTAVGDVASA
                     LATNGSAFGRNANVAAAATNGTALGSESAAGGVNATAVGWRTSATGQSSTALGDRAVA
                     SGNFSTALGDFAAATGLQSTAVGDVASALGANSTAVGRDANATAANSTAIGNAARATH
                     ANSTAIGNGATTTAANQVTVGGAGSTVRFGGYTTAGVLVNDANGNISTNTTLLGQVAT
                     NTANITTLQGNVTNLQTLTATHTTQIADLFDLSDINRRDIRKANEGVAMALAMETPHL
                     PAGTTMGVAGGIGYYQNRLAGTASFAARVGTNASFSAGVGLGFDSGEIGARAGFQTAW
                     "
     gene            1175..1714
                     /locus_tag="VF547_09675"
     CDS             1175..1714
                     /locus_tag="VF547_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263128.1"
                     /translation="MVKLRFRAKVGPGFFTPARLFFAACLAASAAPAAAQAPAPAPIA
                     NQAGVPDPIVVNKLVWSAMAALDQANQTGNYSVLRDLGAPSFQTNNSAATLGGIFQAL
                     RAQQVDLGYTLLVAPSFQFPPAIVQGGLLRVRGTFPLRPAAIGFDLLFQNISGQWRIF
                     GIAVAPIVAQAPQSGSTRR"
     gene            1984..3894
                     /locus_tag="VF547_09680"
     CDS             1984..3894
                     /locus_tag="VF547_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8263129.1"
                     /translation="MTAIAGFWSFDGRFDPSAQCEAMIAAQSIYGLRQSAARLDRLAL
                     AARLYPLLPEDAHDRQPLVGGDGRFALVADVRLDNRAELAGALGLAPAETASLADSDL
                     LLRALERWGEDAVARLLGDFAFAFYDAREATLLLARDPLGQRPLFWHRGAGLAAFASM
                     PAGLHALAAVPRRRDETSLIRYLASLPPLGPSSFHEGIERVEPGHLVTLTPSRTIRRR
                     HWRPQRRDLGLRRFDDYVEAWRSELDAAVARRLRGAGGLVAAHLSGGWDSNAVAATAA
                     RLTAAGGGRVVAFTHVPRPEARAGSPSNRFADEGALAAATARLHPNMDHSILPSSCRS
                     PLADLDRYAALFDRPVFNLCNHVWLSEIRAAASAAGARVLLSGEIGNWTISAAPNALL
                     ADFLREGRLIAWARAARGLVRTGRARWRGVAASSFGAWVPDSAWRRLSGLASGPEASS
                     TDPVHPQLRDAVEAARQADGGGIERRRTAFEDAADALQAMDWGEHRKGILGGWGLDKR
                     DPTADVRLVDFCLSLPLDMLMKDGVRRPLARAALADRVAPAVLDERRRGYQSADWHVG
                     LTRDRATAVRLLGAMADDSAASRLIDVERLQRLVRDWPDGGWESARTIGAYRIGLLKG
                     LTAGHFILAAGR"
     gene            3958..4887
                     /locus_tag="VF547_09685"
     CDS             3958..4887
                     /locus_tag="VF547_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263130.1"
                     /translation="MLAPEGARRASTYRLFGLDLRSEIDLAGLAPTVDSGSPDVEIAF
                     GAVPASDHNGYRPTEDGTLLAVAKVGRYLIREGRQILIDPAPDASERNVRLFLLGSAI
                     GALLHQRGLLPLHANAIDLGGRAVAFSGHSGAGKSTIAAWFHDRGHRILADDVCVIGF
                     DAAGRALAYPGIPRLRLWREALEASGRDADAYSRSFDDTDKYDVPTANEGGLDPIPLA
                     AIYLLRKAEEEAGEAEIERLTGVDAVETLVSNTYRGGYLSMIGRTSQHLAACIRVARA
                     VPVFRAQRLWGFERFDEQAGRLRDHASAQLVHG"
     gene            4942..7074
                     /locus_tag="VF547_09690"
     CDS             4942..7074
                     /locus_tag="VF547_09690"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX8263131.1"
                     /translation="MSGYELISGIEVDRGLKAFVDEEALPGTGIDPAAFWFGFSALLR
                     ELTPENKRLLRKREDLQARIDARNRSLGGRPADPAEEEEFLREIGYLVDPPARFAIAT
                     SDVDPEIARIAGPQLVVPVSNARYALNAANARWGSLYDALYGTDALGDRPASGGYDAE
                     RGARVVAWGRAFLDEIAPLTGGSHADVAAYRIEDSRLVTDRGTLRDPALLAGWRGESP
                     LLRHHGLHVEILVDRDHPIGRGDRAGVADIVLESAVTAIMDCEDSVAAVDAEEKVGVY
                     RNWLGLMKGTLSAGFDKGGRSVDRRLAPDRVFEAPGGGTLTLKGRALMFVRNVGHLMA
                     NPMMRIDGEDVREGLVDAVITGLAALHDLNGGRANSPAGSVYIVKPKMHGPEEAAFAG
                     RLFDRTEDLLGLPRHSLKIGVMDEERRTSVNLAAVIHAVKDRVVFINTGFLDRTGDEI
                     HTAMRLGPVIRKAEMKSSAWLKAYEDNNVDVGLACGFAGRAQIGKGMWAMPDLMADML
                     AQKIAHPQAGATTAWVPSPTAATLHAVHYHRVDVAERQREIAARPAAPLEGLLAVPVA
                     VEPGWSADAVCEELDNNVQGILGYVVRWIDQGVGCSKVPDIHDVGLMEDRATCRISSQ
                     HVANWMMHGVVSEAQVEEALRRMAAVVDRQNANDPAYRPMAADFERSTAFRAARALVL
                     QGADQPSGYTEPLLHAHRLALKAELGRS"
     gene            complement(7048..7962)
                     /locus_tag="VF547_09695"
     CDS             complement(7048..7962)
                     /locus_tag="VF547_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8263132.1"
                     /translation="MPRVIDTDTAEAIQLGDLVEALATGGFDPDDDSSLAAFAPALSR
                     LGNNRSFLGDLVVEELKHSCAGQVERNHYSAQVILLHAAPGFTIRANFWPAAADSVVV
                     NSGTEPFFYGVPHDHNFSFLTVGYLGPGYWSDYFEYEYDEVSGFTGEEVPLRFVGRSR
                     LAEGRVMLYRKFRDVHSQLPPDRLSVTLNILAQSPAADFFDQYRFDLRRREIAGIINN
                     NPLEALLKLAVHLGGGNGRDLVDSFAERHPSERIRFAALEAQASSEPDREARVELYRR
                     AAGSGGFVGAMAARKARQLGSAPAELRL"
BASE COUNT         1169 a         2939 c         2788 g         1142 t
ORIGIN      
        1 cggccgccac cggcctccag tccaccgccg tcggcgacgt cgccagcgcg ctcgcgacca
       61 attcgagcgc cttcggccgc aacgccaatg tcgccgccgc cgccaccaac ggcacggcgc
      121 tcggcggcga gaccgtctcg ggcggcgtga actccaccgc cgtcggctgg cgcgccgccg
      181 ccaccggcgc ctcgtcgacc gccctcggcg accgcgccgt cgcctcgggc aacttctcga
      241 ccgcgctcgg cgatttcgcc gcggccaccg gcctccagtc gaccgccgtc ggcgacgtcg
      301 ccagcgcgct cgcgaccaac ggcagcgcct tcggccgcaa cgccaacgtc gccgccgcgg
      361 cgaccaacgg caccgcgctc ggcagcgagt ccgccgcggg cggagtcaac gccaccgccg
      421 tcggctggcg cacttcggcg accggccagt cgtcgaccgc tctcggcgac cgcgccgtcg
      481 cctcgggcaa cttctcgacc gccctcggcg atttcgccgc cgccaccggc ctccagtcca
      541 ccgccgtcgg cgacgtggcc agcgctctgg gcgccaattc gaccgcggtc ggccgtgacg
      601 ccaacgcgac ggcggcgaac tcgacggcga tcggcaacgc cgctcgggcg acccatgcca
      661 actcgacggc gatcggcaac ggcgccacca cgacggcggc caaccaggtg acggtcggcg
      721 gcgccggctc gacggtccgc ttcggcggct acaccacggc gggcgtgctg gtgaacgacg
      781 ccaacggcaa catcagcacc aacaccaccc tgctcggcca ggtggcgacc aacaccgcca
      841 acatcaccac cctgcagggc aacgtcacca acctgcagac gctgaccgcc acccacacga
      901 cgcagatcgc cgacctgttc gacctgtcgg acatcaaccg ccgcgacatc cgcaaggcca
      961 atgaaggcgt cgccatggcg ctggcgatgg agactcccca ccttccggcc ggcaccacca
     1021 tgggcgtcgc cggcggcatc ggctattacc agaaccggct cgccggcacg gcgtccttcg
     1081 cggcccgggt gggcacgaac gcgtcgttct cggccggcgt cggcctcggc ttcgacagcg
     1141 gcgagatcgg cgcccgcgcc gggttccaga ccgcgtggta aagctgcgct ttcgcgcaaa
     1201 ggtcgggccg gggttcttca ccccggcccg gctttttttt gccgcctgcc tggccgcgtc
     1261 cgccgcgccg gcggcggcgc aggcgcccgc gcctgcgccg atcgcgaatc aggccggggt
     1321 ccccgatccg atcgtcgtca acaagctggt ctggtcggcc atggccgcgc tcgaccaggc
     1381 gaaccagacc ggcaattatt cggtcctgcg cgatctcggg gcgccgagct tccagacgaa
     1441 caattcggcc gcgactctgg gcggcatctt ccaggcgctg cgggcgcagc aggtcgatct
     1501 cggctacacc ctcctggtgg cgccgagctt ccaattcccc cccgcgatcg tccagggcgg
     1561 cctgcttcga gtccgcggaa ccttcccgct gcggccggcg gcgatcggat tcgacctgct
     1621 ttttcagaac atttccggcc agtggcgcat ttttggcatc gcggtcgcgc ctattgtggc
     1681 ccaggcgcca caatccggct cgacaaggcg ttgaaaaaca ggctaaacgc gcaccgatct
     1741 tgcaactttc ccgagtctca ctaacaatcg aatcagcagg gagatacatc aatgcaccag
     1801 aacgttaacc agactgcggc tccgaagcgc gattgggctc agccccaggt tcggcgcctc
     1861 gaagccggcg cggcggagag ccagcgaggg gcgacgccgg acggcggcgg cggcaaccag
     1921 ggctcgtaag tcctgtcgcg acgctgtttc taagccccct gcctcagggc ggggggcttt
     1981 gtcatgaccg caatcgcggg cttctggagc ttcgacggcc ggttcgatcc gtccgcgcag
     2041 tgcgaggcga tgatcgcggc gcagtcgatc tacggcttgc gccagtccgc cgcccgcctc
     2101 gaccggctgg ccctcgccgc ccggctctat cccctccttc cggaagacgc acatgaccgg
     2161 cagccgctgg tcggcggcga cgggcgtttc gcgctcgtcg ccgacgtgcg tctcgacaac
     2221 cgggccgagc tcgccggcgc actgggcctg gcgcccgccg agaccgcgtc cctcgccgat
     2281 tcggatctgc tgctgcgcgc gctcgagcgc tggggagaag atgcggtcgc aaggctcctg
     2341 ggcgatttcg ccttcgcctt ctacgacgcc cgcgaagcga cgttgctgct cgcccgcgat
     2401 ccgctcggcc agcggcccct gttctggcac cgaggcgccg gattggccgc tttcgcctcg
     2461 atgcccgccg ggcttcacgc cctcgccgcg gtgccgcgcc gccgcgacga gaccagcctg
     2521 atccgctatc tcgcctccct ccccccgctc ggcccgagca gcttccacga gggcatcgag
     2581 cgggtcgagc cggggcattt ggtcaccctc accccttcgc ggacgatccg ccggcgccat
     2641 tggcggccgc aacgccgaga cctggggctc aggcggttcg acgattatgt cgaggcctgg
     2701 cgctcggagc tggacgcggc cgtcgcgcgc cggctgcgcg gcgccggcgg cctcgtcgcc
     2761 gcccatctga gcggcggctg ggacagcaac gcggtcgccg ccacggccgc gcggctgacg
     2821 gcggccggcg gcggccgggt ggtcgccttc acccatgtcc cccgccccga ggctcgggcc
     2881 ggctcgccgt ccaaccgctt cgccgacgaa ggcgccctcg ccgccgccac cgcccgcctc
     2941 caccccaata tggaccattc gatcctgccg agctcgtgcc gctcgccgct ggcggacctg
     3001 gaccgctacg cggcgctgtt cgaccgcccg gtcttcaacc tgtgcaatca cgtctggctg
     3061 tccgagatcc gggccgccgc gagcgcggcc ggcgcgcgag tgctgctcag cggcgagatc
     3121 ggcaattgga cgatcagcgc cgcgcccaac gcgctcctcg cggatttcct gcgggaaggg
     3181 cggctgatcg cctgggcgcg ggcggcgcgc ggcctggtgc gcaccggccg ggcgcgctgg
     3241 cgcggagtcg ccgccagctc gttcggtgcc tgggtccccg attcggcgtg gcggcggctc
     3301 tcgggcctgg cgtccggacc cgaggcgtcg agcaccgacc ctgtccaccc tcagcttcgc
     3361 gacgcggtag aggcggcgcg gcaggcggac ggcggcggga tcgagcggcg caggaccgcg
     3421 ttcgaggacg ccgccgacgc cctgcaggcg atggactggg gcgagcatcg caagggcatc
     3481 ctcggcggat ggggcctcga caagcgcgac ccgacggccg acgtccggct ggtcgatttc
     3541 tgcctctcgc tgccgctcga catgctgatg aaggacggcg tccggcggcc gctggcgcgg
     3601 gccgcgctcg ccgaccgggt cgccccggcc gtcctcgacg agcgccgcag gggctatcag
     3661 tcggcggatt ggcacgtcgg cctgacccgc gatcgcgcca cggccgtccg cctgctcggc
     3721 gccatggcgg acgactccgc cgcttcgcgc ctgatcgacg tcgagcggct gcagcgcctg
     3781 gtccgcgact ggccggacgg cggctgggag agcgcccgga cgatcggagc ctatcggatc
     3841 gggctcctca agggacttac cgccggacac ttcatcttgg cagccggccg ctgatcctcc
     3901 tatggaacgc ccggtgcggg acagtcagtc agaacggcga gaacgatcgg atagcacatg
     3961 ctagcgcccg agggcgcgcg ccgcgcctcg acctatcggc tgttcggcct cgacctcagg
     4021 tcggagatcg accttgccgg gctcgccccc accgtcgatt ccggatcgcc cgacgtcgag
     4081 atcgcgttcg gcgccgtccc cgccagcgat cataacggct atcgcccgac cgaggacggc
     4141 accttgctgg cggtggcgaa ggtcggccgc tacctgatcc gcgagggtcg ccagatcctg
     4201 atcgacccgg cccccgacgc ctcggagcgc aacgtccggc tcttcctcct gggctctgcg
     4261 atcggggcgc tgctccacca gcgcggtctg cttcccctcc atgccaatgc gatcgacctc
     4321 ggcgggcggg cggtggcctt ttccggccat tcgggggccg gcaagtcgac catcgcggcc
     4381 tggttccacg atcggggcca ccggatcctg gccgacgacg tctgcgtgat cggcttcgac
     4441 gcggccgggc gcgcgctcgc ctatcccggg attccgcggc tgcggctgtg gcgcgaggcg
     4501 ctcgaggcga gcggccgcga cgcggacgcc tattcgcgct ccttcgacga caccgacaaa
     4561 tatgacgtgc cgacggcgaa cgaaggcggg ctcgacccga ttcccctcgc cgctatctat
     4621 ctgctccgca aggcggagga ggaggcgggc gaagccgaaa tcgagcggct gaccggcgtc
     4681 gacgcggtcg aaaccctggt ctccaacacc tatcgcggcg gctatctgag catgatcggg
     4741 cggaccagcc agcatctggc ggcctgcatc agggtcgccc gagcggtgcc ggtgttccgc
     4801 gcccagcgcc tttgggggtt cgagcggttc gacgagcagg cgggtcggct gcgcgaccat
     4861 gccagcgcgc agcttgtgca tggataatcg ccggcgcagc gtctaggcgg accccgccag
     4921 gaccaacaag gggagccggc cttgtccgga tacgagctga tctcagggat cgaggtggac
     4981 cgcgggctga aggcgttcgt tgacgaggaa gcccttccgg ggaccgggat cgacccggcg
     5041 gcattctggt tcggcttctc ggcgctgctg cgcgagctca cccccgagaa caagcggctg
     5101 ctgcgcaagc gcgaggacct ccaggctcga atcgacgccc gcaaccggag cctgggcggc
     5161 cggccggccg atccggcgga ggaagaggag ttcctgcgcg agatcggcta cctcgtcgat
     5221 ccgccggcgc gcttcgcgat cgcgacgagc gacgtcgatc cggagatcgc ccggatcgcc
     5281 ggcccgcagc tggtcgtgcc ggtgagcaac gcgcgctacg cgctcaacgc cgccaatgcc
     5341 cgctggggca gcctctacga cgcgctctac ggcaccgacg cgctgggcga ccggccggcc
     5401 tcgggcggct acgatgccga gcggggcgcg cgcgtggtcg cctggggccg cgccttcctc
     5461 gacgagatcg ccccgctgac cggggggagc cacgccgacg tcgccgccta ccgcatcgag
     5521 gattcgcgcc tggtcacgga ccgcggaacc ctccgcgacc cggcgctgct ggccggctgg
     5581 cgcggcgaat cgccgctgct tcgccaccat gggctccacg tcgagatcct cgtcgaccgc
     5641 gaccacccga tcggtcgcgg cgaccgggcg ggggtcgccg acatcgtgct cgaaagcgcg
     5701 gtcacggcga tcatggactg cgaggattcg gtggccgcgg tcgacgccga ggagaaggtg
     5761 ggagtctatc gcaactggct cgggctgatg aagggcacgc tcagcgccgg cttcgacaag
     5821 ggcggccgaa gcgtcgaccg gcggctggcg ccggaccggg tcttcgaggc cccgggcggc
     5881 ggcacgctga cgctcaaggg ccgagccctg atgttcgtgc gcaacgtcgg ccacctgatg
     5941 gccaacccga tgatgaggat cgacggggag gacgtccgcg aggggctggt ggacgcggtg
     6001 atcaccggcc tcgccgccct gcacgacctc aacggcggac gcgccaacag cccggccgga
     6061 tcggtctaca tcgtcaagcc gaagatgcac gggccggagg aggccgcctt cgccggccgg
     6121 ctcttcgacc gcaccgagga tctgctcgga ctgccccgcc acagcctcaa gatcggcgtg
     6181 atggacgagg agcgccggac cagcgtcaac ctcgcggcgg tgatccacgc ggtgaaggac
     6241 cgggtggtct tcatcaacac cggcttcctc gaccgcaccg gcgacgagat ccacacggcg
     6301 atgcgtctgg ggccggtcat ccgcaaggcg gagatgaaga gctccgcctg gctcaaggcc
     6361 tatgaggaca ataatgtcga cgtcggcctt gcctgcggct tcgccggccg ggcccagatc
     6421 ggcaagggca tgtgggcgat gccggacctg atggccgaca tgctcgccca gaagatcgcc
     6481 catccgcagg ccggagcgac caccgcctgg gtcccctccc ccaccgccgc gaccctccac
     6541 gccgtccact accaccgggt cgacgtggcc gagcggcagc gggagatcgc agcccggccc
     6601 gccgcccccc tcgagggcct gctcgccgtt ccggtcgccg tcgagcccgg ctggtcggcc
     6661 gacgccgtgt gcgaggagct ggacaacaac gtccagggca tactcggcta tgtggtgcgc
     6721 tggatcgacc agggcgtcgg ctgctcgaag gtgcccgaca tccacgacgt cggactgatg
     6781 gaggatcggg ccacctgccg gatctccagc cagcatgtcg ccaattggat gatgcacggc
     6841 gtggtcagcg aagcgcaggt cgaggaagcg ctccggcgga tggccgcggt cgtcgaccgc
     6901 cagaacgcga acgacccggc ctatcgtccg atggccgccg atttcgagcg aagcaccgcc
     6961 ttccgcgccg ctcgggcgct ggtcctccag ggggcggatc agccgtcggg ctataccgag
     7021 cccctgctcc acgcgcatcg gctggcgcta aaggcggagc tcggccggag ctgagccgag
     7081 ctggcgggcc ttgcgggccg ccatcgcccc gacgaacccg ccggagccgg cggcccgccg
     7141 gtagagctcg acccgggcct cgcggtcggg ctcgctcgag gcctgcgcct ccagggcggc
     7201 gaagcggatg cgctcgctgg ggtggcgctc ggcgaagctg tcgaccaggt cgcggccatt
     7261 gccgccgccg agatggacgg ccagcttgag cagcgcctcg agcgggttgt tgttgatgat
     7321 tccggcaatc tcccgccggc gcaggtcgaa cctgtactgg tcgaagaaat cggccgccgg
     7381 agactgggcc aggatgttga gggtgaccga cagccggtcg ggcggcagct gcgaatggac
     7441 gtcccggaac ttgcggtaga gcatcaccct cccctcggcg agccgcgacc gcccgacgaa
     7501 gcgcagcggg acttcctcgc cggtgaagcc ggacacctcg tcatattcat attcgaaata
     7561 gtcgctccaa tagcccggac cgagatagcc gacggtcagg aagctgaaat tatggtcgtg
     7621 gggcacgccg tagaaaaagg gttccgtgcc gctgttgacg accacgctgt cggccgccgc
     7681 gggccagaaa ttggcgcgga tcgtgaagcc cggcgcggca tggaggagga tcacctgcgc
     7741 ggagtaatgg ttgcgctcca cctgaccggc gcagctgtgc ttgagctcct caacgacgag
     7801 gtcgccgagg aagctccggt tgttgccgag cctgctcagc gccggcgcga aggcggcgag
     7861 gctggaatcg tcgtcggggt cgaatccgcc cgtcgccagc gcctcgacga ggtcgccgag
     7921 ctggatcgcc tcggcggtgt cggtatcgat cacacggggc atggcaggca ggcttccggg
     7981 aagaatgcgg agagggtctc ggcggccgcc gcgcgcacgt ccggatgggg atccgatc
//
LOCUS       DASVQZ010000340         4381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_938582, whole genome shotgun sequence.
ACCESSION   DASVQZ010000340 DASVQZ010000000
VERSION     DASVQZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938582"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VF547_09700"
     CDS             complement(<1..1516)
                     /locus_tag="VF547_09700"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX8263133.1"
                     /translation="MRKGRFAGLSDKGQEPVSPPGLAELLAFDFAPAGNEDDDRAACE
                     RGRTVQLSLPLLTGSHLLWAAVLLGALGGAAPTPLAALLAAVLLLDGLLWGALKRFAL
                     KPHRAMRLAAAHGAAAGGLWAAAAWLLAAHPAGESVVVKAALIAGAGAAIPVFFTIPL
                     LMALASAATLATAALLPSDEPLVPIGAAIGALLLSLCVSRSRHLLFAARQRLALEWQA
                     EKARRFVADFEASGRGWFWETNADGALTYVSEPLAVHLGLAAAGLIGRRFDELLLPDG
                     GDEDLDDSRRTLGFHLSARFPFADVVVRAPAGEDVWWSLSGNPNFDEYGRFLGFRGLG
                     TNLSEQRRSEAETSKRARYDSLTNLPNRAMMRDMLDSALANADARRRGCALMMIDLDR
                     FKQVNDRLGHPVGDKLLRKVSGRLTEVLGEEGQVGRLGGDEFEAILPGIEEEGRLSEL
                     AGRLIEQVSRPYKIEGHEIRIGTSVGIAVALPGKTLAQSLIKDADVALYAAKRDG"
     gene            complement(1616..2053)
                     /locus_tag="VF547_09705"
     CDS             complement(1616..2053)
                     /locus_tag="VF547_09705"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8263134.1"
                     /translation="MSSDDDNGVDSGRKAERLTLDAEARLRPNSWSSLQIRMLDLSAS
                     GFRAECEARVRPGGSVSLDVPGIGAVEAQVEWQRGSQFGARFFAPIELASCQWTFRER
                     HHALARLLVERAAARRAGRRGAEGQLRRDILSALPMRKGCASA"
     gene            complement(2145..>4381)
                     /locus_tag="VF547_09710"
     CDS             complement(2145..>4381)
                     /locus_tag="VF547_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX8263135.1"
                     /translation="PKDRPALIQVAAILVSSTRASGALRSDPGGFTGRLDVDGGGLAG
                     RLVFDPWQGIQRIAIDLKADNARFAGPPPILIRSGRLEGVALLDPAGTSLRGTLNARG
                     LSRGALSIASLGATGELRGGTGQIRARIAGTRGRDFVFDTVADIAPGRYRLTGRGTVD
                     RRAIVLSEPALLTREGDAWRLAPTALTFAGGNATVSGLFGGSTSELNARVERMPLGVL
                     DIGWPALGLGGTASGTLSYRFGAVGEPSGDADLRVRGLTRSGLVLSSKPVDIGIVARL
                     VGGNAALRAVAASDGRTIGRAQARLAPIGRSGNIGERLAAAPLFAQLRYNGPADTLWR
                     LTGVELLDVSGPVAVGADARGTLANPQIRGSLRTERARIESAVTGMVIDNVRASGRFG
                     GSKLVLDSFAGTTKRGGTVSGRATFDLAGPKGFGIDIALDAKAAQLLDRDDIKAQVTG
                     PVAIRSDGENGSISGKVDLVSGSFRLGSVTASTQVPRLAVRELNRPADERPVARRLSP
                     WRLDLDVRARNRLMVAGLGINSEWSADLRIAGTVTEPRISGRADLIRGSYDFAGRRFD
                     LERGQIRFLGESPVNPVLDITAEGGVQGLNAVIRVTGRGQRPEISFTSTPALPQDELL
                     SRLLFGTSITNLSAPEALQLAAAVASLNDSGGGLDPINAVRAATGLDRLRIVPADITT
                     GQGTAIAAGKYLGRRVYVEVVTDARGYSATMVEYQITRWLSILSSISTIGRESVNVRV
                     SKDY"
BASE COUNT          616 a         1561 c         1616 g          588 t
ORIGIN      
        1 ggccgtcgcg cttggcggcg tagagcgcga cgtcggcgtc cttgatcagg ctttgcgcca
       61 acgtcttgcc gggcaaagcg acggcgatgc cgacggaggt gccgatgcgg atctcgtgcc
      121 cctcgatctt gtacgggcgc gagacctgct cgatcagccg cccggccagc tccgacagcc
      181 ggccctcctc ctcgattccg ggcaggatcg cctcgaactc gtcgccgccg aggcgcccga
      241 cctggccctc ttcgcccaga acctcggtca gccggcccga gaccttccgg agcagcttgt
      301 cgccgaccgg gtggccgagc cggtcgttga cctgcttgaa ccggtccagg tcgatcatca
      361 tcagcgcgca gccgcgccgc ctcgcatccg cattggccag ggcggagtcg agcatgtcgc
      421 gcatcatcgc ccggttgggc aggttggtga ggctgtcgta gcgggcgcgc ttcgaggtct
      481 cggcctcgct ccggcgctgc tcgctgaggt tggtgccgag gccccggaag ccgaggaacc
      541 ggccatattc gtcgaaattg ggattgccgg acagcgacca ccagacgtcc tcgccggcgg
      601 gcgcgcggac caccacgtcg gcgaacggga agcgcgccga gaggtggaag ccgagcgtcc
      661 ggcggctgtc gtccaaatcc tcgtcgccgc catcgggcag cagcagctcg tcgaaacgcc
      721 ggccgatcag gccggcggcg gcgaggccga gatggacggc gagcggctcg gagacgtagg
      781 tcagcgctcc gtcggcattg gtctcccaga accagccgcg cccgctcgcc tcgaaatcgg
      841 cgacgaagcg cctcgccttc tccgcctgcc actcgagcgc gaggcgctgc cgcgcggcga
      901 acaggagatg gcgcgagcgc gacacgcaca gcgacaggag cagcgcgccg atcgccgccc
      961 cgatcggcac gagcggctcg tcgctgggca gaagggcggc ggtcgcgaga gtggcggcgc
     1021 tggcgagcgc catcagcagg ggaatggtga agaagaccgg aatggccgcg cccgcgcccg
     1081 cgatcagcgc cgccttgacg acgaccgact caccggccgg gtgggcggcg aggagccacg
     1141 ccgccgcggc ccatagcccg ccggcggcgg cgccgtgcgc ggcggcgagc cgcatcgccc
     1201 ggtgcggctt cagagcgaag cgcttcaggg ctccccagag caggccgtcg agcagcagca
     1261 cggccgcgag cagggcggcc agcggcgtgg gcgcggcccc gccgagcgct ccgagcagca
     1321 ccgccgccca aagcaaatgc gagccggtca gcagcggcag cgagagctgg acggttcggc
     1381 cgcgctcgca ggcggcgcga tcgtcgtcct cgttgccggc gggggcgaaa tcgaacgcca
     1441 gcagctccgc gagccccggc ggagagacgg gctcctggcc cttgtcgctc aaaccggcga
     1501 accgcccttt gcgcatcgcg tcagcgtccc gataatgatc cgccggttcc ccccgcaggc
     1561 cggcagcggg aacctaggat aacccaagtt aagaaagcgc ttatcgcgcc ccgattcatg
     1621 ccgacgcgca gcccttgcgc atcggcaagg ccgacaggat gtcgcgccga agctggcctt
     1681 ccgcgccacg acggccggcc cgcctggcgg cggcgcgctc gaccagcagc cgcgccaggg
     1741 catggtggcg ctcgcggaag gtccactggc agctcgcgag ctcgatcggg gcgaagaagc
     1801 gggcgccgaa ctggcttccg cgctgccatt ccacctgcgc ctcgacggcg ccgatgccgg
     1861 gcacgtcgag cgacacgctg ccgcccggcc ggacccgcgc ctcgcattcg gcgcggaagc
     1921 ccgacgccga caggtcgagc atccgtatct ggaggctgct ccagctgttg ggcctgagcc
     1981 gcgcttccgc gtcgagcgtc aggcgttccg ccttgcggcc gctgtcgacg ccgttgtcgt
     2041 cgtccgagga cattcgatcc ccatccccaa gttgaggtcg gggtgagact agcgcctcgg
     2101 cgttaacgaa ttgctgccgc tcgtccgcac ggccgcccgg actctcaata atccttcgac
     2161 accctgacgt tgacgctctc gcggccgatg gtcgagatgc tggagaggat cgagagccag
     2221 cgcgtgatct gatattcgac catcgtcgcc gaatagccgc gggcgtcggt gaccacctcc
     2281 acatagaccc gtcggccgag atacttgccg gcggcgatgg cggtgccctg gccggtggtg
     2341 atgtcggcgg ggacgatgcg caggcggtcg aggccggtgg cggcgcggac ggcgttgatc
     2401 gggtcgaggc cgcccccgct gtcgttcagc gacgcgaccg cggcggcgag ctgcagcgcc
     2461 tcgggcgccg acaggttggt gatcgaggtg ccgaacagca gccggctgag cagctcgtcc
     2521 tgaggcaggg ccggcgtgct ggtgaaggat atttcgggcc gctggccgcg gccggtgacc
     2581 cggatgacgg cgttgaggcc ctggaccccg ccctcggcgg tgatgtcgag caccgggttg
     2641 accggggatt cgccgaggaa gcggatctgg ccccgctcga ggtcgaagcg gcggccggcg
     2701 aaatcgtagc tgccgcggat gaggtcggcc cggccgctga tccgcggctc ggtgacggtt
     2761 ccggcgatcc tgaggtcggc gctccattcg ctgttgatgc cgaggccggc gaccatcagc
     2821 cggttgcgcg ccctgacgtc gaggtccagc cgccacggcg acaggcgccg ggcgacggga
     2881 cgctcgtcgg ccggacggtt gagctcgcgc acggcgagcc gcggcacctg ggtcgaggcg
     2941 gtgacgctgc ccagccggaa gcttccgctg acgaggtcga ccttgccgga gatgcttccg
     3001 ttctcgccgt ccgagcggat cgcgaccggg ccggtcacct gcgccttgat gtcgtcgcgg
     3061 tcgagcagct gcgccgcctt ggcgtcgagc gctatgtcga ttccgaagcc cttcgggccg
     3121 gcgaggtcga acgtggcgcg cccgctcacc gtgccgccgc gcttggtggt gcccgcgaag
     3181 ctgtcgagca ccagcttgga gccgccgaac cgtccggagg cgcggacatt gtcgatgacc
     3241 atgccggtca cggcgctctc gatccgcgcc cgctcggtgc gaagcgaccc gcggatctgc
     3301 ggattggcca gcgtgccgcg cgcgtcggcg ccgaccgcca ccgggccgga cacgtcgagg
     3361 agctccactc cggtcagccg ccacaaggtg tcggccgggc cgttgtagcg cagctgcgcg
     3421 aacagcggcg cggcggcgag gcgctcgccg atattgccgc tccgcccgat cggcgccagc
     3481 cgcgcctgcg cccggccgat cgtccggccg tcgctcgccg ccaccgcgcg aagcgccgcg
     3541 ttgccgccca cgagcctggc gacgatgccg atgtcgaccg gcttggagga gaggacgagg
     3601 cccgagcggg tgaggccgcg cacgcgcaaa tcggcgtccc cgctcggctc gccgacggcg
     3661 ccgaagcggt agctgagcgt gcccgaggcg gtgccgccga ggcccagggc cggccagccg
     3721 atgtcgagca ctccgagcgg catccgttcg actcgcgcat tcaattcgct ggtcgagccg
     3781 ccgaacaggc ccgagacggt ggcgttgccg ccggcgaagg tgagggcggt gggggccagc
     3841 cgccaggcgt cgccttcgcg cgtcagcagc gccggctcgc tcagtacgat cgctcggcgg
     3901 tcgacggtgc cgcgcccggt gaggcggtag cggccgggcg cgatgtcggc gaccgtatcg
     3961 aagacgaagt cgcgtccgcg cgtgccggcg atccgggccc ggatctgccc cgtcccgccg
     4021 cgcagctcgc cggtggcgcc gaggctggcg atcgacagcg cgccgcggct cagcccgcgc
     4081 gcgttcagag tcccccgcag cgaggtcccg gccgggtcga gcagggcgac cccctcgagc
     4141 cggccgcttc ggatcaggat cggcggcggg ccggcgaagc gggcgttgtc cgccttgagg
     4201 tcgatcgcga tccgctggat gccctgccaa gggtcgaaca ccagccggcc ggcgaggccg
     4261 cccccgtcga cgtcgagccg tccggtgaac ccgcccggat cggagcgcag ggctccggag
     4321 gctcgggtgc tcgacacgag gatcgccgcg acctggatca gggccggccg gtccttcggc
     4381 a
//
LOCUS       DASVQZ010000341        12723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_841081, whole genome shotgun sequence.
ACCESSION   DASVQZ010000341 DASVQZ010000000
VERSION     DASVQZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12723
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841081"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1656
                     /locus_tag="VF547_09715"
     CDS             160..1656
                     /locus_tag="VF547_09715"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX8263136.1"
                     /translation="MRNIDHYIGGGAHASGERQGDVFDPNSGGVQARVRFGTAADLAR
                     AVENANAALPGWAATNPQRRARVMFNFKALVEKNMEDLALLLSSEHGKVLADARGDIQ
                     RGLEVIEFACGIPHALKGEYTQGAGPGIDVYSMRQPLGIVAGITPFNFPAMIPMWMFG
                     VALACGNAFILKPSERDPSVPVRLAELMKEAGLPDGILQVVHGDKEMVDAILDHDDIK
                     AISFVGSSDIAQYIYSRGTANGKRVQAFGGAKNHGIVMPDADLDMVVNDLAGAAFGSA
                     GERCMALPVVVPVGDKTAANLREKLIPAIEGLRVGVSTDAEAHYGPVVNAAHRSRIEN
                     YIQMCADEGGELVVDGRGFSLQGHEEGFFIGPTFFDHVKPHFRSYQQEIFGPVLQMVR
                     ADSFEEALRLPSEHQYGNGVAIFTRNGHAAREFAARVNVGMVGINVPIPVPVAYHTFG
                     GWKRSGFGDINQHGMEGVRFWTKTKTVTQRWPDGSVTGENAFVIPTMG"
     gene            1660..2133
                     /locus_tag="VF547_09720"
     CDS             1660..2133
                     /locus_tag="VF547_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263137.1"
                     /translation="MIAGLILALLAAPEAAAEPAQAESWAGPGRYCGYATEIDLAEGE
                     TISTRRLGIHGAAFEWMGKFGRIEVSEINWASRPVGRPVHIEGIDRARVVRVTRRGAG
                     PTYALWNENHLAAYLTGPALRNQAAVAGILRRIELVDHAGERSEGCKYRTLFSWE"
     gene            2130..3107
                     /locus_tag="VF547_09725"
     CDS             2130..3107
                     /locus_tag="VF547_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8263138.1"
                     /translation="MKAAAAAVRPLLLVLLLLAFASSAVASPPQKRIALSFDDVPRRP
                     GAFLTPDQRTQGILSGLAAAKVEQAVFFVTTGNLDKPEMKGGEARIMMYSGAGHLIAN
                     HSHAHRRLSQVAAEAYLEDIDRASAWLRNRPGSRPWFRFPFLDEGRADKAKRDAVRAG
                     LAARRLANGYVTVDGADWNMEALTVAAHEAGKHMDMKALRELYVETMVGAANFYDGLA
                     RRTLGRSPAHMLLLHETDLAALFLPALVEALRKDGWKIITADEAYADPIAEAAPDTPS
                     AQGTRIEAMAWEKGLPAPRWYERENLKIARGLFAERVLHEPPVDPDAKQ"
     gene            3155..3451
                     /locus_tag="VF547_09730"
     CDS             3155..3451
                     /locus_tag="VF547_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263139.1"
                     /translation="MNARVAFDTHAFVKRLEAAGLSVPQAEALADAMGDIVLQSIATK
                     SDLREVEVNLRSEIKDLGTALRGEMKDMELRLTLRMAAMFAAMVAILAGLKIFS"
     gene            3496..4638
                     /locus_tag="VF547_09735"
     CDS             3496..4638
                     /locus_tag="VF547_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8263140.1"
                     /translation="MSQFDLTDEQRQIQEMARSFTADAITPHAAEWDEKHIFPRDVIR
                     QAAELGFGSIYVSEESGGIGLGRLEAALIMEAMAYGCPSTSAFISIHNMASWMIDRFG
                     SAAVKEKYLPSLIPMERMASYCLTEPSSGSDAAALKTKAVKDGDSYVVSGSKAFISGG
                     GENEVYVTMVRTGQEGPKGISCLVIEKDMPGVSFGAQERKLGWHSQPTAQVNFDDVRV
                     PAENLVGGEGEGFRIAMMGLDGGRLNIGACSLGGAQRCLDEAIAYTKERKQFGTPIAD
                     FQATQFTLADMETELQAARMLLYAAAVKVTENAPDKSRFAAMAKRLATDTGSSVVDRA
                     LQLHGGYGYLQDYPVERFWRDLRVHSILEGTNQIMRVIVARDMLRQ"
     gene            4765..5808
                     /locus_tag="VF547_09740"
     CDS             4765..5808
                     /locus_tag="VF547_09740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8263141.1"
                     /translation="MTPDLITRVEGKVGRLRLNRPKAIHALTKEMCSAMIAALLPWRE
                     DPSVHAVMIDHAEGRGFCAGGDVVMLARSGAGDGAEARAFFHEEYRLNHLLFTYAKPT
                     VAFMDGITMGGGVGISQPCRYRVATENTRFAMPETAIGLFPDVGGGWYLPRLPGRTGH
                     FLALTGARLDGSECLRLGLATHYIPSLALDDLKARMLAEPERLEALLEENSATPPPAR
                     IEANLPLIDRLFASDDYEDVLAALEADGSDWARTELETLRGKSPQACKVSLRLLYDGA
                     RVQDFAHEMKQEYAVATRVVQRHDFVEGVRALLVDKDNSPRWEPSTPEGVTQHLIDTI
                     FAPMPEGEEWTPL"
     gene            5928..6701
                     /locus_tag="VF547_09745"
     CDS             5928..6701
                     /locus_tag="VF547_09745"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8263142.1"
                     /translation="MNYENILVEQRGAVTLITLNRPKALNALNTAVLAELIAAFAAYD
                     SDGSQRCAVLTGSERAFAAGADIKEMSDQGFASMYSSNFFQGWERVTATRKPWIAAVA
                     GYALGGGCEVAMMADFIIAADTAQFGQPEIKLGVTPGMGGSQRLTRAVGKAKAMEMCL
                     TGRMMGAEEAERSGLVARVVPAAELLDEAMKAAEAIASMAPLAAIATKEMVNAAFETG
                     LAQGIVFERRLFHGLFGTQDQKEGMAAFVDKRKAEWKGR"
     gene            6847..7743
                     /gene="mmsB"
                     /locus_tag="VF547_09750"
     CDS             6847..7743
                     /gene="mmsB"
                     /locus_tag="VF547_09750"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX8263143.1"
                     /translation="MARIAFIGLGNMGGGMAANLVKAGHEVRAFDLSEAALAKAESNG
                     CARAGSAAEAVADAEAVVTMLPAGKHVRDVYELSVIGTAPAGAILIDCSTIDVATARE
                     EIAKAAAAGYAMVDAPVSGGIAAAEAGTLTFMVGGSDEGFERAREILEKMGKAVIHAG
                     GPGSGQAAKICNNMLLGASMVATCETFVLAEKLGLDPQTFFDIASKASGQCWSMTTYC
                     PVPGVGPVTPADRDYEGGFASALMLKDLRLAMEAAQSEDAYTPMGAHAEELYTRFAEA
                     LGGGRKDFSGIIKMIDDSWEAS"
     gene            complement(7846..9036)
                     /gene="wecB"
                     /locus_tag="VF547_09755"
     CDS             complement(7846..9036)
                     /gene="wecB"
                     /locus_tag="VF547_09755"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX8263144.1"
                     /translation="MAQRVHLIAAARPNFMKVAPLWHALAAAPGFEPVLIHTGQHYDV
                     NMSDAFFADLRLPEPDHHLGIGSGSHAAQTGRVMIAYEQVAVADRPDWLVVVGDVNST
                     AACAMAGAKLGIPVIHLEAGLRSRDRAMPEELNRLVTDVLADVLWTPSPDADENLVAE
                     GIPTGRITRIGNIMLDSFELVRPAIEAADVPGELGLERGGYGVVTLHRPSNVDDPAQL
                     IRLVDALVETQRRLPLVFPVHPRTARRLAGALFDGRLARAGVRLIEPLPYVRFMSLVT
                     GAAAAITDSGGIQEETTYLGIPCLTLRENTERPVTITEGTNRLVTPETLLIELERALT
                     GPRALRCPELWDGKAAARCVDDLRRRSAIVKLPVAEPGPAELAARYGGGERRRAGASY
                     RRSS"
     gene            complement(9062..10594)
                     /gene="glpK"
                     /locus_tag="VF547_09760"
     CDS             complement(9062..10594)
                     /gene="glpK"
                     /locus_tag="VF547_09760"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX8263145.1"
                     /translation="MSEAMQADRPVSGDFILVIDEGTTSTRAMLFAADGSCLATEQRE
                     LVQSYPQPGWVEHDAEEIWASSLACARAVVAAGGGAGRVAALGITNQRETIVFWSRRT
                     GAALAPAIVWQDRRTAELCQTLKDRHHEGELREKTGLLLDPYFSGSKIAWALENWPQL
                     REAGDDLCVGTVESWLVFKLTGGLHVSDATNASRTALMHIGEGRWDDWLLDLWGVSRS
                     MLPEIVDCAGSFGTTLPEHFGLPIPIAGLAGDQQAASIGQACLWYGSTKATFGTGAFI
                     LANTGDELRVSQWRLLSTIAAQIGGQRTYALEGSIFVAGSLVKWLRDDLGLLASAAET
                     EALARSVPDSGGVTIMPAFVGLGAPFWLPNARGAIFGLTFATKRAHIARAALEAMAHQ
                     THSLMRGFAEDGAPWDVLNIDGGMASNDWLAQDLADILELPVERPRFVETTALGAAML
                     AGLGCGLFSSLEEASAMRGEVDRFEPNMAAEVRRERIRAWGRSLVRCEPNVIGQRAPT
                     LS"
     gene            10704..12179
                     /gene="glpD"
                     /locus_tag="VF547_09765"
     CDS             10704..12179
                     /gene="glpD"
                     /locus_tag="VF547_09765"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX8263146.1"
                     /translation="MGKQNFSIDLFDLLVVGGGINGAAIARDAAMRGASVLLVEKDDL
                     AAHTSSASSKLIHGGLRYLENYEFRLVREALAEREILLRTAPHIVRPLRFVLPHGPGL
                     RPHWMVRSGLLLYDLFSIRGSLPRSRRVGRREPELHAPLKERRRVLAYWDAWVDDSRL
                     VVLNAVAAAEHGAEIAVGTELLSARRSDGAWRAELSGGRTVAAKAIVNAAGPWVDEVL
                     SGRLAETSASRVRLVKGSHILGPALWQGDHGYILQQADGRVVFALPFKGDFTLIGTTD
                     VPVGRPEDAAAGPDEIRYLCEAADRYFTRQIVPADVVWSYSGIRALYDDGAADAKSVT
                     RDYHLELDPDPGPKLLSVFGGKLTTARALAEEALDRLGIEGRRSTSHSFLPGGDLSPG
                     FLEWLDGLAKWMPQPLLARLSGAYGTRLREMIGGARSLADLGRPFGAGLHEVELRWLR
                     DREFARTADDVLWRRTKLGLAMSQEEARAVEAWMRTPSLRA"
     gene            complement(12209..12334)
                     /locus_tag="VF547_09770"
     CDS             complement(12209..12334)
                     /locus_tag="VF547_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263147.1"
                     /translation="MGGDEEDLPSDDESIEQEAQKNREALDEVPPPGTDPLHEGP"
     gene            12407..>12723
                     /locus_tag="VF547_09775"
     CDS             12407..>12723
                     /locus_tag="VF547_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263148.1"
                     /translation="MAPSASEAAAAAAGKGGSVIGWKLDREQRKELLQQFPPRYPKVD
                     ADHVTLQAGAGADSALPDETLGEIVGRADDGRGVEAMVVAIGGTTERPDGSVYHITWS
                     LEPG"
BASE COUNT         2053 a         4444 c         4288 g         1938 t
ORIGIN      
        1 ccgtcacccc ggacttgatc cggggtccac cttcttcatc ggcgctttga aggaaaggtg
       61 gatgccggat caagtccggc atgacggctc cggctggcat tcctcgcggg gcttggctac
      121 gggctcgcca tccattccag agtcatccga gaaccatcca tgcgtaacat cgatcattat
      181 atcggcggcg gcgcccacgc ctccggcgag cggcagggcg acgtcttcga ccccaacagc
      241 ggcggcgtcc aggccagggt gcgcttcggg accgcggccg acctcgcccg ggcggtcgag
      301 aacgccaacg ccgccctccc cggctgggcc gcgaccaatc cgcagcgccg cgcccgggtc
      361 atgttcaact tcaaggcgct ggtcgagaag aacatggagg acctcgccct cctcctttcg
      421 tccgagcacg gcaaggtgct cgccgacgcc cgcggcgaca tccagcgcgg gctcgaggtc
      481 atcgagttcg cctgcggcat cccccatgcg ctgaagggcg aatataccca gggcgctggc
      541 cccggcatcg acgtctattc gatgcgccag ccgctcggaa tcgtcgccgg gatcaccccg
      601 ttcaacttcc cggcgatgat cccgatgtgg atgttcggag tggcgctggc ctgcggcaac
      661 gccttcatcc tcaagccctc cgagcgcgat ccctcggtcc ccgtccgcct cgccgagctg
      721 atgaaggagg cggggcttcc cgacggcatc ctccaggtcg tccatggcga caaggagatg
      781 gtcgacgcga tcctcgacca tgacgacatc aaggcgatca gcttcgtcgg ctcgtccgat
      841 atcgcccagt atatctacag ccgcggcacc gccaacggga agcgggtcca ggccttcggg
      901 ggcgccaaga accacggcat cgtcatgccc gacgccgatc tcgacatggt cgtcaacgac
      961 ctcgccggag ccgccttcgg atcggccggc gagcgctgca tggcgcttcc cgtcgtggtg
     1021 cccgtcggcg acaagaccgc cgcgaacctg cgcgagaagc tgattccggc gatcgagggg
     1081 ctgagggtcg gagtctcgac cgacgccgag gcccattacg gacccgtggt caacgccgcc
     1141 catcgcagcc ggatcgagaa ttacatccag atgtgcgccg acgagggcgg cgagctggtc
     1201 gtcgacggcc ggggcttctc gcttcagggc catgaggagg gcttcttcat cggcccgacc
     1261 ttcttcgacc atgtgaagcc gcatttccgc tcctaccagc aggagatttt cggccccgtg
     1321 ctgcaaatgg tccgcgccga cagcttcgag gaggctctcc gcctcccttc cgagcaccaa
     1381 tatggcaacg gagtcgccat cttcacccgc aacggccacg ccgcccgcga gttcgcggct
     1441 cgggtcaatg tcggaatggt cgggatcaac gtccccatcc ccgtccccgt cgcctaccac
     1501 accttcggcg gctggaagcg ctccggcttc ggcgacatca accagcacgg catggagggg
     1561 gtgcggttct ggaccaagac caagaccgtc acccagaggt ggccggacgg gtcggtgacc
     1621 ggcgagaacg ccttcgtcat cccgacgatg ggctagcgct tgatcgccgg gctgatcctc
     1681 gcgctgctcg cggctccgga ggcggcggcg gaaccggcgc aggcggagtc ctgggcgggc
     1741 cccgggcgct actgcggcta cgcgacggag atcgatctcg ccgaggggga aaccatctcg
     1801 acacggcgcc tcggaatcca tggcgccgcg ttcgaatgga tgggaaagtt cggcaggatc
     1861 gaggtcagcg agatcaactg ggcgtcgcgg ccggtcggca ggccggtgca catcgagggg
     1921 atcgaccgag cccgggtggt gagggtgacc cggcgcggcg ccggcccgac ctacgccttg
     1981 tggaacgaaa atcacctcgc cgcctatctg accggcccgg cgctcaggaa ccaggccgcg
     2041 gtcgccggga tattgcggcg gatcgagctc gtcgaccacg cgggcgagcg aagcgaaggc
     2101 tgcaaataca ggacactctt ctcgtgggaa tgaaagccgc agcagcggcc gtccgtccgc
     2161 tcctcctcgt cctgctgctc ttggccttcg cctcttccgc cgttgccagt ccgccgcaaa
     2221 agcgcatcgc gctcagcttc gacgacgtcc cccgccggcc cggcgccttc ctgacgcccg
     2281 accagcggac ccagggcata ctctccgggc tcgccgcggc caaggtggag caggcggtgt
     2341 tcttcgtcac caccggcaat ctcgacaagc cggagatgaa aggcggcgag gcgcggatca
     2401 tgatgtattc gggcgccggc catctcatcg ccaaccacag ccacgcccac cgccggctct
     2461 cgcaagtcgc tgccgaggcc tatctcgagg acatcgaccg cgcctccgca tggctccgca
     2521 atcggcccgg aagccggccc tggttccgct tcccgtttct cgacgagggt cgggccgaca
     2581 aggcgaagcg cgacgccgtc cgtgccggcc tggccgcccg ccgtctcgcc aacggctacg
     2641 tcaccgtcga cggggccgac tggaacatgg aggctctaac tgtcgccgcc catgaggccg
     2701 gcaagcatat ggacatgaag gcgctccgcg agctctacgt cgagacgatg gtgggcgcgg
     2761 ccaatttcta cgacggtctc gcccgccgga ccctcggccg ctcccccgcg cacatgctcc
     2821 tgctccacga gaccgacctc gccgccctgt tcctccccgc tctggtcgag gcgctgcgca
     2881 aggacggttg gaagatcatc accgccgacg aggcctatgc agaccctatc gcggaagccg
     2941 cgcccgacac gccgtcggcg caggggaccc ggatcgaggc gatggcctgg gaaaagggcc
     3001 tgccggcgcc ccggtggtac gagcgcgaga atttgaagat cgccagaggg ttgttcgcgg
     3061 agcgcgtgct tcacgaacct cccgttgatc ccgacgcaaa gcagtgaatg ttgtcgcgtt
     3121 ttgaacggaa aacgaaacgt gctatccttg tcggatgaac gctcgcgtcg ctttcgatac
     3181 ccatgccttc gtaaagcggc tagaagcggc gggactgagc gtcccccagg ccgaggcgct
     3241 cgccgacgcc atgggtgaca tcgtgctcca gtccatcgcc accaagtctg acctgcggga
     3301 ggtggaggtt aatctccgct ccgagatcaa ggacctgggt accgcgcttc gcggcgagat
     3361 gaaggatatg gaactccgtc tgacattgcg gatggcggca atgttcgccg cgatggtcgc
     3421 gatcctcgca ggcttgaaaa tctttagctg aaccctcccg attgcgattc ccgccgcgtc
     3481 cgctagagcg cgcgcatgag ccagttcgac ctcaccgacg agcagcgcca gatccaggag
     3541 atggcgcggt ccttcacagc cgacgcgatc acgccccatg cggccgagtg ggacgagaag
     3601 cacatcttcc cgcgcgacgt gatccgccag gcggctgaac tgggcttcgg ctcgatctac
     3661 gtctccgagg agagcggcgg gatcggcctc ggccgcctgg aggcggcgct gatcatggag
     3721 gcgatggcct atggctgccc gtcgacctcg gccttcatct cgatccacaa catggcgagc
     3781 tggatgatcg accgcttcgg ctcggcggcg gtgaaggaga agtacctgcc ctccctgatc
     3841 ccgatggagc ggatggcgag ctattgcctg accgagccct cgtccggctc cgacgcggcg
     3901 gcgctgaaga ccaaggccgt gaaggacggc gactcctacg tcgtctccgg ctccaaggcc
     3961 ttcatctcgg gcggcggcga gaacgaggtc tatgtgacca tggtccgtac cggtcaggag
     4021 ggccccaagg gcatttcgtg cctggtgatc gagaaggaca tgccgggagt gagcttcggg
     4081 gcgcaggaga ggaagctcgg ctggcactcg cagccgacgg cccaggtcaa tttcgacgac
     4141 gtgcgggtgc ccgcggagaa tctggtcggc ggcgagggcg agggcttccg gatcgcgatg
     4201 atgggcctcg acggcggccg gctcaacatc ggcgcctgct cattgggcgg ggcgcaacgc
     4261 tgcctcgacg aggcgatcgc ctacaccaag gaaaggaagc agttcgggac gccgatcgcc
     4321 gacttccagg cgacccagtt caccctcgcc gacatggaga ccgagctgca ggccgcccgg
     4381 atgctgcttt acgcggcggc ggtgaaggtg accgagaacg cgcccgacaa gtcgcgcttc
     4441 gccgccatgg ccaagcgcct cgccaccgac accggctcct cggtcgtcga ccgggcgctt
     4501 cagctccacg gcggctacgg ctacctccag gactatccgg tggagcgctt ctggcgcgac
     4561 ctgcgcgtcc attcgatcct cgagggcacc aaccagatca tgcgggtgat cgtcgcccgg
     4621 gacatgctga ggcagtgacg tccttcgata cgccgtttcg ccaagctcaa cggctagtca
     4681 ggatgagcgg ttgtgccttt ccgctcatcc tgagtagggg ctgagcttgg cgaagacccg
     4741 tatcgaagga ccggcacgag gcgcatgacc cccgacctca tcacccgcgt cgaaggcaag
     4801 gtcggccgcc tgcgcctcaa ccggcccaag gcgatccacg ccctcacgaa ggagatgtgc
     4861 agcgcgatga tcgccgcgct cctcccctgg cgcgaggacc cgtcggtgca tgcggtgatg
     4921 atcgaccatg ctgaaggccg cggcttctgc gccggcggcg acgtcgtcat gctggctcga
     4981 agcggcgccg gcgacggcgc ggaggcgcgc gccttcttcc acgaggaata tcggctcaac
     5041 cacctcctct tcacctacgc gaagccgacc gtcgccttca tggacgggat caccatgggc
     5101 ggcggcgtcg gaatctcgca gccgtgcaga tatcgcgtgg cgacggagaa tacccgcttt
     5161 gccatgccgg agacggcgat cggcctgttt ccggacgtcg gcggcggctg gtatctgcct
     5221 cggctgccgg ggcggacagg ccacttcctg gccctgaccg gcgcccgcct cgacggctcc
     5281 gaatgcctgc gcctcggatt ggccacccat tacatcccgt cgctcgcgct cgacgacctc
     5341 aaggccagga tgctggccga gccggagcgg ctcgaggcgc tgctcgagga gaacagcgcc
     5401 accccgccgc cggcgcggat cgaggcgaac ctgccgctga tcgaccgcct gttcgcctcg
     5461 gacgactatg aagacgtcct cgccgcgctg gaggccgacg gctccgactg ggcgaggacc
     5521 gagctcgaga cgctgcgcgg caagagccca caggcctgca aggtctcgct gcgactgctc
     5581 tacgacggtg cccgggtcca ggatttcgcc cacgagatga agcaggaata tgcggtggcg
     5641 acccgggtcg ttcagcgcca cgacttcgtc gagggcgtgc gggcgctgct ggtcgacaag
     5701 gacaacagcc cgcgctggga gccgtcgacg ccggagggcg tcacccagca cttgatcgac
     5761 acgatcttcg cgccgatgcc cgaaggcgag gaatggacgc cgctttagac cggcgaagcc
     5821 gtcattgcga gcgaagcgaa gcaatccagc ggctcggcgt ccgcaagctg gattgcttcg
     5881 tcgcttcgct cctcgcaatg acggggaaag cggaatccgg aggtaagatg aactacgaga
     5941 acatcctggt cgagcagcgc ggcgcggtca cgctcatcac cctcaatcgc cccaaggcgc
     6001 tcaacgcgct caacacggcc gtcctcgccg agctgatcgc cgccttcgcc gcttatgatt
     6061 cggacggcag ccagcgctgc gccgtgctca ccggaagcga gcgcgccttc gccgccggcg
     6121 ccgacatcaa ggagatgtcg gaccagggct tcgcctcgat gtattcctcc aacttcttcc
     6181 agggctggga gagggtcacg gccacccgca agccctggat cgccgccgtc gccggctacg
     6241 cgctcggcgg cggctgcgag gtggcgatga tggcggactt catcatcgcc gccgacaccg
     6301 cgcagttcgg ccagcccgag atcaaattgg gcgtcactcc gggaatgggc ggctcgcagc
     6361 gcctgacccg cgccgtcggc aaggccaagg cgatggagat gtgcctcacc ggccggatga
     6421 tgggcgccga ggaggccgag cgctccggcc tcgttgcccg ggtcgtgccc gcggcggagc
     6481 tgctcgacga ggcgatgaag gcggcggaag cgatcgcgtc catggccccg ctcgccgcga
     6541 tcgccaccaa ggagatggtc aacgccgcct tcgagaccgg gctggcgcag ggcatcgtct
     6601 tcgagcgccg cctgttccac ggcctgttcg gcacgcaaga ccagaaggaa ggcatggccg
     6661 ccttcgtcga caagcgtaag gcagagtgga aggggcgata gaaaacaagg ggcggaggga
     6721 ccccaacccg ttcgggctga gcttgtcgaa gccctccctt gatttctcgc ccgcccgaga
     6781 gaagggaagc cttcgacagg ctcagggcga acggtgtttc cggtgcccct tcttcggagt
     6841 tcgtaaatgg cacggatcgc attcatcggg ctcggcaaca tgggcggcgg gatggccgcg
     6901 aacctcgtca aggcggggca tgaggtgcgt gccttcgacc tgagcgaggc ggcgctggcc
     6961 aaggccgaat cgaacggctg cgcccgggcc ggctccgccg ccgaagccgt cgccgacgcg
     7021 gaggcggtcg tgaccatgct ccccgccgga aagcacgtcc gcgacgtcta cgaattgagc
     7081 gtgatcggca cggcgccggc cggcgccatc ctgatcgact gctcgaccat cgacgtcgcc
     7141 accgcccgcg aggagatcgc gaaagccgcg gccgcgggct atgcgatggt cgacgcgccg
     7201 gtctcgggcg ggatcgccgc tgccgaggcc ggcaccctca ccttcatggt cggaggcagc
     7261 gacgaggggt tcgagcgggc gcgcgagatc ctcgagaaga tgggcaaggc ggtgatccac
     7321 gccggcgggc cgggttccgg acaagcggcg aagatctgca acaacatgct gctcggcgcc
     7381 tcgatggtgg cgacctgcga gacgttcgtc cttgccgaga agctcggcct cgacccgcag
     7441 accttcttcg acatcgcctc caaggcctcg ggccagtgct ggtcgatgac cacctattgc
     7501 ccggttccgg gagtgggccc ggtcacgccc gccgaccgcg actatgaagg cggcttcgct
     7561 tcggcgctga tgctgaagga cctgaggctg gcgatggagg ccgcgcagag cgaggacgcc
     7621 tacacgccga tgggcgctca cgcggaggag ctctacaccc gcttcgccga ggcgctgggc
     7681 ggcggccgca aggatttttc ggggatcatc aagatgatcg acgacagctg ggaggcgtct
     7741 tgactcgtca ttccggcgaa agccggaatc catgaacacc gacgcctgag gctacactcc
     7801 tcggttcgtg tccatggatg ccggatcgag tccggcatga cgaacctagg acgagcggcg
     7861 gtagctggct cctgccctcc gcctctcgcc gccgccgtag cgggcggcga gctcggccgg
     7921 gccgggctcg gccaccggca gcttgacgat cgccgagcgg cgccgaagat cgtcgacgca
     7981 gcgcgccgcg gccttgccgt cccacaattc ggggcagcgc agagcgcgcg gtccggtcag
     8041 cgcccgttcc agctcgatca gcagggtctc gggagtcacc agccggttcg tcccttcggt
     8101 gatggtcacc ggccgctcgg tattctcgcg aagagtcagg caggggattc cgagataggt
     8161 ggtctcctcc tggattccgc ccgaatcggt gatcgccgcc gccgcgcccg tcaccaggct
     8221 catgaagcgc acatagggca agggctcgat cagacggacg cccgcgcgcg ccagccggcc
     8281 gtcgaacaag gccccggcca gccgcctggc cgtccgcgga tggaccggga agacgagggg
     8341 aagccgccgc tgggtctcca ccagggcgtc gaccaggcgg atgagctgcg ccggatcgtc
     8401 gacgttggag ggccggtgca gggtgacgac gccgtagccg ccgcgctcga gcccgagctc
     8461 gcccggcacg tccgccgcct cgatcgcggg gcggaccagc tcgaagctgt ccagcatgat
     8521 gttgccgatc cgggtgatcc ggccggtcgg gatgccctcg gcgaccaggt tctcgtcggc
     8581 gtcgggcgaa ggcgtccaca gcacgtcggc gaggacgtcg gtgacgaggc ggttgagctc
     8641 ctcgggcatg gcccggtcgc ggctcctgag ccccgcctcg agatggatga cggggatccc
     8701 gagcttggct ccggccatcg cgcaggcggc ggtcgagttg acgtcgccga cgacgaccag
     8761 ccagtccggc cggtcggcca ctgcaacctg ctcataggcg atcatcaccc ggccggtctg
     8821 cgcggcatgg ctgcccgagc cgatgccgag atgatggtcc ggctccggca gcctcaggtc
     8881 ggcgaagaag gcgtccgaca tgttcacgtc gtaatgctgg ccggtgtgga tcagcaccgg
     8941 ctcgaagccg ggcgcggccg caagggcgtg ccagagcggc gcgaccttca tgaaattcgg
     9001 tcgcgcggcg gcgatcagat ggacgcgctg ggccatggcg ccggaagtag cgcgaaagcc
     9061 gttaagatag ggttggagcc cgctgcccga tgacgttggg ctcgcagcgg acgagcgacc
     9121 gcccccacgc ccggatccgc tcgcgccgca cctcggcggc catgttcggc tcgaaccggt
     9181 cgacctcgcc ccgcatcgcg gaggcctcct ccagcgagga gaacagcccg caacccaggc
     9241 cggccagcat cgccgctccc agcgccgtgg tctcgacgaa gcgcggccgc tcgaccggga
     9301 gctcgaggat gtcggcgaga tcctgcgcca gccagtcgtt cgacgccatc ccgccgtcga
     9361 tgttgagcac gtcccacggt gctccgtcct cggcgaatcc tcgcatcagc gaatgggtct
     9421 ggtgcgccat cgcctcgagc gcggcccggg cgatatgcgc ccgcttcgtg gcgaaggtca
     9481 gtccgaagat cgcgccccgc gcattgggca gccagaaggg cgctcccagg ccgacgaacg
     9541 cgggcatgat cgtcactccc ccgctgtccg gcacggagcg ggcgagcgcc tcggtctcgg
     9601 cggcgctggc gagcagcccg aggtcgtcgc gaagccattt gacgaggctt ccggccacga
     9661 agatcgagcc ttcgagcgcg taggtccgct gcccgccgat ctgcgcggcg atggtggaca
     9721 gcagccgcca ctggctgacc cggagttcgt cgccggtatt ggcgaggatg aaggcgccgg
     9781 ttccgaaggt cgccttggtg ctgccgtacc acaggcaggc ctggccgatc gaggcggcct
     9841 gctggtcccc ggccagcccg gcgatcggga tgggcagccc gaaatgttcc gggagggtcg
     9901 ttccgaacga gccggcgcaa tcgacgatct cgggcagcat cgaccgggag acgccccaca
     9961 ggtcgagcag ccagtcgtcc cagcgtccct cgccgatgtg catcagcgcg gtgcgagagg
    10021 cgttggtcgc gtcgctgacg tggaggccgc cggtgagctt gaagaccagc cagctctcga
    10081 cggtgccgac gcacaaatcg tcgccggcct cgcgaagctg cggccaattc tccagcgccc
    10141 aggcgatctt ggatccgctg aaatagggat cgagcagaag cccggtcttc tcgcgaagct
    10201 cgccctcgtg atgcctgtcc ttcagcgtct ggcacagctc ggccgtgcgc cggtcctgcc
    10261 agacgatcgc gggggccagc gccgcgcccg tccggcggct ccagaagacg atcgtctcgc
    10321 gctggttggt gatccccagc gccgccaccc ggccggcgcc gccccccgct gcgaccacgg
    10381 cgcgggcgca ggcgaggctc gaggcccaga tctcctcggc gtcatgctcg acccagcccg
    10441 gctgcggata ggattggaca agctcgcgct gctcggtcgc gaggcacgac ccgtcggcgg
    10501 cgaacagcat cgcccgggtg ctggtcgtgc cttcgtcgat gacgagaatg aagtcgccgg
    10561 aaactggcct atccgcttgc atcgcctcgc tcatcctgag tagccggtga gcctgacgaa
    10621 ccggcgtatc gaaggacgcg cagccggtcc ttcgatacgg gccttcgcca ggctcagtcc
    10681 ctactcagga tgagcgagga ggaatgggca agcagaattt ctccatcgac cttttcgacc
    10741 tgctcgtcgt gggcggcggc atcaacggcg cggcgatcgc ccgcgacgcg gcgatgcgcg
    10801 gggcgagcgt gctcctggtc gagaaggacg acctcgccgc ccacacctcg tccgcctcgt
    10861 ccaagctcat tcacggcggc cttcgctatc tggagaatta cgagttccgc ctcgtccgcg
    10921 aggcgctggc cgagcgcgag atattgctga ggacggcgcc gcacatcgtc cgcccgctgc
    10981 gcttcgtcct tccccacggc cccggcttga ggccgcattg gatggtccgc tcgggccttc
    11041 tcctctacga tctcttctcg atccggggca gcctgccgcg ctcgcggcgg gtgggccggc
    11101 gcgagccgga gctccacgcg cctctcaagg agcggcggcg agtactcgcc tattgggacg
    11161 cctgggtcga cgacagccgg ctcgtcgtcc tcaacgccgt cgccgccgcc gagcacggcg
    11221 ccgagatcgc cgtcggcacc gagctcctct ccgcccgccg ctcggacgga gcctggcgcg
    11281 ccgagctgtc gggcggccgc accgtcgcgg cgaaggcgat cgtcaacgcg gccgggccct
    11341 gggtcgacga ggtcctctcg ggccgcctcg ccgagaccag cgccagccgg gtccggctgg
    11401 tcaagggcag ccacatcctg ggtcccgccc tctggcaggg agaccatggc tacatcctcc
    11461 agcaggcgga cggacgggtc gtcttcgcgc tccccttcaa gggcgacttc accctcatcg
    11521 gcaccaccga cgtcccggtc gggcggccgg aagacgccgc ggccgggccg gacgagatcc
    11581 gctacctgtg cgaggcggcc gaccgctatt tcacgaggca gatcgtcccc gcggacgtcg
    11641 tgtggagcta ttccggcatc cgcgccctct acgacgacgg cgccgccgac gcgaagtcgg
    11701 tgacccgcga ctatcatctg gagctcgacc cggatccggg cccgaagctc ctctccgtct
    11761 tcggcggcaa gctcaccacc gcccgggcgc tcgccgagga agcgctggac cgcctcggga
    11821 tcgagggccg ccgctcgacc tcgcacagct tcctgcccgg cggcgacctc tctcccggct
    11881 tcctcgaatg gctggacggt ctcgcgaagt ggatgcccca gcccctcctc gcccgactct
    11941 ccggcgccta cggaacccgt ctcagggaga tgatcggcgg cgcccgaagc ctcgcggacc
    12001 tgggccggcc tttcggcgcg ggcctccacg aggtcgagct gcgctggctc agggaccggg
    12061 aattcgcccg gaccgccgac gacgtgctgt ggcgccgcac caaactcggg ctggccatga
    12121 gccaggaaga ggctcgggcg gtggaggcct ggatgagaac cccgtcactg cgagcgtagc
    12181 gaagccgcca cgctccccgc agcgacggct agggcccctc gtgcagcgga tcggtgcccg
    12241 gtggcggcac ctcgtccagc gcctcgcgat tcttctgcgc ctcttgctcg atcgactcgt
    12301 cgtccgacgg aaggtcctct tcgtcgccgc ccatgtcctg ctctccttcg ccgcgctcaa
    12361 cgccccaccc gggaacccgc tccgcgcctt gcgctttgaa ccggccatgg caccctcggc
    12421 ctccgaagcc gccgccgccg ccgccggcaa gggcggcagc gtgatcggct ggaaactcga
    12481 ccgcgagcag cgcaaggagc tgctccagca gttcccgccc cgctacccca aggtcgacgc
    12541 cgaccacgtc accctgcagg ccggggcggg tgccgattcc gccctccccg acgagacgct
    12601 gggcgagatc gtcggccgcg ccgatgacgg ccgcggcgtc gaggcgatgg tggtcgccat
    12661 cggcggaacg accgagcggc ccgacggaag cgtctaccac atcacctggt cgctcgagcc
    12721 cgg
//
LOCUS       DASVQZ010000342         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_841747, whole genome shotgun sequence.
ACCESSION   DASVQZ010000342 DASVQZ010000000
VERSION     DASVQZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841747"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF547_09780"
     CDS             complement(<1..486)
                     /locus_tag="VF547_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX8263149.1"
                     /translation="MNKIFFAGAAVAALAAAPALAQPGGEGRARFAQPQTRAAVQAQV
                     QARFARADSDRDGFVTREEVRAAAEARREQRLERRAERREQLFERLDADHDGSISRAE
                     FDAPRAVAGGERSARRAMRAERRGQRFAHRGPGRGGAMMARFGARAFAAMDLDRDGRV
                     AF"
     gene            complement(535..1752)
                     /locus_tag="VF547_09785"
     CDS             complement(535..1752)
                     /locus_tag="VF547_09785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX8263150.1"
                     /translation="MVHSDSLSGGGAASPERLWRLAWAFVLILAALLFAQDIFATHFT
                     PWPSIRGQAIWGRDFANVWTSGRLALEGRLDLLYDLKAYSAYQQDLFDGALRAHNYSY
                     PPPTLLYTWAFGLLPYPVALLSWLALTGAAFAAAARPYLRDAGLPAWLAVIAPATLLN
                     VWAGHYGLLIGALWLGAFHLLPRRPGLAGVLIGLMLVKPHLAVLAPLILARRGEWRAF
                     AAAAATAAALVAASSLAFGPELWRTWLGVTVGVQTAMVDDVGTYFLTMMPTIVPTVSA
                     FGFPGFAAWTVQILAAAAAVGALLVHMPRDSRSAGLAGGVATFLVLPYAFNYDMTVPG
                     LAALLMLARLRREPESGPAFLLLLAFLLPLVVQTFGLLHLPVPALVLAALLALMLRGE
                     RALALKPGTAAAG"
     gene            2013..3605
                     /locus_tag="VF547_09790"
     CDS             2013..3605
                     /locus_tag="VF547_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263151.1"
                     /translation="MTSTPAERESASARQPRAWWEHPALVAAAVLACAVPLLWPDIPP
                     LVDLPGHMGRYHVQMEIGRTPVLAAFYEFKWALIGNLGVDLLVVPLAALIGLEPAVKL
                     ITLAIPVLTAAGFLWVAREVHGRIPPTAYFALPLTYSFPFVFGFVNFALSMALAFLAF
                     ALWLRLARLGHLKLRAGLFVPISVLLWVCHAFGWGTLGVMAFSAELVRQVDLKRPIWK
                     AGLNTGIHCLALAPPMLLMLLWRSGAVGGETAVWFNWRLKKNWVLMALRDRWEYWDYA
                     ALTVIGLVLVVGLLWWKRLEYSRNLAASAIFLAIVYVCLPRIVFGSNYADMRLAPFVI
                     ATAVIAIRYRPAWAGRAGAVFAAAGLLFAGARLAGNTYSFWMYDRAYDRELAAVDHIP
                     RGARVISFVGVKCRKDWAQTRLEHLPALALVRRDAFSNDQWSMSGAQLLTTIYPDPDS
                     WFSRDPSQQVLARPCRGERWLTLDQSLILFPRDRFDYVWLISPPRPDPALTRGLQPVW
                     QSGASVLYRVVDRRPIVLQEPQ"
     gene            3602..>4349
                     /locus_tag="VF547_09795"
     CDS             3602..>4349
                     /locus_tag="VF547_09795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8263152.1"
                     /translation="MTLPSLSIVVPCYNEEACLAELLGRLTAAARGAVGGDYEIVLVN
                     DGSRDRSWEMMRALSAQDPRLVAINLSRNHGHQLALTAGLDLCSGTRILIIDADLQDP
                     PELLPAMLAEMDRQSADVVYAVRRARAGETAFKRGTAKLFYRLLSRLAEIEIPRDTGD
                     FRLMSRRALDALLSLPEQARFIRGMVAWVGFRQVPIAYDRAERHAGQSKYPLSKMIRF
                     ALDAVTGFSTAPLRMASHIGLWLVAASGLLL"
BASE COUNT          623 a         1520 c         1569 g          637 t
ORIGIN      
        1 gaaggcgacc cggccgtccc ggtcgaggtc catcgccgcg aaggcgcggg cgccgaagcg
       61 ggccatcatc gcgccgccgc ggccagggcc gcgatgagcg aagcgctggc cccgcctctc
      121 ggcgcgcatc gcccggcgcg cgcttcgctc gccgccggcc acggcccggg gggcgtcgaa
      181 ctcggcgcgg ctgatcgatc cgtcatgatc ggcgtcgagc cgctcgaaga gctgctcgcg
      241 gcgctcggcg cggcgctcga gccgctgctc ccggcgggcc tcggcggcgg cgcggacctc
      301 ctcgcgggtg acgaagccgt cgcgatcgga atcggcgcgc gcgaagcggg cctggacctg
      361 ggcctggacc gccgcgcggg tctgcggctg ggcgaagcgg gcgcggcctt cgccgcccgg
      421 ctgggcgaga gcgggggccg cggcgagcgc agccacagcc gcgccggcga aaaatatctt
      481 gttcatttca actccttgga acgcggggga ggtctcccct tccacgcccg ccctctagcc
      541 ggcggcggct gtaccgggct ttaacgcgag cgcccgctcg ccgcgcagca tcagcgccag
      601 cagggcggcg aggaccagcg ccgggaccgg cagatggagc agcccgaagg tctggacgac
      661 gagcgggaga aggaaggcga ggaggaggag gaaggccggc ccgctttccg gctcgcggcg
      721 caggcgggcg agcatcagca gcgccgccag ccccggcacg gtcatgtcgt agttgaaggc
      781 ataggggagg acgaggaagg tcgcgactcc cccggccagg cccgccgagc ggctgtcgcg
      841 cggcatatgg acgagaaggg cgccgacggc ggcggcggcc gccaggatct gcaccgtcca
      901 ggccgcgaag cccgggaagc cgaaggcgga gacggtcggg acgatggtcg gcatcatcgt
      961 caggaaatag gtgccgacgt cgtcgaccat cgcggtctgg acgccgaccg tgacgccgag
     1021 ccaggtccgc cacaattcgg gcccgaaggc cagcgacgac gcggccacga gggcggccgc
     1081 ggtggccgcg gcggcggcaa aggcccgcca ctcgccgcgg cgggcgagga tcagcggcgc
     1141 gaggacggcg aggtgcggct tgaccagcat cagtccgatc aggacgccgg ccagccccgg
     1201 gcggcgcggg aggaggtgga aggcgccgag ccagagggcg ccgatcagca ggccgtaatg
     1261 gccggcccag acgttgagca gggtcgcggg cgcgatcacc gccagccagg ccggcaggcc
     1321 cgcgtcgcgc agataggggc gcgccgcggc ggcgaaggcg gcgccggtca gcgccagcca
     1381 ggaaagcagc gcgacgggat agggaagcag cccgaacgcc caggtgtaga gcagggtcgg
     1441 cggcggatag gaataattgt gggcgcgcag ggcgccgtcg aacagatcct gctggtaggc
     1501 gctgtaggcc ttgaggtcgt agagcaggtc gagccggcct tcgagagcga gccggcccga
     1561 ggtccagacg ttggcgaagt cgcgccccca gatggcctgg ccgcggatcg acggccaggg
     1621 cgtgaagtgg gtggcgaaga tatcctgggc gaaaagcaga gcggcgagga tcaggacgaa
     1681 ggcccaggcg agcctccaga gccgttccgg cgatgctgca ccgcccccgc tcaagctgtc
     1741 cgaatgaacc atccgcccgt ccatgcctct gggctaggtg aacagggtta acgaagggtt
     1801 atcctccccg ccgcgaagcg gtggggaggg ggaccatgcg cagcatggtg gaggggtgtt
     1861 tggcgtcgaa gccgggagtt tccgagagga tcgcgcacgt cgaggcaatt acccctccac
     1921 caccctgcgg gcggtccccc tccccatggc cgccgcgatg gggaggattt aagtgttgaa
     1981 tttcgccgcc ggagggggga ggcaagcgcg ccatgacctc cactcccgcc gaaagagaga
     2041 gcgccagcgc ccgccagcct cgcgcctggt gggagcatcc ggcgctggtc gccgcggcgg
     2101 tcctcgcctg cgccgtgccg ctgctctggc ccgacatccc gccgctggtc gacctgccgg
     2161 gccatatggg ccgctaccac gtccagatgg aaatcggccg gacgccggtg ctcgcggcct
     2221 tctacgagtt caagtgggcg ctgatcggca atctcggggt cgacctcctg gtcgtcccgc
     2281 tcgcggccct gatcgggctc gagccggcgg tgaagctgat caccctcgcc atcccggtgc
     2341 tgaccgcggc gggcttcctg tgggttgcgc gcgaggtcca tggccgcatc ccgccgaccg
     2401 cctatttcgc gctccccctg acctacagct tcccgttcgt cttcggcttc gtcaatttcg
     2461 ccttgtcgat ggcgctcgcg ttcctcgcct tcgccttgtg gcttcggctc gcccggctcg
     2521 ggcacctgaa gcttcgcgcc ggcctgttcg tcccgatctc ggtgctgctg tgggtgtgcc
     2581 acgccttcgg ctggggcacg ctcggagtga tggccttctc ggccgagctg gtgcgccagg
     2641 tcgacctgaa gcggccgatc tggaaggccg gcctgaacac cggcatccac tgcctcgcgc
     2701 tcgcgccgcc gatgctcctg atgctgctct ggcggagcgg cgcggtcggc ggcgagaccg
     2761 ccgtctggtt caactggagg ctcaagaaga actgggttct gatggccctc cgcgaccgct
     2821 gggaatattg ggattatgcg gcgctgacgg tgatcgggct cgtcctcgtc gtcggcctgc
     2881 tgtggtggaa gaggctcgaa tattcgcgca acctcgccgc ctccgcgatc ttcctcgcca
     2941 tagtctacgt ctgcctgccc aggatcgtgt tcggctccaa ttacgccgac atgcggctcg
     3001 ccccgttcgt gatcgcgacg gccgtgatcg cgatccgcta ccggccggcc tgggcgggcc
     3061 gggccggcgc cgtcttcgcg gccgccggcc ttctgttcgc cggcgccagg ctggcgggca
     3121 acacctacag cttctggatg tacgaccgcg cctacgaccg ggagctggcc gcggtcgacc
     3181 atatcccacg cggcgccagg gtcatttcgt tcgtcggcgt caagtgccgc aaggactggg
     3241 cccagacccg gctcgagcat ctccccgcct tggccctggt ccggcgcgac gccttctcca
     3301 acgaccagtg gtcgatgagc ggcgcccagc tcctcaccac catctatccc gatcccgaca
     3361 gctggttctc gcgcgacccc tcgcagcagg tcctcgcccg cccgtgccgc ggggagcgct
     3421 ggcttaccct cgaccagtcg ctgatcctct tcccgcggga ccggttcgac tatgtctggc
     3481 tgatcagccc tccccggccc gacccggcgc tgacccgggg gctgcagccg gtctggcaaa
     3541 gcggcgccag cgtgctgtat cgggtcgtcg accgccgccc gatcgtcctc caggaacccc
     3601 aatgaccttg ccgtccctct cgatcgtcgt cccctgctac aacgaggagg cgtgcctcgc
     3661 cgagctgctc ggacggctga ccgcggcggc gcgcggcgcg gtcggcgggg attacgagat
     3721 cgtcctggtc aacgacggct cgcgcgaccg cagctgggag atgatgcgcg ccctgtcggc
     3781 gcaggacccg cggctggtgg cgatcaacct gtcgcgcaac cacggccacc agctcgccct
     3841 gacggcgggg ctcgacctgt gctcggggac acgcatcctg atcatcgacg ccgacctcca
     3901 ggatccgccc gagctgcttc ccgccatgct ggcggagatg gaccggcaga gcgccgacgt
     3961 cgtctacgcg gtgcggcggg cgcgggccgg cgagaccgcg ttcaagcgcg gcaccgccaa
     4021 gctcttctac cggctgctgt cgaggctggc cgagatcgag atcccccgcg acaccggcga
     4081 tttccggctg atgagccggc gcgcgctcga tgccctgctg agcctgccgg agcaggctcg
     4141 gttcatccgc ggaatggtcg cctgggtcgg attccgccag gtgccgatcg cctacgaccg
     4201 cgccgagcgc catgccggcc agagcaaata tccgctgtcg aagatgatcc gcttcgcgct
     4261 cgacgcggtg acgggcttct cgaccgcgcc gctgcggatg gcgagccata tcggcctgtg
     4321 gctggtcgcg gcttcggggc tgctgctca
//
LOCUS       DASVQZ010000343         6705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_552791, whole genome shotgun sequence.
ACCESSION   DASVQZ010000343 DASVQZ010000000
VERSION     DASVQZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552791"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VF547_09800"
     CDS             <1..1524
                     /locus_tag="VF547_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX8263153.1"
                     /translation="GVLNQANDVTKTVTVERRLSFQVQLADRLRGLYDPEEVKQAAAE
                     WLGRYLGAARVGFAELDEEKDVATVRQDWTRDPSVRSLAGQHTSIAGFGDEALAFFRA
                     GQPLAIPDIRQLALDSADRTAAWEAAGVRALITLPLVRDGRLKALLYVHEPQPRAWRR
                     SDVAIVRDVAERSWAAVERARAEQSLRESEDHYRHAVELNPQVAWTSLPDGRLNRVAK
                     RWFEWTGTTGLEGSWKEGMHPDDHSKTLEEWDRCLASGEPYDVEHRVRRRDGRYRWAR
                     SRAFPRRDAEGNIILWYGNTEDIHERKVAEEHQRLLINELNHRVKNTLASVQAIAFQT
                     LRGDVPLAEARARFEARLMALSAAHNLLTEESWVGASLERVVKDATEHLAGDSSRFDV
                     EGEPLRLAPRAALALAMALHELGTNAAKYGALSVEDGRVSIRWALDGDRLRFEWRESG
                     GPTVVAPARRGFGSRLIERGLEADLGGAAALHFESGGLRCEIAASLAAIRAPERTFD"
     gene            1517..1870
                     /locus_tag="VF547_09805"
     CDS             1517..1870
                     /locus_tag="VF547_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8263154.1"
                     /translation="MTERRLRILVVEDEMLVAMNIEDMLLDLGHEVAGLASRLGPALA
                     LARESRFDAAMLDVNLAGEPSFPIADLLIERGIPFLFATGYGRQGVDERYRGHPMLQK
                     PFRSAELGAALAALA"
     gene            complement(1880..2587)
                     /locus_tag="VF547_09810"
     CDS             complement(1880..2587)
                     /locus_tag="VF547_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX8263155.1"
                     /translation="MAAHNSRVSGNGNSVTQAISSRRSVRAFLPRPVERSTIEEMLAL
                     AARAPSGGNLQPWHVDVLAGAPLEALKAEVRARRAAEPAGEGLEFTVYPSPLPEPFRS
                     RRFAGGEALYAWLGIAREDKAGRLAQFERNYDFFGAPAALFFSIDRLFDRPQWAHLGM
                     FMQNLMLLAEERGLATCPQEAWAAVHRTVAAHLGLPAERILYCGMALGYADESAPINR
                     WRTERAPVEDFATFRGF"
     gene            2586..3827
                     /locus_tag="VF547_09815"
     CDS             2586..3827
                     /locus_tag="VF547_09815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8263156.1"
                     /translation="MPLLDKMLRKLLTRGELTIIGPDGKSTHFGAPDPELKPVTVRLM
                     DRKVAMQIARDPTLGTVETWMDDRLRLEQGEIIDLVLLIRRNRRWEERTRPNEFLKKT
                     GRLRHLASTLNWKSRSLKNVVHHYDIGNDLYRLFLDPDMQYSCAYFTDPGNSIEQAQL
                     DKKAHIASKMLLKPGQKVLDIGCGWGGLALYLNRVADVDVLGVTLSKEQLAVARQRAE
                     EAGVSDRVRFELCDYRDVTGQFDRIVSIGMFEHLGTAYFPVFFRKVRELLHPDGAALV
                     HTMGRMGKPGTTDKFMQKYIFPGGYLPALSEIVSASERERLIMADCEALRLHYVYTLR
                     AWYERFKAKHDEVVAMYDERFYRLYLFYLAGSMTMFTDGAMVVYQLQYLRSRYAAPIT
                     RNYMIEDEERLRKAGGPPPQR"
     gene            3824..5071
                     /locus_tag="VF547_09820"
     CDS             3824..5071
                     /locus_tag="VF547_09820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8263157.1"
                     /translation="MNWLLDKALKSYVKSGEIILTDHDGTIHAYGSPDGPFGSVSVRF
                     ADAGVARDILRDPGLGAAEAYMDGRLVVERGEILDLVNIVRGSHRWEDSQGPNPFLRK
                     GGRLGHWLRTLNWRPSALKNVSHHYDVGNDFYRLFLDEDLQYSCGYFTDPANSVEQAQ
                     LDKKTHVAAKLRLRPGMKVLDIGCGWGGLALYLNRVADVDVLGITLSKAQLDLARQRA
                     VQAGVSDRVRFELIDYRDVTGEFDRVASVGMMEHVGKPFYRAYFRKIRDVLKPDGIAV
                     VHTMARLGGPGTTDRFMQKHVFPGGYLPALSEIVGASEQERLIMADCETWRLHYVHTL
                     KAWHDRLKSNEAEAVRLQGERIYRLYLFYLAASLTMFRDAPMAVYQIQYLRDRGATPI
                     TRDYMVDEERALRERAGAAARGG"
     gene            5142..6407
                     /locus_tag="VF547_09825"
     CDS             5142..6407
                     /locus_tag="VF547_09825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8263158.1"
                     /translation="MWLLDKMLRKVVQKGDLTVIDHDGKEYHYGDGGPAPHGPVRVRF
                     TDKGAANHIIKDPRVGAGEVFMDGRMVIEQGDLRDLVLLIRYNAPVERQGDLKPRGPV
                     RKAIGRIAGRLDQINWKTRSRHNAEHTYNLTRRLFELFLDEDRQYTMAYYRDLSNSLE
                     KAQLDKKAHIASKMYLKPGMKVLDIGCGWGGFALYLHRMYGVEVLGVALAPDQIEFSN
                     ERAREAGVSDKVKFELMDYRDIQGQFDRISNVGLIEHLGTPHYPGFFEHMRRLLKDDG
                     VMVSHCCGRMGEPGVTDKWTRKYIFPGGYIPALSELVTQAEKHRLIVTDVEAMRYHYA
                     PTLQEWYNRTVMNRDEIISLYDERFYRMWLFYLAGAEAAFRYGGMVNWQLQYVKRRDA
                     IPMTRDYMYEESARLREMEEPPQWHLAAE"
BASE COUNT         1135 a         2307 c         2242 g         1021 t
ORIGIN      
        1 ggcgtcctca accaggcgaa cgacgtgacc aagacggtga cggtcgagcg gcggctgtcg
       61 ttccaggtcc agctcgccga ccggctgcgc ggcctctacg atcccgagga ggtcaagcag
      121 gccgcggccg aatggctcgg ccgctacctc ggcgcggccc gggtcggctt tgccgagctc
      181 gacgaagaga aggacgtcgc gacggtccgt caggactgga cccgggatcc ctcggtccgg
      241 agcctcgccg gccagcacac cagcatcgcc ggcttcggcg acgaggccct ggccttcttc
      301 cgcgccggcc agccgctcgc catccccgac atccgccagc tcgcgctcga ctcggccgac
      361 cggaccgccg cctgggaagc ggctggagtg cgggcgctga tcaccctccc gctggtccgc
      421 gacggcaggc tcaaggcgct gctctacgtg cacgagccgc agccgcgcgc ctggagacgg
      481 tccgacgtgg cgatcgtgcg tgacgtcgcc gagcgcagct gggcggcggt tgagcgggcg
      541 cgggccgagc agtcgctgag ggagagcgag gaccattacc ggcacgccgt cgagctcaac
      601 ccgcaggtcg cctggacctc gctgcccgac ggccgcctca accgggtcgc gaagcgctgg
      661 ttcgagtgga ccggaacgac cggactcgag gggagctgga aggagggcat gcacccggac
      721 gatcactcca agaccctcga ggagtgggac cggtgcctgg cctccggcga gccctacgac
      781 gtcgagcatc gggtgaggcg ccgcgacggc cgctatcggt gggcgcgatc gcgggccttc
      841 ccgcgccgcg atgccgaggg aaacatcatc ctgtggtacg gcaacaccga ggacatccat
      901 gagcgcaagg tcgccgagga gcatcagcgg ctgctgatca acgagctcaa ccaccgggtg
      961 aagaacacgc ttgccagcgt ccaggcgatc gccttccaga cgctgcgcgg ggacgtgccg
     1021 ctcgccgagg ctcgggcgcg gttcgaggcg cggctgatgg cgctgtccgc ggcccacaac
     1081 ctgctcaccg aggagagctg ggtcggggcc tcgctcgagc gggtggtgaa ggacgccacc
     1141 gagcacctcg ccggcgactc ttcccgcttc gacgtcgaag gggagccgct ccggctcgcg
     1201 ccgcgggccg cgctcgcgct ggcgatggcg ctgcacgagc tcggcaccaa cgccgccaaa
     1261 tatggcgcgc tctcggtcga agacggccgg gtgtcgatcc gctgggcgct ggacggcgac
     1321 cggctgcgct tcgaatggcg cgagagcggc ggcccgacag tggttgcgcc ggcgcggcgc
     1381 gggttcggct cgcggctgat cgagcggggg ctggaagccg atctcggcgg cgccgccgcc
     1441 ctccatttcg aatcgggcgg cctgcgctgc gagatcgcgg cctcgctcgc cgcgatccgc
     1501 gccccggagc ggacgtttga ctgagcggcg cctgaggatc ctcgtcgtcg aggacgagat
     1561 gctggtggca atgaacatcg aggacatgct gctcgatctc ggccacgagg tggcgggcct
     1621 ggccagccgc ctcggccccg ccctcgcgct cgcccgcgag agccgcttcg acgcggcgat
     1681 gctcgacgtc aacctcgccg gcgagccgag ctttcccatc gccgacctgc tgatcgagcg
     1741 cggcatcccc ttcctgttcg ccaccggcta cggccgccag ggcgtggacg agcgctatcg
     1801 cggtcatccg atgctccaga agccgttccg gtcggccgag ttgggtgcgg cgctggcggc
     1861 gcttgcctga acctccccat cagaagccgc ggaaggtcgc gaagtcctcc accggcgccc
     1921 gctcggtccg ccagcggttg atcggcgccg actcgtccgc atagccgagc gccatgccgc
     1981 aatagaggat gcgctccgcc ggcaggccga gatgggccgc caccgtccga tgcaccgccg
     2041 cccatgcctc ctgcggacag gtggcgaggc cccgctcctc ggcgagcagc atcagattct
     2101 gcatgaacat gcccagatgc gcccattgcg gccggtcgaa cagccggtcg atcgagaaga
     2161 acagggcggc cggggcgccg aagaaatcat agttgcgctc gaactgcgcg agccgccccg
     2221 ccttgtcctc gcgcgcgatg ccgagccagg cgtagagcgc ctcgccgccg gcgaagcgcc
     2281 gcgaccggaa cggctccggc agcggcgagg gatagacggt gaattcgaga ccctcgcccg
     2341 ccggctccgc ggcacggcgg gcgcgcacct ccgccttcag cgcctcgagc ggcgcgcccg
     2401 cgagcacgtc gacgtgccag ggctggaggt tgccgccgga gggcgcccga gcggcgagcg
     2461 ccagcatctc ctcgatcgtc gaccgctcga ccggccgcgg caggaatgcg cgcaccgatc
     2521 ggcgcgacga aatcgcctgc gtaacggaat tgccattgcc ggacacgcgg ctattgtgcg
     2581 cggccatgcc gcttctagac aagatgctcc gcaagctcct cacgcgaggt gagctcacca
     2641 tcatcggccc cgacggaaag tcgacgcatt tcggtgcgcc ggacccggag ctgaagccgg
     2701 tcaccgtccg gctcatggac cgcaaggtcg ccatgcagat cgcgcgcgat ccgacgctcg
     2761 gcacggtgga gacctggatg gacgaccggc ttcggctcga gcagggcgag atcatcgacc
     2821 tcgtcctgct gatccgccgc aatcgccgct gggaggaaag gactcggccg aacgagttcc
     2881 tgaagaagac gggcaggctc cgccacctcg caagcacgct gaactggaag tcgcggtcgc
     2941 tgaagaacgt cgtccaccat tacgacatcg gcaacgatct ctaccgcctc ttcctcgatc
     3001 ccgacatgca atatagctgc gcctatttca ccgatcccgg caacagcatc gagcaggccc
     3061 agctcgacaa gaaggcccat atcgcctcga agatgctgct caagccgggg cagaaggtgc
     3121 tcgacatagg ctgcggctgg ggcgggctcg ccctctacct gaaccgggtc gccgacgtcg
     3181 acgtgctcgg ggtgaccctc tccaaagagc agctcgccgt cgcccgccag cgcgcggagg
     3241 aggccggggt ctccgaccgg gtgaggttcg agctgtgcga ctatcgcgac gtcaccggcc
     3301 agttcgaccg gatcgtctcg atcggcatgt tcgagcatct cggcaccgct tatttccccg
     3361 tcttcttccg caaggtgcgc gagcttctcc atcccgacgg cgcggccctc gtccacacga
     3421 tgggacggat gggcaagccg ggcacgaccg acaagttcat gcagaaatac atcttccccg
     3481 gcggctatct gccggccctg tcggagatcg tctcggcgag cgagcgcgag cggctgatca
     3541 tggcggactg cgaggcgctt cgcctccact acgtctatac cttgcgggcc tggtacgagc
     3601 gcttcaaggc gaagcatgac gaggtcgtcg cgatgtacga cgagcgcttc taccggctct
     3661 atctcttcta tctcgccggc tcgatgacga tgttcaccga cggagcgatg gtcgtctacc
     3721 agctccagta cctgcgcagc cgctacgccg ccccgatcac ccgcaactac atgatcgagg
     3781 acgaggagcg gcttcggaag gccgggggac ccccgccgca aagatgaact ggctgctcga
     3841 caaggcgctc aaaagctacg tcaagagcgg cgagatcatc ctgaccgacc atgacggcac
     3901 catccatgcc tacggctcgc ccgacggccc gttcggctcg gtgtcggtcc gcttcgccga
     3961 tgccggcgtg gctcgcgaca tcctgcgcga tcccggcctc ggcgcggccg aagcctatat
     4021 ggacggccgg ctggtcgtcg agcgcggcga gatactcgac ctcgtcaaca tcgtccgcgg
     4081 aagccaccgc tgggaggatt ctcaaggtcc caatccgttc cttcgcaagg gcggccgcct
     4141 cggccactgg ctgcgcacgc tcaactggcg gccgagcgcg ctgaagaacg tctcccacca
     4201 ttacgacgtc ggcaacgatt tctaccggct cttcctggac gaggacctgc aatacagctg
     4261 cggctatttc accgatccgg cgaacagcgt cgagcaggcg cagctcgaca agaagaccca
     4321 cgtcgcggcc aagcttcggc tgcggccggg aatgaaggtg ctcgacatag ggtgcggctg
     4381 gggcggcctg gccctctatc tcaaccgggt cgccgacgtc gacgtgctcg gaatcaccct
     4441 gtcgaaggcg cagctcgatc tggcccggca gcgggccgtc caggccggcg tctcggaccg
     4501 agtccgcttc gagctcatcg actatcgcga cgtcacgggg gaattcgacc gggtcgcgtc
     4561 ggtgggcatg atggagcatg tcggcaagcc cttctaccgc gcctatttcc gcaagatccg
     4621 ggacgttctg aagcccgacg ggatcgccgt cgtccacact atggcgcgcc tcggcgggcc
     4681 ggggacgacc gacaggttca tgcagaagca cgtcttcccg ggcggatatc tgccggcctt
     4741 gtcggagatc gtcggagcga gcgagcagga acggctcatc atggccgact gcgaaacctg
     4801 gcggcttcac tacgtccata ccctgaaggc ctggcacgac cggctcaagt cgaacgaggc
     4861 cgaggcggtg cgccttcagg gcgagcgaat ctaccgcctc tatctcttct acctggcggc
     4921 ctcgctgacc atgttccgcg acgcgccgat ggccgtctac cagatccagt atctgaggga
     4981 ccggggggcg acgccgatca cgcgggacta tatggtggat gaggagaggg cgctgaggga
     5041 gcgggcgggg gccgccgcgc gcggcggctg acggcttccg aagcttgacc caagcgcctc
     5101 ctccatgctc attgcgccca aaatccaggg gtgtctgacc gatgtggctg ctcgacaaaa
     5161 tgcttcgcaa ggtcgtccag aagggcgacc tcaccgtcat cgaccatgac ggcaaggaat
     5221 atcattacgg cgacggcggc ccggcgccgc acggcccggt ccgggtccga ttcaccgaca
     5281 agggcgccgc caaccacatc atcaaggatc cgcgggtcgg cgccggcgag gtcttcatgg
     5341 acggccggat ggtcatcgag cagggcgacc tgcgcgacct cgtcctcctc atccgctaca
     5401 acgcgccggt cgagcggcag ggcgacctca agccgagggg tccggtccgc aaggcgatcg
     5461 gccggatcgc cggacggctc gaccagatca actggaagac ccgctcgagg cacaatgccg
     5521 agcataccta caacctcacc cggcgcctgt tcgagctgtt cctcgacgag gaccggcagt
     5581 acacgatggc ctattatcgc gacctgtcga acagcctcga gaaggctcag ctcgacaaga
     5641 aggcgcacat cgcctcgaag atgtacctga agccgggcat gaaggtgctc gacatcggct
     5701 gcggctgggg cggcttcgcg ctctacctcc accgcatgta cggcgtcgag gtgctcggag
     5761 tcgccctcgc gcccgaccag atcgaattct cgaacgagcg ggcgcgcgag gcgggggtct
     5821 cggacaaggt caagttcgag ctgatggact atcgcgacat ccagggccag ttcgaccgga
     5881 tcagcaacgt cggcctgatc gagcatctcg ggacgccgca ttatcccggc ttcttcgagc
     5941 atatgcgccg gctgctgaag gacgacggcg tgatggtctc ccattgctgc gggcggatgg
     6001 gcgagccggg cgtgaccgac aagtggacgc gcaagtacat cttcccgggc ggctatatcc
     6061 cggcgctgtc ggagctggtc acccaggccg agaagcaccg gctgatcgtc accgacgtcg
     6121 aggcgatgcg ctaccattac gcgccgacgc tgcaggaatg gtacaaccgc accgtcatga
     6181 acagggacga gatcatctcg ctctacgacg agcgcttcta ccggatgtgg ctcttctacc
     6241 ttgccggggc cgaggcggcc ttccgctacg gcgggatggt caactggcag ctccagtatg
     6301 tgaagcgccg cgacgccatc ccgatgaccc gcgactatat gtacgaggaa tcggcccgcc
     6361 ttcgcgagat ggaggagccg ccgcaatggc atctggcggc cgagtgatcc gctcctaagt
     6421 cctcccctgc attcgcaggg gagggggacc acccgaaggg tggtggaggg gctcaacgga
     6481 gtctgaagaa cccctccaca ggcttcgcct ggtccccctc cccgcgaaat cgcggggagg
     6541 attttagtct tcccgatcct cccgaggctc ctcgcggcgc tccatccgtg ccgcgcgctc
     6601 cgcctggcgc tcgtccaggc tgatcgtgcc gtcgcggttc gaatcgatcc ggtcgaagcg
     6661 ctggagcgcc gcccgctgcg cctcggcgaa ggcgacccgg ccgtc
//
LOCUS       DASVQZ010000344         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_650647, whole genome shotgun sequence.
ACCESSION   DASVQZ010000344 DASVQZ010000000
VERSION     DASVQZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650647"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1068
                     /locus_tag="VF547_09830"
     CDS             346..1068
                     /locus_tag="VF547_09830"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8263159.1"
                     /translation="MLGLYHLIALAFFSAFVVAELMLPGRAYPLLQSWHARAAASTLA
                     YFAVATFAPLLWDGTLGAYRLFDATALPFWAQLLGGFVALELGIYLWHRTLHAVDPLW
                     RWFHQMHHSAERVDVWGAFYFSPLDMLGFAFLGSLVLVLGFGLSGEAALAVNLLVTFL
                     GLFQHANIRTPRWLGYLVVRPEGHAAHHERGVHAFNYCDLPLWDMLFGTFRNPSEFEG
                     EAGFWDGASGRIGPMLIGRDVA"
     gene            complement(1195..3111)
                     /locus_tag="VF547_09835"
     CDS             complement(1195..3111)
                     /locus_tag="VF547_09835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8263160.1"
                     /translation="MDRSVKPGDSFWHFVNGNWDKTTQIAADRTSAGVGVLLADEAER
                     QVRAIVEDLARDPAKSGKIGQQVGDYYASWMDEAGIEAAGTAPLKPYLARFASINNRT
                     DLIRAFAEPGVMSPIGVGILPDPADPTRYIAAAGQGGLGLPNRDYYLLEGEKYDLIRK
                     AYRAYVVKMQQLAGIADAEAKADRIIALETALAKVHWEPARQRDIKQIYNPMNRQQLA
                     ELAPQLEWPVFLEAAGLGSVPTVIAAEKSAITETGKMLETVPLSTWKDYLAFHFVSSH
                     AAYLPKAFDQASFDFYSKTLRGVPQQRERWKRGIQMINGSLGEAVGRIYVERHYPAES
                     NRQMAELIANLRSGLEARLAASTWMDAATKEQARAKLAAFDPRIGHPTKWIDYSSLQV
                     KRGDLLGNVVRTQKFGHELQLSRLGKPVDRALWDMNPQEVNAYYNPLMNQITFPAAIL
                     QPPYFDPKADPAINYGSIGAIIGHEIGHGFDDQGRQFDATGRIRDWWSASSGTEFTKR
                     AEKLGKQFDAYEPIPGTKINGKLTMGENIGDLGGLEMAYAAYRRYVAQHGEPKVINGL
                     TGDQRFFIAYGQSWRSKAREGALRERLLTDPHSPPEFRVNGIVRNVDAWYKAFNVKPG
                     DKMYLPPEDRVSIW"
BASE COUNT          439 a         1031 c         1054 g          588 t
ORIGIN      
        1 aagggcggcg gcgcccgacg aagcgatgaa ggtgcggcga tccaagggta acctcctgct
       61 tgagacggcg acgctttagg cgcgcgcgag cggccggcgc aacgttccgt catcccggcg
      121 aaggccggga tctcggggag acccgccgag acttcttgtc ccgaggcccc ggctttcgcc
      181 ggggtgacgg gatgtaacaa tagacaccat aaaccgcgcc ccgcggcatc gacccgccac
      241 ccgccgcctc cattacccaa atcacaggtt ccggccggca gcgtttcgtc ccctcacgtg
      301 ccggccgggc ctccccttcc ccgcttttcg aaatggagga ccttggtgct cggtctctac
      361 catctcatcg ctctggcctt cttctcggcc ttcgtcgtcg ccgagctcat gctgcccggc
      421 cgcgcctatc cgctgctgca gtcgtggcat gcgcgcgcgg cagcctcgac cctcgcctat
      481 ttcgcggtcg cgaccttcgc tccgctgctt tgggacggga cgctcggcgc ctaccggctg
      541 ttcgacgcca ccgccctccc cttctgggcg cagctgctcg gcggcttcgt cgctctggag
      601 ctcgggatct acctttggca ccggaccctc catgccgtgg acccgctgtg gcgctggttc
      661 caccagatgc accacagcgc ggagcgggtc gacgtctggg gcgccttcta tttctcgccg
      721 ctcgacatgc tcggcttcgc cttcctcgga agcctggttc tggtgctcgg cttcggcctc
      781 agcggcgagg ctgcgctcgc ggtcaacctc ctcgtgacct tcctcggcct gttccagcac
      841 gccaatatcc gcactccgcg ctggctcggc tatctggtcg tccgtcccga gggccatgcg
      901 gcccatcacg agcgcggggt ccacgccttc aactattgcg acctgccgct ctgggacatg
      961 ctgttcggca cgttcaggaa cccaagcgag ttcgagggcg aagccggctt ctgggacggc
     1021 gcctcggggc ggatcgggcc gatgctgatc gggcgggacg tggcgtagga agcgatcgtc
     1081 atcccggcga aagccgggac ctcgggacaa gaagtctcgg cgggtctccc cgagatcccg
     1141 gctttcgccg ggatgacggg gatgtgaaaa ggggcgccga agcgcccctt ccccttacca
     1201 gatgctgact cggtcttccg gcggcaggta catcttgtcg ccgggcttca cgttgaacgc
     1261 cttgtaccag gcgtcgacgt tgcggacgat cccgttgacc cggaattccg gcgggctgtg
     1321 cggatcggtc aggagccgct cgcgcagcgc gccctcgcgc gccttggagc gccagctctg
     1381 gccgtaggcg atgaagaagc gctggtcgcc ggtgaggccg ttgatcacct tgggctcgcc
     1441 atgctgggcg acgtagcggc gataggcggc ataggccatt tcgagcccgc cgagatcgcc
     1501 gatattctcg cccatggtga gcttgccgtt gatcttggtc cccgggatcg gctcataggc
     1561 gtcgaactgc ttgccgagct tctcggcccg cttggtgaac tcggtgcccg agctcgccga
     1621 ccaccagtcg cggatccggc cggtggcgtc gaactgacgg ccctgatcgt cgaagccgtg
     1681 gccgatctcg tggccgatga tcgctccgat cgagccgtag ttgatcgccg ggtcggcctt
     1741 cggatcgaaa taaggcggct ggaggatcgc ggccgggaag gtgatctggt tcatcagcgg
     1801 gttgtaatag gcgttcacct cctgcgggtt catgtcccac agcgcccggt cgaccggctt
     1861 tccgagcctg gagagctgaa gctcgtggcc gaacttctgc gtgcggacca cgttgccgag
     1921 gagatcgccg cgcttcacct ggaggctgga ataatcgatc cacttggtcg ggtggccgat
     1981 cctgggatcg aaggcggcga gcttggcgcg cgcctgctcc ttggtcgcgg cgtccatcca
     2041 ggtcgaggcg gcgaggcgcg cctcgaggcc ggagcggagg ttggcgatca gctcggccat
     2101 ctggcggttg ctctccgccg gatagtggcg ctcgacgtag atccggccga ccgcctcgcc
     2161 gagcgagccg ttgatcatct ggatcccgcg cttccagcgc tcgcgctgct ggggaacgcc
     2221 gcgaagcgtc ttcgagtaga agtcgaagct cgcctggtcg aaggccttgg gaagataggc
     2281 cgcgtggctg ctgacgaagt ggaaggcgag atagtccttc caggtcgaga gcggcaccgt
     2341 ctcgagcatc ttgcccgtct cggtgatcgc gctcttctcg gcggcgatga ccgtcgggac
     2401 gctgcccagg cccgccgcct cgaggaagac cggccattcc agctgcggag cgagctcggc
     2461 cagctgctgg cggttcatcg ggttgtagat ctgcttgatg tcgcgctggc gcgccggctc
     2521 ccaatggacc ttggcgagcg cggtctcgag tgcgatgatc cggtcggcct tcgcttccgc
     2581 gtcggcgatc ccggcaagct gctgcatctt cacgacatag gcgcgatagg ccttgcggat
     2641 gaggtcgtac ttctcgccct cgagcagata ataatcgcgg ttggggaggc ccaggccgcc
     2701 ctggccggcg gcggcgatgt agcgggtggg atcggccgga tcgggaagga tccccactcc
     2761 gatcgggctc atcacgcccg gctcggcgaa ggcgcggatg aggtcggtgc ggttgttgat
     2821 cgaagcgaag cgggcgagat agggcttgag cggagcggtt ccggcggcct cgattcccgc
     2881 ctcgtccatc cagctggcat aatagtcgcc gacctgctgg ccgatcttgc cggacttggc
     2941 gggatccctg gccaggtcct cgacgatcgc gcgcacctgg cgctcggcct cgtcggcgag
     3001 aagcacgccc acgccggcgc tggtgcggtc ggcggcgatc tgggtggtct tgtcccaatt
     3061 gccgttgacg aaatgccaga agctgtcgcc cggcttcacc gaccggtcca tg
//
LOCUS       DASVQZ010000345        12703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_363446, whole genome shotgun sequence.
ACCESSION   DASVQZ010000345 DASVQZ010000000
VERSION     DASVQZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363446"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VF547_09840"
     CDS             <1..680
                     /locus_tag="VF547_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263161.1"
                     /translation="CEFVGNQSAINAGYGERYQGYLRVLDCRSSNPAGGRYHIILHWT
                     GALIQGGLHDAGTGQICLSWQSEKAGSDVTVRDCEFRTSGLYGVIHYWPGNLVRMERV
                     RIIGTHRDVASHGEVLGFHPDPGGGRKNVLRDCEIFIPGVRKSDATNYDYEVLLYHTL
                     AEGNLFRTDLPAGGDRHFAIGYGRGSTARGERFRGTAPGPHDSIRPYHNSNHDTRMPY
                     SAPEQTT"
     gene            779..2398
                     /locus_tag="VF547_09845"
     CDS             779..2398
                     /locus_tag="VF547_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263162.1"
                     /translation="MDIKVAVIADGTAVPRFGLEALNALAGCDRVTVFACTNTRFTRK
                     PVRHGAYYLLNLFTVRNRWSRPVPIGSGSKRVERVVEFESGWEGAWQTLPDAVVDALR
                     EGGFDVAIKFGMGLLRIPPAERLPVPILSYHHGDPDHFRGRPAGFWETMAGRPVMGQI
                     VQVLSNRLDAGRVVAFAETRVLPYSYRGTLAEAYRHSSLLINEAVRNATAGTYLPKPC
                     QGKNYRLPSNADVVRFAARTSWQAIRRVAYGAALEKKWEVSLAGCGREALDEVVRGAA
                     FPAPERWRTIPVGPRYAFYADPFFSDAPGAILVEALNRRSGLGELVLVEGDRHRQVSD
                     GRGHYSYPSTIDVDGRQLILPEICQWSSTRLFELAGDRLSEVAPLRVAGDPRIADPTL
                     IAHEGRFYLFGSPVAYGTNVLGLWSAACLDDLFELHPLSPVRISPQGGRMAGALLRLE
                     GRLIRFGQSFERYYGDGIVAFEIEALSAQTYRERRIGEIRFGGDRIGPHTLNARDGEL
                     VFDWYRHKPDLLAGARRLVARLRGGSTLPAR"
     gene            complement(2407..3552)
                     /locus_tag="VF547_09850"
     CDS             complement(2407..3552)
                     /locus_tag="VF547_09850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8263163.1"
                     /translation="MAAMNSSSPPAESAGKSIFLLGRSLGIGGAERQMVALAVGLNAL
                     GYKVTAGLFYCGGVLDRELTDRGIPIVDLGKKGRWDVAGFLFRLVRALRSTRPDVVYA
                     FLGTANTVATAARPFAPRFRLLWSIRASNVDLDHYGWVSHLGYRVERALARSPDLIVS
                     NSIAGADYAVAQGFPRDLIVVVPNGIDTDRFRPDPALRREARARWGLGDGEVAVGMLA
                     RIDPVKDHSSFLRAAALVARKRPDVRFLCIGDGQEDYVRRLKAQAEEAGISDRVLWPG
                     AARDPVPALNALDLACSSAETEGFSNAVAEAMACGLPCVVTDVGDCALIVGDTGVVVP
                     PKDSDALAAGLMEALERLGSDAGSRARERVVSEFSVQRMVERTIDCF"
     gene            complement(3518..4360)
                     /locus_tag="VF547_09855"
     CDS             complement(3518..4360)
                     /locus_tag="VF547_09855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8263164.1"
                     /translation="MKQTETDWARSIERGERFAFGQNWARFLRNLDEGRIERAEKSLQ
                     DMLGAETLEGKRFVDIGSGSGLFSLVARRLGAEVHSFDFDSQSVACTTALRDRYFPDD
                     PKWRVEQGSVLDRAYLATLGRFDIVYSWGVLHHTGAMWEALGNVAPLVAEGGTLFIAI
                     YNDQGRSSRLWTKVKRAYVRAPGPLKWGVLLPAMVRLWGPSTLRDLGRGKPFRTWRDY
                     RNAGVRGMDPWRDVVDWVGGYPFEVAKPEEILDFYRERGFELRRMTTCAGGHGCNEFV
                     FAAR"
     gene            complement(4380..5213)
                     /locus_tag="VF547_09860"
     CDS             complement(4380..5213)
                     /locus_tag="VF547_09860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8263165.1"
                     /translation="MSAKAYSRLYEGRKLAQAYPDLDAIATEHFAATPMGAGTIADTL
                     EIIERLVDLPETSKSVAIVGCGPLPASLKAIQGLGYDAVGVEPVPGFVATAREFLGES
                     GAQVVEGAAEKLPFEDESLAVVLLESVLEHVDSPSKSLAEAYRVLAPGGVAFIETSNR
                     RRFHPLGKNGEYNLPFYNFYPKTVKEAIVHHHLHFNPKLANYSTRPAVHWFTYADLCA
                     RGREAGFHRFYARFDGWDTDDPSAGSRLRRAILRMIKYSPFFRTLALLQYGGAVFMLK
                     R"
     gene            complement(5331..6092)
                     /locus_tag="VF547_09865"
     CDS             complement(5331..6092)
                     /locus_tag="VF547_09865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX8263166.1"
                     /translation="MINLARFVRNLGSRDRIRRLFALQAIANRLIPDYRLSWYQLDWW
                     EDPDFNAYLERFGERRGLNTHRRWMLWQLLRLTAGVKGDTAECGVFQGSSSWLICSAN
                     RGRGRTHHLFDSFEGLSKPGAEDGTYWTPGDLAAGEQIVLEKLSPFADELSFHKGWIP
                     DRFADVEEKTFSFVHVDVDLYQPTRDSIAFFYPRLAPGAILLCDDYACSTCPGATQAA
                     DEFLADKPEKMMALDAGGGFFIKGTAVAPPGRLAA"
     gene            complement(6089..7402)
                     /locus_tag="VF547_09870"
     CDS             complement(6089..7402)
                     /locus_tag="VF547_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX8263167.1"
                     /translation="MGRLRGAHANHKRIAAGALWIAVLVAAAKLFVAAREMALAWRYG
                     VSATVDAYQFAFTIITWLPLILAALASAVLVPRLVALHRDPAAYRRFTAELNGTALAV
                     GLAVALLSWFLAPRIVGLVATDFDPDALRLARSMATQLSPVAFLVTAAGYLAARLQAR
                     ERYAYSFAEAVPALAIALFVILAPGRDGGAVLIWGTLVGYVAQALWLADLTRRSDPPL
                     LQLGLRHRAPEWKLVYMPLLVMAAGQVVLTVTNPVDVAFAAQLGEGAVATLGYANRIV
                     GLIVGFSLVVVGRALLPVLSGAVADGEHALGTRQSLQWAMLLFGIGAAAVIAGWPLAP
                     TAVALIFERGAFDSADSAVVTHVLRYGLLQLPFVFGGLVLVQWIAASGRYGVLLAVAC
                     LALVVKITMNLLLLQPLGLAGLMAATAGMYAVSFLGQLAFVMRKR"
     gene            complement(7438..9450)
                     /gene="asnB"
                     /locus_tag="VF547_09875"
     CDS             complement(7438..9450)
                     /gene="asnB"
                     /locus_tag="VF547_09875"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8263168.1"
                     /translation="MCGFAGFLGGAALFGSGRAAQIATAMRECIRHRGPDHGGLWLDA
                     EAAIAFAHRRLAIVDLSAAGDQPMHSVSGRYVTVYNGEIYNHLDLRRQLEEEGHRFEW
                     RGHSDTETLLAAIDVWGVRGALQRSIGMFALALWDRRERLLILARDRLGEKPLYYGWQ
                     GEGPGATFLFGSELKALRRHPAFAGDIDRDSLTLLMRYAYVPAPHSIYRGIRKLMPGC
                     IATLRAGTREPEIEAYWSAAEVAVQGVRQPFEAGPEEAVESLERLLMDAIGQQMIADV
                     PLGAFLSGGVDSSTVVALMQAQSARPVKTFSIGFHEAEYNEATHAKAVAEHLGTDHTE
                     LYVTPAEAMQVIPQLPEMYDEPFADSSQIPTYLVSRLARRNVTVSLSGDAGDELFAGY
                     NRYAMTSAYWRRLSMLPRPLRGTLAGALTSVSPRAWNRLAGAVGPILPNSLRMRMPGD
                     KIHKGAGVLKSADVAEVYHGLVSNWRDPEALVVGGREPPTLLTGKAPDLAGLSEIERM
                     MALDMITYLPDDILAKVDRAAMSVSLETRVPLLDHRVVEFAWRLPSEYKIRDGQTKWA
                     LREVLYRHVPRSLIERPKMGFGIPVGDWLRGPMRAWAEALLDPDRLAREGYFRPDAIR
                     RMWQAHLQRDVNEQYRLWIVLMFQAWLEAERAGADLSVEPAPRAVA"
     gene            complement(9451..10635)
                     /locus_tag="VF547_09880"
     CDS             complement(9451..10635)
                     /locus_tag="VF547_09880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8263169.1"
                     /translation="MIILHIITDLTTGGAELMLERLVGRHSREGRYRHRVISLRTLGQ
                     VGPRLQSAGIEVEAMGMTGPTGLAAGFVRLVRRIRRIDPAIVQTWMYHSDLIGGLAAR
                     LAGHRRILWGVRVMDIMPAMGVSRSVLLIRRLCARLSRTVPRRILYVAESARIVHEAL
                     GYDPAKAAVIPNGYLVPSEDEAASARRRKREELGLAEDSILIGSGGRFSPQKGYRGFV
                     EAAAAIVRGFPQARFVLFGRGVAWENEELAGWIRDAGLADRFHLLGERRDVLDWLAAL
                     DIFTLFSIGEGFPNIVAEAMSVAVPVVVTDVGDAALLVDDTGIVISPGDLKGLTAALE
                     GLIAAGEGERRRLGGAGRQRVDAVFSLTAVAERYAKLYEEMAGEDRPRARAPGSDGLE
                     LK"
     gene            10761..11906
                     /locus_tag="VF547_09885"
     CDS             10761..11906
                     /locus_tag="VF547_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8263170.1"
                     /translation="MASPPTSRPAAAQRVVVFGSFPWSLINFRGPLIAELVARGHEVF
                     ALAPDIDEDVAGQVRALGAEPVSIRLGRTSLNPFGALATLKDLARTFRSIAPDVVLAY
                     TIKPIILGAVAARAARVPRFVCLVTGLGYAFTGGREPKRLFSRAVGKRMYRRAFARAS
                     LAVFQNPDDLGEFRRLGLLPPTLRTALINGSGVDIGHFSPAPLPAGASFLLIARLLKD
                     KGIREFGRAAARLKAERPDVRISLVGFFEPSANGIGKAEIDGLVAAGIEYLGPQSDVR
                     PAIAAHSVYVLPSYREGTPRSVLEAMAMGRPAITTDAPGCRETVADGVNGFLVPVKDW
                     EAVYRAMLRFVDEPGLIARMGAEARRVAEEKFDVRKVNAALLAEAGL"
     gene            complement(11911..12519)
                     /locus_tag="VF547_09890"
     CDS             complement(11911..12519)
                     /locus_tag="VF547_09890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8263171.1"
                     /translation="MGESLKRACDVILGFAAFLFCIPVILLIAAILLAVDGRPVMFRQ
                     VRIGQGGESFTIFKFRTMRNLYGPSGELLPDEDRTTAFGAFLRRFRLDELPEFWLVFT
                     GKMSLVGPRPLPQYVLEGLPGAAERSTMRPGLTGLAQVSGNIQLTNREKFAVDVYYGR
                     HRTLGLDFKIMFQTLATLAFGGSRDEALIARALAEWDPGEPV"
     gene            complement(12524..>12703)
                     /locus_tag="VF547_09895"
     CDS             complement(12524..>12703)
                     /locus_tag="VF547_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263172.1"
                     /translation="RIKMAKERFLPFDELEPFLARLKDGIVSVDRSTVRASLRDLVAP
                     DQGQIADNVTWLRRA"
BASE COUNT         2041 a         4366 c         4452 g         1844 t
ORIGIN      
        1 gctgcgagtt cgtcggcaac cagtccgcca tcaatgccgg ctatggcgaa cgctaccagg
       61 gctatctgcg agtcctcgac tgccggtcat cgaacccggc gggcggccgt taccatatca
      121 tcctccactg gacgggagcg ctgatccaag gcgggctgca cgacgccggc accggccaga
      181 tctgcctgtc ctggcagagc gaaaaggccg gcagcgacgt gaccgtgagg gactgcgagt
      241 tccgaacgtc ggggctctac ggagtgatac attactggcc ggggaacctg gtccggatgg
      301 agcgcgtcag gatcatcggc acgcatcgag acgtggcgtc ccacggcgag gtgctcggct
      361 tccacccgga tccgggcggc gggcgcaaga acgtgctccg ggactgcgag attttcatcc
      421 ccggggtccg aaaatcggac gccaccaatt acgattacga ggtgctcctc tatcatacgc
      481 tcgccgaggg aaacctcttc cggaccgacc tcccggccgg gggcgaccgg catttcgcga
      541 tcggctacgg gaggggcagc accgctcggg gcgagcggtt ccggggcacg gcgccggggc
      601 cgcacgacag catccggccc taccacaatt cgaaccacga cacgcggatg ccctattccg
      661 cccccgagca gacgacctga gcaagccggc cgggtccctc gtcatggacc gcggccttgg
      721 acaggcgcct ccagtccgct agtcgcgcgg gcgacgacat cggaaagctt ggcggggaat
      781 ggatatcaag gtcgcagtga tcgccgacgg caccgccgtg ccgcgctttg gcctcgaggc
      841 gctcaacgcc ctcgcgggct gcgaccgggt gaccgtcttc gcctgcacca acacccgatt
      901 cacgcgcaag ccggtccggc acggcgccta ttatctgctc aacctgttca cggtgcgcaa
      961 ccgctggagc cggccggtgc cgatcgggtc ggggagcaag cgggtcgagc gggtcgtcga
     1021 gttcgagtcc ggatgggagg gggcctggca gaccctgccc gacgcggtcg tcgacgcgct
     1081 gagggagggc ggcttcgacg tcgccatcaa gttcggcatg ggcctgctga gaatcccgcc
     1141 ggccgagcgt ctgccggtgc cgatcctctc ctatcatcac ggcgatccgg accattttcg
     1201 cggccggccg gccggcttct gggagacgat ggccggccgc ccggtgatgg ggcagatcgt
     1261 ccaggtgctg agcaaccggc ttgacgcggg cagggtcgtg gccttcgccg agacgcgcgt
     1321 tctgccctat tcctaccggg ggacgctcgc cgaagcctat cggcattcca gcctgctgat
     1381 caacgaggcc gtccgcaacg cgacggccgg gacctacctg cccaagccct gccaggggaa
     1441 gaattaccgg cttccctcca atgccgacgt ggttcggttc gcggctcgca cctcgtggca
     1501 ggcgatccgg cgcgtcgcct acggcgcggc gctggagaag aagtgggagg tgtccctagc
     1561 cggctgcggc cgcgaggcgc tggacgaggt ggtgcgcggc gccgccttcc cggcgcccga
     1621 gcggtggcgg acgattcccg tcggtccccg ctacgccttc tatgcggacc ccttcttcag
     1681 cgacgctccg ggcgcgatcc tggtcgaggc cctcaaccga cggtcgggcc tgggcgagct
     1741 ggtgctggtc gaaggcgacc gccaccgcca ggtctcggac gggcgcggcc attattccta
     1801 tccgtcgacg atcgacgtcg acggccggca gctgatcctg cccgaaatct gccaatggtc
     1861 gtcgacccgg ctcttcgagc tggccggcga caggctgagc gaggtggcgc cgctgcgcgt
     1921 ggcgggggac ccgcggatcg ccgacccgac cctgatcgcc catgagggac gcttctatct
     1981 gttcgggagc ccggtcgcct acggcaccaa tgtcctgggc ctgtggagcg ccgcctgcct
     2041 cgacgacctt ttcgagctcc atccgctcag cccggtccgg atctcccccc agggcgggcg
     2101 gatggccggc gccctgcttc ggctcgaggg acggctgatc cgcttcgggc agagcttcga
     2161 gcgctactac ggggacggca tcgtcgcctt cgagatcgaa gcgctgagcg cacagaccta
     2221 cagggagcgc aggatcggcg agatccgctt cggcggcgac cgcatcggtc cgcacacgct
     2281 gaacgccagg gacggggagc tggtcttcga ctggtaccgg cacaagcccg atctcctggc
     2341 cggggcgcgg cggctggtgg cgcggctccg gggcggctcg actctcccgg cgcgctgacc
     2401 ggggcctcag aagcagtcga tcgtccgttc caccatcctc tgcaccgaga actcgctcac
     2461 gacccgctcg cgcgcccgcg atccggcatc ggagccgagc cgctccagag cctccatcag
     2521 cccggcggcg agcgcgtccg aatccttggg cgggacgacg actccggtat cgccgacgat
     2581 gagggcgcaa tcgccgacgt cggtcacgac gcaggggagg ccgcaggcca tcgcctcggc
     2641 aacggcgttc gagaagccct ccgtctcggc ggaggagcag gcgaggtcga gcgcattgag
     2701 ggccggcacg ggatcgcgcg ccgccccggg ccacagcacc cggtccgaga tcccggcctc
     2761 ctccgcctgc gccttgagcc ttcgcacata atcctcttgc ccgtcgccga tgcacaggaa
     2821 gcggacgtcg ggccgctttc gggcgacgag ggcggcggcg cgcaggaagc tggaatgatc
     2881 cttcaccggg tcgatccggg cgagcattcc gaccgccacc tcgccgtcgc cgaggcccca
     2941 tcgcgcccga gcctcgcgcc ggagcgccgg gtcgggccgg aagcggtcgg tgtcgatgcc
     3001 gttcggcacg acgacgatga ggtcgcgcgg aaagccttgg gcgacggcat aatcagcgcc
     3061 ggcgatcgag ttggagacga tgaggtccgg cgagcgggcg agggcgcgct cgacgcggta
     3121 gccgaggtga gagacccagc cgtaatggtc gagatccacg ttcgacgccc ggatgctcca
     3181 cagcagccgg aaccgcggcg cgaacggcct cgccgcggtc gcgacggtgt tggcggtgcc
     3241 gaggaaggca tagacgacgt cggggcgggt ggagcgcagc gcccggacca gcctgaacag
     3301 gaatccggcc acgtcccagc ggcccttctt gccgaggtcg acgatcggta tgccgcggtc
     3361 ggtgagctcg cggtcgagca ctccgccgca atagaacagc cctgccgtca ccttgtagcc
     3421 gagcgcgttc agcccgacgg cgagcgccac catctggcgt tccgcgccgc cgatccccag
     3481 cgaccggccg agaaggaaga tcgatttgcc ggccgactca gcgggcggcg aagacgaatt
     3541 cattgcagcc atggcctccg gcgcaggtcg tcatgcgccg aagctcgaag ccgcgctcgc
     3601 gatagaaatc cagtatctcc tcgggcttgg ccacctcgaa cggatagccg ccgacccagt
     3661 cgacgacgtc ccgccacggg tccatgccgc ggacgcccgc attcctgtag tccctccagg
     3721 tgcggaaggg cttgccgcgg ccgaggtcgc gaagcgtgga cggcccccag aggcgcacca
     3781 tggcggggag caggacgccc catttgagcg ggccgggcgc gcgcacgtag gcgcgcttga
     3841 ccttggtcca gagacggctc gagcggccct ggtcgttgta gatggcgatg aacagggtgc
     3901 cgccctcggc gaccagcggc gcgacgttgc cgagcgcctc ccacatcgcg ccggtatggt
     3961 ggagcacgcc ccaggaatag acgatgtcga agcggccgag cgtggccaga taggcgcggt
     4021 cgaggaccga gccctgctcg acccgccact tcggatcgtc ggggaaatag cggtcccgaa
     4081 gcgccgttgt gcaggccacg ctctggctgt cgaaatcgaa cgaatggacc tcggcgccca
     4141 gccgtcgcgc gaccaggctg aacaggccgc ttcccgatcc gatgtcgacg aacctcttcc
     4201 cttcgagcgt ctcggcgccg agcatgtcct gcagggattt ctcggcacgc tcgattcggc
     4261 cctcgtccag attgcgcagg aaccgcgccc aattctggcc gaaggcgaac cgctcgccgc
     4321 gctcgatcga gcgggcccag tcggtttccg tctgcttcac aatcgattgc gacactcgat
     4381 cacctcttca gcatgaacac ggcgccgcca tattgaagga gagccagggt acggaaaaac
     4441 ggggaatatt tgatcatcct gaggatcgcc cggcgaaggc ggctgccggc cgacggatcg
     4501 tccgtgtccc aaccgtcgaa gcgggcgtag aagcggtgga agcccgcctc ccggccgcgg
     4561 gcgcacaggt ccgcataggt gaaccagtgg accgcgggcc gggtcgaata gttcgccagc
     4621 ttcggattga agtggagatg gtgatggacg atcgcctcct tcaccgtctt cgggtagaag
     4681 ttgtagaatg gaaggttgta ctcgccgttc ttgccgagcg gatggaaccg ccgccggttc
     4741 gacgtctcga tgaacgcgac gcctcccggg gccaggacgc gataggcctc ggccagcgac
     4801 ttggagggcg aatcgacatg ctcgagcacg ctctcgagca ggacgacggc gagcgattcg
     4861 tcctcgaacg gaagcttctc cgccgcgcct tccacgacct gcgcgccgct ttctcccagg
     4921 aactcccggg cggtggcgac gaacccggga accggttcga ccccgacggc gtcgtagccc
     4981 aggccctgga tcgccttgag cgaagcgggc agggggccgc agccgacgat cgcgaccgac
     5041 ttgctggtct cgggcaggtc gaccagccgt tcgatgattt ccagtgtgtc cgcgatcgtc
     5101 ccggcgccca tcggcgtcgc ggcgaaatgc tccgtcgcga tcgcgtcgag atcgggatag
     5161 gcctgcgcca gcttccgtcc ttcgtacagc ctggaatagg ctttcgcgct catcttcttt
     5221 ccttggcttg gtgcgcccgc ggcgaacgtc ccggtctgct ccggcttccg gccggcgggc
     5281 cttagcggac gctgggcggc aatcctagct cttgtcctcc gcggcttcga tcaggcggcc
     5341 agccggccgg gcggcgccac cgccgtcccc ttgatgaaga agccgccgcc ggcatcgagc
     5401 gccatcatct tctccggctt gtccgcgagg aactcgtcgg ccgcctgggt ggcgccgggg
     5461 caggtcgagc aggcataatc gtcgcacagc aggatcgcgc cgggggcgag gcgcgggtag
     5521 aagaaggcga tgctgtcgcg ggtcggctgg tagaggtcga cgtcgacgtg gacgaaggag
     5581 aaggtcttct cctcgacgtc ggcgaagcgg tcggggatcc accccttgtg gaaggagagc
     5641 tcgtccgcga agggcgagag cttctcgagg acgatctgct ctccggcggc gagatcgccc
     5701 ggcgtccagt aggtgccgtc ctccgccccc ggcttgctca ggccctcgaa gctgtcgaac
     5761 agatggtggg tccggccgcg gccgcgattg gccgagcaga tgagccatga ggaggagccc
     5821 tggaaaacgc cgcactcggc ggtgtcgcct ttcacgccgg cggtgagccg gagcagctgc
     5881 cacagcatcc accggcgatg cgtgttgagg ccgcgccgct cgccgaaccg ctcaagatag
     5941 gcgttgaaat ccgggtcttc ccaccaatcc agctggtacc agctcaggcg gtagtcgggg
     6001 atcagccggt tggcgatcgc ttgcagggca aacaggcgcc ggatgcggtc gcgggatccg
     6061 aggttccgca cgaacctggc taagttgatc atcttttcct catgacgaag gcgagttggc
     6121 ccaggaagct gacggcgtac attccggcgg tcgccgccat cagtcccgcg aggcctaagg
     6181 gctggagaag cagcaggttc atagtgattt tcaccaccag cgcaaggcag gcgacggcca
     6241 gcaggacgcc gtaacgcccg gaagccgcga tccactggac cagcaccagc ccgccgaaaa
     6301 cgaagggcag ctgaagaagc ccgtaacgca ggacgtgggt caccacggcg ctgtccgccg
     6361 agtcgaacgc gccccgctcg aagatcagcg cgacggcggt cggggcgaga ggccagccgg
     6421 cgatgacggc ggcggctccg atcccgaaca gcagcatcgc ccattgtagc gactggcgcg
     6481 tgcccagcgc atgctcgccg tccgcgaccg ccccggaaag gaccggaagc aaggcgcggc
     6541 cgacgaccac caggctgaag ccgacgatca ggccgacgat ccggttggcg tagcccagcg
     6601 tcgcgaccgc gccttcgccg agctgggcgg cgaacgccac gtccaccgga ttggtgaccg
     6661 tcagaaccac ctgtcccgcc gccatcacca gcagcggcat gtagaccagc ttccattccg
     6721 gagcgcggtg gcgaaggccg agctggagca gcggcggatc ggagcgccga gtcaggtcgg
     6781 ccagccagag ggcctgggcg acatatccga ccagcgtccc ccagatcagc acggcgccgc
     6841 cgtcgcgccc gggagcgagg atcacgaaca gggcaatggc cagcgccggc accgcctcgg
     6901 cgaagctgta ggcatagcgc tcgcgggcct ggagccgcgc ggccagatag ccggcggcgg
     6961 tgaccaggaa cgccaccggc gaaagctgag tcgccatcga gcgggccagc cggagcgcgt
     7021 cgggatcgaa gtccgtcgcg accaggccga cgatgcgcgg cgcgaggaac cagctcagca
     7081 gggcgacggc cagcccgacc gccagcgccg tgccgttgag ctccgccgtg aagcggcgat
     7141 aggcggccgg gtcgcgatgc agggccacca gccgcggcac caggaccgcg gaggcgagcg
     7201 cggcgaggat cagcggcagc caggtgatga tcgtgaaggc gaattgatag gcgtcgacgg
     7261 tggcggagac cccgtagcgc cacgcgagcg ccatttcccg cgcggccacg aacagcttcg
     7321 cggcggcgac gagcacggcg atccacaagg cgccggcggc gatccgctta tggtttgcat
     7381 gggcgccccg gaggcgcccc atcaacgtgc cgatcaaggc cctgtcccgg ctttcgctca
     7441 ggcgaccgcg cgcggcgccg gctcgacgga caggtcggcg ccggcgcgct ccgcctcgag
     7501 ccaggcctgg aacatcagca cgatccagag ccggtactgt tcgttgacgt cgcgctggag
     7561 atgcgcctgc cacatgcgcc ggatcgcgtc gggccggaaa tagccttccc gggcgagccg
     7621 gtcgggatcc agcaaggctt cggcccaggc ccgcatcggc ccgcgcagcc agtcgccgac
     7681 cggaatcccg aagcccattt tcggccgttc gatcagcgac ctcggcacat gccggtagag
     7741 cacctcccga agcgcccatt tggtctgccc gtcgcggatc ttgtactcgg acgggagccg
     7801 ccaggcgaat tcgacgacgc ggtggtcgag cagcggcacg cgcgtctcga gcgacacgct
     7861 catcgccgcc cggtcgacct tcgcgaggat gtcgtcgggc aggtaggtga tcatgtcgag
     7921 cgccatcatc cgctcgatct ccgagaggcc ggccagatcc ggcgccttgc cggtcaggag
     7981 ggtcggaggc tcgcggccgc ccaccaccag cgcctccgga tcgcgccagt tcgagaccag
     8041 gccgtgatag acctcggcga cgtccgcgct cttgagcacg ccggcgccct tgtggatctt
     8101 gtcgcccggc atccgcatcc tgagcgaatt gggcaggatc ggaccgaccg ccccggcaag
     8161 ccggttccag gcgcgggggg agacgctggt gagagcgccg gcgagggtgc ctcgcagcgg
     8221 ccttggaagc atcgacaggc gacgccaata ggccgaggtc atcgcatagc ggttgtagcc
     8281 ggcgaacagc tcgtcgcccg cgtccccgga gagggacacg gtaacgttcc gccgggcgag
     8341 ccgggacacg aggtaggtgg ggatctgcga cgaatcggcg aacggctcgt cgtacatctc
     8401 cggaagctgc gggatgacct gcatcgcttc cgcgggcgtg acgtagagct cggtatggtc
     8461 ggtgccgaga tgctcggcga ccgccttggc atgcgtcgcc tcgttatatt ccgcctcgtg
     8521 gaagccgatc gagaaggtct tcaccggccg cgccgactgc gcctgcatga gcgcgacgac
     8581 cgtcgaggaa tcgactccgc cggagaggaa ggcgccgagc gggacgtcgg caatcatctg
     8641 ctggccgatc gcgtccatca acagccgctc gagcgattcg accgcctctt cggggcctgc
     8701 ctcgaacggc tggcgcacgc cctgcacggc gacctcggcc gccgaccaat aagcctcgat
     8761 ctccggctcg cgggtgccgg cgcgaagggt ggcgatgcag ccgggcatca gcttgcggat
     8821 gccgcgatag atcgaatggg gcgccgggac ataggcgtag cgcatcagca gggtgagcga
     8881 gtcccggtcg atgtcgccgg cgaacgccgg gtgccggcgg agcgccttca gctcggagcc
     8941 gaacaggaag gtggcgcccg gcccctcccc ctgccagccg taatagagcg gcttctcgcc
     9001 gagccggtcc cgggcgagga tgagcagccg ctcgcgccgg tcccacagcg ccagcgcgaa
     9061 cattccgatc gagcgctgga gcgctccgcg gacgccccag acgtcgatcg cggccaggag
     9121 ggtctcggtg tcggaatggc cccgccattc gaagcgatgt ccctcctcct cgagctggcg
     9181 gcgcagatcg agatggttgt agatttcgcc gttgtagacg gtgacgtagc ggccgctcac
     9241 cgaatgcatc ggctgatcgc cggccgccga caggtcgacg atggcgaggc ggcggtgggc
     9301 gaaggcgatc gcggcttcgg cgtcgagcca caggccgcca tgatcgggtc cgcgatgccg
     9361 gatgcattcc cgcatcgccg tcgcgatctg cgccgcgcgc ccgctgccga acagcgccgc
     9421 gccgccaagg aagcctgcaa agccgcacat ctacttaagc tccaatccgt cgctgccggg
     9481 cgcccgcgct cgggggcggt cctcgccggc catctcttcg tacagcttcg cgtagcgttc
     9541 tgccactgcc gtcagggaga aaacggcgtc gacccgttgc cgcccggccc cgcccagacg
     9601 ccgccgctcg ccctccccgg ccgcgatcag cccttcgagg gccgccgtca agcctttgag
     9661 atcgccgggg cttatgacga ttccggtgtc gtcgaccagc agcgcggcat cgccgacgtc
     9721 ggtgaccacg accgggaccg cgacgctcat cgcctccgcg acgatgttgg ggaagccctc
     9781 gccgatcgag aacagggtga agatgtcgag tgcagcgagc cagtcgagca cgtcgcggcg
     9841 ctcgccgagc aggtggaagc gatcggcaag cccggcatcg cggatccagc cggcaagctc
     9901 ctcattctcc caggccaccc ctcggccgaa cagaacgaag cgcgcctgcg ggaagccgcg
     9961 gacgatcgcg gccgccgcct cgacgaagcc gcggtagccc ttctgcgggc tgaaccggcc
    10021 gccgctgccg atcaggatgg aatcctcggc caggccgagc tcctcgcgct tgcgccgacg
    10081 cgccgacgcg gcctcgtcct cagagggcac gagatagccg ttcgggatca ccgccgcctt
    10141 ggccgggtcg tagccgagcg cctcgtgcac gatcctcgcc gattcggcga cgtagaggat
    10201 ccgccggggc acggtccgcg acagccgtgc gcagagtcgg cggatcagca gaacggaccg
    10261 cgacaccccc atggcgggca tgatgtccat gacgcggacc ccccagagga tccgccggtg
    10321 gccggcgagg cgcgcggcaa gcccgccgat caggtccgaa tggtacatcc aggtctggac
    10381 gatggcgggg tcgatccgcc ggatccggcg gacgaggcgg acgaagcctg cggcgaggcc
    10441 ggtggggccc gtcatcccca tcgcctccac ctcgatcccg gcggactgga ggcgcggccc
    10501 gacctggccg agcgtgcgca gcgagatcac ccggtgccgg tagcggcctt cccgggaatg
    10561 gcgcccgacg aggcgctcga gcatgagctc ggcgccgccg gtggtcaggt cggtgatgat
    10621 gtgcaggatg atcatggatg tccccggccc cgcggcgggc cgcccggctc catgcaacag
    10681 gaaggcgcgc ggcggaaggg gcggcgggcg ccggccggcc gcttgcgcgc tcttccgggc
    10741 gaccctagac gcacgcccag atggcttccc ccccgacatc caggcccgcg gcggcgcaaa
    10801 gggtcgtcgt cttcggctcc ttcccctggt cgctgatcaa ctttcgcgga ccgctgatcg
    10861 ccgagctggt ggcgcgaggc cacgaggtgt tcgcgctcgc ccccgacata gacgaggacg
    10921 tcgccggcca ggtccgagcg ctcggcgccg agccggtgtc gatccggctc ggccggacca
    10981 gcctcaatcc gttcggagcg ctcgccaccc tcaaggacct cgcccggacc ttccggtcga
    11041 tcgcgcccga cgtcgtcctg gcctatacga tcaagccgat catcctcgga gcggtcgcgg
    11101 cgcgggcggc ccgggtgccg cgcttcgtct gcctcgtcac cggcctcggc tacgccttca
    11161 ccggcggccg cgagccgaag cggctgttca gccgcgcggt cggcaagagg atgtaccggc
    11221 gggcgttcgc gcgggcgtcc ctcgccgtct tccagaatcc ggacgatctc ggcgaattcc
    11281 gccggctcgg cctgctgccg ccgaccctgc gcacggccct gatcaacggg tcgggcgtgg
    11341 atatcgggca tttctcgccg gcgccgctgc cggccggcgc ctccttcctg ctgatcgcgc
    11401 gcctgctcaa ggacaagggc atacgcgagt tcgggcgcgc ggcggcacgg ctgaaggcgg
    11461 agcggcccga cgttcgaatc tcgctggtcg gcttcttcga gccctcggcg aacgggatcg
    11521 gcaaggccga gatcgacggg ctcgtcgccg ccggcatcga atatctcggt ccgcagtcgg
    11581 acgtgaggcc cgcgatcgcc gcgcacagcg tctacgtcct gccgtcctac cgcgagggca
    11641 cgccccgttc ggtgctcgag gcgatggcga tgggccggcc ggcgatcacg accgacgctc
    11701 cgggctgccg ggagaccgtc gccgacggcg tcaacggctt cctggttccg gtcaaggact
    11761 gggaggcggt gtaccgggcg atgctgaggt tcgtggacga gcccggcctg atcgcccgga
    11821 tgggcgccga ggcgcgccgc gtcgccgagg agaagttcga cgtgcgcaag gtgaacgcgg
    11881 cgctgctcgc cgaggctggc ctgtgagcgc ctagaccggc tcgcccgggt cccattcggc
    11941 gagggcgcgg gcgatcagcg cttcgtcccg gctgccgccg aaggcgagcg tcgccagggt
    12001 ctggaacatg atcttgaagt cgaggcccag cgtccggtgg cggccgtaat agacgtcgac
    12061 ggcgaacttc tcccggttcg tgagctggat gttgcccgag acctgggcga ggccggtcag
    12121 gcccggccgc atcgtcgagc gctccgcggc cccgggcagg ccttcgagga catattgcgg
    12181 cagcggccgc ggcccgacga gcgacatctt gccggtgaag accagccaga attcgggaag
    12241 ctcgtcgagc cggaagcggc gaaggaaggc gccgaaagcg gttgtccggt cctcgtccgg
    12301 cagcagctct ccggacggac cgtagaggtt gcgcatcgtg cggaacttga agatcgtgaa
    12361 cgactctccc ccctggccga tcctgacctg ccggaacatc accgggcggc cgtcgacggc
    12421 caggaggatc gccgcgatga gcaggatgac ggggatgcag aagaggaagg ccgcaaatcc
    12481 aagaatcacg tcgcaggcgc gcttcaggct ttctcccaag ggctcaggcc cgccgcagcc
    12541 aggtcacgtt gtcggcgatc tgcccctggt cgggcgcgac gaggtcgcgc aggctcgcgc
    12601 gcaccgtcga ccggtcgacc gacacgatgc cgtccttcag gcgggcgaga aacggctcga
    12661 gctcgtcgaa ggggaggaag cgctccttgg ccatcttgat ccg
//
LOCUS       DASVQZ010000346         5869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_843052, whole genome shotgun sequence.
ACCESSION   DASVQZ010000346 DASVQZ010000000
VERSION     DASVQZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843052"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2752)
                     /locus_tag="VF547_09900"
     CDS             complement(<1..2752)
                     /locus_tag="VF547_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8263173.1"
                     /translation="MNRSVILALGASVLALAAAAPAGAQTVPEGGEAPTGDITTGAGA
                     DQAGDAESGATQSAEDEGDIIVTAQRRAETLQSVPISVSAFTGEALERQQIENASDLQ
                     QSLPNVTYTKTNFTSSSFTIRGIGDLCTGFSCDSATGIHVNDMPLTSTRLFETEYFDL
                     ERLEVLRGPQGTLFGRNATSGVVNFITARPDLNAIHGSASAEYGNFDSMKLTGMINVP
                     IGGIAGIRLAGYWVNRDGYIRNTFDNSRIDDRDLYALRGTIRIEPADGTRLDLIGYYF
                     HEKDNRSRIQKQLCARDNTGILGCRPDRLEFETVNGNATLAAILTSREFFRINNAALT
                     NFGLGSLYGPDNFFGGVVNPASLRTVRTDFTPTYFAEEKHLMARLEQEIGGSFDLTVT
                     GGWTQQQVDSRTDYNLVAGNSLANNPGLQVLAATAAAPGALFPGGVNPYVQARNALIP
                     NGPGGGVCTSETNLNYTGVFGGFVNRCSPGSTDYDRSQSHSRQYSVEAHLDSDFDGPF
                     NFLIGGIYLDNRFTDSNYYVASFGLDYAAGILGAATALGQRAAGNTAFPNVFLAPPFY
                     NSEVNRFSLKSYGLFGEVYFEVSDALKLTGGLRYSNDKKRQVARVPLLSWPVPFGTTD
                     ANASPFLGNFDADPTVAGNQPYNVARAAFDEITGRFVVDYQVTPDNMIYASYSRGYKS
                     GGINPPINPIFNVPATFEPEIINAFEIGSKNSFSNGRIRLNLSAFYYDYKGLQLSRIV
                     ARTSVNDNTDASIWGAEAEAVFRPSRSLLINLSGSYLKTEIKDLLLSDPRDVSGGRSD
                     TVIIKDVTNASNCAVIPNTAGNAIGANTLVTAFNGALGLAGPVPVPGTNTTGAFSVCD
                     ALAATIANPSAPLRGLFATPTGPLPFTVSQGVQLDLSGNELPQAPRWKFAAGVQYTFR
                     LGN"
     gene            2902..4428
                     /locus_tag="VF547_09905"
     CDS             2902..4428
                     /locus_tag="VF547_09905"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8263174.1"
                     /translation="MAESGKIRSGAPEHFDVVFVGAGLSGIGAAYHLQKGAPGHSYVI
                     LEGREAIGGTWDLFRYPGIRSDSDMYTLGYSFRPWEEAKAIADGPSILRYVNDTADRY
                     DIRRHIRFRHHVKRADWSSAEARWIVEAERTDTGERVRFSCGWLHMCSGYYNYSEVYD
                     PGFAGKESFRGRIVHPQFWPEQLDYRGRKVVVIGSGATAVTIVPEMAKDAAHVVMLQR
                     SPTYMVSRPSEDRVANFLRRVLPSKLAYGIVRWKNVLLQMLFFNLARKRPAKVKEKLL
                     QEVRKLLPEDYDVGTHFTPRYNPWDQRLCLVPDADMFEAISAGRASIVTGEIERFTEA
                     GIRLADGREVAADIVVTATGLKLQLASDIAFAVDGERRDLSKTLSYRGMMFSGMPNLS
                     YSFGYTNASWTLKADLTGGYLARLLNHLRKTGSEIALPVREPGIEEAPFLDFTSGYVQ
                     RALDILPKQGSRKPWKLYQNYALDMVSLKFASVEDGVIRFLPRGAPAAAEATREREAA
                     "
     gene            4425..5237
                     /locus_tag="VF547_09910"
     CDS             4425..5237
                     /locus_tag="VF547_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8263175.1"
                     /translation="MKLAGRTAVITGAASGIGRGTALALAKRGCNLALADLNQEGLAE
                     TAALAEPFAVKVTRHRLDVADRAAVAAFPDEVLAAHGRADLLFNNAGVAIGGTFEQVA
                     EEDFDWLMEINFWGVVRMTRAFLPLLRSSDQARIVNVSSIFGIIAPPGQTAYCASKFA
                     VRAFSDSLRRELEAEGSKIGVTVVHPGGVNTSIAKNARPPRGINDPALEAQKKRFEKF
                     LRMPPGTAGEIIVAGVERERPRVVVGNDARFMTIVERLAPVSYWKLLGRALR"
     gene            5234..>5869
                     /locus_tag="VF547_09915"
     CDS             5234..>5869
                     /locus_tag="VF547_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8263176.1"
                     /translation="MIPFLLAGAGGLAALGGGLAAFSGATARKIEKAVPRDGALIEVN
                     GEKLHVVDRGSGPPIVLIHGLGGQLRNFGRPMVDDLARDYRLVLVDRPGSGYSTRAPG
                     ASARLRVQAETIAELIRILKLDRPLIVGHSLGGALALSLALNHPDVVGGLALIAPLTQ
                     VQSIEDVPPVFRPLVIRSPAVRKAVAWTLSTPMGMMKAEEALKEVFAPEPVP"
BASE COUNT          916 a         1919 c         2037 g          997 t
ORIGIN      
        1 cgttgccgag ccggaaggtg tattggactc cggccgcgaa cttccatctc ggcgcctgcg
       61 gcagttcgtt gccgctgagg tcgagctgga cgccctgcga cacggtgaag ggcagcggcc
      121 cggtcggggt cgcgaacagg cccctcagcg gcgccgaggg gttggcgatg gtggccgcga
      181 gcgcgtcgca caccgagaag gcgccggtcg tgttggtgcc gggcaccggc accggcccgg
      241 cgaggccgag cgctccgttg aacgccgtca ccagggtgtt cgcgccgatc gcgttgcccg
      301 ccgtgttcgg gatgacggcg cagttggagg cgttggtgac gtccttgatg atcaccgtgt
      361 cgctacggcc cccggagacg tcgcgcggat cggacaggag caggtccttg atctccgtct
      421 tgagatagct tcccgacagg ttgatcagaa ggctgcgcga cggcctgaac accgcctccg
      481 cctcggcgcc ccagatcgac gcgtcggtat tgtcgttgac ggaggttcgg gcgacgatgc
      541 ggctgagctg caggcccttg tagtcataat agaaggcgct gaggttcagc cggatgcggc
      601 cgttggagaa gctgttcttg gagccgatct cgaacgcgtt gatgatctcc ggctcgaagg
      661 tcgccgggac gttgaagatc gggttgatcg gcgggttgat gccgcccgat ttgtagcctc
      721 gcgaatagga ggcgtagatc atgttgtcgg gagtcacctg atagtcgacg acgaagcggc
      781 cggtgatctc gtcgaaggcg gcccgggcga cgttgtaggg ctggttgccg gcgaccgtgg
      841 gatcggcgtc gaaattgccg aggaagggcg aggcgttggc gtcggtggtg ccgaagggga
      901 ccggccagct gagcagcgga acccgcgcca cctggcgctt cttgtcgttc gagtagcgca
      961 ggccgccggt gagcttgagg gcgtcgctga cctcgaaata gacctcgccg aacaggccgt
     1021 agctcttcag cgagaaccgg ttgacctcgc tgttgtagaa gggcggcgct aggaagacgt
     1081 tcgggaaggc cgtgttgccc gccgcccgct ggccgagcgc cgtcgccgcg ccgaggatgc
     1141 cggcggcgta atcgaggccg aacgaggcga cgtaatagtt cgaatcggtg aacctgttgt
     1201 cgaggtagat gccgccgatc aggaagttga acggcccatc gaagtcgctg tcgagatgcg
     1261 cctcgaccga atattggcgc gaatggctct gcgagcggtc atagtcggtg ctgcccgggg
     1321 agcagcggtt gacgaagccc ccgaagacgc cggtgtagtt gagattggtc tccgaggtgc
     1381 agacgccgcc gcccggcccg ttgggaatga gcgcgttgcg ggcctggacg tagggattga
     1441 cgccgcccgg gaacagcgcg cccggagcgg ccgcggtcgc ggcgagcacc tgcaggcccg
     1501 gattgttcgc cagcgagttg ccggcgacga gattgtagtc ggtccggctg tcgacctgct
     1561 gctgcgtcca gccgcccgtg acggtgaggt cgaaggatcc gccgatctcc tgctcgaggc
     1621 gggccatcag atgcttttcc tcggcgaaat aggtcggcgt gaaatcggtc cggacggtgc
     1681 gcaggctggc cgggttgacc actccgccga agaaattgtc ggggccgtag aggctgccga
     1741 ggccgaagtt ggtcagcgcc gcattgttga tccggaagaa ctcgcgcgag gtcaggatcg
     1801 cggccagggt ggcgttgccg ttgaccgtct cgaactcgag ccggtccggc cggcagccga
     1861 ggatgccggt attgtcgcgg gcgcagagct gcttctggat gcgcgagcgg ttgtccttct
     1921 cgtggaaata atagccgatc aggtcgagtc gggtgccgtc ggccggctcg atccggatcg
     1981 tgccgcgcag cgcgtagagg tcgcggtcgt cgatccggct attgtcgaac gtgttgcgga
     2041 tatagccgtc gcggttgacc cagtagccgg ccaggcggat gccggcgatg ccgccgatcg
     2101 gcacgttgat catgccggtc agcttcatcg aatcgaaatt gccatattcg gcgctggccg
     2161 agccgtggat ggcgttgagg tcgggccggg cggtgatgaa gttgaccacg cccgaagtcg
     2221 cattgcggcc gaacagcgtg ccctgcggcc cgcgcagaac ctcgagccgc tcgaggtcga
     2281 aatattcggt ctcgaacagg cgggtcgagg tcagcggcat gtcgttgacg tggatgcccg
     2341 tcgccgaatc gcaggagaag ccggtgcaca ggtcgccgat gccgcggatg gtgaagctcg
     2401 acgaggtgaa gttggtcttg gtgtaggtga cgttgggaag gctctgctgg aggtcgctgg
     2461 cattctcgat ctgctggcgc tcgagcgcct cgccggtgaa ggccgagacc gagatcggaa
     2521 cgctctgcag ggtttcggcg cggcgctggg cggtgacgat gatgtcgcct tcgtcctcgg
     2581 cgctctgggt ggccccgctc tccgcgtccc cggcctggtc ggcgccggcg ccggtggtga
     2641 tgtcgccggt cggggcctcg ccgccctcgg ggaccgtctg cgccccggcg ggagccgccg
     2701 cggcgagcgc gagcaccgac gcgccgagcg cgagaataac ggatctattc attgcgtgtg
     2761 ttcctccccg aggcccttgt cggcctattc tcccaggtgc cggttgcatt cggcaacttt
     2821 atggttcttg tcgcacaatc cccttgcgcc ttattggtgt caagcggacg cttgggtgcc
     2881 ggacgggacc ggggggacat tatggcggaa agtgggaaga tccggtcggg cgccccggag
     2941 catttcgacg tcgtcttcgt cggcgccggc ctgtcgggca tcggcgccgc ctaccacctc
     3001 cagaagggcg caccgggtca cagctacgtc atcctcgaag gccgcgaggc catcggcggc
     3061 acctgggacc tgttccgcta tcccggcatc cgctcggaca gcgacatgta cactctgggc
     3121 tacagcttcc gcccctggga ggaggccaag gcgatcgccg acggcccctc gatcctgcgc
     3181 tacgtcaacg acaccgccga tcgctacgac atccgccgcc acatccgctt ccgccaccat
     3241 gtgaagcgcg ccgactggtc ctcggccgag gcgcgctgga tcgtcgaggc ggagcgcacc
     3301 gataccggcg agcgggtccg cttcagctgc ggctggctgc acatgtgcag cggctattac
     3361 aattattccg aggtctacga tcccggcttt gccggcaagg aaagcttccg cggccggatc
     3421 gtccatcccc agttctggcc ggagcagctc gactaccggg gccggaaggt ggtcgtcatc
     3481 ggcagcggcg ccaccgccgt caccatcgtt cccgagatgg cgaaggacgc cgcccacgtc
     3541 gtcatgctgc agcggtcgcc gacctacatg gtgtcgcgcc cgtccgagga cagggtcgcc
     3601 aacttcctgc gcagggtcct tccctcgaag ctcgcctacg gcatcgtccg ctggaagaac
     3661 gtcctcctcc agatgctctt cttcaacctc gcccgcaagc ggccggccaa ggtcaaggag
     3721 aagctcctcc aggaggtgag gaagctgctg cccgaggact atgacgtcgg cacccatttc
     3781 acgccgcgct acaatccctg ggaccagcgg ctctgcctgg tgccggacgc cgacatgttc
     3841 gaggcgataa gcgcgggccg cgcctcgatc gtcaccggcg agatcgagcg cttcaccgag
     3901 gcgggcatcc gcctcgcgga tgggagggag gtggccgcgg acatcgtcgt caccgccacc
     3961 ggcctcaagc tgcagctggc gagcgacatc gccttcgccg tcgacggcga acggcgcgac
     4021 ctgtcgaaga cgctgagcta ccgcggcatg atgttcagcg gcatgccgaa cctgtcctat
     4081 tcgttcggct acaccaacgc ctcctggacc ttgaaggccg acctcaccgg cggatatctc
     4141 gcccgtcttc tcaaccacct gaggaagacc gggtccgaga tcgccctccc cgtccgcgag
     4201 cccggaatcg aggaggcgcc gttcctcgac ttcacctccg gctacgtcca gcgggcgctc
     4261 gacatcctgc ccaagcaggg ctccaggaag ccctggaagc tctaccagaa ttacgcgctc
     4321 gacatggtca gcctgaagtt cgcctcggtc gaggacgggg tcatccgctt cctgccgcgc
     4381 ggcgccccgg cggcggccga ggccacgcgg gaaagggaag cggcgtgaag ctcgcaggcc
     4441 gcaccgccgt catcacagga gccgccagcg gcatcggccg aggcaccgcc ctcgctttgg
     4501 cgaagcgcgg atgcaacctg gccctggccg atctcaacca ggaaggactg gccgaaaccg
     4561 cggccctcgc cgagccgttc gcagtgaagg tcacccgcca ccgtctcgac gtcgccgacc
     4621 gggcggcggt cgccgccttc ccggacgagg tcctcgccgc gcacgggcgc gccgacctcc
     4681 tgttcaacaa tgccggagtc gccatcggcg gaacgttcga gcaggtggcg gaggaggatt
     4741 tcgactggct gatggagatc aacttctggg gggtcgtccg gatgacccga gccttcctgc
     4801 ctctgctgcg cagcagcgac caggcgcgga tcgtcaacgt ctcgagcatc ttcggaatca
     4861 tcgcgccccc cggccagacc gcttattgcg ccagcaagtt cgccgtccgc gccttctcgg
     4921 attcgctgcg ccgcgagctg gaggcggaag ggtccaagat cggggtcacc gtcgtccacc
     4981 cggggggagt caacacctcg atcgcgaaga acgcgaggcc gccgcgcggc atcaacgatc
     5041 ccgccctcga agcgcagaag aagaggttcg agaagttcct gcggatgccg cccggaaccg
     5101 ccggcgagat catcgtcgcc ggcgtcgagc gcgagcggcc gcgggtggtc gtgggcaatg
     5161 acgcgcgctt catgactatc gtcgagcggc tggcgccggt atcctattgg aagttgctcg
     5221 ggagggcctt gagatgatcc cgttcctgct tgccggcgcc ggcggcctcg cggcgctcgg
     5281 cggaggcctc gccgccttca gcggggccac cgcccgcaaa atcgagaagg ccgtcccgcg
     5341 cgacggcgcg cttatcgagg tcaacggcga gaagctccac gtcgtcgacc gcggctccgg
     5401 cccgccgatt gtcctcatcc acggcctcgg cgggcagctc cgcaatttcg gccggccgat
     5461 ggtcgacgac ctcgcccgcg actaccgcct ggtcctcgtc gaccgccccg gctccggcta
     5521 ttccacccgc gcccccggcg cgtccgcccg cctgcgggtc caggccgaga cgatcgccga
     5581 gctgatccgt atcctcaagc tcgaccgtcc cctgatcgtc ggccattcgc tcggcggcgc
     5641 gctggcgctt tcgctcgcgc tcaaccatcc cgacgtggtc ggcggactcg ctctcattgc
     5701 cccgctgacc caggtccaga gcatcgagga cgtgccgccc gtgttcaggc ccctggtcat
     5761 ccggtcgccg gcggtgcgca aggccgtcgc ctggacgctc tcgactccga tggggatgat
     5821 gaaggctgag gaggcgctga aggaggtttt cgcgcccgag ccggtgccg
//
LOCUS       DASVQZ010000347         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_843228, whole genome shotgun sequence.
ACCESSION   DASVQZ010000347 DASVQZ010000000
VERSION     DASVQZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843228"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1634
                     /locus_tag="VF547_09920"
     CDS             297..1634
                     /locus_tag="VF547_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8263177.1"
                     /translation="MEQVQGPPSSSTPLERDARLASTDHKVAPSEIALGVIIGRAAEY
                     FDFFVFAIASVVVFPKVFFPFAEPVTATLYSFAMFSLAFVARPIGSVIFTAVDRRHGR
                     GAKLTIALFLLGFSTMAVSFLPSYAQIGAWSLYLLGVLRVGQGLALGGAWDGLASLLA
                     LNAPVERRGFYAGVAQLGAPLGFMVASTLFSFFLLNLSEPDFLDWGWRYPFFCAFAIN
                     VVAVFARLRLVATDEFSRLLEQGRLEPSPVIPLIRNHSPELLIGALIPLASFALFHLV
                     TVFPISWISLFTQRSAGEFLMVQCSGAFICAGAIVASGLIADQIGRRMLLLLSAGLIA
                     VFAIASTLAPLIVEENAIGQTIYVNAGFALLGLSYGQSSGAVTSRLEQRYRYTGAALT
                     NDLAWLLGAGFAPLIVLYLSARYGLACVGLYLLSGAVTTLLALAVSRSEIRQV"
     gene            1705..>2896
                     /gene="cyoA"
                     /locus_tag="VF547_09925"
     CDS             1705..>2896
                     /gene="cyoA"
                     /locus_tag="VF547_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEX8263178.1"
                     /translation="MRALAIRFTPPTAPRAPRRRSLALKGGLRVAAVLAVAGLLSGCN
                     LVTLNPAGDVAVQQRNLIIAATALMLLVIVPVIALTFIFAWRYRASNTDATYDPEWHH
                     STQLEVVIWTVPLLIIIALGAMTWISTHTLDPFRPLSRLAPNKPVPADAKPLRVEVVA
                     LDWKWLFIYPELGVASLNEMAAPVDVPISFKITSSSVWNTFHVPALAGMIYAMPGMET
                     QLQGVMNKEGEFTGLSAHYSGSGFSRMTFTFRSLGQQGFDEWVAQARASETPLDRAAY
                     LKLDKPSEAEPVRFFGSVENGLYDAALGLCVQPGQMCVQEMHHIDATGGAGRHSEANR
                     KRLDYDRRHLAGGDEPAGATFPASGRPPMSSVQPEGMTPRDQHMSRGGVNERSKTEPD
                     QGQGN"
BASE COUNT          385 a         1100 c          905 g          506 t
ORIGIN      
        1 tggccgcctg gcgctcgagg tcgcgtagct tccgcaggaa cccccagcgg gcgtccggcg
       61 cgctgccggc cggcagggcg cggaacgcct cctcggtgcc gatcacgtgc accagggcct
      121 cgcccgcccg accgcacccg cctacgttgc cctccggcgc gcccggccgg gcgcgccgaa
      181 acatgggcca gctctcccat gacgcatgga tcccacttcg ggatcaggcc atggtcgagc
      241 tgagttcctt ttgccgggct gaactattct cactggaggc gatcacccag atggcgatgg
      301 aacaggttca agggccgccg tcctcctcca cgccgcttga gcgcgacgcc cgcttggcgt
      361 ccaccgacca caaggtcgca ccgagcgaga tcgccctcgg cgtcatcatc ggccgggccg
      421 ccgaatactt cgacttcttc gttttcgcca tcgcctccgt ggtggtgttc cccaaggtgt
      481 tctttccctt cgcggaaccc gtgacggcga cgctttactc cttcgccatg ttctcgctgg
      541 cctttgtcgc ccggccgatc ggctcggtca tcttcacggc ggtcgaccgt cggcacggcc
      601 gtggcgccaa gctgaccatc gcgctctttc tgctcggctt ctccaccatg gcggtctcct
      661 ttctgcccag ctacgcgcag atcggcgcct ggtccctata cctcctaggc gtcctacggg
      721 tcgggcaggg tctcgccctg ggcggcgctt gggacgggct cgcctcgctt ctcgcgctca
      781 acgcgccggt cgagcgccgc ggcttctacg ccggcgtcgc gcaactcggc gcgccgctcg
      841 gcttcatggt ggccagcacc ctgttctcgt tcttcctgct caacctgtcg gagcccgact
      901 tcctcgactg gggatggcgc tacccgttct tttgcgcctt cgcgatcaac gtggtggccg
      961 tgttcgcgcg gctgcggctc gtcgccaccg acgagttctc gcgcctgctc gaacaggggc
     1021 ggctcgaacc ctcgccggtc atccccctga tccggaacca ttcgcccgaa ctcctcatcg
     1081 gcgcgctgat cccgctggcg agcttcgcgc tgttccacct cgtgaccgtg ttcccgatca
     1141 gctggatcag cctgttcacc cagcgctccg cgggcgagtt cctgatggtg caatgctccg
     1201 gagccttcat ttgcgcgggc gcgatcgtgg cctcgggcct gatcgccgac cagatcggcc
     1261 gccgcatgct cctcctgctc tcggccggcc tgatcgccgt cttcgccatc gccagcaccc
     1321 tcgcgcccct gatcgtcgag gagaacgcga tcggccaaac gatctacgtc aacgccggct
     1381 tcgcgctgct cggcctgtcc tacgggcagt cgtcgggcgc cgtgacgtcg cggctcgagc
     1441 agcgctaccg ctacaccggc gcggccttga ccaacgatct cgcttggctg ctcggggcgg
     1501 gcttcgcgcc gctgatcgtg ctgtacctgt cggcccgcta cgggctcgcc tgcgtcggcc
     1561 tctacctgct gtcgggggct gtcaccaccc ttctcgcgct cgccgtgtcc cgctcggaga
     1621 tccggcaggt ctgaacccgc ggacgccgac ccgtccgccc caccttccgc gcctcgccgc
     1681 ccccggacgg cgagggacca aggagtgaga gccctggcca tccgcttcac ccccccgacc
     1741 gccccgcgcg cgccgcgccg caggtcgctc gcgctcaagg gcggcctgcg cgtcgcggcg
     1801 gtgctcgccg tcgcgggact gctttccggc tgcaacctgg tcaccctgaa ccccgcgggc
     1861 gacgtcgcgg tgcagcagcg caacctcatc atcgcggcca ccgcgctgat gctgctcgtc
     1921 atcgtgccgg tgatcgcgct caccttcatc ttcgcgtggc gctaccgcgc ctcgaacacg
     1981 gacgcgacct acgaccccga gtggcaccac tccacccagc ttgaagtggt gatctggacc
     2041 gtgccgctgc tgatcatcat cgcgctcggc gccatgacct ggatcagcac gcacacgctc
     2101 gaccccttcc ggccgctttc ccgcctcgcg cccaacaagc ccgtgcccgc cgacgcgaag
     2161 ccgctcaggg tggaggtcgt cgcgctcgac tggaaatggc tgttcatcta tcccgagctc
     2221 ggcgtcgcca gcctgaacga gatggccgcg ccggtggacg tgccgatctc cttcaagatc
     2281 acgtcctcct ccgtctggaa cacgttccac gtccccgcgc tcgcagggat gatctacgcc
     2341 atgccgggca tggagacgca gctccagggc gtcatgaaca aggaagggga gttcaccggc
     2401 ctgtcggcgc actacagcgg gtcgggcttc tcgcggatga ccttcacctt ccgcagcctt
     2461 ggccagcagg gcttcgacga gtgggtggcg caggccaggg cttcggagac gccgctcgac
     2521 cgcgcggcct acctgaagct cgacaagccg agcgaggcgg agccggtccg ctttttcggt
     2581 tcggtggaga acggcctata cgatgccgcc ctcggcctgt gcgtccagcc cggccagatg
     2641 tgcgtccagg agatgcacca catcgacgcg acgggcgggg cggggcggca tagcgaggcg
     2701 aaccgcaagc ggctcgacta cgatcggcgc caccttgcgg gcggcgacga gccggccggc
     2761 gccaccttcc ccgcttcggg ccgtccgccc atgagctccg tccagcccga gggcatgacg
     2821 ccccgcgatc agcacatgag ccgcggcggc gtgaacgagc gcagcaagac cgagcccgac
     2881 caggggcagg gcaacg
//
LOCUS       DASVQZ010000348         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_72941, whole genome shotgun sequence.
ACCESSION   DASVQZ010000348 DASVQZ010000000
VERSION     DASVQZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72941"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VF547_09930"
     CDS             complement(<1..457)
                     /locus_tag="VF547_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8263179.1"
                     /translation="MASLFDLTGKVAVVTGSSRGIGRAIAEAMAEQGAKVVISSRKAE
                     ACQEVADAINARHPGAAIVVAASISSKEQLQHLANETRRQLGRIDILVCNAASNPYYG
                     PMGGITDDQFRKILENNVIANHWLIQMVAPEMIERKSGSIIIVSSIGGLK"
     gene            complement(574..1656)
                     /locus_tag="VF547_09935"
     CDS             complement(574..1656)
                     /locus_tag="VF547_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX8263180.1"
                     /translation="MKAALLHAPGTPLTLEDVAVSSPGPREVLIRTRACGACHSDLHF
                     IDGAYPAPLPAIPGHEAAGVVEAVGSDVTAVKPGDHVVTCFSAYCGRCEFCVTGRLSL
                     CTDASTKRRKSEAPRLSLNGAPVHQLLNVAGYAEMLLVHENGCVAIDRDMPFDRAALM
                     GCAVVTGAGAVFNTAGVKAGEDVAVIGCGGIGLAAVNAARIAGARHIVAIDPVPAKRA
                     LAEQVGATATFEPSADRLKEQVVELTRGGVHHAIEAVGRISAGELALSLLRRGGTATI
                     VGMMPLTERLSMGAMDLLAEKKLQGSLMGSNRFPLDIPRLVDFYLAGRLDLDAMVAER
                     VGLGEINQAVDKLRTGEAARSVVVFD"
BASE COUNT          262 a          549 c          609 g          274 t
ORIGIN      
        1 ccttgaggcc gccgatcgag ctgacgatga tgatcgagcc gcttttccgc tcgatcatct
       61 ccggcgccac catctggatc agccaatggt tggcgatgac gttgttttcc aggatcttgc
      121 ggaattggtc gtcggtgatg ccgcccatcg gcccgtaata gggattggaa gcggcgttgc
      181 agacgaggat gtcgatgcgg ccgagctgcc gccgcgtctc gttggcgagg tgctggagct
      241 gctccttgga agagatgctc gccgcgacca cgatggcggc gccggggtgc ctggcgttga
      301 tcgcatcggc cacttcctgg caggcttcgg ccttgcggct ggagatcacc accttggcgc
      361 cttgctccgc catcgcttcg gcaatggccc gcccgatgcc gcgcgaggag ccggtgacga
      421 cggcgacttt gccggtgagg tcgaagaggg aagccatgtg ctgttactcc gttgcgtcgt
      481 catgccggac ctgatccggc atccatcttc tttatccacc cgccaaaaca agaaggtgga
      541 ccccggatca agtccggggt gacgttcggg ccctcagtcg aacaccacca ccgaacgtgc
      601 cgcctcgccc gtgcgcagct tgtcgaccgc ctggttgatc tcgccgagcc cgacccgttc
      661 cgccaccatc gcgtcgaggt cgagccgccc ggcgaggtag aagtcgacga gacggggaat
      721 gtcgagcggg aagcggttgg agcccatgag cgagccctgc agcttcttct cggcgaggag
      781 gtccatggcg cccatcgaca agcgctcggt cagcggcatc atgccgacga tggtggcggt
      841 gccgccgcgg cgcaggagcg acaaagcgag ctcgcccgcc gagatgcggc cgaccgcctc
      901 gatcgcgtgg tggacgccgc ccctggtgag ctccaccacc tgctccttca gccggtccgc
      961 ggaaggttcg aacgtcgccg tggcgccgac ctgctcggcg agcgcgcgct tggcgggtac
     1021 ggggtcgatc gcgacgatgt ggcgcgcccc ggcgatgcgc gcggcgttga ccgcggcaag
     1081 gccgatgccg ccgcagccga tcacggccac atcctcgccg gctttgacgc ctgcggtgtt
     1141 gaagacggcg ccggcgccgg tcaccaccgc gcagcccatc agcgccgccc ggtcgaacgg
     1201 catgtcgcgg tcgatcgcga cgcagccatt ttcgtgaacg agcagcatct cggcatagcc
     1261 cgcgacattg agcagctggt ggacgggcgc gccgttcagg gaaaggcgcg gtgcctcgct
     1321 cttgcggcgc ttggtcgagg cgtcggtgca gagcgagagg cggccggtga cgcaaaactc
     1381 gcagcggccg caataggccg agaaacaggt gacgacatgg tcgcccggct tcacggcggt
     1441 aacgtcgctt ccgacggcct cgaccacgcc ggcggcctcg tggccgggaa tggcggggag
     1501 gggcgccggg taggcgccgt cgatgaagtg caggtcggaa tgacacgccc cgcaggcgcg
     1561 ggtgcggatc agcacctcgc gagggccggg gctggagacg gcgacgtcct cgagggtaag
     1621 gggcgtgccg ggggcgtgga ggagggcggc tttcacttca ttcctccccc ggatttccgg
     1681 gggaggggga ccgc
//
LOCUS       DASVQZ010000349         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_270910, whole genome shotgun sequence.
ACCESSION   DASVQZ010000349 DASVQZ010000000
VERSION     DASVQZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270910"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VF547_09940"
     CDS             <1..189
                     /locus_tag="VF547_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX8263181.1"
                     /translation="TLISDSLGRGVKLRVSMNGLRSVEHVGGLDNWLAKAGDEQLSLK
                     ARRLKREIAKTQADTAEA"
     gene            complement(209..1165)
                     /locus_tag="VF547_09945"
     CDS             complement(209..1165)
                     /locus_tag="VF547_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX8263182.1"
                     /translation="MRLLLALLALTLLATFAPVVAPPHAAPPASAWVAARAVALDPSW
                     PGRTRLGALRFLGAWTLTSNDGRFGSISAMHVDGGQVLAFSDAGWMMRFAAPAVEGGR
                     VAARIGALPDGPGSPDRKSARDVEAMWAEGGKAWIAFERINEVWRFDLRSWRAEAHTQ
                     PPILRDWPANRGPEAMARLADGRFILFDEGRGGGGPAALFSGDPAVPGSRALGLRYAA
                     PAGYRPTDAARLPDGSLLVLNRHVSLREGVSAKLVRVRAISGGVLRGEEVADFRPPVR
                     VDNMEALSVTREGGRTILWIASDDNNFPLQRTLLMKFEYAPS"
     gene            1323..1709
                     /locus_tag="VF547_09950"
     CDS             1323..1709
                     /locus_tag="VF547_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263183.1"
                     /translation="MKKFTLAAVAAATAFTAVPAAAQAQYYGGYDRGGYDQGYYGRGD
                     YDRNSDGRYYGRSYRGDRNYNRRCSGTTGTIVGGVAGALLGREVTRGGSRYGRRGSGP
                     TGTILGGALGALAGRAVDKSSCRNRY"
     gene            complement(1972..>3300)
                     /locus_tag="VF547_09955"
     CDS             complement(1972..>3300)
                     /locus_tag="VF547_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX8263184.1"
                     /translation="VSAWIEEKAGGGLDALGLAIDDQAAFAALAAKLLRDLELVEGEQ
                     DAAPDPEEGESGEGGDEAESEEGEDEKEEDPGGGEGEAEIRVEQGDRSDEEGEGDYND
                     QEMGEAEEGVGEDGEEGMMPVRPNRPLSDLPPQFDYRVFTSKYDEVIEAAELCDEAEL
                     GRLRAYLDQQLTHLQGAVTKLANRLQRRLMAQQSRSWDFDQEEGLLDAARLARVVVNP
                     AHSLSYKVERETEFRDTVVSLLIDNSGSMRGRPISIAAISADILARTLERCGVKVEIL
                     GFTTRAWKGGQSRETWLSQGRPPAPGRLNDLRHIVYKAADEPWRRARRNLGLMMREGL
                     LKENIDGEALLWAHNRLIARSEERRILMVISDGAPVDDSTLSVNSGTYLERHLRQVIG
                     WIEARSPVELAAIGIGHDVTRYYERAVTIMDAEQLGGTLVEQLAALFDKP"
BASE COUNT          483 a         1196 c         1123 g          498 t
ORIGIN      
        1 acgctgatct ccgattcgct gggccgcggc gtcaagctgc gcgtctcaat gaacgggctg
       61 cgctcggtcg agcatgtcgg cggcctcgac aattggctgg ccaaggccgg cgacgagcag
      121 ctcagcctca aggcgcgccg cctgaagcgc gagatcgcca agactcaggc cgacaccgcc
      181 gaggcctgag cctttccgcc ctttccggtc aggagggggc gtattcgaac ttcatcagca
      241 aggtccgctg cagcgggaag ttgttgtcgt cggacgcgat ccacaggatc gtccggccac
      301 cttcccgcgt gacgctgagg gcttccatat tgtcgacgcg caccggcggg cggaagtccg
      361 ccacctcctc gccgcgcaag acgccgcccg agatggcgcg gacccgcacc agcttcgccg
      421 aaactccctc ccgcaggctg acgtggcggt ttaacacgag cagcgagccg tcgggcagac
      481 gcgccgcgtc cgtcggccgg taacccgccg gggcagcgta gcgcagcccg agggcgcggc
      541 tgccgggcac cgccggatcg cccgagaaga gcgcggccgg cccgccgccg ccccgcccct
      601 cgtcgaacag gatgaagcgg ccgtcggcca gccgcgccat cgcctccggc ccgcgattgg
      661 ccggccagtc gcgcaggatc ggcggctggg tgtgcgcctc tgcccgccag ctgcgaaggt
      721 cgaaccgcca gacctcgttg atacgctcga aggcgatcca cgccttgccg ccttccgccc
      781 acatcgcctc gacgtcgcgc gccgactttc ggtcggggga ccccggtccg tcgggaaggg
      841 cgccgatccg ggccgccacg cgaccgccct ccacggccgg ggcggcgaag cgcatcatcc
      901 agcccgcgtc gctgaaggcc agcacctgtc ccccgtcgac atgcatggcg gagatgctgc
      961 cgaagcgccc gtcgttgctg gtcagcgtcc aggcgccgag gaaccgcaaa gccccgaggc
     1021 gggtacggcc gggccagctc gggtcgagcg ccaccgccct cgccgcgacc caggcggaag
     1081 cggggggcgc ggcgtgcgga ggggccacca ccggcgcgaa ggtggcgagc agcgtcaggg
     1141 caagaagagc gagcagaagg cgcattcccc gcccgtctag agcgcgcgag gcgccgccgc
     1201 gagcctgaac ggcggctgac gagggcattc aggccggcct cacacgaatc ggcctagaac
     1261 ggcatcattc cagaagggcc cgggggttct cccctcctcc ggcctgccaa aggagagttt
     1321 cgatgaagaa gttcaccctc gccgccgttg ccgccgcgac cgccttcacc gcggtcccgg
     1381 ccgccgctca ggcgcaatat tatggcggct acgaccgtgg cggctacgat cagggctatt
     1441 acggccgcgg cgattacgac cgcaacagtg acggccgcta ttatggccgc tcctaccgcg
     1501 gcgaccgcaa ctacaaccgc cgttgcagcg gcacgaccgg caccatcgtc ggcggcgtcg
     1561 ccggcgcgct cctgggccgc gaagtgaccc gcggcggcag ccgctacggc cgacgcggca
     1621 gcggtcccac cggcacgatc ctgggcggcg ccctcggcgc cctggcgggc cgcgcggtcg
     1681 acaagtcgag ctgccgcaac cgctactaag cgccttgctt aaccctccag tcgcgtagcg
     1741 agggtggggc cggcactcca gcaggagtgc cggccctttg tttttctatc ctccacggat
     1801 ttacggggga ggggggtgta ggcggatcgg catgccgggg gcatgccgcg agccggaacc
     1861 ggctgcgcag cagccgccgg gcgcagcatg gtggaggggc tttttccaag ggagcccctc
     1921 caccgcgctt cgcgcggtcc ccctcccctc gaaatcgagg ggaggacttc gttacggctt
     1981 gtcgaagagc gccgcgagct gttcgacgag ggtgccgccg agctgctcgg cgtccatgat
     2041 ggtgacggcg cgctcgtaat aacgcgtgac gtcgtggccg atgccgatcg cggcgagctc
     2101 gaccggggag cgcgcctcga tccagccgat cacttggcgc aagtgacgct ccagataagt
     2161 gccgctgttc accgacaagg tggaatcgtc gaccggcgcg ccgtcggaga tgaccatcag
     2221 gatgcgccgc tcctcggatc gggcgatcag ccggttgtgg gcccagagca gagcctcgcc
     2281 gtcgatattc tccttgagca gcccctcgcg catcatcagg ccgaggttcc tgcgcgcgcg
     2341 ccgccacggc tcgtcggcgg ccttgtagac gatgtggcgg aggtcgttga ggcgccccgg
     2401 cgcgggggga cggccttggc tcagccaggt ctcgcggctc tgcccgccct tccaggcacg
     2461 agtggtgaag ccgagtatct cgaccttgac cccgcagcgc tccagcgtcc gcgcgaggat
     2521 gtcggcggaa atggccgcga tcgagatcgg ccggccccgc atcgagcccg aattgtcgat
     2581 cagcaggctg accaccgtgt ccctgaactc ggtctcccgc tccaccttgt aggagagcga
     2641 atgggccgga ttgacgacga cgcgggcgag ccgggcggca tcgagcagcc cctcctcctg
     2701 gtcgaagtcc cagctgcgcg actgctgcgc catcagccgc cgctgcaggc ggttggcgag
     2761 cttggtgacc gcgccctgca aatgggtgag ctgctggtcg agataggcgc gcagccggcc
     2821 caattcggcc tcgtcgcaca gttcggcggc ctcgatcacc tcgtcatatt tggacgtgaa
     2881 gacgcggtag tcgaattgcg gcggcaggtc ggacaaaggc cggttcgggc ggacgggcat
     2941 catgccctcc tcgccgtcct cgccgacgcc ttcctcggcc tcgcccatct cctggtcgtt
     3001 atagtcgcct tcgccttcct cgtcggaacg gtcgccctgc tcgacgcgga tctcggcctc
     3061 gccctcgccg ccgcccggat cctcctcctt ttcgtcttcg ccttcctcgc tctcggcctc
     3121 gtcgccgcct tcgccgctct ccccttcctc cgggtccggc gcggcgtcct gctcgccctc
     3181 gaccagttcg aggtcgcgca gcagcttggc ggcgagcgcg gcgaacgcgg cctgatcgtc
     3241 gatcgcgagc ccgagcgcgt cgaggccgcc gccggctttc tcctcgatcc aggcggagac
//
LOCUS       DASVQZ010000350        15424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_940972, whole genome shotgun sequence.
ACCESSION   DASVQZ010000350 DASVQZ010000000
VERSION     DASVQZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 15424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15424
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940972"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VF547_09960"
     CDS             complement(<1..871)
                     /locus_tag="VF547_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8263185.1"
                     /translation="MKADQALAAAVALLIAGCASVPDAPAGAPTARFERFDYRGADPS
                     DSLPAGAGDYRNPILKGFYPDPSLTRVGDDYYLVTSTFSYFPGIPVFHSRDLVGWTQI
                     GNAIDRPGQLDFARLGLSRGVFAPTIEHRDGTFYILNTCVDCGGNFVITATDPAGPWS
                     DPVWLPDLEGGIDPSLFFDEDGSVWILNNGPPEGPPLYEGHRAIWIQRFDAAAKKTFG
                     PRKVLVNGGVDLSKKPIWIEGPHILRKDGFYYLLAAEGGTAEGHSQVVLRSRRPDGPY
                     SAYPGNPILTQRDL"
     gene            complement(1018..2541)
                     /locus_tag="VF547_09965"
     CDS             complement(1018..2541)
                     /locus_tag="VF547_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8263186.1"
                     /translation="MTDQRIRSVVIVGGGTAGWMAAATFAKILTTRYCDVTLIESDEI
                     GIIGVGEATIPQMATFNRMLELDEDDFVRETKGTFKLGIEFHNWGRQGHRYFHPFGSY
                     GLNMEGVSFHAHWLRLHQLGEAPRLDEWSLPALAGERGKFMRPFDAGNSPLSSIAYAY
                     HFDAGLYARYLRRYAEGRGIVRREGKIVEVNLRSDDGFVESVTLENGDRVEADLFIDC
                     SGFRGLIIEQALKAGYEDWSHYLPCNRAIAVPCESVPEITPYTRSTARSAGWQWRIPL
                     QHRIGNGHVFSSDFMTDDEAIDVLMGNLDGKPLADPRVVPFKTGRRKQFWIKNCVALG
                     LAAGFLEPLESTSIWLVQSGLSRLMTMFPDRTFQQADIDRFNRIVLNEYEIVREFLVL
                     HYKATERDDSEFWNYCRTMPIPDRLAEKIRVFEAYGRTFRENDELFNDTSWFAVMIGQ
                     LMKARTYDPVADIMPLDETRRRLRHIAETVHNSAEYMPTHRQFILENCAAPELAEAA"
     gene            complement(2538..4073)
                     /locus_tag="VF547_09970"
     CDS             complement(2538..4073)
                     /locus_tag="VF547_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8263187.1"
                     /translation="MSGRPIRDIVIVGGGTAGWMAAAAMARLRAAGAAITLVESDEIG
                     TIGVGEATIPPIRTFNAMLGLDEDDFVAKTQGTFKLGIEFVDWARPGHRYMHPFGQFG
                     ADIEAVKFHQFWLKCQARGRAAGLEDYNLCAVAAKLGRFQRPVADPRSILSTLNYAFH
                     FDAGLYAAYLRAYAEARGVVRREGKVVDVELRGEDGFVAAVKLAGGLRLEGDLFIDCS
                     GFRGLLIEQALETGYEEWTRWLPCDRAVAIPSTAATEPPPYTRSTALAAGWQWRIPLQ
                     HRTGNGYVYSSAHLSDDEAAATAIANAAGAALGEPRFLRFAAGRRRKLWNRNVVALGL
                     AGGFLEPLESTSIHLVQAGISKLLALFPDRDFDPILAAEYNRLSDLQLEQIRDFIILH
                     YKATERDDSDFWRGCRAMAVPESLSRKIELFRENGRIFRHEDELFSEASWLAVLLGQG
                     VRPRRYDPLADTVDSAGIEATLARMAPFVRKAAEAMPPHSAYIKRHCAAGGDAAKSRG
                     AMA"
     gene            complement(4070..5539)
                     /locus_tag="VF547_09975"
     CDS             complement(4070..5539)
                     /locus_tag="VF547_09975"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8263188.1"
                     /translation="MTGTGRLTVVILGEGVAGWAAAAALAGALPASRYAVSLVPTGMP
                     DDSLGPFGAAEATLPSIREFHARLGLDEEALVRHGRAAFALGVAYSGWSSVAPTYFLP
                     FGDTGAALETVPFHQLAGRLRAAGAEVRFSDFSVAALAAQMGRFARPLDDPASVLSTY
                     SYGLHLPRGGYRDSLRDLALARGVRPHESPFAAAQLSERGDLAGLRLADGETVPGELF
                     IDASGPSGRLIETALTTGFESWRAWLPCDRAVESASSTQIPPAPYSHIEARASGWRRV
                     VPFQGGVGEALVSCSDFADAEGTAFESGRRKRAWNRNCIALGAAAATVEPLQSTSLHL
                     LQTALARLLRLFPDSLDHAAEAAEYNRQTGEELDRLRDFLILRYKLNGRLGEPFWDAV
                     RAMEVPEPLARKIALFESRGRVPLLDGDMFEQEEWAALLDAQGVRPRRYDPRADALPP
                     ERIRAHFTAMRTIMLKAAAALPLHGDYLAREKRVRERVA"
     gene            complement(5536..7053)
                     /locus_tag="VF547_09980"
     CDS             complement(5536..7053)
                     /locus_tag="VF547_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX8263189.1"
                     /translation="MSDNLIRTILIVGGGSSGWMAAAALARKLAGLPIAINLVESAEI
                     GTVGVGEATLPHIRFFNASLGLDEAEFMRRTRATFKLGIEFRNWGRLGDSYIHPFGAF
                     GTDLGGVAFHQHWLRMRSAGEAAPLEDFSLPIQAARMNRFSHPADDPASLYSTFSYAY
                     QFDATLYGRFLRDYAESRGVRRIEGRIVDVEVRPDDGFLSSVTLADGSRLEADLFIDC
                     SGFRGVLIEQALEAGYEDWTRWLPCDRAIALPCAGTGDLVPYTRATARESGWMWRIPL
                     QHRVGNGHVYSSAFLADDDAETALVAQLESPPLAEPNRLRFTTGKRRRQWVRNCVAIG
                     LSAGFLEPLESTSIHLIQLAIGHLIDLLPDRGWDPLDAEEFNRVMALEYERVRDFLIL
                     HYCATERDDTPFWNHCRTMELPDSLSYKMELFRERGYVVNYREGMFLEPSWIAVYLGQ
                     NVTPRRFDPLSETVPESRLRGDMSRIGEACRAAAARMPAHRDFLQAIGAAEGQAA"
     gene            7243..10608
                     /locus_tag="VF547_09985"
     CDS             7243..10608
                     /locus_tag="VF547_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8263190.1"
                     /translation="MEGIESRQLDSARDRFSLVMRGGVSALALCMAAIASPGFAQTAQ
                     PGAEQPSEDDETSTEEQGGVSSGVTDPEAASEGDIVVTGIRRSLQRSQDIKRDSEVVV
                     DSVSAEDISALPDRSVTEALQRIPGVAIDRFAAGRDPDHFSTEGSGVVVRGLTYVRSE
                     LNGRDTFTANNGRGLSFSDVPSELLAGVDVFKSPSADMVEGGISGTVNLRTRVPFDNQ
                     GFVVAASAEGNWGDMREEFTPTFSVLASNRWETGFGEIGLLGSFAYSQLKWRNDAIQI
                     SNFGQRTLYSNGDVVPSTGATAVGTVYLPRGAVMRTQDTDRKRYGYSAAAQWRSNDRS
                     LLATLQFMRSDARESWTEHAIETATDNVTSNGDSRRVPGTTLEFDDDGVFDNGVITGP
                     TGWRDDQQNTAAWGGNGDVRTPRYGLQSNNQKRQQRSRFVTSDYSANLKWNATERLTV
                     NLDYQHVDSRVNQLDVGLWTSAFQNVFIDLDGMRIPTVEFRPPEVCSGPAKNSSCTDL
                     AGGASDQNPSYFGVDPQGGVHNSFSDPYNTFYRNVMDHIEDSDGSEDAARIDLDYSFP
                     ENSWLNSIRVGARHAERENVARFSTYNWGVLSEIWGGRGPVWLTDNIDGNPNTNGGTP
                     ATGAYEAFTYGGFMRGKAGDPLLGQSMLFYSGDPVKDYAGTSAFGLQVGDEWRPRLVD
                     GCPQNWVPLAMRCGVIPGTPFRPGEVNPVDEKNNAAYAMVRFRHEFSSGFKLSGNFGV
                     RYTHTTREAAGTQVFNQVTFSTEAQCLNPTPLPDGSIPAPTPFCALPADVRQAARNFA
                     NGAVATNSAKLSYDYFLPSLNLKAEIGGGVQFRFAYSKGITPPDFGLTRNFYNLTLAA
                     NQQDIEAGGGRPVARSTVGNPYLKPIRSDSFDLSAEYYFNRGGVGQLTLAAFYKRLHG
                     VLVNGTERLPFTNNGATFDAIVTTPVNSTETGKIKGFELAYQQVYSFLPGFLSGLGLN
                     ATYTYVDSSGVSQGTLSETDPDVSAGNTANIDTSLLPLQGLSKHTINIQPFYEKGPLT
                     IRLAYNWRSRFLLTTRDVIVPFAPIFNEPTGQLDGSIFFSLNKRVRIGFQAVNILNEV
                     TKTSQVINNDLIRAPRSWFMNDRRFSGIIRVTM"
     gene            complement(10672..11397)
                     /locus_tag="VF547_09990"
     CDS             complement(10672..11397)
                     /locus_tag="VF547_09990"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEX8263191.1"
                     /translation="MTAASDGYDFSLRPSLALSLERIGLEREPVLIVEGAMNRAGTLV
                     EYAAEEVAFEPVWGPSGGYPGIRAPAPLDYVEAVVRALDPSIRRAFGLDRVKLARAEC
                     SFSLVTLPPDRLAPLQRIPHVDTTDPLQFALLHYLCEPGHGGTAFYRHRATGFETLTP
                     GRQAAYESARAVELEGAPPAYIDADSPHYERTAAFEAAFDRILVYRSRTFHSGRIAAG
                     QALSADPRRGRLTANIFINYRPL"
     gene            complement(11394..13493)
                     /locus_tag="VF547_09995"
     CDS             complement(11394..13493)
                     /locus_tag="VF547_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HEX8263192.1"
                     /translation="MIRTLLFLLVLLIGASPAAGEDGYDLWLRYRPVEPARQNRYAPL
                     LAQLSVPGSSPTLQAARRELARGLDGMLGNRPAAGGVLLAGTPRSSASIARLRLPLAG
                     LGDEGFLIRSVSLDGRPATVIAAHRDIGVLYGAFAFLRLLQTAQPIDALDLREAPRLR
                     LRILDHWDNLDRSVERGYAGESLWDWHRLPGWRDPRYADYARANASIGINGAVLTNVN
                     ADAQALTPLYLRKVAALADDFRSFGIKVYLTARFSAPIELGGLKTADPLDSGVRAWWK
                     AKADEIYRYVPDFGGFLVKANSEGQPGPQDYGRSHADGANMLADALKPHGGIVMWRAF
                     VYTATPATDRAKQAFEEFRPLDGRFRDNVLLQVKNGPIDFQPREPFHPLFGAMPRTPL
                     VMEAQITKEYLGFATHLAYLGTMWEEVLRSDTFARGPGTPVAETLAGMAGVANVGTDR
                     HWSGSHFDQANWYAFGRLAWDPGRPAAAIAEEWARMTWSNDPAVAGTVAAMMMGSRQA
                     VVDYMTPLGLHHLMATGHHYGPGPWVDGLERADWNPTYYHRADRNGIGFDRTASGSNA
                     VAQYAPPVAARFGSLATVPDDYLLWFHHVPWDHRLRSGRTVWDELVWRYSRGVGKVRD
                     MRRAWAALEPRIDRERFAQVAAFLAIQEEEALWWRDASIAYFRSVSRRPLPKGQAAPA
                     HDLDHYKSLRFPHAPGQ"
     gene            13570..14727
                     /locus_tag="VF547_10000"
     CDS             13570..14727
                     /locus_tag="VF547_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX8263193.1"
                     /translation="MKLTRRESLALGAALAVAGCATARGTGGESSSSLDSLARRSGRR
                     FGSAVAWGLPDADRGSFANPAYAAVLERECGLLVPENELKWQWTRPSAAGFDFAQLDS
                     IVEYARRKGFALRGHTLFWAPEKWYPKWLVGHDFGTRPARAAEALLTEHVRTVCRRYG
                     TSIYSYDVVNEAVQPETGAIRDTVVTRAMGGEAMLDLMFHTARAEAPHAELVYNDYMS
                     WERGTEDETHIKGVLKLLEGFRRRGTPVDALGVQSHIRLLKSLPVAEIVRESEGPWRR
                     FLDEVVAMGYKLVITEFDVNDQKAPDDVAVRDRMVADYAKAYLDLMLSYDALRDVLAW
                     GMVDKYSWLTGFDPRPDKSAKRGTPYDSAFRPKPLREAIAAAFAAAPRRRA"
     gene            complement(14786..>15424)
                     /locus_tag="VF547_10005"
     CDS             complement(14786..>15424)
                     /locus_tag="VF547_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8263194.1"
                     /translation="QDFSIVGVVQDLRFRSPREPVRPAMFLLNLAPISAPVATLRATN
                     EVQPVIDELGRRWQKSAAGVPFHATTAESNLLNAYWKLDVQRTRMFGLGAVIALAISS
                     VGLFGLASFASQRRTREMGLRKALGATTPDLARLLLTQFLRPVIIAVVIAWPIAYFAT
                     QQWLAGFDDRIGVGPAYFVAATLAAVALAGVTVLSQVFRLARTRPAEALRHD"
BASE COUNT         2532 a         5170 c         5376 g         2346 t
ORIGIN      
        1 gaaggtcgcg ctgggtcagg atcgggttgc ccggataggc cgaatagggc ccgtcgggcc
       61 ggcggctgcg cagcacgacc tgcgaatggc cctccgccgt cccgccctcg gcggcgagga
      121 gatagtagaa gccgtccttg cgcaggatgt gcgggccctc gatccagatc ggcttcttcg
      181 agagatcgac tccgccgttc accagcacct tgcgcgggcc gaacgtcttc ttcgcggccg
      241 cgtcgaagcg ctggatccag atcgcgcggt gcccctcgta cagcggcggc ccttccggcg
      301 gcccgttgtt gaggatccag acgctgccgt cctcgtcgaa gaagagggag gggtcgatcc
      361 cgccctcgag gtcgggaagc cagaccgggt ccgaccacgg ccccgccggg tcggtcgcgg
      421 tgatgacgaa attgccgccg cagtcgacgc aggtgttgag gatgtagaag gtgccgtcgc
      481 gatgctcgat ggtcggcgcg aagactccgc gcgacaggcc gaggcgggcg aaatcgagct
      541 ggcccggccg gtcgatcgca ttgccgatct gggtccagcc gacgaggtcg cggctgtgga
      601 acaccgggat tcccgggaaa taggagaagg tggaggtgac gagataataa tcgtcgccga
      661 cccgggtcag gctcgggtcc ggatagaagc ccttcaggat cgggttgcga tagtcgccgg
      721 cgccggccgg gagcgagtcg gaaggatcgg cgccgcgata gtcgaagcgt tcgaaccgcg
      781 ccgtcggcgc tccggccggg gcgtccggca cgcttgcgca acccgcgatc agcaaagcga
      841 cggcggccgc cagagcctga tcggctttca tcgaaacgct catccccacg tcatgccagc
      901 gaaagctggc atccatgccg attccgcgcc ccaacgatgc ttcggcatgg accccagcct
      961 tcgctggggt gacggaacgc cagggccctc caggccgatc ctgctgtgga acccccctca
     1021 tgcggcctcc gccagttcgg gcgcggcgca gttctcgagg atgaactgcc tgtgcgtcgg
     1081 catgtactcg gccgaattgt gcaccgtctc ggcgatgtgc cgcagccgcc gccgagtctc
     1141 gtcaagcggc atgatgtcgg ccaccggatc gtaggtccga gccttcatca gctggccgat
     1201 catcaccgcg aaccagctgg tgtcgttgaa cagctcgtca ttctcgcgga aggttcggcc
     1261 ataggcctcg aacacccgga tcttctcggc gagccggtcg gggatcggca tcgtccggca
     1321 ataattccag aattcggaat cgtcccgctc ggtcgccttg tagtggagga cgaggaattc
     1381 gcggacgatc tcatattcgt tgaggacgat gcggttgaac cggtcgatgt ccgcctgctg
     1441 gaaggtccgg tcggggaaca tggtcatcag gcgcgacagc ccgctctgga ccagccagat
     1501 gctggtcgat tcgagcggct cgaggaagcc cgcggcgagc cccagggcga cgcaattctt
     1561 gatccagaac tgcttgcgcc gcccggtctt gaacgggacc acccgggggt cggcgagcgg
     1621 cttgccgtcc agattgccca tcaggacgtc gatcgcctcg tcgtccgtca tgaagtcgct
     1681 cgagaagacg tggccgttgc cgatccggtg ctggagcggg atccgccact gccagccggc
     1741 gctccgggcg gtcgagcggg tgtagggcgt gatctcgggc acgctctcgc acgggacggc
     1801 aatggcccgg ttgcagggca ggtagtgcga ccagtcctcg tagccggcct tgagcgcctg
     1861 ctcgatgatg aggcctcgga agcccgagca gtcgatgaac aggtccgcct cgaccctgtc
     1921 gccattttcc agggtcaccg attcgacgaa gccgtcgtcg ctcctgaggt tgacctcgac
     1981 gatcttgccc tcgcgccgga cgatgccgcg gccctccgcg tagcggcgaa ggtagcgtgc
     2041 atagaggccg gcgtcgaaat gataggcgta ggcgatcgag gagagcggcg agttgccggc
     2101 gtcgaacggc cgcatgaact tgccgcgctc gccggccagc gccggaagcg accattcgtc
     2161 gagccgcggc gcctcgccga gctggtggag ccggagccag tgggcgtgga aggagacgcc
     2221 ctccatgttg agcccatagg agccgaacgg atggaagtag cgatggccct ggcggcccca
     2281 attgtggaac tcgatgccga gcttgaacgt gcccttggtc tcgcggacga aatcgtcctc
     2341 gtcgagctcg agcatgcggt tgaaggtcgc catctgcggg atcgtcgcct cgccgacgcc
     2401 gatgattccg atctcgtccg attcgatcag agtcacgtcg caatatcgag tcgtcaggat
     2461 cttggcgaag gtggccgcgg ccatccagcc ggcggtgccg cctccgacga tgacgacgct
     2521 tcggatacgt tggtcggtca cgccatggct cctcgggatt tcgcggcatc gccgccggcg
     2581 gcgcaatgcc gctttatgta ggcggaatgg ggcggcatgg cttcggccgc cttgcggacg
     2641 aacggcgcca tccgggccag agtcgcctcg atcccggccg aatcgacggt gtcggcgaga
     2701 ggatcgtagc ggcggggccg gacgccctgg ccgagcagga ccgcgagcca gctcgcctcg
     2761 gagaacagtt cgtcctcgtg gcggaagatg cgcccgttct cgcggaacag ctcgatcttg
     2821 cggctcaggc tttcgggcac cgccatggcg cggcagccgc gccagaagtc cgaatcgtcg
     2881 cgctcggtgg ccttgtagtg gaggatgatg aagtcgcgga tctgctcgag ctgcaggtcg
     2941 ctgagccgat tgtattcggc ggcgaggatc gggtcgaagt cgcgatcggg gaagagcgcg
     3001 agcagcttgg agatcccggc ctggacgaga tggatgctgg tcgattcgag cggctcgagg
     3061 aagcctccgg cgagtccgag agcgacgacg ttccggttcc acagcttgcg ccggcggccg
     3121 gccgcgaagc gcaggaagcg cggctcgccg agcgcagccc cggccgcgtt ggcgatcgcc
     3181 gttgcagcgg cctcgtcgtc ggacagatgg gcgctcgaat agacatagcc gttgccggtg
     3241 cggtgctgga gggggatccg ccactgccag cccgcggcga gcgccgtgga gcgggtgtag
     3301 ggtggaggct cggtggcggc cgtgctcgga atggcgacgg cgcggtcgca ggggagccat
     3361 cgcgtccact cctcatagcc cgtctcaagc gcctgctcga tcaggaggcc ccggaaaccg
     3421 gaacagtcga tgaagagatc gccctcgagg cgaagcccgc cggcgagctt caccgcggcg
     3481 acgaagccgt cctcgccgcg cagctcgacg tccaccacct tgccctcgcg gcgaacgacg
     3541 ccccgggcct cggcataggc ccgcagatag gcggcgtaga ggcctgcgtc gaaatggaag
     3601 gcgtagttga gcgtcgacag gatcgagcgc gggtccgcca ccggccgctg gaaccggccg
     3661 agcttcgccg cgacggcgca cagattgtag tcctcgagcc cggccgcccg gccgcgggcc
     3721 tggcacttga gccagaattg gtggaatttc accgcctcga tgtccgcccc gaactgcccg
     3781 aacgggtgca tgtagcgatg gccgggccgc gcccagtcga cgaactcgat cccgagcttg
     3841 aacgtgccct gcgtcttcgc gacgaaatcg tcctcgtcga gcccgagcat ggcgttgaag
     3901 gtgcggatcg gcgggatggt cgcctcgccg acgccgatcg tgccgatctc gtccgattcg
     3961 accagggtga tcgccgcgcc cgccgcccgc agccgcgcca tcgcggccgc ggccatccac
     4021 ccggccgtgc cgccgccgac gatgacaatg tcgcgaatgg gccggccgct catgccaccc
     4081 gctcccgaac ccgcttctcc cgagcgagat agtcgccgtg gagcggaagc gccgcggcgg
     4141 ccttcagcat gatcgttcgc atggcggtga aatgcgcccg gatccgctcg ggcggcaaag
     4201 cgtctgcgcg cggatcgtag cggcgggggc ggacgccctg ggcgtcgagc agagcggccc
     4261 actcctcttg ctcgaacatg tcgccgtcga gcagggggac gcgcccgcgg ctctcgaaga
     4321 gggcgatctt gcgggccagc ggttcgggaa cctccatcgc ccggacggcg tcccagaagg
     4381 gctcgcccag gcggccgttc agcttgtagc ggaggatcag gaagtcgcgg agccggtcca
     4441 gctcctctcc cgtctggcga ttatattcgg ccgcctcggc ggcgtggtcg agcgagtccg
     4501 ggaagagccg caacagcctc gcgagcgccg tctgaaggag atggagcgag gtcgactgaa
     4561 gcggctcgac ggtcgcggcc gcggctccga gcgcgatgca attgcggttc caggcccgct
     4621 tcctgcgccc ggattcgaag gcggtccctt ccgcatccgc gaagtccgag caggagacca
     4681 gcgcctcgcc gacgccgccc tggaacggca ccacccgccg ccaccccgag gcccgggctt
     4741 cgatgtggga atagggggcg ggcgggatct gcgtggaaga ggcggattcg acggcgcggt
     4801 cgcagggaag ccaggcgcgc cagctctcga atccggtggt gagcgcggtc tcgatgagcc
     4861 gcccggaagg accggaggca tcgatgaaaa gctcgcccgg gacggtctcg ccgtcggcga
     4921 gccgaagccc ggccagatcg ccccgctcgc tcagctgcgc ggctgcgaac gggctttcgt
     4981 gcgggcgcac gccgcgggcg agcgcgaggt cccgaagcga gtcgcggtag ccgccgcggg
     5041 gaaggtgcag cccgtagctg taggtcgaga gaacggaggc gggatcgtcc agcggccgag
     5101 cgaagcgccc catctgcgcg gccagcgcgg cgaccgagaa gtcgctgaag cggacctcgg
     5161 cgccggccgc gcgcaggcgc cccgccagct ggtggaacgg gaccgtctcc agggcggcgc
     5221 cggtgtcgcc gaacggcagg aaataggtcg gcgcgaccga cgaccagccg gaataggcga
     5281 ctcccagcgc gaaggccgcc cggccgtggc ggacgagggc ttcctcgtcg agaccgagcc
     5341 gggcgtggaa ctcccggatc gagggaaggg tcgcctcggc ggcaccgaag gggccgaggc
     5401 tgtcgtcggg cattccggtc ggcacgaggc tgacggcgta gcgtgacgcg gggagggcgc
     5461 cggcgagcgc cgcggccgcg gcccagcccg cgactccttc gcccaggatg acgacggtca
     5521 gccggccggt cccggtcatg ccgcttgccc ctccgccgcc ccgatggcct ggaggaaatc
     5581 ccggtgcgct ggcattcgcg ccgccgccgc ccggcaggcc tcgccgatgc gggacatgtc
     5641 gcctctgagc ctggactcgg ggacggtctc gctcagcggg tcgaagcggc gcggagtgac
     5701 gttctggccg agatagaccg cgatccagct cggctcgagg aacatgccct cgcgatagtt
     5761 cacgacgtag ccgcgctcgc ggaacagctc catcttgtag ctcagcgaat cgggcagctc
     5821 catggttcgg cagtgattcc agaagggcgt gtcgtcccgt tccgtcgcgc aataatggag
     5881 gatcaggaag tcgcgcaccc gctcgtattc gagtgccatc acccggttga actcttccgc
     5941 gtcgagcggg tcccagccgc ggtccgggag gaggtcgatg agatggccga tcgccagctg
     6001 gatgaggtgg atgctggtcg attcgagcgg ttcgaggaag ccggcggaca ggccgatcgc
     6061 gacgcagttc cgaacccatt ggcgccgccg cttgccggtc gtgaagcgaa gccgattcgg
     6121 ctcggcgagc ggggggctct cgagctgggc gacgagcgcc gtctcggcgt cgtcgtctgc
     6181 gaggaaggcg ctcgaataga cgtggccgtt gccgacccgg tgctgcagcg gaatgcgcca
     6241 catccagccc gattcgcggg cggtcgcgcg ggtgtagggg acgaggtcgc ccgtgccggc
     6301 gcagggaagg gcgatggcgc ggtcgcaggg gagccagcgg gtccagtcct cgtagccggc
     6361 ctccagcgcc tgctcgatga gaaccccccg gaagccggag cagtcgatga acaggtcggc
     6421 ctccagccga ctgccgtcgg cgagcgtgac cgacgagagg aagccgtcgt ccggccggac
     6481 ctcgacgtcc acgatcctgc cctcgatccg gcggacgccc cgcgattccg catagtcgcg
     6541 aaggaagcgg ccgtagagcg tggcgtcgaa ctgataggcg taggagaaag tggaatagag
     6601 ggaggccggg tcgtcggccg ggtggctgaa gcggttcatc cgggccgcct ggatcggcag
     6661 cgagaagtcc tccagcggcg ccgcctcgcc ggccgagcgc atgcggagcc aatgctggtg
     6721 gaaggcgact ccgcccaggt cggttccgaa ggcgccgaac gggtggatgt aggaatcgcc
     6781 gagtcgcccc cagttcctga attcgatgcc gagcttgaac gtggccctgg tccgtcgcat
     6841 gaactcggcc tcgtcgaggc ccaggctggc gttgaagaag cggatgtgcg gaagggtggc
     6901 ttcgccgacg cccacggtgc cgatctccgc cgattcgacg agatttatcg cgatgggcag
     6961 gcccgccagc ttcctcgcga gcgccgccgc cgccatccag ccggacgagc cgccgccgac
     7021 gatcaggatg gttcggatga ggttgtcgga catcgctacg atccgtccgc gggcccggcg
     7081 cggccctcca ggtcgggaat atggtagttt tgcaacaacc ctgaaactct cttcaccgcc
     7141 ccgccgaggc ccccttgatt gattgaaaaa ccctcgatag ttaccgctaa caacgattag
     7201 cgcggcgtca actgttagcg ctaacatttt ttgggaggtg tcatggaagg gatcgaatcc
     7261 cggcagctcg acagcgctcg cgaccgtttc agcctggtga tgcggggcgg ggtgtcggcc
     7321 ctggcgctgt gcatggcggc gatcgcaagc cccggctttg cccagacggc gcaacccggc
     7381 gccgagcagc cgtccgagga cgacgaaacc tcgaccgagg agcagggcgg agtctcgtcc
     7441 ggcgtgaccg atcccgaggc ggcctcggaa ggcgacatcg tcgtcaccgg catccggcgc
     7501 agcctccagc gttcgcagga catcaagcgg gattcggagg tcgtcgtcga ttcggtgtcc
     7561 gccgaggaca tcagcgcgct tcccgaccgg tccgtgaccg aggccctgca gcgcatcccg
     7621 ggcgtcgcca tcgaccgctt cgccgcgggc cgcgatcccg accatttctc gaccgagggc
     7681 agcggcgtcg tcgtccgcgg cctcacctat gtccgaagcg agctcaacgg ccgcgacacc
     7741 ttcaccgcca acaacggccg cgggctcagc ttctccgacg tgccgtcgga gctgctcgcc
     7801 ggagtcgacg tcttcaagag cccgtcggcc gacatggtcg agggggggat ctcgggcacc
     7861 gtcaatcttc gcacccgggt gcccttcgac aaccagggct tcgtcgtcgc cgcttccgcc
     7921 gagggcaatt ggggcgacat gcgcgaggag ttcacgccca ccttctcggt gctcgccagc
     7981 aatcgctggg agaccggatt cggcgagatc ggactgctcg gcagcttcgc ctattcgcag
     8041 ctcaagtggc gcaacgacgc gatccagatc tcgaacttcg gccagcgcac gctctattcg
     8101 aacggcgacg tcgttccgag caccggggcg accgccgtgg gcaccgtcta cctgccgcgc
     8161 ggcgccgtca tgcggaccca ggataccgac cgcaagcgct acggctattc ggccgcggcg
     8221 caatggcgca gcaacgaccg gtcgctgctc gcaacgctcc agttcatgcg ctccgacgcg
     8281 cgcgaatcct ggaccgagca tgcgatcgag accgccacgg acaacgtcac gtccaacggc
     8341 gattcgcggc gcgtgccggg cacgaccctg gaattcgacg acgacggcgt cttcgacaac
     8401 ggggtcatca ccggtccgac cggctggcgc gacgaccagc agaacaccgc cgcctggggc
     8461 gggaacggcg acgtccgcac cccgcgctac ggcctgcaga gcaacaacca gaagcgccag
     8521 cagaggtcgc ggttcgtcac cagcgattac agcgccaatc tgaagtggaa cgcgaccgag
     8581 cggctgaccg tcaacctcga ctatcagcat gtcgattcgc gggtgaacca gctcgacgtc
     8641 gggctctgga cgtcggcctt ccagaacgtc ttcatcgatc tggacggcat gcgcatcccg
     8701 accgtcgagt tccggccgcc ggaagtctgc tcggggcccg cgaagaacag ctcctgcacc
     8761 gacctcgccg ggggcgcttc cgaccagaat cccagctact tcggagtcga cccgcagggc
     8821 ggcgtccaca acagcttctc ggatccgtac aacaccttct accgcaacgt catggaccat
     8881 atcgaggaca gcgacggcag cgaggacgcg gcccggatcg acctcgatta ctccttcccc
     8941 gagaacagct ggctgaactc tatccgggtc ggcgcacgcc acgccgagcg cgagaacgtc
     9001 gcccgcttct cgacctacaa ttggggcgtg ctgagcgaga tctggggcgg ccgaggcccg
     9061 gtctggctga cggacaatat cgacggcaat cccaacacca acggcggcac gcccgcgacc
     9121 ggcgcgtacg aggccttcac ctatggaggc ttcatgcgcg gcaaggccgg ggacccgctg
     9181 ctgggccagt cgatgctctt ctattccggc gatccggtga aggactacgc gggcaccagc
     9241 gccttcggcc ttcaggtggg cgacgaatgg cggccgcggc tggtcgacgg ctgcccgcag
     9301 aactgggtgc cgctggcgat gcgctgcgga gtgatcccag gcacgccgtt ccggcccggc
     9361 gaggtcaatc cggtcgacga gaagaacaac gccgcctacg cgatggttcg gttccgccac
     9421 gaattttcga gcggattcaa gctcagcggc aatttcggcg tccgctacac gcacaccacc
     9481 cgcgaagcgg ccggcaccca ggtgttcaac caggtcactt tctcgaccga ggcccagtgc
     9541 ctcaatccga ccccgctccc cgatggctcg ataccggcgc cgacgccctt ctgcgccttg
     9601 ccggccgacg tccgccaggc ggcgcgcaat ttcgccaacg gcgcggtcgc gaccaactct
     9661 gccaagctca gttacgatta tttcctgccg agcctgaacc tgaaggcgga gatcggcggg
     9721 ggagtccagt tccgcttcgc ttattcgaag ggcatcacgc cacccgattt cgggctgact
     9781 cggaacttct acaacctcac gctcgccgcc aaccagcagg atatcgaggc cggcggcggc
     9841 cgcccggtcg cccgatcgac ggtcggcaac ccctatctga agccgatcag atccgacagc
     9901 ttcgaccttt ccgcggaata ttatttcaac cgcggcggcg tcggccagct gacgctcgcg
     9961 gccttctaca agaggctgca cggcgtgctg gtcaacggga cggagcggct tccgttcacc
    10021 aacaacggcg ccaccttcga cgcgatcgtg acgactccgg tcaactcgac cgagacgggc
    10081 aagatcaagg gcttcgagct ggcctatcag caggtctaca gcttcctgcc cggcttcctc
    10141 tcgggcctcg gcctcaacgc gacctacacc tatgtcgact cgagcggcgt gagccagggc
    10201 accttgtccg aaaccgatcc ggacgtctcg gccggcaaca cggccaatat cgacaccagc
    10261 ctgctgcccc tccagggcct gtccaagcat acgatcaaca tccagccctt ctacgagaag
    10321 gggccgctga ccatccgact cgcctacaac tggcgatcgc gcttcctgct caccacccgc
    10381 gacgtgatcg tgccgttcgc cccgatcttc aacgagccaa ccggccagct cgacggctcg
    10441 atcttcttct cgctcaacaa gcgggtgcgg atcggcttcc aggccgtgaa catcctcaac
    10501 gaggtcacca agacctcgca ggtgatcaac aacgacctca tccgggcgcc gcgctcctgg
    10561 ttcatgaacg accggcggtt ctccggcatc atccgggtca ccatgtagcc gttcgtcccg
    10621 aactcgaggc gccgcgatcc gtcgcggcgc ctttttttgt tttttcggcg atcagagcgg
    10681 gcggtagttg atgaagatgt tggcggtgag ccggccccgc cgaggatcgg cgctcagcgc
    10741 ctggccggcg gcgatccggc ccgaatggaa ggtccggctg cggtagacga ggatccggtc
    10801 gaaggcggcc tcgaacgcgg cggtgcgctc gtaatgcggg ctgtcggcgt cgatataggc
    10861 cggcggcgcc ccctcgagct cgaccgcccg ggccgattcg taggccgctt gccggccggg
    10921 cgtcagcgtc tcgaacccgg tggcgcgatg ccggtagaag gcggtgccgc cgtggcctgg
    10981 ctcgcaaaga taatggagca gcgcgaactg cagcggatcg gtcgtgtcca catgggggat
    11041 gcgctggagc ggcgcgagcc ggtcgggcgg cagggtgacg agcgagaagc tgcattccgc
    11101 ccgcgcgagc ttgacccggt cgagcccgaa cgcccggcgg atgctcggat cgagggcccg
    11161 caccaccgcc tccacatagt cgagcggcgc cggcgcgcgg attccgggat agccgcctga
    11221 aggcccccag accggctcga acgccacctc ctccgccgca tattcgacca gcgtcccggc
    11281 ccggttcatc gccccctcca cgatcagcac cggctcgcgc tccaggccga tccgctcgag
    11341 cgaaagcgcg aggctgggcc gaagcgagaa atcatagccg tccgaggccg cggtcattgg
    11401 ccgggcgcgt gcgggaagcg cagcgacttg taatggtcga ggtcgtgcgc gggggccgcc
    11461 tgacccttgg gcagcggccg gcgcgagacg cttcggaaat aggcgatcga ggcgtcgcgc
    11521 caccagagcg cctcctcctc ctggatggcg aggaaggcgg cgacctgagc gaagcgctcg
    11581 cggtcgatcc gcggctcgag cgcggcccag gcgcggcgca tgtcccgaac cttcccgacg
    11641 ccgcgcgaat agcgccagac gagctcgtcc cagaccgttc gtcccgatct taaccgatgg
    11701 tcccagggca cgtggtggaa ccacaggaga tagtcgtccg gcacggtcgc gaggctgccg
    11761 aagcgggcgg cgacgggggg cgcatattgg gcgacggcgt tgctgccgga ggcggtgcgg
    11821 tcgaagccga tcccgttgcg gtcggcgcgg tgataatagg tcgggttcca gtccgcccgc
    11881 tcgagcccgt ccacccaggg gccagggccg taatggtgcc cggtcgccat aagatggtgg
    11941 agccccagcg gggtcatgta gtcgaccacc gcctggcggg agcccatcat catcgccgcg
    12001 accgtcccgg ccaccgccgg atcgttgctc caggtcatcc tcgcccattc ctcggcaatg
    12061 gcggccgcgg ggcggcccgg gtcccaagcg aggcggccga aggcgtacca attggcctgg
    12121 tcgaaatggg agccgctcca gtggcggtcg gtgcccacat tggccacccc ggccatgccg
    12181 gccagggtct cggcgaccgg ggtgccggga ccgcgggcga aggtgtcgct gcgcaggacc
    12241 tcctcccaca tcgtgccgag ataggcgagg tgggttgcga agccgagata ttccttggtg
    12301 atctgggcct ccatcacgag cggagtcctc ggcatcgcgc cgaacaacgg atggaagggc
    12361 tcgcgcggct ggaagtcgat cggcccgttc ttgacctgga ggagcacgtt gtcgcggaag
    12421 cggccgtcga gcggccgaaa ttcctcgaag gcctgcttgg cccggtcggt ggccggcgtc
    12481 gccgtgtaga cgaaggcccg ccacatgacg atgccgccgt gcggcttcag cgcgtcggcc
    12541 agcatgttgg cgccgtcggc gtggctgcgc ccgtaatcct gcgggccggg ctggccttcc
    12601 gaattggcct tgaccaggaa gccgccgaaa tcggggacgt agcggtagat ctcgtccgcc
    12661 ttggccttcc accaggcgcg cacgccggag tccagcgggt cggcggtctt gaggccaccc
    12721 agctcgatcg gcgcgctgaa gcgggcggtg agatagacct tgatcccgaa cgagcggaag
    12781 tcgtcggcca gcgccgcgac cttccggaga tagagcggcg tcagcgcctg ggcatcggcg
    12841 ttgacgttgg tgaggacggc gccgttgatt ccgatcgagg cgttggcccg cgcataatcg
    12901 gcgtagcgcg gatcccgcca tccgggcagc ctgtgccagt cccacagcga ctcgccggcg
    12961 tagccgcgct cgacgctgcg atcgagattg tcccaatggt cgaggatccg gagcctgagc
    13021 ctgggcgcct cgcgaaggtc gagcgcgtcg atcggctgcg cggtctggag aaggcgcagg
    13081 aacgcgaagg cgccgtagag gacgccgatg tcgcgatggg cggcgatgac cgtcgccggt
    13141 cgtccgtcga ggctgaccga gcggatcagg aatccttcgt cgccgaggcc ggccagcggc
    13201 agccgcaggc gggcgatcga agccgagctg cgcggcgttc cggcgagcag gacgccgccc
    13261 gcggcgggcc tgttcccgag catcccgtcg aggcctcgcg ccagctcgcg ccgggccgcc
    13321 tgaagcgtcg gcgacgagcc cggcaccgag agctgcgcga gaagcggcgc gtaccgattc
    13381 tgccgggccg gctcgaccgg acggtagcga agccacagat catagccgtc ctcccccgcc
    13441 gcggggctcg cgccgatcag caggacgagc aggaacagca gggttcggat catggtttcc
    13501 taaccgccgg atttgacgcg ggcagatagc atgttaacgc taccagtcaa agctggcagg
    13561 agaggtccga tgaagctcac ccggcgcgag agcctggccc ttggagccgc cctggcagtc
    13621 gccggctgcg cgaccgctcg cgggacgggg ggcgaatcct cctcctcgct cgactcgctc
    13681 gcccggcgta gcggccgcag gttcggctcg gcggtcgcct gggggcttcc cgacgccgac
    13741 cgcggcagct tcgccaatcc ggcctatgcg gccgtgcttg agcgggagtg cgggctgctc
    13801 gtccccgaga acgagctcaa gtggcaatgg acgcgcccgt cggcggccgg cttcgacttt
    13861 gcgcagctcg actcgatcgt cgaatatgcc cggaggaagg gtttcgcgct gcgcggccac
    13921 acgctctttt gggcgccgga gaaatggtat cccaaatggc tcgtcggcca tgatttcggg
    13981 acccggccgg cccgggcggc ggaggcgctg ctgaccgagc acgtccgtac cgtctgccga
    14041 cgctacggga cgagcatcta ttcgtacgac gtcgtcaacg aggccgtgca gcccgagacg
    14101 ggcgcgatcc gcgacacggt ggtcacccgc gcgatgggcg gcgaggcgat gctcgacctg
    14161 atgttccaca ccgcccgcgc cgaggcgccc catgccgagc tcgtctacaa cgattatatg
    14221 agctgggagc gcggcaccga ggacgagacc cacatcaagg gcgtgctcaa gctcctcgag
    14281 ggcttccgcc ggcgcgggac gccggtcgac gcgctcggcg tgcagtcgca catccgcctg
    14341 ctgaagagcc tgccggtcgc ggagatcgtc cgcgaatccg aagggccctg gcgccgcttt
    14401 ctcgatgagg tggtggccat gggctacaag ctcgtcatca ccgagttcga cgtcaacgac
    14461 cagaaggcgc cggacgacgt cgcggtgcgg gaccggatgg tcgcggatta tgcgaaggcc
    14521 tatctcgacc tgatgctgag ctacgacgcg ctccgcgacg tcctcgcctg ggggatggtc
    14581 gacaagtaca gctggctcac cggcttcgat ccgcggccgg acaagtcggc caagcgcggg
    14641 acgccctacg attccgcctt tcggcccaag ccgcttcgcg aggcgatcgc cgccgccttc
    14701 gcggccgcgc cgcgccgccg cgcttgaacg gcagggcgct ccggtccgag tgcctcaagt
    14761 cgatcggatc ggtccgcgcc tcggctcagt cgtgccgcag cgcttccgcc gggcgggtcc
    14821 gggccaatct gaaaacctgt gaaagcacgg tgacacccgc cagcgccact gcagcgaggg
    14881 tcgccgcgac gaaataggcg gggccgacgc cgatgcggtc gtcgaagccg gcgagccact
    14941 gctgggtcgc gaaataggcg atcggccatg cgatcacgac ggcgatgatc accggccgca
    15001 ggaactgggt cagcagcagc cgcgcgagat ccggcgtggt ggcccccagc gccttgcgca
    15061 gccccatctc gcgggtgcgc cgctgcgacg cgaacgaggc gaggccgaac agccccacgc
    15121 tgctgatcgc gagcgcgatg acggcgccga gcccgaacat ccgggtccgc tggacgtcga
    15181 gcttccaata ggcgttgagg aggttcgatt ccgccgtcgt cgcgtggaaa ggcactccgg
    15241 cggcgctttt ctgccagcgc cgtccgagct cgtcgatgac gggctgcacc tcgttggttg
    15301 cgcgcagggt ggccacgggc gcgctgatcg gagcgagatt gagcaggaac atcgccggcc
    15361 gcaccggctc gcgcggggag cggaagcgga gatcctgcac gacgcccacg atggagaagt
    15421 cctg
//
LOCUS       DASVQZ010000351         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_73328, whole genome shotgun sequence.
ACCESSION   DASVQZ010000351 DASVQZ010000000
VERSION     DASVQZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73328"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF547_10010"
     CDS             <1..203
                     /locus_tag="VF547_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263195.1"
                     /translation="PAAGVLVLRLDGGGAALFGLLVEETHLVHGAGKAPRWARFPLFI
                     VPIAPDRPHPGRRFLTYVYSSF"
     gene            331..618
                     /locus_tag="VF547_10015"
     CDS             331..618
                     /locus_tag="VF547_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263196.1"
                     /translation="MKKLIIAAMAGIVATPAIAQGGTVLGAPQFDSYANRGQCESALA
                     AERNKQRKDPATRGAGYQDLTGSEFNKASRSTTRCEERGGKFVVVYYQNGF"
     gene            complement(677..1072)
                     /locus_tag="VF547_10020"
     CDS             complement(677..1072)
                     /locus_tag="VF547_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263197.1"
                     /translation="MARRVKEAAAAALILAGLSAPAPAQRAGGAVTAARADWADMAGG
                     TYFGDLTSDARGTPRKRVSLAVAKIGPNQVRVTSDHPRLPAFTVRLIRAPDILRNADG
                     PVVFLLDLSKSPRTLTVTVDGASWTGSRQ"
     gene            complement(1114..1542)
                     /locus_tag="VF547_10025"
     CDS             complement(1114..1542)
                     /locus_tag="VF547_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263198.1"
                     /translation="MRKLAMVLALLMLGEAALAAADPAAWVKRILAEYDSIQIKQGWA
                     PQGEARPGALASGKEARLDFALAPGTYRFVVVCESACGAGEVELLDPAGARVGGPGVL
                     VEMPKIDLSPTAAGPYVLRVAMTDCASPQCAWSARLYAKR"
     gene            complement(1611..2132)
                     /locus_tag="VF547_10030"
     CDS             complement(1611..2132)
                     /locus_tag="VF547_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HEX8263199.1"
                     /translation="MADLPPWHRIAVEELAASVAERADGEHPRILEYLGTCSDLEEGE
                     WDRDSTPWCSAFVNWCLARSGIEGTDSGWARSWAEWGRPIEEARLGAIAVWARGRTRA
                     DEPVVTGHVAFVVEDLGDSLLVLGGNQSDRVCLKTYPKRGYLTDTVASATPVRELYEL
                     IGYRWPAGVPDDG"
     gene            complement(2239..3168)
                     /locus_tag="VF547_10035"
     CDS             complement(2239..3168)
                     /locus_tag="VF547_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263200.1"
                     /translation="MRATRFLLGLGIAALVPGNPAIAVDCTLKSGVTAAQGREGLCGF
                     SAANRSFRGTPAQQAECLTRAVGRGAAIGAPTLTPFLRERVGQPTNVTRARLAAYLAE
                     LHVDPGRLGGPLADPVSARYFIIHDTSTPNCSAADFSTQLCPARGIMPPNRDTAEWAA
                     IAKFFGHPKPFPNRLAHAFTNRVGDSITEVPFDAHISTTKFEGCSDSAAKQRLFIGIE
                     NIQPRIGAPAVPAPGKKANDLVAPVPGFTTAQYRRLALLYVTASVREGRWLIPAFHAV
                     LDSRYADGHDDPQNFDVPAFSDAVRAHLDKLAS"
     gene            complement(3368..>3727)
                     /locus_tag="VF547_10040"
     CDS             complement(3368..>3727)
                     /locus_tag="VF547_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX8263201.1"
                     /translation="VIATPDMMGVVGRLGKVLGPKGLMPNPKLGTVTPNVTEAVKAAK
                     GGQVEFRVEKAGIIHSGIGKASFPAEDLKRNFTAFVDAIVKAKPAGAKGKYVKKVALS
                     STMGPGLKVDVAEVASA"
BASE COUNT          573 a         1333 c         1260 g          561 t
ORIGIN      
        1 agcccgcggc gggcgtgctc gttctgcgcc tcgacggcgg cggcgcggca cttttcggcc
       61 tgctggtgga agaaacgcac ctcgtccatg gcgcaggaaa agccccgcgc tgggcaaggt
      121 tcccgctttt tatcgtacca atcgcaccgg atcgcccgca cccgggccgc cgatttctaa
      181 cctatgttta ttcaagcttc tgataaataa tggaaatctg acgagacgcg cggaatcccc
      241 gccgaagcga ggggccggcg cgcccgccgg ccgttctacc ggcgcgcgtc gcggccgatg
      301 ccgccgcgag cgcccaaagg agtattggaa atgaaaaagc tgatcatcgc tgccatggcg
      361 ggcatcgtcg ccacgccggc gatcgcccag ggcgggaccg tcctcggcgc gccgcaattc
      421 gattcctatg ccaaccgggg ccagtgcgag agcgcgctgg cggccgagcg caacaagcag
      481 cgcaaggacc ccgccacccg cggcgccggc tatcaggacc tcaccggttc cgaattcaac
      541 aaggcgagcc ggtcgacgac ccggtgcgag gagcgcggcg gcaagttcgt cgtcgtctac
      601 taccagaacg gcttctagac gcgtcgccgg ccgacgacgg ggccggggcg aaagctccgg
      661 ccctcctccc ttgagcctat tgcctcgatc cggtccagga cgcgccgtcg accgtgacgg
      721 tcaacgtccg cgggcttttc gacaggtcga gcaggaagac caccggaccg tccgcattcc
      781 gcaggatgtc cggggctcgg atcagcctga ccgtgaaggc cggcagccta ggatgatcgg
      841 acgtcacccg gacctggttc gggccgatct tggcgacggc gaggctcact cgcttgcgcg
      901 gcgttccccg ggcgtcggag gtcaggtcgc cgaaataggt gccgccggcc atatcggccc
      961 agtccgcccg cgccgcggtg accgcgccgc cggctcgttg cgccggggcc ggcgccgaga
     1021 ggcccgcgag gatcagcgcc gccgcggcgg cctccttcac tcgtcgagcc atgcctgcct
     1081 cctccaccct tcaggcctcc gccgccagca cgctcacctc tttgcgtaga gccgcgccga
     1141 ccaggcgcat tgcggcgagg cgcagtcggt catcgccacg cgcaggacat acggccccgc
     1201 cgccgtcggc gacaggtcga tcttgggcat ctcgaccagc acgccgggcc cgccgacccg
     1261 ggcgccggcc gggtcgagaa gctccacctc gccggctccg caggcggatt cgcagaccac
     1321 gacgaatcgg taggttcccg gcgcaagcgc gaaatcgagc cgggcctcct tgccgctcgc
     1381 aagagcgccc ggccgcgcct cgccctgcgg ggcccagccc tgcttgatct ggatcgaatc
     1441 atattcggcg aggatccgct tcacccaggc cgcaggatcg gccgccgcca acgctgcctc
     1501 ccctaacatg agcagcgcca ggaccatcgc cagtttccgc atctcagtct ccgccggtgc
     1561 cgttcgcgcc ggcatgcccg attcgaacgg cggggcaaag gccggcgcgc tcagccatcg
     1621 tcgggcaccc ctgccggcca tcggtagccg atcagctcgt acagctcgcg caccggagtc
     1681 gcgctcgcca ccgtgtcggt gagatagccg cgcttgggat aggtcttcag gcacacccga
     1741 tcggactggt tgccgccgag cacgaggagg ctgtcgccga gatcctcgac cacgaaggcg
     1801 acatgtccgg tgaccaccgg ctcgtccgct cgggtccgcc cccgcgccca gaccgcgatc
     1861 gcgcccagcc gcgcctcttc gatcggccgc ccccattcgg cccacgaccg cgcccagccc
     1921 gaatccgtcc cctcgattcc gctccgcgcc aggcaccagt tgacgaaggc cgagcaccag
     1981 ggcgtcgaat cgcggtccca ctccccttcc tccaggtccg agcaggtgcc gagatactcg
     2041 aggatccgcg gatgctcgcc gtccgcccgc tcggcgacgc tcgcggcgag ctcctcgacc
     2101 gcgatccggt gccagggggg aaggtcggcc atggcgggcc cgtagcgctt ccgtcccccg
     2161 ggcacaaaag ggatcgaatg cccgtgccta cgaaaatgcg tttgcgcgcc cgcagcgggc
     2221 aaacgggctc ggaaaggcct aggaggccag cttgtcgaga tgcgccctca ccgcgtcgga
     2281 gaaggccggc acgtcgaaat tctgcgggtc gtcatggccg tcggcatagc ggctgtcgag
     2341 caccgcgtgg aaggccggga tgagccagcg gccctcgcgc acgctcgccg tcacgtagag
     2401 caacgccagc cgccggtatt gagcggtcgt gaagccgggg acgggcgcga ccagatcatt
     2461 ggccttcttg cccggcgcgg ggaccgccgg agcgccgatc cggggctgga tgttctcgat
     2521 gccgatgaac agccgctgct tggcggccga gtccgaacag ccctcgaact tggtcgtcga
     2581 gatgtgggca tcgaatggca cttcggtgat cgaatcgccg acacggttgg tgaaggcatg
     2641 ggcgaggcgg ttcggaaaag gcttggggtg gccgaagaac ttcgcgatcg ccgcccattc
     2701 ggccgtgtcg cggttgggcg gcatgattcc gcgcgccggg cagagctggg tgctgaaatc
     2761 cgccgcggag caattggggg tgctggtgtc gtgaatgatg aagtagcgcg cggacaccgg
     2821 atcggcgagc gggccgccga ggcggccagg atcgacgtgg agctccgcca gataggccgc
     2881 cagccgcgcg cgggtgacgt ttgtcggctg tccaaccctt tcccgaagga acggggtcag
     2941 ggtcggcgcg ccgatcgcgg cgccccgccc gaccgcccga gtcaggcatt cggcctgctg
     3001 ggcgggagtg ccccggaacg accggttcgc cgcgctgaat ccgcacaggc cctcgcgccc
     3061 ctgcgcggcg gtgaccccgc tcttcagcgt gcagtccacg gcgatcgccg gattgcccgg
     3121 gacaagcgcc gcgatgccga gtcccaacag aaagcgcgtc gcccgcatcg cctggtccct
     3181 tccgaagccg cgccggggcg cgtcgtgagt gccccggtcc gcgccggaga ctagccagat
     3241 tgcggcagcg cgggcgtgat ggcaatcacg gggatcgggg ccggcgtcca aaaggaaagg
     3301 gccggaagct ttcgccccgg cccttccgca ttccgtcgcg aacgaagcct cgcttcgtcc
     3361 cggttcctta cgcgctcgcc acctcggcga cgtcgacctt gaggcccggc cccatggtcg
     3421 agctcagcgc gaccttcttg acgtacttgc ccttggcgcc ggccggcttg gccttgacga
     3481 tggcgtcgac gaacgcggtg aagttgcgct tgaggtcctc ggccgggaag ctcgccttgc
     3541 cgatcccgct gtggatgatc ccggccttct cgacccggaa ctcgacctgg ccgcccttgg
     3601 ccgccttgac cgcctcggtg acgttggggg tgacggtgcc gagcttcggg ttcggcatca
     3661 gccccttggg gccgagcacc ttgccgaggc gtccgaccac acccatcatg tcgggcgtgg
     3721 cgatgac
//
LOCUS       DASVQZ010000352         8881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_844036, whole genome shotgun sequence.
ACCESSION   DASVQZ010000352 DASVQZ010000000
VERSION     DASVQZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844036"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VF547_10045"
     CDS             complement(<1..199)
                     /locus_tag="VF547_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263202.1"
                     /translation="MKKSATEVMQEIVAAAVLDSIAALKAASRGLPNNLLRDLNAVHA
                     NTTFADLPAEVQGAITASVRAA"
     gene            290..1030
                     /locus_tag="VF547_10050"
     CDS             290..1030
                     /locus_tag="VF547_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8263203.1"
                     /translation="MPTKPLTLALLAGLALAGAGCATRPAAPTVDYAAILADPVRPEA
                     DRARDADRKPAELVAFAGVRPGDKVAELAPGGGYFTRILSGVVGPEGRIYAMAGRPSP
                     ALQELAAARPNVSVITVEPGTVPAPEPVDLVWTTLNYHDFKNAKVGEGDGAAAINAAA
                     FRALKPGGVYLVVDHDTAPGAGASQTSTLHRIESALVRREVESAGFRLEAESSLLRHP
                     EDDHSARVQETGIRGKTDQFVLRFRKPR"
     gene            1220..1444
                     /locus_tag="VF547_10055"
     CDS             1220..1444
                     /locus_tag="VF547_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263204.1"
                     /translation="MIGIALMAAAAHAPVLLAANDEAQWRRFVRASLQRPAAPAARAP
                     SRLNQLQGVVRRLDDDRSGPASASAGPYGL"
     gene            complement(1481..3280)
                     /gene="phaC"
                     /locus_tag="VF547_10060"
     CDS             complement(1481..3280)
                     /gene="phaC"
                     /locus_tag="VF547_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX8263205.1"
                     /translation="MSDPPSTVFKLPESLPSPADLAEAWSKVVANAVHLATSAAARPL
                     DAHVPQPFDAAAPARAFGEFAAHLWSHPQELVKAQQKAASDWMKLWQSAAARAAGRAA
                     DPVAVPERGDRRFNDPAWSDEPVFDYLKQAYLLTAKRALEMVEEAEGLDESARTRVEF
                     FTQQYLNAISPANFAFTNPEAIRKAIDTGSISLLSGLANLLADAAQGGLVQRRAEREF
                     ELGVDIAATPGSVVYRNALMELIQYAPATDQVYRRPILYVPPLVNKYYLLDLQPKSSL
                     MRWLVEQGHTVFVISWVNPGPELADKAIADYIRQGPLEALDAIEAATGEREVDLFGFC
                     MGGTLAAIALAWLAAKGEGGRVASATTIGTMFDFSRLGQWATFSEPEQLRAMERHLLH
                     KGFMAAQDLQALFSAVRANDLIWSSVVNHYLLDREAPPSDILHWFADGAHIPRAFLLE
                     WAKDILKDNKLAKGGLELDGVRIELGAVKTPLLAISLKDDHVSAWQATYDSARLFGGD
                     VAFLLGGSGHNAGVINPPSANKHGYWVNAAMPETAEQWLEGAERREGSWWPWWQARLS
                     EDGKAEKVKARAPGDGKLKALEPAPGSYVRNRA"
     gene            complement(3422..3610)
                     /locus_tag="VF547_10065"
     CDS             complement(3422..3610)
                     /locus_tag="VF547_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263206.1"
                     /translation="MFEARTAAVLANHQAPASVAAADFRTPADPEAGWAARNPDRAML
                     FLFVSPRDCGALEELMAL"
     gene            3711..4607
                     /locus_tag="VF547_10070"
     CDS             3711..4607
                     /locus_tag="VF547_10070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8263207.1"
                     /translation="MAEFDWNDVRAFLAVARSGRLTAAAGRLGVDHSTLSRRIAALEH
                     ALRSKLFDRSPSGYTLTEEGRRLLPIGEEMERLAIGAGEAIGGTSTSVAGTVRIGSPE
                     GFGSYFLAPRIAGLKARHPRLTIQLVAAPAVFSLARRDADVVISVSRPPAGRLHVSKL
                     IDYDLGLYAAPGYLRQAPDIASAGDLSRHRFVSYIGELLHFPELDFLQHVAPGGTTSV
                     ESSNLVAQLRATLAGAGLCVLPAFLAGEEDGLVRVLPGEINLTRSLWLTVHQDLAELA
                     RVRAAVAFIREEVESARGLFKL"
     gene            complement(4604..5368)
                     /locus_tag="VF547_10075"
     CDS             complement(4604..5368)
                     /locus_tag="VF547_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8263208.1"
                     /translation="MFDLGGVGLLDILPFIAIGFAAQLIDGALGMAFGQISSTLLISM
                     GVPPAAASAGVHTAETFTTAVSGVSHVLHRNVDWRLFFRLAVPGVIGGVIGAYVLSNI
                     DASTAKPIVLAYLAALGLYLLYRGVMHRHNEREPRIVAPLGLAGGFLDAAGGGGWGAV
                     VTSNLLVQGSSPRKTIGTVNTAEFFLTVTISATFIATLGWEAFTVATLGLLIGGVMAA
                     PFGAWIAKKVDADRLLTFVGIVLTASSSFGLYRALA"
     gene            5520..6215
                     /gene="epsC"
                     /locus_tag="VF547_10080"
     CDS             5520..6215
                     /gene="epsC"
                     /locus_tag="VF547_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX8263209.1"
                     /translation="MLPGLVAYLDSVKARDPAPRSRWEVLLYPGVWALGFHRLAHWLY
                     SGELYFLARLVNHLGRFLTAIDIHPGASVGRNFFIDHGFVVIGETATIGDDVTIYQQV
                     TLGGTNPTDGIPGKRHPSIGNHVIIGSGAQVLGPIIVGERARIGANAVVTKDVPEGAT
                     MTGIPARSTLLSAEQSSSGNFLPYGTPCSEQCDPAAQKLEALQHEVEQLRRRVADLIE
                     ERDGKTRKERGRA"
     gene            6215..6553
                     /locus_tag="VF547_10085"
     CDS             6215..6553
                     /locus_tag="VF547_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX8263210.1"
                     /translation="MGTVHPFPGASSPQIGFTRSELKRIIDLYGRMVAAGLWRDYAVE
                     FGRDGAAFWAFRRTAERPEYKIEKRPALRNRQGLWALVNEAGMVIRRGDELGPVLAPL
                     ERRLLKVVNG"
     gene            complement(6694..7785)
                     /locus_tag="VF547_10090"
     CDS             complement(6694..7785)
                     /locus_tag="VF547_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263211.1"
                     /translation="MYSDSDLDAAVAAGALSPESASALRAYMDSRATSSPVDEEHFRL
                     ITGFNDIFVSIAAAILLFAVGWIGNSMGANIDGDGPSPFAGLLVAGTAWGLAEFFTKK
                     RRMALPSILLLLAFTGGVLLTTFSALVLALGEATFENNERAVALVIAGSAAVAAAATW
                     MHWRRFRVPITVAAGALTVVGVVMALIASAVGDVPALEDIMLASGLVLGLGVFLFAMW
                     WDSSDRARQTRRSDVAFWLHLLAAPMIVHPIFSLLGLTDGDASIGEALVVLGVYVLLG
                     ITALAIDRRALLVSALAYVLFALISLFREFGAVELNIALAAFVIGSGLLLLSAFWHSV
                     RRSIVRPLPPGLQERLPVLDRAAPAAAAA"
     gene            complement(7957..8856)
                     /locus_tag="VF547_10095"
     CDS             complement(7957..8856)
                     /locus_tag="VF547_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8263212.1"
                     /translation="MTISFEGRVAIVTGAGGGLGRAYALELARRGARVVVNDLGGARD
                     GSGRSDAAQRVVEEIEAAGGEAIANGASVTDSAQVEAMVAAAKERWGGVHILINNAGI
                     LRDKSFAKMSVEDFRAVVDVHLIGSAVCTKAVWETMREQAYGRILMTSSSTGLYGNFG
                     QANYGAAKMGLAGLARTLHLEGAKYDIRVNTIAPTAATRMTEDLFPPEMLSLFNPQNV
                     VPAALYLVSEEAPSGAIVGAGGGVVQAAYVTLTPGYALPEGRRTVEELAANWDKVTDR
                     TLEVVPGSGAEQAMTILKTLQGN"
BASE COUNT         1381 a         3074 c         3074 g         1352 t
ORIGIN      
        1 aggcggcgcg aacgctggcc gtgatggccc cctgcacctc ggccggcagg tccgcgaagg
       61 tggtgttggc atggaccgcg ttcagatcgc gaagcaaatt gttgggcagc ccccgcgacg
      121 ccgccttcag agccgcgatc gagtcgagca cggccgcggc gacgatttcc tgcatgacct
      181 cggtggcgga tttcttcatt cggccactct taggggagaa agcggacgct cactagtgac
      241 tgtccctccc ccggtgcata agggccaccg aacacgacag gaggtccgga tgccgaccaa
      301 gccgctcact ctcgccctgc tcgccggcct cgccctcgcc ggcgccggct gcgcgacccg
      361 gccggccgcg ccgaccgtcg attatgcggc gatcctcgcc gatccggtcc gccccgaggc
      421 cgaccgggcg cgcgacgcgg accgcaaacc cgccgagctg gtcgccttcg ccggcgtcag
      481 gcccggcgac aaggtcgccg agctcgcgcc cggcggcggc tatttcaccc gcatcctgag
      541 cggcgtcgtc gggccggagg ggcggatcta cgccatggcc ggcaggccct cccccgcgct
      601 ccaggagctg gcggcggccc ggcccaacgt gtccgtgatc accgtcgagc cgggcaccgt
      661 cccggcgccc gagccggtcg acctggtctg gacgacgctc aactatcacg atttcaagaa
      721 cgcgaaggtc ggcgagggcg acggcgccgc ggcgatcaac gccgccgcct tccgtgcgct
      781 gaagcccggc ggcgtctatc tcgtcgtcga ccacgacacg gcgccgggcg cgggggcctc
      841 ccagacctcc accctccacc gcatcgaaag cgcgctggtc cgccgcgagg tcgagagcgc
      901 gggtttccgg ctggaagccg aaagcagcct gcttcgccat cccgaggacg atcacagcgc
      961 cagggtccag gagacgggaa tccgcggcaa gaccgaccag ttcgtcctcc gcttccgaaa
     1021 gccccgctga tcgccgtgac atccggcgtc cggcgccgaa ttccctccgg ctcgccttcg
     1081 cgaccctcgt cgccctcgac atccccatca cctcgatcct gatgtcgggc cgaaccgtcg
     1141 gcccgccgtc gagcgatggt taaccggcct tttaccatcc ctctttacca cggccggcgg
     1201 gaagaggggg atctgaccaa tgatcgggat agctcttatg gccgccgccg cgcatgcgcc
     1261 ggtgctgctg gccgccaacg acgaagccca gtggcgccgg ttcgtgcgcg ccagcctgca
     1321 gcgtccggcc gcgccggccg ctcgggcgcc gtcccggctg aaccagctgc agggcgtcgt
     1381 gcgccgactg gacgacgacc gctccggccc ggcctcggcg tccgcgggcc cctacggcct
     1441 gtagaaaccc gtcgtttgaa cgcggctgac ccgactgcgg tcaggcgcgg ttgcggacgt
     1501 agcttccggg agcgggctcg agcgccttga gcttcccgtc gccgggcgcg cgcgccttca
     1561 ccttttcggc cttgccgtcc tcgctgagcc gcgcctgcca ccagggccac cagctgcctt
     1621 cgcggcgctc ggcgccctcc agccattgct ccgccgtctc gggcatcgcc gcgttcaccc
     1681 aatagccgtg cttgttggcg ctgggcgggt tgatgacccc ggcattgtgg ccgcttccgc
     1741 cgagcaggaa ggcgacgtcg ccgccgaaca ggcgggcgct gtcgtaggtc gcctgccacg
     1801 ccgagacatg atcgtccttg aggctgatcg cgagcagggg cgtcttcacc gcgccgagct
     1861 cgatcctcac cccgtccagc tccaggccgc ccttggccag cttgttgtcc ttgaggatat
     1921 ccttggccca ttcgagcagg aacgcgcgcg ggatatgggc gccgtcggcg aaccagtgga
     1981 gaatgtcgct gggcggcgcc tcgcggtcga gcagatagtg gttgaccacc gacgaccaga
     2041 tcaggtcgtt ggcccgcacg gcggagaaca aggcctggag atcctgcgcg gccatgaagc
     2101 ccttgtggag gaggtggcgc tccatcgccc gaagctgctc gggctccgag aaggtcgccc
     2161 attggccgag gcgcgagaaa tcgaacatcg ttccgatcgt ggtggcggag gcgacccggc
     2221 cgccctcccc tttcgcggcc agccaggcga gcgcgatcgc ggcgagggtg ccgcccatgc
     2281 agaaaccgaa caggtcgacc tcgcgctcgc cggtggcggc ctcgatcgcg tccagggcct
     2341 cgagcgggcc ctggcggatg tagtccgcga tcgccttgtc ggcgagctcc gggccggggt
     2401 tgacccagga gatgacgaag accgtatggc cttgctccac cagccaccgc atcaaggagg
     2461 acttgggctg caggtcgagc aaataatatt tgttcaccag cggcgggacg tagaggatcg
     2521 ggcggcggta gacctggtcg gtcgccggcg catattggat cagctccatc agcgcgttgc
     2581 ggtagacgac gctgccggga gtcgcggcga tgtcgacgcc gagctcgaac tcccgctcgg
     2641 cgcggcgctg gacgaggccg ccctgggccg cgtcggcgag caggttcgcc aggcccgaga
     2701 gcagcgagat tgaaccggtg tcgatcgcct tgcggatcgc ctcggggttg gtgaaggcga
     2761 aattggccgg cgagatcgca ttcagatatt gctgggtgaa gaactcgact cgggttcgag
     2821 cggattcgtc cagcccttcg gcctcctcga ccatctcgag cgcccgtttg gcggtcagca
     2881 gataggcctg cttcagatag tcgaagaccg gctcgtcgct ccaggcggga tcgttgaagc
     2941 ggcggtcgcc ccgctcgggc acggcaacgg gatcggcggc acggccggcg gcgcgcgcgg
     3001 cggccgattg ccacagcttc atccagtcgg acgccgcctt ctgctgcgcc ttgacgagct
     3061 cttgcggatg gctccacagg tgcgccgcga actcgccgaa cgcccgcgcc ggcgccgcgg
     3121 cgtcgaacgg ctgggggacg tgcgcgtcga gcgggcgcgc ggccgcgctg gtggcgagat
     3181 ggaccgcgtt ggcgacgacc ttggaccagg cctcggcgag gtcggcgggg ctcggcaggg
     3241 attcgggaag cttgaagacg gtcgaggggg ggtctgacat ctgcatcctc ttgcgctcat
     3301 cctgagtagg gactgagcct ggcgaaggcc cgtatcgaag gacggtcctt cgatgcgccg
     3361 cttcgacctc gctcagcggc tactcaggat gagcgactgg ggcaatcccg ctctcagacg
     3421 cttacagcgc catcagctcc tcgagcgcgc cgcagtcgcg agggctgacg aagagaaaga
     3481 gcatggcccg gtccgggttc cgcgcggccc agccggcctc ggggtcggcc ggcgtgcgga
     3541 aatccgcagc cgcgacggac gcgggggcct gatgattggc aagcacagcg gcggtccgag
     3601 cctcgaacat ggattgtctg cctctcgatg ggatggtctc ctctctcaca cgaagcgctc
     3661 gacttccatc gggattgtgc cgaaaccgtt gtgcatatat ccccataccc atggccgaat
     3721 tcgactggaa cgacgtccgc gccttcctcg ccgtcgcccg gagcggccgg ctgacggcgg
     3781 cggcgggtcg gctgggagtc gaccattcga cgctgtcgcg ccggatcgcc gcgctcgagc
     3841 atgccctgag atcgaagctg ttcgaccgga gcccgtcggg ctacaccctg accgaggaag
     3901 gccggcggct gctcccgatc ggcgaggaga tggagcgcct ggcgatcggc gccggcgagg
     3961 cgatcggcgg cacctccacc tcggtggccg ggacggtgcg gatcggctcc ccggaggggt
     4021 tcggcagcta tttcctcgcg ccgcggatcg ccgggctgaa ggcgcgccat ccgcggctca
     4081 ccatccagct ggtcgcggcc ccggccgtgt tcagcctcgc ccggcgcgac gcggacgtcg
     4141 tcatctcggt cagccggccg ccggcgggac ggctccatgt gtcgaagctg atcgactacg
     4201 atctcggcct ctacgccgcc cccggctacc tgcggcaggc ccccgacatt gcctcggccg
     4261 gggatctctc ccgccaccgc ttcgtcagct atatcggcga gctcctccat ttcccggagc
     4321 tcgacttcct ccagcatgtc gcgccgggag gcaccacctc ggtcgagagc agcaatctcg
     4381 tcgcgcagct cagggcgacg ctggccggag ccgggctgtg cgtgctcccc gccttcctcg
     4441 ccggcgagga ggacgggctg gtgcgcgtgc ttcccggcga gatcaacctg acgcgctcgc
     4501 tctggctgac cgtccaccag gacctggcgg agctcgcgcg ggtgcgcgcc gcggtcgcct
     4561 tcatccggga ggaggtcgaa agcgctcggg gcctgttcaa gctctaggcg agggcccggt
     4621 aaaggccgaa gctgctgctc gcggtcagga cgatcccgac gaaggtgagc aagcggtccg
     4681 cgtcgacctt cttggcgatc caggcgccga agggcgccgc catcacgccg ccgatcagga
     4741 gcccgagggt ggcgacggtg aacgcttccc agcccagcgt cgcgatgaag gtcgccgaga
     4801 tggtcacggt caggaagaat tcggcggtgt tgaccgtgcc gatcgtcttc ctcgggctcg
     4861 atccctggac cagcaggttc gaggtcacca ccgcgcccca gccgccgccg ccggccgcat
     4921 cgaggaagcc gccggcgaga ccgagcggcg cgacgatcct cggctcgcgc tcgttgtggc
     4981 ggtgcatcac cccgcggtag agcagataga ggccgagcgc ggcgagatag gcgaggacga
     5041 tcggtttggc ggtcgaggcg tcgatattgc tgagcacata ggcgccgatc acgccgccaa
     5101 tcaccccggg gacggcgagt cggaagaaga gccgccagtc gacgttgcgg tggaggacgt
     5161 ggctgacgcc cgagaccgcg gtggtgaagg tctccgccgt atgcacgccc gccgaggccg
     5221 cggcgggcgg cacgcccatg ctgatcagca gggtggacga gatctggccg aaggccatgc
     5281 cgagcgcgcc gtcgatgagc tgggcggcga agccgatcgc gatgaagggc aggatgtcga
     5341 gcagccccac gcccccaagg tcgaacatgc ccgctccctg ctgccgatcg catcgggatg
     5401 tgctgggcgg ccgcttccgc gcaagcgaaa aagcgccggt aacggaattt tggcgctcgg
     5461 ctggcatttt cctgcggtcg gccctatttt gccgggcacg gagcggagtg ggagtggcta
     5521 tgctgccggg ccttgtcgcc tatctcgatt cagtgaaggc ccgcgatccc gctccacgca
     5581 gccgctggga agtgctgctc tatccgggcg tctgggcgct cggcttccat cgcctggccc
     5641 actggctcta cagcggcgag ctctatttcc tcgcccggct cgtcaaccat ctgggccgct
     5701 tcctgaccgc gatcgacatc catcccggag cctcggtcgg tcgcaacttc ttcatcgacc
     5761 acggcttcgt cgtcatcggc gagacggcga cgatcggcga cgacgtcacc atctaccagc
     5821 aggtcaccct gggcgggacc aaccccaccg acggcatccc cggcaagcgg cacccgtcga
     5881 tcggcaacca cgtcatcatc ggctcgggcg cgcaggtgct ggggccgatc atcgtcggcg
     5941 agcgggcccg catcggcgcg aacgcggtgg tcaccaagga tgtgcccgaa ggcgcgacca
     6001 tgaccggcat cccggcccgc tccactctgc tcagcgccga gcagagcagc tccggcaatt
     6061 tcctccccta cggcacgccc tgctccgagc agtgcgatcc ggccgctcag aagctggaag
     6121 cgctccagca cgaggtggag cagctccgcc gccgggtcgc cgacctgatc gaggagcgcg
     6181 acggcaagac gagaaaggag cgcggccgcg cctgatgggt accgtccacc cttttccggg
     6241 agcctcgtcg ccgcagatcg gcttcacccg gtccgagctg aagcggatca tcgacctcta
     6301 cggacggatg gtcgcggccg gcctgtggcg cgactatgcg gtcgagttcg gccgcgacgg
     6361 cgccgccttc tgggccttcc gccgaaccgc ggagcggccc gaatacaaga tcgagaagcg
     6421 tccggcgctg cgtaaccgcc agggcctttg ggcgctggtc aacgaggccg gcatggtcat
     6481 ccgccgaggc gacgagctcg gcccggtgct ggctccgctc gagcggcggc tcctcaaggt
     6541 ggtcaacgga taaaagaaat cctccccatc gtcagatggg gagggggacc aggcgaagcc
     6601 tggtggaggg gtagctgtct caggcttgtg ccagttaccc ctccaccacc cttcgggtgg
     6661 tccccctccc cgcgctccgc gcggggagga tgatcaggcc gccgccgcag ccggggccgc
     6721 gcgatcgaga accggcaggc gctcctgcag gcccggcggc aggggccgga cgatcgagcg
     6781 gcggacgctg tgccagaagg ccgagagcag cagcaggccg gagccgatca cgaacgccgc
     6841 cagggcgatg ttgagctcga ccgcgccgaa ctcgcggaac aggctgatca gggcgaacag
     6901 cacgtaggcg agggccgaga cgagcagcgc ccgacggtcg atcgccagcg cggtgatgcc
     6961 gagcagcaca tagactccga ggacgaccag ggcctccccg atgctggcgt cgccatcggt
     7021 gagtccgagc agcgagaaga tcgggtggac gatcatcggc gccgccagca ggtgaagcca
     7081 gaaggcgacg tcggagcgac gggtctggcg cgctcggtcg gacgaatccc accacatcgc
     7141 gaacaggaac acgccgaggc cgaggaccag gccgctggcg agcatgatgt cctctagggc
     7201 ggggacgtcg ccgaccgccg aggcgatcag cgccatgacg accccgacca cggtgagcgc
     7261 tccggcggcg acggtgatcg gcactcggaa ccggcgccag tgcatccagg tggcggcggc
     7321 ggcgaccgcg gccgaaccgg cgatgacgag ggccaccgcg cgctcgttat tctcgaaggt
     7381 cgcctcgccg agggcgagga cgagcgcgga gaaggtggtc agaagcacgc ctccggtgaa
     7441 ggcgagaaga agcaggatgc tggggagcgc catgcgcctc ttcttggtga agaattcggc
     7501 cagcccccag gcggtgccgg cgaccagcag gccggcgaag ggcgacgggc catcgccgtc
     7561 gatattggcg cccatcgaat tgccgatcca gccgaccgcg aacagcagga tcgcggcggc
     7621 gatgctgacg aagatgtcgt tgaagccggt gatgaggcgg aaatgctcct cgtcgacggg
     7681 cgagctcgtc gcccggctgt ccatataggc gcggagcgcg gaggcggatt cggggcttag
     7741 ggccccggcc gcgaccgccg cgtccagatc gctgtcgctg tacatatggt tttctcctcc
     7801 caggatcgga ggctcttagc acaggtgtat tagtgttaca atacggtaac gtccctcccc
     7861 gagatttccg gggcggtgga ggggttcttc tgagtcggca aggtcccctc caccatgctt
     7921 cgcatggtgc ccccccgtgc aaatacaggg gaggaattaa ttcccctgca acgtcttgag
     7981 gatcgtcatc gcctgctccg caccggatcc ggggacgacc tcgagcgtcc gatcggtgac
     8041 cttgtcccag ttcgccgcca gctcctcgac cgtccggcgg ccctcgggca gagcgtagcc
     8101 gggcgtcagc gtgacatagg ccgcctgcac gacgccgccg cccgcgccga cgatcgcgcc
     8161 cgacggcgct tcctccgaaa ccagatagag cgcggccggg acgacgttct gcgggttgaa
     8221 caggctcagc atctccggcg gaaacaggtc ctcggtcatc ctcgtcgccg ccgtcggagc
     8281 gatggtgttc acccggatgt cgtatttggc gccttcgaga tggagcgtcc gggcgaggcc
     8341 ggccaggccc atcttggccg ccccgtaatt ggcctggccg aaattgccgt agaggccggt
     8401 cgagctcgac gtcatcagga tccggccata ggcctgctcg cgcatcgtct cccacacggc
     8461 cttggtgcag accgccgagc cgatcaggtg gacgtcgacc accgcccgga aatcctcgac
     8521 gctcatcttg gcgaagctct tgtcgcgcag gatgccggca ttgttgatca ggatgtggac
     8581 cccgccccag cgctccttcg cggcggcgac catcgcctcg acctgggccg agtcggtgac
     8641 gctggccccg ttggcgatcg cctccccgcc ggcggcctcg atctcctcga cgacccgctg
     8701 cgccgcgtcc gagcgcccgg aaccgtcgcg ggcgccgccg aggtcgttga ccacgaccct
     8761 ggcgccgcgg cgggcgagct cgagcgcata ggcgcggccg aggccgccgc cggcgccggt
     8821 gacgatggcc actcggcctt caaagctgat ggtcatggat cctccgcgat gcccttcttc
     8881 c
//
LOCUS       DASVQZ010000353         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_844051, whole genome shotgun sequence.
ACCESSION   DASVQZ010000353 DASVQZ010000000
VERSION     DASVQZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844051"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VF547_10100"
     CDS             complement(<1..464)
                     /locus_tag="VF547_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8263213.1"
                     /translation="MATPAPGAAPGGGDNPYGLMAALQQGGAIAWATFIILVIMSAGS
                     WYVLFVKLFEQQKIINQGRRARTTFWQSANLREGAAKLEKNSAYRQIVDDGILALEQH
                     DKLTDPIDQHDWMLNSLARSQGAITSRLSSGLAFLATVGSTAPFVGLFGTVVG"
     gene            complement(523..1200)
                     /locus_tag="VF547_10105"
     CDS             complement(523..1200)
                     /locus_tag="VF547_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8263214.1"
                     /translation="MAYADNRMSRSRMTAIFIVALLHALLGYALITGLAYNVVKKVAA
                     DLKTFNVEEEPPPPEEKPPPPPPDQPQTPPPVVTPPPIVRTNTMPPPIVTTNVAPPPI
                     ITPTAPPAPPAPPAPPPPPPPPPKKVQPARAKANLVSLFSDEDYPASAVRAGESGATG
                     FRLDVGPNGRVANCTVTSSSGSSALDSTTCRLLKSRARFSPATDSTGAATSDTVTGRI
                     VWRLPAD"
     gene            1458..2300
                     /locus_tag="VF547_10110"
     CDS             1458..2300
                     /locus_tag="VF547_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263215.1"
                     /translation="MKRLLTFCRWTPLAALLAAGAPSAESQVPTAPSPTYADLADLAL
                     AAPVAAQVRVADSVAVKAERAPGLRPGFARFYVEAVLVSLIRAPQTLPARLSYLVDLP
                     LDAKGKAARPRKGSEFLLLAAPVAGRPAELRLVAPDAQIPATPERAATLRAIVREASS
                     ASPPPRIAGIGRAFHVPGAVRGESETQIFLQTAGGTPVSLTVLRRPGQTPQWAVALSE
                     IVDEAAEPPAPGTLLWYRLACGLPRSLPRQSLAEAGEHVAAIEADYRLVLERLGPCVR
                     TRRS"
BASE COUNT          363 a          853 c          896 g          407 t
ORIGIN      
        1 ccgacgacgg ttccgaacag gccgacgaac ggcgcggtcg agccgacggt ggcgaggaag
       61 gcgaggccgc tcgacagacg ggacgtgatc gcgccctggc tgcgcgccag cgagttgagc
      121 atccagtcgt gctggtcgat cgggtcggtc agcttgtcat gctgctcgag cgcgaggatg
      181 ccgtcgtcga cgatctgccg gtaggcgctg ttcttctcga gcttcgcggc gccttcgcga
      241 aggttcgccg attgccagaa ggtggtccgc gcgcggcggc cctggttgat gatcttctgc
      301 tgctcgaaca gcttgacgaa gagcacgtac cacgagcccg ccgacatgat cacgaggatg
      361 atgaaggtgg cccaggcgat cgcgccgcct tgctggaggg cggccatcag gccgtagggg
      421 ttgtcgccgc cgccgggggc tgcgcctggt gctggggtgg ccatggtctc tactttcctc
      481 tcagataaac gttttggatc gtgattgccc gccgactcgt catcagtcgg ccgggagacg
      541 ccagacgata cggccggtca ccgtgtcgct cgtcgccgct ccggtgctgt cggtcgccgg
      601 cgagaaacgc gcgcgggact tgagcagccg gcaggtcgtc gagtcgagcg ccgacgagcc
      661 gctggacgag gtcaccgtgc aattggcgac ccggccgttg gggccgacgt ccagcctgaa
      721 gcccgtggct cccgattccc cggcgcggac cgccgaagcc ggatagtcct cgtccgagaa
      781 gagcgacacc aggttcgcct tggcgcgggc gggctgcacc ttcttgggcg ggggcggagg
      841 cggcggcggc ggggccggcg gcgccggggg cgccggcgga gcggtcggag taatgatcgg
      901 cggcggcgcg acgttcgtcg tcacgatcgg cggcggcatc gtgttggtcc gaacgatcgg
      961 cggcggagtc accaccggcg gcggcgtctg cggctggtcg ggcggcgggg gcggcggctt
     1021 ctcctcgggg ggcggcggct cctcctcgac gttgaacgtc ttgaggtccg cggccacctt
     1081 cttgacgaca ttataggcga ggccggtgat cagtgcgtaa cctagcagag catggaggag
     1141 agcgacgatg aagatcgccg tcatgcggct ccggctcatc ctgttgtcag catatgccat
     1201 taatcagcca cactccctct ctctcgttct cgccggggat tttcggcgcc accatcaacg
     1261 cggctgatcc ccaacggctc catgacgacg gcaatgtatc aacatatcat cgactggagc
     1321 gggtccatct atcgtgagac aggggacgac gcaatcctcc tgtggataaa gggcgacggc
     1381 cgcggaaacc gctgaaatcc gcggttcaga cggctaagcc tatgggatac cgggcataaa
     1441 ctttcggagg cggaacggtg aagcgcttgc taactttttg tcgatggacg ccgctggcgg
     1501 ccttgctggc ggccggcgcg ccttccgccg aatcacaggt gccgaccgct ccgtcgccca
     1561 cctatgccga cctcgcggac ctcgcgctgg ccgcgccggt cgcagcccag gtccgagtcg
     1621 ccgactccgt cgccgtcaag gcggagcgcg cgccggggct ccggcctggc ttcgcgcgat
     1681 tctacgtcga ggcggttctg gtctcgctga ttcgggcgcc gcagaccctc ccggcccggc
     1741 tcagctacct cgtcgacctg ccgctggacg ccaagggcaa ggccgccagg ccgcgcaagg
     1801 gcagcgagtt cctgctgctc gccgcgccgg tggccggccg gcccgccgag ctccggctgg
     1861 tcgctccgga cgctcagata ccggccacgc ccgaacgggc cgcgaccctg cgcgcgatcg
     1921 tccgcgaggc gagctccgcc tcgccgccgc cgcgaatcgc cggcatcggc cgcgccttcc
     1981 acgtcccggg cgcggtgcgc ggcgaaagcg agacccagat cttcctgcag acggccggcg
     2041 ggacgcccgt gtcgctgacc gtcctgcgcc ggccgggcca gactccccaa tgggcggtgg
     2101 cgctgagcga gatcgtcgac gaggcggccg agccgcccgc gcccggcacc ttgctctggt
     2161 accgactcgc ctgcgggctg ccgcggtcgc tgccgaggca gagcctggcc gaggctggcg
     2221 agcatgtcgc cgccatcgag gccgactacc ggctggtgct cgagcggctc gggccgtgcg
     2281 ttcggactcg ccgctcttaa ttcctccccg cgatttcgcg gggaggggga ccgcgcgaag
     2341 cgcggtggag gggttctttg agctcagcaa ggcccctcca ccaggcttcg cctggttccc
     2401 ctcccctgcc aatgcagggg aggaattcaa taggacgtaa gctcgacgcc gactccggcg
     2461 caatcgggat agacgtcggg cttgcggatc gcgacccgaa gcgccgtcac cccctgccg
//
LOCUS       DASVQZ010000354         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_653130, whole genome shotgun sequence.
ACCESSION   DASVQZ010000354 DASVQZ010000000
VERSION     DASVQZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653130"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1189)
                     /gene="tilS"
                     /locus_tag="VF547_10115"
     CDS             complement(221..1189)
                     /gene="tilS"
                     /locus_tag="VF547_10115"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX8263216.1"
                     /translation="MAAAPSPEQVDRFRRDLERLAGSAPAVAVAVSGGPDSLALLLLA
                     HAAWPGRVKAATVDHGLRSGSADEALEVARLCGRLDVPHRILAARVDRSGEGLQAAAR
                     KARYAALARWMDEDGLDLLLTAHHADDQAETLLMRLNRGSGVAGLAGVRAYGPVPGTR
                     GRLRLCRPLLGWRRSALEAIVAAAGLEPARDPSNEDEAFDRARLRRQLGEAGWLDPDA
                     LARSAALIAEAEAALEWTAARLFAARAGEAEGLLTLRAQGFPPELQRRLVLRCLRRLA
                     PAARPRGEPLAALVGRLRDGGTATLCGVKATGGETWRFERAPPRRR"
     gene            1304..>2425
                     /locus_tag="VF547_10120"
     CDS             1304..>2425
                     /locus_tag="VF547_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8263217.1"
                     /translation="MAFPRAFVLALLAGTAASPLLAQQPAAPAPAAAPPAPDAPAEAA
                     AAAQVSAEPADPDAELDDEEDGETIVVTGQRQRGAVIGDIPPEVQFDRREIRALGAGS
                     LSELLDAIAPQTRSGRGRDGGRPVILLNGRRISGFAEIRDIPPEAIVRIDVMPEEVAL
                     KYGYRADQRVVNFVLRRRFDAYTTEVQGGLATAGGRESYGAELNYLQINRAGRINLGA
                     EYRRAEPLFESERDIVQAEPMAGIELGRFRTLLPQTDQLELSGTLNRAVSETVSATLN
                     GTFDVNSSTAFLGLPRPSLEPPGLRRETDTRAGHLGLSVNGAMAPWQWTLTANFDRSA
                     SETVTQTSDGTDQSRSVSQIGIAELLMNGPLFELPGGSVT"
BASE COUNT          348 a          915 c          861 g          301 t
ORIGIN      
        1 ccgccaggaa ggagagacgg tcggccggct cgatgtcctc gctggtgcgg tcggcgagct
       61 tgtcgagccg gttgctcagc tcctcgacgc tgttcgcgcg cggctcgacc tcgtcgccgg
      121 agccgccgca ggccccgagc aggagcagga tcgccaggga aggccgaatc gccatggcgc
      181 ttcaacccgg aatgggggcg actcgatccc gagcggggtt ctagcggcgg cgcggcggcg
      241 ccctttcgaa gcgccaggtc tcgccgccgg tcgccttgac tccgcaaagg gtcgccgttc
      301 cgccgtcgcg caggcggccg accagagcgg cgaggggctc cccgcgcggc ctcgccgccg
      361 gcgccaggcg ccgcaggcag cgcagcacca gccggcgctg gagctcggga ggaaagccct
      421 gcgcgcgcag ggtgaggagt ccctccgcct ccccggcgcg ggcggcgaac agccgggcgg
      481 cggtccattc gagcgcggcc tccgcctcgg cgatcagcgc ggcgctgcgg gccagggcgt
      541 cgggatcgag ccagccggcc tcgcccagct gccggcgcag ccgtgcgcga tcgaaggctt
      601 cgtcctcgtt gctcgggtcg cgcgccggct cgaggcccgc ggcggcgacg atcgcctcga
      661 gcgcgctccg ccgccagccg agcagcggcc ggcacaggcg cagccgcccg cgcgtgcccg
      721 gaaccggacc ataggcccgg acgcccgcga gcccggccac tccggacccg cggttgagcc
      781 gcatcagcag ggtctcggcc tggtcgtccg catggtgcgc ggtgagcagg agatcgaggc
      841 cgtcctcgtc catccatcgc gccagagcgg cgtagcgggc cttgcgcgcg gcggcctgaa
      901 ggccttcgcc cgagcggtcg acgcgcgcgg cgaggattcg gtgggggacg tcgagccggc
      961 cgcacagacg ggcgacctcc agcgcctcgt ccgcactccc gctacgcagg ccatgatcca
     1021 cggtcgcggc cttgacccgt cccggccagg cggcatgggc caggagcagc agggcgaggc
     1081 tgtcgggccc gcccgagacg gcgaccgcga ccgccggggc ggagcccgcc agccgctcga
     1141 ggtcgcggcg gaagcgatcg acttgctccg gggaaggtgc ggcggccatc ggctctcctt
     1201 aacgctggga agctgccgga tacaggatgc gttcagcccg tctggcccag cccgtcgcct
     1261 tgccctgtcg ggcctgaaaa acaaaatcaa aggtaagcat cgcgtggctt ttcccagagc
     1321 gttcgtcctc gcccttctcg ccggaaccgc cgcctcgccc ctgctggcgc agcagccggc
     1381 ggctccggcg ccggcggccg ctccaccggc ccccgacgcc cccgccgagg cggccgccgc
     1441 ggcccaggtc tcggccgagc ccgcggatcc ggacgccgag ctcgacgacg aggaggacgg
     1501 cgagacgatc gtcgtcaccg gccagcgcca gcgcggcgcg gtgatcggcg acatcccccc
     1561 cgaggtccag ttcgaccgcc gcgagatacg cgcgctgggc gcgggaagcc tctccgagct
     1621 gctcgacgcc atcgctccgc agacccgaag cggccgcggc cgcgacggcg gccggccggt
     1681 catcctgctc aacggccgcc gcatctccgg cttcgccgag atacgcgaca tcccgcccga
     1741 ggcgatcgtc cggatcgacg tcatgcccga ggaggtcgcg ctcaaatatg gctatcgcgc
     1801 cgaccaaagg gtggtgaact tcgtcctgag gcggcgcttc gacgcctaca cgacggaggt
     1861 gcagggcggc ctcgccaccg ccggcggccg cgaatcctac ggcgccgagc tcaactacct
     1921 gcagatcaac cgggccggac ggatcaacct gggcgccgaa tatcgccgcg ccgagccgct
     1981 cttcgagagc gagcgcgaca tcgtccaggc cgagccgatg gccgggatcg agctcggccg
     2041 tttccgcacc ctgcttccgc agaccgacca gctggagctg agcggcacgc tgaaccgggc
     2101 cgtctccgag acggtttcgg cgactctgaa cggcaccttc gacgtcaatt ccagcaccgc
     2161 cttcctcggc cttcccaggc cgagcctcga gccgcccggc ctgaggcgcg agaccgacac
     2221 gcgcgccggc catctcggcc tttcggtcaa cggggcgatg gcgccgtggc aatggacctt
     2281 gaccgccaat ttcgaccgaa gcgcgagtga gacggtcacc cagacgagcg acggcaccga
     2341 ccagtcacgc tcggtcagcc agatcggcat tgccgagctg ctgatgaacg ggccgctgtt
     2401 cgagcttccc ggcggcagcg tcacc
//
LOCUS       DASVQZ010000355         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_174712, whole genome shotgun sequence.
ACCESSION   DASVQZ010000355 DASVQZ010000000
VERSION     DASVQZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174712"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..339
                     /locus_tag="VF547_10125"
                     /pseudo
     CDS             1..339
                     /locus_tag="VF547_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            544..1026
                     /gene="rimM"
                     /locus_tag="VF547_10130"
     CDS             544..1026
                     /gene="rimM"
                     /locus_tag="VF547_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404075.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX8263218.1"
                     /translation="MAEDQVALAAIAGAHGIGGEVRLKLFAESADSLKRHQRVRVGER
                     HLTLASLKGDRALIARFAEIADRTAAEALRGQLVTVPRSALPPLEEGEYYHADLIGLA
                     CETAAGEPLGRVVAVEDFGAGDILEVEKPDGRRTMVPFREGIADLVGGRIVADPEFLA
                     "
     gene            complement(1023..1973)
                     /locus_tag="VF547_10135"
     CDS             complement(1023..1973)
                     /locus_tag="VF547_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8263219.1"
                     /translation="MSEPDFDCLIVGGGPAGLTAAIYLTRFHLTAKLVDAGKSRASLI
                     PCTHNHAGYPDGISGQELIARMKAQAQKYGTSIVTSRVTRLDRTEAGFRAEWGAGSGV
                     ARKVLLATGVTNRRPPMDEDLHDEALARGLIRYCPICDGYEVTDKAVGVIGDGSHGVA
                     EAVFLRGFSEDITLIAPDTAHKLSAEDQARLREYGIRTVDGPCSAVAAHDDCIVVDTP
                     DGHYTFDSVYPALGSDMHNELAGQVGAELKDGNIVVDSHQRTSVPGLYAAGDIVLGLD
                     QISHAMGEGGVAAVTIRNDLAKERHIRREALSGSIRAKGG"
     gene            2047..2793
                     /gene="trmD"
                     /locus_tag="VF547_10140"
     CDS             2047..2793
                     /gene="trmD"
                     /locus_tag="VF547_10140"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8263220.1"
                     /translation="MTWSATALTLYPEMFPGPLGVSLAGRALEQGIWRLETVQIRDYA
                     TDRHRSVDDTPAGGGAGMVMRADVLGAAVDHVLAQRPDAPVVAMTPRGKPLTQDFVRE
                     LAAGPGVSILCGRFEGIDERLFEARPIVPVSVGDYVLSGGEIGALVLLDACVRLLPGV
                     MGAPSSGVEESFESGLLEYPHYTRPVEWEGRTIPEVLRSGDHARIAAWRESRAAADTR
                     SRRPDLIERHRDARRPPPSGARREEESEET"
     gene            2790..>2990
                     /locus_tag="VF547_10145"
     CDS             2790..>2990
                     /locus_tag="VF547_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX8263221.1"
                     /translation="MNLIQQLEAEAIEQATQGRPIPEFRPGDTVRVGVKVVEGERTRI
                     QNFEGVCIARANRGLGSSFTVRK"
BASE COUNT          447 a         1032 c         1092 g          419 t
ORIGIN      
        1 ccgctgcttc ccaaggacag cccggaccgg gtgaagctcg acgcggaccg ggcccgccac
       61 tggctgtcgg tcggcgccca gccgagcgac cgggtcgccc gcttcctcga cgccgccgga
      121 gtccgcgagc gcgcggcgcg caacaacccg aacaagggcg tcccgggcga caaggccaag
      181 gagcgcgccg aggagcgcgc cgcccgcgag gccgaagccg ccgaagccgc cgccgccgag
      241 gaagcggccc ccgccgccga ggaagctccg gccgcggacg aggtcgtcgc ggaggaagcc
      301 gccgcggaga cgcccgccgc cgaggaggct cccgccgccc aggaggctcc cgcggccgag
      361 gaagcgcctg ccgccgagga ggctcccgcc gaggaagcgc ctgccgccga ggaggcttcg
      421 gcggacgaag gcgaggccac cccggccgac gaggcttcga acgagggcgt ctcgaccagc
      481 gagccggccg aagggtccga cgaggccgcc ccggccgagg gcgccgaaaa ggcggaatag
      541 cctttggccg aggaccaggt cgccctcgcc gccattgccg gagcgcacgg catcggcggc
      601 gaggtgcgct tgaagctctt tgccgaaagc gccgacagcc tgaagcgcca tcagcgggtg
      661 agggtgggag agcgccacct caccctcgcc tcgctcaagg gcgatcgcgc cctgatcgcc
      721 cgcttcgccg aaatcgccga ccggaccgcc gccgaggcgt tgcgcggcca attggtgacc
      781 gtgccgcgct cggcgctgcc gccgctcgag gaaggcgaat attatcacgc cgacctgatc
      841 ggccttgctt gcgagacggc ggccggcgag ccgctgggcc gggtcgtcgc ggtcgaggat
      901 ttcggcgccg gcgacattct cgaggtcgag aagccggacg gccggcgcac gatggtgccg
      961 ttccgtgagg ggatcgccga tctcgtcggg ggccggatcg tcgcggatcc ggagttcctg
     1021 gcctagccgc ctttggcccg gatcgagccg ctcagggcct cgcgccggat atggcgctcc
     1081 ttggccaggt cgttgcggat ggtgacggcc gccaccccgc cctcgcccat ggcgtggctg
     1141 atctggtcga gtccgaggac gatgtcgccg gcggcgtaga ggcccgggac gctggtgcgc
     1201 tgatggctgt cgaccacgat gttgccgtcc ttcagctccg ccccgacctg gccggcgagc
     1261 tcgttgtgca tgtcggagcc gagcgccggg tagacgctgt cgaacgtata atggccgtcg
     1321 ggggtgtcga cgacgatgca gtcgtcgtgg gccgccacgg cggagcaggg gccgtcgacg
     1381 gtgcggatgc catattcccg gagccgcgcc tggtcctccg cgctgagctt gtgggcggtg
     1441 tcaggcgcga tcagggtgat atcctcggag aagcccctca ggaaaaccgc ctccgccacc
     1501 ccgtgcgagc cgtcgccgat gacgcccacc gccttgtcgg tgacttcgta gccgtcgcag
     1561 atcggacagt agcgtatcag gccgcgggcg agcgcctcgt cgtgcagatc ttcgtccatg
     1621 ggcggacggc ggttggtgac tccggtcgcg agcaggacct tgcgcgcaac tcccgatccc
     1681 gccccccatt cggcacggaa gccggcttcg gtgcggtcca ggcgggtcac tcggctggtg
     1741 acgatcgagg tgccgtattt ctgcgcctgc gccttcatcc gggcgatgag ctcctgcccg
     1801 gaaatgccgt cgggatagcc ggcatgattg tgggtgcagg ggatcagaga cgcgcggctc
     1861 ttgccggcat cgaccagctt tgcggtgagg tggaagcggg tgagatagat ggcggcggtc
     1921 aggccggcgg ggccgccgcc gacgatcagg cagtcgaaat cgggttcgct catggggcct
     1981 cagcgttctg gcgcagccac ggttccgcgg cttgcggcgg aggcggggag gggcgaaagg
     2041 aggtccatga cctggagcgc gaccgccctt accctctatc ccgagatgtt tcccggtccg
     2101 ctcggcgtca gccttgccgg acgggcgctg gaacagggga tttggcggct ggagacggtc
     2161 cagatccgcg actatgcgac cgaccggcac cgctccgtcg acgacacgcc ggcggggggc
     2221 ggcgccggga tggtgatgcg cgcggacgtt ctcggcgccg ccgtcgacca cgtcctggcc
     2281 cagcgccccg acgccccggt cgtcgccatg accccccgcg gcaagccgct cacccaggac
     2341 ttcgtccgcg agctcgcggc cggccccgga gtctcgatcc tgtgcggccg cttcgagggg
     2401 atcgacgaac gattgttcga ggcccgtccc atcgtcccgg tgtccgtcgg cgactatgtc
     2461 ctgtccggcg gcgagatcgg cgccctggtc ctgctcgacg cttgcgttcg gctgctcccc
     2521 ggggtaatgg gcgcgccttc aagcggagtg gaggaaagct tcgagagcgg gcttctcgaa
     2581 tatccgcatt atacccgacc tgtcgaatgg gaagggcgca cgatccctga agtgctgcga
     2641 tcgggggatc atgcgaggat cgccgcctgg cgcgagagcc gggcagcggc cgacacacgg
     2701 tcaaggcggc cggacctgat cgagcgtcac agggacgcgc gaaggccacc gccctctggc
     2761 gcgcggcgcg aagaagagag cgaagagaca tgaacctcat ccagcagctc gaggccgaag
     2821 ccatcgagca ggccacccag ggccgcccga tccccgagtt ccgccccggc gacaccgtcc
     2881 gcgtcggagt caaggtcgtc gaaggcgagc gcacccggat ccagaatttc gagggcgtct
     2941 gcatcgcgcg cgccaaccgg ggcctcggct cctccttcac cgtccgcaag
//
LOCUS       DASVQZ010000356         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_555891, whole genome shotgun sequence.
ACCESSION   DASVQZ010000356 DASVQZ010000000
VERSION     DASVQZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555891"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF547_10150"
     CDS             <1..213
                     /locus_tag="VF547_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX8263222.1"
                     /translation="EVAGERIWLLAAEPVDAAGAPGEVLPDIGLTIACGEQALAAVLV
                     QRAGRVPMSGPELLRGFPIAPGTKIG"
     gene            210..950
                     /gene="truA"
                     /locus_tag="VF547_10155"
     CDS             210..950
                     /gene="truA"
                     /locus_tag="VF547_10155"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX8263223.1"
                     /translation="MTRFRLIVEYDGGPFMGWQRQAHGPSVQQAIEEAAAAITGEEPV
                     LHAAGRTDAGVHALAMAAHLDVERPIAPFRLAEGLNARLRPLPVAILSAAAVADDWHA
                     RFSCVGRRYRYRILNRRAPPALEAGRAWQVAVPLDAGAMAQAARILVGRHDFTTFRSA
                     HCQSESPVKTLDRLDVRRDGEAVVIEAAARSFLHHQVRSMVGCLQLVGRGRWSEADLK
                     AALDARDRAALGLNAPPHGLYFVEAVYP"
     gene            complement(1081..3003)
                     /locus_tag="VF547_10160"
     CDS             complement(1081..3003)
                     /locus_tag="VF547_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HEX8263224.1"
                     /translation="MCGIAGLFYVSNPKPVDPKRVRAMTDVLAHRGPDGSGVWTAPGV
                     GLGHRRLSIIDLATGDQPMRSADGRLALSYNGEIYNFREVRAQLEAKGHRFHTESDTE
                     VVLAGWRQWGPECLERFNGMFAFALYDQDEDVLFLARDRLGVKPLYWTTLPDDALVFA
                     SELKGLLAHPLLRRAPSRGAVEDYLALGYVPDDSCMVDGVRKLAAGHYLLVRRGRVVP
                     KPVQWWDVDFSNPSGASVRSLEEELVERLREAVRSRMVADVPLGAFLSGGVDSSAVVA
                     LMAEASRSAVETCSIGFSEADHDESRWAALVADRFATSHRSRIVAADDFGLIDTLADA
                     FDEPFADASALATYRVSELARETVKVALSGDGADEAFAGYRRYRMFAAEERVRGLLPG
                     ALRGAVGAVGNLYPKLDWAPRFLRAKTTLQAIGRDSGEAYADAVGVTSQPIRSSLYTA
                     IFKRFLGGHRGERRYVRAMAEAPADDPLSRAQYADLKIWLPGDILTKVDRTSMAVGLE
                     AREPLLDHRLVEFAARLPARMRLRGGSGKWLMKRALGRYLPDEVLNRTKMGFVTPVSA
                     WFRGPLAGEAAAIAHSPALAELGWFEPAAVARLAAAHRSGRAEHGRLLWQLLMLDKSL
                     QRLFGFGAAPQVKKAG"
     gene            complement(3007..>3422)
                     /locus_tag="VF547_10165"
     CDS             complement(3007..>3422)
                     /locus_tag="VF547_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEX8263225.1"
                     /translation="AGQEVDLALAVFARQSDGRELVGYGQGASDEDGAWSWTADSAPP
                     PGGKAERIGSHGRVREVASFYRVGDVLTGSAARVKLETVRVRLLGGPQRAVAVMVSAP
                     APADGISPRPAIDAFLKDLGPIEPLADRAAGLAGP"
BASE COUNT          472 a         1258 c         1241 g          451 t
ORIGIN      
        1 gaggtggccg gcgagcggat ctggctgctc gcggccgagc cggtcgacgc ggccggcgcg
       61 cccggggagg tgctgcccga catcggcctc accatcgcct gcggcgagca ggcgcttgcg
      121 gcggtcctcg tccagcgcgc cgggcgggtg ccgatgagcg ggcccgagct gctgcgcggc
      181 ttcccgatcg ctcccggaac gaagatcggg tgacccgctt ccggctcatc gtcgaatatg
      241 acggcggccc cttcatgggc tggcagcgcc aggcgcacgg cccgtcggtc cagcaggcga
      301 tcgaggaggc ggcggcggcg atcaccggcg aggagccggt cctccacgcc gccggccgga
      361 ccgacgccgg ggtccacgcg ctcgccatgg ccgcgcatct cgacgtcgag cgcccgatcg
      421 cccccttccg gctcgccgag ggcctgaacg cgcggctgcg cccccttccc gtcgcgatcc
      481 tgtcggcggc cgcggtcgcc gacgactggc acgcccgctt cagctgcgtc ggccggcgct
      541 accgctaccg gatcctcaac cggcgggcgc cgccggcgct ggaggccggc cgggcctggc
      601 aggttgccgt cccgctcgat gccggagcga tggcgcaggc ggcgcggatc ctggtcggcc
      661 gccacgattt caccaccttc cgctccgccc attgccagtc ggagagcccg gtcaagacgc
      721 tcgaccggct cgacgtccgc cgggacggcg aggccgtcgt catcgaggcg gcggcgcggt
      781 ccttcctcca ccaccaggtg cgctcgatgg tcgggtgcct gcagctggtc gggcggggca
      841 gatggagcga agcggatctg aaggccgcgc tggacgcgcg cgaccgggcg gcgctggggc
      901 tcaacgcgcc gccgcacggg ctctatttcg tcgaggcagt ctatccctga tcctccccgt
      961 cgcggagcga tggggagggg gaccacccga agggtggtgg aggggcaatc ggccgacccc
     1021 ccctgtccca tgcttcgcag ggtccccctt cccctccgct gcgcggacgg ggaggacgaa
     1081 ttaccccgcc ttcttcactt ggggcgcggc gccgaagccg aataggcgct ggagggactt
     1141 gtcgagcatc agcaattgcc agagcagccg gccatgctcg gcgcgtccgg agcgatgggc
     1201 ggcggcgagc cgggcgacgg cggccggctc gaaccagccg agctcggcca gcgccgggct
     1261 gtgcgcgatc gccgcggcct cgccggccag cggtccccgg aaccaggcgc tgacgggggt
     1321 gacgaagccc atcttggtcc ggttcagaac ctcgtccgga agatagcggc cgagcgcccg
     1381 cttcatcagc catttgcccg agccgccgcg aagccgcatc ctcgccggca gccgagccgc
     1441 gaactcgacc aggcgatggt cgagcagcgg ctcgcgcgcc tcgaggccga cggccatgct
     1501 ggtgcggtcg accttggtga ggatgtcgcc gggcagccag atcttgaggt cggcatattg
     1561 ggcgcgggag agcggatcgt ccgccggcgc ctcggccatc gccctcacat agcgccgctc
     1621 gccgcggtgc cctcccagaa agcgcttgaa gatcgcggtg taaaggcttg atctgatggg
     1681 ctgcgacgtc acgcccaccg cgtcggcata ggcctcgccg gaatcgcggc cgatcgcctg
     1741 cagggtggtc ttggcgcgca ggaagcgggg cgcccagtcg agcttgggat agaggttgcc
     1801 gaccgccccg accgccccgc gcagcgcgcc gggaagaagg cctcggaccc gctcctcggc
     1861 ggcgaacatg cggtaccggc gataaccggc gaaggcctcg tcggcgccgt cgccggacag
     1921 ggcgaccttc accgtctcgc gcgccagctc ggagacccgg taggtggcga gcgccgaggc
     1981 gtcggcgaac ggctcgtcga acgcgtcggc gagcgtgtcg atcagcccga aatcgtccgc
     2041 ggcgacgatc cggctgcggt ggctcgtggc gaagcggtcg gcgacgaggg ccgcccatct
     2101 cgattcgtca tgatcggcct cgctgaagcc gatcgagcag gtctcgaccg ccgatcggct
     2161 ggcttcggcc atcagcgcga ccaccgccga gctgtcgacc ccgccggaga ggaaggcgcc
     2221 gagcgggacg tcggcgacca tgcgcgagcg caccgcctcg cgcagccgct cgaccagctc
     2281 ctcctccaga ctcctcacgg aagcgccgga cggattggag aaatccacgt cccaccactg
     2341 gaccggcttc ggcacgaccc gccctcggcg gaccagcaga taatggcccg cggcgagctt
     2401 cctcaccccg tcgaccatgc acgaatcgtc cgggacgtag cccagagcga gataatcctc
     2461 gacggcgccg cggctcgggg cgcggcggag cagcggatgg gcgagcaggc ccttcagctc
     2521 cgaggcgaag accagcgcgt cgtcgggcag ggtcgtccag tagagcggct tcacgccgag
     2581 ccggtcgcgg gcgaggaaga ggacgtcctc gtcctggtcg tagagggcga aggcgaacat
     2641 gccgttgaac cgctcgaggc attcggggcc ccattgccgc cagccggcca gcaccacctc
     2701 ggtgtcgctc tccgtgtgga agcggtggcc cttcgcctcg agctgcgccc tcacctcgcg
     2761 gaaattgtag atctcgccgt tgtagctcag cgccagccgg ccgtcggccg atcgcatcgg
     2821 ctggtcgccg gtggcgaggt cgatgatcga caggcgccgg tggccgagcc cgacgcccgg
     2881 cgcggtccag acgccgctgc cgtccggccc gcgatgggcg agcacgtccg tcatcgcccg
     2941 gacccgcttc gggtcgacgg gcttggggtt gctcacatag aacaggccgg cgatgccgca
     3001 catcgctcag ggccctgcca gccccgcggc ccggtcggca agcggctcga tcggcccgag
     3061 gtccttgagg aaggcgtcga tcgccgggcg cgggctgatc ccgtccgccg gcgccggcgc
     3121 cgagaccatc accgccaccg cccgctgcgg cccgccgagc agccggaccc gcaccgtctc
     3181 cagcttcacc cgcgccgcgc tgccggtcag gacgtcgccg acccggtaga agctggccac
     3241 ctcgcggacc cggccgtgcg atccgatccg ctccgccttg ccgcccggcg gcggggcgga
     3301 gtcggcggtc cacgaccagg cgccgtcctc gtcggaggcg ccctggccgt agccgaccag
     3361 ctcgcgcccg tcgctctgcc gggcgaagac ggcgagggcg agatcgacct cctggccggc
     3421 gg
//
LOCUS       DASVQZ010000357         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_365916, whole genome shotgun sequence.
ACCESSION   DASVQZ010000357 DASVQZ010000000
VERSION     DASVQZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365916"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VF547_10170"
     CDS             <1..372
                     /locus_tag="VF547_10170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8263226.1"
                     /translation="GHGGALGILYVGHGPDWVELGLDYQEKLIGVAATGVVASGPIIS
                     LMDMATSMAIWVKLGRFRHQATLDMRVDYLRPAAAGRRIHGRGECYGVTKSVGFVRGM
                     AHDGDPADPVAHVTGTFMFTG"
     gene            complement(691..1221)
                     /locus_tag="VF547_10175"
     CDS             complement(691..1221)
                     /locus_tag="VF547_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8263227.1"
                     /translation="MARQTRSNDWGFPRWRGYGTKREATQLRLCDREGCGEPGDRPAP
                     KSPHSRERWYFCEAHAAEYNRNWNYFEGVTAEEAAAREADERRGADGYRQAGHYGWGG
                     PGDGSRSRDEMRALETLEIEDGDAPFKAIKAAWRRLAKANHPDAAPGDAEAAKRFQAV
                     QAAYEVLRKAEERRTG"
     gene            complement(1327..1751)
                     /gene="rnpB"
                     /locus_tag="VF547_10180"
     ncRNA           complement(1327..1751)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF547_10180"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1758..2453)
                     /locus_tag="VF547_10185"
     CDS             complement(1758..2453)
                     /locus_tag="VF547_10185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8263228.1"
                     /translation="MDGIVDAPSPNFNERALPVSMLVLHYTGMADGPSALARLRDPEA
                     KVSAHYLVAEDGQVARLVPEEKRAWHAGRAYWRGLTDVNSASIGIEIVNPGHEFGYRP
                     FPEPQMDALVPLVADIVGRHGIRPPYVVGHSDVAPGRKQDPGELFDWHRLAKLGLAMP
                     RPTRNLIDPLWTDGGFLLALERYGYDVRQPKAAVAAFQRRFRPEHIDGEIDGETRAIL
                     LGLLLDRRSAPPR"
     gene            complement(2485..>3343)
                     /locus_tag="VF547_10190"
     CDS             complement(2485..>3343)
                     /locus_tag="VF547_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006958810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX8263229.1"
                     /translation="FLMQISDSSSRILAGLLEARTGQQLTMSRRWRIETALQPLMRER
                     DIASLDGLVAALVARGDERLADAVVEALLNNETFFFRDRQAFELLLGPALRRLETERR
                     RERRLAIWCAGCSTGQEAYSIAMHFAEQKARWSGWTIDIFASDVARGAIDQARGGVYS
                     QFEVQRGLSVMQMMRWFEEMPGAAWRIAPELRRAVRFEVGNLVGPSAPAARFDIILCR
                     NVLLYFSPEVRRAAFDRLAAAAVDDAVLMLGAGETVIGQTDAFVSDPGCRGLYLRARA
                     RPERRRAAS"
BASE COUNT          494 a         1149 c         1182 g          518 t
ORIGIN      
        1 gggcatggcg gggcgctcgg catcctctat gtcggccacg gcccggattg ggtggagctg
       61 gggctcgact atcaggagaa gctgatcggg gtcgcggcga ccggcgtcgt cgcctccggc
      121 ccgatcatca gcctgatgga catggcgacg agcatggcga tctgggtcaa gctcggccgc
      181 ttccgccatc aggcgacgct cgacatgcgg gtcgactatc ttcgccccgc ggcggcgggc
      241 aggcgaatcc acggccgagg cgaatgctac ggcgtgacga agagcgtcgg cttcgtgcgc
      301 ggcatggcgc acgacgggga cccggcggac ccggtggcgc atgtgacggg gacgttcatg
      361 ttcacggggt agcgcgccta ttcaccgccg atcattcatg accggccacc gcggcgatcc
      421 tcatacccaa cgaaatcaac cacttaagct tcggtgcctg gcactgaatt cccgccgccc
      481 cgatgcccgc ccccaaggaa atcaacggct taagcttcgg tgcctggcac cgaatttgcc
      541 gccgcggcga ttcccgtggc caacgaaatc aaggacttaa gcttcggtgc ctggcaccga
      601 atttgccgcc gcggcgattc ccgtggccaa ggaaaatcag cggcttaggg tttggtgcct
      661 ggcaccgaat tcgcaccgga tccgcggggt tcagccggtg cggcgttcct cggccttgcg
      721 gaggacctcg tacgccgcct gcaccgcctg gaagcgcttg gcggcctcgg cgtcgccggg
      781 ggcggcgtcg gggtggttgg ccttggcgag gcggcgccag gcggccttga tcgccttgaa
      841 cggggcgtcg ccgtcctcga tctcgagcgt ctccagcgcg cgcatctcgt cgcggcttcg
      901 gctgccgtcg ccggggccgc cccagccgta atggccggcc tgccggtagc cgtcggcgcc
      961 gcggcgctcg tcggcctcgc gggcggcggc ctcctcggcg gtcacgccct cgaaataatt
     1021 ccagttgcgg ttgtactcgg cggcgtgcgc ctcgcagaaa taccagcgct cccggctgtg
     1081 cggcgacttg ggcgccggcc ggtcgcccgg ctcgccgcag ccctcgcggt cgcacagccg
     1141 cagctgggtc gcctcgcgct tggtgccgta gccgcgccag cgcggaaatc cccagtcgtt
     1201 ggaacgcgtc tgtcgagcca ttgccgcctt ctaggttttg tcccgcaaag tgggaaaccg
     1261 tttcccggcg aaggccggaa tccggctggc cttcccggac ccccggtcgt cgccggggaa
     1321 cggaaaaatg ccaggggatc tataagccgg gttctgtccg ccccgtaaag ggctgtgcgg
     1381 ccattcgtct gggagacgga tcgctccgcc cctcaagcaa ccaacccgga ccgcatcggg
     1441 cggaacgagc ccgcggtccc tattcggtct tgctcccggt ggggtttgcc gtgccggccg
     1501 ccgttgccgg ggccgcggtg ggctcttacc ccaccctttc accatcgcag ggccgaagcc
     1561 tgtgcgacct gctttctgtg gcactttccc tgggccggaa atcgctctcc ggcccgccgg
     1621 acgttatccg gcaccgtcgt tccgtggagc ccggactttc ctcccgccta cgccgaggct
     1681 tcggcgggtg aaccctttcg agcttgcccg ccgtagcttc agcgaaggcg ggcggccgcc
     1741 cgatcccctg gcccgcccta tctaggcggg gcgcttcgcc gatccaagag caaaccgagc
     1801 aggatggcgc gggtctcgcc gtcgatctcc ccgtcgatat gctcggggcg gaagcggcgc
     1861 tggaaggcgg cgacggccgc cttgggctgg cggacgtcgt agccgtagcg ctcgagcgcc
     1921 agcaggaagc cgccgtcggt ccacagcgga tcgatcaggt tgcgcgtggg ccggggcatg
     1981 gcgaggccga gcttcgccag ccggtgccag tcgaacagct cgcccggatc ctgcttgcgg
     2041 cccggggcaa cgtcggaatg gccgaccaca tagggcggcc ggatgccgtg gcggccgacg
     2101 atgtcggcga ccagcggcac cagcgcgtcc atctgcggct cgggaaaggg ccgatagccg
     2161 aactcgtggc cgggattgac gatctcgatg ccgatgcttg cggaattgac gtcggtgagg
     2221 ccgcgccaat aggcccggcc ggcatgccag gcgcgcttct cctcggggac gaggcgggcc
     2281 acctgcccgt cctcggcgac gagataatgg gccgaaacct tcgcttccgg gtccctcagc
     2341 cgggcgagcg cggaagggcc gtccgccatg cccgtgtaat ggaggacgag catcgagacg
     2401 ggcagcgcgc gctcattgaa attgggcgag ggggcatcga cgatcccgtc catcggcctt
     2461 tcccgcgcca aaaaccgcgt ggcgtcaaga ggcggcccgg cgccgctccg gccgggcgcg
     2521 ggcgcgcagg tagaggccgc ggcagccggg atcggagacg aaggcgtcgg tctggccgat
     2581 cacggtctcg ccggcgccga gcatcagcac cgcgtcgtcc acggccgcgg cggcgagtcg
     2641 gtcgaaggcg gcccggcgaa cctccgggga aaaatagagg agcacgttgc ggcacaagat
     2701 gatgtcgaac ctggcggcgg gcgcggacgg gccgaccagg ttgccgacct cgaagcggac
     2761 cgcccgcctc agctcgggag cgatccgcca ggcggcgccg ggcatctcct cgaaccagcg
     2821 catcatctgc atcaccgaga ggccgcgctg gacctcgaac tgcgaataga cgccgccgcg
     2881 cgcctggtcg atggcgccgc gggcgacgtc gcttgcgaag atgtcgatcg tccagccgct
     2941 ccagcgcgcc ttctgctcgg cgaaatgcat cgcgatcgaa taggcttcct ggccggtcga
     3001 gcatcccgcg caccagatgg cgaggcggcg ctcgcggcgg cgctccgtct ccagccggcg
     3061 aagcgccggc ccgagcagaa gctcgaacgc ctggcggtcg cggaagaaga aggtctcgtt
     3121 gttgagcagc gcctccacga ccgcgtcggc gagccgctcg tcgccgcggg cgacgagggc
     3181 ggcgacgagg ccgtcgagcg aggcgatgtc gcgctcgcgc atcaagggct ggagcgccgt
     3241 ctcgatccgc cagcgccggc tcatcgtcag ctgctgcccg gttcgggcct ccagcagccc
     3301 ggcaaggatg cggctcgagc tgtcgcttat ttgcatgagg aaa
//
LOCUS       DASVQZ010000358         8647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_941868, whole genome shotgun sequence.
ACCESSION   DASVQZ010000358 DASVQZ010000000
VERSION     DASVQZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941868"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="VF547_10195"
     CDS             complement(<1..1759)
                     /locus_tag="VF547_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX8263230.1"
                     /translation="MILGLVSKLSSMGRVRKRSILIGHDLAAMAVALWAAFSTRLGEL
                     YFPDHPSVLGAAAASFLIGVIALYHLQVYHLVLRYFHMRAVSRILAASAVAAMAWVIL
                     VYFTQAKIEVDGLIMMVPRSVGFIYCGFLFLLLFMGRYAMALILSGAVHHGPPGSREG
                     QRNIAIYGANAAGISLAESMRGSRAYRLVAFVDDDRALHGQIVSGAAIYAPKALPDLI
                     AREAVDEVFLAMPKATRSQRLAAISLLTKLNLKVKTVPAPEEIVSGRFTVSDVRPIDV
                     NDLLRRDPVEPLTELIRQAVEGHSILITGAGGSIGSEVCRQIVRARPRRLVLLDHSEF
                     LLYSIELQLEEIRHGLPKGERPEIIPVIGSILNEQLVRELISEHNIDSVYHVAAYKHV
                     PLLEHNEVVGVENNVFGTLAVARAAFDARLTRFTMISTDKAVRPESVMGASKRAAELI
                     IQGLAEQEGCRTRFGIVRFGNVLDSSGSVVQRFRDQIRRGGPVTVTHKDVTRFFMSIP
                     EATQLVLQASAMAERGEVFVLDMGDPIKISELARNMITLSGMTVRDEANPLGDIEIVY
                     VGLRPGEKLYEELFVGGNSV"
     gene            complement(1794..3008)
                     /locus_tag="VF547_10200"
     CDS             complement(1794..3008)
                     /locus_tag="VF547_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX8263231.1"
                     /translation="MTALGTAETLHDVAAGSLAQTPAEHWPFYDEDEIAAVARVLRSG
                     RVNQWTGGEVFAFERALEERFQGGRGIALANGSLALELALRAFGIGPGDEVIVTPRTF
                     VASAFCAMLVGATPVFADVDPDSGNITAETIEAVLTERTRAVIPVHIAGWPAPMEPIM
                     ALARRHGLKVIEDCAQAVGAEIGGRTIGSFGDAAAFSFCQDKIISTGGEGGYVSFKDS
                     AAWQWAWSFKDHGKNRDKAFEPNPRPGFRWLHDSVGTNWRMPGPLAAIGLAQLAKLDR
                     WVETRRRNAAIWTEALAGIPGLTVPIPGADVRHASYKHYFFLDDDPDAERLRDSILAK
                     AAEVPIKVFSGTCSEVYLEKAFDGLPRPDLPVARSLGRRSLMVEVHPTLRPDLLALRA
                     EALADIVKGVLG"
     gene            complement(3037..4233)
                     /locus_tag="VF547_10205"
     CDS             complement(3037..4233)
                     /locus_tag="VF547_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263232.1"
                     /translation="MTILGFIGIILGFLAISVAFTRPTRAKVCYLTLIYLMHVAVSVV
                     YYWRAQVNVADSAMYYYDPLGTYEDGFGLGTQAIIYITQAIKTSVGGTYLDFYLLFQP
                     IGFLGICVLLRAFEEIYDELRLPHSVYLYALVAIPSLHFWSSALGKDTPFLLAVSLAL
                     WASMRLPSRAKALLAAVLLMLLIRPHVALVAVAALSLAVVVGRGIPLYLRVALFIVAA
                     AGSGVAATAIESTYAIDVTSSESIGQQFERRDNVLQSDEAGNSAVSASFPIRLLSLLF
                     RPFFFDADEPFALVASFESAFVIYIVLFMLLRWREVWRLFRSVFFVRYALIYAFGLTI
                     FLTLSYWNVGLGLRQKWSMLMPTYFVLFVAVLAVRLAKKRSAEGLVLAPSLAYPPAAA
                     QTRTAV"
     gene            complement(4289..5668)
                     /locus_tag="VF547_10210"
     CDS             complement(4289..5668)
                     /locus_tag="VF547_10210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8263233.1"
                     /translation="MNGRFWTFAASAYLVACLLLGGASAAGAAANAALQIVAVVLILF
                     WTWRRDAPLPREARGPIWIGGAFLAIGLLTLVPLPPSIWSGLPFRDQILGAYQLIGMA
                     PPAIPLSLAPPATIWSLLSLLPPAAMFLMVAGLSNEERRRLAMPLLVVACVSIVLGAF
                     QLFGGSESPLRPYAVTNPDSPVGFFANVNHEATLLLCSLPFIAFLAGRAAGRRSRSKR
                     SGGAVISIAIGLFLAVGIAISGSSAAYGLFIPAALASLLIYRRAVVGRIGWPWTAGMA
                     FVLVVFTVAALRGPLSSEAFAGDLGDQPSSRRVLAATTGRAIADSFPAGTGLGTFSTI
                     YRRYENPDRATRQYANHAHNDYLEFVLELGVAGLLLILAFIAWWAMALVRVWRSEVPG
                     AGLARAASVAIGVVLLHSLVDYPLRTAAIAAVLGLACALLIPAPRRDAEASEDAAPTG
                     SDLRHLEAV"
     gene            complement(5665..6897)
                     /locus_tag="VF547_10215"
     CDS             complement(5665..6897)
                     /locus_tag="VF547_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263234.1"
                     /translation="MALGSQGEARSSGRRAAAWAVTAASPLIVYLVVKATAAGIAPAA
                     AVALAALPPADPSPTLKVLGRVAQQQNYRVDDSTLGQARQGLTRLPLAFEPFFVAARA
                     AEQRGDLRRAIALMEEARHRRPSYPAVRMQLMVYYTKAQRFREALAEVDVILRRNGEL
                     RPVLLPELTKLIADRRGRAALAAILATEPSWREEFFNAAGARKLDPADARDLYQRVRA
                     LKPRGDLRLERQLILQAQASTGDYAGARDTLVAGLPERERAANRFLFDGAFRGVSAPK
                     PFGWKFEDLDVGRAEPSRDGGRTYLDVAYFGGRAVVLAEQVLALRPGRYTLRLAARSD
                     NGISSGTLFWRVTCLPAHNPIGVLVMSQEGPADRRFAAPVAVPATGCASQALRLVAEP
                     GDVAAVVNLQVSNLEIVQ"
     gene            complement(6900..>8647)
                     /locus_tag="VF547_10220"
     CDS             complement(6900..>8647)
                     /locus_tag="VF547_10220"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX8263235.1"
                     /translation="IAQQRDSRLIGISIESTDPELAAKLANAYADNFIQQNMERRYEA
                     TSYARNFLQQRIAAVKTKLEESERSLVEYARKQGIITLTVDGGGADTPKQEQSIDAAT
                     LVQNNNALAQARSERIAAEGRYRQAAGAQSRVAVISDPTVQILQQQRATLESQYQQKL
                     ALFKPDFPEMVQLRNQIDTLAKEIANHTRNVSGSASGALQAEYQAAVARENALAAKVE
                     QNKAALQDLRERSIQYTILQREVDTNRSLYDALLQRFKEVGVAGGVGANVVSVVDRAR
                     TPGAPFKPNLPLNIALGLFIGVLLGFGTAFAIEWIDDTIKTPDDLATKLGLASLGVIP
                     AVGKGIAVREQLEDPRSQVSEAYQSVRTALQFSTDHGVPRSLLITSTRASEGKSSTAL
                     ALAQTLASLGATVLLLDADLRKPTFRGPSSTSQGLSSLLAGSDNIAECIHPTQMERLF
                     LLPSGPIPPNPAELLASGRFKEVLDEVLAKFDHVVIDGPPVLGLADAPMLASATEGTL
                     MVIEAGAVRRAAVLNSVNRLRFAEARVMGGILTKFNATKVGYGYGYGYGYGEDVYAYR
                     EGDEPKKQIELLKSS"
BASE COUNT         1453 a         2817 c         3120 g         1257 t
ORIGIN      
        1 cgaccgaatt gccgccgacg aacagctcct cgtagagctt ctcgcccggc ctgaggccga
       61 catagacgat ctcgatatcg cccagcggat tggcctcgtc gcgcacggtc atccccgaga
      121 gggtgatcat gttgcgggcc agctccgaaa tcttgatcgg atcgcccatg tcgaggacga
      181 acacctcgcc ccgctcggcc atggcgctgg cctggaggac gagctgcgtc gcttccggga
      241 tcgacatgaa gaagcgggtg acgtccttgt gggtgacggt caccggcccg ccgcggcgga
      301 tctggtcgcg gaaccgctgg accaccgacc ccgaggaatc gagcacgttg ccgaagcgca
      361 cgattccgaa gcgggtccgg catccctcct gctcggcgag gccctggatg atcagctcgg
      421 cggcccgctt gctggcgccc atcacgctct cggggcggac ggccttgtcg gtggagatca
      481 tcgtgaagcg ggtcagcctg gcgtcgaagg cggcgcgggc gacggccagc gtgccgaaca
      541 cgttgttctc gacccccacg acctcgttat gctcgagcag cggcacatgc ttgtaggcag
      601 cgacgtggta gacgctgtcg atattgtgct cggagatcag ctcccggacc agctgctcgt
      661 tgaggatcga gccgatgacc gggatgatct cgggccgctc tcccttgggg aggccgtggc
      721 ggatctcctc gagctgcagc tcgatcgaat agagcaggaa ctcgctgtga tcgagcagca
      781 ccagccgccg cgggcgggct cggacgatct ggcggcacac ctcggacccg atcgagccgc
      841 ccgcgccggt gatcaggatc gaatggccct cgaccgcctg gcggatcagc tcggtcagcg
      901 gctcgaccgg atcgcggcgg agcaggtcgt tgacgtcgat cgggcggacg tcgctgacgg
      961 tgaagcgtcc cgacacgatc tcttcgggcg ccggcaccgt cttcaccttg aggttgagct
     1021 tggtgaggag cgagatcgcc gccagccgct gcgaacgggt cgccttcggc atcgccagga
     1081 acacctcgtc cacagcttcg cgcgcgatca ggtcgggcaa ggccttgggc gcgtagatgg
     1141 ccgcgcccga gacgatctgg ccgtgcaggg cgcggtcgtc gtcgacgaag gcgacgagcc
     1201 ggtaggcccg gcttccccgc atcgattcgg cgaggctgat gccggcggca ttggcgccgt
     1261 agatggcgat gttgcgctgg ccctcgcggg agcccggcgg gccgtgatgg acggcgccgg
     1321 agaggatcag ggccattgcg tatcggccca tgaacaggag caggaacagg aacccgcaat
     1381 agatgaaccc gaccgagcgg ggcaccatca tgatgagccc gtccacctcg atcttcgcct
     1441 gggtgaaata gaccaggatc acccatgcca tggccgcgac cgcagaggcc gccaggatcc
     1501 ggctcaccgc ccgcatgtgg aaatagcgca ggacgagatg gtagacctga agatggtaga
     1561 gcgcgatcac tccgatcagg aaggatgcgg ccgccgcccc cagcacgctg ggatggtcgg
     1621 ggaaatagag ctcgccgagc cgcgtcgaga aggccgccca caaggcgacg gccatcgcgg
     1681 ccagatcgtg gccgatcagg atggaccgct tgcgcacgcg gcccatcgac gacagtttcg
     1741 agactagtcc caggatcata ttcgcttccg cctgccgcct cgtgggcgtc cgatcatccc
     1801 aacacgcctt tgacgatatc cgcaagcgct tccgccctca gcgccagcag gtccggccga
     1861 agcgtcggat ggacttcgac catcaggctg cgccggccga gcgagcgcgc caccggcaga
     1921 tccggccggg gcaacccgtc gaaggccttc tccagataca cctccgagca ggttcccgaa
     1981 aagaccttga tcggcacctc ggcggccttg gcgagaatcg aatcgcgcag ccgctccgcg
     2041 tccgggtcgt cgtccaggaa gaaataatgc ttgtaggagg cgtggcggac gtcggcgccg
     2101 gggatcggca cggtgaggcc ggggatgccg gccagcgcct cggtccagat cgccgcgttg
     2161 cgccgccggg tctccaccca ccggtcgagc ttggcgagct gcgccaggcc gatcgcggcc
     2221 agcggccccg gcatccgcca gttggtcccg acgctgtcgt ggagccagcg gaagccgggc
     2281 cgcggattgg gttcgaaagc cttgtcgcga ttcttcccat ggtccttgaa cgaccaagcc
     2341 cattgccagg cggcgctgtc cttgaagctg acgtagccgc cctcgccgcc ggtcgagatg
     2401 atcttgtcct ggcagaagga gaaggcggcg gcgtcgccga agctgccgat cgttcggccg
     2461 cctatctccg ctcccaccgc ctgggcgcaa tcctcgatca ccttgaggcc gtggcggcgg
     2521 gcgagcgcca tgatcggctc catcggtgcc ggccagccgg cgatgtggac gggaatgacg
     2581 gctctggtgc gctcggtgag cacggcctcg atcgtctcgg cggtgatgtt gccgctgtcc
     2641 gggtcgacgt cggcgaacac cggcgtcgct ccgaccagca tcgcgcagaa ggctgaggcg
     2701 acgaaggtcc ggggggtgac gatcacctcg tcgccggggc cgatgccgaa ggcgcgaagc
     2761 gccagctcca gcgcgaggga gccgttggcg agcgcgatcc cgcggccgcc ctggaagcgc
     2821 tcctccaggg cccgctcgaa ggcgaacacc tcgcccccgg tccactggtt caccctgccc
     2881 gagcgaagga cccgagccac cgcggcgatc tcgtcctcgt cgtagaaagg ccagtgctcg
     2941 gccggcgtct gggcgagcga tccggcggcg acgtcgtgaa gggtttcggc ggttcccagt
     3001 gcagtcatgg cttcggcaat cctctgcgaa tatcgcctag acggcggtcc gggtctgcgc
     3061 ggcggccggc ggataggcga gcgaaggcgc caggaccagg ccttcggcgc ttcgcttctt
     3121 cgccagacgg acggccagca ccgccacgaa caggacgaaa taggtcggca tcagcatcga
     3181 ccatttctgt cgaaggccga gccccacgtt ccagtaggac agggtcagga agatcgtcag
     3241 cccgaacgcg tagatcaagg cgtagcgcac gaagaagacg ctccggaaca atcgccagac
     3301 ctctcgccac cgcagcagca tgaacaggac gatgtagatg acgaaggcgc tctcgaacga
     3361 ggcgaccagc gcgaacggct cgtccgcatc gaagaagaac ggccggaaga gaaggctgag
     3421 cagccggatc gggaaggacg cgctgacggc gctgttgccg gcttcgtcgg actgcagaac
     3481 gttgtcgcgg cgctcgaact gctggccgat cgattcgctg ctggtgacgt cgatggcata
     3541 ggtcgattcg atcgccgtcg cggcgactcc gctccccgcc gccgcgacga tgaacagcgc
     3601 tacccgaagg tagagcggga tgccccgccc gacgacgacc gcgagcgaca gggccgccac
     3661 cgcgaccagc gcgacgtgcg gtcgaatgag gagcatcagc agaaccgccg cgagcagcgc
     3721 cttggcgcgg ctgggcagcc gcatcgaagc ccacagcgcc agcgatacgg ccagcaggaa
     3781 gggcgtgtcc ttgccgagcg cgctgctcca gaaatggagg ctggggatgg ccaccagggc
     3841 atagagatag accgaatgcg gaagccgcag ctcgtcataa atctcttcga aagcccgaag
     3901 caggacgcat atgcccagga agccgatcgg ctggaacagc aggtagaaat cgagatatgt
     3961 cccgcccacc gaggtcttga tggcctgggt gatgtagatg atcgcctgag tgcccaggcc
     4021 gaaaccgtcc tcgtaggtgc cgagcggatc gtaataatac atcgccgagt cggcgacgtt
     4081 cacctgggcg cgccagtagt acacgacgct gacggcgacg tgcatcagat agatcagcgt
     4141 cagatagcac accttcgccc gggtcggacg ggtgaaggcc accgagatgg ccaggaagcc
     4201 gaggatgatt ccgatgaagc ccaggatggt cactggagtc ccctatgggt tgcagggcgg
     4261 cgcggccgga tcaggtcgat aggcacgatc agaccgcctc gagatggcga aggtcggatc
     4321 cggtcggcgc ggcgtcctcg gacgcttcgg cgtcccggcg cggcgccgga atcagcaggg
     4381 cgcaggcgag gccgaggacg gccgctatgg ccgcggtccg gagcggatag tcgacgaggc
     4441 tgtgaagcag gacgactccg atcgcgaccg acgcggcccg cgcgaggccg gcgccgggaa
     4501 cctcgctgcg ccacacccgc accagcgcca tcgcccacca ggcgatgaag gcgaggatca
     4561 gaagcaggcc ggccacgccg agctcgagca cgaactccag atagtcgttg tgggcgtgat
     4621 tggcatattg ccgggtcgcc cggtcgggat tctcgtagcg gcggtagatg gtcgagaagg
     4681 tgccgagccc ggttccggcc ggaaacgaat ccgcgatcgc gcggcccgtc gtcgccgcga
     4741 gcacgcgccg gctcgacggc tggtcgccga gatcgccggc aaaggcctcg ctgctgagcg
     4801 ggccgcgaag cgcggcgacg gtgaagacga ccagcacgaa ggccataccc gccgtccacg
     4861 gccagccgat ccgcccgacg acggcgcgcc ggtagatgag caggctggcc agggcggccg
     4921 gaatgaagag accataggcg gcgctcgagc cgctgatcgc gatcccgacc gccaggaata
     4981 ggccgatggc gatgctgatg accgcgcctc cgctgcgctt ggagcgcgat cgccggcccg
     5041 cggcgcgacc ggcgaggaag gcgatgaacg gcagcgagca gagcagcagg gtcgcttcgt
     5101 ggttgacgtt ggcgaagaac ccgaccggcg aatccggatt ggtcacggca taggggcgaa
     5161 gcggcgactc gctgcccccg aagagctgga aggcgccgag cacgatcgac acgcaggcca
     5221 cgacgagcag cggcatcgcc agccgccgcc gctcctcgtt ggagaggccc gcgaccatca
     5281 ggaacatcgc cgccggcggc agcagcgaaa gcagcgacca gatggtggcg ggcggcgcca
     5341 gcgaaagcgg gatcgccggc ggcgccatgc cgatcagctg ataggcgccg aggatctggt
     5401 cgcggaacgg caggcccgac cagatcgacg gcggcagcgg gacgagggtg agcaggccga
     5461 tggcgaggaa ggcgccgccg atccagatcg ggccgcgcgc ctcgcgcggc agcggcgcgt
     5521 cgcggcgcca cgtccagaac aggatcagca cgaccgcgac gatctgaagc gccgcattgg
     5581 ccgccgcccc ggcggcgctg gcgccgccga gcagcaggca ggcgacgaga taggccgagg
     5641 ccgcgaaggt ccagaagcgc ccgttcactg gacgatctcc aggttggaca cctgcaggtt
     5701 gacgaccgcg gcgacgtcgc cgggctcggc gaccagcctc agcgcctggc tggcgcagcc
     5761 cgtggccggg acggcgacgg gcgcggcgaa gcggcggtcg gcgggcccct cctgcgacat
     5821 gacgaggact ccgatcgggt tgtgcgcggg gaggcaggtc acccgccaga acagcgtgcc
     5881 cgaggagatt ccgttgtcgc tgcgcgcggc cagccggagg gtgtagcggc cgggccgaag
     5941 cgccagcacc tgctcggcca ggacgacggc ccggccgccg aaataggcga cgtcgagata
     6001 ggtccgtccg ccgtccctgg acggctcggc gcggccgacg tcgagatcct cgaacttcca
     6061 gccgaagggc ttgggcgccg acacgccgcg gaaggcgccg tcgaagagga accggttcgc
     6121 cgcgcgctcg cgctcgggca gcccggcgac gagggtgtcc ctggctccgg catagtcgcc
     6181 ggtcgaggcc tgggcctgga ggatcagctg gcgctcgagc cgaaggtctc ccctgggctt
     6241 gagcgcccgc acccgctggt agaggtcgcg cgcatcggcc gggtcgagct tgcgcgcccc
     6301 ggcggcgttg aagaactcct cgcgccacga cggctcggtg gcgagaatgg cggcgagcgc
     6361 cgcgcggccg cgccggtcag cgatcagctt ggtgagctcg gggagcagga ccgggcgcag
     6421 ctcgccgttg cggcggagga tcacgtcgac ctcggctagc gcctcgcgaa agcgctgggc
     6481 cttggtgtaa tagaccatca gctgcatccg caccgccgga taggagggcc ggcggtgccg
     6541 cgcctcctcc atcagcgcga tggcgcggcg aaggtcgccg cgctgctcgg cggcccgcgc
     6601 cgcgacgaag aagggctcga aggcgagcgg aagccgggtc agcccctgcc gcgcctggcc
     6661 gagggtcgaa tcgtcgaccc ggtaattctg ctgctgggcg acccggccga gaaccttgag
     6721 ggtcgggctc gggtcggccg ggggcagggc ggccagggcg acggccgccg ccggagcaat
     6781 tccggcggcg gtcgccttga cgacgagata gacgatcagc ggggaggcgg cggtcaccgc
     6841 ccaggcggcg gcgcgccggc cggagctgcg ggcctccccc tgtgagccga gtgccacgat
     6901 cagctgctct tcagaagctc gatttgcttc ttgggctcgt cgccttcgcg ataggcatag
     6961 acgtcctcgc catagccgta gccatagcca tagccgtagc cgaccttggt ggcgttgaac
     7021 ttggtgagga tgccgcccat cacccgcgcc tcggcgaagc gcaggcggtt gaccgagttg
     7081 agaacggcgg cgcggcgcac ggctccggcc tcgatcacca tcagcgtgcc ctcggtcgcc
     7141 gaagcgagca tcggcgcgtc ggcgaggccg agcacgggcg ggccgtcgat gacgacatgg
     7201 tcgaacttgg cgagcacctc gtcgagcacc tccttgaacc ggcccgaggc caggagctcc
     7261 gccgggttgg gcgggatcgg gccgctgggc agcaggaaca gccgctccat ctgggtcggg
     7321 tggatgcact cggcgatatt gtcggagccg gccagcaggc tggacaggcc ctggctggtc
     7381 gagctcgggc cgcggaaggt cggcttgcgc aggtcggcgt cgagcaggag gacggtcgcg
     7441 ccgaggctgg cgagcgtctg ggcgagcgcg agggcggtgc tcgacttgcc ctccgacgcc
     7501 cgggtgctgg tgatcagcag gctgcgcggg actccgtggt cggtcgagaa ttgcagcgcc
     7561 gtcctgaccg actgataggc ttccgagacc tggcttcgcg gatcctcgag ctgctcgcgc
     7621 acggcgatgc ccttgccgac ggccgggatc accccgagcg aggcgaggcc gagcttggtg
     7681 gcgaggtcgt cgggcgtctt gatcgtgtcg tcgatccact cgatcgcgaa ggcggtgccg
     7741 aagccgagca ggactccgat gaacaggccg agcgcgatgt tgagcggcag attgggcttg
     7801 aacggcgccc ccggcgtgcg ggcccggtcg acgaccgaga cgacgttggc gccgactccg
     7861 cccgccacgc ccacttcctt gaagcgctgg aggagcgcat cgtagaggct tcggttggtg
     7921 tcaacctcgc gctgcaggat cgtgtactgg atgctgcgct cgcgcaggtc ctgcagggcg
     7981 gccttgttct gctcgacctt cgcggcaagc gcgttctccc gcgcgaccgc ggcctgatat
     8041 tccgcctgga gcgccccgga ggccgagccg gagacgttgc gggtgtggtt ggcgatttcc
     8101 ttggcgagcg tgtcgatctg gttgcgaagc tggaccatct ccgggaagtc cggtttgaaa
     8161 agggcgagct tctgctgata ttgcgattcg agcgtggcgc gctgttgctg aaggatctgg
     8221 accgtcgggt cgctgatcac cgccactcgc gactgggcgc cggcggcctg ccgatagcgt
     8281 ccctcggcgg cgatacgctc acttcgcgcc tgcgccagag cgttgttgtt ctggaccagg
     8341 gtggcggcgt cgatcgactg ctcctgcttg ggcgtgtcgg ccccgccgcc gtcgacggtc
     8401 agcgttatga tcccctgctt gcgcgcatat tcgaccagcg accgctcgga ttcctcgagc
     8461 ttggtcttca ccgcggcgat gcgctgctgg aggaagttgc gcgcgtagga ggtcgcctcg
     8521 tagcggcgct ccatgttctg ctggatgaaa ttgtcggcat aggcgttggc gagcttggcg
     8581 gcgagctcgg gatcggtgct ctcgatgctg atcccaatca gccggctgtc gcgctgctgg
     8641 gcgatgt
//
LOCUS       DASVQZ010000359         5656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_845601, whole genome shotgun sequence.
ACCESSION   DASVQZ010000359 DASVQZ010000000
VERSION     DASVQZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845601"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VF547_10225"
     CDS             complement(<1..227)
                     /locus_tag="VF547_10225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8263236.1"
                     /translation="MTAIPPLAVVMPVRNTLPYLDECIASILTQGFGDFEFAIYDDAS
                     DDGSGERLRHWAARDARIRLVRGETRLGPTG"
     gene            complement(224..1249)
                     /locus_tag="VF547_10230"
     CDS             complement(224..1249)
                     /locus_tag="VF547_10230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8263237.1"
                     /translation="MSTPPLSVAMPVRDALPYLDESIASILGQSFADFEFTIYDDSSV
                     DGSSERLRQWAARDSRIRLVRGEAPLGPARSAQSAVEMSRAPLIARMDADDISNPGRL
                     GRQLELFRNHPDAVLAAALSEGIDGAGRSVRPVDAWRLARNAPFAPFAHSSIMFRRDA
                     FMAVGGYRAEADYWEDLDFLYRMAGRGRLLVVPETLSKVRHSRSSARLREECDRVENA
                     VDTMYRAAAAFRAGEDYEPLLRRRRAPGEKLDPRTFVGRGSTLLWSGERPRMLGRLLR
                     RGDLGANVSSLNALVWGVVGELSPHALRLAIRTALRLGNLAARRRLAGRDAVEWDPRA
                     GSGKDRA"
     gene            complement(1246..1965)
                     /locus_tag="VF547_10235"
     CDS             complement(1246..1965)
                     /locus_tag="VF547_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263238.1"
                     /translation="MERACLRSVLRQGHRLTLYCYEPPSGVPDGVELADAGRILPRSR
                     VIRHRGGSPSLFSNLFRYELQRRGLGTWVDADVYLLAPLDGAGDYLLGQEAPGVINGA
                     VLRLPPDSPLLPPLIALFDEGEVPSWLPWRPRLAARLRLAVRGRTGLADMPWGSAGPK
                     ALSFLAARFGVADRALPSEIFYPVPWQEADWIVDPAESLDQKAGPRTVAVHLWNERIK
                     SVKDAPAPPGSFLARLQAEGA"
     gene            2166..3869
                     /locus_tag="VF547_10240"
     CDS             2166..3869
                     /locus_tag="VF547_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8263239.1"
                     /translation="MDAFLALFRLLSPARRRQFALTLLLMLAGAVAEMVTIGAALPFL
                     ALVADPQSALVPPRFLRLLETVGGSAVVGAALLLIVAALASAMVRLALTWGSQRFVMA
                     AGHDMAAAVFGRMLRQPYAEQVRRNSSTTLAAIDKVQGVVFGLLQPAMQGLIGVFIAL
                     CVFAVLLRIDARAAGMAAVSVTLAYVAVSLLVRPRLRRNSKALAGTMVDRTRTIQEGL
                     GGIRDIILDRSEPLFEAKFRELDSRYRRAQVGTQFVAGAPRFVVEAAGLVAIAVVTLA
                     MSLEPGGVVKAIPVLGALALGAQRLLPLLQQAYYGWSLASGNFQAFRDVIEFMQAPVP
                     EPQPVPARPLAFERELVFDRVGFRYPEGRFGLVDVDLRIRAGEHVGIAGPTGGGKSTL
                     LDLLMGLLEPDRGEIRVDGRPLDAASRAAWQAGLAHVPQTIYLADDSIAANIAFPRAA
                     EGMDPERLDAAVRAARLDAFLAGLPEGLRTRVGERGVRLSGGQRQRIGLARALYRRPR
                     LLILDEATSALDEATEAAVLAALQELRKDLTLVTVAHRATTLAACDRLIRVEGGRVSE
                     A"
     gene            3934..>5656
                     /locus_tag="VF547_10245"
     CDS             3934..>5656
                     /locus_tag="VF547_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX8263240.1"
                     /translation="MTATLPQLTAFGLLAGMMLLFIWGRIRYDLVAVLALLAALVLGL
                     VKPEDAFTGFADDIVIIVASALVISAAVERSGVVEAAIARLSGGMSRMGSQLTVLVGS
                     VTLLSALVKNIGALAMLMPAAFRMAKRSDSSPSCFLMPMAFGSLLGGLMTLVGTSPNI
                     IVSRVREDMTGEPFSMFDYTPVGAGIAAAGLLFLRFGYRLLPSDRRAAPTMGEALDIE
                     DYATEAEVPPGSAAEDKTVAELRELLERGVELTGIHRWDRRATLPLPDAVIRAGDVLI
                     LKGEPEALERVIASAGLELEGHHRDAAAGTASSNVGVIEAVIGRDSVLAGAAAGRMAL
                     HDRHGVNLIAVSRRGERITRRLRDIILCPGDVVVLQGPLDTLPERLRELGCLPLAERS
                     LRLGNARRGLIPLFVLGAAMTLTALGWVPVAIAFFGAAALIMLSGALPAREAYSRIEW
                     PILVMIGALIPVSDSLRTTGATELMAGWLSGVAATLPAWGAVALIMIAAMAVTPFLNN
                     AATVLVMAPIAASFAGGLDYRPEAFLMAVAVGAGSDFLTPIGHQCNTLVMGPGGYRFG
                     DYARLGAP"
BASE COUNT          731 a         2045 c         2060 g          820 t
ORIGIN      
        1 ctgcccgtcg gcccaagccg cgtttcgccg cggacgaggc ggatccgggc atcgcgcgcc
       61 gcccaatgcc gcaaccgctc gccggagccg tcgtcggagg cgtcgtcgta gatggcgaac
      121 tcgaagtcgc cgaagccctg ggtcaggatg ctcgcgatgc attcgtcgag ataggggagg
      181 gtgttgcgca ccggcatgac gacggcgagc ggggggatgg cggtcacgcg cgatccttcc
      241 cgcttccggc gcgcggatcc cattccacgg cgtcgcgccc cgccagccga cgcctcgcgg
      301 cgagattgcc gaggcggagc gcggtgcgga tggccagccg cagcgcgtgc gggctcagct
      361 ccccgacgac gccccagacc aaggcgttga gcgacgaaac attcgctccc agatcgccgc
      421 gccgaagcag gcggcccagc atccgaggcc gttcccccga ccagagcagg gtcgatcccc
      481 gtcccacgaa ggtgcgcgga tcaagcttct cgccgggtgc gcggcgccgg cgcagcagcg
      541 gctcgtaatc ctcacccgcg cgaaaggccg cggcggcgcg gtacatcgtg tcgaccgcgt
      601 tctcgacccg gtcgcattcc tcgcgcagcc tcgcgctgga tcgcgaatgg cgcaccttcg
      661 acagggtttc ggggacgacg agcagccgtc cgcggccggc catccggtag aggaagtcga
      721 ggtcctccca ataatcggcc tcggcgcggt agccgccgac cgccatgaag gcgtcgcgcc
      781 ggaacatgat cgagctgtgg gcgaaggggg cgaaaggcgc gttccgcgcc aggcgccagg
      841 cgtcgaccgg cctgaccgac cgtcccgccc cgtcgatgcc ttcgctcaag gcggccgcga
      901 ggaccgcgtc ggggtggttg cggaacagct ccagctggcg gccgagccgc ccgggattcg
      961 agatgtcgtc ggcgtccatc cgcgcgatca acggcgctcg gctcatctcg acggccgact
     1021 gggcgctgcg cgcagggccc agcggcgctt cgccgcggac gagccggatg cggctgtcgc
     1081 gcgccgccca ttgccgaagc cgctcgctcg aaccgtcgac ggaggagtcg tcgtagatcg
     1141 tgaactcgaa gtcggcgaag ctctggccga ggatgctggc gatgctctcg tcgagatagg
     1201 gcagggcgtc gcggacgggc atggcgacgc tgaggggcgg cgtgctcatg ccccctccgc
     1261 ctgcaggcgc gcgaggaagc tccccggagg ggccggggcg tccttcaccg atttgatgcg
     1321 ctcgttccac agatggacgg cgacggttcg cggccccgcc ttctgatcga gcgactcggc
     1381 cggatcgacg atccaatcgg cctcctgcca cggcaccgga tagaatatct cgctgggcag
     1441 ggcgcggtcc gcgaccccga accgggccgc caggaagctc agcgccttcg ggccggcgct
     1501 gccccacggc atgtcggcca gcccggtccg gccgcggacg gcgaggcgca gccgggcggc
     1561 gagccgcggc cgccagggca gccaggaggg cacctcccct tcgtcgaaca gggcgatgag
     1621 cgggggcagg agcggcgagt ccggcggcag cctgagcacc gcgccgttga tcacgccggg
     1681 cgcctcctgg ccgagcagat agtcgccggc gccgtcgagc ggggcgagca ggtagacgtc
     1741 cgcgtcgacc caggtgccca ggccgcgccg ctgcagctcg tagcggaaca ggttggagaa
     1801 gagcgaaggg ctgccgccgc gatggcgaat cacccgcgag cgcggcagga tccgccccgc
     1861 gtcggcgagc tcgaccccgt cgggcacgcc gctcggcggc tcgtagcaat agagggtgag
     1921 gcgatggccc tggcggagca cagagcgaag gcaggcccgc tccaccaacc ccagccgcgg
     1981 tcctatccaa agcgagacgc agggggggaa caccgagaga ctccgggcgg caggacctga
     2041 cggggcgagg gcttcgccgg gcggcggctg tcgcacgatc cgccccgccg ggcaaggcgg
     2101 cagggggtgc aatcgtcggc ggccccgcct aggaagcggg ctttcggagg aggcaagccc
     2161 ggcagatgga cgcgtttctc gccctgttcc ggctgctctc gccggcccgg cggcggcagt
     2221 tcgcgctgac cctgctgctg atgctggcgg gcgcggtggc ggagatggtg acgatcgggg
     2281 cggcgctccc gttcctcgcg ctcgtcgccg atccgcagag cgcgctcgtc ccgccgcggt
     2341 tcctgcggct gctcgagacg gtggggggct cggcggtggt cggagccgcg ctgctgctga
     2401 tcgtcgccgc gctcgcttcc gccatggtcc ggctcgcgct cacctgggga agccagcgct
     2461 tcgtcatggc ggccgggcac gacatggcgg cggcggtgtt cggacggatg ctccgccagc
     2521 cctatgccga gcaggtgcgg cgcaactcca gcacgaccct ggcggcgatc gacaaggtcc
     2581 agggggtcgt gttcggcctt ctccagccgg cgatgcaggg cctgatcgga gtgttcatcg
     2641 ccctgtgcgt cttcgccgtg ctgctgcgca tcgacgcgcg cgcggcgggc atggccgcgg
     2701 tgtcggtgac ccttgcctat gtcgccgtca gcctgctcgt ccggccgagg ctcaggcgga
     2761 actccaaggc gctggccgga acgatggtcg accgcacgcg gaccatccag gagggcctgg
     2821 gcggcataag ggacatcatc ctcgaccgct ccgagccgct gttcgaggcc aagttccgcg
     2881 agctcgacag ccgctaccgc cgcgcccagg tggggacgca gttcgtggcg ggggcgccgc
     2941 gcttcgtcgt cgaggccgcg gggctggtgg cgatcgcggt ggtgaccctg gcgatgagcc
     3001 tcgagcccgg cggagtcgtc aaggcgatcc cggtgctggg cgcgctcgcg ctcggcgcgc
     3061 agcggctgct gccgctcctc cagcaggcct attacggctg gagcctggcg agcggcaact
     3121 tccaggcgtt ccgcgacgtg atcgagttca tgcaggcgcc cgtcccggag ccgcagccgg
     3181 tgccggcgcg gccgctggcc ttcgagcgcg agctcgtctt cgaccgggtc ggcttccgct
     3241 accccgaggg gcggttcggg ctggtcgacg tcgatcttcg gatccgcgcc ggcgagcatg
     3301 tcgggatcgc cggcccgacc ggcggcggca agtcgaccct gctcgacctg ctgatgggcc
     3361 tgctcgagcc cgaccggggc gagatccggg tcgacggccg gccgctcgat gcggccagcc
     3421 gagccgcctg gcaggcgggc ctcgcccacg tcccccagac catctacctc gccgacgatt
     3481 cgatcgccgc caacatcgcc ttcccgcgcg ccgccgaggg gatggatccg gaacggctcg
     3541 acgcggcggt ccgggccgcc cggctcgacg ccttcctggc cgggcttccg gaagggctcc
     3601 ggacccgtgt cggggagcgc ggagtcaggc tgtcgggcgg ccagcgccag cgcatcggcc
     3661 tcgcccgcgc cctctatcgc cgcccgcgcc tgctgatcct cgacgaggcg accagcgcgc
     3721 tcgacgaggc caccgaggcg gccgtcctcg ccgcgctgca ggagctgcgc aaggacctga
     3781 ccctggtgac ggtggcgcac cgggccacca cgctggccgc ctgcgaccgg ctgatccggg
     3841 tcgagggcgg gcgggtgagc gaagcctgat ccgcctcccc gccgagcgac ggggaggcaa
     3901 ccggggcccc cgtgcggcgt tccgcccgaa cctgtgaccg cgacccttcc ccagctcacc
     3961 gccttcggcc tgctcgccgg aatgatgctg ctcttcatct ggggacgcat ccgctacgac
     4021 ctcgtcgccg tcctcgcgct gctcgccgcc ctggtgctgg ggctggtgaa gccggaggac
     4081 gccttcaccg gcttcgcgga cgacatcgtg atcatcgtcg cctccgcgct cgtcatctcg
     4141 gcggcggtcg agcgatcggg ggtggtcgag gcggcgatcg cgcgcctcag cggaggcatg
     4201 agccggatgg gttcgcagct caccgtgctg gtcggctcgg tcaccctgct ctcggccctg
     4261 gtcaagaata tcggcgccct cgccatgctg atgccggccg ccttccggat ggccaagagg
     4321 tccgacagct cgccctcctg cttcctgatg ccgatggcct tcggctcgct tctcggcggg
     4381 ttgatgaccc tggtgggcac ctcgcccaac atcatcgtca gccgggtccg cgaggacatg
     4441 accggcgagc ccttctcgat gttcgactac acgcccgtcg gcgccggcat cgccgccgcc
     4501 gggctgctgt tcctgcgctt cggctaccgg ctgcttccgt ccgaccgccg ggcggcgccg
     4561 acgatgggcg aggcgctcga catcgaggat tatgccaccg aggccgaggt cccgcccggc
     4621 tcggcggccg aggacaagac cgtggccgag cttcgcgagc tgctcgagcg cggcgtcgag
     4681 ctgaccggga tccaccgctg ggaccggcgg gcgacgcttc cgcttccgga cgccgtcatc
     4741 cgcgccggcg acgtgctcat cctcaagggc gagcccgagg cgctcgagcg cgtcatcgcc
     4801 tccgccggcc tggagctcga gggtcatcat cgcgacgccg ccgccggcac cgcctcgtcc
     4861 aacgtcggag tgatcgaggc ggtgatcggc cgcgattcgg tcctggccgg cgccgcggcc
     4921 gggcgcatgg cgctgcacga ccgccacgga gtgaatctga tcgcggtctc gcgccgcggc
     4981 gagcggatca cccggcgcct gcgcgacatc atcctctgcc cgggcgacgt cgtcgtcctg
     5041 caggggccgc tcgacaccct gcccgagcgg ctccgggagc tcggctgcct gcctctggcc
     5101 gagcgcagcc tgaggctcgg caacgcccgc cgcgggctga tcccgctgtt cgtcctcggc
     5161 gccgccatga ccctgacggc gctgggctgg gtgccggtcg ccatcgcctt cttcggcgcg
     5221 gccgctctga tcatgctctc cggggcgctg ccggcgcgcg aggcctacag ccggatcgaa
     5281 tggccaatct tggtcatgat cggggccctc atcccggtgt cggattcgct tcgcaccacc
     5341 ggtgcgaccg agctgatggc cggctggctg tccggggtcg ccgcgacctt gcccgcgtgg
     5401 ggcgccgtgg ccctgatcat gatcgcggcg atggcggtca ccccgttcct caacaatgcc
     5461 gccacggtcc tggtcatggc gccgatcgcc gcgagcttcg ccggcggcct cgattaccgg
     5521 ccggaagcct tcctgatggc ggtcgcggtc ggcgcgggat cggatttcct gactccgatc
     5581 ggccaccagt gcaacactct ggtcatgggg cccgggggct atcgcttcgg cgactatgcc
     5641 cggctcggag cgccgc
//
LOCUS       DASVQZ010000360         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_75758, whole genome shotgun sequence.
ACCESSION   DASVQZ010000360 DASVQZ010000000
VERSION     DASVQZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75758"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VF547_10250"
     CDS             complement(<1..476)
                     /locus_tag="VF547_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX8263241.1"
                     /translation="MAELLFLAHRIPFPPDRGDKIRSFHALRHLAGLGRVHLACFADD
                     EADAANLAGLRAALGPALGEAHVEVRRRSRLAAGALALLDGRPVSLALFDSPALRGFV
                     ERQLAGGRIGTVFASSGQMAQFVPAGTAARFVMDFLDVDSAKFEAYAEEGGLLAPV"
     gene            complement(476..1546)
                     /locus_tag="VF547_10255"
     CDS             complement(476..1546)
                     /locus_tag="VF547_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEX8263242.1"
                     /translation="MNLPLLKPQLRVRAEDLGDPDARRRLDAFVAGQSGSEFFHRPHW
                     SRAVERGCGQKSHCLVAERADGALAGCLPLTEIRSFLFGHALVSAGFGTGGGILAAEP
                     AAVAALAEAAWDLARSLGCGSVELRGGALPEGWPRREGVHAAFSAALQPDPEAMLRAM
                     PKRLRYEVRRALACDFEVSAGNDPRHVEAFYRVYAECVRNLGTPVFPAGLFEAMADEF
                     GRDCDVLVVWKDGRPLAANFNLYHAGAGQCHWGGGTAEARGNRANDLMMYAYMRHLAE
                     RGCTRADFGRSKIGSGAYDRKRMWHLDERPLVYAVRTAEGAAPRDVSPVSSKYRLQVE
                     LWKKLPLALANAVGPVIARGLG"
     gene            complement(1543..>2280)
                     /locus_tag="VF547_10260"
     CDS             complement(1543..>2280)
                     /locus_tag="VF547_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HEX8263243.1"
                     /translation="NGAAVLDLFAEAGVKATFFTLGWVAERHPGLIRRAAEAGHEIAS
                     HGWDHQRVFKLGEDGFRADLRRAHAAIAEACGVEPAGYRAPSFSIDARTPRAHRVLAE
                     EGYSYSSSVAPVAHDHYGWRDSPRFAHRPVPGRDLIELPVTTVELGGRRMAAGGGGFF
                     RLLPYRFSSWAIGRVNCADRRPAVFYFHPWEIDPDQPRVAGAPLKSRLRHYSRLSAMR
                     PKLLRLLKSYAWGRTDQVVERERERLS"
BASE COUNT          337 a          808 c          863 g          272 t
ORIGIN      
        1 tggaccggcg cgagcaggcc cccttcctcg gcataggcct cgaacttggc cgaatcgacg
       61 tcgaggaagt ccatcacgaa gcgcgccgcg gtcccggccg ggacgaactg cgccatctgc
      121 cccgacgagg cgaagacggt cccgatccgc ccgcccgcca gctgccgctc gacgaagccg
      181 cgcagcgccg ggctgtcgaa cagggcgagc gagaccgggc ggccgtcgag cagcgccagc
      241 gcgccggcgg cgagcctgga gcggcggcgg acctcgacat gggcctcgcc gagcgccggg
      301 ccgagcgccg cgcgcaggcc ggcgaggttc gccgcatcgg cctcgtcgtc ggcgaagcag
      361 gcgagatgga cccggccgag gccggccagg tgcctcaagg cgtggaacga gcggatcttg
      421 tcgccccggt cgggcgggaa ggggatgcgg tgggcgagga agagcagctc ggccatcagc
      481 cgagcccgcg ggcgatgacc ggcccgacgg cgttggccag cgccaagggc agcttcttcc
      541 acagctcgac ctggagccgg tacttcgagc tgaccgggct gacgtcgcgc ggcgcggcgc
      601 cttcggcggt gcggaccgca tagacgaggg gacgctcgtc cagatgccac atcctcttgc
      661 ggtcgtaggc gcccgagccg atcttggagc ggccgaagtc ggcgcgggtg cagccccgct
      721 cggccagatg gcgcatgtag gcgtacatca tcaggtcgtt ggcgcggttg ccgcgcgcct
      781 cggcggtgcc tccgccccag tggcactggc cggcgcccgc gtgatagagg ttgaaattgg
      841 cggcgagcgg gcgaccgtcc ttccacacga cgaggacgtc gcagtcgcgg ccgaactcgt
      901 cggccatcgc ctcgaacagg ccggccggga agaccggcgt gccgaggttg cggacgcatt
      961 cggcatagac tcggtagaag gcctcgacat ggcgcgggtc gttgccggcg ctgacctcga
     1021 aatcgcaggc cagcgctcgg cgcacctcgt agcgcagccg cttgggcatg gcccgcagca
     1081 tcgcctcggg atcgggctgg agcgcggccg agaaggcggc atggactccc tcgcgcctcg
     1141 gccagccttc gggcagggcg ccgccgcgaa gctcgaccga gccgcagccg agcgaccgcg
     1201 ccaggtccca ggcggcctcg gcgagcgcgg cgacggcggc cggctcggcg gcgaggattc
     1261 cgccgccggt gccgaagccg gccgagacca gggcatggcc gaacaggaag gagcggatct
     1321 cggtcagcgg caggcagccg gcgagcgccc cgtcggcccg ctcggcgacc aggcaatggc
     1381 tcttctggcc gcagccgcgc tcgaccgccc ggctccagtg cgggcggtgg aagaactcgg
     1441 agccgctctg cccggcgacg aaggcgtcga gccggcggcg ggcgtccgga tcgcccaggt
     1501 cctcggcccg cacgcgcagc tgcggcttga gcagaggcag gttcacgaca gccgctcccg
     1561 ctcgcgctcg accacctggt cggtccgccc ccaggcatac gacttcagca atctcagcag
     1621 cttgggccgc atcgcggaga gtcggctgta atggcgaagg cgcgacttga gcggcgctcc
     1681 cgccacccgc ggctggtccg ggtcgatctc ccagggatgg aaatagaaga ccgccgggcg
     1741 ccggtcggcg caattgaccc ggccgatcgc ccagctcgag aatcggtagg ggagcagccg
     1801 gaagaagccc cctccccccg ccgccatccg gcgcccgccg agctcgaccg tggtgaccgg
     1861 aagctcgatc aggtcgcggc cgggcaccgg ccggtgggcg aagcgcggcg agtcgcgcca
     1921 gccgtaatgg tcgtgggcga ccggagcgac gctggaggag taggaatagc cctcctcggc
     1981 gagcacccgg tgcgccctgg gcgtgcgcgc gtcgatcgag aagctcggcg cccgatagcc
     2041 ggccggctcg actccgcagg cctcggcgat ggcggcgtgg gcgcgcctca ggtcggctcg
     2101 gaagccgtcc tcgccgagct tgaagacgcg ctgatggtcc cagccgtggc ttgcgatctc
     2161 gtgcccggcc tcggcggcgc ggcggatcag gcccggatgg cgctcggcga cccagcccag
     2221 ggtgaagaag gtcgccttga cccccgcctc ggcgaacagg tcgagcacgg cggcgccgtt
//
LOCUS       DASVQZ010000361         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_752517, whole genome shotgun sequence.
ACCESSION   DASVQZ010000361 DASVQZ010000000
VERSION     DASVQZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752517"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1114
                     /gene="metK"
                     /locus_tag="VF547_10265"
                     /pseudo
     CDS             104..1114
                     /gene="metK"
                     /locus_tag="VF547_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            1201..>1998
                     /locus_tag="VF547_10270"
     CDS             1201..>1998
                     /locus_tag="VF547_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263244.1"
                     /translation="MGLIEKSVVLAAALVLTVPVQAGAQGPQEIRLPAKAGFKHRHSK
                     IGLPPVLAGLPRTKAAEFEADQLDMNSEYATPDAGEAYTVYIYRNVAGGLPVWFDRAR
                     TMIERRDTLGTATFHSAGPFVPPGRTNAAGLLATYALAGKGYRSTGVALVPAGEWLVK
                     LRVSSETHSPAELESRMKAALAEMDWPGKMAPAPAAALVEPCSTALSLSGDAKPAAKD
                     ENSMAESLMGALVGMAAAHQQPKQAAAAPAPEPRWCRDSIELAEGGVY"
BASE COUNT          343 a          704 c          666 g          285 t
ORIGIN      
        1 gtctcgaccc agcacgccaa gggctatgac cagggcgagc aggaagccga gctgcacgct
       61 tatgtgaagc gtgtcgtcgc cgacgtcatc ccgggcgagc tgctgggcgc cgggcgtgcg
      121 ggacgagatc gagcagacgg ttcgccaggt cgtgcagaag atcggctatg agcaggacgg
      181 cttccactgg cagacggccg acttcgagaa ccacctgcac ggccagtcgg cgcacatcgc
      241 tcagggcgtc gatgcctcgg gcaacaagga cgaaggcgcc ggcgaccagg gcatcatgtt
      301 cggcttcgcc tgcgacgaga ctccggacct gatgccggcc accctctatt acagccacaa
      361 gatcctcgag cgcatggctg ccgaccgcca tggcggcgcc gcgtccttcc tcgagccgga
      421 cgccaagagc caggtcacgc tccgcttcga gaatggcgag ccggtcgcgg cgaccgcgat
      481 cgtcgtctcc acccagcacg gcaagggcta cgaccagggc gagaaggaag cggagctcaa
      541 gagctacgtc cgccaggtcg tcgccgatgt ccttcccgcc gacctgctct ccgacgagac
      601 cgtctaccat atcaacccga ccggaagctt cgagatcggc gggcccgacg gcgacgccgg
      661 cctcaccggc cgaaagatca tcgtcgacac ctatggcggc gccgccccgc acggcggcgg
      721 cgccttctcg ggcaaggacc cgaccaaggt cgaccgttcg gccgcctata tcgcgcgcta
      781 tctggccaaa aatatcgtcg ccgccggcct cgcccgccgc tgcacgatcc agctcgccta
      841 cgcgatcggg gtctcggcgc cgctgtcgct ctacgtcgac actcacggca ccgggaacgg
      901 ggcggacgac gccgcgatcg agcgggcgat catgtccatc caggagcttg gcggcctcac
      961 cccgcgcgcc atccgcacgc atctcggact caacaagccc atctaccggc gcaccgccgc
     1021 ctacggccat ttcggccgca aggccgaagg cgacttcttc ccctgggaga agaccgacct
     1081 ggcggagaag ctgaaggcgg cgctccaggg ctgagccgga ccgccggccc cggccgtcgg
     1141 attccaccct atgcaattcg gcgcgccctg ttaggctcgc tcgaaacatg ggggggtgag
     1201 atgggactca ttgagaaatc ggtcgttctg gcggcggcgc tggtcctgac ggttccggtg
     1261 caggccgggg cgcaggggcc tcaggagata cggcttcccg ccaaggccgg gttcaagcat
     1321 cgtcattcca agatcgggct gccgccggtc ctcgccggcc ttccccgaac caaggcggcg
     1381 gagttcgaag ccgaccagct cgacatgaat tccgaatatg ccactccgga cgccggcgag
     1441 gcctacaccg tctacatcta ccgaaacgtc gccggcggac ttccggtctg gttcgaccgg
     1501 gcccggacga tgatcgagcg gcgcgacacg ctcggaaccg cgaccttcca ttccgccggg
     1561 ccgttcgttc ctccggggcg gaccaacgcc gcggggctgc tcgccaccta cgctctcgcc
     1621 ggaaaaggct atcgaagcac gggcgtggcg ctcgtcccgg ccggcgaatg gctggtgaag
     1681 ctccgggtct cctcggaaac ccattcgcct gcggagctcg aatcccgcat gaaggcggcg
     1741 cttgccgaga tggactggcc gggcaagatg gctccggcgc ccgcggccgc gctggtcgag
     1801 ccctgctcaa ccgctctctc gctgtcgggg gacgccaagc cggcggcgaa ggacgagaac
     1861 agcatggccg aatcgctgat gggagccctg gtcggcatgg cggcggctca ccagcagccg
     1921 aagcaggcgg cggcggcgcc ggcgccggag ccccgctggt gccgcgattc gatcgagctg
     1981 gccgagggag gcgtctat
//
LOCUS       DASVQZ010000362         4564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_942758, whole genome shotgun sequence.
ACCESSION   DASVQZ010000362 DASVQZ010000000
VERSION     DASVQZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942758"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VF547_10275"
     CDS             complement(<1..411)
                     /locus_tag="VF547_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8263245.1"
                     /translation="MADIGLDFALGEMADAIRDTTRRFAADRIAPLAARIDAEDWFPK
                     ELWPEMGELGLHGITVEEDYGGLGLGYLEHVVAQEEVARASASIGLSYGAHSNLCVNQ
                     IRRWASPEQKARYLPKLISGEHVGSLAMSEAGAGS"
     gene            588..1778
                     /locus_tag="VF547_10280"
     CDS             588..1778
                     /locus_tag="VF547_10280"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8263246.1"
                     /translation="MTATDPIVILSYARTPMGSFQGALAGASATELGAAAVRAAVERA
                     GVAGEAVDKIFMGCVLPAGLGQAPARQAALGAGLPRSVEATTINKVCGSGMQAAMFAH
                     DMIAAGSADLIVAGGMESMSNSPYLLKKHRSGARIGHDTVYDHMMLDGLEDAYDKGRA
                     MGTLAEDSAREYQFTRGDQDEYAIESLRRANEAIRSGAFDRETTPVTVKGRKGDEIVE
                     RDEQPGKSNPEKIPGLKPAFAKDGTITAANASSISDGAAALVIARQSVADRLGVRPVA
                     RIVAHAAFAGPPDQFTTAPVPAVRKALERAGWSVGDVDLYEVNEAFACVAMIAMRDLG
                     IPRDALNVHGGATALGHPIGASGARILTTLISALETHGQKRGLATLCIGGGEATAMAV
                     ELAD"
     gene            1792..2790
                     /locus_tag="VF547_10285"
     CDS             1792..2790
                     /locus_tag="VF547_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX8263247.1"
                     /translation="MLGKRKLQVFVSSTYSDLIEERQAAVSAILKAGHIPAGMELFAS
                     GDRSQMDVIKRWIDESDVYMVILGSRYGSVEATTGLSYTELEYDYAVNSGKPFFALVL
                     TDSAMDEKVKKHGIAVTEQDHPDKLKAFRSKVMSRMCALCQDNKDIRGGVYDSILEIL
                     QTRDLPGWVRGDSVEDPRPLHDQIALLKSEIQRLSAELDSARVATPASAASTGSAREF
                     ATLEKVLRSKKLEAPAEVAGSDEDVSTSAYSLLMTNRNLLVRGWVMAVNPEGKSLWFR
                     NNLMPILQVHRLITREDMGPKYHRYTMTELGLDLLAWFDHRAATPAKPADKSKPSK"
     gene            complement(2838..>4564)
                     /locus_tag="VF547_10290"
     CDS             complement(2838..>4564)
                     /locus_tag="VF547_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8263248.1"
                     /translation="TSVQSFEQYGTEGVYPAFQLGGTAGGRTGDLAWFAGASHVSSRS
                     HPLAYATVARPAAAGSAGTRVTGAYADVNRTGAPIFVLGAAGLEDHEQDNLKLKLAWE
                     PGPGLRLSYRGGLFLNDTEASAETYLSGNAYAGTLNIDGRTVSVPASAFSNNVYTLDQ
                     RHWMHGLAAEGEAAGLRWRAVGSLYDFDRDEQRLPSGALPAAAAGGPGSIVRLDGTGW
                     RTLDVELRRGAVRGGGHYDGFRLRSRRFATPDWRGGAPGALTQAAEGRTRTAALWAEG
                     EAAIGDAVRLIVGARYEWWRAHGGRNFSLAPALDVAQPERTADGLSPKASLRWLPARG
                     WSVSLSAGQAYRFPTVSELYQAISTGPSLTVPDPNLRPERARSVELAGQWSDPAGRVR
                     LSLFREAISDALISQTAPLVPGSTALFSYVQNVEKVRTTGVELAFQRRGLIAAPLEVS
                     GSVTLVRPKVVEDPAFPAAEGKDIPQVPRRRATVVLTWHASEAASLTLAGRYSSRSFG
                     TIDNSDPVTHTFQGFGGFLVADARANLRIGKHWNAALGVENLGNDRYYLFHPFPQRTF
                     TGELTYRW"
BASE COUNT          710 a         1660 c         1569 g          625 t
ORIGIN      
        1 cgacccggct ccggcctccg acatggcgag cgatccgaca tgctcgcccg agatcagctt
       61 gggcaggtat ctcgccttct gctccgggct cgcccagcgg cggatctggt tgacgcacag
      121 gttcgagtgg gcgccgtagg agaggccgat cgaggccgag gcgcgggcca cctcctcctg
      181 ggcgacgaca tgctcgagat agccaaggcc caggccgcca tagtcctcct cgacggtgat
      241 gccgtggagt ccgagttcgc ccatctccgg ccacagctcc ttggggaacc agtcctcggc
      301 gtcgatccgg gcggcgagcg gcgcgatccg gtcggcggcg aagcggcggg tggtgtcgcg
      361 gatcgcgtcc gccatctcgc cgagcgcgaa gtcgagcccg atatcggcca tgcacatcct
      421 cctgtcggcg gcggccctag cagagccccg cggatttgaa caaggcgggg cgggcgccta
      481 tgtgcggccc atacgtcacc ccagcgaagg ctggggtcca taccgactca gcacgggcac
      541 tggcgccgca tggatgccag ccttcgctgg catgacgagg aaacataatg accgccaccg
      601 atcccatcgt catcctctcc tacgcccgaa cgccgatggg cagcttccag ggcgccctgg
      661 ccggggccag cgccaccgaa ttgggcgccg ccgccgtccg cgccgcggtc gagcgcgccg
      721 gagtcgccgg cgaggcggtc gacaagatct tcatgggctg cgtccttccc gccggcctcg
      781 gccaggcccc cgcccgccag gcggcgctcg gcgccggcct cccgcgctcg gtcgaggcga
      841 cgacgatcaa caaggtctgc ggctcaggca tgcaggcggc gatgttcgcc cacgacatga
      901 tcgcggccgg ctcggccgac ctcatcgtcg ccggcggcat ggagtcgatg tccaactcgc
      961 cctacctcct caagaagcac cgctccggcg cccgcatcgg ccacgacacg gtctacgacc
     1021 acatgatgct cgacgggctc gaggacgcct atgacaaggg ccgggccatg ggcaccttgg
     1081 ccgaggattc ggcgcgcgaa taccagttca cccgcggcga ccaggacgaa tatgcgatcg
     1141 agagcctgcg ccgggccaac gaggcgatcc gctccggcgc cttcgaccgc gagacgaccc
     1201 cggtcacggt gaagggccgc aagggcgacg agatcgtcga gcgcgacgag cagcccggca
     1261 agtccaatcc ggagaagatc cccggcctca agcccgcctt cgccaaggac ggcaccatca
     1321 ccgccgccaa tgccagctcg atcagcgacg gcgccgccgc cctcgtcatc gcgaggcagt
     1381 cggtcgccga ccgcctcgga gtcaggccgg tcgcgcggat cgtcgcccat gccgcgttcg
     1441 ccggcccgcc cgaccagttc accaccgccc ccgtccccgc cgtccgcaag gcgctggagc
     1501 gcgccggctg gagcgtcggc gacgtcgacc tctacgaggt caacgaggcc ttcgcctgcg
     1561 tcgcgatgat cgcgatgcgc gacctcggca tcccgcgcga cgccctcaac gtccacggcg
     1621 gcgcgaccgc gcttggccac ccgatcggag cgagcggcgc gcggatcctc accaccctca
     1681 tctcggcgct cgagacccac ggccagaagc gcggcctcgc caccttgtgc atcggcggcg
     1741 gcgaggcgac ggcgatggcg gtggagctgg cggattagga ggggcccgat aatgctcggc
     1801 aagcgcaagc tgcaggtctt cgtctccagc acctactccg atctgatcga ggagcggcag
     1861 gcggcggtca gcgccatcct caaggcgggc catattcccg cgggcatgga attgttcgcc
     1921 tccggcgacc ggtcgcagat ggacgtcatc aagcgctgga tcgacgaatc cgacgtctac
     1981 atggtgatct tgggcagccg ctacggctcc gtcgaggcga cgaccggact gagctacacg
     2041 gagctcgaat atgattatgc cgtcaacagc ggcaagccct tcttcgccct cgtcctcacc
     2101 gactcggcga tggacgagaa ggtgaagaag cacggcatcg ccgtcaccga gcaggaccat
     2161 cccgacaagc ttaaggcctt ccgctcgaag gtcatgagcc ggatgtgcgc tctgtgccag
     2221 gacaataagg acatccgcgg cggggtgtac gacagcatcc tggagatcct gcagacgcgc
     2281 gatcttcccg gctgggtgag gggcgattcc gtcgaggacc cgcgccccct ccacgaccag
     2341 atcgcgttgc tgaaatcgga gatccagcgc ctttcggccg agctcgacag cgcccgcgtc
     2401 gcgactcccg cttcggcggc ctcgaccggc agcgcgcgcg agttcgccac cctcgagaaa
     2461 gtgctcagga gcaagaagct ggaagccccg gccgaggtcg ccgggtcgga cgaggacgtc
     2521 agcacctcgg cctattcgct gctgatgacg aaccgcaacc tgctcgtccg gggctgggtg
     2581 atggcggtca acccggaggg gaagtccctg tggttcagga acaacctcat gccgatcctg
     2641 caggtgcacc ggctgataac ccgcgaggac atgggcccca agtaccaccg ctacaccatg
     2701 accgagctcg gcctggacct cctcgcctgg ttcgaccatc gggccgcgac gccggcgaaa
     2761 cccgccgaca aaagcaagcc ttccaagtga ccgcgctgcg ccggcgccgc ccgccgggcc
     2821 cgctcccccg cccctgctca ccaccgatag gtcagctccc ctgtgaaggt ccgctgcggg
     2881 aaggggtgga acagataata tctgtcgttg ccgagattct cgacgcccag cgccgcgttc
     2941 caatgcttgc cgatgcgcag gttcgcccgg gcgtcggcga cgaggaagcc gccgaagccc
     3001 tggaaggtgt gggtcaccgg gtcgctgttg tcgatcgtcc cgaacgagcg gcttgaatag
     3061 cggccggcga gggtcaggct ggccgcctcc gacgcatgcc aggtcagcac cacggtcgcg
     3121 cgccgccgcg gcacctgggg gatgtccttg ccctcggccg cggggaaggc gggatcctcg
     3181 accaccttgg ggcggaccag ggtgacgctg ccggagacct cgagcggcgc ggcgatcagc
     3241 ccgcgccgct ggaacgccag ctcgacgccc gtggtgcgca ccttctcgac gttctggacg
     3301 tagctgaaca gcgccgtcga cccgggcacc agcggcgccg tctgcgagat cagggcgtcg
     3361 ctgatcgcct cccggaacag cgacagccgc acccgcccgg ccgggtccga ccactggccg
     3421 gcgagctcga ccgagcgcgc tcgctcgggc cggaggttgg gatccggcac ggtcaggctc
     3481 gggccggtcg agatcgcctg gtagagctcc gagacggtcg ggaagcgata ggcctggccg
     3541 gccgacaggc tgacgctcca cccccgcgcc ggaagccatc tcaggctcgc cttgggcgag
     3601 aggccgtcgg cggtccgctc cggctgggcg acgtcgagcg ccggggcgag cgagaagttg
     3661 cgcccgccat gggcgcgcca ccattcgtag cgcgcgccga cgatgagccg cacggcatcg
     3721 ccgatcgcgg cctcgccctc ggcccacagc gccgcggtcc gggtccggcc ctcggccgcc
     3781 tgggtcaggg cgccgggcgc gccgccgcgc cagtcggggg tggcgaaccg gcggctgcgc
     3841 agccggaagc cgtcgtaatg gccgccgccg cggacggcgc cgcgccgaag ctcgacgtcg
     3901 agggtgcgcc agccggtccc gtccagccgg acgatcgagc ccggcccgcc ggccgccgcc
     3961 gccggcagcg cgccggaggg gagacgctgc tcgtcgcggt cgaaatcgta gaggcttccg
     4021 accgcccgcc agcgcaggcc cgccgcctcg ccctcggcgg cgaggccgtg catccagtgg
     4081 cgctggtcga gcgtgtagac gttgttggag aaggcgctcg ccggcacgct caccgtccgc
     4141 ccgtcgatgt tgagcgtgcc ggcataggcg ttgccgctga gataggtctc ggccgacgcc
     4201 tcggtgtcgt tgaggaacag gccgccgcga tagctgagcc gaagccccgg ccccggctcc
     4261 caggccagct tcagcttcag attgtcctgc tcatggtcct cgagcccggc ggcgccgagc
     4321 acgaagatcg gcgcccccgt ccggttgacg tcggcatagg cgccggtgac ccgggtgccg
     4381 gcgctccccg ccgccgccgg ccgggcgacg gtcgcatagg ccagcgggtg gctccggctg
     4441 gagacgtggc tggcgccggc gaaccaggcg aggtcgccgg tccgcccgcc cgccgtcccg
     4501 cccagctgga aggccggata gacgccctcg gtcccatatt gctcgaagct ctggacgctg
     4561 gtcg
//
LOCUS       DASVQZ010000363         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_367566, whole genome shotgun sequence.
ACCESSION   DASVQZ010000363 DASVQZ010000000
VERSION     DASVQZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367566"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VF547_10295"
     CDS             <1..1271
                     /locus_tag="VF547_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX8263249.1"
                     /translation="SVGLEALSDEDWTRHFGRFAPLPDNLPQPLALRYHGHQFRSYNP
                     DIGDGRGFLFAQLRDGAGRLLDLGTKGSGQTPYSRFGDGRLTLKGGVREVLATEMLEA
                     LGVETSKSFSLIETGEALERNDEPSPTRSAVLVRLQHSHIRIGTFQRLAYFRDSANLA
                     KLTGYCLRHYYGEDGGVDAPARLLAHVARAAARLAASYIAAGFVHGVLNSDNIAITAQ
                     SFDYGPWRFTPFWDGHFTAAYFDHAGLYAFGRQPEAIHWDVLQLAASLRAIAEADVLT
                     PSLDAFPADFQSRLVEAIFARLGIRPAGDERDSALLVALETALAAGTVGIDRFFFDWR
                     GGRRRGPSPAEEAYAGEPFAAFRDAVGGFEAVAGAADHPYWSGEAPCSMHIDEVESIW
                     SRIDEADDWTLFHDKIEAVRRMGEAMGAAA"
     gene            1304..2737
                     /gene="astD"
                     /locus_tag="VF547_10300"
     CDS             1304..2737
                     /gene="astD"
                     /locus_tag="VF547_10300"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEX8263250.1"
                     /translation="MATLSSIEPATGDTLWTGEVGDVDAEVARARSAWSAWAAKPLAV
                     RIETLRRFANTVRGQKDALADLIARETGKPLWETATEVDAVVGKVDISVSAYSERTSQ
                     RRLEGALGARVAVRHKPHGVLAVLGPYNFPAHLPNGHIVPALIAGNAVVFKPSEKTPA
                     VGEMLVRLYHEAGVPEDLVRCVQGGVEVGRALAASPGIDGLLFTGSARAGLALHKQFS
                     DTPQKILALEMGGNNPLVVWDAKDLGAAAVIAVQSAFMSSGQRCTAARRLIVQDGQHE
                     PLLAEIVKLAERIIVGPPHAQPAPFMGPVIDNQAADQLQEAFLDLMIKGGRPLKRLDR
                     PAEDRPFLTPAIIDVTDVADRPDAELFGPVLQVIRVPDFDSALAEANATRYGLAASLV
                     GGSPELYDRFWANIRAGVINWNKPTNGAPSNAPFGGLGLSGNHRPSAFYAADYCAYPV
                     TSSEAEGARASIGVGLRDPFAAKELEG"
BASE COUNT          406 a         1083 c          890 g          418 t
ORIGIN      
        1 cttccgttgg cctggaggcc ctcagcgacg aggactggac ccgccacttc ggccgcttcg
       61 cgcctctgcc cgacaacctc ccccagcccc tcgccctgcg ttatcacggc caccagttcc
      121 gctcctacaa cccggacatc ggtgacggcc gcggcttcct cttcgcccag ctccgcgacg
      181 gtgccgggcg cctgctcgac ctcggcacca agggctccgg ccagaccccc tacagccgct
      241 tcggcgacgg ccggctgacg ctcaagggcg gcgttcgcga ggtgctcgcc accgagatgc
      301 tcgaggcgct cggggtcgag acctcgaaga gcttctcgct gatcgagacc ggcgaggcgc
      361 tcgagcgcaa cgacgagccc tcccccaccc gctccgccgt gctcgtccgg ctccagcaca
      421 gccatatccg catcggcacc ttccagcgcc tcgcctattt ccgggattcg gccaacctcg
      481 cgaagctgac cggctactgc ctgcgccatt attacggcga agacggggga gtcgacgccc
      541 ccgcccggct gctcgcacat gtcgcccgcg cggcggcccg gctcgccgcc tcctacatcg
      601 ccgccggctt cgtccacggc gtgctcaaca gcgacaatat cgcgatcacc gcccagagct
      661 tcgactatgg tccgtggcgc ttcacgccct tctgggacgg ccatttcacc gccgcctatt
      721 tcgaccatgc cggcctctac gcgttcggcc gccagcccga ggcgatccac tgggacgtgc
      781 tccagctcgc cgcctcgctt cgcgccatcg ccgaggcgga cgttctgacg ccctcgctcg
      841 acgcctttcc ggcggacttc cagagccggc tcgtcgaagc gatcttcgcc cggctcggca
      901 tccgccccgc cggcgacgag cgcgattccg cgctgctcgt cgccctcgag accgccctcg
      961 ccgccgggac cgtcgggatc gaccgtttct tcttcgactg gcgcggcgga cgaaggcgcg
     1021 gcccctcccc cgccgaggag gcctatgcgg gcgagccgtt cgccgccttc cgcgacgcgg
     1081 tcggcggctt cgaggccgtc gccggggcgg ccgaccatcc ctattggtcc ggcgaggcgc
     1141 cctgctcgat gcatatcgac gaggtcgaat cgatctggag ccgcatcgac gaagccgacg
     1201 actggaccct tttccacgac aagatcgagg ccgtcaggcg catgggcgag gcgatgggcg
     1261 ccgcggcttg accgccgcag ccaaaaatgg tgggtcgtcc gccatggcga cattgagctc
     1321 gatcgaaccg gccacggggg acacgctctg gaccggcgag gtcggcgacg tcgacgcgga
     1381 ggtcgcccgc gcccgctccg cctggtcggc ctgggcggcc aagcctctcg ccgtcaggat
     1441 cgagaccctg aggcgcttcg ccaacaccgt gcgcggccag aaggacgcgc tcgccgacct
     1501 catcgcccgc gagaccggca agccgctctg ggagaccgcc accgaggtcg acgcggtggt
     1561 gggcaaggtc gacatctcgg tctccgctta ttccgagcgc acctcgcagc gccgcctcga
     1621 aggcgcgctc ggcgcccgcg tcgcggtccg ccacaagccg cacggcgttc tggccgtgct
     1681 tgggccctat aatttcccgg cgcacctgcc caacggccac atcgtgccgg cgctgatcgc
     1741 cggcaatgcg gtcgtgttca agccgtcgga gaagaccccg gcggtcggcg agatgctggt
     1801 ccggctctac catgaggccg gggttccgga agatctcgtc cgctgcgttc agggcggtgt
     1861 cgaggtcggc agggcgctcg ccgccagccc cggcatcgac ggcctgctgt tcaccggctc
     1921 ggcccgcgcc ggcctcgccc tccacaagca attctcggat acgccgcaga agatcctcgc
     1981 cctcgagatg ggcggcaaca acccgctcgt cgtctgggac gccaaggacc tcggcgccgc
     2041 cgcggtgatc gccgtccagt ccgccttcat gagctcgggc cagcgctgca cggcggcccg
     2101 gcggctgatc gtccaggacg gccagcacga gccgctgctg gccgagatcg tcaagctggc
     2161 cgagcggatc atcgtcggcc cgccccatgc ccagccggcg ccgttcatgg gcccggtgat
     2221 cgacaaccag gccgccgacc agctccagga agccttcctc gatctgatga tcaagggcgg
     2281 ccggccgctg aagcggctcg accgtcccgc ggaggaccgg cccttcctga ccccggcgat
     2341 catcgacgtc accgacgtcg ccgaccgccc cgacgccgag ctgttcggcc cggtccttca
     2401 ggtcattagg gtccccgatt tcgactccgc cttggccgag gcgaacgcca cccgctacgg
     2461 cctcgccgcc tccctggtcg gcggcagccc ggagctctac gaccgcttct gggccaacat
     2521 ccgcgccggc gtcatcaact ggaacaagcc caccaacggc gccccctcca acgccccctt
     2581 cggcggcctc ggcctcagcg gcaaccaccg ccccagcgcc ttctacgccg ccgattactg
     2641 cgcctatccg gtgaccagct ccgaggcgga aggcgcaagg gccagcatcg gcgtaggcct
     2701 cagggacccg ttcgcggcga aggagctgga gggataaata tcctccccat cgaagatggg
     2761 gagggggacc acccggaggg tggtggaggg gtaatcg
//
LOCUS       DASVQZ010000364         5854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_557323, whole genome shotgun sequence.
ACCESSION   DASVQZ010000364 DASVQZ010000000
VERSION     DASVQZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557323"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VF547_10305"
     CDS             complement(<1..964)
                     /locus_tag="VF547_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8263251.1"
                     /translation="MAAPMIPPPGAPDFLARHGWAGAEIRPLAGDASFRRYFRVHRGD
                     ETAVLMDAPPEHEDVGPFLAVAGHLLDRGFHPPRPLGIDRDKGLLLLEDFGDDRVGPL
                     LQREPQREREVYDAAIDILARLAGQPAPPDVPLYDDSVMTREVLLFSEWYLPALGIEV
                     DEQAYLSAWRDAWGDVLRIVEANPVLVLRDYHADNLMVLPGRSELGLLDFQDALAGHP
                     AYDLVSLLQDARRDVAPELEEAMIERYCQRANVADRDLFRAHYEVLGAQRNTKILGIF
                     TRLWKRDGKPFYPTMHPRIWNYLERNLAHPALAPVRAWFDSNVPA"
     gene            complement(949..1386)
                     /gene="tsaE"
                     /locus_tag="VF547_10310"
     CDS             complement(949..1386)
                     /gene="tsaE"
                     /locus_tag="VF547_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX8263252.1"
                     /translation="MRLEGPEATEAFGRRLARALRAGDVVALFGELGTGKTTMARGLL
                     AGLGHSGEVASPTFPIVIPYEALVLPAWHVDLYRVEDPAEIEQLALDEALQDGALIVE
                     WPERMGGALWPHSLRLTLRRDGEGVRALTAGVPAAWEARWPPR"
     gene            complement(1386..3731)
                     /locus_tag="VF547_10315"
     CDS             complement(1386..3731)
                     /locus_tag="VF547_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8263253.1"
                     /translation="MMTLSPTALAAIALFCGAWLALALWATARGLGRARAAAGRIDDT
                     RHHEALLAASPALPLIVHRDGRLQEAERTALALGLAAAPERLADLGEAFSEEDRERLA
                     TQVEASAAAAGNFTLSLRPAGSSRVYRVQGGPAPPSYPPGTVLLWLTDATEGEEEMAE
                     LRQRSGRLAAALDALSGLIEAAPFPIWHRGPDLRLAMVNGAYVAAVEAEDARAVVEGG
                     IELVDDGEEAARAEAAAVREARQAHSRTVPATIGGERRMMRVVEVPLGEASVAGYAID
                     VEDREQARSELARFVHAQRDMLDRLSAGVAQFGRDRSLIFFNQPFARLFALRPDFLAD
                     PPEFNRLLDAMRETGHLPEVRDYPEWKKEHDRWFTGGLAAEEEDWLLPGGRHLRVVAQ
                     PLPDGGLLLIFEDRTEQLRLASARDTLLRVRTATFDNLFEAVGVFASDGRLHLWNNRF
                     RELWEFEEEQLAGSPRIDSLTPHIGRKLRNASHAALVRELVRSATVERKQRTGRVAFT
                     DGREFEFAAVPLPDGNALFTMLDVTASRTVEAALRERTLALEQADRLKTAFVANMSYE
                     LRTPLTSIGGFAEMLAGGYAGELAPTARDYVAAILESVARLGALIDDVLDLTQSDSGS
                     LLLAEDKVDLRKLCAEAAEAFREPAARKAIDFAVEIESGAGIVAGDRARLRQSLDNVL
                     KNAFAYTDEGGRVLLHATGDRKAAAITVSDNGRGIAPADQARVFDRFSRTAEPRGADE
                     AALGLGLPLARQFVEAHGGTIELLSEPGEGTTVTLRLPRKR"
     gene            complement(3918..>5854)
                     /locus_tag="VF547_10320"
     CDS             complement(3918..>5854)
                     /locus_tag="VF547_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8263254.1"
                     /translation="DQGLGRVRTVAAAGGAARDVTSQPGHYARPRFSPDGRTIVFEQG
                     QGGFLTSPKWSENPGIYRVAATGGTPVRVSREGTEPQFGASNERIFMIVPANNKRQLV
                     STDLNGEARHVHSTGEMVSDYQVSPDGQYVAFRQNYQAFVMPLMPGTQDVAADAKGAP
                     LPVTKASAVGADFIHWSAGGRRLHWSTGPTVFTADTAAMFGNSPPAENAPKFQPPTTG
                     VSLAMEVAADRPSGIVALTGARIVTMAGADGGIVDDGVILVRGDRIAAVGRRGEVAIP
                     AGAKTVDVAGKTIIPGLVDAHAHGPQGQDGLVPQQNWSSIVNLAMGSTTIHDPSASAA
                     EIFVASEMQRAGKILAPRIFSTGEIIYGAKAADVYAEINTFEDALAHVRRLKAQGAYS
                     VKNYNQPRREQRQMVVAAAQREGMEVVPEGGSLYNMDISLIQDGNATLEHNLPVQVFY
                     DDVVSLWTQSKTNYTPTLVVAYGGLAGDPYWRQHTDVWRHPLLSRHAPPTLLAAQNVR
                     RTMAPEEDFVDDDTAREAKKLADRGVQVSIGAHGQQAGLGPHWEMWSFVRGGWTPLEA
                     LRAGTIMPAVSLGYARDVGSIEAGKLADLVVLDADPTVDIRNSERIHRVMLGGRLYDP
                     VTMNEVVTGTRVRAPYWWED"
BASE COUNT          842 a         2044 c         2112 g          856 t
ORIGIN      
        1 ccgccggcac gttggagtcg aaccacgccc gtaccggcgc cagcgccgga tgggcgagat
       61 tgcgctccag atagttccag atgcgcgggt gcatggtcgg gtagaagggc ttgccgtcgc
      121 gcttccagag ccgggtgaag atgccgagga tcttggtgtt ccgctgcgct ccgaggacct
      181 cgtaatgggc ccggaagagg tcgcggtcgg cgacgttggc ccgctggcaa taacgctcga
      241 tcatcgcttc ctcgagctcg ggagcgacgt cgcggcgggc gtcctggagg agggagacca
      301 gatcgtaggc gggatggccg gcgagcgcgt cctggaaatc gagcaggccg agctcggacc
      361 ggcccgggag caccatcaga ttgtcggcat gatagtcgcg caggaccagg accggattcg
      421 cctcgacgat tcgaaggacg tctccccatg cgtcgcgcca tgccgaaaga taggcttgct
      481 cgtcgacctc gatgcccaat gccggcaggt accattcgga gaagaggagc acttcgcggg
      541 tcatcacgga atcgtcatag agcgggacgt cgggcggagc cggctggccc gcaagccgcg
      601 ccagtatgtc gatcgcggcg tcgtagactt cgcgctcgcg ctgcggctcg cgctgcagca
      661 gcggaccgac ccggtcgtcc ccgaaatcct cgagcaggag gaggcccttg tcgcggtcga
      721 tgccgagcgg ccgcggagga tggaagccgc ggtccagcag gtggcccgcg acggccagga
      781 acggcccgac atcctcatgc tccggcggag cgtccatcag taccgcggtt tcgtcgccgc
      841 ggtggacccg gaaatagcgg cggaagctcg cgtcgccggc cagcggcctt atctccgcgc
      901 cggcccagcc gtgcctcgca aggaaatccg gcgcccctgg gggcggaatc atcggggcgg
      961 ccatcgcgcc tcccaagcgg cgggcacccc ggctgtcaag gcgcggacgc cctccccgtc
     1021 ccggcgcagg gtgaggcgaa gcgaatgcgg ccacaaggcg ccgcccatcc gctccggcca
     1081 ttcgacgatc agggcgccgt cctggagcgc ctcgtccagc gccagctgct cgatctcggc
     1141 cgggtcctcg acccgataga ggtcgacatg ccaggccggg aggacgagcg cctcataggg
     1201 gatgacgatc ggaaaggtgg ggcttgcgac ctcgcccgag tggccgaggc cggccagcag
     1261 gcctcgcgcc atcgtcgtct tgccggtgcc gagctcgccg aacaaagcga cgacgtcgcc
     1321 cgcccgaagc gcccgcgcca gccgccggcc gaacgcctcg gtcgcctccg gcccctccag
     1381 ccgcatcagc gcttccgcgg caggcgcagg gtgacggtgg tgccctcgcc cggctccgac
     1441 agaagctcga tcgtcccgcc atgggcctcg acgaactggc gcgcgagcgg gaggccgagg
     1501 ccgagcgcgg cctcgtcggc gccgcgcggc tcggcggttc ggctgaagcg gtcgaacacc
     1561 cgggcctggt cggcgggggc gatcccccgg ccgttgtcgg agaccgtgat cgccgccgcc
     1621 ttgcggtcgc cggtcgcgtg gagcagcacc cggccgccct cgtcggtata ggcgaaggcg
     1681 ttcttgagca cgttgtcgag cgactgccga agccgggccc ggtcgcccgc gacgatgccg
     1741 gcgccgctct cgatctcgac cgcgaagtcg atcgccttcc tcgccgccgg ctcacggaac
     1801 gcctcggccg cctccgcgca cagcttcctc agatccacct tgtcctcggc gagcagcagg
     1861 ctgccgctgt cgctctgggt gaggtcgagc acgtcgtcga ttagggcgcc gagccgggcg
     1921 accgattcga ggatcgcggc gacatagtcc cgagcggtcg gcgccagctc gccggcatag
     1981 cctccggcca gcatctcggc gaagccgccg atcgaggtca ggggggtgcg aagctcgtag
     2041 ctcatgttgg cgacgaaggc cgtcttcagc cggtccgcct gctccagcgc cagggtgcgc
     2101 tcgcgcagcg ccgcctcgac ggtgcggctg gcagtgacgt cgagcatcgt gaacagggcg
     2161 ttgccgtcgg gcagcggcac cgccgcgaac tcgaactcgc ggccgtcggt gaacgccacg
     2221 cgccccgtcc gctgcttgcg ctccacggtc gcgcttcgca ccagctcgcg gaccagggcg
     2281 gcgtggctcg cattcctgag cttgcggccg atatgggggg tcagcgaatc gatccgcggg
     2341 ctcccggcga gctgctcctc ctcgaactcc cagagctcgc ggaagcggtt gttccacaga
     2401 tgcaggcgcc cgtccgaggc gaagaccccg accgcctcga acagattgtc gaacgtggcg
     2461 gtgcgcaccc tgagcagggt gtcgcgggcg gaggcgaggc gaagctgctc ggtccggtcc
     2521 tcgaagatca gcagcagccc gccgtccggg agcggctgcg cgacgacgcg caggtggcgc
     2581 ccgccgggaa gcagccagtc ctcctcctcg gcggcgaggc cgccggtgaa ccagcggtca
     2641 tgctccttct tccattccgg atagtcgcgg acctcgggca ggtggccggt ctcgcgcatc
     2701 gcgtcgagca gccggttgaa ctcagggggg tcggcgagga aatcgggccg cagcgcgaac
     2761 aggcgcgcga agggctggtt gaagaagatc aggctgcggt cgcggccgaa ctgggcgact
     2821 ccggccgaga gccggtcgag catgtcgcgc tgggcatgga cgaagcgcgc gagctccgag
     2881 cgcgcctgct cgcggtcctc gacgtcgatc gcgtagccgg cgacgctcgc ctcgccgagc
     2941 ggcacctcga ccacccgcat catccgccgc tcgcctccga tcgtcgcggg aacggtgcgc
     3001 gaatgcgcct gccgggcctc gcgcacggcc gccgcctcgg cgcgggccgc ctcctcgccg
     3061 tcgtcgacca gctcgatgcc gccctcgacc acggcccggg cgtcctcggc ctcgaccgcg
     3121 gccacatagg ccccgttgac catggcgagg cgcaggtccg gtccgcgatg ccagatcggg
     3181 aagggcgccg cctcgatcag gccggacaag gcgtcgagcg ccgccgcgag ccggccgctg
     3241 cgctggcgca gctctgccat ctcctcctcg ccctcggtcg cgtcggtcag ccacagaagc
     3301 acggtgcccg gcggatagga ggggggcgcg gggcctccct ggacgcggta gacgcgcgac
     3361 gagccggcgg ggcgcagcga cagcgtgaag ttccccgcgg cagcggcgga cgcctccacc
     3421 tgcgtcgcga ggcgctccct gtcctcttcg ctgaaggctt cgccgaggtc ggctaggcgc
     3481 tcgggggccg ccgccagccc gagcgcgagc gcggtccgct ccgcttcctg gagacgtccg
     3541 tcgcggtgga cgatcagggg gagcgccggg gacgcagcca gcagggcctc gtggtgccgg
     3601 gtgtcgtcga tcctgccggc cgcggcgcgg gcgcggccca ggccgcgcgc agtcgcccag
     3661 agggcgaggg cgagccaggc gccgcagaag agcgcgatcg ccgccagcgc ggtcggggag
     3721 agggtcatca tggaggccct tctctaacgg ggcgcatccc tgtccggaag ggcgagcggc
     3781 catcctaatt cctccccgag atttctcggg gagggaccgc gcgaagcgcc gtggaggggg
     3841 tcttcggaat cggtaaggcc gctccaccac ccttcgggtg gtccccctcc cctgctaatg
     3901 caggggagga atcgagatca atcctcccac cagtaaggcg ctcggacccg ggtgccggtg
     3961 accacctcgt tcatcgtcac cggatcgtat agccggccgc cgagcatgac ccggtggatc
     4021 cgctcgctgt tgcggatgtc gaccgtggga tcggcgtcga gaacgaccag gtcggcgagc
     4081 ttgccggcct cgatcgagcc cacgtcgcgg gcatagccga gcgagaccgc cggcatgatc
     4141 gtgccggccc ggagcgcctc cagcggggtc cagccgccgc gcacgaacga ccacatctcc
     4201 caatgcggcc cgagcccggc ctgctgcccg tgggcgccga tcgacacctg cacgccccgg
     4261 tcggcgagct tcttcgcctc gcgcgcggtg tcgtcgtcga caaagtcctc ctcgggcgcc
     4321 atcgtccgcc gcacgttctg ggcggcgagc agcgtcggcg gcgcgtgcct cgacaggagc
     4381 ggatgccgcc agacgtcggt gtgctggcgc caataaggat cgccggcgag cccgccatag
     4441 gcgactacca gggtcggcgt gtagttggtc ttgctctggg tccacaggct gacgacgtcg
     4501 tcgtagaaga cctggaccgg caggttgtgc tctagcgtcg cgttgccgtc ctggatcagc
     4561 gagatgtcca tgttgtagag cgacccgccc tccgggacga cctccatccc ctcgcgctgc
     4621 gccgccgcga ccaccatctg gcgctgctcg cgccggggct ggttgtagtt cttgacgctg
     4681 taggcgccct gggccttcag gcggcggacg tgggccagcg catcctcgaa cgtgttgatc
     4741 tcagcataga cgtcggccgc cttggcgcca tagatgatct cgcccgtcga gaagatgcgc
     4801 ggagcgagga tcttgccggc gcgctgcatt tcggaagcga cgaagatctc cgccgcgctc
     4861 gcggacggat cgtggatcgt cgtcgagccc atggcgaggt tgacgatcga agaccagttc
     4921 tgctgcggga cgaggccgtc ctggccctga ggcccatggg catgggcgtc gaccaggccg
     4981 gggatgatcg tcttgccggc gacgtcgacc gtcttggctc cggccggaat cgcgacctcg
     5041 ccgcggcgtc cgacggcggc gatcctgtcg cctcggacca ggatcacccc atcgtcgacg
     5101 atgccgccgt cggcccccgc catggtcacg atcctcgccc cggtgagagc gacgattccg
     5161 ctcggccggt cggcggcgac ctccatcgcc agcgacaccc cggtggtcgg cggctggaac
     5221 ttgggagcgt tctcggccgg cggcgaattg ccgaacatgg ccgcggtatc ggcggtgaag
     5281 actgtcggcc cggtgctcca atggagcctc cggccccccg ccgaccagtg gatgaagtcg
     5341 gcgccgaccg cgctcgcctt cgtcaccgga agcggggcgc ccttggcgtc ggcggcgacg
     5401 tcctgggtgc cgggcatgag cggcatcacg aaggcctgat aattctggcg gaaggcgaca
     5461 tactggccgt ccggggagac ctgatagtcg ctgaccatct cgccggtcga atggacgtgg
     5521 cgggcctcgc cgttgaggtc ggtgctgacc agctggcgct tgttgttggc cgggacgatc
     5581 atgaagatac gctcgttgga agcgccgaac tgcggttcgg tgccttcgcg gctgacgcga
     5641 acgggcgtgc cgccggtcgc cgcgacccgg tagatgccgg gattctccga ccatttgggc
     5701 gaggtgagga aaccgccctg cccctgctcg aagacgatcg tcctgccgtc gggcgagaag
     5761 cgtgggcggg cataatggcc cggctgcgag gtcacgtcgc gggcggcgcc gccggccgcc
     5821 gcgacggtcc tgacccgtcc gaggccctgg tcgg
//
LOCUS       DASVQZ010000365         8388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_76520, whole genome shotgun sequence.
ACCESSION   DASVQZ010000365 DASVQZ010000000
VERSION     DASVQZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76520"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VF547_10325"
     CDS             <1..1283
                     /locus_tag="VF547_10325"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX8263255.1"
                     /translation="LERLADLLERERDALMRLCVLEARKTIPDALAEVREAVDFCRYY
                     AAQARKGLQPVELPGPTGERNVLRMGGRGAWICIAPWNFPLAIFLGQVSAALVAGNAV
                     VAKPAPQTPEIAAYAVGLAHGAGIPEDALILAPGGPDMGAALVEDGRIAGIAFTGSTA
                     TAKRIARELLNDDERPIIPFIAETGGINAMIVDSTALPEQVVVDVVTSAFRSAGQRCS
                     ALRVLLLQEEIAGRTLEMLAGAMDSLIVGDPSRPDTDVGPVIDEGAYDRLMAHREETK
                     ARWIKTVAAPEQGLYVPPTLIGVDRVEDVRKEWFGPLLHVATWKAGQLAETVERVNRS
                     GFGLTMGLHSRIARAAETVEALATVGNLYVNRSMIGAIVGSQPFGGEGLSGTGPKAGG
                     PHYLPRFCAERVTSTDTTSAGGNATLLSLEDVGL"
     gene            1283..1564
                     /locus_tag="VF547_10330"
     CDS             1283..1564
                     /locus_tag="VF547_10330"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX8263256.1"
                     /translation="MALHVAEFESSAIDRAAYEDEALTLDIWYAGGDRYRYSEVPMAV
                     YRRLESAPSAGEFANREIKPHYRCEIEERRPLPPRSGRELTGGILHRSG"
     gene            complement(1665..2444)
                     /locus_tag="VF547_10335"
     CDS             complement(1665..2444)
                     /locus_tag="VF547_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8263257.1"
                     /translation="MSLLGLCPVAAQAGELRDFCPDRPGLGTPPCTIDAGHFDVELGL
                     ADWTLDRDAGTRTDTIEAGQLLVRIGLTDSLEGQIGWTAIGHVRERDRAAGTVASASG
                     VGDLSIALRQNLVSPDGSGFSLALMPYATLPTGNEVLGAGEWTAGLLIPASYDLGGGI
                     QLGLTAQAEAAADEDRRGRHLAYGGVVGLALPLSDAVGATFEIAATRDEDPSGRSIEW
                     LAGLSAGWMATDALQLDAGANLGLNAAAADVQLYVGISRRF"
     gene            2543..3556
                     /locus_tag="VF547_10340"
     CDS             2543..3556
                     /locus_tag="VF547_10340"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX8263258.1"
                     /translation="MTDLDAFIAGLPKAELHMHIEGSLEPELMFELARRNAVEIPFGS
                     VEEVRAAYRFSNLQDFLDIYYQGAAVLLTEEDFKDLALAYFDRAAADSVAHAEIFFDP
                     QTHTARGVPFDVVMKGLLAGMDEALERHGLSSKLILCFLRHLDEEDAFATLREAEPWL
                     DRIEAVGLDSSELGHPPEKFARVFAAAAERGLKRVAHAGEEGPPDYVLQALDLLNIDR
                     LDHGNRSLENPGLVARLVRDGMTLTVCPLSNLKLCVVGDMTAHPIDRMLREGLKVTIN
                     SDDPAYFGGYVNDNYRAAAAGRNLTRDDLATLARNSFEGSFLDEPAKEAQLARLGAYL
                     ARV"
     gene            complement(3672..4817)
                     /locus_tag="VF547_10345"
     CDS             complement(3672..4817)
                     /locus_tag="VF547_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8263259.1"
                     /translation="MADGATGNFLSKSSALAAEAQAWVGGNWAGILVAAAIGIGIALA
                     MLAVRSLGCRLIARLGTDPHWPAIFARALAKTKLYFIVFTSAELVAEHANTPPALLNA
                     IHVGFVIAAALQAAIWARELILGYVAHRTGGEQDQSNLASAIGIIRLLVTVTLFAIAV
                     ILILDNLGVNVTGLVAGLGIGGIAIGLAAQGIFSDLFAALSILFDKPFRRGDTISFDT
                     HTGTVERIGLKSTRIRSVGGEELIVSNANLLNTRLQNWTRLERRRALVALSLVYQTPP
                     ELLETVPAELEAIVRRRKHARFDRAHLVKFAPSSLDYELVFFVDSHQLADFLEARQAV
                     MLDILRRFAELGIQFAYPSQASFTAAPDGRLIMPYPEAEGGAAERLT"
     gene            complement(4817..5827)
                     /locus_tag="VF547_10350"
     CDS             complement(4817..5827)
                     /locus_tag="VF547_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX8263260.1"
                     /translation="MLCFVDDRTLPGITRRRRGRYWQYFAPDGSRITDRDEIDRLNAV
                     GMPPAYERCWFCPKPNGHVQATGYDARGRKQYRYHAEFRARQEAAKYERLAAFGQALP
                     KLRKRVEEDLQGKPTARDTVLAAVVRLIDETRMRVGNEEYAKDNKSFGATTLRNRHAK
                     VEPGRLKISYTGKHGIKRTVTLTDRNLVRIARKTQDLPGQNLFEYVNDDGDNCPVTST
                     DVNAYIREAMGADFTAKDFRTWGASTIAFEEMIRRYRKHGKVNLKSVIEPVAEALGNT
                     AAISRKSYVHPAVIDAARDAGAIGERKLPRASKYLSSAERGLIEFLEALDEKAEAAAK
                     AA"
     gene            5912..6460
                     /locus_tag="VF547_10355"
     CDS             5912..6460
                     /locus_tag="VF547_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX8263261.1"
                     /translation="MPNIADAKILMIATDGFEDSELIEPRRILRERGADVTVASLEKR
                     EIRGSHGTSVEADTAIGEVDADGYDALVLPGGTRNADKLRDDERVVAAVRAFAEAGKP
                     VAAICHGPWLLVEADVIRGRTVTAWSSIHTDLRNAGADVIDQEVVIDGNIVTSRKPDD
                     IPAFTEALIVLYEKADAPLETV"
     gene            complement(6424..7794)
                     /locus_tag="VF547_10360"
     CDS             complement(6424..7794)
                     /locus_tag="VF547_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HEX8263262.1"
                     /translation="MRDLAFILFLAALFGLGLRRPFLFVLAYIYIDVVSPQRLSYYLI
                     NSVPVSMIAACLALVGWLVAEKKAEMRFSVRQGLIAILLCYTFYTTRNADFPIEAAEK
                     WDWAWKVLAFAIFLPLTLRSKLRIEATILYLILSAAAIIIVGGIKTVLSGGGYGVLNL
                     MVNNNSNLYESSTISTVAIAIIPMILWLARFGTVFPPDWRVKTFAAALIFACLLMPIG
                     TEARTGLVCIGVLGVLMLRDVKRRILYLTGAALLCAASIPFLPASFTGRMSTIQGYES
                     DSSANVRLAVWAWTWDYVQEHPLGGGFGAYRQNKIQVRTVSTQVVGGVESVTTRTEMD
                     QGRAYHNSYFEMLGEQGFPGLILFLMIHGAGLVRLEILRRRYRKAEGDDAWIAPLATA
                     LQHFQIIYLIGSLFVGIAYQPYPWLVFAVAIGLDAYVARRARGAARKPFLAGPQTVSR
                     GASAFS"
     gene            complement(7787..7930)
                     /locus_tag="VF547_10365"
     CDS             complement(7787..7930)
                     /locus_tag="VF547_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263263.1"
                     /translation="MIDNFSLALTHVLLLIVAWRILMRPDLDDDSAPAAPAPPPGKGA
                     GDA"
     gene            8020..>8388
                     /locus_tag="VF547_10370"
     CDS             8020..>8388
                     /locus_tag="VF547_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263264.1"
                     /translation="MGALFLVRSADPDAADSAMARARVQFGRHGLAGLAERAFPGWRL
                     LHAPHILGGPESLLEAGEDFVAVAGTLTWDDMMGRPALEALLRTVSLPEPDWSRLGGQ
                     FAALFRRQGRAFLLTDHFAMF"
BASE COUNT         1344 a         2805 c         2941 g         1298 t
ORIGIN      
        1 gcctcgagcg gctcgccgac ctgctcgagc gcgagcgcga cgcgctgatg cggctgtgcg
       61 tgctggaagc gcgcaagacg atcccggacg ccctcgccga ggtgcgcgag gcggtcgatt
      121 tctgccgtta ctatgcggct caggcacgga aggggctgca gccggtcgag cttcccggcc
      181 cgaccgggga gcgcaacgtg ctgcgcatgg gcggccgcgg cgcctggata tgcatcgcgc
      241 cctggaactt cccgctcgcc atcttcctcg gccaggtcag cgccgccctc gtcgccggca
      301 acgccgtggt cgccaagccc gccccgcaga cgcccgagat cgccgcctat gcggtcggcc
      361 tcgcccatgg cgccgggatc ccggaggacg cgctgatcct ggcccccggg ggccccgaca
      421 tgggcgccgc cctggtcgag gacggccgga tcgccgggat cgccttcacc ggctccaccg
      481 ccaccgccaa gcgcatcgcc cgcgagcttc tcaacgacga cgagcggccg atcattccgt
      541 tcattgccga gaccggcggc atcaacgcga tgatcgtcga ttcgaccgcg cttcccgagc
      601 aggtcgtggt cgacgtcgtc acctccgcct tccgctccgc cggccagcgc tgctcggcgc
      661 ttcgcgtcct tctcctccag gaggagatcg ccgggcggac gctggagatg ctcgcggggg
      721 cgatggacag cctgatcgtc ggcgaccctt ccaggccgga caccgacgtc gggccggtca
      781 tcgatgaggg cgcctacgac cggctcatgg cgcaccgcga agagacgaag gcgcgctgga
      841 tcaagaccgt ggcagccccc gagcagggcc tctacgtgcc tccgaccctg atcggggtcg
      901 accgggtcga ggacgtccgc aaggaatggt tcgggccgct gctccacgtc gccacctgga
      961 aggcggggca gctcgccgag acggtcgagc gggtcaaccg ctcaggcttc ggcctcacca
     1021 tgggcctgca cagccggatc gcccgcgccg ccgagacggt cgaggcgctc gcgacggtgg
     1081 gcaatctcta cgtcaaccgc tcgatgatcg gcgccatcgt cggctcgcag ccgttcggcg
     1141 gcgagggcct gtccggcacc ggccccaagg cgggcggacc tcattatcta ccgcgcttct
     1201 gcgccgaacg ggtgaccagc accgacacca cctcggccgg gggcaatgcg accttgctgt
     1261 cgcttgagga cgtggggctc tgatggcgct ccacgtcgcc gagttcgaat ccagcgccat
     1321 cgatcgcgct gcctatgagg acgaggctct gactctcgac atctggtatg cgggcggcga
     1381 ccgctaccgc tattccgagg tcccgatggc ggtttatcgg aggctggaat cggcgccatc
     1441 ggccggcgaa ttcgcgaacc gcgagataaa gccgcattac cgctgcgaga tcgaggaacg
     1501 tcggccgctt ccgccccggt caggacgaga gttgaccgga ggcatcctcc accgttcggg
     1561 ctgagcttgt cgaagccctg ctcttgcctt ttgaaaaggg acgggaagcc cttcgacaag
     1621 ctcagggcga acgggcgtgg cgattcaact gaaccttcaa ggccctagaa gcgccgagag
     1681 atcccgacgt agagctggac gtcggcggcc gcagcgttga ggccgagatt ggcgccggcg
     1741 tcgagctgga gcgcgtccgt cgccatccag ccggcggaca gcccggccag ccattcgatc
     1801 gagcggccgg aagggtcctc gtcgcgggtc gcggcgatct cgaacgtcgc gccgaccgcg
     1861 tcggacagcg gcagcgcgag gccgacgacc cctccataag ccaggtggcg gccccggcgg
     1921 tcctcgtcgg ccgccgcttc cgcctgcgcc gtcaggccga gctggatacc gccgcccaga
     1981 tcgtagctcg ccgggatgag caggcccgcc gtccactcgc ccgcgccgag tacctcgttg
     2041 ccggtgggaa gagtggcgta gggcatcagc gccagcgaga agccggaacc gtccgggctg
     2101 accagattct gccggagggc gatcgacagg tcgccgactc ccgaggcgct ggccacggta
     2161 ccggctgcgc ggtcgcgctc gcggacatga ccgattgcgg tccagccgat ttgcccctcg
     2221 aggctgtcgg tgagaccgat tcggacgagg agctggcccg cttcgatcgt gtcggtgcgg
     2281 gtgccggcgt cccggtcgag cgtccagtcg gcgaggccga gctcgacgtc gaaatgcccc
     2341 gcgtcgatcg tgcagggcgg ggtgccgagc ccgggccggt cggggcagaa gtcccgcagc
     2401 tcgccggcct gagccgcgac ggggcagagg ccgaggaggc tcaatccaaa gaggatacgt
     2461 cccggcccca tgacggcaca cataggccgt cggccgagtc ttgccagtgg aaaagagcgg
     2521 cggctgccga taatcaaggc cgatgaccga tctcgacgcc ttcatcgcgg gcctgcccaa
     2581 ggccgagctt cacatgcata tcgagggcag cctcgagccc gagctgatgt tcgagctggc
     2641 tcgccgcaac gcggtggaaa tcccgttcgg aagcgtcgag gaggtgcggg cggcgtatcg
     2701 cttctcgaac ctgcaggact tcctcgacat ctactatcag ggcgccgccg tgcttctgac
     2761 cgaggaggac ttcaaggatc tcgccctcgc ctatttcgac cgcgcggcgg cggattcggt
     2821 ggcgcatgcg gaaatcttct tcgatcccca gacccacacc gcccgcggcg tcccgttcga
     2881 cgtggtgatg aaagggctgc tggccggaat ggacgaggcg ctcgagcggc acggcctcag
     2941 ctcgaagctg atcctgtgct tcctgcgcca tctcgacgag gaggacgcct tcgccaccct
     3001 tcgcgaggcg gagccgtggc tcgaccggat cgaggcggtg ggcctcgatt cctccgagct
     3061 cggccatccg cccgagaagt tcgcccgagt gttcgccgcc gccgccgagc gcgggctgaa
     3121 gcgggtcgcc catgccggcg aggaaggccc gcccgattac gtcctccagg cgctcgatct
     3181 tctgaacatc gacaggctcg accacggcaa ccgctcgctc gagaatcccg gcctcgtcgc
     3241 ccggctggtg cgcgacggga tgaccctgac cgtgtgcccg ctctccaacc tgaagctgtg
     3301 cgtcgtcggc gacatgacgg cgcacccgat cgaccggatg cttcgcgagg ggctgaaggt
     3361 gacgatcaac tcggacgatc ccgcctattt cggcggctat gtgaacgaca attaccgggc
     3421 cgccgccgcg gggcggaacc tgacccgcga cgacctggcg acgctggcgc gcaacagctt
     3481 cgaggggtcg tttctcgacg agccggcgaa ggaagcgcag ctggcgaggc tgggcgccta
     3541 tctggcccga gtctgaacgc ccctcccttt tccgttcgcc ctgagcctgt cgaagggctt
     3601 tccttccttc cctgcagtgc ggcgaggaag gagagggctt cgacaggctc agcccgaacg
     3661 gtgggatggt ttcaggtcag ccgctccgcc gcgccgccct ccgcttccgg atagggcatg
     3721 atcagccgcc cgtccggggc ggcggtgaag ctggcctggc tcggataggc gaactggatg
     3781 ccgagctcgg cgaagcggcg caggatgtcg agcatcaccg cctgccgcgc ctcgaggaaa
     3841 tccgcgagct ggtgcgaatc gacgaagaag acgagctcgt agtcgagcga ggacggcgcg
     3901 aatttgacga ggtgggcgcg atcgaacctg gcgtgcttcc ggcgccggac gatcgcctcc
     3961 agctcggcgg gcaccgtctc gagcagctcc ggcggcgtct ggtagaccag gctgagggcg
     4021 accagcgcgc gccgccgctc cagccgggtc cagttctgga gccgcgtgtt gagcaggttg
     4081 gcgttcgaga cgatgagctc ctcgccgccg accgagcgga tgcgggtgct cttcagcccg
     4141 atccgctcga ccgtgccggt gtgcgtgtcg aagctgatcg tgtcgccgcg ccggaacggc
     4201 ttgtcgaaca ggatcgacag cgccgcgaac aggtcggaga agatgccctg cgccgcgagg
     4261 ccgatggcga ttccgccgat gccgaggccg gcgaccaggc cggtgacgtt gactccgaga
     4321 ttgtcgagga tgaggatgac ggcgatcgcg aacagggtca ccgtcaccag cagccggatg
     4381 atcccgatcg ccgaggctag gttgctctgg tcctgctcgc cgccggtgcg atgggcgacg
     4441 tagccgagga tcagctcgcg cgcccagatc gccgcctgca gggcggcggc gatgacgaag
     4501 ccgacatgga tggcgttgag cagggccggc ggggtgttgg catgctcggc gaccagctcg
     4561 gccgaggtga agacgatgaa atagagcttg gtcttggcga gggcgcgcgc gaagatcgcc
     4621 ggccagtgcg gatcggtgcc gagcctcgcg atcagcctgc agcccagcga gcggacggcg
     4681 agcatggcga gcgcgatccc gatcccgatc gccgccgcga ccaggatccc ggcccagttg
     4741 ccgccgaccc aggcctgcgc ctcggccgcg agcgcgctgc tcttcgagag gaaattgccc
     4801 gtcgcgccgt ccgccatcag gccgcctttg ccgcagcttc cgccttctcg tccagcgcct
     4861 cgagaaattc gatcagcccg cgctcggcgg agctgagata tttggacgcc cgcggcagct
     4921 tgcgctcgcc gatcgcgccg gcgtcgcggg cggcgtcgat cacggccggg tggacgtagg
     4981 atttgcggct gatcgcggcg gtgttgccga gggcctcggc gaccggctcg atcacgctct
     5041 tgaggttcac cttgccgtgc ttgcggtagc ggcggatcat ctcctcgaag gcgatggtgc
     5101 ttgcgcccca ggtgcggaag tccttggcgg tgaaatcggc gcccatcgcc tccctgatgt
     5161 aggcgttgac gtcggtcgag gtgaccggac aattgtcgcc gtcgtcgttc acatattcga
     5221 acagattctg gccgggcaga tcctgggtct tgcgggcgat gcgcaccagg ttgcgatcgg
     5281 tgagggtgac ggtgcgcttg atgccgtgct tgccggtata gctgatcttc agccggccgg
     5341 gctcgacctt ggcgtggcgg ttgcgaagcg tggtggctcc gaagctcttg ttgtccttgg
     5401 catattcctc gttgcccacg cgcatgcggg tctcgtcgat cagccgcacc acggccgcca
     5461 gcaccgtatc ccgagcggtc ggcttgccct ggagatcctc ctcgacccgc tttcgaagct
     5521 tcggaagcgc ctgtccgaag gcggcgagcc gctcatattt ggcagcctcc tgccgggccc
     5581 ggaactcggc gtggtagcga tattgcttgc gcccgcgcgc gtcgtagccg gtcgcctgga
     5641 cgtggccgtt gggcttcggg cagaaccagc agcgctcgta ggccggcggc atgccgaccg
     5701 cattcagtcg gtcgatctcg tcgcgatcgg tgatccggct gccgtcgggc gcgaaatatt
     5761 gccagtagcg gccgcgccgc ctgcgggtga ttccgggcaa ggtccgatcg tcgacgaagc
     5821 agagcatggg aagcgctaag cgccccgcgc cgccggttcg ttccgggaac gcgttaccgt
     5881 cctcgccgtt gattgagaaa aggagggtgc catgccgaac atcgccgatg cgaagatact
     5941 gatgatcgcg accgacggct tcgaggacag cgagctgatc gagcccaggc gtatcctgcg
     6001 cgagcggggc gccgacgtca ccgtcgcgtc gctcgagaag cgcgagatca ggggcagcca
     6061 cggaacgagc gtcgaggccg acacggccat cggcgaggtc gatgcggacg gctacgacgc
     6121 gctcgtcctc cccggcggca ccaggaatgc ggacaagctg agggacgacg agcgggtggt
     6181 ggcggcggtc cgggccttcg ccgaagccgg caagccggtc gccgccatct gccacgggcc
     6241 ctggctgctc gtcgaggcgg acgtgatccg cgggcgcacg gtcaccgcct ggtcgtcgat
     6301 ccacaccgac ctgcgcaacg ccggcgccga cgtgatcgac caggaggtcg tgatcgacgg
     6361 caacatcgtc accagccgca agcccgacga cattcctgcc ttcaccgagg cgctgatcgt
     6421 cctctacgag aaggcggacg cgcccctcga gaccgtttga gggccggcca ggaacggctt
     6481 cctggcggcc ccgcgggcgc ggcgcgcgac ataggcgtcg aggccgatgg cgacggcgaa
     6541 caccagccac ggatagggct ggtaggcgat tccgacgaac agcgagccga tcaggtagat
     6601 gatctggaaa tgctggagcg ccgtcgccag cggcgcgatc caggcgtcgt cgccctcggc
     6661 cttgcggtag cggcggcgga ggatctcgag tcggaccagg cccgcgccat ggatcataag
     6721 gaacaggatg aggccgggga agccctgctc gcccagcatc tcgaaatagg aattatgata
     6781 ggcccggccc tggtccatct cggtccgggt cgtcaccgat tcgacgccgc cgacgacctg
     6841 ggtcgagacg gtccgcacct ggatcttgtt ctggcgatag gcgccgaagc cgccgccgag
     6901 cgggtgctcc tgcacatagt cccaggtcca cgcccagacg gcgaggcgga cgttggccga
     6961 ggaatcggat tcgtagccct ggatggtcga catccggccg gtgaaggagg cgggcaggaa
     7021 ggggatggac gcggcgcaga gcagggccgc gcccgtgagg tagaggatcc gccgcttgac
     7081 atcgcgaagc atgaggacgc ccagcacgcc gatgcagacc agcccggtgc gcgcctcggt
     7141 cccgatcggc atcagcaggc aggcgaagat caaggcggcg gcgaaggtct tgacccgcca
     7201 gtcgggcggg aacaccgtcc cgaaccgcgc cagccacagg atcatcggga tgatcgcgat
     7261 cgccacggtc gaaatcgtgc tgctctcgta gaggttgctg ttgttgttca ccatcaggtt
     7321 gaggacgcca tagccgcctc ccgacagcac cgtcttgatc ccgccgacga tgatgatcgc
     7381 ggcggccgac aggatcaggt agaggatcgt cgcctcgatc cgaagcttcg agcggagggt
     7441 cagcggaagg aagatcgcga aggcgagcac cttccaggcc cagtcccatt tctcggcggc
     7501 ctcgatcggg aagtccgcat tcctcgtcgt gtagaaggtg tagcagagca ggatggcgat
     7561 caggccctgc cggacgctga accgcatctc ggccttcttc tcggccacca gccagccgac
     7621 cagcgcgagg caggcggcga tcatcgacac cgggacgctg ttgatcaggt aataggacag
     7681 gcgctgcggc gagacgacgt cgatatagat gtaggcgagc acgaacagga acggccgccg
     7741 caggccgagc ccgaacaggg cggccaggaa gaggatgaag gcgaggtcac gcatcgccgg
     7801 cccctttgcc ggggggcgga gccggcgcgg cgggcgccga gtcatcgtcg agatcgggcc
     7861 gcatcaggat gcgccaggcg acgatcagga gtaggacatg ggtcaaagcc agggagaaat
     7921 tgtcgatcat ccagccgtcc ctgcgcccgc ctcttccagc gcttcttaga gggacgcggt
     7981 tgacgggccg ttaagcttga ggtggcagag cggagggcga tgggcgccct cttcctcgtc
     8041 cggtccgccg accccgacgc cgccgactcg gcgatggcgc gggcgcgcgt gcagttcggc
     8101 cgccacggcc tggccggcct cgccgaaaga gccttccccg gctggcggct gcttcacgcc
     8161 ccccacatcc tgggcgggcc cgaaagcctg ctggaggcgg gcgaggactt cgtcgccgtc
     8221 gccggcaccc tgacctggga cgacatgatg gggcggcccg cgctggaggc cctgctccgg
     8281 accgtctcgc tgccggagcc ggactggtcc cggctcggcg gccagttcgc cgctctcttc
     8341 cgccggcagg ggcgcgcctt cctgctcacc gaccatttcg ccatgttc
//
LOCUS       DASVQZ010000366         7433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_847241, whole genome shotgun sequence.
ACCESSION   DASVQZ010000366 DASVQZ010000000
VERSION     DASVQZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847241"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VF547_10375"
     CDS             <1..1915
                     /locus_tag="VF547_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263265.1"
                     /translation="VRRYPYETAALPMTRQYVASWTIRDRYIVSWDPATWKGRLEKDA
                     AETEAVLAAVLARNPPEKVTAPSVLIVSVQQPAKDAGAAPPPPLLYRFELAPPSTPGD
                     RPTLYGQFIASGAASQPLEAPAAPTGYKPGKLEGYLSGFPAQGKDAYFAAYPQEHAQL
                     ASFIDKAPAAVDQLVRTATTTGAVTTHQSLFRVIRTVDGQGKAVLDVTLLSEPTPGGV
                     PAAVFRLSYSTDLQLEELRAASRPEADRLGAVTLPDVLAAEERTAVNLAIYRYFAGRG
                     VRMREVDARLPVGGGERFVLFTLRFGAGNDVTVERLGEAGTGPGQIDTGRIDVTRANG
                     FPGAASSDSALRGWWKTRYRKGGTLKTKAPSEPAKVPGTADAVAAAADNVPLISEMNA
                     LIAAGCVKSAWFRDNYGVTVMGAEAAKARMISAHGATAADVRDSTAEKGEPPKAKGPL
                     ADMKDFSTGELTGLENAYQTLGTGDLGRLSGVSVGRKGTGVERKDGKWVFASYSGYAM
                     KDGDKRTILYFDSMHLRDDHMFLGGSAANALPAFTMTILHETGHVTEYTGGIRAAFNA
                     WLKDNPQEPPTWYSKDKPKTELFPEAYALFHADPKFLCASAPLMYAWFLELSRKGTAP
                     AANAKLKPPPCPG"
     gene            complement(2070..2447)
                     /locus_tag="VF547_10380"
     CDS             complement(2070..2447)
                     /locus_tag="VF547_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8263266.1"
                     /translation="MVRILLAEDDASMRAYLGRALERAGYEVVAVDRGTAALPLIAAD
                     SFDLLLTDIVMPEMDGIELAQKAALVAPEMRVMFITGFAAVALKAGKAAPSAKVLSKP
                     FHLRDLVAEVDRLFQTEDQHGRL"
     gene            2607..3464
                     /locus_tag="VF547_10385"
     CDS             2607..3464
                     /locus_tag="VF547_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX8263267.1"
                     /translation="MLDSDPPFRRLGPARPGSPVVLSVPHAGRAYSDRLLAAARLSRA
                     RLESLEDRLVDRLVWRAVEAGAVALIADSPRAEIDLNRDERELDPAMVLPRPPAEATV
                     ESPRTRGGLGLIPARIAGSGAIWRQRIAAAEVSRRIERIHRPYHEAIEAELRAAKARF
                     GVAVLLDCHSMPPRGAGGEAPVVLGDRHGDSMAEALVTAAERAVREAGFKVARNAPYA
                     GGHITERHGRPVRGIHALQLELDRSLYLAPDLRSSGPGFDRTARLIAAVAQALAAAAL
                     DPPEAVAAE"
     gene            complement(3796..4359)
                     /locus_tag="VF547_10390"
     CDS             complement(3796..4359)
                     /locus_tag="VF547_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX8263268.1"
                     /translation="MATAAPDLARYSRVAAWLHWIIAALIVVNLVLGFFHEDFERPVR
                     SAMMSLHKATGLTILALTLARVAWRLGNRPPPFDPVMKRWEVGVARAVHWSFYLLLIV
                     LPLSGWLVVSTSGRATSWFGLFEVAPLPVTRAEDAHELMEEVHEILAYAMLALLALHV
                     LGAVKHHVEGHRHLIGRMALWSRRGGP"
     gene            4480..4824
                     /locus_tag="VF547_10395"
     CDS             4480..4824
                     /locus_tag="VF547_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8263269.1"
                     /translation="MASGASREKKSLTRLSAELERLAGELVRLGGAPPAPAAQAGERP
                     SDRLRRRLHEARRARGMRQADLAERLGRPQSFVSNYERGTRRLEVSEFIQIARILALD
                     AAAVVAELAAES"
     gene            complement(4837..5622)
                     /locus_tag="VF547_10400"
     CDS             complement(4837..5622)
                     /locus_tag="VF547_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX8263270.1"
                     /translation="MATEPPSNPLPIFYGGLEPLSSSVHADYRSKSSDVAPFLVHQHA
                     VPITVDEFVAVQRFYPLVFSVGENPVPLALMGLNENVNVFVDADGRVLGDIYLPAYVR
                     RYPFMLARLSPDAQELSLCFDPTSGLVGNLSEGELLFDDGKPSEATNNILKFCEEFEL
                     SAQRTVAFMQDLKAGDLLMDGEVSIQPAGAEQPFVYRGFQMVNEEKLRELTPDQLERM
                     NKNGMLPLIYAHLFSMSLVREIFARQMQQGKGPGAAAPAVGNA"
     gene            5774..6214
                     /locus_tag="VF547_10405"
     CDS             5774..6214
                     /locus_tag="VF547_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263271.1"
                     /translation="MEGIPIRAAAAVLALLVAAAPAAAPSQRPAQYGQGIVREQVVIR
                     FRQSAPARIEWKEGKGPRCIPTRGILAATRAVKDSVDLILRNRQRVRAKLESSCPALD
                     YYYGFYIRPDPDGMICEDRDVIRSRMGGACEIDRFRSLEPVAAR"
     gene            6299..>7433
                     /locus_tag="VF547_10410"
     CDS             6299..>7433
                     /locus_tag="VF547_10410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8263272.1"
                     /translation="MSFADLGLSDELLRAVGESGYTEPTPIQTQAIPSVLMGRDLIGI
                     AQTGTGKTAAFVLPMIDILGEGRSRARMPRSLILEPTRELAMQVSENFEKYGKYHKLN
                     MALLIGGTNMADQIKKLEKGVDVLIATPGRLMDLFGRGKIMLNGCSLLVIDEADRMLD
                     MGFIPDIEEICSKLPAVRQTLLFSATMPGPIKKLADKFLTGPKTIEVARPATANVSID
                     QRVLKVDSRRKKQVLTGRLKGHDVRTAIVFCNRKTTVRELAQSLKRSGLNASEIHGDM
                     EQPERIRELERFKAGEINILVASDVAARGLDIKDVSHVFNYDVPWHPDDYIHRIGRTG
                     RAGKTGIAITLATAADAEAIEAIEKLTGAKIQPVEATNSSSQAA"
BASE COUNT         1289 a         2606 c         2467 g         1071 t
ORIGIN      
        1 cgtccgtcgc tatccctatg agacggcggc tctgccgatg acgcgccaat atgtcgcatc
       61 ctggaccatc agggatcgct atattgtgtc ttgggacccg gcgacctgga agggacgcct
      121 ggaaaaggat gccgccgaga cggaggccgt cctggcggcg gtgctggcgc gcaatccgcc
      181 ggagaaggtc acggctccga gcgtcctcat cgtctccgtc cagcagcccg caaaggacgc
      241 cggcgccgcg ccgccgcccc cgctcctcta ccgtttcgag ctggcccctc cctcgacgcc
      301 gggagaccgg cccacgctct acggccaatt catcgcgtcg ggcgccgcca gccagccgct
      361 cgaggcgccc gccgccccga ccggctacaa gccgggaaag ctcgaaggct atctgtccgg
      421 cttcccggcg cagggcaagg acgcctattt cgccgcctat ccgcaggagc atgcgcaact
      481 ggcgagcttt atcgacaagg cgccggcagc ggtcgaccag ctcgtcagga ccgcgacgac
      541 gaccggggcc gtcaccacgc accagtccct cttccgagtc atccgcaccg tcgacggcca
      601 aggcaaggcc gtcctcgacg tcaccctgct gagcgagccg actccgggcg gagtgcccgc
      661 cgccgtcttt cgcttaagct acagcaccga tctccagctc gaggagcttc gcgcggcttc
      721 ccgtccggaa gcggatcggc tcggggccgt aacccttccc gacgtcctcg cggccgagga
      781 gcggaccgcc gtcaacctcg ccatctaccg gtattttgcc ggtcgcggcg tacgaatgag
      841 ggaggtcgat gcccggctcc ccgtcggcgg gggcgagcgc ttcgtgctgt tcaccttgcg
      901 cttcggggcc ggaaacgacg tcaccgtcga gcgtctcggc gaggctggaa cgggcccggg
      961 ccagatcgac acggggcgga tcgacgtgac tcgggcgaac ggctttccag gagcggcctc
     1021 gtccgactcg gcgcttcgcg gctggtggaa gacccgctat cgcaaggggg gaacgctcaa
     1081 gacaaaagct ccctcggaac cggccaaggt cccaggaacg gcggatgccg ttgccgccgc
     1141 ggccgacaat gtcccgctga tcagcgaaat gaatgcgctg atcgccgccg gatgcgtgaa
     1201 gtcggcatgg ttcagggaca attacggcgt gaccgtgatg ggagccgagg cggcgaaggc
     1261 tcggatgata agtgcccacg gggcgacggc cgccgacgtc agggactcga cagccgagaa
     1321 gggcgagccg cccaaggcca agggcccgct cgccgatatg aaggactttt cgaccggcga
     1381 gctcacgggc ctcgaaaatg cctaccagac cctgggcacg ggcgatctcg ggcgcctctc
     1441 gggggtcagc gtcggacgga agggcacggg cgtcgagcgc aaggatggaa aatgggtatt
     1501 cgccagctat tccggctacg cgatgaagga cggggacaag cgcaccatcc tctacttcga
     1561 ctccatgcat ctcagggacg accacatgtt cctgggcgga agcgccgcca acgccctgcc
     1621 cgcgttcacc atgacgatcc tccacgaaac aggccatgtg acggaatata ccggtggcat
     1681 ccgggcggcc ttcaacgcct ggctgaaaga caatccgcag gagccgccga cctggtattc
     1741 caaggacaag ccgaagacgg agctgtttcc cgaagcctat gcgctgttcc acgccgaccc
     1801 gaagttcctc tgcgccagcg caccgctgat gtacgcctgg ttcctcgagc tttcgcgcaa
     1861 gggcaccgcc cccgcggcca acgccaagct gaagccgccc ccctgtcccg gctgagccgg
     1921 ctcctaaagc ggtggtcagt cagatgcgct atctccgttc gggctgagct tgtcgaagcc
     1981 ggttcctttt ctctcatgga acaacaagga aagcccttcg acaggctcag ggcgaacggg
     2041 gagagtgatt cacctaatcc ctcaatgcgc taaagccggc cgtgctggtc ctcggtctgg
     2101 aacaggcggt cgacctcggc gaccaggtcg cgcaggtgga agggcttgga cagcaccttg
     2161 gcgctcggcg ccgccttgcc ggccttcaga gccaccgcgg cgaagccggt gatgaacatc
     2221 accctcatct ccggcgccac cagggccgcc ttctgggcca gctcgatccc gtccatctcg
     2281 ggcatgacga tgtcggtcag cagcaggtcg aagctgtcgg ccgcgatcag cggcagtgcg
     2341 gcggtgccgc ggtcgaccgc gaccacctcg tagccggcgc gctcgagcgc gcggccgaga
     2401 taggcccgca tcgacgcatc gtcctccgcc agcaggatcc tgaccatgca tgcccctgga
     2461 accggccgcg cccgatgcgc gtccgccgcc gcccttctat gcccgaaacg aaccgccgtt
     2521 tccagcgcgc ccgcgccgga aggcgccctg cgcgcggcgg aagacgattg cgtgccaaat
     2581 ccggactact tacccttgag gtgcaaatgc tcgattccga cccccctttc cgccgcctcg
     2641 gaccggcccg gcccggctcg ccggtcgtgc tgtcggtgcc gcatgcgggc cgcgcctatt
     2701 cggatcggct gctcgccgcg gcccgcctct cgcgcgcccg gctcgagagc ctcgaggacc
     2761 gtctggtcga ccggctggtg tggcgggcgg tcgaggccgg cgcggtcgcc ctgatcgccg
     2821 actcgccgcg cgccgagatc gacctcaacc gcgacgagcg cgagctcgat ccggcgatgg
     2881 tgctgccgcg cccgcccgcc gaggcgacgg tcgaatcgcc gcgcacccgc ggcgggctcg
     2941 gactcatccc ggcccggatc gcgggctccg gcgcgatctg gcgccagcgc atcgccgccg
     3001 cggaggtcag ccggcggatc gagcggatcc accgccccta tcacgaggcg atcgaggcgg
     3061 agcttcgagc ggccaaggcg cgcttcggcg tcgccgtcct gctcgactgc cactcgatgc
     3121 cgccgcgcgg agcgggcggc gaagcgccgg tcgtgctcgg cgaccgccac ggcgacagca
     3181 tggcggaggc cctggtgacg gcggcggaaa gggccgtgcg cgaggccggt ttcaaggtgg
     3241 cccgcaacgc gccctacgcc ggcggccaca tcacggagcg ccacggccgc ccggtccgcg
     3301 gaatccacgc cctccagctc gagctcgacc gctccctcta tctggcgccg gacctgcgca
     3361 gctccgggcc gggcttcgac cgcaccgccc ggctgatcgc cgccgtcgcg caggccctcg
     3421 ccgccgccgc cctcgacccg cccgaagcgg tcgccgcgga gtgaatgccg ccggtcggga
     3481 cgctggagca tcgagggatt aggccggatc actctcaccc gttcgcccct gtcgttctct
     3541 aagagaaaag gagacggctt cgacaagctc agcccgaacg aagagagcgc gatccgaccg
     3601 ccgagcgctc caagaggccc aactcgccgc cgccggatcg cggccccaat cgcctccccc
     3661 catacgaaaa gaccaccccg cgggatcgcg gggtggccag gttcagggag gagggccccg
     3721 aagggcccaa cgcgccgccg caaaggggga aatacgtcgg cgcgcaatct aaatgtatga
     3781 aagccttgga aggtttcaag gtccgccgcg gcggctccaa agcgccatcc gtccgatcag
     3841 atgccgatgc ccttcgacat gatgcttgac cgcgcccagc acgtggaggg cgagcagcgc
     3901 cagcatcgca taggcgagga tctcgtgcac ctcctccatc agctcgtgcg cgtcctcggc
     3961 gcgagtcacc ggcagcggag cgacctcgaa caggccgaac cagctggtgg cgcggccgct
     4021 cgtcgaaacg accagccagc cgctcaacgg cagcacgatc agcagcagat agaagctcca
     4081 gtggacggcg cgtgcaacgc ccacttccca tcgcttcatg accggatcga agggcggcgg
     4141 ccggttgccc agccgccagg cgacccgagc gagcgtgagg gcgaggatgg tgagaccggt
     4201 cgccttgtga aggctcatca tcgccgagcg caccggcctc tcgaaatcct cgtggaagaa
     4261 gccgagcacc aggttgacga cgatgagcgc ggcgatgatc cagtgcagcc aggcggcaac
     4321 tcgggaatag cgggcaaggt cgggagcagc ggtggccatg ccgaatcgct acagcgcgcc
     4381 gggcggcacc gcaacggccg gatcgcggaa ttcggccgcc tctgaaaaat gcgcgaaaaa
     4441 cgactattca acgccgattg cttgcgctaa acgcgaggaa tggcaagtgg ggcaagccgg
     4501 gaaaagaaga gcctgacgcg gctcagcgcc gagctcgagc ggctggcggg ggaactcgtg
     4561 cgcctgggcg gcgcgccgcc ggccccggcc gcccaggcgg gggagcgtcc ctccgatcgc
     4621 ctgcgccggc ggctgcacga agcgcggcgg gcgcggggca tgcgccaggc cgacctcgcc
     4681 gagcggctcg gccgcccgca atccttcgtc tccaactacg agcggggcac gcgccggctc
     4741 gaagtctccg aattcatcca gatcgcccgg atcctcgccc tcgacgccgc agcggtcgtc
     4801 gccgagctcg ccgcggagag ctgagcgaaa agggccctag gcgttgccca ccgccggcgc
     4861 ggcggcgccg ggacccttgc cctgctgcat ctggcgggcg aagatctcgc ggaccagcga
     4921 catggagaag aggtgggcat agatcagcgg cagcatgccg ttcttgttca tgcgttcgag
     4981 ctggtcgggg gtgagttcgc gaagtttctc ttcattgacc atctggaagc cgcgatagac
     5041 gaacggctgc tcggcgccgg ccggctggat cgacacctcg ccgtccatca gcaggtcgcc
     5101 ggccttgagg tcctgcatga aggcgaccgt gcgctgcgcc gaaagctcga actcctcgca
     5161 gaacttgagg atgttgttgg tcgcctcgct cggcttgccg tcgtcgaaca gcagctcgcc
     5221 ctcggacaga ttgccgacca ggccgctggt cggatcgaag cagagcgaca gctcctgcgc
     5281 gtccggcgaa agccgcgcga gcatgaacgg atagcggcgg acataggcgg gcaggtagat
     5341 gtcgccgagg acccgcccgt cggcgtcgac gaacacgttg acgttctcgt tgaggcccat
     5401 cagcgccagc ggaaccggat tctcgccgac cgagaagacc aggggataga aacgctggac
     5461 ggcgacgaac tcgtcgaccg tgatcggcac cgcatgctgg tggacgagga acggggccac
     5521 gtcggaggac ttgctccgat aatcggcatg gacgctgctc gacagcggct cgaggccacc
     5581 atagaaaatc ggcagggggt tcgacggcgg ctcggtcgcc atgttgtgtc tccgctagcg
     5641 ttggaagcgg ccgcgtcttg ggccgctcga atgtggcggc ggtttctatg gcgcaatcgc
     5701 ggctttcgca agcgggtaag ccgggccggg gattaatcgc ctgttcaagc gtttgcgggg
     5761 aagggacgaa gcgatggagg gcatcccgat ccgtgcggcc gcggccgtcc tggccttgct
     5821 cgtcgcggcg gcgccggcgg cggcgccctc gcagcgcccg gcccaatatg gccagggcat
     5881 cgtccgcgag caggtcgtca tccgcttccg ccagtcggct cccgcgcgca tcgagtggaa
     5941 ggagggcaag ggcccccgat gcataccgac tcgcggcatc ctcgccgcca cccgagcggt
     6001 caaggacagc gtcgatctga tcctccgcaa ccgccagcgg gtccgggcca agctcgagag
     6061 cagctgcccg gcgctcgatt attattacgg cttctacatc cggcccgacc ccgacgggat
     6121 gatctgcgag gatcgcgacg tcatccgctc gcgcatgggc ggcgcctgcg agatcgaccg
     6181 tttccgcagc ctcgagccgg tggcggcacg ctgagctttc ttgacttggg ccgccgcttc
     6241 gcccaagcgc tggcctgaag cggctcgcca cgaagcccct caactacccg gaacccttat
     6301 gagttttgcc gacctcggcc tttccgacga attgctccgc gccgtggggg aatccggcta
     6361 caccgagccc acgccgatcc agacccaggc gatcccctcg gtgctgatgg ggcgcgacct
     6421 gatcggcatc gcccagaccg gcaccggcaa gaccgccgcc ttcgtgctgc cgatgatcga
     6481 catcctcggc gagggccgga gccgggcgcg gatgccccgc tcgctgatcc tcgagccgac
     6541 ccgcgagctg gcgatgcagg tctcggagaa tttcgagaaa tacggcaagt accacaagct
     6601 caacatggcc ctgctgatcg gcggcaccaa catggcagac cagatcaaga agctggagaa
     6661 gggcgtcgac gtcctgatcg ccactccggg ccggctgatg gacctgttcg ggcgcggcaa
     6721 gatcatgctc aacggctgca gcctgctcgt catcgacgag gcggaccgga tgctcgacat
     6781 gggcttcatc cccgacatcg aggagatctg ctccaagctc ccggcggttc gccagaccct
     6841 gctcttctcc gcgacgatgc cgggcccgat caagaagctc gccgacaagt tcctgaccgg
     6901 ccccaagacg atcgaggtcg cccggccggc gaccgccaac gtctcgatcg accagcgcgt
     6961 gctcaaggtc gattcgcgcc gcaagaagca ggtgctgacc ggtcggctca agggccatga
     7021 cgtgcgcacc gcgatcgtct tctgcaaccg caagacgacg gtccgcgagc tcgcccagag
     7081 cctcaagcgc tctggcctca acgccagcga gatccacggc gacatggagc agcccgagcg
     7141 catccgcgag ctggagcgct tcaaggccgg cgagatcaac atcctcgtcg cctccgacgt
     7201 cgccgcccgg ggcctcgaca tcaaggacgt cagccacgtc ttcaactacg acgtgccctg
     7261 gcatccggac gactacatcc accgcatcgg ccgcaccggc cgggccggca agaccgggat
     7321 cgcgatcacc ctcgccaccg ccgcggacgc cgaggcgatc gaggcgatcg agaagctgac
     7381 cggagcgaag atccagccgg tcgaggcgac gaactcgtcg tcgcaggccg ccg
//
LOCUS       DASVQZ010000367         6952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_368649, whole genome shotgun sequence.
ACCESSION   DASVQZ010000367 DASVQZ010000000
VERSION     DASVQZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368649"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..477)
                     /locus_tag="VF547_10415"
     CDS             complement(97..477)
                     /locus_tag="VF547_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8263273.1"
                     /translation="MLKSILTLVAAALGADAADSAPTGEPARARANLTALFSDADYPA
                     AAIAAREQGTVEFMLEVAATGLVQGCTVTGSSGSEALDSATCGVLAQRARFTPARGPG
                     GIPVPDRVKGRVRWLLPAAPPPAG"
     gene            complement(552..1241)
                     /locus_tag="VF547_10420"
     CDS             complement(552..1241)
                     /locus_tag="VF547_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEX8263274.1"
                     /translation="MNYSRRQLLGAGLSAGFLATAAQGQTLAEIVAGLKDPVPTPPPA
                     RPLTVPARPRVTVDGVIDPRLLARARASFDRHRSQLRFTDVVGIADFSRPSHDHRFFL
                     LDTASGRVSNHLVAHGRGSDPAHRGFLERFSNQIGSEASSQGGYRTGEYYHGKYGRSL
                     RLAGLDWSNSNAEARAIVVHSAWYAEPDQIAAHGKLGRSEGCFALPYNSLQQVLARLG
                     PGRFLYADKLA"
     gene            1431..2477
                     /locus_tag="VF547_10425"
     CDS             1431..2477
                     /locus_tag="VF547_10425"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX8263275.1"
                     /translation="MAEFDRLAVKPGLPPMEAKLVDALPEGEGWQYEPKWDGFRCLVF
                     RDGPDVALMSKSGKPLGRYFPEVVDTVLGMEADRVVLDGELIIPVDGALSFDALQLRL
                     HPAESRVARLSRETPAQLMLFDLLFSKETSYLEAPLELRRRSLEIIYAAEQHDDLLLS
                     PCTTELEAARRWLEEVGAELDGVVAKRIDAPYQPGERAMLKIKAMRTADCVVGGFRYA
                     TNSREVGSLLLGLYDEAGLLHHVGFTSSIPAAERAELTRRLEALVEAPGFTGDSPGGP
                     SRWSTERTGEWQPLRSELVAEVRYDHVTGRRFRHGTRFLRWRPDKAPEQCTMEQLHAE
                     APPEALAARLAAAS"
     gene            2474..3355
                     /gene="ligD"
                     /locus_tag="VF547_10430"
     CDS             2474..3355
                     /gene="ligD"
                     /locus_tag="VF547_10430"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8263276.1"
                     /translation="MTAEGFVAGVRLSNPDKVLYPEQGITKGQLADYYVAVADRMLPH
                     VGFRPVTMVRCPMGQETKCFYQRHAGSGVPEQLREVAIPGFDEPYLYICDVSGLVAMV
                     QMGVLEIHPWGVRVEAPDRADRIVFDLDPAEGLGFEAVIEAAREVRQRLKALGLTSFV
                     KTTGGKGLHVVVPVEATAPWKEVKSFAKGLSAEMAADSPDKYLTTAGKADRVGKIFID
                     YLRNDPTSTAVAPYSTRSRKGAPVAMPIRWEQVKAGLDPCDYTVETVPALLRRQRSDP
                     WAEMLEVRQTLPDGPRA"
     gene            3437..3988
                     /gene="msrA"
                     /locus_tag="VF547_10435"
     CDS             3437..3988
                     /gene="msrA"
                     /locus_tag="VF547_10435"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8263277.1"
                     /translation="MAEQQAILAGGCFWCTEAVFNDVIGVTRVESGYIGGNVANPTYK
                     QVCGGDTGHAEAIRVTFDPEIISYDELLDLFFATHDPTQLNRQGNDVGTQYRSALFPL
                     SAEQEAAANRAIERARADWPGPIVTTIEPVGDWWPAEDYHQDYWAGEGQRNPYCLAVI
                     PPKLKKLRKSFAHRVKSEAAVSE"
     gene            4086..4463
                     /locus_tag="VF547_10440"
     CDS             4086..4463
                     /locus_tag="VF547_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX8263278.1"
                     /translation="MNMMKWTALAVVAAMAAAQPAVAKKARTYIVNNEAVGVPVFPHD
                     ITDRPYTVIGEVKAGVRKATVFSKAASQKKIYRELWERARKKGADAVINASYGDSKIS
                     AMSWGQTNARGTAIRFTTPPASN"
     gene            4495..5103
                     /locus_tag="VF547_10445"
     CDS             4495..5103
                     /locus_tag="VF547_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263279.1"
                     /translation="MPVRLIALALLASASGAAAQEAASAAAQPDLVVATPAAPADRPG
                     AIVVARDTMVRLMVLNEVNTNSAKPGDRFVLRVDENVVVGGSTVIPVGAKAYGEVTRV
                     KSNGAVGKAGSVGAKLLYVELGEDRVPLIGESESKGRKEGGGVVMASAIWGPFGLLMK
                     GGAGKLKAGHIFNGYIPADLLYDPASGRFIDPEAKAEAAAAP"
     gene            5100..5612
                     /locus_tag="VF547_10450"
     CDS             5100..5612
                     /locus_tag="VF547_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263280.1"
                     /translation="MKHGRALLAAVLLIAAPVGAGAQPAPGPVLLKGTPVVLKTLAPL
                     SSRRARQGQRFDVEVSDEIRVDGLLVIPRGARGVGEVSRVVTKGMVGKPGKLEVRVLY
                     VEVAGRRIRLDGVASDKGASGAAPVVLAAPLIGVSAAFFTGKSAVLPAGSTIEGFVYQ
                     DMPLVRPSGD"
     gene            5661..6107
                     /locus_tag="VF547_10455"
     CDS             5661..6107
                     /locus_tag="VF547_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263281.1"
                     /translation="MNRASGALLGGLLAAAAWAPAPADARTARGCAISLTAAADGSFA
                     QAGYNSGPARLAKLAAATRERFTAAALRLCALKVLTPGDLARFGRLVVQNGEGATEPL
                     LYQDPAMGPRVFIFQFAFQDGGPPEPAEFEQALRCWKRPKRCDFGD"
     gene            6309..>6952
                     /locus_tag="VF547_10460"
     CDS             6309..>6952
                     /locus_tag="VF547_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263282.1"
                     /translation="MKNIHRALLVSASLTIVPVAAAAQIAPVAQPAVSPIIAPAPTSA
                     VLRVGTEVPLRLREELTTKGKKIRTGYRFQLETSEPVMVQGVTVIPPGSPAVGEVTSA
                     RNKGMWGKSGKLNARILYVMVNGRQIRLTGNFDDKGTAGGIAAVAVSAVVFLPAGFFM
                     TGTSAKVPAGTIVKAFVDEDVPLALAAAPVQPLTVAAPAAVMTVAAPVPAAVAPA"
BASE COUNT         1071 a         2343 c         2487 g         1051 t
ORIGIN      
        1 ccgggcggct tctacgtcaa cgtccacacc ccggattatc cgaacggcgc gatccgcggc
       61 cagctgatca acgtcgagaa ggacggcctg taaggttcag ccggcgggcg gcggggccgc
      121 cggcagaagc cagcgcaccc tgcccttcac gcggtccgga accgggattc cgcccggacc
      181 gcgcgcggga gtgaagcggg cccgctgggc gaggacgccg caggtcgcgg agtcgagcgc
      241 ttcggagccg ctcgaaccgg tgacggtgca gccctggacc aggccggtcg ccgcgacctc
      301 gagcatgaac tccaccgtcc cctgctcgcg ggcggcgatc gcggcggcgg gatagtccgc
      361 gtcggagaac agcgcggtca gattggccct tgcgcgcgcc ggctcgccgg tcggcgcgga
      421 gtcggcggcg tcggcgccga gcgccgccgc cacgagcgtc aggatggatt tgagcatggc
      481 tggtcccccc ggattgtccg cgggaccttt tcacattccc cgcccgaggg cgatctgcgg
      541 gaattaccgg ctcaggcgag cttgtcggcg tagaggaagc ggccgggccc cagtcgggcg
      601 aggacctgct gcaggctgtt gtaggggagg gcgaagcagc cctccgaacg gccgagcttg
      661 ccgtgggcgg cgatctggtc gggctcggca taccaggcgc tgtggacgac gatggcgcgc
      721 gcctcggcgt tggaattgct ccagtcgagc ccggcgaggc gcagcgagcg gccgtacttg
      781 ccgtgataat attcgccggt gcggtagccg ccctgcgacg aggcctcgga gccgatctgg
      841 ttggagaagc gctcgaggaa gccgcgatgg gccggatccg agccgcggcc gtgggcgacg
      901 aggtggttgc tgacccggcc ggaggccgtg tcgagcagga agaagcggtg gtcgtgggag
      961 ggccggctga agtcggcgat gccgacgacg tcggtgaagc gaagctgcga gcggtggcgg
     1021 tcgaacgagg cgcgggcgcg ggcgaggagg cgcgggtcga tcactccgtc gacggtgacc
     1081 cgaggccggg cgggaacggt cagcggccgg gccggcggcg gagtcggcac cggatccttc
     1141 agcccggcga cgatctcggc cagggtctgg ccctgggccg ccgtggcgag aaaaccggcc
     1201 gaaagaccgg ccccaagcaa ttgcctgcgc gagtagttca agattttccc ctcagctctg
     1261 acaacctagt tatagctttt gtccttaagt tccagtgaac tgcagccgct tcaccgtgtc
     1321 ggtgccgcga ctcgccgcac aattccaccc catgatggtt accgggccgt aacggcgtcg
     1381 gcgaagcgtg cgcggaaccc ggcgggccgt ggaagcgtct ttcgcggacg atggccgagt
     1441 tcgaccgcct tgccgtgaag cccgggctgc cgccgatgga ggcgaagctg gtcgatgccc
     1501 tgcccgaggg cgaaggatgg cagtacgagc ccaaatggga cggcttccgc tgcctggtct
     1561 tccgggacgg cccggacgtc gccctcatgt ccaagtcggg caagccgctc ggccgctact
     1621 tcccggaggt ggtcgacacg gtgctgggga tggaggccga ccgggtcgtg ctcgacggcg
     1681 agctgatcat cccggtggac ggagcgctct cgttcgacgc cctccagctg cgccttcatc
     1741 ccgccgagag ccgggtcgcc cggctgtcgc gcgagactcc cgcgcagctg atgctgttcg
     1801 acctgctctt ctcgaaggag acgagctatc tcgaggcgcc gctcgagctg aggcgccggt
     1861 cgctcgagat catctacgcc gccgagcagc atgacgacct cctgctctcc ccctgcacga
     1921 ccgagctcga ggcggcgcgc cgttggctcg aggaggtcgg cgccgagctg gacggagtcg
     1981 tcgccaagcg gatcgacgcg ccctaccagc ccggcgagcg ggcgatgctc aagatcaagg
     2041 cgatgcggac cgcggactgc gtcgtcggcg gcttccgcta tgcgacgaac agccgcgagg
     2101 tcggctcgct cctcctcggc ctctacgacg aggccgggct gctccaccat gtcggcttca
     2161 cctcctcgat cccggcggcc gagcgggcgg agctgacccg gcggctggaa gcgctggtcg
     2221 aggcgccggg cttcaccggc gactcgccgg gcggcccgag ccgctggtcg accgagcgca
     2281 ccggcgaatg gcagccgctg cgctccgagc tggtcgccga ggtgcgctac gatcacgtca
     2341 cgggccgccg cttccgccac ggcacccggt tcctgcgctg gcggcccgac aaggcgcccg
     2401 agcaatgcac gatggagcag ctccacgccg aggcgccccc cgaggcgctc gcggcccggc
     2461 tggcggcggc gtcgtgacgg ccgagggttt cgtcgccggg gtgcggctca gcaaccccga
     2521 caaggtgctc tatccggagc agggcatcac caaggggcag ctcgccgatt attacgtcgc
     2581 ggtcgccgac cggatgcttc cgcacgtcgg cttccggccg gtgacgatgg ttcgctgccc
     2641 gatggggcag gagacgaaat gcttctacca gcgccacgcc ggttcgggcg tgccggaaca
     2701 gctgcgcgag gtggcgatcc cgggcttcga cgagccttat ctgtacattt gcgacgtttc
     2761 gggactggtc gcgatggtgc agatgggcgt gctggagatc catccctggg gcgttcgcgt
     2821 cgaagccccc gaccgagccg accggatcgt cttcgacctg gatccggccg aagggctcgg
     2881 cttcgaggcg gtgatcgagg cggcgcgcga ggttcggcag cggctgaagg cgctcgggct
     2941 gacgagcttc gtcaagacca ccggcggcaa gggcctccac gtcgtcgtgc cggtcgaggc
     3001 gacggcgccg tggaaggagg tcaagagctt cgccaagggg ctctccgccg agatggcggc
     3061 cgactcgccg gacaaatatc tgaccacggc cggcaaggcc gaccgggtcg ggaagatctt
     3121 catcgactat ctgaggaacg acccgacctc gaccgccgtc gccccctatt ccacccgctc
     3181 ccgcaagggc gctccggtgg cgatgccgat ccggtgggag caggtgaagg cgggcctcga
     3241 cccgtgcgac tatacggtgg agacggtgcc ggccctgctc cgccgccagc ggagcgaccc
     3301 gtgggccgag atgctcgagg tgcggcagac gcttccggac gggccgaggg cctgatctgc
     3361 ctccccatct cgcgatgggg aggcagggtt gccgccgcgt cccgacttcc tatgtggaca
     3421 tcgaaggagt ctcacaatgg ccgagcagca ggcgatcctc gcgggcgggt gcttctggtg
     3481 caccgaggcg gtgttcaacg acgtcatcgg agtgacgcgc gtcgagagcg gctatatcgg
     3541 cggaaatgtc gccaatccca cctacaagca ggtctgcggc ggcgataccg gccatgcgga
     3601 ggcgatccgg gtcaccttcg atcccgagat catctcctat gacgagcttc tcgacctctt
     3661 cttcgccacc cacgatccga cccagctcaa ccgccagggc aacgacgtcg gcacccaata
     3721 tcgctccgcg ctgttcccgc tctccgcgga gcaggaggcg gcggcgaacc gcgcgatcga
     3781 gcgggcgcgg gcggactggc cgggaccgat cgtcaccacc atcgagccgg tcggcgactg
     3841 gtggccggcg gaggattatc accaggatta ttgggcgggc gagggccagc gcaatcccta
     3901 ttgcctcgcc gtcatcccgc ccaagctgaa gaagctgcgc aagagcttcg cgcacagggt
     3961 gaagagcgag gccgccgttt ccgaatagcg ccatccggtt attcgataat tgttgtttgg
     4021 caagggcttc tgtgccaaat tcctccccga acgggcattt ctcgcccggc atgggggatg
     4081 aagacatgaa catgatgaag tggacggcgc tcgccgtcgt cgccgccatg gcggccgctc
     4141 agccggccgt tgccaagaag gcgcgcacct atatcgtcaa caacgaggcg gtcggcgttc
     4201 ccgtcttccc gcacgacatc accgaccggc cctacacggt catcggcgag gtcaaggcgg
     4261 gcgtgcgcaa ggcgaccgtc ttcagcaagg cggcttcgca gaagaaaatc tatcgcgagc
     4321 tgtgggagcg cgcgcgcaag aagggcgccg atgcggtgat caacgccagc tacggcgatt
     4381 cgaagatcag cgcgatgagc tggggccaga ccaacgcccg cggcaccgcg atccgcttca
     4441 cgacgccgcc ggcctccaac taggcccggc gtctccgatt ccgaggaggg gaacatgccc
     4501 gtcagattga tcgcgctcgc cctgctcgcc tcggcgagcg gcgcggccgc gcaggaggcg
     4561 gcgtccgccg ccgcccagcc cgatcttgtg gtcgcgactc cggcggcgcc ggcggaccgg
     4621 cccggcgcca tcgtcgtcgc ccgggacacg atggtccggc tgatggtgct gaacgaggtc
     4681 aacaccaaca gcgccaagcc cggcgaccgc ttcgtgctca gggtggacga gaatgtcgtc
     4741 gtcggcggct cgacggtgat cccggtcggg gccaaggcct atggcgaggt cacccgggtc
     4801 aagtccaacg gcgcggtcgg caaggccggg tcggtcggcg ccaaattgct ctacgtggag
     4861 ctcggcgagg accgcgtccc gctcatcggc gagagcgaat ccaagggccg caaggaaggc
     4921 ggcggagtgg tcatggcgtc tgccatctgg ggcccgttcg ggctgctgat gaagggcggg
     4981 gcgggcaagc tcaaggccgg ccacatcttc aacggctata tcccggcgga cctgctctac
     5041 gatccggcca gcggccggtt catcgacccg gaggcgaagg cggaggcggc ggccgcgcca
     5101 tgaagcacgg ccgcgccctg ctcgcggcgg tcctgctcat cgccgcgccg gtcggcgccg
     5161 gcgctcagcc ggcccccggc ccggtgctcc tgaagggcac cccggtcgtg ttgaagaccc
     5221 tcgcgccgct gagctcgagg cgggcccgcc agggccagcg cttcgacgtc gaggtcagcg
     5281 acgagatccg ggtcgacggc ctgctcgtca tcccgagggg cgcgcgcggg gtcggcgagg
     5341 tcagccgggt cgtcaccaag gggatggtgg gcaagcccgg caagctcgag gtgcgcgtgc
     5401 tgtacgtcga ggtcgccggc aggcgcatcc ggctcgacgg cgtggccagc gacaagggcg
     5461 cgtccggcgc ggcgccggtc gtgctggcgg cgccgctgat cggggtgagc gcggccttct
     5521 tcaccggcaa gagcgccgtc cttccggccg gctcgaccat cgagggcttc gtctaccagg
     5581 acatgccgct ggtccggccg tccggcgact gatcgccgcg cgcacttttt cccgcggccc
     5641 gccgggccta tgacggatcg atgaatcggg cttccggcgc gctcctgggc ggattgctgg
     5701 ccgcggcggc ctgggcgccg gcgccggccg acgcccggac cgcccgcggc tgcgccatct
     5761 ccctcacggc cgcggccgac ggcagcttcg cccaggccgg ctacaattcg ggcccggccc
     5821 ggctggcgaa gcttgcggcg gcgacccggg agcgcttcac ggccgccgcg ctgcggctct
     5881 gcgcgctgaa ggtcctgacc cccggggatc tggcgcgctt cggccgcctc gtcgtccaga
     5941 acggggaggg cgcgaccgag ccgctcctct accaggatcc ggcgatgggg ccgcgggtct
     6001 tcatcttcca gttcgccttc caggacggcg gcccgcccga gccggccgaa ttcgagcagg
     6061 cgctgcgctg ctggaagcgg ccgaagaggt gcgacttcgg ggattgaggc gtggcgtatc
     6121 gaagcattcc agcatccgga agcataacct atcttctggg aaaatagatt tatccggcct
     6181 gccaatagcc tgctgattgt cctaaaatca taaggagttg tcgcctcgac tcgacttgcc
     6241 aagcggcgaa gagtcgtcca gcttccctcg atgagtcgta ttggggcgac tctattcggg
     6301 gggttacaat gaaaaatatt cacagggcgc tgctcgtcag cgcgtcactg acgatcgtgc
     6361 ccgtcgcggc cgccgcgcag atcgcgcccg tggctcagcc cgcggtgtcg ccgatcatcg
     6421 cccctgcgcc cacgagcgcc gtccttcgcg tcggaaccga ggtgccgttg aggctgaggg
     6481 aggagctgac caccaagggc aagaagatcc ggaccggcta ccgcttccag ctcgagacct
     6541 cggagcctgt gatggtccag ggggtcaccg tgattccgcc cggaagcccg gccgtgggag
     6601 aggtcaccag cgcccgcaac aaaggcatgt ggggcaaatc cggcaagctg aacgcccgca
     6661 tcctttatgt gatggtcaac ggacgccaga ttcgcctcac cggcaatttc gacgacaagg
     6721 ggactgccgg cggcattgcc gccgtcgcgg tgtccgccgt cgtgtttctg ccggccggct
     6781 tcttcatgac cgggacgagc gccaaggttc cagcgggaac catcgtcaag gccttcgtcg
     6841 acgaagacgt gcctctggcg ctggcggccg cgccggtcca gcccttgacg gtcgcagctc
     6901 cggccgcggt catgaccgtc gcggcgccgg tccccgcggc tgtcgcgccg gc
//
LOCUS       DASVQZ010000368         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_847808, whole genome shotgun sequence.
ACCESSION   DASVQZ010000368 DASVQZ010000000
VERSION     DASVQZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847808"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1410
                     /locus_tag="VF547_10465"
     CDS             22..1410
                     /locus_tag="VF547_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8263283.1"
                     /translation="MAAAFSLLFLAAPALAQTLAITGGTVALGDGSEPIEGGTVVIRN
                     GRIVAAGRDVAVPAGAQRFDATGKWVTPGIVAGFSRVGLLEVDAVNATNDVSANNSPF
                     SAAIDVAPAINPRAAAIQVSRAGGVTRAIVAPGTAKSIFAGQGAVIDLGNDMEAVTRA
                     RAFQFVELGETGADDAGGSRAAAHVLFRNALREARDLGRFSAPISGGGGGREPRASDQ
                     RDFPLEEVPDNRLISAGATRPDDVLLTRFDAAALVPVLQGRQLLLVHAERASDILQVL
                     ALTKEFPRLRLVLVGATEGWTVADRIAAAGVPVIANALNDLPATFEQLAATQSNVARL
                     RSAGVKVALGMINDDEARQVRLARQQAGNMVAVGRLPGASGLSWGEALATITSAPAEA
                     VGMGGEIGSLRAGRRADLVIWSGDPLENSSAAELVLIDGVQQPLDNRQTKLRDRYRNP
                     VEGDLPKAYDRR"
     gene            1457..2158
                     /locus_tag="VF547_10470"
     CDS             1457..2158
                     /locus_tag="VF547_10470"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX8263284.1"
                     /translation="MAGMTMLASLALATLAFVGTHLAMSHPLRLRLVGNLGEQWFLAL
                     YSAVALVTLGWMILAWGAVSNSVALWVAPDWAWPLGSGVMLLACILLVGSFFRNPAFP
                     HPGSKPQAVRPATGIFAVTRHPMNMAFILWALVHIGLWGSPRNLIVAGGILVLAVLGS
                     IGQDGKKLDVVGEPWRQWMARTSFVPFQALLAGRAKWRGLIEAWPAVLGGLLLWLAIT
                     WFHAPEASPVAMLTS"
     gene            complement(2301..3575)
                     /locus_tag="VF547_10475"
     CDS             complement(2301..3575)
                     /locus_tag="VF547_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8263285.1"
                     /translation="MSQATRILLALAAGLALGILASWTGGRWVDTAVLVANPVGGLWL
                     DALRMTIIPLIVSLLITGIAASAEAAQASRLAGRAILTFLAVLWTSAILAAFLTPLLL
                     SFWPIPGESAAALKSALGASTEKVGEIPTIGQFVRSIIPTNPIAAAANDHILPLMVFT
                     AIFGFALTRLPHGPREQLTGFFRAVGDVMLVMVNWVLWLAPIGVFALAFVVGATAGSG
                     AIQALLHYILIVSAVGCVVWFAAWPLAMAAGRKGFLAFTRAVGPAHAVAISTQSSLAS
                     LPAMLKGTEKLGIPVARSGVVLPLAVAIFRATGPAMNLAVAVYVAHWYGIELTPMMLV
                     AGVAAAATTTLGAVSLPGSITFITSIAPICFAMGVPIEPLVLLVAVETLPDIVRTTGN
                     VAMDVAVTTAVTRGGEEAPLTEQDRLLSEGTA"
     gene            3852..>4195
                     /locus_tag="VF547_10480"
     CDS             3852..>4195
                     /locus_tag="VF547_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX8263286.1"
                     /translation="MTRKFFGTDGIRGRTNQLPMTAAMAQRVGQAAGAHFQRGDHRHR
                     VVIGKDTRLSGYMMETALISGFTSVGMDVVMVGPVPTPAVAMLTRSMRADLGVMISAS
                     HNPYVDNGIKLFG"
BASE COUNT          619 a         1456 c         1524 g          596 t
ORIGIN      
        1 tccccgttcc ggggaggagc aatggccgcc gctttctccc tcctcttcct cgccgccccc
       61 gcgctcgccc agacgctcgc gatcaccggc ggcacggtcg cgctcggcga cggctccgag
      121 ccgatcgagg gcgggacggt cgtcatacgc aacggccgca tcgtcgcggc cgggcgcgac
      181 gtcgcggtgc cggcgggcgc gcagcgcttc gacgcgaccg gcaagtgggt gactccgggg
      241 atcgtcgccg gcttctcgcg ggtcggcctg ctcgaggtcg acgcggtcaa tgcgaccaac
      301 gacgtctcgg ccaacaattc cccgttcagc gccgcgatcg acgtcgcgcc cgcgatcaac
      361 ccgcgggcgg ccgcgatcca ggtcagccgc gccggcgggg tcacccgagc catcgtcgct
      421 ccggggacgg cgaagagcat cttcgccggc cagggcgcgg tgatcgacct cggcaacgac
      481 atggaggcgg tgacccgcgc gcgcgccttc cagttcgtcg agctgggcga gaccggcgcc
      541 gacgacgccg gcggcagccg cgccgcggcc cacgtcctgt tccgcaacgc gcttcgcgag
      601 gcgcgcgacc tcggccgatt ctcggctccg atctcgggcg gcggtggcgg ccgcgagccg
      661 cgggcgagcg accagcgcga cttcccgctc gaggaggtgc ccgacaaccg actgatctcc
      721 gccggggcga cccggcccga cgacgtgctg ctcacccgct tcgacgcggc cgccctggtg
      781 ccggtgctgc aggggcggca attgctcctc gtccacgccg agcgggcgag cgacatcctc
      841 caggtgcttg cgctcaccaa ggagtttccg cgtctcaggc tggtcctggt cggagcgacc
      901 gaaggctgga ccgtcgccga ccggatcgcc gcggccggag tgccggtgat cgccaacgcg
      961 ctcaacgacc tgcccgcgac gttcgagcag ctcgccgcga cccagtccaa cgtcgcccgg
     1021 ctgcgctcgg caggcgtcaa ggtcgcgctc gggatgatca acgacgacga ggcccggcag
     1081 gtgcggctcg cccgccagca ggccggcaac atggtcgccg tgggccggct gcccggcgcc
     1141 tccggcctca gctggggcga ggcgctggcg acgatcacct ccgcgccggc ggaagcggtc
     1201 ggaatgggcg gcgagatcgg ctcgctgcgg gccggccggc gcgccgacct cgtcatctgg
     1261 tcgggcgacc cgctcgagaa cagcagcgcc gccgagctgg tcctgatcga cggggtccag
     1321 cagccgctcg acaaccgcca gaccaagctt cgcgaccgct accgcaaccc ggtcgagggc
     1381 gacctgccca aggcctacga tcggcgatga ccaggcccgg attgcgcccg gcaatccgta
     1441 gccgtgtccc cggggcatgg cgggcatgac catgctggcc agcctcgcgc tcgcgaccct
     1501 ggccttcgtc ggcacccacc tggcgatgtc gcaccccctg cggctgcggc tggtcgggaa
     1561 cctcggcgag caatggttcc tggcgcttta ttccgcggtg gcgttggtca cgctcggctg
     1621 gatgatcctc gcctgggggg cggtgtcgaa cagcgttgcg ctctgggtcg cgccggactg
     1681 ggcctggccg ctgggctccg gcgtcatgct gctcgcctgc atcctgctgg tcggctcgtt
     1741 cttccgcaat ccggcctttc cccatcccgg gtcgaagccg caggcggttc ggccggcgac
     1801 cggcatcttc gccgtgaccc gccatccgat gaacatggcc ttcatcctgt gggcgctggt
     1861 ccacatcggc ttgtggggaa gcccccgcaa cctgatcgtc gccggcggca tcctcgttct
     1921 ggccgtgctc ggctcgatcg gccaggacgg caagaagctc gacgtggtcg gcgagccgtg
     1981 gcgccagtgg atggcgcgca cctcgttcgt cccgttccaa gccctgcttg ccggacgggc
     2041 gaaatggcgc gggctgatcg aggcctggcc ggccgtgctc ggcgggctcc tcctgtggct
     2101 cgccatcacc tggttccacg cgcccgaagc ctccccggtc gcgatgctga cgagctaagc
     2161 accgtgggcc tcaattcctc cccgcgattt cgcggggagg gggaccagcc gtaggctggt
     2221 ggaggggttc tcgatgagtc cgaagatccc ctccaccatg cttcgcatgg tccccctccc
     2281 cggccaaggc tggggaggaa ttaggccgtc ccctcgctca gcagccggtc ctgctcggtg
     2341 agcggcgcct cctcgccgcc gcgggtgacg gcggtggtga cggcgacgtc catggcgacg
     2401 ttgccggtgg ttcgaacgat gtcgggcagg gtctcgaccg cgacgagcag gacgagcggc
     2461 tcgatcggca cgcccatggc gaagcagatc ggcgcgatcg aggtgatgaa ggtgatcgag
     2521 cccggcaggc tgaccgcgcc gagcgtggtg gtcgcggcgg cggccacgcc ggcgaccagc
     2581 atcatcgggg tgagctcgat tccgtaccag tgggcgacgt agaccgcgac ggcgaggttc
     2641 atcgccggtc cggtcgcccg gaagatggcg acggcgagcg gcagcacgac gccggagcgc
     2701 gccacgggga tgccgagctt ctcggtgccc ttcagcatcg ccggcagcga ggcgagcgag
     2761 gactgggtgc tgatcgccac cgcatgggcg ggcccgacgg cgcgggtgaa ggcgagaaag
     2821 cccttgcgcc ccgccgccat ggccagcggc caggccgcga accagacgac gcagccgacc
     2881 gccgagacga tcagtatgta gtggagcagg gcctggatcg cgcccgagcc cgccgtggcg
     2941 ccgacgacga aggccagcgc gaagactccg atcggcgcca gccagagcac ccagttgacc
     3001 atcaccagca tgacgtcgcc gacggcgcgg aagaagccgg tgagctgctc gcgcggaccg
     3061 tgcgggaggc gggtgagggc gaagccgaag atggcggtga acaccatcag cggcaggata
     3121 tggtcgttgg cggcggcggc gatggggttg gtggggatga tcgagcggac gaactggccg
     3181 atggtcggga tctcgccgac cttttcggtc gaggcgccga gcgccgactt cagcgccgcc
     3241 gccgattcgc ccgggatcgg ccagaagctg agcagcaacg gggtcaggaa ggcggcgagg
     3301 atcgcgctcg tccagaggac ggcgaggaag gtgaggatcg cccggccggc gagccggctc
     3361 gcctgggccg cctcggccga ggcggcgatg ccggtgatca gcagcgacac gatcagcggg
     3421 atgatcgtca tcctgagcgc gtcgagccac aggccgccga ccgggttggc aaccagcacg
     3481 gccgtgtcca cccagcgccc gccggtccag gacgcgagga tgccgagcgc gagcccggcg
     3541 gcgagggcga ggaggatgcg ggtggcctgc gacatccggg ggttctagcg gcggagcggg
     3601 gaccgtggga agggggaccg gatgaaggcc ggtggagggg tccttcgata cgggccttcg
     3661 ccgggctcag tccctactca ggatgagcgg ctggatatct ctgccgctca tcctgagtag
     3721 ccgctgagcg aagtcgaagc ggcgtatcga aggacccctc caccaggctt cgcctggtcc
     3781 ccctgcccat ctgcgatggg gaggacaagc agccgccttc gcgctattca gcgccaaaac
     3841 aagggagctc catgactcgc aaattcttcg gcaccgacgg gatccgcggc cgcaccaacc
     3901 agctgccgat gaccgccgcc atggcgcaac gggtcggcca ggcggccgga gcgcatttcc
     3961 agcgcggcga ccatcgccac cgggtggtga tcggcaagga cacccgactg tccggctata
     4021 tgatggagac ggcgctcatc tcgggcttca ccagcgtcgg aatggacgtg gtgatggtcg
     4081 ggccggtgcc gactccggcg gtggcgatgc tgacccgttc gatgcgcgcc gatctggggg
     4141 tgatgatctc cgccagccac aacccctatg tcgacaacgg catcaaattg ttcgg
//
LOCUS       DASVQZ010000369         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_754221, whole genome shotgun sequence.
ACCESSION   DASVQZ010000369 DASVQZ010000000
VERSION     DASVQZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754221"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..490)
                     /gene="sdhD"
                     /locus_tag="VF547_10485"
     CDS             complement(59..490)
                     /gene="sdhD"
                     /locus_tag="VF547_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX8263287.1"
                     /translation="MTEGTGETGASPATRPAAETPLARVRGLGSAREGAEHWWHERLS
                     SVAVLLLFVWLLVSLLRLPALDHRGVTEWLRDPRSAVPMLLLVGATFWHLQMGLRVVI
                     EDYVHDSGNRFLALLLLSFAAILGAAFAALAVLRLALGAPA"
     gene            complement(487..888)
                     /gene="sdhC"
                     /locus_tag="VF547_10490"
     CDS             complement(487..888)
                     /gene="sdhC"
                     /locus_tag="VF547_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX8263288.1"
                     /translation="MSRNPARPLSPHLGVYKWGPHMLVSILHRITGVGLALVGAAALV
                     WWLVAAASGEEAYGDFAGWATSWLGILVGIGLTWAFFQHLLSGLRHLVMDIGAGFEIE
                     RNKFWAMMTLAGSLLLTAALWLYIVLVRNAA"
     gene            complement(913..1350)
                     /locus_tag="VF547_10495"
     CDS             complement(913..1350)
                     /locus_tag="VF547_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8263289.1"
                     /translation="MTPAVEVAGLSKRFGRGERAVPALDGLSLAIPAGGIFGLLGANG
                     AGKSTLMRIVAGPVLPAFLLSLAAATLEALLDLRGDALAMLPLPTLAADAVRAALFAW
                     SAAAYAAAFTWFRRQDLAAERDRCCLAGAGPLGASPCIPPPTP"
     gene            complement(1369..1845)
                     /locus_tag="VF547_10500"
     CDS             complement(1369..1845)
                     /locus_tag="VF547_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8263290.1"
                     /translation="MMAAGRDEAMFLKGGKAPAPGQGGGAFSYLGSEVTVTGDIVTES
                     ELHLDGKVTGDVRCGALTQGESGEVRGNIKAGEARLAGLVDGAVEAAALTLEASARVT
                     GDILYETLSVAAGAEVEGRFKRRRGAADGSAVARTEKPPPAPAPLFGTEPSAQAAE"
     gene            complement(2885..3262)
                     /locus_tag="VF547_10505"
     CDS             complement(2885..3262)
                     /locus_tag="VF547_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263291.1"
                     /translation="MAKGDGKKGRKDKLKVPKRIAGVKVSKAVRKAGKTALKLAEQPA
                     VSETVAAALLAAAAALRDPPAAKRGARAAADAAGEAGQEAIRLGDSLRRLAIDVARRT
                     LEAWEEAESKPRGGAEGDGGGNG"
     gene            complement(3275..3658)
                     /locus_tag="VF547_10510"
     CDS             complement(3275..3658)
                     /locus_tag="VF547_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263292.1"
                     /translation="MRSIVLLLALLLAAPAAAQDWRMAPEYDVQLSTYAIQPGEIRLR
                     AGEPVRLRFVNNSNQQLTFSAGAFFRSAQMRRRDARLVRGGAVRVPPLSTRTVVLVPR
                     PGRYRAQGANLLHRLLGMSGRIIVE"
     gene            3867..5165
                     /locus_tag="VF547_10515"
     CDS             3867..5165
                     /locus_tag="VF547_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8263293.1"
                     /translation="MRFLILALLLLAAPAAAKLAPAERRIVGAVEAEQPRAVALLERL
                     VNQNSGSLNLRGVEAVGRMMRAELEPLGFTVRWVPMAEVARAGHIVATHRGSGRGKRM
                     LLIGHLDTVFEPDSPFQSFRLKDPQTAEGPGVGDDKGGMVVMVAALRAMQAAGTLKDA
                     DIEIVLTGDEEDAGAPIGIARRDLIAAAGRADVALDFEGLAREAGRDMGSIARRGSAS
                     WTVTAHGRTGHSSGIFGPSMGNGAIYELARILAAFRSELPEPNLTFNVGLVAGGATAE
                     LDKDGIRATATGKTNIVPPIAIARGDLRALSLEQQERVIAKMREIVGRHLAGSSAEII
                     FDDGAYPPMAPTEASRALLDRLNAVNRDLGLAEMGELDPLKRGAGDIGFVAHLLPGLV
                     GLGTAGDGAHAAGETADLASIPRQAKRAAILMSRLARERR"
BASE COUNT          787 a         1972 c         1938 g          739 t
ORIGIN      
        1 atagaaggca cgagctgctg ctggcgccga agcggccgga gccgcggatc cggccgctct
       61 aggcgggggc gccgagcgcg agcctgagca ccgcgagcgc ggcgaaggcg gcgccgagga
      121 tggcggcgaa ggagagcaga agcagcgcga ggaagcggtt gcccgagtcg tggacataat
      181 cctcgatcac cacccggagg cccatctgca ggtgccagaa ggtcgccccg acgagcagca
      241 gcatcggcac cgccgagcgc gggtcgcgaa gccattcggt cactccgcga tggtcgagcg
      301 cgggcagccg caggagcgag acgagcagcc agacgaacag caggaggacc gccaccgacg
      361 acagccgctc gtgccaccaa tgctcggccc cctcgcgcgc cgagccgagg ccccggaccc
      421 gcgccagcgg ggtctcggcc gccggccggg tcgccgggct cgctcccgtc tcgccggtcc
      481 cctcggtcat gccgcgttcc tcaccaggac gatgtagagc cacagggcgg cggtgagcag
      541 cagcgatccg gccagagtca tcatcgccca gaatttgttg cgctcgatct cgaagccggc
      601 gccgatgtcc atgaccaggt gcctgaggcc cgagagcaga tgctggaaga aggcccaggt
      661 gaggccgatc ccgaccagga tcccgagcca ggacgtcgcc cagccggcga agtccccata
      721 ggcctcctcg cccgaggcgg cggcgaccag ccaccagacc agggcggcgg cgccgaccag
      781 ggcgagcccc actccggtga tccggtggag gatcgagacc agcatgtgcg gcccccattt
      841 gtagacgccg agatgcggcg agagcgggcg ggcgggattg cgggacatcg aggctcctct
      901 cggctcgcgc ttttagggcg tcggcggggg gatgcaaggg gacgcgccga gggggccggc
      961 gcctgcgagg cagcaacggt ctcgctcggc cgccaggtcc tggcggcgga accaggtgaa
     1021 ggcggcggcg taggcggcgg cggaccaggc gaagagcgcc gcgcgcaccg cgtcggcggc
     1081 cagggtcggc agcggcagca tcgccaaagc gtcgccgcgc agatcgagca gcgcctcaag
     1141 cgtcgccgcc gcgagcgaga gcaggaaggc gggaaggacc ggccccgcga cgatccgcat
     1201 cagcgtgctc ttgcccgcgc cgttggcgcc gagcaggccg aagatgccgc cggcggggat
     1261 ggcgagggac agcccgtcca gcgccggcac ggcgcgctcg ccgcggccga agcgcttgga
     1321 gaggcccgcg acttcgacgg cgggggtcaa gtcgggatcg ccggcgtcct attccgccgc
     1381 ctgggcggag ggctcggtgc cgaacagggg cgcgggcgcc ggcgggggct tttcggtcct
     1441 ggcgacggcc gagccgtcgg cggcgccgcg gcggcgcttg aagcggccct cgacctcggc
     1501 gccggcggcg acggagaggg tctcgtagag gatgtcgccg gtgacccggg cgctggcctc
     1561 gagggtgagg gcggcggcct cgaccgcgcc gtcgaccagg ccggcgagcc gcgcctcgcc
     1621 ggccttgatg ttgccgcgaa cctcgccgct ctcgccctgg gtcagggcgc cgcagcggac
     1681 gtcgccggtc accttgccgt cgagatggag ctcgctttcg gtgacgatgt cgccggtgac
     1741 cgtgacctcg cttcccagat aggagaaagc gccgccgccc tggcccggcg ccggcgcctt
     1801 gccgcccttc aggaacatcg cctcgtccct ccccgcggcc atcatgacac aatgaggagg
     1861 cggatcaaga gaccgaattc gcggctcggt gcgaacccct ttttcaaggg aaggcacgaa
     1921 ggcacgaagc ggcccccggc gcgaagcgtc ctcagtcctg gaaacaggca catatgaatt
     1981 ggggcgcgag gccagcttcg cgccttcgtg ccttcccaca aaagcgggcc tgggcggggc
     2041 aggagaagcg cgagatgcgc cggcccggac aatggaggcc ggaaacgata atgggggccg
     2101 gtgtcgccac cggcccccac ttgtcccagc cttgactgcc gtcgctgggt ggcgcggcca
     2161 tctcgcgctg ggcggcttcg ggagcgcgcc ggggcgcgag gccccggccg cttccctgga
     2221 tcccggagcg agagctcacg ctcccgttcg ggccccggtg ggtctcggag agaggcccgg
     2281 aagcccctcg ccaatccccg gcgggtcgcg gcaagcgggg ccgaagccct gccagccgcc
     2341 tccctttggc ggatccggcc gggccgaagc ctggccgtgt ccttcggctc gcatcgaggc
     2401 cgaagcctct ccggtcgccg cttcgaaagc gctgggccga ggccttcccg ctttccggga
     2461 gcctcgggag gccgaagccc ccgaaacccc cgttcgggtc gcggccgggg ccgaagcccc
     2521 tgccgtctcc ctgccgtctc cggccgcggg gcgattcccg cggccttcgc cggttcggcc
     2581 ggtcatcgag gctccgtaag gcgcccctcg gcccgaccct ggaggcttcg tgaaatcccg
     2641 ccgtgcatcc gcttgcgctc atgccggcgc ggcttcgtcc gcctccccgc cttccgcttc
     2701 ggcgccagcg tccttggggg agcgacggcg ccttcggctt cgggcatcgc ggccctgggt
     2761 gcggcgttcc gtttccggtc cgccgcgctt tccggccacg agctcaagct gtcagcttta
     2821 cccgattcgg ggaagggtcc ggacgctgtg gataacgagg ataacggggg taagttaacc
     2881 tcgatcagcc gttgccgccg ccgtcgccct cggcgccgcc gcgcggcttc gactcggcct
     2941 cttcccaggc ctccagcgtg cgccgggcga cgtcgatcgc cagccttcga agcgaatcgc
     3001 cgagccggat cgcctcctgc cccgcctcgc cggccgcgtc cgccgccgcc cgggcgccgc
     3061 gcttggcggc cggcggatcg cgaagcgctg cggccgcggc cagcagcgcc gccgccaccg
     3121 tctcgctgac cgccggctgc tcggcgagct tcagggccgt cttgccggcc ttgcgcaccg
     3181 ccttggacac cttgaccccg gcgatccgct tgggaacctt gagcttgtcc ttcctgccct
     3241 tcttgccgtc gcccttggcc atgtctcgcg ctccctattc gacgatgatc cggccgctca
     3301 tgccgagcag ccggtggaga agattggcgc cctgcgcccg atagcgcccg ggcctcggca
     3361 ccagcacgac ggtgcgggtc gacagcggcg gcacccgcac cgctccgccc ctcaccagcc
     3421 gggcgtcgcg ccgccgcatc tgcgcggacc ggaagaacgc gccggccgag aaggtcagct
     3481 gctggttgct gttgttgacg aagcgcagcc gcaccggctc ccccgccctc agccggatct
     3541 cgccgggctg gatcgcgtag gtcgagagct gcacgtcata ttccggcgcc atccgccaat
     3601 cctgggcggc ggccggagcg gcgaggagga gggcgaggag cagaacaatg gatcgcatat
     3661 ggaaaaccta gcacggcggc ctatccttgt cctccccgcc gcgaagcggc ggggagggga
     3721 ccgcacgaag tgtggtggag gggtaattgg cgcaggcggg cctggttccc gcgaattcct
     3781 cttgcccaga gccagttacc cctccaccag ccttcggctg gtccctctcc ccatcttgcg
     3841 atggggagga tgaatggagc ctcaccatgc gctttctcat cctcgccctt cttctcctgg
     3901 ccgccccagc cgcggcgaag ctcgcccccg cggagcggag gatcgtcggc gcggtggagg
     3961 ccgagcagcc gcgcgccgtc gccctgctcg agcggctggt gaaccagaac agcgggtcgc
     4021 tgaacctgcg cggggtcgag gcggtcgggc ggatgatgcg cgccgagctc gagcctctcg
     4081 gctttacggt gcgctgggtg ccgatggccg aggtcgcccg cgccggccac atcgtcgcca
     4141 cccatcgcgg cagcggccgc ggcaagcgga tgctgctgat cggccatctc gacacggtgt
     4201 tcgagcccga cagcccgttc cagagcttcc ggctcaagga cccgcagacc gccgaggggc
     4261 ccggggtcgg ggacgacaag gggggcatgg tggtgatggt cgccgcgctt cgagcgatgc
     4321 aggcggcggg aaccctgaag gacgccgaca tcgagatcgt cctgaccggc gacgaggagg
     4381 atgccggcgc tccgatcggg atcgcccggc gcgacctgat cgccgccgcc ggccgagccg
     4441 acgtcgcgct cgacttcgag ggcctggccc gcgaggcggg ccgcgacatg ggctcgatcg
     4501 cccggcgcgg ctcggcctcc tggacggtga ccgcgcacgg ccggaccggt cattcgagcg
     4561 gcatcttcgg gccgagcatg ggcaatggcg cgatctacga gctcgcccgc atcctcgccg
     4621 ccttccgctc cgagcttccc gagcccaacc tcaccttcaa cgtcggcctc gtcgccggcg
     4681 gcgccaccgc cgagctcgac aaggacggga tccgcgcgac cgccaccggc aagaccaaca
     4741 tcgtcccgcc gatcgccatc gcccgcggcg accttcgcgc gctgtcgctc gagcagcagg
     4801 agcgggtgat cgccaagatg cgcgagatcg tcggccgcca cctcgcgggg agcagcgccg
     4861 agatcatttt cgacgacggc gcctatccgc cgatggcgcc gaccgaggcg agccgggcgc
     4921 tgctcgaccg cctcaacgcc gtcaaccgcg acctcggcct cgccgagatg ggcgagctcg
     4981 atccgctgaa gcgcggcgcc ggcgacatcg gcttcgtcgc ccatctcctg cccggcctgg
     5041 tcggcctcgg caccgccgga gacggcgccc acgccgcggg cgaaaccgcc gatctcgcct
     5101 cgatcccccg ccaggccaag cgcgccgcca tcttgatgag ccggctcgcg agggagcggc
     5161 gctagagcgc ccggcagtcg ggtcgcgctc tcttccgttc gggctgagct tgtcgaagcc
     5221 gtactttctc tcatggcacg acaagcaaag cccttcgaca ggctcagggc gaacgggtgg
     5281 gagcgtttca accagagcgt gatcgccttc cgccgaaccg ctcatcctga gtagggactg
     5341 agcgaaggcg aaggcccgta tcgaaggacc gtccttcgat acgccggttc gacttcgccc
     5401 accggctact caggatgagc gacctaggat cactgg
//
LOCUS       DASVQZ010000370         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_369261, whole genome shotgun sequence.
ACCESSION   DASVQZ010000370 DASVQZ010000000
VERSION     DASVQZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369261"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..676)
                     /locus_tag="VF547_10520"
     CDS             complement(23..676)
                     /locus_tag="VF547_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263294.1"
                     /translation="MLKHLMLAGALGLLASPACAHSDTDESGHGSRPGWDRNDGVHRG
                     GDWGRRHDGGRRHGDWDRRDGEGGHRGGDWDRRDGDRRDGDWNRGDGDRRHRGGDWDR
                     RDGDRRDGDWDRRDDDGRHRHGDWERRDGDGRHRHGDWERRDDDRRHRGGDWDRRGHD
                     RLWWGRIPPGHLPPPGSCRVWFSARPAGRQPPPTSCSAAQSYAARHGGRVIWGARRR"
     gene            complement(733..1215)
                     /locus_tag="VF547_10525"
     CDS             complement(733..1215)
                     /locus_tag="VF547_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263295.1"
                     /translation="MALGAAAPATVASADRSLGYFAGGWDIVATTPGTGETARFAYDV
                     RPLLGTAWLSGHGRSVPPGEESKDVWGRDSASGELMRMIFDKSGTYAVVRSTGWQGDK
                     LVLEGDARSVGGVVRVRETITRLGDDAFVATWEAYRNGAWTAYSVERATRRAGSRASS
                     "
     gene            complement(1763..2086)
                     /locus_tag="VF547_10530"
     CDS             complement(1763..2086)
                     /locus_tag="VF547_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEX8263296.1"
                     /translation="MPPFKDSFQDRVGQAAEAKRKALDRLRSRPPVDEAVAETRREAA
                     RQREAKADAKRAAKAEKTRAEAAARAASKAEAATEAAAAKPPPTEAERKAARDARYAA
                     RKSRK"
     gene            complement(2086..>3359)
                     /locus_tag="VF547_10535"
     CDS             complement(2086..>3359)
                     /locus_tag="VF547_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX8263297.1"
                     /translation="LFQKIVDHVPAPGLDPEGEFKMLATLLDRDPFLGRILTGRVESG
                     RLEVNMPISAIDVDGNPVETGRATKVFAYRGLERVPVETAQAGDIVAIAGLTKATVSN
                     TIAEMAVTRAIAARPIDPPTLAMSFAVNDSPYAGRDGDKVQSRVIRDRLEREAETNVA
                     IRLTESAEKDAFEVAGRGELQLGVLIETMRREGFELSISRPRVLFRDGPNGREEPYET
                     VVIDVDDEHAGTVVEKMAMRKGEMTDMRPSGGGKTRLTFSAPSRGLIGYHGEFLSDTR
                     GTGIMNRLFERYGPYKGPISGRQNGVLISMEQGEAVAYALNALEERGILFISPGEKLY
                     EGMIIGENAKPQDLEVNPLKSKQLTNFRASGPKDESIRLTPPRRMTLEQAIAYIQEDE
                     LVEVTPRAIRLRKRFLDPHERKRQSRKAEAA"
BASE COUNT          440 a         1224 c         1145 g          550 t
ORIGIN      
        1 tccggccacg gccggatccg cctcaacggc ggcgggcgcc ccagatcacc cgcccgccgt
       61 gacgggccgc atagctctgg gcggcactgc agctcgtcgg cggcggctgg cggccggccg
      121 ggcgggcgct gaaccagacc cggcagctgc cgggcggcgg caggtggcca ggcggaatgc
      181 ggccccacca aaggcggtca tggccgcgcc ggtcccagtc gccgccgcgg tggcggcggt
      241 cgtcgtcgcg gcgctcccag tcgccgtggc gatgccggcc atcgccgtcg cggcgctccc
      301 agtcgccgtg gcgatgccgg ccgtcgtcgt cccggcggtc ccagtccccg tcccggcggt
      361 cgccgtcgcg gcgatcccag tcgccgcccc gatgccggcg gtcgccgtcg ccgcgattcc
      421 agtccccgtc ccggcggtcg ccgtcgcggc ggtcccagtc gccgccccga tgcccgccct
      481 cgccgtcgcg gcgatcccag tccccgtggc gacggccgcc gtcgtgccgg cggccccagt
      541 ctccgccccg gtgcacgccg tcgttgcggt cccagccggg gcgggagccg tggccgcttt
      601 cgtccgtgtc ggagtgcgca caggccggcg aggcgagcag cccgagagcg ccggcaagca
      661 tgaggtgctt cagcatgaag tttctccgcg gcgtcaatgt tcgaacggcg ggatcgttgc
      721 cgaggccggt cgtcaggacg aagccctcga ccccgcccgc cgtgtcgcgc gctcgaccga
      781 ataggcggtc caggccccgt tccgataggc ctcccaggtg gcgacgaagg cgtcgtcgcc
      841 caggcgggtg atggtctcgc ggacccgcac cacgccgccc accgatcgcg cgtcgccctc
      901 cagcacgagc ttgtcgccct gccagccggt cgacctgacg accgcatagg tgccgctctt
      961 gtcgaagatc atccgcatca gctcgcccga ggccgaatcc cgcccccaga cgtccttcga
     1021 ctcctcgccc ggcggcaccg accggccgtg gcccgacagc caggcggtgc cgaggagcgg
     1081 ccggacgtca taggcgaacc gggcggtctc gccggtcccc ggcgtcgtcg cgacgatgtc
     1141 ccagccgccc gcgaaatagc ccaggctccg gtccgcgctc gccacggtcg ccggcgcggc
     1201 cgcacctaac gccacgcccg acaaggccgc gaacgccatt cgaatcgact tccggggctt
     1261 tctggtcacc tcgcccgcct ccgccggaaa cagctccccc gaatgcaagg gaaaagagtg
     1321 acagtataca tttacggcac ccccaggagc ctgggaccag cgagtaaata tatactgtca
     1381 ctaattacac ccgcgcctgc cttccgggac aatccggagc cggcgctgac gatctggtac
     1441 gaaagggcct gatccggctg atccacgcgc gcgaatcgct gatgattccg atgcgccccc
     1501 gcctcaggat ggatgcccat gagatcctca agtcccggcg ctgaccggcg ccgccatgcg
     1561 atgagtacgg ctcaaccgct catcccgagt agggactgag cgaaggcgaa ggcccgcatc
     1621 gaaggaccgt ccttcgatac gcgggcttct ccggatcagg tcctgggtca ccggctactc
     1681 aggatgagca gttcgggctg ctttcggcag tcttgacccc ctttgcccac cggctgagcg
     1741 gacgccggcg tcggaagccg cttcacttgc ggcttttccg cgccgcatag cgggcgtcgc
     1801 gggcagcctt gcgctcggcc tcagtgggcg ggggcttcgc ggcagctgct tccgtcgcgg
     1861 cctcggcctt gctggcagcg cgggcggccg cctcggcccg agtcttttca gccttggcgg
     1921 cgcgcttcgc atccgctttg gcctcgcgct gccgtgcggc ttcccgccgc gtctcggcga
     1981 cggcttcatc gactggaggc ctggagcgca gcctgtcgag cgccttgcgc ttggcttcgg
     2041 cagcttggcc gacccggtcc tggaaggaat ccttgaaggg cggcatcagg cggcctcggc
     2101 cttgcggctc tggcgcttgc gctcgtgcgg gtcgaggaag cgcttcctca gccttatcgc
     2161 ccggggagtc acctcgacca attcgtcctc ctggatgtag gcgatcgcct gctccagggt
     2221 catccgcctc ggcggggtga ggcggatgct ctcgtccttc gggccggagg cgcggaagtt
     2281 ggtcagctgc ttggacttga gcggattgac ctcgaggtcc tgcggcttgg cgttctcgcc
     2341 gatgatcatg ccctcgtaga gcttctcgcc gggcgagatg aacaggatcc cgcgctcctc
     2401 gagcgcgttc agcgcatagg cgacggcctc gccctgctcc atcgagatga gaacgccgtt
     2461 ctgccttccg ctgatcgggc ccttgtaggg gccgtatctc tcgaacaatc ggttcatgat
     2521 gccggtgccg cgcgtgtccg agaggaactc gccgtgatag ccgatcaggc cgcggctcgg
     2581 cgccgagaag gtcagccggg tcttgccgcc gcccgacgga cgcatgtcgg tcatctcgcc
     2641 cttgcgcatc gccatcttct cgacgacggt gccggcatgc tcgtcgtcga cgtcgatgac
     2701 gaccgtctca tagggctcct cgcggccgtt cggcccgtcg cggaacagga cccgcgggcg
     2761 gctgatcgaa agctcgaagc cctcgcgccg catcgtctcg atcagcacgc cgagctggag
     2821 ctcgccgcgt ccggccacct cgaacgcgtc cttctcggcg ctctcggtga ggcggatggc
     2881 gacgttcgtc tcggcctcgc gctccagccg gtccctgatc acccggctct gcaccttgtc
     2941 gccgtcgcgg ccggcatagg ggctgtcgtt gaccgcgaag ctcatcgcca gcgtcggcgg
     3001 gtcgatcggc cgggcggcga tggcgcgggt cacggccatc tcggcgatgg tgttggagac
     3061 ggtcgccttc gtcagcccgg cgatggcgac gatgtcgccc gcctgcgccg tctcgaccgg
     3121 gacccgctcg aggccgcgat aggcgaagac cttggtcgcc cggccggtct cgaccgggtt
     3181 gccgtcgacg tcgatcgcgc tgatcggcat gttgacctcg agccggccgc tctcgacccg
     3241 gccggtgagg atccggccga ggaagggatc gcgatcgagc agggtcgcga gcatcttgaa
     3301 ctcgccttcg ggatcgaggc cgggcgcggg cacgtgatcg acgatcttct ggaagagcg
//
LOCUS       DASVQZ010000371         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_559291, whole genome shotgun sequence.
ACCESSION   DASVQZ010000371 DASVQZ010000000
VERSION     DASVQZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559291"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VF547_10540"
     CDS             <1..964
                     /locus_tag="VF547_10540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8263298.1"
                     /translation="HSTGRSAAFWAESYGGPFIRPLTSASGPFLQEQGLLGPRGALHL
                     ADEEGLAALAALEAEFGGEVGFERIDRQRLEALVPGLRPDWVAGLLEPSCADIDVAGL
                     HALYLASARRQGAEVSTGAEVRALRRESGAWTLETAAGSYRAAIVVDAAGAWADRVAV
                     LAGAEPIGLQPYRRTLMQLRVAPAAPASLPLVIDALGRFYFKPEAGGRLWLSPHDETP
                     CEPCDCAPEEIDVAEAIDRLERTVDWKVERVERSWAGLRTFAPDRRPVYGFDPRVPGF
                     FWCAGQGGFGIQTAPAGAKLAAALLLGRAPEIDAEPYRPGRIAR"
     gene            1276..1968
                     /locus_tag="VF547_10545"
     CDS             1276..1968
                     /locus_tag="VF547_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263299.1"
                     /translation="MGLTGCGFAGGATEVRYKVTVEVLENGSVRSGSSVWSWRLSEPT
                     VALASPYDGKLRGEAVAVELGNGRTLFAVLRGTDGESGMAEMLPERLFGDVGRSARGE
                     RTRFSPDRIADLRDIASRVGETATVPCAERPGWCPMLVTFRNISDPTSVEQVDPTDIA
                     ATLGPGYALKAITVELVSQRPEHAIEARLPWLAAVGRERASLIPNPPRLLKDTKPIQL
                     VAPSDFSTELYR"
     gene            2058..>3662
                     /locus_tag="VF547_10550"
     CDS             2058..>3662
                     /locus_tag="VF547_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263300.1"
                     /translation="MADIVTRNSLGLSDGTYNAWQEKGFYASAYDVDGAGIDGLVGTV
                     ISYRGTNFNFSWLNFFNSPLWQDIRSGWSVGAGVAGAQATMALDFYHAVKGDGVDPRD
                     AEITTTGHSLGGGLAGLAANLYGREAILFDHMTYGPISSTITRQAESSQQVRDRYFEG
                     QYPWELDPSGISAFFTEGEILRLERALFGETSSPLPSHSSGQSAVTLHSMALLTSLIW
                     AEQNADPRWKTAGALLWNSFFDEALGTSQALPGVESRAGPGGTGPAVMNSAIAYSALD
                     AGERPFGDTAIWSMFDDAAQLGSVLGSTGGPDFLGKIVDTKFSFGLGGLDIDRVTVRQ
                     HLANLLVQYAGALALYDVEQEQGGTVPTSRGPVDAREGILSRETIQSALAVDLSRTLW
                     GDVLKSNPNAVTKLPGDRLEPLDRDAFRQTYFKQAEEQGILAGVLRFLGVKSSKWDDD
                     DLREITRQLWGTDDLSILDRFHMAPVNQPTHVILDNRGYVSDTSEGSDAQVDVYIGTT
                     ANETIDNRTTGHNLIITGEGADDADGG"
BASE COUNT          567 a         1270 c         1271 g          554 t
ORIGIN      
        1 ccattcgacc gggcgctcgg ccgccttctg ggccgagagc tatggcgggc cgttcatccg
       61 gcctttgacc agcgcctcgg gacccttcct gcaggagcag ggcctgctcg gcccgcgcgg
      121 cgccctccat ctcgccgacg aggagggcct ggcggcgctg gccgcgctgg aagcggagtt
      181 cggcggagag gtgggattcg agcggatcga ccgccagcgg ctcgaggcgc tcgtccccgg
      241 gcttcggccg gactgggtcg cgggcctgct cgagccgagc tgcgccgaca tcgacgtggc
      301 cgggctgcac gccctctacc tcgcgtcggc gaggcggcag ggggcggagg tttcgaccgg
      361 cgccgaggtc cgcgcgctgc gccgcgagag cggtgcctgg accctcgaga cggcagcggg
      421 cagctaccgc gccgcgatcg tcgtcgatgc ggcgggcgcc tgggccgatc gggtcgccgt
      481 cctggccggg gccgagccga tcggcctcca gccctatcgg cgaacgctga tgcagttgcg
      541 ggtcgctccg gcggcgcccg ccagcctccc cctcgtcatc gacgcgctcg gccgtttcta
      601 tttcaagccc gaggccggcg gtcggctgtg gctgagcccg cacgacgaga cgccgtgcga
      661 gccctgcgac tgcgcgcccg aggagatcga cgtggccgag gccatcgatc ggctcgagcg
      721 gacggtcgac tggaaggtgg agcgggtcga gcgcagctgg gccggtctca ggaccttcgc
      781 gcccgaccgg cggcccgtct acggcttcga tccgcgggtg ccgggcttct tctggtgcgc
      841 ggggcagggc ggcttcggca tccagaccgc cccggccggg gcgaagctcg cggcggccct
      901 gctgctcggt cgagcgcccg agatcgatgc cgagccctac cggccgggcc gcatcgcgcg
      961 ctagcctgat ccggaatccc aaaacgcgac ggacaaaacg aaaaagggaa ggcggccgga
     1021 tatttatgtt tccgatcccg ttgctttcgc gggacggccc agcctagtgt gagttgaggg
     1081 gggattcggg gccatggggg gcactagggg cattggcgcg ccgccgcgcc gggcggacga
     1141 ccgcctcgcg ggcgcggcgc cggaagcccc gggcctgcgc ggaatcggcc ggccgccccc
     1201 gctccggcaa gcccgggacc ggagccacgg ccggtaatgg cgcgatgggg ggtactcggt
     1261 acgatcgctg ggctgatggg cctgaccggc tgcggttttg cgggcggcgc caccgaagtc
     1321 cgctacaagg tcacggtcga agtgctcgag aacggctcgg tccggtccgg gtcgtcggtc
     1381 tggagctggc ggctgagcga gccgaccgtg gcgctggcct ctccttatga cgggaagctc
     1441 cgcggcgagg cggtcgccgt cgagctgggc aatggccgca ccctgttcgc cgtccttcgc
     1501 ggcaccgacg gcgagagcgg catggcggag atgcttcccg agcggctgtt cggggacgtc
     1561 ggccgctcgg cgcgcggcga gcgcacccgc ttcagccccg acaggatcgc cgacctgcgc
     1621 gacatcgcgt cgcgggtcgg cgagacggcg acggttccgt gcgcggagcg tccgggctgg
     1681 tgcccgatgc tggtgacgtt ccggaatatc tccgacccga ccagcgtcga gcaggtcgat
     1741 cccaccgaca tcgccgcgac cctcggcccc ggctacgccc tcaaggcgat caccgtcgag
     1801 ctcgtctcgc aacgccccga gcatgcgatc gaggccaggc tgccctggct ggcggcggtc
     1861 ggccgggagc gcgcatcatt gatacccaat ccgccgcgtc tcctgaaaga cacgaagccg
     1921 atccagctag ttgccccctc agacttcagc acagagttgt acagatgaac cgcaagctct
     1981 ttttgtccat cctcgcgatg gactcctata atcgtgagta cggcgcaaac gtaagtctga
     2041 ccggcagcca aataggattg gcggacatcg tcacgcggaa ttcgcttggg ctgagcgacg
     2101 gcacctacaa cgcgtggcag gaaaagggtt tctacgcgag cgcatacgat gtcgacggcg
     2161 ccggcatcga cggcctcgtc ggaacggtca taagctacag gggcacgaac tttaactttt
     2221 cgtggctcaa cttcttcaac agcccgctgt ggcaggacat tcggagcggc tggtccgtcg
     2281 gcgcaggcgt ggccggggcc caagcgacca tggccctcga cttctaccac gcggtcaagg
     2341 gcgacggcgt cgatccccgc gacgcggaga tcacgaccac ggggcacagc ctcggcggcg
     2401 gcctcgcggg tctcgccgcc aatctctacg gccgcgaggc catcctcttc gatcacatga
     2461 cctacgggcc gatctcctcg acgatcaccc ggcaggccga gtcgagccag caggtgcgcg
     2521 atcgctattt cgagggccag tatccctggg aactggatcc gtccgggatt tcggccttct
     2581 tcacggaggg cgaaatactg aggctggaac gggcgctgtt cggcgagacg tcgtcgccct
     2641 tgcccagcca cagctcgggc cagagcgcgg tgaccctgca ctcgatggcg ctgctgacct
     2701 cgctcatctg ggcggagcag aacgccgatc cccgctggaa gacggccggc gcgctgctct
     2761 ggaacagctt tttcgacgag gccctcggga cgtcgcaggc gcttcccggc gtcgaatcgc
     2821 gcgcggggcc gggcggcacc ggcccggcgg tgatgaacag cgccatcgcc tattcggcgc
     2881 tcgacgcggg agagcgcccg ttcggcgaca cggcgatctg gtcgatgttc gacgatgccg
     2941 cgcaactggg cagcgtcctc ggcagcaccg gcggccccga tttcctcggc aagatcgtcg
     3001 acaccaaatt ctcgttcggc ctgggcggcc tcgacatcga ccgggtcacc gtccgccagc
     3061 acctcgccaa cctgctcgtc caatatgccg gagcgctggc cctgtacgac gtcgagcagg
     3121 agcagggcgg caccgttccg acgtcgagag gcccggtcga cgcccgggag ggcatcctgt
     3181 cccgcgagac catccagagc gctctcgccg tcgatctttc ccgaacgctc tggggcgacg
     3241 tcctcaagtc caatccgaac gcggtcacga agctccccgg ggaccggctc gagccgctgg
     3301 atcgcgacgc cttccgccag acctatttca agcaggcgga ggagcagggc atcctcgccg
     3361 gcgtccttcg cttcctcggc gtcaagagct cgaagtggga cgacgacgat cttcgggaga
     3421 tcacccgaca gctgtgggga accgacgacc tttcgatcct cgatcgcttc cacatggcgc
     3481 cggtcaacca gcccacgcac gtcatcctcg acaatcgagg ctacgtctcg gacacctcgg
     3541 agggcagcga cgcccaggtc gacgtctaca tcggcaccac cgccaacgag acgatcgaca
     3601 accggacgac cggccataac ctgatcatca ccggcgaggg cgccgacgat gccgacggcg
     3661 gc
//
LOCUS       DASVQZ010000372         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_944485, whole genome shotgun sequence.
ACCESSION   DASVQZ010000372 DASVQZ010000000
VERSION     DASVQZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944485"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..809
                     /locus_tag="VF547_10555"
     CDS             111..809
                     /locus_tag="VF547_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8263301.1"
                     /translation="MVLAAGLGKRMRPLTATRPKPLVEVAGKPLLDHCLDRLRAAGVE
                     KVVVNVHYLADSLEAHLNNRVEGLEIAVSDEREQLLETGGGLVRALPMIDADPFLVVN
                     SDNLWVDGPVDSLRLLAANWDEARMDALLLLVPLARAHCHKGQGDFHMSAAGALRRRK
                     PGSVAPFVYTGVQMVSKRLLEGEVPEGPFSTNLLWDRAIAKGRLFGAVHEGLWFDIGA
                     PPNIRKAEEILGLA"
     gene            831..>1677
                     /locus_tag="VF547_10560"
     CDS             831..>1677
                     /locus_tag="VF547_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEX8263302.1"
                     /translation="MIRPGVFTIPPHRAFADALAAGLSARFGGAETGLAQGIVLLPNN
                     RAVRAVTEAFVRRSGGGLLLPRLVPIGDPDLDERIGGALEPLGDGEMLPPAVEPLERL
                     FLLARLVQRHLGGIEAGEALRLAEDLARTLDQLIVEEVEPSRLRGFAAELPDLSLHWQ
                     SSLERLAVILDEWPRLLAERGRIDLADRRNRLLAAVAGRWRATPPPGFVAAAGITSPS
                     PAVARLLRVVSRLEKGLVVFPALDLDMPAEEWDSLGPHEPDETTGLRRRPIETHPQFH
                     LKRLLD"
BASE COUNT          221 a          632 c          577 g          247 t
ORIGIN      
        1 ccggcggaac agcgcggcga cgcgtgcttg cggaagatcg cggcaagagc cgccagcgcc
       61 ggccgctcgc gctccgcccg gtgccgagcg gcgaggtgcc gagaacggcg atggtgctcg
      121 ccgcggggct cggcaagcgc atgcgcccgc tcaccgcgac ccggcccaag ccgctggtcg
      181 aggtcgccgg caagccgctg ctcgaccatt gcctcgaccg gcttcgagcg gccggcgtgg
      241 agaaggtcgt cgtcaacgtc cattacctcg ccgattcgct cgaagcgcat ctcaacaacc
      301 gcgtcgaagg ccttgagatt gctgtatccg atgagcgcga acaattgctc gagaccggcg
      361 gggggctggt ccgggcgctg ccgatgatcg acgccgaccc cttcctcgtc gtcaattccg
      421 acaacctgtg ggtcgacggc ccggtcgatt cgctgcgcct gctcgccgcc aattgggacg
      481 aggcgcggat ggatgcgctg ctgctcctcg tcccgctcgc ccgcgcccat tgccacaagg
      541 gccagggcga cttccacatg agcgccgcgg gcgctctgcg gcggcgcaag cccggctcgg
      601 tcgcgccgtt cgtctatacc ggggtccaga tggtctcgaa gcgcctgctc gagggcgagg
      661 tgcccgaggg ccccttctcg accaacctgc tgtgggaccg agcgatcgcc aagggccggc
      721 tgttcggcgc cgtccacgaa gggctctggt tcgacatcgg cgctccgccc aacatccgca
      781 aggccgagga aatccttggg ctcgcctgac cgcatcgcta gcctcgggcg gtgatccggc
      841 ccggagtctt caccatcccg ccacaccgcg ccttcgcgga cgcgctcgcc gcggggctga
      901 gcgcccgctt cggcggcgcc gagacgggac tcgcccaggg catcgtcctc cttcccaaca
      961 accgcgccgt gcgcgccgtc accgaggctt tcgtgaggcg ctcgggcggc ggcctgctgt
     1021 tgccgcgcct cgtgccgatc ggcgacccgg atctcgacga gcggatcggc ggcgcgctcg
     1081 agccgctcgg ggacggggag atgctgccgc cggcggtcga gccgctcgag cggctcttcc
     1141 tgctcgcccg gctggtccag cgccacctcg gcgggatcga ggcgggggag gccctgcgcc
     1201 tcgccgagga tctggcccga accctcgacc agctgatcgt cgaggaggtc gagccgtcgc
     1261 ggctgcgcgg cttcgccgcc gagctgccgg acctctcgct ccactggcaa tcatcgctcg
     1321 agcggctcgc cgtgatcctt gacgaatggc cgaggctgct cgccgagcgc ggccggatcg
     1381 acctggccga ccgacgcaac cggctgctcg cggcagtcgc cgggcgctgg cgggccacgc
     1441 ccccgccggg cttcgtcgcc gccgccggaa tcaccagccc ctcgccggcg gtcgcgcggc
     1501 tcctgcgcgt ggtttcgcgg ctcgaaaagg ggctcgtcgt ctttcccgcg ctcgacctcg
     1561 acatgcccgc ggaggaatgg gactcgctcg gcccgcacga acccgacgag acgacgggcc
     1621 ttcgccgccg tccgatcgag acccacccgc aattccacct gaagcggctt ctcgacc
//
LOCUS       DASVQZ010000373         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_559366, whole genome shotgun sequence.
ACCESSION   DASVQZ010000373 DASVQZ010000000
VERSION     DASVQZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559366"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF547_10565"
     CDS             <1..500
                     /locus_tag="VF547_10565"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX8263303.1"
                     /translation="GAAMIALASIFAHPMPLAAVTALFGWWLFLLAALDVEQEWLPDR
                     LTWPLLAAGLAAAWGGFGPTLESRLIGAAAGFAGLFLIARLYRRLRGREGLGGGDPKL
                     LAAIGAWLGWTQLPFVLVGAGLLGLAALLVRRLRGEAVAPTDRLPLGALMALAAWPIW
                     IVIAR"
     gene            648..1478
                     /locus_tag="VF547_10570"
     CDS             648..1478
                     /locus_tag="VF547_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX8263304.1"
                     /translation="MRLAFDNRRLLGRVRGLNGWTVLELALLALIALQCARLIWTVLT
                     PVGPLGAWRASEGQGAASTSGLGEFDPFFRLSGTGGPVTVTSLNLKLFGIRQDQASGR
                     GSAIIAGSDGNQRSIAVGEEIEPGVTLKAVGFDSVTISRGGADEQLFMDQSQTPGTAP
                     PPGAPPAPAPPVVQPSTSVEPGPPVPPSKLYPNEVSMTPRMNGSQLTGVSVRPQGSGG
                     GFRALGLAPGDVVVAVNGRRIQTTEQARGVAAQLASARVTLQVERDGRVVTLRPGAAR
                     "
     gene            1505..>3283
                     /gene="gspD"
                     /locus_tag="VF547_10575"
     CDS             1505..>3283
                     /gene="gspD"
                     /locus_tag="VF547_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX8263305.1"
                     /translation="MLAAPPTPASAQHVVNFREADIRAFIEDAARVTGRTFIVDPAVN
                     GKVSVVTQRPLSRSEYFELFLSTLRANGLMAVPLQGGAFRIQPIATGAAAGGRVGGRG
                     SANNFVTEIFRTRNIEGAQALEALRPLISREGSITASRNSIVVADFADNVARVRQVLA
                     GIDRDTSTSRIVPLRNAGAREIATSLNALMEGSGGRPATIVPVDSSNSIAIRGDTSTV
                     ARLAAMAEELDQRAATGTEIRVIFLQHADAAQLLPVLQQLLGQPVTQPPAAPGLSRRD
                     ESSSSSRNVRQAGQPERTTTTSTTAPAPESATPTGVSIFGPRGAVVTRFQGANALVIA
                     ASTDVQRQLGEVIRQLDTRREQVLVEAIIVEISDNAAKKLGVQLLLAGLGGSNIPFAV
                     TNYSNASPNLLTVAGAVAAERLRTTTTTVNGQVVTTTQNSSVADALQEAAVNSVLGAT
                     GGIAGLAIRSGNALFGAIVNAVKSDSKSNILSTPSIMTLDNQEARILVGQEIPITTGE
                     ALSPNFDNQFRTVDRENVGITLEVLPQINAGGSIKLNLRQEVSSIAGPVSGRSADLIL
                     NKREIETVITVDDGEIVGIGGLLDDNE"
BASE COUNT          470 a         1184 c         1160 g          469 t
ORIGIN      
        1 tgggggcggc gatgatcgcg ctcgcctcga tcttcgccca tccgatgccg ctcgccgccg
       61 tcaccgccct gttcggctgg tggctgttcc tcctcgccgc gctcgacgtc gagcaggaat
      121 ggctgcccga ccggctgacc tggcccctgc tcgcggcggg gctggccgcc gcctggggcg
      181 gcttcgggcc gacgctggag agccggctga tcggcgcggc ggcgggcttc gccggcctgt
      241 tcctgatcgc ccgcctctac cggaggctgc gcggccgcga ggggctcggc ggcggcgatc
      301 ccaagctgct cgcggcgatc ggcgcctggc tgggctggac ccagcttccg ttcgtcctgg
      361 tcggggcggg ccttctgggc ctggccgcgc tgctggtcag gcggcttcgg ggcgaggcgg
      421 tggcgccgac ggaccggctg ccgctggggg ccctgatggc cctggccgcc tggccgatct
      481 ggatcgtcat cgcgcggtga ggaggattat ccacaagccg cttgcgcggc ggcgcaaaag
      541 ctggaagttt caccgcccgg cgaatctggg ctgaggtcgc caagatattg ttacgactgt
      601 ggaaaatcta ccgcgggtgc gctgcgcggg gaaggggttt ggggtagatg cgtttagcct
      661 ttgacaaccg gcggctgctc ggccgcgtcc ggggcctcaa cggctggacc gtgctcgagc
      721 tggccctgct cgcgctcatc gcgctgcagt gcgcgcgcct gatctggacc gtcctcaccc
      781 cggtcgggcc gctcggcgcg tggcgcgcgt ccgaagggca gggcgcggcc tcgacgtccg
      841 ggctcggcga gttcgatcct ttcttccggc tgagcgggac gggagggccg gtcacggtga
      901 cctccctcaa cctcaagctg ttcgggatcc gccaggacca ggcctccgga cgcggctcgg
      961 cgatcatcgc gggctccgac ggcaaccagc gcagcatcgc ggtcggcgag gagatcgagc
     1021 cgggcgtcac cctgaaggcg gtcggcttcg acagcgtcac catctcgcgc ggcggcgccg
     1081 acgagcaatt gttcatggac cagtcgcaga ctccggggac ggcgccgccg cccggcgcgc
     1141 cgccggcgcc ggcgccgccg gtcgtccagc ccagcacctc ggtggagccg gggccgcccg
     1201 tcccgccctc caagctctat ccgaacgagg tcagcatgac gccgcggatg aacggctcgc
     1261 agctgacggg agtctccgtc cgcccgcagg gcagcggagg cgggttccgg gcgctcggcc
     1321 tcgcccccgg cgacgtcgtc gtcgcggtca acggtcggcg gatccagacg acggagcagg
     1381 cgcgaggcgt ggccgcgcag ctggcctcgg cgcgggtgac cctccaggtg gaacgcgacg
     1441 gccgggtggt cacgctgcgt ccgggggcgg cccggtgaag cgcgcgctgc tggccctcgc
     1501 cctgatgctc gccgcgccgc cgactccggc gagcgcccag cacgtcgtca acttccggga
     1561 agcggacatc cgcgccttca tcgaggacgc ggcgcgggtg accggccgca ccttcatcgt
     1621 cgacccggcg gtgaacggga aggtttcggt ggtgacccag cggccgctgt cgcgctcgga
     1681 atatttcgag ctcttcctgt cgaccctgcg cgccaacggg ctgatggcgg tcccgctcca
     1741 ggggggcgcc ttccggatcc agccgatcgc caccggcgcc gccgccggcg gccgggtcgg
     1801 aggccgcggc agcgccaaca atttcgtcac cgagatcttt cggacccgca acatcgaggg
     1861 cgcgcaggcg ttggaggcct tgcggcccct gatcagccgc gagggctcga tcacggcgtc
     1921 gcgcaactcg atcgtcgtcg ccgacttcgc ggacaacgtc gcccgggtcc gccaggtcct
     1981 cgccggcatc gaccgcgaca ccagcacgag ccggatcgtg ccgctgcgca acgccggcgc
     2041 gcgcgagatc gcgacctcgc tcaatgcgct gatggaggga tcgggcgggc gcccggcgac
     2101 catcgtgcca gtcgacagct caaactcgat cgccatccgc ggcgacacct ccaccgtcgc
     2161 ccgcctcgcc gccatggccg aggagctcga ccagcgcgca gccaccggca ccgagatacg
     2221 agtcatcttc ctccagcatg ccgacgccgc gcagctgctc cccgtgctcc agcaactgct
     2281 cggccagccg gtcacccagc cgccggccgc gccgggcctg tcccgccgcg acgagagcag
     2341 ctcgagctcg aggaacgtcc gccaggccgg ccagccggag cgcaccacca cgacgagcac
     2401 gacggcgccc gcgccggaat cggcgacgcc gacgggggtg agcatcttcg ggccgcgcgg
     2461 cgcggtggtg acccgcttcc agggcgccaa tgcgctggtg atcgcggcgt cgaccgacgt
     2521 ccagcgccag ctgggcgagg tcatccgcca gctcgacacg cgccgcgagc aggtgctggt
     2581 cgaggcgatc atcgtcgaga tctcggacaa tgccgccaag aagctcgggg tccaattgct
     2641 tctcgccggc ctgggcggaa gcaacatccc gttcgcggtg accaattatt ccaacgccag
     2701 ccccaacctg ctcaccgtcg cgggagccgt ggcggcggag cggctgcgca cgaccaccac
     2761 cacggtcaat ggccaggtcg tcaccacgac ccagaattcg tcggtcgccg acgccctgca
     2821 ggaggccgcg gtcaattcgg tgctcggcgc gaccggcggc attgccggac tcgccatccg
     2881 cagcggcaat gccctgttcg gagcgatcgt caacgcggtg aagtccgaca gcaagtcgaa
     2941 catcctgtcg accccgtcga tcatgacgct cgacaaccag gaggcgcgga tcctggtcgg
     3001 gcaggagata ccgatcacga cgggcgaggc gctcagcccc aatttcgaca accagttccg
     3061 caccgtcgac cgcgagaatg tcgggatcac actcgaggtg cttcctcaga tcaacgccgg
     3121 cggctcgatc aagctcaacc tcaggcagga ggtgagctcg atcgcggggc cggtgtcggg
     3181 gcgctcggcc gatctcatcc tcaacaagcg cgagatcgag acggtgatca cggtcgacga
     3241 cggcgagatc gtcggcatcg gcggcctgct cgacgacaac gag
//
LOCUS       DASVQZ010000374         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_559401, whole genome shotgun sequence.
ACCESSION   DASVQZ010000374 DASVQZ010000000
VERSION     DASVQZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559401"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF547_10580"
     CDS             <1..202
                     /locus_tag="VF547_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263306.1"
                     /translation="PPSLAALEHRGRAAGADVRFVALDGDPAVLIKSVSYYSILYSND
                     KQNDSELGVRVVRALLLLDCRA"
     gene            complement(241..1404)
                     /locus_tag="VF547_10585"
     CDS             complement(241..1404)
                     /locus_tag="VF547_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263307.1"
                     /translation="MKRRPISAILRSPTLRRGLPWAALFLAGAAYGGAAVRYGLFPYP
                     DLQWQFHRLQRARQPMPVARKADLSLMTVATPADVERRRRQLVRLIWASDALPSRQPD
                     SVETGIRDPRFATLPNLARTDRLHVRMDFGLDSRILHFHPARANGVLVLVHDGHAQAT
                     GLVKMVARLLERGYSVAAFTMPLYGDNARPAVRAGQLGRIVLDNHEKLRLVRPAAGLT
                     AKYLIEPVPVVLNRLAPSYRSVAMVGLSGGGWTTTLAAALDPRIGLSFPVAGTSPPDI
                     ASPGAWHDFEQSDPALYVHVSYLDLYLLGAAGAGRGQLQILNEFDPCCFSGRAALSYR
                     AAVAARARALGGHYNLLIDNSHDQHQISDWAADRILAALAAGSSPAAGAASGR"
     gene            1542..>2104
                     /locus_tag="VF547_10590"
     CDS             1542..>2104
                     /locus_tag="VF547_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263308.1"
                     /translation="MTIKTMLFGFAALGVGGAALTGGGLGGGHDAERTIARPPAEVYA
                     AIASMATEGVVEREAEPGAPPVTFEVRKEPGKAVHYLLRIDGKVAGSVDLAVAPDKDG
                     AASRLSADIELDQAALRKLAGDGPEEFPTVPDALLDIAAGQMLGEMAARIEAGTPLEP
                     FGPDDMAAWQGHAPGAAEREATQAAAAA"
BASE COUNT          310 a          700 c          828 g          266 t
ORIGIN      
        1 tccgccgtcg ctggcggcgc tcgagcatcg cggcagggcg gccggcgccg acgtgcgctt
       61 cgtcgccctc gacggcgatc ccgccgtgct gatcaagagc gtctcctatt acagcatcct
      121 ctattccaac gataaacaga atgactcgga gctcggcgtc cgggtcgtcc gggcgctgct
      181 cctgctcgat tgccgggcct gaccgggggt cggcggggcg ccgcgccggc ggcggctccg
      241 tcaccggccg ctcgcggcgc cggccgccgg ggaggagccg gcggcgagcg cggccaggat
      301 ccggtccgcc gcccagtcgg agatctggtg ctggtcgtga ctgttgtcga tcagcaggtt
      361 gtaatggccg cccaatgccc gggcacgggc ggcgaccgcg gcccggtagc tcagggccgc
      421 gcggccggag aagcagcagg gatcgaactc gttcaggatc tgaagctggc cgcggcccgc
      481 gccggccgcg ccgagcagat acaggtcgag ataggagaca tggacgtaga gcgccggatc
      541 cgactgctcg aaatcgtgcc aggcgccggg agaggcgatg tcgggcgggc tggtgccggc
      601 gaccgggaag ctcagcccga tccgcgggtc gagcgcggcg gcgagggtcg tcgtccagcc
      661 gccgcccgac aggccgacca tcgccaccga gcgatagctc ggggcgagac ggttgaggac
      721 gaccggcacc ggctcgatca gatatttcgc ggtgaggccc gcggcgggtc ggacgaggcg
      781 cagcttctcg tgattgtcga ggacgatccg gccaagctgg ccggcgcgga cggccgggcg
      841 ggcgttgtcg ccgtacagcg gcatggtgaa ggcggcgacc gagtagccgc gctcgagcag
      901 ccgtgcgacc attttcacga ggccggtcgc ctgcgcgtgg ccgtcgtgga cgaggaccag
      961 gactccgttc gcgcgggccg ggtggaaatg gaggatccgc gagtccagtc cgaaatccat
     1021 ccggacgtgg aggcggtcgg tccgggcgag gttgggcagg gtagcgaagc gcgggtcgcg
     1081 gatccccgtc tccaccgagt ccggctggcg cgaaggcaag gcgtcgctcg cccagatcag
     1141 tcggacgagc tggcgccggc ggcgctcgac gtcggccggg gtggcgacgg tcatcagcga
     1201 caggtccgcc ttgcgagcga ccggcatggg ctggcgggcg cgctgcaggc gatggaactg
     1261 ccattgcagg tccggatagg ggaagaggcc gtagcgcacc gcggcgccgc cataagcggc
     1321 gccggcgagg aacagcgcgg cccacggcag gccgcgccgc agcgtcgggc ttcgaaggat
     1381 cgcgctgatc ggccgcctct tcatcggagc cactgtgtca gcgggggcgg ccggcgtcga
     1441 acgaaaaggg ccccggccgg ccgctgaaaa gagctcggtc cgcacatcct ttcttaaagc
     1501 ctcggcggcc atggtccggc ccctgcagaa ggggggaatc catgacgatc aagaccatgc
     1561 tgttcggctt cgccgcgctg ggggtcggcg gcgccgcgct gaccggaggc ggtttgggcg
     1621 gcgggcacga tgccgagcgg acgatcgcgc ggcccccggc cgaggtctat gcggcgatcg
     1681 cctcgatggc gacggaaggg gtggtcgagc gggaggccga gccgggcgcg ccgccggtga
     1741 cgttcgaggt ccgcaaggag ccgggcaagg cggtccatta cctgctccgg atcgacggca
     1801 aggtggccgg ctccgtcgac ctcgccgtcg cgcccgacaa ggacggggcc gcgagccggc
     1861 tcagcgccga catcgagctc gaccaggcgg cgctcaggaa gctcgccggc gacggccccg
     1921 aggaattccc gaccgtgccg gacgcgctcc tcgacatcgc ggccgggcag atgctcggcg
     1981 agatggcggc caggatcgag gccgggaccc cgctcgagcc gttcggcccc gacgacatgg
     2041 cggcctggca gggccatgcg cctggtgccg cggagcgcga ggcgacgcag gccgccgccg
     2101 ccgc
//
LOCUS       DASVQZ010000375         4083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_369915, whole genome shotgun sequence.
ACCESSION   DASVQZ010000375 DASVQZ010000000
VERSION     DASVQZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369915"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2378
                     /locus_tag="VF547_10595"
     CDS             <1..2378
                     /locus_tag="VF547_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8263309.1"
                     /translation="RYEMSYDKWLPGAENVYQIQSWYKSSETGQEAQLQMTPYLAGKR
                     IAKDFPQVEKEVYVNDNGPVFTKDGQATSTEDYLWVDNNFLEVIPLPMVHGNVGALSQ
                     PNTAVLTRSEAVKRFGTDNVVGRTMTLISRGVSRDFRITGVLEDLPKNSHLEINALAR
                     IDFVAYNADTPQVLDCWGCQNGYVYVKLRPGSDPAAIQAGFPAWEKRNIPDENAGEAR
                     FNAGDDQDWHIVNVRDVHLGRAQAGAMSPGNDRRTIVTFAIIALLILGMAVVNFTNLA
                     TARASQRAREVALRKVLGATRKQLIVQFIGESILIALVAMLIALAMVELLVRPFSTFL
                     EADLDLHYFGADGIALPALALVMVVGVLGGLYPAFFLSRFQPASVLKANKSSAETTGT
                     GRLRSLLVIAQFAVSIGLIICTAIIYGQTVYARTVDPGYNRSNILQVDELSRYALIDK
                     GEMIAERASRVPGVEAVGRTTIGVATDNNSNTGVMVPGNSEPVTVGTYQVDTGFFDAM
                     GLRLVAGRWFDKSRPLDDMTLPYPPDPASQRALAQRGGNIVVNELAAKRLGFERPADI
                     LNKTFRAAIVDNEIGLVPVTVIGVVKDSRFRSVKLPLDPIMFANARSGHSNMIVRYRG
                     DPATVRAGVERMWKGITSDVPFNAEFSDDIIGELYEAEDARAKTFAGFALLAVVVACL
                     GLFGLAAFTAERRTKEIGIRKVLGARSRDIVRLLAWQFSKPVIVANLIAWPIAWWVMR
                     DWLNGFEARIDLGPGPFLVAGILALVIALGTIAGHAFKVARSNPIHALRYE"
     gene            2387..>4083
                     /locus_tag="VF547_10600"
     CDS             2387..>4083
                     /locus_tag="VF547_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8263310.1"
                     /translation="MWRNYLTVALRALASNKVYAAINIGGLALGLAGCLLILAYVRYE
                     RSYDSWLAESGRVYQVQSKIHPPGQPDVLTQATPFPMDESLPGGFPQIEAVTSVSTGK
                     TVTERDGQPVFIDATTVDGDFFKVLALPFAQGSAATALPDSNSIVLTESEAIRQFGTA
                     AAMGRSFSLGAGPGKRDYKVSGVLRDLPRNTSLRVGIIYRRDLADIPPELRGWGNYDQ
                     LHYVRLRPGADAAAIDAGIPAWKKRVMTPEVIDGKAVSLADVFEFRLVPLRDVHLGET
                     QTAALTPGGDPRALATFAIVALLTLGMAVMNFVNLSTARATQRAREVALRKVLGARRR
                     QLIVQFLGESVLICAAAMLIALAIVEVAMPWVSRLTGTDMRVAYLGEGGMLLPALALL
                     AATGLGGGLYPAFYLSRFKPAPLLRAGPVSAETPGSGRLRVALVIGQFTVAIGLIAST
                     AVIWSQTRFVERVDPGYRRDGLVQIANAWRFTQGSEYEAARAEMLAIPGVTATARTGI
                     GLGSTETSIRLMRALGAPEHVSMGFHSIDSEYLRTMDIELLAGRLIGDRFAADRVTG"
BASE COUNT          638 a         1452 c         1344 g          649 t
ORIGIN      
        1 tccgctacga gatgagctac gacaaatggc tgcccggcgc cgagaacgtc taccagatcc
       61 agagctggta caaatcgagc gaaaccggcc aggaagccca gctccagatg accccctatc
      121 tggcgggcaa gcgcatcgcc aaggatttcc cgcaggtcga gaaggaggtc tacgtcaacg
      181 acaacggccc ggtcttcacc aaggacgggc aggcgacctc gaccgaggat tatctgtggg
      241 tcgacaacaa tttcctcgag gtgatcccgc tgccgatggt gcacggcaac gtcggcgcgc
      301 tcagccagcc caacacggcg gtgctgaccc gcagcgaggc cgtcaagcgc ttcggcaccg
      361 acaatgtcgt cggccggacg atgaccctga tcagccgcgg cgtctcgcgc gacttccgga
      421 tcacgggagt gctcgaggac ctgcccaaga actcccatct cgagatcaac gcgctcgccc
      481 ggatcgactt cgtcgcctac aatgccgaca cgccccaggt cctggactgc tggggctgcc
      541 agaacggcta cgtctacgtg aagctccggc cggggtcgga cccggcggcg atccaggcgg
      601 gcttcccggc ctgggagaag cgcaacatcc cagacgagaa tgccggcgag gcgcgcttca
      661 acgccggcga cgaccaggac tggcatatcg tcaacgtccg tgacgtccat ctcggccggg
      721 cgcaggcggg agcgatgtct cccggcaacg accgccggac gatcgtgacc ttcgcgatca
      781 tcgccctgct catcctcggc atggcggtcg tcaacttcac caacctcgcc accgcccgcg
      841 ccagccagcg cgcccgggaa gtggcgctgc gcaaggtgct tggagccacc cgcaagcagt
      901 tgatcgtcca gttcatcggc gaatcgatcc taatcgccct ggtcgcgatg ctgatcgcgc
      961 tggcgatggt ggagctgctc gtccggccct tctcgacctt cctcgaggcc gatctcgacc
     1021 ttcattattt cggcgccgac gggatcgcgc tgccggcgct ggcgctggtg atggtcgtgg
     1081 gcgtgctcgg cggcctctac ccggccttct tcctgtcgcg cttccagccc gcctcggtgc
     1141 tcaaggccaa caagtcgtcc gccgagacga cgggcaccgg acggctgcgc agcttgctcg
     1201 tcatcgccca gttcgcggtc tcgatcgggc tgatcatctg caccgcgatc atctacggcc
     1261 agaccgtcta cgcgcggacc gtcgatccgg gctacaatcg cagcaacatc ctccaggtcg
     1321 acgagctcag ccgctacgcg ctcatcgaca agggcgagat gatcgccgag cgagcgtcgc
     1381 gcgtcccggg agtcgaggcg gtcggccgga ccacgatcgg ggtcgcgacc gacaacaaca
     1441 gcaataccgg agtgatggtg cccggcaatt ccgagccggt cacggtcggc acctaccagg
     1501 tggatacggg cttcttcgat gcgatggggc ttcggctggt cgcgggccgc tggttcgaca
     1561 agagccggcc gctcgacgac atgaccttgc cctatccccc cgatccggcc tcgcagcggg
     1621 cgcttgcgca gcgcggcggc aacatcgtcg tcaacgagct ggcggcgaag cggctcggct
     1681 tcgagcggcc ggcggacatc ctcaacaaga cgttccgggc cgcgatcgtc gacaacgaga
     1741 tcggcctcgt cccggtcacc gtcatcggcg tggtcaagga ttcgcgcttc cggtcggtca
     1801 agctgccgct cgatccgatc atgttcgcga acgcgcgcag cggccacagc aacatgatcg
     1861 tccgctaccg cggcgatccg gcgacggtcc gggccggcgt cgagcggatg tggaagggca
     1921 tcaccagcga cgtgccgttc aacgccgagt tcagcgacga catcatcggc gagctgtacg
     1981 aggccgagga cgcccgggcc aagaccttcg ccggcttcgc cctgctcgcc gtcgtcgtcg
     2041 cctgcctcgg cctgttcggc ctcgccgcct tcaccgcgga gcggcggacc aaggagatcg
     2101 gcatccgcaa ggtgctcggc gcccgctcgc gcgacatcgt gcgcctgctc gcctggcaat
     2161 tctccaagcc ggtgatcgtc gccaacctga tcgcctggcc gatcgcctgg tgggtgatgc
     2221 gcgactggct gaacggtttc gaggcgcgca tcgacctcgg gccgggcccg ttcctggtcg
     2281 ccggcattct cgccctggtg atcgcgctcg gcaccatcgc cggccacgcc ttcaaggtcg
     2341 cccgctccaa cccgatccac gcgctgcgct acgagtagcc gggcggatgt ggcgcaacta
     2401 tctgaccgtc gcgctgaggg cgctggcgag caacaaggtc tacgcggcca tcaacatcgg
     2461 cggcctcgcg ctgggcctcg ccggctgcct gctcatcctc gcctatgtcc gttacgagcg
     2521 cagctacgac agctggctcg ccgagagcgg ccgggtctat caggtccagt cgaagatcca
     2581 cccgcccggc cagccggacg tgctgacgca ggcgacgccg ttcccgatgg acgaaagcct
     2641 tcccggcggc tttccgcaga tcgaagcggt gacctcggtg tcgaccggca agacggtgac
     2701 cgagcgcgac ggccagccgg tcttcatcga cgccaccacc gtcgacggcg acttcttcaa
     2761 ggttctcgcc ttgcccttcg cccaaggctc ggcggcgacc gcgcttcccg attccaactc
     2821 gatcgtgctc accgagagcg aggcgatccg ccagttcggc acggccgccg cgatgggccg
     2881 gtcgttcagc ctcggcgccg ggccgggcaa gcgcgactac aaggtcagcg gcgtcctgcg
     2941 cgacctgccg cgcaacacca gcctgcgcgt cgggatcatc taccgccggg acctcgcgga
     3001 cattccgccg gagcttcgcg gctggggcaa ttacgaccag ctccattatg tccggctccg
     3061 ccccggcgcc gatgcggcgg cgatcgatgc cgggatcccc gcctggaaga agcgggtgat
     3121 gacgcccgag gtgatcgacg gcaaggcggt gtcgctggcc gacgtcttcg agttccgcct
     3181 cgtgccgctg cgcgacgtcc atctcggcga gacccagacc gccgcgctca ctccgggcgg
     3241 cgacccgcgc gccctcgcca ccttcgccat cgtcgctctg ctgacgctcg gcatggcggt
     3301 gatgaacttc gtcaacctga gcacggcgcg ggccacgcaa cgggcgcgcg aagtggccct
     3361 gcgcaaggtg ttgggggcga ggcgccggca gctgatcgtc cagttcctgg gcgagagcgt
     3421 gctgatctgc gccgcggcga tgctgatcgc gctcgcgatc gtcgaggtgg cgatgccctg
     3481 ggtcagccgc ctgaccggca ccgacatgcg cgtggcctat ctcggcgagg gcggaatgct
     3541 tctgccggca ctcgccctgc tcgcggcgac cgggctgggc ggcggcctct atcccgcctt
     3601 ctacctcagc cggttcaagc ccgcgccgct gctgcgcgcc gggccggtct cggccgagac
     3661 gccgggcagc ggccggctcc gcgtggcgct ggtcatcggc cagttcaccg tcgccatcgg
     3721 gctgatcgcg agcacggccg tcatctggtc ccagacccgt ttcgtggagc gggtcgaccc
     3781 cggctaccgg cgcgacgggc tcgtgcagat cgccaatgcc tggcgcttca cgcagggatc
     3841 cgaatatgag gcggcgcgcg ccgagatgct ggccatcccc ggggtgaccg ccacggcccg
     3901 gaccggaatc gggctgggct cgacggagac atcgatccgg ctgatgcgcg cgctcggtgc
     3961 gcccgagcat gtctcgatgg gcttccacag catcgattcc gaatatctgc ggacgatgga
     4021 catcgagctg ctcgccgggc ggctgatcgg cgaccgcttc gccgccgacc gggtgaccgg
     4081 aag
//
LOCUS       DASVQZ010000376         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_848635, whole genome shotgun sequence.
ACCESSION   DASVQZ010000376 DASVQZ010000000
VERSION     DASVQZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848635"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VF547_10605"
     CDS             <1..855
                     /locus_tag="VF547_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263311.1"
                     /translation="LFQCTHGLGHGLTILHRHDLPQALRSCDLLSRPPEREMCYAGGF
                     MESLVNATHPHSLPGADKAKPGPKQKAAAAAAHAGHGAHGRHGPAVAEAGPAKPAFQA
                     LDPADLHYPCSRMEEKYLIACYMIQTSAMLHHSGRDVGRTAAECGRAPEKARVTCFVS
                     LGRDVSTIAVNDIPEAVRLCGLADKAFRTDCHLGVALSIVNMNANPAEAIPYCRALPE
                     PEGKRACYAAVGRQALGLPEGQAKREQACRLAEVELADVCLERPVPPRPAAGLPAAST
                     PGRADLPG"
     gene            894..>1816
                     /locus_tag="VF547_10610"
     CDS             894..>1816
                     /locus_tag="VF547_10610"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX8263312.1"
                     /translation="MPRPFAHRHLLGIDALSAEDILFLLDEAEPWLEFNRSARKADQR
                     LAGLTQFNLFFENSTRTLFSFEVAGKRLGAQVANFHPGASSVRKGESLIDTALTLGAM
                     RPDVMIVRHGETGAPHQVAEVVQCPVINAGDGTGEHPTQALLDALTMRRRKGRIEGLE
                     VAICGDVRHSRVAGSNLKLLPKLGARVRLVGPPGLLPADGGGLPVFTDLDRGIEGADV
                     VMMLRIQRERMQSGVEGSLAGYHARYGLTAARLARAAPDAVVMHPGPMNRGVEIDSDV
                     ADDPERSAILDQVELGVAVRMAVLDVLTRGRR"
BASE COUNT          266 a          689 c          617 g          244 t
ORIGIN      
        1 ctcttccagt gcacccacgg cctcggccac ggcctcacca tcctccaccg ccacgacctc
       61 ccgcaggcgc ttcgatcctg cgacctgctc agccgcccgc cggagcgcga gatgtgctac
      121 gccggcgggt tcatggagag cctggtcaac gcgactcatc cccatagcct gcccggcgcc
      181 gacaaggcca agccgggacc gaagcagaag gcggcggcgg cggccgccca cgccggccac
      241 ggcgcgcacg ggcggcacgg tccggcagtc gccgaagccg ggccggcgaa gcctgccttc
      301 caggcgctcg atccggccga cctccattat ccctgctcca ggatggagga gaaatatctg
      361 atcgcctgct acatgatcca gacctcggcg atgcttcacc acagcggccg cgacgtcggc
      421 cggacggcgg ccgaatgcgg tcgcgccccg gagaaggcgc gggtcacctg cttcgtcagc
      481 ctcgggcgcg acgtcagcac gatcgcggtc aacgacattc ccgaagccgt ccgcctgtgc
      541 ggcctggccg acaaggcgtt ccggaccgac tgccacctcg gagtcgccct gtcgatcgtc
      601 aacatgaacg ccaaccctgc cgaggccatc ccctattgca gggccttgcc cgagccggag
      661 ggcaagcgcg cctgctacgc cgcggtgggc cggcaggcgc tcgggctgcc ggaaggacag
      721 gcgaagcgcg agcaggcgtg ccgcctggcc gaggtcgagc tggccgacgt ctgcctggag
      781 cgtccggttc caccccggcc ggctgccggc ctcccggccg cgtcgacacc cggccgggcc
      841 gacctccccg gctgagcttg ccgcccatcc gccgccgctc tatcccggcg tcgatgcccc
      901 gccccttcgc gcaccgccac ctgctcggga tcgacgcgct gtcggccgag gacatcctct
      961 tcctgctcga cgaggcggag ccctggctgg agttcaaccg aagcgcgcgc aaggccgacc
     1021 agcgcctcgc cgggctcacc cagttcaacc tgttcttcga gaattcgact cggaccctgt
     1081 tctcgttcga ggtcgccggc aagcggctcg gcgcgcaggt cgccaacttc catcccggcg
     1141 cctcgagcgt gaggaagggc gagagcctga tcgacaccgc cctcaccctg ggcgcgatgc
     1201 ggcccgacgt gatgatcgtc cgccacggcg agaccggggc tccgcaccag gtcgccgagg
     1261 tcgtccaatg cccggtgatc aacgccggcg acggcaccgg cgagcacccg acccaggccc
     1321 ttctcgacgc cctcaccatg cgccgccgca agggccggat cgaagggctc gaggtggcga
     1381 tctgcggcga cgtccgccac agccgagtgg ccggatcgaa cctcaagcta ctgccgaagc
     1441 tcggcgcccg ggtgcgcctg gtcggcccgc ccggcctgct tccggcggac ggcggcggac
     1501 tgccggtctt caccgacctc gatcgcggaa tcgagggggc cgacgtggtg atgatgctga
     1561 ggatccagcg cgagcggatg caatccggcg tggagggcag cctggccggc tatcacgccc
     1621 gctacgggct gaccgcggcg cggctggcgc gggcggcgcc ggacgcggtc gtcatgcatc
     1681 ccgggccgat gaaccgcggc gtcgagatcg attccgacgt cgccgacgat ccggagcgct
     1741 cggcgatcct cgatcaggtc gagctcggcg tggcggtcag gatggcggtg ctcgacgtgc
     1801 tgacccgcgg ccgccg
//
LOCUS       DASVQZ010000377         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_754864, whole genome shotgun sequence.
ACCESSION   DASVQZ010000377 DASVQZ010000000
VERSION     DASVQZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754864"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VF547_10615"
     CDS             complement(<1..1035)
                     /locus_tag="VF547_10615"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEX8263313.1"
                     /translation="MTTHRRRRASRGALAAAAALAVTFAPLPGALPAAAQGAQQRPLY
                     KDPAQPVDRRVEDLIARMTLEEKAAQLVTVWEHKGKIQTPEGAFSAEKASQAFPNGLG
                     QIARPSDKRGVTVSNAAAGAAAGANREARDTADYVNAAQRWAVERTRLGIPILMHEEA
                     LHGYVARGATSFPQSIGLASSWDPELVERVFAVAAREMRARGTNLALAPVVDVARDPR
                     WGRIEETYGEDPYLVSEMGLAAIRGFQGRTLPLKADKVLVTLKHMTGHGQPENGTNVG
                     PAQISERTLRENFFPPFERAVKELPVRSVMASYNEIDGIPSHGNPWLLNDVLRGEWGF
                     KGAVVSDYFAI"
     gene            1150..2232
                     /locus_tag="VF547_10620"
     CDS             1150..2232
                     /locus_tag="VF547_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX8263314.1"
                     /translation="MARPSRRSRGSVTIEDVARAAGVSAMTVSRVMNGEKNVRESTRA
                     AVREAIERLRYSPNTAARSLAAGQATHIGLLYANPSDAYLSQFLVGAREGARRAGCHR
                     VIEACEYEQESEQAEAARRFATTQVEGVILPPPLSESVPILTELAAAEIPVVTVAMGR
                     LYENALNIRIDDFSAAREMTDYLLGLGHRRIGLIKGSPTQIATSERERGFKAALEAAG
                     LDPEGAPIEQGDFTFRSGLTAAERLLDRTDRPTAIFASNDDMAAAAVSVAHRRGLAVP
                     DDISIVGFDDTAPATTVWPELTTVRQPVSAMSEAALDLLLADLRSRRSGADTGPAERV
                     LDHELVVRESSGPAAKLRPAKTRKSA"
     gene            2341..>2822
                     /locus_tag="VF547_10625"
     CDS             2341..>2822
                     /locus_tag="VF547_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8263315.1"
                     /translation="MTEASPARANMGFIGAIVAVATIGGFLFGYDSGAVNGTQDGLRG
                     AFSLSEGGLGFTVGSLLIGCFIGAFLAGRLADIFGRRNVMMLAAVLFLVGALLQGLSE
                     SHLVFVVARILGGMAVGAASVLSPAYISEVAPAGIRGRMTTIQQIMIITGLTGAFLVN
                     "
BASE COUNT          408 a          993 c          985 g          436 t
ORIGIN      
        1 gatcgcgaaa tagtcgctga cgaccgcccc cttgaagccc cattcgccgc gaaggacgtc
       61 gttgagcagc cacggattgc cgtgggaggg gattccgtcg atctcgttgt aggacgccat
      121 caccgaccgg accggaagct ccttcaccgc gcgctcgaag ggcgggaaga aattctcgcg
      181 gagcgtccgc tcggagatct gggcggggcc gacattggtg ccgttctcgg gctggccgtg
      241 tccggtcatg tgcttgagcg tgaccagcac cttgtcggcc ttcagcggca gcgtcctgcc
      301 ctggaacccc cggatcgccg cgaggcccat ctcgctgacg agatagggat cctcgccata
      361 ggtctcctcg atccggcccc agcgcgggtc gcgggcgacg tcgacgacgg gcgcgagggc
      421 gaggttggtc ccgcgcgccc gcatctcgcg cgcggcgacc gcgaagaccc gctcgaccag
      481 ctcgggatcc cagctgctgg cgaggccgat cgattgcggg aagctggtgg cgccgcgggc
      541 gacatagccg tgcagggcct cctcgtgcat gaggattggg attccgaggc gggtccgctc
      601 caccgcccag cgctgggcgg cgttcacata gtcggcggtg tcgcgcgcct cccgattggc
      661 gcccgcggcg gcgccggcgg cggcgttgga gacggtgact cctcgcttgt ccgaggggcg
      721 ggcgatctgg ccgaggccgt tcgggaacgc ctgcgacgcc ttctccgcgg aaaaggcgcc
      781 ctcgggcgtc tggatctttc ccttatgctc ccagaccgtg acgagctgcg ccgctttctc
      841 ctcgagggtc atccgggcga tcagatcctc cacccgccgg tccaccggct gggccgggtc
      901 cttgtagagc ggccgctgct gcgcgccctg cgcggcggcc ggcagcgctc cgggcaaggg
      961 ggcgaacgtc acggccaggg cggcggcggc tgcgagcgcg ccgcgcgagg cgcggcgccg
     1021 acgatgagtc gtcatgatct tctcccgatc gcccttggag ggtctcttgt gatagcgcta
     1081 ccataaggct gcgcatccgc gcattgcaat ggggtagccg cccacctagg ctggctttgg
     1141 agggcattga tggcgaggcc gagccgccgc agccgcggca gtgttaccat cgaggacgtc
     1201 gcgcgcgccg ccggagtttc ggcgatgacc gtgtcgcgag tgatgaacgg cgagaagaac
     1261 gtccgggaaa gcacgcgcgc cgcggttcgc gaagcgatcg agcgcctcag atattccccc
     1321 aacacggcgg cccggagcct cgccgccggg caggcgaccc atatcggcct cctctacgcc
     1381 aatccgagcg acgcctatct cagccagttc ctggtggggg cgcgggaggg tgcgcgccgc
     1441 gccggctgcc accgcgtcat cgaggcctgt gaatatgagc aggagagcga gcaggcggag
     1501 gcggcgcggc gcttcgccac gacccaggtc gagggcgtga tcctgccgcc gcccctgtcg
     1561 gagtcggtcc cgatcctgac cgagctcgcc gccgccgaga tccctgtcgt caccgtcgcg
     1621 atggggcggc tgtacgagaa cgcgctcaac atccggatcg acgatttcag cgccgcccgg
     1681 gagatgaccg attacctgct cggcctcggg caccggcgca tcggcctgat caagggctcg
     1741 cccacccaga tcgccaccag cgagcgcgag cgcggcttca aggcggccct ggaggcggcc
     1801 ggcctcgacc cggagggagc gccgatcgag cagggcgact tcaccttccg ctcgggcctc
     1861 accgccgcgg agcggctgct cgaccgaacc gaccggccga cggcgatctt cgccagcaac
     1921 gacgacatgg cggcggcggc ggtgagcgtc gcccaccggc gcggcctcgc cgtccccgac
     1981 gacatcagca tcgtcggctt cgacgacacc gcccccgcca ccaccgtctg gccggagctg
     2041 accacggtgc gccagcccgt gtcggccatg tccgaggccg ccctggacct tctgctcgcc
     2101 gatttgcgca gccgccgctc gggcgccgat accggccctg ccgagcgcgt gctcgaccac
     2161 gagctcgtcg tccgcgaatc ctccgggccg gccgcgaagc tgcggccggc caagacccgg
     2221 aaatcggcct gatccctcga atcgggcaaa tcggagcgtg atcatctgcg aagttaacgc
     2281 taacatcttt gtcgagagcc gccggacgag tcggcccgaa cgttaggagg aggatcgtaa
     2341 atgaccgagg caagccccgc gcgggcgaac atgggcttca tcggcgcgat cgtcgcggtg
     2401 gcgacgatcg gcggcttcct cttcggctat gacagcggcg ccgtgaacgg gacccaggac
     2461 gggctgaggg gcgccttctc gctcagcgag gggggcctcg gcttcaccgt cggctcgctg
     2521 ctcatcggct gcttcatcgg cgcgttcctc gccgggcgcc tcgccgacat tttcggacgg
     2581 cgcaacgtga tgatgctcgc ggcggtgctg ttcctggtgg gggcgctgct ccagggcctg
     2641 tccgaatcgc acctcgtctt cgtcgtcgcc cgaatcctcg gcggcatggc ggtgggcgcg
     2701 gccagcgtgc tgtcccccgc ctacatctcc gaggtcgcgc ccgccggcat ccgcggccgg
     2761 atgacgacga tccagcagat catgatcatc accggcctca ccggggcgtt cctggtcaac
     2821 ta
//
LOCUS       DASVQZ010000378         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_754993, whole genome shotgun sequence.
ACCESSION   DASVQZ010000378 DASVQZ010000000
VERSION     DASVQZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754993"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF547_10630"
     CDS             complement(<1..201)
                     /locus_tag="VF547_10630"
                     /inference="COORDINATES: protein motif:HMM:NF036765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8263316.1"
                     /translation="MTIEAGRARGPGGWKVALGAGLIGAVIGGGAVAYGSRQSVRDYL
                     LENPEVLPEAMDRLRQKEAAKAI"
     gene            complement(191..1582)
                     /locus_tag="VF547_10635"
     CDS             complement(191..1582)
                     /locus_tag="VF547_10635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8263317.1"
                     /translation="MAEATVTLRRWGVLVARLALLVTLSFAIAARPAAAQSILRDAET
                     EALFRDMSRPLLEAAGLRPDNVQVVLIHDRSINAFVAGGQIVYIHSGLLAAASDANEV
                     QGVIAHEIGHITGGHIIRMQEGVKVATGIMILSMVLGAAAMAAGAGEAGMGLLAAGQQ
                     AAMGKFLAFSRTQESAADAAAAGFLAKSQTNGKGLLSFFKKLQNQEFRYAIPQDNGYA
                     RTHPLTGERLAALEQTVKNAPGWNKATDPRLEARFQRVKAKLSGFVEEPERTLVTYPE
                     SNKSVPARYARAYAWHKRAYPEKALAEADSLLAEQPNDPFFLELKGQILLESGRPDAA
                     LESLRQAVRHAPDQPLISALLGHALISTEKPGHFEEAKQVLRAAIGRDNSNPFAWYQL
                     GIVYDREGDPGRAALATAERYNLQGEPRLALVNAEQALKTVPTGTPDWLRAQDIAMVS
                     RTEVERKKKNRDD"
     gene            complement(1660..>3246)
                     /locus_tag="VF547_10640"
     CDS             complement(1660..>3246)
                     /locus_tag="VF547_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX8263318.1"
                     /translation="PALIHEDSNLIKRAIRDIYHREIEEVYVEGEDGYRAARAFMKLL
                     MPSHARRVKSYADPVPLFQRYGAEDQLTAMYDPVVQLKSGGYLVINPTEALVSIDINS
                     GRSTREHNIEQTAFATNIEAAHEIARQLRLRDMAGLVVIDFIDMESNGHIRKVERTMK
                     DALKNDRARIQVGRISSFGLMEMSRQRLRTGVLEASTRPCQHCEGTGLMRTAASAGLS
                     ALRMIEDEAGRGRGSRIILRAGREAAIYVLNKKRHEIDEVEQRYGVIVEVLIDEALEG
                     AKMSVESFGPPPAQRPRFEPIIEPAVDEEDLVEEMDEDEEEPEADEEDGEEAAESRQP
                     ERQQGRERGRDRERDDEGDRGRKRRRRRGRRGGRRRDGEEGGEPSALEPGAMTESEPA
                     EAAPVEEAEPQPAPEPGPEPEAEEKPKARRRPRARRKDEAPADRAEAEAPAASAPEAE
                     PTPAAEPVAAAEEPPAKPKRTRRKKAPEAEAAPAEAPAPEAANDRDSAATVEAESADS
                     GEAAEGGEPRRGWWQRTFGA"
BASE COUNT          396 a         1129 c         1152 g          569 t
ORIGIN      
        1 gatcgccttg gccgcctcct tctggcgaag gcggtccatt gcctcgggca ggacctcggg
       61 attttcgagc agatagtcgc gcaccgactg gcgcgatcca taggcgacgg cgccgccgcc
      121 gatcaccgcg ccgatcagcc ccgccccgag cgccaccttc cagccgccgg ggccccgggc
      181 ccggcccgcc tcaatcgtca cgattctttt tcttccgctc cacctcggtg cgcgagacca
      241 tcgcgatgtc ctgcgcgcgc agccagtcgg gcgtgccggt cgggacggtc ttcagcgcct
      301 gctccgcatt gaccagggcc agccgcggct cgccctggag gttgtagcgc tcggcggtgg
      361 cgagggcggc gcggccggga tcgccctcgc ggtcgtagac gatgccgagc tggtaccagg
      421 cgaagggatt ggaattgtcg cggccgatcg cggcgcgaag gacctgcttg gcttcctcga
      481 aatggcccgg cttctcggtg gagatgaggg cgtggccgag cagcgccgag atgagcggct
      541 ggtcgggcgc atggcggacc gcctggcgca gcgattcgag cgcggcgtcg gggcgtcccg
      601 attcgagcag gatctgaccc ttcagctcga ggaaaaaggg gtcgttgggc tgctcggcga
      661 gcaggctgtc ggcctcggcg agcgccttct ccggataggc gcgcttgtgc caggcatagg
      721 cccgggcgta gcgcgccgga acgctcttgt tgctctccgg ataggtgacg agggtccgct
      781 cgggctcctc cacgaagccg gagagcttcg ccttcacgcg ctggaagcgg gcctcgagcc
      841 gcgggtcggt ggccttgttc cagccgggcg cattcttgac cgtctgctcg agcgcggcga
      901 ggcgctcgcc ggtcagcgga tgggtgcgcg catagccgtt gtcctgcggg atggcgtagc
      961 ggaattcctg gttctggagc ttcttgaaga agctgagcag ccccttgccg ttggtctgcg
     1021 acttggcgag gaagcctgcg gcggccgcgt ccgccgccga ttcctgggtg cggctgaagg
     1081 cgaggaactt gcccatcgcc gcctgctggc cggcggcgag caggcccatg cccgcctcgc
     1141 cggcgcccgc cgccatcgcc gcggcgccga ggaccatcga caggatcatg attccggtgg
     1201 cgaccttcac cccttcctgc atccggatga tgtggccgcc ggtgatgtgg ccgatctcgt
     1261 gggcgatgac gccctgcacc tcgttggcgt cgctcgcggc ggcgagcagg ccggagtgga
     1321 tgtagacgat ctggcccccg gcgacgaagg cgttgatcga ccggtcgtgg atgaggacga
     1381 cctggacgtt gtcgggcctg agcccggcgg cttccagcag cggccgcgac atgtctcgga
     1441 acagggcctc ggtttcggcg tcgcgcagga tcgactgcgc ggcggccggc cgcgcggcga
     1501 tcgcgaagct caaagtgacg aggagggcga gccgggcgac caacacgccc cagcgtcgca
     1561 gcgtaaccgt agcttcagcc atgcgcgtcc tctgggccta gggggctgaa cctgacatga
     1621 gcgcgcccgc cggtgcaagc gcgctcatgc cggcctggtt caggctccga aggttcgctg
     1681 ccaccagccg cggcgaggct cgccgccctc ggcggcctcg cccgagtcgg ccgactccgc
     1741 ttcgacggtc gccgcgctgt cgcggtcgtt ggccgcttcg ggcgccggag cctccgcggg
     1801 cgccgcctcg gcctccgggg ccttcttgcg gcgggtccgc ttgggcttgg cgggcggctc
     1861 ctcggccgcg gcgaccggct cggcggcggg ggtcggctcg gcttccggag ctgacgccgc
     1921 gggagcctcg gcctcggccc tgtcggcggg ggcttcgtcc ttgcgccggg cgcgcgggcg
     1981 acggcgcgcc ttgggcttct cctctgcttc gggctcaggc cccggctcgg gggccggctg
     2041 aggctcggct tcttcgaccg gcgccgcttc cgcgggctcg gactcggtca tcgccccggg
     2101 ctcgagcgcc gagggttcgc cgccctcctc gccgtcccgg cggcggccgc cgcggcgtcc
     2161 gcggcggcgg cggcgcttgc ggccgcgatc gccctcgtcg tcacgctcac ggtcacggcc
     2221 gcgctcgcgg ccctgctgac gctccggctg gcgcgattcg gcggcttcct cgccatcctc
     2281 ctcgtcggcc tccggctcct cctcgtcctc gtccatctct tcgacgagat cctcctcgtc
     2341 gaccgcgggc tcgattatgg gttcgaagcg cgggcgctgc gccggaggtg gcccgaacga
     2401 ctcgacgctc atcttggcgc cttcaagcgc ttcgtcgatc agcacctcga caatcacgcc
     2461 gtaacgctgt tcgacttcgt cgatctcgtg gcgcttcttg ttgagcacgt agatcgccgc
     2521 ctcgcgcccg gcgcgcagga tgatccggct gccgcggccg cggccggcct cgtcctcgat
     2581 catgcgcagc gccgacaggc ccgccgatgc ggcggtgcgc atcaggcccg tgccttcgca
     2641 atgctggcaa ggcctggtgg acgcctcgag tacgccggtg cgcagccgct ggcggctcat
     2701 ctccatcagc ccgaaggacg agatgcggcc gacctggatg cgggcgcggt cgttcttgag
     2761 cgcatccttc atggtcctct cgaccttgcg gatgtggccg ttggattcca tgtcgatgaa
     2821 gtcgatgacg accaggcccg ccatgtcgcg cagcctgagc tggcgggcga tctcgtgcgc
     2881 agcctcgatg ttggtagcga acgcggtctg ctcgatattg tgctctcgcg tcgatctccc
     2941 tgagttgatg tcgatcgaca ccaacgcctc ggtcgggttg atgacgagat aaccgccgga
     3001 tttcagctgg acgactggat catacatcgc ggtcagctga tcctcggcgc catagcgctg
     3061 gaacagcggc accggatcgg catagctttt cacccggcgc gcgtggctgg gcatcagcag
     3121 cttcatgaag gcgcgggcgg cgcgatagcc gtcctcgccc tcgacataga cctcctcgat
     3181 ctcgcgatgg taaatgtcgc ggatcgcccg cttgatcagg ttcgaatcct cgtggatcag
     3241 cgccgg
//
LOCUS       DASVQZ010000379        18951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_657765, whole genome shotgun sequence.
ACCESSION   DASVQZ010000379 DASVQZ010000000
VERSION     DASVQZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 18951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18951
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657765"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF547_10645"
     CDS             <1..451
                     /locus_tag="VF547_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX8263319.1"
                     /translation="MRGGDRPKNAHTEFDEVSVMPRSQWEPWIIMRKPLEGRVQDNLR
                     KWKTGGFLRRSEAQPFGDVIKSAPTSKREREIAPHPSLKPQAFLREIVRASLPLREGK
                     VLDPFAGSGSTLAAANYIGYESVGIESDAKFVRLAERAIPKLAAISD"
     gene            complement(460..1026)
                     /locus_tag="VF547_10650"
     CDS             complement(460..1026)
                     /locus_tag="VF547_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263320.1"
                     /translation="MPLPKNLLPIHIEKAVGYVEREASELIDIYLEQANVFSAIVGIF
                     GVKGLDSFSPYKKHKHPDVAQQRFPDLSLHGKLNPPPQEALESKGSMRPWPVQSHYDH
                     PGWYIIWRYLVDTTQTIKPGFPVVIWRVDVAFLTKQNWKYEGSKAAEGQGGRTHTFGI
                     KEPKKTLNGCVAYALPGIAIKGGKPTLA"
     gene            1112..1513
                     /locus_tag="VF547_10655"
     CDS             1112..1513
                     /locus_tag="VF547_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8263321.1"
                     /translation="MPQFLAHIALVVRDYDEAIAWFTQKLGFALVADEYQSEQDKRWV
                     LVAPPGAGADATTILLARASSPEQAAFIGNQAGGRVFLFLATDDFDRDYAAYLARGVR
                     FVREPATRAYGKVAVFEDLYGSLWDLVQFAA"
     gene            complement(1538..1828)
                     /locus_tag="VF547_10660"
     CDS             complement(1538..1828)
                     /locus_tag="VF547_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8263322.1"
                     /translation="MIPVEISEAADADLVDILEFGTANFGLDRAEAYVASFQTTFDLI
                     SRHPLAGAVHDEVRPPIRSLPHGSHRVFYDVFEAEVVVQRILHKAVDVKRHL"
     gene            complement(1825..2067)
                     /locus_tag="VF547_10665"
     CDS             complement(1825..2067)
                     /locus_tag="VF547_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX8263323.1"
                     /translation="MAQMNVSIPDKLKGWAEQRVAEGRYSSTSDYIRDLVRRDQENEE
                     KLRRLQAAIDEGRASGVSERSIEDIIADNRKRLAAE"
     gene            2207..2626
                     /locus_tag="VF547_10670"
     CDS             2207..2626
                     /locus_tag="VF547_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263324.1"
                     /translation="MTASSPRRRVPAFSPVPLRYRRDGWTPGRQSDFLGRLAETGNVS
                     AAARHVGMTRESAYRLRDKPGAASFAAAWDSIVAQRPPAPMSTQDLLRRRAFYGALKP
                     ILRGGRHVATRHSPDDDAVLRLYRAEMRRRRTLRDRG"
     gene            2814..3398
                     /locus_tag="VF547_10675"
     CDS             2814..3398
                     /locus_tag="VF547_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEX8263325.1"
                     /translation="MPLFGRRKGSSALEALKGASWRCAGCELDHAGMFDLAAFAPDHW
                     EGPREYEPNSALRLDGDFLSEDFCVIAGEHFFVRCVYLIPVHGLESPFGFGIWSSLSR
                     RNLEIYVDGFDDGDFADWGPWSSWFANELKLFSGTRNQLCWMNPREERQRPCLSLDNP
                     DHPLAIAQRDGITAERVLEIYAAYGHLRQPPPAS"
     gene            3487..3933
                     /locus_tag="VF547_10680"
     CDS             3487..3933
                     /locus_tag="VF547_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263326.1"
                     /translation="MDPSDPSALIAELPDWNNGAGISAEAWIGCVGTFELAVGYSLVF
                     WPRFVRVDGLVLREGFSPDALRSCAAGGADPAGVEALMNHLHIADLHCGGASPSEEQL
                     RYLGRVLKEVHEAKLARDFPDLRFTVEFNDEPGLDPIDYQLTFWQP"
     gene            4175..4840
                     /locus_tag="VF547_10685"
     CDS             4175..4840
                     /locus_tag="VF547_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8263327.1"
                     /translation="MRRLALPVLAVLASCGPEAPEAERTGFASSTTGSIAKAEAPSGK
                     PRLAPFRAMTSDAEVLQGDPDVPGQPFVIRIRELPGTIVPPHSHPVDEHVTIVSGTWH
                     FGFGDAFDPARLTALPTGSYAFAPKGSTMFAYAPEGAVVQVHGIGPFHIEWKHGAATL
                     DEKPGLFRFRRGDRVTGPRGEGAIRQGYASGPLVQYEIEGPAGLFMAAESDLSRVAAA
                     RGD"
     gene            complement(4809..5543)
                     /locus_tag="VF547_10690"
     CDS             complement(4809..5543)
                     /locus_tag="VF547_10690"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEX8263328.1"
                     /translation="MSGRIVNPRAAVEIVRQGLERQPVIVIDDMLVEPDQWRQVAARA
                     RSGPIRPFHPGVSTPVPARLAGQLRDELEPLIGETFGLDPVPPASSCFLSLVTTRPEA
                     LVPVQRLPHVDGLERERIAILVYLAGTEKGGTAFFRQRSTGFETVDSGRLDAFQAALD
                     AGLAEHGEPPAGYLADDSELYDRIALYEARPNRGLVYRSHSLHSAAIVPGAELAEDPG
                     TGRLTLNAFLSPAVPRVNRPAQPPPG"
     gene            complement(5540..5935)
                     /locus_tag="VF547_10695"
     CDS             complement(5540..5935)
                     /locus_tag="VF547_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8263329.1"
                     /translation="MSDEDPSEAIDARIAALGDWRGEALARIRALIREADPDVVEAVK
                     WRKPSNPAGVPAWEHGGLICTGETYRDKVKLTFARGASLDDPSGLFNASLDGNTRRAI
                     DLREGDGLDGQAFMALVRAAVAANLSRGG"
     gene            6295..7107
                     /locus_tag="VF547_10700"
     CDS             6295..7107
                     /locus_tag="VF547_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8263330.1"
                     /translation="MNPNKALWEKGDFTRIAETMRASGEELLASLEVGPGLRMLDLGC
                     GDGTTALPAARAGADVLGVDIASNLVAAGNARAAAAGLANLRFEEGDASDLAGLADES
                     FDLVLTLFGAMFAPRPFDVAREMVRVARPGGRIVMGNWIPGDPTLVAQILKISSAYAP
                     PPPEGFVSPVTWGVEDQVRERFAAAGVAPADVSCERATWVFRHSGPPAEFLEMFRRFY
                     GPTMNAFDAAAKDGREKDLQAELAELFEAQNRAGPERTEIPATYLKVTVARK"
     gene            7201..8127
                     /locus_tag="VF547_10705"
     CDS             7201..8127
                     /locus_tag="VF547_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263331.1"
                     /translation="MIRSLPRLTPPVAAASLLLAACGGADGGPAAAPADERAAAGGNG
                     AAAAAPAETAAPAAASRWDLQSSGEGVALALLSGGDRAAIRLFCPAGRGRLLVNVPGF
                     RPVGSEERLSFGGGGDSVALVADAAGDRRRGGVSGSGPVPDELAALVGGPVSASYGAQ
                     RSGPHPAPPRTLAAAFVAACREEPQAAGPAVSSAPAGAGPCHMQDGERLRTSPLRAVG
                     TEPFWGARIEGRCVTYSHPDDQRGTRLWTRFAPGPDGGVWTGALAGRRFELRTRSRAG
                     CSDGMSDKAYPFAVELRIGAERRTGCAEPLER"
     gene            8174..8719
                     /locus_tag="VF547_10710"
     CDS             8174..8719
                     /locus_tag="VF547_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8263332.1"
                     /translation="MILGTPAAPVLETDRLILRGWRPEDFGAYAAMLADDETARFITV
                     QGRALSAGEAWNETAWLIGHWQMLGYGMFVVEERSSGAFCGRVGPLHPPRWPAFEMAW
                     SLAPAARGKGYATEAARAAIGWSFETLPLDRIVSIIDPRNEASRRVAERLGQRRTGER
                     FTPFSDPCDVWEIRREDWPTP"
     gene            complement(8752..9204)
                     /locus_tag="VF547_10715"
     CDS             complement(8752..9204)
                     /locus_tag="VF547_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8263333.1"
                     /translation="MAEMSLAEISKKLRDIDFTILSTRTGGGAIAGRPMSNNRDVDFD
                     GDSWFFTCDDTRTVADIVRDPHVGLGYQGKSGALGFKPFFVSVEGRAELIRDRARFAE
                     HWTKDLDRWFEQGIDTPGLVLIKVTAQRLHYWDGYDEGELNLAEREEA"
     gene            complement(9279..9680)
                     /locus_tag="VF547_10720"
     CDS             complement(9279..9680)
                     /locus_tag="VF547_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263334.1"
                     /translation="MARFALRFAGLPAALCLQMVAGAPFLAPAPALAASRPQQMGGTW
                     DLSWKSRRGNRKTGYMVVEQRGSELVAQVYDRGGATATGSIAGTTFTLRGSRLAIPFT
                     VTGRIKGGRMTGILSALGTERRFTGIRRGRR"
     gene            complement(9777..10430)
                     /locus_tag="VF547_10725"
     CDS             complement(9777..10430)
                     /locus_tag="VF547_10725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8263335.1"
                     /translation="MIRQIGLFPLLALALAGCGDAPAPSAPTTANEATSAKAPQKPMG
                     DVVRVRLDTEAGPILLALDSKRAPVTAANFVRYVDSNRFDGTFFYRAARTKGARDRGF
                     VQGGIRHSARRAFPPIEHEPTGLTGLRHVDGAVSMARAAPSGAMGDFFIIVGGAMPAM
                     DSKDGEPGFAVFGRVEQGMDVVRKILDAPTVPNAGRGPMRGQMIEKPVKIVAAERVE"
     gene            complement(10548..11309)
                     /locus_tag="VF547_10730"
     CDS             complement(10548..11309)
                     /locus_tag="VF547_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263336.1"
                     /translation="MKKILFALAATTALAAAAPAAAQYGMAGHGQIRSDQLQLQLQAG
                     VRSGAISRAEAMPLREQLRQLTRLETLYARDGISGRERADLQHRINSLRQGIRMAARN
                     GDGRFDRDDDRYDHDGRYSRADERYDRDNDGWDDRDGNRDGRWDVDARNDSNRDGWDD
                     RDFDRDGRWDNDEGYARDRVDDRGVVGQIIDRVTGTGGLRVGQRASADLGALPYAYRS
                     RYHDNDSAYYRSDGRQIYEIDARTQTVVRVYAMNR"
     gene            complement(11430..12872)
                     /locus_tag="VF547_10735"
     CDS             complement(11430..12872)
                     /locus_tag="VF547_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8263337.1"
                     /translation="MGYQRFGTALALLALLSSCGGGGGGGAPPPPPAPPVGSVPPPPP
                     PTASTCSLRTRQDWAASVLREWYLFPETLPASLDPAGYTSVEAYIDALTATARAQRKD
                     RFFTHLASIAVEDAFFSSGSSAGFGVRLSYETVQGRVFIAEAFEGAPALAAGIDRGTE
                     IIAIGTNAGDMRTVASLMATGGAQAVSDALGPSSPGVTRVLRVTDSAGTRDVTVAKAD
                     YTLQPVSSRYGARVLDDGGRRVGYLNLRTFISTADPALRNAFATFRAQGITDIVVDLR
                     YNGGGLVSTAELMGDLLGRDRSTSDVLSFTTFRPEKSSYNETRFFQPQPQSVAPTRIA
                     FIGTTGTASASELVINAFIPYLHARAALVGTNTYGKPVGQIAIDRSACDDRLRVVAFK
                     TENAARQGEYFDGLASKVEASCQAGDDLSRPFGDPQEASIKAALDFLAGRACTRISSG
                     GITTQAVRERRELLTPERPSTVQREVPGAF"
     gene            12993..13352
                     /locus_tag="VF547_10740"
     CDS             12993..13352
                     /locus_tag="VF547_10740"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX8263338.1"
                     /translation="MTGSGESVFLFSYGTLRQAEVQRATFGRLLEGRPDVLPGFALSP
                     MTITDPHVVATSGASVHTIARPTADPEDRIAGLVFTVTREELDSADRYESGPIRRISA
                     RLASGLEAFVYVDARPE"
     gene            complement(13410..14075)
                     /locus_tag="VF547_10745"
     CDS             complement(13410..14075)
                     /locus_tag="VF547_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263339.1"
                     /translation="MKKILLSMAAVGALAAAAPAAAQSGYYGTNTNASFGISNQIAQL
                     EARLQAGVQSGLIDRTEARELRMDLRDLRRLERQYSYNGLTQAERQDLRQRVRSLRQE
                     IRMADNGRYDRDNRFGNWNDPYWNDGYTGQGGPYEQPYNYDCDTNSRGGIGGVIDSVL
                     GRDNCGLRVGARATGNLYSVPSQYRYQYRDGNGVYYRSDGRSIYQIDARTNTILRIYP
                     MDR"
     gene            14199..14666
                     /locus_tag="VF547_10750"
     CDS             14199..14666
                     /locus_tag="VF547_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263340.1"
                     /translation="MRTLLFLGAAGLLAACGPRESGGSAGASTGPTPATATPAETAVP
                     PAPAEAEPIVAAAIPGPFLGVYDASREACGRSSDARLTVSAGELRFHESIATVRKVTP
                     VGADSVRVEADYQGEGERWRNVRDLRLGAGGTELTVSGDGTRFTRVRCPAATR"
     gene            14675..15631
                     /locus_tag="VF547_10755"
     CDS             14675..15631
                     /locus_tag="VF547_10755"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX8263341.1"
                     /translation="MRILFLLGALFLLATFAPPSLQRRDPPPAVSLIHFAPVALDGER
                     PGSTRLGRLTFLGGWALTSNDARFGGISALHVEAGEALALSDAGWRIRFPLPGRRSPV
                     RAEILMIEKGPGPDGEKENRDVESLAIAGDDLWIAYEQANSIWRYRRRDFAPRASARP
                     PAMAEWNSNRGAEAMARLADGRFLVFDEGSGGDSQALLFAGDPAVAGTASRRLRYRPP
                     AGYRVTDAALLPDGRLALLHRRVGLFEGFTAKLTIAAPPAAAQGDLLSGEEAASFAEP
                     VTRDNFEALSVTREGGRTILWIASDDNYNALQRTLLMKFALD"
     gene            complement(15657..15953)
                     /gene="rpmB"
                     /locus_tag="VF547_10760"
     CDS             complement(15657..15953)
                     /gene="rpmB"
                     /locus_tag="VF547_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX8263342.1"
                     /translation="MSRICELTGKGRQVGHNVSHANNKTKRTFLPNLQNVTLISEALG
                     RGIKLRVSMNGLRSVEHVGGLDNWLLKSKDDSLSLKARRLKREIAKKQSEASAA"
     gene            16135..16383
                     /locus_tag="VF547_10765"
     CDS             16135..16383
                     /locus_tag="VF547_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263343.1"
                     /translation="MLRAVLMIVALLIILAIGAVWLGLVDLNQTQNAQAPKFEVKVNP
                     VTVGTTTANIQTPTVEMKTKQVDVPTVQVGGGDQANHQ"
     gene            16421..16876
                     /locus_tag="VF547_10770"
     CDS             16421..16876
                     /locus_tag="VF547_10770"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX8263344.1"
                     /translation="MTAAFSEPMRLALEEARAAAAAGEVPVGAVVTRAGTVIAAGRNR
                     MRADNDPTAHAEMVAMRSAAAALGVPRLDGCDLWVSLEPCAMCAGAMALARISRLYFA
                     ASDPKGGAVLHGPRLFAQPTCHHAPEVYSGMGEAEAGQLLRSFFRARRL"
     gene            17053..18384
                     /locus_tag="VF547_10775"
     CDS             17053..18384
                     /locus_tag="VF547_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX8263345.1"
                     /translation="MPPTSPLPPFPWFDVVVILMLIALNGVFAMAEMAIVSSRKPRLN
                     AMAKSGRWGAQTALDLAADPGKFLSTVQIGITLIGILAGAYSGASLGGPVGGRLERLG
                     LEPETAATIGFAIVIILTTYASLVIGELVPKQFALRSPEPLAAFMGPPMLWIARATAP
                     LVWLLDRTSALIFRLLRMRRDNANKVTAEELHLVVAEAHSAGVLEEYERAIISGVVRL
                     ADRPAREVMTPRTEVAWIDARAGPEEIREALLQTPHSRIPVADGSVDQLIGIVQARDI
                     VAALIEGRPLDLRALSREAPVVPDQMDAMDALAVLRGSQTRMAFVHDEYGHFEGIVTS
                     ADLLNAISGVLAGGFEGGPEAPLVEREDGSWWVSGSLSADALSDRLGIGLPEDRDYAT
                     TAGFALSVLKHIPEVGERFTFGGWQFEIVDMDGFKIDKLLASEVVRKRRGG"
     gene            complement(18502..>18951)
                     /locus_tag="VF547_10780"
     CDS             complement(18502..>18951)
                     /locus_tag="VF547_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263346.1"
                     /translation="NLILLVVALAIGLAVALWAFRGRGSGAADGPEGNALPDEFAAAT
                     QDVAGEILGVDAHPRIPDPSGPPDDLRLLKGVGPKLAAQLNAFGITRFDQLAGLTENE
                     VALLDRRMGAFEGRIARDRLVEQACYLQRGDTDGFEAKFGKLGGGQT"
BASE COUNT         2712 a         6692 c         6546 g         3001 t
ORIGIN      
        1 tatgcgtggc ggtgaccgac ccaagaacgc gcacactgaa ttcgatgagg tttcagtgat
       61 gccgcgctca cagtgggaac cttggatcat aatgcgtaaa ccgctcgagg gtcgcgttca
      121 ggacaacctc agaaaatgga agactggagg ctttctccgg cgttcagagg cacaaccttt
      181 tggggacgtg atcaagagtg cgccaacgtc gaagcgcgag cgcgaaatcg cgccccatcc
      241 ttccctcaag cctcaggcgt tccttcgcga aatcgtccga gcctcgctcc cgttgcgaga
      301 gggaaaggtg ctcgaccctt tcgcggggtc cggctcaact ttggctgcgg cgaattacat
      361 tggctacgaa agcgtcggaa tagaaagcga cgcgaaattc gtccgcctag ccgagcgagc
      421 tattccgaag ctagcagcca tctctgactg acgagaggat cacgccagcg ttggcttacc
      481 gccctttata gcaatcccag gtagggcgta agcgacgcag ccgttgaggg tcttttttgg
      541 ctctttgatg ccaaaggtgt gtgttcgacc accttggcct tcggccgcct tgctaccttc
      601 gtacttccaa ttctgctttg ttaagaatgc gacatcaact cgccatatca cgaccgggaa
      661 tccgggtttg atggtttgcg ttgtatccac caaatatcgc cagatgatgt accatccagg
      721 atgatcataa tgcgactgaa ctggccacgg cctcatgctg cctttcgact caagcgcctc
      781 ttgcggaggc ggattaagtt tcccgtgtaa tgataagtcg ggaaagcgct gctgggccac
      841 atccgggtgc ttatgcttct tgtagggact aaaagagtcc agacccttca ccccgaaaat
      901 ccctacgatg gcgctgaaca cattagcttg ttcgaggtat atgtcgatta gctccgaggc
      961 ctcacgctca acgtatccga cagccttctc gatgtggatc gggagaagat tcttaggtaa
     1021 cggcacgctg gtcccccgcg ggttacccga ttgtggcgat cgggatgagg tttggcaagc
     1081 ctgaagctaa cagggccagg atctaagaag gatgccccaa ttcctcgccc acatcgcgct
     1141 cgtcgtccgc gactatgacg aggcgatcgc ctggttcaca caaaagctcg gcttcgcgct
     1201 cgtcgcggac gagtaccagt ccgagcagga caaacgctgg gtgctcgtcg cgccgccggg
     1261 cgccggcgcg gacgcgacca cgatcctcct cgcccgcgcc tcctcgcccg agcaggccgc
     1321 gttcatcggc aaccaggcgg gtgggcgggt cttcctgttc ctcgccacgg acgatttcga
     1381 ccgcgactac gcggcctacc tcgcccgcgg agtccgcttc gtccgcgagc ccgcgacccg
     1441 cgcctacggc aaggtcgccg tgttcgagga cctctacggc agcctctggg acctggtgca
     1501 gttcgccgcc tgaaccctcg cgcctccgcg tgaaccctca aaggtgccgt ttcacatcca
     1561 ccgccttgtg gaggattctc tgaacgacga cttccgcctc gaagacgtca tagaagaccc
     1621 tgtgactgcc gtgcggaagg cttcggatcg ggggccggac ctcgtcatgc acggcgcctg
     1681 ccagcggatg ccgggagatc aggtcgaacg tcgtttggaa gctggccaca taggcttcgg
     1741 cgcggtccag gccgaaattg gcggtgccaa attcgaggat atccacgagg tcggcatcgg
     1801 ccgcctcgct tatctccacc gggatcattc agcggcgagg cgcttacggt tatcggcgat
     1861 gatatcctcg atcgagcgct cgctcacccc ggaagctcgc ccctcgtcga tcgccgcctg
     1921 gaggcgcctg agcttctcct cattctcctg gtccctccgg acgaggtcgc ggatatagtc
     1981 gctcgtgctg ctgtagcgcc cctcggcgac tcgctgctcc gcccagccct tgagcttgtc
     2041 ggggatcgag acgttcatct gtgccatgcg cttcctatac gattttgcgc caagattgcc
     2101 aaatcatcag aacaggctga agcataggcg gaccggatcc cctgtcggta cctttagcgg
     2161 cgcgtcgcct gttgactttc tgttcctgat atgttctaat ccggccatga ccgcctcatc
     2221 cccccgccgc cgggtcccgg ccttcagccc cgtcccgttg cgctaccgcc gcgacggctg
     2281 gactccgggg cggcagtcgg atttcctcgg ccgtctcgcc gagaccggga acgtcagcgc
     2341 ggccgcccgc cacgtcggga tgacccggga gagcgcctat cgccttcgcg acaagccggg
     2401 cgccgccagc ttcgccgccg cctgggactc gatcgtcgcc cagcggccgc ccgccccgat
     2461 gtccacacag gacctgctcc ggcgccgcgc cttctacggc gccctgaagc cgatcctgcg
     2521 cggcggccgg catgtcgcaa cccgccattc cccggacgac gacgccgtcc tcaggctgta
     2581 ccgagccgag atgcgccgcc gccgcaccct tcgggacagg ggctaaggtc acaggcgaaa
     2641 aaatgggcga atcgacttca gtcgtgacct tccggcccgc gccgccgccg agcccttcga
     2701 agcgtccacc gggcgagggc gcgggcaggc gcgttcacgg ctcgcggtcg aaatgctacg
     2761 ccctcccgga tgcggctccg cgcggaacgc cgccctgcaa taggagcatg gccatgcctc
     2821 tgttcggtcg acgcaagggc tcctcggcgc tcgaggcgct gaagggcgcc tcgtggcgct
     2881 gcgccggttg cgagctcgac catgccggga tgttcgatct cgccgccttc gcgcccgatc
     2941 actgggaggg gccgcgggaa tacgagccga actcggcgct tcgcctcgac ggcgacttcc
     3001 tgtccgagga tttctgcgtg atcgccggcg agcatttctt cgtccggtgc gtctacctga
     3061 tcccggtcca cggcctcgaa agccccttcg gcttcggcat ctggtcgagc ctctcccgac
     3121 gcaatctcga gatctatgtc gacggcttcg acgacggcga tttcgcggac tggggaccgt
     3181 ggagcagctg gttcgccaac gagctcaagc tcttctccgg cacccgcaac cagctgtgct
     3241 ggatgaatcc ccgcgaggaa cggcagcgcc cctgcctgtc cctggacaac ccggaccatc
     3301 cgctggcgat cgcccagcgc gacgggatca ccgccgagcg cgtgctcgag atctacgccg
     3361 cctacggcca tctgcgccag ccgccgcccg cttcctgacg ccgccgccgg cccgcatcgc
     3421 ccgcgccgtc cgcggcgatc ggcgagccct tgaacgaggc ccgcgattgc tgcgagaacg
     3481 gctcagatgg acccgtccga tccctccgcc ctgatcgccg agcttcccga ctggaacaac
     3541 ggcgccggca tctccgccga ggcctggatc ggctgcgtcg gcacgttcga gctggcggtc
     3601 ggctacagcc tcgtcttctg gccgcgcttc gtcagggtcg acgggctcgt cctgcgggag
     3661 ggcttcagcc cggacgccct gcgcagctgc gcggccggcg gcgccgaccc ggcgggcgtc
     3721 gaggcgctga tgaaccatct ccacatcgcc gacctgcatt gcggcggcgc ctcgcccagc
     3781 gaggagcagc tgcgctacct cggccgggtg ctgaaggagg ttcacgaggc gaagctcgca
     3841 cgggacttcc ccgacctgcg gttcaccgtc gagttcaacg acgagccggg cctcgacccg
     3901 atcgactacc agctcacctt ctggcagccc tgaacgcccg cccacccgcg agggcctggc
     3961 cgggaggcgc ggccggtccg agccgccgcc cggcgccgcg gcaaccgggt cgacagcggc
     4021 tcggggacgg ccttggccgg ggtggcgctt cacgctacgg tcctcccatg cgcccgcccg
     4081 gcctccgctc ccggcccggc cgatccgatt cggccgcctt gcgatgttcc gcggcccgcc
     4141 cgggcgcctc cgacaagagg ggagggagcg gctgatgcga cgcctggccc tcccggttct
     4201 ggccgtgctc gcgtcgtgcg gcccggaggc gccggaggcc gagcgcaccg gcttcgcctc
     4261 ctcgacgacc gggagcatcg cgaaggccga ggccccgtcc ggcaagccgc gcctcgcgcc
     4321 gttccgggcg atgacgtccg acgccgaggt tcttcagggc gatccggacg ttcccggcca
     4381 gcccttcgtc atccgcatcc gcgagctgcc cgggacgatc gtgccgcccc attcgcaccc
     4441 ggtcgacgag catgtcacca tcgtctcggg cacctggcat ttcggcttcg gcgacgcctt
     4501 cgatcccgcc cggctgacgg cgcttcccac cggcagctac gccttcgcgc ccaagggcag
     4561 cacgatgttc gcttacgctc cggaaggggc ggtggttcag gtccacggca tcggtccctt
     4621 ccacatcgaa tggaagcacg gcgccgcgac tctcgacgag aagcccggcc tgttccgctt
     4681 ccggcgcggc gaccgggtga cgggcccgcg gggcgagggc gcgatccgcc agggctacgc
     4741 gtccggtccc ctcgtccagt acgagatcga gggcccggcg ggcctgttca tggccgccga
     4801 gagcgacctt agccgggtgg cggctgcgcg gggcgattga ccctgggcac ggccgggctg
     4861 aggaaggcgt tgagcgtgag gcgtcccgtc cccgggtcct ccgccagctc cgcgcccggg
     4921 acgatggcgg cggagtggag gctgtggctg cgatagacga ggccgcggtt cgggcgcgcc
     4981 tcgtagagcg cgatgcggtc gtaaagctcg gaatcgtcgg ccagatagcc cgccggcggc
     5041 tcgccatgct cggcgaggcc ggcgtcgagc gcggcctgga aggcgtcgag gcgcccggag
     5101 tcgaccgtct cgaagccggt cgagcgctgg cggaagaagg cggtgccgcc cttctccgtg
     5161 ccagcgaggt agacgaggat cgcgatccgt tcccgctcga gcccgtcgac atgggggagg
     5221 cgctggaccg gcaccagcgc ctcgggccgg gtcgtcacca gcgacaggaa gcagctcgac
     5281 gccggcggca ccggatcgag cccgaacgtc tcgccgatca gaggctcgag ctcgtcgcgc
     5341 agctgaccgg cgagccgggc ggggaccggc gtgctgactc cgggatggaa gggccggatc
     5401 gggccggaac gggcccgcgc ggcgacctgc cgccattgat cgggctcgac cagcatgtcg
     5461 tcgatgacga tcaccggctg ccgctcgagg ccctgccgga cgatctcgac ggccgctcgg
     5521 ggattgacga tccgcccgct catccgcctc gcgacaggtt cgccgccacc gccgcccgga
     5581 ccagcgccat gaacgcctgg ccgtcgagac catcgccttc gcgcaggtcg atcgcccggc
     5641 gcgtgttgcc gtcgaggctg gcgttgaaca ggcccgaagg atcgtcgagc gacgcgcccc
     5701 gcgcgaaggt cagcttcacc ttgtcgcggt acgtctcgcc ggtgcagatc aggccgccat
     5761 gctcccaggc cggaacgccc gcgggattgg aaggcttgcg ccatttcacc gcctccacca
     5821 cgtccggatc ggcctcgcgg atcagcgccc ggatccgcgc cagggcttcg cccctccagt
     5881 cgcccagcgc ggcgatccgg gcgtcgatcg cctcggatgg atcctcgtcg ctcatcctcg
     5941 cctccctctt gccgcggccg ccgctccccg cgggcggtcc ggccatcctc gacccggacg
     6001 gcctgctgcc ggtcattgcg ggcctcggcc gacaggcgga tagataatgc gaaccgacac
     6061 cgcaacgcgc ctcgttccgg cattccgccg aagagcatgc cgccggcctc gcttcgtcgc
     6121 cggccaatcc cgcccgcctc gccgccgccg ccgggggctg gcaacgatcg ccctgccacg
     6181 atcgccacct ttctcgctgc ggcgcggcat cgaaccgtca cagacggcct tcagggcagc
     6241 cgatccggcg gccgaagccg cacaaggccc cgacatcaga aggaagcccg gccgatgaac
     6301 cccaacaagg ccctgtggga gaaaggcgat ttcacccgga tcgccgagac gatgcgagcg
     6361 agcggcgaag agctcctcgc ctcgcttgaa gtcggtcccg gcctcaggat gctcgacctc
     6421 ggctgcggcg acggcaccac cgcgctcccc gccgcccgcg ccggcgccga cgtgctcggg
     6481 gtcgacatcg cctcgaacct ggtcgccgcc ggcaacgccc gcgccgccgc ggccggcctc
     6541 gccaacctgc gcttcgagga aggcgacgcc tcggacctgg ccgggctcgc cgacgagagc
     6601 ttcgatctcg tcctcaccct gttcggagcg atgttcgccc cgcgtccgtt cgacgtcgcg
     6661 cgcgagatgg tccgggtcgc ccgtcccggc ggccgaatcg tcatgggcaa ttggatcccg
     6721 ggcgatccga ccctggtcgc ccagatcctg aagatcagct cggcctacgc gccgcccccg
     6781 cccgaaggct tcgtcagccc cgtcacctgg ggagtggagg accaagtccg cgagcgcttc
     6841 gccgccgccg gcgtcgctcc ggcggacgtc tcgtgcgagc gcgcgacctg ggtcttccgc
     6901 cattccggcc cgccggccga gttcctcgag atgttccgcc gtttctacgg cccgaccatg
     6961 aacgccttcg acgccgccgc gaaagacggc cgcgagaagg atctgcaggc ggagctggcc
     7021 gagctgttcg aagcgcagaa ccgggcgggc ccggagcgca ccgagatccc cgccacctat
     7081 ctgaaggtga cggtggccag gaagtgacgg ctcacgggcg ggtcgaagcg cgcaagcgcc
     7141 tgccgagcgg cacccgtgca acggcggaac cttcccgcgc ccgcgggcct tgatgctccg
     7201 atgatccgga gccttcctcg cctgacgccg cccgtcgctg ccgcgtcgct gctccttgcg
     7261 gcctgcggcg gcgccgacgg cggccccgcg gcggcgccgg ccgacgagcg ggccgccgcc
     7321 ggcgggaacg gcgcggccgc cgccgctccg gccgagacgg ccgctccggc cgcggcctcc
     7381 cgctgggacc tgcagtcgag cggggagggc gtcgcgctgg ccttgctgtc gggcggcgac
     7441 agagccgcga tccgtctgtt ctgcccggcc gggcggggca ggctgctggt caacgttccc
     7501 ggattccgtc cggtcgggag cgaggagcgg ctgtcgttcg gaggcggcgg cgactccgtc
     7561 gcgctcgtcg ccgacgccgc gggcgaccgc cggcgcggcg gggtgagcgg ctccgggccg
     7621 gtcccggacg agctcgccgc gctggtcggc ggtcccgtct cggcgagcta cggcgcgcag
     7681 cgcagcggac cccatccggc gccgccgcgg accttggccg ccgccttcgt cgccgcctgc
     7741 cgggaagaac cgcaggcggc cgggcccgcc gtgtcctcgg ctcccgccgg tgccggcccc
     7801 tgccatatgc aggacggcga gcggctgcgc acgtcgccgc tgcgcgcggt gggcaccgag
     7861 cctttctggg gcgcgcggat cgagggacgg tgcgtcacct actcccaccc cgacgaccag
     7921 aggggcaccc gcctctggac ccgcttcgcg ccgggtcccg acggcggcgt ctggaccggc
     7981 gcgctggccg gccggcgctt cgagcttcgg acccgcagcc gggcgggctg ctcggacggg
     8041 atgtccgaca aggcctatcc gttcgccgtg gagctgcgga tcggcgccga gcggcgcacc
     8101 ggctgcgccg agccgctcga gcgatagcgt ccttcgcccg aattgcgcca catgccgctg
     8161 ccaggctcgc gccatgatcc tcggaacccc cgccgcgccc gtgctggaga cggaccgcct
     8221 catcctccgc ggctggcggc cggaggattt cggcgcctat gccgcgatgc tcgccgacga
     8281 tgagacggcc cgcttcatca ccgtccaggg ccgcgccttg tccgccggcg aggcctggaa
     8341 cgagacggcg tggctgatcg gccactggca gatgctcggc tacggcatgt tcgtcgtcga
     8401 ggagcggtcc agcggcgcct tttgcggccg ggtcgggcct cttcacccgc cgcgctggcc
     8461 cgccttcgaa atggcctggt cgctggcgcc tgcggcccgc ggcaagggct atgcgaccga
     8521 ggcggcgcgc gccgccatcg gctggtcgtt cgaaaccttg ccgctcgacc ggatcgtgtc
     8581 gatcatcgac ccgcgcaacg aggccagccg gcgcgtcgcc gagcggctcg ggcagcgccg
     8641 caccggcgag cgcttcacgc cgttcagcga cccctgcgac gtctgggaga tacggcgcga
     8701 ggactggccg accccctaaa ggggcaccac ccccgccggc agtggacgcc gctaggcctc
     8761 ctcccgctcc gccaggttca gctcgccttc gtcgtaaccg tcccaataat gcagccgctg
     8821 cgccgtcacc ttgatcagca cgaggccggg ggtgtcgatg ccctgctcga accagcggtc
     8881 gaggtccttc gtccaatgct cggcgaagcg cgccctgtcg cggatcagct cggcccgccc
     8941 ttcgaccgag acgaagaacg gcttgaagcc gagcgctccg gacttgccct ggtagccgag
     9001 gccgacgtgc ggatcgcgga cgatgtcggc gacggtgcgc gtgtcgtcgc aggtgaagaa
     9061 ccaggagtcc ccgtcgaaat cgacgtcgcg gttgttgctc atcgggcgcc ccgcgatggc
     9121 gccgccgccg gtccgggtgg aaaggatggt gaagtcgatg tcgcgcagct tcttcgaaat
     9181 ctcggcgagg ctcatttcgg ccatgtcggg ctcctgattg ggatcaggtc cccaacgcgc
     9241 cgcggccacc gcggttcagc ctggctccac gacccttctt agcgacggcc gcggcggatg
     9301 ccggtgaaac gacgctccgt tcccagcgcg ctcaggatgc ccgtcatccg gccgcccttg
     9361 atccggccgg tcaccgtgaa gggaatggcc agccggctgc cgcgaagggt gaaggtcgtg
     9421 cctgcgatcg aacccgtcgc cgtggcgcct ccccggtcgt agacctgcgc gacgagctcc
     9481 gagccgcgct gctcgaccac catgtagccc gtcttcctat tgcccctcct cgacttccag
     9541 ctcaggtccc aggtgccgcc catctgctgg ggcctcgagg cggccagggc gggcgcgggc
     9601 gccaggaagg gcgcgccggc caccatctgg aggcatagcg cggccggcag gcccgcgaag
     9661 cggagggcaa atcgggccaa gcttctgtcc gccacggcgg catctcctga tcaaggtcgg
     9721 gtgtccttaa cccggcgagc gcccaaaggt tcagcccgga gcgccgctga gccgggctat
     9781 tccacccgct cggcggcgac gatcttcacc ggtttctcga tcatctggcc ccgcatcggc
     9841 ccgcggccgg cattggggac ggtcggggcg tcgaggatct tgcggacgac gtccatgccc
     9901 tgctcgaccc tgccgaagac cgcgaagccc ggctcgccgt ccttcgagtc catcgccggc
     9961 atggcgccgc cgacgatgat gaagaagtcg cccatcgcgc cggaaggggc cgcgcgcgcc
    10021 atggagacgg cgccgtcgac gtgcctgagg ccggtcaggc cggttggctc gtgctcgatc
    10081 ggcgggaagg cccggcgggc gctgtggcgt atgccgccct ggacgaagcc gcggtccctc
    10141 gcgcccttgg tgcgcgcggc gcgatagaag aaggtgccgt cgaagcggtt cgagtcaacg
    10201 tagcgcacga agttggcggc ggtcaccggc gcccgcttcg aatcgagcgc gaggaggatc
    10261 ggcccggcct cggtgtcgag ccggactcgg acgacgtcgc ccatgggctt ttgcggcgcc
    10321 ttggcggagg tcgcttcgtt cgccgtggtc ggtgcggagg gcgccggcgc gtcgccgcat
    10381 ccggcgaggg cgagcgcgag cagcgggaaa aggccgatct gccgaatcat gggtccactg
    10441 tggcccgtcg ccgtcctaga ctcaaaccac ggcgtcgcat gaaagcgaag ggcgccccga
    10501 ccggaccggt cggagcgccc ttgcgatcac ggcgcgccga accggtgtca gcggttcatc
    10561 gcatagacgc ggaccaccgt ctgggtccgg gcgtcgatct cgtagatctg gcgtccgtcc
    10621 gagcggtagt aagcggagtc gttgtcgtga tagcggctgc gataggcata gggcagggcg
    10681 ccgagatcgg ccgacgcgcg ctgtccgacc cgaaggccgc cggtgcccgt cacccggtcg
    10741 atgatctggc cgacgacgcc gcggtcatcg acccggtcgc gggcatagcc ctcgtcattg
    10801 tcccagcgtc cgtcgcgatc gaagtcgcgg tcgtcccagc cgtcgcggtt cgaatcgttg
    10861 cgggcatcga cgtcccagcg gccgtcgcgg ttgccgtcgc ggtcgtccca gccgtcgttg
    10921 tcgcggtcgt agcgttcgtc ggctcggctg tagcggccgt catggtcgta gcggtcgtcg
    10981 tcgcggtcga agcggccgtc gccgtttcgc gccgccatgc ggatgccctg gcgaagggag
    11041 ttgatccggt gctggaggtc ggcgcgctcg cggccgctga tgccgtcgcg ggcatagagc
    11101 gtctcgagcc gggtcagctg gcgcagctgc tcgcgcagcg gcatcgcctc ggcccgcgaa
    11161 atcgcgccgc tcctgacgcc ggcctggagc tgcagctgca gctggtcgga acggatctgg
    11221 ccgtggccgg ccatgccata ttgggcggcg gccggggcgg cggcggcaag ggctgtggtc
    11281 gccgccaggg cgaataggat cttcttcatt tgggtatctc ctgttgagcg ctccccgacg
    11341 cttgcctcac cctggcgccc gccgcggtga ggcccgcctg aacggcgtcc acaggtgagg
    11401 ttcatcgccg gccgcgttcg gccgggcgct cagaacgcgc ccggaacctc gcgctgcacc
    11461 gtgctcggcc gctccggagt gagcagctcg cggcgctcgc gcaccgcctg ggtggtgatt
    11521 ccgcccgagc tgatccgggt gcaggcccgt ccggcgagga aatcgagcgc cgccttgatc
    11581 gacgcttctt gcgggtcgcc gaacggccgg ctgaggtcgt ccccggcctg gcagctcgct
    11641 tcgaccttgc tcgccaggcc gtcgaaatac tcgccctgcc gggccgcgtt ctcggtcttg
    11701 aaggcgacga cccgaagccg gtcgtcgcag gccgagcggt cgatggcgat ctgaccgacc
    11761 ggcttgccgt aggtgttggt gccgaccagc gcggcccggg cgtgcagata ggggatgaag
    11821 gcgttgatca cgagctcgct cgccgatgcc gtgccggtgg tgccgatgaa ggcgatccgg
    11881 gtcggcgcca ccgattgcgg ctgcggctgg aagaatcggg tctcgttgta cgacgacttc
    11941 tcgggacgga aggtggtgaa gctgagcacg tcgctggtcg agcggtcccg gccgagcagg
    12001 tcgcccatca gctccgcggt cgaaaccagc ccgccgccgt tgtagcgcag gtcgacgacg
    12061 atgtccgtga tgccctgcgc ccggaaggtc gcgaaggcgt tgcgcagcgc cggatccgcg
    12121 gtcgagatga aggtgcgcag gttgagatag ccgactcgcc gcccgccgtc gtcgagcact
    12181 cgggcgccgt agcgcgagga gaccggctgc agcgtgtaat cggccttggc gacggtgacg
    12241 tcgcgggtgc cggcggaatc ggtgactcga agcacccggg tgacgccggg cgacgacgga
    12301 ccgagcgcat cgctgaccgc ctgcgcgcct ccggtcgcca tgagcgaggc cacggtgcgc
    12361 atgtcgccgg cattggtgcc gatcgcgatg atctcggtgc cgcggtcgat tcccgcggcc
    12421 agcgccggcg cgccctcgaa cgcctcggcg atgaagaccc gcccctggac ggtctcgtag
    12481 gagagtcgaa ccccgaagcc cgcgctcgat cccgagctga agaaggcgtc ctcgacggcg
    12541 atcgaggcga gatgggtgaa gaaacggtcc ttgcgctgcg cccgcgccgt cgccgtcagg
    12601 gcgtcgatat aggcctcgac gctggtgtag ccggccgggt cgaggctggc cgggagcgtc
    12661 tcggggaaca ggtaccattc gcgcaggacg gaggcggccc agtcctggcg ggtgcgaagc
    12721 gagcaggtgg aggcggtcgg cggcggaggc ggaggcaccg agccgaccgg cggggcggga
    12781 ggcggcggag gcgcgccgcc gccacccccg ccgccgcacg aagacaaaag ggcgagcagc
    12841 gccaacgccg tcccgaaccg ctgatatccc atccccgaat ccccgagctt cccagtttct
    12901 tcaactattt agcctacacg tgggctaatg ccagcctcta ttttgcgggc gggggccgga
    12961 ggcgctatcg aggcgccgtc cctggagcga ccatgaccgg cagcggcgaa tccgtcttcc
    13021 tcttctccta cggcacgctt cgccaggccg aggtgcagcg cgccacgttc ggccgcctgc
    13081 tggaggggcg gccggacgtg ctccccggct tcgcgctctc gccgatgacg atcaccgatc
    13141 cccacgtcgt cgccacgagc ggcgcgtcgg tccacacgat cgctcggccg accgccgacc
    13201 cggaggaccg gatcgccggc ctcgtcttca ccgtgacccg cgaggagctc gactcggccg
    13261 accgctacga atcggggccg atccggcgga tctcggcccg gctcgcctcg gggctcgaag
    13321 cgttcgtcta cgtcgacgct cgacctgaat gaaagaaggg cgccccggcg gttgccgggg
    13381 cgccccttcc tcaaatcggt ccgagccgat cagcgatcca tcggatagat tctcaggatc
    13441 gtgttggtgc gggcgtcgat ctggtagatc gaccggccgt ccgaccggta atagacgccg
    13501 ttgccgtccc gatattggta gcgatattgc gacggcaccg aatagaggtt gccggtcgcg
    13561 cgggcgccga ccctcagccc gcaattgtcg cggccgagga cgctgtcgat gactccgccg
    13621 atgccgccgc gactattggt gtcgcaatca taattgtagg gctgctcgta ggggccgccc
    13681 tggccggtat agccgtcgtt ccaatagggg tcgttccagt ttccgaagcg gttgtcgcgg
    13741 tcgtagcggc cgttgtcggc catccggatc tcctggcgca gcgagcgcac ccgctggcgc
    13801 aggtcctgcc gctccgcttg ggtcaggccg ttatagctgt actggcgttc gagccgcctc
    13861 aggtcgcgca gatccatgcg cagctcgcgc gcctcggtgc ggtcgatcag gcccgactgg
    13921 acgccggcct ggaggcgggc ctcaagctga gcgatctggt tcgagatgcc gaagctggcg
    13981 ttggtgttgg tgccgtaata gccgctctgg gccgcagcag gcgcggctgc cgcgagcgcg
    14041 cccacggcgg ccatcgaaag aagaatcttt ttcattacac atactccact aggccccccg
    14101 cttcgcgcat cccaacgttt gggcggcgcg ggtgttgcgt ttttgcggta aggcgatgct
    14161 ttggcgccac ggacggcgcg aaatgaagga atcaggcgat gcggaccctt ttgttcctgg
    14221 gcgcggccgg gctgctcgcc gcctgcggcc cgcgcgaaag cggagggtcg gccggcgctt
    14281 cgaccgggcc gacgccggcc actgccacgc ccgccgagac cgctgtgccg ccggcgccgg
    14341 ccgaggccga gccgatcgtg gccgccgcca ttcccgggcc cttcctcggc gtctacgacg
    14401 cgtcacgcga ggcctgcggc cggtcgagcg acgcccggct caccgtgtcg gcgggcgagc
    14461 ttcgcttcca cgagagcatc gcaacggtgc gcaaggtgac gcccgtcggc gccgactccg
    14521 tgagggtcga ggccgactac cagggcgagg gcgagcgctg gcgcaacgtc cgcgaccttc
    14581 ggctgggcgc cggcgggacc gagctgacgg tcagtgggga cggcacccgt ttcacccggg
    14641 tgcgctgccc ggccgcgaca cgctaggcgc gagagtgcgt atcctgttcc tcctcggcgc
    14701 gctcttcctg cttgccacct tcgcgccgcc gagcctgcag cgccgcgacc cgccgccggc
    14761 ggtgagcctg atccatttcg ccccggtcgc gctcgacggc gagcggcccg gctcgacccg
    14821 gctcgggcgc ctgaccttcc tcgggggctg ggcgctgacc agcaacgacg cccgcttcgg
    14881 cggcatctcc gctctgcacg tcgaggccgg agaagcgctg gcgctcagcg acgccggctg
    14941 gcggatacgc tttccgcttc ccggccggcg cagcccggtc cgggccgaga tcctgatgat
    15001 cgagaagggc ccggggccgg acggggaaaa ggagaatcgc gacgtcgaat cgctggcgat
    15061 cgcgggggac gacctgtgga tcgcctacga gcaggccaat tcgatatggc gctaccggcg
    15121 aagggacttc gccccccgcg cctccgccag gccgcccgcc atggcggagt ggaactcgaa
    15181 ccgcggcgcc gaggcgatgg cgcgcctcgc cgacggccgc ttcctggtct tcgacgaagg
    15241 aagcggaggc gacagccagg ccctgctctt cgccggcgat ccggcggtgg ccgggacggc
    15301 gtcgcggcgg ctgcgttacc gtccgccggc cggttaccgg gtcaccgacg ccgcgctgct
    15361 gcccgacggc cggctcgccc tgctccaccg ccgcgtcgga ctgttcgagg gcttcaccgc
    15421 caagctgacc atcgcggcgc cgccggcggc ggcgcagggc gatctcctat cgggagagga
    15481 agcggcgagt ttcgcggagc cggtgacgcg cgacaatttc gaggcgctga gcgtgacccg
    15541 ggaaggcggc cggacgatcc tgtggatcgc ctccgacgac aattacaacg cgcttcagcg
    15601 caccctgctg atgaagttcg cgctggactg agccgcccca ccgccggggc gacggatcaa
    15661 gccgccgacg cttccgactg cttcttggcg atctcgcgct tcaggcggcg ggccttgagg
    15721 ctcagcgaat cgtccttcga cttgagcagc caattgtcca ggccgccgac atgctcgacc
    15781 gagcgcaggc cgttcatcga cacgcgaagc ttgatgccgc ggcccagcgc ctcgctgatc
    15841 agcgtcacgt tctgcagatt gggcaggaag gtgcgcttgg tcttgttgtt ggcgtgggat
    15901 acgttgtggc ccacctgccg gcccttgccg gtcagctcgc aaatgcgcga catgatgtct
    15961 cgtcctgatt tgaagcgggg gacgcgaagg tccccggaaa ccgggggcgg atagccgccg
    16021 cgggggtttt cgtcaacctc tcgcgccggt cgggcgtcgg gcgaggaggt cgttgcggag
    16081 gtgcaaccga atcgcctacc cgttggttgt cggacgaagg aggaagcata acccatgtta
    16141 cgcgctgtcc tgatgatcgt cgccttgctc atcatcctcg ccatcggcgc ggtctggctc
    16201 ggcctcgtcg atctcaacca gacccagaac gcccaggctc cgaaattcga ggtgaaggtc
    16261 aatccggtga cggtgggcac caccaccgcc aacatccaga ctccgaccgt cgagatgaag
    16321 accaagcagg tcgacgtgcc gacggtccag gtcggcggcg gcgaccaggc gaatcaccaa
    16381 taggcttcca gcccttgcgg gccgggtcta ggccggccga atgaccgcgg ccttctccga
    16441 accgatgcgc ctggcgctcg aagaggcgcg ggcggccgcc gccgccgggg aagtgcccgt
    16501 cggggcggtg gtgacccggg ccgggacggt gatcgccgcc ggccgcaacc ggatgcgcgc
    16561 cgacaacgac cccaccgccc atgccgagat ggtcgcgatg cggtccgccg ccgcggcgct
    16621 cggcgtcccg aggctcgacg gctgcgacct gtgggtgtcg ctcgagccct gcgcgatgtg
    16681 tgcgggcgcg atggcgctgg cccggatctc acgcctctat ttcgccgctt ccgatccgaa
    16741 gggcggcgcc gtcctccacg gcccgcgcct cttcgcccag cccacctgcc accatgcgcc
    16801 ggaggtctat tcgggcatgg gggaggccga ggcggggcag ttgctccggt ccttcttccg
    16861 agcgcgcagg ctctaattcc tcccccggat ttccggggga gggggaccat gcgaagcatg
    16921 gtggaggggt ctttcggact cgaagaaccc ctccaccgcg ctccgcgcgg tccccctccc
    16981 cgcgaaatcg cggggaggaa ttagggcgcc cttgttccct ctcccccttc gcggctaagg
    17041 agggccggcg ccatgccgcc cacctccccc ctgccgccct tcccctggtt cgacgtcgtc
    17101 gtcatcctga tgcttatcgc gctgaacggg gtcttcgcga tggccgagat ggcgatcgtc
    17161 tcgtcgcgca agccgcggct caacgcgatg gccaagtcgg gacgatgggg cgcccagacg
    17221 gcgctcgacc tcgccgccga tccggggaag ttcctctcca ccgtccagat cgggatcacc
    17281 ctgatcggga tcctcgccgg cgcctattcg ggcgccagcc tcggcgggcc ggtcggcgga
    17341 cggctggagc ggctcggcct cgagccggag acggccgcga cgatcggctt cgccatcgtc
    17401 atcatcctca ccacctatgc ctcgctggtg atcggggagc tcgtcccgaa gcagttcgcc
    17461 ctgcgctcgc ccgagccgct ggcggccttc atggggccgc cgatgctgtg gatcgcaagg
    17521 gcgacggcgc ccctggtctg gctgctcgac cgcaccagcg ccctcatctt ccgcctgctc
    17581 aggatgcgcc gtgacaatgc caacaaggtg acggcggagg agctgcacct ggtcgtcgct
    17641 gaggctcaca gcgccggcgt gctcgaggaa tatgagcggg cgatcatctc gggcgtggtc
    17701 cggctggccg accggccggc gcgcgaggtg atgactccgc gcaccgaggt ggcctggatc
    17761 gacgcccggg cgggacccga ggagatccgc gaggccctgc tccagactcc ccacagccgg
    17821 atcccggtcg cggatggctc ggtcgaccag ctgatcggga tcgtccaggc gcgcgacatc
    17881 gtcgcggcgc tgatcgaggg ccggccgctc gacctgcgcg cgctcagccg ggaagcgccg
    17941 gtggtcccgg accagatgga cgcgatggac gcgctcgcgg tgctgcgcgg cagccagacg
    18001 cggatggcgt tcgtccacga cgaatatggc catttcgaag gaatcgtcac ctcggccgac
    18061 ctgctcaacg cgatttcggg cgtgctcgcc ggcggcttcg agggcgggcc cgaggcgcct
    18121 ctggtcgagc gcgaggacgg cagctggtgg gtgtcgggct cgctgtcggc cgacgccttg
    18181 tcggaccggc tcggcatcgg cctgcccgag gaccgcgact atgcgacgac ggccggcttc
    18241 gccttgtcgg tgctgaagca cattccggag gtgggcgagc gcttcacctt cggcggctgg
    18301 cagttcgaga tcgtcgacat ggacggcttc aagatcgaca agctgctcgc cagcgaggtg
    18361 gtgcgcaaga gacggggcgg ctgatgtgaa ttcgtcaccc cggacttgat ccggggtcca
    18421 ccttcttcgc tgcgttcccg ggagaaaagc aaggtggatg ccggatcaag tccggcatga
    18481 cggccctggt gcggccgagc cttacgtctg gccgccgccc agcttgccga acttggcctc
    18541 gaagccgtcg gtgtcgccgc gctgcaggta gcaggcctgc tcgaccagcc ggtcgcgggc
    18601 gattcggccc tcgaaggcgc ccatgcgccg gtcgagaagg gcgacctcgt tctcggtcag
    18661 gccggcgagc tggtcgaagc gggtgatccc gaacgcattg agctgcgcgg cgagcttcgg
    18721 gccgacgccc ttcaggaggc gcagatcgtc cggcgggccg ctcgggtccg ggatcctggg
    18781 gtgcgcgtcg acgcccagta tctcgccggc gacgtcctgg gtggcggcgg cgaactcgtc
    18841 gggcagggca ttgccctccg ggccgtcggc cgctccggag ccgcggcccc ggaaggccca
    18901 gagggcgacg gcgagtccga tcgccagcgc gacgacgagg aggatgagat t
//
LOCUS       DASVQZ010000380         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_657801, whole genome shotgun sequence.
ACCESSION   DASVQZ010000380 DASVQZ010000000
VERSION     DASVQZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657801"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..800
                     /locus_tag="VF547_10785"
     CDS             84..800
                     /locus_tag="VF547_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8263347.1"
                     /translation="MYAMSHKILLVDDDAHIRQLLAFALGKAGMKTSEAGDGEAALRE
                     VERDRPDLVVLDINMPRMDGLEVCRRLRAEGDLPILFLSSRDDEIDRVLGLEMGGDDY
                     VVKPFSPREVVARVTAILKRGGRALAEAGPKSALVHNRLRLDPEAWEAHWDGEKVPVT
                     VTEFQLLMLLASLPSKVFSRDSIIDRLHGPGFAITDRTIDSHIRNLRAKFAALGAADL
                     IETRPGIGYRIGRCVGQSAS"
     gene            797..2356
                     /locus_tag="VF547_10790"
     CDS             797..2356
                     /locus_tag="VF547_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8263348.1"
                     /translation="MIRSLKEFAKRHWPALRLRTLLFLTLLFVAALPGIGAVFLRVYE
                     NTLVQQTEAELIAQGAALAGAYRAAWPEPYADPPAGPLRPERPTIDLRAMPVLPEQPE
                     ARPSRWPPDPRAVRAAAALRPVVIDTVRSTLAAVRLMDVHGTVLLGRGDVGRSYLHLP
                     EVVPATIGQTRTVLRRRGDYRPRYALEWLSRASNIRVHYARPIVAGGEVVGVLMLSRS
                     PRGLFLGIYQDRGKIALGVGIIFLTLLVLAGLLSRGISRPIEALSEATQNVARGPVAV
                     PEPPPTAAVEIRTLYLNFAAMADRIERRSRYLKDFAAAVSHELKTPIAGIKGALELLA
                     EHPEMSEEERRRFLANAGADADRLSHLLRRLLDLARADMATAPEDAAGEAAGPALRVA
                     DAHRGEGFAVDVRLEGLPKVAAPDELLEAVLETLVENSRQSGAGRVQIEGRAQSGKVQ
                     LTVRDDGPGVPPADHERIFEPFQTGRRSEGGSGLGLSIARSLLASCAGTIVSKPAVAG
                     AEFEIVLPAAR"
     gene            2468..2836
                     /locus_tag="VF547_10795"
     CDS             2468..2836
                     /locus_tag="VF547_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263349.1"
                     /translation="MGSSAAERLQLVADLYRAVAPSLRADIAAAQDADVAHALRVNLQ
                     SLEMAFIRAGRMNLGAEGEKAEVAYAAARAAAGAAERSFAASVAASAKIAALDQAVGA
                     IDHLLRVANDDDGFGGPRFE"
     gene            2833..3288
                     /locus_tag="VF547_10800"
     CDS             2833..3288
                     /locus_tag="VF547_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263350.1"
                     /translation="MKPPPSKDSLSPQERRERYRENQNYFSEKLSEVTRYVGFGLVAF
                     AFSLLSDEMAFAKKLSDAAVSLVAIAGLCGCLVVLSDFLHLMLGWKSNELAAGNKQGD
                     YAMPPAGKRLRAAQTMFFYLKQGLAAAGSLLLIGALASAISIGPLLPDG"
     gene            complement(3305..>4534)
                     /locus_tag="VF547_10805"
     CDS             complement(3305..>4534)
                     /locus_tag="VF547_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8263351.1"
                     /translation="TEEVTGLDLVELMIRVAAGEALPFGQDDVKLNGWAIENRVYAED
                     PYRGFLPSTGRLVRYRRPRETPREVLRYAGSTGSPATQDEREKSSQPLILSSGLSEGE
                     GRVSKDHTETGVTRVDDGVEEGGEVSMFYDPMIAKLITWAPTREAAIDRQIEALDSFV
                     LEGIGHNVDFLSALMQHPRFREGRLTTGFIAEEYPEGFAGAPADERLIEDLAAIAALL
                     ALETDARACAVSGQLAAPVAPPEERVVRLGGRAFSIRFDAFEGGLLAYLDDGEGRELI
                     GRWEPGQRLFKGTIDGRERTVQVARSGRQWRLTTRGASHVVEVLPSHVAELSIHMIEK
                     VPPDLSKYLLCPMPGLLTALNVGEGDRVEAGQPLAVVEAMKMENILRAEKAGSVAAVR
                     AKPGDSLAVDAVILEFE"
BASE COUNT          690 a         1648 c         1508 g          688 t
ORIGIN      
        1 ctgcgccgct ccaccgccga ccgccgcctc tgcgacggca accccttccc cgctttgaca
       61 gcgacgcccg cgaggtgaaa gacatgtacg cgatgagcca caagatcctc ctcgtcgacg
      121 acgacgccca catccgccag ctgctcgcct tcgcgctcgg caaggcgggg atgaagacga
      181 gcgaggccgg cgacggcgag gcggcgctga gggaggtcga gcgcgaccgg cccgacctcg
      241 tcgtcctcga catcaacatg cctcggatgg acgggctcga agtctgccgg cggctgcggg
      301 cggaaggcga tctgccgatc ctcttcctct cctccagaga cgacgagatc gatcgggtcc
      361 tcgggctcga gatgggcggc gacgactatg tcgtgaagcc cttttctccg cgcgaggtcg
      421 tcgcgagggt caccgcgatc ctcaagcgcg gcggtcgggc gctggccgag gcggggccga
      481 agtcggcgct ggtccacaac cggcttcggc tcgatcccga agcctgggag gcgcattggg
      541 acggcgagaa ggtcccggtc acggtgaccg agttccagct gctgatgctg ctcgccagcc
      601 ttccctccaa ggtgttcagc cgggactcga tcatcgaccg gctgcacggc cccggcttcg
      661 cgatcaccga ccgcacgatc gacagccaca tccgcaacct gcgcgccaag ttcgccgctc
      721 tcggcgcggc cgacctgatc gagacgcggc ccggcatcgg ctaccggatc ggccgctgcg
      781 tcggccagag cgcttcttga tccgaagcct caaggaattc gccaagcgcc actggccggc
      841 gctcaggctt cgcaccctcc tcttcctcac cctgcttttc gtcgccgcgc ttcccggcat
      901 cggcgccgtc ttccttcggg tctacgagaa caccctggtc cagcagaccg aggccgagct
      961 gatcgcccag ggcgcggccc tcgccggcgc ctatcgcgcc gcctggccgg aaccctatgc
     1021 ggaccctccg gccggccccc tccggcccga gcggccgacc atcgacctgc gcgccatgcc
     1081 ggtgctgccc gagcagccgg aagcccggcc ttcgcgctgg ccgccggacc cccgcgccgt
     1141 ccgcgccgcc gccgctctga ggccggtcgt gatcgacacc gtccgctcga cgctcgcggc
     1201 ggtccggctg atggacgtcc acggcacggt gctgctcggg cgcggcgacg tcggccgctc
     1261 ctatttgcac ctgcccgaag tggtcccggc gaccatcggc cagacccgta ccgtgctgcg
     1321 ccgccgcggc gactaccggc cgcgctacgc gctcgaatgg ctcagccggg cgtccaacat
     1381 ccgggtccat tatgcgcggc cgatcgtcgc cggcggcgag gtggtcggag tgctcatgct
     1441 gagccgcagc ccgcgcggcc ttttcctcgg catctaccag gaccggggca agatcgcgct
     1501 cggcgtcggg atcatcttcc tgacccttct ggtgcttgcc ggcctgctgt cccggggcat
     1561 ctcgcggccg atcgaggcgc tgagcgaggc cacccagaac gtcgcccgcg ggcccgtcgc
     1621 ggtgcccgag ccgccgccga ccgccgcggt cgagatccgc accctctatc tgaacttcgc
     1681 cgccatggcg gaccggatcg agcggcgctc gcgctacctc aaggatttcg ccgccgccgt
     1741 cagccacgag ctgaagacgc cgatcgccgg gatcaagggc gcgctcgagc tgctcgccga
     1801 gcatcccgag atgagcgagg aggagcgccg ccgcttcctc gccaatgccg gcgccgacgc
     1861 cgaccgcctc tcccacctgc tgcgccgcct gctcgacctc gcgcgcgccg acatggcgac
     1921 cgcgcccgag gacgcggcgg gcgaagcggc ggggccggcg cttcgggtcg ccgacgccca
     1981 tcgcggcgag ggcttcgcgg tggacgtccg gctcgaaggc cttcccaagg tcgcggctcc
     2041 ggacgagctg ctcgaggcgg tgctagagac tctcgtcgag aacagccgcc agtccggcgc
     2101 cggccgggtc cagatcgagg ggcgcgccca aagcggcaag gtccaactca ccgtccgcga
     2161 cgacggcccg ggggtgccgc ccgccgatca tgagcgaatc ttcgagccct tccagaccgg
     2221 ccgccgaagc gaaggcggct ccggcctcgg cctgtcgatc gcccgctcgc tcctcgcctc
     2281 ctgcgccgga accatcgtca gcaagcccgc cgtggccgga gcggaattcg agatcgtgct
     2341 gcctgcggca cgctgacgga acccgggcgc tctccgactc gactcgaaca ttcagacgac
     2401 ggataatcag gcgatcgagc tcaggggccg actaagcgcc cgccggcgac acgagaggag
     2461 gaggaaaatg ggcagttccg ccgccgagcg gctgcaactg gtcgcggacc tgtacagggc
     2521 cgttgcgccg agcttgcggg cggacatcgc cgcggcgcag gacgccgatg tggcccatgc
     2581 ccttcgggtg aacctgcaga gcctggaaat ggcgttcata cgggccggac ggatgaacct
     2641 cggcgccgag ggcgaaaagg ccgaggtcgc ctacgcagcg gcacgtgcgg ccgcgggcgc
     2701 cgctgaacgt tctttcgcgg caagcgtggc ggcaagcgcg aagatcgccg cgctggacca
     2761 ggcggtcggg gccatcgacc atctgctgcg cgtggccaat gacgacgacg gtttcggggg
     2821 accgcgtttc gaatgaagcc gccgccgtcc aaggacagcc tgagcccgca ggagcggcgc
     2881 gaaaggtaca gggagaacca gaactacttt tccgaaaagc tcagcgaggt cactcgctat
     2941 gtcggattcg gcctggtcgc cttcgctttc agcctgctgt cggatgagat ggcctttgcc
     3001 aaaaaattgt cggacgcggc ggtcagcctc gtcgcgatcg ccgggctttg cggatgcctc
     3061 gtcgtgctca gcgatttcct gcatctcatg ctcgggtgga agtccaacga attggcggcg
     3121 ggcaacaagc agggggatta tgcgatgccc ccggcaggaa agagactgcg ggcggcgcaa
     3181 acgatgtttt tctatctcaa gcaagggctg gcggccgccg ggagcctgct gctcattggc
     3241 gccctggcca gcgccatttc gatcggaccg ctgcttccgg acggctagcg cgccggggcg
     3301 ccgctcactc gaattccagg atcaccgcgt cgacggcgag gctgtcgccc ggcttggccc
     3361 tcaccgccgc gaccgatccc gctttctccg ccctcaggat attctccatc ttcatcgcct
     3421 cgacgacggc gagcggctgg cccgcctcga cccggtcgcc ctcaccgacg ttgagggcgg
     3481 tgaggaggcc gggcatcggg cagagcagat atttggaaag gtccggcggc accttttcga
     3541 tcatgtggat cgagagctcg gcgacgtgcg agggaagcac ctcgacgaca tgggacgcgc
     3601 cgcgcgtcgt cagccgccac tggcggccgc tccgcgccac ctggacggtc cgctcgcggc
     3661 cgtcgatcgt ccccttgaac agccgctggc ccggctccca ccgtccgatc agctcgcgtc
     3721 cctcgccgtc gtcgagatag gcgagcagcc cgccctcgaa ggcgtcgaag cggatcgaga
     3781 aggcccgtcc gccgaggcgc accacccgct cctcgggagg ggcgaccgga gccgccagct
     3841 ggccgctcac cgcgcaggcg cgggcatccg tctccagggc gaggagggcg gcgatcgccg
     3901 ccaggtcctc gatcaggcgc tcgtccgcag gcgcgccggc aaagccttcc ggatactcct
     3961 cggcgatgaa gccggtggtc agccgcccct cgcggaagcg cggatgctgc atcagcgcgg
     4021 agaggaagtc gacattgtgg cctatcccct ccagcacgaa cgaatcgagc gcctcgatct
     4081 ggcgatcgat cgccgcctcg cgggtcggcg cccaggtgat cagcttggcg atcatcggat
     4141 cgtagaacat cgagacttcg ccgccctcct cgaccccgtc gtcgactcgg gtgacaccgg
     4201 tctcagtgtg gtccttcgat acgcggcctt cgccttcgct cagtccgcta ctcaggatga
     4261 gcggctggga actcttttcc cgctcatcct gagtagccgg tgagcctgtc gaaccggcgt
     4321 atcgaaggac ctcccgaggg gtttcacgcg gcctccggta ccgcaccagc cgccccgtcg
     4381 agggcaggaa gccccgatag gggtcctcgg catagacgcg gttctcgatc gcccagccgt
     4441 tcagcttcac atcgtcctgc ccgaacggca gcgcctcgcc cgcggcgacc cggatcatca
     4501 gctcgaccag atcgagtccg gtcacctcct cggt
//
LOCUS       DASVQZ010000381         8262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_849143, whole genome shotgun sequence.
ACCESSION   DASVQZ010000381 DASVQZ010000000
VERSION     DASVQZ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849143"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VF547_10810"
     CDS             complement(<1..1077)
                     /locus_tag="VF547_10810"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX8263352.1"
                     /translation="MRLADLAGIGGDSRVTGFAIDHRKVAPGTVFGAFRGSVFNGEDF
                     IPQAVGAGAVAVVARPGVPVEGAVHLAAPEPRREFARLAAKFFEPFPETVVAVTGTNG
                     KTSSVELTRQLWRMAGHHAASIGTLGVTTADDRTTTGLTTPDVVTFLSNMAGLAREGV
                     THAAFEASSHGLSQYRTEGLKVSAGAFTNFTRDHLDYHETMEAYFEAKMRLFDEVVAD
                     GGAAVVWTDDPKSDEVRRRAEARGLRLLSVGGRGETLRLVGREPTQLGQRLTIEAEGE
                     THKIELPLIGAYQAANALTAAGLVLATGGELDRTLACLPRVQGVRGRLERAVITRAGA
                     PVYVDYAHTPDAIEAAIEALRPHAR"
     gene            complement(1081..2775)
                     /locus_tag="VF547_10815"
     CDS             complement(1081..2775)
                     /locus_tag="VF547_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8263353.1"
                     /translation="MATAAIYPDRFGGAGQRPQGLALTYQRLMLMMLVFAGVTLLVAG
                     RLLFLQLFTDRGGAGQIGNPLVPVRADIVDRHGVPLARTIDAWSIAVHPDRLLGDPGE
                     LAVKLAELMPERSAADYKAMLTSRKTFVYLSRRAVPELVAAVNALGEPAIEFSREPQR
                     LYPQTAMAGHILGWTDFDGRGVAGMERVLDRRLSDPAQRGTPVALSIDARVQGVMETE
                     LGAAMAKFSAIGAAGIVLDIQTGEVIAMTSLPQLNPNKPGRDAEQAGFNRATLGVYEL
                     GSTFKPFTVAMAMDAGIIKSFGQMYNCPRGLPAGGFTITDTHPFGRACSVAEIMKESS
                     NIGTAQIAAQIGSARQQEFLRKMGFLEPVSIELNERGRTLTPGANWGEVATMTVGYGH
                     GIAVTPLHLANGYATLFNGGIWRPATLVKYDSRHPVPQGRRVFTEETSHRMRALLRLV
                     VTDGTGGKADAPGYRVGGKTGTAEKIVGGRYTGSAVVTTFAGVFPMDEPRYVVVAMLD
                     DPKSTAETFGFHTAGWNVAPVVSRVIGRIGALLGVRPDTTRDVNMAEVLPYVQKEG"
     gene            complement(2775..3599)
                     /locus_tag="VF547_10820"
     CDS             complement(2775..3599)
                     /locus_tag="VF547_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263354.1"
                     /translation="MIARGFKSVIWVATVGSAALACYMVSLRVATERNELGKVERQII
                     AAKRDIRSLQTELGTRGRMTQLEQWNAEVLALSAPSSAQFLKDEFTLARLYRSDPTVA
                     DRSAEVRLASAQVGAPPAETARPAAPAVVQAVAEPAAAAPPLVHRASFAAAEPAARPK
                     PPVAKADKPALQRTAAVVPAEAAAKPATTPGKPAARRSAERTASAVPAKPEAGAGRAG
                     AGKPGSKPATSAAKTAANPAAAAARSAAKPAAVAARPLREAAAAAREGKGKGTGGR"
     gene            complement(3596..4552)
                     /gene="rsmH"
                     /locus_tag="VF547_10825"
     CDS             complement(3596..4552)
                     /gene="rsmH"
                     /locus_tag="VF547_10825"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX8263355.1"
                     /translation="MPAPHVPVLLDEVIAGLAVAPGETHVDGTFGAGGYTRAILEKGA
                     ARVYAFDRDPDAIQEGEILAASSGGRLTLVPERFSRMRQALAARDVDSVDGVTLDIGV
                     SSMQLDRADRGFSFQADGPLDMRMGREGASAADFLNHAPEEEIADVLWRFGEEPRSRR
                     IARAIVAARPLERTAQLAAIVRKATGWNPGMKKDPATRTFQAIRIHLNEELEELEGGL
                     EAAEQVLKPGGRLAVVTFHSLEDRLVKRFLKERSGDLPSGSRHLPEQPESRPAATFDG
                     VARPVRASETEIKANPRARSATLRVARRTSATPWTGREGMPS"
     gene            complement(4573..5058)
                     /locus_tag="VF547_10830"
     CDS             complement(4573..5058)
                     /locus_tag="VF547_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX8263356.1"
                     /translation="MEVDDFFQGSALNAVDVKGRLSVPAFVRSVIERRSDARAIVVGA
                     HEVAPCLTASGRNYAKNLWTDLERRRLAEEDRGGTLDDHYARARRMFGMTEDVPYDPS
                     GRIVLPPTMRRKGRIEDLALFVGIGGIVEIWNPRVALVEGDRDLKEIAAWRLEEKGIA
                     P"
     gene            complement(5307..6218)
                     /locus_tag="VF547_10835"
     CDS             complement(5307..6218)
                     /locus_tag="VF547_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263357.1"
                     /translation="MPFQRQFARSSSNGFHGPGARRNGGPRQAWPVLTAVASLLFLTA
                     DAPPDPPPDVMLMTGLPLIWGEKGAFDLSSRRPTVYYYLIHSFRFTPVDVLDAETLEG
                     GRLLFLAQPHRLAPAELAALDGWIRAGGRALILTDPMLTWPSELPPGDIRRPPPTGLL
                     APLLDHWRLTMDGPEEPSEVEAFWNGRRIVMDSPGRLRSSSPDCTVAAGGWTALCRLG
                     RGTVRVVADADLLRDSTWAPNGLDRPVADNPAVVGEWLDRLAGTPRPRPRLAKAAAGG
                     GLVAPALLLALLAAAGIGLLLRRRRTR"
     gene            complement(6237..6512)
                     /locus_tag="VF547_10840"
     CDS             complement(6237..6512)
                     /locus_tag="VF547_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263358.1"
                     /translation="METEKRVQRVRIGLTGLAFVFLLVLLGAVFTSPSNEAPITPNLI
                     ETQLSGGNQALPAANAAEPKEPLAELGVAPGNADDDNGAAPAPAPAR"
     gene            6631..7014
                     /locus_tag="VF547_10845"
     CDS             6631..7014
                     /locus_tag="VF547_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263359.1"
                     /translation="MMKSPLRLSALPLAALVALAACQNEPIDPNKEDQANAAAAANAA
                     ASVEAPPMIQASRTYRCKDNSLLYADFYTNDTVNVRAQKDGPGTTLTAPAAGQPYVAE
                     GYSLSANAAQISYTAPGKGAQSCKA"
     gene            complement(7106..7570)
                     /locus_tag="VF547_10850"
     CDS             complement(7106..7570)
                     /locus_tag="VF547_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8263360.1"
                     /translation="MKPIPEGYHSVTPYMIVDGAAEAIRWYGEAFGAIEALRLPMGDK
                     IGHAEIRIGDSVVMLADEWPDMGMLGPKARGGATMSLMIYVEDSDSVFARAIAAGATE
                     ERPVETQFYGDRSGTLIDPFGHRWTISTHVEDVPDDEIQRRMAEFARAHEPA"
     gene            7671..>8262
                     /locus_tag="VF547_10855"
     CDS             7671..>8262
                     /locus_tag="VF547_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX8263361.1"
                     /translation="MPDRITPAVVAEHGLSPEEYERVLHALGREPNLTELGIFSVMWS
                     EHCSYKSSRIHLKKLPTTAPWVICGPGENAGVIDIGDGQAAIFKMESHNHPSYIEPYQ
                     GAATGVGGILRDVFTMGARPVANLNALRFGRPDHPRMRHLIAGVVAGIGGYGNCVGVP
                     TVGGEVNFHRAYDGNILVNAMTVGIAEQDRIFYSAAA"
BASE COUNT         1219 a         2913 c         2915 g         1215 t
ORIGIN      
        1 ccgggcatgg ggccgaagag cctcgatcgc cgcctcgatc gcgtcgggcg tatgggcata
       61 atcgacatag acgggcgcgc ccgcccgggt gatcaccgcg cgctcgagcc gcccgcggac
      121 gccctgcacc cgcggcaggc aggcgagggt ccggtcgagc tcgccgccgg tggcgagcac
      181 cagcccggcc gccgtgagag cgttcgcggc ctggtaggcc ccgatcagcg gaagctcgat
      241 cttgtgcgtc tcgccctccg cctcgatggt caggcgctgg ccgagctggg tcggctcgcg
      301 gccgaccagc ctcagcgtct cgccgcggcc gccgacgctg agcaaccgca gcccgcgcgc
      361 ctcggcgcgg cggcggacct cgtccgactt tgggtcgtcc gtccacacga ccgcggcgcc
      421 gccgtccgcg acgacctcgt cgaacagccg catcttggcc tcgaaataag cctccatggt
      481 ctcgtgatag tcgagatggt cgcgggtgaa gttggtgaag gcgccggcgc tcaccttcag
      541 cccctcggtg cggtattggg aaaggccgtg gctcgaagcc tcgaacgcgg catgggtgac
      601 gccctcccgc gccagccccg ccatgttgga gaggaaggtg acgacgtccg gagtcgtcag
      661 gccggtcgtc gtccggtcgt cggcggtcgt gaccccgagc gttccgatcg acgccgcatg
      721 gtgccccgcc atccgccaca attggcgggt cagctcgacg ctcgacgtct tgccgttggt
      781 cccggtgacg gcgaccaccg tttccggaaa gggctcgaag aatttcgcgg cgagccgagc
      841 gaactcgcgt cgaggctcag gcgcggcaag gtggacggcg ccctcgaccg gaacgcccgg
      901 gcgggccacc acggcgaccg ccccggcccc gaccgcctgg gggatgaagt cctcgccgtt
      961 gaagacgctt ccgcggaagg cgccgaacac cgttccgggc gcgaccttgc ggtggtcgat
     1021 cgcgaagccc gtcacccggg aatcgccgcc gattcccgcg aggtcggcga ggcgcatcgg
     1081 tcagccttcc ttttgaacgt aggggagcac ctccgccatg ttgacgtcgc gagtcgtatc
     1141 gggccggact ccgagcaggg cgccgatccg gccgatcacc cgcgacacca ccggagcgac
     1201 gttccagccg gcggtgtgga agccgaaagt ctcggccgtg gacttgggat cgtcgagcat
     1261 ggcgacgacc acatagcggg gctcgtccat cgggaaaacg cccgcgaagg tggtgaccac
     1321 cgccgagccg gtatagcggc cgccgacgat cttctcggcg gtgccggtct tgccgccgac
     1381 gcggtagccg ggcgcgtccg ccttgccgcc ggtgccgtcg gtgacgacca gccggagcag
     1441 ggcccgcatc cggtgggacg tctcctcggt gaacacgcgg cggccctgcg gcaccggatg
     1501 gcggctgtcg tacttgacga gggtcgccgg ccgccagatg ccgccgttga acaaggtcgc
     1561 gtagccgttc gcgaggtgga gcggagtgac cgcgatgccg tggccgtagc cgaccgtcat
     1621 cgtcgcgacc tcgccccaat tggcgcccgg cgtcagcgtc cggccgcgct cgttcagctc
     1681 gatcgacacc ggctcgagga agcccatctt gcggaggaat tcctgctggc gcgcggaacc
     1741 gatctgggcc gcgatctgcg ccgtgccgat attcgaggat tccttcatga tctcggcgac
     1801 ggagcaggcg cgcccgaacg gatgggtgtc ggtgatggtg aagccgccgg ccggcaggcc
     1861 gcgcggacaa ttgtacatct gcccgaagct cttgatgatg ccggcgtcca tcgccatcgc
     1921 gaccgtgaag ggcttgaagg tcgatccgag ctcgtagacg ccgagcgtcg cccggttgaa
     1981 cccggcctgc tccgcgtccc gcccaggctt gttcgggttg agctggggca gagaggtcat
     2041 cgcgatgacc tcgccggtct ggatgtcgag gacgatgccg gcggcgccga tcgcgctgaa
     2101 cttggccatg gcggcgccga gctcggtctc catcacgccc tggacccggg cgtcgatcga
     2161 cagcgcgacc ggagtcccgc gctgggcggg gtcgctcagc cgcctgtcca gcacccgctc
     2221 catgcccgcg accccgcggc cgtcgaagtc cgtccagccc aggatgtggc cggccatggc
     2281 cgtctgcggg tagaggcgct ggggctcgcg gctgaactcg atcgccggct cgccgagcgc
     2341 gttcaccgcg gcgaccagct cggggacggc gcggcggctg agatagacga aggtcttgcg
     2401 cgacgtcagc atggccttgt agtcggccgc gctccgctcc ggcatcagct cggcgagctt
     2461 gaccgcgagc tcgccggggt cgcccagcag ccggtcggga tggaccgcga tcgaccaggc
     2521 gtcgatcgtc cgcgccaggg gcactccgtg gcggtccacg atgtcggcgc gcacgggcac
     2581 cagcgggttg ccgatctggc cggcgccgcc gcggtcggtg aacagctgaa ggaacagcag
     2641 ccggccggcg acaagcaggg tcaccccggc gaagaccagc atcatcagca tgagccgctg
     2701 ataggtcagc gccagaccct gcggccgctg gccggcgccg ccgaagcggt cgggatagat
     2761 ggcggcggtc gccatcagcg gccgcccgtc cctttgccct tgccctcgcg cgcggccgcg
     2821 gcggcctcgc gcagcggcct ggcggcgacg gccgccggct tggccgccga cctggccgcg
     2881 gcggcggccg gattcgccgc cgtcttggcc gcggacgtcg cgggcttcga gcccggcttg
     2941 ccggcgccgg cgcggccggc accggcctcc ggcttcgccg gaaccgccga ggccgttcgc
     3001 tcggcggagc gcctggcggc cggcttgccc ggcgtcgtcg cgggcttcgc ggccgcctcg
     3061 gccggcacca ccgcggcggt gcgctgcagg gccggcttgt cggccttggc gacgggcggc
     3121 ttgggcctgg cggccggttc ggccgcggcg aagctcgccc gatggaccag cgggggcgcc
     3181 gcggcggccg gctcggcgac cgcctgaacg acggccggag cggccggcct tgccgtctcc
     3241 gcgggcggcg cgccgacctg ggcggaggcg agcctcacct cggccgagcg gtccgccacg
     3301 gtcggatcgc tgcggtacag ccgggcgagg gtgaattcgt ccttgaggaa ctgggccgag
     3361 ctgggcgcgg aaagggccag cacctcggcg ttccactgct cgagctgggt catccgcccg
     3421 cgcgttccga gctcggtctg gagcgagcgt atgtcgcgct tggcggcgat gatctggcgc
     3481 tcgaccttgc cgagctcgtt gcgctcggtc gcgacgcgca gcgacaccat gtagcaggcc
     3541 agcgcggcgc tgccgacggt agcgacccag atgacggact tgaagcccct tgcgatcatg
     3601 agggcatccc ttccctgccg gtccatgggg tggccgacgt gcgccgggcg acccgcaagg
     3661 tcgccgagcg cgcgcgcgga ttggctttga tttcggtctc gctggcgcgg accggcctgg
     3721 cgacgccgtc gaacgtggcg gccggccggc tctccggctg ctcgggaagg tgcctggagc
     3781 ccgaggggag gtcgccgctg cgctccttga ggaagcgctt gaccagcctg tcctcgaggc
     3841 tgtggaaggt gacgacggcc agccggccgc cgggcttgag cacctgctcg gccgcttcga
     3901 ggccgccttc gagctcctcc agctcctcgt tgagatggat ccggatcgcc tggaaggtgc
     3961 gagtcgccgg atccttcttc atgccggggt tccagccggt cgccttgcgg acgatggccg
     4021 cgagctgggc ggtgcgctcg agcggacgtg cggcgacgat cgcgcgcgcg atgcgccgcg
     4081 acctcggctc ctcgccgaag cgccacagca cgtcggcgat ctcctcttcc ggggcatggt
     4141 tgaggaagtc cgccgcgctg gcgccctcgc gccccatgcg catgtcgagc ggcccgtcgg
     4201 cctggaagga gaagccgcga tcggcgcggt ccagctgcat cgaggagacg ccgatgtcga
     4261 gcgtgactcc gtcgaccgag tcgacgtcgc gggcggcaag cgcctgacgc atccgggaga
     4321 aacgctccgg aacaagcgtc aggcgccccc cgctcgaagc cgccagaatc tcgccttctt
     4381 gtatcgcatc gggatcgcgg tcgaaggcgt agactcgggc agcccctttc tcgagaatgg
     4441 ctctggtgta gccgccggcg ccgaaggtgc cgtcgacatg ggtctcgccg ggggcgacgg
     4501 cgaggccggc gatcacctcg tccaggagca cggggacgtg cggggcgggc acgtgcgacg
     4561 cttcccgggc actcacgggg cgatcccctt ctcctcaagg cgccaggcgg cgatctcctt
     4621 gaggtcgcgg tcgccctcga cgagcgcgac ccgggggttc cagatctcga cgatgccgcc
     4681 gatcccgacg aacagcgcaa gatcctcgat ccggcccttg cggcgcatcg tcggcggcag
     4741 cacgatccgg cccgaggggt cgtagggcac gtcctcggtc attccgaaca tccgccgggc
     4801 gcgggcataa tggtcgtcca gggtgccgcc gcgatcctcc tcggcaagcc ggcggcgctc
     4861 gaggtcggtc cacagattct tcgcgtaatt gcgtccggag gcggtcaggc agggcgcgac
     4921 ctcatgggcg ccgacgacga tggcgcgggc gtcggagcga cgctcgatga cggagcggac
     4981 gaaggcggga acggagaggc gccccttgac gtccaccgcg ttgagcgcgc tgccctggaa
     5041 gaagtcgtcg acctccaccg cccctgtccg ctcccctaca cggagcctcc ccgttcgcgg
     5101 acgcaccagg tgcgccgcgt gactcgccgg tcagccgaga aagtgcccca aaaatcagaa
     5161 ggcgtggata acatcgggcg acccccatgg gaaggggatt tcatgccttt tttggggatt
     5221 tcatgccttt aaggggcatt tcgtggtttg ttcttgacgt tgttcagctg atgttcgcgg
     5281 attccatcct gtggataagt ctgtgactag cgcgtccggc ggcggcgcag caagagtccg
     5341 attccggcgg cggcgagcag ggcgagaagc agcgccggcg cgaccagccc cccgcccgcc
     5401 gccgccttcg ccaggcgggg acgcggtcgc ggcgttccag cgagccggtc cagccactcg
     5461 ccgaccaccg ccggattgtc ggccacgggg cgatcgaggc cgttcggagc ccaggtcgag
     5521 tcgcgcagca gatcggcgtc ggcgaccacc cggacggttc cgcgcccgag cctgcacagg
     5581 gcggtccagc ctcctgcggc gacggtgcaa tcggggctcg agctgcgcag gcggccgggc
     5641 gaatccatca cgatccggcg gccgttccag aatgcctcga cctcgctcgg ctcctccggt
     5701 ccatccatcg tcagccgcca atggtccagc agcggcgcca ggagcccggt cggaggcggg
     5761 cgcctgatgt cgcccggggg aagctcgctc ggccaggtca gcatcgggtc ggtgaggatg
     5821 agcgcccgcc cgcccgcccg gatccagccg tcgagcgcgg ccaattccgc cggcgccagc
     5881 cgatgcggct gagcgaggaa cagcagccgc ccgccctcca gggtctcggc atcgagcacg
     5941 tccaccgggg tgaaccggaa ggaatggata agatagtaat agacggtggg tcgcctcgag
     6001 ctgagatcga acgccccctt ctcgccccag atcagcggca ggccggtcat cagcatgacg
     6061 tccggaggcg ggtcgggagg cgcgtcggcg gtcaggaaca ggagcgaagc gacggcggtc
     6121 aggaccggcc aggcctgtcg aggcccgccg ttcctcctgg cgccggggcc gtgaaagccg
     6181 ttcgacgagc tccgggcgaa ctggcgctgg aaaggcacct tccgatggcg ggggcgctag
     6241 cgcgcaggcg ccggcgccgg agcggcgccg ttgtcgtcgt cggcattgcc gggagcgaca
     6301 ccgagctcgg cgagcggttc cttgggctcc gcggcattgg ccgcgggaag ggcctggttg
     6361 ccgcccgaaa gctgggtctc gatcagattg ggcgtgatcg gcgcctcgtt gctcgggctg
     6421 gtgaacaccg ccccgagcag gacgagcagg aagacgaagg cgaggccggt gaggccgatg
     6481 cggacccgtt gcacgcgctt ctcagtctcc acccctggat acctccggac gctagaccag
     6541 gccgcggcct ttgtcgaacc cgcgctgctg ttgcgaagcg ctcgcagaaa agcccttgac
     6601 gacggcggct gcgctgactg aagtgaccca atgatgaaaa gccccctccg cctctccgcc
     6661 ctccccctcg ccgccctggt cgcgctcgcc gcctgccaga atgagccgat cgacccgaac
     6721 aaggaagatc aggccaacgc cgccgcggcg gccaacgccg ccgcgtcggt ggaagcgccg
     6781 ccgatgatcc aggcgagccg cacctatcgc tgcaaggaca acagcctgct ctacgccgac
     6841 ttctacacca acgacacggt caacgtccgc gcccagaagg acggcccggg cacgaccctg
     6901 accgcccccg ccgccggcca gccctatgtc gccgaaggct attcgctgag cgccaacgcc
     6961 gcccagatca gctacaccgc gcccggcaag ggcgcccaga gctgcaaggc ctaagcgcgt
     7021 cgtcccagcg aaggttggga ccgcaggggc gaagaacgcc gcctctttca cccgccggga
     7081 ccccagcctg cgctggggtg acggttcacg ccggctcgtg cgcccgggca aactcggcca
     7141 tccggcgctg gatctcgtcg tccggcacgt cctcgacatg ggtcgagatc gtccagcgat
     7201 gtccgaacgg gtcgatcagc gtgcccgacc ggtcgccgta gaactgggtc tcgaccggcc
     7261 gctcctcggt ggctccggcc gcgatcgcgc gggcgaagac cgagtccgaa tcctcgacat
     7321 agatcatcag gctcatcgtc gctccgcccc gcgccttcgg gccgagcatg cccatgtcgg
     7381 gccattcgtc ggccagcatc acgacgctgt cgccgatcct gatctccgca tggccgatct
     7441 tgtcgcccat cggaaggcga agcgcctcga tcgcgccgaa cgcctcgccg taccagcgaa
     7501 tcgcctcggc ggcgccgtcg acgatcatat agggggtaac gctatgatag ccttccggaa
     7561 ttggcttcac gcccatggcc ggctcctttc cgtctgagaa cagaagggga tcacccttac
     7621 agcggcgccg gcccttcgac aaaccatttt cgatcgaata gagcgccgcc atgcccgacc
     7681 gcatcactcc ggccgtcgtc gccgagcacg gcctctctcc cgaggaatat gagcgcgtcc
     7741 tgcacgcgct cggccgcgag ccgaacctca ccgagctcgg catcttctcg gtcatgtggt
     7801 cggagcattg ctcctacaag agctcgcgca tccacctgaa gaagctgccg acgacggcgc
     7861 cctgggtgat ctgcgggccc ggcgagaacg ccggagtgat cgacatcggc gacggccagg
     7921 ccgcgatctt caagatggag agccacaacc acccgtccta catcgagccc tatcaggggg
     7981 cggcgaccgg agtcggcgga atccttcgcg acgtcttcac catgggcgcg cgcccggtcg
     8041 ccaacctcaa cgccctgcgc ttcgggcgtc cggaccatcc caggatgcgc cacctgatcg
     8101 ccggcgtcgt cgccgggatc ggcggctacg gcaattgcgt cggagtcccg acggtcggag
     8161 gggaggtcaa tttccaccgc gcctacgacg gcaacatcct cgtcaacgcg atgacggtcg
     8221 ggatcgccga gcaggacagg atcttctatt cggcggccgc cg
//
LOCUS       DASVQZ010000382         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_467232, whole genome shotgun sequence.
ACCESSION   DASVQZ010000382 DASVQZ010000000
VERSION     DASVQZ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467232"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..560
                     /locus_tag="VF547_10860"
     CDS             45..560
                     /locus_tag="VF547_10860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8263362.1"
                     /translation="MTEREQAPAAPAEPQRPALVLGPGGLAVPVAGIKAEDLQDTYTQ
                     SRAGGARVHNAIDIMAPRGTPVIAAAPGTVEKLFFSGGGGGITAYVRSPDRQWIFYYA
                     HLDAYAPGLHEGQAVAQGDPIGTVGFTGNANPAGPHLHFAVHRMAPGEAWHGGTAVNP
                     YPLLAGPRRAR"
     gene            601..1164
                     /gene="efp"
                     /locus_tag="VF547_10865"
     CDS             601..1164
                     /gene="efp"
                     /locus_tag="VF547_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX8263363.1"
                     /translation="MKISGVDIRPGNIIEYEGGIWRAVKIQHTQPGKGGAYMQVEMKN
                     LIDGRKNNVRFRSAETVERVRLDTKDFQFLFRDGDALTFMDKDNYEQITLDAGILGDA
                     AAFLQDGMDVVMELYDERPISVQLPDTIEATIVEADAVVKGQTASSSYKPAVLDNGVR
                     VMVPPHISSGTRIVVDVYEQTYVRRAD"
     gene            1281..>1980
                     /locus_tag="VF547_10870"
     CDS             1281..>1980
                     /locus_tag="VF547_10870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8263364.1"
                     /translation="MVSHSGLITVMQKAARKAAPRLRRDFGEVEQLQVSRKGPADFVS
                     MADKLAEETVMEELRHARPDWGFLMEEAGEVQGAPGKPRWVLDPIDGTSNFLHGIPHF
                     AISIAVQESKPGGNGWGEVTQGLVYQPLTDESFWAEKGRGAWLNERRLRVSSRRHLDD
                     ALIATGVPYRGHGDLERFQKILDAVSPEVAGIRRFGSAALDLAWVAAGRYDGFWEEDL
                     QYWDVAAGILLVREA"
BASE COUNT          327 a          688 c          665 g          300 t
ORIGIN      
        1 cgctcacgag cgcattctgg ttcctggcgt tcaatcgcgg cgcactgacc gagcgcgagc
       61 aggcgcccgc ggcaccggcc gagccgcagc gcccggcgct cgtcctcgga cccggcggcc
      121 tcgccgtgcc ggtcgcgggc atcaaggccg aggacctcca ggacacctat acgcagtcgc
      181 gcgcgggcgg cgcccgtgtc cacaatgcga tcgacatcat ggcgccgcgc ggcaccccgg
      241 tgatcgcggc ggcgcccggc accgtcgaga agctcttctt cagcggcggc gggggcggga
      301 tcacggccta tgtccgctcg cccgaccggc agtggatctt ctattacgcc catctcgacg
      361 cttacgcgcc cgggctgcac gagggccagg cggtggccca aggcgatccg atcggcaccg
      421 tcggcttcac cggcaacgcc aatcccgccg gcccgcacct gcatttcgcc gtccatcgca
      481 tggcgccggg cgaggcctgg catggtggga ccgcggtcaa tccctacccg ctccttgccg
      541 ggccgcggcg cgcgcgctaa agcgccgccc gacctttgta cgtcactcag cgagaacatc
      601 atgaagatca gcggcgtgga catccgtccc ggcaacatca ttgaatatga aggcggcatc
      661 tggcgcgctg tgaagatcca gcacacccag cccggcaagg gcggggccta catgcaggtc
      721 gagatgaaga acctcatcga cggccgcaag aacaacgtcc gcttccgctc ggcggagacg
      781 gtggagcgcg tccgcctcga caccaaggat ttccagttcc tgttccgaga cggcgatgcg
      841 ctcaccttca tggacaagga caattacgag cagatcacgc tcgacgccgg catcctcggc
      901 gacgccgcgg ccttcctcca ggacggcatg gacgtcgtca tggagctcta cgacgagcgg
      961 ccgatctcgg tgcagctccc cgatacgatc gaagcgacga tcgtcgaggc tgacgccgtg
     1021 gtgaaggggc agaccgcctc gtccagctac aagccggccg tgctcgacaa cggcgtgcgc
     1081 gtcatggtcc cgccgcacat ttcgagcgga acccggatcg tcgtcgacgt ttacgagcag
     1141 acctacgtcc ggcgcgcgga ctgatctgcc aagcccctcc ccttcagggg aggggtcggg
     1201 gtggggaatg tcacagttcc tgcgccccac atcccccacc cccgtcccct cccctgaagg
     1261 ggaggggtgg agagtagacc atggtttccc attccggcct catcacggtg atgcagaagg
     1321 cggcgcgcaa ggcggcgccg cgccttcgcc gcgacttcgg cgaggtcgag cagcttcagg
     1381 tgagccgcaa gggccccgcc gacttcgtgt cgatggccga caagctggct gaagagacgg
     1441 tgatggagga gctgcgccat gcgcggcccg actggggctt cctgatggaa gaagccggcg
     1501 aggtgcaggg cgcccccggc aagccgcgct gggtgctcga tccgatcgac ggcacgtcca
     1561 acttcctcca cggcattccg catttcgcca tctcgatcgc ggtccaggag tcgaagcccg
     1621 gcggcaacgg ctggggcgag gtgacccagg gcctcgtcta ccagccgctg accgacgaga
     1681 gcttctgggc cgagaagggg aggggcgcct ggctcaacga gcggcgcctt cgcgtctcgt
     1741 cgcggcggca cctcgacgac gctctgattg cgaccggcgt cccctacagg gggcacggcg
     1801 atctcgagcg cttccagaag atcctcgacg ccgtgtcgcc ggaagtcgcc ggaatccgcc
     1861 gcttcggctc ggcggcgctc gatctcgcct gggtcgcggc cgggcggtat gacggcttct
     1921 gggaagaaga cctgcaatat tgggacgtcg cggcgggcat cttgctggtg cgcgaagccg
//
LOCUS       DASVQZ010000383         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_467273, whole genome shotgun sequence.
ACCESSION   DASVQZ010000383 DASVQZ010000000
VERSION     DASVQZ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467273"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /gene="hrpB"
                     /locus_tag="VF547_10875"
     CDS             complement(<1..1551)
                     /gene="hrpB"
                     /locus_tag="VF547_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEX8263365.1"
                     /translation="MTDLPIHAVLPELVAALRDTSNAVLVAPPGAGKTTAVAPALLAE
                     PWCGGEILLLSPRRLAARAAAERMAELAGERVGGTIGYATRLDSKRSRSTRILVLTEG
                     IFRSRIQADPELAGISAVLFDEVHERSLDSDFGLALALDAQAALRPELRLVAMSATLD
                     GERFAALMGGAPVIESPGRSFPIELRHLGRSAEKRIEDEMAAAVRRALAEAEGSLLAF
                     LPGVAEIERTAERLDPLPADVDLHRLHGSLDPAAQRAAIAAPRPGRRKLVLATSIAET
                     SLTLDGVRIVVDSGLARRPRYDRSAGITRLVTERASQAAATQRAGRSGRQGPGIVYRL
                     WAETATASLPRFDPPEIVEADLSALLLDCALWGVSDPRQLKWLDPPTAAAVDEARRRL
                     VALGALGEDGRPTAHGKAIAALPLPPRLAHMLVEAQGRGWGRTAAEVAVLLSERGLGG
                     NDVDLETRLRRWRGDKGQRAEAARGLAKRWEQLAKPSPLQGRGLGEGEPERARRRPLS
                     PTLSPEGER"
     gene            complement(1654..>1797)
                     /locus_tag="VF547_10880"
     CDS             complement(1654..>1797)
                     /locus_tag="VF547_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8263366.1"
                     /translation="RSGALADTIDRARTYGQRAIDALGAFPAGKAKSALVEAVEFAIA
                     RAY"
BASE COUNT          224 a          636 c          703 g          234 t
ORIGIN      
        1 cctctcccct tcaggggaga gggtggggga gagggggcgt cgtcgagcac gctcaggctc
       61 cccctccccc aaccctctcc cctgaagggg agagggcttg gcgagctgct cccacctttt
      121 cgccaggccc ctggccgctt ccgcgcgctg gcccttgtcc ccccgccagc gcctcagcct
      181 cgtctccagg tcgacgtcgt tgccgccgag accccgctcg gagagcagga ccgcgacctc
      241 ggccgccgtc cgcccccagc cgcggccctg cgcctcgacc agcatgtggg cgaggcgcgg
      301 cggcagcggc agcgccgcga tcgccttgcc gtgcgcggtc gggcggccgt cctcgccgag
      361 cgccccaagc gcgaccaggc ggcgccgcgc ttcgtccacc gcggccgcgg tgggcgggtc
      421 cagccacttg agctggcgcg ggtcggagac tccccacagg gcgcagtcga ggagcagcgc
      481 gctgaggtcc gcctccacga tctccggcgg atcgaaacgc ggcaggctcg cggtcgccgt
      541 ttcggcccac aggcggtaga cgatgcccgg tccctgccgt cccgagcggc cggcgcgctg
      601 ggtcgccgcc gcctggctgg cgcgctcggt gaccagccgg gtgatgccgg ccgagcggtc
      661 gtagcgggga cggcgggcga ggcccgaatc gacgacgatt cgaaccccgt cgagggtgag
      721 gctggtctct gcgatcgagg tcgccaggac gagcttgcgg cggcccggcc gaggcgcggc
      781 gatggcggcg cgctgggcgg ccgggtcgag gctgccgtgg agccggtgga ggtcgacgtc
      841 ggcgggcagc gggtcgagcc gctccgcggt ccgctcgatc tcggcgactc cggggaggaa
      901 ggcgagaagg cttccctcgg cctcggcgag cgcccggcgg acggcggcgg ccatctcgtc
      961 ctcgatccgt ttctcggcgg agcggccgag gtggcggagc tcgatcggga agctccgccc
     1021 cgggctctcg atcaccggcg cgcctcccat cagggcggcg aagcgctcgc cgtcgagggt
     1081 cgccgacatg gcgacgaggc ggagctcggg gcgcagcgcg gcctgagcgt cgagggcgag
     1141 ggcgagtccg aaatcgctgt cgaggctgcg ctcatggacc tcgtcgaaca ggaccgccga
     1201 gattccggcc agctcgggat cggcctggat ccggctcctg aagatgcctt cggtgaggac
     1261 gaggatccga gtcgagcgcg agcgcttcga gtcgagccgg gtcgcatagc cgatcgtccc
     1321 gccgacccgc tcgccggcca gctcggccat ccgctcggcg gcggcgcggg ccgcgagccg
     1381 ccggggggag aggagcagga tctcgccgcc gcaccagggc tcggcgagca gggccggggc
     1441 gacggcggtg gtcttgccgg cgccgggcgg ggcgacgagg acggcgttgg atgtatcgcg
     1501 caaagccgcg acgagctcgg ggaggacggc gtggatgggg aggtccgtca ttgcgagcgc
     1561 agcgaagcaa tccagtcggg cgtcagccgg agacaccgtt gccggattgc cgcgtcgctg
     1621 cgctcctcgc aatgacggct ttcaccttcc tcctcaataa gcccgggcga tggcgaactc
     1681 gaccgcctcg acgagcgccg acttggcctt gccggcgggg aaggcgccga gcgcgtcgat
     1741 ggcgcgctgg ccgtaggtgc gggcgcggtc gatcgtgtcg gcgagcgccc cgctccg
//
LOCUS       DASVQZ010000384         5471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_945528, whole genome shotgun sequence.
ACCESSION   DASVQZ010000384 DASVQZ010000000
VERSION     DASVQZ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945528"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VF547_10885"
     CDS             complement(<1..181)
                     /locus_tag="VF547_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263367.1"
                     /translation="MRRFAPFAAIILAACGSAPEPASTGPVLRAEAAARFSDAQAPAR
                     EAAFSPDGRLLATTSA"
     gene            complement(222..458)
                     /locus_tag="VF547_10890"
     CDS             complement(222..458)
                     /locus_tag="VF547_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263368.1"
                     /translation="MRTVSVSGDRPAGQAIGGDVAKLPASAPRGVIGTGLLFLVSLFL
                     LAAGGAPSMPAGDPAATGDVGAFLLDPGVTPAAG"
     gene            626..1435
                     /locus_tag="VF547_10895"
     CDS             626..1435
                     /locus_tag="VF547_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8263369.1"
                     /translation="MPDRTPTTEGPIRVGVGGWDYDPWRESYYPPKLSKAKQLEHMAG
                     RLTAVEINATYYKLQKPELFERWAKAVPADFRFAVKGSRFCSNRKILGEAGESVERFC
                     GQGLAELGDKLGPILWQFMATKRFEPDDFRAFLDLLPRTVAGVPLRHAVEPRHESFRD
                     PAFVEMARSAGVATVFADSDEFPCIADLSGDLVYARLQRSRAEVPTGYTLPEIERWAE
                     VAKGWARGESPAGFPYAANPPPPRPRDTYVFFISGAKERNPAAAEALIGRL"
     gene            1609..2763
                     /locus_tag="VF547_10900"
     CDS             1609..2763
                     /locus_tag="VF547_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX8263370.1"
                     /translation="MNPLFANLPTTVFTRMSALAMEHGAVNLGQGFPDFGWPDDVIAR
                     AAQALIRGSNQYAPMPGLPELRQAVARHYAAHQGIETDPATVVVTSGATEALAASLMA
                     LIEPGDEVLLFQPLYDAYLPLVLRAGGVPRFVRLTPPDWRIEEAALASAFTPGTKLVI
                     FNNPHNPSSRVFDADELGLLAEACLRHGAIVVTDEVWEHVVFDGRRHVPLASLPGMAG
                     RTVKVGSAGKIFSLTGWKVGWVVAPPELAGPISKAHQFITFATAPNLQSAVAYGLGRE
                     AGWFDAMRSGFAASRDLLVDGLREAGFATLAAEGTYFVGVDLPASGIEADDSTFCDRA
                     VREAGVAAIPLSAFYAEDPVTSVVRLCFAKKPGTLAAGVERMAKARALFD"
     gene            2830..4323
                     /locus_tag="VF547_10905"
     CDS             2830..4323
                     /locus_tag="VF547_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8263371.1"
                     /translation="MTFNSTNEARALFERLGVALPEGGLESRSPIDGLVIGSVPTASP
                     ADVAAAVGRAHEAFLAWRTVPPPRRGELVRLFGDVLRAEKQALGRLVSLEAGKILQEG
                     LGEVQEMIDICDFAVGLSRQLYGLTIATERPGHRMMETWHPLGVVGVISAFNFPVAVW
                     AWNACLAFVCGDPVVWKPSEKTPLTAVAVQALFERAAERFGESPPHLSQVVQGGRETG
                     EALVDDPRVALVSATGSTAMGRAVAPRVAARFGRTLLELGGNNGMVVAPSADLALAER
                     AIAFAAVGTAGQRCTTLRRLFVHDDVYDELVPRLKHVWATVPVGNPLEDGTLVGPLID
                     EAAVAAMERALQRARAEGGRAFGGERIEGGYVRPAIVEMPRQTAITLTETFAPILYVM
                     RYSGLDEAIAAHNEAPQGLASSIFTNDIREAETFLSAAGSDCGIVNVNIGPSGAEIGG
                     AFGGEKETGGGRESGSDAWRNYMRRATNTINYSRELPLAQGINFSMP"
     gene            4320..5177
                     /locus_tag="VF547_10910"
     CDS             4320..5177
                     /locus_tag="VF547_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263372.1"
                     /translation="MKRRVAILTPDRTSEGYRTRWPDVLARTAAPLREAGLEVEARDW
                     AAADDLASFDLVMPLLVWGYPFAPRRWAAAVDRWEGEGARLRNPASVLRWNADKAYLG
                     RLAERGAPVVPTRHYTRLNRSVLEEAASALGSDRLVAKPRVSSTAWQTIRWSPGQDLD
                     GGPEGAAIVQPYLPEIETSGEVSMIFLGGTFSHAVSKRPQPGDFRVQPEFDGIIAGHR
                     PAPEEHSAAERILAAVDEPLLYARVDLVRGLDGTAQLIELELIEPDLYLGHEEDAGRR
                     FAQAVLEAL"
     gene            5252..>5471
                     /locus_tag="VF547_10915"
     CDS             5252..>5471
                     /locus_tag="VF547_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263373.1"
                     /translation="MGHVVLLGDSILDNGAYVRGGPDVVRQLRSKLPVNWDASLLALD
                     GAVTREVRGQLARLPAGASHVVVSAGGND"
BASE COUNT          761 a         1919 c         1981 g          810 t
ORIGIN      
        1 aagcgctggt cgtcgcgagc agccggccgt cggggctgaa cgccgcctca cgcgccgggg
       61 cctgcgcgtc gctgaaccgc gccgcggcct ccgcgcgcag gaccggcccg gtcgaggccg
      121 gctcgggcgc gcttccgcag gcggcgagga tgattgcggc gaacggggcg aagcgcctca
      181 tgcgcctcgt ctagcgaatc cgtgggatgg gggcgaagcc ttcagcccgc cgcgggagtc
      241 accccgggat ccagaaggaa ggcgccgacg tccccggtcg cggcgggatc gccggccggc
      301 atggaaggcg cgccgccggc cgcgagcagg aacagggaga cgaggaaaag aagaccggtg
      361 ccgatgacgc cccggggcgc cgaagccggc agcttcgcca cgtccccccc gatagcctgg
      421 ccggccggcc ggtctcccga aacgctgact gtcctcatct ccgcgctcct gctcaccgcc
      481 tcgaagtaga gggtcgaagc ggtgcgccgc cagcatcatt acggccaagt ttcggcacag
      541 cggccgaggc gcgcggctgg ccttgacgca ggcgtgcggt tatgtgcggg attgtacgga
      601 caaacccgag gcgcgaggtt cgccgatgcc cgatcggacg ccgaccaccg agggccccat
      661 tcgcgtcgga gtcggcggct gggactacga tccctggcgg gagagctact atccgccgaa
      721 gctgtcgaag gcgaagcagc tcgagcacat ggccgggcgg ctgaccgcgg tcgagatcaa
      781 cgcgacctac tacaagctgc agaagcccga gcttttcgag cgctgggcga aagcggtgcc
      841 ggcggatttc cgcttcgccg tcaagggctc gcgcttctgc tccaaccgca agatcctcgg
      901 cgaggccggg gagtcggtgg aacggttctg cgggcagggc ctggccgagc tgggcgacaa
      961 gctcgggccg atcctgtggc agttcatggc gacgaagcgg ttcgagccgg acgatttccg
     1021 cgcattcctg gacctgctgc cgcgcacggt ggcgggcgtg ccgctgcggc acgcggtcga
     1081 gccgcgtcat gagagcttcc gcgatccggc ctttgtcgag atggcgaggt ctgccggcgt
     1141 ggcgaccgtg ttcgccgatt ccgacgaatt cccctgcatc gccgacctct cgggcgacct
     1201 cgtctatgcg cggctgcagc gcagccgtgc ggaggttccg accggctata cgctcccgga
     1261 gatcgagcgc tgggccgagg tcgcgaaggg ctgggcgcgg ggcgaaagcc ccgccggctt
     1321 cccctacgcc gccaacccgc ccccgccgcg gccgcgcgac acctacgtct tcttcatctc
     1381 cggagcgaag gagcgcaatc ccgcggcggc cgaagctctg atcggccggc tgtgacggcc
     1441 tccccatcgc cagatgggga gggcgaccgc cgaagcgggt ggagggtggc tcgggacggc
     1501 ctaagggctt ctcgtgcgac cctcccgggc tgcgagccag gtatccctcc accgccaaaa
     1561 atggcggtcc ccctccccat gctgcgcccg gggaggcata ggggacccat gaacccgctc
     1621 ttcgcgaacc ttccgaccac cgtcttcacc cgcatgtcgg cgctggcgat ggagcatggg
     1681 gcggtgaacc tgggccaggg ctttcccgat ttcggctggc cggacgacgt catcgcccgc
     1741 gccgcgcagg cgctgatccg cggctccaac caatatgcgc cgatgcccgg cctgcccgag
     1801 ctcaggcagg ccgtcgcccg ccattacgcc gcccaccagg ggatcgagac ggacccggcc
     1861 acggtcgtcg tcacgtcggg cgccacggag gcgctcgccg cctcgctgat ggcgctgatc
     1921 gagcccggcg acgaagtgct gctcttccag ccgctctacg acgcctatct gccgcttgtg
     1981 ctgcgcgccg gcggggtgcc gcgcttcgtc cggctgaccc cgccggactg gcgcatcgag
     2041 gaagcggcgc tggcttccgc tttcacgccg gggacgaagc tcgtcatctt caacaacccg
     2101 cacaatcctt cgtcgcgcgt gttcgacgcg gacgagctgg gcctgctcgc cgaggcctgt
     2161 ctgcgccatg gcgcgatcgt cgtcaccgac gaggtgtggg agcatgtcgt gttcgacggc
     2221 cgccgccatg tgccgctggc gagccttccg ggaatggccg ggcgcaccgt caaggtgggc
     2281 tcggcgggca agatcttctc gctgaccggg tggaaggtcg gctgggtggt cgccccgccg
     2341 gagctggccg gaccgatctc gaaggcgcac cagttcatca ccttcgcgac ggcgcccaac
     2401 ctgcagtcgg ccgtggccta cgggctgggc agggaggcgg gctggttcga cgcgatgcgc
     2461 agcggcttcg cggcatcccg cgatctcctc gtcgacggcc tgcgcgaggc cggcttcgcg
     2521 accctggcgg ccgagggcac ctatttcgtc ggggtcgacc tgcccgcctc ggggatcgag
     2581 gccgacgatt cgaccttctg cgaccgcgcc gtgcgcgagg cgggcgtggc ggcgatcccg
     2641 ctttcggcat tctatgccga ggatccggtg accagcgtcg tccggctgtg cttcgccaag
     2701 aagccgggga ccctggcggc cggggtcgag cggatggcga aggcgagagc gctgttcgac
     2761 tgatttgccc ccatcgggca agcgaccggc aactggcgcc ggcgctcggc catggctaaa
     2821 cgccgcgcga tgaccttcaa ttcaaccaat gaagctcgcg ccctgttcga acgcctcggc
     2881 gtcgcccttc ccgagggcgg cctcgaatcc cgctcgccga tcgacggcct cgtcatcggc
     2941 tcggtcccga ccgcctcgcc ggccgacgtc gccgccgcgg tgggccgagc ccacgaggcc
     3001 ttcctcgcct ggcggacggt gccgccgccc cgacgcggcg agctggtccg actcttcggc
     3061 gacgtgcttc gggcggagaa gcaggcgctc gggcggctgg tgagcctcga ggcggggaag
     3121 atcctccagg aagggctcgg cgaggtccag gagatgatcg acatttgcga cttcgccgtc
     3181 ggcctgtcgc gccagctcta tggcctcacc atcgccaccg agcggccagg gcatcggatg
     3241 atggagacct ggcacccgct cggagtcgtc ggagtgatct cggcgttcaa cttcccggtc
     3301 gcggtctggg cctggaacgc ctgcctcgcc ttcgtctgcg gcgatccggt ggtgtggaag
     3361 ccctcggaga agaccccact gacggcggtc gcggtgcagg cgctgttcga gcgggcggcg
     3421 gagcggttcg gtgagtcgcc gccgcatctt agccaggtcg tgcagggcgg gcgcgagacc
     3481 ggcgaggcgc tggtcgacga tccgcgggtg gcgctggtct cggcgaccgg ctcgacggcg
     3541 atgggccgcg cggtggcgcc gcgggtcgcc gcgcgcttcg gccgcaccct gctcgagctc
     3601 ggcggcaata acggcatggt cgttgcgccc agcgccgatc tcgcgctcgc cgagcgggcg
     3661 atcgccttcg ccgccgtcgg cacggccggc cagcgctgca cgaccttgcg gcgcctgttc
     3721 gtccacgacg atgtctacga cgagctcgtg ccgaggctga agcacgtctg ggcgacggtc
     3781 ccggtcggca acccgctcga ggacggcacc ctggtcggcc ccctgatcga cgaggcggcg
     3841 gtggcggcga tggagcgggc gctgcagcgg gcgcgggccg aaggcggccg cgcgttcggc
     3901 ggcgagcgga tcgagggcgg ctacgtccgg ccggcgatcg tcgagatgcc ccgccagacc
     3961 gcgatcacgc tcaccgagac gtttgcgccg atcctctacg tcatgcgcta ttcggggctg
     4021 gacgaggcga tcgcggcgca caacgaagcg ccgcagggcc tggcctcctc catcttcacc
     4081 aacgacattc gcgaggcgga gaccttcctc tccgcggcgg gctccgactg cggcatcgtc
     4141 aacgtcaaca tcggcccgtc gggcgccgag atcggcggag cgttcggcgg cgagaaggag
     4201 accggcggcg gccgcgagag cggctccgac gcctggcgca actacatgcg gcgggcgacc
     4261 aacacgatca actacagccg cgagctgccg ctggcgcagg gcatcaactt ctcgatgcca
     4321 tgaagcggcg ggtcgcgatc ctgactccgg accggaccag cgaagggtac cggacgcgct
     4381 ggccagacgt gctggcccgc accgccgcgc cgctgcgcga ggccgggctc gaggtcgagg
     4441 cgcgcgactg ggccgccgcc gatgatctcg cttccttcga cctggtcatg ccgctgctcg
     4501 tctggggcta tccgttcgcg ccgcgccgct gggccgcggc ggtggatcgc tgggaggggg
     4561 agggggcccg gctgcggaac ccggcgtcgg tgctgcgctg gaacgccgac aaggcctatc
     4621 tcgggcggct tgcggagcgc ggcgcgccgg tcgttcccac ccgccattac acccggctga
     4681 atcggtccgt cctggaagag gcggcgagcg ccctgggcag cgaccggctg gtcgccaagc
     4741 cgcgggtctc ctcgaccgcc tggcagacga tccgatggtc gccggggcag gatctcgacg
     4801 gcgggccgga aggggcggcg atcgtccagc cctatctgcc ggagatcgag acgtcgggcg
     4861 aggtctcaat gatcttcctc ggcggcacgt tcagccacgc cgtctcgaag cggccgcagc
     4921 ccggcgattt ccgggtccag cccgagttcg acgggatcat cgccgggcac cggccggccc
     4981 cggaggagca ttcggcggcg gagcggatcc tcgccgcggt cgacgagccg ctgctctacg
     5041 cccgggtcga cctggtccga ggcctcgacg gtaccgcgca gctgatcgag ctggagctga
     5101 tcgagccgga cctttacctg ggtcatgaag aggatgcggg gcgccgtttc gcgcaggcgg
     5161 tgctggaggc gctctaattc ctcccggaga aatctcgggg aggaatttcg acgccggaac
     5221 cgatcggcgc ctgagccgct acttcccccc catgggccat gtcgttcttc tcggcgattc
     5281 gatcctcgac aacggcgcct atgtgcgcgg cgggccggac gtcgtccgcc agctgcgctc
     5341 gaagcttccg gtcaattggg acgccagcct gctcgccctc gacggcgcgg tgacccgcga
     5401 ggtccgcggc cagctggctc ggctccccgc cggcgccagc catgtcgtcg tcagcgccgg
     5461 cggcaacgac g
//
LOCUS       DASVQZ010000385         5461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_849450, whole genome shotgun sequence.
ACCESSION   DASVQZ010000385 DASVQZ010000000
VERSION     DASVQZ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849450"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VF547_10920"
     CDS             <1..341
                     /locus_tag="VF547_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX8263374.1"
                     /translation="AQIIDVRIKRADLPSGTPLDSAYERMRTARQQEAGAIRAQGYKQ
                     AQIIRADADAEAASTYAEAYGKDPDFYAFYRAMQSYETTFAPGENQGRSSIVMSPDNQ
                     YLQEFKGKNR"
     gene            540..2087
                     /locus_tag="VF547_10925"
     CDS             540..2087
                     /locus_tag="VF547_10925"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX8263375.1"
                     /translation="MRYVYGLSAAILLGGAAATLTVGPVGAQTAQNEPGAMSAAAPRA
                     GAPMSFADLAARLQPAVVNISTKQVLQVNRSRQVPPGFEDFFRQFGVPVPDGGDAPIT
                     QRGGSLGSGFIISADGYVVTNNHVVSPARSGATVEQITVTLPDRREFEATLVGRDSAS
                     DLALLKIKGNDLPFVRFGDSQQVRVGDWVVAIGNPFGLGGTVTAGIVSALHRNLNAGV
                     YDRYIQTDASINMGNSGGPMFDLNGNVIGINTALISPTGGNVGIGFAIPAEQARPVID
                     ALRRGERPQRGYIGVSLQTLDEDIAASLGIPKNQGELIRGVTAGGPAARAGIQQGDVV
                     VSVGGRPVTPDESLAYLVSRQPVGARVPIELVRDGRRQTVTVTVGARPTEEELARLNG
                     IEDETPVTEPAESQQSTSQRSARESLGVTVQTLTPEIARSLRLSDVNVRGVVIASVNP
                     SSDAAAKGIQPGDIILSINQRATRTPEEAAAAVAAARAAGRNSVLLLIRRGNAPPAYY
                     GIALAGR"
     gene            2166..3785
                     /locus_tag="VF547_10930"
     CDS             2166..3785
                     /locus_tag="VF547_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX8263376.1"
                     /translation="MDDDGSIFIGLGGGGPQRLVLRRANRHGLIAGATGTGKTVTLQG
                     LAEGFSQVGVPVFLADVKGDLGGLAMAGSPLGKLHDAFAERAAEIGDAGWSYGDNPVQ
                     FWDLLGRQGHPIRTTVSEMGPLMLARLMDLNEVQEGVLTIVFHVADERGLLLLDLDDL
                     QAMLADCAARADELTTTYGNVSKASVGTIQRSLLQLRSQGGDSFFGEPALEIEDLLAT
                     DKRGRGVINILAADKLMASPKLYSTFLLWLLSELFEQLPEIGDPEKPRLVFFFDEAHL
                     LFASAPAALLEKIEQVVRLIRSKGVGVYFITQNPVDIPDAVAGQLGNRIQHALRAFTP
                     RDEKAVRSAAETFRANPGLDVATAITELKVGEALVSLLQPDGSPSPVERTLIRVPRSR
                     VGPITPDERALLVQTDSVGAKYDEPIDRESAQELLAAKAGEAKAAAAEAEALEEQARL
                     DAAEAKQAERERLLAEREAARAAKEQERARIAAEREAARRAREAARPSLTDKLVESAA
                     RSAASSIGRQVAGRAGGQLLRGLLGGLFRGR"
     gene            3921..>5461
                     /locus_tag="VF547_10935"
     CDS             3921..>5461
                     /locus_tag="VF547_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX8263377.1"
                     /translation="MAAEGKMRELTVRSIVLGGILTLVFTAANVYLGLKVGLTFATSI
                     PAAVISMAVLRWFASSTILENNIVQTVASAAGTLAAIIFVLPGLVMIGWWQDFPFVTT
                     AAITMLGGILGVLFSVPLRRALVVDSPLPFPEGRAAAEVLRVGSRSQEGAEESRRGLS
                     VLVWTGLASAGFAILTQTRLAAAEAAAWFRAGAGATGISGGLSFALMGVGHLVGISVG
                     LAMFLGLVIGWWILLPILTAQNPLPGGAEAWARAVFTQDVRFFGAGVIGVAAVWTLLR
                     IAGPVIGGIRSAMAASAARRGGAALALEERDLPIAIVGFGSLAMLVPIAWLLWSVIDG
                     GALAGSAALLIAGALLFVIVIGLMIAAVCGYMAGLIGASNSPVSGIGILSVVAASLML
                     VGLFGRDTPPGTSEALVAYGLIVTGIVFGVATISNDNLQDLKTGQLVGATPWRQQVAL
                     IFGVIFGSLVVPPVLQLLNAGIGFAGMPGAGPNALPAPQAALISALAKGVLGGDLNWT
                     MIGYGA"
BASE COUNT          786 a         1966 c         1896 g          813 t
ORIGIN      
        1 gggcgcagat catcgacgtc aggatcaagc gtgccgatct gccgagcggg acgccgctcg
       61 actcggccta tgagcggatg cgcaccgccc gccagcagga ggccggggcg attcgggcgc
      121 agggctacaa gcaggcccag atcatccgcg ccgacgcgga cgccgaagcg gccagcacct
      181 acgccgaagc ctatggcaag gaccccgatt tctacgcctt ctaccgggcg atgcagagct
      241 acgagacgac cttcgcgccg ggcgagaacc agggccggtc gagcatcgtc atgtcgccgg
      301 acaaccaata tctgcaggag ttcaagggca agaatcgata ggaagagccg tcattgcgag
      361 cgaagcgaag caatccagct ccgcgcgagc ggggagcacc gcctctggat tgccgcgtcg
      421 cttcgctcct cgcaatgacg ggccgctatt caaatccggt tcagccacac caattacgtt
      481 tgtaatcgca acacccggat atgcccagcc gggccgaagc aagtgaggag atacgaatcg
      541 tgcgttacgt ctatggcctc tccgccgcca ttctcctggg cggcgccgcc gcgacgctga
      601 cggtgggccc cgtcggcgcc cagaccgcgc agaacgagcc cggagccatg tccgccgcgg
      661 cgccgcgcgc cggagcgccg atgagcttcg ccgatctcgc cgcccggctc cagccggcgg
      721 tcgtcaacat ctcgaccaag caggtgctgc aggtgaaccg cagccgccag gtcccccccg
      781 gcttcgagga tttcttccgc cagttcgggg tgcccgtccc ggacggcggc gatgccccga
      841 taacccagcg cggcggctcg ctcggttcgg gcttcatcat ctccgccgac ggctatgtcg
      901 tcaccaacaa ccacgtcgtg tccccggcgc gaagcggcgc cacggtcgag cagatcaccg
      961 tgaccttgcc ggaccggcgc gagttcgagg cgaccctggt cggccgcgac agcgcctccg
     1021 acctcgccct gctcaagatc aagggcaacg acctgccgtt cgtccgcttc ggcgattcgc
     1081 agcaggtcag ggtcggcgac tgggtggtgg cgatcggcaa tccgttcggc ctcggcggca
     1141 ccgtcaccgc cggcatcgtc tccgcgctcc accgcaacct caacgccggc gtctacgacc
     1201 gctacatcca gaccgacgcc tcgatcaaca tgggcaattc gggcggtccg atgttcgacc
     1261 tgaacggcaa cgtgatcggc atcaacaccg cgctcatctc gccgaccggc ggcaatgtcg
     1321 gcatcggctt cgccatcccc gccgagcagg cccggccggt gatcgacgcc ctgcggcggg
     1381 gcgagcgccc ccagcgcggc tatatcggag tcagcctgca gacgctcgac gaggacatcg
     1441 cggcctcgct cggaattccg aagaaccagg gcgagctgat ccgcggggtg accgcgggcg
     1501 gcccggcggc gcgcgccggc atccagcagg gcgacgtcgt cgtcagcgtc ggcggccgcc
     1561 cggtcacgcc ggacgagtcg ctcgcctatc tcgtctcgcg tcagccggtc ggagcgcggg
     1621 tgccgatcga gctcgtccgc gacggccgcc ggcagaccgt caccgtcacc gtcggcgccc
     1681 gtcccaccga ggaggagctg gcgcggctca acggcatcga ggacgagact ccggtcaccg
     1741 agccggccga gtcccagcag tcgaccagcc agcgctccgc gcgggagagc ctcggggtca
     1801 ccgtgcagac gctgactccg gagatcgcgc gttcgctccg gctgagcgac gtcaacgtca
     1861 ggggcgtcgt gatcgcgtcg gtcaatccga gcagcgacgc cgccgccaag ggcatccagc
     1921 cgggcgacat catcctgtcg atcaaccagc gcgccacccg caccccggag gaggccgccg
     1981 cggcggtcgc cgccgcgcga gccgccggcc gcaacagcgt cctcctgctg atccgccgcg
     2041 gcaacgcgcc gccggcctat tacggaatcg cgctcgccgg acgctgaccg gtcgggctcc
     2101 tcggcccctt gccgcccttc ccctgttcgg ggatagggcg gcctgcggct gcagggagcg
     2161 aagttgtgga cgacgacggc tctatcttca tcgggctggg cggcggcggg ccgcagcggc
     2221 tggttctgcg ccgcgccaat aggcatggcc tgatcgccgg cgccaccggc accggcaaga
     2281 ccgtcaccct gcagggcctg gccgaaggct tctcccaggt cggggttccg gtcttcctcg
     2341 ccgacgtcaa gggcgacctc ggcggcctgg cgatggccgg atcgccgctg ggcaagctcc
     2401 acgacgcgtt cgccgagcga gccgccgaga tcggcgacgc cggctggagc tacggcgaca
     2461 atccggtcca gttctgggac ctgctcgggc gccagggcca tccgatccgc accaccgtct
     2521 ccgaaatggg ccccctgatg ctggcccggc tgatggatct caacgaggtc caggagggcg
     2581 tgctcaccat cgtcttccac gtcgccgacg agcgggggct gctgctcctc gatctcgacg
     2641 acctgcaggc gatgctcgcc gactgcgcgg cgcgagcgga cgagctcacg acgacctacg
     2701 gcaacgtctc caaggcgagc gtcgggacga tccagcgctc gctcctccag ctccgctcgc
     2761 agggcggcga cagcttcttc ggcgagccgg cgctcgagat cgaggatctc ctcgccaccg
     2821 acaagcgggg gcggggggtg atcaacatcc tcgccgccga caagctgatg gcgtcgccca
     2881 agctctattc gaccttcctc ctctggctcc tgtccgagct gttcgagcag ctccccgaga
     2941 tcggcgatcc ggagaagccc aggctggtct tcttcttcga cgaggcgcac ctgctcttcg
     3001 ccagcgctcc ggcggccctg ctcgagaaga tcgagcaggt cgtccggctg atccgctcca
     3061 agggcgtcgg ggtctatttc atcacccaga atccggtcga cataccggac gccgtcgccg
     3121 gccagctcgg caatcgaatc cagcacgcgc tgcgcgcctt caccccccgc gacgagaagg
     3181 cggtccgctc ggccgccgag acgttccggg ccaatccggg gctggacgtg gcgaccgcga
     3241 tcacggagct caaggtcggc gaggctttgg tgtcgctgct ccagcccgac ggctcgccgt
     3301 cgcccgtcga gcggacgctg atccgggtac cgcgctcccg cgtcgggccg atcacgccgg
     3361 acgagcgcgc cctgctcgtc cagaccgact cggtgggcgc caaatatgac gagccgatcg
     3421 atcgcgaatc ggcgcaggag ctgctcgccg ccaaggcggg cgaggccaag gccgccgccg
     3481 ccgaggcgga ggcgctcgag gagcaggccc ggctcgacgc cgccgaggcg aagcaggccg
     3541 aaagggaaag gctgctcgcc gagcgcgagg cggctcgagc ggccaaggaa caggagcgcg
     3601 cccgaatcgc ggccgagcgc gaggcggcgc gccgggcgcg ggaggcggcg aggccgagcc
     3661 tgacggacaa gctggtcgaa tcggccgccc gctccgcggc gagctcgatc ggccggcagg
     3721 tcgccggccg ggccgggggc cagctgctgc ggggattgct cggaggcctg ttccgggggc
     3781 ggtgattccc gcgttgcccg cgaggatcgg cggaccgaca ggacacccgt tcgcgacgcc
     3841 ggggcaggcg ctcgtcgcag gccccttgcc gccgcccgcc gcgcccgcga agaggagtgc
     3901 ggcggcaggg agtgaggcgt atggcggctg aaggcaagat gcgggagctc accgtccgct
     3961 cgatcgtgct gggcgggatc ctcaccctcg tcttcaccgc ggcgaacgtc tatctcggcc
     4021 tcaaggtcgg gctgaccttc gcgacgtcga tcccggcggc cgtcatctcg atggcggtgc
     4081 tgcgctggtt cgcgtcctcg accatcctcg agaacaacat cgtccagacc gtcgcctccg
     4141 ccgccgggac gctggcggcg atcatcttcg tcctgcccgg gctggtgatg atcggctggt
     4201 ggcaagactt cccgttcgtg acgacggcgg cgatcaccat gctcggcggc atattggggg
     4261 tcctgttctc ggtgccgctg aggcgcgccc tggtcgtcga cagccccttg cccttcccgg
     4321 aaggccgggc ggcggccgag gtgcttcgag tcggctcccg gtcccaggag ggggccgagg
     4381 agagccgtcg cggcctgtcg gtgctggtct ggaccggact cgcctcggcc ggcttcgcga
     4441 tcctcaccca gacccggctg gccgccgccg aggccgccgc ctggttccgc gccggcgccg
     4501 gcgcgaccgg aatctcgggc ggcctctcct tcgccctgat gggggtcggc catctggtcg
     4561 ggatctcggt cggcctggcg atgttcctcg ggctggtgat cggttggtgg atcctgctgc
     4621 cgatcctgac cgcccagaac ccgctcccgg gcggcgccga agcctgggcc cgcgccgtct
     4681 tcacccagga cgtgcgcttc ttcggcgccg gagtgatcgg ggtcgccgcg gtgtggaccc
     4741 tgctcaggat cgccggaccg gtgatcggcg gaatccgctc ggcgatggcg gcgagcgcgg
     4801 cccggcgcgg cggcgccgcg ctggcgctcg aggaacggga tctcccgatc gcgatcgtcg
     4861 gcttcggctc gctcgcgatg ctggttccga tcgcctggct gctgtggagc gtgatcgacg
     4921 gcggggccct ggccggctcg gcggcgctgc tgatcgccgg cgccttgctg ttcgtgatcg
     4981 tcatcgggct gatgatcgcg gccgtctgcg gctacatggc gggcctgatc ggggcgtcga
     5041 acagccccgt ctcggggatc ggaatcctct cggtcgtcgc cgcctcgctg atgcttgtcg
     5101 ggctgttcgg ccgcgacacg ccgccgggca cctccgaggc cctcgtcgcc tatggtctga
     5161 tcgtcaccgg catcgtcttc ggagtcgcga ccatctccaa cgacaacctc caggatctca
     5221 agaccggcca gctggtcggg gcgaccccgt ggcgccagca ggtcgcgctg atcttcggag
     5281 tgatcttcgg ctcgctggtc gttccgccgg tgctgcaact gctcaatgcc ggcatcggat
     5341 tcgccgggat gcccggcgcc gggccgaacg cactgccggc gccccaggcc gccctgatct
     5401 cggcgctcgc aaagggggtg ctcggcggcg acctcaactg gacgatgatc ggctacgggg
     5461 c
//
LOCUS       DASVQZ010000386         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_658823, whole genome shotgun sequence.
ACCESSION   DASVQZ010000386 DASVQZ010000000
VERSION     DASVQZ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658823"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2483
                     /locus_tag="VF547_10940"
     CDS             <1..2483
                     /locus_tag="VF547_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX8263378.1"
                     /translation="FPIIIAIYYAGELVWRERDRRMHEIIDATPLPNWAYVVPKTMAV
                     TLVLFATLLFAVVAAVLVQLIRGYTDIRLDQYFLWFVLPQGVSMVMIAVLAVFVQSIS
                     PNKYIGWAVMVLYIVSTIVMNSLGFEHNLYQFAGTPQEPLSDMNGAGRYWIGAWWFRL
                     YWAAFCVILLVVSHLLWRRGTETRLKPRLRRAPRRLAGTPGIVAGAALVVFVATGAWA
                     YYNTNVLNDFETSSAAEQFMAEYERRYLKHENVLQPTISKVALDVALYPDEARALVKG
                     RYVLTNLTPLPVQMVHVRLTDRNLDLLRLDFPGAELSQNDEEFGYRIYRLAKPMEPGE
                     SRTLSFETRRWQRGFRNSGNDTRLVGNGTFLNNMEIAPAIGMNRLGLLQERAKRRKYG
                     LPPELRPARLEDLSATGRSYFGGGWSIADITVSTVADQTPIAPGKKVSDEVRGGRRTA
                     RFLSDAPILTFFSIQSAHYAEKHRNHRGVDLAVYYHPGHERNVDRMLDALAGAMDYYQ
                     ANFGPYQFDQARIIEFPGYATFAQAFANTMPYSEAIGFVADNSDPDKIDYVTYVTAHE
                     LGHQYWAHQIIGADMQGGTMLSETLAQYSALMVMKHRYGEDKIRRFLKFELDGYLRRR
                     GGELVEEQPLMRVENQQYIHYNKGSLVMYLLQDRLGEAAVNRALRTILDKYKFKGAPY
                     PRSIELVEAFRREARTPEDRALITDLFERITLYDLKVAEPRAVQRPDGKWDVTVPVEA
                     TKFYADGKGNEKPAAFADRIAIGLFTAEPGRGAFDRRNVLTMQSQRVQSGKQVFRFVT
                     ARKPTHAGVDPYNFYIDRNSDDNVGPVA"
     gene            2616..3155
                     /locus_tag="VF547_10945"
     CDS             2616..3155
                     /locus_tag="VF547_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263379.1"
                     /translation="MPKLKQIAIDVDVNRAIENQRRSFSESENDILRRILLPITPETR
                     PPRPKATAPDTASAIVTRSRGQWTVEILGERRPAANLQDAYRRLLLHFHARRPDFLQK
                     FSLEKGRSRRFVARKPSDLYLKSPHLASDHAKELTPGWYFDTNLSMEQVASRARVAAR
                     LCGLHYGTDVRILENLREI"
     gene            complement(3181..>4179)
                     /locus_tag="VF547_10950"
     CDS             complement(3181..>4179)
                     /locus_tag="VF547_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8263380.1"
                     /translation="GSLPGGRDGRLVVVSNDLAWSADATHIAPTLQAALDDWDRLSPQ
                     LELLATDLEHGVIPRERFHEREALAPLPRAYQWADGSAYVNHVALVRQARAAEMPESF
                     WTDPLMYQGGSDEMLAPRSPIPLADEAWGCDCEAEVVVVTGDVPRGVTPEEARERIRL
                     VGLVNDVSLRNLIPAELGKGFGFLQSKPASALSPVLVTPAQLGERWRDGKLHGALKVD
                     LNGRPFGRADAGVDMTFDFGTLIAHLAKTRNIGAGSIIGSGTVSNRGEDGGPGKPIEQ
                     GGLGYSCIAEVRTVETILEGAAKTGFLKRGDTVRIWMDDEAGHPIFGVIEQTVTGS"
BASE COUNT          729 a         1416 c         1340 g          694 t
ORIGIN      
        1 tgttcccgat catcatcgcc atctattatg cgggcgagct ggtctggcgc gagcgcgacc
       61 gccggatgca cgagatcatc gatgcgactc cgctccccaa ctgggcctat gtggtgccca
      121 agacgatggc cgtcaccctc gtcctgttcg cgacgctgct gttcgcggtg gtcgccgcgg
      181 tgctggtcca gctgatccgg ggctataccg acatccggct cgaccaatat ttcctctggt
      241 tcgtcctgcc ccagggcgtc agcatggtga tgatcgcggt gctcgccgtc ttcgtccagt
      301 cgatcagccc caacaaatat atcggctggg ccgtgatggt cctctacatc gtctcgacga
      361 tcgtgatgaa ttcgctcggg ttcgagcaca atctctacca gttcgccggc acgccgcagg
      421 aacccttgtc ggacatgaac ggcgcgggcc gctactggat cggcgcctgg tggttccggc
      481 tctactgggc cgccttctgc gtcatcctgc tcgtcgtctc gcacttgctg tggcggcgcg
      541 gcaccgagac gaggctgaag ccgcgcctgc gccgggcgcc gcggcggctg gccggcacgc
      601 ccgggatcgt cgccggcgca gcgctcgtcg tcttcgtcgc taccggcgcc tgggcctatt
      661 acaacaccaa cgtcctcaac gatttcgaga cgagcagcgc ggccgagcaa ttcatggccg
      721 aatacgagcg gcgctacctc aagcatgaga acgtcctcca gcccacgatc agcaaggtcg
      781 ccctggacgt cgctctctat ccggacgagg cccgggcgct ggtcaagggc cgctacgtcc
      841 tcaccaacct cacgccgctg ccggtccaga tggtccatgt ccggctcacc gaccgcaatt
      901 tggacctgct ccggctcgac ttccccggcg cggagctcag ccagaatgac gaggagttcg
      961 gctacaggat ctaccggctc gcgaagccga tggagcccgg cgaaagccgg actttgagct
     1021 tcgagacccg gcgctggcag cgcggcttcc gcaactccgg caacgacacc aggctggtcg
     1081 ggaacggcac cttcctaaac aatatggaga tcgctccggc gatcgggatg aaccggctgg
     1141 ggcttctcca ggagcgggcg aagcggcgca aatacggtct tccgccggag ctgcgccccg
     1201 ccaggctcga ggacctgtcg gcgaccgggc ggagctattt cggcggcggc tggtcgatcg
     1261 ccgacatcac cgtctcgacc gtcgccgacc agaccccgat cgcgcccggc aagaaggtgt
     1321 ccgacgaggt ccgcggcggc cgccgcaccg cgcgcttcct ctccgacgct ccgatcctca
     1381 ccttcttctc gatccagtcg gcgcattacg ccgagaagca ccgcaaccat cgcggcgtcg
     1441 acctggccgt ctattaccac ccgggccacg agcggaacgt cgaccggatg ctcgacgctc
     1501 tggccggggc gatggactat taccaggcca atttcggccc ctatcagttc gaccaggcgc
     1561 ggatcatcga gttccccggc tatgcgacct tcgcccaggc cttcgccaac accatgccct
     1621 attcggaggc gatcggcttc gtcgcggaca acagcgatcc cgacaagatc gactacgtca
     1681 cctacgtcac cgcccacgag ctcggccacc aatattgggc gcaccagatc atcggggcgg
     1741 acatgcaggg cgggaccatg ctgtcggaga cgctcgccca atattcggcg ctgatggtga
     1801 tgaagcaccg ctacggcgag gacaagatcc gccgcttcct caagttcgag ctcgacggct
     1861 atctcaggcg ccgcggcggc gagctggtcg aggagcagcc gctgatgcgg gtcgagaacc
     1921 agcaatatat ccactacaac aagggctcgc tggtcatgta cctgctgcag gaccggctcg
     1981 gcgaggccgc ggtcaaccgg gcgcttcgca ccatcctcga caagtacaag ttcaagggcg
     2041 ccccctatcc gcgctcgatc gagctggtcg aggcgttccg ccgggaggcg aggacgcccg
     2101 aggaccgggc gctgatcacc gacctgttcg agcgcatcac cctctacgac ctcaaggtgg
     2161 cggagccgag ggcggtgcag cggccggacg gcaaatggga cgtgaccgtc cccgtggaag
     2221 cgaccaaatt ctacgccgac ggcaagggca acgaaaagcc cgcggccttc gccgaccgga
     2281 tcgcgatcgg cctgttcacc gccgagcccg gccgaggcgc cttcgaccga aggaacgtgc
     2341 tgacgatgca gtcccagcgg gtgcaaagcg ggaagcaggt cttccgcttc gtgacggcgc
     2401 ggaagccgac tcacgccggc gtcgacccct acaatttcta catcgaccgc aattccgacg
     2461 acaatgtcgg gcccgtcgcc tgagattagg gaccaccgac gttggcgagg aaccgcctcg
     2521 gttagagggc cgcgcctgcc aatcaaggaa ggacagccca tcttgaccgg aggcttcctc
     2581 ccttataagg gactatcctt ataagggagg ctggagtgcc caagctcaag cagatcgcga
     2641 tcgacgtcga tgtcaatcgg gcgattgaga atcagcgtcg atccttttcc gagagcgaga
     2701 acgatatcct tcgcaggata ttgctgccca tcacaccgga gacgcgtcct cctcgcccca
     2761 aggcgacagc gcccgacacg gcttccgcca tagtcacgcg gagccgcggc cagtggacgg
     2821 tcgaaatctt gggagagcga cgcccagccg cgaacctgca ggatgcgtat cggcgcctcc
     2881 tcctacattt tcatgccagg cgccctgatt tcttgcagaa attctcgctc gaaaagggga
     2941 ggtcgcggcg cttcgtagct cgcaagccat ccgatctcta tttgaaatcg ccacacctcg
     3001 cttcggacca cgcgaaggaa cttactccgg gctggtattt cgacacgaac ctttcgatgg
     3061 aacaggttgc cagtcgcgct cgcgtcgccg cgcgactttg cggcctgcac tacggaaccg
     3121 acgttcgcat tctcgagaac ctaagagaaa tctgagtccc atccgctgcc ggagctgcgc
     3181 ctaactgcct gtcaccgtct gctcgatgac gccgaagatc gggtggccgg cctcgtcgtc
     3241 catccagatc cgaacggtgt cgccgcgctt caggaagccg gtcttcgcgg cgccctccag
     3301 gatcgtctcc accgtacgca cctccgcgat gcacgaatag ccgaggccgc cctgctcgat
     3361 cggcttgccc gggccgccgt cctcgccgcg gttggagacg gtgcccgagc cgatgatgga
     3421 gccggcgccg atgttccggg tcttggcgag gtgcgcgatc agggtcccga agtcgaaggt
     3481 catgtcgacc cccgcatcgg cccggccgaa cggccggccg ttcagatcga ccttgagcgc
     3541 gccgtggagc ttgccgtccc gccagcgctc gccgagctgc gcgggcgtga cgaggaccgg
     3601 cgagagggcc gaggcgggct tggactggag gaagccgaag cccttgccga gctcggcggg
     3661 gatgaggttg cggagcgaga cgtcgttgac gaggccgacg aggcggatgc gctcgcgcgc
     3721 ctcctcgggc gtcactccgc gcggcacatc gccggtcacg accaccacct cggcctcgca
     3781 gtcgcagccc caggcctcgt cggcgagggg gatgggcgag cggggggcga gcatctcgtc
     3841 ggagccgccc tggtacatga gggggtcggt ccagaagctt tccggcatct cggcggcgcg
     3901 cgcctgccgg acgagggcga catggttcac ataggccgag ccgtccgccc attgataggc
     3961 gcgcggcagc ggcgccagcg cctcgcgctc gtgaaagcgc tcgcgcggga tgactccatg
     4021 ctcgaggtcg gtggcgagca attcgagctg ggggctgagc cggtcccaat cgtcgagagc
     4081 cgcctggagg gtcggggcga tgtgcgtcgc gtcggcggac caggcgaggt cgttgctgac
     4141 cacgacgaga cggccgtcgc ggccgccggg aagggatcc
//
LOCUS       DASVQZ010000387         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_850186, whole genome shotgun sequence.
ACCESSION   DASVQZ010000387 DASVQZ010000000
VERSION     DASVQZ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850186"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="VF547_10955"
     CDS             <1..1758
                     /locus_tag="VF547_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8263381.1"
                     /translation="TPVDVGGVKVSEGDAFDWKLVNIQDVHLSEAEGEPDRPGNDRGT
                     LLTFTIVALLILGIASVNFVNLATARASQRAREVALRKVLGANRRQLIVQFLAESILL
                     AAAAMLLALALAELTLPFLSKFLGVQLEMNYFGEGGIAVPVVAALLAVGLAGGLYPAF
                     YLSRYQPAAVLKANKSSAEPLGSGRLRAVLVVAQFAVSIGLIICTAVVYAQTEFARTT
                     DPGYRRDGLIQISNLNRAQVAPIVDTLIREMEKVEGVKGAAGTSITPASGTTLNTSIQ
                     VPGRAKPITIGWYSVHPNFFDTMGIRMLAGRKLSRQYAADSGAIPDQPKEAAQAAMRM
                     LAQRGQNVVVNELAARQMGFATPQQALGKQVKANQLPDEYGMLPATIVGVAQDSRFRS
                     LREPVEAMIYQDSGVYRSLVVRYESADPEGVRRNLERVWKRLAPEVPFEGEFSDDRLG
                     ELYSTDAARAKTFGGFALLAVLIACLGLFGLSAFTAERRTKEIGIRKVFGARSRDIVR
                     LLAWQFSKPVIVANLIAWPVAWWLMRDWLNGFDASIDLGPGPFVLAGALALAIALGTI
                     AGHALKVARANPIHALRYE"
BASE COUNT          305 a          706 c          622 g          306 t
ORIGIN      
        1 accccggtcg acgtcggcgg agtcaaggtc agcgaaggcg atgcgttcga ctggaagctg
       61 gtcaacatcc aggacgtcca cctgagcgag gccgagggcg agcccgaccg gcccggcaac
      121 gaccgcggca cgctgctcac cttcaccatc gtcgccctgc tcatcctcgg catcgcttcg
      181 gtgaacttcg tcaacctggc gacggcgcgt gcgagccagc gcgcccgcga ggtcgccctg
      241 aggaaggtgc tcggagcgaa ccgccgccag ctcatcgtcc agttcctcgc cgaatccatc
      301 ctgctcgcgg cggcggcgat gcttctggcc ctggcattgg ccgagctgac gctgcccttc
      361 ctttccaaat tcctcggcgt ccagcttgag atgaactatt tcggcgaggg cgggatcgcc
      421 gtgccggtcg tcgcggcgct gctcgccgtc ggcctcgccg gggggctcta tcccgccttc
      481 tacctgtcgc gctaccagcc cgcggcggtg ctcaaggcga acaagtcgtc ggccgagccg
      541 ctcggcagcg gccgtcttcg cgccgtgctg gtggtggcgc agttcgccgt ctcgatcggc
      601 ctcatcatct gcacggccgt cgtctacgcc cagaccgagt tcgcccggac gaccgacccc
      661 ggctaccgcc gcgacggcct catccagatc agcaacctca accgcgccca ggtcgctccg
      721 atcgtcgaca ccctgatccg cgagatggag aaggtcgagg gggtcaaggg cgccgccggg
      781 acctcgatca cgccggccag cgggaccacg ctcaacacct cgatccaggt gccgggccgc
      841 gccaagccga tcaccatcgg ctggtacagc gtccatccca atttcttcga cacgatgggc
      901 atcaggatgc tcgccgggcg caagctgtcg aggcaatatg cggccgacag cggcgccatt
      961 cccgaccagc ccaaggaagc ggcccaggcg gcgatgcgga tgctcgccca gcgcggccag
     1021 aacgtcgtcg tcaacgagct ggccgcgagg cagatgggct tcgcgacgcc gcagcaggcg
     1081 ctcggcaagc aggtcaaggc caaccagctg cccgacgaat atggcatgct gccggcgacg
     1141 atcgtcgggg tcgcccagga ttcgcgcttc cgctcgctgc gcgagccggt cgaggcgatg
     1201 atctaccagg acagcggagt ctaccggagc ctcgtcgtcc gctacgaatc cgccgacccg
     1261 gaaggcgtcc gccgcaacct cgagcgggtc tggaagcgcc tcgcgccgga ggtcccgttc
     1321 gaaggggaat tctccgacga ccggctgggc gagctctact cgaccgacgc ggcgcgggcg
     1381 aagacgttcg gcggcttcgc gctgctcgcg gtgctcatcg cctgcctcgg cctgttcggc
     1441 ctctccgcct tcacggccga gcggcgcacc aaggagatcg gcatccgcaa ggtgttcggc
     1501 gcccgcagcc gcgacatcgt ccggctgctc gcctggcaat tctcgaaacc cgtgatcgtc
     1561 gccaacctga tcgcctggcc ggtcgcctgg tggctgatgc gcgactggct gaacgggttc
     1621 gacgccagca tcgacctcgg cccgggcccg ttcgtgctcg ccggcgccct cgccctcgcc
     1681 atcgcgctcg gaacgatcgc cggccacgcg ctcaaggtcg cccgggccaa ccccattcac
     1741 gctctccgct acgagtagga cccgcgagat gtggaagaat tacctgaccg tcggcatccg
     1801 ggcgctggcc aagaaccgca cctacgcctt catcaacatc ttcgggctcg ccgtcggcct
     1861 cgccgcctgc ctgatgcttc tggtctatgt gagctacgag cgcagctacg acgagtggct
     1921 gccgaacagc gagaatgtg
//
LOCUS       DASVQZ010000388         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_756409, whole genome shotgun sequence.
ACCESSION   DASVQZ010000388 DASVQZ010000000
VERSION     DASVQZ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756409"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VF547_10960"
     CDS             complement(<1..1192)
                     /locus_tag="VF547_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8263382.1"
                     /translation="MMGFVQSAYVIARRDFVATVWSRTFLFFLLGPLLIIGMSFLFGS
                     MSERMARQDVHARVAVVAAEADFRFLADARERLVPAFGERGLPDLLREEPDYDQALQV
                     RNILGNRGKRVLAVLTGGLERPTLTGAISDDGGVSRQIGLILDEARQQRALAAAGRAP
                     PPAVLRVVAVDRSEGALAATRAITARAGQLILFMLTVLLAGMLLSNLLEEKSNKIIEV
                     LAAALPIDSIFIGKLASMLAVSLAGIAVWVGGALLGLAVWPNQGGGLPAPAVGWPLFI
                     LLVLVYYSANYLLLGALFLGIGSQASSVREVQTLSMPVTVGQMLIFLFASFAVGAFNS
                     PVGILAALFPFSSPLTMVARAAQTPELWPHALALAWQVLWVWLTVALGATLFRRNVLK
                     SGGGS"
     gene            complement(1189..>2056)
                     /locus_tag="VF547_10965"
     CDS             complement(1189..>2056)
                     /locus_tag="VF547_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8263383.1"
                     /translation="IAVPEGSIYGILGPNGAGKTTTLRMLLGIIDPDRGSRTLLGHER
                     PLEASKIVGYLPEERGLYPAMVTRDAIAFMCNLRGLPLDVGRKRAEVLMRQQGLGDYV
                     AKPVKSLSKGMAQTIQLFGTIVHRPKLIVLDEPFSGLDAINQGRLEHLIREEAAAGAT
                     IIFSTHVIAHAERLCERIAIIAQGQVRFEGRVSDARDRLRPHVRLRTRRADGPWRGAL
                     PDGAHSEDGLWSFELPREGIEPLLKALVDGGAGIEELSIERPGLHDAFVAIAGEQAAR
                     ELAESAAEGEAR"
BASE COUNT          349 a          704 c          729 g          274 t
ORIGIN      
        1 ccgaccctcc gccggacttg agcacgttgc gccggaacag cgtcgcgccc agcgccaccg
       61 tcagccacac ccacaggacc tgccaggcca gggccagggc gtgcggccac agctccgggg
      121 tctgggcggc gcgggcgacc atggtgaggg gggagctgaa cgggaagagc gcggcgagga
      181 ttccgacggg gctgttgaag gcgccgacgg cgaacgaggc gaacaggaag atcagcatct
      241 ggccgaccgt gaccggcatc gacagggtct gcacctcccg caccgagctg gcctggctgc
      301 cgatgcccag gaacagcgct ccgaggagga gatagttggc gctgtaatag accaggacga
      361 ggaggatgaa gagcggccag ccgaccgcgg gcgcgggcag gcccccgccc tggttgggcc
      421 agacggcgag gccgagcagc gcacccccca cccacaccgc gatcccggcc agcgagacgg
      481 cgagcatcga ggccagcttg ccgatgaaga tcgagtcgat cggaagcgcg gcggccagaa
      541 cctcgatgat cttgttcgat ttctcctcga gcaggttgga aagcagcatg ccggcgagaa
      601 gcaccgtcag catgaacagg atcagctggc cggcgcgcgc ggtgatcgcg cgggtcgcgg
      661 ccagagcgcc ttcggaccgg tcgaccgcga ccacgcgcag gacggccggc ggcggagccc
      721 gccccgccgc ggcgagcgcc cgctgctggc gcgcctcgtc gaggatcagg ccgatctgtc
      781 ggctgactcc gccgtcgtcg ctgatcgccc cggtaagggt cggcctttcg aggccgccgg
      841 tgagcacggc gaggacgcgc ttgccgcggt tgccgaggat attgcggacc tgcagggcct
      901 gatcgtaatc gggctcctcg cggaggagat cgggcaggcc ccgctcgccg aaggccggga
      961 ccagccgctc ccgcgcgtcc gcgaggaatc tgaaatcggc ttcggccgcg acgaccgcga
     1021 cccgcgcgtg cacgtcctga cgggccatac gctcgctcat gctgccgaac aggaacgaca
     1081 tgccgatgat cagcagcggt cccagcagga agaacaggaa ggtccgcgac cagaccgtcg
     1141 cgacgaaatc gcgccgcgcg atgacgtagg cggactggac gaagcccatc atcgggcctc
     1201 tccttccgcg gcgctctcgg cgagctcgcg cgccgcctgc tcgccggcga tggcgacgaa
     1261 ggcgtcgtgg aggcccggcc gctcgatcga caattcctcg atgccggcgc cgccgtcgac
     1321 cagcgccttg aggagcggct cgatgccctc ccgcggcagc tcgaacgacc agaggccgtc
     1381 ctcgctgtgc gcgccgtcgg gaagcgcccc ccgccacgga ccgtcggctc ggcgggtgcg
     1441 aagccggacg tgcggacgaa ggcggtcgcg cgcgtccgag acccggccct cgaagcggac
     1501 ctggccctgg gcgatgatcg cgatccgctc gcacagccgc tcggcatggg cgatgacgtg
     1561 ggtcgagaag atgatcgtcg cccccgccgc ggcctcctcg cggatcagat gctcgagccg
     1621 gccctggttg atcgcgtcga ggcccgagaa aggctcgtcg aggacgatca gcttcggcct
     1681 gtggacgatg gtgccgaaca gctggatcgt ctgcgccatg cccttggaga ggctcttcac
     1741 gggcttggcg acatagtccc caagcccctg ctggcgcatc agcacctcgg cgcgcttgcg
     1801 tccgacatcg agcggcaggc cccgcaagtt acacatgaag gcgatcgcat cgcgcgtaac
     1861 catcgccgga tagaggccgc gctcctctgg cagatagcca acgatcttgg atgcttcgag
     1921 cggccgctca tggccgagca gggtgcggct cccccgatcg ggatcgataa ttccgaggag
     1981 catccgaagc gtggtcgtct tgccggctcc attgggtccc agaatgccgt agatcgagcc
     2041 ttcgggcacg gcgatg
//
LOCUS       DASVQZ010000389         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_560907, whole genome shotgun sequence.
ACCESSION   DASVQZ010000389 DASVQZ010000000
VERSION     DASVQZ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560907"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            527..745
                     /gene="infA"
                     /locus_tag="VF547_10970"
     CDS             527..745
                     /gene="infA"
                     /locus_tag="VF547_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX8263384.1"
                     /translation="MAKEELLEMRGQVVELLPNAMFRVRLENDHEILGHTAGKMRKNR
                     IRVLVGDEVLVELTPYDLTKGRITYRFK"
     gene            762..1328
                     /locus_tag="VF547_10975"
     CDS             762..1328
                     /locus_tag="VF547_10975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX8263385.1"
                     /translation="MRLVLASQSPRRLELLKRIGVVPDAILPADIDETPLSGELPSPY
                     VQRIAAGKAAAVQAPGALVLAADTTVAAGRRILAKPEDEAEARAMLRLLSGRRHKVLT
                     AVTLVDAAGKARHRLSTSTVAFKRLAPDEIDAYIAAGEWEGKAGAYAIQGRAEALVRS
                     MSGSFSGVMGLPLYETRALLRAAGYPIG"
     gene            1321..2301
                     /locus_tag="VF547_10980"
     CDS             1321..2301
                     /locus_tag="VF547_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEX8263386.1"
                     /translation="MAEWLYEAGIGENRAILVEGGEILEAAIELPGSVRTGAILEARL
                     ARIHVPGRRGLAALAGGGEALVEPLPGGLTEGQPIRVEIVREAIPEAGRPKIAKARIS
                     DEAPRPGPSLHERIGCPAPLHGTARDLFEDAGWSELLEQAATGEIAFPGGALRMSVTP
                     AMTLFDVDGALPPTELAVAGAAGAARAIRRLGIGGSIGIDLPTVAGKAGRQAPAAALD
                     ALLPQPFERTGVNGFGFLQVVRRRERPSLPELVQGDPAGAAARALLRRAERAQGAGER
                     TIRAAPPVVALLERRPEWLDELARRIGAPVRLQADAGLSISGGHVQSAHF"
     gene            2279..2458
                     /gene="yacG"
                     /locus_tag="VF547_10985"
     CDS             2279..2458
                     /gene="yacG"
                     /locus_tag="VF547_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX8263387.1"
                     /translation="MSRAPTSSRRGGGCPVCGKPASPERTPFCSQGCRDRDLLQWLGE
                     GYRVPGPPAGLDSEQ"
     gene            2500..2575
                     /locus_tag="VF547_10990"
     tRNA            2500..2575
                     /locus_tag="VF547_10990"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2533..2535,aa:Phe,seq:gaa)
BASE COUNT          535 a         1342 c         1256 g          499 t
ORIGIN      
        1 ggatggggat aacggccagc acaagcagcg cggtcgatat gagcaagcca acgagcttcc
       61 accacatgtt cgatcctcaa ctgcccgcgg caggtatgac agggggaatg tccgctttcc
      121 acccctgcag aggccggacg cgtatggcac tcccaggatt tggatgaggc agaggcgaca
      181 gcggagcggt ttgccacgca ggtccggcgg gcagtgtctt ttccggcctg aaatcgctcc
      241 gcgaactgct gtgtacccgg ttcgttcttt ccgctttcct acctgcctcg cccgtgccat
      301 gctccgcatc ggcctgattc ggcctcgttc tttgaaccgt tcgctgcccg ttccgacatg
      361 ccgtccgctc ggaagcccat ggtgagtgcg aactttcgga ccattcggat tgtgcgggaa
      421 cgtgaacatc gtgaacttcg cacgccgctt cccgcgcccg gcccgccttt ccttttgcgc
      481 ggcccgtcgc taaggggagc caaacttgca aacgatcagg atttgaatgg ccaaggaaga
      541 acttctcgag atgcgcgggc aggtggttga gctgctgccc aacgcgatgt tccgcgtccg
      601 cctcgaaaac gatcatgaga tcctcggcca caccgccggc aagatgcgca agaaccgcat
      661 ccgcgtgctc gtcggcgacg aagtgctcgt cgagctgacg ccttacgatt tgaccaaggg
      721 tcggatcacc tatcgcttca agtaacggcg ggcgcgcgcc gatgcggctc gtcctcgctt
      781 cgcagagccc gcgccggctc gagctcctga agcggatcgg ggtcgtcccg gacgccatcc
      841 tccccgccga tatcgacgag acgccgctct cgggcgagct tccgtcgccc tacgtgcagc
      901 gcatcgccgc cggcaaggcg gcggccgtgc aggcgcccgg cgcgctggtg ctcgccgccg
      961 acacgacggt cgccgcgggg cgccgcatcc tcgccaagcc cgaagacgaa gcggaggcgc
     1021 gggcgatgct ccggctcctc tccgggcgcc ggcacaaggt gctgaccgcc gtcacgctcg
     1081 tcgacgccgc gggcaaggcg cgccaccgcc tctccacctc caccgtcgcc ttcaagcgcc
     1141 tcgcccccga cgagatcgac gcctacatcg cggccggcga atgggagggg aaggccggcg
     1201 cctacgccat ccagggccgc gcggaagcgc tcgtcaggag catgtccggg agcttctccg
     1261 gcgtgatggg actgccgctc tacgagaccc gcgcgctgct ccgcgccgcc ggatacccca
     1321 ttggctgagt ggctctacga ggcggggatc ggcgagaacc gcgccatcct cgtcgagggc
     1381 ggcgagatat tggaagcggc catcgagcta ccgggcagcg tgcgcaccgg cgcgattctc
     1441 gaggcacggc tcgccaggat ccacgtaccc ggccggcgcg ggctcgcggc gctggccggc
     1501 ggcggggaag cgctggtcga gccgcttccc ggcggactca ccgaagggca gccgatccgc
     1561 gtcgagatcg tccgcgaggc gatccccgag gcgggccggc ccaaaatcgc caaggcgcgg
     1621 atcagcgacg aggcgccgcg cccggggccg agcctgcacg agcggatcgg ctgccctgcg
     1681 ccgctgcatg gcacggcgcg ggacctgttc gaagacgccg gctggtcgga gctgctcgaa
     1741 caggccgcga ccggcgagat cgcgttcccc ggcggcgcgc tgaggatgag cgtcacgccc
     1801 gccatgaccc tgttcgacgt cgacggcgcg ctgccgccga cggagctcgc cgtcgccggc
     1861 gccgccggcg cggcccgcgc catccgccgc ctcggcatcg gcggctcgat cgggatcgac
     1921 cttccgacgg tggccggaaa ggccgggagg caggcgcccg ccgccgcgct cgacgcgctc
     1981 ctcccgcagc cgttcgagcg gaccggcgtc aacggcttcg gcttcctcca ggtcgtccgc
     2041 cgccgcgagc gcccttcttt gcccgagctg gtccagggcg atcccgccgg cgccgcggcc
     2101 cgcgccctcc tgcgccgcgc cgagcgggcg cagggtgccg gcgagcggac gatccgcgcc
     2161 gccccgccgg tcgtcgccct gctggagcgg cgcccggagt ggctcgatga actcgcccgc
     2221 cggatcggcg cgcccgtgcg gttgcaggcg gatgccggcc tctccatatc cggcggccat
     2281 gtccagagcg cccacttcta gccgccgcgg cggcggctgc cccgtctgcg gcaagcccgc
     2341 ctcgcccgag cgaacgccct tctgctcgca gggctgccgc gaccgcgacc tcctccagtg
     2401 gctcggcgaa ggctatcgtg tccccggccc gcccgcgggg ctggacagcg agcaataagc
     2461 gcccctatag gcacccgact ttccgctccg cgcggtcagg cccaggtagc tcagttggta
     2521 gagcacgtga ctgaaaatca cggtgtcggc ggttcgattc cgtccctggg caccattccc
     2581 ctcgcctgaa acaagtcccg gcgcgccacg ccgaagccgc gacgcgcgat cgtcatggcc
     2641 gcgacgaggc ccatgccgaa cagcgcgcct tcgaccgcgc cggctacggc ctggctgatc
     2701 ggcccgaacc cgctttcccc gaacaggctg ccgaactggt ccaggcggac gcgcgagccg
     2761 gggaagccct gggcgaggag atcgaggctg ccgcccatgg ttcgcccgcc gagcagcggg
     2821 atgaggaggc cgccggcggc tcccagggct ccggcgacgg caaggctgtg ctgcagcgga
     2881 cgcgtggagc gggcggcgag ccaggcggcg agaccgacga aggcgccgag gagcgcgccc
     2941 tccggcgccc cggtaatgtc gccgggcgag cgcccgacga ggaggttgaa cgcgtcgagg
     3001 ccgagcagct tggcgaaggc gcccacgatc agcccgcccg ccgccccgcc gacgacgctc
     3061 cacgggcggc gggtgccggc gaaccccgcg gcggcgatcc cgaaggccac gccgccgccc
     3121 gcgatcagcc cggccacgat gcctacgcag accagcacga ggagcaccga gacggcgccc
     3181 atccccggat cgagcggctg cgaggcgccg ccgaagccgt agagcaggcc gaccacggcg
     3241 ccggccgcgc cgccgccgat cgtccccgcg cctcccagga ctgcaaagtc tcgccagctg
     3301 taacgggcct gcgccgtccc ggcggcgtcg gggatagtcg tcgcagccgt ccgtgcccgc
     3361 atcggctcgg cagcgtcggc ggcgtgaacg gcggcgatga agcggtagcc gtgcttcggc
     3421 accgtctcga tgaacctcgg gtttgacgcc gtgtcgccga gctggcggcg gagcgtcttg
     3481 atgcactggg tgagcgcttc gtcggtcacc ggcacgccgc gccagacctc gtccatgaag
     3541 cggtccttgg agaccagccg gccatgctcg cgcaccagca aagcgagcgc atcgagatag
     3601 cgtgcgttga tttccacgct gtcaccgccg cg
//
LOCUS       DASVQZ010000390         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_756544, whole genome shotgun sequence.
ACCESSION   DASVQZ010000390 DASVQZ010000000
VERSION     DASVQZ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756544"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1857)
                     /locus_tag="VF547_10995"
     CDS             complement(<1..>1857)
                     /locus_tag="VF547_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX8263388.1"
                     /translation="VSGEVIAVATTRDPQGNEARAVTSVYLAGDDDWWFGGDNGDRMD
                     IIADSNSYRSGDTAHVQVRMPFRKATALVTVEREGVLSSFVTELSGKEPVIDVKLPGA
                     YAPDVYISVMAVRGRVAKWRVWMAEFAQRWKIPVLGRIANPTALVDLAKPSYRIGITR
                     VKVGWEAHQLKVAVRPDKERYHVRDHVRTAVQVLGPNGRPAQSAEIAFAAVDQALLQL
                     SPNESWDVLKAMMGERPLSVVTSTAAMQVVGKRHYGRKAVPAGGGGGGDLTDLVRTDF
                     KPVLLWRGRVKLDSAGRAELDVPLADSLSAYKLVAVATAGGDLFGTGSATIRTVQDLT
                     LYSGLPPLVRSGDEYSATFTLRNGTDRPMQVTASAELEPGPAPGGPQTMTVPAGGAVP
                     VTWRVTAPENVETLRWTVSARSADGRSDRVQVEQLVAPAVPVETWAATFLRIGDSPFP
                     VAPPPAALPGRGSIDVALSDSPSPSLAGVRAYMRAYPYGCFEQRLSKAVALDDRQAWS
                     AMLDELPAYVDRNGLLRYWPGEYMPGSIALTAYALSITPEAGFDWPEARKERLIGALK
                     AVVDGRLVDDNEGPADMRLLRLSALAALARNGAATPAMLDQAAIPVGDMPTAM"
BASE COUNT          261 a          667 c          669 g          260 t
ORIGIN      
        1 catcgccgtc ggcatgtcgc cgaccggtat ggccgcctgg tcgagcatcg ccggagtggc
       61 cgcgccgttg cgggccagcg cggccagcgc cgagaggcgc agcaggcgca tgtccgccgg
      121 cccctcgttg tcgtcgacga ggcggccgtc gaccaccgcc ttcagcgccc cgatcagcct
      181 ctccttgcgc gcctcgggcc agtcgaagcc cgcttcgggc gtgatcgaca gcgcataggc
      241 ggtgagcgcg atcgagccgg gcatgtactc gcccggccag tagcgcagca gcccgttgcg
      301 gtcgacatag gcgggcagct cgtcgagcat ggccgaccag gcctggcggt cgtcgagcgc
      361 caccgccttg gacagcctct gctcgaagca gccgtaagga taggcgcgca tgtaggcgcg
      421 cacgccggcc agcgacggcg agggcgaatc gctcagcgcg acgtcgatgc ttccgcggcc
      481 cggaagtgcg gccgggggcg gcgcgaccgg gaagggcgag tcgccgattc gaaggaaggt
      541 cgccgcccag gtttccaccg gcacggcggg cgcgaccagc tgctcgacct ggacccggtc
      601 cgagcggccg tccgcggagc gggccgaaac cgtccagcgc agagtctcga cattctccgg
      661 cgcggtcacg cgccaggtga ccgggacggc gccgcccgcc ggaacggtca tcgtctgcgg
      721 cccgccgggc gccgggccgg gctcgagctc cgcgctggcg gtcacctgca tcggccggtc
      781 ggtgccgttc ctcagcgtga aggtcgcgct atattcgtcg cccgaccgga ccaggggcgg
      841 caggcccgaa tagagcgtga ggtcctggac ggtccggatc gtggcgctgc cggtgccgaa
      901 caggtcgccg ccggcggtgg cgacggcgac gagcttgtag gccgagagcg agtcggccag
      961 cggaacgtcg agctcggccc ggccggcgga gtcgagcttg acccgcccgc gccacagcag
     1021 caccggcttg aagtcggtcc ggacgaggtc ggtgaggtcc cctcccccgc cgccgccggc
     1081 cgggacggcc ttgcggccgt aatggcgctt gccgaccacc tgcatcgccg ccgtcgaggt
     1141 cactaccgag agcgggcgct cgcccatcat cgccttgagc acgtcccagc tctcgttcgg
     1201 cgacagctgc agcagcgcct ggtcgaccgc ggcgaaggcg atctccgccg attgcgccgg
     1261 ccggccgttc gggcccagca cctggaccgc ggtccgcaca tggtcgcgga catgatagcg
     1321 ttccttgtcg ggccgaaccg cgaccttgag ctggtgcgct tcccacccga ccttcacccg
     1381 ggtgatgccg atccggtagc tcggcttggc gaggtcgacg agggcggtgg gattggcgat
     1441 ccgcccgagc accgggatct tccaccgctg cgcgaactcg gccatccaca cccgccattt
     1501 ggcgacccgt cccctgaccg ccatcaccga gatgtagacg tcgggggcgt aggcgccggg
     1561 cagcttgacg tcgatcaccg gctccttgcc cgacagctcg gtgacgaacg aggagagcac
     1621 cccctcgcgc tcgacggtga ccagggcggt cgccttgcgg aacggcatgc gcacctggac
     1681 gtgggcggtg tcgccgctgc ggtacgaatt cgaatccgcg atgatgtcca tgcggtcgcc
     1741 attgtcgccg ccgaaccacc agtcgtcgtc gccggcgagg tagacggagg tgaccgcccg
     1801 agcctcgttg ccctgcgggt cgcgggtcgt cgcgaccgcg atgacctcgc ccgagac
//
LOCUS       DASVQZ010000391         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_371495, whole genome shotgun sequence.
ACCESSION   DASVQZ010000391 DASVQZ010000000
VERSION     DASVQZ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371495"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VF547_11000"
     CDS             <1..1248
                     /locus_tag="VF547_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX8263389.1"
                     /translation="TRQKAGVKPTRLLPLLLVALIASLVLGRAVWAQLEGADRGVPPI
                     DSASTFEIGGIEVDVTADNAQKAREEGWRQAQQKGWKALWAKTNGRPAEQAPGLTDAA
                     LNGLVAGIVVENEQIGPKRYIATLGVLFDRARTGQLLGVSGSVRRSAPMLVIPVMLTG
                     SSFQSFESRNEWQKAWARFRTGSSPVDYVRPTGSGIDPLLLNVAQSRRPGRGWWRMLL
                     DQYGAADVIVPEVHLKRSYPGGPAVATFTARHGPDNRVLGQFTLRVGNSASIPRLLDE
                     GVRGLDLLYSRALAAGQLAPDPSLIIPEPPLVEEIEEETQVEETEAPPPTTGPSPVGA
                     VTVLRIEVDTPDQAAIGRAELAVSRVPGVTSAFTTDPDVGGVSSMRVSYMGEPAALAA
                     ALESQGWRVSGSGTSLRLSRGGD"
     gene            1250..1870
                     /locus_tag="VF547_11005"
     CDS             1250..1870
                     /locus_tag="VF547_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator DnaA"
                     /protein_id="HEX8263390.1"
                     /translation="MAQIALPLDWPVADRDEDFLISDSNRAAFDHLKRWSVWPVMATL
                     VTGPRKSGRSLLGRLFVRKTGGRLFDNAEDHDEEAIFHAWNEAQTSRRPLLIVADSPP
                     PIWTVALPDLRSRLAATPHVALGEPDDRLIGDLIVKLLGDRGVAVPPEVPAYVVPRIE
                     RSHLAVQQAVAALDRAALSHHRRMTVAMARRALAESGMIKRARSQD"
     gene            1871..>2617
                     /locus_tag="VF547_11010"
     CDS             1871..>2617
                     /locus_tag="VF547_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX8263391.1"
                     /translation="MNETAPSPAPEPPLAPRDRYFNRELSWLAFNRRVLEEARNPAHP
                     LLERLRFLSISGSNLDEFFMVRVAGLKAHRVLGVEEASADGLTPAQQLAAVAAEADEL
                     TQSQQQVWSELRRALADAGVAVIGGGAIDADSAEWLERHFREQIFPVLTPQAIDPAHP
                     FPFIPNRGFSLIFDLRHRRTGEPIRELLMVPATLSRFVRLPGAEARYIAVETLIRRNI
                     AAMFPDHELVGGGAFRILRDSDIEVEEEAED"
BASE COUNT          366 a          977 c          899 g          375 t
ORIGIN      
        1 actcgccaaa aggcgggcgt gaagccgacc cgccttctgc ctctcctgct cgtcgccctg
       61 atcgcctcgc tcgtcctggg gcgcgcggtc tgggcgcagc tcgagggcgc cgaccgcggc
      121 gttccgccga tcgacagcgc cagcaccttc gagatcggcg ggatcgaggt cgacgtcacc
      181 gccgacaacg cccagaaggc gcgcgaggag ggctggcgcc aggcccagca gaagggctgg
      241 aaggcgctct gggccaagac caacggccgg cccgccgagc aggcgcccgg cctcaccgac
      301 gccgctctga acggcctcgt cgccgggatc gtcgtggaga atgagcagat cgggccgaag
      361 cgctacatcg cgacgctcgg agtcctgttc gaccgggccc ggaccgggca gctgctcgga
      421 gtcagcggct cggtgcgccg ctcggcgccg atgctggtga tcccggtcat gctgaccggc
      481 tcgtccttcc agagcttcga atcgcgcaac gaatggcaga aggcctgggc gcgcttccgg
      541 accggcagca gccccgtcga ctatgtccga ccgaccggct cgggcatcga tcccctgctg
      601 ctcaacgtcg cccagtcgcg ccggcccggc cgcggctggt ggcggatgct gctcgatcaa
      661 tatggcgccg ccgacgtcat cgtgccggaa gtgcatctca agcgcagcta tccgggcggg
      721 cccgccgtcg ccaccttcac cgcccgccac ggccccgaca accgggtgct cggccagttc
      781 accctgcgcg tgggcaacag cgcctcgatc ccgcggcttc tcgacgaggg cgtgcgcggc
      841 ctcgacctgc tctacagccg cgccctcgcc gccggccagc tggcgccgga tccgtcgctg
      901 atcatcccgg agccgccgct cgtcgaggag atcgaggagg agacccaggt cgaggagacg
      961 gaggccccgc cgccgacgac gggcccctcg ccggtcggcg cggtcaccgt gctgcggatc
     1021 gaggtcgaca cgcccgatca ggcggcgatc ggccgcgccg agctggcggt gagccgggtt
     1081 cccggcgtca cctcggcctt caccaccgac ccggacgtgg ggggcgtctc gtcgatgcgg
     1141 gtcagctata tgggcgagcc cgcggcgctg gcggcggcgc tcgaatcgca gggctggcgg
     1201 gtttcgggct ccggcaccag ccttcgcctg tcgcggggcg gcgactgacg tggcgcagat
     1261 cgcgctgccg ctcgactggc cggtagccga ccgcgatgag gatttcctca tctccgattc
     1321 gaaccgggcc gccttcgacc atctcaagcg ctggtcggtc tggccggtca tggcgaccct
     1381 cgtcaccggc ccgcgcaagt cgggccggag cctgctcggc cggctgttcg tccggaagac
     1441 cggaggccgc ctgttcgaca atgccgagga ccatgacgag gaggcgatct tccacgcctg
     1501 gaacgaggcc cagacgtcgc gccggccgct gctcatcgtc gccgactccc cgccgccgat
     1561 ctggacggtg gccctgcccg acctgcgctc gcgcctcgcc gcgacccccc atgtcgcgct
     1621 cggcgagccc gacgaccggc tgatcggcga tctcatcgtc aagctgctcg gcgaccgcgg
     1681 agtcgccgtt ccgccggagg tgccggccta tgtcgtcccc cggatcgaac gctcccatct
     1741 ggccgttcag caggccgtcg ccgcgctcga ccgagcggcc ttgtcacatc accgccgaat
     1801 gaccgtggca atggcgcgca gggcgcttgc ggaaagcggc atgatcaaga gggcgagaag
     1861 ccaggactga atgaacgaga ccgcgccatc gcccgcgccc gagccgcccc tggcgccgcg
     1921 ggaccgctac ttcaaccgtg agctgagctg gctcgccttc aaccggcggg tcctcgagga
     1981 agcgcgcaac cccgcccacc cgttgctcga gcggcttcgc ttcctctcca tctccgggag
     2041 caacctcgac gaattcttca tggtgcgggt cgccgggctg aaggcgcacc gcgtcctcgg
     2101 ggtcgaggag gcctcggccg acggcctcac ccccgcgcag cagctcgccg cggtcgccgc
     2161 cgaggccgac gagctgacgc agagccagca gcaggtctgg tccgagcttc gccgggccct
     2221 ggccgacgcg ggcgtggcgg tgatcggcgg cggcgcgatc gacgccgatt cggcggaatg
     2281 gctggagcgc cacttccgcg agcagatctt cccggtgctg acgccccagg cgatcgatcc
     2341 ggcccatccc ttccccttca tccccaaccg gggcttctcg ctgatcttcg accttcggca
     2401 ccgccggacg ggcgagccga tccgcgagct gctgatggtg cccgcgaccc tgtcccgctt
     2461 cgtccgcctg cccggggcgg aggcccgcta catagccgtc gagactctga tccggcgcaa
     2521 catcgcggcc atgttccccg atcacgagct ggtcggcggc ggcgccttcc ggatcctgcg
     2581 cgacagcgac atcgaggtgg aggaggaggc ggaggac
//
LOCUS       DASVQZ010000392         9283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_757074, whole genome shotgun sequence.
ACCESSION   DASVQZ010000392 DASVQZ010000000
VERSION     DASVQZ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757074"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VF547_11015"
     CDS             <1..222
                     /locus_tag="VF547_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX8263392.1"
                     /translation="TGPVKRSVMIAGHATSISLEPVFWDALKAAAEAEGLPLSALVAR
                     IDAERVEAADPPNLASAIRVWLFERKDGR"
     gene            complement(349..2022)
                     /locus_tag="VF547_11020"
     CDS             complement(349..2022)
                     /locus_tag="VF547_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8263393.1"
                     /translation="MPSELDRKFDAVLAQVTGPGGRIQLAEDAEGRKIVANLPPTLPG
                     MFDAFCALHSATVAVIADGERITFGELNAQATQLARALAGGWGVGKGDRVAIAMRNCP
                     AWIVTYMAVLKAGAIATLVNGWWQAEELRHGLELTEPCLVLCDPPRANRLEATGLAIA
                     SVVLPVDRPIGEALAPLLGRGEADLPEVGPEDDATILFTSGSTGVAKGAISSHRAVTT
                     GIYTYAISLLALLGIMESEGRGPTNPPRTLVNVPLFHVTGEVPVLLNSFVIGRGMVLM
                     AKWDAGEALRLIQDEKVTYFVGVPTMSLELMQHPDREKFDLSSLTDIAAGGAARPVAH
                     VRRLQESFKGAQPALGYGLTETNAVGCGNFWQNYADKPASTGRPHKPTVELAILGEDD
                     RHLPVGETGEIAIRSAANFRGYWNDPAATAAAFTADGYLKTGDIGYIDEDGYLFIVDR
                     KKDIIIRGGENISCQEVEAAIYSHPAVSEASVFGVPDERLGEVPAAVIYCEDERGFDS
                     DALRDFLDGRIALYKIPQHIWVHDEPLPKLGTGKIDKKSLRERYSAEVG"
     gene            2110..2961
                     /gene="lgt"
                     /locus_tag="VF547_11025"
     CDS             2110..2961
                     /gene="lgt"
                     /locus_tag="VF547_11025"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX8263394.1"
                     /translation="MSAGVEHYVRWADLGLDPVLLELGPIKIRWYSLAYLAGILVGWW
                     YLMRLIARPGAPMARRHVDDLVFYATLGIILGGRLGYVLFYRPGIYFSDPVQILRIWD
                     GGMSFHGGVIGTTLGILWLARKHKLDWLRVHDYVACCTPFGLFFGRLANFVNGELWGR
                     PTDVPWAIVFPTGGDVPRHPSQLYEAGLEGLLLFAILWFLFWRTDARYQPGKLVGTFL
                     LVYGFSRFLVELVRQPDQGLEHLPWGLTMGQTLTVPMILGGAWLIATAKGRRQRVEPI
                     AGTESVA"
     gene            3059..4111
                     /locus_tag="VF547_11030"
     CDS             3059..4111
                     /locus_tag="VF547_11030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8263395.1"
                     /translation="MQGGGTLADRLRRLIEAEGPIPVSDYMAIANSHYYATRDPLGAA
                     GDFVTAPEISQMFGELIGLALADLWVRAGRPGGVRYVELGPGRGTLAADALRAMRAAG
                     LAPAVHFVETSPVLRAAQAERVPDAAWHDDVAALPADGPVLVLANEFFDALPVRQLIA
                     TPGGWRERRVGVEAGRFVPVAGAPVPSSAIPAHLRDAPAGTVIEASPASLACMAQLAD
                     RMTMQGGAAFIIDYGHDRTSAGETLQAVRAHRYADPWTEPGTADLTAHVDFEVLRESA
                     GRAGVRCWGPVPQGEWLLRLGLDSRARALIRAAPDREEEVLAARDRLVSPGEMGSLFR
                     VLGLSSAAWPAPEGFE"
     gene            4108..4614
                     /locus_tag="VF547_11035"
     CDS             4108..4614
                     /locus_tag="VF547_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8263396.1"
                     /translation="MISYRDLGPDDAAALSDLHRRSFVETFGHLYKPADLAAFLDRLD
                     QEAFRGELLDGEHQVRFAECDGEPVAFVKLGPITLPVQPSRPALEFRQLYVLRQWQGA
                     GIAQELAEWAIGQARRRGAEELYLSVFVENHRAKAFYRRYGFSYVGPYEFMVGEQADE
                     DEIWKLHL"
     gene            4596..5369
                     /gene="pgeF"
                     /locus_tag="VF547_11040"
     CDS             4596..5369
                     /gene="pgeF"
                     /locus_tag="VF547_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX8263397.1"
                     /translation="MEAAPVEALTSGALEGLRHGFLGRRGGVSQGVCSGLNVGWGSAD
                     DRDSIAENRRRALDRVAPGARLVTLHQVHSPDAVRVTEPWPDDARPRGDAMVTDRPGL
                     VLGILTADCAPILLADREAGVVAAAHAGWKGALGGVAEATVAQMERLGAVRARIAAAV
                     GPCIGRRSYEVDDAFLRRFAEADPDHERFFSPGREGRHQFDLEGFVVSRLAAAGVARI
                     EALGEDTYGQPDRFFSYRRATHRGEPDYGRQISLIGLDG"
     gene            complement(5416..6297)
                     /locus_tag="VF547_11045"
     CDS             complement(5416..6297)
                     /locus_tag="VF547_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263398.1"
                     /translation="MSPAAAGALTAVLVTVVIAAGTEPRGIPPAVIAAAISAAIALIV
                     LSVGWKTAWLQRAESIFRSVPSLTVEVEPSFLETPGEEDVILQTKVIVRNISRTSCTI
                     PAVYIRYHCLGVPPSQKHVGRVNADLLPKWGSMGQVVNAAWVHRAVVQLGPDEIEEFV
                     RWDTLDKVSVARAPTSIAVVDVFATTIKEIGGGIVPRSRPGRLRQRWLQFMSQDDCVR
                     NTEVIFARYDPEIHGRHPELDIYQRVIVRPGSEGTAVDLENTRIFKPILRHMLRWTRY
                     QNLNVLGARGPQGAGKG"
     gene            6482..7885
                     /locus_tag="VF547_11050"
     CDS             6482..7885
                     /locus_tag="VF547_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX8263399.1"
                     /translation="MAGRWGLWGGLALFVALLALPAPAGMSPAAWRTTAIIVLMACWW
                     MTEALPLTATALVPFLAFPLLGIMTATDSAAAYYSPILFLVLGGAIIALAIERTGLHR
                     RLALAIVSRGGGGPAQMLFAFMAATALLSTIVSNTATTLIMMPIGLAVLRAARVEPGH
                     TDGFAGALAMGIAFAATIGGFGTLVGSPTNAIAAGIIEKSTGYRIDFLTWALYGIPLV
                     LLCVPLCWAILMKVQKVAATDFDPAAARAGIGETGRWSAAEKRLVPIVALTVAAWIAV
                     PFVTPYLPKDSLTDGTIAVVAALLLFVIPDGTGRAILNWEEANRAPWGVIMMFGGGLA
                     LAAGIGESGLADWMGIALQPLRSVHPLIVAAVLVGIVIVVTEFASNVATASGAIPVVA
                     GIIAATGIDPILLALPAAFASSWGFMLPSGTGPNAIAWATGHIALPRMLRAGLLMDLA
                     GVPLMVGVVWGIGAVLG"
     gene            8121..>9283
                     /locus_tag="VF547_11055"
     CDS             8121..>9283
                     /locus_tag="VF547_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX8263400.1"
                     /translation="MAEDESDLTGATSRRRPKASALEIGGRRLKPSTLMMGYGYDPTL
                     SEGSLKPPVFLTSTFVFENAAAGKRFFEGVTGKRPGGAEGLVYSRFNGPNQEILEDRL
                     SLWEDAEDALTFSSGMSAISTMLLAFVQPGDVVVHSGPLYAATETLIARILGRFGVKW
                     LDFPAGATREEIEAVLERAKALGRVSLIYLESPANPTNALVDVEAVAAARSAVLGEER
                     PPIAIDNTFLGPLWAQPLRQGADLSVYSLTKYAGGHSDLVAGGITGSKELINTIRMLR
                     NTIGTITDPNTAWMLLRSLETLELRMMRAGENADKVCRFLRSHPKVETVGYLGFLEEG
                     GDERQADIYRRHCTGAGSTFSLYLKGGEREAFAFLDSLRIAHLAVSLGGTETLA"
BASE COUNT         1282 a         3342 c         3118 g         1541 t
ORIGIN      
        1 acgggccccg tgaagcgctc ggtgatgatc gccggccacg ccacgtcgat cagcctcgag
       61 ccggtgttct gggacgcgct caaggcggcc gccgaggccg aggggctgcc gctgtccgcc
      121 ctggtggcgc gcatcgacgc cgagcgcgtc gaggcggccg atcctcccaa tctcgccagc
      181 gcgatccggg tctggctatt cgagcgcaag gacggccgct gaaccgatcc cctctccctg
      241 cagaggagag ggaaaagccg cgtcagcggc cgagggtgag ggcaaggcgc cgcgccctca
      301 cccagcgtcg cttcgctccg ctgtcgctct cccctgcagg gagaggggtt atcccacctc
      361 cgccgaataa cgctcgcgga gcgacttctt gtcgatcttg cccgtgccga gcttgggcag
      421 gggctcgtcg tgcacccaga tatgctgcgg tatcttgtag agcgcgatcc ggccgtcgag
      481 gaaatcgcgc agcgcgtcgg aatcgaagcc gcgctcgtcc tcgcaataga tgaccgccgc
      541 cggcacctcg ccgagccttt cgtcgggcac tccgaacacc gacgcctccg acaccgccgg
      601 gtgcgagtag atcgccgcct cgacctcctg gcagctgatg ttctcgccgc cgcggatgat
      661 gatgtccttc ttgcggtcga cgatgaacag atagccgtcc tcgtcgatat agccgatgtc
      721 gccggtcttc agatagccgt cggcggtgaa cgccgccgcg gtggccgccg ggtcgttcca
      781 atagccgcgg aaattggccg ccgagcggat cgcgatctcg ccggtctcgc ccacaggaag
      841 gtggcggtcg tcctcgccca ggatggccag ctcgacggtg ggcttgtggg gccgcccggt
      901 ggaggcgggc ttgtcggcgt aattctgcca gaaattaccg cagcccacgg cattggtctc
      961 ggtgagcccg tagcccagcg ccggctgggc gcccttgaag ctctcctgca ggcgccggac
     1021 atgcgccacc ggccgggccg ctccgccggc ggcgatgtcg gtaagcgagg aaaggtcgaa
     1081 cttctcccgg tcggggtgct gcatgagctc gaggctcatc gtcggaacgc cgacgaaata
     1141 ggtgaccttc tcgtcctgga tcagccggag cgcctcgccc gcgtcccact tcgccatcag
     1201 caccatgccc cggccgatga cgaagctgtt gagcagcacc ggcacctcgc cggtgacgtg
     1261 gaagaggggc acgttgacca gggtccgcgg cgggttggtc ggaccgcggc cctcgctttc
     1321 catgatcccg agcaaggcca gcaggctgat cgcataggtg tagatgccgg tggtgacggc
     1381 ccggtggctc gagatcgcgc ccttggcgac gccggtcgag cccgaggtga acaggatggt
     1441 ggcgtcgtcc tccggcccga cctcgggcag gtccgcctcg ccgcgcccga gcagcggcgc
     1501 cagcgcctcg ccgatcggcc ggtccaccgg cagcaccacg ctcgcgatcg cgagtccggt
     1561 cgcctcgagc cggttggcgc ggggcgggtc gcagagcacg aggcacggct cggtcagctc
     1621 gaggccgtgg cgcagctcct cggcctgcca ccagccgttg acgagcgtgg cgatcgcgcc
     1681 cgccttcagc accgccatgt aggtgacgat ccaggccggg cagttgcgca tcgcgatcgc
     1741 gacccggtcc cccttgccga cgccccagcc ccccgccagc gcgcgcgcca gctgcgtggc
     1801 ctgggcgttc agctcgccga aggtgatgcg ctcgccgtcc gcgatcaccg cgaccgtggc
     1861 cgaatggagg gcgcagaagg cgtcgaacat gccgggcagg gtcggtggca ggttggcgac
     1921 gatcttgcgc ccttcggcat cctcggcgag ctggatgcgg ccgccggggc ccgtcacctg
     1981 cgccagcacc gcgtcgaact tgcggtccag ctcgctcggc atccgactct ccctttccgt
     2041 ttcttcctcc tcctcctaat agggaatttg ccggaggggg aagccttgat cgatccgtta
     2101 cttacaagct tgtcggcggg cgtcgaacat tatgtccgct gggccgatct cggcctcgat
     2161 ccggtcctcc tggagctcgg gccgatcaag attcgctggt attccctcgc ctatctggcg
     2221 ggcatcctgg tcggctggtg gtacctgatg cggctgatcg cgcggccggg cgcgccgatg
     2281 gcgcggcggc acgtcgacga tctcgtcttc tacgccacgc tcggcatcat cctgggcggc
     2341 cgcctcggct acgtgctctt ctaccggccc ggcatctatt tctccgatcc ggtgcagatc
     2401 ctccgcatct gggacggcgg catgtccttc cacggcggcg tgatcggaac cacgctcggc
     2461 atcctgtggc tggcgcgcaa gcacaagctc gactggctca gggtccacga ctacgtcgcc
     2521 tgctgcaccc cgttcggact gtttttcggc cgcctcgcca acttcgtgaa cggcgaactg
     2581 tggggacgcc cgaccgacgt cccgtgggcg atcgtcttcc cgaccggcgg cgacgtgccc
     2641 cgccatccca gccagctcta cgaggccggc ctcgaaggcc tgctcctgtt cgccatcctc
     2701 tggttcctgt tctggagaac ggacgcccgc taccagcccg gcaagctggt cggcaccttc
     2761 ctgctcgtct acggtttcag ccgcttcctc gtcgagctgg tccgccagcc ggaccaggga
     2821 ctggagcatt tgccctgggg gctcaccatg gggcagaccc tgaccgtgcc gatgatcctc
     2881 ggcggcgcct ggctgatcgc caccgcgaag ggccggcggc agagggtcga gccgatcgcc
     2941 gggacggaga gcgtcgctta ggattcccct ccctgcacgc aggaaggggc taggggtggg
     3001 caggtcgatg acggtcggtg attcttcgga ggcgcaccca cccccggccc ctccctgcgt
     3061 gcagggaggg gggaccctcg ccgatcgcct gcgccgcctg atcgaggccg agggcccgat
     3121 ccccgtctcc gattacatgg cgatcgccaa ctcccattat tacgccaccc gcgacccgct
     3181 cggcgccgcc ggcgatttcg tgaccgcgcc cgagatcagc cagatgttcg gcgagctgat
     3241 cggcctcgcc ctcgccgacc tgtgggtccg cgccggccgg cccgggggcg tgcgctacgt
     3301 cgagctcggt ccgggccgcg gcaccctcgc cgccgatgcg cttcgggcga tgcgggcggc
     3361 cggcctcgct cctgccgtcc atttcgtcga gaccagcccg gtgcttcgag ccgcccaggc
     3421 cgagcgggta cccgacgccg cctggcacga cgacgtcgcc gcgctgccgg ccgacggccc
     3481 ggtgctggtc ctcgccaacg agtttttcga cgccctcccc gtccgccagc tcatcgccac
     3541 gcccggggga tggcgggagc gccgggtcgg cgtcgaggcc gggcgcttcg tgcccgtcgc
     3601 cggcgcgccg gtgcccagct cggcgatccc ggcccatctt cgcgatgccc ccgcgggcac
     3661 ggtgatcgaa gcttcgccgg cctccctggc gtgcatggcc cagctcgccg atcgcatgac
     3721 catgcaaggc ggagccgcct tcatcatcga ttacggtcac gatcgcacct cggccggcga
     3781 gaccttgcag gcggtgcgtg cgcatcgata tgcagatccc tggacggagc cgggaacggc
     3841 cgacctcacc gcccatgtcg acttcgaggt attgcgggaa tccgcaggcc gggccggcgt
     3901 gcgctgctgg ggcccggtcc cgcagggcga atggctgctc aggctcggcc tcgattcgcg
     3961 ggcgcgcgcg ctgatccgcg cggcgccgga ccgcgaggag gaggtgctcg ccgcgcgcga
     4021 ccggctggtt tcgcccgggg agatgggttc cttgttccgg gtgctcggcc tttcgtccgc
     4081 ggcctggccg gctccggagg gctttgaatg atctcctatc gcgacctcgg ccccgacgac
     4141 gccgccgcgc tgtccgatct ccaccgccgc agcttcgtcg agacgttcgg ccacctctac
     4201 aagcccgcgg acctcgccgc cttcctcgat cggctcgacc aggaggcctt tcgaggcgag
     4261 ctgctcgacg gcgagcacca ggtccgcttc gccgaatgcg acggggagcc cgtcgccttc
     4321 gtcaagctcg ggccgatcac cctcccggtc cagccctcgc gtccggccct ggaattccgc
     4381 cagctctacg tgctccggca gtggcagggt gcggggatcg cgcaggagct ggccgaatgg
     4441 gcgatcggcc aggcgcgccg gcgaggcgcc gaggagctct atctctcggt cttcgtcgag
     4501 aaccaccgcg ccaaggcctt ctaccgtcgc tacggcttct cctatgtcgg cccctacgag
     4561 ttcatggtcg gcgagcaggc ggacgaggac gagatatgga agctgcacct gtagaggcgt
     4621 tgacgtccgg cgccctggaa ggccttcgcc acggcttcct cggccgccga gggggcgtgt
     4681 cgcaaggggt ctgctccggc ctcaacgtcg gctggggctc ggcggacgac cgcgactcga
     4741 tcgccgagaa tcgccgccgg gccctggacc gagtggcgcc gggcgcccgc ctggtaacgc
     4801 tccaccaggt ccattcgccg gacgcggtcc gcgtcaccga gccctggccc gacgacgccc
     4861 gcccgcgcgg cgacgcaatg gtgaccgacc ggcccggcct cgtcctcggc atcctcaccg
     4921 ccgactgcgc gcccatcctc ctcgccgacc gcgaggcggg ggtggtcgcc gcggcccacg
     4981 ccggctggaa gggggcgctc ggcggcgtgg ccgaagcgac ggtggcgcag atggagcggc
     5041 tcggcgccgt ccgcgccagg atcgccgccg ccgtgggccc gtgcattggc cgccggtcct
     5101 acgaagtgga cgacgccttc ctgcgccgct tcgccgaagc cgatcccgac catgagcgct
     5161 tcttctcgcc gggccgcgaa ggacgccatc agttcgatct ggaaggcttc gtcgtctcgc
     5221 gcctcgccgc cgccggcgtc gcccggatcg aggcgctcgg cgaggacacc tacggccagc
     5281 ccgaccgctt cttcagctac cgccgggcca cgcatcgcgg cgagcccgat tacgggcgcc
     5341 agatctcgct gatcggcctg gacggatgaa agtccgatgc gttcggcccg ccgggagacg
     5401 tcgagggctt tccgctcacc ctttgcccgc gccctgcggc cccctcgctc cgaggacgtt
     5461 caggttctga tatcgagtcc agcgaagcat gtggcgcaat atcggtttga atattcgggt
     5521 gttttcgaga tcgaccgcag tcccttccga gccggggcgg acaatgaccc tctgatagat
     5581 atcgagttcg gggtgccggc cgtggatctc cggatcgtac cgggcgaata tgacttcggt
     5641 gttccgcacg caatcgtcct ggctcatgaa ctgcagccag cgctgccgca ggcgcccggg
     5701 gcgggaccgg gggacgattc cgcctccgat ctcctttatc gtcgtcgcga aaacgtcgac
     5761 cacggcgatc gatgtcggcg ctcgcgccac gctcaccttg tccagcgtgt cccaccggac
     5821 gaattcctct atttcgtccg gccccagctg gaccacggcc cgatgaaccc aggccgcgtt
     5881 gacgacctgg cccatgctgc cccatttggg cagaaggtcg gcattcacgc gcccgacgtg
     5941 cttttgggag ggcgggactc cgaggcagtg atatctgatg tagactgcag gaatggtgca
     6001 cgaagtccgt gagatgttcc tgactatcac cttggtctgc aggatcacgt cttcctcgcc
     6061 cggagtctcg agaaaactgg gctcgacctc caccgtaagg gaggggacgc tcctgaatat
     6121 gctttcggcc cgttgaagcc aggcggtctt ccagccgacc gacaggacga tcagggcgat
     6181 ggcggccgat atggcggcgg cgatcacggc cggcggtatt ccgcgcggct cggtgcccgc
     6241 ggctatgacg acggtcacca ggaccgccgt cagcgcgccg gcggccgccg gggacatttc
     6301 ttggctgggg agcaatccgc ctatctccac atggatgagc cgagccggct tcctcccgga
     6361 gccagaccgt gatgcacgac tctgcccggg agagtagaaa ggagcttgcg gactgtccac
     6421 tcgccgcgct ggccagccac cgtcggctct ggcactacag gcggcgagac ggggtgggac
     6481 gatggccgga cgctggggat tgtggggcgg gctcgccctg tttgtggcgc tgctggcgct
     6541 gccggcgccc gcaggcatgt cgccggccgc ctggcgcacg accgcgatca tcgtgctgat
     6601 ggcctgctgg tggatgaccg aggcgctgcc gctcaccgcc accgcgctcg tgccgttcct
     6661 cgcctttccg ctgctcggga tcatgaccgc gaccgattcg gccgccgcct attattcgcc
     6721 gatcctgttc ctggtgctcg gcggcgcgat catcgcgctg gcgatcgagc ggaccgggct
     6781 gcatcgccgc ctggcgctcg ccatcgtcag ccgcggcggc ggcggcccgg cgcagatgct
     6841 gttcgccttc atggccgcca ccgccttgct cagcacgatc gtgtccaaca cggcgacgac
     6901 gctcatcatg atgccgatcg gcctcgccgt cctccgcgcg gcgcgggtcg agcccggcca
     6961 caccgacggc ttcgccggag cgctcgccat gggcatcgcc ttcgccgcga cgatcggcgg
     7021 cttcggcacg ctcgtcggct cgccgaccaa cgccatcgcc gccgggatca tcgagaagtc
     7081 gaccggctac cggatcgatt tcctcacctg ggccctctac gggattccgc ttgtccttct
     7141 gtgcgtgccg ctgtgctggg cgatcctgat gaaggtgcag aaggtcgccg cgaccgattt
     7201 cgaccccgcc gcggcccggg ccgggatcgg cgagaccggc cgctggtcgg ccgccgagaa
     7261 gcgcctcgtc ccgatcgtcg ccctcaccgt ggcggcgtgg atcgcggtcc cgttcgtcac
     7321 gccctacctc ccgaaggatt cgctgaccga cggcaccatc gccgtcgtgg cggcgctgct
     7381 cctgttcgtg atccccgacg gcaccgggcg ggccatcctc aactgggaag aggccaatcg
     7441 cgcgccctgg ggcgtgatca tgatgttcgg cggaggcctc gcgctcgccg ccgggatcgg
     7501 cgaatcgggg ctcgcggact ggatgggaat cgcgcttcag ccgctgcgat cggtccaccc
     7561 cctgatcgtg gcggcggtgc tcgtcgggat cgtcatcgtc gtcaccgaat tcgcctccaa
     7621 cgtggccacg gcgagcggcg ccatacccgt cgtcgccggg atcatcgccg ccacgggcat
     7681 cgatccgatc ctgcttgccc tccccgccgc cttcgcctcg agctggggct tcatgctgcc
     7741 ctcgggcacc gggccgaacg cgatcgcctg ggcgaccggc cacatcgctc tgccgcgcat
     7801 gctgcgcgcg ggcctgctga tggaccttgc cggagtgccg ctgatggtcg gggtggtgtg
     7861 ggggatcgga gcggtcctgg gctgaaccgt cgccccagcg caggctgggg ccccggcggg
     7921 cgaagcagac cgcgcctttc atcccctggg gtcccaccct tcgctgggac gacgaggggc
     7981 tagcggcgcg cccgcctgat gggttaggaa gcgctcgggc aacggacctc acccctccgg
     8041 gccgctttcg agcgggcgcc ggtgagcctc tcacgggctc ggtctccacg cagatctgcg
     8101 acctgacagg agacattccc atggctgaag acgaatccga cctcaccggc gcgacttcgc
     8161 ggcggcgccc caaggccagc gcgctcgaga tcggcgggcg gcggctgaag ccttccacct
     8221 tgatgatggg ctacggctac gacccgacgc tttcggaagg ttcgctgaag cccccggtct
     8281 tcctcacctc gaccttcgtc ttcgagaacg ccgccgccgg caaacgcttc ttcgagggcg
     8341 tcaccggcaa gcgcccgggc ggggccgaag gcctggtcta ttcgcgcttc aacggcccca
     8401 accaggagat actcgaggac cgcctcagcc tgtgggagga cgccgaggac gccctgacct
     8461 tctcctcggg catgtcggcc atctcgacca tgctcctcgc cttcgtccag cccggcgacg
     8521 tcgtcgtcca ctcgggcccg ctctacgccg cgaccgagac tctgatcgcc cggatcctgg
     8581 gccgattcgg ggtcaaatgg ctcgatttcc cggccggagc gacccgcgag gagatcgagg
     8641 ccgtgctcga gcgcgccaag gcgctcgggc gggtgtcgct catctacctg gaaagcccgg
     8701 ccaacccgac caacgccctc gtcgacgtcg aggcggtcgc cgccgcgcgc tcggcggtgc
     8761 tgggcgagga gaggccgccg atcgcgatcg acaacacctt cctcggcccg ctctgggccc
     8821 agccgctgcg ccagggcgcc gacctctcgg tctattcgct gaccaaatat gccggcggcc
     8881 acagcgatct cgtcgccggc gggatcaccg gatcgaagga gctcatcaac accatccgga
     8941 tgcttcgcaa cacgatcggg acgatcaccg atcccaacac ggcctggatg ctgctccgga
     9001 gcctcgagac gctcgagctg cgcatgatgc gggccggtga gaatgcggac aaggtctgcc
     9061 gcttcctgcg gagccatccc aaggtcgaga ccgtcggcta tctcggcttc ctggaggaag
     9121 gcggcgacga gcgccaggcc gacatctacc gccgtcattg caccggcgcc ggctcgacct
     9181 tctcgctcta cctgaagggc ggcgagaggg aggccttcgc cttcctcgat tcgctccgca
     9241 tcgcccatct cgcggtgagc ctgggaggca ccgagaccct cgc
//
LOCUS       DASVQZ010000393        10360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_660754, whole genome shotgun sequence.
ACCESSION   DASVQZ010000393 DASVQZ010000000
VERSION     DASVQZ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660754"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF547_11060"
     CDS             <1..690
                     /locus_tag="VF547_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEX8263401.1"
                     /translation="INTERAAMWKTMGQLEQMIHISKQLDKKLEDKANELDATEPAKA
                     KAIRETALFYTRQRTTDLLTQMAVTVQGYLALDLVKKNNVELIKGVDRASTTTVAALR
                     TAVTVAQAMTNQRLVLEQITSLNTTTANIIDSTGELLKSQTGEIHRQAASSTIPLETL
                     QRAFQNIYDTMDTIDRFKVEALGSMKQTVDTLSSEVEKSRGYIARAEGTEQNRLAGRS
                     SGSLLLNPIES"
     gene            694..1422
                     /locus_tag="VF547_11065"
     CDS             694..1422
                     /locus_tag="VF547_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263402.1"
                     /translation="MARSVDEAIARFEDYKSRLDGYGPAATNVRRRYYGRAMGSLVRR
                     VATMGMAIGLLIVATIGFGLIVGPIGWTGLFLVGLATLAILLFFSFWPSEPKRVPYNE
                     QVPTGTVVRQLESMLVRRRAALPAPAAQRADAIGAQLPLLESRLAEVDPLDPLAQDAR
                     RLMGKHLPELIERYERIPAAYRHQADGEGLTADQRLVASLDAARQALGDIGDRLVQGD
                     RQAFETQGRFIESRYKEGSGPGGE"
     gene            1419..2147
                     /locus_tag="VF547_11070"
     CDS             1419..2147
                     /locus_tag="VF547_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263403.1"
                     /translation="MMGFDLAGLWRQADEFLRLHLKSKAVREAEKRRAERQSREAGRR
                     MRRAALAGGASGAGIVAYGALAAPLAGPALIAAGGGALLLVAVALSLPTRRRIAGGDF
                     SRRELAELPGDAEDWLLARRDSLPLGCRPLLDRIFRRLGDLQPHLATLAPHSGSAWEA
                     RRLLGDHLPRLVLAYEGIPPSGRKEDPAVEARLCSGLAAIADALDALCREVCRDPLMT
                     FETQGRFIESRYRGESKTPSNLCE"
     gene            complement(2105..2776)
                     /gene="gspN"
                     /locus_tag="VF547_11075"
     CDS             complement(2105..2776)
                     /gene="gspN"
                     /locus_tag="VF547_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEX8263404.1"
                     /translation="MRLRLRWWRELFFLGALLFALVALLPLRFAVDSLGFAERGLTAR
                     SAAGSLWVGALDEAQLGPVPLGTVATRLRVLPLLAGRARLDVAQEGDGFRAGLTASRH
                     GFGIDDASGTIEVAGFGAVPPATLDLSDVSARFADGQCAAAEGMVKVRLAGQLGAAPL
                     VPGFSGEARCDGQAVLLPLRSQSGADRLDVRLFADGRYQLDASIRAGGSAYSQRFEGV
                     FDSPR"
     gene            complement(2776..3255)
                     /gene="gspM"
                     /locus_tag="VF547_11080"
     CDS             complement(2776..3255)
                     /gene="gspM"
                     /locus_tag="VF547_11080"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX8263405.1"
                     /translation="MSARLKALWLARTPRERWLLAVMLALGALVLVWFLVVRPLGDML
                     SAARQRHGDAVATLAETRRRAAEIAMLERGGPAVMEGSIDQAVAAAASEAGFQLSALQ
                     PQGPGRVSFALGAARPQPLFSWISALEGQGYVVERLSATSNPDRTLSAQIVLRARGR"
     gene            complement(3252..4340)
                     /gene="gspL"
                     /locus_tag="VF547_11085"
     CDS             complement(3252..4340)
                     /gene="gspL"
                     /locus_tag="VF547_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEX8263406.1"
                     /translation="MNDALLLFLARGGGVEGWLRIADGAVAARGAGIDGSEAHRGAPA
                     VAVAPGEEVTLRWLDLPAGLSPAQAAAAARLMAGELAGQPLDELHVAAGRETWDGARC
                     VGLVPMETMRAWLASVEAAGFEAERIIPETLLLPAPEEGFHVRDSGPLRLYRARDEAF
                     AVEPELGELLLAGRDAVLLDGEAGLAEALAEAPLDLRQGPFARRRDWRVAPARMRRLA
                     VLAAAFLLVSLAVQVAAIARYIFAADAAEAETRRVAAAALPRSPGVADPEAALTQRLA
                     ELRGGGAGFGATAGALFDAVKATPNVELSALAFSPDGLLRATVQADSPAAIEALSQRV
                     EANGFAAEVGAPRPGGGRRVADLMVRPR"
     gene            complement(4337..5329)
                     /gene="gspK"
                     /locus_tag="VF547_11090"
     CDS             complement(4337..5329)
                     /gene="gspK"
                     /locus_tag="VF547_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX8263407.1"
                     /translation="MNRPVPASERGAALLAVLVLVVIMSAIAAGAFERLRLSTAMAMN
                     GAALDQARAYALGVEDLLALRIDDLAGEDAYVTTLAGDWNGTARRIPLPGEGLAIGTI
                     RDGGNCFNLNSLVQGDVATTLVQRPAAIEQFVGLMLVLGLPEPSARRVAAASADWIDS
                     DSETGPQGAEDSAYAGGEPAYRAGNTLLADPSELRAVAGVTPELYRQLRPYLCALPAA
                     DMSPINVNTLLPRDAPLLAMFAPTKTRVDRARGLIAARPPAGWRNTYDFWRSAEQAGL
                     ETNADPMSQLQLKTYWFTIDLRVAFQGAEMEEWALVDARLAPAKVVARRWGGSE"
     gene            complement(5326..6060)
                     /gene="gspJ"
                     /locus_tag="VF547_11095"
     CDS             complement(5326..6060)
                     /gene="gspJ"
                     /locus_tag="VF547_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEX8263408.1"
                     /translation="MRVALQIPPRNGEGDPAKQGGGGPPLRCAEAGTPPPPPSAAVPL
                     PVPGRSDGGFTLVELLVSLFIFGLLAAAGVGLLSFSVRAQEAAEARLGDLADFRRAGA
                     LLAGDLAQAAPRLARDSSGNSRPAFHGGTGEQGGVALAFVRRGWENLDEAPRASLQRV
                     EYSVVDGRLERRIYPRLDGAAPFPATTVVDGVRRVRLRYRDGEGNWRERWDPTKASEL
                     PRAVELVLDAEGSGTTRQLFQTGTSG"
     gene            complement(6057..6425)
                     /gene="gspI"
                     /locus_tag="VF547_11100"
     CDS             complement(6057..6425)
                     /gene="gspI"
                     /locus_tag="VF547_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX8263409.1"
                     /translation="MSSPDEAGFTLIEMLVALAVFSLAALALLRLEGATVSTTALLRE
                     QALAQVVARNLAVETLTDPRPPAFGATRGEAINAGRKWRWLRTVGRSPEPRIQQIRIE
                     VRSDRGPEAANLTLFRRGPR"
     gene            complement(6412..6879)
                     /locus_tag="VF547_11105"
     CDS             complement(6412..6879)
                     /locus_tag="VF547_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX8263410.1"
                     /translation="MLRMVPLPVPGRSNGGFTLVELMVVLVIVGLVSAAVVLAMPDPR
                     GALRQEALRLAARTDAARERAMMDNRPVALVLGEGGYGFEWRTEGEWRPIAQRPFVAQ
                     DWSEGTRASIEGGEARVVFDSTGFAEPARLRLVRGREEAVVEVSDGGRVHVLP"
     gene            complement(6997..7359)
                     /gene="gspG"
                     /locus_tag="VF547_11110"
     CDS             complement(6997..7359)
                     /gene="gspG"
                     /locus_tag="VF547_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX8263411.1"
                     /translation="MVELMVVIVIIGLLATVVIINVLPATDKAAITKAKADVATLEQG
                     VEMYRLTKLRYPSASEGLQAVSSEGYVKRLPKDPWGNPYRYAAPGRDGRAFDIYSYGA
                     DGREGGQGQDADIGNWDS"
     gene            complement(7518..8744)
                     /gene="gspF"
                     /locus_tag="VF547_11115"
     CDS             complement(7518..8744)
                     /gene="gspF"
                     /locus_tag="VF547_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX8263412.1"
                     /translation="MSEFAYLVLDRAGRERRGSLRAATPDEAREKLSARGLFVVRMES
                     ASGEAAPALLSRKLFVRKKLNARQLTVFTRQLSTLVQVSPLEEALRTIARQTEKDQVR
                     DVLLAVHGSVLEGRRLSEAMAREPASFPPLYRGMVAAGEGSGTLPAILERLAGLLERQ
                     GQVRSKVLSALAYPAVLAIVAAFVVFALMVFVVPRVVEQFEDIGQQLPFLTRAVIAVS
                     HLLAGWWWALLLGLVLIGFAASRALRDERLRLRFDRALLRVPVIGRLMRDLHAARMAR
                     TLSTMVASRLPLLEGLKLTTETVHNRALRAATAQIAEAVRTGGSLSGALKRSGIFPPL
                     LVYLAASGEAGGRLDLMLERAADYLEREFDSFTTTALALLEPAIIVLMGALVAVIVLS
                     ILLPILQLDTLAGGMG"
     gene            complement(8737..10191)
                     /locus_tag="VF547_11120"
     CDS             complement(8737..10191)
                     /locus_tag="VF547_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX8263413.1"
                     /translation="MFAKRNGVALVGEANGQARVALRQGADPRALIEARRVLGRPLAV
                     ESATAEGFDKLLSERYAMDEQAAADAAGSLGLGDELSHLATDLPTADDLLDSADDAPA
                     IRLINGIIADAARQGVSDIHIEPYETGLVVRMRIDGVLRETLRMPAHVAPVVVSRIKV
                     MARLDIAERRVPQDGRIGLTLGGKLLDVRVSTLPSRAGERVVLRILDKENAGIDLDSL
                     GMPPSVHRLLRDALAEPNGIVLVTGPTGSGKTTSLYAGLRLLNDGSRNILTVEDPVEY
                     AVDGVGQTQVNPKVGLSFAAGLRAILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVL
                     STVHTNDAIGAITRMRDMKVEPFLLASTLRAVIAQRLVRRLCPACRAPVAADAATAAM
                     IGLPAGTIVHEPRGCAECGNSGFKGRTGVFEAVRVDDNVRRLINAGGDEVEMAAHAFR
                     NAPNLAAAARELVREGITSVGEAVRISRRESEDV"
BASE COUNT         1496 a         3861 c         3675 g         1328 t
ORIGIN      
        1 atcaacaccg agcgcgccgc gatgtggaag acgatggggc agctcgagca gatgatccat
       61 atctccaagc agctcgacaa gaagctcgag gacaaggcga acgagctcga cgcgaccgag
      121 ccggccaagg ccaaggcgat ccgcgagacc gccctcttct acacccgcca gcgcaccacc
      181 gacctgctca cccagatggc ggtgacggtc cagggctatc tcgccctcga cctcgtcaag
      241 aagaacaatg tcgagctgat caagggcgtc gaccgggcct cgacgacgac ggtcgccgcg
      301 cttcgcaccg ccgtcaccgt cgcccaggcg atgaccaacc aacgcctcgt gctcgagcag
      361 atcacctcgc tcaacacgac caccgccaac atcatcgatt ccaccggcga gctgctcaag
      421 agccagaccg gcgagatcca ccgccaggcc gcctcgtcga ccattccgct ggagacgctg
      481 cagcgcgcct tccagaacat ctacgacacg atggacacga tcgaccgctt caaggtcgag
      541 gcgctgggca gcatgaagca gaccgtcgac accctctcca gcgaggtcga gaagagccgc
      601 ggctacatcg cccgggccga gggaaccgag cagaaccgcc tcgccggccg cagctccggc
      661 tcgctgctgc tcaatccgat cgagagctga gccgtggccc gcagcgtcga cgaggcgatc
      721 gctcgcttcg aggactacaa gtcccggctc gacggctacg gcccggccgc cacgaacgtg
      781 cgccggcgct attacgggcg ggcgatgggc agcctggtgc gccgggtcgc gaccatgggc
      841 atggcgatcg ggctgctgat cgtggcgacg atcggcttcg gcctcatcgt cggtccgatc
      901 ggctggacgg gcctgttcct cgtcggcctg gcgacgcttg ccatcctgct cttcttctcc
      961 ttctggccca gcgagcccaa gcgcgtcccc tataacgagc aggtgccgac cgggacggtc
     1021 gtccggcagc tcgagagcat gctcgtgcgc cggcgcgccg ccctgccggc gccggcggcg
     1081 cagcgcgccg acgcgatcgg tgcccagctg cctttgctcg aatcgcgcct cgccgaggtc
     1141 gatccgctcg atccgctcgc ccaggacgcg cggcggctga tgggcaagca tctgcccgag
     1201 ctgatcgagc gctacgagcg catcccggcg gcctatcggc accaggccga cggcgagggc
     1261 ctgaccgccg accagcggct cgtcgccagc ctcgacgccg cccgccaggc gctcggcgac
     1321 atcggcgacc ggctggtcca gggcgaccgc caggcgttcg agactcaggg ccgcttcatc
     1381 gaaagccgct acaaggaagg cagcggcccg ggaggcgaat gatgggattc gatctcgccg
     1441 gcctgtggcg gcaggcggac gaatttctga ggctccacct caagtcgaag gcggtccgcg
     1501 aggcggagaa aaggcgggcc gagcgccagt cgcgcgaggc cggccgccgg atgaggcgag
     1561 cggccctggc cggcggcgcg tcgggcgccg gcatcgtcgc ctatggcgcg ctggccgcac
     1621 ccttggcggg gccggcgctg atcgcggcgg gcggcggcgc cctgctgctc gtcgccgtcg
     1681 ccctgtcgct gccgactcgc cggcgcatcg ccggcggcga tttctcgcgg cgcgagctgg
     1741 ccgagctgcc cggcgatgcc gaggattggc tgctcgcccg ccgcgattcc ctgccgctcg
     1801 gctgccggcc gctgctcgac cgcatcttcc gccgcctcgg cgatctccag ccgcatctcg
     1861 ccacgctcgc gccccattcg ggctcggcct gggaggcgcg gcggctgctc ggcgaccacc
     1921 tgccgcggct ggtcctcgcc tacgaaggga ttcccccgtc gggccggaag gaggatccgg
     1981 cagtcgaggc ccgcctctgc agcggcctcg ccgcgatcgc ggacgcgctc gacgcgctgt
     2041 gccgcgaggt gtgccgggat ccgctgatga ccttcgagac ccagggccgc ttcatcgaat
     2101 cccgctatcg gggagaatcg aagaccccct cgaacctctg cgaataggct gagccgcccg
     2161 cccggatcga ggcgtcaagc tggtagcgcc cgtccgcgaa cagccggacg tcgagccggt
     2221 cggcgccgga ctggctgcgc aggggcagca gcaccgcctg gccgtcgcag cgggcctcgc
     2281 cgctgaagcc ggggaccagc ggcgcggcgc cgagctggcc cgccagccgc accttgacca
     2341 tgccttcggc ggcggcgcac tggccgtcgg cgaagcgggc gctgacgtcg ctgaggtcga
     2401 gagtggccgg cggcaccgcc ccgaagccgg cgacctcgat cgtcccggaa gcgtcgtcga
     2461 tgccgaagcc gtgacgcgag gcggtgagac cggcgcggaa cccgtcgcct tcctgggcga
     2521 cgtcgagcct ggcgcgcccg gcgagcagcg gcagcactcg aagccgcgtc gccaccgttc
     2581 cgagcgggac cgggcccagc tgcgcctcgt cgagcgcgcc gacccagagg ctgccggccg
     2641 cgcttcgagc ggtcaggccc ctttccgcaa agccgaggct gtcgacggcg aagcggagcg
     2701 ggagcagcgc cacgagagcg aacagcaggg cgccgaggaa gaacagctcc cgccaccagc
     2761 gcagccgcag gcgcatcagc ggcccctcgc cctgagcacg atctgggcgg agagggtccg
     2821 gtccggattg ctggtcgcgc tcagccgctc cacgacatag ccctgcccct cgagtgcgga
     2881 aatccagctg aagagcggct gcggtcgagc cgcgccgagg gcgaaggaga ctcggccggg
     2941 tccctgcggc tgcagcgccg agagctggaa gcccgcctcc gatgccgccg ctgcgacggc
     3001 ctggtcgatc gacccctcca tgaccgcggg gccgccgcgc tcgagcatcg cgatctcggc
     3061 cgctcgccgg cgggtctcgg ccagcgtcgc gacggcatcg ccatgccgct gcctcgccgc
     3121 cgacagcatg tcgcccagcg gccggacgac gaggaaccag acgagcacga gggcgccgag
     3181 cgcgagcatc accgcgagca gccagcgctc gcgcggagtg cgcgccagcc agagcgcctt
     3241 caggcgggcg ctcatcgcgg ccgcaccatc aggtcggcga ctcggcgccc gccgcccgga
     3301 cgcggcgctc cgacctcggc ggcgaagccg ttggcctcga cccgctggct caacgcctcg
     3361 atcgccgccg gcgaatcggc ctgaaccgtc gcccgcagaa ggccgtccgg cgagaatgcc
     3421 agcgcggaca gctcgacatt gggcgtggcc ttgaccgcgt cgaacagggc ccccgccgtg
     3481 gcgccgaagc cggcgccgcc gccgcgcagc tccgccagcc gctgggtgag ggccgcttcg
     3541 ggatcggcga cgcccgggct gcgcggcagc gccgcggcgg cgacccgccg ggtctcggcc
     3601 tcggcggcgt cggccgcgaa gatgtagcgg gcgatcgccg cgacctggac ggcgaggctg
     3661 acgagcagga acgccgcggc cagcaccgcc agccgccgca tccgagccgg cgccacccgc
     3721 cagtcgcggc ggcgcgcgaa cggaccctgg cgaaggtcga gcggagcctc ggcaagcgcc
     3781 tcggccagcc ccgcctcgcc gtcgaggagg acggcgtcgc ggccggccag caggagctcg
     3841 ccgagctcgg gctcgacggc gaaggcctcg tcgcgcgccc ggtagagccg caggggtccg
     3901 gagtcgcgaa cgtggaaccc ctcctccggc gcgggcagca gcagcgtctc ggggatgatt
     3961 cgctcggcct cgaagccggc ggcctcgacg ctcgccagcc aggcgcgcat cgtctccatc
     4021 ggcaccaggc cgacgcagcg tgcgccgtcc caggtctcgc gcccggccgc gacgtggagc
     4081 tcgtcgagcg gctggccggc gagctcgccg gccatgagcc tcgccgcggc ggcggcctgg
     4141 gccggcgaca ggcccgccgg caggtccagc cagcgcaggg tcacctcctc gcccggcgcc
     4201 accgccaccg ccggcgcgcc gcgatgggcc tcgctcccgt cgatgccggc gccgcgcgcc
     4261 gcaaccgcgc cgtcggcgat ccggagccag ccctcgacgc cgccgccgcg cgcgaggaag
     4321 aggagcagcg cgtcgttcac tcgcttccgc cccatcgccg cgccaccacc ttggccggcg
     4381 ccagccgggc gtcgaccagc gcccattcct ccatctccgc gccctggaag gcgaccctga
     4441 ggtcgatggt gaaccaatag gtcttgagtt ggagctggga catcggatcg gcgttggtct
     4501 ctagcccggc ctgctccgcg cttcgccaga aatcgtacgt gttgcgccag ccggccggcg
     4561 gccgcgccgc gatcagcccg cgcgcccggt cgaccctcgt cttggtgggc gcgaacatgg
     4621 cgagcagggg cgcgtcgcgc ggcagcaggg tgttgacgtt gatcggcgac atgtccgcgg
     4681 ccgggagagc gcacagatag ggcctgagct ggcggtagag ttccggcgtc accccggcga
     4741 cggcgcgaag ctcgctcggg tccgcgagca gggtgttgcc ggcccggtag gcgggctcgc
     4801 cgccggcata ggccgaatcc tcggcgccct ggggccccgt ctccgaatcg ctgtcgatcc
     4861 agtcggctga ggcggccgcg acccggcgcg cggacggctc cggcagcccg aggacgagca
     4921 tcaggccgac gaactgctcg atcgccgccg gccgctggac cagcgtggtg gcgacgtcgc
     4981 cctgcaccag gctgttgagg ttgaagcaat tgccgccgtc gcggatcgtc ccgatcgcca
     5041 gcccctcccc ggggagcggg attcgccgcg ccgttccgtt ccagtcgccg gccaatgtgg
     5101 tgacataggc gtcctcgccg gcgagatcgt cgatcctcag cgccagcagg tcctcgactc
     5161 cgagcgcgta ggcgcgcgcc tggtcgagcg cggcgccgtt catcgccatc gccgtcgaca
     5221 gccgaagccg ctcgaacgcg ccggccgcga tcgccgacat gatcacgacc agcaccagca
     5281 ccgcgagcag ggcggcgccg cgctccgacg cggggacggg ccggttcacc ctgacgtgcc
     5341 cgtctggaag agctggcggg tggtgccgct gccctcggca tcgagcacca gctcgaccgc
     5401 gcgcggcagc tcgctcgcct tggtcgggtc ccagcgctcg cgccaattgc cctcgccgtc
     5461 gcgatagcgg agccggacgc ggcggacgcc gtcgaccacg gtcgtcgccg gaaacggcgc
     5521 cgcgccgtcg agccgggggt agatccggcg ctcgagccgc ccgtcgacga cgctgtactc
     5581 gacccgctgc agcgacgcgc gcggcgcctc gtccagattc tcccagccgc gccgaacgaa
     5641 ggcgagcgcg actccgccct gctcgccggt gccgccgtgg aaggccgggc gcgaattgcc
     5701 cgaggagtcg cgcgccagcc gcggcgccgc ctgggcgagg tcgccggcga gcagggctcc
     5761 agcgcgccgg aagtcggcga gatcgccgag ccgggcctcg gccgcctcct gcgcccggac
     5821 gctgaacgag agcaggccga cgcccgcggc cgcgagcagc ccgaagatga acagcgagac
     5881 gagcagctcg accagcgtga agccgccgtc gctcctcccc ggaacgggga gggggaccgc
     5941 cgccgaaggc ggtggtggtg gggtcccggc ctcggcgcag cgcagaggtg ggccccctcc
     6001 accctgcttc gcagggtccc cctccccgtt ccggggagga atttggagcg cgaccctcat
     6061 cgcgggcccc ttcggaacag ggtcaggttc gccgcttccg ggccgcggtc gctgcgcacc
     6121 tcgatccgga tctgctggat ccggggctcg ggcgagcggc cgacggtccg cagccagcgc
     6181 catttgcgcc ccgcattgat cgcctcgccg cgcgtcgcgc cgaaggccgg cggccgggga
     6241 tcggtcaggg tctcgaccgc cagattgcgc gccaccacct gggcgagcgc ctgctcccgg
     6301 agcagggcgg tggtcgagac cgtcgcgcct tcaagcctca gcagggccag ggcggcgagg
     6361 ctgaacacgg cgagcgcgac cagcatctcg atcagcgtga agcccgcctc gtcaggggag
     6421 gacatggacc cgtcccccgt ccgacacctc gaccaccgcc tcctcgcggc cccgcaccag
     6481 gcgaagccgc gccggctcgg cgaatccggt cgaatcgaac acgacccgcg cttcgccgcc
     6541 ctcgatcgaa gcccgcgtcc cctcgctcca gtcctgcgcg acgaacggcc gctgcgcgat
     6601 cggccgccac tcgccttcgg tgcgccattc gaagccgtag ccgccttcgc cgagcacgag
     6661 cgcgaccggg cggttgtcca tcatcgcccg ctcgcgcgcg gcgtcggtgc gggcggccag
     6721 ccggagcgcc tcctggcgaa gcgccccgcg cgggtctggc atcgccagca ccaccgccgc
     6781 cgagacgagc ccgacgatga cgaggacgac catcagctcg acgagggtga agcctccgtt
     6841 gctcctcccc ggaacgggga ggggaaccat gcggagcatg gtggaggggg cccagcgtgg
     6901 gcactgcgaa gacgtgggcc ccctccacca ccgccttcgg cggcggtccc cctccccgtc
     6961 ccggggagga accagggcag cctttccact caaccctcag ctgtcccagt tgccgatgtc
     7021 cgcatcctgg ccctggcccc cttcgcgccc gtcggcaccg tagctgtaga tgtcgaacgc
     7081 ccgaccgtcg cggcccggcg cggcgtagcg gtagggattg ccccagggat ccttgggcag
     7141 gcgcttgaca tagccttccg aagacaccgc ctgcagccct tccgacgcgc tcggatagcg
     7201 aagcttggtc aggcggtaca tctcgacccc ctgctcgagc gtggcgacgt cggccttcgc
     7261 cttggtgatc gcggccttgt ccgtcgccgg cagcacgttg atgatgacga cggtcgcgag
     7321 cagtccgatg atgacgatga cgaccatcag ctcgaccagg gtgaagccct tctcattcct
     7381 ccccggcctt tgccggggag gaggaccatg cgaagcatgg tggaggggtt cttgcgaccc
     7441 cgaagaaccc ctccaccgcg ctccgcgcgg tccccctccc cgcgaaaacg cggggaggaa
     7501 tttgccacga taatccttca tcccattcct ccggcaagtg tgtcgagctg caggatcggc
     7561 agcaggatcg aaagcacgat gaccgcgacc agcgcgccca tcaggacgat gatcgccggc
     7621 tcgagcagcg cgagcgcggt cgtggtgaag ctgtcgaact cgcgctcgag atagtcggcg
     7681 gcccgctcca gcatcaggtc gagccgcccc cccgcctcgc cgctcgccgc caggtagacg
     7741 agcaggggcg ggaagatgcc cgagcgcttg agggctccgg agaggctgcc gccggtgcgc
     7801 accgcctcgg cgatctgcgc ggtggcggcg cgaagggcgc ggttgtggac cgtctcggtg
     7861 gtcagcttca gcccctcgag cagcggcagc cggctcgcca ccatggtcga gagcgtgcgc
     7921 gccatccgag cggcgtggag gtcgcgcatc agccggccga tcaccggcac gcgcagcagc
     7981 gcacggtcga agcgaagccg gagccgctcg tcgcgcagcg cccggctcgc cgcgaatccg
     8041 atcagcacca gcccgaggag cagcgcccac caccagcccg ccagcaggtg cgagaccgcg
     8101 atcacggcgc gggtgaggaa aggaagctgc tggccgatat cctcgaactg ctcgaccacc
     8161 cgcggcacca cgaacaccat cagcgcgaag acgacgaaag cggcgacgat cgccagcacc
     8221 gccggatagg cgagcgcgct cagcaccttg cttcgcacct ggccctgccg ctcgagcagc
     8281 ccggcgagcc gctcgaggat ggcgggcagg gtgcccgacc cctcgcccgc ggccaccatg
     8341 ccccggtaga gcggcgggaa gctggccggc tcgcgcgcca tcgcctcgga caggcgccgt
     8401 ccctccagca ccgagccgtg gaccgcgagg aggacgtcgc ggacctggtc cttctcggtc
     8461 tggcgagcga tcgtcctcag cgcctcctcg agcgggctga cctggaccag cgtcgacagc
     8521 tgccgggtga agacggtgag ctggcgggcg ttgagcttct ttcggacgaa cagcttgcgc
     8581 gacagcaggg ccggagccgc ctcgccgctc gcgctctcca tcctgacgac gaacaggccc
     8641 cgcgcgctca gcttctcgcg cgcctcgtcg ggcgtcgcgg cccgaagcga tcctcgccgc
     8701 tcgcggccgg cccggtcgag gaccagatag gcgaattcag acatcttcgc tctcgcggcg
     8761 cgagatccgc accgcctcgc cgacgcttgt gatcccctct cggaccagct cgcgcgccgc
     8821 ggccgccagg ttgggcgcgt tgcggaaggc atgggcggcc atctcgacct cgtcgccgcc
     8881 ggcgttgatc agccggcgca cattgtcgtc gacccgcacc gcctcgaaca cgcccgtgcg
     8941 gcccttgaag ccgctgttcc cgcattcggc gcagccgcgc ggctcgtgga cgatcgtgcc
     9001 cgcgggaagc ccgatcatgg cggccgtcgc cgcgtcggcg gcgaccggcg cccggcaggc
     9061 cgggcacagc cgccgcacca gccgctgggc gatcacggcc cgcaaggtcg aggcgagcag
     9121 gaaaggctcg accttcatgt cgcgcatccg ggtgatggcg ccgatcgcgt cgttggtgtg
     9181 gacggtcgag aggacgaggt ggccggtcag cgacgcctgg accgcgatct cggcggtctc
     9241 ccggtcgcgg atctcgccga ccatgacgac gtccggatcc tgccgaagga tcgcgcgcag
     9301 gcctgcggcg aagctgaggc cgaccttggg attgacctgg gtctggccga ctccgtccac
     9361 cgcatattcg accggatcct cgacggtgag gatgttgcgg gagccgtcgt tgagcagccg
     9421 cagcccggca tagaggctgg tcgtcttgcc cgagccggtc ggtccggtga cgaggacgat
     9481 gccgttgggc tcggcgagcg cgtcgcgcag caggcggtgc acggaaggcg gcattccgag
     9541 cgaatcgagg tcgatgccgg cattctcctt gtcgaggatg cgcagcacga cccgctcgcc
     9601 ggcgcggctg ggcagcgtcg agacgcgcac gtcgagcagc ttgccgccca gcgtcagtcc
     9661 gatcctgccg tcctgcggca cccggcgctc ggcgatgtcg aggcgcgcca tcaccttgat
     9721 ccggctgacg acgaccggcg ccacatgggc cggcatccgc agggtctcgc gaagcacccc
     9781 gtcgatcctc atccggacca ccagcccggt ctcgtagggc tcgatatgga tgtcggacac
     9841 gccctgccgc gcggcgtcgg cgatgatgcc gttgatgagc cggatggcgg gcgcgtcgtc
     9901 ggcgctgtcg agaaggtcgt cggcggtggg caggtcggtg gcgaggtggc tgagctcgtc
     9961 gcccaggccg agcgatccgg ccgcgtcggc cgccgcctgc tcgtccatcg cgtagcgctc
    10021 ggacaggagc ttgtcgaagc cttcggcggt ggcggattcg accgcgagcg gccggcccag
    10081 gacccgccgc gcctcgatga gggcgcgcgg gtcggcgccc tgcctcaatg cgacgcgcgc
    10141 ctggccgttc gcctccccga cgagagcgac gccattgcgc ttggcgaaca gataggggag
    10201 attgggtgca ggcgcgtcgc cccggcgaaa gccgggcccc ggcggcgaag gctcctgccc
    10261 ttcctggccc gggtcccggc tttcgccggg atgacggggg gcgttcaccg cggcgtgccc
    10321 gccggcggcg cggcgggcgg cgtgaccacc gcatcgcccg
//
LOCUS       DASVQZ010000394         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_372131, whole genome shotgun sequence.
ACCESSION   DASVQZ010000394 DASVQZ010000000
VERSION     DASVQZ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372131"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VF547_11125"
     CDS             <1..1520
                     /locus_tag="VF547_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8263414.1"
                     /translation="SGEATGAAEADLLIRGGTVYTGSAPPFVGDVAIAGDRLLAVGPS
                     LDRPARRVIAAKGMIVAPGFIDPHTHVGEELASADARARLVPAFLMQGVTTAFIGNDG
                     GGDPDVAAVLGRAEARPVGINYAAYVGFGAVRERVIGRADRAPSAEELRRMRGLVADA
                     MCQGALGLSTGLFYAPQSFAATGEVIAVALEAGRRGGVYDSHIRDESSYTIGLAAAVD
                     EAIAIGREARLPVNISHIKALGVDVRGQAPAIAAKVEAARRAGQAVTADQYPWSASGT
                     TLAASLVPAWAQDGGRSELLKRFDDPGLAPRLRAEMAENLRRRGGAAALRITEGRSRG
                     RTLADLARAQGGDSIAAAIAVIRSGDPAVASFNQAEADIAQFMRRPWVMTGSDASKGH
                     PRAYGSFARKYAKYVVADRVISLRQFIERSSTLAADTFGLTGRGRLQPGAFADLVVFD
                     PRRYAARATYDRPTLPAAGVRTVVVNGVVAVDKGALTGRAAGRALRHRPAPGSCP"
     gene            1517..2347
                     /locus_tag="VF547_11130"
     CDS             1517..2347
                     /locus_tag="VF547_11130"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8263415.1"
                     /translation="MMPEHAARFRRDGYAVVRGLFGPDEVAAIAAAADRVHAEAVAHG
                     RSFRHGNLFYNVAPDSAGAPQVRMVQWPSYHQPVLNAVRLDRRLATLLEPLLGRDLKQ
                     IFNQIHWKEPGGPSEFAWHQDCQFREPAHAYRNLDRSYVQTGLAIDPHTPESGCMRFI
                     PRSHVDGDLHLATPREVLGVAMNDSVLHSAGLDPGSAVEVELEPGDLALWGPFLVHGS
                     GVNRSDHQRRLYVNGYVRAQDCDRGEWAFRDGEPVPFGPEPALVHYEALRERGGPHYV
                     "
     gene            complement(2388..4436)
                     /locus_tag="VF547_11135"
     CDS             complement(2388..4436)
                     /locus_tag="VF547_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8263416.1"
                     /translation="MGWRWCVHLGLLAAVLALRPALASAPAKSDPLAAALALPLAGEL
                     VGARDAPRFAWVEKAAGVRNVWIADAGRPARQVTAYGEDDGQDIYDLALSPDGARLAF
                     VRGGDPDSRDDPPPNAGSAAAPPRQQLFVAGPEGAEPRPVGEGHSPAFSPDGTRLAFA
                     RRGEIWLWQAGGEARKLASNLAEVRRLQWSPDGTRLLFVSHRGEYSFVALLDPEASRL
                     VYLDPGLAFSVEPVFSPDGRKVAFIRHVDAPAGAAPDSGPYWSVRVADVASGAARQLW
                     AAPPGAGARYYESRGRDLFWSEDGQLIFPWERSGWLHPYAIDSAEGGEPRDLTPGAFE
                     VEMFMLGAGGRSLVWAGNEGDLDRRHIWRRPLAGGPAERLTRGNGIETAPALAGNRLA
                     VIATDVVHPAHVALVDAGLRPLKPVPAAKNFAAPEAVLLRAADGLEVHGQLFRARGKG
                     PHPAIVYVHGGPRRQMLLGFHPSAYYSNAYVLNQHLAALGYHVLAVNYRSGTGYGNAF
                     RDAPGLARGGASEYRDVLAAGRWLAARADVDAKRIGIWGGSWGGYLAALGLARDSDLF
                     AAGVDFHGVHSMLRPVPNSLSPDAQAAARQLQWDSSPLGAIDRWRSPVLLVHGDDDRN
                     VDFSQSLLLARELTARRIPFRELVFPGERHSFLLHASWLASFRAAEAFLEETLKTPRR
                     "
BASE COUNT          632 a         1694 c         1632 g          570 t
ORIGIN      
        1 cttccggcga ggcgaccggc gcggcggaag cggacctgct gatccggggc ggcaccgtct
       61 ataccggctc ggcgccgccc ttcgtcggcg acgtcgcgat cgccggggac cggctccttg
      121 cggtcggacc gagtctcgac cgccccgccc ggcgggtcat cgccgccaag gggatgatcg
      181 tcgcgcccgg cttcatcgac ccgcacaccc atgtcgggga agagctggcc tccgccgacg
      241 cccgcgcccg cctcgtcccc gccttcctga tgcagggcgt gacgaccgcc ttcatcggca
      301 acgacggcgg cggcgatccc gacgtcgcgg cggtgctcgg ccgggccgag gcgaggccgg
      361 tcgggatcaa ctatgccgcc tatgtcgggt tcggcgcggt ccgggagcgc gtgatcggcc
      421 gcgccgatag agcgccctcc gccgaggagc tgcggcggat gcgcggcctc gtcgccgacg
      481 ccatgtgcca gggcgcgctc ggcctttcga ccggcctgtt ctacgcgccc cagagcttcg
      541 ccgcgactgg cgaggtgatc gccgtcgcgc tcgaggcggg gcggcggggc ggggtctacg
      601 acagccacat acgcgacgaa tcctcctaca cgatcggact cgccgccgcc gtcgacgaag
      661 ccatcgcgat cggccgggag gcccgccttc cggtcaacat ctcccatatc aaggcgctcg
      721 gcgtcgacgt caggggccag gcgccggcga tcgcggccaa ggtggaggcc gcccgccggg
      781 ccggccaggc ggtgaccgcc gaccaatatc cctggtccgc gtccggcacg accctcgccg
      841 cctcgctcgt cccggcttgg gcccaggacg gcggccgaag cgagcttctc aagcgcttcg
      901 acgatcccgg cctcgccccc cggctgcgcg cggagatggc cgaaaacctg cgtcggcggg
      961 gcggcgcggc cgcgctccgg atcaccgagg gccggtccag gggacggacg ctcgccgacc
     1021 tcgcccgggc gcagggcggc gactcgatcg ccgccgccat cgccgtcatc cgctccggcg
     1081 acccggcggt cgcctcgttc aaccaggcgg aggcggacat cgcgcagttc atgcgccggc
     1141 cctgggtgat gaccggctcg gacgcctcga agggccatcc gcgcgcctac ggcagcttcg
     1201 cgcgcaaata cgccaaatat gtcgtggcgg accgggtgat cagcctgcgg cagttcatcg
     1261 agcgcagctc gacgctcgcc gccgacacgt tcggcctgac cgggcgcgga cgactgcagc
     1321 ccggcgcctt cgccgacctc gtcgtgttcg atccccggcg ctacgccgcc cgcgccacct
     1381 acgatcggcc gacgctgccc gcggccggag tccggaccgt ggtcgtcaac ggcgtcgtcg
     1441 cggtcgacaa aggggccctg accggccgcg ccgccggccg ggcgctccgg caccggccgg
     1501 ctccgggatc ctgtccatga tgccggaaca tgcggcccgg ttccggcgcg acggctatgc
     1561 cgtcgtccgc ggcctgttcg ggccggacga ggtggcggcc atcgccgccg ccgccgaccg
     1621 cgtccacgcc gaggcggtgg cgcacggccg cagcttccgg cacgggaacc tcttctacaa
     1681 cgtcgccccg gactcggccg gcgcgccgca ggtgaggatg gtgcaatggc cgtcctatca
     1741 ccagcccgtc ctcaacgcgg tccgcctcga ccgccgcctg gcgacgcttc tcgagccgct
     1801 cctcggccgc gacctgaagc agatcttcaa ccagatccac tggaaggagc cgggcggtcc
     1861 aagcgagttc gcctggcatc aggactgcca gttccgcgag ccggctcacg cctaccgcaa
     1921 cctcgaccgc tcctacgtcc agaccggcct ggcgatcgat ccccacacgc cggagtccgg
     1981 ctgcatgcgc ttcatccccc gaagccatgt cgacggggac ctccacctgg cgaccccgcg
     2041 cgaggtgctc ggagtcgcga tgaacgactc cgtcctccac tcggcgggcc tcgatcccgg
     2101 gagcgcggtc gaggtcgagc tcgagccggg cgatctggcg ctgtggggtc cgttcctggt
     2161 gcacggctcc ggggtcaacc gctcggacca tcagcggcgg ctctacgtca acggctatgt
     2221 gcgggcccag gattgcgacc gcggcgaatg ggccttccgc gacggcgagc cggtcccgtt
     2281 cggccccgag ccggcgctgg tccattacga ggccctgcgc gagcgcggcg gtccccacta
     2341 cgtctagggc tcgcggtgga agcgcagcag gccggcccga agccgcttca ccggcgcggc
     2401 gtcttcagcg tctcctccag gaaggcttcg gcggcgcgga agctcgccag ccagctggcg
     2461 tgcaggagga aggaatggcg ctcgccggga aagaccagct cgcggaacgg gatccggcgc
     2521 gcggtcagct cgcgggccag cagcagcgac tgggagaagt cgacgttgcg gtcgtcgtcg
     2581 ccgtggacga gcagcaccgg cgaccgccag cggtcgatcg cgcccagcgg agaggaatcc
     2641 cactgcagct ggcgggccgc ggcctgcgcg tccggcgaca gcgagttggg gaccggccgg
     2701 agcatcgaat ggacgccatg gaaatcgact cccgcggcga acaggtcgct gtcccgagcc
     2761 aggccgaggg cggcgagata cccgccccag ctgccgcccc agatgccgat ccgcttcgca
     2821 tcgacgtcgg cgcgggcggc gagccagcgc ccggcggcga gcacgtcgcg atattccgac
     2881 gcgccgccgc gggcgaggcc gggggcgtcg cggaaggcgt tgccgtagcc ggtgccgctt
     2941 cgataattga ccgccagcac gtgatagccg agcgccgcca gatgctggtt cagcacatag
     3001 gcgttcgaat aataagccga cgggtggaag ccgagcagca tctgccggcg cggcccgcca
     3061 tggacgtaga cgatggccgg atgcggcccc ttgccccggg cccggaacag ctggccgtgc
     3121 acctccaggc cgtcggcggc gcgcaacagc accgcctcgg gcgcggcgaa gttcttcgct
     3181 gccgggacgg gcttgagcgg ccgcagcccg gcgtcgacca gggcgacgtg ggccggatgg
     3241 accacgtcgg tcgcgatcac ggcgagcctg tttcccgcca gggccggcgc ggtctcgatg
     3301 ccgttgccgc gggtcaggcg ctcggcgggg ccgccggcga ggggccgacg ccagatgtgc
     3361 cggcgatcca ggtcgccttc attgccggcc cagacgagcg agcggccgcc cgcgccgagc
     3421 atgaacatct cgacctcgaa agcgcccggc gtcaggtccc gcggctcgcc gccctcggcc
     3481 gagtcgatcg cataggggtg cagccagccg ctgcgttccc agggaaatat gagctggccg
     3541 tcctcgctcc agaacaggtc gcggccgcgc gattcgtaat agcgggcgcc ggcgcccggc
     3601 ggcgccgccc acagctgtcg cgccgccccg ctcgcgacgt cggccacccg gaccgaccaa
     3661 taggggccgc tgtccggggc cgctcccgcc ggggcgtcga cgtgccggat gaacgcgacc
     3721 ttgcgtccgt cgggggagaa gaccggctcg accgagaagg cgaggcccgg atccagatag
     3781 acgaggcggg acgcctcggg gtcgagcagg gcgacgaagc tgtactcgcc gcgatggctg
     3841 acgaagagca ggcgggtccc gtccggcgac cattgcaacc gcctgacctc ggcgaggttc
     3901 gaggcgagct tgcgcgcctc gccgcccgcc tgccacagcc agatctcgcc gcgccgcgcg
     3961 aaagcgaggc gcgtgccgtc gggcgagaag gcgggcgaat ggccctcgcc gaccggccgc
     4021 ggctcggcgc cttccggtcc ggccacgaac agctgctgcc ggggcggcgc cgcggcggat
     4081 ccggcattgg gcggcgggtc gtcgcgggag tccggatcgc cgccgcgaac gaaggcgagc
     4141 cgggccccgt ccgggctcag cgcgaggtcg tagatgtcct ggccgtcatc ctcgccatag
     4201 gcggtcacct gccgcgccgg ccggccggcg tcggcgatcc agacgttgcg gacccccgcc
     4261 gctttctcga cccaggcgaa gcgcggcgcg tcgcgcgcgc cgacgagctc gccggccagc
     4321 gggagcgcca gcgccgccgc cagcggatcc gacttcgccg gcgccgaagc gagagccggt
     4381 cgcagcgcca gcacggccgc cagcagcccc agatgaacgc accatcgcca ccccatcgcc
     4441 tcctattgaa gcgagcccga gcccgcgaca agcgcgcagg gccgccgcca tgcgcccggg
     4501 ttatggccgc tcgaagggtc tgaatgat
//
LOCUS       DASVQZ010000395         4779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_469834, whole genome shotgun sequence.
ACCESSION   DASVQZ010000395 DASVQZ010000000
VERSION     DASVQZ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469834"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VF547_11140"
     CDS             <1..764
                     /locus_tag="VF547_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263417.1"
                     /translation="NPPVLPTIQGAILLCDGENGALLAVLDSIEVTLRRTAAATALAA
                     RLLARPGSQTLLVCGCGAQAAAQVEALREVLPLRRCFTWDRDRGRAEAFAEEAARTHG
                     LEAGAAASLAEAAAASDVIVACTTAREPFLLSGHVRPGSFVAAVGADSPEKGEVHPEL
                     MGRTWVVVDSLDQCLAMGDLRHAVGAGTMAARDVHAELGAIVVGTAPGRISDDQIFIF
                     DSTGTALQDVASAALVYERACRRGGYSQFEFGGAA"
     gene            761..2113
                     /gene="chrA"
                     /locus_tag="VF547_11145"
     CDS             761..2113
                     /gene="chrA"
                     /locus_tag="VF547_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX8263418.1"
                     /translation="MTAPRQPVTLAEATRVWAKIALLSFGGPAGQIALMHRILVEERK
                     WIGEARFLHALNYCMLLPGPEAQQLATYIGWLMHRTRGGLIAGILFVLPGMVAIMILS
                     WIYAAAGSVGAVEALFFGLKAAVLAIVAEAVRRIGARALRNNVLRAVAAAAFVAIFFF
                     AVPFPLIVLAAGAAGYLAARAGSAAFGVGGGHGPAAPDDSLLGDRIPDHAVPSLRWSL
                     RVSAIFLLLWLAPVALLLAALGPDHVFSRIALFFSQMAVVTFGGAYAVLAYVAQQAVE
                     TYGWLAPGEMLDGLGMAETTPGPLIMVTQFVGFMGAWRNPGGLDPMLAGTLGGLLATW
                     VTFTPCFLWIFLGAPFVEKLRSNRALTAALTAITAAVVGVVLNLAIWFALHSLFREVR
                     EVNSGPLRFDLPTPASIDLAACALAAAALLAMFRFRLGAPALLAGCAAAGMAWWALGG
                     "
     gene            complement(2463..3632)
                     /locus_tag="VF547_11150"
     CDS             complement(2463..3632)
                     /locus_tag="VF547_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8263419.1"
                     /translation="MIAEREALLDTGVRDPFGLVMEEVLSPTRAIVRGKETILLGTYN
                     YMGMTFDPDVVDAGKRALDEYGSGTTGSRVLNGTYQGHKECEEALKDFYGTRHAMVFS
                     TGYQANLGLMSTIASKEDFIILDADSHASIYDGCALGSAQIVRFRHNSVEDLDKRLGR
                     LPAEGGRLVVLEGVYSMLGDIAPLAEMVRIAKKHGAMVAVDEAHGMGFFGEHGRGVFE
                     EAGVEDQVDFVVGTFSKSVGTVGGFIVSNHPKFEVLRLVCRPYVFTASLPPSVVATAA
                     TSIRKLKYAGNKRQHLWENSRRLHKGLRDLGFKLATPEAQSAIIAILLPDQDTTVRTW
                     QALLEAGVYVNMARPPATPAGMYLLRCSLCADHSEEQVGQILAMFEAAGRATGVL"
     gene            complement(3868..4116)
                     /locus_tag="VF547_11155"
     CDS             complement(3868..4116)
                     /locus_tag="VF547_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8263420.1"
                     /translation="MTDREETFGKVATLIEPFNKKGVELTDATTFAGDLEWDSLVVLD
                     FVASVEDEFDILITMNMQAEIETVGQLVDAVEELRNDG"
     gene            complement(4172..>4779)
                     /locus_tag="VF547_11160"
     CDS             complement(4172..>4779)
                     /locus_tag="VF547_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX8263421.1"
                     /translation="RQDGETARVRLDVPGLHNVTNALAALAAAGAAGLPLAEAAEALA
                     GFEGLRRRFDLVGTRNGITVIDDFGHNPDKIAATLAACRAAPGRLLIFFQPHGYGPLR
                     TMRSELVAAFAGNMAAEDVLLMPDPVYYGGTVERVVTGADIVGDVRAAGRTAEHVGDR
                     SEAGRRLAELARPGDRILVMGARDDSLSQFAHDLLGALPPA"
BASE COUNT          628 a         1604 c         1751 g          796 t
ORIGIN      
        1 ccaatccgcc cgtcttgccg accatccagg gcgcgatcct gctgtgcgac ggggagaatg
       61 gcgccttgct ggccgtgctg gactcgatcg aggtcacgct tcggcggacg gcggcggcga
      121 cggcgctcgc cgcccggctg ctggctcggc cgggctcgca gaccctgctg gtctgcggct
      181 gcggcgccca ggcggcggca caggtggagg cgctccgcga ggtcctgccg ctgcgccgct
      241 gcttcacctg ggaccgggat cggggccggg ccgaggcctt cgccgaggag gccgcgcgga
      301 cgcacggcct cgaagcgggc gcggcggcgt cgctcgccga ggcggcggcg gcgagcgacg
      361 tcatcgtcgc ctgcaccacc gcccgcgagc cgttcctcct gtccggccat gtgcggccgg
      421 ggagcttcgt cgccgcggtc ggggcggaca gcccggagaa gggcgaggtc catcccgagc
      481 tgatgggccg gacctgggtg gtcgtcgaca gcctcgatca atgcctcgcc atgggcgacc
      541 tccgccacgc cgttggtgcg gggacgatgg cggcgcggga cgtccatgcg gaactgggcg
      601 cgatcgtcgt cggcacggcg ccgggacgga tctcggacga ccagatattc atcttcgact
      661 cgaccggcac cgcgctccag gacgtggcca gcgccgccct ggtctacgag cgcgcctgcc
      721 gccgcggcgg ctattcgcag ttcgagttcg ggggagcggc gtgaccgcgc cccggcagcc
      781 ggtcacgctc gccgaggcga cccgggtctg ggcgaagatc gcgctgctca gcttcggcgg
      841 gccggccggc cagatcgctc tgatgcaccg gatcctcgtc gaggagagga aatggatcgg
      901 cgaggcgcgc ttcctccacg cgctcaacta ctgcatgctc ctgccaggcc ccgaggcgca
      961 gcagctcgcc acctatatcg gctggctgat gcaccggacc aggggcggtc tgatcgcggg
     1021 catcctgttc gtcctgccgg gcatggtcgc gatcatgatc ctgtcctgga tctatgcggc
     1081 ggccggaagc gtcggcgcgg tcgaggccct gttcttcggg ctcaaggcgg ccgtcctcgc
     1141 catcgtcgcc gaggcggtgc gaaggatcgg cgcccgggcg ctccgcaaca atgtccttcg
     1201 agccgtcgcc gccgcggcct tcgtcgccat cttcttcttc gcggtgccgt tcccgttgat
     1261 tgtgctggcg gccggggcgg ccggctacct ggccgcccgg gcgggctcgg cggcgttcgg
     1321 agtcggcggc gggcacgggc cggccgcccc cgacgacagc ctgctgggcg accgcattcc
     1381 cgaccacgcc gtgccgagcc tgcgctggtc gcttcgggtc tcggcgatct tcctgctgct
     1441 gtggctcgct ccggtcgccc tgctgctggc ggctctgggt cccgaccatg tgttcagccg
     1501 catcgccttg ttcttctcgc agatggcggt ggtgacgttc ggcggcgcct atgcggtgct
     1561 cgcctatgtc gcccagcagg cggtggagac ctacggctgg ctcgccccgg gcgagatgct
     1621 cgacgggctc gggatggcgg agaccacgcc ggggccgctg atcatggtca cccagttcgt
     1681 cggcttcatg ggcgcctggc gcaatcccgg cggcctcgat ccgatgctgg ccggaacgct
     1741 cggcggcctg ctcgccacct gggtgacgtt caccccctgc ttcctgtgga tcttcctcgg
     1801 cgcgccgttc gtcgagaagc tccgctccaa ccgcgcgctg accgcggcgc tgacggcgat
     1861 caccgccgcg gtggtcggag tcgttctcaa cctcgccatc tggttcgcgc tgcacagcct
     1921 gttccgcgag gtccgggagg tgaattcggg cccgctccgc ttcgacctgc cgacgcccgc
     1981 cagcatcgac ctcgccgcct gcgccctcgc cgccgccgcc ctgctggcga tgttccgctt
     2041 caggctcggc gctcccgcgc ttctggccgg atgcgcggct gcgggtatgg cctggtgggc
     2101 gctcggcggg tgaagtgacg gtcaccctcc gggaagggcg cttccatgat catcctgttc
     2161 gtggtatgtt ctagcacggc tgcttgcctc ggcgggtgag cagcggagcg gcggcaagga
     2221 agctgggggt gatggtcgga gacagaccgt tccgggtccg gagtgcgacg gcgccaagcc
     2281 cttggccggc aatgattctg gccgcgaagt tcattcgaaa cgcgcccgac ttgatgaact
     2341 tcatgaactt cgccggaaaa acgcggtgtt ttcagcactc tcgcagttgg aagaaagcgc
     2401 tcgggccgga ccgcggaggt ccgttcgagg ccgtcggccc gaacgaagcc cgcttgctgc
     2461 cgctacagga cccccgtcgc cctccccgcg gcctcgaaca tggcgaggat ctggccgacc
     2521 tgctcctcgc tgtggtcggc gcagagcgag cagcgcagca ggtacatgcc ggccggagtg
     2581 gcgggcgggc gggccatgtt gacatagact ccggcctcga ggagggcctg ccaggtccgc
     2641 accgtcgtgt cctggtcggg caggaggatg gcgatgatcg ccgactgggc ctcgggcgtg
     2701 gccagcttga agccgagatc cctcaggccc ttgtggagcc ggcggctgtt ctcccagagg
     2761 tgctggcgct tgttgccggc atatttgagc ttgcggatcg aggtggcggc ggtggcgacg
     2821 acgctcggcg gcagcgaggc ggtgaagacg taggggcggc agacgagccg gagcacctcg
     2881 aacttggggt ggttggagac gatgaagccg ccgaccgtcc cgaccgactt ggagaaggtg
     2941 ccgacgacga aatcgacctg gtcctcgact ccggcctcct cgaacacgcc gcggccatgc
     3001 tcgccgaaga agcccattcc gtgcgcctcg tcgacggcga ccatggcgcc gtgcttcttg
     3061 gcgatccgaa ccatctcggc gagcggggcg atgtcgccga gcatcgaata gacgccctcg
     3121 agcacgacca gccggccgcc ctcggccggg aggcggccca gccgcttgtc gagatcctcg
     3181 acgctgttgt ggcggaatcg gacgatctgc gcgctgccga gcgcgcagcc gtcgtagatg
     3241 gaggcgtggc tgtcggcgtc gaggatgatg aaatcctcct tgctggcgat ggtcgacatg
     3301 agcccgagat tggcctggta gccggtcgag aagaccatgg cgtggcgggt gccgtagaag
     3361 tccttgagcg cctcctcgca ttccttgtgg ccctggtagg tgccgttgag gacccggctg
     3421 ccggtggttc ccgagccata ttcgtcgagg gcgcgcttgc cggcgtcgac gacgtccggg
     3481 tcgaaggtca tgcccatgta attgtaggtg ccgagcagga tggtctcctt gccgcggacg
     3541 atcgcccggg tcggggagag cacctcctcc atgaccagcc cgaacggatc gcgcacgccg
     3601 gtgtcgagca gcgcctcgcg ctcggcgatc agggcgtcga acttggagaa gaggtcgcgg
     3661 ccctgagtgt ggccgggcgg atcgccggcc gggtcgtttg aggtggtgtc ggcctcggtc
     3721 acctaattcc tccccgagat ttctcgggga gggggaccgc gcgaagcgcg gtggaggggt
     3781 tctcccgagt cagcaaggcc cctccaccag ccttcggccg gtccccctcc cctgcaaatg
     3841 caggggagga attttccccc cgatccctca tccgtcgttc cgaagctcct cgaccgcgtc
     3901 gacgagctgg ccgaccgtct cgatctcggc ctgcatgttc atggtgatga ggatgtcgaa
     3961 ctcgtcctcg accgacgcga cgaagtcgag gacgacgagg ctgtcccact cgaggtcgcc
     4021 ggcgaaggtg gtggcgtcgg tgagctcgac gcccttcttg ttgaacggct cgatcagcgt
     4081 cgcgaccttg ccgaacgtct cttctctgtc ggtcatgccc agcccttatg tccggcggcc
     4141 cgctctgcca agcgaatagg gccgaattgg gtcaggcggg aggaagggcg ccgagcaggt
     4201 cgtgggcgaa ctggctgagg ctgtcgtcgc gggcgcccat gaccaggatc cggtcgcctg
     4261 ggcgggcgag ctcggcgagg cggcggccgg cttcggaacg gtcgccgaca tgttcggcgg
     4321 tccggccggc cgcccggacg tcgccgacga tgtcggcgcc ggtgacgacg cgctcgaccg
     4381 tgccgccgta atagaccggg tccggcatca ggagcacgtc ctcggcggcc atgttgccgg
     4441 cgaaggcggc gacgagctcg ctgcgcatcg tcctcagcgg gccgtagccg tgcggctgga
     4501 agaagatcag aagccggccc ggcgcggcgc ggcaggcggc gagggtggcg gcgatcttgt
     4561 cggggttgtg gccgaaatcg tcgatcaccg tgatcccgtt ccgggtgccg acgaggtcga
     4621 agcggcggcg caggccctcg aagccggcga gcgcttcggc ggcctcggcg aggggaaggc
     4681 cggcggcgcc cgcggcggcc agcgcggcga gcgcgttggt cacgttgtgg aggccgggaa
     4741 cgtcgagccg gacgcgggcg gtctcgccgt cctgccgct
//
LOCUS       DASVQZ010000396         7062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_852510, whole genome shotgun sequence.
ACCESSION   DASVQZ010000396 DASVQZ010000000
VERSION     DASVQZ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852510"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VF547_11165"
     CDS             complement(<1..229)
                     /locus_tag="VF547_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8263422.1"
                     /translation="MRANQTEAEERLWYYLRARRLEGEKFVRQLRIDRYLADFACRTA
                     RIAVELDGGHHSAERDAARTEIIETHGYRVLR"
     gene            436..1095
                     /locus_tag="VF547_11170"
     CDS             436..1095
                     /locus_tag="VF547_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263423.1"
                     /translation="MHRRTLLASILVIGADPWLMGEAAAQEIRIPAPTREQTAQAIAA
                     CGVPAANVRITYEVDLESDLVSISDLGGTDEARFRCLKRSVHPVYLVDVSAAPQSAAY
                     RAFADREDRRKAKAEAIAWLDARGKLGKVPRYDPAAGLDRFARDVEAACGVREGIALE
                     TFGRTRLGYRRRFFDEIMETDPHDQFLCLHEMVAASDADKHEIDFMFLGKAAPETESP
                     R"
     gene            1095..4430
                     /gene="carB"
                     /locus_tag="VF547_11175"
     CDS             1095..4430
                     /gene="carB"
                     /locus_tag="VF547_11175"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX8263424.1"
                     /translation="MPRRTDISSILIIGAGPIVIGQAAEFDYSGTQAAKTLKAEGYRI
                     ILVNSNPATIMTDPELADATYVEPITPEFVRMIIAKERPDAVLPTMGGQTALNTALAL
                     WKDGTLEEFGVKMIGADAEAIEKAEDRLKFRDAMSKIGLESPRSAIAHSLEDAMAALG
                     LVGLPAIIRPSFTLGGTGGGIAYNREEFVAIVTGGLDASPTTEVLIEESVLGWKEYEM
                     EVVRDRNDNAIIVCSIENVDPMGIHTGDSITVAPALTLTDKEYQIMRNASIACLREIG
                     VETGGSNVQFAVNSQDGRLVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IRNDITGVTPASFEPTIDYVVTKVPRFAFEKFRGAEALLSTAMKSVGEAMAIGRSFHE
                     SLQKAMRSLEIGLIGLDGVRELYGAPREEIEAALARPTPDRLLVAAQALREGLSVAEI
                     HAIAKYDPWFLERLKEIVDEEEAICRDGLPRDAAGMRRLKALGFSDRRLAELALRSAH
                     IRGGGVKTRARSHGLIHDVTKMLIGATTEDEVRALRHKLGVRPVFKRIDTCAAEFEAK
                     TPYMYSTYEAPSFGEPEDESQPSDRRKVVILGGGPNRIGQGIEFDYCCVHAAYALAEA
                     GFETIMINCNPETVSTDYDTSDRLYFEPLTVEDVLEVLHVEKSRGELVGVIVQFGGQT
                     PLKLAAALEREGIPILGTSPDAIDLAEDRERFAALVARLGLRQPEHGLARSREEAIAV
                     AERIGYPVLTRPSYVLGGRAMEIVDGPAQLDDYIVTAVQVSGDSPVLIDKYLRDAIEV
                     DVDAICDGKDVAIAGVMQHIEEAGVHSGDSACSLPPYSLEPEIVAEIERQTRSLALAL
                     GVTGLMNVQFAVKEGSVYLIEVNPRASRTVPFVAKAVGTPIAKIAARVMAGEPLMNFL
                     PLRTDVAHISVKEAVFPFARFPGIDPVLSPEMKSTGEVMGIDSDFAKAFLKSQLGAGL
                     DLPVSGTVFVSVKDSDKPAITGPVRALTELGFKALATGGTAAWLAAQGIAVEQVNKVA
                     QGRPHIVDRIKDGGVDIIFNTTEGWQSHKDSASIRATAVMGKVPYFTTAAASAAVVRA
                     IAVLQEHSLEVKSLQDYYSASRA"
     gene            4514..4993
                     /gene="greA"
                     /locus_tag="VF547_11180"
     CDS             4514..4993
                     /gene="greA"
                     /locus_tag="VF547_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8263425.1"
                     /translation="MATVDKMPMLAEGYEKLQTEVRHLKMVERPSVIEAIEVARGHGD
                     LSENAEYHAAKERQGQVEAQIADIEDRLSRAMVIDPTTLSGDKVVFGATVHLIDEDDK
                     PVTYQIVGQTEADARVGRISYNSPLGRALIGRTIGEEVEVTTPSGDRYYSIEKVEFI"
     gene            5005..5634
                     /locus_tag="VF547_11185"
     CDS             5005..5634
                     /locus_tag="VF547_11185"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8263426.1"
                     /translation="MRPPQDLRAAPVSLAIAILTAAAWLIAWIPGWQEGAVAWGGFVP
                     ADVVGGEGPVPALLTPLTSTLVHSGFVHIVFNLVMLLSCSRSVEHVLGGRGMIILYLV
                     GAYAAAAAQYAVDPEATIPMVGASGAVSAVVGAYALLFGRNRKIGRPGLATLLNALWV
                     GAAWVILQLLIGFTFETTGLRIAVAAHVGAFVAGLALARPLLLLKWRGA"
     gene            complement(5763..>7062)
                     /locus_tag="VF547_11190"
     CDS             complement(5763..>7062)
                     /locus_tag="VF547_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8263427.1"
                     /translation="GAGLAASRRRAVGFVDSDTGLANLAALERDCSGSAAVTIAFARI
                     DDFAGLLSALGAAGAAQLVRGVADRLVLGASAARIYRIEESGLAWIEPADAELADRFE
                     GVAALMRAPFAAGRKVEVRTHFGAARGDGASARQLCADAGLAALHAAEAGRTWQIFSA
                     RDSELASWRVSLLAELDSALARGELWNAYQPKLDLRTGRIGAVEALVRWDHPERGTLA
                     PDSFIPLIEQHGRAADLTLEVMARALREATEWQGAGCPLGVAVNISATLLHDQAFVGR
                     LERLLQTSGFPADRVTLEVTESAAMADPDSAIVALDRWRQLGVGVSIDDYGTGQSSLS
                     YLQRMPATELKIDRSFIAAMTAEPRNRILVRSTIAMAHELGLEVVAEGVEDQACLDLL
                     SDLGCDQVQGYLVSRPLRPEAVAAFASAARYPSPRARRTA"
BASE COUNT         1131 a         2426 c         2459 g         1046 t
ORIGIN      
        1 agcgcagcac ccggtagccg tgggtctcga tgatctcggt cctcgccgcg tcgcgctcgg
       61 cgctgtggtg acctccgtcg agctcgaccg cgatgcgcgc cgtccggcag gcgaagtcgg
      121 cgaggtagcg gtcgatccgg agctggcgga cgaacttctc gccctcgagg cggcgggctc
      181 gaaggtagta ccagagacgc tcctcggcct cggtctggtt cgcgcgcaac ttccgcgcga
      241 tcggcgtcag tcgcttcctt cccaccgccg gaccccctca cccaaccctc tccccgcggg
      301 ggagagggca gcagaaacgc accggcccgc tactccctct ccccgccggg gagagggctg
      361 gggtgagggg gctcggcgcc tttcggattt acgccgtatt tcgagcaatc ctcagtcgtg
      421 agtcaactga aaccgatgca ccgccgaacc ttattggcct ccatcctcgt catcggcgcc
      481 gacccatggc tcatgggtga ggcagcggca caggaaatca ggattcccgc gccgacccgc
      541 gagcagaccg ctcaggcgat cgcggcctgc ggggtgcccg ccgcgaacgt gcgcatcacc
      601 tacgaggtcg accttgagtc ggatctggtc agcataagcg acctgggcgg caccgacgaa
      661 gccaggttcc gctgcctgaa acgctcggtc catccggtct acctggtcga cgtctcggcg
      721 gcgccccaga gcgcggccta ccgggccttc gcggaccggg aggaccggcg caaggcgaag
      781 gccgaggcca ttgcctggct cgacgcgcgg gggaagcttg gcaaggtgcc gcgatatgac
      841 ccggccgcgg gactggacag gttcgcgcgg gacgtggaag cggcttgcgg cgttcgcgag
      901 ggcattgcgc tggaaacctt cggccggacc cgcctcggct atcgccgacg ctttttcgac
      961 gagatcatgg aaacggatcc tcatgaccag ttcctctgcc tgcatgagat ggtcgccgcg
     1021 tccgacgccg acaagcacga aatagatttc atgttcctcg gcaaggcagc ccccgagacg
     1081 gaatccccgc gctgatgccc cgccggaccg acatctcctc catcctcatc atcggcgccg
     1141 gcccgatcgt catcggccag gcggcggagt tcgattattc ggggacccag gcggccaaga
     1201 cgctcaaggc cgaagggtat cgaatcatcc tcgtcaattc gaatccggcg acgatcatga
     1261 ccgatccgga gctggccgac gcgacctatg tcgagccgat cacgcccgag ttcgtgcgga
     1321 tgatcatcgc caaggagcgg cccgacgcgg tgcttcccac catgggcggc cagaccgcgc
     1381 tcaacacggc gctggcgctg tggaaggacg gaaccctgga ggagttcggg gtcaagatga
     1441 tcggcgccga cgcggaggcg atcgagaagg cggaggaccg actgaagttc cgggacgcca
     1501 tgtcgaagat cggcctcgaa agcccgcgct cggccatcgc ccacagcctc gaggacgcga
     1561 tggccgcgct cggcctggtc ggccttccgg cgatcatccg gccgagcttc actttgggcg
     1621 gaaccggcgg cggaatcgcc tacaatcgcg aggagttcgt cgccatcgtc accggcgggc
     1681 tcgacgcctc gccgaccacc gaggtgctga tcgaggaatc ggtgctcggc tggaaggaat
     1741 atgagatgga ggtcgtccgc gacaggaacg acaacgccat catcgtctgc tcgatcgaga
     1801 acgtcgatcc gatgggcatc cacaccggcg attcgatcac ggtggctccg gcgctgaccc
     1861 tgaccgacaa ggaatatcag atcatgcgca atgcgagcat cgcttgcctg cgcgagatcg
     1921 gcgtcgagac gggcggctcc aacgtccagt tcgcggtcaa ttcgcaggac ggccggctcg
     1981 tcgtcatcga gatgaacccg cgcgtgtcgc gctcctccgc gctcgcctcg aaggcgaccg
     2041 gctttccgat cgccaaggtc gccgccaagc tcgccgtcgg ctacacgctg gacgagatcc
     2101 gcaacgacat caccggagtg accccggcca gcttcgagcc gaccatcgac tatgtcgtca
     2161 ccaaggtccc gcgcttcgcg ttcgagaagt tcaggggcgc cgaggccttg ctttcgacgg
     2221 cgatgaagtc ggtcggcgag gcgatggcga tcggccgctc gttccacgag agcctccaga
     2281 aggcgatgcg cagcctcgag atcggcctca tcggcctgga cggcgtgcgc gagctctacg
     2341 gcgcccctcg cgaggagatc gaggcggcgc tggcgcggcc gaccccggac cggctgctgg
     2401 tcgccgccca ggcgctgcgc gaggggctga gcgtcgccga gatccacgcc atcgccaaat
     2461 acgacccctg gttcctcgag cggctgaagg agatcgtcga cgaggaggag gcgatctgcc
     2521 gcgacgggct gccccgcgac gcggcgggca tgcgccggct caaggcgctc ggcttctcgg
     2581 accgccggct cgccgagctc gcgctccgct ccgcgcacat ccgcggcggc ggggtcaaga
     2641 cccgcgcgcg aagccacggc ctgatccacg acgtcaccaa gatgctgatc ggagcgacga
     2701 ccgaggacga ggtccgggcg ctgcgccaca agctcggggt gcgccccgtc ttcaagcgga
     2761 tcgacacctg cgccgccgag ttcgaggcca agacgcccta catgtactcg acctacgagg
     2821 cgccgagctt cggcgagccc gaggacgaga gccagccgtc cgaccgccgc aaggtcgtca
     2881 tcctcggcgg cggccccaac cggatcgggc agggcatcga gttcgactat tgctgcgtcc
     2941 acgccgccta cgcgctcgcc gaggccggct tcgagacgat catgatcaac tgcaatcccg
     3001 agacggtctc gaccgactac gacacgtccg accggctcta tttcgagccg ctgacggtcg
     3061 aggacgtgct cgaggtgctc cacgtcgaga agtcgcgcgg tgagctcgtc ggagtgatcg
     3121 tccagttcgg cggccagacc ccgctcaagc tggcggcggc gctggagcgg gaggggatcc
     3181 ccatcctcgg aacctcgccc gacgcgatcg acctggccga ggaccgggag cgcttcgccg
     3241 ccctggtcgc ccggctcggg ctgaggcagc ccgagcacgg cctcgcccga agccgcgagg
     3301 aggcgatcgc cgtcgccgag cggatcggct atccggtcct gacccggcct tcctacgtgc
     3361 ttggcggccg ggcgatggag atcgtcgacg gccccgccca gctcgacgac tatatcgtca
     3421 ccgcggtcca ggtgtcgggc gacagcccgg tgctgatcga caaatatctg cgcgacgcga
     3481 tcgaggtcga cgtcgacgcg atctgcgacg gcaaggacgt cgccatcgcc ggagtgatgc
     3541 agcatatcga ggaggcgggg gtccattccg gcgacagcgc ctgctcgctc ccgccctaca
     3601 gcctcgagcc cgagatcgtg gccgagatcg agcggcagac gcgctcgctc gccctcgcgc
     3661 tcggggtcac cgggctgatg aacgtccagt tcgcggtcaa ggaaggctcc gtctacctga
     3721 tcgaggtcaa tccgcgggcg agccggacgg tgcccttcgt cgccaaggcg gtcggaacgc
     3781 cgatcgccaa gatcgcggct cgggtgatgg cgggcgagcc gctgatgaac ttcctgccgc
     3841 tgcgcaccga cgtcgcccat atctcggtca aggaagcggt gttcccgttc gcgcgcttcc
     3901 ccggcatcga cccggtcctg tcaccggaaa tgaagtccac cggtgaagtc atgggaatcg
     3961 atagcgattt tgccaaggca ttcctcaagt cccagctcgg cgccgggctc gatctgcccg
     4021 tttccggcac ggtcttcgtg tcggtcaagg acagcgacaa gccggcgatc accggtccgg
     4081 tgcgggccct gaccgagctc ggcttcaagg cgctcgccac cggcggcacc gccgcctggc
     4141 tggccgccca ggggatcgcg gtggagcagg tcaacaaggt cgcgcagggc cggccgcaca
     4201 tcgtcgaccg gatcaaggac ggcggcgtcg acatcatctt caacacgacc gaggggtggc
     4261 agagccacaa ggactcggcc tcgatccggg cgacggcggt gatgggcaag gtgccctatt
     4321 tcacgaccgc cgccgccagc gcggcggtgg tcagggcgat cgcggtgctt caggagcaca
     4381 gccttgaagt gaagtccctg caggactatt attcagcctc cagagcgtga tcctgaacaa
     4441 tcgaacacgc aagacagggc gcctttcacc aaaaggcgcc cgccagagag tttttgacga
     4501 aaggtgagtg gcgatggcga cggtcgacaa gatgccgatg ctggccgagg gctatgagaa
     4561 gctgcagacc gaggtgcgcc acctcaagat ggtcgagcgc ccgtcggtga tcgaggcgat
     4621 cgaggtcgcc cgcggccatg gcgacctcag cgagaatgcc gaatatcacg cggccaagga
     4681 gcgccagggc caggtcgagg cgcagatcgc cgacatcgag gaccgcctct cgcgggcgat
     4741 ggtgatcgat ccgaccacct tgtcgggcga caaggtcgtg ttcggcgcca cggtccacct
     4801 gatcgacgag gacgacaagc cggtcaccta ccagatcgtc ggccagaccg aggcggacgc
     4861 gcgcgtcggc cggatctcct acaactcccc gctcggccgg gccctgatcg gccgcacgat
     4921 cggcgaggag gtcgaggtca ccaccccgtc cggcgaccgc tactattcga tcgagaaggt
     4981 cgagttcatc tagggcggga ggccgtgcgc ccgccgcagg acctgcgcgc cgcgccggtc
     5041 agcctcgcca tcgcgatcct caccgccgcg gcctggctga tcgcctggat tcccggctgg
     5101 caggagggcg ccgtcgcctg gggcggcttc gtgccggccg atgtcgtcgg cggggagggg
     5161 ccggtgccgg cgcttctgac cccgctgacc tcgaccctgg tccattcggg cttcgtccac
     5221 atcgtcttca acctggtcat gctgctgtcc tgcagccgct cggtcgagca cgtgctcggc
     5281 ggccgcggca tgatcatcct ctacctggtc ggcgcctatg ccgcggccgc cgcgcaatat
     5341 gcggtggatc ccgaggcgac gataccgatg gtcggggcga gcggcgccgt ctcggccgtg
     5401 gtcggcgcct atgccctgct gttcggccgc aaccggaaga tcggccgtcc gggcctggcg
     5461 accctgctca acgcgctttg ggtcggcgcc gcgtgggtga tcctgcagct cctgatcggc
     5521 ttcaccttcg agacgaccgg cctcaggatc gccgtcgccg cccatgtcgg cgccttcgtc
     5581 gccggcctgg cgctcgcgcg gccgctgctg ctgctcaagt ggcggggggc ctgagccggc
     5641 tgcgggccgg cagctgagcg ggcgtgggcc tcccgggcgc ccgacttatc gagaagaccg
     5701 atcggaagcc tgctgaccgg aaggattcct gtttatcggc ggacctcggg ccttcccggc
     5761 cgtcaggccg tcctgcgggc ccgtggactg ggatagcggg ccgcgctggc gaacgccgcg
     5821 accgcctccg gcctgagcgg gcggctgacc agatagccct ggacctggtc gcagcccagg
     5881 tcgctgagca ggtcgaggca ggcctggtcc tcgaccccct cggcgacgac ctcgaggccg
     5941 agctcgtgcg ccatggcgat ggtcgagcgg accaggatgc ggttgcgcgg ctcagccgtc
     6001 atggcggcga tgaagctccg gtcgatcttg agctcggtcg cgggcatccg ctggagatag
     6061 gagagcgacg actggccggt gccgtaatcg tcgatcgaca cgccgacccc gagctggcgc
     6121 cagcggtcga gcgcgacgat cgccgagtcg ggatcggcca tggccgccga ttcggtcacc
     6181 tccagggtga cccggtccgc cgggaagccg ctggtctgga gcagccgctc gagccggccg
     6241 acgaaggcct ggtcgtggag cagggtggcc gagatgttga cggcgacccc gagcgggcag
     6301 ccggcgccct gccattcggt cgcctcccga agcgcccggg ccatcacctc gagggtgagg
     6361 tcggcggcgc ggccgtgctg ctcgatcagc gggatgaagc tgtcgggcgc gagcgtgccg
     6421 cgctccggat ggtcccagcg gaccagcgcc tcgaccgcgc cgatccgtcc ggtgcgcagg
     6481 tcgagcttgg gctggtaggc gttccacagc tcgccgcggg ccagagccga gtcgagctcg
     6541 gcgaggaggg agacccgcca gctcgcaagc tccgaatcgc gggctgagaa gatctgccag
     6601 gtccggcccg cctcggcggc gtggagcgcg gccaggccgg cgtcggcgca cagctgccgg
     6661 gccgaggcgc cgtcgcctcg ggcggctccg aaatgggtgc ggacctcgac cttgcggccg
     6721 gccgcgaacg gggcgcgcat cagcgccgcg acgccctcga accggtcggc cagctccgcg
     6781 tcggcgggct cgatccaggc gaggccggat tcctcgatcc gatagatcct ggccgcggac
     6841 gcgcccagca ccagccggtc ggcgactccg cgcaccaatt gggccgcgcc ggcggcgccc
     6901 agcgccgaga gcaggccggc gaaatcgtcg atccgggcga aggcgatggt gacggcggcc
     6961 gagccgctgc aatcgcgctc cagggcggcg agattggcaa ggccggtgtc ggaatcgacg
     7021 aagccgaccg ctcggcggcg ggaggcggcg aggccggcgc ct
//
LOCUS       DASVQZ010000397         7047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_562612, whole genome shotgun sequence.
ACCESSION   DASVQZ010000397 DASVQZ010000000
VERSION     DASVQZ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562612"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1252
                     /locus_tag="VF547_11195"
     CDS             212..1252
                     /locus_tag="VF547_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8263428.1"
                     /translation="MSFMRFFKFPSHDMAIDLGTANTVVYVRGRGVVLNEPSVVAIET
                     INGVKRVKAVGDDAKMMMGKTPDQIEAIRPLRDGVIADIDVAEQMIKHFIQKVHGKRR
                     FPRWPEIVICVPSGSTSVERRAIRDAASNAGASQVWLIEEPMAAAIGADMPVTEPIGS
                     MVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEAISSYVRRNHNLLIGEATAERIK
                     KQVGIAKPPADGDGLSVHIKGRDLVNGVPKEITINQRQIAEALSEPVGTIVEGVRIAL
                     ENTAPELAADIVDQGIVLTGGGALLQGLDEVLRDETGLPVTVADDPLTCVAIGTGRAL
                     EEEIFRGVLLTA"
     gene            1441..2292
                     /gene="mreC"
                     /locus_tag="VF547_11200"
     CDS             1441..2292
                     /gene="mreC"
                     /locus_tag="VF547_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX8263429.1"
                     /translation="MGLFLSYVLAVGGVLFAGLMLVIAVVDPTGFNALKGLALDVTSP
                     VSSAGRGVARFVGGIGESVGNYVQAGGQNAALKKRLQESRRKLIEAQATELENKRLKA
                     LLKLTGETKDPVAVTRIVGSSFASVRRLATLSAGASSGVRPGQPVRSADGLVGRVIEA
                     GRWASRVLLVADSASNVPVRLVRDGTAAIATGHGDGTIDLKTLEVGKNPFRRGDVLVT
                     SGVGGVYPPDIPVAVVVSVQGDRTVAKPLADPSNVEYAMVLDIYQPLADQPLSEATES
                     ALRGGGQ"
     gene            2289..2804
                     /locus_tag="VF547_11205"
     CDS             2289..2804
                     /locus_tag="VF547_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX8263430.1"
                     /translation="MSRIAGSSRDVALLGFRRQWVPILSTLAASLLALFPYVATWPIV
                     PDFAYLVLLAWRLLRPEMWQAHVALPLGLFNDLAAGLPVGQSMALWTLTFLILDIVDS
                     RVGWRDYWLDWLFAAAAILFYTAGGWYVARQMGAAVPFAVLLPQLALAIFAYPLAARL
                     VVALDRWRLAR"
     gene            2801..4741
                     /gene="mrdA"
                     /locus_tag="VF547_11210"
     CDS             2801..4741
                     /gene="mrdA"
                     /locus_tag="VF547_11210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8263431.1"
                     /translation="MKRRNRPVITESSQLFTFTRRAMVLGGFQGLLGVALAGRMAWIS
                     IAENERYKLLSESNRVQLRLIPPRRGWIVDRAGQPIAINRSDFRVDLIPDRLKDPDRI
                     IAELTRLLDLPQDEVDRIREELKRAAGYQPVPVAENLPFEKYAAVTIRQPELPGVAPL
                     RAFSRFYPDGAAVAHLVGYVGTPNKAEYEKEGKAPLLITPGFKIGKEGIEKTMEPRLR
                     GRPGAERVEVTAGGKLVRQLTTLEDRSGPPLPLTIDAGLQTFAARRLGEESGSVVVLD
                     CLTGDILALASMPAYDPNSFTDGISRNEWKMLSDDERKPLLNKALNGLYPPGSTFKPM
                     VAMALLQAGIDPKARVSCPGGFQLGNRFFRCLGRHGSMTMHTAIARSCNTYFYAMGRR
                     AGIDVISAMGKRLGFGQKFDIPVVSQNYGTMPSAEWKRARYERSAKLVERPDWTESDT
                     LNTSIGQGFVIVNPLQLAVMAGRIASGRDIQPNLIQQKRAAPAALPFPKEHFETVRAG
                     MWEVVNGAGTAGRSKLEVPGIEMGGKTGTAQVRRITGGQRGQSGAWKYRDHGLFVCFA
                     PTASPRYACSVVIEHGMGGARAAAPVAKDVLTYLFDRPRALASLAAFEEQWGGTLAER
                     MEARAQRWLAEAAAQKASGGGT"
     gene            4926..6032
                     /gene="rodA"
                     /locus_tag="VF547_11215"
     CDS             4926..6032
                     /gene="rodA"
                     /locus_tag="VF547_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX8263432.1"
                     /translation="MGLDFVPAPVASLPWRLLWIVFALAAAGVLGLYSAAGGSMMPWA
                     LPHSIRFLTFLGLAIALSRIRPQTFHDLAFFAYGGGILLLILTLGLGAIKGGARSWLE
                     LGFLRLQPSELMKPIIVLTLARFYAHVPPAEVSRLWAMWPLALILGPPILLILLQPDL
                     GTAMLIILNTIALMFLAGLPLAWFIGGAVAVGVSLPIVYSFLLPHQQERVLIFLNPET
                     DPLGAGYHLTQSKIAIGSGGVFGKGFLNGTQSHLHYLPEQHTDFIFSAIAEEWGLIGG
                     CVLILLFFLLLRWGMSVALDSKGRFERLAAAGLTLTIFFYIAVNLMMVMGLAPVVGIP
                     LPLVSYGGSAMLTVMICLGILMSIERHNRGERRP"
     gene            complement(6094..>7047)
                     /locus_tag="VF547_11220"
     CDS             complement(6094..>7047)
                     /locus_tag="VF547_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX8263433.1"
                     /translation="ALHEHPRVRALLARREELWPMKLGRILLGLAMTGLAVELALIPI
                     ALWHFHQAGVYGALANIVAIPLTTFVVMPLEALALLLDLAGLGAPCWWLAGAALDLLL
                     ALAHATAGAPGAVARLPTLPTAAFALMAGGGVWLALWRTRWRRWGLVPAAAGAAAALA
                     APAPDLLVTGDGRHLAIRTESGELALLRGRAGDYVRGLLSQSAGMEGEMGELELLPAA
                     SCSADFCAARVRGWRLLASRSARRVPLEALRRACAEADIVVSDRRLPAACRPAWLKAD
                     RDFLARSGGLAVTLGPRPRVVTVAGGDRHPWIGAAPRGSRP"
BASE COUNT         1031 a         2530 c         2439 g         1047 t
ORIGIN      
        1 tttccaccac ttcaccggcg gcgatgcggt tgaccagatg ctcgggcagg cggcgtattg
       61 acatgcccct cgtccctagc ccaagccgcc ccgtcccgcg aggcttgtta aacctttggt
      121 ggaacaagcc gaagggccat cctatagaga aaggcgagcg cgccgggcat cacgccgcgg
      181 cggcgttgtg aaagaagcgg gacccacctc gatgtcattc atgcgtttct tcaagttccc
      241 ctcccacgac atggcgatcg acctcggaac ggcgaacacc gtcgtctacg tgcgcgggcg
      301 aggcgtggtc ctgaacgagc cgtcggtggt cgcgatcgag acgatcaacg gggtgaagcg
      361 cgtgaaggcg gtcggcgacg acgccaagat gatgatgggc aagaccccgg accagatcga
      421 ggcgatacgg ccgcttcggg acggggtcat cgccgacatc gacgtcgccg agcagatgat
      481 caagcatttc atccagaagg tgcacggcaa gcgccgcttc ccgcgctggc cggagatcgt
      541 gatctgcgtg ccgtcgggct cgaccagcgt cgagcggcgc gcgatccgcg acgcggcgtc
      601 caacgcgggc gcgtcacagg tgtggctgat cgaggagccg atggcggccg cgatcggcgc
      661 cgacatgccg gtgaccgagc cgatcgggtc gatggtcgtc gacatcggcg gcgggacgac
      721 cgaggtggcg gtgctgtcgc tccgcgggct cgcctacacg acctcggtgc gggtcggcgg
      781 cgacaagatg gacgaggcga tctcctccta cgtgcggcgc aaccacaatc tgctgatcgg
      841 cgaggcgacg gccgagcgga tcaagaagca ggtcgggatc gcgaagccgc cggccgacgg
      901 cgacggcctt tccgtccaca tcaagggccg cgacctcgtc aacggggtgc ccaaggagat
      961 cacgatcaac cagaggcaga tcgccgaggc gctttcggag ccggtcggga cgatcgtcga
     1021 gggggtgagg atcgcgctcg agaacacggc gcccgagctc gccgccgaca tcgtcgacca
     1081 gggcatcgtc ctgaccggcg gcggagccct gctgcagggc ctggacgagg tgcttcgcga
     1141 cgagaccggg ctgccggtga cggtcgccga cgaccccttg acctgcgtcg ccatcggcac
     1201 cggacgggcg ctcgaggagg agatcttccg cggcgtcctc ctgaccgcct gaggcccggc
     1261 cccggggagg tcgcccgatg tccacgcctt ccccgtcctg tcggcgaaag ccggcatcca
     1321 ttcggtctct gccgagcttc gccggagacg gcgcacggac cccagcctcc gctggggtga
     1381 cggcttactg acgtgccgcc gccgtcgacc cgacgcgccg gcttctcgcg ccgggcccag
     1441 atggggctgt tcctgtccta cgtgctggcg gtcggcggag tgctgttcgc cgggctgatg
     1501 ctggtcatcg ccgtcgtcga cccgaccggc ttcaacgcgc tcaagggcct cgccctcgac
     1561 gtcaccagcc cggtgagctc ggccgggcgc ggagtggcgc gcttcgtcgg cggaatcggc
     1621 gaatcggtcg gcaattacgt ccaggcgggc gggcagaatg cggctctgaa gaagcggctt
     1681 caggaatcgc ggcgcaagct gatcgaggcg caggcgaccg agctcgagaa caagaggctg
     1741 aaggcgctgc tgaagctgac cggcgagacc aaggacccgg tcgcggtgac ccggatcgtc
     1801 ggctcgtcct tcgcctcggt ccgccggctc gccacgctgt cggccggagc ctcgtccggg
     1861 gtccggccgg gccagccggt gcgctcggcc gacgggctgg tcgggcgggt gatcgaggcc
     1921 gggcgctggg cgagccgggt cctgctcgtc gccgacagcg ccagcaacgt gccggtgcgg
     1981 ctggtccgcg acggcaccgc ggcgatcgcc accggccatg gcgacggcac catcgacctc
     2041 aagaccctgg aggtgggcaa gaatccgttc cgccgcggcg acgtgctggt gacgtccggc
     2101 gtcggcgggg tctatccgcc cgacatcccg gtcgcggtgg tggtgtcggt ccagggggac
     2161 cggacggtgg ccaagccgct cgccgacccc tccaacgtcg aatatgcgat ggtgctcgat
     2221 atctaccagc cgctcgccga ccagccgctc tccgaggcga ccgagagcgc gctcaggggg
     2281 ggcgggcagt gagccggatc gccggatcga gccgcgacgt cgccctgctc ggcttccgcc
     2341 gccagtgggt gccgatcctc agcacgctcg cagcctcgct gctcgccctc ttcccctatg
     2401 tcgcgacctg gccgatcgtt ccggatttcg cctatctcgt cctgctcgcc tggcggctcc
     2461 tgaggccgga aatgtggcag gcccacgtcg ccctcccgct cggcctgttc aacgatctcg
     2521 ccgccggcct gccggtcggc cagtccatgg ccctgtggac cctgaccttc ctcatcctcg
     2581 acatcgtcga cagccgggtc ggctggcgcg actactggct cgactggctg ttcgcggccg
     2641 ccgcgatcct gttctacacc gccggcggct ggtacgtggc gcggcagatg ggcgcggcgg
     2701 tgccgttcgc cgtcctcctc ccgcagctcg ctctggcgat cttcgcctat ccgctggcgg
     2761 cgcggctggt cgtcgccctc gaccgctggc ggctggcgcg atgaagcggc gcaaccgccc
     2821 cgtcataacc gagagcagcc agctcttcac cttcacccgc cgcgcgatgg tcctgggcgg
     2881 cttccagggc ctcctcgggg tcgcgctcgc cggccgcatg gcctggatct cgatcgccga
     2941 gaacgagcgc tacaaactgc tctccgagag caaccgggtc cagcttcgcc tgatcccgcc
     3001 gcggcggggc tggatcgtcg accgcgccgg ccagccgatc gcgatcaacc ggagcgactt
     3061 ccgggtcgac ctcatcccgg atcggctcaa ggacccggac cggatcatcg ccgagctgac
     3121 ccggctgctc gaccttcccc aggacgaggt cgaccggatc cgggaggagc tcaagcgcgc
     3181 cgccggctac cagccggtgc cggtcgccga gaacctgccg ttcgagaaat atgcggcggt
     3241 gaccatccgc cagcccgagc tgcccggggt cgccccgctg cgggccttct cgcgcttcta
     3301 cccggacggc gccgccgtcg cgcatctggt cggctatgtc ggcactccca acaaggcgga
     3361 atatgagaag gagggcaagg cgccgctcct gatcacgccg ggcttcaaga tcggcaagga
     3421 aggcatcgag aagacgatgg agccgcggct gcggggacgg ccgggcgcgg agcgggtcga
     3481 agtcaccgcg ggcggcaagc tggtgcggca gctgaccacc ctcgaggacc gcagcggccc
     3541 gccgctgccg ctcaccatcg acgccgggct ccagaccttc gcggcgcggc gcctcggcga
     3601 ggaatcgggc tcggtggtgg tgctcgactg cctcaccggg gacatcctcg ccctggcctc
     3661 gatgccggcc tacgatccca acagcttcac cgacggcatc agccgcaacg aatggaagat
     3721 gctgtcggac gacgagcgca agccgctgct caacaaggcg ctgaacggcc tctacccgcc
     3781 cggctcgacc ttcaagccga tggtggcgat ggccttgctc caggcgggga tcgaccccaa
     3841 ggcccgggtc agctgtcccg gcggcttcca gctcggcaac cgcttcttcc gctgcctcgg
     3901 ccgccacggc tcgatgacca tgcacaccgc catcgcgagg agctgcaaca cctatttcta
     3961 cgccatgggc aggcgcgccg ggatcgatgt catttcggcc atgggcaagc gcctcggctt
     4021 cggccagaag ttcgacatcc cggtcgtctc ccagaattac ggcacgatgc cgagcgcgga
     4081 atggaagcgg gcgcgctacg agcgcagcgc caagctggtc gagcggccgg actggaccga
     4141 atcggatacg ctcaacacgt cgatcggcca gggcttcgtc atcgtcaatc cgctgcagct
     4201 ggcggtgatg gccggccgga tcgcgtccgg ccgcgacatc cagcccaacc tgatccagca
     4261 aaagcgggca gcgccggccg cccttccctt cccgaaggag catttcgaaa ccgttcgcgc
     4321 cgggatgtgg gaggtcgtga acggggccgg cacggccggg cgaagcaagc tcgaggtccc
     4381 cgggatcgag atgggcggca agaccggaac cgcccaggtc cgccggatca ccggcggcca
     4441 gcgcggccag tcgggcgcct ggaaatatcg cgaccacggc ctgttcgtct gcttcgcccc
     4501 gaccgccagc ccccgctacg cctgctcggt ggtgatcgag cacggaatgg gcggtgcccg
     4561 ggccgccgct ccggtcgcca aggacgtgct cacctatctg ttcgacaggc cgagggcgct
     4621 cgcctcgctc gccgcgttcg aggagcaatg gggcggcacc ctcgccgagc ggatggaggc
     4681 tcgggcccag cgctggctgg ccgaggcggc cgcgcagaag gcctccggcg gcgggacgtg
     4741 agggcgcttg tcatcctcaa ttcctccccg agacttctcg gggagcggga ccgcgcgaag
     4801 cgcggtggag gggttcttcg gactcggcaa ggccccgcca ccgcccttcg gacggtcccc
     4861 ctcccctgca aaagcagggg aggaactaag gccggcccgc gctcctccga tcgggaccgg
     4921 ttttaatggg cctcgatttc gtcccggccc cggtcgcgag cctgccgtgg cggctgctct
     4981 ggatcgtctt cgccctggcg gcggccggag tgctcggcct ctattcggcc gccgggggat
     5041 cgatgatgcc ctgggcgctg ccccattcga tccgcttcct gacgttcctc ggactcgcca
     5101 tcgccttgtc gcggatccgc ccgcagacct tccacgacct cgccttcttc gcctacggcg
     5161 gcgggattct gctgctcatc ctgaccctcg gtctcggggc gatcaaaggc ggcgcgagga
     5221 gctggctcga gctcggcttc cttcgcctcc agccgtccga gctgatgaag ccgatcatcg
     5281 tcctcaccct cgcccgcttc tacgcccacg tcccgcccgc ggaggtcagc cggctgtggg
     5341 cgatgtggcc gctcgccctc atcctcgggc cgccgatcct gctcatcctg ctccagcccg
     5401 acctcgggac cgcgatgctg atcatcctca acacgatcgc cctgatgttc ctcgccggac
     5461 tgccgctggc ttggttcatc ggcggagccg tcgcggtcgg ggtctcgctt ccgatcgtct
     5521 attccttcct tctgccgcac cagcaggagc gggtcctcat cttcctcaac ccggagaccg
     5581 acccgctcgg cgccggctat catctcaccc agtcgaagat cgcgatcgga tcgggcgggg
     5641 tgttcggcaa gggcttcctc aacggcaccc agagccacct ccattacctg cccgagcagc
     5701 atacggactt catcttctcg gccatcgccg aggaatgggg gctgatcggg ggctgcgtgc
     5761 tcatcctcct cttcttcctc ctcctgcgct gggggatgag cgtggcgctg gactcgaagg
     5821 ggcggttcga gcggctggcg gcggcgggcc tcactctgac catcttcttc tacatcgccg
     5881 tcaacctgat gatggtgatg ggcctcgccc cggtcgtcgg aatccccctc cccctcgtct
     5941 cctacggcgg gtcggcgatg ctgacggtga tgatctgcct cggcatcctg atgtcgatcg
     6001 agcgccacaa ccgcggcgag cggcggccct gatccccgcc ccgtcgcgga tggcggaaac
     6061 tcgctggggc ggatcgagcc gggggacgga ttttcagggt cgcgagccgc gaggggcggc
     6121 cccgatccag ggatgccgat cgccacccgc caccgtcacg acgcgcggcc gcggtccgag
     6181 cgtcaccgca agcccgccgc tccgcgccag gaagtcgcgg tccgccttga gccaggccgg
     6241 ccggcaggcg gcgggcagcc gccggtccga gacgacgatg tccgcctccg cgcaggcgcg
     6301 ccgcagcgcc tcgagcggca cccgccgggc gcttcggctg gcgagcagcc gccagccgcg
     6361 cacccgggcg gcgcagaaat cggcgctgca cgaggccgcg ggcagaagct ccagctcgcc
     6421 catctcccct tccatccccg cgctctggga cagcaggccg cggacatagt cgccggcgcg
     6481 gccgcgcagc agcgcgagct caccggattc ggtccggatc gcgaggtgcc ggccgtcgcc
     6541 ggtgacgagc aggtccggcg ccggggccgc cagcgccgcc gcggcgcccg ccgcggccgg
     6601 gacgagcccc cagcgccgcc agcgcgtgcg ccacaaggcg agccagacgc cgccgccggc
     6661 catcagcgcg aaagccgccg tcggcagggt cggcagcctc gcgaccgccc ccggcgcgcc
     6721 cgcggtggcg tgggcgagcg cgaggaggag gtcgagcgcg gctccggcca gccaccagca
     6781 aggcgcgccg agcccggcca ggtcgagcag cagggccagc gcctcgagcg gcatgacgac
     6841 gaaggtggtg agcgggatgg cgacgatatt ggcgagcgcc ccgtacaccc cggcctggtg
     6901 gaaatgccac agggcgatcg ggatcagggc gagctccacg gccagcccgg tcatggcgag
     6961 gccgaggagg atccggccga gcttcatcgg ccagagctcc tcgcgccggg cgagcagggc
     7021 ccggacgcgc ggatgctcgt gcagggc
//
LOCUS       DASVQZ010000398         9133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_948187, whole genome shotgun sequence.
ACCESSION   DASVQZ010000398 DASVQZ010000000
VERSION     DASVQZ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948187"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..604
                     /locus_tag="VF547_11225"
     CDS             194..604
                     /locus_tag="VF547_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8263434.1"
                     /translation="MGPYALDFFCHQSGLAIEVDGIAHEMGDNPRRDARRDAWLAERG
                     VMTFRVTAADVLGETEAVVRMVVHLCRERSPPPNSSPRFRGEGDRLKAGGGVLPDSAR
                     SPSTRLRLVPLPCKGKGGILFDQRRGQILPWRRR"
     gene            complement(565..2502)
                     /locus_tag="VF547_11230"
     CDS             complement(565..2502)
                     /locus_tag="VF547_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8263435.1"
                     /translation="MLRFRAGAPTQFSIAVQLAAAAWLACALLQLLLYLRASPYGGPF
                     LLEWRRYFPYALYYELMGVWLVALPFLIHWLVRPAGGRANAVHGLQAVLLALYVALSQ
                     LDHEVLRFLGIRLGTAFIATYARPGTVADSLFFDVLRDDTGGAYLPLLLLVGAPLLYL
                     GWAARRILRAAPVAVRPRLWPAALAVLLPIAAPLNGWTKATGQFRLRKVEPVTFAIAV
                     DAALGLEDRRRPADYDRRVRDQQGRWLAESADKGWRFPDPNYPHLRVPDRPSEPAASE
                     RWNVIFVQLETFRGAEMGFLGRRPAPTPFLDRLAAGPAAAVWTRALSFGPPSINGLFA
                     GHCSAAPHSRRYMTSFTHAGFYCLPEALAKRGYRTEMFNAGDTDWDNGTWWIGRWYHR
                     LWRYPEAKEQDRPVFRAAARRIRALGAAGPFLATLVSVSNHTPFRSREPALDMAGHGS
                     PRERILNTTRYTDDVLREFVESLRGEPWFERTLLVIVGDHGFNMGEHDRRPGQQNLYR
                     ESTWVPLIVAGAHPRLPAGRHSRLASQLDIAPTLADLLGLREPNPWQGHSLLGAGGPR
                     VLHARNRAMLAADTPAWSLVTDPLDGRARLFDSRTDWAGQDRDLAAAHPQIAAALAAR
                     AREAAELNDHLLRQGRIWPRR"
     gene            2614..3177
                     /locus_tag="VF547_11235"
     CDS             2614..3177
                     /locus_tag="VF547_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263436.1"
                     /translation="MNRQKPAAYRPEFEQALRLFGRASEAMKARGLPAPVLVGGAAVE
                     IYSKGRINTGDFDIATPWQAELEDELEGLGFIRPTGPGRATRGLIHPEFRLGFEIVSS
                     SLLDGMADCERVQLIDLGEDGEAAVISVEDIIADRMGQFASGTAPEMLVQAKRLFSLH
                     PDADRHYMEKRIRHETAGDYGIEDLKS"
     gene            3188..3349
                     /locus_tag="VF547_11240"
     CDS             3188..3349
                     /locus_tag="VF547_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263437.1"
                     /translation="MPLADFAADIDRRRAEYEERLGRPFDIPRNSGGRRTASKRALLK
                     AIADSGGRW"
     gene            3682..5343
                     /locus_tag="VF547_11245"
     CDS             3682..5343
                     /locus_tag="VF547_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8263438.1"
                     /translation="MRLSALLALSLLAACASAPRQPEAARIDPVRMSNMVRTLASDEY
                     EGRAPGTAGEAKTIAYISEQFRLAGLEPAGENGGWTQKVPLVRTQLGKGGSVSVAARG
                     GRTALRVPDDVYLSTVRETGRARIDGAPMVFVGYGIAAPERPWDDFKGVDLAGKVAVF
                     LVNDPDFEAPADDPVAGRFGGQAMTYYGRWTYKFEEAARRGAIAALVVHETAGAGYGW
                     NTVQAPGGENYNIVLKPGAPQPVLLQGWIQRSVTEDLFRRAGLDFEAVKRAARTPAFR
                     PIDLDARLSADFRVEAIRVESHNVLGKIAGTERPDETIWFASHWDAYGVGAPDARGRT
                     VRPGALDSASGIAGIVELARAFKAGPRPRRTLVFAAWTAEERGLLGAEYYATSPLFPM
                     DRAVANLTLDTLQPNGLARDVVLIGKGQSDLGERLAEAAARQRRTVTPDARPERGLFY
                     RADHFAFARRGVPVLLLMALGGGVDLVEGGREAGDRWVTEFTAQCYHQTCDEWRPELD
                     FSGAAQDVALFHDIGRALAFGRDWPQWNSGSEFRAIRAQSAAARR"
     gene            complement(5373..6344)
                     /locus_tag="VF547_11250"
     CDS             complement(5373..6344)
                     /locus_tag="VF547_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8263439.1"
                     /translation="MAKALRRRGRELAGGATLLATASCLPLQAAPSPDMTAAALVAAE
                     STAPSPEPMVLRQVAPTEALKINAAIPFSAEPNPRAPSTVFRAAGVGDQLRSLQCLAE
                     AIYYEARSESEEGQRAVAQVVLNRVRHPAYPGSVCGVVYQGPLRAGGGCQFTFTCDGS
                     LASAPSGLGWLRARRLAAEALAGYVYAPVGLATNYHTHQVLPDWAFRLAKATVIGNHI
                     FYRMPGAWGGPGAFSQIYRGREPSPATVMASRLPINLGRPAAATLAAPVDLSVLALPR
                     LPASSTVVTEAPPPPVNDRLPRSTVKPEFENSGRWLDEVPAAATPAR"
     gene            6585..8162
                     /locus_tag="VF547_11255"
     CDS             6585..8162
                     /locus_tag="VF547_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX8263440.1"
                     /translation="MRKILACVSLAALLAGGCDFVSGGGGGSSGQAVQVDPKAVTAAD
                     LQAAVSDPRVKRFYEARGWQAVWTGERAEELNAALGDAVRHAIDAKSFVEHATKGASA
                     AEREAGLTLAAIEYGQALATGAVDPRKIYEVYTVPMPKADVVGGLGRAVDQGGVRQWL
                     AGLAPQDTEYKALSDAYVAYRQKAAQEQRAAVAAGEQIKPGKSDPRIPQIRQGLQANG
                     YLQAAASAPSGDSKAKAKATAVRADGTVYSADMVAAVKKVQTDYGIEPDGVIGNSTLE
                     ALNTGARDRARILAVNLERRRWLERTPPATRIDVNTAAAELHYWKDGSEKDRRRVVVG
                     QPDWETPELGSPIFRLVANPPWTVPESIAEEEILPKGAAYMAKENIVMKNGRLVQEPG
                     PKSALGLVKFDMQNDEQIYLHDTPAKALFASTQRHASHGCSRVENAVEFARLLAQDDG
                     KLEEFDKALATKKETAVELSSKIPVRLMYHSAYLAGGRVVFRTDPYGWDDKLAAALGL
                     GGQVRPRVKTHVADVGP"
     gene            complement(8309..8473)
                     /locus_tag="VF547_11260"
     CDS             complement(8309..8473)
                     /locus_tag="VF547_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263441.1"
                     /translation="MRGNSGKGEGTNNNRNPTLKDHERDRGRSGEAIAGEKKAATGAD
                     RIDTKDKQSS"
     gene            8727..9062
                     /locus_tag="VF547_11265"
     CDS             8727..9062
                     /locus_tag="VF547_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEX8263442.1"
                     /translation="MFHRTLSALAAAALTVGAFAFTAPAQAAAADDSVTIALDGLNPA
                     DPADADRIARRIRNAARGLCGSQLIQPTRLMQHAAACEKAVVAEARTAVETAAARQGG
                     PFRLTLRSN"
BASE COUNT         1391 a         3081 c         3329 g         1332 t
ORIGIN      
        1 cgaatcagct cggctcgact atgcggcgtc aaacgggcat tcttgtggat gttcatccga
       61 ctctcctgag gatctgaagc ttcgcaacct cagtctcccc ggttcgggtc ggatggacaa
      121 cctactgaaa gctcacacct agcgcgagct tcgagcccgg cccggcgggt tcaagttccg
      181 ccgccagcat ccgatcggcc cctacgcgct cgacttcttc tgccatcaat cgggcctcgc
      241 catcgaggtg gacggcatcg cccacgagat gggcgacaat ccgcggcggg atgcgcggcg
      301 cgacgcctgg ctggcggagc ggggggtgat gacgtttcgc gtgacggccg cggacgtgct
      361 cggagagacg gaggccgtcg ttcggatggt cgtgcatctt tgccgggagc gatctccgcc
      421 ccctaattcc tccccgcgat tccgcgggga gggggaccgg ctgaaagccg gtggaggggt
      481 tcttccggac tcggcaaggt ccccctccac caggcttcgc ctggtccccc tcccctgcaa
      541 aggcaaagga ggaattttgt tcgatcagcg ccgcggccag atccttcctt ggcgcaggag
      601 gtgatcgttg agctcggcgg cctcgcgggc gcgggcggca agggcggcgg cgatttgggg
      661 gtgggcggcg gcgaggtcgc ggtcctggcc ggcccagtcg gtgcgggagt cgaacagccg
      721 cgcccggccg tccagcggat cggtgaccag cgaccaggcc ggcgtgtcgg ccgcgagcat
      781 cgcccggttg cgggcatgga gcacgcgggg tccgccggcg cccaggaggc tgtgcccctg
      841 ccaggggttg ggctcgcgca gcccgagcag gtcggccagg gtcggggcga tgtcgagctg
      901 gctcgccagc cgggaatggc ggcccgcggg aagccgcgga tgggcgccgg cgacgatcag
      961 cggcacccag gtcgattccc ggtagaggtt ctgctggccc gggcggcggt cgtgctcgcc
     1021 catgttgaag ccgtggtcgc cgacgatcac cagcagcgtg cgctcgaacc agggctcgcc
     1081 ccggagcgac tcgacgaact cgcgcagcac gtcgtcggtg tagcgggtgg tgttgaggat
     1141 ccgctcccgc ggcgagccgt ggccggccat gtccagggcg ggctcgcggg agcggaaggg
     1201 cgtgtggttg ctgaccgaga cgagggtcgc gaggaacggg cccgccgccc cgagcgctcg
     1261 gatccggcgg gcggcggcgc ggaagaccgg gcggtcctgc tccttcgcct cgggatagcg
     1321 ccagagacgg tggtaccagc ggccgatcca ccaggtgccg ttgtcccagt cggtgtcgcc
     1381 ggcgttgaac atctcggtcc ggtagccgcg cttcgcgagc gcctcgggca ggcagtagaa
     1441 gccggcatgg gtgaacgagg tcatgtagcg gcgcgaatgg ggcgcggccg agcaatggcc
     1501 ggcgaacagg ccgttgatgc tcggcgggcc gaagctcagc gcccgggtcc agacggcggc
     1561 ggccggcccg gcggcgaggc ggtcgaggaa gggggtgggg gcggggcgcc ggccgaggaa
     1621 gcccatctcg gcgccgcgga acgtctcgag ctggacgaag atgacgttcc agcgctcgga
     1681 cgcggccggc tccgacggcc ggtccgggac gcggagatgg ggatagttgg gatcggggaa
     1741 gcgccacccc ttgtccgcgc tctcggccag ccagcgcccc tgctggtcgc gaacccggcg
     1801 gtcgtaatcg gccggccggc ggcggtcctc gaggccgagg gccgcgtcga cggcgatggc
     1861 gaaggtgacc ggctccacct tgcgcagccg gaactgaccg gtcgccttgg tccagccgtt
     1921 gagcggcgcc gcgatcggca ggaggacggc cagagcggcg ggccagagcc gcggccggac
     1981 tgcgaccggc gcggcgcgga ggatccgccg ggccgcccag ccgagataga ggagcggcgc
     2041 cccgacgagc aggagaaggg gcagataggc cccgccggta tcgtcgcgaa gcacgtcgaa
     2101 gaacaggctg tcggctaccg tcccgggccg ggcataggtg gcgatgaagg cggtcccgag
     2161 acggatgccg aggaatcgga gcacttcatg gtcgagctgg ctgagcgcga cgtagagcgc
     2221 gagcaggacg gcctgaagcc cgtgcaccgc gttcgcccgg ccgcccgccg gccggaccag
     2281 ccagtggatc aggaagggca gggcgacgag ccacacgccc atcagctcgt agtagagggc
     2341 gtaggggaaa tatctgcgcc actcgagcag gaacggcccg ccataggggc tggcgcgcag
     2401 gtagagcagc agctggagca gggcgcaggc gagccaggcc gccgcggcga gctggaccgc
     2461 gatcgagaat tgggtcggag cgccggcccg aaacctcagc atgcggccaa tcccccaagc
     2521 caaccgccct aagcgggacg ttagcctctc acctcccggc gggccagcgc cggacttgcg
     2581 cggtatatct tcatcacgca ctacttcgca gcgatgaaca ggcagaaacc cgccgcctac
     2641 cgcccagagt tcgagcaggc gctgcggctc ttcggccgag ctagcgaggc catgaaggcg
     2701 cgcgggcttc cagcccccgt tctggttggc ggcgcggccg tcgaaatcta ctcaaaggga
     2761 aggatcaaca ccggagattt cgatatagcc acgccttggc aggcggagct tgaggatgag
     2821 ctcgaaggtc tcggcttcat acgtccaacg ggacccggtc gcgccactcg cggtctgatc
     2881 cacccggagt tccggcttgg cttcgaaatc gtctcttctt cactgctcga cggcatggcc
     2941 gattgcgagc gggtccagtt gatagacctt ggcgaggatg gcgaggccgc cgtcatctcg
     3001 gtcgaagata ttatcgccga tcgcatgggg caatttgcgt cgggcactgc gcccgagatg
     3061 ctcgtccagg ccaagcgatt gttctcgctt catcccgatg ccgaccggca ctatatggag
     3121 aaacgcatcc ggcacgaaac cgcaggcgat tatggaatcg aagacctcaa gagctgagcg
     3181 gaaatcggtg ccgctggcgg acttcgccgc ggacatcgat cgccggcgcg ccgagtatga
     3241 ggaacgcctc ggccgcccgt tcgacatccc ccgcaacagc ggcggtcgcc ggacggcgag
     3301 caagcgcgcg cttctcaagg cgatcgcgga ttcgggtggc cgttggtgac ccggcgccag
     3361 gctccttgac ccgacatggt gttcatgcta tgttcgctca tttgagaatg ggcgatccgt
     3421 gtctgtttgg cgatcccgtc cggcaagacg aagcctcccg actggatggg tgactccccg
     3481 ttcggatcgc cgtcgcgaaa cggacgcttc tactgaaaac aaaggattct tcggcccgac
     3541 atgcgggtct tttgtgtctt ttggagcccc gcaaaaaaag cggtcccgtg tccaattcgt
     3601 cccggtcatc cacttcggcc gatcaccggg caaccgaaaa ggcggcggac ggtttgggaa
     3661 ggcagccccg ggagccgccc tatgagactg tccgccctgc tcgccttgtc gctcctcgcc
     3721 gcctgcgcgt cggccccccg gcagcccgaa gccgcgcgga tcgaccccgt ccggatgtcg
     3781 aacatggtcc ggacgctcgc ctcggacgaa tatgagggcc gcgcgcccgg aacggccggc
     3841 gaggcgaaga cgatcgccta tatttccgag cagttccggc tcgccgggct cgagcctgcc
     3901 ggcgagaacg gcggctggac ccagaaggtg ccgctggtgc ggacgcagct cggcaagggg
     3961 ggctcggtct cggtcgcggc gaggggcggg cggacggcgc tccgggtccc cgacgacgtc
     4021 tatctttcga ccgtccgcga gaccgggcgg gcgcggatcg acggcgcgcc gatggtgttc
     4081 gtcggctatg gaatcgccgc gcccgagcgg ccgtgggacg atttcaaggg cgtcgacctc
     4141 gccggcaagg tcgccgtctt cctcgtcaac gatcccgatt tcgaggctcc ggcggacgac
     4201 ccggtcgccg gcaggttcgg cggccaggcg atgacctatt acggccgctg gacctacaag
     4261 ttcgaggagg cggcccggcg cggggcgatc gccgccctgg tcgtccacga gaccgccggc
     4321 gcgggctacg gctggaacac ggtgcaggcg cccggcggcg agaattacaa catcgtcctg
     4381 aagcccggag cgccgcagcc ggtcctcctg cagggctgga tccagcgcag cgtcaccgag
     4441 gacctgttcc ggcgcgccgg cctcgacttc gaggcggtga agagggcggc gcgcaccccg
     4501 gccttccgcc cgatcgacct cgacgcccgc ctctcggccg atttccgggt cgaggcgatc
     4561 cgggtcgaga gccacaacgt gctcggcaag atcgccggga cggagcggcc ggacgagacc
     4621 atctggttcg ccagccactg ggacgcctat ggcgtcggag cgcccgacgc ccggggcagg
     4681 acggtccggc ccggcgcgct cgacagcgcc tccggcatcg ccgggatcgt cgagctcgcc
     4741 cgcgccttca aggccgggcc gaggccgcgg cggactctgg ttttcgcagc gtggaccgcg
     4801 gaggagaggg gcctgctcgg cgccgaatat tatgcgacga gcccgctctt cccgatggac
     4861 cgggcggtcg ccaacctcac cctcgacacg cttcagccca acggcctggc gcgcgacgtc
     4921 gtgctgatcg gcaagggcca gagcgatctc ggggagcggc tcgccgaggc ggcggcgcgg
     4981 cagcgccgga ccgtgacccc cgacgcccgg cccgagcgcg ggctcttcta ccgggccgac
     5041 catttcgcct tcgcccggcg cggcgtgccg gtgctcctgc tgatggccct gggcggcggg
     5101 gtcgacctgg tcgagggcgg gcgcgaggcg ggcgaccgct gggtcaccga gttcaccgcc
     5161 caatgctacc atcagacctg cgacgaatgg cggccggagc tcgacttcag cggcgccgcg
     5221 caggacgtcg ccctgttcca cgacatcggc cgcgcgctcg ccttcggccg cgactggccg
     5281 caatggaatt cgggctcgga gttccgggcg atccgggcgc aaagcgctgc ggcccggcgt
     5341 tagcgctccc cggccttcgc cggggaacgg gatcagcgag ccggagtcgc ggccgccggc
     5401 acctcgtcga gccagcggcc gctgttctcg aactcgggct tgaccgtcga gcgcggcagg
     5461 cggtcgttga ccggaggcgg cggcgcttcc gtcaccacgg tcgaggaggc gggaagacgc
     5521 ggcagggcga ggacgctgag gtcgaccggg gcggcgagcg tggccgccgc cggccggccg
     5581 aggttgatcg ggagccggct cgccatcacc gtcgccggcg acggctcgcg gccgcgatag
     5641 atttgcgaga aggcgccggg gccgccccag gcgcccggca tgcggtagaa gatgtgattg
     5701 ccgatcaccg tcgccttggc gagtcggaac gcccagtcgg ggagcacctg gtgggtatgg
     5761 tagttggtcg ccaggccgac cggcgcgtag acgtagccgg ccagcgcctc ggcggcgagg
     5821 cggcgggcgc gcagccagcc caggcccgac ggcgccgagg cgagcgagcc gtcgcaggtg
     5881 aaggtgaact ggcagccccc gccggcgcgc agcgggccct ggtagacgac tccgcagacg
     5941 ctgcccggat aggccggatg gcggacccgg ttgaggacca cctgggcgac ggcgcgctgg
     6001 ccctcctcgc tctcggagcg ggcctcgtaa taaatggctt cggcgaggca ctgcagcgag
     6061 cgcagctggt cgccgacgcc ggcggcgcgg aagacggtcg agggcgcgcg cgggttgggc
     6121 tcggcggaga aggggatggc ggcgttgatc ttcagggctt cggtgggcgc gacctggcgc
     6181 agcaccatcg gctcggggga gggggcggtc gactccgccg ccacgagcgc cgcggccgtc
     6241 atgtcgggcg acggcgcggc ctgaaggggc aggcaggaag cggtggcgag cagcgtcgcg
     6301 ccgccggcca gctcgcgccc gcgccggcga agcgcttttg ccaagacctt gaaattaggg
     6361 gaaatccgta cggtcaacct ggttctcgcc gcctgttcga atgccccgcc tagcttccga
     6421 tagctaatat tgttgcacca gcgcgccccc ccattttccg ctgtcccgtt ccgggacggc
     6481 cgagcgtcgg cggttaagcc gaagaggcgc ttgtttttgt tcggatcgta ttggcgcgcg
     6541 gggacgtttt ggggggatcc aaccctctcc tagcggcggt gtgtatgcgt aagattctgg
     6601 cgtgcgtttc gctggcggcc cttctggccg gcggctgcga tttcgtgagc ggcggcggcg
     6661 gcggaagctc gggccaggcc gtccaggtcg atcccaaggc ggtcacggcc gcggacctcc
     6721 aggcggcggt gagcgatccg agggtgaagc gtttctacga ggctcgcggc tggcaggccg
     6781 tctggaccgg cgagcgggcc gaggagctca acgcggcgct gggcgacgcg gtgcgccatg
     6841 cgatcgacgc caagtcattc gtcgagcatg ccaccaaggg cgcaagcgcc gccgaacggg
     6901 aggcggggct gactctggcg gcgatcgaat atggccaggc cctcgccacc ggggccgtcg
     6961 atccgcgcaa gatctacgag gtctatacgg tgccgatgcc gaaggcggac gtggtcggcg
     7021 gcctcggacg ggcggtggac cagggcgggg tgcgccaatg gctcgccggc ctcgcgccgc
     7081 aggacaccga atataaggcg ctttccgacg cctatgtcgc ctaccgccag aaggccgcgc
     7141 aggagcagcg ggcggcggtc gcggccggcg agcagatcaa gccgggcaaa tcggacccgc
     7201 gcatcccgca gattcgccag gggctccagg ccaatggcta tctgcaggcc gccgcctcgg
     7261 cgccttcggg agactcgaag gccaaggcca aggcgacggc ggttcgcgcc gacgggaccg
     7321 tctattcggc cgacatggtc gccgcggtga agaaggtgca gaccgattac gggatcgagc
     7381 cggacggcgt gatcggcaat tcgaccctcg aggcgctcaa caccggcgcc agggaccggg
     7441 cccgaatcct cgcggtcaat ctcgagcggc ggcgctggct ggagcggact ccgccggcga
     7501 cccggatcga cgtcaacacg gcggcggcgg agctccatta ctggaaggac ggcagcgaga
     7561 aggaccggcg ccgcgtcgtc gtcggccagc ccgactggga gactcccgag ctcggctcgc
     7621 cgatcttccg gctggtcgcc aatccgccgt ggacggtgcc cgagtcgatc gccgaggagg
     7681 agatcctgcc caagggcgcc gcctacatgg cgaaagagaa tatcgtcatg aagaacggcc
     7741 ggctcgtcca ggagcccggc ccgaaatcgg cgctcggcct ggtcaagttc gacatgcaga
     7801 acgacgagca gatctacctt cacgacacgc cggccaaggc cctgttcgcc tcgacccagc
     7861 gccatgccag ccacggctgc tcgcgggtcg agaatgccgt cgagttcgcg cggctgctcg
     7921 cgcaggacga cggcaagctg gaggaattcg acaaggcgct cgcgaccaag aaggagaccg
     7981 cggtcgagct gtccagcaag atcccggtgc ggctgatgta ccacagcgcc tatctggccg
     8041 gcgggcgggt cgtcttccgc accgatccct acggctggga cgacaagctc gccgccgcgc
     8101 tcggcctcgg cggccaggtc cggccgcggg tcaagaccca tgtggcggat gtcgggccct
     8161 aagtcttaat tcctccacgc gatttcgcgg ggagggggac cgcgcgaacc gcggtggagg
     8221 gattcttcga ttcggggaga acccctccac caggcttcgc ctggtccccc tccccggcaa
     8281 atgccgggga ggatcgcatg agagcatcct agctcgactg cttgtccttg gtgtctatcc
     8341 ggtcggcgcc ggttgccgcc ttcttctcgc cggcgatggc ctcgccggac cggcctcggt
     8401 cgcgctcgtg atccttgagc gtcgggttgc ggttgttgtt ggtgccctcg cccttgccgc
     8461 tgttgccgcg catcgattcg tctccttcgc gcgccgacaa cgacccggcc gcgattcttc
     8521 tcccatccct tggtcgatcc cccaaggccg ttcgtcggcg cttcgggcgc ttggcagaga
     8581 cttggcggcg ccgcgtcgaa gcggacgcgc ggccggccgg gcgaccggct aacccaatcc
     8641 tgcggcgcgg ggttcccgac cctgtttcat ccccaggcca gggggtctcg cgccgcaacc
     8701 ccctttcgaa gccaaggacg aaacccatgt tccaccgtac gctttccgcg ctcgccgccg
     8761 ccgccctgac ggtgggggcg ttcgccttca ccgcgccggc ccaggccgcc gctgccgacg
     8821 actcggtcac catcgcgctc gacggcctca atccggccga tcccgccgat gccgaccgga
     8881 tcgcgaggcg catccgcaac gccgcccgcg gcctgtgcgg atcgcagctg atccagccga
     8941 cccggctgat gcagcacgcc gccgcctgcg agaaggccgt cgtcgccgag gcgcggaccg
     9001 cggtcgagac cgccgccgcc cgtcagggcg gtccgttccg cctcaccctc cgctcgaatt
     9061 gagcctcggg gacagtcact cgcgactgtc cccgcccatc gctaacggcc ggcgaggcct
     9121 ttggcattgg cca
//
LOCUS       DASVQZ010000399         5501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_948262, whole genome shotgun sequence.
ACCESSION   DASVQZ010000399 DASVQZ010000000
VERSION     DASVQZ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948262"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1075
                     /gene="lipA"
                     /locus_tag="VF547_11270"
     CDS             119..1075
                     /gene="lipA"
                     /locus_tag="VF547_11270"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX8263443.1"
                     /translation="MSEADPILTPPAAPRQRKPDWIRVKAPTSAGFAETKRLMRSLNL
                     ATVCEEAACPNIGECWTKKHATVMILGDTCTRACAFCNVKTGMPRAVDPLEPEHVATA
                     AAELGLTHIVVTSVDRDDLPDGGAKQFVRVIEALRRNTPSTTIEILTPDFRNKPESAV
                     EAIVAARPDVYNHNLETVPRLYPTIRPGARYYASLRLLESVKRQDPTIFTKSGLMVGL
                     GEQRLEVHQVMDDMRSADVDFLTMGQYLQPTPRHAGVEEFVTPQAFSAYAAIARAKGF
                     LLVAASPLTRSSYHAGDDFAKMKAAREAETASRPGEGRDPRG"
     gene            1133..1663
                     /locus_tag="VF547_11275"
     CDS             1133..1663
                     /locus_tag="VF547_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX8263444.1"
                     /translation="MPRHTETRRMPYAPEQMFDLVADVKRYPEFLPWVAAVRVRSDSD
                     TLMIADLVVGFRAIKETFTSRVEKQRPERLKTDYIEGPLKYLQNSWAFKPDGEGGTEI
                     DFCVDFAFKSRIFESLAGQMFDRALRRMIGAFEDRARELYGDLPSSPSPDGEGDRVKP
                     GGGVNPSESGGSRAIL"
     gene            complement(1692..2246)
                     /locus_tag="VF547_11280"
     CDS             complement(1692..2246)
                     /locus_tag="VF547_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX8263445.1"
                     /translation="MSEEREFCLPDELIEAARRVVEANRAAGLRLAVAESCTGGLVAA
                     ALTEIPGSSDVLEAGFVSYANESKVGLLGVSEDIVETFGAVSIAVAWAMAQGALKRTG
                     ADTAVAVTGIAGPGGGTEKKPVGTVIFARARRGADPKEIVADTHYFGDLGRGGVRMQA
                     VLCALDLLMPVSSSPSEDGEGDQA"
     gene            complement(2243..3409)
                     /locus_tag="VF547_11285"
     CDS             complement(2243..3409)
                     /locus_tag="VF547_11285"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX8263446.1"
                     /translation="MADAGKTVALIVAAGSGSRAGGDVPKQYRLVAGKTVLRRAVEPF
                     LDHPGIDAVRVVIGEGQQRLASAALAGIDVGELVPGGPERAFSVIRGLEALTPDTGRV
                     LVHDAARPFCPGAVISRLLEALEHHEGAVPVLRTADTLTRVTRDGLMGDHVDRDAVVR
                     VQTPQAFALDTLVQAIREWRGAPPTDEAAFLRAKRIAVGLVEGDPLLEKLTYDDDFAL
                     AEARLTGRMSSRTGIGFDVHAFAPGQALWLGGILIPHDRGLSGHSDADVVLHAVTDAL
                     LGAIGEGDIGEHFPPSDPQWRGAASSRFVEHARGLVEQRGGRIDHVDVTLICEAPRIG
                     PHRAAMRGRIAALLGLGESRVSVKATTTERLGFTGRGEGIAAQAVATVRLPEES"
     gene            3474..4472
                     /gene="dusB"
                     /locus_tag="VF547_11290"
     CDS             3474..4472
                     /gene="dusB"
                     /locus_tag="VF547_11290"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX8263447.1"
                     /translation="MSRLAPISIGPVRIDAPVILAPMTGVTDRPFRKAVKRYGAGLTV
                     TEMIASQAAIRETRQALQKADWDPIEDPVSMQLAGCTPAEMAEAAKLNEDRGAAIIDI
                     NMGCPVRKVVNGDAGSALMRDLGLAARIIDSTVKAVKVPVTLKMRMGWDHSSLNAPEL
                     ARIAVDLGVRMITVHGRTRCQMYKGEADWAFVRRVKEAVAVPVIVNGDICSIDDVAAA
                     LDQSGADGVMIGRGAYGRPWLLGQAMHWLRTGERLPDPGLDEQFAVLIDQYRSMLDHY
                     GPEVGVNVARKHVGWYTRGLHGSAEFRNAFNQQGDAKRAQAMLYDFYAPWLSRAAA"
     gene            4563..>5501
                     /locus_tag="VF547_11295"
     CDS             4563..>5501
                     /locus_tag="VF547_11295"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX8263448.1"
                     /translation="MNAEAPIQVESRPRWRRRLLVLFRRSRIVPFLEAGAVLALIVMA
                     VISYFVVAAQARSETLLTPPMVALLLVANLVPAMALMVLVARRVAMKRAALSPIGGRG
                     RLHVRLAAVFSLIASVPTLMVVVFASLLFQSGVEFWFSNRARGMIENAALLGQQAYAE
                     ELNRVNQESVTMSGDLAAYLREVPIDSPEFAEGFGRQVYQRNLSEAAVLKAAPGQEIQ
                     TIGVVNPYDRDLQKVVSQPMIAKLRGGEESVVIQGTDRIGAVTPLAFGNDFYLYAARV
                     HDPQMVAQLQRARGVLGDYTALVKRARVLQLRFNAAL"
BASE COUNT          841 a         1970 c         1860 g          830 t
ORIGIN      
        1 ggaacccggc gacctggctt ggggatttac gaaccgaccg tacaggagtg tcgctccggc
       61 cgaggccctg attagccggc ttgttgttaa gacgggccgg catcgctatc tggccggcat
      121 gagcgaagcc gatccgatcc tgactccgcc ggccgcgccg cgccagcgca agcccgactg
      181 gatccgggtg aaggccccga ccagcgcggg cttcgccgag accaagcggc tgatgcgcag
      241 cctcaacctc gccaccgtct gcgaggaggc ggcctgcccg aatatcggcg agtgctggac
      301 gaagaagcac gcgacggtga tgatcttggg cgacacctgc acccgcgcct gcgccttctg
      361 caacgtcaag accggcatgc cgagggcggt cgacccgctc gagcccgagc atgtcgcgac
      421 cgccgcggcg gagctcggcc tcacccatat cgtcgtcacc tcggtcgacc gggacgacct
      481 gccggacggc ggcgcgaagc agttcgtccg cgtgatcgag gcgctccggc gcaacacgcc
      541 ctcgacgacg atcgagatcc tcacgcccga cttccgcaac aagccggaga gcgcggtcga
      601 ggcgatcgtg gccgcccggc ccgacgtcta caaccacaat ctggagacgg tgccgaggct
      661 ctatccgacg atccgccccg gggcccgtta ctacgcgtcc cttcgcctgc tcgagagcgt
      721 caagcggcag gacccgacga tcttcaccaa gtcggggctg atggtcggcc tcggcgagca
      781 gcggctcgag gtccaccagg tgatggacga catgcgctcg gccgacgtcg atttcctgac
      841 catgggccag tatctccagc cgaccccgcg ccacgccggg gtcgaggagt tcgtcacgcc
      901 ccaggccttc tccgcctatg ccgccatcgc cagggcgaaa ggcttcctgc tcgtcgccgc
      961 gagcccgctg acccgctcca gctaccatgc cggcgacgac ttcgcgaaga tgaaggccgc
     1021 gagggaggcg gagacagcct cccgtcccgg cgaaggccgg gaccccaggg ggtgacgagc
     1081 tcggcgccag ccgtcgcccg ggcaccggcc tgcgccgggg cgacggactg acatgccccg
     1141 ccacaccgag acccggcgca tgccctacgc gcccgagcag atgttcgacc tcgtcgccga
     1201 cgtgaagcgc tatcccgaat tcctcccctg ggtggcggcc gtgcgggtgc gctcggacag
     1261 cgacacgctg atgatcgccg acctcgtcgt cggcttccgg gcgatcaagg agaccttcac
     1321 ctcgcgggtc gagaagcagc ggccggagag gctcaagacc gactatatcg aggggccgct
     1381 caaatatctc cagaacagct gggcgttcaa gccggacggg gaggggggca ccgagatcga
     1441 cttctgcgtc gacttcgcct tcaagagccg gatcttcgag agcctggcgg gccagatgtt
     1501 cgaccgtgcg ctgcggcgga tgatcggcgc gttcgaggac cgcgcaaggg agctttacgg
     1561 cgatctccca tcctccccat cgccagatgg ggagggggac cgggtgaaac ctggtggagg
     1621 ggtaaatccc tccgaatcag gcggttcgcg cgcgatcctc tgacgaatcc gcccgttacc
     1681 cctccaccaa gctacgcttg gtccccctcc ccatcttccg atggggagga cgagaccggc
     1741 atcagcaggt cgagcgcgca gagcaccgcc tgcatccgaa ccccgccgcg gccgaggtcg
     1801 ccgaaataat gggtgtcggc gacgatctcc ttggggtcgg cgccgcgccg ggcgcgggcg
     1861 aagatcaccg tgccgaccgg cttcttctcg gttcccccgc ccgggccggc gatgcctgtg
     1921 acggcgacgg cggtatcggc gccggtccgc ttcagggcgc cctgggccat cgcccaggcg
     1981 acggcgatcg acaccgcgcc gaacgtctcg acgatgtcct cgctcactcc gagcaggccg
     2041 accttcgatt cgttggcgta gctgacgaag cccgcctcga gcacgtcgga cgagcccgga
     2101 atctcggtca gcgcggcggc gacgagcccg cccgtgcagc tttccgccac cgccagcctc
     2161 aggcccgcgg cgcgattggc ctcgacgact ctcctcgccg cctcgatcag ctcgtcgggc
     2221 aggcagaatt cgcgctcttc gctcatgatt cctccggcag ccggacggtg gcgacggcct
     2281 gggcggcgat gccctcgccg cgcccggtga acccgagccg ctcggtcgtc gtcgccttga
     2341 cgctgacccg ggactcgccg agcccgagca gggccgcgat ccggccgcgc atcgccgccc
     2401 ggtgcgggcc gatcctcggc gcctcgcaga tcagggtgac gtcgacatgg tcgatgcggc
     2461 cgccgcgttg ctcgaccagg ccgcgggcat gctcgacgaa gcgcgacgag gcggcgcctc
     2521 gccattgcgg gtcggacggc gggaaatgct cgccgatgtc gccctcgccg atcgctccga
     2581 gcagggcgtc ggtgaccgcg tggaggacga cgtcggcgtc gctgtggccg ctgaggccgc
     2641 ggtcgtgggg aataaggatg cccccgagcc acagcgcctg gcccggggcg aaggcgtgga
     2701 cgtcgaagcc gattccggtc cggctgctca ttcgtccggt caagcgagcc tccgcgaggg
     2761 cgaaatcgtc atcataggtc agcttctcga gcagcggatc gccctcgacc agccccaccg
     2821 ctatccgctt ggcccgaagg aaggcggctt cgtcggtggg cggggccccc cgccactccc
     2881 ggatggcctg aacgagcgtg tcgagcgcga aggcctgcgg ggtctgcact cggaccaccg
     2941 cgtcgcgatc gacatggtcg cccatcaggc cgtcccgggt caccctcgtc agggtgtccg
     3001 ccgtgcgcag cacgggtacg gcgccttcat gatgctccag cgcctcgagg agccgcgaga
     3061 tgacggcgcc agggcagaac ggacgcgccg cgtcgtggac caggacgcgc cccgtgtccg
     3121 gcgtcagggc ctcgagcccg cggatcaccg aaaaagcccg ctccggcccg ccggggacga
     3181 gctcgccgac gtcgatcccg gcgagcgcgg ccgaggcgag acgctgctgg ccttcgccga
     3241 tcacgacccg gacggcgtcg attccgggat ggtcgaggaa cggctccacc gcgcgccgga
     3301 gcacggtctt gccggcgacg aggcgatatt gcttcggcac gtccccgccc gcgcggctcc
     3361 cggatccggc ggcgacgatc agggcgacgg tctttccggc atcggccatg tcgcgcgcct
     3421 agcccagcta cgccttgccc gccagcggct ttgcccctat ctgaccgcct cccatgtccc
     3481 gcctcgctcc catctccatc ggtcccgtcc ggatcgacgc gcccgtcatc ctcgcgccga
     3541 tgaccggggt gaccgaccgc ccgttccgca aggcggtgaa gcgctacggc gccgggctca
     3601 ccgtcaccga gatgatcgcc agccaggcgg cgatccgcga gacccgccag gccctccaga
     3661 aagccgactg ggaccccatc gaggatcccg tctcgatgca gctcgccggc tgcacgcccg
     3721 cggagatggc cgaggcggcg aagctcaacg aggaccgcgg cgccgccatc atcgacatca
     3781 acatgggctg cccggtccgc aaggtggtca atggcgacgc cggctccgcg ctcatgcgcg
     3841 acctcggtct ggcggcgcgg atcatcgatt cgaccgtgaa ggcggtgaag gtgccggtga
     3901 cgctcaagat gcggatgggc tgggaccatt cgagcctcaa cgcgcccgag ctcgctcgaa
     3961 tcgcggtcga tctcggcgtt cggatgatca ccgtccacgg ccgcacccgg tgccagatgt
     4021 acaagggcga ggcggattgg gcgttcgtcc ggcgggtcaa ggaggcggtg gcggtgccgg
     4081 tgatcgtcaa cggcgacatc tgctcgatcg acgacgtcgc cgccgcgctc gaccagtcgg
     4141 gggccgacgg cgtgatgatc ggccgcggcg cctatggccg gccgtggctg ctcggccagg
     4201 cgatgcactg gcttcgaacc ggcgagcgcc tgcccgatcc gggcctcgac gagcagttcg
     4261 ccgtcctgat cgaccaatat cgctcgatgc tcgaccatta cggcccggag gtcggagtca
     4321 acgtcgcgcg caagcatgtc ggctggtaca cgaggggcct gcacggctcg gcggagttcc
     4381 gcaacgcgtt caaccagcag ggcgacgcca agcgggccca ggcgatgctc tacgacttct
     4441 acgcgccctg gctcagccgc gccgcggcct gagccgattg ccccgtcacg gtgcggccag
     4501 acgagccgct ggcgccacat actgtgttct ttttaccaca ggcttgtggt agcctgatcc
     4561 gagtgaacgc cgaagctccc atccaagtcg agtccagacc gcgctggcgg cgccggctgc
     4621 tggtgctttt ccggcgcagc cggatcgtcc ccttcctcga ggccggggcg gtgctcgccc
     4681 tcatcgtcat ggccgttatc agctacttcg tggtcgccgc tcaggcgcgg tccgagactc
     4741 tgctgacccc gccgatggtc gccttgctcc tcgtcgccaa cctggtgccg gcgatggcgc
     4801 tgatggtgct ggtcgcccgc cgcgtcgcga tgaaacgcgc ggcgctgtcg ccgatcgggg
     4861 ggaggggacg gctccacgtc cggctggccg cggtcttctc gctgatcgcg agcgtgccga
     4921 ctctgatggt cgtggtgttc gcctcgctgc tgttccagtc gggcgtcgag ttctggttct
     4981 cgaaccgggc gcgcgggatg atcgagaacg ccgcgctgct cgggcagcag gcctatgccg
     5041 aagagctcaa ccgggtgaac caggaatcgg tgacgatgag cggcgacctc gccgcctatc
     5101 tgcgcgaggt gccgatcgac agccccgaat tcgccgaagg cttcgggcgc caggtctatc
     5161 agcgcaacct ctccgaagcc gccgtcctca aggccgcgcc cggacaggag atccagacca
     5221 tcggcgtcgt caatccttac gaccgcgatc tccagaaggt cgtctcgcag cccatgatcg
     5281 ccaagcttcg aggcggcgag gagagcgtcg tcatccaggg caccgaccgg atcggcgccg
     5341 tcactccgct cgccttcggc aatgatttct acctctatgc ggcgcgcgtc cacgacccgc
     5401 agatggtcgc gcagctgcag cgcgcccggg gcgtgctcgg cgattacacc gcgctcgtca
     5461 aacgggcccg ggtcctgcag ctgcgcttca acgccgcctt g
//
LOCUS       DASVQZ010000400         9522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_182347, whole genome shotgun sequence.
ACCESSION   DASVQZ010000400 DASVQZ010000000
VERSION     DASVQZ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182347"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..1513
                     /gene="bla"
                     /locus_tag="VF547_11300"
     CDS             329..1513
                     /gene="bla"
                     /locus_tag="VF547_11300"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX8263449.1"
                     /translation="MTASRNRVEGRSGRFARGAALAGIAAIGLATAALDGASAQPGAA
                     APSFPSTVRGYTTPGVPAATSQEATARRILESQVRALGSAFNGDIGIAVKDVQSGWTT
                     SFDGDTFFPQQSVSKFWVALTALDKSDRGQLSLDAPVTVRRDDMTLFHQPIAAQLGAG
                     GYNTTLNSLIYRAITQSDNTCNDFVLWKAGGPDAVRSFLRDRGLEGIRFGPGERLLQS
                     QIAGIQWQQSYAYNGGFYRARNAVPLAQRRAAFERYIANPMDGATPNGIVNALARLKR
                     GELLSPASTQKLLSIMANTKTGAQRLKGGLAPGWQLSHKTGTGQVLGGEQAGYNDIGI
                     LTGPDGRSYAVAVMIRRTSAPLGQRMAAMQSTVRAVIGFDQNLASYGLARYGAASEQG
                     SR"
     gene            complement(1543..2619)
                     /gene="paaE"
                     /locus_tag="VF547_11305"
     CDS             complement(1543..2619)
                     /gene="paaE"
                     /locus_tag="VF547_11305"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEX8263450.1"
                     /translation="MSAHFHSLAIAETVDETAEAKSIRFDVPPALRDRFRFKPGQHLT
                     LKAEIGGEEVRRNYSLCVAPADGELKVTVKRIAGGLFSNWANDHLKPGDTIDVMEPHG
                     SFTWDFDPKAANHYAAFAGGSGITPVMSLLKTALIEEPGSRFTLFYGNRDSQSVIFLD
                     ELARLKNRFMDRLEIHHFLAEEAEDIDLFNGMLDRGKCEEVLEHLIDPAHVAAFFICG
                     PGPMMDAAEAALRARGVASDKIHVERFTADRPPEALQAQLDALSRDAQGATMLVTLDG
                     RRRRVPFSAEAGNILDSARLAGLPAPFACKAGVCATCRARVVSGEVEMAARYGLTDEE
                     VAAGYVLTCQSVPKGEGLELDYDA"
     gene            complement(2630..3085)
                     /gene="paaD"
                     /locus_tag="VF547_11310"
     CDS             complement(2630..3085)
                     /gene="paaD"
                     /locus_tag="VF547_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX8263451.1"
                     /translation="MVAAARFDETGPIWAVLAEVPDPEIPVVSVVDLGIVRSVEPGRV
                     VVTPTYTGCPATQVIERDIRAALDSAGYRDVRIETVLAPAWTTDWITPEGRRKLHAYG
                     IAPPTRGAVQCPQCGSAETEEISRFGSTPCKSLWRCRCCAEPFDLFKCH"
     gene            complement(3079..3906)
                     /gene="paaC"
                     /locus_tag="VF547_11315"
     CDS             complement(3079..3906)
                     /gene="paaC"
                     /locus_tag="VF547_11315"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX8263452.1"
                     /translation="MASLPPIDLPSSATGAGAFDEPALDEGPALFEYLLRLGDDALIL
                     GQRLGEWCGHAPSVEVDLSLANIALDLIGQATHFLGRAGEVEGKGRDGDALAFRRDVL
                     DFRNCLLVEQPNGDFARTMARQLLYSTWQQLLFRNLLASKDALVAAVAEKAVKEVTYH
                     QELAAEWVIRLGDGTEESRRRIEDGLAWNWRFVPELFEMDDLARLMAERGIGADPTAF
                     RAEYDARVRAVLAEATLEPPPDQRPILGGRRGHHSEHLGHLLCTMQFLPRTYPDATW"
     gene            complement(4055..4336)
                     /gene="paaB"
                     /locus_tag="VF547_11320"
     CDS             complement(4055..4336)
                     /gene="paaB"
                     /locus_tag="VF547_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX8263453.1"
                     /translation="MNDWPLWEVFVRSKGGMAHRHVGSVHAPDAEIALRHARDTYTRR
                     MEGVSLWVVPSASIVASDPGQDEAMFDPAADKVYRHPTFYDIPEGITHI"
     gene            complement(4473..5456)
                     /gene="paaA"
                     /locus_tag="VF547_11325"
     CDS             complement(4473..5456)
                     /gene="paaA"
                     /locus_tag="VF547_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX8263454.1"
                     /translation="MYTTELAKTKNVASTEDPDKLAAFEARVAADEFIEPKDWMPEAY
                     RRTLTRQISQHAHSEIVGMLPEGNWITRAPSLRRKAILLAKVQDEAGHGLYLYSAAET
                     LGTSRREMIEALHSGKAKYSTIFNYPTLTWADIGAIGWLVDGAAIMNQVPLQRTSYGP
                     YARAMVRVCKEESFHQRQGYEIMMVMAAGTDDQKRMAQDALNRWWWPSLMMFGPPDAD
                     SPNTAQSMRWRIKRDTNDELRQRFVDITVPQADALGLKVPDPALKWNEARGHYDFGEV
                     DWEEFYAVVRGEGPVARERMTARRQAWEDGAWVREAAAAHAEKHSRKAEAA"
     gene            5780..6247
                     /locus_tag="VF547_11330"
     CDS             5780..6247
                     /locus_tag="VF547_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEX8263455.1"
                     /translation="MTFRVTGLSPEPFRALFGLADEELARRGALRVVADSPFGFPDRI
                     EISDAAPGESLLLLNYLHQPADTPYRSGHAIFVREREVAPTDLVGEIPPAMRARPLSL
                     RAFDGGDMMVDADLVDGAEAEALIERLLGDERVAYIQAHYARRGCYAGRIERA"
     gene            complement(6349..7551)
                     /gene="pcaF"
                     /locus_tag="VF547_11335"
     CDS             complement(6349..7551)
                     /gene="pcaF"
                     /locus_tag="VF547_11335"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX8263456.1"
                     /translation="MTEAFICDAVRTPIGRYGGSLAHVRADDLAAVPIRALIDRNPGV
                     DWGELDDVILGCANQAGEDNRNLARMAALLAGLPDRSGGVTLNRLCGSGMDAVAMAAR
                     VIRGSEADMVIAGGSESMSRAPFVMAKAQSAFDRNAEMYDTTIGWRFVNPKMKHAYGD
                     DTMPSTAENVAQEWQVSREDQDAFAVRSQDKAAAAQDNGRLGAEIVPVAIPQRKGDPL
                     VVDRDEHPRKTSVEALAKLRPIVRSDGTVTAGNASGVNDGAAAMVVASEEAAKRNGLT
                     PRARFVGGAVAGVPPRIMGIGPAPASQKLLQRLGLSVSDMDVIEINEAFAAQALPVLR
                     ELGLPDDAEHVNPNGGAIALGHPLGMSGARLLLTAAEELHRRGGRYALCAMCIGVGQG
                     IATVIERV"
     gene            complement(7698..8207)
                     /locus_tag="VF547_11340"
     CDS             complement(7698..8207)
                     /locus_tag="VF547_11340"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX8263457.1"
                     /translation="MRGESDEVKRCFPPVVDSGTLLLVLGSLPGEESLARGQYYANPR
                     NHFWRLIGAVAGLELVPLGYEARLAALLAAGVGLWDMVGSATRRGSLDGAIRDPDAND
                     LAALAARLPRLRAVGFNGGKSASLGVPQLAGRPDLALIPLPSSSPAYTRPFEEKLAEW
                     MKLAPFLNG"
     gene            8368..>9522
                     /locus_tag="VF547_11345"
     CDS             8368..>9522
                     /locus_tag="VF547_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263458.1"
                     /translation="MVAIFSGSGLGYQRGSASVLGSAGSLGEAAQGRGGDNVMVNAAT
                     GNLIVSRKDEFLVGRGPDATYAQTYNSQGDPTNSWNASVYHVLTDIPATANVAGSTIT
                     RGDGDGHSSVYTYDVAKGAYVGTEGGGAHDELRYANGAWTWTDGDSRTRETYAALTGV
                     AGQQFLASVVDADGNTQSYSWNPNGTLQKITNANGEYVQFTLGANGLPTQVTTYSSAG
                     AALLTRTRYAWDASRRLTSVTVDLTPGDHSVADGKSYTTTYTYDGTSSRIASISQTDG
                     SKLDIAYVLVGADYRVATLTQTVATGSTRTTSFSYDTVNRITAVTDGNGQVTSLYHDA
                     ARQLTRITAPPAYSGAPANSVQFGYNASGDLVSVTDGNNAAATYSYDSSGN"
BASE COUNT         1492 a         3259 c         3329 g         1442 t
ORIGIN      
        1 cgccatgcga gcttcctcaa ggacgtggcg cccctcgcgg gccgcgacat caggctgacc
       61 gcccgcgtca acttctgatc ggggaattcc tccccggctt ttgccgggga gggggaccag
      121 gcgaagcctg gtggaggggt ccttccggcg ccgaagaacc ccaccaccgc gcttcgcgcg
      181 gtccccctcc ccgagaaatc tcggggagga attgaagctc cgtcgcaagg gcagaacggc
      241 tcatcgtttg gccgccgctt ttaagctggc ggaaagcccc cgacccgtac catgattcca
      301 ttgagggata agggcttagg aggggcaagt gacggcatcg cggaatcgcg tcgagggaag
      361 atcgggccgt tttgcgcgcg gcgccgcgct cgcgggcatc gccgccatcg gcctcgccac
      421 cgccgcgctc gacggcgcct cggcccagcc cggagcggcc gcgccctcct tcccctcgac
      481 cgtccgcggc tacaccacgc ccggagtccc cgccgcgact tcgcaggaag cgaccgcgcg
      541 ccggatcctc gagagccagg tgcgagcgct cggaagcgcg ttcaacggcg acatcggcat
      601 cgcggtcaag gacgtgcaga gcggctggac gaccagcttc gacggcgaca ccttcttccc
      661 gcagcagagc gtcagcaaat tctgggtggc cctgaccgcc ctggacaagt ccgaccgcgg
      721 ccagctcagc ctcgacgcgc cggtcaccgt ccgccgcgac gacatgaccc tcttccacca
      781 gccgatcgcc gcccagctcg gcgccggcgg ctacaatacg acactcaaca gcctgatcta
      841 ccgggcgatc acccagagcg acaatacctg caacgacttc gtgctgtgga aggcgggcgg
      901 gcccgacgcc gtccggagct tcctccgcga ccgcggcctc gaggggatcc gcttcggccc
      961 gggcgagcgg ctgctccaga gccagatcgc cggcatccag tggcagcaga gctatgccta
     1021 caatggcggc ttctaccggg cccgcaacgc ggtgccgctg gcgcagcgcc gggccgcgtt
     1081 cgagcgctac atcgccaacc cgatggacgg ggcgaccccc aacgggatcg tcaacgcgct
     1141 cgcccgcctg aagcgcggcg agctgctgtc gccggcctcc acccagaagc tgctctcgat
     1201 catggccaac accaagaccg gggcgcagag gctcaagggc ggactcgcgc cgggctggca
     1261 gctctcgcac aagaccggca ccggccaggt gctcggcggc gagcaggccg gctacaacga
     1321 catcggcatc ctcaccggcc cggacggccg ctcctacgcg gtggcggtga tgatccgccg
     1381 cacctcggct ccgctgggcc agcggatggc ggcgatgcag agcacggtga gagcggtgat
     1441 cggcttcgac cagaatctgg ccagctacgg cctggcgaga tacggcgcgg cctcggagca
     1501 ggggagccgg tagcaccatt cctccccgtt ccggggagga acttaagcgt cgtagtccag
     1561 ctccagccct tcgcccttgg gcacggactg gcaggtgagg acgtagcccg ccgccacctc
     1621 ctcgtcggtg aggccgtagc gggcggccat ctcgacctcg cccgacacca cccgggcccg
     1681 gcaggtggcg caaaccccgg ccttgcaggc gaagggcgcg gggaggccgg cgaggcgggc
     1741 gctgtcgagg atgttgccgg cctcggcgct gaacggcacc cgccgcctgc ggccgtcgag
     1801 cgtgaccagc atggtcgcgc cctgagcgtc cctggacagg gcgtcgagct gggcctggag
     1861 cgcctcgggc ggccggtcgg cggtgaagcg ctcgacatgg atcttgtccg aggcgacgcc
     1921 gcgcgcgcgc agggccgcct cggcggcgtc catcatcggc ccgggtccgc agatgaagaa
     1981 ggcggcgaca tgggcgggat cgatgagatg ctcgagcacc tcctcgcatt tgccgcggtc
     2041 gagcatgccg ttgaacaggt cgatatcctc ggcctcctcg gcgaggaaat ggtggatctc
     2101 gagccggtcc atgaagcggt tcttgaggcg cgccagctcg tctaggaaga tcaccgactg
     2161 gctgtcgcga ttgccgtaga agagggtgaa gcggctccca ggctcctcga tcagcgcggt
     2221 cttgagcagc gacatcaccg gcgtgatgcc cgagccgccg gcgaaggcgg catagtgatt
     2281 ggccgccttc gggtcgaaat cccaggtgaa gctgccgtgc ggctccatca cgtcgatcgt
     2341 atcgcccggc ttcagatggt cgttggccca gttggagaag aggccgccgg cgatgcgctt
     2401 gacggtcacc ttgagctcgc cgtccgccgg ggcgacgcag agcgagtagt tgcggcgcac
     2461 ctcctcgccg ccgatctccg ccttcagggt gagatgctgg cccggcttga acctgaaccg
     2521 atcgcggagc gccggcggaa cgtcgaagcg gatcgatttg gcctcggcgg tctcgtcgac
     2581 cgtctcggcg atggcgaggc tgtggaaatg cgcgctcatc cgccgcgcct caatggcatt
     2641 tgaacaggtc gaacggctcc gcgcagcacc ggcagcgcca gagcgacttg cagggcgtcg
     2701 agccgaagcg gctgatctcc tcggtctcgg ccgagccgca ttgcggacat tggaccgccc
     2761 cccgagtcgg tggggcaatg ccgtaagcgt gaagcttgcg gcggccttcc ggggtgatcc
     2821 agtcggtggt ccaggcgggg gcgagcacgg tctcgatgcg gacgtcgcgg tagccggcgg
     2881 agtcgagcgc ggcgcgtatg tcgcgctcga tcacctgggt ggcggggcag ccggtatagg
     2941 ttggcgtgac gacgacccgg ccgggctcga ccgagcggac gatgccgagg tcgacgaccg
     3001 agaccaccgg aatctcggga tcgggcacct cggccaggac ggcccagatc ggcccggtct
     3061 cgtcgaaacg ggcggcggct accatgtcgc gtccggatag gtgcggggga ggaactgcat
     3121 cgtgcacagc aggtggccga gatgctcgct gtggtggcct cggcggccgc cgaggatcgg
     3181 gcgctggtcg ggcggcggct cgagcgtcgc ctcggcgagg acggcgcgga cgcgggcgtc
     3241 gtactcggcg cggaaggccg tgggatcggc gccgatgccg cgctcggcca tcagcctggc
     3301 gagatcgtcc atctcgaaca gctcgggcac gaagcgccag ttccaggcga gcccgtcctc
     3361 gatccgccgc cggctctcct cggtgccgtc gccgaggcgg atcacccatt cggccgccag
     3421 ctcctggtgg taggtgacct ccttcaccgc cttttccgcg accgcagcga cgagcgcatc
     3481 cttcgaggcg agcaggttcc ggaagagcag ctgctgccag gtcgagtaga ggagctgacg
     3541 ggccatggtg cgcgcgaagt cgccattggg ctgctcgacc aggaggcagt tgcggaagtc
     3601 gagcacgtcg cggcggaagg cgagcgcgtc gccgtcgcgg cccttgcctt cgacctcgcc
     3661 ggcgcggccc aggaaatggg tcgcctggcc gatcaggtcg agcgcgatgt tggcgaggct
     3721 gaggtcgacc tcgacggacg gggcgtggcc gcaccattcg ccgagccgct ggccgaggat
     3781 cagcgcatcg tcgccgaggc ggaggagata ttcgaacagg gcagggcctt cgtccagcgc
     3841 gggctcgtcg aaggcgccgg cgccggtggc gctcgagggg aggtcgatgg gggggaggga
     3901 ggccattagc gaaattcctc ccctgcattt gcaggggagg gggaccaggc gcagcctggt
     3961 ggaggggcct tccggcgcca tagaacccct ccaccgcgct tcgcgcggtc cccctccccg
     4021 agaaatctcg gggaggaatt taagcgcacc cccctcatat gtgggtgatc ccctccggga
     4081 tgtcgtagaa ggtcgggtgg cggtagacct tgtcggcggc gggatcgaac atcgcctcgt
     4141 cctggcccgg atcggaggcg acgatcgagg ccgaggggac gacccacagg ctgaccccct
     4201 ccatccgccg cgtgtaggtg tcgcgggcgt ggcgcagcgc gatctccgcg tcgggcgcgt
     4261 ggaccgagcc gacgtggcgg tgggccatgc cgcccttcga gcggacgaag acctcccaga
     4321 gcggccagtc gttcattggc gcagatccgc cgtacgtcct tcgagacgcg gcttcgacaa
     4381 gctcagccgc tcctcaggat gagcggtggg taattgatat cgctcatcct gaggagggac
     4441 tgagcccggc gaaggcccgt ctcgaaggac cgtcatgccg cttccgcctt cctcgaatgc
     4501 ttctccgcat gggcggcggc cgcctcgcgg acccaggcgc cgtcctccca ggcctggcgc
     4561 cgggccgtca tccgctccct ggcgaccggg ccctcgccgc ggacgacggc gtagaactcc
     4621 tcccagtcga cctcgccgaa atcgtaatgg ccgcgcgcct cgttccactt cagcgccgga
     4681 tcggggacct tcaggccgag cgcgtcggcc tgggggacgg tgatgtcgac gaagcgctgg
     4741 cgaagctcgt cgttggtgtc gcgcttgatc cgccagcgca tcgactgggc ggtgttgggg
     4801 ctgtcggcgt cgggcgggcc gaacatcatc agcgacggcc accaccagcg gttcaaggcg
     4861 tcctgggcca tgcgcttctg gtcgtccgtg ccggccgcca tcaccatcat gatctcatag
     4921 ccctggcgct ggtggaagct ctcctccttg cagacccgga ccatcgcccg ggcgtagggg
     4981 ccgtaggagg tgcgctggag cggcacctgg ttcatgatcg cggcgccgtc gaccagccag
     5041 ccgatcgcgc cgatgtccgc ccaggtcagc gtcgggtagt tgaagatggt gctgtacttg
     5101 gccttgccgg aatggagcgc ctcgatcatc tcgcggcggc tggtgccgag ggtctcggcg
     5161 gcggaataga ggtagaggcc gtggccggcc tcgtcctgga ccttggcgag caggatcgcc
     5221 ttgcggcgca gcgagggcgc ccgggtgatc caattgcctt cggggagcat gccgacgatc
     5281 tccgaatgcg catgctgcga gatctggcgg gtgagggtgc ggcggtaggc ctccggcatc
     5341 cagtccttgg gctcgatgaa ctcgtcggcc gcgacccgcg cctcgaaggc ggcgagcttg
     5401 tcgggatcct cggtcgaggc gacgttcttc gtcttggcta gctcggtcgt gtacattcca
     5461 gtctcccggc gcggccctag cagggggcac cattcaccgc aaacaacgcg cgaatcggtc
     5521 gcgggtggcg gagcgagtcg caagccgatt cggcgggcaa agcttgcgaa acttacacat
     5581 gccctcggtt tcggccgaca ctgtcagctt cgcacctcgg agaggcggac gcggagcgga
     5641 aaagcgaagc cgggcatgag cggaacatag cacaaacatt ggcgcttgcg ggggattttt
     5701 cggactttgg cggcaagcgg cggagcgtcg gggcgccggc ccctgcgcca tgattggggc
     5761 gcaacgccag gaggaagcga tgacattccg agtgaccggc ctttcgcccg aaccgttccg
     5821 cgccctgttc ggccttgccg acgaggagct tgcccgccgc ggcgccctcc gggtcgtcgc
     5881 cgactcgccg ttcggattcc ccgaccggat cgagatcagc gacgccgcgc cgggcgagag
     5941 cctgctgctg ctcaactatc tgcaccagcc ggccgacacg ccctatcgct cgggccacgc
     6001 catcttcgtg cgggagcgcg aggtggcgcc gaccgacctc gtcggcgaga tcccgccggc
     6061 gatgcgggcc cggccgctgt cgctgagggc gttcgacggc ggggacatga tggtggacgc
     6121 cgacctggtc gacggcgccg aggccgaggc cctgatcgag cggctgctgg gcgatgagcg
     6181 ggtcgcctac attcaggcgc attacgcccg gcgcggctgc tatgccgggc ggatcgagcg
     6241 ggcttgaacc gtcattgcga gcgaagcgaa gcaatccaga gccgcgcctg gcgccaccgc
     6301 cgcgctggat tgccgcccgg cgttcgccgg ggtcctcgca atgacgacct agacccgctc
     6361 gatcaccgtg gcgatgccct ggccgactcc gatgcacatg gcgcacaaag catagcggcc
     6421 gccgcggcgg tggagctcct cggcggcggt gaggaggaga cgggcgccgg acatcccgag
     6481 cgggtggccc agggcaatgg ccccgccgtt cgggttgacg tgttccgcgt cgtcggggag
     6541 gccgagctcg cggagcaccg ggagcgcctg ggcggcgaag gcctcgttga tctcgattac
     6601 atccatatca gagacactta gaccgagacg ttgcaacagc ttctgagagg ccggcgccgg
     6661 gccgatgccc atgatccgag gcgggacccc ggcgacggcg ccgccgacga agcgggcgcg
     6721 cggggtcagg ccgttgcgct tcgccgcctc ctcggacgcg accaccatcg ccgcggcgcc
     6781 gtcattgacc ccggaggcgt tgcccgcggt gaccgtcccg tccgaacgga cgatcggcct
     6841 cagcttggcc agcgcctcga cgctggtctt gcggggatgc tcgtcgcgat cgacgacgag
     6901 cggatcgccc ttgcgctgcg ggatcgcgac cgggacgatc tccgcgccga gccggccatt
     6961 gtcctgggcc gccgccgcct tgtcctggct tcgaaccgcg aaagcatcct gatcctcgcg
     7021 acttacctgc cattcctgag caacgttctc agcagtggag ggcatggtgt cgtcgccata
     7081 ggcatgcttc atcttcgggt tgacgaagcg ccagccgatc gtcgtgtcgt acatctcggc
     7141 attccggtcg aacgcgctct gcgccttggc catgacgaac ggggcgcggc tcatgctctc
     7201 cgagccgccg gcgatcacca tgtccgcctc gcttccgcgg atgacccgcg cggccatggc
     7261 gaccgcgtcc attcccgacc cgcacaggcg gttgagcgtg actccgccgg accggtcggg
     7321 gaggccggcg agcagggcgg ccatgcgcgc gaggttgcga ttgtcctcgc cggcctggtt
     7381 ggcgcagccg aggatgacgt cgtcgagctc gccccagtcg acgcccggat tgcggtcgat
     7441 cagggcgcgg atcgggacgg cggcgagatc gtcggcgcgg acgtgagcga ggctgccgcc
     7501 gtagcggccg atcggggttc gcaccgcgtc gcagatgaag gcttcggtca tatccgggat
     7561 ccgctcgagg gtttgatggc cagcccccta gccgttcggc ccgagcttgt cgaaggccct
     7621 tccttgcctt cccgtgccgc gagttgaagc ggagggcttc gacaggctca gcccgaacgg
     7681 gagtgaattt cgttcggtca gccgttgaga aagggcgcga gcttcatcca ctccgccagt
     7741 ttctcctcga aaggccgggt ataggccggg ctgctcgacg gcagcggaat caaggcgagg
     7801 tccggcctcc cggccagctg cggcacgccg agcgaggctg acttgccgcc gttgaagcca
     7861 accgcccgaa gccgggggag gcgcgcggcg agggcggcga gatcgttggc gtcggggtcg
     7921 cggatggcgc cgtcgaggct gccgcgccgg gtcgcgctcc cgaccatgtc ccacaggccg
     7981 actccggcgg cgagaagcgc ggcgaggcgg gcctcatagc ccaagggcac cagctcgagg
     8041 ccggcgaccg ccccgatcag gcgccagaaa tggtttcgcg gattggcgta atattggcct
     8101 cgggcgagcg attcctcgcc gggcaggctg cccaggacga gcagcagcgt gccggagtcg
     8161 acgaccggcg ggaagcagcg tttcacctcg tcagattcgc cacgcaagcc ggtcgattag
     8221 ccgtttttgc ctttccaggc cattcgcctt gtgcccgggc cgccgcggtc cgcgcggtcg
     8281 attaatagct aaacatttcc ctgcctcctc cgatcaacag tgcaaagggg aggggcatgt
     8341 ggccgacccc gtcagggggg ttgaagaatg gtcgcgatct tttccggcag cggtctcggt
     8401 taccagcgcg ggtcggcgtc ggtgctgggg tcggcgggct cgctcggcga ggcggcgcag
     8461 gggcgcggcg gcgacaatgt catggtcaac gcggccaccg gcaacctgat cgtcagccgc
     8521 aaggacgagt tcctcgtcgg ccgcggtccc gatgcgacct atgcccagac ctacaacagc
     8581 cagggcgatc cgaccaattc ctggaacgcg agcgtctacc atgtgctcac cgacatcccg
     8641 gccacggcca atgtcgccgg gagcacgatc acccgcggcg acggcgacgg ccacagctcg
     8701 gtctacacct acgatgtggc gaagggcgcc tatgtcggca ccgagggcgg cggcgcccat
     8761 gacgagctgc gctacgccaa cggcgcctgg acctggaccg acggcgacag ccgcacccgc
     8821 gagacctatg ccgcgctcac cggagtggcc ggccaacagt tcctcgccag cgtcgtcgac
     8881 gccgacggca acacccaatc ctacagctgg aacccgaacg ggaccctcca gaagatcacc
     8941 aacgccaatg gcgaatatgt ccagttcacg ctcggcgcca acggcctgcc cacccaggtc
     9001 acgacctaca gctcggccgg cgcggcgctg ctcacccgca cccgctacgc ctgggacgcc
     9061 tcgcgccggc tgaccagcgt caccgtcgac ctcacccccg gcgaccattc ggtggccgac
     9121 ggcaagagct acaccaccac ctacacctac gacggcacct cgtcgcggat cgcgtcgatc
     9181 agccagaccg acggctccaa gctcgacatc gcctatgtcc tggtcggcgc cgactaccgc
     9241 gtggcgactc tcacccagac ggtggcgacc ggctcgacgc gcacgaccag cttctcctac
     9301 gacaccgtca accggatcac cgcggtcacc gacggtaacg gccaggtcac ctccctctat
     9361 cacgacgcgg cccggcagct caccaggatc accgcgcctc cggcctattc cggagcgccg
     9421 gcgaacagcg tccagttcgg ctacaatgca agcggtgacc tcgtctcggt gaccgacggc
     9481 aacaacgccg ccgccaccta cagctacgat tcgtcgggca at
//
LOCUS       DASVQZ010000401         4308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_948646, whole genome shotgun sequence.
ACCESSION   DASVQZ010000401 DASVQZ010000000
VERSION     DASVQZ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948646"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VF547_11350"
     CDS             <1..613
                     /locus_tag="VF547_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8263459.1"
                     /translation="KAQLAGVSMAEVPATLSKDGRSRPPHLRSWRDGWRHLKFLLLFA
                     PRSLYVYPGLALFALSFAAFLGLLPGDLTVGSVRFGVHSLLFAGAGMLVGAQMMSFGL
                     VASQFGVRERYWSAGRGLDRVRSLLTIDKGSLIGGAMITAGLGGAVVALASWAGAGFS
                     DMDPDMLMRLSIPSVLSAGIGLQLMLTSFLLELLSHPARYPQR"
     gene            618..1226
                     /locus_tag="VF547_11355"
     CDS             618..1226
                     /locus_tag="VF547_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8263460.1"
                     /translation="MSLFKRLHSTAHDKAVFSRRIEVLTRTLADFIPPNARVLDIGCG
                     SGTLAKRIMELRPDVTIEGIDVLVRPGTEIPVTEFDGDTIPWPDGHFDIALFVDVLHH
                     TEAPARLLAEAKRVSKGGIVIKDHFREGVLADATLRFMDWVGNAQHGVVLPYNYLSDP
                     EWRGIWSKLGLKVERLTDKVGLYPAPFSWLFDRRLHFVARLA"
     gene            1354..2901
                     /locus_tag="VF547_11360"
     CDS             1354..2901
                     /locus_tag="VF547_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263461.1"
                     /translation="MATLIHTLPHAKTPLSQRLLARPTLCFWLAGILIYGLAAWHFSG
                     GHLTNQTSRDLWQHLAALRALIENPFAPANPFVTTAEGSRHFHPYWVSVALVAKTLGW
                     DPWQAIAFAGYVSAGVLLAGIHTFGRAFYRTPWGPLALLAAMAFGWCLPISHTGYHSL
                     GTLIEGIAYPAALLIGLSLLLWGLVIRALERPARIWLIVPLSAFMFATHQLGAGIGFL
                     AAGCFILFWPEATLKARAGAAAAIAAGMVLAMAWPYHDPFEAIARTGLPTWTGGVDFY
                     VPMMLANAFIPSLLGLAGLLHPRVRRTALPALAAFALFAGIFVLGKFGILIATRFVMP
                     AVLMLHIGLGALFLVTAAKWRDFRHGTQLGLFTLGMLSLTSFVFFTWVSLSVEMKGYR
                     RTGNSYLHAEALTRDIPDTEAVAAYDVFAWPIVATGQRVVSVPWPEPMIDGLAERQAA
                     AERLFDPALTRDERLRLARQWGARTLVLNSNGPLRRPMPKDMLKRLKEQSVRWYRSGP
                     FVRFDLE"
     gene            complement(2986..3411)
                     /locus_tag="VF547_11365"
     CDS             complement(2986..3411)
                     /locus_tag="VF547_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8263462.1"
                     /translation="MNDDQLRDVASSLHGAAIRLLRMVRREDRQTGISPPRLSALSVL
                     VFAGPLSLAELAAAEDVRAPTMSRIVEGLVRDGLASREADPGNRRKVRIAATEEGRRR
                     LEAGRERRVRLLAEQLRRLADSEQRALARGVEVLERAVR"
     gene            3490..3849
                     /locus_tag="VF547_11370"
     CDS             3490..3849
                     /locus_tag="VF547_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8263463.1"
                     /translation="MSPTRLAQVAITVEDLERAKAFYRDTLGLTHLFDAPPGLAFFQC
                     GETRLMLSRPEGPEGAASSILYYGFDDVDAAHQAMTARGVVFEEAPRKIAEVDGKPIH
                     LAICRDGEGNLLGLISG"
     gene            3965..>4308
                     /locus_tag="VF547_11375"
     CDS             3965..>4308
                     /locus_tag="VF547_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263464.1"
                     /translation="MGTKTWCAAAAALIAAAPLEAAELLDGNRVEQRSGAFAGATLRI
                     GLDGTRERPSTPRLGFGVSRVQLRSSSAARIDRVQTPGLELALAAGRPRLLVGGESAE
                     AARTRLGMSSTTT"
BASE COUNT          585 a         1600 c         1404 g          719 t
ORIGIN      
        1 caaggcgcag ctcgccggag tctcgatggc cgaggttccc gccacgctga gcaaggacgg
       61 ccgctcgcgc ccgccgcacc tcaggagctg gcgcgacggc tggcgccacc tcaagttcct
      121 gctgctgttc gcgccgcgct cgctctacgt ctatccgggg ctggccctgt tcgccttgag
      181 cttcgccgcc ttcctgggcc ttctgcccgg cgacctgacc gtcggcagcg tccgcttcgg
      241 agtccactcg ctgctcttcg ccggcgccgg aatgctggtc ggagcgcaga tgatgagctt
      301 cggcctcgtc gccagccagt tcggcgtccg cgagcgctat tggtccgccg gccgagggct
      361 cgaccgggtc cggtccctgc tcacgatcga caagggctcg ctgatcggcg gagcgatgat
      421 caccgccggc ctcggcggag cggtcgtcgc cctcgcctcc tgggccggcg ccggcttcag
      481 cgacatggat ccggacatgc tgatgcgcct gtccatcccc tccgtcctct ccgcgggcat
      541 cggcctgcag ctcatgctga cctccttcct gctcgagctg ctctcccatc ccgcccgcta
      601 cccgcaaagg tgaagccatg agcctgttca aacgcctcca cagcacggcc cacgacaaag
      661 cggtgttcag ccgccggatc gaggtcctga cgcgcaccct cgccgatttc atcccgccca
      721 acgcccgcgt gctggacatc ggctgcggca gcggcacgct ggcgaagcgg atcatggagc
      781 ttcggcccga cgtcaccatc gaagggatcg acgtcctcgt ccgccccggc accgaaatcc
      841 cggtgaccga gttcgacggc gacaccattc cctggcccga cggccatttc gacatcgctt
      901 tgttcgtcga cgtcctccac cataccgagg cgccggcgcg gctgctggcg gaggcgaagc
      961 gggtctcgaa gggcgggatc gtcatcaagg accatttccg cgaaggcgtc ctcgccgacg
     1021 cgacgctgcg cttcatggac tgggtcggca acgcccagca cggcgtcgtg cttccctaca
     1081 actacctctc cgatccggag tggcgcggca tctggtcgaa gctcggcctc aaggtcgagc
     1141 ggctgaccga caaggtcggc ctctatccgg cgccgttcag ctggctgttc gaccgccggc
     1201 tccatttcgt cgcccggctc gcctagggcc gatgccgcgg cgagccttgc cgtttcccgg
     1261 cgaaggccgg gatccagtcg agcgcgtgcc gccggacccc ggctatcgcc ggggaacggg
     1321 ctctcaacct caaggaacgg ttccggacta gagatggcca ccctgatcca cactctcccg
     1381 cacgcgaaga cgccgctgtc gcagcggctg ctcgcccggc ccacgctctg cttctggctg
     1441 gcggggatcc tgatctacgg cctcgccgcc tggcatttca gcggcggtca tctgaccaac
     1501 cagacgtcgc gcgacctgtg gcagcatctg gcggcgcttc gggcgctgat cgagaatccc
     1561 ttcgccccgg cgaacccgtt cgtgacgacg gccgagggct cgcgccattt ccacccttat
     1621 tgggtgtcgg tcgcgctcgt cgccaagacg ctgggatggg atccgtggca ggccatcgcc
     1681 ttcgccggct acgtcagcgc cggcgtcctg ctcgccggga tccacacctt cggccgagcc
     1741 ttctatcgca cgccctgggg gccgctcgcg ctgctggcgg cgatggcgtt cggctggtgc
     1801 cttccgatca gccacaccgg ctatcattcg ctgggcaccc tgatcgaggg cattgcctat
     1861 ccggccgccc tcctgatcgg actgtcgctg ctgctgtggg ggctggttat ccgggcgctc
     1921 gaacgcccgg cccggatctg gctgatcgtg ccgctgtccg ccttcatgtt cgcgacccat
     1981 cagctcggcg ccggcatcgg cttcctggcc gcgggctgct tcatcctgtt ctggcccgaa
     2041 gccaccctca aggcccgggc cggcgccgcc gccgccatcg ccgccggcat ggtgctcgcc
     2101 atggcctggc cctatcatga cccgttcgag gcgatcgccc gcaccggcct tccgacctgg
     2161 accgggggag tcgatttcta cgtgccgatg atgctggcca acgccttcat cccctcgctc
     2221 ctcggcctgg ctgggctgct ccatccgcgc gtccgccgga ccgccctgcc cgcgctcgcc
     2281 gccttcgccc tgttcgcggg catcttcgtc ctcggcaagt tcgggatcct catcgccacc
     2341 cgcttcgtca tgccggcggt gctgatgctc catatcggtc tcggcgcgct cttcctcgtc
     2401 accgcggcca aatggcggga cttccgccac ggcacccagc tcggcctctt caccctcggc
     2461 atgctgtcgc tgaccagctt cgtcttcttc acctgggtct cgctcagcgt cgagatgaag
     2521 ggctacaggc ggaccggcaa ttcctacctc catgcggagg cgctcacccg cgacataccg
     2581 gacaccgagg cggtcgcggc ctacgacgtc ttcgcctggc cgatcgtcgc cacgggccag
     2641 cgggtcgtct ccgtgccgtg gccggagccg atgatcgacg ggctcgccga gcgccaggcc
     2701 gccgccgagc ggctgttcga tccggccctg acccgcgacg agcgccttcg gctcgcgcgg
     2761 caatggggcg ctcggaccct ggtcctcaac agcaacggcc cgctgcgccg gccgatgccg
     2821 aaggacatgc tgaagcgcct gaaggagcag tcggtgcggt ggtaccggtc cggcccgttc
     2881 gtccgcttcg acctggaata gcgggtcgag gcaggctcgg ttggaagagc ccgagccgtt
     2941 tgggccgggc ctgcccaagc ctctcgcgcc gcctgctccc tcgacctagc ggaccgcccg
     3001 ctcgaggacc tccaccccgc gggccagggc cctttgctcg ctgtcggcca gccgccgaag
     3061 ctgctcggcg aggaggcgca cacgccgctc gcggccggcc tcgagccgcc gccgtccctc
     3121 ctccgtcgcg gcgatccgga cctttcggcg gttgccgggg tcggcctcgc ggctggccag
     3181 cccgtccctc accaggccct cgacgatccg cgacatggtc ggcgccctga cgtcctccgc
     3241 cgcggcgagc tcggcgagcg acaaggggcc ggcgaagacc agaaccgaca aggccgacag
     3301 gcgcggcggg ctgattccgg tctggcgatc ctcgcgccgg accatccgca gcagccggat
     3361 cgccgccccg tgcaggctgc tcgcgacgtc gcggagctga tcgtcgttca acctcttgcc
     3421 tccgattgtt agctaggcta acggttgctt cggtacaaca gttagcatgg ctgcctaagg
     3481 agaggaagcg tgagcccgac ccgccttgct caagttgcga tcaccgtcga ggacctggag
     3541 cgcgccaagg cgttctaccg cgacacgctc ggcctgaccc acctgttcga cgccccgccc
     3601 ggccttgcct tcttccagtg cggcgagacc cggctgatgc tgagccgccc cgaaggtccg
     3661 gaaggcgcgg cgagctcgat cctctattac ggcttcgacg acgtcgacgc cgcccatcag
     3721 gcgatgaccg cgagaggcgt cgtcttcgaa gaggcgccgc gcaagatcgc cgaggtcgac
     3781 ggcaagccca tccacctcgc catctgccgc gacggcgaag gcaatctcct cggcctgatc
     3841 agcggttgag gcggatcccg ccgatgagtt cgagtcgtcc gcgcctcgtg ctagaagata
     3901 gccaggcgcg ggaatcgccg agccgaagtc cgattggcca catggacgaa cggggagagt
     3961 cgctatgggt acgaagacgt ggtgcgccgc cgcggcggca ctgattgcgg cggcgccgct
     4021 cgaagccgcc gagctgttgg acggcaaccg ggtcgagcaa cgcagcggtg ccttcgcggg
     4081 cgccacgctg aggatcgggc tcgacggcac acgcgagcgg ccgtcgacgc cgcggctcgg
     4141 cttcggcgtc agccgggtcc agttgcgcag ctcctcggcg gcgcggatcg accgggtgca
     4201 gacgcccgga ctcgagctgg cgctggcggc cggcaggccg cggctcctgg tcggcggcga
     4261 gagtgccgaa gcggcgcgaa cgcggctcgg aatgagcagc acgacgac
//
LOCUS       DASVQZ010000402         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_81771, whole genome shotgun sequence.
ACCESSION   DASVQZ010000402 DASVQZ010000000
VERSION     DASVQZ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81771"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VF547_11380"
     CDS             complement(<1..1216)
                     /locus_tag="VF547_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8263465.1"
                     /translation="MQVARGRLSMFVLLAGADAAEDGQALGRMAGALRGCGGTARLWR
                     DPRRAAAAAALKPAFVPEDAFDSQPLASEAGVFVCEARLDNRGELLEQLGFPADAEIA
                     DSTLLAAAYDRWGAECVERIVGDYAFAAWHRRDGRIVAAVDPLGMRRLMWARVGGGIA
                     LSPQIPALLANRRVSRDPDLHALAKVLDAGLDRNSTAFALIRALPGGHRLVWTGGEPQ
                     IDRWWRPGFGADLWYDDPRDYVEEARQLFTDAVRAQLRSSSPISSTLSGGLDSGSVTA
                     AAARLLSDRGGQLTAYTSVPETGLTPSQRYNWEPDDSGYAAEVAAGLGNVDHRLVAPE
                     GRCTLDVSRSLVERSRTPAKTATNLLWADAIAARMAASGSRVLLVGQRGNHAFSWRGQ
                     NTVWELAMLGKAG"
     gene            1265..1525
                     /locus_tag="VF547_11385"
     CDS             1265..1525
                     /locus_tag="VF547_11385"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX8263466.1"
                     /translation="MLRREENVIVAEVDGKPRLLNLRSWTYLALNDTGERIWALLAEP
                     KDRAALLRRLADEFDAPAETIARDLDSFLGELREQGFLAPDQ"
     gene            complement(1519..>1705)
                     /locus_tag="VF547_11390"
     CDS             complement(1519..>1705)
                     /locus_tag="VF547_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263467.1"
                     /translation="WAWLRLWRGLPDSAVAAVDRNFERWRPLLAIPLLVADAGRAAAA
                     GLLRLAGRRGRARSHAH"
BASE COUNT          266 a          629 c          599 g          211 t
ORIGIN      
        1 cgcccgcctt cccgagcatc gcgagctccc acaccgtgtt ctgccctcgc cagctgaaag
       61 cgtggttgcc ccgttgcccg acgaggagca cgcgcgagcc ggaggcggcc atcctcgcgg
      121 cgatggcgtc ggcccacagc aggttcgttg ccgtcttggc cggcgtgcgc gagcgttcga
      181 caagcgaccg ggacacgtcg agcgtgcaac gtccctccgg cgccacgaga cggtggtcga
      241 cattgccgag tccggcagcg acttcggccg catatccgct gtcgtcgggc tcccaattat
      301 atcgctgcga gggcgtcagg cccgtctcgg gcaccgacgt ataggcggtc agctgcccgc
      361 cgcggtccga cagaaggcgg gcagctgcgg cggtgacgga accggaatcg aggccgccgc
      421 tcagagtcga ggagatcggg ctgctgctgc gcagctgagc ccgcaccgcg tcggtgaaca
      481 gctggcgggc ttcctccaca tagtcccgag ggtcgtcgta ccacaggtcc gctccgaacc
      541 ccggccgcca ccagcggtcg atctgcggct cgccgccggt ccagaccagc cggtggccgc
      601 ccggcagcgc ccgtatgagg gcgaaggccg tcgaattgcg gtcgaggccg gcatcgagga
      661 ccttggcgag cgcgtgaagg tccgggtcgc gcgatacccg ccggttcgcg aggagggcgg
      721 ggatctgcgg cgaaagggca atcccgccgc cgacccgagc ccacatgagc cgccgcatcc
      781 cgagcggatc gacggcggcg acgatccgtc cgtcccggcg atgccaggcg gcgaaggcat
      841 agtcgccgac gatccgctcg acgcactccg cgccccagcg atcgtaggcg gcggcgagca
      901 gcgtcgaatc ggcgatctcc gcatcggccg ggaagccgag ctgctcgagc agctcgccgc
      961 gattgtcgag ccgcgcctcg cagacgaaga ccccggcctc gctcgccagc ggctgcgagt
     1021 cgaaagcatc ttccggaacg aaggcgggct tcaaggctgc ggcggcggcg gctcgccggg
     1081 ggtcgcgcca aagccgcgcg gtgccgccgc agccccgaag cgcaccggcc atgcgtccca
     1141 gcgcctgccc atcctcggcc gcgtcagcgc cggccagaag cacgaacatg ctcagccggc
     1201 cccgcgcgac ttgcaacagc ggggctcacc ggccatcagg cccgccggag cgagagggag
     1261 cgcgatgctg cggcgcgaag agaatgtcat cgtcgccgag gtagacggca agccgaggct
     1321 gctcaacctg cgaagctgga cctatctcgc cctcaacgac accggcgagc gcatctgggc
     1381 gctgctcgcc gagccgaagg accgtgccgc cctgctccgc cgcctcgccg acgagttcga
     1441 cgcgcctgcc gagacgatcg ccagggacct cgattccttt ctcggcgagc ttcgcgaaca
     1501 gggattcctc gctccggatc agtgagcgtg ggatcgcgcc cggccgcgcc ggccggcgag
     1561 gcgcagcagg cccgccgccg ccgcccggcc ggcgtcggcg acaagcagcg gaatcgcgag
     1621 gagcggccgc cagcgctcga aattgcggtc caccgcggcg accgcgctgt cgggcagtcc
     1681 gcgccacagc ctcagccacg cccag
//
LOCUS       DASVQZ010000403         9688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_81942, whole genome shotgun sequence.
ACCESSION   DASVQZ010000403 DASVQZ010000000
VERSION     DASVQZ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81942"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..765)
                     /locus_tag="VF547_11395"
     CDS             complement(1..765)
                     /locus_tag="VF547_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX8263468.1"
                     /translation="MPIEKAIVLSAGKGSRLLPLTAERPKCLIELSGRSLLEWQLDAL
                     AQAGIRETVVVTGFRDDLVDAVAARREGVRTLFNPFYHVADNLGSVWMARAELDSDTL
                     LLNGDTLVSPALLATVLGARTGPIAVTVDEKESYDADDMKVLRDGDRLLRIGKALEPG
                     RYNAESIGLLAFRGEGPSLFAAQVDRMMRQPDGTRRWYLRAIDELAQGGGDVRTVSIK
                     GEEWQEVDFPEDVESARALTARWAALPRHSGEARKS"
     gene            1035..2810
                     /locus_tag="VF547_11400"
     CDS             1035..2810
                     /locus_tag="VF547_11400"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M10 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8263469.1"
                     /translation="MRLGGLGQDGIVLSDGIILTGADGITVTGTDGVTATGSDGITAT
                     GADGITMTGADGVLVGDLKPFLLEDFAGLATWAGKPIYNQQQVINQIDGGAAVAANNG
                     KITYTFLTAPTTTGLYNSPNYQELGITESFGYSPMSEAEMAVARQSMTLWDDLIPQTF
                     VEKNGLGADITLANTTTGPAQAHAYFPGNGPRILGDVWTADPSVNWTNAWLTYGGYGR
                     TTLIHEIGHSLGLSHPGDYNFGDDGPDPDTLPDPITYEGDAFYAQDTGQYTIMSYFRD
                     QKSGGQAINVSLALLNNPQTPMLHDILTIQSKYGVDPNTRSGDTVYFANSNAGNGVYD
                     LAQNPFPHLAVYDAGGEDTFDFSTANTGVFIDLRPGAFSSATRGFLPLADANAATAEF
                     NAVTDAAQGDFAMWTASSYNGWLNTVMAIGQNRVANDTGVTGVTATSHRNIAIAYNTI
                     IENANGGSARDYLFGNDVSNKLNGNGGNDVLDGHLGDDIYTGGAGADEFRVSQIGGNE
                     KITDFTSGVDKIRLSEIDADSGVAGNQAFTFIGNAAFSNTAGQLRTYSQGGDNYLAGD
                     VNGDGVADFTINLGSGAPVVTDIFL"
     gene            2971..3810
                     /locus_tag="VF547_11405"
     CDS             2971..3810
                     /locus_tag="VF547_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8263470.1"
                     /translation="MGDWTAIVLAGRRPGEDGFAASHGVAAKALIPAGGEPMLGRVAR
                     TLLESPSIGRIVVLAQDSPALLTGALGWMSEEPRIATARAGDGISTSVGALAGTAEAP
                     YPVLVTTADHPLLRPEMVEAFIAGAQGADGAFAMVERRTVEALHPATRRTWLKAADGH
                     YSGANLFALMTAASRLGTDFWARAEKDRKRTLKLLSYLGPGIFLRALTRTISLQAAAE
                     RAGRKVGMRLRAVVLPFAEAAIDVDKPADLELAERILAGRAESPAPSSSAAAAASGRA
                     AAP"
     gene            complement(3751..5688)
                     /locus_tag="VF547_11410"
     CDS             complement(3751..5688)
                     /locus_tag="VF547_11410"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX8263471.1"
                     /translation="MHGLLLALLGALALALPGAAAADERILHYLSDVAVAADGTLDVT
                     ETIRVRSEGASIDHGIYRDFPTRYTSRAGAQVRVGFEVVGVQRDGADEPWKTERQGNG
                     VRIYAGDADTHVPPGEHVYVIRYRTTRQLGFFKDHDELYWNATGNGWAFPIDVAEARI
                     RLPRPVPFGERAAYTGPQGSTARNAEVVSESPGEILFRTTAPLGPEEGLTVAAAWEKG
                     VVAPPPPPSAARLWLQDNGPPAAGVLGLLGLLAYYFHAWRRAGRDPKPGTIVPLFSPP
                     DGLSAAAMRYITKMGADSRAFAAALVELGVGGHIRLSVEEGGFFSRDKTTIEKTAGRT
                     PVPAPEAAMMSSLFAGGDSILMDQKNHSTFSTAQKALERNLKQLYEGRLFVRNQVWAV
                     RGLALMVAALSLPAAILLLSAPDDYGRGDLLVAAAALLLPAAALALYYLGRGWGSAMA
                     MFGKLLAGFIGVLGLVAGVVTVGNALETVFAEESFRRLVPLLLPLLALPVVLTAFWWM
                     AAPTREGRAVLDRIAGFRHYLSVAEEDRLEALHPPDKTPELFERYLPHAIALKVENDW
                     ASRFTSVLAAAAASGQAHHMGWYSGHSDPWNDPGGFADQVGSSLASTVSSASTAPGSS
                     SGSGGGGSSGGGGGGGGGGGW"
     gene            complement(5688..6251)
                     /locus_tag="VF547_11415"
     CDS             complement(5688..6251)
                     /locus_tag="VF547_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8263472.1"
                     /translation="MGLWIALGLLVLALLYAVIVYNRLVRLRALVREGFSGITVQLRR
                     RADLIPNLVSTVEGYATHEREVLTEVTAHRSDPTNAGSVRATAEADNAFGAMLGRLMA
                     VAEDYPDLKADDNFRQLQEELAEIEEELQGARRYYNATVRDLNTSVQSFPDVLIAGPM
                     GFSEEPFYADADSGIQNAPKVSFGRAG"
     gene            complement(6398..7042)
                     /locus_tag="VF547_11420"
     CDS             complement(6398..7042)
                     /locus_tag="VF547_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263473.1"
                     /translation="MSTVHTVRTWRAPPGGPAGRFRRRLTVAGTVAAALTALLAFPGA
                     VGRQAAAFCTAFAQPGLGDACAGLGVKGVPTRSERELWQSAGSGSCEALRRYLREYPD
                     GAFGAPAARRLAAARWQRSSRWKPFDRTTVGAMSLQQDPLPTEALARRDVLSRIPADA
                     APRGCRPVDGSERLVRVEVDFAEPSCTRALGGYLCSADYRATCKMAARELEETC"
     gene            complement(7078..9633)
                     /locus_tag="VF547_11425"
     CDS             complement(7078..9633)
                     /locus_tag="VF547_11425"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263474.1"
                     /translation="MFGIAGAALVAGWAAPSRAQAPNDPRRVAPLVAALLAQQDAAVI
                     RERRLADVRQLATIGRYEARVRTLAAQLHGLALRISGAESASRKERERRLQVEATLGE
                     ARRDLARLTESLSRRDSEWAVQRDAYRAEAENLVAQATPEKLEALERFANGDREGAWP
                     VLAELTQASVRARLAAAGLRAAAEIRQLASLRAVMRANGEATAADVLALWEQAAALDP
                     RHFWTQIEIAYLAEDSGKLERAGLAAQRADEIAGTDRERSYAARTLGDVLIAQGSLSA
                     ADTAYRRALDLSRRLAAASPGDAAAERDLALVISRVGDVEQRRGRLAEAIALFRESMV
                     LNRKRLAANPGDPKVRSDLAIDHERQGDTLIAQGDVKAALSSYRASLDLRREVVRANP
                     SSALHKADLAISLNRLGDALAELGKTDEALTSYRDAVRYSREIVRADPTNGRFSRDLS
                     AHLSSEGRALAAQGKVAEALSRYREAVAISRTLSAADPTSGAKLEQFYDGLTSLAGIY
                     MTQGDLAQAAAAYAEALALARRRALLDPSDVAAREGVSSVLDSLGTVLRYQGELGRAA
                     EHFREALAIDREAARIDPSSGGAQQNLVSGLSSLGSLLVYQGKPDEALPLLDEALAIQ
                     RRWIASDDSAMRLRAALVDTLLQVGEVHLVKGDLAAAQRINAEAETVARSILAVEPDH
                     VLGKSKLSFVLARKGRLARASQDYGGAEAAYREALQIRTALAELDGSDAVAQQELAGA
                     MYDVSLAFMEQDKPADAIRMLERALPITRRLARADKTDTSLARDEHLILRQLGANRGS
                     LGEYASAIATLEESLAIARRLSAASPDAVQFRKDVEATAALIEMVRSMSEGSK"
BASE COUNT         1506 a         3381 c         3407 g         1394 t
ORIGIN      
        1 tcaggacttc cgggcttcgc cggaatgacg ggggagggcg gcccaacgcg cggtcagggc
       61 ccttgccgac tcgacgtcct cggggaagtc gacttcctgc cattcctcgc ccttgatcga
      121 gacggtgcgg acgtcgccgc cgccttgggc aagctcgtcg atcgcgcgca gataccagcg
      181 gcgggtgccg tcgggctggc gcatcatccg gtcgacctga gccgcgaaca ggctcgggcc
      241 ctccccccgg aaggcgagca ggccgatcga ttcggcattg taccggcccg gctccagcgc
      301 cttgccgatg cgcagcagcc ggtcgccgtc gcgaagcacc ttcatgtcgt cggcgtcata
      361 ggactccttc tcgtcgacgg tgacggcgat cgggccggtc cgcgcgccga gcaccgtcgc
      421 gagcagggcg ggcgagacca gggtgtcgcc gttgaggagc agcgtgtcgg agtcgagctc
      481 ggcgcgggcc atccacaccg agccgagatt gtccgcgacg tggtagaagg ggttgaaaag
      541 cgtgcgcacc ccctcgcggc gggcggccac cgcgtcgacg agatcgtcgc ggaagccggt
      601 gacgacgacg gtctcgcgga tgccggcctg cgccagcgcg tcgagctgcc attcgagcag
      661 gctccggccg gagagctcga tcaggcattt cggccgctcc gcggtcagcg gcagcagccg
      721 cgagcccttg ccggcgctca ggacgatggc tttttcgatc ggcatcaggc gctcgtttcg
      781 ttgcagaaaa ggcattgcgc ccgccccctt ttacttcggc ggcccgagac cgccagcctg
      841 taccgttctt gcctgaatcg cagcgtcttc atttcggcta atttcgcgcc aaattctcgt
      901 taataatcaa ttgacagctc ccgatttccc gttgagcttc cccacttgcg ccggggggcc
      961 aaactcgttt gccaccgcgc atgctcaaca ctccattggg gggagaattc actgtggctg
     1021 ttcagaaact tagcttgcgt cttggcggtc tcggccagga cggcatcgtc ctttccgacg
     1081 gaatcatcct gaccggtgcg gacggaatca ccgtgaccgg caccgacgga gtcactgcga
     1141 ccggctccga cggaataacc gcgaccggcg ccgacggaat caccatgacc ggcgccgacg
     1201 gggtcctcgt cggcgacctg aagccgtttc ttctcgagga ctttgccggg ctcgcgacct
     1261 gggccggcaa gccgatctac aaccagcagc aggtgatcaa ccagatcgac ggcggcgccg
     1321 ccgttgccgc caacaacggc aagatcacct acaccttcct gaccgcgccc accacgacgg
     1381 gcctctacaa cagcccgaac taccaggagc tcggcatcac cgagagcttc ggctactcgc
     1441 cgatgagcga ggccgagatg gcggtcgcgc gccagtcgat gacgctgtgg gacgatctca
     1501 tcccgcagac cttcgtcgag aagaacggtc tcggcgccga catcacgctc gcgaacacga
     1561 cgaccggccc ggcccaggcc catgcctatt tccccggcaa cggtccgagg atcctcggcg
     1621 acgtctggac ggccgatccg agcgtcaact ggacgaacgc ctggctcacc tacggcggct
     1681 atggtcgaac gacgctgatc cacgagatcg gccacagcct cggcctcagc cacccgggcg
     1741 actataattt cggcgacgac gggcccgatc cggacaccct gcccgatccg atcacctatg
     1801 agggcgacgc cttctacgct caggacacgg gtcaatatac gatcatgtcc tatttccgcg
     1861 accagaagtc gggtggccag gcgatcaacg tgtcgctcgc tctgctcaac aacccgcaga
     1921 cgccgatgct tcacgacatc ctgacgatcc agtcgaaata tggcgtggat ccgaacacgc
     1981 gcagcggcga caccgtctat ttcgccaatt cgaacgccgg caacggggtc tacgacctcg
     2041 cccagaaccc gttcccgcat ctcgccgtct acgacgcggg cggcgaggac acgttcgact
     2101 tctcgacggc gaacaccggc gtgttcatcg acctgcgtcc cggcgcgttc agctcggcga
     2161 cccgcggctt cctgccgctg gccgacgcca atgcggcgac ggcggaattc aacgcggtga
     2221 ccgatgcggc ccagggcgac ttcgcgatgt ggaccgcgtc gagctacaac ggctggctca
     2281 acaccgtgat ggcgatcggc cagaaccggg tggcgaacga taccggcgtg accggcgtca
     2341 ccgccacctc gcaccgcaac atcgcgatcg cctacaacac gatcatcgag aatgcgaacg
     2401 gcggctcggc gcgcgactat ctgttcggga acgacgtctc caacaagctg aacggcaatg
     2461 gcggcaacga cgtgctcgac ggccatctcg gcgacgacat ctacaccggc ggcgccggcg
     2521 cggacgagtt ccgggtgagc cagatcggcg gcaacgagaa gatcaccgac ttcaccagcg
     2581 gagtcgacaa gatccggctg tccgagatcg acgcggacag cggagtcgcc ggcaaccagg
     2641 ccttcacctt catcggcaat gcggcgttca gcaacacggc cggccagctg aggacctatt
     2701 cgcagggcgg ggacaattac ctcgccggcg acgtcaacgg cgacggcgtc gccgacttca
     2761 cgatcaacct cggcagcggc gcgccggtgg tgacggacat cttcctctag gaagcggtcc
     2821 gccgggaaaa gggaagggcc ggccgcgccg cggcgggccc tttctttttg cggccgcggg
     2881 cgtggcggat cggcaacggc ggcgcgattt accgcgccgg gcgcattttt cgatggctcc
     2941 tgaggcctct agctgtgatt gactgcggcg atgggggatt ggaccgcaat cgtgctggcc
     3001 gggcgccggc ccggagagga tggcttcgcg gcctcccacg gagtcgccgc caaggcgctg
     3061 atcccggccg gcggcgagcc gatgctcggg cgggtggcgc gcaccctcct ggaatcgccg
     3121 tcgatcggcc ggatcgtcgt gctcgcccag gattcgccgg ccctgctgac cggcgcgctc
     3181 ggctggatga gcgaggagcc gcggatcgcg acggcccgcg ccggggacgg catctcgacc
     3241 agcgtcggcg cgctcgccgg gacggccgag gccccctatc ccgtgctggt gacgacggcc
     3301 gaccatccgc tgcttcgtcc ggagatggtc gaggccttca tcgccggcgc ccaaggcgcg
     3361 gacggcgcct tcgcgatggt cgagcgccgg acggtcgagg cgctccatcc ggccacccgg
     3421 cggacctggc tcaaggccgc cgacggccat tacagcggcg ccaatctgtt cgccctgatg
     3481 acggcggcct cgcggctggg gacggacttc tgggcgcggg cggaaaagga ccgcaagcgc
     3541 acgctgaagc tgctcagcta tctcggcccc ggcatcttcc tgcgcgcgct gacccggacg
     3601 atctcgctcc aggccgcggc cgagcgggcg ggccgaaagg tcgggatgcg gctgagggcc
     3661 gtcgtcctgc ccttcgccga agcggcgatc gacgtcgaca agccggccga cctggagctg
     3721 gccgagcgca tcctcgccgg ccgggcggag tcaccagccc cctcctcctc cgccgccgcc
     3781 gccgcctccg gacgagccgc cgccccctga tccgctgctc gagcccggcg cggtggaggc
     3841 cgaggacacc gtgctcgcca gcgaggagcc gacctggtcg gcgaagccgc cgggatcgtt
     3901 ccacggatcg gagtgcccgg aataccagcc catatgatgg gcctgccccg acgcggcggc
     3961 agcggcgagg accgaggtga agcgcgacgc ccagtcgttc tcgaccttga gcgcgatcgc
     4021 gtggggaaga tagcgctcga acaattcggg cgtcttgtcg ggcggatgaa gcgcctcgag
     4081 ccggtcctcc tcggcgaccg agagatagtg gcggaagccc gcgatccggt cgagcaccgc
     4141 ccggccttcg cgggtcggcg cggccatcca ccagaaggcg gtgagcacca ccggcaaggc
     4201 gaggagaggc aggagcaagg gcaccagccg gcggaaggac tcctcggcga agaccgtctc
     4261 gagcgcgttg ccgaccgtga cgacgccggc gacgaggccg agcaccccga tgaagccggc
     4321 gaggagcttg ccgaacatcg ccatggcgct gccccaaccg cggcccagat agtagagagc
     4381 cagggccgcc gccggcagaa gcagggcggc ggcggcgacg aggaggtcgc cgcgcccgta
     4441 gtcgtccggc gcggacagga gcaggatcgc ggcgggaagc gacagggccg ccaccatcag
     4501 cgccaggccg cgcaccgccc agacctggtt gcgcacgaag agccggcctt cgtagagctg
     4561 cttgagattc ctctccagcg ccttctgggc ggtgctgaag gtggagtggt tcttctggtc
     4621 catcaggatc gaatcgccgc cggcgaacag gctcgacatc atcgcggcct cgggcgcggg
     4681 caccggcgtc cggccggcgg tcttctcgat cgtcgtcttg tccctgctga aaaagccccc
     4741 ttcctcgacg ctgaggcgga tatggccgcc gaccccgagc tcgacgagag cggccgcgaa
     4801 ggcgcggctg tcggccccca tcttggtgat gtagcgcatc gccgccgccg acaggccgtc
     4861 gggcggcgag aagagcggaa cgatcgtccc gggcttcggg tcgcggccgg cccgccgcca
     4921 ggcgtggaaa taataagcga gcaggccgag cagcccgagc acgccggccg ccggagggcc
     4981 gttgtcctgg agccagagcc gcgcggccga cggcggcggc ggtggagcga cgacgccctt
     5041 ctcccaggcc gcggcgacgg tcagcccctc ctcaggcccg agcggggcgg tggtgcggaa
     5101 gaggatctcg ccgggactct cggagaccac ctcggcattg cgggcggtcg agccctgcgg
     5161 accggtatag gccgcccgct cgccgaaagg gaccggccgg ggcagccgga tccgggcctc
     5221 ggcgacgtcg atcgggaagg cccagccgtt gccggtggcg ttccagtaga gctcgtcatg
     5281 gtccttgaag aagccgagct gacgcgtcgt ccggtagcgg atgacgtaga catgctcgcc
     5341 cggaggaacg tgggtatcgg cgtcgccggc atagatccgg acgccattgc cctggcgctc
     5401 ggtcttccag ggctcgtcgg cgccgtcgcg ctggaccccg acgacctcga agccgacccg
     5461 gacctgggcg ccggcgcggc tggtgtagcg cgtcgggaag tcgcggtaga tgccgtggtc
     5521 gatcgaggcg ccctcggacc gcacccggat cgtctcggtg acgtcgagcg tgccgtcggc
     5581 cgcgaccgcg acgtcgctca gataatggag gatccgctcg tccgccgcgg cggcgccggg
     5641 aagagcgagg gcgagcgccc cgagcagcgc cagcaggagg ccgtgcatca gccggcccgg
     5701 ccgaagctga ccttgggcgc gttctggatg cccgaatcgg cgtcggcgta gaacggctcc
     5761 tccgaaaagc ccatcggccc ggcgatcagc acgtcgggga aggactggac gctggtgttg
     5821 aggtcgcgga cggtcgcgtt gtaatagcgc cgcgcgccct gcagctcctc ctcgatctcg
     5881 gcgagctcct cctggagctg gcggaaattg tcgtcggcct tgaggtccgg ataatcctcg
     5941 gccacggcca tcagccggcc gagcatggcc ccgaacgcgt tgtccgcctc ggcggtggcc
     6001 ctgaccgagc ccgcgttggt cggatcgctt cgatgggcgg tgacctcggt gagcacctcg
     6061 cgctcatggg tcgcatagcc ttcgaccgtg ctgaccagat tggggatgag atcggcgcgg
     6121 cggcgaagct ggacggtgat tccgctgaag ccttcgcgga cgagagcgcg caggcggacc
     6181 aggcggttgt agacgatgac ggcgtagaga agggccagaa cgagcaggcc gagcgcgatc
     6241 cataatccca tggcgatccc ccttgccccg ccatctctag gacaggcggc cccccggggc
     6301 tacccacccg gcgcgggttg gtccatccga agaccggaag cgggccgtgg atgccggatc
     6361 gaggccggga cggcgatcaa gccgattgcc gaatgcccta gcaggtctcc tcaagctccc
     6421 gcgcggccat cttgcaggtg gcgcggtagt cggccgagca aaggtagccg ccgagggccc
     6481 gggtgcagga cggctcggca aaatcgacct cgacccgaac cagccgctcc gatccgtcga
     6541 ccgggcggca cccgcgcgga gccgcatccg cgggtatccg cgacagcaca tcccgccgcg
     6601 ccagcgcttc ggtcggcagc gggtcctgct ggagagacat ggcgccgacg gtggtgcggt
     6661 cgaacggctt ccaccggctc gagcgctgcc atcgcgcggc cgcgagccgc cgcgccgccg
     6721 gcgcgccgaa cgcgccgtcg ggatactccc ggaggtaccg gcgcagtgcc tcgcaggagc
     6781 cgctccccgc cgactgccac agctcgcgtt ccgagcgcgt cgggacgccc ttcacgccca
     6841 gtccggcgca ggcgtcgccg aggcccggct gagcgaatgc cgtgcagaag gcggcggcct
     6901 gccggcctac cgcgccggga aatgcgagga gcgccgtcag agcggcggca acggtcccgg
     6961 ccaccgtgag gcggcgcctg aagcggcccg cgggcccgcc cggcggcgct cgccaggtgc
     7021 gcacggtgtg gacagtcgac atgcgtgccc agcgcgccgc agccctggcg aaccggatca
     7081 cttgctcccc tcgctcatcg accggaccat ttcgatcagc gcggcggtcg cctccacgtc
     7141 cttccggaac tggaccgcgt ccggactcgc cgcggagagg cgccgcgcga tggcgagact
     7201 ctcctcgagg gtggcgatcg cactggcata ttccccgagc gaaccccggt tggcccccaa
     7261 ttgccggagg atgagatgct cgtcgcgagc cagggacgta tcggtcttgt cggctcgagc
     7321 gaggcgacgg gtaatcggca gcgcgcgctc cagcatcctt atggcgtcgg cgggcttgtc
     7381 ctgctccatg aaggcgaggg agacgtcgta catggcgccg gcgagctcct gctgcgcgac
     7441 ggcgtcggaa ccgtccagct cggccagggc cgtgcggatc tggagcgcct cgcgataggc
     7501 ggcctccgcc ccgccataat cctgcgaggc acgggccagg cggcccttgc gggccaggac
     7561 gaaagacagc ttcgactttc ccaggacatg gtccggctcg accgccagta tcgagcgcgc
     7621 caccgtctcg gcttcggcat tgatgcgctg ggcggcggcc agatcgccct tcacgagatg
     7681 cacttcgccg acctggagca gcgtgtcgac cagggcggcg cgcagcctca tggcgctgtc
     7741 gtcgctggcg atccatctgc gctggatggc gagcgcctcg tcgagtagcg gcaatgcctc
     7801 gtcgggcttg ccctggtaga cgagcaggct gccaagcgag gacaggcccg acaccaggtt
     7861 ctgctgggcg ccgccgctcg acggatcgat ccttgcggct tcgcggtcga tggcgagagc
     7921 ctcgcggaaa tgctcggccg cccggccgag ctcgccctgg tagcgcagca ccgtgccaag
     7981 gctgtcgagg acggagctga cgccttcgcg cgccgccaca tcggagggat ccaggagggc
     8041 ccggcgccgc gccagggcca gcgcctcggc ataggcggcc gcggcctggg cgaggtcgcc
     8101 ctgagtcatg tagatgcccg cgaggctcgt caggccgtcg tagaattgct ccagcttggc
     8161 gccgctggtc gggtcggccg cgctcagcgt tcgggaaatc gccacggcct cccggtaccg
     8221 ggacaatgcc tcggccacct tgccctgggc cgcgagggcg cgcccctcgc tggacaaatg
     8281 ggccgacaga tcgcgcgaga agcggccgtt ggtcggatcg gcacgaacga tctcacggga
     8341 atagcgcacg gcgtcgcggt agctcgtgag ggcctcgtcg gttttgccaa gctcggcgag
     8401 cgcgtcgccg agccggttca gggagatcgc gagatcggcc ttgtggagcg ccgacgaggg
     8461 gtttgcgcgc acgacctcgc ggcgcagatc gaggctggcc cggtagctcg aaagcgcggc
     8521 tttgacgtcg ccctgggcga tgagggtgtc gccctgccgc tcgtgatcga tggcgagatc
     8581 gctccggacc ttgggatccc cgggattggc cgccagacgc ttccggttca acaccatcga
     8641 ctcccggaac aaggcgatcg cctcggcgag cctgcctcgc cgctgctcga cgtccccgac
     8701 ccgggatatg accaaggcga ggtccctctc ggcggcggcg tcgccgggac tggcggcggc
     8761 aagccggcgg gacagatcga gggcgcgccg ataggcggtg tcggccgctg aaaggctgcc
     8821 ctgggcgatc agcacgtcgc ccaaggtccg cgccgcatag gagcgctccc ggtcggtgcc
     8881 ggcgatctcg tccgcgcgct gcgccgcgag cccggcgcgc tcgagctttc cggaatcctc
     8941 ggcgagatag gcgatttcga tctgggtcca gaaatggcgc gggtcgagcg ccgccgcctg
     9001 ctcccacaag gcgagcacgt cggcggcggt tgcctcccca ttggcgcgca tgacggctcg
     9061 gagcgaggcg agctgccgga tctcggccgc ggcgcgcagc cccgccgccg ccagcctcgc
     9121 ccgcacgctc gcctgggtga gctcggccag caccggccac gcgccctccc ggtcgccgtt
     9181 ggcgaagcgc tcgagcgcct ccagcttttc gggcgtcgcc tgggccacga ggttttcggc
     9241 ctcggcgcgg taggcgtccc gctggacggc ccattcggag tcgcgcctgg acaggctctc
     9301 ggtgaggcgg gccaggtcgc gtcgggcctc gccgagggtc gcctccacct gcagccggcg
     9361 ctcacgctcc ttgcggctcg cgctttcggc gccgctgatg cgcagcgcga ggccgtggag
     9421 ctgggccgcg agggtcctga cgcgggcttc gtagcggccg atggtcgcaa gctggcggac
     9481 gtcggcgagc ctgcgctcgc ggatcaccgc ggcatcctgc tgggcgagga gcgcggcgac
     9541 caagggcgcg acccggcgcg gatcgttcgg cgcctgcgct cgagacgggg cggcccaccc
     9601 cgcgaccagg gcggctccgg ctatcccgaa catggacgca cgtcgaaggc ccatggcgcc
     9661 cccctcccgc tgcgcagcct agccggga
//
LOCUS       DASVQZ010000404         9755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_280351, whole genome shotgun sequence.
ACCESSION   DASVQZ010000404 DASVQZ010000000
VERSION     DASVQZ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280351"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VF547_11430"
     CDS             <1..254
                     /locus_tag="VF547_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX8263475.1"
                     /translation="VPAGEGSKSWARLEADVETLAARVRRRGHRPLLAGKDPRALLRD
                     LAEVRNPIYAQAHLRVLSDSGPHERTVDRIVEAMAERIR"
     gene            251..1264
                     /gene="aroB"
                     /locus_tag="VF547_11435"
     CDS             251..1264
                     /gene="aroB"
                     /locus_tag="VF547_11435"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX8263476.1"
                     /translation="MIRIDVALGERSYCVTIGERLLDRAGEWLAPHARGGRIVAVSDA
                     NVWPAQGARLAASGLDVEPVLVPAGEGSKSWARLEALTDRLLELGVGRGDALLAFGGG
                     MVGDLAGFAASVLKRGCAWVQVPTTLLAQVDSSVGGKTAINAAAGKNLIGAFHQPAAV
                     LIDPTTLDTLPARELRAGYAEVVKYGLIADPAFFDWCESNAAALLAGEAEARLHAIAT
                     CVRAKAAIVAQDELETTGRRALLNFGHTLGHAIEAETEMLHGEAVAIGMAAAFRRSVE
                     LGLCPPADSERVIAHLESVGLPTTTDVPAERLAERMRHDKKGGARVLTRGIGKAFLGR
                     AEP"
     gene            complement(1407..3179)
                     /locus_tag="VF547_11440"
     CDS             complement(1407..3179)
                     /locus_tag="VF547_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8263477.1"
                     /translation="MSQGVHAPVSLLGDAVILLGFALAFVLLFRRLGLGATLGYLVAG
                     ALVGPHLLGLVGGGESILEIAEIGIVLLLFLVGLELNPTRLWRMRHDIFGFGLLQVVL
                     CGIAASALIWLATGSSIAAAFALGLPLGLSSTAQVLPMLQSAGRLRTPFGERAFSILL
                     FQDLSIVPLITIIAAMSRNPADVGGPPGWLLGLYTVGAVFGLVLAGRYLLRPLFRLIG
                     NLGEREMFVFAGLFTVIASAAVMELLGLSTALGAFIAGVMLADSPYRHELQADVEPFR
                     SILLGLFFLAVGMMLDLGAIADRPLFVLAMALALIAIKAAVIMGLAMAFRMAWRPALA
                     LGVLLSQGGEFGFVLFAQAQQAFLIAPEAASLFGAIVTLSMATTPFLMMATARLRSEP
                     VSKEVRSGPVADGSNAVIVGYGRFGQTVAQMLIAQGIAVTLVDTDIEMIDVAGSFGAK
                     VYYGDGTRIDLLRQAGADEAELILFCTDGDQIEPEFLEGVHESFPNASIFVRAYDRRA
                     LLKLRDAPIAGAVREVLDSAVRMARMAMEKVGVGVEQIDRTEELYRARDRERLKAQLE
                     AGDLRARSERMFVEPAKTAEEEPG"
     gene            complement(3176..3616)
                     /locus_tag="VF547_11445"
     CDS             complement(3176..3616)
                     /locus_tag="VF547_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX8263478.1"
                     /translation="MPTALYTRDILRLAASIPNLGRLERPHASVERRSPVCGSRVVVD
                     LVVDGEGRVAALRQEVKACALGQASAALLGAHAEGRTREELAQARDALAAYLGGTRDD
                     PGAWPGLAVFADARRFPARHASILLAFDAAAEAAALAGAESARR"
     gene            complement(3687..5072)
                     /gene="cysS"
                     /locus_tag="VF547_11450"
     CDS             complement(3687..5072)
                     /gene="cysS"
                     /locus_tag="VF547_11450"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX8263479.1"
                     /translation="MIRFYDTAAREKRDFAPGDPGRVTMYVCGPTVYNRAHIGNARPA
                     VVFDVLARLLRRIYGEESVVHAANVTDVEDKIMAAAAAENVPIEVITERYSRFYFEDL
                     GALGVRRPDVVPLATGHIAEMIAMIGRLIAAGHAYAAEGHVLFDVPSWPSYGSLSRRP
                     MDEMIAGARVEVAPYKRSPADFVLWKPSAGDQPGWDSPWGRGRPGWHIECSAMIEAHL
                     GETIDIHGGGLDLEFPHHENEIAQSSCAHGGAPLARYWLHNGMLWMAASKMSKSLGNV
                     VTVGELLDEGHRGETLRLALLSAQYRQPLEWSPQLVAQSKATLDRLYRTLLAPSAGRG
                     KGDPAAVVEALADDLNTPLALSRLAELARLGDVDSTREAGALLGLLGHDPVEWFQGAA
                     ASVPDRVDPRLREEAGTFLDPAEIQRRIDRRAEAKSSRDYAEADRIRADLAAQGVLLE
                     DGPQGTTWRRA"
     gene            complement(5069..5629)
                     /locus_tag="VF547_11455"
     CDS             complement(5069..5629)
                     /locus_tag="VF547_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX8263480.1"
                     /translation="MTALDIIVLLLVGWNLVRGAMKGFVGEVLSLAAWIVAIVFLRLL
                     HTPVTNALESAVGTRPGAAVLAFALVFGLVFLAGKLVARQVGGGVRRSVIGPIDRVLG
                     AGFGALKGLILATLLYLAANLVYDTIWGAQAERPAWMSESRTYPLLYASGRALVDFVE
                     WRRGAPAEPRYRQAPPAKEAEPANAS"
     gene            complement(5789..7156)
                     /gene="radA"
                     /locus_tag="VF547_11460"
     CDS             complement(5789..7156)
                     /gene="radA"
                     /locus_tag="VF547_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX8263481.1"
                     /translation="MAKPKKRYVCQACGSVQNRWMGQCPDCTEWNSLVEDAGAVVTPF
                     SARHNLRGGGRAIELVGLDREVALPERMSTGIAELDRAIGGGLVAGSATLIGGDPGIG
                     KSTLLLQAAAMLASAGRDVAYVSGEEATDQVRLRARRLGLGSAPVQLAAATSVRDVLT
                     TLGEGAPPDLLVIDSIQTMYSDLIEGAPGTVSQVRASAGELIRYAKETGSALVLVGHV
                     TKDGAIAGPRVLEHMVDTVLAFEGERSHQYRILRATKNRFGGTDEIGVFAMAEAGLEQ
                     VANPSALFLTHRGEAVSGAVVFPALEGTRPVLVEIQALTVRLASGATPRRAVVGWDSG
                     RLAMILAVLEARCGLSFSSAEVYLNVAGGYRLSDPAADLAVAAALVSALAERPVPAEA
                     VAFGEVALSGELRPVAHGPLRLKEAAKLGFERALVPASVKGEGGMALSGFRTLKDLVD
                     HLLGR"
     gene            complement(7205..8008)
                     /locus_tag="VF547_11465"
     CDS             complement(7205..8008)
                     /locus_tag="VF547_11465"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX8263482.1"
                     /translation="MPAFPPAPTLLRRAVPPRVPAERPELPAYALSAGCWAALLLLPP
                     AAAATAFCAGGGDRLAALSFTAGAALSAVEWPAALGHWMLMIGAMMLPMAGMALRHVA
                     FRSFRRRRARAMTGFLLGYGAIWLLAAPAYLAAGLAGLAVRLASGTILIPLGIALGFA
                     AAWQGTAAKRRALRRSHRTVPLPPSGWAADRACLAYGLAQGRFCLASCWALMLVPAAA
                     GHHPLPMLLVGLFALLERRSPGAEPLRRPADAWDSLVRLAPSLDPLRVR"
     gene            complement(8010..9443)
                     /locus_tag="VF547_11470"
     CDS             complement(8010..9443)
                     /locus_tag="VF547_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX8263483.1"
                     /translation="MASAPPRVRKDVYKLASTDRTLEWYGRAINEMKKRPLNDPTSWR
                     YQAAIHGYSRAEDPLKKSGEALPSAADQAKFWNQCQHGSWYFLPWHRGYLLYFERICL
                     DAIVKLGGPKDWALPYWNYSAPGATPNQKLIPPAFRPATVAGKPNPLFAPGRTGGSTG
                     NAGIDPAEVSLDCLRRRNFVGFSSGGSPGFGGPQTGFHHGSGPSGHCERTPHGDIHVA
                     VNGFMASFNTAGLDPLFWLHHCNLDRLWEVWRLRSTSTGDPADPSWRNFPFNIHDQNK
                     AVVVFRSASMVSTTANGYKYQDVSDPFPAGTSILAGVEEGSMEDSDGRPAKLAGATDG
                     AITLGRGKTSARVTIEAPDVNILASVDGGGPQRVFLNIENITGEGPPGNYKVFVNVPE
                     GGDPEDHPDNFVGTLPLFGLGEQSAEDGPNGGSGLTHVLEITDVVQRLQDEGSWNESD
                     LDVDFVPIREVPAGADVRIGRVSVYRG"
BASE COUNT         1499 a         3372 c         3605 g         1279 t
ORIGIN      
        1 tggtccccgc cggcgagggc agcaagagct gggcccggct cgaggcggac gtcgagaccc
       61 tcgcggcgcg ggtcaggcgg cgggggcacc ggccgctgct cgccggcaag gacccgcgcg
      121 ccctcctccg cgacctcgcc gaggtccgga accccatcta tgcccaggcc catcttcgag
      181 tgctgagcga ttccggcccg cacgagcgca ccgtcgaccg gatcgtcgag gcgatggcgg
      241 agcggatccg atgatccgga tcgacgtcgc gctcggcgag cgcagctatt gcgtgacgat
      301 cggcgagcgc ctgctcgacc gcgccggcga gtggctcgcg cctcatgccc gcggcggacg
      361 gatcgtggcg gtctccgacg ccaatgtctg gccggcccag ggcgcgcggc tcgcggcctc
      421 cgggctcgac gtcgagccgg tgctggtccc cgccggcgag ggcagcaaga gctgggcccg
      481 gctcgaggcg ctgacggacc ggctgctcga gctcggagtc gggcgcggcg acgccctgtt
      541 ggcgttcggc ggcggcatgg tcggcgacct cgccggcttc gcggcctcgg tgctgaagcg
      601 cggctgcgcc tgggtgcagg tccccaccac ccttctcgcc caggtcgact cgtcggtcgg
      661 cggcaagacc gcgatcaacg ccgcggccgg caagaacctc atcggcgcct tccaccagcc
      721 ggccgcggtt ctgatcgacc cgacgaccct cgacaccctg ccggcgcgcg agcttcgcgc
      781 cggctatgcc gaggtcgtca aatacgggct gatcgcggac ccggccttct tcgactggtg
      841 cgaatcgaac gccgcggcgc tgctcgccgg cgaggccgag gcgcgcctcc acgccatcgc
      901 gacctgcgtc cgggccaagg ccgcgatcgt cgcacaggac gagctcgaaa cgaccgggcg
      961 ccgggccctg ctcaacttcg gccatacgct cggccacgcg atcgaggcgg agacggaaat
     1021 gctccacggc gaggcggtgg cgatcggcat ggcggcggcc ttccggcggt cggtcgagct
     1081 cggcctgtgc ccgcccgccg actccgagcg cgtgatcgcc catctcgaat cggtcgggct
     1141 gccgaccacg acggacgtcc cggccgagcg cctggccgag cggatgcgac acgacaagaa
     1201 gggaggcgcc cgggtgctga ctcgggggat cggaaaggcg tttctggggc gggcggagcc
     1261 gtagacgcct caagtcctcc ccgcgatttc gcggggaggg ggaccaggcg aagcctggtg
     1321 gaggggttct caccgactcc gcacaacccc tccaccgcgc tccgcgcggt ccccttcccc
     1381 ggaaatccgg gggagggatt tggcggctag cccggctcct cctcggccgt cttcgccggc
     1441 tcgacgaaca tccgctcgct cctcgcgcga aggtcgccgg cttcgagctg ggccttcagc
     1501 cgctcgcggt cgcgggctcg gtagagctcc tcggtgcggt cgatctgctc cactccgacg
     1561 ccgaccttct ccatcgccat ccgggccatc ctcaccgccg aatcgagcac ctcgcggacg
     1621 gcgcccgcga tcggcgcgtc gcgcagcttg agcaaggccc ggcgatcata ggcccggacg
     1681 aagatcgaag cgttggggaa ggactcgtgg accccttcga ggaactccgg ctcgatctgg
     1741 tcgccgtcgg tgcagaagag gatcagctcg gcctcgtccg cgccggcctg gcggagcagg
     1801 tcgatccggg tgccgtcgcc ataatagacc ttggcgccga agcttccggc gacgtcgatc
     1861 atctcgatgt cggtgtcgac gagggtgacc gcgatgccct gcgcgatcag catctgggcc
     1921 acggtctggc cgaagcggcc gtagccgacg atgaccgcgt tggagccgtc cgcgaccggg
     1981 ccgctccgca cctccttgct gaccggctcg gagcgcagcc gcgccgtcgc catcatcagg
     2041 aagggcgtgg tcgccatcga caaagtgacg atcgctccga acaggctcgc cgcctcgggc
     2101 gcgatcagga aggcctgctg cgcctgcgcg aacagcacga agccgaactc gccgccctgg
     2161 ctgagcagca ctccgagcgc cagcgccggc cgccaggcca tcctgaacgc catggcgagg
     2221 cccatgatca ccgccgcctt gatcgcgatc agggcgagcg ccatggcgag cacgaacagc
     2281 ggccggtccg cgatcgcgcc caagtcgagc atcatcccga cggcgaggaa gaacaggccg
     2341 agcaggatcg agcggaacgg ctcgacgtcg gcctggagct cgtgccggta gggcgaatcg
     2401 gcgagcatca ctccggcgat gaaggcgccc aaagcggtgg agaggccgag cagctccatc
     2461 accgcggcgc tggcgatgac ggtgaacagc ccggcgaaga cgaacatctc gcgctcgccc
     2521 agattgccga tcaggcggaa gagcggacga agcagatagc ggccggcgag gaccaggccg
     2581 aagacggcgc cgaccgtgta gaggccgagc agccagcccg gcggtccgcc gacgtcggcg
     2641 gggttgcggg acatggccgc gatgatcgtg atcaggggga cgatcgagag gtcctggaag
     2701 agcaggatcg agaaggccct ttccccgaac ggcgtgcgca ggcgccccgc cgactggagc
     2761 atcggcagca cctgggcggt cgaggagagg ccgagcggaa ggccgagggc gaaggcggcc
     2821 gcgatcgacg agccggtggc gagccagatc agggccgagg cggcgatccc gcacaggacg
     2881 acctggagca ggccgaagcc gaatatgtcg tgccgcatcc gccacagccg ggtcggattg
     2941 agctcgagcc cgaccaggaa gagcagcagg acgatgccga tctcggcgat ctcgaggatc
     3001 gattcgccgc cgccgaccag cccgagcagg tggggaccga ccagcgctcc ggcgacgaga
     3061 tagcccaggg tcgctccgag cccgaggcgg cggaagagga gcacgaacgc cagggcgaac
     3121 ccgagcagga tgacggcgtc gccgagcagc gacaccggcg cgtgcacgcc ttggctcacc
     3181 ttcgcgcgct ttccgcgccg gcgagggcgg ccgcctcggc ggcggcgtcg aaggcgagca
     3241 ggatcgaggc gtggcgggcg ggaaagcggc gggcgtcggc gaagacggcg aggccgggcc
     3301 aggcgccggg atcgtcgcgg gtgccgccga gataggcggc gagcgcgtcg cgggcctgag
     3361 cgagctcctc ccgggtccgg ccttccgcat gggcgcccag cagcgcggcc gaggcctggc
     3421 cgagggcgca ggccttcacc tcctggcgaa gcgcggccac cctgccctcg ccgtcgacga
     3481 cgaggtcgac gacgacccgg ctgccgcaga ccggcgagcg cctctcgacg ctcgcatggg
     3541 gccgttcgag ccggccgaga ttggggatcg aggcggcgag gcggaggatg tcgcgagtgt
     3601 agagggcggt cggcacgggg cgcctataga gggagcttgg cctcggcgcc acctcgcctc
     3661 cttcggcgaa gccgcggggc ggcagatcag gcccggcgcc aggtcgtgcc ctggggcccg
     3721 tcctcgagca gcacgccctg ggcggcgagg tcggcgcgga tccggtcggc ctcggcatag
     3781 tcgcgggagg acttggcctc ggcgcggcgg tcgatccggc gctggatctc ggccgggtcg
     3841 aggaaggtcc ccgcttcctc gcgaaggcgg gggtcgaccc ggtccggcac ggaggcggcg
     3901 gccccctgga accactcgac cggatcgtgg ccgagcaggc cgagcagggc gccggcctcc
     3961 cgggtcgagt cgacgtcgcc gagccgggcc agctcggcca ggcgggacag agcgagcggc
     4021 gtgttgagat cgtccgccag cgcctcgacg acggcggccg gatcgccctt ccctcgtccg
     4081 gccgagggcg caagcaaggt ccggtagagc cggtcgaggg tcgccttgga ctgggcgacg
     4141 agctgaggcg accattcgag cggctggcga tactgggccg agagcagggc gagccgaagc
     4201 gtctcgccgc gatggccttc gtcgagcagc tcgccgacgg tgacgacgtt gcccagcgac
     4261 ttcgacatct tgctcgccgc catccagagc atgccgttgt gcagccagta gcgcgcgagc
     4321 ggggcgccgc catgggcgca gctcgactgg gcgatctcgt tctcgtggtg cggaaactcg
     4381 aggtcgaggc cgccgccgtg gatgtcgatc gtctcgccca gatgggcctc gatcatcgcc
     4441 gagcattcga tgtgccagcc cgggcggccc cggccccagg gactgtccca gccgggctgg
     4501 tcgccggcgc tcggcttcca caggacgaag tcggcggggg agcgcttgta gggagccacc
     4561 tcgacccgcg cgccggcgat catctcgtcc atcggccgcc tcgacaggct gccgtaggag
     4621 ggccagctgg gcacgtcgaa cagcacatgg ccctcggcgg cataggcgtg accggcggcg
     4681 atcagccgac cgatcatcgc gatcatctcc gcgatgtggc cggtggcgag cggaacgacg
     4741 tccggccgcc gcacgcccag cgcccccaga tcctcgaaat agaagcggct gtagcgctcg
     4801 gtgatgacct cgatcggcac gttctccgcc gccgcggccg ccatgatctt gtcttcgacg
     4861 tcggtgacgt tggcggcgtg gacgacgctc tcctcgccat agattcggcg aagcagccgg
     4921 gcgaggacgt cgaacacgac cgccggccgg gcgttgccga tatgggcgcg gttgtagacg
     4981 gtggggccgc agacgtacat cgtcacccgg ccgggatcgc ccggcgcgaa gtcgcgcttc
     5041 tccctcgccg cggtgtcgta gaagcggatc acgaggcgtt ggccggctcg gcctccttgg
     5101 cgggcggcgc ctggcggtag cgcggctcgg ccggggcgcc tcggcgccat tcgacgaaat
     5161 cgaccagcgc ccgcccgctg gcgtagagca gcggataggt ccgcgattcg gacatccagg
     5221 ccggccgctc cgcctgggcg ccccagatcg tgtcgtagac gaggttggcg gcgagataga
     5281 gcagggtggc gaggatcagg cccttgaggg ctccgaagcc cgctccgagg acgcggtcga
     5341 tcgggccgat caccgagcgg cggacgccgc ccccgacctg gcgcgcgacc agcttgccgg
     5401 cgaggaagac gagtccgaag acgagggcga aggcgaggac ggcggcgccc gggcgggtgc
     5461 cgacggcgct ttcgagggcg ttggtgaccg gcgtgtggag cagcctcagg aagacgatcg
     5521 cgacgatcca ggcggcgagg ctgagcacct cgccgacgaa tcccttcatc gcccctcgaa
     5581 cgaggttcca gccgacgagc aggaggacga tgatgtcgag cgcggtcatg ctgcgagtgc
     5641 taacggaggt tcggtcggga gggaaggcga tccttcgata cgggccctcg ccaggctcag
     5701 tccctactca ggatgagcgg gggcgtcacg ctttccgctc atcctgagta gccgctgagc
     5761 gaagtcgaag cggcgtttcg aaggaccttc accgccccag cagatgatcc accaaatcct
     5821 tcagagtccg gaagccggag agcgccatgc ccccctcccc cttcaccgag gccggcacca
     5881 gcgcccgctc gaagccgagc ttggcggctt ccttgagccg caggggcccg tgcgcgaccg
     5941 ggcggagctc gccggacagg gcgacctcgc cgaaggcgac cgcctcggcc ggaaccggtc
     6001 gctccgccaa ggccgacacc agggccgcgg cgaccgcgag gtcggcggcc gggtcgctca
     6061 gccgatagcc gccggcgacg ttgagataga cctccgccga ggagaagctg aggccgcagc
     6121 gcgcctcgag cacggcgagg atcatggcga gccggcccga atcccacccg accaccgccc
     6181 ggcgcggggt ggcgccgctg gcgaggcgga cggtcagcgc ctggatctcg accagcaccg
     6241 ggcgggtccc ctcgagcgct gggaagacca cggcccctga taccgcctcg ccgcgatggg
     6301 tgaggaagag ggcggaggga ttggccacct gctcgaggcc ggcctcggcc atggcgaaga
     6361 ctccgatctc gtcggtgccg ccgaagcggt tcttggtcgc gcgcaggatc cggtattggt
     6421 ggcttcgctc gccctcgaag gcaaggacgg tgtccaccat atgctcgagc acacgggggc
     6481 ccgcaatcgc cccgtccttg gtgacgtggc cgaccaggac cagggccgag ccggtttcct
     6541 tggcgtagcg gatcagctcc ccggccgagg cccggacctg gctgacggtg ccgggggcgc
     6601 cctcgatcag gtccgaatac atggtctgga tcgagtcgat gaccagcaga tcgggcggag
     6661 cgccttcgcc cagggtggtc agcacgtcgc gaaccgaggt cgccgcggcg agctggacgg
     6721 gcgccgagcc gaggccgagc cgccgggcgc ggagccggac ctggtcggtg gcctcctccc
     6781 cggaaacgta ggcgacgtcg cgcccggcgc tcgccagcat cgccgccgcc tgcagcagca
     6841 gggtcgactt gccgatcccg ggatcgccgc cgatcagggt ggcagagccg gcgaccagcc
     6901 cgccgccgat cgcccggtcg agctcggcga tgccggtcga catccgctcg ggcagggcga
     6961 cctcgcggtc gaggccgaca agctcgatcg cccggccgcc gccgcgcaga ttgtggcgcg
     7021 cggaaaaggg cgtgacgacc gctccggcgt cctcgaccag gctgttccat tcggtgcagt
     7081 cggggcactg gcccatccac cggttctgga cggagccgca ggcctggcag acgtagcgct
     7141 tcttgggttt ggccatcgcc cctggactag cgggagggcg cccggccagc cagagctttc
     7201 ggcgctatcg cacgcgcagc gggtcgagcg atggcgccag ccgaacgagc gaatcccagg
     7261 cgtcggcggg gcggcgcagc ggctcggcgc cgggggagcg ccgctcgagc agcgcgaaca
     7321 ggccgaccag cagcatcggc agcggatgat ggccggccgc ggccgggacg agcatcagtg
     7381 cccagcagga cgccaggcag aagcgcccct gggcgaggcc gtaggcgagg caggcgcggt
     7441 cggccgccca gccggagggc ggcagcggca cggtccggtg ggagcggcga agcgcgcgcc
     7501 gcttggccgc cgtcccctgc caggcggcgg cgaaccccag cgcgatgccg agcgggatca
     7561 ggatcgtgcc cgaggcgagc cggacggcga gcccggccag gccggcggcg agataggccg
     7621 gcgccgcgag aagccagatc gccccgtagc ccagcaggaa gccggtcatc gccctcgccc
     7681 gccggcggcg gaagctgcgg aaggcgacat ggcgaagcgc catccccgcc atcggcagca
     7741 tcatcgcccc gatcatcagc atccagtgcc ccagcgccgc cggccattcg acggcggaga
     7801 gcgcggcgcc ggcggtgaag gacaaagccg ccagccggtc cccgccgccg gcgcaaaagg
     7861 cggtggccgc cgctgccggg ggcaggagca gcagcgccgc ccagcagccg gcgctcagcg
     7921 cataggccgg cagctccggc cgctccgccg gaacgcgcgg cgggacggcg cggcggagca
     7981 gggtcggcgc gggggggaag gccggcacgt cagccgcgat agacgctgac ccgcccgatc
     8041 ctgacgtcgg cccccgccgg gacctcgcgg atcgggacga aatcgacgtc gaggtcggat
     8101 tcgttccagc tcccctcgtc ctgcagccgc tggacgacgt cggtgatctc gagcacatgg
     8161 gtcagcccgc tgcccccgtt ggggccgtcc tccgcgcttt gctcgccgag cccgaacaaa
     8221 ggcagtgtgc cgacgaaatt gtcggggtga tcctccggat ccccgccttc cgggacgttg
     8281 acgaagacct tgtaattgcc cggcgggcct tcgccggtga tgttctcgat gttgaggaac
     8341 acgcgttgcg gcccgccgcc gtcgacggac gcgaggatgt tcacgtcggg cgcctcgatc
     8401 gtcacccggg cgctggtctt gccgcggccg agcgtgatgg cgccgtcggt cgcgccggca
     8461 agcttcgccg gacgcccgtc cgaatcttcc atggaaccct cctcgactcc ggccaggatg
     8521 ctggtgcccg cggggaacgg gtccgagacg tcctgatatt tgtagccgtt ggcggtcgtg
     8581 ctcaccatgc tcgccgaccg gaacacgacc accgccttgt tctggtcgtg gatgttgaag
     8641 gggaagttgc gccagctcgg atcggccggg tcgccggtgc tcgtcgagcg cagccgccag
     8701 acctcccaga gccggtcgag gttgcagtgg tgcagccaga acagcgggtc gaggccggcc
     8761 gtgttgaacg aggccatgaa gccgttgacg gcgacgtgga tgtcgccgtg cggggtccgc
     8821 tcgcaatgtc cggacgggcc gctgccgtga tggaagccgg tctgcgggcc gccgaagccg
     8881 gggctgccgc cgctcgagaa gccgacgaag ttgcggcgcc gaaggcagtc caagctgacc
     8941 tccgccggat cgatgccggc gttgccggtg ctgccgccgg tgcggccggg cgcgaacagc
     9001 ggattgggct tgccggcgac ggtggcggga cggaaggccg gcgggatcag cttctggttc
     9061 ggcgtcgcgc cgggcgcgct gtaattccaa tagggcagcg cccagtcctt cggcccgccc
     9121 agcttgacga tcgcgtcgag gcagatgcgc tcgaaataga ggaggtagcc gcgatgccag
     9181 ggcaggaagt accagctgcc gtgctggcac tggttccaga acttggcctg gtcggcggcg
     9241 ctcggaagcg cctcacccga tttcttcagc ggatcctcgg cgcggctgta gccgtggatc
     9301 gcggcctggt agcgccagct cgtcgggtcg ttgagcggcc gcttcttcat ctcgttgatg
     9361 gcccggccat accattcgag cgtccggtcg gtcgaagcca gcttgtagac gtccttgcgg
     9421 acccgcggcg gcgcgctagc catattcgtc cccttcggtt gcgggcgcag cgcacccttc
     9481 cctacgcaac aggattccag actaaccgat tcccggatgc cgggcgcggg gaatctcagc
     9541 cgtttcggaa aacaggggcg agccgctgcg gatggggaaa aatccttggc gccgggggga
     9601 acagccgcgc cctccccgca ttcacagact gccagcgatg gagagtgcct tgtttatcca
     9661 gatccattcc gacaacaaga tcgacagcga cagcagccgc gacggccggc tcgaggagca
     9721 gatccgacag cgcctcgccc gcttcgagga ccgga
//
LOCUS       DASVQZ010000405         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_563797, whole genome shotgun sequence.
ACCESSION   DASVQZ010000405 DASVQZ010000000
VERSION     DASVQZ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563797"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..2395
                     /locus_tag="VF547_11475"
     CDS             263..2395
                     /locus_tag="VF547_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8263484.1"
                     /translation="MSRAGILLGAVAAGALAMASLASAQPDAPKTPPADEPIVPDSQF
                     EKELPSLDPELGKPLEPLEDFTGPPPAATPEGELIPDAPVDDSELTRPLTPISEFDVR
                     TPPPAPEGKEDEGKVEPVRYTLVVEGLDEVQLEDRFRELSALEDADGEATNGAQVSAR
                     AKEDELLAVRLLRSEGYYDATALSSIEQLPDQPGQMRVVVSAVPGPRYLFGNIAITGP
                     QTEPPELAREALKLKTEDPIVAANVEAAEANVRLRLPQQGYPFAEVGLRDILLDPETR
                     HGDYTLPIDPGARSRFAGFTTEGDLAFGAKHVGVLARFKRGDLYDSRKVDDLREAMIA
                     TNLFTTVAAETVPTGEKAEDGTEYVNILVRQDAGPARSLAGTAGYSTGEGFRAEATWE
                     HRNIFPPEGSLRLGAILGTQEQVVRAGFRRNNWKKRDRSIFIQAEAGRRDLPAFKGYT
                     ARLQAQAARESTPIWQKVWTYALGAELIATNEAQTGFPPLSISDAFFLAGVSAQVGYD
                     RSNSLLDPTKGFRLKAFVNPETSLREPGQPYIRNILDGSVYYPVQDNIVIAGRARVGS
                     IYGAELAELAPSRRLYAGGGGSVRGFGFQELGPKIEVANPDFDPTDPEEEDDPTMLIP
                     TGGRSMVEFALEGRYRFGNYGVVGFVDAGQVYESQSPRLSDMRFGVGVGGRLYTNFGP
                     MRADVAMPIGRRKGESRFAVYVSIGQAF"
BASE COUNT          396 a          901 c          834 g          362 t
ORIGIN      
        1 gccgccgggg acggggacgg cctcggcgct tcggcaggcg cagaaccggc tgatgtcggc
       61 gcccgacacc tcccatccct attattgggc ggggttcgcg atcgtcggcg acggagccgc
      121 tccggtgctt ccgaaaagct gacccaaagc gtggccgcca agacacgtcg gctacccctt
      181 acgcctccgg aacaaatgca agcttttcca gttgaacgtt tttcggcgta agggggagct
      241 tcgagctgga gcgaagggcg atttgagcag agccggaatc ctgttgggcg cggtggccgc
      301 cggagcgctc gccatggcct cgctcgcctc ggcgcaaccg gacgccccga agacgccgcc
      361 cgccgacgag ccgatcgtcc ccgacagcca gttcgagaag gagctgccgt cgctcgatcc
      421 ggagcttggc aagccgctcg agccgctcga ggatttcacc ggccccccgc ccgcggcgac
      481 gcccgagggg gagctgatcc ccgacgcccc ggtcgacgac tccgagctca cccgtccgct
      541 gacgccgatt tccgaattcg acgtgaggac cccgccgccc gccccggagg ggaaggagga
      601 cgaaggcaag gtcgagcctg tccgctacac cctggtcgtc gaggggctgg acgaggtcca
      661 gctcgaggac cgcttccgcg agctttccgc gctcgaggac gccgacggcg aggcgaccaa
      721 cggcgcccag gtctcggccc gtgcgaagga ggacgagctg ctcgccgtcc gcctgctccg
      781 ctcggagggt tattacgacg ccaccgctct gtcctcgatc gagcagctgc ccgaccagcc
      841 ggggcagatg cgggtggtgg tcagcgccgt ccccgggccc cgctacctgt tcggcaacat
      901 cgcgatcacc ggcccccaga ccgagcctcc cgagctcgcc cgcgaggcgc tcaagctcaa
      961 gacggaggac ccgatcgtcg ccgccaacgt cgaggcggcc gaggccaatg tccggctccg
     1021 cctgccccag cagggctatc cgttcgccga ggtcggcctg cgcgacatcc tgctcgaccc
     1081 cgagacccgc cacggcgatt acaccctgcc gatcgatccc ggcgcccgct cgcgcttcgc
     1141 cggcttcacc accgagggcg acctcgcctt cggcgccaag catgtcggcg tcctcgcccg
     1201 cttcaagcgc ggcgacctct acgacagccg caaggtcgac gacctcaggg aggcgatgat
     1261 cgccaccaat ttgttcacca ccgtcgccgc cgagaccgtg ccgaccggcg agaaggccga
     1321 ggacggcacc gaatatgtga acatcctcgt ccgccaggac gccgggccgg cgcggtcgct
     1381 ggccgggacc gcgggctact cgaccggcga gggctttcga gccgaggcga cctgggagca
     1441 ccgcaacatc ttcccgcccg aggggagcct gcggctcggc gcgatcctcg gcacccagga
     1501 gcaggtggtg cgcgccgggt tccgccgcaa caactggaag aagcgcgacc gaagcatctt
     1561 catccaggcc gaggccgggc gccgcgacct gcccgccttc aagggctata ccgcccggct
     1621 ccaggcccag gccgcccgcg agtcgacccc gatctggcaa aaggtctgga cctatgcgct
     1681 cggcgcggag ctgatcgcca cgaacgaggc ccagacgggt ttcccgccgc tgtcgatcag
     1741 cgacgccttc ttcctcgccg gcgtcagcgc gcaggtcggc tacgaccgct cgaacagcct
     1801 cctcgatccg accaagggct tccgactcaa ggcgttcgtc aacccggaga cgtcgctgcg
     1861 cgagccgggc cagccctata tccgcaacat cctcgacggc agcgtctatt atccggtgca
     1921 ggacaatatc gtcatcgccg gccgggcgcg ggtgggctcg atctacggcg ccgagctggc
     1981 cgagctggcg ccgtcgcggc ggctctatgc gggcggcggc ggatcggtcc gaggcttcgg
     2041 attccaggag ctgggcccca agatcgaagt ggcgaacccc gatttcgatc cgaccgatcc
     2101 cgaggaggag gacgatccga ccatgctgat cccgaccggc gggcggagca tggtcgagtt
     2161 cgcgctcgag ggccgctacc gcttcggcaa ttacggcgtc gtcggcttcg tcgacgccgg
     2221 gcaggtctat gaaagccagt cgccgcgcct ttccgatatg cgcttcggag tgggcgtcgg
     2281 cggccggctc tacacgaatt tcggtccgat gagggccgac gtcgcgatgc cgatcggccg
     2341 ccgcaagggc gaatcgcgct tcgcggtcta tgtctcgatc gggcaggcct tctgatggcg
     2401 agcgttgcgg agccgatccc gcgcggccgc cagcgcgccc gcacggcggc caaatggacc
     2461 ggcatcggac tgatcgggat tgtcgtcctc gtc
//
LOCUS       DASVQZ010000406         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_374467, whole genome shotgun sequence.
ACCESSION   DASVQZ010000406 DASVQZ010000000
VERSION     DASVQZ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374467"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VF547_11480"
     CDS             complement(<1..344)
                     /locus_tag="VF547_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8263485.1"
                     /translation="MLRVLTLSTLYPDDGRPSFGPFVERQTLALAARPGVGVEVIAPV
                     GLPIWPLRLHPHYRARSALPAKEQRRGLAVHRPRFRTWPGLEAAGAAGSLAAAVLPLA
                     RALPCDVLDAEF"
     gene            482..1279
                     /locus_tag="VF547_11485"
     CDS             482..1279
                     /locus_tag="VF547_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX8263486.1"
                     /translation="MLRFPARSVAAVMMVSLVLPLAACAGRGKRTKADTEYVARDVNT
                     LYNLARKRLDQRDYPGAGAVFDEVERQHPYSVWARRAQLMSAFSYYAARDYTKSVESA
                     RRFLSIHPGNRDAPYAYYLIALSYYEQIEDVTRDQSTTRQALDALGELVRRYPDTRYA
                     ADARLKIDLVRDHLAGKEMEIGRFYQRRGQWLASVMRFRQVVDQYQTTSHTPEALMRL
                     TESYLALGIPEEARKSASVLGANYPNTKWYERAYSLVQKHAPAAPAA"
     gene            1383..3044
                     /gene="recN"
                     /locus_tag="VF547_11490"
     CDS             1383..3044
                     /gene="recN"
                     /locus_tag="VF547_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX8263487.1"
                     /translation="MLTSLSIRDVVLIESLDLEFGPGLGVLTGETGAGKSILLDALGL
                     ALGTRADSGLVRQGQAQAVVTVSFDLPGDHPALDLLRDNGLEGEPGEPLVVRRIVRAD
                     GGSRALVNDQSASVALVRELGATLVEIHGQHDDRGLLNPRGHRALLDAFGRIDTAAAE
                     RSYLAWGEAEASLARLRQDLDAAARDREWLEHAVAELTRLAPEPGEEEALAGLRADMQ
                     KGARLAEDIAAIDQLLDGSDGGLSLLRQAARRLERIGGEHDRLGEALAALDRALAEAS
                     EAEERLAAAAEALAYDPARLDEAETRLFDIRALARKHRVEPDALAALAEELGARLAAL
                     DAGERGLAELERKVSDSRSAYEAEAARLTELRIAAAARLDSAVARELAPLKLDSARFR
                     TLVEPLAEPNWAARGRDRVEFEVSTNPGAPFAPLIRIASGGELARFILALKVALAERG
                     EARTMIFDEIDRGVGGAVASAVGERLHRLAERAQLLVVTHSPQVAARGHRHLLIEKSH
                     EGLVTRTSVQTLDEARRREEIARMLSGAQVTDEARAQAVRLLEAA"
BASE COUNT          457 a         1177 c         1051 g          400 t
ORIGIN      
        1 aagaactcgg cgtcgaggac gtcgcagggg agggcgcgcg ccagcggcag gaccgccgcc
       61 gccagcgagc cggccgcgcc ggcggcctcc aggcccggcc aggtccggaa gcgcggccgg
      121 tggacggcga ggccgcggcg ctgctccttc gccggaagcg cggacctcgc gcgatagtgc
      181 ggatggaggc gcaagggcca gatcggcagc ccgaccggag cgatcacctc gaccccgacc
      241 ccgggacggg ccgccagagc cagggtctgc cgctcgacga acggcccgaa ggacggccgc
      301 ccgtcgtccg ggtagagcgt cgacagggtc agaacgcgaa gcatcgccgc tccctacagc
      361 ccacacatta aaccgcggtt catcggcgac ggcggagtga cggtcactag tgacagtcgc
      421 tagtgactgt cactcccagt ccgcgcttgg cctcgtccca acccttcgct aagaagcccc
      481 catgctgcgt ttccctgccc gctctgtcgc cgccgtcatg atggtgagcc tggtccttcc
      541 gctcgccgcc tgtgccggcc ggggcaagag gaccaaggcc gacaccgaat atgtcgcccg
      601 cgacgtcaac acgctctaca acctcgccag aaagcggctc gaccagcgcg actatcccgg
      661 cgccggcgcc gtgttcgacg aggtcgagcg ccagcatccc tattccgtct gggcccgccg
      721 cgcccagctg atgagcgcgt tcagctatta cgccgccagg gattacacca agtccgtcga
      781 atccgcacgg cgcttcctgt cgatccaccc cggcaaccgc gacgcgccct acgcctatta
      841 cctgatcgcg ctcagctatt acgagcagat cgaggacgtc acccgcgacc agagcacgac
      901 ccggcaggcg ctcgacgcgc tcggcgagct ggttcgccgc tatccggaca cgcgctacgc
      961 cgcggacgcc cgcctcaaga tcgacctcgt gcgcgaccac ctcgccggca aggagatgga
     1021 gatcggccga ttctaccagc gccgcggcca gtggctcgcg tcggtgatgc gcttccgcca
     1081 agtcgtcgac caatatcaga ccaccagcca cacgcccgaa gcgctgatgc ggctcaccga
     1141 atcctatctc gccctcggca tccccgagga ggcccgcaag tccgcctcgg tgctcggcgc
     1201 caattatccc aacaccaaat ggtacgagcg ggcctattcg ctggttcaga agcacgcgcc
     1261 ggcggcgccg gccgcgtgac ggtgaaatgc cgtcacccca gcgcaagctg gggtccatgc
     1321 ggcgtcggcg cgctaggccc gcatggatgc cagccttcgc tggcatgacg ggttaagggg
     1381 cgatgctcac cagcctctcc atccgcgacg tcgtcctgat cgagagcctc gacctcgaat
     1441 tcggcccggg cctgggcgtt ctcaccggcg agaccggcgc cggcaagtcg atcctgctcg
     1501 acgccctcgg cctggcgctc ggcacccgcg ccgacagcgg cctcgtccgc caggggcagg
     1561 cgcaggcggt ggtcaccgtc agcttcgacc tgccgggcga tcatcccgcg ctcgacctgc
     1621 ttcgcgacaa cgggctcgag ggcgagcccg gcgagcccct ggtcgtccgg cgcattgtcc
     1681 gcgccgacgg cggcagccgc gccctcgtca acgaccagtc ggcgtcggtc gccctggtcc
     1741 gcgagctcgg cgcgacgctg gtcgagatcc acggccagca cgacgatcgc ggccttctca
     1801 acccgcgcgg ccaccgcgcc ctgctcgacg ccttcggacg gatcgacacc gccgcggccg
     1861 agcgatccta tctcgcctgg ggcgaggccg aggcgtcgct cgcccggctg cgccaggatc
     1921 tcgacgccgc cgcccgcgac cgcgaatggc tcgaacatgc cgtcgccgag ctgacccggc
     1981 tcgcgcccga gcccggtgag gaggaggctc tcgccggcct ccgcgccgac atgcagaagg
     2041 gcgcccgcct cgccgaggac atcgccgcga tcgaccagct gctcgacgga tcggacggcg
     2101 gcctctccct gctccggcag gcggcgcgcc ggctcgagcg gatcgggggc gagcacgatc
     2161 ggctcggcga ggcgctcgcc gcgctcgacc gggcgctggc ggaggcgtcg gaggccgagg
     2221 agcggctcgc cgccgccgcc gaggcgctgg cctatgatcc cgcccggctc gacgaggccg
     2281 agacccgcct gttcgacatc cgggctctcg cccgcaagca ccgggtcgag ccggacgcgc
     2341 tcgccgcttt ggccgaggag ctgggcgctc ggctcgcggc gctggacgcc ggcgagcggg
     2401 gccttgccga gctcgagcgc aaggtgagcg acagccgctc cgcctacgag gccgaagccg
     2461 cccgcctcac cgagctgcgc atcgccgccg ccgcccggct cgactcggcc gtcgcccgcg
     2521 agctcgcgcc gctcaagctc gactcggcgc gcttccgcac cctcgtcgag ccgctcgccg
     2581 agccgaactg ggccgcgcgc ggcagggacc gggtcgagtt cgaggtctcg accaaccccg
     2641 gcgctccgtt cgcgcctctc attcggatcg cgagcggcgg cgagctcgcc cgcttcatcc
     2701 tcgccctcaa ggtggccctg gccgagcgcg gcgaggctcg cacgatgatc ttcgacgaga
     2761 tcgatcgcgg agtcggcggc gccgtcgcca gcgccgtcgg cgagcggctc caccggctgg
     2821 cagagcgcgc gcagctgctc gtcgtcaccc acagcccgca ggtcgcggcc cgcggccacc
     2881 gccacctcct catcgagaag agccacgagg ggctggtgac ccgcaccagc gtgcagacgc
     2941 tggacgaggc ccgccgccgc gaggagatcg cccgcatgct gtccggggcg caggtgaccg
     3001 acgaggcgag ggcgcaggcg gtaagattgc tggaggcggc gtgagggttt gcgggagaag
     3061 ggtcctcccc gcgatttcgc gggga
//
LOCUS       DASVQZ010000407         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_949675, whole genome shotgun sequence.
ACCESSION   DASVQZ010000407 DASVQZ010000000
VERSION     DASVQZ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949675"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VF547_11495"
     CDS             <1..235
                     /locus_tag="VF547_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8263488.1"
                     /translation="GMLPTALAIGSDGAWNQPMAVTVIGGLILSTLLTLLIVPAGFSL
                     ADSFEQWLAQRLGRLLTYRGEEGGPKPAPQPAE"
     gene            305..1507
                     /locus_tag="VF547_11500"
     CDS             305..1507
                     /locus_tag="VF547_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX8263489.1"
                     /translation="MRIIATGMLVAMAAVFLAANAWDEAFPWLGFVKAFAEAAMVGGL
                     ADWFAVTALFRHPLGLPIPHTAIIPRNKDRIGEALAAFLRENFLIPAVVARRMRNVDV
                     AGAIGRFLANPPPEGRLRQGGSRLIADLLESLDQERLGGMVKTAVASRMRTLEVSPLL
                     GQTLEAAITEERHIPILDGIVTWAGRTLDANEDLIRKMVHERAGMIMRLSGLDEKLAD
                     KIVDGLRRLTIDMAIDPAHPLRAKAEEALAALAWDMRFDPEMQGKVEGWKNEIIANKA
                     VTDWLGGLWENSRAALLRAARDPEAAMAGRFGEALRQLGETLQQDQRLNKAINQFARR
                     MVVGTAATYGSGIVTLVSDTVRGWDARTVTGRLENAVGRDLQYIRINGTLVGGLVGLL
                     IHTVEVAA"
     gene            1570..>1974
                     /locus_tag="VF547_11505"
     CDS             1570..>1974
                     /locus_tag="VF547_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8263490.1"
                     /translation="MKLVRTSLTALAIAFAGPSLAQTAVPAPAAAAEPVTVLHAGTLM
                     AVPGEPPRREASVIVRGRRIVEIRSGYIDMPGARIVDLRDSTVMPGFIDMHVHLRGLD
                     DRLQARLQAPLRDYEDEAYTAQLNARRTLLAGF"
BASE COUNT          310 a          690 c          679 g          295 t
ORIGIN      
        1 cggcatgctg ccgacggcgc tggcgatcgg gagcgacggc gcgtggaacc agccgatggc
       61 ggtgaccgtg atcggcggcc tcatcctgtc gacgctcctc acccttctca tcgttccggc
      121 cggcttcagc ctcgccgaca gcttcgagca gtggctcgcg cagcgcctgg gccggctgct
      181 cacctaccgc ggcgaggagg gcggcccgaa gcctgccccg cagccggccg agtgaaccat
      241 cgccgcgcgc ggggattggg ataggatggt ccccgcggaa gccccccggc acggcatcag
      301 gcagatgcga atcatcgcca ccggcatgct ggtggcaatg gcggcggtgt tcctcgcggc
      361 caacgcctgg gacgaggcct ttccatggct cggcttcgtc aaggccttcg ccgaggcggc
      421 gatggtcggc ggactcgccg actggttcgc ggtcacggcc ctgttccgcc acccgctcgg
      481 cctgcccatc ccgcacacgg cgatcatccc gcgcaacaag gaccggatcg gggaagcgct
      541 cgccgccttc cttcgcgaga acttcctgat cccggcggtg gtggcgagac ggatgcgcaa
      601 tgtggacgtt gccggcgcca ttgggcgatt cctggcgaat cctccaccgg agggacgcct
      661 tcgtcagggc ggatcgaggc tgatcgccga cctgctcgaa tcgctcgatc aggagcggct
      721 cggcgggatg gtcaagaccg ccgtcgcctc gcggatgcgg accctcgaag tctcgcccct
      781 tctcggccag acgctcgagg ccgcgatcac cgaggaaagg cacatcccga tcctcgacgg
      841 gatcgtcacc tgggccggcc gcactctcga cgccaacgag gatctgatcc gaaagatggt
      901 gcacgagcgc gccgggatga tcatgcggct ctccggtctc gacgagaagc ttgccgacaa
      961 gatcgtcgac gggctccgcc gcctcaccat cgacatggcg atcgatccgg cccatccgct
     1021 tcgcgccaag gccgaggagg cgctcgccgc cctggcctgg gacatgcgct tcgatccgga
     1081 gatgcagggg aaggtcgagg gctggaagaa cgagatcatc gccaacaagg cggtgaccga
     1141 ctggctcggg ggcctgtggg agaacagtcg cgccgccctg ctgcgcgcgg cgcgcgaccc
     1201 cgaggcggcg atggccggcc ggttcggcga ggcgctgcgg caattgggcg agaccctcca
     1261 gcaggaccag aggctcaaca aggcgatcaa ccagttcgcc cgtcggatgg tcgttggcac
     1321 cgccgccacc tacggcagcg gcatcgtcac cctggtttcg gacacggtgc gggggtggga
     1381 tgcacgcacg gtgaccgggc ggctggagaa tgcggtcggc cgcgacctcc agtatatccg
     1441 gatcaacggg acgctggtgg gagggctggt cgggctcctc atccacacgg tcgaggtcgc
     1501 cgcctgatat cctcttgaag acggagcgct ttgccgcgca tgggggcatc gcaagacaag
     1561 gggatgatca tgaagctggt tcgcacctcg ctcaccgccc tggcgatcgc cttcgccggt
     1621 ccctcgctcg cccagaccgc ggtgcccgcc ccggccgcgg cggccgaacc ggtcaccgtc
     1681 ctccacgcgg ggacgctgat ggcggtcccc ggcgagccgc caaggcgcga agcgagcgtc
     1741 atcgtccgcg gccgccgcat cgtcgagata cggagcggct atatcgacat gccgggggcc
     1801 cggatcgtcg accttcgcga cagcacggtg atgcccggct tcatcgacat gcacgtccac
     1861 ctgcgcgggc tggacgaccg gctgcaggcg cgcctccagg cgccgctgcg cgactatgag
     1921 gacgaagctt atacggccca gctcaatgct cgccgcaccc tcctcgccgg cttc
//
LOCUS       DASVQZ010000408         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_949917, whole genome shotgun sequence.
ACCESSION   DASVQZ010000408 DASVQZ010000000
VERSION     DASVQZ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949917"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /gene="cutA"
                     /locus_tag="VF547_11510"
     CDS             complement(<1..285)
                     /gene="cutA"
                     /locus_tag="VF547_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX8263491.1"
                     /translation="MSAIVTVYATFPSEEEARRIARILVEERHAACANILGDCHSIYN
                     WQGAVEETAEIAALFKTRADLADALIARLAELHSYDVPAAVAWPVSNAWLP"
     gene            complement(282..1382)
                     /locus_tag="VF547_11515"
     CDS             complement(282..1382)
                     /locus_tag="VF547_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX8263492.1"
                     /translation="MATPAATLPAAPGARPRAIAVWLLAVAALVFLMIVVGGITRLTE
                     SGLSIVRWEPLSGAIPPIGEEAWAAEFAAYKSSPQYQLVNSGMSLDDFKTIYFWEYVH
                     RLLGRIIGLAFALPLLWFAWRRAVPSGYAWKLVALLALGGLQGAIGWWMVASGLVDRP
                     DVSHIRLAVHLLMALAIFGTTLWLALDLLRLAWEPGAGPARMPTSAIWMLSLLFLQFL
                     FGAYVAGLDAGYAYSSWPKMGDQWFPAEAPLLEPFLRNFADNPIVVQFVHRWLAFAVA
                     AAALVLARNAWREGHREAGGALLGAVTVQILLGIFTLLSGVELWIAASHQGMAVLLLA
                     AAIVAAHRLGERPAASARPHRPGPLQSEAASA"
     gene            1501..1869
                     /locus_tag="VF547_11520"
     CDS             1501..1869
                     /locus_tag="VF547_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEX8263493.1"
                     /translation="MLAKAVLSGSMDRMAIALSGLCLVHCLATSVILAVLASAGGLLV
                     SPVVHEIGLSLAILLGIVALGRGVVEHGFMMPSAVGGLGLGVMSGALTMPHSGLETVY
                     TIIGVAVLALGHDLNRRAVI"
     gene            2007..2474
                     /locus_tag="VF547_11525"
     CDS             2007..2474
                     /locus_tag="VF547_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX8263494.1"
                     /translation="MAHSHHHEAHEGASLMQAAQSSMEKSGEQWTQMRSAVFEALAAF
                     DRPASAYDIADKVSQSQGRRVAANSVYRILDLFVGANLARRVESANAYVANDHPDCLH
                     DCIFLICDTCGNTTHLDDDKLSGGVRAAASAAGFSPERPVIEVRGTCPDCKEG"
     gene            2557..3366
                     /locus_tag="VF547_11530"
     CDS             2557..3366
                     /locus_tag="VF547_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8263495.1"
                     /translation="MLVWVQENRDRLRSAGAVGLLHAVLGWLLLTGLGFSPAEVVPET
                     LKLLDIVEEPPPPPAKPAPPSVEKKTRKARPKDPEGSASPANLKNTPTEIMAPPPEIR
                     LPVPPPIPVAPAPGTGSAMAAGAATVPGPGTGAGGIGTGLGSGRFGNGTGGGGGGGRP
                     TPARQIAGSINNSDFPDEAYPNGFDGVAHLRFTVAPSGRVSDCAVTRSSGSRRLDNLT
                     CRLILQRFRYRPARNGEGIPISSVITGEHLWHAEPAPPDRWVEPDIEEPVD"
     gene            3463..>4119
                     /locus_tag="VF547_11535"
     CDS             3463..>4119
                     /locus_tag="VF547_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX8263496.1"
                     /translation="MTDRPNTPLLDTVGTPAELRRLEPGQLRQLADELRAETISAVSV
                     TGGHLGAGLGVVELTVALHYVFDTPEDRLIWDVGHQAYPHKILTGRRDRIRTLRQGGG
                     LSGFTRRSESEYDPFGAAHSSTSISAALGFAVANKLDDRPGRAIAVIGDGAMSAGMAY
                     EAMNNAQIAGNRLVVILNDNDMSIAPPVGSLRNALARLVSSGKYLAPRRLAQKLARKM
                     P"
BASE COUNT          647 a         1470 c         1413 g          589 t
ORIGIN      
        1 gggcagccaa gcgttcgaga cgggccacgc gaccgccgcc ggcacgtcgt agctgtgcag
       61 ctcggcgagg cgggcgatca gggcgtcggc caggtcagcg cgggtcttga acagcgcggc
      121 gatctcggct gtttcctcca cggcgccctg ccaattgtag atggaatggc agtcgccgag
      181 gatgttggcg caggcggcgt ggcgttcctc taccaggatg cgggcgatcc ggcgcgcctc
      241 ttcctccgaa gggaatgtgg cgtagaccgt gacgattgcg ctcatgccga tgcagcctcg
      301 ctttgcaggg gtccgggccg gtgcggccgg gccgaggccg ccggtcgttc gccgagccgg
      361 tgcgcagcca cgatcgcggc ggcgagaagc agcactgcca tcccctggtg cgaggccgcg
      421 atccagagct ccacgccgga aagcagggtg aagatcccga gcaggatctg aaccgtcacc
      481 gcgcccagga gcgcaccccc ggcctcacgg tgcccctccc gccaggcgtt gcgagcgagg
      541 accagagccg ccgccgcgac cgcgaaggcg agccagcggt ggacaaactg gaccacgatc
      601 ggattgtcgg cgaagttcct caggaacggc tcgagcagcg gggcctcggc ggggaaccac
      661 tggtcgccca tcttcggcca gctcgaataa gcgtaacccg cgtcgaggcc ggcgacatag
      721 gcgccgaaca ggaactggag gaagagcagg gagagcatcc agatcgccga ggtcggcatt
      781 cgcgccggcc cggcgccggg ctcccaggcg aggcggagca ggtcgagcgc aagccacagg
      841 gtcgtgccga agatcgcgag cgccatgagc aggtggacgg cgaggcggat gtggctgacg
      901 tccgggcggt cgacgaggcc ggaggcgacc atccaccagc cgatcgcgcc ctggagcccg
      961 cccagcgcga gcagggcgac gagcttccag gcatagccgc tcgggacggc ccgtctccag
     1021 gcgaaccaca gcagcggcag ggcgaaggcg aggccgatga tccggccgag gagccggtgg
     1081 acatattccc agaaatagat ggtcttgaag tcgtccaggc tcatgccgct gttgaccagc
     1141 tgatattgcg ggctgctctt gtaggccgcg aactcggccg cccacgcctc ctcgccgatc
     1201 ggcgggatcg ctcccgacag cggctcccag cgcacgatcg acaggcccga ttcggtcagc
     1261 cgggtgatgc cgccgacgac gatcatcagg aagacgaggg cggcgaccgc gagcagccag
     1321 acggcgatcg cccgcggtcg ggcgccgggc gcggcgggta gggtcgcggc gggtgtggcc
     1381 atggccggcg atatggcggc cggactgcgg aaagccaatg caactttttt cagaatgtta
     1441 tgttgtatct ttacggatgc gacattatag actggacgga cgaggagagc ggcaagtccg
     1501 atgttggcga aggcagtact gagcgggtcg atggaccgga tggcgatcgc gctgtccggg
     1561 ctgtgcctcg tccattgcct ggcgacctcg gtgatcctcg ccgtgctcgc ctcggccggg
     1621 gggctgctcg tctctcccgt cgtccacgag atcggtctgt ccttggcgat cctgctcggg
     1681 atcgtcgcgc tcggccgcgg ggtcgtcgag cacggcttca tgatgccctc ggccgtcgga
     1741 ggcctcggcc tgggtgtgat gtccggcgcg ctcaccatgc cccatagcgg cctcgagacc
     1801 gtctatacga tcatcggggt cgccgtcctc gccctcggcc acgatctcaa ccgccgagcc
     1861 gtgatctagg cgtcgtcgtc ccggcgcagg ccgggactcc agcgggcgaa aggtccacgc
     1921 gtccttcacc cgcggccgcc cggccggcgc tggcgcgacg gcggcgagcc cttgttccgc
     1981 cccggcaaaa gccttacctt gcgcccatgg cgcattccca ccaccacgag gcgcacgagg
     2041 gcgcttccct catgcaggcg gcgcagtcgt cgatggagaa atccggcgag cagtggaccc
     2101 agatgcgctc cgcggtcttc gaggcgctgg ccgccttcga ccggcccgct tccgcctacg
     2161 acatcgccga caaggtcagc cagtcccagg gccggcgggt cgccgccaac agtgtctacc
     2221 gaatcctcga cctgttcgtc ggcgccaacc tcgcccgccg ggtggagagc gccaacgcct
     2281 atgtcgccaa cgaccatccc gactgccttc acgattgcat cttcctgatc tgcgacacct
     2341 gcggcaacac cacccatctc gacgacgaca agctgtcggg cggggtgcgg gcggccgcca
     2401 gcgccgcggg cttctcgccc gagcgcccgg tgatcgaggt gcgcggcacc tgcccggact
     2461 gcaaggaagg ctgacccccg gggggctcgc aatacagcct gcagtcatct aaagccttgt
     2521 aatcgcctgt ctccatccct ggtcgggggc cggtcgatgt tggtgtgggt acaggagaac
     2581 agggatcgcc tgaggtcggc cggcgcggtc ggcctgcttc acgccgtcct cggctggctg
     2641 ctgctgaccg gactcggctt ctctccggcg gaggtcgttc cagagacctt gaagctgctc
     2701 gacatcgtcg aggagccgcc gccgcctccg gccaagccgg ccccgcccag cgtcgagaag
     2761 aagacgcgca aggccaggcc caaggatccc gaggggtccg cctcgccggc caatctcaag
     2821 aacaccccga ccgagatcat ggcgcctccg cccgagatcc gcctcccggt gccgccgccc
     2881 atcccggtcg ctccggcgcc gggcacggga agcgcgatgg cggcgggggc ggcgaccgtc
     2941 cccgggcccg gcaccggcgc cggcggcatc ggcaccggtc tcggcagcgg ccgcttcggc
     3001 aacggaaccg gcggcggcgg cggcggcggc cgtccgacgc cggcgcggca gatcgccggc
     3061 agcatcaaca attccgactt tcccgacgag gcatacccta atggcttcga cggcgtcgcc
     3121 cacctgcgct tcaccgtcgc tcccagcggc cgggtcagcg attgcgccgt gacccggtca
     3181 agcggcagcc gccggctgga caatcttacc tgccggctga tcctccagcg cttccgctat
     3241 cggccggcgc gcaatggcga agggatcccg atttcctcgg tgatcactgg cgagcacctc
     3301 tggcacgccg agcccgcgcc gcccgaccgc tgggtcgagc ccgacatcga ggagccggtc
     3361 gactgaccgc cccactgcac ctgccctgca catcatcccc attcgacagg cccgccgctt
     3421 tgggctagcg gggcggggcg acagggacca tatccgccag ccatgaccga ccgtccgaac
     3481 acccctctgc tcgacaccgt cggcaccccc gccgagctcc ggcggctcga gcccggccag
     3541 ctgcggcagc tcgccgacga gcttcgcgcc gagaccatct cggccgtctc ggtcaccggc
     3601 ggccatctcg gcgccggcct cggagtggtc gagctgacgg tggcgctcca ctacgtcttc
     3661 gacacgccgg aggaccgcct gatctgggac gtcggccacc aggcctatcc gcacaagatc
     3721 ctgaccggcc gccgcgaccg catccgcacg cttcggcagg gcggcggcct ctccggcttc
     3781 acccgccgaa gcgagagcga gtacgacccg ttcggagccg cgcacagctc gacctcgatc
     3841 tcggccgctc tcggcttcgc cgtcgccaac aagctggacg accggcccgg ccgggcgatc
     3901 gcggtgatcg gcgacggcgc gatgtcggcg ggcatggcct atgaggcgat gaacaacgcg
     3961 cagatcgccg gcaaccggct ggtcgtgatc ctcaacgaca acgacatgtc gatcgcgccg
     4021 ccggtcggaa gcctgcgcaa cgcgctcgcg cggctggtct cctcgggcaa gtatctcgcg
     4081 ccgcggcgcc ttgcgcagaa gctggcccgc aagatgccg
//
LOCUS       DASVQZ010000409         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_281009, whole genome shotgun sequence.
ACCESSION   DASVQZ010000409 DASVQZ010000000
VERSION     DASVQZ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281009"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF547_11540"
     CDS             complement(<1..327)
                     /locus_tag="VF547_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX8263497.1"
                     /translation="MTDAARTRSGLLMGLGAYTLWGVLPLYFKAMANVRATEIVAHRI
                     LWSLVFLGALATLLKRWPGIRAALADRRVALTLVLTACLIGTNWLVYIYAVMSGHVLE
                     GSLGYYL"
     gene            421..951
                     /locus_tag="VF547_11545"
     CDS             421..951
                     /locus_tag="VF547_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263498.1"
                     /translation="MHRLNLRAALAVGLLLGACQAKVDPEEADKGSAEPRASAEGKAE
                     EGKISVKAPGFDLKFTLPKGMVGEARAERDSKLLYPGAVLRGMAIAAGQDTGKGGDSE
                     VEIRFSTPDPLDEVVAWYREPARAEGFRLERAVKDGDGWMVTGVQKRDGHPFKLRLAP
                     RPGGGTDGRLMVRHRD"
     gene            962..1348
                     /locus_tag="VF547_11550"
     CDS             962..1348
                     /locus_tag="VF547_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX8263499.1"
                     /translation="MAALAEPDEVRLHLVAAARAGTALSYGELLGQLGYSFSRPKMRA
                     LCAILGAIDEAAERRGEPELAVLVVRQSDGLPGQGWWAAGGARSRGYEGPWDGPEARR
                     FIEEVQAESFAFWQSRSGTGTGEAPD"
     gene            1413..2057
                     /locus_tag="VF547_11555"
     CDS             1413..2057
                     /locus_tag="VF547_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263500.1"
                     /translation="MRDRAFSMLRLSALAGVLALSACNWLRSDDKAVAAANDADPEIA
                     NALNDQILVDGNLDNQSNRNGGRAPVAPGNARYPSADGGASGGAKGPDAVAALGKNCS
                     DAARFDYNMGWAKRLAPNFPVYPGGRVTEAAANNSSGCSTRVVTFTTDHDWQRILDWY
                     HTRAVRAGYSSEHQLRDGDHVLAGANAKDNTAFYLIVTPKPKGSEVALIANNGR"
     gene            2196..3290
                     /locus_tag="VF547_11560"
     CDS             2196..3290
                     /locus_tag="VF547_11560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8263501.1"
                     /translation="MSEVRQFTVAPDDDGIRLDRWFKRHLPETSFNTVSRWARTGQLR
                     LDGKRAAPGDRIEAGQAIRVPPAEPAAASASSARAKPERPRLSDDEIAFVNEIVIHRD
                     PHAIVVNKPPGLATQGGTGTRTHLDGLLDGLADEAGHRPKLVHRLDKDTSGVLLLART
                     ARAAAFFSKAFSGRTARKVYWAIVTGVPDIADGYIDLPLAKQPGTGGEKMHVEEGESG
                     LPTRTRYRMIERAGNRAAWLELQPLTGRTHQLRVHMAAIGHPILGDGKYGGPDAFLTG
                     TISRKLHLHARRLRIDHPDGGRLDVTAELPAHFAETMANLGFDPALGDLPLDEVKFSE
                     TAEGKRRAASAAAKSRRRERKGERRGRGRG"
     gene            3290..3892
                     /locus_tag="VF547_11565"
     CDS             3290..3892
                     /locus_tag="VF547_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX8263502.1"
                     /translation="MHPNRKFHIADREAMAGLVRSLGFGVLFVVTGEGPRAVHVPVLL
                     DGERLRFHVSRGNLVHSALAAGGTALFVATGPHAYISPEWYGLDDRVPTWNYVAVELE
                     GPVRTLTPDELVSLVDDLSAEQEARLAPKPAWTRAKMSPGRFEGLLKAITGFEMEVVE
                     WRGTAKIDQDKPEAVRSSVADALAARGEREMAETMRKAAP"
     gene            3889..4122
                     /locus_tag="VF547_11570"
     CDS             3889..4122
                     /locus_tag="VF547_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263503.1"
                     /translation="MTAQDDETLWRNRFIAINLVRIGGTIVVLIGLAIWHSDFLVEGG
                     SIAVGLPMALAGLVASFWGPKWLAARWRTPPGP"
BASE COUNT          652 a         1493 c         1487 g          554 t
ORIGIN      
        1 cagataatag ccgaggctgc cctcgagcac gtgcccgctc atcacggcgt agatgtagac
       61 cagccagttg gtgccgatca ggcaggcggt caggaccagg gtcaaggcga cccggcggtc
      121 ggcgagggcg gcgcggatgc ccggccagcg cttcagcaag gtcgcgagcg cgccgaggaa
      181 gaccagcgac cacaggatcc ggtgggcgac gatctcggtg gcccggacgt tcgccatcgc
      241 cttgaagtag agcggcagca ccccccagag cgtgtaagcc ccaaggccca tcagaaggcc
      301 ggatcgggtg cgggcggcgt cggtcaccgc ggcgcgggta gcgaatgggc ttggccgcga
      361 caagcatttg gcggccgccc gccgacgggc taggatggcg ccgcttgcag gggagaagga
      421 atgcaccgcc tcaatcttcg cgccgccctc gccgtcggcc tgctgctcgg cgcctgccag
      481 gcgaaggtgg atccggagga ggccgacaag ggctcggccg aaccccgggc ctcggccgag
      541 ggcaaggcgg aggagggcaa gatctcggtc aaagcgcccg gattcgatct caagttcacc
      601 cttcccaagg gcatggtcgg cgaggcgcgc gccgagcggg acagcaagct gctctatccc
      661 ggcgcggtgc ttcggggcat ggcgatcgcc gccggccagg acaccggcaa ggggggcgac
      721 agcgaggtcg agatccgctt ctcgaccccg gacccgctcg acgaggtggt cgcctggtat
      781 cgcgagccgg cccgcgccga gggcttccgg ctggagcgcg cggtcaagga cggcgacggc
      841 tggatggtga ccggcgtgca gaagcgcgac ggccacccct tcaagctgcg tctcgccccg
      901 cgccccggcg gcggcaccga cggccggctg atggtccggc accgcgactg agcagggtgt
      961 aatggccgcc ctcgccgagc cggacgaggt gcggctccac ctcgtcgccg ccgccagggc
     1021 cgggaccgcg ctcagctatg gcgagctgct cgggcagctc ggctattcct tctcccgccc
     1081 caagatgcgc gcgctctgcg cgatcctcgg cgcgatcgac gaggccgccg agcggcgtgg
     1141 cgagcccgag cttgcggtgc tggtcgtccg ccagagcgac ggcctgccag gccagggctg
     1201 gtgggccgcc ggcggagcgc gctcccgcgg ctacgaaggg ccctgggacg gacccgaggc
     1261 caggcggttc atcgaggagg tgcaggcgga aagcttcgcc ttctggcagt cccgaagcgg
     1321 gacaggcacg ggcgaagccc cggattaacc atctctcgtc acccattttg cgcgcggatg
     1381 cggcatagcg gcggcttcaa ggagtccgcc cgatgagaga ccgcgccttt tccatgcttc
     1441 gactcagcgc cctggcgggg gtgctcgcgc tgtccgcctg caactggctc cggagcgacg
     1501 acaaggcggt cgccgccgcc aacgacgccg accccgagat cgccaacgcg ctcaacgacc
     1561 agatcctcgt cgacggcaat ctcgacaatc agtccaaccg caacggcggc cgagctccgg
     1621 tggcgcccgg caatgcgcgc tacccgtcgg cggacggcgg cgcgtccggc ggcgccaagg
     1681 ggcccgacgc ggtcgcggcg ctcggcaaga actgctccga cgcggcgcgg ttcgattaca
     1741 acatgggctg ggcgaagcgg cttgccccca atttccccgt ctatccgggc gggcgcgtga
     1801 ccgaggcggc ggcgaacaac tcgtccggct gcagcacccg cgtcgtcacc ttcaccaccg
     1861 atcacgactg gcagcgcatc ctcgactggt accatacgag ggcggtgcgg gccggctact
     1921 cctcagagca ccagctgcgc gacggcgacc acgtcctcgc cggcgccaac gccaaggaca
     1981 acaccgcctt ctacctgatc gtcacgccca agccgaaggg atcggaagtc gcgttgatcg
     2041 ccaacaacgg ccgctgaatg ccccctcgcc ccggcgaagg ctggggcgcc ggcgggtgac
     2101 aggcgccatg tccttcaccc gccgggatgc cagcctgcgc cggcatgcgg agtgggtcct
     2161 tccttcaccc gtcccgtatc gctacatcgc cgccgatgag cgaggtccgc cagttcacgg
     2221 tcgcgcccga cgacgacggc atccgcctcg atcgctggtt caagcgccat ttgcccgaaa
     2281 cgagcttcaa cacggtgtcg cgctgggcgc ggaccgggca gctgcggctg gacgggaagc
     2341 gggcggcgcc gggcgaccgg atcgaggccg ggcaggcgat ccgggttccg cccgccgaac
     2401 cggcggccgc ttcggcctcc tccgcgcggg cgaagccgga acggccgcgg ctgagcgacg
     2461 acgagatcgc cttcgtcaac gaaatcgtca ttcatcgcga tccgcacgcg atcgtggtca
     2521 acaagccgcc cggcctcgcc acccagggcg gcaccgggac ccgcacccat ctcgacggcc
     2581 ttctcgacgg tctcgccgac gaggccggcc accgccccaa gctggtccac cggctcgaca
     2641 aggacacgtc gggagtcctc ctcctcgccc gcaccgcccg ggccgccgcc ttcttctcca
     2701 aggccttttc cggccgcacc gcccgcaagg tctattgggc gatcgtgacc ggggttcccg
     2761 acatcgccga cggctatatc gatctgccgc tcgccaagca gccgggcacg ggcggcgaga
     2821 agatgcacgt cgaggaaggg gagagcggct tgccgacgcg gacccgctac cggatgatcg
     2881 agcgcgccgg caaccgcgcc gcctggctcg agctgcagcc gctgaccggg cgcacccacc
     2941 agctgcgcgt ccacatggcc gcgatcggcc atccgatcct gggcgacggc aaatatggcg
     3001 ggccggacgc cttcctgacc gggacgatca gccgcaagct ccacctccac gcccggcgcc
     3061 tccggataga ccatccggac ggcggccggc tcgacgtcac ggcggagctg ccggcccatt
     3121 tcgcggagac catggccaat ctcggcttcg acccggcgct gggcgacctg ccgctcgacg
     3181 aggtcaagtt ctccgagact gcggagggca agcgccgcgc cgcgtcggcc gccgccaagt
     3241 cgcggcgcag ggagcgcaag ggcgagcggc ggggaagggg gcgcggctga tgcatccgaa
     3301 ccgcaagttc catatcgccg acagggaagc gatggccggg ctggtgcgca gcctcggctt
     3361 cggcgtgctg ttcgtggtca ccggcgaagg gccgcgcgcg gtccatgtgc cggtgctgct
     3421 cgacggcgag cggctgcgct tccacgtctc gcgcggcaat ctcgtccact cggccctcgc
     3481 ggccggaggc acggccttgt tcgtcgccac gggtccgcac gcctacatca gcccggaatg
     3541 gtacggcctc gacgaccgcg tcccgacctg gaactacgtg gcggtcgagc tggaggggcc
     3601 ggtccggacg ctgacccccg acgagttggt cagcctggtc gacgacctct ccgcggagca
     3661 ggaggcgcgg ctcgcgccca agcccgcctg gacccgcgcc aagatgagcc ccggccgctt
     3721 cgagggcctg ctcaaggcga tcaccggctt cgagatggag gttgtcgaat ggcggggcac
     3781 cgccaagata gaccaggaca agcccgaggc ggtgcgttcg agcgtcgccg acgcgctcgc
     3841 cgcgcggggc gagcgggaga tggccgagac gatgaggaag gcagccccat gactgcgcag
     3901 gacgacgaga ccttgtggcg caaccgcttc atcgcgatca acctggtgag gatcggcggg
     3961 acgatcgtgg tcctcatcgg actcgccatc tggcacagcg atttcctggt cgagggcgga
     4021 tcgatcgcgg tcggcctgcc gatggcgctc gccggcctgg tcgccagctt ctgggggccg
     4081 aaatggctgg cggcccgctg gcggactccg cccgggccgt gaggcgcttc tacggccagg
     4141 ctgccgcctc ccccgaggag ggcggggttg ccgtgctgct cgacgg
//
LOCUS       DASVQZ010000410         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_760749, whole genome shotgun sequence.
ACCESSION   DASVQZ010000410 DASVQZ010000000
VERSION     DASVQZ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760749"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VF547_11575"
     CDS             complement(<1..633)
                     /locus_tag="VF547_11575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8263504.1"
                     /translation="MLLVLSFSLTARPAMAQSILRDAETESVFKEMSRPLIEAAGLRP
                     ENVQIVLIQDKSINAFVAGGQIVYIHSGLIAAADNANEVQGVIAHELGHITGGHIIRF
                     GEGARVAVGITLLSLLLGAAAMAAGAGEAGAAILGAGQEAAMGKFLAYTRTQESAADQ
                     AGASFLNKAGISGKGSLSFFKKLQNQEFRLALSQEDSYARSHPLSGERISA"
     gene            complement(783..>2290)
                     /locus_tag="VF547_11580"
     CDS             complement(783..>2290)
                     /locus_tag="VF547_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX8263505.1"
                     /translation="RYGAEDQLAAMYQPVVQLKSGGYLVINPTEALVSIDINSGRSTR
                     EHNIEQTAYATNIEAAHEIARQLRLRDMAGLVVIDFIDMESNGHIRKVEKAMKEALKN
                     DRARIQVGRISSFGLMEMSRQRLRTGVLEASTKPCQHCEGTGLMRTAASAGLSALRMI
                     EDEAGRGRGSRIILRAGREAAIYVLNKKRHEIAEVEERYGVLVEVLIDEGFEGARMSV
                     ESVGPPPAQRPRFEPIAAPEVDEEEDLAEELDEEEEEEEEEPAAEEERRPRQERRPER
                     ERAEAEGDRGRKRRRRRGRRGGRRREGEEGSEGEYREDRAGEGSEDAPEQVEQPVAEE
                     AAAEAVAAAEPVSEAPAPEAATVEEEQPRKTRRRPRARKKGDEQPVEALEAPAPEAPV
                     AEAPAAETPAAEAPAEAAGEPAPKPKRTRRRKKDEAVEAAPAAEEAAPAATPEAAPAA
                     PAAAPEAANDAAAGETDAPVLERPAGGEEAEAGADGSSPPRRGWWQRTFGA"
BASE COUNT          277 a          823 c          804 g          386 t
ORIGIN      
        1 cgccgagatg cgctcgcccg acagcgggtg gctgcgcgcg tagctgtcct cctgcgagag
       61 ggcgaggcgg aattcctggt tctgcagctt cttgaagaag gagagcgagc ccttgccgct
      121 gatcccggct ttgttgagga agctcgcgcc tgcctggtcg gcggccgatt cctgcgtgcg
      181 ggtgtaagcg aggaacttgc ccatcgccgc ttcctggccc gcaccgagga tcgcggcccc
      241 cgcctcgccc gcgcccgccg ccatcgcggc ggcgccgagc agcagcgaca gcaaggtgat
      301 gccgaccgcg acgcgagcgc cctcgccgaa gcggatgatg tggccgccgg tgatgtggcc
      361 gagctcgtgg gcgatcacgc cctgcacctc gttggcattg tcggcggcgg cgatcagccc
      421 cgaatgaatg tagacgatct gcccgccggc gacgaacgcg ttgatcgact tgtcctggat
      481 gagcacgatc tggacgttct cgggacgaag ccccgccgcc tcgatcagcg ggcgggacat
      541 ctccttgaag acgctctcgg tctcggcatc gcgcaggatc gactgggcca tggcggggcg
      601 cgcggtgagc gagaagctca gcaccagcag catcacaagg cggacgagga acgaggcccc
      661 ccgcgggatc gaacgatgcg cttcagccat gcggcggtat tccttgcggc gcctgaacgg
      721 gcgatgaaac cgcgcctccg gccgctccgc ccggggcggg gcttgcggag gcgcggttca
      781 tgtcaggctc cgaacgtgcg ctgccaccag ccgcggcgcg gcggcgacga gccgtcggcg
      841 cccgcttcgg cctcttcgcc cccggcggga cgctccagca cgggcgcgtc ggtctcgccc
      901 gccgcggcgt cgttggcggc ttccggcgca gcggcaggcg cggccggagc ggcctccggg
      961 gtagcggccg gagcagcttc ctcggccgcc ggagcggcct cgacggcctc gtccttcttg
     1021 cggcggcggg tgcgcttggg cttgggcgca ggctccccgg ccgcctcggc aggcgcctcg
     1081 gcggcaggcg tttcggctgc aggagcctcg gctacgggag cctcaggcgc gggagcctcg
     1141 agggcttcca ccggctgctc gtcgcccttc ttgcgggcac gcgggcggcg gcgggtcttg
     1201 cgcggctgct cttcctccac cgtcgcggct tcgggagcgg gtgcctcgga gacaggctcc
     1261 gcagccgcga cggcttccgc cgctgcttcc tcggcaaccg gctgctcgac ctgctcgggc
     1321 gcgtcctcgg agccctcgcc ggcccgatcc tcgcgatact cgccttcgct gccctcctcg
     1381 ccttccctgc gacggccacc gcggcggccg cgacggcggc ggcgcttgcg gcccctgtcg
     1441 ccctcggcct cggcccgctc gcgctccggc ctgcgctcct ggcgcggccg gcgctcttcc
     1501 tcggctgccg gttcctcttc ttcctcttcc tcttcctcgt ccagctcctc ggcgaggtct
     1561 tcctcctcgt cgacttcagg agcggcgatc ggctcgaagc gcgggcgctg ggcaggcggc
     1621 ggaccgaccg actcgacgct catcctggcg ccttcgaagc cctcgtcgat cagcacttcg
     1681 acgagcacgc cgtagcgctc ctcgacctcg gcgatctcgt ggcgcttctt gttgagcacg
     1741 tagatggcgg cctcgcgccc ggcgcgcaga atgatgcggc tgccgcggcc gcgcccggcc
     1801 tcgtcctcga tcatccgaag cgcggagagg ccggccgacg cggcggtgcg catcagcccg
     1861 gtgccttcgc aatgctggca cggcttggtc gaggcttcga gcacgccggt gcgcagccgc
     1921 tggcggctca tctccatcag tccgaaagac gagatacggc cgacctggat gcgggcgcga
     1981 tcgttcttca gcgcctcctt catcgccttc tcgaccttac ggatatggcc gttggattcc
     2041 atgtcgatga agtcgatgac gacgaggccg gccatgtcgc gtaggcgaag ctggcgggcg
     2101 atctcgtgcg ccgcctcgat gttggtcgcg taggcggtct gctcgatatt gtgctcgcgg
     2161 gtcgaacggc ccgaattgat gtcgatcgag acaagggcct cggtcgggtt gatcaccaga
     2221 tagccgcccg acttgagctg gacgaccggc tgatacatcg ccgcgagctg gtcctcggcg
     2281 ccgtagcgct
//
LOCUS       DASVQZ010000411        11041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_281029, whole genome shotgun sequence.
ACCESSION   DASVQZ010000411 DASVQZ010000000
VERSION     DASVQZ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11041
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281029"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="pspF"
                     /locus_tag="VF547_11585"
     CDS             <1..927
                     /gene="pspF"
                     /locus_tag="VF547_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HEX8263506.1"
                     /translation="ERGTGKELIAERLHHLSSRWDGPLVAMNCAALPETLIEAELFGH
                     EAGAFTGATKARAGRFEEADGGTLFLDELATLSQAAQERLLRVVEYGEVTRIGASRPL
                     TVDVRIVAATNEDLPRLAERNRFRADLLDRLSFEVVTLPPLRHRSSDIPVLADHFARR
                     MALELEWPSWPGFSNGAMDRLLNYTWPGNVRELRNVVERAVYRWEDHERPIDRIQFDP
                     FESPWRPQPPSQAAPAEALVPAPAPAGAPAAGSWDPESCADFRLAVAEYEKAILAAAL
                     EKCRWNQRAAAAALSLSYDQLRHAMKRHQLQT"
     gene            complement(930..2564)
                     /locus_tag="VF547_11590"
     CDS             complement(930..2564)
                     /locus_tag="VF547_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263507.1"
                     /translation="MAIWDSSLDIRRPIQPTWPQGMAIADLPAENSAEAGVALTHFGA
                     NVRVEPVRWDPSGARLLLRVGKVGAALWELESSRLVPAPGFDPLFTWITLEPLSYGAI
                     AFYRDPATLTLARRIQAEGDPLRWVANPGPRQTRFLVFRPGQRLELRGYENGRSWNTH
                     VPLAFADRPLLPANSKKPTFLGDQTGSRHFLPYARPVIDQVTGRVVGRFGLERIELEN
                     GRRIDLQRVFHGLTTVTDASANRDSIFALVDLAHQKRIARIRGHRIESWRLCEKSGLR
                     LGPVTLPPDDALPPNIEVMREKVFFGSGKGRPFGLLYRPRRADGRLVVWMHGGPTATL
                     ADQTVPREVYKLAPLGISVLAVEQSGMLGGGLELTRRLPRLGHAALVQDMRSVAGWAR
                     RSGYRRVFLIADSFGGASGVIAAVDHPDVYEHIFLRAPMLALRAPEKSVRGRAIDILG
                     ETAPSPGRQREFEEIVLGGRERFAAALKRWTARLRPSPRLSFYFGDFDPVSAADDLPA
                     VFAGHPSVKVVGGSHETTAGSGKVQRDIVAKLQRAN"
     gene            complement(2768..3124)
                     /locus_tag="VF547_11595"
     CDS             complement(2768..3124)
                     /locus_tag="VF547_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263508.1"
                     /translation="MIDAPTEMMTSSSISYLDFPSPVAGGFQGDTFIEGGGGGGGGVD
                     YLVGPNRWDLARDGRAIQSNGYVLIDYDGDHHYDQAWYADGDGVWRTSTGGGIWTVDS
                     GPVDELMDLWERGFVL"
     gene            3371..3919
                     /locus_tag="VF547_11600"
     CDS             3371..3919
                     /locus_tag="VF547_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263509.1"
                     /translation="MRSKFLLAAAALLAASPAAAAVTVLGNSSARQCYEAAELRGNPS
                     LDVIRTCDQALRDEGLSEYDRVATLVNRGILKARMGKLDQAIIDYDAALDRDPNEAEA
                     YLNKGFALLRLSNAAQDAKPLFDSALAKRTRRPELAYYGRAVANELSGQVRAAYEDYR
                     QASLLSPSWKDPKADLARFSVK"
     gene            complement(3945..4712)
                     /locus_tag="VF547_11605"
     CDS             complement(3945..4712)
                     /locus_tag="VF547_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8263510.1"
                     /translation="MTFNRFGVWAIYRFEMARALRTLWQSLVTPVITTSLYFVVFGAA
                     IGSRMTEVQDVPYGAFIVPGLIMLSLFTQSIFNASFGIFFPKFTGTIYEILSAPISPL
                     EIVIGYVGAAATKSVALGLVILGTAAFFVPLQILHPAWMLAFLLLIATTFSLFGFIIG
                     IWGKNFEQIQFVPMLIVTPLTFLGGAFYSVDMLPEPWRTATLFNPVVYLISGFRWSFY
                     GVGDVPVAISLGATLGFLLLCLVAVAWIFRTGYRLKT"
     gene            complement(4768..5703)
                     /locus_tag="VF547_11610"
     CDS             complement(4768..5703)
                     /locus_tag="VF547_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8263511.1"
                     /translation="MPDSPVVSIRNLSKRYAGGHEALKDVSLDIARGEIFALLGPNGA
                     GKTTLISIVCGIVTASSGEVEVDGHDIVRDYRAARSCIGLVPQELHTDAFESVWSTVS
                     FSRGLFGKPPSPAHVEKVLRDLSLLDKRKARIMELSGGMKRRVMIAKALSHEPDVLFL
                     DEPTAGVDVELRRDMWALVRRLRESGVTIILTTHYIEEAEEMADRVGVISRGELIVVE
                     EKATLMKKLGKKELTLNLSEVMDEIPPELAGWNLRLKADGHELEYVFDAQAERTGVPS
                     LLGQLRDLGIGFKDLNTRQSSLEDIFVSLVHERGR"
     gene            complement(5815..6441)
                     /locus_tag="VF547_11615"
     CDS             complement(5815..6441)
                     /locus_tag="VF547_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX8263512.1"
                     /translation="MSAVAEKKEPSVFGRLMIDLGPLLVFFLVNFLAPVPDVLRIFYA
                     TGAFMAAMIVAMMLSYLRYRHISPLLWFSGIMVVILGGVTIWLHNDVFIKMKPTVYYL
                     FIASLLGFGLMTGRNLLKMVLGAAYPGLSEQGWRLLTRNWAVFFVVMALVNEAVWRSS
                     STSFWIGFKLWGFLPATFLFAIANVPMLMKHGMQLEPGKDEPPVPPVQ"
     gene            complement(6438..7367)
                     /gene="ftsY"
                     /locus_tag="VF547_11620"
     CDS             complement(6438..7367)
                     /gene="ftsY"
                     /locus_tag="VF547_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX8263513.1"
                     /translation="MSEASWLDRLQAGFRKTSDRLGDNLTGLFTKAALDAETLDEIEE
                     ALIASDLGPETAAKIRARLAAERFERGLSEYAVKEIVAEELEKILAPVAVPLQIDAFP
                     RPQVILVVGVNGSGKTTTIAKLGHLLLEQDYGVLLAAGDTFRAAAIEQLRIWADRIGV
                     PVIAGPEGGDSSAIVFDGVKKGTAEGIDTLIVDTAGRLQNKKVLMDELAKIRRVLGRL
                     NPEAPHNVVLVLDATTGQNALSQIEVFREVAGVTGLVMTKLDGTARGGVLVAAAERYG
                     LPIHAIGIGEGIDDLRPFDARETARAIAGLGRE"
     gene            complement(7364..8668)
                     /gene="mtaB"
                     /locus_tag="VF547_11625"
     CDS             complement(7364..8668)
                     /gene="mtaB"
                     /locus_tag="VF547_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX8263514.1"
                     /translation="MSGPELITLGCRLNAAESEAMRTLAGAEDDLIIVNSCAVTNEAV
                     RQTRQAIRRAKRARPQARVVVTGCAAQIEPETFAAMPEVARVVGNAEKFDAASFVTPA
                     KAGVHAKSAWMPAFAGMTREVDARVSDIMAVRQTAPHLAAGFAERSRAFVEVQNGCDH
                     RCTFCIIPYGRGNSRSVPAGLVVERIGALAAQGFREVVLTGVDVTSYGPDLPGSPTLG
                     MLVERILRHVPELPRLRLSSLDCIEIDGRLEEIIAHEPRFMPHLHMSFQAGDDLILKR
                     MKRRHSRAQAVATVARLKAKRPELAIGADLIAGFPTETEEMHSNSLALIEECDIVMGH
                     VFPFSPKKGTPAARMPPVPAPAVKERARRLREACARRKAAWLEALVGSAQRVLVEKDG
                     SGHAENFAPVRLRHRSESWADGEIVEVKITAVENDSLVGIPA"
     gene            complement(8665..9456)
                     /gene="dapF"
                     /locus_tag="VF547_11630"
     CDS             complement(8665..9456)
                     /gene="dapF"
                     /locus_tag="VF547_11630"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX8263515.1"
                     /translation="MKRQFLKMHGLGNDFAVFDAREAPLEMDSDRARALADRRTGIGC
                     DQLILLEPSAAADVRMRIWNSDGGEVEACGNASRCIALLLGGETKIETAGGILAASAN
                     GGAATVDMGRPRFAWDEIPLAYPMDTAAMPVGWDELREPFAVNVGNPHIVFFVEDARG
                     VDLEALGPSIERDPLFPERINVNVASIEEGTIRLRVWERGAGLTQACGTGACATAVAA
                     ISRRLAASPVQVRLPGGTLAVEWAPGGTIRMTGPATRVFEGEVEL"
     gene            complement(9496..9897)
                     /locus_tag="VF547_11635"
     CDS             complement(9496..9897)
                     /locus_tag="VF547_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8263516.1"
                     /translation="MAVRTASCRCGALKARARGEPARISVCHCLACKARTGAAFSWNS
                     TWLEEQVETEGEAASWTRVTEDGGRWCTYSFCPTCGSTAWYRIEARPGMVTIPAGNFL
                     DPAFGEPAYSWYGERRCPWVRIETEGPPAQG"
     gene            9954..10211
                     /locus_tag="VF547_11640"
     CDS             9954..10211
                     /locus_tag="VF547_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263517.1"
                     /translation="MRLALVALLALPAASAAEAPLRPTPAAATPICRDDLRVRDARSP
                     EARRPRRLGELPPGDLTLTVVNRAGDCIEPVVVRQGYGLGR"
     gene            complement(10425..10886)
                     /locus_tag="VF547_11645"
     CDS             complement(10425..10886)
                     /locus_tag="VF547_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEX8263518.1"
                     /translation="MSDPPLILVDADACPVKEEIYKVAFRHGAAVRIVSNAHLRVPAH
                     PLIERVVVGAGFDAADDWIAERAGPQTVVVTGDILLADRCLKAGAAVIGPNGRPFTAA
                     SIGAAVATRAIMADLRAGMDGPGGGPAPFAKADRSRFLSALDEALVRLKRG"
BASE COUNT         1791 a         3850 c         3677 g         1723 t
ORIGIN      
        1 gagcgcggca ccggcaagga gctgatcgcc gagcgcctcc accatctctc ctcgcgctgg
       61 gacgggccgc tggtggcgat gaactgcgcg gccctgcccg aaaccctgat cgaggccgaa
      121 ttgttcggcc acgaggccgg cgccttcacc ggcgcgacca aggcccgggc gggccggttc
      181 gaggaggcgg acggaggcac cttgttcctc gacgagctcg cgaccctcag ccaggccgcg
      241 caggagcggc ttctgcgcgt cgtcgaatat ggcgaggtga cccggatcgg ggcgtcgcgg
      301 ccgctgacgg tcgacgtgcg catcgtcgcc gccaccaacg aggacctgcc ccgcctcgcc
      361 gagcgcaacc gcttccgcgc cgacctgctc gaccggctgt cgttcgaggt ggtgacgctg
      421 cccccgctcc gccaccgcag cagcgacatc ccggtgcttg ccgaccattt cgcccggcgc
      481 atggcgctcg agctcgaatg gccgagctgg ccgggcttca gcaacggcgc gatggaccgg
      541 ctgctcaact atacctggcc gggcaacgtg cgcgagctcc gcaacgtcgt cgagcgcgcc
      601 gtctaccggt gggaggatca cgagcggccg atcgaccgga tccagttcga cccgttcgaa
      661 tcgccctggc gcccgcagcc gcccagccag gcggctccgg ccgaggcgct tgtgcccgcg
      721 ccggcgccgg ccggcgctcc ggcggcgggc agctgggatc cggagagctg cgccgacttc
      781 cggctcgccg tcgccgaata tgagaaggcg atcctggccg cggcgctcga gaaatgccgc
      841 tggaaccagc gcgccgccgc cgccgccctc agcctcagct acgaccagct gcgccacgca
      901 atgaagcgcc accagctcca gacctgacgc tagttcgccc gctggagctt ggcgacgata
      961 tcccgttgca ccttgccgct gcccgctgtc gtttcgtggc tgcctccgac gaccttcacg
     1021 gacgggtggc cggcaaagac ggcgggcagg tcgtccgctg ctgaaaccgg gtcaaagtct
     1081 ccaaaataga aggacaggcg cggagaggga cgcagtcgcg ccgtccagcg tttcaaggcc
     1141 gcggcgaagc gctcgcggcc gccaaggaca atttcctcga actccctctg acggccgggt
     1201 gacggagccg tttcgcccag gatgtcgatg gcccgccctc tgacgctctt ctcgggcgcc
     1261 cgcagcgcca gcatcggggc gcgcaggaag atgtgctcgt agacgtcggg gtggtcgacg
     1321 gcggcgatga cgcccgaggc gccgccgaag ctgtcggcga tcaggaagac gcgccgatag
     1381 ccgctccgcc gcgcccaacc ggcaaccgat cgcatgtcct ggaccagggc cgcgtggccg
     1441 agtcggggaa ggcggcgcgt gagctcgagc ccgccgccga gcattcccga ttgctcgacc
     1501 gccagcaccg agattccgag cggcgcgagc ttgtagacct cgcgcggaac ggtctgatcg
     1561 gccagcgttg ccgtgggtcc tccgtgcatc cagacgacga gccggccgtc ggcccggcgc
     1621 ggacgataaa gaagaccgaa cggacgtccc ttgccggagc cgaaaaacac cttttccctc
     1681 atgacctcga tgttcggcgg gagcgcatcg tcggggggca gggtgacggg gccgagccgg
     1741 aggcctgatt tctcgcacag acgccagctt tcgatcctgt ggccccggat gcgggcgatc
     1801 cttttctggt gcgcgagatc gaccaacgcg aaaatcgagt cgcggttcgc cgacgcgtcc
     1861 gtgaccgtcg tcaacccgtg gaagaccctc tggagatcga tccggcggcc gttctccagc
     1921 tcgatccgtt caagcccgaa gcggccgacg actcgacccg tcacctggtc gatgaccggg
     1981 cgcgcgtagg gaaggaagtg cctcgatccg gtttgatcgc cgaggaaggt cggtttcttc
     2041 gagttcgcgg gaagcagagg tcggtcggcg aaggcaaggg ggacatgggt gttccacgac
     2101 cgcccatttt catagcctct caattcgagc cgttggcccg gccggaagac gaggaacctc
     2161 gtctgccgcg gccccgggtt ggccacccag cgcaggggat cgccttccgc ctggattcgc
     2221 cgcgccaggg tgagcgtcgc cgggtcgcga tagaaggcga tggcgccgta gcttagcggc
     2281 tccagcgtta tccaggtgaa gagggggtcg aagccaggcg ccggcacgag gcgcgagctt
     2341 tcgagctccc agagagcggc tccgaccttg ccgacgcgca acagcagccg tgccccgctc
     2401 gggtcccagc gcacaggctc cacgcgcacg tttgcaccaa aatgcgtaag cgctacaccc
     2461 gcttccgctg agttttcggc cggcaagtcc gcaatggcca ttccctgcgg ccaagtcggc
     2521 tggatcggcc ggcggatatc caaagagctg tcccagatcg ccacccgtac cgaatttggg
     2581 tccggaagaa tccggttccc tcggcacggc gaaatcgagc tcagtttggt cacccgcact
     2641 tcatcgccgc cgatactgac ggaagggtga ggcgggatcc ctagagccgt tgaagtggcc
     2701 gaggcgtggg caaacgcgag ggcttgaccg acgcagagga agaaagccgt cgggccgcca
     2761 cgacgcatta caagacgaat ccccgctccc ataagtccat taattcgtcc accgggccgc
     2821 tatccacagt ccagatgccg ccgccggtgc ttgtgcgcca cacgccgtca ccgtcagcat
     2881 accacgcctg atcgtaatgg tggtcaccat cataatcgat aagcacataa ccgttggact
     2941 gaattgcgcg accgtctctc gcaaggtccc atcgattggg gcccaccaga tagtcgacac
     3001 cgccgccacc accgccgccg ccttcgatga aggtgtcacc ttggaacccg ccggcgaccg
     3061 gcgaaggaaa atcgagatat gagattgaag acgaggtcat catttcagtc ggcgcgtcga
     3121 tcatagcggc cctcctcttt ccccggaacc gtcctagtca gcggtcctat ttcgtttagt
     3181 cccgcgacat cgggatttcc gtcgctctca agcgcctgaa tcttgatcgg tctggtccga
     3241 ttgcagtggg agaggagcgg gccgttattg atcgaccaag ccccggctcg atgcgaccga
     3301 gcaagccgtg gcaatggcgc tcggcacgcc attctgttat gatagaccat cttgtgaagg
     3361 aggtttcgcc atgcgcagca aattcctctt ggccgccgcg gctctgctcg ccgcatcgcc
     3421 cgccgccgcc gccgtcaccg tcctcggcaa ttcgtccgcc cgccaatgct acgaggcggc
     3481 ggagttgcgc ggcaatccga gcctcgacgt catccgcacc tgcgaccagg cgcttcgcga
     3541 cgaggggctg agcgagtatg accgggtcgc cacactggtc aatcgcggca tcctcaaggc
     3601 gaggatgggg aagctcgacc aggccatcat cgattacgac gcagcgctcg accgcgaccc
     3661 gaacgaggcc gaggcctatc tgaacaaggg tttcgcgctg cttcgccttt ccaatgcggc
     3721 tcaggacgcc aagcccttgt tcgactccgc gctggccaag cgcacgcgcc ggccggagct
     3781 ggcctattac ggccgggccg tggccaacga gctcagcggc caggtccgcg ccgcctacga
     3841 ggattatcgc caggcgagcc tgctcagccc gagctggaag gatcccaagg ccgatctggc
     3901 gcgcttctcg gtgaaatagc gcccgtcgcc ggtagcgggt cgcttcaggt cttcagccgg
     3961 tatccggtcc ggaagatcca ggcgaccgcg acgaggcaca gcagcaggaa gccgagggtc
     4021 gcgccgaggc tgatcgccac cggcacgtcg ccgaccccgt agaagctcca gcggaagccg
     4081 ctgatcagat agaccaccgg attgaacagg gtcgcggtgc gccatggctc gggcagcatg
     4141 tcgaccgagt agaaggcgcc gcccaggaag gtgagcggag tcacgatcag catcggcacg
     4201 aactggatct gctcgaaatt cttgccccag atgccgatga tgaagccgaa caggctgaag
     4261 gtggtggcga tcagcagcag gaaggcgagc atccaggccg gatggaggat ctggagcgga
     4321 acgaagaagg ccgccgtgcc gaggatgacg aggcccagcg ccaccgactt ggtggcggcc
     4381 gcgccgacat agccgatcac gatctcgagc ggggatatgg gcgcggagag gatctcgtag
     4441 atcgtgccgg tgaacttggg gaagaagatc ccgaacgagg cgttgaagat gctctgggtg
     4501 aacagcgaca gcatgatcag gccgggcacg atgaaagcgc cgtagggcac gtcctggacc
     4561 tcggtcatgc gcgacccgat cgccgccccg aacaccacga aatagagcga ggtcgtgatc
     4621 accggagtca ccaggctctg ccacagggtg cgcagcgcgc gcgccatctc gaaccggtag
     4681 atcgcccaga cgccgaagcg gttgaacgtc atgacccggc tccgagggcg gcacgcgatt
     4741 ccaaggcgag gggggcgccg agcccgctca tcggccgcgc tcgtgcacca ggctgacgaa
     4801 gatgtcctcg agcgagctct ggcgggtgtt gaggtccttg aagccgatgc cgaggtcgcg
     4861 cagctgcccg agcagggagg ggaccccggt ccgctcggcc tgggcgtcga agacatattc
     4921 gagctcgtgg ccgtcggcct tgagcctgag gttccatccc gccagctcgg gggggatctc
     4981 gtccatcacc tcgctgaggt tgagggtgag ctccttcttg ccgagcttct tcatcagcgt
     5041 cgccttctcc tcgacgacga tcagctcgcc gcgcgagatg actccgaccc ggtcggccat
     5101 ctcctcggcc tcctcgatat agtgggtggt gaggatgatg gtgactccgc tttcccgaag
     5161 gcggcggacg agcgcccaca tgtcgcggcg gagctcgacg tcgacgccgg cggtgggctc
     5221 gtcgaggaac aggacgtccg gctcgtggct gagggccttg gcgatcatca cccggcgctt
     5281 catcccgccc gacagctcca tgatccgagc cttgcgcttg tcgagcagcg agaggtcgcg
     5341 aagcaccttt tcgacgtggg cgggcgaggg gggcttgccg aacaggccgc ggctgaagct
     5401 gaccgtcgac cagacgctct cgaaggcgtc ggtgtggagc tcctggggca ccaggccgat
     5461 gcagcttcga gcggctcgat agtcccggac gatgtcgtga ccgtccacct cgacctcgcc
     5521 ggagctggcc gtgacgattc cgcacacgat gctgatcaag gtggtcttgc cggccccgtt
     5581 cggcccgagc agggcgaaga tctcgccccg ggcgatgtcg aggctgacgt ccttcagcgc
     5641 ctcgtggccg ccggcgtagc gcttcgacag gttccggatc gagacgacgg gagagtcagg
     5701 catcggtctc accaacctcg tcaccccagc gaaggctggg gtccatgccg aatccggatg
     5761 gatgccagcc ttcgctggca tgacgaagct ccgctcgcaa tgtcagtcct gagctcattg
     5821 gaccggcggc accggcggct cgtccttgcc cggctcgagc tgcattccgt gcttcatcag
     5881 catcggcacg ttggcgatgg cgaacaggaa ggtggcggga aggaagcccc acagcttgaa
     5941 gccgatccag aagctggtgc tgctgctgcg ccacaccgcc tcgttgacca gggccatcac
     6001 cacgaagaag accgcccagt tgcgggtgag cagcctccat ccctgctccg agaggcccgg
     6061 ataggcggcg ccgagcacca tcttgagcag gttccggccc gtcatcaggc cgaagccgag
     6121 caggctcgcg atgaagagat aatagaccgt cggcttcatc ttgatgaaga cgtcgttgtg
     6181 gagccagatc gtcaccccgc cgaggatcac caccatgatc cccgagaacc agagcaaagg
     6241 cgagatgtgg cggtagcgca ggtaggacag catcatcgcc acgatcatcg ccgccatgaa
     6301 ggcgccggtc gcgtagaaga tcctgaggac gtcgggaacc ggcgccagga agttgaccag
     6361 gaagaagacg agcagcggcc cgagatcgat catcagccgg ccgaagaccg aaggttcctt
     6421 cttttccgcc acggcgctca ctctcttccc agcccggcga tcgcgcgcgc cgtctcgcgc
     6481 gcgtcgaacg gccgcaaatc gtcgatcccc tcgccgatgc cgatggcgtg gatcggcagc
     6541 ccgtagcgtt cggccgcggc gaccagcact ccgccccggg cggtgccgtc gagcttggtc
     6601 atgaccagcc cggtcacgcc cgccacctcc cggaacacct cgatctggct cagcgcgttc
     6661 tgcccggtcg tcgcgtcgag caccagcacg acgttgtgcg gcgcctccgg attgagccgg
     6721 ccgagcaccc gccggatctt ggcgagctcg tccatcagca ccttcttgtt ctggagccgg
     6781 cccgcggtgt cgacgatcag cgtgtcgatg ccctcggccg tgcccttctt gactccgtcg
     6841 aagacgatgg ccgagctgtc gccgccctcc ggcccggcaa tgaccggcac gccgatccgg
     6901 tccgcccaga tcctgagctg ctcgatcgcg gcagcccgga aggtgtcgcc ggccgcgagc
     6961 aggaccccgt aatcctgctc gagcaggaga tggccgagct tcgcgatggt cgtcgtcttg
     7021 cccgatccgt tgactccgac gaccaggatc acctgcggcc gcgggaaggc gtcgatctgc
     7081 agcggcaccg ccaccggcgc caggatcttc tcaagctcct cggcgacgat ctccttgacc
     7141 gcatattcgc tgaggccgcg ctcgaaccgc tcggcggcga gccttgcccg tatcttggcg
     7201 gccgtctccg gcccgaggtc cgacgcgatc agcgcctcct cgatctcgtc gagcgtctcg
     7261 gcgtcgagcg ccgccttggt gaacaggccg gtgaggttgt cgccgagccg gtcggacgtc
     7321 ttgcggaagc cggcctgcag ccggtccagc cagcttgcct cgctcatgcg gggattccga
     7381 cgaggctgtc gttttcgacg gccgtgattt ttacctcgac aatttctccg tcagcccagc
     7441 tttcgctgcg gtgacgaagg cggacaggcg cgaaattctc cgcgtgcccg ctcccgtcct
     7501 tctcgaccag cacccgctga gccgacccga ccaaagcctc gagccacgcc gccttgcggc
     7561 gcgcgcaggc ctcgcgaagc cgccgggccc tttccttcac cgccggcgcc ggaaccggcg
     7621 gcatccgcgc cgccggagtg cctttcttcg gcgagaaggg gaagacgtgg cccatgacga
     7681 tgtcgcattc ctcgatcagg gcgaggctgt tggaatgcat ctcctcggtc tcggtcggga
     7741 agccggcgat gagatccgcg ccgatcgcca gctccggccg cttcgccttg agccgcgcca
     7801 ccgtcgcgac cgcctgcgcc cggctgtgcc gccgcttcat ccgcttcagg atcaggtcgt
     7861 cgccggcctg gaagctcatg tggaggtgcg gcatgaagcg cggctcgtgg gcgatgatct
     7921 cctcgagccg gccgtcgatc tcgatgcagt cgagcgagga gaggcgcagc cgcggcagct
     7981 cggggacgtg cctcaggatg cgctcgacca gcatgccgag ggtcggcgag cccggcaggt
     8041 cggggccgta gctggtgacg tccacgccgg tcaggacgac ctcgcggaag ccctgggcgg
     8101 cgagcgcccc gatccgctcg acgacgaggc cggcggggac gcttcggctg ttcccccggc
     8161 cgtaggggat gatgcagaag gtgcagcgat ggtcgcagcc gttctgaacc tcgacgaagg
     8221 cgcggctgcg ctcggcgaag ccggcggcga ggtgcggcgc cgtctggcgg acggccatga
     8281 tgtcggagac ccgcgcgtct acctccctcg tcatgccagc gaaagctggc atccatgccg
     8341 acttcgcatg gaccccagct ttcgccgggg tgacgaacga ggctgcgtcg aacttctccg
     8401 catttcccac cacccgggcg acctccggca tcgcggcgaa cgtctccggc tcgatctgcg
     8461 cggcgcatcc ggtgaccacc acccgcgcct ggggccgcgc ccgcttggcc cggcggatcg
     8521 cctgccgggt ctggcgcacc gcctcgttgg tgacggcgca gctgttgacg atgatcagat
     8581 cgtcctcggc gccggccagc gtccgcatcg cctcgctctc ggcggcgttg agccggcagc
     8641 cgagcgtgat cagctccggc ccgctcacag ctcgacctcg ccttcgaaga cccgggtcgc
     8701 cggcccggtc atccggatcg tcccgcccgg cgcccattcg accgcaaggg ttcccccggg
     8761 caggcgcacc tggacggggc tcgcggcgag ccttcggctg atcgccgcca ccgccgtcgc
     8821 gcaggcgccg gtgccgcacg cctgggtgag gccggcgccg cgctcccaga cccgaaggcg
     8881 gatcgtgccc tcctcgatcg aggcgacgtt cacgttgatc cgctccggga agagcgggtc
     8941 gcgctcgatc gagggcccga gcgcttccag gtcgacgccg cgcgcgtcct cgacgaagaa
     9001 gacgatgtgc ggattgccga cattgacggc gaagggctcg cgcagctcgt cccagccgac
     9061 cggcatcgcc gccgtgtcca tcggataggc gagggggatc tcgtcccagg cgaaacgggg
     9121 ccggcccatg tcgacggtcg ccgcgccccc gttcgccgac gcggcgagga ttccgcccgc
     9181 cgtctcgatc ttcgtctcgc cgccgaggag gagggcgatg cagcgcgaag cgttgccgca
     9241 cgcctcgacc tcgccgccgt ccgagttcca gatccgcatc cggacgtcgg cggcggcgct
     9301 cggctcgagc aggatcagct ggtcgcagcc gatgccggtg cgccggtcgg ccagcgcccg
     9361 agcgcggtcc gagtccatct cgagcggcgc ctcgcgcgcg tcgaagacgg cgaagtcgtt
     9421 gccgagcccg tgcatcttga ggaactgccg cttcatcgcc cggccatcta gggaggggcg
     9481 ggggtcaagt ccacctcagc cctgcgccgg cggtccctcc gtctcgatcc ggacccaggg
     9541 gcatcgccgc tccccatacc aggaataggc cggctcgccg aaggcgggat cgaggaaatt
     9601 gccggccgga atggtcacca tccccggccg cgcctcgatc ctgtaccagg cggtcgagcc
     9661 gcaggtcggg cagaagctgt aggtgcacca gcgcccgccg tcctccgtca cccgggtcca
     9721 gctcgccgct tcgccctcgg tctcgacctg ctcctcgagc caggtcgagt tccaggagaa
     9781 ggcggcgccg gtgcgcgcct tgcacgcgag gcagtggcag accgagatcc gcgccggctc
     9841 tccccgcgcc ctggccttga gcgcgccgca gcggcagctc gccgtccgga ccgccatccc
     9901 ctctcccctc gatcgtcgca aaaggctatc atagcggctc gaaaggagac gtcatgcgcc
     9961 ttgctctcgt cgcgctgctg gcgcttcccg ccgcgtcggc ggccgaggcg ccgctccgtc
    10021 ccacgcccgc cgccgcgacg ccgatctgcc gcgacgacct gagggtccgc gacgcccgct
    10081 cgcccgaggc ccggcggccc aggcggctcg gcgagcttcc gccgggcgac ctcaccctga
    10141 ccgtcgtcaa ccgcgccggc gactgcatcg agcccgtcgt cgtccgccag ggctacggcc
    10201 tcgggcgcta ggacgggtcg acatgcacaa acccgaccct ttctttcccg tctcccaggc
    10261 ggggatctag agcgtgatcg ccttccaccg aaccgctcat cctgagtagg gactgagcga
    10321 aggcgaaggc ccgtatcgaa ggaccgtcct tcgatacgcc ggttcgacta cgctcaccgg
    10381 ctactcagga tgagcgacct tggatcaacc tggcccgatc atgtctagcc gcgcttcagc
    10441 cggaccagcg cctcgtcgag cgccgagagg aatcgcgagc ggtcggcctt ggcgaagggc
    10501 gccggcccgc cgccgggccc gtccattccc gcgcgaaggt cggccatgat cgcccgagtc
    10561 gccaccgccg ccccgatcga ggcggcggtg aatggccttc cgttggggcc gatcaccgcc
    10621 gcgccggcct tgaggcagcg gtccgcgagc aggatgtcgc ccgtcaccac caccgtctga
    10681 ggccccgctc gctcggcgat ccagtcgtcc gcggcgtcga agccggcgcc gacgacgacc
    10741 cgctcgatca gcgggtgcgc gggtacgcgc aaatgcgcgt tgctgacgat cctgaccgcg
    10801 gcgccgtggc ggaaggcgac cttgtagatc tcctccttga ccgggcaggc gtcggcgtcg
    10861 accaggatca gcggcgggtc gctcatttcc gggcgaggcg ctcgtagcgc agccggcccc
    10921 cgccgcgctc gacgatcagc gccttcttct cggccctgac cgtcacgccc tcgaaatcga
    10981 ccactccgag atcgttcggc acgagggtga aggcgagggc gttgccggcc gcgaggtcga
    11041 t
//
LOCUS       DASVQZ010000412         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_854594, whole genome shotgun sequence.
ACCESSION   DASVQZ010000412 DASVQZ010000000
VERSION     DASVQZ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854594"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF547_11650"
     CDS             <1..366
                     /locus_tag="VF547_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8263519.1"
                     /translation="KALGVQIAMDDFGTGYSSLSYFRMFPFDKVKIDQSFIRDMIDNP
                     QARAIIRSVIGLGRGLGMPVVAEGVETAEQLDALRAEGCDQVQGYWISRPGPIAHFEG
                     VVMKRAANARAQAPRKQAR"
     gene            671..1216
                     /locus_tag="VF547_11655"
     CDS             671..1216
                     /locus_tag="VF547_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263520.1"
                     /translation="MRSLALLALAPLLAAAAPPVSVTPVSPAFAPAADEYRRLWTSEG
                     PAIVAAMERWAGLAYPPGAIEILVSEGRPMTAYDGRTIRLRASYSPAYKKATLVHEMG
                     HRLVLAFPRTGGLDDHRLLYLFLYDAWTDLYGRDFADRMVAIERRIGPTYEAAWSWAL
                     AMSREQRQARLAALRADPHRP"
     gene            1296..>2543
                     /locus_tag="VF547_11660"
     CDS             1296..>2543
                     /locus_tag="VF547_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX8263521.1"
                     /translation="MMRLTRRKVLGAAVLGAAAAVLPGGGRVLAQSPQPRRLPLGDFA
                     RNPKLVEALRRGVQVMRDRPGSDPRSWFWQCAVHAYSDEIYAEAKARDPAVGKVDARK
                     YWKQCPHFGQSSANFLIWHRAYLYHFERILRDAAEEPELAVPYWNYDEEGQRGFPELF
                     APQFLDRDKKVANSLHHPNRELAFVKGLFTLSEPVSAATRAREAPQFFSEPGVTGFGG
                     DILGEGTRPGAVEQRPHNDLHIAVGGVVGDSNGAMADVPTAAFDPVFWVHHANIDRIW
                     RQWAASPGKRWGPLPPDSWFDEAPWTFIDSDGSEARKPRRFYMEAENLPILYADSAAP
                     AAGLALPRGPTPPAPAAAPPQRALFQSGRSVVVSADRPAASLAAAAPRRGPVARVPGR
                     PRRPSGGNRDGVPPPPPPPPPPPP"
BASE COUNT          386 a          949 c          846 g          362 t
ORIGIN      
        1 aaggcgctcg gagtccagat cgcgatggac gatttcggca ccggctattc gtccttgagc
       61 tatttccgga tgttcccgtt tgacaaggtc aagatcgacc agagcttcat ccgggacatg
      121 atcgacaatc cccaggcccg agctatcatc cgctcggtga tcggcctcgg ccgggggctc
      181 ggaatgccgg tggtcgccga aggggtggag accgccgagc agctcgacgc gctccgcgcc
      241 gaaggctgcg accaggtcca gggctattgg atcagcaggc cgggtccgat cgcccatttc
      301 gagggggtcg tcatgaagcg cgcggcgaat gccagggcgc aggcccctcg aaagcaggca
      361 cgctagagcc tccgccgccg cccggcgaag gccgcgaccg ggccggtccc ccgccgccgc
      421 ggggtcccgg ccttcgccgg ggagcaaggt cgaaagtccc aaaagacacg catgtcgggg
      481 gcaagaatcc tttgttttca gtcgagccga ctcggccaaa gcgcagcgat cggcaaagcc
      541 gacggcgaag gcggcgacca cggaaaacag gatcagcagg gtcaatgcgc gcctccccga
      601 ttcgaagatt aggaacataa catgaaccac gaggatgggc aatcggctcg gccgagccta
      661 tgctgcgtcg atgcgctccc tcgccctcct cgcgctcgcg cccctgctgg ccgccgccgc
      721 tccgcccgtc tccgtcactc ccgtctcccc cgccttcgcg ccggcggccg acgaatatcg
      781 gcggctgtgg acgtcggaag gaccggcgat cgtcgcggcg atggagcgct gggcggggct
      841 tgcctacccg cccggggcga tcgagatcct cgtctccgaa ggccggccga tgaccgccta
      901 tgacggccgc accatccgac ttcgggcaag ctattcgccg gcctacaaga aggcgacgct
      961 ggtgcacgag atgggccacc gcctcgtcct ggctttcccg cggaccggcg ggctcgacga
     1021 tcaccgcctc ctctacctgt tcctctacga cgcctggacc gacctctacg gccgcgactt
     1081 cgccgaccgg atggtggcga tcgagcgccg gatcggcccc acctacgaag ccgcctggag
     1141 ctgggcgctg gcgatgagcc gcgagcaaag gcaggcgcgg ctcgcggcgc tccgcgccga
     1201 cccgcaccgg ccctgaacgg cctccgtgga aacctgccgg tcctgccgaa ttgcgccgcc
     1261 ctcgcaaagc tgtagattgc cgccgcgacg gggggatgat gcgactcacg cgaaggaagg
     1321 tgctcggcgc ggccgttctg ggagccgccg cggccgtgtt gcccggcggc gggcgcgttc
     1381 tggcccagtc gccgcagccc cgccggctgc cgctcggcga cttcgcccgg aacccgaagc
     1441 tggtcgaggc gctccggcgc ggggtccagg tcatgcgcga ccggccgggc tcggacccca
     1501 ggagctggtt ctggcaatgc gcggtccacg cctattccga tgagatctac gccgaggcga
     1561 aggcccgcga ccccgccgtg ggcaaggtcg atgcgcgcaa atattggaag caatgcccgc
     1621 atttcgggca gagctcggcc aatttcctga tctggcaccg agcctatctc taccatttcg
     1681 agcggatcct ccgcgacgcc gccgaggagc ccgagctcgc cgtcccctat tggaattacg
     1741 acgaggaagg gcagcgcggc ttccccgaac tgttcgcgcc gcaattcctc gaccgcgaca
     1801 agaaggtggc caattccctc catcatccca atcgcgagct cgccttcgtc aagggcctct
     1861 tcacgctcag cgagccggtc tcggcggcga cccgcgcccg cgaggcgccg caattcttca
     1921 gcgagccggg cgtcaccggc ttcggcggcg acatcctcgg cgaaggcacg aggcccggag
     1981 cggtcgagca gaggcctcac aacgacctgc acatcgccgt cggcggagtc gtcggcgatt
     2041 ccaacggcgc catggccgac gtgcccaccg ccgccttcga cccggtcttc tgggtccacc
     2101 acgccaatat cgaccggatc tggcggcaat gggcggcgag ccccggcaag cgctggggcc
     2161 cgcttccgcc ggactcctgg ttcgacgagg cgccctggac cttcatcgat tccgacgggt
     2221 ccgaggcgag gaagccgagg cgcttctaca tggaggcgga gaacctgccg atcctctacg
     2281 ccgactccgc cgcgcccgcg gcgggcctcg ccttgccccg cggcccgact ccgccggccc
     2341 cggccgcggc cccgccccag cgggccctgt tccagagcgg gcgctcggtc gtcgtctcgg
     2401 ccgaccggcc ggcggcgagc ctcgccgccg ccgccccgcg ccgcggcccg gtcgcccggg
     2461 tcccgggccg gccgcggcgg ccgtcgggcg gaaaccgcga cggggtcccg ccgccgcctc
     2521 ccccaccccc tccgcctccg ccg
//
LOCUS       DASVQZ010000413         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_184341, whole genome shotgun sequence.
ACCESSION   DASVQZ010000413 DASVQZ010000000
VERSION     DASVQZ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184341"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1019
                     /locus_tag="VF547_11665"
     CDS             141..1019
                     /locus_tag="VF547_11665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8263522.1"
                     /translation="MGDLRAAAIALYDRYTHEGLDRRAFMAELTRLAGSAGAAALLLQ
                     AIAADPAAAALVPDADSRIRARRMSWPVGGGRTMKGYQAEPRGAAAPLGSVLVIHENR
                     GLNAHTQDVARRLAVAGYRALAADFLSPVGGTPADEDSAREAIGKLDLAQSAGDSVAT
                     LRLLKSLPGSNGKAGAVGFCWGGGMVNRVALAAGELLDAGVSFYGPAPDPALAPQIRS
                     PLQLHYAELDERVNKTGLPWRDALAAAGKTVDAHVYPKVNHAFHNDTSAERYDPAAAK
                     LAWQRTLDFFGRHVAA"
     gene            complement(1206..1943)
                     /locus_tag="VF547_11670"
     CDS             complement(1206..1943)
                     /locus_tag="VF547_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX8263523.1"
                     /translation="MANGFDPRATAAPHSAGTAGRESVAFDAGLRSHMLSVYNYMASA
                     VLLTGIVAYAAAASGIAQQIFFESGILKYVVLFAPLIMVFVLAARAHRMSMGGLQAFY
                     WSFAALMGLSMSIIPLLYSGTSIAATFFATSAAFVTMSLWGYTTKRDLAPMGTFLIMG
                     VVGLIVAMLVNMFLRSDAMSMAISAIGVLLFAGLTAYDTQKIKSLYFQVAGSDMLGKA
                     AIMGALTLYLDFINMFQFLLSFMGSRD"
     gene            2066..2599
                     /gene="thpR"
                     /locus_tag="VF547_11675"
     CDS             2066..2599
                     /gene="thpR"
                     /locus_tag="VF547_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX8263524.1"
                     /translation="MHRLFVAIRLPAPVRAQLLDLMGGVAGARWLPDDQLHLTLRFIG
                     EVDRHLARDVDAALSSVHHPGFSISLNGLGAFERRGEPVILWAGITPHEPLRALHKKV
                     DQALVRVGVEPDRRAYMPHITIARLPRGAGPVGSLVERSGGVAGPPFAVSQFCLYESR
                     LSPDGPIYSVAGLYSLD"
     gene            complement(2600..3409)
                     /locus_tag="VF547_11680"
     CDS             complement(2600..3409)
                     /locus_tag="VF547_11680"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX8263525.1"
                     /translation="MTAFARRVAALTGVGEERIERLAGGDLSEALLIRRPDGRLSVAK
                     RALSTGAEAAMLRAMAAAGLPAPVVEGEHEGLLLLEHVANDRVFSPAAWADVGAWLRR
                     LHNQAGEQYGWPVDYQIGTVALDNRERRDWPGFWAEQRLAATAAVLDRPWRERVASLV
                     RNIGEIAPQSPRPSLLHGDLWGGNILVSQGRLAALIDPACYHGDAEVDLAMLTLFDSP
                     GETFWESYGPLEPGWEERRPLYQLFPALLHMRLFGETYAGMVDRLLGRLGV"
BASE COUNT          581 a         1220 c         1164 g          520 t
ORIGIN      
        1 gttcggaacc atcgacaggc gcccgtcctt caccgaatat ttcgagcgga tcagcagccg
       61 gccggcggcg atccgggcgc gcgagatgga cgatgcgctg atgccggaac agtcgctccc
      121 gcaatcgcag gaaagctgag atgggcgatc ttcgcgctgc cgccatcgcc ctttacgacc
      181 gctacaccca tgaaggtctg gaccgccgcg cgttcatggc ggagcttacc cgcctcgccg
      241 gaagcgccgg agccgccgcg cttctgctcc aggccatcgc cgccgatccg gccgcggcgg
      301 ccttggtccc ggatgccgac agccgcatcc gggcaaggcg gatgagctgg ccggtcggcg
      361 gcggccgcac gatgaaaggc tatcaggccg aaccgcgcgg ggcggccgct ccactgggtt
      421 cagtgctcgt catccacgag aatcgggggc tcaacgccca tactcaggac gtggcccgcc
      481 gcctcgccgt cgccggctat cgagccctgg ccgcggattt cctctcgccg gtcggcggaa
      541 cccccgccga cgaggactcg gcgcgcgaag cgatcggaaa gctcgatctg gcccagtcgg
      601 cgggcgactc ggtggcaacg ctgcgcctgc tgaagtcgct tccggggagc aacggcaagg
      661 cgggcgccgt cggcttctgc tggggcggcg ggatggtcaa ccgagtggcc ctggccgcgg
      721 gcgagctgct ggacgccgga gtctccttct acggcccggc tccggacccg gcgctcgcgc
      781 cgcagatacg ctccccgctg cagctccatt atgccgagct tgacgagcgg gtgaacaaga
      841 ccggcctccc ctggcgggac gcgctcgccg ccgccggcaa gacggtcgat gcccacgtct
      901 atcccaaggt caaccacgcc ttccacaacg acacctcggc ggagcgttac gacccggccg
      961 cggcgaagct cgcctggcag cgcaccctcg acttcttcgg ccggcacgtc gctgcttgat
     1021 cctccccgtc gcgaagcgat ggggaggggg accacccgaa gggtggtgga ggggtgctgg
     1081 cgaggcctgg gagctacccc tccaccatgc ttcgcatggt ccccctcccc atgctccgca
     1141 tggggaggat aaaaaaaagg cccggcgttt ccgccgggcc ttctctttgc cgaagcaggc
     1201 attcgctagt cgcggctgcc catgaagctc agcaggaact ggaacatgtt gatgaagtcc
     1261 agatagagcg tcagggcgcc catgatcgcc gccttgccga gcatgtccga gccggccacc
     1321 tggaagtaga ggctcttgat cttctgcgta tcgtaggcgg tgaggccggc gaacagaagc
     1381 acgccgatcg cgctgatcgc catgctcatc gcgtccgagc gaaggaacat gttgacgagc
     1441 atcgcgacga tcagcccgac cacgcccatg atcaggaagg tgcccatcgg cgccagatcc
     1501 ctcttcgtgg tgtagcccca caggctcatc gtcacgaagg cggccgaggt ggcgaagaag
     1561 gtcgccgcga tcgaggtccc gctgtacagg agcgggatga tcgacatcga cagccccatc
     1621 agagcggcga acgaccagta gaaggcctgg agcccaccca tcgacatgcg atgggcgcgt
     1681 gccgcgagca cgaagaccat gatcagcggc gcgaacagaa cgacatattt gaggatgccg
     1741 ctctcgaaga agatctgctg ggcgattccg ctcgccgccg ccgcataggc gacgatcccg
     1801 gtgagaagca cggccgaagc catgtaattg tagaccgaca gcatgtggga ccgcaggccc
     1861 gcgtcgaagg cgacgctttc gcggccggcc gtaccggccg aatgcggcgc ggcggtcgcg
     1921 cgcgggtcaa atccgttagc catttcgaac tctctccttt cgcggcgaaa ctcgagcgcc
     1981 gcgtcgcccc aatatcgtga gatgatggcc catattcaag gaaaaccggt acgcacgccg
     2041 ctccggcgcc gctaaggagc gcggcatgca ccgcctgttc gtcgcgatcc gccttccggc
     2101 gccggtccga gcgcaattgc tcgacctgat gggcggggtc gccggcgcgc gctggctgcc
     2161 ggacgatcag cttcatctga ctcttcggtt catcggggag gtcgaccgtc atctggcgcg
     2221 cgacgtggat gccgcgctca gttccgtcca ccatcccggc ttctcaatct cgctgaacgg
     2281 cctcggcgcg ttcgagaggc gcggggagcc ggtgatcctg tgggcgggaa tcactcccca
     2341 cgaaccgctc cgcgcgctcc acaagaaggt cgaccaggcg ctcgtcaggg tcggagtgga
     2401 gccggaccgg cgggcctaca tgcctcacat cacgatcgcc cgcttgcccc gcggcgccgg
     2461 ccccgtcgga tcgctggtcg agcggtcggg cggggtggca gggccgccct tcgccgtttc
     2521 ccaattctgc ctgtacgaga gccgcctctc ccccgacggg ccgatctact cggtggccgg
     2581 gctctactcg ctcgactgat cagactccca gcctgcccag gaggcggtcg accatgccgg
     2641 cgtaagtctc cccgaaaagc cgcatgtgga gcaaggcggg gaacagctgg tagagcgggc
     2701 gccgctcctc ccagcccggc tcgagcggcc cgtacgactc ccagaacgtc tcgccgggac
     2761 tgtcgaagag ggtgagcatc gcgagatcga cctcggcatc gccatgatag caggccgggt
     2821 cgatcaaggc agcgagccgg ccttgagaga cgaggatgtt gccgccccac aggtcgccgt
     2881 ggaggagtga cggccgaggc gattggggcg cgatctcgcc gatgttgcga acgaggctgg
     2941 cgacccgctc ccgccagggc cggtcgagta ccgccgcggt agccgccagc ctctgctccg
     3001 cccagaagcc cggccagtcc cgccgctcgc gattgtcgag cgcgacggtg ccgatctgat
     3061 agtcgaccgg ccagccatat tgctccccag cctggttgtg gaggcgccga agccaggcgc
     3121 cgacatcggc ccaggcggcg gggctgaaca cccggtcgtt cgcgacatgc tcgagcagga
     3181 gcagaccctc atgctccccc tccacgaccg gcgccggcag tcccgctgca gccatcgccc
     3241 gaagcatcgc cgcctcggct ccggtggaaa gggcacgctt ggcgacggac aagcgcccgt
     3301 ccgggcgccg gatcagcagg gcttccgaaa gatcgccgcc ggcaagccgc tcgatccgct
     3361 cctcgccgac cccggtgagg gccgccactc gccgggcgaa agcggtcatc cgccggcggc
     3421 gatccgccgc gccagggcct cggcgccggc gctcacctca tcccaggccg tgtcgaagtg
     3481 gctgt
//
LOCUS       DASVQZ010000414         2756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_84282, whole genome shotgun sequence.
ACCESSION   DASVQZ010000414 DASVQZ010000000
VERSION     DASVQZ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84282"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VF547_11685"
     CDS             complement(<1..430)
                     /locus_tag="VF547_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263526.1"
                     /translation="MTNAELWALKIWLVVTVLAGISLFFGLIVLLLIFGLVTIPLALL
                     ITLMPTVWIYLTPALLLYALLRLTLRRRPVRPWILAFGSAAVAAAAGFAVPTLANREI
                     ERRADRIMAADMGEEPLLAPVGTVALVDDGLGRDGKCWDQC"
     gene            complement(481..579)
                     /gene="ffs"
                     /locus_tag="VF547_11690"
     ncRNA           complement(481..579)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF547_11690"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(744..1625)
                     /gene="bla"
                     /locus_tag="VF547_11695"
     CDS             complement(744..1625)
                     /gene="bla"
                     /locus_tag="VF547_11695"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX8263527.1"
                     /translation="MIDRRTFLAGALAAGACARPVPAASPPGDAAERFEAIRATLGPG
                     GRLGVAAIDTGSGRELRFDADSRYGMASTFKLPLAGFVLKLVERGEASLDEELPFGPD
                     DPLDNSPLVAANRARGRLPLRQLCAAIIEVSDNSAANILLRRTGGPEALTRFIRACGD
                     PVTRLDRFEMALNSNLPGDPRDTTSPAAMAGLTRRLVLGDLLAEENRNRLSLWLKKSV
                     PGPDRLKAGLPAPPWLVGHKTGTGANGNVNDVAIAWRSGKPPIVIACYQSGGTAEQPV
                     RFAAHAAVGKLVAEQFA"
     gene            complement(1627..1962)
                     /locus_tag="VF547_11700"
     CDS             complement(1627..1962)
                     /locus_tag="VF547_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8263528.1"
                     /translation="MTLVRFVSADGNHVQEVRGRPGERLLDLAQAHGQPLEGTCEGQM
                     ACSTCHVIVAAEDFAKLPRAGEEEEDLLDLAARVTRTSRLACQIWLDETLDELTVRMP
                     GESHDMQGR"
     gene            complement(1959..>2756)
                     /locus_tag="VF547_11705"
     CDS             complement(1959..>2756)
                     /locus_tag="VF547_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8263529.1"
                     /translation="LASLGFDSEILPVAPDGLVDLAAVEAALATPTGLVAAMLVNNEI
                     GVIQPVDRIGKMARAAGALFLCDAVQGFGRVPVPLGDCDLVAVSAHKVHGPKGIGALW
                     VRGGVELDPLLHGGGQEGGLRSGSLSPALCVGFGEAARLLVERGEEDRAHVERLWAKG
                     RAQMAGWTLNGSDDRRYRGNLNLRRDGVDAARLISELRGIAFSAGSACASGSGRPSHV
                     LAALGLADREARSSLRLGFGRYTTGEELAEALALIDSAASRQPGLAA"
BASE COUNT          417 a          943 c         1053 g          343 t
ORIGIN      
        1 ggcactggtc ccagcatttc ccgtcgcggc ccaggccgtc gtcgacgagc gcgacggtcc
       61 cgaccggcgc cagcaacggc tcctcgccca tgtccgcggc catgatgcgg tcggcgcgcc
      121 gctcgatctc gcggttggcg agggtcggaa cggcgaagcc ggcggcggcg gcgacggcgg
      181 cggagccgaa ggcgagaatc cacggtcgga ccggccgccg ccgcagcgtg agccgaagca
      241 aggcgtacag gaggagcgcc ggggtcaggt agatccagac cgtcggcatc agcgtgatca
      301 gaagcgccag cgggatcgtg accaggccga agatgagcag caggacgatc aggccgaaga
      361 acaggctgat cccggcgagc accgtcacca ccagccagat cttgagcgcc cagagctcgg
      421 cgttagtcat gagcgcgagg ctagcacggc cttcgcggcc cagaaacgcc ctgtcgggaa
      481 cgggtgggag ccggaaacga cccggcgctt gaactcgtta cggctgcttc cttccggacc
      541 tgaccgggtt ggcgagagcg ccgtccgccc gactcccgcg gcgcatatgg tggaagcgac
      601 gcatatgcgc aagccgccgc aacccctctc cctgcagggg agagggacag ccgcgaagcg
      661 gcagggtgag ggaccgaact tgccctcacc ctcccaagct tcgcttgggc cccaccctct
      721 cccctgcagg gagaggggat cgatcaggcg aactgctccg cgaccagctt cccgacggcg
      781 gcgtgagcgg cgaagcggac gggctgctcg gcggtgccgc cgctctggta gcaggcgatg
      841 acgatcggcg gcttgcccga gcgccaggcg atagccacgt cgttgacatt gccgttggcg
      901 ccggtcccgg tcttgtggcc gaccagccag ggcggcgccg gcaatcccgc cttcagccgg
      961 tccggtcccg gcaccgactt cttcagccac agcgagaggc ggttgcgatt ctcctccgcc
     1021 agcaggtcgc cgagcaccag cctgcgggtg agcccggcca tcgccgccgg gctggtggtg
     1081 tcgcgcgggt cgccgggaag gttggaattg agcgccatct cgaaccggtc gagccgggtg
     1141 acggggtcgc cgcaggcgcg gatgaagcgg gtcaaggcct ccggtccccc ggtacggcga
     1201 agaaggatgt tggcggcgct gttgtcgctg acctcgatga tggcggcgca cagctggcgc
     1261 agcggcagcc ggccgcgcgc gcgattggcc gcgaccagcg gcgaattgtc gagcgggtcg
     1321 tccgggccga acggcagctc ctcgtcgagc gaggcctcgc cccgctccac cagcttgagc
     1381 acgaagccgg cgagcggcag cttgaaggtg gaagccatgc cgtagcgcga atcggcgtcg
     1441 aagcgcagct cgcggcccga gccggtgtcg atcgccgcga ccccgaggcg gccgccggga
     1501 cccagagtgg cgcggatcgc ctcgaagcgc tcggcggcat cgccgggcgg cgaagcggcc
     1561 gggacgggcc gggcgcaggc cccggcggcg agcgcgccgg cgaggaaggt gcggcgatcg
     1621 atcatctcat ctcccctgca tgtcgtgcga ttcgcccggc atccggacgg taagctcgtc
     1681 gagcgtctcg tcgagccaga tctggcaggc gagacgcgac gtgcgcgtga cccgggcggc
     1741 gaggtcgagc aggtcctcct cctcctcgcc ggcgcgcggc agtttggcga aatcctcggc
     1801 ggcgacgatg acgtggcagg tcgagcaggc catctggccc tcgcatgttc cttccagcgg
     1861 ctggccgtgc gcctgggcga ggtcgagcag gcgctccccg ggccggccgc gcacctcctg
     1921 gacatggttg ccgtcggcgg agacgaagcg gaccagagtc atgcggcgag gccgggctgg
     1981 cgggaggcgg cggagtcgat cagggcgagc gcctcggcca gctcctcccc ggtcgtgtag
     2041 cggccgaagc cgaggcgcag gctggagcgg gcctcgcggt cggcgaggcc gagggcggcg
     2101 aggacgtggc tcggccggcc cgagccgctg gcgcaggccg agccggccga gaaggcgatg
     2161 ccgcgaagct ccgagatcag ccgggccgcg tcgacgccgt cgcggcgcag gttgagattg
     2221 ccgcgatagc gccggtcgtc cgagccgttg agggtccagc cggccatctg cgccctgccc
     2281 ttcgcccaga ggcgctcgac gtgggcccgg tcctcctcac cgcgctcgac gagcaaacgg
     2341 gcggcctcgc cgaagccgac gcaaagcgcc ggcgacaggg agccggagcg gaggccgcct
     2401 tcctggccgc cgccgtggag cagggggtcg agctcgaccc cgccgcgcac ccagagggcg
     2461 ccgatgccct tggggccgtg gaccttgtgc gccgagacgg cgacgaggtc gcagtcgccg
     2521 agggggaccg gcacccggcc gaagccctgg acggcgtcgc acaggaacaa ggctccggcg
     2581 gcacgggcca tcttaccgat ccggtcgacc ggctggatga cgccgatctc gttgttgacc
     2641 agcatggcgg cgacgaggcc ggtcggcgtg gcgagcgcgg cttcgacggc cgcgaggtcg
     2701 accaggccgt cgggcgcgac ggggaggatt tcggagtcga agcccaggct cgcgag
//
LOCUS       DASVQZ010000415         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_376050, whole genome shotgun sequence.
ACCESSION   DASVQZ010000415 DASVQZ010000000
VERSION     DASVQZ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376050"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..996)
                     /locus_tag="VF547_11710"
     CDS             complement(103..996)
                     /locus_tag="VF547_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263530.1"
                     /translation="MDYEPARVTARPKRPLWSLLALPLIAFVAGVAAMGWLLARSGTA
                     AALLGVAPAPPPAAEPAVPTVAVERAPAAEPQPEAPAGERLVIDPETTRRVNRLEERL
                     ALLDLQSRTAVGNADRAEGLLVAFAARRAIDRGVALGYIETLLRQRFAATEPQAVATV
                     LTVARQPVTVQSLQKQFQDVAPHLSGGGQAQSWWGAFKTEMAGLVTIRRENTPSTQPA
                     ERLRRAAQSLEAGQVEVALLEVLRLPGHERADPWIANARRYVAAHRALDRLESAALLE
                     PRAPAAARPAAPAPAPAPKAR"
     gene            complement(986..1663)
                     /locus_tag="VF547_11715"
     CDS             complement(986..1663)
                     /locus_tag="VF547_11715"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX8263531.1"
                     /translation="MRLLILRPEPGASETAARARALGLDPLVAPLFAIRPLEIGAIEA
                     ERFDAVLITSANGARHAPAGLTGLPCFAVGESSAAAARRAGFADVRVGPSDGAAVAAL
                     MEAAGVARAIHPCGVDHLPVEAGEVAIERLPVYSAEPVERPPFEGPAVAMVHSPRAGA
                     RFAQLAGDRSAFSLAAISAAAAAAAGDGWAAKAVAAAPRDQALLELAAKLCNHGFEGT
                     KDGGDGL"
     gene            complement(1660..>1862)
                     /locus_tag="VF547_11720"
     CDS             complement(1660..>1862)
                     /locus_tag="VF547_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX8263532.1"
                     /translation="CRSPVAALAGLDGDSIHLRAEILTEDGRESERGEARFASGDEDA
                     PLALARSLLDRASPGLRMLFEP"
BASE COUNT          244 a          699 c          690 g          229 t
ORIGIN      
        1 aataaggcct catccatgct ggcctcctcc cagccagcat gttgaatcag aagccagccg
       61 tcttgggaat cccctttcga ttcaacgaag cgcgatcatg ctctagcgcg ccttcggagc
      121 cggggcggga gccggagccg ccgggcgcgc cgccgccggc gcgcgcggct cgagcagggc
      181 ggcgctctcc agccggtcga gcgcgcggtg ggcggcgacg taacggcggg cgttggcgat
      241 ccagggatcg gcgcgctcgt ggccgggcag cctcagcact tcgagcagag ccacctccac
      301 ctgccccgcc tcgaggctct gagcggctcg gcgaagccgc tcggccggct gggtcgaggg
      361 cgtgttctcg cggcggatgg tgaccagccc cgccatctcg gtcttgaacg cgccccacca
      421 gctctgcgcc tggccgccgc ccgaaagatg gggcgcgacg tcctggaact gcttctgcag
      481 gctctgcacc gtcaccggct ggcgggcgac ggtcaggacc gtggccacgg cctgcggctc
      541 ggtggcggcg aagcgctgcc tgagcagggt ctcgatatag cccagggcga ctccgcggtc
      601 gatcgcgcgg cgggcggcga aggcgaccag cagcccttcg gcccggtcgg cattgcccac
      661 ggcggtgcgc gactgcaggt cgagcagcgc gagccgctcc tcgagccggt tgacccggcg
      721 ggtggtttcc gggtcgatca ccagccgttc cccggcgggc gcctcgggct gcggctccgc
      781 ggccggcgcc cgctcgacgg ccaccgtcgg gacggccggt tccgccgcgg ggggaggggc
      841 cggagcgact ccgaggaggg cggcggcggt gccggagcgg gcgagcagcc agcccatcgc
      901 cgcgactccc gcgacgaagg cgatgagcgg cagcgcgagc agcgaccaga gcggtcgctt
      961 cggcctggcc gtcactcgtg ccggctcata atccatcgcc gccgtccttc gtcccttcga
     1021 agccatgatt gcacagcttg gcggcaagct ccagcaaggc ttggtcgcgc ggcgccgccg
     1081 ccaccgcctt ggcggcccag ccgtccccgg ccgctgcggc cgcggccgcg ctgatcgcgg
     1141 cgagggagaa cgcggaccgg tcgccggcga gctgcgcgaa gcgcgcgccc gcccgcggcg
     1201 agtggaccat cgccaccgcc ggcccttcga aaggcggccg ctccaccggc tcggccgaat
     1261 agacgggcag ccgctcgatc gcgacttccc ccgcctcgac cgggagatgg tcgactccgc
     1321 agggatggat cgctcgggcc acgcccgccg cttccatgag agcggcgacg gccgctccgt
     1381 cggaaggccc gacgcgaacg tcggcgaacc cggctcgccg ggctgcggcg gcgctgctct
     1441 cgccgaccgc gaagcagggg agccccgtca ggcccgccgg cgcgtgcctc gccccgttgg
     1501 cgctggtgat cagcaccgcg tcgaagcgct ccgcctcgat ggcgccgatc tcgagcggcc
     1561 tgatagcgaa gagcggagcg acgagcgggt ccagcccaag cgcgcgcgcc ctcgcggcgg
     1621 tctccgaggc gcccggctcc ggacgcagga tgagcagtct cacggttcga acagcatccg
     1681 aagcccgggc gacgcgcggt cgagcagaga ccgcgccagc gccagcggcg cgtcctcgtc
     1741 gccgctcgcg aagcgcgcct cgccgcgctc cgattcgcgg ccgtcctcgg tcaggatctc
     1801 ggcgcgcagg tggatcgagt cgccgtcgag cccggcgagc gccgccaccg gcgagcggca
     1861 gc
//
LOCUS       DASVQZ010000416         3740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_664218, whole genome shotgun sequence.
ACCESSION   DASVQZ010000416 DASVQZ010000000
VERSION     DASVQZ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664218"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /gene="sufB"
                     /locus_tag="VF547_11725"
     CDS             <1..1331
                     /gene="sufB"
                     /locus_tag="VF547_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8263533.1"
                     /translation="GEPEWLLEWRLKAYRAWLEMEEVSWAKLNIPAIDYQDAYYYAEP
                     RQKPTLASLDELDPEIRRTYEKLGIPIEEQKVLAGVEGARKIAVDAVFDSVSVATTFR
                     AELEKAGVIFRSISEAVREYPELVRKYLGSVVPQRDNYFACLNSAVFSDGTFVYIPEG
                     VRCPMELSTYFRINAANTGQFERTLIVADKGSYVSYLEGCTAPMRDENQLHAAVVELV
                     ALDDAEIKYSTVQNWYPGDAEGKGGIYNFVTKRGACRGDRSKISWTQVETGSAITWKY
                     PSCILQGEESVGEFYSVALTNNRQQADTGTKMIHIGKGSRSTIVSKGISAGRSNNTYR
                     GLVRVLANAEGVRNFTQCDSLLLGDQCGAHTVPYIEVRNPTAQIEHEATTSKISDDQL
                     FYAMQRGLSQEDAVALIVNGFAREVLKQLPMEFAVEAQKLLGISLEGSVG"
     gene            1328..1669
                     /locus_tag="VF547_11730"
     CDS             1328..1669
                     /locus_tag="VF547_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263534.1"
                     /translation="MIAALFVALLASQTTGDVFAAMAIDSYVDCLRSKSARPPLERRI
                     ADEQVRLAGLQCRTLREAMVESFIPLVPEALETREARRQEAERGAAAIDSGVAHWLTL
                     KPLVIVPSQPK"
     gene            1677..2486
                     /gene="sufC"
                     /locus_tag="VF547_11735"
     CDS             1677..2486
                     /gene="sufC"
                     /locus_tag="VF547_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX8263535.1"
                     /translation="MLRIDNLHATVADKPILKGLTLAVDAGEVHAIMGPNGAGKSTLA
                     YVLGGRPGYEVTEGSVRFSPNSVRAEPVEARFSSGAEGEEEWVDLLEMAPDERAAAGL
                     FLGFQYPVEIPGVSNVQFLRTALNAQRRARGEAEISAGDFLRLAREQGDALGVSMDMM
                     KRNVNVGFSGGEKKRNEMVKMGIVDPRLAVLDETDSGLDIDALRVVSDGINRIMRKPD
                     KAVLLLTHYQRLLDLVVPDFVHVLAEGRIVRSGSPELARQLEREGYAEMAA"
     gene            2638..>3740
                     /locus_tag="VF547_11740"
     CDS             2638..>3740
                     /locus_tag="VF547_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX8263536.1"
                     /translation="MLDLPSPKAEAWRWSDLSALPDIAARPSSGTVPEALPWIECEHE
                     GARLLFVDGILAAHRSRLGPVEIGAVDRHEEDHALARLVGREGWVLRLGRDHAPAGLI
                     QIVHVSTGAADHLPARIALHDDAQASIVETFVGDGWINRLASITLGRSARLMLSRRLI
                     GSGGFVSLADRAVVGEGASLILTTLAAGGADSRLDGAVLLEGQGAFAEAGGALLARGR
                     QRHDANFVLRHGAPGGTSRQVWRSVADDRAICSVAARVEVARGAQKTDGEQSLKGIML
                     SRSATINAKPELEIFADDVKCAHGATVGELDRDALFYLASRGVPEAEAKALLTRAFVA
                     DSIDRTGEEGVREAMHADVESWFAKVPLPPGER"
BASE COUNT          618 a         1343 c         1218 g          561 t
ORIGIN      
        1 agggcgagcc cgagtggctg ctcgagtggc ggctgaaggc ctatcgggcg tggctggaga
       61 tggaggaagt gagctgggcc aagctcaaca tccccgccat cgattaccag gatgcttatt
      121 attacgccga gccgcgccag aagccgaccc tcgcctcgct ggacgagctc gacccggaga
      181 tccgccggac ctacgagaag ctcggcatcc cgatcgagga gcagaaggtg ctcgccgggg
      241 tcgagggggc gcgcaagatc gccgtcgacg cggtgttcga ctccgtctcg gtcgccacca
      301 ccttccgggc cgagctcgag aaagcgggcg tgatcttccg ctcgatctcc gaggcggtgc
      361 gcgaatatcc ggagctggtc cgcaaatatc tcggctcggt ggtcccgcag cgcgacaatt
      421 acttcgcctg cctgaacagc gccgtcttct ccgacggcac cttcgtctac atccccgaag
      481 gcgtgcgctg cccgatggag ctgagcacct atttccggat caacgccgcg aacaccggcc
      541 agttcgagcg caccctgatc gtcgccgaca agggcagcta cgtctcctat ctcgaaggct
      601 gcaccgcgcc gatgcgcgac gagaaccagc tccacgccgc cgtcgtcgag ctggtcgcgc
      661 tggacgacgc cgagatcaag tacagcaccg tccagaactg gtatcccggg gacgccgagg
      721 gcaagggcgg catctacaat ttcgtcacca agcgcggcgc ctgccgcggc gaccgctcca
      781 agatcagctg gacccaggtc gagacggggt cggcgatcac ctggaaatac ccgtcctgca
      841 tcctccaggg cgaggagagc gtcggcgaat tctattcggt cgcgctgacc aacaaccgcc
      901 agcaggccga caccggcacc aagatgatcc acatcggcaa gggcagccgc tcgaccatcg
      961 tctccaaggg catcagcgcc ggccgctcga acaacaccta tcgcggcctc gtccgcgtcc
     1021 tcgccaatgc cgagggcgtg cgcaacttca cccagtgcga ttcgctcctg ctcggcgacc
     1081 agtgcggcgc ccacaccgtc ccctatatcg aggtccgcaa cccgaccgcc cagatcgagc
     1141 acgaggcgac gacctcgaag atcagcgacg accagctctt ctacgccatg cagcgcggcc
     1201 tcagccagga ggacgccgtc gccctcatcg tcaacggctt cgcccgcgag gtcctcaagc
     1261 agctgccgat ggagttcgcc gtcgaggctc agaagctgct ggggatcagc cttgagggga
     1321 gcgtgggatg atcgcggcgc tgttcgtcgc cctgctcgcc tcgcaaacga ccggggatgt
     1381 ctttgcggcc atggccattg attcctacgt ggactgtctg cgcagcaagt ccgcgcgtcc
     1441 ccccctcgag cggcgcatcg ccgatgaaca agtgcgtttg gccggcctgc aatgccggac
     1501 gctgcgcgaa gcgatggtcg aatccttcat tccactggtg cccgaagcgc tggagacgcg
     1561 agaggcgagg cggcaggagg cggaaagggg cgctgccgca atcgattccg gcgtcgctca
     1621 ttggctgacg ctcaagccgc tggtcatcgt gccaagccaa ccgaaatgaa ctgataatgc
     1681 tcagaatcga caatctccac gccaccgtcg ccgacaagcc gatcctcaag ggcctgaccc
     1741 tcgcggtgga cgcgggcgag gtccacgcca tcatgggtcc caacggcgcc ggcaagtcga
     1801 ctctcgccta cgtgctcggc ggacggcccg gctatgaggt gacggaaggc tctgtgcggt
     1861 tcagccccaa ttccgttcgg gctgagcctg tcgaagcccg cttctcttcc ggggcggaag
     1921 gggaagagga gtgggtcgac ctgctcgaga tggcccccga cgagcgcgcc gccgccggcc
     1981 tgttcctcgg cttccagtac ccggtcgaga tacccggcgt ctcgaacgtc cagttcctcc
     2041 gcaccgcgct caacgcccag cgccgggcgc gcggcgaggc ggagatttcc gcgggcgact
     2101 tccttcggct ggcgcgcgag cagggcgacg ctctcggcgt ctcgatggac atgatgaagc
     2161 gcaacgtgaa cgtcggcttc tcgggcggcg agaagaagcg caacgagatg gtcaagatgg
     2221 gcatcgtcga cccgcgcctg gcggtgctcg acgagaccga ttccggcctc gacatcgacg
     2281 ccctaagggt ggtctccgac ggcatcaacc ggatcatgcg caagcccgac aaggcggtgc
     2341 tcctcctcac ccactaccag cgcctgctcg acctcgtcgt gcccgacttc gtccacgtcc
     2401 tcgccgaagg ccggatcgtc cgctcgggca gccccgagct cgcccgccag ctcgagcgcg
     2461 aaggctatgc cgagatggcg gcatgaccgg cgcgcttcaa ttcctccccg ggacggggag
     2521 ggggaccatg cgaagcatgg tggagggggc ccgccgcccc ccctcgtcga tgctgggccc
     2581 cctccacgac gccgcttcgc gccgcggtcc tccgccccgt tccggtgagg cgctatgatg
     2641 ctcgatctcc cctcgcccaa ggccgaagcc tggcgctgga gcgacctgtc ggcgcttccg
     2701 gacatcgccg cccgcccgtc ctcggggacg gtcccggagg cgctgccctg gatcgaatgc
     2761 gagcatgaag gcgcccgtct gctcttcgtc gacggaatcc tcgcggcgca ccgaagccgc
     2821 ctcgggccgg tcgagatcgg cgccgtcgac cgccacgagg aggatcacgc gctcgcccgg
     2881 ttggtcggcc gcgaaggctg ggtgcttcgg ctgggccgcg accatgcgcc ggccggcctc
     2941 atccagatcg tccacgtctc gaccggcgcg gccgaccatc tcccggcccg gatcgccctc
     3001 catgacgatg cccaggcctc gatcgtcgag accttcgtcg gcgacggctg gatcaaccgg
     3061 ctggccagca tcaccctggg ccggagcgcc cgcctgatgc tgtcgcggcg gctgatcggc
     3121 agcggcggct tcgtcagcct ggccgaccga gccgtcgtcg gcgagggggc aagcctgatc
     3181 ctcacgaccc tcgccgccgg cggcgccgac agccgcctcg acggcgccgt cctgctcgag
     3241 ggccaaggcg ccttcgccga ggcggggggc gccctgctgg cccgcggccg ccagcgtcat
     3301 gacgccaatt tcgtccttcg ccacggagcg cccggcggca ccagccggca ggtctggcgc
     3361 tcggtcgccg acgaccgcgc gatctgctcg gtcgccgccc gggtcgaggt cgcccgcggc
     3421 gcccagaaga ccgacggcga gcagtccttg aagggcatca tgctcagccg ctcggcgacg
     3481 atcaacgcca agcccgagct cgagatcttc gccgacgacg tcaaatgcgc ccacggcgcc
     3541 accgtcggcg agctcgaccg agatgccctc ttctacctgg ccagccgcgg cgttcccgag
     3601 gccgaggcca aggccctcct cacccgagcc ttcgtcgccg actcgatcga ccgcaccggc
     3661 gaggaggggg tgcgcgaggc gatgcacgcg gacgtcgaaa gctggttcgc aaaagtccct
     3721 ctccctccag gggagaggga
//
LOCUS       DASVQZ010000417         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_282364, whole genome shotgun sequence.
ACCESSION   DASVQZ010000417 DASVQZ010000000
VERSION     DASVQZ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282364"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1036
                     /gene="fghA"
                     /locus_tag="VF547_11745"
     CDS             197..1036
                     /gene="fghA"
                     /locus_tag="VF547_11745"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX8263537.1"
                     /translation="MTLETLTSARSFGGTQLALRHPSRETGTDMTFGLYLPPQAGAGG
                     PLPVLWFLSGLTCTHANVVEKGQYQRVCAELGLILVAPDTSPRGPDVPDDSAYDFGQG
                     AGFYVDSTQEPFAKNYRMESYVAAELPDLLASHFPADMSRQGITGHSMGGHGALTLAL
                     RHRARYRSVSAFAPIVAPSQVPWGEKGLGGYLGPDRSQWRAHDAVALIEDGARLPELL
                     VDQGTVDSFLERELRPELLEAACRDAGIPLTLRMQPGYDHSYFFISTFIEDHLRWHRE
                     RLG"
     gene            1134..1913
                     /gene="cysQ"
                     /locus_tag="VF547_11750"
     CDS             1134..1913
                     /gene="cysQ"
                     /locus_tag="VF547_11750"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8263538.1"
                     /translation="MTVPDLLPTLIEAALAAGEEIERIYAEGCETEEKEDGSPVTIAD
                     RHAEAIILERLGAAFPGIPVLAEEEAAAGRIPDLGRRFFCVDPLDGTRGFVQRTGEFT
                     VNIGLIEDGAPLAGVIYAPDPKLLYFGARGAGAFRGRGGGVPEPIAPRPRPASGLTAV
                     GSRSHAAHGTAAKTAHLGIAEFVASSSSLKFCLVAEGTADVYPRHGTTAEWDTAAGQA
                     ILEAAGGRVMALDEEGRETGPLAYGKIEARFLNPPFIAWGS"
     gene            complement(2014..2430)
                     /locus_tag="VF547_11755"
     CDS             complement(2014..2430)
                     /locus_tag="VF547_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8263539.1"
                     /translation="MTVQETYAQPLEAIPEDEATDFAHELISSRRVEGTAVYSEAGEK
                     LGTIHSVMIGKKSGQVAYAVLSTGGWLGIGAHVHPLPWALLTYDVDRDGYVVSIGRDQ
                     LAKAPSMALDETDRPISRDDQERITSYWGTLPWWGL"
BASE COUNT          397 a         1038 c          886 g          404 t
ORIGIN      
        1 cgacatcaac caggcgttcg acctgatgca cgccggcgag agcatccgga gcgtggtcgt
       61 gtactgacgt ccttcgagac gggccttcgc caggctcagt ccctcctcag gatgagcggg
      121 agggatcgca gtcatccagc accgctcatc ctgagcagcc ggcgagcttg tcgaaccggc
      181 gaaccgaagg accgccgtga ccctcgagac cctcacctcc gcaagatcct tcggcggcac
      241 ccagctcgcc ctgcgccacc ccagccgcga gaccggcacc gacatgacgt tcggcctcta
      301 cctccccccg caggccgggg cgggcgggcc cctccccgtc ctctggttcc tgtcgggcct
      361 gacctgcacc cacgccaacg tggtcgagaa gggacagtac cagcgcgtct gcgccgagct
      421 cggactgatc ctcgtcgccc cggacaccag cccgcgcggc cccgacgtcc ccgacgactc
      481 cgcctacgat ttcggccagg gcgccggctt ctacgtcgat tcgacgcagg agcccttcgc
      541 gaagaactac cgcatggaaa gctacgtcgc cgcggagctt cccgatctcc tcgcctcgca
      601 tttcccggcg gacatgtccc gccaggggat caccggccat tcgatgggcg ggcatggcgc
      661 cctcaccctc gcgctgcgcc atcgagcccg ctaccgcagc gtctccgcct tcgcgccgat
      721 cgtcgccccg tcgcaggtgc cctggggcga gaagggcctg ggcggctatc tcggccccga
      781 ccgctcgcaa tggcgggccc atgacgccgt cgccctgatc gaggacggcg cgcggctgcc
      841 cgagctgctg gtcgaccagg gaaccgtcga cagcttcctc gagcgcgagc ttcggcccga
      901 gcttttggaa gccgcctgcc gcgacgccgg catcccgctg acgctcagga tgcagcccgg
      961 ctacgaccac agctatttct tcatctcgac cttcatcgag gaccatctgc gctggcaccg
     1021 ggagcggctt ggctagccga cctgccggcg aaggctggca tcccggcgag aataggctac
     1081 cggagccctt cacccctggg ggccccggcc ttcgccgggc cagcgagcat gcgatgaccg
     1141 ttcccgacct cctccccacg ctgatcgaag ccgcgctcgc cgccggcgag gagatcgagc
     1201 ggatctacgc cgaaggctgc gagacggagg agaaggagga cggctcgccc gtcaccatcg
     1261 ccgaccgcca tgccgaggcg atcatcctcg agcggctcgg cgccgccttc cccggcatcc
     1321 ccgtcctcgc cgaggaggag gccgccgccg ggcggatccc cgacctcggc cggcgcttct
     1381 tctgcgtcga cccgctcgac ggcacccgcg gcttcgtcca gcgcaccggc gagttcacgg
     1441 tcaacatcgg cctgatcgag gacggcgcgc cgcttgccgg ggtgatctac gcgcccgatc
     1501 cgaagctgct ctatttcgga gcccgcggcg ccggcgcctt ccgcggccgc ggcggcggcg
     1561 tgcccgagcc gatcgccccg cgcccccgcc cggcctccgg cctgaccgca gtcggcagcc
     1621 gaagccacgc ggcgcacggc acggcggcga agaccgcgca tctcggcata gccgagttcg
     1681 tcgcctcgag ctcgtcgctc aaattctgcc tggtcgcgga agggacggcc gacgtctatc
     1741 cgcgccacgg caccacggcc gaatgggaca ccgccgccgg ccaggcgatc ctcgaggcgg
     1801 ccggggggcg ggtgatggcg ctcgacgagg agggccgcga gaccggcccc ctcgcctacg
     1861 gcaagatcga agcgcgcttc ctcaaccccc ccttcatcgc ctgggggtcg tgaccgtcat
     1921 tgcgaggagc gtagcggcgc ggcactccag cgacggagcc cgcgcgggcc ggcgtctgtg
     1981 gattgcttcg cttcgttcgc aatgacgggc gggtcacagc ccccaccagg gcaaggtgcc
     2041 ccaataggag gtgatccgct cctgatcgtc gcggctgatc ggccggtcgg tctcgtcgag
     2101 cgccatcgag ggcgccttgg cgagctggtc gcggccgatc gagacgacat agccgtcgcg
     2161 gtcgacgtcg taggtcagca atgcccaggg cagcgggtgg acatgggcgc cgatcccgag
     2221 ccacccgccc gtcgacagca ccgcataggc cacctggccg ctcttctttc cgatcatcac
     2281 gctgtggatg gtgccgagct tctcgccggc ctcgctatag acggcagtgc cctcgacccg
     2341 ccgcgacgag atcagctcgt gcgcgaaatc cgtcgcctcg tcctccggga tcgcttcgag
     2401 gggctgcgca taggtctcct ggaccgtcat cgttcgtctc ctcgtccgcc ggccgcgaga
     2461 agaacggggg cgccccagcc gaagccggga cgcccccata tccgccctcg cgggcggctg
     2521 ttgcgctttt tcgaccgatg ccgatggaac gaacggccgc ctcgcgggtt ccggctcact
     2581 ccgccgagcc ggttccgtct gctgccgcgc ttttggcccg gcgcaggcgc aacgcctgag
     2641 caacgttccg gcgcggccta gccgaagccg tgcttgatcc gcgctgccgt cattgcgagg
     2701 agcgcagcga cgaagcaatc cagcc
//
LOCUS       DASVQZ010000418         7759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_185829, whole genome shotgun sequence.
ACCESSION   DASVQZ010000418 DASVQZ010000000
VERSION     DASVQZ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185829"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF547_11760"
     CDS             <1..474
                     /locus_tag="VF547_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8263540.1"
                     /translation="AVLLLLYGSGLRVAEALGLTGAALPLGEALSVTGKRSKTRIVPL
                     LAPVRAAIERYLALCPYAAERDEPIFRGARGGPLNGEMIRRVVRGARARLGLSDRTTP
                     HALRHSFATHLLGRGADLRSLQELLGHASLSSTQIYTAVDAAHLMDVYRNAHPRA"
     gene            complement(507..1109)
                     /locus_tag="VF547_11765"
     CDS             complement(507..1109)
                     /locus_tag="VF547_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8263541.1"
                     /translation="MGDWTIRLIDQTGYVGIFLLMFLETIFPPIPSEVIMPIAGVRAA
                     QGPLTLWGVIASGTAGAMLGNLFWYALARVIGLRRFRPFIERYGRWLTMDWDDVEKAE
                     RMFGRFGGLIVSLGRMLPTIRSVISIPAGLLSMRLKSFLIWSTLGTVIWTAALATAGW
                     GLGKAFRDIEKVLGPLSTAVIVAIVAAYIWRQLTWRRRHP"
     gene            complement(1209..2159)
                     /gene="gshB"
                     /locus_tag="VF547_11770"
     CDS             complement(1209..2159)
                     /gene="gshB"
                     /locus_tag="VF547_11770"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX8263542.1"
                     /translation="MTLRVAVQMDPLEGINIEGDSTFAIMLGAQKRGHTLHYYRAEDL
                     SYADGRLWTMARRVTVRPVPGDHFTLGEHSLLDLGRDTDVVLMRQDPPFDLGYITATH
                     LLERIQGETLVVNDPASVRNAPEKVWVLDFARYMPPTLLTRSLGAARKFLEEHGEIVV
                     KPLYGNAGKAVFKVGRDGANLASLIEMFNTAYREPHVVQAFVPEIAEGDKRIVLVDGE
                     VAGAINRIPGAGEIRSNLAVGGSAAKTDLTATEREICAALAPELKARGLLFVGIDVIG
                     GKWLTEINVTSPTGIVVIDRFNGTDTPALIWDSIEAKVRR"
     gene            complement(2332..2682)
                     /locus_tag="VF547_11775"
     CDS             complement(2332..2682)
                     /locus_tag="VF547_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX8263543.1"
                     /translation="MTRQRAERGGRRAERLAAFWLQLKGWKILGRRVRTPVGEVDLIA
                     RRGRTVAFVEVKARSTLEEAGFSLDEYRLRRVAAAAEALAHRFAREGDDIRLDAIFMV
                     PRRLPRHMVNIWHG"
     gene            complement(2679..3521)
                     /gene="rsmI"
                     /locus_tag="VF547_11780"
     CDS             complement(2679..3521)
                     /gene="rsmI"
                     /locus_tag="VF547_11780"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX8263544.1"
                     /translation="MTLILMNALPPGLYIVATPIGNLSDLSPRAADVLAKADLIAVED
                     SRVTAKLLRHIGVKRPMLPYHDHNAERVRPQLLERMRDGAVALVSDAGTPLISDPGYK
                     LVRDARAAGIGVTTIPGPSAVVAALTLAGLPTDRFLFVGFLAAKAGARATAIAEIASI
                     RATLVLYESGPRLAATLEALKAGLGEREAAVAREISKKFEETVTGSLAELAARYAEAA
                     PKGEIVIVVAPPGEAEAVGEDEVDEALREAMDRLSPSRAAAEVAERLGVPRRQAYERA
                     LKLK"
     gene            3520..4713
                     /locus_tag="VF547_11785"
     CDS             3520..4713
                     /locus_tag="VF547_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX8263545.1"
                     /translation="MAEHSPGSQARRYFLGLGAAMGLALGGCQLVPKPRPEAPPEPEL
                     PEAPPEPEEAPGPQLPPGETRNRVAVLVPTSGANAGVGQSIANAANLALLDAGNDRIR
                     ITIYDTARGGAAAAANQALAEGNGLFLGPLLAEDVRAVAPVARRAGVPVISFSNDTSV
                     AGNGVYLMGFTPAQSIARVVDHARGQGLDRFGAVTPEGIYGRRAAQAMIDSVERSGGR
                     LVAMQSYAGGPGGLRTAVGKLQALGGYDAVLIADGGRTAAAAAPLLKAGSAGIRLLGT
                     ELWATETGLGRTPALRGAWFASVSDGMFNQLRTRYRARYGANPYRLASLGYDSVLLTV
                     RIAKNWRLGRPFPERELRDPVGFAGVDGPFRFTPNGIAERSLEVREVTATGTITVSPA
                     PRSFD"
     gene            complement(4739..5869)
                     /gene="hemW"
                     /locus_tag="VF547_11790"
     CDS             complement(4739..5869)
                     /gene="hemW"
                     /locus_tag="VF547_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX8263546.1"
                     /translation="MLRCARNDGLALYVHWPFCVSKCPYCDFNSHVRESVDQAAWREA
                     LLRDLAWEAGVYPGRPLSSIFFGGGTPSLMEPATVAALIEAAGRHWGLAPDVEITLEA
                     NPSSVEAARFADLAAAGVNRVSLGLQALDDPALRFLGRAHDSAEGLAALATAQACFDR
                     VSFDLIYALPGQTEAAWAAELGRALGFGTGHASLYQLTIEPGTRFAAMAARGELAAQD
                     PDEAAALFELTQEMTRAAGLPAYEISNHARPGEESRHNLAYWRYRPYLGIGPGAHGRR
                     SGLATLRHRKPENWLAALARNGHGLAEETPLSREEQATEALLMGLRLSAGVELEAVEP
                     ALDFEAVERLEAQGLLTRSSDRLVVPANGRLLLDAILAEIAL"
     gene            complement(5938..6585)
                     /gene="rdgB"
                     /locus_tag="VF547_11795"
     CDS             complement(5938..6585)
                     /gene="rdgB"
                     /locus_tag="VF547_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX8263547.1"
                     /translation="MRKLQPGRLVIASHNVGKVREIKDLLGPYGIEPVSAAELDLPEP
                     DEIGVNFVENADLKAREAADLSGLPALADDSGLCVDALEGRPGIFSARWALAADPRVA
                     PANGPGEVEGPRDFDLAMRRVWEELEALGPEASRNAHFVCALAVCWPDGRSEWFEGRV
                     DGTLVWPPRGDRGFGYDPMFVPAGRDLTFGEMDPAEKHRISHRADAFRKLVAALL"
     gene            complement(6635..6997)
                     /locus_tag="VF547_11800"
     CDS             complement(6635..6997)
                     /locus_tag="VF547_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263548.1"
                     /translation="MALRPEQVSDPEWRAWLAAAPWPLQPDESDNLPKAVLSWLRQAP
                     STELLRRHEADELIDDFLLYSRHLHRAQRADVSNSGFVFVGAVVGLIGYAVGGPVGAL
                     AGPLFNFILAGPPPPASK"
     gene            complement(6969..7358)
                     /locus_tag="VF547_11805"
     CDS             complement(6969..7358)
                     /locus_tag="VF547_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263549.1"
                     /translation="MNVRSSAQEMRNQLETSREQLEAIQASDRHLVLTSTASITAICV
                     VASMELLQFLALVRPPIASPLDTFPVLKVAAGAIALLCVAYIAFSLYRRRRDVVRNDI
                     RALRQQIDILVARGAWTSDGPASGASL"
     gene            complement(7392..>7759)
                     /locus_tag="VF547_11810"
     CDS             complement(7392..>7759)
                     /locus_tag="VF547_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX8263550.1"
                     /translation="IQADGGTRTAAISGAWVALRIAVDRLIEQQLLAADPIRQKVAAV
                     SCGIHGGAAVLDLDYEEDSTAGADGNFVLTADGSIVEAQVTAEGECYDEEGLLRLLRL
                     ARIGCGEIFAAQDKATGRL"
BASE COUNT         1160 a         2734 c         2688 g         1177 t
ORIGIN      
        1 gccgtgctgc tcctgctcta cggctccggc ctgcgcgtgg cggaggcgct cggcctcacc
       61 ggcgccgcct tgccgctggg cgaggcgctc agcgtaaccg gcaagcggtc gaagacccgg
      121 atcgtgccgc tgctggcgcc ggtccgggcc gcgatcgagc gctatctggc gctgtgcccc
      181 tatgcggccg agcgcgacga gccgatcttc cggggcgccc ggggcgggcc cctcaatggc
      241 gagatgatcc gccgtgtcgt gcgcggcgcc cgagcccgtc tcggcctttc cgaccggacg
      301 acgccgcatg cgctccgcca cagcttcgcg acccacctgc tcggccgggg ggcggacctg
      361 cgctcgcttc aggagctgct cggccacgcc agcctgtcgt cgacccagat ctacaccgcg
      421 gtcgacgccg cgcatttgat ggacgtctac cgaaacgccc atccgcgggc ctgatcatcc
      481 tccccatcgc gatgcgacgg gcaggatcag gggtggcgcc tgcgccaggt gagctggcgc
      541 cagatgtagg cggcgacgat cgccacgatc accgccgtcg acagcggtcc gagcaccttc
      601 tcgatgtcgc ggaacgcctt gcccagcccc cagccggccg tcgccagcgc ggccgtccag
      661 atcaccgtgc cgagcgtcga ccagatcagg aagctcttca gccgcatgct gagcagcccc
      721 gccgggatcg agatcaccga gcggatcgtc ggcagcatcc gcccgagcga gacgatgagg
      781 ccgccgaagc gcccgaacat ccgctccgcc ttctcgacat cgtcccagtc catggtcagc
      841 cagcggccgt atctctcgat gaacggccgg aagcgtctca gcccgatcac ccgggcgagc
      901 gcataccaga acaggttgcc gagcatcgcc ccggcggtgc cgctggcgat caccccccac
      961 aaggtgagcg gcccctgggc cgcgcgcact ccggcgatcg gcatgatcac ctccgacggg
     1021 atcggcggga agatcgtctc gaggaacatc agcaggaaga tgccgacata acccgtctgg
     1081 tcgatcagtc ggatggtcca gtcacccatg gatcgttccc cggcggaggc cggggtcaag
     1141 cggcggcggg cgtaaatctg ggccccggac ttcgccgggg agcggaagcc tcacggcgtg
     1201 ccggtgcctc atcttcgcac cttcgcttcg atcgagtccc agatgagggc gggcgtgtcg
     1261 gtgccgttga accggtcgat gacgacgatc ccggtcggcg acgtgacgtt gatctcggtc
     1321 agccatttgc cgccgatgac gtcgattccg acgaagagca ggccgcgcgc cttgagctcg
     1381 ggcgccagag cggcgcagat ctcgcgctcc gtcgcggtga ggtcggtctt ggccgccgag
     1441 ccgccgaccg ccaggttcga gcgtatctcg ccggcgccgg ggatccggtt gatcgccccg
     1501 gccacctcgc cgtcgacgag gacgatgcgc ttgtcgccct cggcgatctc ggggacgaag
     1561 gcctggacga cgtgcggctc gcgataggcg gtgttgaaca tctcgatcag cgaggcgagg
     1621 ttggcgccgt cgcggccgac cttgaacacc gccttgccgg cattgccgta gagcggcttg
     1681 acgacgatct cgccatgctc ctcgaggaac ttgcgggccg cgccgagcga gcgggtcagc
     1741 agggtcggcg gcatgtaccg ggcgaaatcg agcacccaga ccttctcggg cgcattgcga
     1801 accgaggcgg ggtcgttgac caccagagtc tcgccctgga ttcgctcgag caggtgggtc
     1861 gccgtgatgt agccgaggtc gaagggcgga tcctggcgca tcaggacgac gtcggtgtcg
     1921 cggccgaggt cgagaagact gtgctcgccg agcgtgaaat gatcgcccgg caccggccgc
     1981 acggtgaccc gccgggccat cgtccagagg cgcccgtcgg cgtagctgag gtcctcggct
     2041 cggtaataat ggagcgtgtg gccgcgcttc tgagcgccga gcatgatcgc gaaggtcgaa
     2101 tcgccctcga tgttgatccc ttcgagggga tccatctgaa cggcgaccct gagcgtcatt
     2161 cgattctcct gcgcatcgag gggtccgctc atcctgagta gggactgagc gaaggcgaag
     2221 gcccgtatcg aaggaccgtc cttcgatacg ccgcttcgac ttcgctcagc ggctactcag
     2281 gatgagcggg gctgcggcgc gctcggcgtc tagtggacgt cgcggtcttt gtcatccatg
     2341 ccagatgttg accatgtgcc gggggaggcg gcgcggtacc atgaagatcg cgtcgaggcg
     2401 gatgtcgtcg ccctcgcggg cgaagcggtg ggcgagcgct tccgcggcgg ccgcgacccg
     2461 ccgcaggcgg tactcgtcga gcgagaagcc cgcttcctcg agggtcgacc tcgccttgac
     2521 ctcgacgaac gccaccgtcc ggccccggcg ggcgatcagg tcgacctcgc cgaccggcgt
     2581 ccgcactctc cgcccgagga tcttccagcc cttgagctga agccagaagg ccgccagccg
     2641 ctccgcccgt cggccgcccc gctccgcccg ctgccgggtc atttgagctt cagagcgcgt
     2701 tcgtaggcct ggcgccgggg cactccgagc ctttccgcca cctcggcggc ggcgcgggac
     2761 ggggacagcc ggtccatcgc ctcgcgcagc gcttcgtcga cctcatcctc gccgaccgct
     2821 tccgcctctc cggggggcgc gacgacgatc acgatctccc ccttcggcgc cgcctcggcg
     2881 tagcgcgccg cgagctcggc gagtgatccg gtcaccgtct cctcgaactt cttgctgatc
     2941 tcgcgggcca ccgccgcctc gcgctcgccc agcccggcct tcagagcctc cagcgtcgcc
     3001 gcgagccgag gcccgctctc gtagaggacc agggtcgccc tgatcgaggc gatctcggcg
     3061 atcgcggtcg ccctggcgcc cgcctttgcg gccaggaagc cgacgaacag gaagcggtcg
     3121 gtcggcagcc cggccagggt cagcgccgca acgaccgccg acgggcccgg aattgtggtc
     3181 acgcctatcc ccgccgcgcg ggcgtcgcgg accagcttgt agccggggtc cgagatcagc
     3241 ggcgtgccgg cgtccgacac cagggccacc gcgccgtcgc gcatccgttc gagcagctgc
     3301 gggcgaaccc gctcggcgtt gtggtcgtga tagggaagca tcggccgctt gacgccgatg
     3361 tggcgcagca gcttggcggt gacccggctg tcctcgacgg cgatcaggtc ggccttggcg
     3421 aggacgtccg ccgcccgcgg cgacagatcg gaaagattgc cgatgggggt ggcgacgata
     3481 tagaggccgg ggggcagcgc gttcatgagg atgagggtca tggcagagca ttcccccggg
     3541 tcgcaagcgc ggcgctattt cctcggcctc ggagcggcga tgggcctggc cctgggcggc
     3601 tgccagctgg tgcccaagcc ccgtcccgaa gcgccgccgg agccggagct gcccgaagct
     3661 cctccggagc cggaagaggc gcccgggccc cagctcccgc cgggggagac ccgcaaccgg
     3721 gtcgccgtcc tggtgccgac ctccggcgcc aacgccgggg tcggccagtc gatcgccaac
     3781 gccgccaatc tcgctttgct cgacgccggc aacgaccgca tccgaatcac catatacgac
     3841 accgcccgcg gcggcgccgc ggcggccgcc aaccaggcgc tggcggaggg caacggcctg
     3901 ttcctcgggc cgcttctcgc cgaggacgtc cgcgccgtcg cgccggtcgc ccgccgcgcc
     3961 ggggtgccgg tgatcagctt ctccaacgac accagcgtcg ccggcaacgg cgtctacctg
     4021 atgggcttca cgcccgccca gtcgatcgcg cgggtcgtcg atcatgcgcg cgggcagggc
     4081 ctcgatcgct tcggggcggt gactcccgaa ggcatttacg gccgccgagc ggcgcaggcg
     4141 atgatcgaca gcgtcgagcg ttcgggcggg cggctcgtgg cgatgcagag ctatgccggc
     4201 gggcccggag gcctgaggac ggcggtcggc aagcttcagg ccctcggcgg ctacgatgcg
     4261 gtgctgatcg ccgacggcgg gcggaccgcc gccgccgccg cgccgctgct caaggccggc
     4321 tcggcgggga ttcggctgct gggcaccgaa ttgtgggcga ccgagaccgg cctcggccgg
     4381 actccggcgc tgcgcggagc ctggtttgcg agcgtctccg acggcatgtt caaccagctt
     4441 cgcacccggt accgcgcccg ctacggcgcc aatccgtacc ggctggcgag cctgggctac
     4501 gattcagtgc tcctcaccgt tcgcatcgcc aagaactggc gcctcggccg tcccttcccg
     4561 gagcgcgagc tgcgcgatcc ggtcggcttc gccggcgtcg acggcccgtt ccggttcacc
     4621 ccgaacggaa tcgccgagcg atcgctcgag gtgcgcgagg tcaccgccac cggcaccatc
     4681 accgtctcgc cggcgccgcg aagcttcgac taaacgctct ccctgcaggg gagagggtct
     4741 acagcgcgat ctcggcgagg atcgcgtcga ggagcaggcg gccgttcgcc ggcacgacca
     4801 gccggtcgct cgacctcgtc agcaggccct gcgcttcgag ccgttccacc gcttcgaagt
     4861 cgagcgccgg ctcgaccgct tccagctcta ccccggcgct cagcctgagc cccatcagca
     4921 gagcctcggt cgcctgttcc tcccggctca gcggggtctc ctccgcgagg ccgtggccgt
     4981 tgcgggccag cgcggccagc cagttctccg gcttgcggtg ccggagcgtg gccaggccgg
     5041 agcggcggcc gtgtgcgccg gggccgattc cgagataggg ccggtagcgc cagtaagcga
     5101 gattgtgccg gctttcctcg ccgggccggg cgtggttgga gatttcgtag gcgggaaggc
     5161 cggccgcccg cgtcatctcc tgggtcagct cgaagagggc cgccgcctcg tccggatcct
     5221 gggccgcgag ctcgccgcgg gccgccatcg cggcgaagcg cgtccccggc tcgatcgtca
     5281 gctggtagag cgaggcatgg ccggtgccga agccgagcgc ccggccgagc tcggccgccc
     5341 aggccgcctc ggtctggccg ggcagggcgt agatgaggtc gaagctgacc cggtcgaagc
     5401 atgcttgcgc cgtcgccagc gcggccagcc cttccgccga gtcgtgcgcc cggccgagga
     5461 agcggagcgc tgggtcgtcg agcgcctgga ggccgagcga gactcggttc actccggccg
     5521 ccgcgaggtc ggcgaagcgc gccgcctcca ccgaggaggg attggcctcg agcgtgatct
     5581 cgacgtcggg cgcgaggccc cagtgccgtc ccgcggcctc gatcagcgcc gcgacggtgg
     5641 ccggctccat cagcgaaggc gtccccccgc cgaagaagat cgagctgagc ggccggccgg
     5701 gatagacgcc ggcctcccag gccaggtcgc gaagcagggc ctcccgccac gccgcctggt
     5761 cgacggactc gcggacatgg ctgttgaagt cgcaataggg gcatttcgag acgcagaacg
     5821 gccagtggac gtagagcgcg aggccgtcat tgcgagcgca gcgaagcaat ccagattcgg
     5881 cgcccgggcc tctggattgc ttcgtcgctg cgctcctcgc aatgacgccc tcggcgatca
     5941 cagcaaagcc gccaccagct tgcggaaggc gtcggcgcga tggctgatcc ggtgcttttc
     6001 ggcgggatcc atctcgccga aggtgaggtc gcgcccggcg ggcacgaaca tcggatcgta
     6061 gccgaagccg cggtctcccc gcggcggcca gacgagcgtg ccgtccaccc ggccctcgaa
     6121 ccattcgctc cttccgtcgg gccagcatac cgcgagggcg cagacgaaat gggcgttgcg
     6181 gctcgcctcc ggcccgagcg cctccagctc ttcccagacc cgccgcatgg cgaggtcgaa
     6241 gtcgcgcggc ccttcaacct cccccggtcc gttggccggc gcgacccggg gatccgcggc
     6301 gagagcccag cgcgcagaga agatgccggg ccgcccttcc agcgcgtcga cgcaaaggcc
     6361 gctgtcgtcg gcgagagccg gaaggccgga aaggtcggcc gcctcgcgcg ccttcaagtc
     6421 ggcgttctcg acgaagttga cgcctatctc gtccggctcc ggcagatcca gctccgccgc
     6481 cgagaccggc tcgatgccgt aggggccgag caggtccttg atctcgcgga ccttgccgac
     6541 gttgtggctg gcgatgacca gccggccggg ctgaagcttc ctcatgcgcg aattcgatca
     6601 atcaaggcgt ctatcatcat gtccacttcg tcgatcattt gcttgccggc ggcggtggtc
     6661 cggccagaat gaagttgaag agcgggccgg ccagggcacc taccgggccg ccgaccgcat
     6721 aaccgatcaa accaacgact gcacccacaa agacaaagcc ggaattgctg acgtcggccc
     6781 gctgcgcgcg gtggagatgc cggctgtaga ggaggaagtc gtcgatcaat tcgtcggctt
     6841 cgtgacgcct gagaagttcg gtcgaagggg cctgtcggag ccagcttaga acggccttgg
     6901 ggagattgtc actctcgtcc ggctggagag gccatggagc ggcggcgagc caggcccgcc
     6961 actcaggatc agagacttgc tccggacgca gggccatcgg acgtccaggc tcctcgcgcc
     7021 acgagaatgt cgatctgctg ccggagagcc cgaatgtcgt tgcgcaccac gtccctacgt
     7081 cgccgataaa gagaaaaagc gatgtaagcg acgcagagca aggcgattgc gccagcggcg
     7141 accttgagga ccgggaaggt gtccagtggc gaagcgatgg gggggcgtac caatgccaag
     7201 aattgcaaca gttccatcga tgccacgacg cagatcgccg ttatcgaagc cgtgctggtc
     7261 agcaccaaat gccggtcgga ggcctggatc gcctccaatt gctcgcgcga cgtttcgagc
     7321 tgatttcgca tctcctgcgc tgacgaacga acgttcatgt ctttgccatt acctcatggc
     7381 cgccttggcc gtcaaagtct gcccgtcgcc ttgtcctggg cggcgaagat ctcgccgcag
     7441 ccgatccggg cgagtcggag cagccggagc agcccctcct cgtcatagca ttccccttcg
     7501 gcggtgacct gcgcctcgac gatgctgccg tcggcggtga ggacgaaatt gccgtccgcg
     7561 ccggcggtcg aatcctcctc gtaatcgagg tcgagcaccg cggcgccgcc gtggatgccg
     7621 cagctcaccg ccgcgacctt ctgccggatc gggtcggcgg ccaggagctg ctgctcgatc
     7681 agtcggtcga ccgcgatcct gagcgccacc caggctcccg agatggccgc cgtacgcgtg
     7741 ccgccgtcgg cctggatga
//
LOCUS       DASVQZ010000419        10842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_664769, whole genome shotgun sequence.
ACCESSION   DASVQZ010000419 DASVQZ010000000
VERSION     DASVQZ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664769"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VF547_11815"
     CDS             complement(<1..1029)
                     /locus_tag="VF547_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8263551.1"
                     /translation="MQGLNKGALICALSLVAGGSALAQGTSSTAPSEVPVSSMPSTIP
                     ASPPPPPPPLPSILPPSASAPSAGPPPASPTVLSPRSPRPAVLPPPSTDPLSIDPGQD
                     PILQLARSQASPEAFRSVVATAVERHPATQEARATSAEAEAVVDEAKERRLPSIDLSV
                     SSYKVISRDFSNDPQNILERSRPANRTDAILNVQQTVFDFGAGSERLLAAGARLRAAA
                     AEAEASADRIALHTVASWYDVFAYRALVALSESFVANQQELREAVEERVRQGASAPGD
                     VARVESYLASAQTRLAGFRRLLANAEARYTELTGSPPPPYLERAPVPQTPVLTRDAAA
                     LAAMSSPAA"
     gene            complement(1139..1579)
                     /locus_tag="VF547_11820"
     CDS             complement(1139..1579)
                     /locus_tag="VF547_11820"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX8263552.1"
                     /translation="MSAPATRPRAERRSLSDWLLLSEALAALTLASLAIAILPFRWVA
                     SAASARGPAKPRVDAEMVRRVRGAVQGWGRRVPWKAVCFQRGLALHWMLRRRGIRSVL
                     LYGARRQDDGLAAHVWVDVDGVTAIGGEEAPNFACLARFPPDGA"
     gene            1681..2850
                     /locus_tag="VF547_11825"
     CDS             1681..2850
                     /locus_tag="VF547_11825"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX8263553.1"
                     /translation="MHLPAEFMLIAACCRWPPSPERDRAVCAAAAGEIDWTLAAQIAE
                     RHRVEGLAWNALRQAGVAAPPEAAERIRAAAAGIARQNLVLTAESLRLAGRLEEAGIP
                     HLFVKGVALGALIYGSVGPKMGWDIDLLVPLASVEEAAAVLEAAGYRLDIPAGPKARE
                     RLARFHEHWKETVWRSEDRSLTVELHTRLADNPALLPRVGTESPVQRVEVAKGRFLPT
                     LGTDELFAYLCVHGASSAWFRLKWIADVGALLAPASPEEVERLYRRSQTLGAGRASAQ
                     ALLLCQRLFGTGLPPALAAELSADRMNRWLLAVALSKLAGRGLTAELHHVRLGTATIH
                     LMQLGLLPGLRFKLGELGRQLVNPDDRIAVPLPRALGFLYPFVSLARRLTRRPSG"
     gene            complement(2963..3658)
                     /gene="phoB"
                     /locus_tag="VF547_11830"
     CDS             complement(2963..3658)
                     /gene="phoB"
                     /locus_tag="VF547_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX8263554.1"
                     /translation="MTRGRILLVEDDRALVELIDFHLARAGYETTKTASGDEALLLVR
                     ESPPDLVLLDWMIEGTSGIEVCRQLGRMHKTRQIPVIMMTARGEEADRIRGLDTGADD
                     YITKPFSPREMLARIAAILRRVRPALTGATLTFEDLEMDVAAHRVRRGDKPVHLSPAE
                     FRLLRHFLESPRRVFTREGLLDTVWNDGSDLDERTVDVHIRRIRKALNEGGKPNLIRT
                     VRSAGYALDTAEG"
     gene            complement(3655..4359)
                     /gene="phoU"
                     /locus_tag="VF547_11835"
     CDS             complement(3655..4359)
                     /gene="phoU"
                     /locus_tag="VF547_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX8263555.1"
                     /translation="MATTGHTLKAFDDDLNQLRGSIAEMGGLAEAAIAAAMQALAARD
                     SDAARRVVEEDRRIDALEAEIERLAVQIIALRAPMADDLREVVAGLKIVGLVERIGDY
                     AKNIAKRVPAIEDSPNLRPLSLLPEMGRIVAEMVRSALDAFVARDPVKAAQVCERDKA
                     VDDFYNSIFRTLLTFMMESPNHITASTHLLFVAKNLERIGDHATNIAEMVYFAATGDH
                     LGERERGADTTRPVER"
     gene            complement(4371..5180)
                     /gene="pstB"
                     /locus_tag="VF547_11840"
     CDS             complement(4371..5180)
                     /gene="pstB"
                     /locus_tag="VF547_11840"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX8263556.1"
                     /translation="MIDEEERTAGAAPAAVASAPKMRARDVSVWYGEKQAIKQVSIDV
                     RPEHVTAFIGPSGCGKSTFLRTLNRMNDTIASARVSGGIELDGEDVYASGMDVVQLRA
                     RVGMVFQKPNPFPKSIYENVAYGPRIHGLAAGRQELDAIVERSLKRAGLWEEVKDRLA
                     ESGTALSGGQQQRLCIARAIAVDPEVILMDEPCSALDPIATAKIEELIHELRGRYAIV
                     IVTHNMQQAARVSQRTAFFHLGELLEYGETSEIFTNPRVERTKDYITGRYG"
     gene            complement(5186..6398)
                     /gene="pstA"
                     /locus_tag="VF547_11845"
                     /pseudo
     CDS             complement(5186..6398)
                     /gene="pstA"
                     /locus_tag="VF547_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
     gene            complement(6571..7953)
                     /gene="pstC"
                     /locus_tag="VF547_11850"
     CDS             complement(6571..7953)
                     /gene="pstC"
                     /locus_tag="VF547_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8263557.1"
                     /translation="MTPLALLVAILGLALLAWLSARARALAFAAPGAPRPHSLPGYHG
                     WYVAVWAAVPALLFLLLWVPLSDRLVMDAVMGSPAAANLPPFEMQRNAILQETRELAQ
                     GLRQASFYPEARELAPAYAAAEGRYRLIGTVAALLLAFAGGAFAYSRIGPAFRARTRV
                     ERLVMAALLLASLIAILTTLGIVLSLLFESLRFFSMISPVEFLFGTSWSPQTAIRSDQ
                     AGSSGAFGAVPLFWGTIFIGAIIAMIVAIPLGLMSAVYLTQYARPVVRRWMKPLLEML
                     AGVPTVVYGYFAALTVAPAIRDFGQSIGIPASSESALAAGLVMGIMIIPFVSSMADDS
                     LTAVPQSMRDGSLAMGATASETIRKVLVPAALPGIVGGVLLAVSRAIGETMIVVMAAG
                     LSANLTANPFASVTTVTTQIVQLLTGDQEFDSPKTLAAFALGLVLFIVTLLLNIVALR
                     VVKKYREAYD"
     gene            complement(8111..9145)
                     /locus_tag="VF547_11855"
     CDS             complement(8111..9145)
                     /locus_tag="VF547_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8263558.1"
                     /translation="MNKSYFAGAAALALVACGQQSDGGTQGQQIQIVGSSTVYPFTTA
                     VAEQVERTHPGMSAIVESTGTGAGLKLFCAGVGDQHPDMANASRRIKASEFQDCAKNG
                     VKQVIEVPVGIDGLALIEGKSSAGMSLTVRDIYAALAANPFGKPNTARTWKDVNPALP
                     AVAIQVYGPPPTSGTRDSLAELILEKGCESDPAMKALKESDKDRHKDLCTKVREDGAY
                     VEAGENDNLLVQKVAANPGAIGVLGYSFLEENADKVRGIPLGGVAPTAQTISSLTYPG
                     ARQLYLYAKGEHLNVIPGMKEFLAEYARASAKGGYLERRGLIPSPADVQAKGAEAATR
                     LEPMTADGLK"
     gene            complement(9233..10471)
                     /locus_tag="VF547_11860"
     CDS             complement(9233..10471)
                     /locus_tag="VF547_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8263559.1"
                     /translation="MPPPVPRRPVAAGLIVAMAVGAALLSGEALWAVLLGLIGGAAAA
                     LIGWRAHFFPEPSAPPPPPENGRDRWAREIVGAMSEPMLLVEGGRIVVANPAASQLLG
                     QWIEGQDVRLAMRHPATVERLARAQPPEAGIEQVEVVGLGQADRRWTMTVATLADGSR
                     LVHLDDRSEAIAAERMRVDFVANASHELRTPLATVLGFVETLQDKATAEDAEIRDRFL
                     GLMHGEGKRMQQLIEDLMSLSRIEAERFNPPRDVVDLVQLVEEVRAGCAQLLEERSNR
                     LVLENRAVSTVVPGDRGQLLQLVRNLVINAVKYGRSGSPVLVALEDDESSPMIRMSIA
                     DRGEGIAAVHLPRLTERFYRVDAGRSRAVGGTGLGLAIVKHIVSRHRGRLDIRSVVGE
                     GTTVLVSLPRGPDPQMPPLS"
     gene            10651..>10842
                     /locus_tag="VF547_11865"
     CDS             10651..>10842
                     /locus_tag="VF547_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8263560.1"
                     /translation="MTKSAFVAALLAASFATPASASQAPVDVAAELAALRARIAGLEA
                     EVAELKAAQSAPPAAPAALA"
BASE COUNT         1626 a         3748 c         3855 g         1613 t
ORIGIN      
        1 cgcggcgggc gagctcatcg ccgccagcgc ggccgcgtca cgggtgagaa ccggagtctg
       61 aggcaccggc gcccgctcca gatagggcgg aggcggcgac ccggtgagct cggtgtagcg
      121 cgcctcggca ttggcgagca gccgccggaa gcccgccagc cgggtctgcg ccgaagccag
      181 atagctctct acgcgggcga cgtcacccgg tgccgaggcg ccctggcgca cccgctcctc
      241 gaccgcctcg cgaagctcct gctggttggc gacgaacgac tcgctcagcg ccaccagcgc
      301 tcgataggcg aacacgtcgt accaggaagc cacggtatgg agtgcgatcc ggtcggcgct
      361 cgcttcggcc tcggcggcgg cggcgcgcag ccgggcgccc gccgccagga gccgctcgga
      421 gcccgctccg aagtcgaaga cggtctgctg gacgttgagg atcgcgtcgg tgcggttcgc
      481 cggccgcgag cgctcgagga tgttctgcgg gtcgttggaa aagtcgcggg agatcacctt
      541 gtaggaggag acgctgagat cgatcgaggg cagccgccgc tccttcgcct cgtcgaccac
      601 ggcctccgcc tcggcgctcg tcgcccgcgc ttcctgggtc gcgggatggc gctcgaccgc
      661 ggtcgcgacg acggaccgga acgcttccgg cgaggcctgg ctgcgggcga gctggaggat
      721 cgggtcctgg cccggatcga tgctgagcgg gtccgtcgag ggcggcggca gcaccgccgg
      781 gcgcggcgag cggggcgaaa ggacggtcgg ggaggccggc ggcggccccg ccgacggcgc
      841 tgaagcggaa ggcggcagga tcgagggcag aggcggcggc gggggcggcg gcgacgcggg
      901 aatggtcgac ggcatggagg agaccgggac ctcggacggc gccgtcgagg atgtcccctg
      961 ggcgagcgcc gacccgcccg cgacgagcga cagcgcgcag atcagcgctc ccttgttcag
     1021 cccctgcatg atcagatcga aaaccctctt gccgggggtg cttatcgccc ttcctcgcgc
     1081 gagactagga aaaccttcac cctcaaggga ttggggcggg cggctcgcgc tccgccgctc
     1141 aggccccgtc cggcgggaag cgagcgaggc aggcgaagtt cggcgcctcc tcgccgccga
     1201 tcgccgtcac gccgtcgacg tcgacccaga catgggcggc gaggccgtcg tcctggcggc
     1261 gcgctccgta gagaagcacc gagcggattc cgcgccggcg cagcatccag tgcagggcca
     1321 gcccgcgctg gaagcagacc gccttccacg gcaccctccg gccccagccc tggacggcgc
     1381 cgcgcacgcg ccggaccatc tcggcatcga cgcgcggctt ggccggaccg cgcgcggagg
     1441 cggcggaggc gacccaccgg aagggcagaa tcgcgatcgc cagcgacgcc agcgtcagcg
     1501 ccgccagggc ttcggagagc agcagccagt cggacaggct gcggcgctcg gcgcgcggtc
     1561 tcgtggcggg ggcggacatc gggagcgggt tacaggatcg gccgcgaggc tggcaaacta
     1621 ttgttcagcc gccgagtcat cgcctaggag cgacgcctac cagagaaagc cgcccgccgc
     1681 gtgcacctgc ccgccgaatt catgctcatc gccgcctgct gccgctggcc gccctcgccc
     1741 gaacgcgacc gggcggtttg cgccgcggct gccggcgaga tcgactggac gctggccgcc
     1801 cagatcgccg agcggcaccg agtcgaaggc ctcgcctgga atgcgcttcg ccaggccggc
     1861 gtcgccgccc cgcccgaggc ggcggagcgg atccgggccg cggcggcggg gatcgcgcgg
     1921 cagaatctcg tcctcaccgc cgaatcgttg cggctggcgg gtcgcctcga ggaggccgga
     1981 ataccgcatc tgttcgtcaa gggcgtcgcg ctcggcgcgc tgatctacgg ctcggtgggc
     2041 ccgaaaatgg ggtgggacat cgacttgctg gtgcccctgg cctccgtcga ggaggcggcg
     2101 gccgtgctgg aagcggcagg ctaccgcctc gacatccccg ccggaccgaa ggctcgggag
     2161 cggctcgcgc ggttccacga gcattggaag gagacggtct ggaggtccga ggaccgatca
     2221 ctgaccgtgg agcttcacac ccggttggcg gacaacccgg ccctcctgcc ccgggtcggc
     2281 accgagtcgc cggtccagcg ggtggaggtc gcaaaaggac gctttctgcc caccctcggc
     2341 accgacgagc tgttcgccta tctctgcgtc cacggggcgt cgagcgcctg gttccggctg
     2401 aagtggatcg cggacgtcgg cgccttgctc gcccccgcct cgcccgagga agtcgaacgc
     2461 ctctaccgcc gctcccagac gctcggcgcc ggacgggcct ccgcccaggc gctgctgctc
     2521 tgccagcggc tgttcggaac cggcttgccc cctgcgctcg cggccgagct gagcgccgac
     2581 cgcatgaacc gctggcttct cgccgtcgcc ctgagcaagc tcgccggccg cggcctcacc
     2641 gcggagctgc accatgtgcg cctgggcacc gccaccatcc acctgatgca gctgggcctg
     2701 ctgccgggcc tccggttcaa gctcggcgag ttgggccggc agctggtcaa ccccgacgac
     2761 cggatcgccg tcccgctgcc gcgcgcgctc ggcttcctct accccttcgt ctcgctggcc
     2821 aggcgcctga cccgccgccc gagcggctga cggcgccgcc gcctgcctcg agcacggcga
     2881 tccggaacgc atcgccgccc cgtgccggac tcgatcctgc ggcggcccga ggagcgctga
     2941 accgcctcgc ccttgcggcc gctcagcctt cggctgtgtc cagcgcgtat ccggccgagc
     3001 ggaccgtacg gatcagattg ggcttgccgc cctcgttcag agccttgcgg attcggcgga
     3061 tgtgcacgtc cacggtgcgc tcgtcgaggt cggagccgtc gttccagacc gtatccagca
     3121 gcccctcgcg cgtgaagacg cgccgagggc tttcgaggaa gtggcgaagg aggcggaact
     3181 cggccgggga caggtggacg ggcttgtccc cgcgccttac gcgatgggcg gcgacgtcca
     3241 tctcgaggtc ctcgaaggtc agggtcgccc ccgtcaaggc cggccgcacg cgtcgaagga
     3301 tcgcggcgat gcgggccagc atttcacggg gcgagaaggg cttggtgatg taatcgtcgg
     3361 cgccggtgtc gaggccgcgg atgcgatcgg cttcctcgcc ccgagcggtc atcattatca
     3421 cgggaatctg cctggtcttg tgcattcgtc cgagctgccg gcacacttcg atcccggacg
     3481 tgccttcgat catccagtcg agcaggacga ggtcgggcgg cgattcccgg accagcagca
     3541 acgcctcgtc gccgctggcg gtcttcgtcg tctcgtaacc cgccctggcg aggtggaagt
     3601 cgatcagctc gaccagcgcc cggtcgtcct cgaccagcag gatccggccc ctggtcaccg
     3661 ctcgaccggc cgcgtcgtgt ccgcgcccct ttcgcgctcg cccagatggt ccccggtggc
     3721 cgcgaaatag accatctcgg cgatgttggt ggcgtggtcc ccgatccgct ccaggttctt
     3781 ggcgacgaac aggaggtggg tcgaggccgt gatgtggttc gggctctcca tcatgaacgt
     3841 cagcagggtg cggaagatgc tgttgtagaa atcgtccacc gccttgtccc gctcgcagac
     3901 ctgggcggcc ttgacgggat cgcgcgcgac aaaggcgtcg agcgcgcttc ggaccatctc
     3961 ggcgacgatc cttcccattt ccggcagcag cgaaagcggc cgcagattgg ggctgtcctc
     4021 gatggcggga accctcttgg cgatgttctt ggcatagtcg ccgatgcgct cgaccaggcc
     4081 gacgatcttc aggccggcga cgacctcgcg cagatcgtcg gccatcggcg cccggagggc
     4141 gatgatctgg accgcaagcc gctcgatctc ggcttccagg gcgtcgatcc tcctgtcctc
     4201 ctcgacgacc cggcgggcgg cgtcgctgtc gcgggcggca agcgcctgca tcgccgcggc
     4261 gatcgccgcc tccgcgagcc cgcccatttc ggcgatgctg ccgcgcagct ggttgagatc
     4321 gtcgtcgaag gccttgagcg tgtgaccggt ggttgccatg tctgggttcc tcagccgtag
     4381 cggccggtga tgtaatcctt ggtcctctcg acccggggat tggtgaagat ctccgacgtc
     4441 tcgccatatt cgagcagctc gcccaggtgg aagaaggcgg tgcgctgcga aacccgggcc
     4501 gcctgctgca tgttgtgcgt gacgatgacg atcgcgtagc gcccgcgcag ctcgtggatc
     4561 agctcctcga tcttggcggt cgcgatcggg tccagcgccg agcagggctc gtccatcagg
     4621 atcacctcgg ggtcgacggc gatggcgcgg gcgatgcaga ggcgctgctg ctggccgccg
     4681 gaaagtgccg tcccgctctc ggccaggcgg tccttcacct cctcccacag gccggcgcgc
     4741 ttcagcgacc gctcgacgat cgcatcgagc tcctggcggc cggcggcgag gccgtggatg
     4801 cgcggcccat aggcgacgtt ctcgtagatc gacttgggga acgggttcgg cttctggaac
     4861 accattccga cccgggcgcg cagctgcacg acgtccatgc cgctcgcgta gacatcctcc
     4921 ccgtcgagct cgatcccgcc gctgacacgg gcgctggcga tggtgtcgtt catccggttc
     4981 aacgtgcgca ggaaggtcga cttgccgcag cccgacgggc cgatgaaggc ggtgacatgc
     5041 tccggccgga cgtcgatcga aacctgcttt atcgcctgct tctcgccgta ccagacgctc
     5101 acgtcgcgag cccgcatctt cggcgcgctc gcgacagcgg ccggggcggc gcccgcggtc
     5161 ctttcttcct cgtcgatcat cagcttcacc agcgcctctc gaacctgttc cgcaagtaga
     5221 tggcgacccc gttcatcgcc agcaggaaga ccaagaggac gatgatcgcc gccgaggtct
     5281 tctcgacgaa gccgcggctg acctcgtccg accagaggaa gatctgaacg ggcagaacgg
     5341 tggcggggtc ggcgatgccg cccggcggag tggcgatgaa ggcgcgcatt ccgatcatca
     5401 gcagcggcgc ggtctcgccg agcgcccggg ccatgccgat gatcgtcccg gtgaggattc
     5461 cgggcagggc cagcggcagg acgtggtggt agacgacctg gatccggctt gcgccgacgc
     5521 ccaaggcggc gtcgcggatc gacggcggca ccgacttgat cgcgttgcgg ccggcgatga
     5581 cgatcaccgg catggtcatc agcgccaggg tcaggccgcc gacgagcggc gccgagcgcg
     5641 gcatgtgcat ggtgttgagg aacaccgcca agcccaaaag cccgaagatg atcgagggca
     5701 ccgcggcgag gttgttgatc gagacctcga tgaggtcggt ccaccggttc ctcggggcat
     5761 attcctcgag atagattgcg gagagcacgc ccaccgggaa ggcgatggcg agagtgacca
     5821 gcatcgtcag cagcgagccc ttgaacgccc cccagatccc gaccgcggcc ggatccgtcg
     5881 cgtcggccga cgtcaggaag tcgaggttga agcgcgtgcg cacggagccg cgggcgccga
     5941 gcccgccgta gatgcgctcc gccgcgggat ctccctcgct cttcgcggcg acgtcgattt
     6001 ccgtggaagc cgggagccac aggctggcgg accgggcgag aagggacgga tccgcctcga
     6061 tcgcatcgag cacccccagc caggcggtat ccgcgaacag gcgtgcgcct ccgaccccat
     6121 attgcgcgtc cgcggccttc gcgaccgcgg cttgaaggtc ggaacccgcg agcgccgccc
     6181 tggcccccct ggagcgcacc gtcgccggat cgaggaatag gggcgccctg gcgagatcca
     6241 ggtcgagctg cacctcggtg cgggtgaagc cgcgggcgcc gttcgacacc atcgtgaaca
     6301 ggagcagggc caggaaggcc gcggagagca ggatcgcgcc gaggccgatc cagcggaagc
     6361 ggcgctcggc ggcgtagcgg cgcggtccgc ccgcgcatct ccggcgaccg ccagtcggtg
     6421 ggggtgcggg cggaactcgc ggaccctgcg ccgttcgggc tgagcttgtc gaagcccttc
     6481 gcttcctcgg aaaaagagaa ggaacggcct tcgacaggct caggccgaac ggtgttcttc
     6541 tcgggcccgg ggcgggtctt gtcgcggtcg tcagtcataa gcctcgcgat atttcttcac
     6601 gacgcgaagg gcgacgatgt tgagcagcag ggtgacgatg aacaggacca ggccgagcgc
     6661 gaaggcggcc agcgtcttgg ggctgtcgaa ctcctggtcg ccggtcagca gctggacgat
     6721 ctgcgtcgtc accgtggtga cgctcgcgaa cggattggcg gtgaggttgg cggagaggcc
     6781 ggccgccatg acgacgatca tcgtctcgcc gatcgcccgg ctgacggcga gcaggacccc
     6841 gccgacgatg ccgggcaagg cggcggggac gagcaccttg cggatcgtct cggaggcggt
     6901 cgcgcccatc gccagcgatc cgtcccgcat cgactggggc acggcggtca gcgaatcgtc
     6961 cgccatcgag gagacgaagg ggatgatcat gatccccatc accaggcccg ccgcgagcgc
     7021 gctttccgaa gaggccggga ttccgatcga ctggccgaaa tcgcggatcg ccggggccac
     7081 cgtcagagcc gcgaaatagc cgtagacaac ggtcggaacg ccggcgagca tctccagcag
     7141 cggcttcatc cagcgccgaa cgacggggcg ggcgtattgg gtgaggtaga cggcgctcat
     7201 cagtccgagc gggatggcga cgatcatcgc gatgatcgcg ccgatgaaga tcgtgcccca
     7261 gaagagcggg accgcgccga acgcccccga cgagccggcc tggtcggacc ggatcgccgt
     7321 ctgcgggctc cagctcgttc cgaacaggaa ctcgaccgga gatatcatgc tgaagaagcg
     7381 cagcgattcg aagaggagcg agaggacgat tccgagggtc gtcaggatcg cgatcaggga
     7441 cgccagcaga agcgccgcca tcaccagccg ctcgacccgg gtgcgggcgc ggaaggccgg
     7501 gccgatccgg ctgtaggcga aggcgccgcc ggcgaaggcg agcagcagcg cggcgacggt
     7561 gccgatcagc cggtagcggc cctccgcggc ggcgtaggcc ggcgcgagct cgcgcgcctc
     7621 gggatagaag ctcgcctggc gcagcccctg cgccagctcg cgggtctcct ggaggatcgc
     7681 gttgcgctgc atctcgaagg gcgggaggtt cgccgccgcc gggctgccca tcaccgcgtc
     7741 catgacgagc cggtcgctca gcggcaccca aaggagcagg aagagcagag cgggcaccgc
     7801 cgcccagacc gcgacgtacc agccatggta gccgggcagc gaatggggcc gcggggcgcc
     7861 cggcgcggcg aaggccaggg cgcgggcgcg agccgacagc caggcaagca aggcgaggcc
     7921 gaggatggcg acgaggagcg cgaggggggt cacctcccta atcctccctg ccgcgcagcg
     7981 gtggggaggg ggaccacccg aagggtggtg gaggggtact tgacgaggcc tgttccattt
     8041 acccctccac cgcgcttcgc gcggtccccc tcccccggaa atccggggaa ggaattggtc
     8101 gccgtcatcc tcacttcagc ccgtcggccg tcatcggctc cagcctcgtc gccgcctcgg
     8161 cgcccttggc ctggacgtcg gccggggagg ggatgaggcc gcggcgctcg agatagccgc
     8221 ccttcgccga ggcgcgggcg tattcggcga ggaactcctt catccccggg atgacgttca
     8281 gatgctcgcc cttcgcgtag agatagagct ggcgggcacc ggggtaggtg aggctggaga
     8341 tggtctgcgc ggtgggagcg accccgccga gcgggatgcc cctcaccttg tccgcattct
     8401 cctcgaggaa ggaatagccg agcacgccga tcgcgccggg gttggcggcg accttctgga
     8461 cgagcagatt gtcgttctcg ccggcttcga cataggcgcc gtcctcgcgc accttggtgc
     8521 acagatcctt gtgcctgtcc ttgtcgcttt ccttcagagc cttcatcgcc gggtcgctct
     8581 cgcagccctt ctcgaggatc agctcggcga ggctgtcgcg ggtgcccgag gtcggcggcg
     8641 ggccgtagac ctggatggcg accgcgggca gcgcggggtt gacgtccttc caggtcctgg
     8701 ccgtgttggg cttgccgaac gggttcgccg ccagcgccgc gtagatgtcg cggacggtga
     8761 ggctcatccc ggcgctgctc ttgccctcga tcagcgccag cccgtcgatt cccaccggca
     8821 cctcgatgac ctgcttcacc ccgttcttcg cgcaatcctg aaattcggac gccttgatcc
     8881 gccgcgaggc gttggccatg tccggatgct ggtcgccgac gccggcgcag aacagcttca
     8941 ggccggcccc ggtgccggtc gattcgacga tcgcgctcat ccccggatga gtgcgctcga
     9001 cctgctcggc gaccgccgtc gtgaacgggt agaccgtgga ggagccgacg atctggatct
     9061 gctgcccctg cgtcccgccg tccgattgct gcccgcaggc caccagggcc agcgcggccg
     9121 cgccggcaaa gtagctcttg ttcattcatg cctccgtcga ccccggtggc ggggccccgg
     9181 cgacctccct aggccggttc ggcgagagtc ctgtgactgt tcgatgacgg tttcatgaca
     9241 agggcggcat ttgcggatcc ggcccccgcg gcagcgagac gagcaccgtc gtgccctcgc
     9301 cgacgacgct ccggatgtcc agccggcccc ggtggcggct gacgatatgc ttgacgatgg
     9361 cgagcccgag cccggtcccg ccgaccgccc ggctccgccc ggcatcgacg cgatagaagc
     9421 gctccgtgag tcgggggagg tgcacggcgg cgattccttc gccgcgatcg gcgatgctca
     9481 tccggatcat cggcgagctc tcgtcgtcct cgagggcgac caggacgggc gatccggagc
     9541 ggccatattt gacggcattg atgacgagat tgcggacgag ctgcagcaat tgcccccggt
     9601 cccccggaac gaccgtcgac acggcccgat tttccaggac gagccggttc gaccgctcct
     9661 cgagcagctg ggcgcagccg gcccggacct cctcgacgag ctggacgaga tccacgacgt
     9721 cgcgcggcgg attgaaccgt tctgcctcga tccgcgacag cgacatcaga tcctcgatga
     9781 gctgctgcat gcgcttgccc tcgccatgca tcaggccgag gaagcggtcc ctgatctcgg
     9841 catcctcggc cgtcgccttg tcctgcagcg tctcgacgaa gccgaggacg gtggcgagcg
     9901 gggtgcgcag ctcgtggctg gcattggcga cgaaatcgac ccgcatccgc tcggcggcga
     9961 tcgcttcgct gcggtcgtcc aggtggacga gccgggaacc gtcggcgagc gtggcgacgg
    10021 tcatcgtcca tcgccggtcc gcctggccga ggccgaccac ctcgacctgc tcgattccgg
    10081 cctcgggcgg ctgcgcgcgg gccaggcgct ccaccgtcgc ggggtgccgc atggccagcc
    10141 gcacgtcctg gccctcgatc cactggccca gcaattgcga ggcggccgga ttggcgacga
    10201 cgattcgccc gccttcgacc aggagcatcg gctccgacat cgctccgacg atttcgcgcg
    10261 cccagcgatc gcggccgttt tccggcggcg gcggaggcgc gctcggctcg gggaagaaat
    10321 gggcccgcca gccgatgagc gcggccgcgg cccccccgat caggcccagg agcaccgccc
    10381 agagcgcttc ccccgacagc agggcggcgc cgacggccat tgcgacgatg aggcccgcgg
    10441 cgacgggtcg gcgggggacg ggcggcggca tgcgcctgcc ttagcgggct tcggggatga
    10501 agttccaggc gggccgcctt ccatacgggg ccggcgggcc tgcgctccgc gacgagaagc
    10561 gaatgccgtc ataaaagtgt catccggatg actcggaacc gcaatccgac cgtcctaaag
    10621 cctgtctcgc cacctgattg gagctgccgc atgaccaagt ccgccttcgt tgccgccctt
    10681 cttgcagcca gcttcgccac ccccgcttcg gcgtcgcaag cgcccgtcga cgtggcggcc
    10741 gagcttgcgg cgcttcgggc gaggatcgcg ggcctggaag ccgaagtggc cgagctgaag
    10801 gccgcgcaaa gcgctccgcc ggcagcgccc gcagcgctcg cc
//
LOCUS       DASVQZ010000420         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_377037, whole genome shotgun sequence.
ACCESSION   DASVQZ010000420 DASVQZ010000000
VERSION     DASVQZ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377037"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VF547_11870"
     CDS             complement(<1..434)
                     /locus_tag="VF547_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263561.1"
                     /translation="MKFRTLALRIGIIAAAMVAGTGTAQAQPNPVLYLTGLEYYSAGG
                     KNWVRHRYDVMNKDQYPAAMFAAAPALPPCGTNTNSSRSWVDFFDSRGKRLYGFCALG
                     DPANLGKIWFATEEGTVPPSWVYIEITDRQTNTKYKSNLAETT"
     gene            579..1574
                     /gene="hemB"
                     /locus_tag="VF547_11875"
     CDS             579..1574
                     /gene="hemB"
                     /locus_tag="VF547_11875"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8263562.1"
                     /translation="MSNAAFPALRLRRTRAAAWSRALFAETVLTPADLIWPLFIAGGE
                     GAEEPVGSLPGVSRWSVDRLAAKAREARDLGIPCIALFPNTPPGLRTADAREALNRDN
                     LICRAVRAVKEAAPEVGVLTDVALDPYTAHGHDGLVDEAGYVLNDSTSAVLVEQALVQ
                     AEAGADIVAPSDMMDGRVGAIRAALEREGHVNVQIMAYAAKYASAFYGPFRDAVGSRG
                     LLKGDKKSYQMDPANGEEALREVALDLAEGADSVMVKPGLPYLDIVRRVKERFEVPVF
                     AYQVSGEYAMIEAAAAAGAGERDALVLETLMAFKRAGCSGVLTYHAPLAARLLAR"
     gene            1571..3829
                     /locus_tag="VF547_11880"
     CDS             1571..3829
                     /locus_tag="VF547_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX8263563.1"
                     /translation="MTEAPAESAAEGSRERFAKPLFLVTVLTGSFLLFLTQPMIARMA
                     LPRLGGAPAVWNSAMLVYQGLLLAGYAYAHFLTRLRPRRQAGLHLALFALAALWLPIG
                     LSNATLPADADAALWAPWFLASSIGPLFFIVSAQAPLMQRWFALEGARGDPYPLYAAS
                     NLGSFAGLLSYPLLVEPLLTLEQQSLVWSAGYALLVLMVALCALTVPPAAVEAMPEAA
                     GPAPSWRRLLHWVALAAVPSGLMLSTTTHLTTDIVAVPLLWVLPLALYLLSFVIAFAA
                     RRRPADIVVAIAPLFLLVAGGFAFSRGTELPFFTATLGLVLLLIVAVALHAEMYRLRP
                     EAAHLTAFYLAMAVGGVLGGIFCAILAPTVFDWAYEHPLLIAAAALLVPQSPFLPWAA
                     RMHPALRWALVLFALALSWAADSRMGPFDNDVTALVGSIAISLIALNFIGSRAAFALG
                     VVALMMSYGGWTKVEESRMGIRTRSYFGIYTVSFRFNPPTRQLTHGTTLHGSQNLTAG
                     MEAEPTTYYAPRSGVGHALASLPIFHGPNPAVGVVGLGAGTLSCFARPGQDWRFFEID
                     PAMVRIASDPHRFSFLSRCAPKAKIVLGDARLSLAREPAGRLDLLAVDAFSSDAIPIH
                     LLTREALQVYRRALKPDGLLLVHISNRYLDLEPVLAAAAKRDGWHAAALDYSPSALEE
                     GRNLSRSIWVAMARDRDTLLTVGIASGDDAHLWRPLRSRPGFTGWTDDYASILPVLEE
                     WRAWLPAVLKGQ"
BASE COUNT          503 a         1427 c         1311 g          672 t
ORIGIN      
        1 gtggtctccg cgaggttcga cttgtacttc gtgttggtct gccggtcggt gatctcgata
       61 tagacccagc tcggcggaac cgtcccttcc tcggtcgcga accagatctt gccgaggttg
      121 gccggatcgc cgagcgcgca gaagccgtag aggcgcttgc cgcgggaatc gaagaagtcg
      181 acccaggagc gcgacgagtt ggtgttggtc ccgcagggcg gcagcgccgg cgcggccgcg
      241 aacatcgcgg ccggatactg atccttgttc atcacgtcgt agcggtggcg gacccagttc
      301 ttccctccgg ccgaataata ttcgaggccg gtgaggtaga ggacgggatt gggctgggcc
      361 tgcgccgtcc cggtcccggc aaccatcgca gccgcgatga taccgatgcg caatgcgagc
      421 gttctgaact tcatcgactg ccctttcatc cccaagagtg agcctggaag tctgtattgg
      481 aatatgaaga atgccatcgt tccgggtgcg acacaaggaa gtcgctgtgc cgcccgccgc
      541 gctttgcagg ggcgccaagg gacgttacag ccgcgcccat gagcaatgcc gccttccccg
      601 cgcttcggct ccgccgcacc cgcgccgcgg cctggagccg ggccttgttc gcggagaccg
      661 tgctcactcc cgcggacctc atctggccgc tgttcatcgc cggaggggag ggggcggagg
      721 agccggtggg cagcctgccg ggcgtgtcgc gctggtcggt cgaccggctc gcagcgaaag
      781 cccgcgaggc gcgcgacctc ggcatcccgt gcatcgccct gtttccgaac acgccgcccg
      841 ggcttcgcac cgccgacgcc cgcgaggcgc tcaaccgcga caatctcatc tgccgcgcgg
      901 tccgggccgt gaaggaggcg gcgccggagg tcggcgtcct caccgacgtc gccctcgatc
      961 cctatacggc gcacggccac gacgggctgg tcgacgaggc cggctacgtg ctcaacgatt
     1021 cgaccagcgc ggtcctggtc gagcaggccc tcgtccaggc ggaggcgggg gccgacatcg
     1081 tcgcgccttc cgacatgatg gacgggcggg tcggagcgat ccgggccgcg ctcgagcgcg
     1141 agggccatgt caacgtccag atcatggcct atgccgccaa atatgcctcc gccttctacg
     1201 gcccgttccg cgacgcggtg ggctcgcgcg gcctcctgaa gggcgacaag aagagctacc
     1261 agatggatcc ggccaatggc gaggaggcgc ttcgcgaggt cgcgctcgac ctcgccgagg
     1321 gcgccgacag cgtcatggtc aagcccggcc tgccctatct cgacatcgtc cggcgggtga
     1381 aggagcggtt cgaggtgccg gtcttcgcct atcaggtgtc gggcgaatat gcgatgatcg
     1441 aggcggccgc ggccgccggc gccggcgagc gcgacgctct ggtgctggag acgctgatgg
     1501 cgttcaagcg ggcgggctgc tcgggcgtcc tcacctacca cgcgccgctg gcggcgaggc
     1561 tgctggcccg gtgacggagg ccccggcgga gagcgcggcg gagggctcgc gagagcgttt
     1621 cgcgaagccg ctcttcctcg tcaccgtcct gaccgggtcc ttcctcctct tcctcaccca
     1681 gccgatgatc gcccggatgg cgcttccccg gctgggcggg gctccggcgg tgtggaacag
     1741 cgcgatgctg gtctaccaag gcctgctcct ggccggctac gcctacgccc atttcctcac
     1801 ccggctgagg cctcggcggc aggccggcct ccacctcgcc ctgttcgcgc tcgccgcctt
     1861 gtggctgccg atcgggctca gcaacgccac tctccccgcc gatgccgacg ccgccttgtg
     1921 ggcgccctgg ttcctcgcca gctcgatcgg gccgctcttc ttcatcgtct cggcacaggc
     1981 gccgctgatg cagcgctggt tcgcgcttga gggcgcgcgg ggcgatccct atccgctcta
     2041 cgccgcgtcc aatctcggca gcttcgccgg cctgctttcc tatcccctgc tggtcgagcc
     2101 gcttctgacg ctggagcagc agagcctggt ctggtcggcg ggctatgccc tgctcgtcct
     2161 gatggtcgct ctttgcgccc tcaccgtgcc gcccgcggcg gtcgaggcga tgccggaggc
     2221 ggccgggccg gcgccgtcct ggcggcgcct gctccactgg gtggcgctgg cggcggtgcc
     2281 gtcggggctg atgctctcga ccaccaccca tctcaccacc gacatcgtcg ccgtccccct
     2341 gctctgggtg cttccgctgg ccctctacct gctcagcttc gtcatcgcct tcgccgcgcg
     2401 gcgccggccc gccgacatcg tcgtcgcgat cgctcctctc ttcctgctcg tcgccggcgg
     2461 cttcgcgttc agccgcggca ccgagctgcc cttcttcacc gcgaccctgg ggctcgtgct
     2521 cctgctgatc gtcgcggtcg cgctccacgc cgagatgtac cggctgcggc cggaggcggc
     2581 ccatctcacc gctttctacc tggcgatggc ggtcggcggg gtgctcggcg gaatcttctg
     2641 cgcgatcctc gccccgaccg ttttcgactg ggcctacgag catccgctgc tgatcgccgc
     2701 cgccgccctt ctcgtcccgc aatcgccctt cctgccctgg gcggcgcgga tgcatccggc
     2761 gctgcgctgg gccctggtcc tcttcgccct cgccctttcc tgggcggcgg acagcaggat
     2821 ggggccgttc gacaacgacg tgaccgccct ggtcggaagc atcgccatct ctctgatcgc
     2881 gctcaacttc atcggctcga gggcggcctt cgcgctcggc gtcgtggcgc tgatgatgag
     2941 ctacggcggc tggaccaagg tcgaggagag ccggatgggg atccgcaccc gctcctattt
     3001 cggaatctac acggtcagct tccgcttcaa tccgccgacc cgccagctca cccacggcac
     3061 cacgctgcac gggtcgcaga acctgacggc ggggatggag gcggagccga ccacttatta
     3121 tgcgccccgc tccggcgtcg gccacgcgct cgcctccctg ccgatcttcc acgggccgaa
     3181 tccggcggtc ggggtggtcg ggctgggcgc cggcaccctg tcctgcttcg cccggcccgg
     3241 gcaggactgg cgcttcttcg agatcgaccc ggcgatggtc aggatcgcgt ccgatccgca
     3301 ccgcttctcc ttcctgtcgc gctgcgcccc gaaggcgaag atcgtgctgg gcgacgcccg
     3361 cctgagcctc gcccgcgagc ccgccgggcg gctcgacctg ctcgccgtcg acgccttctc
     3421 ctccgacgcc atcccgatcc acctgctcac ccgcgaggcg cttcaggtct accggcgcgc
     3481 cctcaagccc gacgggctgc tgctcgtcca tatctccaac cgctatctcg acctcgagcc
     3541 ggtgctggcg gcggcggcga agcgcgacgg ctggcacgcc gccgcgctcg attattcgcc
     3601 gagcgcgctg gaggaagggc gcaacctcag ccgctcgatc tgggtggcga tggcgcgcga
     3661 ccgcgacacc ttgctgaccg tgggaatcgc cagcggcgac gacgcccacc tctggcggcc
     3721 gctgcgcagc cggccggggt tcaccggctg gaccgacgac tatgcgagca tcctgccggt
     3781 gctcgaggaa tggcgggcct ggctgcccgc ggtgctcaag gggcaatgat cgagaccgcg
     3841 cgcctccggc tcaggaactg gcgcgaggag gatatcgaac ccttcgtccg ccacctcaac
     3901 acggagccgg tga
//
LOCUS       DASVQZ010000421         6808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_952385, whole genome shotgun sequence.
ACCESSION   DASVQZ010000421 DASVQZ010000000
VERSION     DASVQZ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952385"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF547_11885"
     CDS             complement(<1..382)
                     /locus_tag="VF547_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263564.1"
                     /translation="MAQDSPERGRMDLQEALGASRAATLQKFSLYLPDRDRDGAPVPH
                     LDAWHAAAMTLFAEVNGGATLMPPADGIWNPEGGGEMVREATRIVYSFVRDGEKFERD
                     IEQLAVFIHSFGRHSKQGEVMVEFA"
     gene            complement(395..763)
                     /locus_tag="VF547_11890"
     CDS             complement(395..763)
                     /locus_tag="VF547_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263565.1"
                     /translation="MNEIAKVNPKRGRVPGRKAAPADMAWVDRLEPLDPDATAAALER
                     PGWPIPPGYYTPDTLPPLSEEARKILASVAVGVAGLGAKRAAERAGAAAGRPGITLED
                     EGQPGIALSDEGLPGFDPGP"
     gene            947..3631
                     /gene="acnA"
                     /locus_tag="VF547_11895"
     CDS             947..3631
                     /gene="acnA"
                     /locus_tag="VF547_11895"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX8263566.1"
                     /translation="MTAVGHDTLKTRTQLQVGPKRYAYYSLEKAAAALGDVSRLPFSM
                     KVLLENLLRFEDGKTVTRADIQALVDWQKERSSTKEIQYRPARVLMQDFTGVPAVVDL
                     AAMRDAMARLGGRPERINPQVPVHLVIDHSVMVDAFGTPQSFARNVELEYHRNMERYE
                     FLKWGSKAFDNFEVVPPGTGICHQVNLEYIARGVWSSKDPSGELVAYPDTLVGTDSHT
                     TMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFHLTGSLQEGITATDLVLTVTQ
                     MLRARGVVGRFVEFYGEGVSALSVADRATIANMAPEYGATCGFFPVDGKTLDYMRLTG
                     RSEDQIALTEAYAKAQGLWLEPGAYHEPLFTDTLELDMDGVEPSLAGPKRPQDKVPLS
                     RVDEEFVANFTKEYGHSLAELEQRHPVAGDAAVPDIGHGDVVIAAITSCTNTSNPAVL
                     IAAGLVARKARERGLDSKPWVKTSLAPGSQVVTDYLDRAGLSEDLNAIGFDLVGYGCT
                     TCIGNSGPLPEPISNAIARHDLVAASVLSGNRNFEGRVSPDVRANYLASPPLVVAYAL
                     KGTVAGDMIEEPLGTGSDGEPVFLKDVWPTNEEVRRLIDAHVHGDMFRSRYADVYKGD
                     DKWRSIPVAGGETYAWSAGSTYIQNPPYFEGMTMTPTPPSDIESARPLAIFGDSITTD
                     HISPAGAIKADSPAGTYLSEHQVARGDFNSYGARRGNHEVMMRGTFANIRIKNRMIPG
                     TEGGLTRHIPSGEILPIYDAAMRYKAEGTPLVVIAGKEYGTGSSRDWAAKGTNLLGVR
                     AVVAETYERIHRSNLVGMGVVPLQFVGDVPIFEGTETFTIKGIADLQPRQEIEVRVAR
                     ADGSTFAFTARVRIDTLNELEYFRAGGILHYVLRKLAA"
     gene            3651..3983
                     /locus_tag="VF547_11900"
     CDS             3651..3983
                     /locus_tag="VF547_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263567.1"
                     /translation="MITWLLAAMAPAGPGYEPPPQVFYPQAVACAVSAKLAAGKAMDG
                     DRFGELMTWGMILADVGRKAGRTKAQVDSADLVAAEPFYKHLKEKKPKAFAAHRAYCR
                     ALLDADRP"
     gene            3967..4611
                     /locus_tag="VF547_11905"
     CDS             3967..4611
                     /locus_tag="VF547_11905"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX8263568.1"
                     /translation="MLIDLSHAIEDGMVTYRGLPAPHICDYWKREDSAANYDDGSTFQ
                     IGRIDMVANTGTYLDTPFHRYADGDDLAAVGLERLAGLDGLCVPAEGMEAGAELFEGL
                     DVAGKAVLVRTGWDRHWRTDAYFSDHPYLSEGAARLLAERGAALVGIDSYNIDDTRTR
                     RRPIHTVLLGAGTLIVEHMTNLAALPPSGFRFTAAPPKVAGMGTFPVRAFAEIG"
     gene            4673..5146
                     /locus_tag="VF547_11910"
     CDS             4673..5146
                     /locus_tag="VF547_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8263569.1"
                     /translation="MLHFTLAALLAAAAPANPQIDYPGFRELALDVEDYRRSRLVDWK
                     GFTAAAAEPDALILDARSASAFARGHIEGAVNLPFTDFTDESLAAVIGRRDRPILIYC
                     NNNFRNDAPPVQLKSVRLALNIQTFINLVGYGYANVRELNEVIDFNDPKVRWVKG"
     gene            complement(5127..5852)
                     /locus_tag="VF547_11915"
     CDS             complement(5127..5852)
                     /locus_tag="VF547_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX8263570.1"
                     /translation="MAATTLIDRAVLRLSGEDVLGFLQGLVTHDLALLAPDRPLWAGL
                     LTPQGKALFDFILWADGGDVLIDCEAEAAGDLAKRLTLYRLRRKIAIEPDPVLRVHWA
                     PEGPGPADPRLAELGHRWLAAENGAGGQDWLAHRLALGVTEGRAELGSDKTLWLECNA
                     AELDGVSFTKGCYVGQENTARMNYRSKVNRRLVVVPAEGPGERARVHYPGLGLSVEHR
                     RVEDLGGAIVPPWLAAALSPSPT"
     gene            5894..>6808
                     /gene="pyrC"
                     /locus_tag="VF547_11920"
     CDS             5894..>6808
                     /gene="pyrC"
                     /locus_tag="VF547_11920"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX8263571.1"
                     /translation="MTEPTRLILRRPDDWHVHLRDGDMLAAVAPSTARQFARAIVMPN
                     LDPPVTTVAAARAYRDRILEAVGRARFTPLMTCYLTDEADPAELASGFEAGVFAAAKL
                     YPAHATTNSAHGVTAVDRIHRALEAMERVGMPLLIHGEVVDPDVDIFDRERLFIDRVF
                     SRLVRDFPALKMVFEHITTADAADFVLAAGPNVAATVTPHHLHINRNALFAGGLRPHA
                     YCLPVAKREAHRIAVRRAATSGSPQFFLGTDSAPHAAERKESDCGCAGIFNAPFALES
                     YADAFEEEGALERLEAFSSENGPRFYGLP"
BASE COUNT         1080 a         2516 c         2153 g         1059 t
ORIGIN      
        1 cggcaaattc caccatcacc tcgccctgct tcgaatgcct cccgaagctg tggatgaaga
       61 ctgcgagctg ctcgatgtcg cgctcgaatt tctcgccatc ccgaacgaag gagtagacga
      121 ttctcgtcgc ctcacggacc atctccccgc cgccttccgg gttccagatg ccgtcggcgg
      181 gcggcatcaa cgtggctccc ccattcacct ccgcgaacaa ggtcatcgcc gcggcatgcc
      241 aggcgtccag atgcggaacg ggcgcgccgt cgcggtcgcg gtcgggcagg taaaggctga
      301 acttctggag ggtggccgct cggctcgcgc ccaatgcctc ctggaggtcc atccgcccgc
      361 gttccgggct gtcctgagcc acgggagcgg aaggctatgg gccgggatcg aaaccgggaa
      421 gcccctcgtc agacagagcg atccccggtt gcccttcatc ttcgagggtt atgccgggac
      481 ggcccgccgc ggctccggcc cgctctgcgg cgcgcttcgc gccgagccca gcaaccccta
      541 cggcaacgga agccaggatc tttcgcgcct cctcgctcag cggcggtaga gtgtcgggcg
      601 tgtagtagcc cgggggaatg ggccaccctg ggcgttccag agccgccgct gtcgcgtccg
      661 ggtccagcgg ctcgagccga tccacccaag ccatgtcggc cggggcggcc ttccggcccg
      721 gaacacggcc gcgcttagga ttgacctttg cgatctcgtt cacgagcgac tcctcggcga
      781 ctctgtagac agttcctcgt cgcttttcaa cctaaccttc gcctcaggct gtcgtttttc
      841 gcaaccgggc cgcgcccttt caactgcgcc gctcgcttgc tagagcgccg ccaacacttg
      901 ccgggtggca acagtctcca tccgtcaaca attcgaaagg cctccgatga cagccgtcgg
      961 ccacgacacc ctgaaaaccc gcacccagct tcaggtcggt ccgaagcgtt acgcctacta
     1021 ctccctcgag aaagccgccg ccgccctcgg cgacgtctcc cgcctcccgt tcagcatgaa
     1081 ggtcctgctc gagaacctgc tccgcttcga ggacggcaag accgtcaccc gcgccgacat
     1141 ccaggcgctg gtcgactggc agaaggagcg gtcctccacc aaggagatcc agtaccgccc
     1201 ggcccgagtc ctcatgcagg acttcaccgg ggtccccgcc gtcgtcgacc tcgccgcgat
     1261 gcgcgacgcc atggcccggc tcggcggtcg gcccgagcgg atcaacccgc aggtgccggt
     1321 ccacctcgtc atcgaccatt cggtcatggt cgacgccttc gggacgccgc agtccttcgc
     1381 ccgcaacgtc gagctcgaat atcaccgcaa catggagcgc tacgagttcc tgaagtgggg
     1441 ctcgaaggcg ttcgacaatt tcgaggtggt gccgcccgga accggcatct gccaccaggt
     1501 caatctcgaa tatatcgccc ggggcgtctg gtcgtcgaag gacccgtccg gcgagctcgt
     1561 cgcctatccc gacacgctgg tcgggaccga cagccacacg accatggtca acggcctcgg
     1621 cgtgctcggc tggggcgtcg gcgggatcga ggcggaagcc gcgatgctcg gccagccggt
     1681 ctcgatgctg atccccgagg tggtcggctt ccatctgacc ggctcgcttc aggaggggat
     1741 caccgccacc gacctcgtcc tcaccgtcac ccagatgctc cgcgcccgcg gggtggtcgg
     1801 ccgcttcgtc gagttctacg gcgagggcgt ctcggctttg tcggtcgccg accgggcgac
     1861 gatcgccaac atggcgcccg aatatggcgc cacctgcggc ttcttcccgg tcgacggcaa
     1921 gacgctcgac tacatgcgcc tcaccggccg ctcggaggat cagatcgcgc tcaccgaggc
     1981 ctatgccaag gcccagggcc tgtggctcga gccgggcgcc tatcacgagc ccttgttcac
     2041 cgacacgctc gagctcgaca tggacggggt cgagccgtcg ctggccggcc cgaagcggcc
     2101 gcaggacaag gtgccgctct cgcgggtcga cgaggagttc gtcgccaatt tcaccaagga
     2161 atatggccat tcgctggccg agctcgagca gcgccacccg gtcgccggcg acgccgccgt
     2221 gcccgacatc ggccatggcg acgtcgtcat cgccgccatc acctcctgca ccaacacctc
     2281 caatcccgcc gtcctcatcg ccgccggcct cgtcgcccgc aaggcgcgcg agcgcgggct
     2341 cgattccaag ccctgggtga agacctcgct ggcgccgggc tcgcaggtgg tcaccgacta
     2401 tctcgaccgc gccggcctgt cggaggacct gaacgcgatc ggcttcgacc tggtcggcta
     2461 cggctgcacc acctgcatcg gcaattcggg gccgctgccc gagccgatct ccaacgcgat
     2521 cgccaggcac gacctcgtcg ccgcctcggt cctctcgggc aaccgcaatt tcgagggccg
     2581 ggtgtcgccg gacgttcgcg ccaactatct cgcctcgccg ccgctggtgg tcgcctatgc
     2641 cctgaagggc acggtcgcgg gcgacatgat cgaggagccg ctcggcaccg gctcggacgg
     2701 ggagccggtc ttcctcaagg acgtctggcc gaccaacgag gaggtccgcc ggctgatcga
     2761 cgcccacgtc cacggcgaca tgttccgctc gcgctacgcc gacgtctaca agggcgacga
     2821 caagtggcgc tcgatcccgg tcgcgggcgg cgagacctac gcctggtcgg ccggctcgac
     2881 ctacatccag aacccgccct atttcgaggg catgacgatg actccgactc cgccttcgga
     2941 catcgagtcg gctcggcctc tggcgatctt tggcgattcc atcacgaccg accacatctc
     3001 cccggccggc gcgatcaagg ccgacagccc ggccggcacc tatctctccg agcaccaggt
     3061 cgcccgcggc gatttcaact cctacggcgc ccgccgcggc aaccatgaag tgatgatgcg
     3121 cggcaccttt gccaacatac gcatcaaaaa ccgaatgatt ccaggcaccg aaggcggttt
     3181 gacccgccac atcccgtcgg gcgagatcct gcccatctac gacgcggcga tgcgctacaa
     3241 ggcggagggg acgccgctgg tcgtgatcgc cggcaaggaa tatggcaccg gctcctcccg
     3301 cgactgggcc gccaagggca ccaacctgct cggggtccgc gccgtcgtcg ccgagaccta
     3361 tgagcggatc caccgctcca acctggtcgg gatgggcgtc gtcccgctcc agttcgtcgg
     3421 cgacgtgccg atcttcgagg gcacggaaac cttcacgatc aaggggatag ccgaccttca
     3481 gccgcgccag gagatcgagg tccgggtcgc cagggcggac ggctccacct tcgccttcac
     3541 cgcgagggtg cggatcgaca cgctcaacga actcgaatat ttccgcgccg gcgggatcct
     3601 ccattatgtg ctgaggaagc tggcggcctg agcggcggag ggaggactcg atgatcacat
     3661 ggctgctggc ggcgatggct ccggccgggc cgggctacga gccgccgccg caggtcttct
     3721 acccgcaggc ggtggcctgc gcggtctcgg ccaagctcgc cgccggcaag gcgatggacg
     3781 gcgaccggtt cggcgagctg atgacctggg gaatgatcct cgccgacgtc ggccgcaagg
     3841 ccggccggac gaaggcgcag gtcgacagcg ccgacctcgt cgccgccgag cccttctaca
     3901 agcacctcaa ggagaagaag cccaaggcct tcgccgccca ccgcgcctat tgccgggccc
     3961 tgctcgatgc tgatcgacct tagccacgcc atcgaggacg gcatggtcac ctatcggggc
     4021 ctgcccgcgc cgcacatttg cgactattgg aagcgcgagg acagcgccgc caattacgac
     4081 gacggctcga ccttccagat cggccggatc gacatggtcg ccaacaccgg cacctatctc
     4141 gacacgccct tccaccgcta cgccgacggc gacgacctcg ccgcggtcgg cctcgagcgc
     4201 ctggccgggc tcgacggcct gtgcgtgccc gccgagggca tggaagccgg agcggagctg
     4261 ttcgagggcc tcgacgtcgc cggcaaggcg gtgctggtcc ggaccggctg ggaccgccac
     4321 tggcggaccg acgcctattt ctccgaccac ccctatctgt cggagggcgc ggcacgcctg
     4381 ctggccgagc gcggcgccgc cctggtcggg atcgacagct acaatatcga cgacacgcgc
     4441 acccgccgcc ggcccatcca caccgtcctg ctcggcgccg gcaccctgat cgtcgagcat
     4501 atgaccaatc tcgccgcgct tccgccgagc ggcttccgat tcaccgcggc gccgccgaag
     4561 gtggcgggaa tgggcacctt cccggtgcgc gccttcgccg agatcggctg aagcacgttc
     4621 acggcggcga cagtttcggc ggcctagttt cgccggcgaa aggagagcgc ccatgctcca
     4681 tttcacgctc gccgcgctgc tcgccgccgc cgctcccgcc aacccgcaga tcgactatcc
     4741 gggcttccgc gagctggcgc tcgacgtcga ggactatcgc cgcagccgcc tcgtcgactg
     4801 gaagggcttc accgccgcgg cggccgagcc ggacgcgctg atcctcgacg cccgctccgc
     4861 ttcggccttc gcccgcggcc atatcgaggg cgcggtcaac cttcccttca ccgacttcac
     4921 cgatgaaagc ctcgccgccg tcatcggcag gagggaccgc ccgatcctca tctactgcaa
     4981 caacaatttc cgtaacgacg cgccgccggt ccagctcaag tcggtccggc ttgcgctcaa
     5041 tatccagacc ttcatcaacc tcgtcggcta cggctatgcc aacgtgcgcg agctgaacga
     5101 ggtgatcgac ttcaacgatc ccaaggtcag gtgggtgaag ggctgagcgc cgccgccagc
     5161 caaggcggaa cgatcgcgcc gccgagatcc tcgacccggc gatgctcgac cgagaggccg
     5221 agccccggat aatggacccg cgcccgctcc ccggggccct ccgccggcac gaccacgagc
     5281 cgccggttga ccttcgagcg gtagttcatc cgcgccgtat tctcctggcc gacatagcag
     5341 cccttggtga agctgactcc gtcgagctcg gcggcgttgc attcgagcca gagggtcttg
     5401 tcggagccca gctcggcccg gccctcggtc acccccagcg ccagccggtg ggcgagccaa
     5461 tcctgtccgc cggctccgtt ctcggcggcg agccagcgat gcccgagctc ggcgaggcgc
     5521 gggtcggcgg gtcccgggcc ttccggggcc caatggacgc ggagcaccgg gtcgggctcg
     5581 atcgcgatct tccgccgcag ccggtagagc gtcagccgct tcgccagatc gcccgccgcc
     5641 tcggcttcgc agtcgatcag gacgtcgccg ccgtccgccc acaggatgaa gtcgaacagg
     5701 gccttgccct gcggcgtgag cagccccgcc cacagcggcc ggtccggcgc cagcagggcg
     5761 aggtcgtggg tgaccagccc ctggaggaag ccgagcacgt cctcgcccga gaggcgaagc
     5821 accgcgcgat cgatgagggt ggtcgccgcc atcccccctt gatagggaca ccgccagcga
     5881 aacccaaggc gacatgaccg aaccgacccg cctcatcctg cgccgtcccg acgattggca
     5941 cgtccatctc cgcgacggcg acatgctcgc cgccgtcgct ccctccacgg cgcggcagtt
     6001 cgcccgggcg atcgtcatgc ccaatctcga tccgccggtg acgacggtgg ccgcggcgcg
     6061 ggcctatcgc gaccgcattc tcgaggcggt cggcagggcc cggttcacgc ccctgatgac
     6121 atgctacctg accgacgagg ccgatccggc cgagctcgcg tccggcttcg aggccggcgt
     6181 gttcgccgcg gccaagctct atcccgcgca tgcgaccacc aactcggctc acggcgtgac
     6241 cgccgtcgac cgcatccatc gggcgctcga ggcgatggag cgcgtcggca tgccgctgct
     6301 catccatggc gaggtcgtcg atccggacgt cgacatcttc gatcgggaaa ggctcttcat
     6361 cgaccgcgtc ttctcgcggc tggtgcggga cttccccgcg ctgaagatgg tgttcgagca
     6421 catcaccacc gccgatgcgg cggacttcgt gctggcggcc ggcccgaacg tcgccgcgac
     6481 ggtgactccg caccacctcc acatcaaccg caacgccttg ttcgccggcg ggctgaggcc
     6541 ccatgcctac tgccttccgg tcgccaagcg cgaggcgcac cggatcgccg tccggcgcgc
     6601 ggcgacctcg ggatcgcccc aattcttcct cggcaccgac agcgcgccgc acgcggccga
     6661 gcgcaaggag tccgactgcg gctgcgccgg gatcttcaac gcgccgttcg cgctggaaag
     6721 ctatgccgac gccttcgagg aggagggcgc gctcgaacgg ctcgaagcct tctcgtcgga
     6781 aaacggaccg cgtttctacg ggctgccg
//
LOCUS       DASVQZ010000422         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_186596, whole genome shotgun sequence.
ACCESSION   DASVQZ010000422 DASVQZ010000000
VERSION     DASVQZ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186596"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VF547_11925"
     CDS             complement(<1..809)
                     /locus_tag="VF547_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8263572.1"
                     /translation="MKISFASERPAGAYALAIPVWSEDMIADRLAGIDAGARIVAARA
                     AESQRFEREAASLAETFVAEGEGARRLLLVGLGDRRQDEALYEKVGGALVAKLLTSGE
                     TRLVIDVSGAGLDSEAAARIGFGAAARSWRYDLYRTKLAKKQKPTLEEVVIVGAADGA
                     ADSWSRREALLEGLSLTRELVTEPANIVYPETFVERCRKRLEPLGVEFEVLDEERMGE
                     LGMGALLGVGQGSIRPSRLLAMRWNGGEAGGKPVVFVGKGITFDTGGISIKP"
     gene            955..>2030
                     /locus_tag="VF547_11930"
     CDS             955..>2030
                     /locus_tag="VF547_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEX8263573.1"
                     /translation="MKKLNSICLTALPLLLAAPGSALAQQAAPRVDEGSAEAPAEPRD
                     VAFSADQLSYDSEAELVTATGDVRMTSEGNRLRAARVVWNRATDEVRAEGDVRVVNPG
                     GDVAYADSVVLTDALRDGIVQNMLLVLADGGRLAADRAERRDGFTTLYRAAYSPCAVV
                     SEKGCPKNPTWQINAVKVVHDPVRNRIRYEGASLNLLGARIVALPWLSHPDGTEGGGS
                     GLLVPEARLSSSNGFELSAPYYLKLAPNRDATITPHVYTEVAPMLEGSYRQLTRLGAF
                     QLGAYLTYGSRIPISTPAIAVRDEGIRAYLEGNGRFQLNPLWSVTASARYVTDRTFLR
                     RYDISRDSRLRSVVEAERIDENSY"
BASE COUNT          293 a          755 c          680 g          302 t
ORIGIN      
        1 ggcttgatcg agattccgcc ggtgtcgaag gtgatgccct tgccgacgaa cacgaccggc
       61 ttgccgcccg cctcgccgcc gttccagcgc atggccagaa gccgcgacgg ccggatcgag
      121 ccctggccga cgccgagcag ggcgcccatg ccgagctccc ccatccgctc ctcgtcgagc
      181 acctcgaact ccaccccgag cggctcgagc ctcttgcggc accgttcgac gaaggtctcg
      241 ggatagacga tgttcgccgg ctcggtgacc agctcccggg tcagggacag gccctcgagc
      301 agcgcctcgc ggcggctcca ggagtcggcg gcgccgtccg ccgcgccgac gatcacgacc
      361 tcctcgaggg tcggcttctg cttcttggcg agcttcgttc ggtagaggtc gtagcgccag
      421 ctccgcgccg cggcgccgaa gccgatccgg gccgcggctt ccgaatcgag cccggccccg
      481 gagacgtcga tcacgagccg ggtctcgccc gaggtcagca gcttggcgac gagggctccg
      541 ccgaccttct cgtagagggc ctcgtcctgc cggcggtcgc cgaggccgac gaggagcagc
      601 cggcgggcgc cctccccttc cgcgacgaac gtctcggcga ggctggccgc ttcgcgctcg
      661 aagcgctgcg actcggcggc gcgcgcggcg acgatgcggg cgcccgcgtc gatgccggcg
      721 agccggtcgg cgatcatgtc ctcgctccac accgggatgg cgagcgcgta ggcgccggcg
      781 ggacgttcac tggcgaagct gatcttcata agcgctgctg actccatggc tttggcttcc
      841 gcctctaggc cctcgccccg ccgctgcaaa ggggccggct tgcggcattg cagcgccgcc
      901 cgtccaatgc gataggctcg gaggcggtcg cgcgtgctcc tggcggcggg gaaagtgaag
      961 aagctgaatt ccatctgcct gaccgcgctg ccgctgctgc tcgccgctcc cggatcggcg
     1021 ctcgcccagc aggccgcgcc tcgggtcgac gagggctcgg ccgaagcccc ggctgagcct
     1081 cgcgacgtcg cgttcagcgc cgaccagctg tcctacgaca gcgaagccga gctggtgacc
     1141 gcgacgggcg acgtccgcat gaccagcgag ggtaaccggc tccgggccgc gcgggtggtc
     1201 tggaaccgcg ccaccgacga ggtccgcgcc gagggcgatg tccgagtcgt caacccgggc
     1261 ggcgacgtgg cctatgccga cagcgtcgtc ctcaccgacg cccttcgcga cggaatcgtc
     1321 cagaacatgc tgctcgtgct ggccgacgga ggccgcctcg ccgccgatcg ggccgagcgt
     1381 cgggacggct tcacgacgct ctaccgcgcc gcttattccc cctgcgcggt ggtcagcgag
     1441 aagggctgcc ccaagaaccc gacctggcag atcaacgcgg tcaaggtcgt ccacgatccg
     1501 gtcaggaacc ggatccgcta cgagggcgcg tcgctcaacc tgctcggcgc ccggatcgtc
     1561 gccttgccct ggctgtcgca ccccgacggc accgagggcg gcggaagcgg cctgctcgtc
     1621 ccggaggcgc ggttgagctc gagcaacggc ttcgagctgt ccgcgcctta ttatctgaag
     1681 ctggccccca atcgcgacgc gacgatcacc ccgcatgtct acaccgaggt cgctccgatg
     1741 ctggagggga gctaccggca gctgacccgc ctcggcgcct tccagctcgg cgcctatctg
     1801 acctacggct cgaggatccc gatcagcacg cccgcgatcg ccgtccgcga cgagggcatc
     1861 cgggcctatc tcgaaggcaa tggccgcttc cagctcaacc cgttgtggag cgtcaccgcc
     1921 tcggcccgct acgtcaccga ccgcacgttc ctccgccgct acgacatttc ccgcgacagc
     1981 cgcctgcgct ccgtcgtgga ggccgagcgg atcgatgaaa acagctacat
//
LOCUS       DASVQZ010000423         5081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_763821, whole genome shotgun sequence.
ACCESSION   DASVQZ010000423 DASVQZ010000000
VERSION     DASVQZ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763821"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..871
                     /locus_tag="VF547_11935"
     CDS             224..871
                     /locus_tag="VF547_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX8263574.1"
                     /translation="MIRLFRAAGFAAAAFVAVFGAGYAGPSLAWDLTGTSLTDPISDQ
                     KPVDALGAPPPAAAAPAAPAPAEDAPAEPAEKPARLSLAELVAAHADSRTADAEHDCL
                     AGAVYFESKGEPLHGQLSVAEVILNRARSGRFPSSVCGVVKQRGQFSFVRGGRIPAIA
                     RASQAWKRAVAIAHIAREQLADGAAPRALFFHARRVSPRWKLTRVASVGNHIFYR"
     gene            910..1407
                     /locus_tag="VF547_11940"
     CDS             910..1407
                     /locus_tag="VF547_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX8263575.1"
                     /translation="MAARPSLQPELCFADAPPAARDVARGVTRLFFNRDLFALCEVPL
                     PNGRRADMMAIDSAGLITIVEIKVSRADLMGDGKWIEYLAYCDRFFWAVPASFDLTPF
                     DEPGFQPQLCGLLVADRYDAAVVREAPLRKLPPARRKAETLRFARRAARRLVGDLDPG
                     LAGFA"
     gene            complement(1507..2112)
                     /locus_tag="VF547_11945"
     CDS             complement(1507..2112)
                     /locus_tag="VF547_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX8263576.1"
                     /translation="MHNKFGRALTALLVLILAAVPASAQNFSDSYSFLKAVKERDGAK
                     VNELVGGTTGAIVVNSRDRTNGEGALHYMVRARDITWLAFLLGKGARPDMQSNRGDTP
                     LTLAAQIGWLEGAEQLLARRASVDLANGRGETPLILATQRRDLPMVRLLLSRRADPNR
                     TDNVAGMSALDYARQDPRAGAVLKLLEAKLAPAKPAAGPTK"
     gene            2172..2771
                     /locus_tag="VF547_11950"
     CDS             2172..2771
                     /locus_tag="VF547_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8263577.1"
                     /translation="MNETSAPRLVTILCLLLGLGLAACSGGGEEPPLKGAAIGGPFAL
                     VDQDGRATTDRDFAGRYRIMYFGFTHCPDICPTDLAVLGQALRRFETSDPARAARVTP
                     IFVSVDPERDSPAVLKEYVAAFHPRLVGLTGTPRQLADMSRRYGAYYGKGAPTEGSGY
                     DVNHSRLAELIGPQGEPIALLPYDKGAEAVAAELDRWVR"
     gene            2768..3193
                     /locus_tag="VF547_11955"
     CDS             2768..3193
                     /locus_tag="VF547_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX8263578.1"
                     /translation="MSFWEKPLAALSRAEWEALCDGCGKCCLHKVEDDETGQIYPTNV
                     ACRLLDRHTARCTNYRGRKAFVPDCIRLTAPRVAAYAWLPSTCAYRLRAEGKPLPEWH
                     YLVCGDREAVHRAGMSIRGWTVSEDEAGGLDHHLVEREL"
     gene            3183..3857
                     /locus_tag="VF547_11960"
     CDS             3183..3857
                     /locus_tag="VF547_11960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX8263579.1"
                     /translation="MSFELRLAVSPRARTIRLRVDRRTGEVVLTIPKRASRRKALAWA
                     DGHRAWIEAQLAKVAPAVPLVPGASVPLYDRPHRIDWSPDRPRTPRLEEGRLVVGGPL
                     DGVEPRLLRWLRRHALDLLTAETAEFAAAAGVKVARVGVGDPLSRWGSCSAAGGIRYS
                     WRLVLAPDWVRRATVAHEVAHRVHMNHGPDFHRLVEQLLGSDPKPARLWLRRHGPFLH
                     RVGLAV"
     gene            complement(3804..>5081)
                     /locus_tag="VF547_11965"
     CDS             complement(3804..>5081)
                     /locus_tag="VF547_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8263580.1"
                     /translation="PQLDTLIDETVEPIEVWTTLDPNMQRLGDQVINAATPAGAQGAL
                     VALDRDGSVRAMIGGRDYVRSLYNRATQANRQPGSSFKLFVYLAAIEAGYKPDDLVSD
                     EPLTINGWSPRNNNRGFAGQVTIREAFAKSINTVSVRLAQEVGFRTVADMAQRFGITT
                     PVVTHPSMALGSSEVRLLDMTRAFAAVAAKGNAVVPYGIRRVTTADGAILYQHPPAES
                     RVLVAPWVAAQMTDLLQAAVLTGTGRGAQIGRPVAGKTGTTSSNKDGWFLGFSSGLTT
                     GIWMGRDDNKAIPGLQGGRAPARAFHDFMARAVANRPAEPFEILVQTPEWAITEPDEE
                     TWFEAPDNGVFVNPDGSGPAGPIPPPPAREEDERPLPDEDQDRLDQDFIDRATDRVRP
                     EPPRRRDRDAPRPERPSDREADPVEKRPMTAEP"
BASE COUNT          657 a         1816 c         1811 g          797 t
ORIGIN      
        1 tcccgtctgc cgaacggttc gccgctggaa tgaacgcatt cgattgactc tgctgcagcg
       61 cacaacctag cccgcgctca gccctttcga gcaagagcgg ggggccgtca tgatcccggg
      121 gtattggggg cgggcggccg cgcgagacgg aaggcagtcc gggatgcgct tctcttccta
      181 tgcatccggc tttggaccgc ccgccgcatt gatttgagat ttgatgattc gtctgtttcg
      241 cgccgcgggt ttcgcggctg ccgctttcgt tgccgtcttc ggcgccggct atgcgggacc
      301 ctcgctggcc tgggacctga cgggaacctc cctcaccgac ccgatttccg accagaagcc
      361 cgtggacgcg ctcggcgcgc cgccgcccgc cgccgccgct ccggccgcgc ccgctcccgc
      421 cgaggatgct ccggccgagc ccgccgagaa gcccgcgcga ctcagccttg ccgagctggt
      481 cgccgcccat gccgacagcc gcacggcgga cgccgagcac gactgcctcg cgggggccgt
      541 ctatttcgag tccaagggcg agcctctcca cggccagctc agcgtcgccg aagtgatcct
      601 caaccgcgcg cgctccggcc gctttccctc gtcggtctgc ggggtggtca agcagcgcgg
      661 ccagttctcc ttcgtccgcg gcgggcgcat ccccgccatc gcccgcgcct cgcaggcgtg
      721 gaagcgggcc gtcgccatcg cccatatcgc ccgcgagcag ctggccgacg gcgcggcgcc
      781 gcgggccttg ttcttccacg ctcgccgggt gtcgccgcgc tggaagctga cccgcgtggc
      841 gtcggtcggg aatcacatat tctaccgttg aggcttttcc ccgttcccga tccattctag
      901 cctggccgga tggccgcccg tccgagtctc cagcccgagc tttgcttcgc cgacgcaccg
      961 cccgccgccc gggacgtggc gcgcggagta acccggctgt tcttcaaccg ggacctgttc
     1021 gccctgtgcg aggtgccgct gcccaacggc cggcgcgccg acatgatggc gatcgactcg
     1081 gcaggcctga tcaccatcgt cgagatcaag gtgtcgcgcg ccgacctcat gggcgacggc
     1141 aagtggatcg aatatctcgc ctattgcgac cgtttcttct gggccgtgcc ggccagcttc
     1201 gacctcactc cgttcgacga gccgggcttc cagccgcagc tgtgcggcct gctcgtcgcc
     1261 gaccgctacg acgcggccgt cgtccgcgag gcgccgttga ggaagcttcc gccggctcgg
     1321 cgcaaggccg agactctgcg tttcgcgagg cgggcggcgc ggaggctggt gggggacctc
     1381 gatccggggc tggccggctt cgcttaggcg gccgtcagtg caagcgcagc gcaaggagtc
     1441 cggtttcgac cggtcgaagg ccgcgagctg gattgccgcg tcgccgcgcg cggcgcagcg
     1501 acgaggctat ttcgtcggcc ccgcggccgg cttggcgggc gccagcttcg cctcgagcag
     1561 cttgagaacc gcgccggcgc gcggatcctg ccgggcgtag tcgagcgccg acattccggc
     1621 gacgttgtcg gtgcgattcg ggtcggctcg gcgcgacagc agcagccgta ccatcggcag
     1681 gtcgcggcgc tgggtggcga ggatgagcgg cgtttccccg cgcccgttgg cgaggtcgac
     1741 gctcgcccgg cgtgcgagca attgctccgc gccctcgagc cagccgatct gggcggcgag
     1801 tgtgagcggc gtgtcgccgc ggttgctctg catatccggc cgggcgccct tgccgagcag
     1861 gaaggccagc caggtgatgt cgcgcgctcg gaccatgtag tggagcgcgc cctcgccgtt
     1921 cgtgcgatcg cgggaattga cgacgatcgc gcccgtcgtg ccgccgacaa gctcgttgac
     1981 cttggcgccg tctcgctcct tcaccgcctt gaggaaggag tagctgtccg aaaagttctg
     2041 cgccgaggcg gggaccgcag ccaggatcag gacgagcaac gccgtcaacg cgcggccgaa
     2101 cttgttatgc actctcgctt ccccgtctcg acttgatcgc gcgcctatag caagacaagc
     2161 ctgcctccgc catgaacgaa acttccgcgc cccgcctcgt gacgatccta tgcctcctgc
     2221 tcgggctggg ccttgccgcc tgctcgggcg ggggcgagga gccgccgctc aagggagcgg
     2281 cgatcggcgg cccgttcgcg ctcgtcgacc aggacggccg ggcgacgacc gaccgcgatt
     2341 tcgccggccg ctaccgaatc atgtatttcg gcttcaccca ttgccccgac atctgcccga
     2401 ccgacctcgc cgtcctcggc caggcgcttc gccggttcga gacgagcgat cccgcgcgcg
     2461 cggcgcgggt gaccccgatc ttcgtcagcg tcgaccccga gcgcgactcc cccgcggtgc
     2521 tcaaggaata tgtcgccgcc ttccaccccc gcctggtcgg cctcaccggc acgccccggc
     2581 agctcgccga catgagccgc cgctacgggg cctattatgg caagggggcc ccgaccgagg
     2641 ggagcgggta cgacgtcaac cacagccgtc tcgccgagct gatcggcccg cagggagagc
     2701 cgatcgccct cctgccgtac gacaaggggg ccgaggcggt ggccgccgag ctcgaccggt
     2761 gggtgcgatg agtttctggg aaaagccgct cgccgcgctc agccgcgccg aatgggaggc
     2821 gctctgcgac ggctgcggca aatgctgcct tcacaaggtg gaggacgacg agacggggca
     2881 gatatatccg accaacgtcg cctgccggct gctcgaccgc cacaccgcgc gatgcaccaa
     2941 ttatcgcggc cgcaaggcct tcgtccccga ctgcatccgc ctgaccgccc cccgcgtcgc
     3001 ggcctacgca tggcttccct cgacctgcgc ctatcggctg cgcgccgagg gcaagccgct
     3061 gccggaatgg cattacctcg tctgcggaga ccgcgaggcc gtccaccgcg cgggcatgtc
     3121 gatccgcggc tggacggtga gcgaggacga ggccggcggc ctcgaccacc atctggtcga
     3181 acgtgagctt tgagctgagg ctcgccgtgt cgccgcgcgc gcgcacgatt cggctgcggg
     3241 tcgaccggcg cacgggcgag gtcgtgctca ccatcccgaa gcgcgcgtcg cggcgcaagg
     3301 cgctcgcctg ggccgacggc caccgcgcct ggatcgaggc gcagctggcg aaggttgcgc
     3361 cggccgtgcc gctcgtcccg ggcgcgagcg tgccgctcta cgaccggccg caccggatcg
     3421 actggagccc cgaccggccg cgcacgccgc ggctggagga gggaaggctg gtcgtgggcg
     3481 gcccgctcga cggggtcgag ccccggctgc tgcgctggct gcgccgccac gccctcgacc
     3541 tgctcaccgc ggaaaccgcc gaattcgccg cggccgcggg cgtgaaggtg gcccgggtcg
     3601 gagtcggcga cccgctgtcg cgctggggca gctgctcggc cgcgggcggg atccgctaca
     3661 gctggcggct cgtcctcgcg ccggactggg tgaggcgggc gacggtcgcc catgaggtgg
     3721 cgcaccgagt ccacatgaat cacgggccgg atttccaccg gctggtcgag cagctcctcg
     3781 gctcggaccc gaagccggcg cggctatggc tccgccgtca tgggccgttt ctccaccggg
     3841 tcggcctcgc ggtctgacgg gcgctcgggg cgcggggcgt cgcggtcccg gcgccgcggc
     3901 ggctcgggac gaacgcgatc ggtcgcgcgg tcgatgaagt cctggtcgag gcggtcctgg
     3961 tcctcgtcgg gcaggggccg ctcgtcctcc tcgcgggccg gcggcggcgg gatcggaccc
     4021 gccgggccgc tgccgtcggg attgacgaac acgccgttgt ccggcgcctc gaaccaggtc
     4081 tcctcgtcgg gttcggtgat cgcccattcg ggagtctgga ccaggatctc gaacggctcg
     4141 gccggccggt tggcgacggc tcgggccatg aagtcgtgga acgcgcgcgc cggcgcccgg
     4201 ccgccctgga ggccgggaat cgccttgttg tcgtcgcggc ccatccagat tccggtggtg
     4261 aggccgctcg agaagcccag gaaccagccg tccttgttcg agcttgtggt ccccgtcttg
     4321 ccggcgaccg gccggccgat ctgggcgccg cggccggtgc cggtcagcac cgccgcctgg
     4381 agcaggtccg tcatctgggc ggcgacccag ggcgcgacca gcacccggct ctcggccggc
     4441 ggatgctggt agaggatggc gccgtcggcg gtggtgaccc gcctgatccc gtaggggacc
     4501 accgcattgc ccttggcggc gaccgcggcg aaggcccggg tcatgtcgag cagccggacc
     4561 tcggagctgc ccagcgccat cgagggatgg gtgacgaccg gggtggtgat cccgaagcgc
     4621 tgggccatgt cggccacggt ccggaacccc acctcctggg cgagccgcac cgagacggtg
     4681 ttgatcgact tggcgaaggc ctcgcggatc gtgacctgcc cggcgaagcc gcggttgttg
     4741 ttgcgcgggc tccagccgtt gatcgtgagc ggctcgtcgg agacgaggtc gtcgggcttg
     4801 tagccggctt cgatcgcggc caggtagacg aacagcttga acgacgagcc gggctggcga
     4861 ttggcctggg tggcgcggtt gtagagcgat cgcacatagt cgcgcccgcc gatcatcgcc
     4921 cgcaccgagc cgtcccggtc gagcgcgacc agagcgccct gggcgccggc gggcgtcgcg
     4981 gcgttgatca cctggtcgcc gagccgctgc atgttgggat cgagcgtcgt ccagacctcg
     5041 atcggctcga ccgtctcgtc gatcagggtg tcgagctggg g
//
LOCUS       DASVQZ010000424         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_665557, whole genome shotgun sequence.
ACCESSION   DASVQZ010000424 DASVQZ010000000
VERSION     DASVQZ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665557"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF547_11970"
     CDS             <1..327
                     /locus_tag="VF547_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX8263581.1"
                     /translation="DALAEAPIAAVVGEGVVVSGSVDRLLVRPDRILVADFKTGRRAP
                     ATLDEIPVAHLRQMAAYVEALGTIFPGRRIEAKLLYTAAPVLFDLPAALLERHRPTLE
                     PAGPAP"
     gene            358..678
                     /gene="trxA"
                     /locus_tag="VF547_11975"
     CDS             358..678
                     /gene="trxA"
                     /locus_tag="VF547_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX8263582.1"
                     /translation="MSTKTVTDASFQNDVLGSDKPVLVDFWAEWCGPCRMIAPALEEI
                     SNELGEKVTVAKLNIDDNPDAPARYGVRGIPTMILFKGGQPAATKVGAAPKNQLQSWL
                     EQVL"
     gene            complement(806..1252)
                     /locus_tag="VF547_11980"
     CDS             complement(806..1252)
                     /locus_tag="VF547_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8263583.1"
                     /translation="MAEGLEGGCACGAVRYRLESEPFDAGWCHCRTCQLNSGAPAMVF
                     ASVPAGDLVWTGGADKVKSFKSSGFGHRTFCGECGTPFLMEVDHQPETVDFSVATLDS
                     PERAAPGFHIFWASRIRWFEPGDDLPRHARFRPDTRGLEGTEPPGD"
     gene            1329..1655
                     /locus_tag="VF547_11985"
     CDS             1329..1655
                     /locus_tag="VF547_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263584.1"
                     /translation="MSHDAALASERARWIGLLELLLFLSAMLAGLTGLISGDRAVDAR
                     QVERTAVAASTAADAGVRIAEASSVAEAAYPLAQPSARPASDDRRAATDEARPQAAPV
                     DERRLE"
     gene            1759..>2296
                     /locus_tag="VF547_11990"
     CDS             1759..>2296
                     /locus_tag="VF547_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX8263585.1"
                     /translation="MLGGLAKAIFGSSNDRYVRSLGKIVDKINGFEPTLAAMTDDELA
                     NQTVLFRRRLAEGEELDDLLPEAFATVREAARRTLGQRHYDVQMIGGIVLHRGEIAEM
                     RTGEGKTLVATLAVYLNALEGKGVHVVTVNDYLARRDADWMGQIYRFLGMTVGVIVPN
                     LTDYQRRQAYGSDITYGTN"
BASE COUNT          369 a          841 c          755 g          331 t
ORIGIN      
        1 gacgccctcg ccgaggcgcc gatcgcggcg gtggtggggg agggcgtggt cgtctcgggc
       61 tcggtcgacc gcctgctggt ccgccccgac cggatcctcg tcgccgattt caagaccggg
      121 cgccgagccc ccgccacgct cgacgagatc cccgtcgccc accttcgcca gatggccgcc
      181 tatgtcgagg ccctgggcac catcttcccg ggccggcgga tcgaggccaa attgctctac
      241 accgccgcgc cggtcctgtt cgacctgccg gcggcgctgc tcgagcgtca tcggccgacg
      301 cttgagccgg cgggtccggc tccctaaatg cgtgccgacc ccccccagga gaaagccatg
      361 agcaccaaga ccgtcaccga cgccagcttc cagaacgacg tcctcggctc ggacaagccc
      421 gtcctcgtcg atttctgggc ggaatggtgc ggcccctgcc ggatgatcgc cccggcgctc
      481 gaggagatct cgaacgagct gggcgagaag gtcaccgtgg ccaagctcaa catcgacgac
      541 aatcccgacg cgccggcccg ctacggcgtc cgcgggatcc cgacgatgat cctgttcaag
      601 ggcggccagc cggccgccac caaggtcggc gcggcgccca agaaccagct gcagagctgg
      661 ctcgagcagg tgctgtaatc ctccccatcg aagacgggga gtgggaccac ccctagggtg
      721 gcggaggggt aattgacgag gtctcagcaa ttacccctcc accatgctgc gcatggtccc
      781 cctccccgag ccagctcggg gagggctagt cgccgggcgg ctcggtcccc tcgagccccc
      841 gtgtgtccgg cctgaaccgg gcgtggcgcg gcaaatcgtc gcccggctcg aaccagcgga
      901 ttcggctcgc ccagaagatg tggaagcccg gcgccgcccg ctcgggcgaa tcgagggtgg
      961 cgacgctgaa atcgaccgtc tcgggctggt gatcgacctc catcaggaag ggcgtgccgc
     1021 attcgccgca gaaggtccga tggccgaagc ccgaggactt gaacgacttc accttgtcgg
     1081 cccccccggt ccagacgagg tcgccggccg ggacgctggc aaacaccatc gccggcgctc
     1141 ccgagttgag ctggcaggtg cggcagtggc accagccggc gtcgaacggc tcgctttcga
     1201 gccggtagcg gaccgcgccg caggcgcagc ctccttcgag tccctcggcc atgggcgcgc
     1261 cgctagtctt tcgcggtcgt ggaggtcaat cctcggctcg cttatggacg gcggcggccg
     1321 caatcactat gtcgcatgac gcggcgctcg ccagtgagcg cgcgcgctgg atcgggctgt
     1381 tggaacttct gctcttcctc tcggcgatgc tggccggcct caccggattg atttccggcg
     1441 accgggccgt cgacgcgcgc caggtcgagc gaaccgcggt ggcggccagc acggcggccg
     1501 atgccggcgt ccgcatcgcc gaagcgagca gcgtcgccga agccgcctat cccctcgcgc
     1561 agccctcggc ccggccggct tcggacgacc gccgcgccgc caccgacgag gcccggcccc
     1621 aggccgcccc ggtcgacgag cgccggctgg aatagccgtt cgggacccgc ccgagcgcgg
     1681 ggacgttgca ctcccgacgc aatcaggcgc gctcaggacg gcgcgccctc gcccaaccag
     1741 atacgacagg aatttgctat gctcggcgga ctcgccaagg ccattttcgg atcgtcgaac
     1801 gaccgctacg tgcgctcgct cggcaagatc gtcgacaaga tcaacggatt cgagccgacc
     1861 ctcgcggcga tgaccgacga cgagctcgcc aaccagaccg tcctgttccg gcgccgactg
     1921 gcggagggcg aggagctcga cgatctcctc cccgaagcct tcgccaccgt ccgcgaggcg
     1981 gcccggcgga cgctcggcca gcgccattac gacgtccaga tgatcggcgg aatcgtcctc
     2041 catcgcggcg agatcgccga gatgcgcacc ggcgagggca agacgctggt cgccacgctc
     2101 gccgtctatc tgaacgcgct ggagggcaag ggcgtccacg tcgtcaccgt caacgactat
     2161 ctcgcccgcc gcgacgccga ctggatgggc cagatctacc ggttcctggg aatgaccgtc
     2221 ggagtcatcg tcccgaacct caccgactat caacgccgcc aggcctatgg ctcggacatc
     2281 acctacggca ccaaca
//
LOCUS       DASVQZ010000425         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_665577, whole genome shotgun sequence.
ACCESSION   DASVQZ010000425 DASVQZ010000000
VERSION     DASVQZ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665577"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..793)
                     /locus_tag="VF547_11995"
     CDS             complement(113..793)
                     /locus_tag="VF547_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8263586.1"
                     /translation="MLSMRELSRVYRADTVETTALDRIDLDIAEGEFVAIMGPSGCGK
                     STLLNVVGMLDSPSSGSYVFDGTEVAGLPESKLADFRKKNIGFIFQSFNLVDELSVRE
                     NIELALLYHDVPAAERRPRVEAVMDTVGVAHRAKHRPSQLSGGQQQRVAVARALVGNP
                     KLILADEPTGNLDTQHGEEVMKMLQALNREGSTIVMVTHSPAHADYAGRVVNMLDGRI
                     LIERRRAA"
     gene            complement(809..2113)
                     /locus_tag="VF547_12000"
     CDS             complement(809..2113)
                     /locus_tag="VF547_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8263587.1"
                     /translation="MIRIKSTESESRAESGTGMDKVVVATGLTKQMKIALGAAAVLLL
                     AFLFWWFAPSAASQTVAAERLTVSAVSRGTFDDFLPLRARVEPLVTVFLDAVEGGRVE
                     KIMVEDGAMVQQGQLLAVLSNSDLALNLLARQSDVSREVNSMRSQELALAQTSIGDER
                     AVIDAQLAADKARRQYEMQKPLMEKGYVPAKVFNDSRDEYLSARRRADVLKRAREVNQ
                     RLQTSQLAQLRASNASLSGSLDIARSTLDALNLRAPVSGQLTAFSIQVGQSLNRGERL
                     GQVDSAGRNKLVASVDEYYLGRVQPGQTATVESGGKTYRVKVAKIYPQVKNGVFEADL
                     YFVGAEPPSLNRGQTLQTKLTLGDPTPALLIPNGAFYNETGGAWVFVVAPGGGEAVKR
                     NVRLGRRNADYIEVLEGLEPGERVITSPYTGFADKDRLDLET"
     gene            complement(2174..2359)
                     /locus_tag="VF547_12005"
     CDS             complement(2174..2359)
                     /locus_tag="VF547_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263588.1"
                     /translation="MRKFETWLTGLLLIALALLLPMAALGPVSLANARASEGGLAAAA
                     CDDGSAHLVMGCASIAL"
BASE COUNT          409 a          898 c          885 g          456 t
ORIGIN      
        1 catgcgaagc atggtggagg ggccttgcgg actccgaaga acccctccac cgcgctgcgc
       61 gcggtccccc tcccccgcaa atgcggggga ggatttgaag aaaagacccc tctcacgccg
      121 cgcggcgccg ctctatgagg atccgcccgt cgagcatgtt cacgacccgc cccgcataat
      181 cggcatgggc cggcgagtgg gtgaccatga cgatggtcga gccttcgcgg ttcagcgcct
      241 ggagcatctt catcacttcc tcgccatgct gggtgtcgag gtttccggtc ggctcgtcgg
      301 cgaggatcag cttgggattg ccgacgaggg cccgggcgac cgcgacccgc tgctgctgtc
      361 cgccggaaag ctggctcggc cggtgcttgg cccggtgggc cactcccacc gtgtccatca
      421 ccgcctcgac gcgcgggcgc cgttcggccg cgggaacgtc gtgatagagc aaggcgagct
      481 cgatattctc gcggacgctg agctcgtcga ccaggttgaa gctctggaag atgaagccga
      541 tattcttctt ccggaagtcg gcgagcttgc tctcggggag gcccgccacc tcggtcccgt
      601 cgaagacgta ggagccgctg ctcgggctgt cgagcatccc cacgacgttg agcaaggtcg
      661 acttgccgca gccggaggga cccatgatcg ccacgaactc gccctcggcg atgtcgaggt
      721 cgatgcggtc gagcgccgtc gtctcgaccg tgtcggcgcg gtagacccgc gacagttcac
      781 gcatgctcag cataactttt ccgctccttc aggtctcgag gtccagccga tccttgtcgg
      841 cgaaaccggt ataaggtgag gtgatgaccc gctcgccggg ctccagcccc tcgagcactt
      901 cgatgtaatc ggcgttgcgg cggccgagcc ggacgttgcg cttcaccgct tcgccgccgc
      961 ccggcgcgac cacgaagacc caggctccgc cggtctcgtt gtagaaggcg ccgttgggga
     1021 tgagcagcgc cggggtcgga tcgccgagcg tcagcttggt ctggagggtc tggccgcggt
     1081 tgagcgacgg cggctccgcg ccgacgaaat agaggtcggc ctcgaacact ccgttcttca
     1141 cctgcgggta gatcttggcg accttgaccc gataggtctt gcctccggac tcgacggtcg
     1201 cggtctggcc cggctggacc cggccgagat aatattcgtc gacgctcgcg accagcttgt
     1261 tccggccggc gctgtcgacc tggccgagcc gctcgccgcg attgagcgac tggccgacct
     1321 ggatcgagaa ggcggtgagc tggccggaga ccggggcccg caggttgaga gcgtcgaggg
     1381 tcgagcgggc gatgtcgagg cttccgctca gcgaggcgtt ggacgcccgg agctgggcga
     1441 gctggctggt ctggaggcgc tggttgacct ccctggcgcg cttcagcacg tcggcgcggc
     1501 gccgggcgga gagatattcg tcgcggctgt cgttgaagac cttggcaggc acgtagccct
     1561 tttccatcag cggcttctgc atctcatact ggcgcctggc cttgtcggcc gcgagctggg
     1621 cgtcgatcac cgcgcgctcg tcgccgatgc tggtctgggc cagagccagc tcctggctcc
     1681 gcatcgagtt gacctcgcgc gacacgtcgg actggcgggc gagcaggttg agagcgaggt
     1741 ccgaattgga gaggacggcg agcagctggc cctgctggac catcgccccg tcctccacca
     1801 tgatcttctc gacccggccg ccctcgaccg cgtcgaggaa gacggtgacc agaggctcca
     1861 cccgggccct cagcggaagg aagtcgtcga acgtgccccg gctcaccgcc gacacggtca
     1921 gccgctcggc cgccaccgtc tggctggccg cgctcggcgc gaaccaccag aagagaaaag
     1981 cgaggaggag cacggccgcc gctcccaagg cgatcttcat ctgcttcgtc agaccggtgg
     2041 cgacgaccac cttgtccatt ccggtgccgc tttccgcccg cgattccgat tccgtggatt
     2101 tgatcctgat cacgctcatc ccaacctcat gccgtccgat atcggacaca accttgtacg
     2161 ctcacggaca ggatcacagg gcgatgctcg cgcagcccat caccaggtga gcgctcccgt
     2221 cgtcgcaggc ggcggccgcc aggccgccct ccgacgctcg ggcgttcgcc aggctcaccg
     2281 gcccgagcgc cgccatcgga agaagcaagg cgagggcgat gagcagcagc ccagtcagcc
     2341 aggtctcgaa tttgcgcatt gcgtggactc cccgatcttc cctgtcgccc tcttcccagc
     2401 atgaggcgtg ccaatcgcgg aaatcggcga ttcccggcga tttgcggcga tcgccggcgc
     2461 cgggccgcgt ccgcttccgg acgcgccgga gtccgtcccc ggacgcccag ccaaggccgc
     2521 ggcgccattg gcacgattat tgtttgtccc tccccggaca ggcgggtagg agggaagacg
     2581 tggctgggga gggaaagccg ttctggcgct gcatcgtcgt cgatgacgat cccgacatcc
     2641 tgctctcc
//
LOCUS       DASVQZ010000426         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_377561, whole genome shotgun sequence.
ACCESSION   DASVQZ010000426 DASVQZ010000000
VERSION     DASVQZ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377561"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VF547_12010"
     CDS             <1..776
                     /locus_tag="VF547_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8263589.1"
                     /translation="ALAVAQDRLSEKGFIAGLGGRPPAYRAVDDLAGLEAAIEALGLP
                     AVLKTRRFGYDGKGQARLAQASDAESAWTTVRGSPSILEAFVPFEAEFSILLCRGRDG
                     KTVVWPAPRNVHEGGVLRRSTVPAEGLEAQIAEGEALARRAADRLDYVGVLALEFFAA
                     PQGPLFNEMAPRVHNSGHWTIEGAVTSQFENHVRAICGLPLGSTALAGLRVEMDNLIG
                     GEADSWSEILSDPASHLHLYGKGEARPGRKMGHVTRLIR"
     gene            complement(865..>2307)
                     /locus_tag="VF547_12015"
     CDS             complement(865..>2307)
                     /locus_tag="VF547_12015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8263590.1"
                     /translation="PGERPMAPLPYVAPAADDAAERAPLSYQKIPMPEVGEISNLDFP
                     DIERATLSNGIPVVYARRTAVPVVRVAVEFDAGVAADPANALGTQSLMLSLLDEGTTT
                     RNSIQIAEEQERLGAVIGPDTSLDRTAVSMAALTPNLGPSLDLLADVIRNPAFAPGEV
                     ERLRAQQLAAIAQELTEPSGLGARTLPPLLFGPGHPYGRPSSGTGDKEVVARLTRDDL
                     IRFHQTWIRPDNATIFAVGDLPLAELVPMLEARFGSWTAPAVPKGTKNFAMTVPTPKP
                     RIVLVNRPQSPQSLILAGVVLPVRGTEDVLTLNAANEVLGGNFLSRINMDLRETKGWS
                     YGSRSNLGMREQLVPYMITAPVQADKTGPAIRALMDHVSSFVTTKGLTDVELTRVING
                     NTRQLAGQFETSQAVLGALRSNALYRRPDNYWEAIADRYRGMTISTIDQAARKVIDPK
                     KIVWVVVGDAAKVRPQLSGLGLPVEEVQPR"
BASE COUNT          336 a          774 c          840 g          357 t
ORIGIN      
        1 cggccctcgc cgtcgctcag gaccgactga gcgagaaggg cttcatcgcc gggctcggcg
       61 gccggcctcc cgcctatcga gcggtcgacg atctcgccgg cctcgaggcg gcgatcgagg
      121 cgctcggcct acccgccgtc ctgaagaccc gccgattcgg ctatgacggc aagggccagg
      181 cgcggctggc tcaagcctcg gatgccgagt cggcctggac gacggtgcgc gggtcgccct
      241 cgatcctcga ggccttcgtt ccgttcgagg ccgaattctc gatcctcctc tgccgcggcc
      301 gggacgggaa gacggtcgtc tggccggcgc cgcgcaacgt ccacgagggc ggcgtgctgc
      361 gccgctcgac ggtcccggcc gagggcctgg aggcgcagat cgccgaaggc gaggctcttg
      421 cacgtcgggc cgcggaccgg ctcgattacg tgggcgtgct cgcgctcgaa ttcttcgccg
      481 ctccgcaggg accgctattc aacgagatgg cgccgagggt ccacaatagc ggccattgga
      541 cgatcgaggg cgccgtcacc tcgcagttcg agaaccacgt gcgggcgata tgcggactgc
      601 cgctgggatc gaccgcgctc gccggcctcc gggtggagat ggacaatctg atcggcggcg
      661 aggcggacag ctggagcgag atcctgtcgg atccggcctc gcacctccat ctttacggca
      721 aaggcgaggc acggccgggc cggaagatgg ggcatgtaac caggctcatc cgctagccgt
      781 cgttgcgagg agcgaagcga cgaagcaatc cagcgggcgc ttacgccgca ctggattgct
      841 tcgctccgct cgcaatgacg agggctatct cggctgcacc tcctcgaccg gcaggccgag
      901 gccgctgagc tgcgggcgca ccttggcggc gtcgccgacc accacccaga cgatcttctt
      961 gggatcgatc accttgcgcg ccgcctggtc gatcgtgctg atcgtcatgc cgcggtagcg
     1021 gtcggcgatc gcctcccaat aattgtcggg gcgccggtag agggcgttgg agcgaagcgc
     1081 gccgagcacc gcctgcgacg tctcgaactg gccggcgagc tgacgggtgt tgccgttgat
     1141 cacccgcgtg agctcgacgt cggtcaggcc cttggtcgtg acgaagctcg acacatgatc
     1201 catcagcgcc cggatcgccg ggccggtctt gtcggcctgc acgggggcgg tgatcatgta
     1261 ggggacgagc tgctcacgca tgccgaggtt gctgcgcgac ccgtaggacc agcccttggt
     1321 ctcgcgcagg tccatgttga tgcgcgacag gaagttgccg cccagcacct cgttggccgc
     1381 gttgagggtg agcacgtcct cggtgccgcg caccggcagg accacgcccg ccaggatcag
     1441 cgactgcggc gactgcgggc gattgacgag gacgatgcgc ggcttgggag tcggcaccgt
     1501 catcgcgaag ttcttggtcc ccttcggcac cgccggggcg gtccagctgc cgaaccgcgc
     1561 ttcgagcatc ggcaccagct cggcgagcgg aaggtcgccg accgcgaaga tggtggcgtt
     1621 gtccggccgg atccaggtct ggtggaagcg gatgagatcg tcgcgggtga gccttgccac
     1681 cacctccttg tcgccggtgc cgctggacgg ccggccgtag gggtgccccg gtccgaacag
     1741 cagcggcggc agagtgcgcg cgccgagccc gctcggctcg gtcagctcct gcgcgatcgc
     1801 ggcgagctgc tgcgcccgaa gccgctccac ctcgcccggg gcgaaggccg ggttgcggat
     1861 gacgtcggcg agaagatcga gcgacggccc gagattgggc gtgagcgccg ccatgctgac
     1921 ggcggtacgg tcgagcgagg tgtccggccc gatcacggcg ccgagccgct cctgctcctc
     1981 ggcgatctgg atcgagttgc gggtcgtcgt gccctcgtca agcaggctca gcatcagcga
     2041 ctgcgtgccg agcgcattgg cgggatccgc ggcgacgccg gcgtcgaact cgaccgcgac
     2101 ccgcaccacc ggcacggccg tgcggcgggc gtagacgacc gggattccgt tcgacagggt
     2161 ggcgcgctcg atgtccggga agtcgaggtt cgaaatctcg ccgacctcgg gcatcgggat
     2221 cttctgatag gagagcggcg ccctctccgc ggcgtcgtcg gcggcgggcg cgacataggg
     2281 cagcggcgcc atcggccgct cgccggg
//
LOCUS       DASVQZ010000427         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_284510, whole genome shotgun sequence.
ACCESSION   DASVQZ010000427 DASVQZ010000000
VERSION     DASVQZ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284510"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VF547_12020"
     CDS             <1..1455
                     /locus_tag="VF547_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX8263591.1"
                     /translation="GREPLCRRPAALPPLPPERADDQQQQNRHGGPPVMAPVMELPPP
                     PPAPPPPPPPMAAEPMAPEASADSSGGESAISVTGSRVAPPTPAPSAPIPPAVDGRSA
                     EARVGLGFHRPRPQPQAGLLTAGDHDDLLNPELDASYAARFFQHSNLRDLPRLDTRRV
                     LTVAVQDGAGRPVPFARVTIHCGDGNSLSLATVADGTAVFYPGLDRLGDRVRVTVPGG
                     AARMVDLAGAGAQRQSFTVRSGAVPIRKFDLMLAIDTTGSMGDEISYLKSELRAILAD
                     IRRAHPGLDIRLGLVAYRDLGDVYVTRTYPFTADLDSMQAHLRAQYADGGGDYPEAMD
                     LALARAVGQDWRPDAVKSLLLVADAPPHDENIGRTWRAAEAARGRRIQIVPVAASGVA
                     DTAEYVMRAMAAATQSRYIFLTDDSGIGNPHAPPAVDCYLVTRLDRLVRRVLDSQISG
                     RRVEPREGEVIRSVGRYDNGKCLLPPDYPIEPQY"
BASE COUNT          224 a          674 c          577 g          224 t
ORIGIN      
        1 ggccgcgagc cgctctgccg ccgccccgcc gccttgccgc ccttgccccc cgagcgggcg
       61 gacgaccagc agcagcagaa ccgccatggc ggcccgccgg tgatggcgcc ggtgatggag
      121 ttgccgccgc cgccgcccgc gcccccgccg ccgccgccgc cgatggctgc ggagccgatg
      181 gcgcccgaag cgagcgccga ctccagcggc ggggaatcgg cgatctcggt gaccggcagc
      241 cgagtcgcgc cgccgacgcc ggcgccgtcg gctccgattc cgcccgcggt ggacggccgg
      301 agcgccgaag cgcgggtcgg cctgggcttc caccgcccgc ggccgcagcc gcaagccggc
      361 ctgctcaccg ccggcgacca tgacgacctg ctcaatcccg agctcgacgc ctcctacgcg
      421 gcccggttct tccagcacag caacctgcgc gacctgcccc ggctcgacac tcggcgggtg
      481 ctcaccgtcg cggtccagga cggggccggc cggccggtgc cgttcgcgcg ggtgacgatc
      541 cactgcggcg acggcaacag cctgtcgctc gccaccgtcg ccgacggcac cgcggtcttc
      601 tatcccggcc tcgaccgcct cggcgaccgg gtccgggtca ccgtcccggg cggcgccgcg
      661 cggatggtcg acctcgccgg ggccggcgcg cagcggcaga gcttcaccgt ccggtccggc
      721 gcggttccga tccgcaagtt cgatctgatg ctggcgatcg acaccaccgg ctcgatgggc
      781 gacgagatca gctatctgaa gtccgagctg cgcgcgatcc tcgccgacat ccggcgggcg
      841 catccgggcc tcgacatccg cctcggcctg gtcgcctatc gcgacctcgg cgacgtctac
      901 gtcacccgca cctatccgtt caccgccgat ctcgactcga tgcaggccca tctgcgcgcc
      961 caatatgccg acggcggcgg cgactatccg gaggcgatgg acctggcgct cgcccgcgcc
     1021 gtcggccagg actggcggcc cgacgcggtc aagtcgctgc tgctggtcgc cgacgctccg
     1081 ccccacgacg agaatatcgg caggacctgg cgcgccgccg aggccgcccg cggccgccgc
     1141 atccagatcg tcccggtcgc cgcctcgggc gtcgccgaca ccgccgaata tgtcatgcgc
     1201 gcgatggccg cggcgaccca gtcgcgctac atcttcctca ccgacgacag cggcatcggc
     1261 aacccgcacg ccccgcccgc ggtcgactgc tacctcgtca cccgcctcga ccggctcgtc
     1321 cgccgggtcc tcgacagcca gatctccggc cgccgggtcg agccgaggga aggcgaggtg
     1381 atccgcagcg tcggccgcta cgacaacggc aaatgcctgc tcccgccgga ctatccgatc
     1441 gagccgcaat attaggggcg gcgaccgggc cgcgacgaat gctccattgt ggaggcatag
     1501 gagcgcgcgc gccgaacgct gaaggtctat aggctgcccc gtgcagcgag ggcgtttcat
     1561 gcccctggtc gaacccactg tcggtgctcg gatccgggcc gcctcggcgc gccgccggat
     1621 gcgtcgcttc gtcggccggt tcacggttgc cgacataggc gaggcctggc gccggatatc
     1681 ggctctcgga gccgagcgg
//
LOCUS       DASVQZ010000428         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_764815, whole genome shotgun sequence.
ACCESSION   DASVQZ010000428 DASVQZ010000000
VERSION     DASVQZ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764815"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VF547_12025"
     CDS             <1..998
                     /locus_tag="VF547_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8263592.1"
                     /translation="EAFTALLNARKTLLAGFTTVRDLGNEPRMITTLRDVIEAGQFAG
                     PTIVAAGRSISVSGGHGDVNGLNRDLTEIGLARADNTCNGADDCRRAVRRQISAGADV
                     IKFAATGGVLSNVAGGLNQQMMADEMRAIVETARTFGRKVAAHAHGVDGINAALEAGV
                     DSIEHGTFTDDRTFALYRRTGAYYVPTLVAPAAAVADGERGALTPAQFDKAKQAAGNA
                     ERSFARAVRERVNIAFGTDSGVAPHGLNAKEFVLMVKNGMAPAAAIRSATVAAATLLG
                     RADRIGTIEAGKDADIVAVRGDPTADIGLLENVQFVMKHGRVHKLGGERQLTSAD"
     gene            complement(1141..2424)
                     /gene="murA"
                     /locus_tag="VF547_12030"
     CDS             complement(1141..2424)
                     /gene="murA"
                     /locus_tag="VF547_12030"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8263593.1"
                     /translation="MDRIIIRGGRRLEGRIPISGAKNAALTLLPCALLTDEPLTLRNL
                     PRLADVDSFGHLLNQLGVSITVEGARADEFGRVMTLRASKLVSTVAPYDIVRKMRASI
                     LVLGPLVARAGEATVSLPGGCAIGMRPVDLHLKALEALGAEIEVAAGYVRAKAPKGGL
                     QGGTITFPFVSVGATENALMAAVLAKGETVIENAAREPEITDLAKCLIAMGADIEGLR
                     TDTLTIRGKSRLHGATYAVMPDRIEAGSYACAAGITGGSLELVGARKDTMPSILSGLS
                     DSGLIVEDTADGVRVTADGALKPLSISTAPYPAFPTDMQAQFMAMLTLAEGTSLLTET
                     IFENRYMHVPELNRMGAEIEVRGRSAVVRGVEGLVGAEVMATDLRASMSLVLAGLAAQ
                     GETQVHRVYHLDRGYERLEEKLQAVGADIERASDG"
BASE COUNT          417 a          909 c          936 g          408 t
ORIGIN      
        1 acgaggcctt cacggcgctg ctcaacgctc ggaagacgct tctggccgga ttcaccacgg
       61 tacgcgacct cggcaacgag ccgcggatga tcacgaccct gcgggacgtc atcgaggccg
      121 gccagttcgc cggtccgacc atcgtcgcgg cgggccgcag catctccgtc tccggcggcc
      181 atggcgacgt caacgggctc aaccgcgacc tgaccgagat cggcctcgcc cgcgccgaca
      241 acacctgcaa cggcgccgac gactgccggc gggcggtgcg ccggcagatc tcggccgggg
      301 ccgacgtcat caagttcgcc gcgacgggag gcgtgctcag caacgtcgcg ggcggcctca
      361 accagcagat gatggccgac gagatgcgcg cgatcgtgga gacggcgagg accttcggcc
      421 gcaaggtcgc cgcccacgcc catggcgtcg acggcatcaa cgcggcgctc gaggcgggcg
      481 tcgattcgat cgagcacggc accttcaccg acgacaggac cttcgccctc taccgccgga
      541 ccggcgccta ttacgtgccg acgctcgtcg ctccggccgc cgcggtcgcg gacggcgagc
      601 ggggcgctct gactccggcc cagttcgaca aggcgaagca ggccgccggc aatgccgagc
      661 gcagcttcgc ccgggcggtg cgcgagcggg tcaacatcgc cttcggaacc gacagcggag
      721 tcgctcccca cggcctcaac gccaaggagt tcgtgctgat ggtgaagaac ggcatggctc
      781 ccgccgcagc gatccgctcc gccacggtcg ccgccgcgac cctgctcggc cgagccgacc
      841 ggatcgggac gatcgaggcc ggcaaggacg ccgacatcgt cgccgtgcgc ggcgatccca
      901 ccgccgacat cgggctgctc gagaacgtgc agttcgtgat gaagcacggc cgggtccaca
      961 agctcggcgg agagcgccag ctgacttcgg cggattgacg cccttcgaag cgtccttcga
     1021 tacgccggtt cgacaggctc accggctact caggatgagc ggtagagaat gatatcgagc
     1081 gctcatcctg agtagagact gagcgaaggc gaaggctcgt atcgaaggac cgtccggatc
     1141 ttagccgtcg ctggcgcgct cgatgtcggc gccgacggcc tggagcttct cctccaggcg
     1201 ctcgtagccg cggtcgagat ggtagacccg gtggacctgg gtctcgccct gggcggcgag
     1261 gcccgcgagg acgaggctca tcgaggccct gaggtcggtc gccatgactt cggcgccgac
     1321 caggccctcc actccgcgca ccacggccga acggccccga acctcgatct ccgcgcccat
     1381 tcggttgagc tcggggacgt gcatgtagcg gttctcgaag atcgtctcgg tgagcaggct
     1441 ggtgccttcg gccagggtca gcatcgccat gaactgggcc tgcatgtcgg tcgggaaggc
     1501 gggatagggg gcggtcgaga tggacagggg cttcagggcg ccgtcggcgg tgacccgaac
     1561 cccgtccgcc gtgtcctcga cgatcaggcc ggaatcgctg aggccggaga ggatcgaggg
     1621 catggtgtcc ttgcgcgctc cgaccagctc cagcgagccg ccggtgatcc cggcggcgca
     1681 ggcgtagctg cccgcctcga tcctgtccgg catcaccgca taggtggcgc cgtggagccg
     1741 gctcttgccg cggatcgtca gggtgtcggt cctcagcccc tcgatgtcgg cgcccatggc
     1801 gatcaggcat ttggcgaggt cggtgatctc gggctcgcgg gcggcgttct cgatgacggt
     1861 ctcgcccttg gcgaggacgg cggccataag cgcgttctcg gtcgcgccta cggagacgaa
     1921 cgggaaggtg atggttccgc cctgaaggcc gcccttcggc gccttcgccc tcacatagcc
     1981 ggcggcgacc tcgatctcgg cgccgagcgc ctccagcgcc ttgaggtgga ggtcgaccgg
     2041 ccgcattccg atcgcgcagc cgcccggcag cgacacggtc gcctctcccg cccgggcgac
     2101 gagcgggccg agcaccagga tcgaggcgcg catcttgcgg acgatgtcgt agggcgcgac
     2161 ggtcgagacc agcttggagg cgcgcagggt catcacccgc ccgaactcgt cggcccgggc
     2221 gccctccacg gtgatggaga ctccgagctg gttcaagagg tggccgaagc tgtcgacgtc
     2281 ggcgaggcgc ggcaggttcc tgagcgtcag cggctcgtcc gtgagcaggg cgcagggcag
     2341 cagggtcagc gccgcgttct tggcgccgga gatcgggatg cggccctcga gacgcctgcc
     2401 gccccggatg atgattcggt ccatatgggt ccttcgatac gggctttcgc cttcgctcgg
     2461 ccctactcag gatgatcctt cgaaacgggc cttcgccaag ctcagtccct actcaggatg
     2521 agcgggaagc tccagtccgg ttggaagccc gcaccgctca tcctgagcag ccggtgggcc
     2581 tgtcggaagc ggcgtatcga aggatcaagc cttttcgagc gtgcactgca aggggtgctg
     2641 gttctgccgt gcgtagtcga tcacctgggc
//
LOCUS       DASVQZ010000429         5500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_87042, whole genome shotgun sequence.
ACCESSION   DASVQZ010000429 DASVQZ010000000
VERSION     DASVQZ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87042"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VF547_12035"
     CDS             <1..548
                     /locus_tag="VF547_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8263594.1"
                     /translation="PAEYFRDLAGLNVRPLGFPDSYAPMVGHADGWPVGYDPEEGRAW
                     LTHCYGYVGVGRDLAPDTGTGGELYAVIGHAPRHLDLNIAVIGRVVEGMALLSARPRG
                     TEALGFYKDPAQAIPIARARLAADMPAAERPAYEAMRSDSLAFSQYVTGRANRGGEFF
                     QRPAGGVDICNAQVPVRKTPA"
     gene            complement(923..1189)
                     /locus_tag="VF547_12040"
     CDS             complement(923..1189)
                     /locus_tag="VF547_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263595.1"
                     /translation="MATSILEARGGCIIPQADQRGYHIVYREHETNYCPGCGRTHWYI
                     GRSMAECAFCTTALPLENAPMIAGGAIVRTIRRPVFGTEHAYAA"
     gene            complement(1414..1569)
                     /locus_tag="VF547_12045"
     CDS             complement(1414..1569)
                     /locus_tag="VF547_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263596.1"
                     /translation="MEGLELETVVLVSGLVLASLALPMTAVAIHQRRRRSYERSAGQR
                     RKEKIRL"
     gene            complement(1796..1984)
                     /locus_tag="VF547_12050"
     CDS             complement(1796..1984)
                     /locus_tag="VF547_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX8263597.1"
                     /translation="MNGPFTLDRANGKVMGVCAGLADATGADATLIRLAAVLSLFLLG
                     PVAVLLYLVAGWVAPERG"
     gene            complement(2374..4320)
                     /locus_tag="VF547_12055"
     CDS             complement(2374..4320)
                     /locus_tag="VF547_12055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8263598.1"
                     /translation="MARTILKLAAAALLVSLASGPARPEAPVPPPPLIPAEAFGALPF
                     FTSPEISPDGTLLVAGSVSEGRKAVVLADLTKADYALARIDLSDRFEMLWARWAGNRR
                     VLLSLIIPTKLYGTEIRTSRLFLFDVATRTVRPLDGKIGGIDGDDVIHVDPQGKYILL
                     SAQRSIFETPAVLHVDLDTLKARQLVKPYPGVWSWYADRDGIVRAGLGTQGGRWWLFY
                     RESAQAGFRKVGTGRRPDNQSLTDVEKLLPVAGSDKGYAIANKATGRYGVYRYDFVTN
                     ALGEPVFEHREVDVDSVGFSPRSGDPDSVFYADDRDRVAWLDPAMKAVQARIDRLLPG
                     AVNRIVSRDAVDRRMVVWSGGASDPGTYYVFDRTKGELRELARPYAALDGKPLAPVRP
                     VRYAARDGLAIPAYLTLPLGRPAEGLPLIVMPHGGPFIRDKWAYEPWAQFLANRGYAV
                     LQPNFRGSTGYGKAFVEAASGQFGRKMQDDLDDGVRWLVERGTVDPKRVCILGASYGG
                     YAAMWAAVRNPDLYRCAISFAGISDVGEMLRFDRSQWIAQRYYRDWRDRIRGEGGFDL
                     AKVSPIARAAEIRIPLLIAHGQKDANVPFAQSKKLHEALLRAQVPHDFVVYPEEGHGF
                     SSVDDSVDFLKRVERFLARHNPAG"
     gene            complement(4475..>5500)
                     /locus_tag="VF547_12060"
     CDS             complement(4475..>5500)
                     /locus_tag="VF547_12060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8263599.1"
                     /translation="YADDRDRIFWIDPEMRSVQARIDKALPNAVNRIVSRDAADTKMI
                     VWSTGAADPGTYYLYDRSSRAMSELARPYGALEGKTLAAVETVRYPARDGLVIPAYLT
                     KPPGRPEAALPLIVMPHGGPFARDKWAYDGCAQFLANRGYVVLQPNFRGSTGFGKAFV
                     DAATGQYGRKMQDDLDDGVRWLAGRGLVDPKRVCIMGASYGGYAAMWGAARNPDIYRC
                     AISFAGISEVRTMLRYDPSTGIARRYYKDWRDRIRGEEGFDLAAVSPLNRAAAIRVPL
                     LIAHGEEDHVVPVAQSRNMHEAMRRAKIDHEFVVYPGEGHGFKKAANSIDFLKRVEAF
                     LALHNPS"
BASE COUNT          902 a         1793 c         1959 g          846 t
ORIGIN      
        1 cgcccgccga atatttccgg gatctggccg gcctgaacgt ccggccgctc ggctttcccg
       61 acagctatgc gccgatggtc ggccacgccg acggctggcc ggtcggctac gatccggagg
      121 aggggcgggc ctggctcacc cattgctacg gctatgtcgg ggtcggccgc gacctcgcgc
      181 ccgacaccgg caccggcggc gagctctacg cggtgatcgg ccacgcgccg cgccacctcg
      241 acctcaacat cgcggtgatc ggccgggtcg tggaggggat ggcgctgctg tcggcgcggc
      301 cgcgcgggac cgaggcgctg ggcttctaca aggatccggc ccaggcgata ccgatcgccc
      361 gcgcccggct cgccgccgac atgcccgcgg ccgagcggcc ggcctatgag gcgatgcggt
      421 ccgacagcct cgccttcagc caatatgtga cgggacgcgc caatcgcggc ggcgaattct
      481 tccagcgccc ggccggcggg gtcgacatct gcaacgcgca ggttccggtt cgcaagacgc
      541 cggcctagag cgtgatcggg cccggtgcgc gaagtcgaac aggcgtatcg aagggcggtc
      601 cttcgatacg ggccttcgcc ttcgctcagt ccctactcag gatgagcggt tcggcggaag
      661 gcgatcacgc tccagctgga tcaccctcac ccgttcgccc tgagcctgtc gaagggcttt
      721 gcttgtcgtg ccatgagaga aaaggagaca gcttcgacga gctcagcccg aacggagaca
      781 gggtggcgat ccctcgacgc ccttcggatg acgtccgggg ggcgttgtcg cggatgacgg
      841 gaggttacgc gccggcccct cgcgtagggg atgttaaggg gccggcgcga cggcggccgc
      901 cgcgagggcg ccgccagcga tctcaggccg cgtaggcgtg ctcggtgccg aacaccgggc
      961 ggcggatggt gcgaacgatc gcgccgccgg cgatcatcgg cgcgttctcg agcggcaggg
     1021 ccgtcgtgca gaaggcgcat tccgccatcg agcggccgat gtaccagtgg gtacggccgc
     1081 agccggggca gtagttcgtc tcatgctcgc gatagacgat gtgatagccg cgctgatccg
     1141 cctgggggat gatgcagccg ccacgcgcct cgaggatact cgtcgccata cttggctcct
     1201 ttcaacacac ttggcagccc aacgaccgac gagtctgaag agtttcatgg ctttacgatg
     1261 aacggagccg gagcgcgggc cgcccgcccc tcgcggacgg ggcggaaaag cgcggaaatc
     1321 tgcggcgacg agccgttcgg aagcgtcgcc gaaccgagtc gcgccgacct caaaaaattt
     1381 tcgtggggac cgctttccgg ggccgcggcc gggtcagagc cggatctttt ccttgcggcg
     1441 ctggccggcg gagcgctcgt aggaacgccg gcggcgctgg tgtatggcga cggcggtcat
     1501 gggcaaggcg aggctcgcca gcaccagccc gctgaccagg accaccgtct caagctccag
     1561 cccttccatc gatcgctcct cccgagccca attcgtaccg ggaatcggac tcgccgtcac
     1621 cccggtcctt gcggccgcgg gccgaaaaag aggatagcgc gcggcgcggc ggcgccgaag
     1681 cgaaattgga accggatcgg ggcgtgccgg ggctggccgg gcggcctcgc catggcgctc
     1741 gtcgtggagg agcggggcgc tcgattcgcg acttgccgca gtccgcgcag ggcgctcagc
     1801 cgcgctcggg cgcgacccag ccggcgacca gatagagcag gaccgcgacg ggtccgagca
     1861 ggaacaggct gagcacggcg gccaggcgga tgagcgtcgc gtcggctccc gtcgcgtcgg
     1921 cgaggccggc gcagacgccc atcactttgc cattggctct gtcgagggtg aaaggtccgt
     1981 tcatggggcc tttccttcct tgttgttgct tcgggtagcg atttctcgcg ttctcgcccg
     2041 tgggccggac ggtcagacga gctgaccggc cgggccgacg gcggcggtga tgaacaggct
     2101 ggcggcgaag agggcgccga tcgccgagac caggaactgc tggatgttga ggttgaaggt
     2161 cattgtaagt ctccctgatg cggccgtccg gaccatccgc cggccatgcc caacaggttt
     2221 gcagggagcg tgccaaacgc agcaaatggc ggaaatccgc cattctcgcc tccagccaat
     2281 ggcgcggcgg caaatcgccg tctcaaaagt tgggattttt cgccgacaat tggcggccgc
     2341 ttgcggacgg cttgatgtcc gcttccggac agcctagccg gccgggttgt gccgggccag
     2401 gaagcgctcg acccgcttga ggaagtcgac cgaatcgtcg acgctcgaga aaccgtggcc
     2461 ttcctcggga tagacgacga agtcgtgcgg cacctgcgcg cgaagcagcg cctcgtgaag
     2521 cttcttcgac tgcgcgaagg gcacgttggc gtccttctgg ccgtgggcga tcagcagcgg
     2581 gattcggatt tcggcggcgc gggcgatcgg cgacaccttg gcgagatcga atccgccctc
     2641 gccgcggatg cggtcgcgcc agtcgcggta atagcgctgc gcgatccact gcgaacggtc
     2701 gaagcgaagc atctcgccga cgtcggagat cccggcgaag ctgatcgcgc agcgatagag
     2761 gtcgggattg cgaaccgccg cccacatcgc cgcatagccg ccgtaggaag cgccgaggat
     2821 gcatacccgc ttcggatcga ccgtgccgcg ctccacgagc cagcgcacgc cgtcgtcgag
     2881 atcgtcctgc atcttccggc cgaactggcc cgacgcggcc tcgacgaagg ccttgccgta
     2941 gccggtcgag ccccggaaat tgggctgcag caccgcatag ccgcgattgg cgaggaactg
     3001 cgcccaaggc tcataggccc atttgtcgcg gatgaacggc ccgccgtgcg gcatcacgat
     3061 cagcggcagg ccctcggcgg ggcgcccgag cgggagggtc agataggcgg ggatggcgag
     3121 gccgtcccgg gcggcgtagc gcaccggccg aaccggcgcg agcggcttgc cgtcgagcgc
     3181 cgcatagggc cgcgccagct cccgaagctc gcccttggtc cggtcgaaca cataataggt
     3241 gcccggatcc gaggctccgc ccgaccagac caccatccgg cggtcgacgg cgtcgcgcga
     3301 gacgatccgg ttgaccgcgc ccggcagcag gcggtcgatc cgggcctgga ccgccttcat
     3361 cgccgggtcg agccaggcga cccggtcgcg gtcgtcggcg tagaagaccg aatcgggatc
     3421 gccggaacgc ggcgagaagc cgacgctgtc gacgtcgacc tcccgatgct cgaacaccgg
     3481 ctcgccgagc gcgttggtga cgaaatcgta gcgatagacg ccgtagcggc cggtcgcctt
     3541 gttggcgatc gcatagccct tgtcgctgcc cgccaccgga agcagcttct cgacgtcggt
     3601 caggctctga ttgtcagggc gccggccggt gccgaccttg cggaagcccg cctgcgcgct
     3661 ctcgcggtag aagagccacc agcggccgcc ctgggtgccg aggccggcgc ggacgatgcc
     3721 gtcgcggtcg gcataccagc tccacacgcc gggatagggc ttgaccagct gcctggcctt
     3781 gagcgtgtcg agatcgacat ggagcacggc gggcgtctcg aagatcgagc gctgggcgga
     3841 cagcaggata tatttgccct gcgggtcgac gtggatgacg tcgtcgccgt cgattccgcc
     3901 gatcttgccg tcgagcgggc gcaccgtgcg ggtggcgacg tcgaacagga acagccggct
     3961 ggtcctgatc tcggtgccgt agagcttggt cgggatgatc aggctcagaa gcacgcgccg
     4021 gttgccggcc cagcgcgccc acagcatctc gaagcggtcg gacagatcga tccgggcgag
     4081 ggcgtaatcg gccttggtga gatcggcgag gacgaccgcc ttgcggcctt ccgacaccga
     4141 gcccgcgacg aggagggtgc cgtcgggcga gatctccggg gaggtgaaga agggaagggc
     4201 gccgaacgcc tcggccggaa tgagcggcgg gggcgggacg ggcgcctcgg gccgcgccgg
     4261 acccgaagcg agcgaaacca gcagcgccgc ggccgccagc ttcagaatcg ttcgcgccat
     4321 gctcaatccc ccgaggatgc gagcagctca ggtcgcagga ttatacctct ccgggacgtt
     4381 tttcacctgc tttagcagcc ccactgaaaa gcctttgaaa cgagacgtat agctgagatc
     4441 gggagggcag cgcaattgac cgccgcaggg ccgctcagga ggggttgtgg agcgccagga
     4501 aagcctcgac gcgcttgagg aagtcgatcg aattggccgc tttcttgaag ccgtggcctt
     4561 cgcccgggta gacgacgaat tcgtggtcga tcttcgctcg ccgcatcgcc tcgtgcatat
     4621 tgcgcgactg ggcgacgggg acgacgtgat cctcctcgcc atgggcgatc agcagcggca
     4681 cccgtatcgc cgcggctcgg ttcagcggcg agacggcggc gagatcgaag ccctcctcgc
     4741 cgcggatgcg gtcccgccag tccttgtaat aacggcgggc gatcccggtc gagggatcgt
     4801 agcggagcat ggtccggacc tcggagatgc cggcgaagct gatcgcgcag cggtagatgt
     4861 cggggttcct tgcggcgccc cacatcgccg catagcctcc gtaggaggcg cccatgatgc
     4921 agacccgctt cggatcgacc aggccgcggc ccgccagcca gcggactcca tcgtccagat
     4981 cgtcctgcat cttgcggcca tattggccgg tggccgcgtc gacgaaggcc tttccgaagc
     5041 cggtcgagcc gcgaaaattg ggctgcagga cgacatagcc gcgattggcg aggaactgag
     5101 cacagccgtc ataggcccat ttgtcgcggg cgaacgggcc tccgtggggc atgacgatca
     5161 gaggaagcgc ggcctcgggc cgtcccggcg gcttggtcag ataggccggg atgacgaggc
     5221 cgtcgcgcgc cggatagcgg accgtctcga ccgccgcgag cgtcttcccc tcgagcgcgc
     5281 cgtagggcct ggccagctcg ctcatcgccc ggctggaccg gtcgtagaga taatatgtgc
     5341 cgggatcggc ggcgccggtc gaccagacga tcatcttggt gtcggcggcg tcgcgcgaga
     5401 cgatccggtt gacggcattc ggaagggcct tgtcgatccg ggcctgaacg gagcgcatct
     5461 caggatcgat ccagaagatg cggtcccggt cgtcggcgta
//
LOCUS       DASVQZ010000430        13080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_475603, whole genome shotgun sequence.
ACCESSION   DASVQZ010000430 DASVQZ010000000
VERSION     DASVQZ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475603"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VF547_12065"
     CDS             complement(<1..1085)
                     /locus_tag="VF547_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8263600.1"
                     /translation="MGSARKSARLGLIAAMLLAGAGCSTTDGATAGETGRWPAVRSRV
                     RSDPAAEARIDAVLRGMSLEEKVAQIIQPDISSVTPDDVRRYKFGSILAGGNSSPGGR
                     ETAPPGEWLKLADRFWEASRSADWAGSNIPLMWGIDAVHGHANIVGATIFPQNIGLGA
                     TRNPELIRRIGEATAREMVTTGIDWDFSPTLAVVRDDRWGRTYEGFSEDSEIVRAYAG
                     RMVEGLQGRAGTREFMGPGRVIATAKHFVGDGGTAGGKDQGDNPSSPEQLRDIHGAGY
                     PPAIEAGVQAVMASFSSVRGVKVHGDRDLLTGALKADMGFDGLLVGDWNAHGQVPGCT
                     NVSCPASINAGLDMFMAPDSWKGLYDNT"
     gene            1268..1402
                     /locus_tag="VF547_12070"
     CDS             1268..1402
                     /locus_tag="VF547_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263601.1"
                     /translation="MSTALSRRVPDETVASLLARIREGRLAGHPSYVLRMQFRTSAQA
                     "
     gene            1419..2273
                     /locus_tag="VF547_12075"
     CDS             1419..2273
                     /locus_tag="VF547_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HEX8263602.1"
                     /translation="MGALLIGPVIDGAFFDLFDGLKASTVEHASRRWHRIIPAYSPWL
                     CFAPLLDEVPAELIYLQPPVRGGLIADPLILSTMADFLAGPGRPAIDELPGETAAWLP
                     GPSGAGHAFDGALAIIARTAGTVSDLYSRTVDYVVPLGGGRNRGFSTHLARGAIFRSL
                     PAESDAWDVAIDLIHEVGHQVLMTWQSVDPILSSPHDAPVFSEIRRADRPAIQSFHAA
                     VALAFMRFLELRHPDEPGMRAAAARRGASYSGSLSLSLELALKVVREACAVTDLGRRM
                     IGEMEAVV"
     gene            2344..3114
                     /locus_tag="VF547_12080"
     CDS             2344..3114
                     /locus_tag="VF547_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8263603.1"
                     /translation="MPGLAALFFVTALIYGAVGFGGGSTYNALLVLAGTDFRAVPVIA
                     LGCNIVVVTMGSWRFARSGHVDLRRIWPLFVLSIPCAWLGGRLVVAETLFVGLLALSL
                     FASGLLMLWQPLWRKEAAARRRSRWLEPAAGGAIGFLAGLVGIGGGIFLTPLLYMLRW
                     GPPKAIAGTSAVFILANSLSGLAGQLAKGSGAAAADALARHWPLFPAVLAGGLIGATV
                     GSGRLDPKFVRILTALLILYVAARLAVRFYGEAVAGGG"
     gene            complement(3270..4538)
                     /gene="guaD"
                     /locus_tag="VF547_12085"
     CDS             complement(3270..4538)
                     /gene="guaD"
                     /locus_tag="VF547_12085"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEX8263604.1"
                     /translation="MKAFRGEILSVPDDPAAAGADAIRHFEDGLLVVEDGLVLACGAH
                     AELADRFADVPAERLAGLVVPGFVDAHVHYPQIECIASHGEQLMQWLERHIFPAEKAF
                     ADRGHADSVAAFFLDELLRHGTTSALVFATVHAGSVDALFEAALERDMRIVSGKVLMD
                     LGPEGLRDTVESGRADTEALIARWRGRGRLGYAVTPRFVPTSSVEQLADAGAIVAAHP
                     DVLMHTHLSENRGEIEFVASRFPEAADYLDVYDRFGLVGPRSVFAHCVHMSDRALARM
                     AEAGSGIAFCPTSNMFLGSGLFDLAQADRHGARIGIGTDIGAGTSLSVLHTLGEAYRT
                     CQLRGASLDPFRALHLATAGGARVLGIADRVGALLPGQEADFVVLDERATPLLQRRCA
                     GASLHDRLFALQVLGDDRAVAATYLRGRRA"
     gene            complement(4535..5467)
                     /gene="xdhC"
                     /locus_tag="VF547_12090"
     CDS             complement(4535..5467)
                     /gene="xdhC"
                     /locus_tag="VF547_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEX8263605.1"
                     /translation="MADWTREARDALSRGPAALVTVLAAEGSAPRGPGARMVVTPTGL
                     AGSIGGGRLEHQAMAQARAILGLEPGSWRVQDYPLGPLLGQCCGGRVRLLVERLDSVP
                     EGEGPFEVTLAERVERRSLTGDGRGPAADSGPGAGSLRRGAIAARGPLPTAGAHFIEP
                     VESDSLLVLLFGAGHVGRAIAARADGLPLRIAWHDSRAEMAETPGVVLADEAAMAAAA
                     AQAPEGSAVVIMTHDHGLDYRLVAAALGGRAAFVGLIGSKTKRARFLKRLAADGVDAG
                     RLTCPIGLPGISGREPEVIAIATLAQLLMLRSPS"
     gene            complement(5675..7924)
                     /gene="xdhB"
                     /locus_tag="VF547_12095"
     CDS             complement(5675..7924)
                     /gene="xdhB"
                     /locus_tag="VF547_12095"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEX8263606.1"
                     /translation="MNRPLPHDSADLHVAGAAAYVDDIPEAPGTLHLAFGLAADGHSR
                     LAGLELSEVRAAPGVVAVFTAADIPGENNVGPVFHDDKLFAEDEILYPGQPLFVVAAT
                     SARAARIAARLARVEAEPLAALVTIGDAFDAGSDLEPAQRMARGDADAALERARHRLS
                     GTLEMGGQEHFYLEGQAALATPGERGQIHVVSSTQHPSEVQHLIAELLGLSSADVTVE
                     VRRMGGGFGGKETQAAAYAAACALVAAKTGRPAKIRADRDDDMVMTGKRHDFTAHYDV
                     GFDGEGRIEGIRITLAARCGATVDLSLAISDRAMFHADNCYWLPAVEIVSHRLKTHTV
                     SNTAFRGFGGPQGMMAIERVMDAIAAHLGLDPLAVRQANLYGPGRNVTPYHMTVEDNV
                     APELIAELAERAGYAERRAEVEAFNASHQVLKKGLALTPVKFGISFTTTHLNQAGALV
                     LVYADGSIHLNHGGTEMGQGLMIKVAQVVADVFAVPVDKVKVSSTRTDKVPNTSATAA
                     SSGSDLNGMAAKNAAEVIKERLDTFAAGQEGSEEWSFAELCRRAHLARIPLLATGFYA
                     TPKIHYDRATHSGRPFLYFAYGAALSEVMIDTLTGEHRVVAVDILHDVGRSLNPAIDL
                     GQIEGGFIQGMGWLTTEELVFDARGRLLTHAPSTYKIPTANDRPARMEIAIWERGRNE
                     EPTIHRSKAVGEPPLMLAISVFSALTQAVAAAAPGKGLPKLDAPATPERILGAIDELR
                     ARTHDRRPS"
     gene            complement(7959..9326)
                     /gene="xdhA"
                     /locus_tag="VF547_12100"
     CDS             complement(7959..9326)
                     /gene="xdhA"
                     /locus_tag="VF547_12100"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEX8263607.1"
                     /translation="MIRFLLDDRVIEIEDGDPTATVLDHLRYGLRRTGTKEGCAEGDC
                     GACTVLLGELDGDRVQWRAVNSCILFLPMLDGKALRTVESLGGEHPVQRALVERHGSQ
                     CGFCTPGFVMSLYGRSLGALGTRGVPVGDVIAGNLCRCTGYGPILAAGEAVPPGAGND
                     EATVAALRALRPAYGHPASADELAELLLANPETRIIAGATDVGLWVTKQLRDLGPTVF
                     IGDIADLRRIEERPDDVTLGAAVRYSEAREVLAGLHPDLGELIRRIGGVQVRNAGTIG
                     GNIANGSPIGDMAPALIAIGAELVLRRGSERRTVALENFFLSYGQQDLAPGEFVESVR
                     IFRPPASALIRIVKLSKRFDSDISAVCGAFVLRIADGIVREARVAFGGMAGIPARAPG
                     CEAALIGRAWTEATVEAAAQALADDYTPLDDLRGSAAYRRKVAANLLRRIWAEQSEPA
                     SVLHG"
     gene            complement(9323..10483)
                     /locus_tag="VF547_12105"
     CDS             complement(9323..10483)
                     /locus_tag="VF547_12105"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX8263608.1"
                     /translation="MGVDLGEWLNLAIRWLHLTAGIAWIGSSFYFVWLDNHLVKPAEG
                     DASGELWSVHGGGFYHNRKYQVAPSHMPERLHWFKWEAYFTWISGFSLLVLIYYVGAQ
                     SFLIDPAKVMMSPGAAIAVGLAALALGWLVYDGLCRSPLGSNDLLLGAFWFVCLVWAA
                     FLLDHLFSSRAAYLHVGAIVGTAMVGNVFFVIIPNQRRVVADLIAGRTPDPALGAAAK
                     QRSLHNNYMTLPVLFIMISPHYPMTYGAQRPWLVLALLGLTGIAVRHVFNRRGRGRTT
                     GPTSAVAAALALATVSYVTLERRAAAPGRAGTPAFAQIEPILATHCAGCHGADPPPAG
                     VALDSWERVRAASARIKAVAVDSHVMPLGNPTGMTQAERQTLGAWIAAGSPR"
     gene            complement(10486..10821)
                     /gene="uraH"
                     /locus_tag="VF547_12110"
     CDS             complement(10486..10821)
                     /gene="uraH"
                     /locus_tag="VF547_12110"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX8263609.1"
                     /translation="MSSLSTHVLDTMSGRPAAGMAVVLSGPDGVMARGETNADGRCPD
                     LAPAGLAPGRYALRFGVADYFRALGVVLPDPPFLGEVTVDFGIAADGGHYHVPLLVSP
                     YGYSTYRGS"
     gene            complement(10818..11258)
                     /gene="uraD"
                     /locus_tag="VF547_12115"
     CDS             complement(10818..11258)
                     /gene="uraD"
                     /locus_tag="VF547_12115"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX8263610.1"
                     /translation="MSALFEHSPWVEARADARPSSGDRHADLMAVVRDASAEEQLALI
                     RAHPELAGKAAVDGRLTEASAAEQASAGLDRLTPAEFERFHALNAAYREKFGFPFIIC
                     VRLTDKAGILAAMERRLANDRDSEIATAIGQIGEIVRLRLEDRA"
     gene            complement(11255..12262)
                     /gene="alc"
                     /locus_tag="VF547_12120"
     CDS             complement(11255..12262)
                     /gene="alc"
                     /locus_tag="VF547_12120"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEX8263611.1"
                     /translation="MSDDMIMLEELRRHHIDLAQPRLGARVVYATDDFFADKARLIDP
                     AEPVFIPGTSDSNGKWMDGWESRRKRIPGHDWCVVRLGVPGRIAGFEIDTRHFTGNYP
                     PAAQIEVCRSDSEIPDERLGWTAVTERLALKGDDRIYVPVEHDAPATHVRLNIFPDGG
                     VARLRVWGSVAKDWSRVGPDERLDLLAMENGGRGIIANDEHYGRVENLTAPGRGVNMG
                     DGWETRRRREPGHDWAVLKLGAPGRIEQAIVDTAHFKGNYPDRCFLQAAAEAPGSPKE
                     IAAASERWPLLMPPARLEADKVHVFRDSLAELGPVRFVRLNIVPDGGVSRLRLIGRVA
                     R"
     gene            complement(12375..>13080)
                     /locus_tag="VF547_12125"
     CDS             complement(12375..>13080)
                     /locus_tag="VF547_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8263612.1"
                     /translation="IYEYGSRAGVWRLLRLFERYGVPVTVFGVALAMQRHPAVVEAFL
                     EAGHEIASHGWRWINYQRVPEAQECADMERAIEVHRQLTGERPLGWYTGRTSPNTRRI
                     VAEEGGFLYDADDYSDDLPFWARVEGRSHLIVPYSLETNDMRFASANMHTGDQFFAYL
                     RDAFDILYAEGEERPKMMSVGLHCRLAGRPGKLAGLERFLRHALGHADVWLCRRIDIA
                     RHWRETHPHQVEDDFR"
BASE COUNT         1862 a         4596 c         4696 g         1926 t
ORIGIN      
        1 gtgttgtcgt aaagcccctt ccagctgtcg ggcgccatga acatgtcgag gcccgcgttg
       61 atcgaggcgg ggcaggagac gttggtgcaa cccggcacct ggccgtgcgc gttccagtcg
      121 ccgacgagga ggccgtcgaa gcccatgtcg gccttgagcg cgccggtgag caggtcgcgg
      181 tcgccatgca ccttcacccc gcgcacggag gagaagctcg ccatcaccgc ctgcaccccc
      241 gcctcgatcg cgggcggata gccggcgccg tggatgtcgc gcagctgctc gggggaggag
      301 ggattgtcgc cctggtcctt gccgcccgcg gtgccgccgt ccccgacgaa atgcttggcg
      361 gtggcgatca cccggcccgg ccccatgaac tcgcgagtcc cagccctccc ctgcaggccc
      421 tcgaccatcc ggcccgcata ggcgcggacg atctcggaat cctccgagaa gccttcgtag
      481 gtccggcccc agcggtcgtc gcggaccacg gcgagggtcg gcgagaagtc ccagtcgatc
      541 ccggtcgtca ccatctcgcg ggcggtggct tcgccgatgc gccggatgag ctccggattg
      601 cgggtggcgc cgaggccgat attctgcggg aagatggtgg cgccgacgat attggcgtgg
      661 ccgtggaccg cgtcgatgcc ccacatcaac gggatgttgc tgcccgccca gtcggccgag
      721 cgcgacgctt cccagaagcg gtcggcgagc ttcagccatt cgcccggcgg ggcggtctcc
      781 ctgccgccgg gcgagctgtt gcccccggcg aggatcgagc cgaacttgta gcgccggacg
      841 tcgtccggag tgacgctgga gatgtcgggc tggatgatct gcgcgacctt ctcctcgagg
      901 ctcatcccgc gcaggacggc gtcgatccgc gcctcggccg ccgggtcgct ccggactcgg
      961 ctcctgaccg cgggccagcg gcccgtctcg ccggcggtcg cgccgtccgt cgtgctgcag
     1021 ccggcgccgg cgagcagcat cgccgcgatc aggcccagcc ttgcggattt ccgtgcagag
     1081 cccatcgatc cactcccaag ggccttccaa aggcctgacg gcagctagat gataacgcta
     1141 ccagcaggca agcgccgcga tgcccggcgg tgggcggcgg ccgccccccg cgcacctccc
     1201 gagccgaaaa aatccgtttc ctgcgaagcg ctttgaggct atgctggcga ccttgaggag
     1261 aatggtgatg tcgaccgccc tttcgcgccg cgttcccgac gagaccgtcg cgtcgctgct
     1321 tgcccggatc cgcgagggcc gcctcgccgg ccatccgtcc tatgtgctga ggatgcagtt
     1381 ccggacctcg gctcaggcct gaaccgggag gggcccccgt gggggcattg ctgatcggcc
     1441 ccgtcatcga cggagccttc ttcgatctgt tcgacggcct caaggcgtcc accgtcgagc
     1501 acgccagccg aaggtggcat cggatcatcc cggcctacag cccctggctg tgcttcgctc
     1561 ccctgctcga cgaggtcccg gcggagctga tctatctcca gccgcccgtc cgcggcggcc
     1621 tgatcgccga tccgctcatc ctctcgacga tggcggattt cctcgccggc ccgggccggc
     1681 cggcgatcga cgagctgccc ggcgagaccg ccgcctggct gcccgggcct tccggcgccg
     1741 ggcacgcgtt cgacggggcg ctggcgataa tcgccaggac cgcggggacg gtgagcgacc
     1801 tctattcgag aaccgtcgac tatgtcgttc ctctgggcgg aggacggaac cggggcttca
     1861 gcacccatct cgcgcgcggc gcgatctttc gcagccttcc ggccgagagc gacgcctggg
     1921 acgtcgcgat cgacctgatc cacgaggtgg ggcaccaggt gctgatgacc tggcaaagcg
     1981 tcgacccgat cctgtcctcg ccgcacgatg cccccgtctt ctccgagatc cggcgggcgg
     2041 accgccccgc catccagagc ttccacgccg ccgtggccct ggccttcatg cgctttctcg
     2101 agctccggca tccggacgag cccggcatgc gggcggccgc ggcgcggcgc ggcgcctcgt
     2161 acagcggctc gctgtcgctg agcctggagc tggcgctcaa ggtcgtccgg gaggcctgcg
     2221 ccgtcaccga tctcggccgc cggatgatcg gcgaaatgga agcggtcgtc tagcgccccg
     2281 ccggagccgc cctcgcccct cggctatttc ccggcggcgc gaatcgggat aggggcggcg
     2341 cccatgccgg gcctggccgc gctcttcttc gtcaccgctc tgatctacgg agcggtcggc
     2401 ttcggcggcg gatccaccta caatgcgctg ctcgtcctcg ccggaaccga ctttcgcgcg
     2461 gtgccggtga tcgcgctcgg ctgcaacatc gtcgtcgtga cgatggggag ctggcggttc
     2521 gcgcgttcgg gtcatgtcga cttgcggcgg atctggcccc tgttcgtcct ctcgataccc
     2581 tgcgcctggc tcggcggccg gctggtcgtc gcggagacgc tgttcgtggg cctgctcgct
     2641 ttgtccctgt tcgcttcggg cctgctgatg ctttggcagc cgctgtggcg gaaggaggcc
     2701 gcggcgcggc ggcgctcgcg ctggctcgag ccggcggcgg gcggagcgat cggcttcctc
     2761 gccggcctgg tcggaatcgg cggcggcatc ttcctcacgc cgctgctcta catgctgcgc
     2821 tgggggccgc ccaaggccat cgccgggacc agcgccgtct tcatcctcgc caattcgctg
     2881 tcgggcctcg ccggacagct cgccaagggc agcggcgccg ccgccgccga cgccctcgct
     2941 cgtcactggc cgctgttccc cgcggtgctc gccggcgggc tgatcggggc gacggtgggc
     3001 agcggccggc tcgaccccaa attcgtccgg atcctcaccg ccttgctgat cctctacgtc
     3061 gcggcgaggc tcgcggtccg tttctacggc gaggccgtgg cgggcggcgg atgaccggcc
     3121 cgaggggatt gagggcttag gtcgaatcac tcccacccgt tcgccctgag cctgtcgaag
     3181 ggctttcctt gttgttctcc aagagaaagg gagacggctt cgacaagctc agcccgaacg
     3241 gagacagcgc ggactgacgg ccgagcgctc taggcccgac gcccccgcaa ataggtggcg
     3301 gcgacggcgc ggtcgtcgcc caggacctgc agggcgaaca gccggtcgtg aagcgaggcg
     3361 ccggcgcagc ggcgctggag caggggcgtg gcgcgctcgt cgaggacgac gaaatcggcc
     3421 tcctggccgg gcaggagggc gccgacccgg tcggcgatgc ccagcacgcg cgcgccgccc
     3481 gcggtggcga gatgaagcgc ccggaaggga tcgaggctgg cgcctctcag ctggcaggtc
     3541 cggtaggcct cgccgagcgt gtggagcacg gagaggctcg tcccggcgcc gatgtcggtg
     3601 ccgatgccga tccgggcgcc gtggcggtcg gcctgggcaa ggtcgaacag gcccgagccg
     3661 aggaacatgt tgctggtcgg gcagaaggcg atcccagagc ccgcctcggc catccgagcc
     3721 agcgccctgt cgctcatatg gacgcagtgg gcgaagacgg accgcggccc gaccagcccg
     3781 aaccggtcgt agacgtcgag atagtcggcg gcctcgggga agcggctcgc gacgaactcg
     3841 atctcgcccc gattctccga gagatgggtg tgcatcagga cgtccgggtg cgcggcgacg
     3901 atcgccccgg cgtcggcgag ctgctccacg ctcgaggtcg gcacgaagcg cggcgtgacc
     3961 gcatagccga ggcgtccgcg gccgcgccac cgcgcgatca gggcctcggt gtcggcgcgg
     4021 cccgattcga cggtgtcgcg caggccctcg ggccccaggt ccatcagcac cttgcccgag
     4081 acgatgcgca tgtcccgctc gagcgcggcc tcgaacaggg cgtcgaccga gccggcgtgg
     4141 acggtggcga agacgagcgc gctggtcgtg ccgtggcgga gcagctcgtc gaggaagaag
     4201 gcggccaccg aatcggcgtg gccgcggtcg gcgaaggcct tttccgcggg gaagatgtgg
     4261 cgttccagcc attgcatgag ctgctcgccg tgcgaggcga tgcactcgat ctgcggataa
     4321 tggacgtggg cgtcgacgaa gccggggacg acgaggccgg caagccgctc cgcggggacg
     4381 tccgcgaacc tgtcggcgag ctcggcgtga gcgccgcagg cgaggacgag gccgtcctcg
     4441 acgaccagca gcccgtcctc gaaatggcgg atcgcgtcgg cgccggcggc ggcgggatcg
     4501 tcgggaacgc tcagtatctc gccgcgaaag gccttcatga ggggctcctc agcatgagca
     4561 attgggcgag cgtggcgatc gcgatcacct cgggctcccg gccgctgatt ccgggcagcc
     4621 cgatcgggca ggtcaggcgg ccggcgtcga ccccgtccgc cgccagccgc ttcaggaagc
     4681 gcgcgcgctt ggtcttcgat ccgatcagcc cgacgaaggc cgcccggccg ccgagcgccg
     4741 ccgccacgag gcgatagtcg aggccgtggt cgtgggtcat gatcacgacg gccgagcctt
     4801 ccggggcctg ggccgcggcg gcggccatcg ccgcctcgtc ggcgaggacg actccggggg
     4861 tctcggccat ctcggcgcgg ctgtcatgcc aggcgattcg gagcggaagg ccgtcggcgc
     4921 gggcggcgat cgcgcggccg acatggccgg cgccgaagag caggaccagc agcgaatcgc
     4981 tttcgaccgg ctcgatgaaa tgcgctcccg cggtcggcag cggacctctc gccgcgatcg
     5041 ctccccggcg aagggagccg gcgcccgggc ctgagtccgc ggccgggccc cgtccgtcgc
     5101 cggtgagcga gcggcgctcg acccgctcgg ccagggtgac ctcgaacggc ccctcgccct
     5161 ccggcacgct gtcgagtcgc tcgaccagca gccggaccct tccgccgcag cattggccga
     5221 ggagcgggcc gagcggatag tcctggaccc gccagcttcc cggctcgagg ccgaggatcg
     5281 cccgcgcctg cgccatcgcc tgatgctcga gcctgccgcc gccgatcgac ccggcgaggc
     5341 cggttggcgt caccaccatc cgcgcgcccg gcccgcgcgg ggcggagcct tcggcggcga
     5401 ggacggtgac gagcgcggcg gggccgcggc tcagggcgtc ccgggcctcg cgggtccagt
     5461 cagccattgc gagcgctcaa atcctccccg agatttctcg gggaggggga ccatgcgaag
     5521 catggtggag gggcctctct gaccctgaag aacccctcca ccgcgcttcg ccgtaggcgc
     5581 agtttatcct gagcgacgcc gcaggcggcg tcgaagggcg cggtccccct cccccggaaa
     5641 tccgggggag gaattgagag aactcgcgag cccctcacga cggcctgcgg tcgtgagtcc
     5701 gcgccctgag ctcgtcgatc gcgccgagga tccgctccgg cgtggccggg gcgtcgagct
     5761 tcggcaggcc cttgcccggc gcggcggcgg cgaccgcctg ggtcagggcc gagaagaccg
     5821 agatggcgag catcaggggc ggctcgccga ccgccttgga gcggtggatg gtgggctcct
     5881 cgttgcgccc ccgctcccag atcgcgatct ccatgcgcgc cgggcggtcg ttggcggtcg
     5941 ggatcttgta ggtcgacggc gcgtgggtga gcagccggcc gcgcgcgtcg aacaccagct
     6001 cctcggtggt cagccagccc atgccctgga tgaagccgcc ctcgatctgg ccgaggtcga
     6061 tcgccgggtt gagggagcgg ccgacgtcgt ggaggatgtc gaccgcgacg accctgtgct
     6121 cgccggtgag cgtgtcgatc atgacctcgc tcagggcggc tccataggcg aaatagagga
     6181 acggacggcc gctgtgggtc gcgcggtcgt aatggatctt gggggtcgcg tagaagccgg
     6241 tcgcgagcag cgggatgcgc gcgagatgcg ccctccggca gagctcggcg aagctccact
     6301 cctccgatcc ctcctggccg gcggcgaacg tgtcgagccg ctccttgatg acctcggcgg
     6361 cattcttcgc ggccatcccg ttgaggtcgg agcccgagga ggcggcggtg gccgaggtgt
     6421 tggggacctt gtcggtgcgg gtcgagctga ccttcacctt gtcgaccgga actgcgaaga
     6481 cgtccgcgac gacctgggcg accttgatca tcaggccctg gcccatctcg gtgccgccgt
     6541 gattgaggtg gatcgagccg tcggcataga cgaggaccag cgccccggcc tggttgagat
     6601 gggtggtggt gaagctgatc ccgaacttca ccggcgtcag ggcgagaccc ttcttgagga
     6661 cctggtgcga ggcgttgaac gcctccacct ccgcccggcg ctcggcatag ccggcgcgct
     6721 cggcgagctc ggcgatcagc tcgggcgcga cattgtcctc gaccgtcatg tgatagggcg
     6781 tgacgttacg gcccgggccg tagagattgg cctggcgcac cgcgagcgga tccagcccga
     6841 gatgggcggc gatcgcatcc atcacccgct cgatcgccat catcccctgc ggcccgccga
     6901 agcctcggaa ggccgtgttg gagacggtgt gggtcttcag gcggtgcgag acgatctcca
     6961 ccgccggcag ccaatagcaa ttgtcggcat ggaacatggc gcggtcgctg atcgcgaggc
     7021 tgaggtcgac cgtggcgccg cagcgcgcgg ccagcgtgat ccggatgccc tcgatccggc
     7081 cttcgccgtc gaagccgacg tcgtaatggg cagtgaaatc gtggcgcttg ccggtcatga
     7141 ccatgtcgtc gtcgcggtcg gcccggatct tggccggccg gcccgtcttg gcggcgacga
     7201 gagcgcaggc cgcggcatag gcggcggcct gggtctcctt gccgccgaag ccgccgccca
     7261 tccgccgcac ctcgacggtc acgtcggcgc tgctgaggcc gagcagctcg gcgatcagat
     7321 gctggacctc gctcggatgc tgggtcgagc tgacgacgtg gatctggccg cgctcgccgg
     7381 gcgtggccag cgccgcctgg ccttcgaggt agaaatgctc ctgcccgccc atctcgagcg
     7441 tgcccgacaa gcggtggcgc gcccgctcca gagcggcatc ggcgtcgccg cgcgccatgc
     7501 gctgcgcggg ctcgagatcg gaaccggcgt cgaaggcgtc gccgatggtg accagggcgg
     7561 cgagcggctc cgcctcgacc ctggccagcc gggccgcgat ccgcgccgcc cgggcggagg
     7621 tcgcggcgac gacgaagagg ggctggccgg gatagaggat ctcgtcctcg gcgaagagct
     7681 tgtcgtcgtg gaagaccggg ccgacattgt tctcgccggg gatgtcggcc gcggtgaaga
     7741 ccgccacgac gcccggcgcg gcgcggacct cggacagttc aaggcccgcc aggcgcgaat
     7801 ggccgtcggc ggcgaggccg aaggcgaggt ggagcgttcc cggcgcctcc ggaatatcgt
     7861 cgacataggc cgcggcgccg gcgacatgga ggtcggcgct gtcgtgcgga agcggccggt
     7921 tcacggcgcc cgcgcgcggc gacggcgagg cgttccgatc agccatggag cacgctggcg
     7981 ggctcgctct gctccgccca gatccggcgc agcaggttgg cggcgacctt gcgccggtag
     8041 gcggcggagc cgcgcaggtc gtcgagcggc gtgtagtcgt ccgccagagc ctgcgccgcc
     8101 gcctcgaccg tcgcctccgt ccaggctcga ccgatcaacg cggcttcgca gccgggcgcc
     8161 cgggcgggga tgccggccat tccgccgaac gcgacccggg cctcgcggac gatgccgtcg
     8221 gcgatgcgca gcacgaacgc gccgcagacc gcggatatgt cgctgtcgaa ccgcttggag
     8281 agcttgacga tccggatcag cgccgaggcc ggggggcgga agatccgaac gctctcgacg
     8341 aactcgccgg gcgcgaggtc ctgctggccg tagctcagga agaaattctc gagcgcgacg
     8401 gtgcgccgct cggatccgcg ccgcaggacg agctccgcgc cgatcgcgat cagggccggc
     8461 gccatgtcgc cgatcgggga gccgttggcg atgttgccgc cgatcgtccc ggcgttgcgg
     8521 acctggaccc cgccgatccg gcggatcagc tcgcccaggt cgggatggag gccggccagc
     8581 acctcgcggg cctcggagta acggacggcg gcgcccagtg tgacgtcgtc gggcctctcc
     8641 tcgatccgcc tcaggtcggc gatgtcgccg atgaacaccg tcgggccgag gtcgcgaagc
     8701 tgcttggtga cccacaggcc gacgtcggtc gctccggcaa tgatgcgcgt ctcgggattg
     8761 gcgaggagca gctcggccag ctcgtcggcg ctggccggat gtccgtaggc cggccgaagc
     8821 gctcgaagcg ccgccacggt cgcctcgtcg ttgccggcgc ccggcggcac cgcctcgccg
     8881 gcggcgagga tcgggccgta gccggtgcag cggcacaggt tgccggcgat gacgtccccg
     8941 accggcacgc cccgcgtgcc cagcgcgccg aggctccggc cgtagagcga catcacgaag
     9001 ccgggcgtgc agaagccgca ttgcgagccg tggcgctcga ccagcgcgcg ctggacggga
     9061 tgctcgcccc caaggctctc gacggtcctc agcgccttgc cgtcgagcat cggcaggaag
     9121 aggatgcagg aattgaccgc gcgccactgg acccggtccc cgtcgagctc gccgagcagc
     9181 acggtgcagg cgccgcagtc gccctccgcg cagccttcct tggtgccggt gcggcgcagc
     9241 ccgtagcgca gatggtcgag gacggtggcg gtcgggtcgc cgtcctctat ctcgatgacc
     9301 cggtcgtcga ggaggaagcg gatcatcgcg ggctccccgc ggcgatccag gcgcccagcg
     9361 tctgccgctc ggcctgggtc atgccggtgg gattgcccag cggcatcaca tgggaatcga
     9421 ccgccaccgc cttgatccgc gccgacgcgg cccgcacccg ctcccagctg tccagggcga
     9481 cgcccgccgg cggcggatcg gcgccgtggc agcccgcgca atgggtggcc aggatcggct
     9541 cgatctgggc gaaggcggga gtcccggccc ggccgggcgc ggccgccctc cgctcgagcg
     9601 tgacgtagct gacggtggcg agcgccaggg ccgcggcgac ggcgctcgtc gggcccgtcg
     9661 tccgtccgcg gccgcggcgg ttgaagacgt ggcggacggc tattccggtc agccccagca
     9721 gggccagcac cagccagggc cgctgcgccc cgtaggtcat cggatagtgc gggctgatca
     9781 tgatgaacag cacggggagg gtcatgtaat tgttgtgcag cgaccgctgc ttggccgccg
     9841 cgccgagcgc cgggtcgggg gtccgcccgg cgatcaggtc ggccacgacc ctgcgctggt
     9901 tggggatgat gacgaagaac acattgccga ccatcgccgt gccgacgatc gctccgacgt
     9961 gcagataggc cgcgcgcgag ctgaacagat ggtcgagcag gaaggcggcc cagaccaggc
    10021 agacgaacca gaaggcgccg agcagcaggt cgttcgagcc cagcggcgag cggcacaggc
    10081 cgtcatagac cagccagccg agcgccagcg ccgccaggcc gacggcgatc gccgcgcccg
    10141 ggctcatcat gaccttcgcc ggatcgatca ggaagctctg cgcgccgaca taatagatca
    10201 gcaccagcag cgagaagccg ctgatccagg tgaaataggc ctcccatttg aaccagtgca
    10261 gccgctcggg catgtggctc ggagcgacct ggtatttccg gttgtgatag aagccgccgc
    10321 cgtggaccga ccataattcg cccgaggcgt cgccctccgc cggcttcacc agatgattgt
    10381 cgagccagac gaaatagaag ctcgatccga tccaggcgat gcccgcggtc agatgcagcc
    10441 atcttatcgc gaggttgagc cattcgccga ggtcgacgcc catgctcagc tgccccggta
    10501 ggtcgaatag ccgtagggcg agacgagcag cggcacgtgg taatggccgc cgtcggccgc
    10561 gatcccgaag tcgacggtca cctcgccgag gaagggcgga tcgggcagca cgacgcccag
    10621 ggctcggaaa tagtccgcca cgccgaagcg cagcgcatag cgccccggcg ccagccccgc
    10681 cggcgccaga tccgggcagc ggccgtcggc attggtctcg ccccgcgcca tcactccgtc
    10741 gggcccgctg agcaccaccg ccattccggc cgccggccgt ccgctcatcg tgtcgagcac
    10801 gtgggtggag agcgagctca tgccctgtcc tccagccgca gccggacgat ctcgccgatc
    10861 tgcccgatcg cggtggcgat ctcgctgtcg cggtcgttgg cgagccggcg ttccatcgcg
    10921 gcgaggatgc ccgccttgtc ggtcagacgc acgcagatga tgaaggggaa gccgaatttc
    10981 tcgcgatagg cggcgttgag cgcgtggaag cgctcgaatt ccgccggggt gaggcggtcg
    11041 agccccgcgc tcgcctgctc ggccgcgctg gcctcggtca ggcggccgtc gaccgcggcc
    11101 ttgccggcga gctcgggatg ggcccggatc agcgccagct gctcctcggc cgaggcgtcg
    11161 cggaccacgg ccatcaggtc ggcatgccgg tcgcccgacg agggacgagc gtcggcgcgc
    11221 gcctcaaccc agggcgaatg ctcgaacagc gcgctcaccg cgccacccgc ccgatcagcc
    11281 tcagccggct cacgccgccg tccgggacga tgttgagccg cacgaagcgc accggcccga
    11341 gctcggcgag gctgtcgcgg aagacgtgga ccttgtccgc ctcgagcctc gccggcggca
    11401 tcagcagcgg ccagcgctcc gaggcggcgg cgatctcctt gggcgagccg ggcgcctcgg
    11461 ccgcggcctg gaggaagcag cggtccggat aattgccctt gaaatgggcg gtgtcgacga
    11521 tggcctgctc gatccgcccc ggcgcgccca gcttcagcac cgcccagtcg tgccccggct
    11581 cgcgccgccg ccgggtctcc cagccgtcgc ccatgttgac gccgcgcccc ggcgcggtga
    11641 ggttctcgac ccgcccgtaa tgctcgtcat tggcgatgat gccgcgcccg ccgttctcca
    11701 tcgccagcag gtcgagccgc tcgtccgggc cgaccctgga ccagtccttg gcgacgctgc
    11761 cccagacccg cagccgcgcc actccgccgt cggggaagat gttgagccgg acatgggtcg
    11821 ccggagcgtc atgctcgacc gggacgtaga tgcggtcgtc gcccttcagc gcgaggcgct
    11881 ccgtcaccgc cgtccagccc agccgctcgt cggggatctc ggagtcgctc cggcagacct
    11941 cgatctgcgc ggcgggcgga tagttgccgg tgaaatgccg ggtgtcgatc tcgaagccgg
    12001 cgatccgccc cggcaccccc agccggacga cgcaccagtc gtggccgggg atgcgcttgc
    12061 ggcggctctc ccagccgtcc atccacttgc cgttcgaatc ggatgtgccc ggtatgaaga
    12121 ccggctcggc gggatcgatc agccgcgcct tgtcggcgaa gaaatcgtcg gtggcgtaga
    12181 cgacccgggc ccccagccgc ggctgggcca gatcgatatg atggcggcga agctcctcga
    12241 gcatgatcat atcgtcggac acggatgacc atttctccgt tcgggctgag cttgtcgaag
    12301 cccgttcctt tcctcgctcg ggcaaaggga aatagagggc ttcgacaggt tcagcccgaa
    12361 cggttgtctg ctgctcaacg gaagtcgtcc tccacctgat gaggatgggt ctcgcgccag
    12421 tggcgggcga tgtcgatccg ccggcacagc cagacatccg catggccgag cgcgtggcgg
    12481 aggaagcgct cgagcccggc gagcttgccg ggccggccgg cgagccggca gtggagcccg
    12541 accgacatca tcttcggcct ctcctcccct tccgcataga ggatgtcgaa cgcatcgcgc
    12601 agataggcga agaactggtc ccccgtgtgc atgttcgccg acgcgaagcg catgtcgttc
    12661 gtctccagcg agtaaggcac gatcaggtgg ctccggccct cgacccgggc ccagaacggc
    12721 aggtcgtccg aataatcgtc ggcgtcgtag aggaagccgc cctcctccgc cacgatccgg
    12781 cgggtattgg ggctggtccg gccggtatac cagcccagcg gccgctcccc ggtcagctgc
    12841 cggtggacct cgatcgcccg ctccatgtcg gcgcactcct gggcctccgg cacccgctga
    12901 tagttgatcc accgccagcc gtgcgaggcg atctcgtgcc cggcctccag gaacgcttcg
    12961 acgacggccg gatggcgctg catcgccagc gcgaccccga acacggtgac gggcacccca
    13021 tagcgctcga acagccgaag cagccgccag acgccggccc gcgacccgta ttcgtagatc
//
LOCUS       DASVQZ010000431         7198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_187561, whole genome shotgun sequence.
ACCESSION   DASVQZ010000431 DASVQZ010000000
VERSION     DASVQZ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187561"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(545..1384)
                     /locus_tag="VF547_12130"
     CDS             complement(545..1384)
                     /locus_tag="VF547_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8263613.1"
                     /translation="MATAAKTETHDYDAGAIMGGLYGDGIIALQGAFSRPWVERMRDD
                     IRLLFEEARQVPGGALPRGPHRWYVEVHPERISGFVAIATHPWFVAVCEAVLGPDYRI
                     VEVGFDIPFPGAADQPWHRDFPAPEATTKGRRLNSLAFNLTAVDTRPEHGAFEIAPGT
                     QWDDLSGCKDGMFPPKDWWPRYISRKVAKLAQMGDISARSALTIHRGTANRSDEWRPV
                     LVVGVDAPDATNAGHHDLQVTRGYLESLPPRVRDHLTYRLTDALKPVIQNHVIGGLLE
                     PDY"
     gene            1464..2882
                     /locus_tag="VF547_12135"
     CDS             1464..2882
                     /locus_tag="VF547_12135"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEX8263614.1"
                     /translation="MSENARFAFEVEAVEEGEGTRFEVRARGCAQPRAYRVPGGEQAD
                     YAGFYRELAHDWGTRVPHAFAEPSEAPEAEVEWRPLLTDNLSPRILAGYGDPAVLKTD
                     RGYVLVATSNDAPDAFPILRSDDLEHWHHEGFVFPEGQAPAWAATGRRVADFWAPEMA
                     RMGDEYWLVYTARQASNGLSIGLAKSRSPFGPWRDIGRPLVSRHLVNTTGQPDDPARP
                     LLSGGVIDSHIFIDFDGRPWLFWKADTNGVWPRPLAALLRDRPELIDRLFEREEDRRT
                     AAFAAAIVAWANTRRPMERFFLMQPLIEAALANWSPVRAALEQSGLAAAILEAMRTPI
                     YAQPLADDGESLVGEPTIVLANDLDWEGHLIEGPWVTRQQGRYWLFYAGNDFSTPAYG
                     IGVAVADHVLGPYVKQAEPLLRSTRRWTAPGHASVAPGPDGEPQLFFHAFHPGTGGYN
                     VFRALLTARLSFSSSGVAILPA"
     gene            complement(2895..3734)
                     /locus_tag="VF547_12140"
     CDS             complement(2895..3734)
                     /locus_tag="VF547_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8263615.1"
                     /translation="MIDSTPFYLMSDHGEGERLPLRHGCVVLSRLPGGRSAVRALAPS
                     VKFVLAGEEVYKIDGRSRAVKPGRFLLVEAGTEFEVSTPRPDETVGLCVYLGVTSAPT
                     PERGPLSRVIEGCGAEPLAPLLSRYARLLASRPEAGPRLAPKIVQEVSAGAEDYLSTF
                     ASRLERLSSLKRSTRIETLQRVERARAFIHDHRGAPLTLDAIAAQAALSRFHLSRSFA
                     EVHGLPPLAYHRRLRLEGAARLLREGAASPTELAERLGYGSLSAFTRAFRQAYGVPPS
                     RAQ"
     gene            3857..4633
                     /gene="xth"
                     /locus_tag="VF547_12145"
     CDS             3857..4633
                     /gene="xth"
                     /locus_tag="VF547_12145"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8263616.1"
                     /translation="MRIVTFNVNGVNGRLPLLLEWLAEAQPDAVCLQELKAPQDKFPA
                     RAIEKAGYGAIWHGQQRWNGVAILARDCEPVETCRGLPGDPDDSQSRYIEAAVCGVLI
                     GGLYAPNGNPHPGPKFDYKLAWNERLIARASELVALDEPVVLAGDYNIIPTPTAIDAY
                     KPERWMDDALMQPEPQAQYRRLVAQGWTDALRHFHPDEPIFTFWDYWRNAFARNAGIR
                     IDHLLLNKPAAKRLKAAGVDRDLRAREKASDHAPTWVELE"
     gene            4768..5028
                     /locus_tag="VF547_12150"
     CDS             4768..5028
                     /locus_tag="VF547_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263617.1"
                     /translation="MLKKALRLFVIKTRFEAWAIIYALAVGAVTRGEIYLAQYPGPAG
                     WMLFACCTGAVFMAGAKILDSTRGASCRRSTLHRVIARARTF"
     gene            5215..5358
                     /locus_tag="VF547_12155"
     CDS             5215..5358
                     /locus_tag="VF547_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263618.1"
                     /translation="MFNQKIRESVAAAMLAIVLSTTAIGAAVGPARAIETDTSLQAPV
                     QVA"
     gene            5507..6187
                     /gene="pspA"
                     /locus_tag="VF547_12160"
     CDS             5507..6187
                     /gene="pspA"
                     /locus_tag="VF547_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEX8263619.1"
                     /translation="MSIFSRTRDIVAANFSDLLDKAEDPAKMIRMIILEMEETLVEVR
                     ASAARTIADQQEMRRQILRLEKLQASWTEKAELALSKGREDLAHGALTEKQKAADMAD
                     SLRAEIEALDDSMRASEADIAKLQGKLREARMRQTATRSRLESANQRARLREMTAGPK
                     VDEAFSRFDLLERQVDHAEGRMEALALAAPPKTLDEEIAELRNAEKVEADLAAMKARL
                     AGAAEKGA"
     gene            6192..6377
                     /locus_tag="VF547_12165"
     CDS             6192..6377
                     /locus_tag="VF547_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263620.1"
                     /translation="MEAISPVFAALGVSGFDALDDLAGWLDGGFEPATAPGQAARVGR
                     WDGRVPRTPGLSAEREG"
     gene            6381..6674
                     /gene="pspB"
                     /locus_tag="VF547_12170"
     CDS             6381..6674
                     /gene="pspB"
                     /locus_tag="VF547_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HEX8263621.1"
                     /translation="MEPVLPLFGVFMVFVAFPWIILHYVTQWKKSATLTIEDERLLDE
                     LHELARRLDDRVCTVERIMTAENPNWRSLSCDPAATRIEDLRRDNIETIRRER"
     gene            6671..7024
                     /locus_tag="VF547_12175"
     CDS             6671..7024
                     /locus_tag="VF547_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX8263622.1"
                     /translation="MKKRFYLDKADGRIFGVCSGIARHMGWDPTFVRVGVVLVTLLGA
                     FPWTLIAYGAVAMIAKNRPLGGRHEEDAPVRVAKMSTFELNRSMRDIDRRMAEVETYV
                     TAQNTGLAREIESLR"
BASE COUNT         1185 a         2513 c         2376 g         1124 t
ORIGIN      
        1 agatgtcgtc cccacgcatc ttcacttccg gctccgcctc ccgggggagg ctgttgcgca
       61 cttccgtcaa ccgctcgatc aacgcgtcga gcgaggtgac gtccttgacc tcgataaaat
      121 ccttgactcg tctactcata acaaacccct cccagctctc cccgggacgt aactaacttg
      181 cctcggaatc gcggtgcaag cttaatctac atttacccac aggagagttg cagacgcctg
      241 aacgatcttg ttgcgtggtt cagccgcggc cgcggcggcc atgccgccgg cactcgcgcg
      301 aaccgcccca ctcgcgcccg gccggccgct cccgcaacgt cataatgcgc ctgcaatgct
      361 gcgcggtaag tagcggtaaa aaagcggata atcggcgccc gcaacgaagc catgcggggc
      421 tggcgcgctc ccgaatcccg gctggtgcgt tcgctgcccg gcccccccgt cggcgcctcc
      481 tgcccgccgt ggccgcagag tcacacctgc gcgaaattcg ttatcggatt cccgatcgcg
      541 gggctcagta atccggctcg agcagaccgc cgatcacatg gttctggatg accggcttca
      601 gcgcgtccgt cagcctgtag gtaaggtggt cgcgcacccg gggcggcagc gattcgagat
      661 agccgcgggt cacctgcagg tcgtggtggc cggcattggt cgcgtcgggc gcgtcgactc
      721 cgacgaccag caccggtcgc cattcgtcgg agcggttggc ggtgccgcgg tggatggtga
      781 gcgccgagcg ggcgctgatg tcgcccatct gggcgagctt ggcgaccttc cgggagatgt
      841 agcggggcca ccagtccttc ggcgggaaca tgccgtcctt gcagccgctc agatcgtccc
      901 actgcgtccc cggggcgatc tcgaacgcgc cgtgctcggg ccgcgtgtcg accgcggtga
      961 ggttgaaagc gagcgagttg agccgccggc ccttcgtcgt cgcttccggc gccgggaagt
     1021 cgcggtgcca gggctggtcc gccgcgccgg ggaaggggat gtcgaagccg acctcgacga
     1081 tccggtagtc cgggccgagc accgcctcgc acacggcgac gaaccaggga tgggtggcga
     1141 tggcgacgaa gccgcttatc cgttccggat ggacctccac gtaccagcgg tgcggcccgc
     1201 gcggcagagc gccgccgggc acctgccgcg cctcctcgaa cagcagccgg atgtcgtcgc
     1261 gcatccgttc gacccaaggc cgcgagaagg ccccctggag cgcgatgatg ccgtcgccgt
     1321 agaggccgcc catgatcgcc ccggcatcgt aatcgtgagt ctcggtcttc gcggccgtcg
     1381 ccatcgctga atcaccccgc ccctgaagcc ttcttgaatt aatccaattg tatgatgaat
     1441 gcgaggccgc taacgggccg ccgatgtcgg aaaatgcacg attcgccttc gaggtcgagg
     1501 ccgtcgaaga aggggagggg actcgcttcg aggtgcgggc ccgcggctgc gcgcaaccac
     1561 gcgcctatcg agtcccgggc ggggagcagg ccgattacgc gggtttctac cgcgagctcg
     1621 cgcacgactg gggcacccgc gtcccccatg ccttcgccga gccgtcggag gcgccggagg
     1681 ccgaggtcga atggcggccg ctcctcaccg acaacctgtc gccgcggatc ctcgccggct
     1741 atggggatcc cgccgtgctc aagaccgacc ggggctatgt tctcgtcgcc acctccaacg
     1801 atgctccgga tgccttcccg atcctgcgtt cggacgacct cgagcattgg caccacgagg
     1861 ggttcgtgtt cccggagggc caggcgccgg cctgggccgc gaccgggcga cgggtcgccg
     1921 atttctgggc gcccgaaatg gcccggatgg gcgacgaata ctggctcgtc tacaccgctc
     1981 gccaggcgtc gaacggcctc tcgatcgggc tcgccaagag ccgcagccca ttcggtccat
     2041 ggcgcgatat cggtcgaccg ctcgtgtccc gccacctggt caacacgacc ggccagcccg
     2101 acgatcccgc caggccgctg ctcagcggcg gagtgatcga ctcccacatc ttcatcgatt
     2161 tcgacggccg gccctggctg ttctggaagg ctgataccaa cggtgtctgg ccgcgcccgc
     2221 tcgccgcgct gctgcgcgac cggccggagc tgatcgaccg cctgttcgag cgcgaggagg
     2281 accggcgcac cgccgccttc gccgcggcga tcgtcgcctg ggcgaacacc cgccggccga
     2341 tggagcgttt cttcctgatg cagccgctga tcgaggcggc gctggccaat tggtccccgg
     2401 tcagggcggc gctcgagcag agcggccttg ccgccgccat cctcgaggcg atgaggacgc
     2461 cgatctatgc tcagccgctc gcggacgacg gcgaatcgct ggtcggcgag cccacaatcg
     2521 tgctcgccaa cgatctcgac tgggaaggcc atctcatcga gggcccctgg gtgacgcgcc
     2581 agcagggccg ctactggctc ttctacgccg gcaacgactt ctcgactccc gcctatggga
     2641 tcggggtcgc ggtcgccgac catgtcctgg ggccctacgt caagcaggcc gagccgctgc
     2701 ttcgctcgac gcggcgctgg actgcgccgg gccacgcttc ggtggcgccg ggacccgacg
     2761 gcgagccgca gctcttcttc catgccttcc atcctggcac cggcggctac aacgtgttcc
     2821 gggcgctgct caccgcgcgg ctatcgttca gcagttccgg agtcgccatt ctgcccgcgt
     2881 gaatcccgct tcgattattg agcgcggctc gggggcaccc cgtaggcctg gcggaaggcg
     2941 cgggtgaagg cgctcagcga cccgtagccg agccgctcgg cgagttccgt cggcgaagcg
     3001 gcgccttccc gcagcaagcg cgccgccccc tccaggcgaa gccggcggtg ataggccagc
     3061 ggcggcaggc cgtgcacttc ggcgaagctc cggctgaggt ggaagcgcga caaggctgcc
     3121 tgcgccgcga tcgcgtcgag ggtcaggggc gcacctcgat ggtcgtggat gaaggcccga
     3181 gcgcgctcga cccgctgcag cgtctcgatc cgggtcgacc gcttgaggct ggacagccgc
     3241 tcgagccggc tggcgaaggt cgagagataa tcctccgcgc cggcgctgac ctcctggacg
     3301 atcttcggcg ccagccgggg cccggcctcg gggcgcgacg ccagcagccg ggcgtagcgc
     3361 gaaaggagcg gagcgagcgg ctcggcgccg cagccctcga tcactcggct gagcgggcct
     3421 cgctcgggcg tcggcgccga cgtgacgccg agatagacgc acaggcccac cgtctcgtcc
     3481 ggacgcggcg ttgacacctc gaactccgtc ccggcctcga ccagaaggaa gcgcccgggc
     3541 ttcaccgcgc gcgagcggcc gtcgatctta taaacctcct ccccagccag cacgaacttc
     3601 accgagggcg ccagcgcccg aacggcgctt cgcccgcctg ggagccgcga gagcaccacg
     3661 cagccgtgcc gaagcggcag ccgctccccc tcgccatggt ccgacatcag gtagaaaggc
     3721 gtcgagtcga tcacaaatgt aatcgcaagc aaaatcgatg ccacgcaagg ctttggcgga
     3781 tttccgccat ttcccggcgc ttgacactta ttgctcatgt ccggaaccgg gcgtcgccgt
     3841 gcgctttcgg acgcccgtga ggatcgtcac cttcaacgtc aacggggtca acggccgcct
     3901 gccgttgctg ctcgaatggc tcgccgaggc tcagccggac gcggtctgcc tgcaggagct
     3961 caaggcgccg caggacaagt tcccggccag ggcgatcgag aaggccggat atggcgcgat
     4021 ctggcacggc cagcagcgct ggaacggagt cgccatcctg gcccgggatt gcgagccggt
     4081 ggaaacctgc cgcggccttc cgggagatcc ggacgacagc cagagccgct acatcgaggc
     4141 cgccgtctgc ggcgttctga tcggcggcct ctacgccccc aacggcaatc cgcacccggg
     4201 accaaagttc gactacaagc tcgcctggaa cgaacgtctg atcgcccgtg cctcagagct
     4261 ggttgcgctg gacgaacccg tcgtgctggc cggcgactac aacatcatcc cgactcccac
     4321 cgcaatcgat gcctacaagc cggagcggtg gatggacgat gcgctgatgc agcccgagcc
     4381 ccaggcgcaa tatcgccggc tcgttgccca gggatggacc gatgcgcttc ggcacttcca
     4441 tcccgacgag ccgatcttca ccttctggga ttattggcgc aacgccttcg cccgcaacgc
     4501 cgggatccgg atcgaccatc tgctgctcaa caagccggcg gcgaagcggc tgaaggcggc
     4561 gggcgtcgac cgcgaccttc gcgcccgcga gaaagcgagc gaccacgcgc ccacctgggt
     4621 cgagctcgaa taggggagag ccgtgggacg gtcaggcaac tgtccctcct gtcccgcctg
     4681 cgtcgggtcc gtttacactt tcggccgcgc ccaggaccaa gaatggcgga aatccgccat
     4741 tggcccggct cttgctgctg aacggacatg ctgaagaagg cgttaagact tttcgtcatc
     4801 aagacccgct tcgaagcctg ggcgatcatc tacgccctgg ccgtcggagc ggtcacccgc
     4861 ggcgagatct acctcgccca atatcccggg ccggccggct ggatgctgtt cgcgtgctgc
     4921 accggcgcgg tgttcatggc cggcgccaag atcctcgact cgacccgcgg cgcttcctgc
     4981 cggcgctcga cgctgcaccg ggtgatcgcg cgggcgagga ccttctaggc tgcgacaagc
     5041 ttcccaaccc ttggcggaaa atcccaatcc gcggcgcggg gaatccggtc cggttgtctt
     5101 ttaatcgctc attctccgca gttttccgcc gttttctgaa cttggcacgc gccttgtaac
     5161 cttgccgaca accgctgcga agcggggcag caacaaggtt caaggagcag tccgatgttc
     5221 aatcagaaga tcagggaaag cgtcgcggcg gccatgctgg cgatcgtgct ttcgacgacg
     5281 gcgatcggcg ccgcggtcgg cccggcgcgg gcgatcgaga ccgatacttc cctccaggcc
     5341 cccgtccagg tcgcctgatc ccatgccgga gacgatcctc gtgcccgccg acaagctgac
     5401 tcggatcccg ccgcgccccg gcggcccgcg tcgccccgaa ggctaccgcg gccagcgcag
     5461 cgagcgcccg gcccggtccc ccatctcgct gatggagcta ctgccgatga gcatcttctc
     5521 ccgcacccgc gacatcgtcg ccgccaattt ctccgacctc ctcgacaagg ccgaggaccc
     5581 cgcgaagatg atccggatga tcatcctcga gatggaggag accctcgtcg aggtgcgcgc
     5641 gtcggccgcc cggacgatcg ccgaccagca ggaaatgcgc cgccagatcc tgcgtctgga
     5701 gaagctccag gcgagctgga ccgagaaggc cgagctggcc ctctcgaaag gccgcgagga
     5761 cctcgcccac ggcgcgctca ccgagaagca gaaggccgcc gacatggccg acagcctcag
     5821 ggcggagatc gaggcgctcg acgactccat gcgcgcctcg gaggccgaca tcgccaagct
     5881 gcagggcaag ctgcgggaag cccgcatgcg gcagaccgcc acccgcagcc ggctcgagag
     5941 cgccaaccag cgcgcccgcc ttcgcgagat gacggcggga cccaaggtcg acgaggcctt
     6001 ctcacgcttc gatctgctcg agcgccaggt ggatcatgcc gaaggccgga tggaggccct
     6061 cgccctcgcc gctccgccga agaccctgga cgaagagatt gccgagctcc gcaacgccga
     6121 gaaggtcgaa gcggacctcg ccgcaatgaa ggcgcgcctc gccggcgccg ccgagaaagg
     6181 ggcctgagac gatggaagcg atttcgcccg tattcgccgc gctgggagtc agcggcttcg
     6241 acgccctgga cgacctggcc ggttggctcg acggcggttt cgagcccgcg acggccccgg
     6301 ggcaggccgc ccgggtcggc cgttgggacg gtcgtgtccc gcggaccccg ggcctctccg
     6361 ccgagcggga gggctgagcg atggagccgg tccttcccct gttcggagtc ttcatggtct
     6421 tcgtcgcctt tccctggatc attcttcact acgtgaccca gtggaagaag tccgcgacgc
     6481 tgacgatcga ggacgagcgg ctgctcgacg agcttcacga gctcgcccga cgcctcgacg
     6541 accgggtctg caccgtcgag cggatcatga ccgcggaaaa tcccaattgg cgaagcctct
     6601 catgcgaccc ggccgcgacc cggatcgagg accttcgccg tgacaatatc gaaacgatca
     6661 ggagggaaag atgaaaaagc gcttctatct ggacaaggcc gacggccgca tcttcggtgt
     6721 gtgctcgggc atcgccaggc acatgggttg ggacccgacc ttcgtccgcg tcggagtggt
     6781 gctggtcacc ctgctgggcg ccttcccctg gaccctgatc gcctacggcg cggtggcgat
     6841 gatcgccaag aatcgcccgc tgggcggccg ccatgaggaa gacgctccgg tgcgcgtggc
     6901 caagatgtcg accttcgagc tcaaccgctc gatgcgcgac atcgaccgcc gcatggccga
     6961 ggtcgagacc tatgtgaccg cgcagaacac cggcctcgcc cgcgagatcg aaagcctgcg
     7021 ctgagcctca agagaagaaa cgggaggtag tcatgagcga accgatgacg atggtggcga
     7081 tggcgggctt cggcctcgcg gcgctcggca tcatgctctt cgctgcgctg aagggctggc
     7141 agggctggct cgacctgaag cggctggagc tgcagagcgg aacggtccgg ccggcaag
//
LOCUS       DASVQZ010000432         4459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_765286, whole genome shotgun sequence.
ACCESSION   DASVQZ010000432 DASVQZ010000000
VERSION     DASVQZ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765286"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VF547_12180"
     CDS             <1..722
                     /locus_tag="VF547_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263623.1"
                     /translation="VLDVAPGDANTNFLAGLAAAGLNDLRGSRKYYERAVKADKELVP
                     ARRELGVTYVKLGDKAKADAQLAALKAMQEKCGGACAKAAEIGGAIEALTAAIGTPPQ
                     ARLETRPSLLFASAAGGDRAYLDAIALINEGRYESAIEGLNAARATFGPHPDVLTYLG
                     FAHRKLGRLNAAEAYYRSALAAVPDHRGATEYYGELMVERGDLAGAERMLAKLEASCD
                     FGCAEADELRRWIQAARSRAS"
     gene            689..1615
                     /locus_tag="VF547_12185"
     CDS             689..1615
                     /locus_tag="VF547_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263624.1"
                     /translation="MDPGRAFARFLSALPFALLPAAAADGLRLPVREARWLAPAALHR
                     GLAREPAECFARPADPAERRRAWIGRIAFRTPLLLGGQAARSGLSCASCHLNGRGNPD
                     FQFPGLSGAPGTADVTASLMSSHRGDGSFNPRPIPDLAGPRAALNIPQERGSRALEAF
                     IRGLVVEEFDGAPPPAPVLDGLAAYVRALRPEACRSGWTRVRLASRLALVEEAVRLAS
                     AADGETRRLLLGAARSQLGAIDERFAIPGGEPARTLLRDADRQLAALRAGQGDLRQWR
                     RRWPAWKRGLLRLERSSLFDSRRLRAALEGRG"
     gene            complement(1612..2598)
                     /locus_tag="VF547_12190"
     CDS             complement(1612..2598)
                     /locus_tag="VF547_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263625.1"
                     /translation="MTSQADVGETEAPADEAPLTFSAPYLILDDFLPLDEARAMRRQI
                     DGHFAEPHRHTPDRHQIWNYWHVPGLYTYLRTTPDKVIERALVARFVQALTVWAEYML
                     GLGHVTWPYLSLYVDGCEQKLHNDSTNGRFGFVYSLTWDERKTMGGETIVLKEGDLFR
                     DNMGRASAGAGLLDLIEPKFNRLTLFDDRMPHGVQRVEGSMNPVEGRFVLHGHISENG
                     PSADGPLDAVAVARSVYDGLGPVLSEVQRMPVLHHGPLVLRLAIEPSGTVSRVDRLVD
                     RLARSDGGSAGALASQVVALVHRMRFPQAPAATEATVPLMFGGNLPWMRPTG"
     gene            2715..3023
                     /locus_tag="VF547_12195"
     CDS             2715..3023
                     /locus_tag="VF547_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263626.1"
                     /translation="MTRLILVAAAGLLLATANTPPTTEGELSSVVAAPAPEQSATPAV
                     HAKHAKPARPYKPAHAAAGAYPRCSATVRDRCQQGRGYAARPARQREIQYAMRAGERG
                     "
     gene            complement(3167..>4459)
                     /locus_tag="VF547_12200"
     CDS             complement(3167..>4459)
                     /locus_tag="VF547_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX8263627.1"
                     /translation="RLAPASVPALLEQLLGAVAVRPPYGQHPRLFIWGLLEARLQSAD
                     LVILGGLNEGVWPQLPVPDPWLAPRVRHELGLPSLERRIGLAAHDFAVALGGREVLVT
                     RARRDSRSPAIASRFWLRLEAMTAGITRSPRHKSWARELDRPAGFHPAARPAPRPPAS
                     LRPRQIAVTDVDRLKADPFAFYARKMLAFAPLDPIDADPSAAWRGSAVHKTLEAWMKE
                     DDCEPSRLRPRAEALLRDAAAHPLMRALWQPRLLEAIDWIAAEMAKNFAAGRRPVAAE
                     AKGTCELEGVKLYGEADRIDRLADGRLAIVDYKTGKPPSAKAVAEGYSLQLGLIGLIA
                     ARGGFDGIEGVPACFEYWSLARDRTGKLGYVASPVGGRTGIDVADFTTMATRNFLAAA
                     MRWLIGDEPFTAKLHPEHAPYGDYDQLMRLDEWYGREA"
BASE COUNT          641 a         1615 c         1602 g          601 t
ORIGIN      
        1 gcgtgctcga cgtcgcgccg ggcgacgcca acaccaattt cctcgccggc ctcgccgccg
       61 ccggactcaa cgacctcagg ggctcgcgca aatattacga gcgcgcggtg aaggccgaca
      121 aggagctcgt gccggcgcgc cgcgagctcg gggtcaccta cgtaaagctc ggcgacaagg
      181 cgaaggccga cgctcagctc gccgccctca aggcgatgca ggaaaaatgc ggcggggcct
      241 gcgccaaggc ggcggagatc ggcggagcga tcgaggcgct gacggccgcg atcggcactc
      301 cgccgcaggc ccgtctcgag acccggccca gcctcctgtt tgcaagcgcc gcgggcggcg
      361 accgcgccta tctcgacgcg atcgccctga tcaacgaggg acggtacgag tcggcgatcg
      421 aggggctgaa cgcggcccgc gcgaccttcg gcccgcatcc cgacgtcctc acctatctcg
      481 gcttcgccca ccgcaagctc ggccgcctca acgccgcgga ggcctattac cgctcggccc
      541 tggccgcggt ccccgaccat cgcggagcga ccgaatatta tggcgagctg atggtggagc
      601 gcggcgacct cgccggcgcc gagcggatgc tggcgaagct ggaggccagc tgcgacttcg
      661 gctgcgccga ggccgacgag ctgcggcgat ggatccaggc cgcgcgttcg cgcgcttcct
      721 gagcgccctt cccttcgccc tgctgcccgc ggcggccgcg gacggcctcc ggctgccggt
      781 gcgcgaggcg cgctggctgg cgcccgccgc cctccaccgg ggcctcgccc gcgagcccgc
      841 ggaatgcttc gcgaggcccg ccgatccggc cgagcgccgc cgggcctgga tcggccggat
      901 cgccttccgc acgccgctcc tgctcggagg ccaggcggcg cgctccgggc tgagctgcgc
      961 aagctgccac ctgaacggcc gtggcaatcc cgatttccag ttcccggggc tttccggcgc
     1021 tcccggaacc gccgacgtca ccgcctcgct gatgagcagc catcgcggcg acggcagctt
     1081 caatcccagg ccgatccccg atctggccgg gccgcgcgcc gcgctcaata tcccgcagga
     1141 gcgcggaagc cgcgcgctgg aggccttcat ccgtggtctc gtcgtcgagg agttcgacgg
     1201 ggcgccgccc ccggctccgg tgctcgacgg cctcgccgcc tacgtccgcg cgctgcggcc
     1261 ggaggcgtgc cggtccggct ggacccgagt caggctcgcc tcgcgcctcg ccctggtcga
     1321 ggaagcggtc cggctggcct cggcggccga tggggagacg aggcgcctgc tcctcggcgc
     1381 ggcgcgctcg cagctcggcg cgatcgacga gcgcttcgcg atccccggcg gcgagccggc
     1441 gcggacgctg ttgcgggacg ccgatcggca gctcgccgcg ctgcgcgccg gccaaggcga
     1501 ccttcgccaa tggcggcggc gctggccggc gtggaagcgc ggcctgctcc ggctcgagcg
     1561 cagttcgctg ttcgattccc gccgcctgcg cgcggcgctc gaggggcggg gctagccggt
     1621 cgggcgcatc caggggaggt tgccgccgaa catgagcggc accgtcgcct ccgtcgccgc
     1681 cggcgcctgc gggaagcgca tccggtggac gagcgcgacg acctggctgg cgagggctcc
     1741 ggcgctgcct ccgtccgaac gggcgagccg gtcgaccagc cggtccaccc gcgacaccgt
     1801 gccggagggc tcgatcgcga gcctgagcac gagcggcccg tgatggagca cgggcatgcg
     1861 ctggacctcg gagagcaccg ggccgaggcc gtcatagacc gagcgcgcga cggcgacggc
     1921 atccagcggg ccgtcggccg agggcccgtt ctcgctgata tggccgtgaa ggacgaagcg
     1981 cccctcgacc gggttcatcg agccttcgac ccgctgcacg ccgtgcggca tgcggtcgtc
     2041 gaacagggtc aggcggttga acttcggctc gatcaggtcg agcagcccgg cgcccgccga
     2101 ggcccggccc atgttgtcgc ggaacaggtc gccttccttg agcacgatgg tctcgccccc
     2161 catcgtcttc cgctcgtccc aggtcagcga atagacgaag ccgaagcggc cgttggtcga
     2221 atcgttgtgg agcttctgct cgcagccgtc gacatagagg ctgagataag gccaggtgac
     2281 gtgccccagg cccagcatat attccgccca gacggtcagc gcctggacga agcgcgcgac
     2341 cagcgcgcgc tcgatcacct tgtccggagt ggtcctcaga taggtgtaga ggccgggcac
     2401 atgccagtaa ttccagatct ggtgacggtc gggcgtgtgg cggtgcggct cggcgaagtg
     2461 cccgtctatc tgacgccgca tcgccctcgc ctcgtcgagt ggcaggaagt cgtcgaggat
     2521 cagatagggc gcggaaaagg ttaacggggc ctcgtcggcc ggcgcttccg tctcgcccac
     2581 gtccgcttgt gacgtcatga cgcctcctgt agattgccgg agagtccagt gataagctgt
     2641 tgaggcgact tcacaagcgc cagggaagcg aagcgcggcg ccgagcattc gagccgccga
     2701 caaaggagag ccgaatgacc agactgatcc tcgtggccgc ggcgggtctg ctgctcgcca
     2761 ccgcgaacac cccgccgacg accgaaggcg agctttcctc ggtggtcgcc gcgccggcgc
     2821 ccgagcagtc cgccacgccg gccgtgcacg ccaagcacgc caagccggcg aggccctaca
     2881 agccggccca cgccgcggcg ggcgcctatc cgcgctgttc ggcgaccgtc cgcgaccgct
     2941 gccagcaggg ccgcggctat gccgcccgtc ccgcccgcca gcgcgagatc caatacgcca
     3001 tgcgggccgg cgagcgcggc taggcaggcc ccgcactaaa ttcctccccg gccttgccgg
     3061 agaccaggcg aagcctggtg gaggggtatt ttcacgcctc gaagaccctc caccgcgcct
     3121 cgcgcggtcc cccctccccc gggaatccgg gggaggaatt ccgaatctac gcctcccggc
     3181 cgtaccattc gtcgagccgc atcagctggt catagtcccc gtagggggcg tgctcggggt
     3241 gcagcttggc cgtgaagggc tcgtccccga tcagccagcg catcgcggcc gcgaggaagt
     3301 tgcgggtcgc catggtcgtg aaatcggcga cgtcgatgcc ggtgcggccc ccgaccggac
     3361 tggcgacata gccgagcttg ccggtccggt cgcgggccag ggaccaatat tcgaagcagg
     3421 cggggacgcc ctcgatgccg tcgaagccgc cgcgcgcggc gatcaggccg atgaggccga
     3481 gctgaagcga atagccttcc gccaccgcct tggcgctggg cggcttgccg gtcttgtagt
     3541 cgacgatggc gagtctgccg tccgccagac ggtcgatccg gtcggcctcg ccgtatagct
     3601 tcaccccttc cagctcgcag gtgcccttcg cctcggcggc gacgggccgg cgtccagcgg
     3661 cgaaattctt cgccatctcc gcggcgatcc agtcgatcgc ctcgagcagc cgcggctgcc
     3721 agagcgcgcg catcagcgga tgggcggcgg cgtcgcgcag cagcgcctcc gcccgagggc
     3781 gaagccgcga cggctcgcaa tcgtcctcct tcatccacgc ctcgagcgtc ttgtggaccg
     3841 cgcttccgcg ccaggcggcg ctcggatcgg cgtcgatcgg atcgagcgga gcgaaggcca
     3901 gcatcttgcg cgcgtagaag gcgaacggat cggccttgag ccgatcgacg tcggtcaccg
     3961 cgatctggcg cgggcgcaag gacgcgggcg gacgcggcgc ggggcgggcg gcggggtgga
     4021 agccggcggg ccggtcgagc tcgcgcgccc agctcttgtg ccgcggcgag cgggtgattc
     4081 ccgccgtcat cgcctccagc ctgagccaga agcgcgacgc gatggccggc gagcgggaat
     4141 cccggcgggc gcgggtgacg agcacttcgc ggccacccag ggcgacggcg aaatcgtggg
     4201 cggcaaggcc gatgcgccgc tccaggctgg gcaggccgag ctcgtggcga acgcgcgggg
     4261 cgagccacgg atccggcacc ggcagctgcg gccagacgcc ctcgttgagg ccgccgagga
     4321 tgacgagatc ggccgactgc agccgggcct cgagcagccc ccagatgaac aggcgcggat
     4381 gctggccgta gggcggccgg accgccaccg cgccgagcaa ttgctcgagc agcgccggaa
     4441 cgctcgccgg cgccaggcg
//
LOCUS       DASVQZ010000433         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_859279, whole genome shotgun sequence.
ACCESSION   DASVQZ010000433 DASVQZ010000000
VERSION     DASVQZ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859279"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF547_12205"
     CDS             complement(<1..336)
                     /locus_tag="VF547_12205"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX8263628.1"
                     /translation="MARLVALLRAVNVGGRKLPMAELRALCGELGWKRVETYIQSGNL
                     LFEAPGEAHRLEAELEKAIGDRFGLDVPAIVRTAGQWAAYVKANPFPELARDAPNRLQ
                     MVVSKAPPDP"
     gene            384..1016
                     /gene="gmk"
                     /locus_tag="VF547_12210"
     CDS             384..1016
                     /gene="gmk"
                     /locus_tag="VF547_12210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX8263629.1"
                     /translation="MEIASAIQRRGLLFILSSPSGAGKSTIARMLLASDDGVAMSVSA
                     TTRPKRSGEVDGRDYHFVDDAGFDSLIAGGHFLEWAHVFGHRYGTLKSEVLKTIEGGR
                     DVLLDIDWQGTQQLKQVDPDIVRVFILPPSMTELERRLRARGTDSEDVIRRRMERAAA
                     EISHWAEYDYVLINNDAEKCRELVHNILKAERLKATRRVFLHDFVRQLIG"
     gene            1072..1674
                     /locus_tag="VF547_12215"
     CDS             1072..1674
                     /locus_tag="VF547_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263630.1"
                     /translation="MIRTVIGGLVGGIILFVMGFIFWATPLSELAFSKAGDAEGAAVQ
                     AALAQNLTKTGTGTYEIPYAGTPQGTTLYGKGPIATVHFNTSGFSSEDMGMLLPGFIV
                     AVVTGLLMAFGLAAVGGGGRSFAGVARLVVLYSLGFTTWAYLAMPIFAHHGWGHYIYS
                     FIAESVSLILAGLVIARWFLPAAHSVPYREAAAAETREAA"
     gene            complement(1820..2509)
                     /locus_tag="VF547_12220"
     CDS             complement(1820..2509)
                     /locus_tag="VF547_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEX8263631.1"
                     /translation="MKRFYAHAAASPEEGGVAVLLDDRPVRTPGRNLLRVPTEELAEA
                     IAEEWNAQGETVDPQTMPLTGLANAAIDRVVPDPAGFAATLAQYGESDLLCYRADGPR
                     SLVERQERVWEPLLDWARTRFGVALETTTGVMHRAQPAETVERLGRAVAARGPFQLAG
                     LSPLVTIAGSLLIALALAEGAIRLQAAWEAATLDEAWQAEQWGEDALAAAALANRRRE
                     FEAAYRFLTLL"
     gene            complement(2506..2739)
                     /locus_tag="VF547_12225"
     CDS             complement(2506..2739)
                     /locus_tag="VF547_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263632.1"
                     /translation="MTPPDDETLWRNRFIAINLVRIGGTVIVLIGLAIWYSDLLFEGG
                     SMAVGLPLALAGLVASFWGPKVLAARWRTPPGP"
     gene            complement(2736..>3285)
                     /locus_tag="VF547_12230"
     CDS             complement(2736..>3285)
                     /locus_tag="VF547_12230"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX8263633.1"
                     /translation="VRELGFGIVFVATAEGLRAVHVPALLDGESLRFHVSRGNLAHSA
                     LAGGGDALFVATGPHAYISPEWYGLEDRVPTWNYVAVELEGPVRTLDPEELIRLVDDL
                     SAEQEARLAPKAPWTRGKMSPGRFEGLLKAISGFEMRVAQWRGTAKIDQDKPDSVRAR
                     IADALAGRGDPAMAAMMRKAAP"
BASE COUNT          495 a         1162 c         1126 g          502 t
ORIGIN      
        1 cggatcgggc ggcgccttcg acaccaccat ctgcagccgg ttcggggcgt cccgggccag
       61 ctccggaaac ggattggcct tcacataggc cgcccattgc ccggcggtcc ggacgatcgc
      121 cggcacatcg aggccgaagc ggtcgccgat cgccttttcg agctccgcct cgagccgatg
      181 ggcttcgccc ggcgcctcga acagcagatt gccgctctgg atgtaggtct cgacccgctt
      241 ccacccgagc tcgccgcaaa gggcgcgcag ctccgccatc ggcagcttgc gaccgccgac
      301 gttgaccgcg cgcaggaggg cgacgagccg ggccatgccc ctacctgctt gacgcatgcg
      361 ggctcggcaa gccagtagcg cgcatggaaa tcgcaagcgc catccagcgg cggggccttc
      421 tcttcatcct ctcgagcccg agcggcgccg gcaagtcgac catcgcccga atgctgctcg
      481 cgagcgacga cggcgtcgcc atgtcggtct cggcgacgac gcggccgaag cggtccggcg
      541 aggtggacgg gcgcgactat catttcgtcg acgacgccgg cttcgattcg ctgatcgccg
      601 gcggccattt cctcgaatgg gcgcacgtct tcggccatcg ctacgggacg ctcaagagcg
      661 aggtcctcaa gacgatcgag ggcgggcgcg acgtcctcct cgacatcgac tggcaaggca
      721 cccagcaatt gaagcaggtc gatcccgaca tcgtccgggt gttcatcctg ccgccgtcca
      781 tgaccgagct ggagcgccgg ctgcgcgccc gcggcaccga cagcgaggac gtcatccggc
      841 gccgcatgga gcgggcggcg gccgagatca gccactgggc cgaatatgat tacgtgctga
      901 tcaacaacga cgccgagaag tgccgcgagc tggtccacaa catcctcaag gcggagcggc
      961 tgaaggcgac ccgccgggtc ttcctccacg acttcgtgag gcagctgatc ggctgacttt
     1021 gcgccgggcc gctttgggcg taggctcctc ccgcaaccaa agggggtgac tatgatacgc
     1081 accgtgatcg gcggcctcgt gggcggcatc atcctgttcg tcatggggtt catcttctgg
     1141 gcgactccgc tgagcgagct ggccttttcc aaggcgggcg acgccgaggg cgcggcggtg
     1201 caggccgcgc tggcgcagaa tctgaccaag accggcaccg ggacctacga gatcccctat
     1261 gccggcactc cgcagggcac gaccctctac ggcaagggcc cgatcgcgac cgtgcatttc
     1321 aacacatccg gcttctccag cgaggatatg ggcatgctgc tgccgggctt catcgtcgcg
     1381 gtggtgacag ggctgctgat ggcgttcggc ctggccgccg tcggcggcgg cgggcgaagc
     1441 ttcgccgggg tcgccaggct ggtggtgctc tactcgctcg gcttcaccac ctgggcctat
     1501 ctggcgatgc cgatcttcgc ccatcacggc tggggccact atatctactc gttcatcgcg
     1561 gagagcgtgt cgctgatcct cgccgggctg gtcatcgccc gctggttcct gccggccgcc
     1621 cattccgtgc cctatcgcga ggccgcggcc gcggagactc gagaggcggc ctaggacaca
     1681 ggttccgccc aatccgctca tcctgagtag ccggtgagct tgtcgaaccg gcgtatcgaa
     1741 ggacgatcct tcgatacggg ccttcgcctt cgctcagtcc ctactcagga tgagcgattc
     1801 ggtgaaagac ggtcacggcc tagagcaagg tcaggaaccg gtaggcggcc tcaaactcgc
     1861 gccgccggtt ggcgagggct gcggcggcga gcgcgtcctc gccccattgc tcggcctgcc
     1921 aggcctcgtc gagcgtcgcc gcctcccagg cggcctggag ccggatcgcg ccctcggcga
     1981 gggcgagcgc gatcagcagc gacccggcga tggtcaccag cggcgacagg ccggcgagct
     2041 ggaaagggcc ccgcgccgcc accgcccggc cgagccgttc gaccgtctcg gccggctggg
     2101 cgcggtgcat gacgccggtg gtcgtctcga gcgcgactcc gaagcgggtc cgcgcccagt
     2161 ccagaagcgg ctcccagacc cgctcctgcc gctcgaccag cgagcgcggg ccgtcggcgc
     2221 ggtagcagag caggtcgctc tcgccatatt gggccagggt ggcggcgaag cccgccggat
     2281 cgggcacgac ccggtcgatc gcggcgttgg cgaggccggt cagaggcatg gtctgcgggt
     2341 cgacggtctc gccctgcgcg ttccactcct cggcgatcgc ctcggcgagc tcctcggtcg
     2401 ggactcgaag caggttgcgg ccgggcgtgc ggaccggccg gtcgtcgagc aggacggcga
     2461 ccccgccctc ctcgggcgag gcggccgcgt gagcgtagaa gcgcttcatg gccctggcgg
     2521 ggtcctccac cgcgccgcca ggaccttcgg gccccagaag ctggcgacca ggccggccag
     2581 cgccagcggc agcccgaccg ccatcgaccc gccctcgaag agcaggtcgc tgtaccagat
     2641 ggcgaggccg ataaggacga tgacagtgcc gccgatccgc accaggttga tcgcgatgaa
     2701 gcgattgcgc cagagggtct cgtcgtccgg gggggtcatg gggccgcctt cctcatcatc
     2761 gccgccatcg ccgggtcgcc gcgtccggcg agcgcgtcgg cgatcctcgc ccgtaccgag
     2821 tcgggcttgt cctggtctat cttggcggtg ccccgccact gcgcaacccg catctcgaac
     2881 cccgagatcg ccttgagcag cccctcgaac cggcccgggc tcatcttgcc gcgggtccag
     2941 ggcgccttcg gtgcgagccg cgcctcctgc tcggcggaaa gatcgtcgac gaggcgaatc
     3001 agctcctccg gatcgagcgt ccgcaccggc ccttcgagct cgacggcgac atagttccag
     3061 gtcggcaccc ggtcttccag cccgtaccat tccgggctga tataagcatg cgggccggtg
     3121 gcgacgaaca gggcgtcgcc gcccccggcc agcgccgagt gagcgagatt gccgcgcgag
     3181 acgtggaagc gcaggctctc gccgtcgagc agcgccggca cgtggacggc ccggagccct
     3241 tcggcggtgg cgacgaagac gatcccgaag cccagctcgc gaacc
//
LOCUS       DASVQZ010000434         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_667269, whole genome shotgun sequence.
ACCESSION   DASVQZ010000434 DASVQZ010000000
VERSION     DASVQZ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667269"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VF547_12235"
     CDS             complement(<1..1356)
                     /locus_tag="VF547_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX8263634.1"
                     /translation="MTRFVYRFGGGVADGEAGNRNLLGGKGANLAEMASIGLPVPPGF
                     TISTEMCARYYAEGEGFPDSLRAEVAEGLAHIEGICGKRFGDPADPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDSTVEGLASVSGDPRFAWDSYRRFIQMYSNVVLGLKHDLFEEAL
                     EIAKEDKGVHLDTELEAGDWRRLTARYREIVGEELGRPFPEDVGEQLWGAIGAVFGSW
                     QSDRAKTYRRLNAIPGDWGTAVNVQAMVFGNMGETSATGVAFTRNPSTGERAFYGEYL
                     INAQGEDVVAGIRTPQYLTRAAREAAGARAPSMEESMPEVYAELTRVFDRLERHYRDM
                     QDIEFTVERGKLFMLQTRSGKRTAKAALRIAVDMAGEGLVTQEEAVLRVDPLALDQLL
                     HPTLDPAAPRNVIAKGLPASPGAASGAAVFDADTAEKRAALGEKVVLVRVETSPEDIH
                     GM"
     gene            complement(1463..2632)
                     /locus_tag="VF547_12240"
                     /pseudo
     CDS             complement(1463..2632)
                     /locus_tag="VF547_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
BASE COUNT          407 a          962 c         1000 g          447 t
ORIGIN      
        1 catgccgtga atgtcttcgg gcgaggtttc gacgcggacg aggacgacct tttcgcccag
       61 ggccgcgcgc ttttcggccg tgtccgcgtc gaacaccgcg gcgcccgagg cggccccggg
      121 cgaggccgga aggcccttgg cgatgacgtt gcgcggcgcg gcgggatcga gcgtcgggtg
      181 gagcagctgg tcgagcgcca gcggatcgac ccggagcacc gcctcctcct gcgtgaccag
      241 cccctcgccg gccatgtcga ccgcgatccg aagcgccgcc ttggcggtgc gcttgcccga
      301 gcgggtctgg agcatgaaca gcttgccgcg ctcgacggtg aactcgatgt cctgcatgtc
      361 ccggtagtgg cgctcgagcc ggtcgaagac ccgggtcagc tcggcataga cctcgggcat
      421 cgattcttcc atcgacggcg cccgggcgcc ggccgcctcg cgcgccgccc gggtcagata
      481 ttgcggggtg cggatgccgg cgacgacgtc ctcgccctgg gcgttgatca gatattcgcc
      541 gtagaaggcg cgctcgccgg tcgaggggtt gcgggtgaag gcgaccccgg tggccgaggt
      601 ctcgcccatg ttgccgaaca ccatcgcctg gacgttgacc gccgtgcccc agtcgcccgg
      661 gatggcgttg aggcgccggt aggtcttggc ccggtccgac tgccaggagc cgaacacggc
      721 gccgatcgcg ccccagagct gctcgccgac gtcctcgggg aagggccggc ccagctcctc
      781 gccgacgatc tcgcggtagc gggcggtgag ccggcgccag tcgccggcct cgagctcggt
      841 gtcgagatgg acgcccttgt cctccttggc gatctcgagc gcctcctcga acaggtcgtg
      901 cttcaggccg aggacgacgt tcgagtacat ctggatgaag cggcggtagc tgtcccaggc
      961 gaagcgcggg tcgcccgaga cggaggcgag gccctcgacc gtggaatcgt tgaggccgag
     1021 gttgaggacg gtgtccatca tgcccggcat cgacacccgg gcgccggagc ggaccgagac
     1081 cagcagggga tcggccgggt cgccgaacct cttgccgcag atgccctcga tatgggcgag
     1141 gccttcggcg acctcggcgc gcaggctgtc cgggaagccc tccccttccg catagtagcg
     1201 cgcgcacatc tcggtcgaga tggtgaagcc gggcggcact gggaggccga tcgaggccat
     1261 ctcggcgagg ttggcgccct tgccgccgag caggttgcgg ttgcccgcct cgccgtcggc
     1321 gaccccgccc ccgaagcggt agacgaagcg ggtcacgtcc gttcctcgtc atcccggcgg
     1381 aggccgggac cgcggggaaa ccggccgcac cttcccgtac cggggccgcg gcttgcgccg
     1441 ggaggacgga ctgccacccc cctcacccct cgatccgcga gaagtcggcg acccggtgga
     1501 cggcgtcgcg catccgcgcg aggagcgcga gccgggcttc gcgcctggcc gggtcgggat
     1561 cgttgacggt caccttgtcg aagaacgaat cgaccgggcc gcgcaggctg gccagcgccg
     1621 ccatcgcccc ctcgaaatcc tccgcctcga cggcggcgcg cgcccgcggc tcggccgaat
     1681 cgagggcggc gatcagcgcc gcttcctccg gttcgggctc gtagggaaga ggcttgttct
     1741 gtgtgcgttc cgtcatgccg gcggaggctg gcatccatcc ggagtgggtg cctgcgccgg
     1801 actcggtatg gaccccagcc ttcgctgggg tgacgtagcc ctccttcttg aggatgttcg
     1861 ccgcgcgctt gtagccggcg aggaggtcgg cgccctcggg cgtctcgacg aaggcctgga
     1921 gcgccttcac ccgcgcgagc agccggacga gatcgtcctc gccgccgagc gcgaagacgg
     1981 cgtcgatcag gtcgtggcgg acgcctgcct cgcgctgctg gaccttgagg cggtcaacga
     2041 cgaatgtatt cagctcttcg ggcgcctccg aaatccccag acggacggct cggcccatat
     2101 cgttgaaggc cgttatcgag ctttcggtaa tgccgtattc gtcctgatcc gggcggttgg
     2161 gatactcaaa ctcggcgagc acatgagcta ctgccgcgcc cacaattggc gtgagcatcg
     2221 ctctcatcgt cgacgaatag gtcgcttcag ctgccgcttg gaaagctcgc gtccacgaaa
     2281 gtcgcaggtc gttcgaaact acgatcgaga caacaccaag catcgctctt cgaagtgcga
     2341 atggatcctt cgacccggtg ggcaattcgt tgatgacaaa gaaggcggcc aagctgtcca
     2401 gcttgtcggc caggctcacc gccaccgtga ccggcgcggt cggcacctcg tcgccctgcc
     2461 ccaccggctt gtaatggtcg cggatcgcgt cggcgacggc ggggtcctcg ccctgggccc
     2521 tcgcgaggtg gccgccgatg acgccctgga gctcggggaa ctcgccgacc aggccggtga
     2581 cgaggtcggc cttgcagagg cgggcggcgc gttcggcgag ggcggcgagc tgctccctct
     2641 ccccttcagg ggagagggct ggggagaggg ggcggccggt cggcgtcgcg gaggtcgggg
     2701 cttggctgga ttgccccccg cccactttgc aatcgcccga ccaggcctgc catcgcacga
     2761 gagcctccaa aaatccgccg cgcccgccgt ggcatgggct accagcccat gattca
//
LOCUS       DASVQZ010000435         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_860760, whole genome shotgun sequence.
ACCESSION   DASVQZ010000435 DASVQZ010000000
VERSION     DASVQZ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860760"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..622)
                     /locus_tag="VF547_12245"
     CDS             complement(152..622)
                     /locus_tag="VF547_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263635.1"
                     /translation="MTQIDDLAISKLFSPLAGWAEHRLGFGQWRIALECLNGHVAFYL
                     AGLAFSLTGKGMEDGIFASLLVALAWLLLMEAVRRVARRQAGSSLGVQTARLREWHFR
                     LILLVSLPISVARIDEVANACYSVSLLLLLCHLYFKACDSPPPQGRRRLAYNRG"
     gene            762..974
                     /locus_tag="VF547_12250"
     CDS             762..974
                     /locus_tag="VF547_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263636.1"
                     /translation="MKAFPVAALLLLAGCQDSAADNNAAALERAAEQSSPEAADVLRN
                     AAENGAEVQDAMQAAGNAQAAADNRQ"
     gene            complement(979..1485)
                     /locus_tag="VF547_12255"
     CDS             complement(979..1485)
                     /locus_tag="VF547_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HEX8263637.1"
                     /translation="MNAVTAFLGDSIVARGERAAVTRELEDRYPADHGAIRVFEDGTG
                     RLVDLDYWDAAASAPAPPRRRGRPSLGVAAREVTLLPRHWEWLSRQPGGASAAIRRLV
                     EDARRKGGGPGEKRDSAYRFLSHMCGDRPGYEEALRALYRGEEERFRTLIEPWPPAIR
                     GYVEALLA"
     gene            1657..2715
                     /locus_tag="VF547_12260"
     CDS             1657..2715
                     /locus_tag="VF547_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX8263638.1"
                     /translation="MAQQAATAPLPPAPGPAEIAANPLAQRSLRSPRHTYGALDLGTN
                     NCRLLIARPAEEGFVVIDAFSRVVRLGEGLASSGRIADAAMDRAVAALSVCAEKLKRR
                     HVSLARSVATEACRRAVNGRHFVDRVRRETGICLEIISPEEEARLAMLGCHRLLEPGD
                     APAQIFDIGGGSTELILIDTDGEGTPKIKCWWSAPWGVVSLTESEGRDFADAAERLAA
                     YGRMKERVRHAFRRFAELLPPREGNIRLLGTSGTVTTLASVHLALPSYDRRAVDGLMM
                     PADSMRAVNRMLSGMSFSERAELPCIGAERADLVVAGCAILEAIMDIWPARTLGVADR
                     GIREGILRSLMAKDGHRL"
     gene            2855..3511
                     /locus_tag="VF547_12265"
     CDS             2855..3511
                     /locus_tag="VF547_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX8263639.1"
                     /translation="MVTRVRTAKGRKVGSTKWLERQLNDPYVKRAKAEGYRSRAAYKL
                     VELDDRFGLLKGAERVVDLGVAPGGWTQVVRRRAPKAVVVGIDLLPTEPIDGARLLQL
                     DFLAEDAPERLREALGGPADLVLSDMAANTVGHPQTDHLRTMALVEAGLEFAGEVLRP
                     GGAFVAKVLAGGADNHLVAELKRRFSSVKHAKPPASRKGSSEWYVVAQGFKGGLAETE
                     "
     gene            3576..3941
                     /locus_tag="VF547_12270"
     CDS             3576..3941
                     /locus_tag="VF547_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX8263640.1"
                     /translation="MTVDTARQAAELLAPHFAAREGEAVAALHLDAERRLLGTTVADA
                     DPATLPVRDILAAALRLGAVALIIARHRPGGDPMPTEEDRAAARRLAEAGASLGVRLT
                     DHFIFAGDECHSFRELGLL"
BASE COUNT          640 a         1458 c         1466 g          582 t
ORIGIN      
        1 gggcgctggt cgaagccgcc tacgagcggc tccgggcgaa gggctgcggg ctcgtcgaag
       61 tcaccagcaa cgtcgatctg tcgggcgcgc attccttcta ccgaaagctc ggcttcgagc
      121 ggacgagcta ccggttcgcg aagaccttgg actagcctcg attgtaggcg aggcgtcggc
      181 ggccctgggg cggcggggag tcgcacgcct tgaagtagag atggcacagg agcagcagaa
      241 gcgacacgct gtagcaggcg ttggcgacct catcgatgcg cgcgacgctg atcggcagcg
      301 acaccagcag gatcagccgg aaatgccact cgcgcagccg cgccgtctgc acgccgagcg
      361 aggatccggc ctggcggcgg gcgactcgcc ggaccgcttc catcagcagc agccaggcca
      421 gcgcgaccag caggctggcg aaaatcccgt cctccatgcc cttcccggtg aggctgaagg
      481 ccagccccgc caggtagaag gcgacgtgcc cgttgaggca ttcgagcgcg atccgccact
      541 ggccgaagcc gaggcggtgc tcggcccagc ccgccagcgg cgagaacagc ttggaaatgg
      601 cgagatcgtc gatttgggtc acggctccgt ttatcaagcc aggaagttga gaaatcttaa
      661 aaaacgggag catggttaat tttcccgcgg ctgttgcatc gccgcaaccc ccgggccctc
      721 gccctcgttg tcccaggacc atccgttcga ggagatcccc gatgaaggca ttcccagtgg
      781 cggcgctcct cctgctggcc ggctgccagg acagcgcggc cgacaacaat gccgccgcgc
      841 tcgagcgtgc cgccgagcag agctcgcccg aagccgcgga cgtgctgcgc aacgccgccg
      901 agaacggtgc cgaggtccag gacgcgatgc aggccgccgg caatgcccag gcggcggcgg
      961 acaaccgcca gtagccgatc aggccagcag ggcctcgaca tagccgcgga tcgccggcgg
     1021 ccagggctcg atcagcgtcc ggaagcgttc ctcctcgccg cgatagaggg cccgaagcgc
     1081 ctcctcgtag ccgggccggt cgccgcacat gtgcgacagg aagcggtagg cggaatcgcg
     1141 cttctctccg gggcccccgc ccttgcggcg cgcgtcctcg accagccgcc ggatcgccgc
     1201 ggaggcgcct ccgggctggc gcgacagcca ttcccagtgg cgaggcaaga gggtcacctc
     1261 ccgcgccgcg actcccaggc tgggccggcc gcgccgccgc ggcggcgccg gcgccgaagc
     1321 cgccgcgtcc caataatcga ggtcgacgag ccggccggtg ccgtcctcga agacgcggat
     1381 cgcgccgtgg tcggccgggt agcgatcctc gagctcgcga gtcactgccg cgcgctcgcc
     1441 ccgcgccacg atcgagtcgc ccagaaaggc cgtcacggca ttcattctcc agctcccttc
     1501 ccaatataaa tatccggata aaactaccgg gtcaatatcg tccgggtata atcccgcgtc
     1561 gggccggtgc ccttgccgat gcgggccgtg ggcggtacga ggggagcaag tcaccgtcgg
     1621 gtccgcccgg cggaacgaga aaagagtgtc accgctatgg cgcagcaggc tgcgacggcc
     1681 ccgctcccgc cggctcccgg tccggcagaa atcgccgcga atccgctcgc gcagcggagc
     1741 ctgcgttctc cccgccacac ctacggcgcg ctcgatctgg gcaccaacaa ctgccggctg
     1801 ctgatcgcgc gtccggccga ggagggattc gtcgtcatcg acgccttctc gcgggtggtg
     1861 cggctgggcg agggcctggc gagcagcggc cgaatcgccg acgcggcgat ggaccgggcg
     1921 gtggccgcgc tctccgtctg cgccgagaag ctgaagcgcc ggcacgtctc gctggctcgc
     1981 tcggtcgcga ccgaggcctg ccggcgagcc gtcaacggcc gtcatttcgt cgaccgggta
     2041 aggcgggaaa ccggcatctg cctggagatc atctcgcccg aggaggaggc gcggctcgcc
     2101 atgctcggct gccaccgcct gctcgagccc ggcgacgccc cggcccagat cttcgacatc
     2161 ggcggcggct cgaccgagct catcctgatc gacaccgacg gcgagggcac gcccaagatc
     2221 aaatgctggt ggagcgcccc ctggggcgtg gtctcgctga ccgagagcga gggccgcgac
     2281 ttcgccgacg ccgccgagcg gctcgccgcc tacggccgga tgaaggagcg ggtgcgccac
     2341 gccttccgcc gcttcgccga gctgctgccg ccgcgcgagg gcaacatccg gctgctcggc
     2401 accagcggca ccgtgacgac cttggccagc gtccacctcg cccttcccag ctacgaccgg
     2461 cgggcggtcg acgggctgat gatgcccgcc gattcgatgc gcgccgtcaa ccggatgctg
     2521 tccgggatga gcttctcgga gcgcgccgag ctcccctgca tcggcgccga gcgggccgac
     2581 ctcgtcgtcg ccggctgcgc gatcctcgag gccatcatgg acatctggcc cgcccgcacc
     2641 ctcggcgtcg ccgaccgcgg catccgcgag gggatattgc gctcgctgat ggccaaggac
     2701 gggcacaggc tgtgatgatc ctccccatcg aagatgggga gggggaccag gcgaagcctg
     2761 gtggaggggc ggatgacggg gcttccgccg cttacccctc caccaccctt cgggtggtcc
     2821 ccctcctcga gcaagctcgg ggaggacttc gccgatggtg acccgggttc gcaccgccaa
     2881 gggccgcaag gtcggctcca cgaagtggct cgagcggcag ctgaacgatc cctatgtcaa
     2941 gcgcgccaag gcggagggct atcgctcgcg cgccgcctac aagctggtcg agctcgacga
     3001 ccgcttcggt ctgctgaagg gcgccgagcg ggtggtggac ctcggcgtcg cgcccggcgg
     3061 gtggacccag gtggtgcgca ggcgcgctcc gaaggcggtg gtggtcggca tcgacctcct
     3121 gccgaccgag ccgatcgacg gcgcccggct cctccagctc gatttcctcg ccgaggacgc
     3181 tcccgagcgg ctgcgcgagg cgctgggcgg gccggccgac ctcgtcctct ccgacatggc
     3241 tgcgaatacc gtcggccatc cccagaccga ccatctgagg accatggcgc tggtcgaggc
     3301 ggggctcgaa ttcgccggcg aagtgctgag gccgggcggc gcgttcgtcg ccaaggtgct
     3361 cgcggggggc gcggacaatc atctggtcgc ggagctcaag cggcgctttt cgagcgtgaa
     3421 gcacgccaag ccgccggcca gccgcaaggg ctcctccgaa tggtatgtgg tggcgcaggg
     3481 cttcaagggc ggtttggcgg aaaccgaata aaatcgactc ggaattgaac atattcgctg
     3541 gagcctgagg cgggccgcct tggcaaggac cgggcatgac cgtggacacg gcgcgccagg
     3601 cggccgagtt gctcgcgccc catttcgcag cccgcgaagg cgaggcggtg gccgcgctcc
     3661 acctcgacgc cgaacggcgc ctgctcggca ccaccgtcgc cgacgcggat ccggccacgc
     3721 ttccggtccg cgacatcctc gccgcggcgc tgaggctcgg ggccgtggcg ctgatcatcg
     3781 cccgccaccg gcccggcggc gacccgatgc cgacggagga ggaccgcgcc gccgcccgcc
     3841 gcctcgccga agccggcgcc tcgctgggcg tgcggctgac cgatcatttc atcttcgccg
     3901 gcgacgagtg ccacagcttc cgcgagctgg gcctgctcta gctgtgagct ttccataggt
     3961 gttcatccgg ccctggccga gcagactgag gttgcgctcg ctaccgggct gagggtgagg
     4021 ccggccggcg ctcgtcgggc cgggcgggga agggcggtca gccggccacc gaggcggggc
     4081 agacggggat gtccttgggc tcgggccggg gcccgctcgg ctcgaactgg gcgaaatagg
     4141 attcgg
//
LOCUS       DASVQZ010000436        12202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_89597, whole genome shotgun sequence.
ACCESSION   DASVQZ010000436 DASVQZ010000000
VERSION     DASVQZ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89597"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF547_12275"
     CDS             complement(<1..624)
                     /locus_tag="VF547_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8263641.1"
                     /translation="MEVGIFVPIGNNGWLISENSPQYMPSFDLNKAIAQKAESYGVDF
                     LLSMIKLRGFGGKTEFWEYNLESFTLMAGLAAVTEKIKIYATCPTLVIPPAFCARMCN
                     TIDSISHGRFGLNLITGWQRPEYSQMGLWPGDEHFRNRYQMLGEYAHILRELWATGVS
                     DFKGEYYRMEDCRVRPLPEGEMKIICAGSSDEGLAFSAQYADYAFCLG"
     gene            complement(629..1393)
                     /gene="rutD"
                     /locus_tag="VF547_12280"
     CDS             complement(629..1393)
                     /gene="rutD"
                     /locus_tag="VF547_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HEX8263642.1"
                     /translation="MPNAAGLWYEWHGPEDGEVLILSPGLGGSGSYWAPNLAALSKDH
                     RVLLYDHRGTGRSGSLPEGEVSIRSMAADVVALMDELGIRSPYFLGHAVGGLIGLELG
                     SVLAKRVVVNGWPRLDPHTARCFEVRLELLRASGPQAYVQAQPIFLYPAGWSSMNSGM
                     LDLEAEHQLERFPPVATVERRIAAARAFELRPGPACPTLLVAAADDLLVPAHCSEFLA
                     GELANARVAPLGWGGHACNVTDPDAFNALVLDFLRS"
     gene            complement(1393..1776)
                     /gene="rutC"
                     /locus_tag="VF547_12285"
     CDS             complement(1393..1776)
                     /gene="rutC"
                     /locus_tag="VF547_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HEX8263643.1"
                     /translation="MPFESINPPQFPTPIAPYSAGTRAGGTVYVSGTLALGPGGTVLY
                     PGDAEAQTRHVLETIRITLEAAGASLADVAFNHIFLTDWAHYPAMNAVYAEYFPGDKP
                     ARYCIQCGLVKPGLVVEIASVAHIG"
     gene            complement(1876..2595)
                     /gene="rutB"
                     /locus_tag="VF547_12290"
     CDS             complement(1876..2595)
                     /gene="rutB"
                     /locus_tag="VF547_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein B"
                     /protein_id="HEX8263644.1"
                     /translation="MTVDATSDGKTRRLVTLPARPEPIRVAAEETAVIVVDMQNAYAS
                     KGGYLDLAGFDIGGAAKVVEQTAKVVETARAAGLPIIYFQNGWDAAYVEAGGPGSPNW
                     HKSNALKTMRARPELQGTLLARGGWDYALVETLAPHPGDIVLPKTRYSGFFNSQLDSV
                     LRSRGIRNIVLVGIATNVCVESTLRDGFFLEYFGIMLEDATHQAGPEYLQKASVYNVE
                     TFFGWVSTVADFCGAFGQIGE"
     gene            complement(2717..3304)
                     /locus_tag="VF547_12295"
     CDS             complement(2717..3304)
                     /locus_tag="VF547_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX8263645.1"
                     /translation="MPRQPDHPVTPLFLERWSPRAFDGSQIPEEDLLTMFEAARWAPS
                     AFNSQPWRFLYARRGGADWERFLDLLIPWNRSWAQGASALVYILSDTLPFTDKQTGDP
                     RPSTTHGFDAGAAWACLALQATRMGYHAHGMSGIQYELARSELRIPERFVLNAACAIG
                     RIGDPAQLDEKLRAREFPSGRKPVQEFAFRGGWPQ"
     gene            3566..8227
                     /locus_tag="VF547_12300"
     CDS             3566..8227
                     /locus_tag="VF547_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX8263646.1"
                     /translation="MTSDSLVADAPAADVQIVDALAHALVDGALPGELADFTDGDRIA
                     AAQFIAACAARRPPGIALVRVEPQGGALGHRRMRIGIVNDDMPFLVDSVANTLAARNL
                     TVHRLLHPVVCVTRDEQGCLQAVEPLCDDAERRESMMYLEVDRDDARGRREIVVDLQR
                     VLADVRAAVTDWRAMQARMRADADQAANEEGAALLRWFADGAMTLLGYEVERPDGPPT
                     DALGLFRIPGEPTDPGGALGAMRYFEQGGDVPLLAKAERRASVHRRVPLDLVVVPVRE
                     GGKVTGIGVHAGLWTSEALSSPVEEVPLLRKRLQQLEREFGFDPSGHSGKALRHAVTA
                     LPHDLLVSLSVEAVRELVTTAMSLADRPRPTLSLVRSILKGNMFAFVWLPRDELTTRR
                     RLAIGAMIEQASQGRISNWSVELGDGDLALIRYTLYVGPETPTPDVAELDRRLDEMVR
                     GWEPSVEEALGDLVGPQRATRLAITYLDDFPEGYRLTVPPPEAAQDILRICALGGASQ
                     RDARLYRQAEDGPRRLRLNTYRLAEIIPLSDAVPVFENFGFRVLEEHPTALDEGRLGY
                     VHEFLLEVRSGEAAQVLDRAGLVERAIAEVLEGRAENDPFNQLLVAAALAPRDVVLFR
                     AWFRYLRQGGLSYSMVTVVEALRRAPGVAHGLIALFDALHDPSHEEPRDAAAAAAERA
                     IRDGLSQVAAIDEDRILRLLHAVVSATLRTNAFAPAGREALAFKLDSARVPNLPQPLP
                     WREIWVYSPRVEGIHLRGGPIARGGLRWSDRRDDFRTEILGLMKAQLVKNAVIVPTGA
                     KGGFYPKQLPPIAERDAWLAEGTESYRIFIRALLSITDNIVGNKVVHPDGIVVHDGQD
                     PYFVVAADKGTATFSDVANAIATERGFWLGDAFASGGSYGYDHKAMGITARGAWISVQ
                     RHFRELGIDVQTQPIRVAGVGDRSGDVFGNGMLLSKSIKLVAAFDHRHIFLDPDPDPA
                     ASWEERNRLFGLPRSSWDDYDRKLISKGGGIFPRDQKAIPLSPEVRALLGVEGEETDP
                     ATLLTAILSSPVDLLWFGGIGTYIKAKSESNAEVGDPANDAIRVNGAELRAKAIGEGA
                     NLAITQAGRVEFAGLGGRINTDFIDNSAGVDCSDNEVNIKIPLNREMAEGRLGLEDRN
                     ALLAEMTDEVAAIVLEDNRLQTLALSVAERSGATGLPPLLRAMEMLEESGRLNRAVEG
                     LESNEELMRRGQENRGLTRPELAILLSSSKMALQAALEAGKITEDPTLTPELCNAFPE
                     TMRNRHGDAILAHRLRREIIATKIANRLVNRLGITAPFALAEEEGASLGQVAAAFVAA
                     ERLFDMQGFWERLDTAALPEQVRLELFDQASRALQLHMADILRNMSASAKLAEIVDNL
                     KPGLDSLDEAVEGLLRREVASEASGRRQRLIELGAPEDIAHKIVRLFELNGGVGIAAL
                     GKRLGVDVIELTNAYTRLGEALGLDWAQGAANRFQAKDQWERLLTAGLARDFEQLRLD
                     FLHRKRGTDPVVAVDQWVLAQGPRVEQFRRVVERVRNAPVTTAPMLAQIATQARVLLG
                     R"
     gene            8268..10544
                     /locus_tag="VF547_12305"
     CDS             8268..10544
                     /locus_tag="VF547_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8263647.1"
                     /translation="MDPTFAGVTRRISRSISTAMARGGERRGISRRTLLVGGGAGAGL
                     VLAWALWPRTLEPNLRAGEGEHVFNAFLKIGTDGRIVVAVPQAELGQGSWTALPQILA
                     DELGADWRTVGVEPAPLGPVYANRLLGEGEDDGSFAGGVGRWRARNFEGGEAAMLTAG
                     STSVRAFERPLREAGAGARALLMKAAASRWGVDWETLDTAAGFVLEGDRRLAFAELAE
                     AAAEETLPAYLPIRGGIDNRLAGQPLPRLDLPSKVDGSAPFAGDVRLPEMVHASVRAG
                     PTEDSRLAGWDRTAALAVSGVLGVFDAPGWVACVANNWWAADQGVEALRARWHVPRPP
                     LTAESIDSALVEALERDEGSRVFSRGDLADAFEGGNVLRGHYRAGPAPSAPLETLTAT
                     ARLTGGRLEIWAPAQAPGLARAAAARVAGLGEAEVTVYPTLIGGGYGRKLETRAIEQA
                     AMLAMRTRRPVQLAWSRLEESVQDGFRAPARAQLLARLDEGGVILGWQARIAAPDTGA
                     ELEARLRGGRTAMAAIGDPTAGAVPPYAIPAVAVDYLPAETGLRIGAWRSGAHSYCAF
                     FTESFMDELARISGIEPLSFRMQMLGDNPRLAQCLTTAATLGGWDGGTPGSAMGIACH
                     SAFGSHIATLVEIEVTAEQRIRVLRAICAVDCGRIVNPEIVRQQVEGGLVFGIAAATG
                     QEIGFEDGRPDLRGLGQFGFPTLAGSPDVTVELLDSEEEPGGVTELAVPTAAPAVANA
                     LHALTGRRLRTLPLRVGG"
     gene            10541..11551
                     /gene="hemH"
                     /locus_tag="VF547_12310"
     CDS             10541..11551
                     /gene="hemH"
                     /locus_tag="VF547_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX8263648.1"
                     /translation="MTARPADHPKVPPPRIGVLLVNLGTPDKPEPGSVRLYLKEFLSD
                     PRVIEIPQLVWQPILRGIVLTTRPRKSAHAYRQVWTGEGSPLAAITARQAKALAGSWG
                     ENVVVDHAMRYGKPAIAARIDALRAQGCERILIAPLYPQYCAATTATANDEAFLHLRR
                     LRWQPAVRTLPPYYDDPAYIAALAERLAQALAGLDFEPQAILASFHGMPQRTLELGDP
                     YHCHCRKTARLLEEASGRELRVVFQSRFGRAKWLQPATDETLAALPAEGVTRVALLAP
                     GFAADCLETLEELAIRGRDTFLAAGGSDFAYLPCLNDSPSGLQMLRSILGRELEGWRD
                     AP"
     gene            11588..>12202
                     /locus_tag="VF547_12315"
     CDS             11588..>12202
                     /locus_tag="VF547_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8263649.1"
                     /translation="MARVAIVTGGTRGIGEAISVALRDQGVTVAANYAGNEEKAREFS
                     DRTGIKAYKWDVSDFDACLAGVAQVESELGPVDVLVNNAGITRDGTMKRMTRQAWDEA
                     IDVNLGGCFNMAKAVFEGMQSRKFGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGARGGVTVNAIAPGYIDTEMVAAVPEDVLQKIVARIPVGRL"
BASE COUNT         1841 a         4330 c         4276 g         1755 t
ORIGIN      
        1 gccgaggcag aaggcatagt ccgcatattg ggccgagaag gccaggccct cgtccgacga
       61 tccggcgcag atgatcttca tctcgccctc gggcagcggc cgcacccggc aatcctccat
      121 ccggtaatat tcgcccttga agtcggagac cccggtcgcc cacagctcgc gcaagatgtg
      181 ggcatattcg ccgagcatct ggtagcggtt gcggaaatgc tcgtcgccgg gccacagccc
      241 catctggctg tattcggggc gctgccagcc ggtgatcagg ttgagtccga agcggccgtg
      301 gctgatcgaa tcgatcgtgt tgcacatccg cgcgcagaag gcgggcggga tgacgagcgt
      361 cgggcaggtg gcgtagatct tgatcttctc ggtgacggcg gcgaggccgg ccatcagggt
      421 gaagctctcc agattatatt cccagaactc ggtcttgccg ccgaagccgc ggagcttgat
      481 catcgacagc aggaagtcga ctccgtagct ctccgccttc tgggcgatcg ccttgttgag
      541 atcgaagctc ggcatgtatt gcggcgagtt ctcggagatc agccagccgt tgttgccgat
      601 gggcacgaag atgccgactt ccatggtttc agctccttag aaaatcgagg acgagggcat
      661 tgaaggcgtc cggatcggtg acgttgcagg catggccgcc ccatccgaga ggcgcgaccc
      721 tggcgttggc gagctccccg gccaggaatt cggagcaatg cgcggggacc agcaggtcgt
      781 cggccgcggc gacgagcagg gtcgggcaag cggggccggg ccgaagctcg aacgcccgcg
      841 ccgccgcgat ccgcctctcg accgtcgcga ccggcgggaa gcgctcgagc tggtgctccg
      901 cctcgagatc gagcatcccg gagttcatcg agctccaccc ggccgggtag aggaagatcg
      961 gctgggcctg gacataggct tgcgggccgc tcgcccggag cagctccagc cggacctcga
     1021 agcaccgcgc cgtgtgcggg tcgagccgcg gccagccgtt gaccaccacc cgcttggcca
     1081 gaacgcttcc gagctcgagc ccgatcagcc cgccgacggc gtgaccgagg aaataggggc
     1141 ttcggatgcc gagctcgtcc atcagggcga cgacgtcggc ggccatcgag cggatcgaca
     1201 cctctccctc gggaagcgag ccgctccggc cggtcccgcg atgatcgtag agcaggaccc
     1261 ggtggtcctt cgacagcgcc gccaggttgg gagcccaata ggagcccgag ccgccgaggc
     1321 cgggcgagag gatcaggacc tcgccgtcct ccgggccgtg ccattcgtac cagaggcctg
     1381 cggcattcgg catcagccga tatgcgccac gctggcgatc tcgaccacca ggcccggctt
     1441 caccaggccg cactggatgc agtagcgcgc cggcttgtcg ccgggaaaat attcggcgta
     1501 gacggcgttc atcgccggat agtgcgccca gtcggtcagg aagatgtggt tgaaggcgac
     1561 gtcggcgagg ctcgccccgg ccgcctccag ggtgatccgg atcgtctcca gcacatgccg
     1621 ggtctgggcc tcggcgtcgc cggggtagag caccgtgccg cccggcccga gcgccagcgt
     1681 cccggagacg tagacggtgc cacccgccct cgtcccggcc gaataaggcg cgatcggggt
     1741 cgggaattgc ggcgggttga tcgattcgaa cggcatccaa gtctccttcg tcattgcgag
     1801 cgaagcgaag caatccagtc cgcggcggtg gaggcgcgta gattgccgcg tcgcttcgct
     1861 cctcgcaatg acggattatt ccccgatctg gccgaaggcg ccgcagaaat cggcgacggt
     1921 cgaaacccag ccgaagaagg tctcgacgtt gtagacgctc gctttctgga gatattcggg
     1981 gccggcctgg tgggtcgcgt cctcgagcat gatcccgaaa tattcgagga agaagccgtc
     2041 gcgcagagtc gattcgacgc agacgttggt ggcgatcccg accaggacga tgttgcggat
     2101 gccgcgcgag cgaagcaccg aatcgagctg cgagttgaag aagcccgaat agcgcgtctt
     2161 gggcaggacg atgtcgccgg gatggggggc gagggtctcg acgagggcgt agtcccagcc
     2221 gccgcgggcg agcagcgtgc cctgaagctc gggccgcgcc cgcatcgtct tgagcgcatt
     2281 ggacttgtgc cagttgggcg agcccggccc gcccgcctcg acataggccg catcccagcc
     2341 gttctggaaa tagatgattg gaaggccggc ggcgcgggcc gtctcgacca ccttcgccgt
     2401 ctgctcgacc accttcgccg ccccgccgat gtcgaagccg gcgaggtcga gatagccgcc
     2461 cttggaggca taggcgttct gcatgtcgac gacgatcacc gcggtctcct ccgcggcgac
     2521 ccggatcggc tccgggcggg ccggaagggt gacgaggcgc ctcgtcttgc cgtcgctggt
     2581 cgcgtccacc gtcataggcg ggaatgttga ccagaaggcg cgctcgcaca acccgtcatg
     2641 ccagcgcaag ctggcatcca tgcggaggcg tgcccgtacc gatgccgcat ggaccccagc
     2701 ctgcgctggg gtgacgctat tggggccacc cgccccggaa ggcgaattcc tgcaccggct
     2761 tgcgcccgct cgggaactcg cgcgcccgca gcttctcgtc gagctgggcg gggtcgccga
     2821 tccggccgat cgcgcaggcc gcgttcagca cgaaacgctc gggaattcga agctcggagc
     2881 gggcgagctc gtactggatc cccgacatgc cgtgggcgtg ataacccatt cgggtggcct
     2941 gcagcgccaa gcaggcccag gcggcgccgg catcgaagcc gtgggtggtc gaaggacgtg
     3001 ggtcgccggt ttgcttgtcg gtgaagggca gggtgtcgga gaggatgtag accagcgccg
     3061 aggcgccttg cgcccagctt cggttccagg ggatgagcag gtcgaggaag cgttcccagt
     3121 ccgcgccgcc gcgccgcgcg tagaggaagc gccagggctg cgagttgaac gccgagggcg
     3181 cccagcgggc cgcctcgaac atcgtcagca ggtcctcctc ggggatttgc gatccgtcga
     3241 aggcgcgcgg cgaccagcgc tctaggaaca ggggggtgac cgggtgatca ggctggcggg
     3301 gcatcggctc tcctcatcct ccccatcgcg agatggggag ggggaccatg cgaagcatgg
     3361 tggaggggta caaggcaagg cggctcggct gttccagagc aatcctcaaa tttcggcgcg
     3421 ttctacccct ccaccacatt gcatgtggtc cccctcgcca tctcgcgacg gggaggatga
     3481 tgcaggggtt gcccctccgt gccgccgggg gcatagggcg cgcgacttgc ccgacgccgc
     3541 gggcgccgcc cttcgggagc cttctatgac ctcggacagc ctcgtcgcgg acgcccccgc
     3601 cgccgacgtg cagatcgtcg atgccctcgc ccatgccctc gtcgacggcg cccttcccgg
     3661 cgagcttgcc gacttcaccg acggcgaccg catcgccgcc gcccagttca tcgccgcctg
     3721 cgccgcccgc cgtccgcccg gcatcgccct ggtccgggtc gagccgcagg gcggggcgct
     3781 cggccaccgc cgaatgcgca tcggcatcgt caacgacgac atgcccttcc tggtcgacag
     3841 cgtcgccaac acgctcgccg cccgcaacct caccgtccac cgcctgctcc accccgtggt
     3901 ctgcgtcacc cgcgacgagc agggctgcct gcaggcggtc gagccgctct gcgacgatgc
     3961 cgagcggcgc gagtcgatga tgtatctcga ggtcgaccgc gacgacgcgc ggggccgccg
     4021 cgagatcgtc gtcgacctcc agcgcgtcct cgccgacgtc cgcgccgccg tgaccgactg
     4081 gcgggcgatg caggcgcgga tgcgcgccga cgccgaccag gccgccaacg aggaaggcgc
     4141 cgccctgctg cgctggttcg ccgacggcgc gatgaccctg ctcggttacg aggtggagcg
     4201 ccccgacggc ccgcccaccg acgcgctcgg ccttttccgg attcctggcg agcccaccga
     4261 ccccggcggc gccctcggcg cgatgcgcta tttcgagcag ggcggggacg tccccctgct
     4321 cgccaaggcc gagcgccggg cgtcggtcca ccggcgggtg ccgctcgacc tcgtcgtcgt
     4381 cccggtccgc gagggcggca aggtcaccgg gatcggagtc catgccggcc tgtggaccag
     4441 cgaggcgctg agctcgccgg tggaggaggt gccgctgctg cgcaagcggc tgcagcagct
     4501 cgagcgcgag ttcgggttcg atccgtccgg gcacagcggc aaggcgcttc gccacgccgt
     4561 caccgccttg ccgcacgacc tgctcgtcag cctcagcgtc gaggcggtgc gcgagctggt
     4621 caccaccgcc atgtcgctcg ccgaccggcc gcggcccacc ctctcgctgg tccgctcgat
     4681 cctcaagggc aacatgttcg ccttcgtctg gctgccgcgc gacgagctga ccacccggcg
     4741 ccggctcgcc atcggcgcga tgatcgagca ggcttcgcag ggccggatct ccaactggtc
     4801 ggtcgagctg ggcgacggcg acctcgccct gatccgctac acgctctacg tcggcccgga
     4861 gaccccgacc cccgacgtgg ccgagctcga ccgccgcctc gacgagatgg tgcggggctg
     4921 ggagccctcg gtcgaggagg cgctcggcga cctggtcggg ccgcagcggg cgacccggct
     4981 cgccatcacc tatctcgacg acttcccgga gggctaccgc ctgaccgtgc cgcccccgga
     5041 ggcggcccag gacatcttgc gcatctgcgc gctcggcggc gcctcccagc gcgacgcgcg
     5101 gctctaccgc caggccgagg acgggccgag gcggcttcgg ctcaacacct accgtctcgc
     5161 cgagatcatc cccctgtccg acgcggtgcc ggtgttcgag aacttcggat tccgggtgct
     5221 cgaggagcat cccaccgcgc tcgacgaggg ccggctcggc tacgtccacg aattcctgct
     5281 cgaggtccgc tccggcgagg ccgcccaggt gctcgaccgc gccggcctgg tcgaacgcgc
     5341 gatcgcggag gtgctcgaag gccgggcgga gaacgacccg ttcaaccagc tgctcgtcgc
     5401 cgccgccctg gcgccgcgcg acgtcgtcct gttccgcgcc tggttccgct atctgcgcca
     5461 gggcgggctt tcctattcga tggtgacggt ggtcgaggcg ctgcggcggg cgcccggagt
     5521 cgcccatggc ctcatcgcct tgttcgacgc gctccacgat ccctcgcacg aggagccgcg
     5581 cgatgcggcc gcggcggcgg ccgagcgggc gatacgcgac ggcctctcgc aggtcgcggc
     5641 gatcgacgag gaccggatcc tgcggctcct ccacgccgtc gtctcggcga cgctccggac
     5701 caacgccttc gcgcccgccg gccgcgaggc gctcgccttc aagctcgaca gcgcccgggt
     5761 tccgaacctg ccgcagccgc tgccctggcg cgagatctgg gtctacagcc cgcgggtgga
     5821 gggcatccac ctgcgcggcg gcccgatcgc ccgcgggggc ctcagatggt ccgaccggcg
     5881 cgacgatttc cgcaccgaga tactcggcct gatgaaggcc cagctcgtca agaacgcggt
     5941 gatcgtcccg accggcgcca agggcggctt ctatcccaag cagctccccc cgatcgccga
     6001 gcgcgacgcc tggctcgccg aggggacgga gagctatcgc atcttcatcc gcgcgctgct
     6061 ttcgatcacc gacaacatcg tcggcaacaa ggtggtccac cccgacggca tcgtcgtcca
     6121 tgacggccag gatccctatt tcgtcgtcgc cgccgacaag ggcaccgcca ccttctcgga
     6181 cgtcgccaac gcgatcgcga ccgagcgcgg cttctggctc ggcgacgcct tcgcgtcggg
     6241 cggcagctac ggctacgacc acaaggcgat ggggatcacc gcgcgcggcg cctggatctc
     6301 cgtccagcgc catttccgcg aactcggaat cgacgtccag acccagccga tccgggtcgc
     6361 cggagtcggc gacaggtcgg gcgacgtgtt cggcaacggc atgctgctgt cgaaatcgat
     6421 caagctggtc gcggccttcg accatcgcca catcttcctc gatcccgatc ccgatcccgc
     6481 ggcgagctgg gaggagcgca accgcctgtt cggcctgccc cgctcgagct gggacgatta
     6541 cgaccgcaag ctgatctcga agggcggcgg gatcttcccg cgcgaccaga aggcgatccc
     6601 gctgtcgccg gaggtccgcg ccctgctcgg cgtggagggc gaggagaccg atcccgccac
     6661 gcttctcacc gccatcctgt cgtcgccggt cgacctgctc tggttcggcg gcatcggcac
     6721 ctacatcaag gccaagagcg agagcaacgc cgaggtcgga gaccccgcca acgacgcgat
     6781 ccgggtcaac ggcgccgagc ttcgggccaa ggcgatcggc gagggcgcca atctcgccat
     6841 cacccaggcc ggccgggtcg agttcgccgg cctgggcggg cgcatcaaca ccgacttcat
     6901 cgacaattcg gccggagtcg actgctccga caacgaggtc aacatcaaga tcccgctcaa
     6961 ccgggagatg gcggaagggc ggctcggcct tgaggaccgc aacgccctcc tcgccgagat
     7021 gaccgacgag gtcgcggcga tcgtgctcga ggacaaccgc ctgcagacgc tggccctgtc
     7081 ggtcgccgag cggagcggcg cgacggggct tccgccgctt ctgagggcga tggagatgct
     7141 cgaggagagc ggccgcctca accgcgccgt cgaaggcctc gaatcgaacg aggagctgat
     7201 gcgccggggg caggagaatc gcggcctcac ccggcccgag cttgccatcc tcctctccag
     7261 ctccaagatg gcgctgcagg ccgcgctcga ggccggcaag atcaccgagg atccgacgct
     7321 gacgccggag ctttgcaacg ctttccccga gacgatgcgc aaccgccacg gcgacgccat
     7381 cctcgcccac cgccttcggc gcgagatcat cgccaccaag atcgccaacc ggctggtcaa
     7441 ccggctcggg atcaccgctc ccttcgcgct cgccgaggaa gagggcgcct cgctcggtca
     7501 ggtcgccgcc gccttcgtcg ccgccgagcg cctcttcgac atgcagggct tctgggagcg
     7561 gctcgacacc gccgccctcc ccgagcaggt ccggctcgag ctgttcgacc aggcgagccg
     7621 cgccctccag ctccacatgg cggacatcct tcgcaacatg tcggcgtcgg ccaagctcgc
     7681 cgagatcgtc gacaatctga agcccgggct ggactcgctc gacgaagcgg tcgagggcct
     7741 gctccgccgc gaggtggcga gcgaggcgag cggtcgccgc cagcgcctga tcgagctcgg
     7801 cgccccggag gacatcgccc acaagatcgt ccggctgttc gagctgaacg gcggggtcgg
     7861 catcgccgcg ctcggcaagc ggctcggcgt cgacgtgatc gagctgacca acgcctatac
     7921 gcggctgggc gaggcgctgg gcctcgactg ggcgcagggc gccgccaacc gattccaggc
     7981 caaggaccag tgggagcggc tcctgaccgc cggcctcgcc cgcgacttcg agcagctccg
     8041 gctcgatttc ctccaccgca agcggggcac cgacccggtc gtggcggtcg accagtgggt
     8101 gctcgcccag ggtccccggg tcgagcagtt ccgccgagtc gtcgagcgag tccgcaacgc
     8161 cccggtcacc accgccccga tgctcgccca gatcgccacc caggcgcggg tgctgctggg
     8221 gcgctgaccg tcatgccagc gaaggctggc ctccatgcgg agtcggcatg gaccccactt
     8281 tcgctggggt gacgagaagg atcagccgct ctatcagcac cgccatggcg cgcggcggcg
     8341 agagacgggg catcagccgg cgcaccctgc tggtcggcgg gggcgccggc gccggtttgg
     8401 tcctcgcctg ggcgctgtgg ccgcggacgc tcgagccgaa ccttcgcgcc ggcgagggcg
     8461 agcatgtctt caacgccttc ctcaagatcg gcaccgacgg ccggatcgtc gtggccgtgc
     8521 ctcaggccga gctgggccag ggcagctgga cggcgctccc gcagatcctg gccgacgagc
     8581 tcggcgccga ctggcgcacc gtcggggtcg agccggcgcc gctcgggccc gtctacgcca
     8641 accggctgct cggggagggt gaggacgacg gctcgttcgc gggcggcgtc ggccgctggc
     8701 gggcgcgcaa tttcgaaggg ggggaggcgg cgatgctgac cgccggctcg acgtcggtgc
     8761 gggcgttcga gcggcccctc cgcgaggccg gcgcaggagc gagggcgctg ctgatgaagg
     8821 ccgcggcgag ccgctggggg gtcgactggg agacgctcga caccgccgcc ggcttcgtcc
     8881 tggaagggga ccgccggctc gccttcgccg agctcgcgga ggccgccgcc gaggagacgc
     8941 ttccagccta tctgccgatc cgcggaggaa tcgacaaccg cctcgccggc cagccgctgc
     9001 cgaggctcga cctgccctcg aaggtcgacg gctcggcgcc gttcgccggc gacgtcaggc
     9061 tgcccgagat ggtccatgcc tcggtccgtg ccgggccgac cgaggacagc cgcctcgccg
     9121 gctgggaccg gaccgcggcg ctggcggtgt cgggcgtgct cggcgtcttc gacgccccgg
     9181 gctgggtcgc ctgcgtggcg aacaattggt gggcggcgga ccagggcgtg gaggcgctcc
     9241 gggcgcgctg gcacgtcccg cgtccgccgc tgacggcgga gagcatcgac tccgccctgg
     9301 tcgaagcgct ggagcgcgac gaggggagcc gcgtcttcag ccgcggcgac ctcgccgacg
     9361 cgttcgaggg cggaaacgtg ctgcgcggcc attatcgcgc cggcccggcc cccagcgccc
     9421 ccctcgagac cttgacggcg acggcgcggc tgaccggcgg ccggctcgag atctgggcgc
     9481 cggcccaggc gccgggcctt gcgcgggcgg cggcggcccg ggtggccggg ctgggcgagg
     9541 ccgaggtgac cgtctacccg accctgatcg gcggcggcta cggccgcaag ctggagaccc
     9601 gcgcgatcga gcaggcggcg atgctggcga tgcggacccg gcgtcccgtg cagctcgcct
     9661 ggtcgaggct cgaggaaagc gtccaggacg ggttccgggc cccggcgcgg gcgcaattgc
     9721 tggcccggct ggacgagggc ggcgtcatcc tcggctggca ggcccgaatc gccgcgcccg
     9781 acaccggcgc cgagctcgag gcgcggctgc gcggcggccg caccgccatg gccgcgatcg
     9841 gggatccgac cgccggagcg gtgccgccct acgcgatccc cgcggtggcg gtcgactatc
     9901 tgcccgccga gaccggcctc aggatcggcg cctggcgctc gggcgcgcac agctattgcg
     9961 ctttcttcac cgagagcttc atggacgagc tggcgcgaat ctccggcatc gagccgctgt
    10021 cgttccggat gcagatgctc ggcgacaacc cgcggctggc acaatgcctg acgacggcgg
    10081 cgaccctggg cggctgggac ggcggaacgc cgggcagcgc tatgggcatc gcctgccaca
    10141 gcgccttcgg aagccatatc gccaccctgg tcgagatcga ggtcacggcc gagcagagga
    10201 tccgcgtgct tcgcgcgatc tgcgcggtcg attgcgggcg gatcgtcaat ccggagatcg
    10261 tccggcagca ggtcgaaggc ggcctcgtct tcggcatcgc cgccgcgacg ggtcaggaga
    10321 tcggcttcga ggacggccgg ccggacctgc gcggcctcgg ccagttcggc ttcccgaccc
    10381 tcgccggctc gcccgacgtc accgttgagc tgctcgacag cgaggaggag cccggcggag
    10441 tcaccgagct cgccgttccg accgcggcgc cggccgtcgc caacgccctc cacgccctca
    10501 ccgggcgcag gctgcggacc cttccgctga gggtgggagg atgacggccc ggcccgccga
    10561 ccacccgaag gtgccgccgc cgaggatcgg cgtgctgctg gtcaacctcg gcacgcccga
    10621 caagcccgag ccggggtcgg tgcggctcta cctcaaggag ttcctgtccg atccgcgggt
    10681 gatcgagata ccgcagctag tctggcagcc gatcctgcgc ggaatcgtgc tgacgacccg
    10741 gccgaggaag tcggcgcacg cctaccggca ggtctggacc ggggaaggct cgccgcttgc
    10801 cgcgatcacc gctcgccagg ccaaggcgct ggccgggagc tggggcgaga acgtcgtcgt
    10861 cgaccatgcc atgcgctacg gcaagccggc gatcgcagcg cggatcgacg ctttgcgggc
    10921 gcagggctgc gagcgcatcc tgatcgcgcc gctctatccg caatattgcg cggcgacgac
    10981 ggcgacggcc aacgacgagg ccttcctgca tctgcggcgc ctgcgttggc agccggcggt
    11041 caggaccctg cccccttatt acgacgatcc cgcctatatc gcggcgcttg ccgagcggct
    11101 ggcgcaagcc ctcgccggcc tcgacttcga gccgcaggcg atcctcgcca gcttccacgg
    11161 catgccgcag cggacgctcg agctgggcga tccctatcac tgccattgcc gcaagacggc
    11221 gcggctgctg gaggaagcga gcgggcgcga gcttcgcgtc gtcttccagt cccgcttcgg
    11281 acgggcgaaa tggctgcagc ccgcgaccga cgagaccctc gccgcgctgc cggcggaggg
    11341 cgtcacccgg gtcgcgctgc tcgcccccgg cttcgccgcc gactgcctcg agaccctgga
    11401 ggagctggcc attcgcggcc gcgacacctt cctcgccgcc ggcggcagcg atttcgccta
    11461 tctcccctgc ctcaacgaca gcccgtccgg cctccagatg ctgcgcagca ttttgggccg
    11521 cgagcttgaa ggctggcggg acgcgccgta aggcccgcgc acaacgaaaa aagctcagga
    11581 gagagacatg gcacgcgtag cgatcgtcac cggcggtacc cgaggcatag gcgaggcgat
    11641 cagcgtcgcg ctgcgcgatc agggcgtcac cgtggccgcc aattatgccg gcaacgagga
    11701 gaaggcgcgc gagttcagcg atcgcaccgg catcaaggcg tacaaatggg acgtcagcga
    11761 cttcgacgcc tgcctcgccg gagtcgccca ggtcgagagc gagctggggc cggtcgacgt
    11821 gctcgtcaac aatgccggaa tcacccgcga cggcacgatg aagcggatga cccgccaggc
    11881 ctgggacgag gcgatcgacg tcaacctcgg cggctgcttc aacatggcca aggcggtgtt
    11941 cgaagggatg cagtcgagga agttcgggcg gatcgtcaac atcggctcga tcaacggcca
    12001 ggccggccag tacgggcagg tcaattacgc cgccgccaag tcgggcatcc acggcttcac
    12061 caaggcgctg gcgcaggaag gcgcgcgcgg cggcgtcacc gtcaacgcga tcgcccccgg
    12121 ctatatcgac actgagatgg tcgccgccgt gcccgaggac gtgctccaga agatcgtcgc
    12181 gcgaatcccg gtcggccggc tc
//
LOCUS       DASVQZ010000437         9155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_861556, whole genome shotgun sequence.
ACCESSION   DASVQZ010000437 DASVQZ010000000
VERSION     DASVQZ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861556"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VF547_12320"
     CDS             <1..131
                     /locus_tag="VF547_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX8263650.1"
                     /translation="FEVPEFGVAPGQAAVIYEGSRVLGGGWIEETVPFRAATLAAA"
     gene            complement(194..1375)
                     /locus_tag="VF547_12325"
                     /pseudo
     CDS             complement(194..1375)
                     /locus_tag="VF547_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            1633..2631
                     /locus_tag="VF547_12330"
     CDS             1633..2631
                     /locus_tag="VF547_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX8263651.1"
                     /translation="MQRLVTIDLPRRLSRRLPKAVTELVVGVGITAIFVAIRVALSPL
                     IGGVAPFALSIIAVVLAALVGGWRSGLVSMVLGSLLIWYFVLPPERSFALETAATALT
                     LAAGMLTEAILLLALGLYQREVRAGQTERARRINFLGHALREMDHRTKNNFQIVTSLL
                     TLQANRATSDEVKTALGEAIERLKAVSAVYEALAPSSQGLAAVRLQDQLQEICDQIRR
                     GILPDGITLSTELEPMLVPHETAVSIGIIVNELVTNACKHAFADGVGSIRVRAWREPG
                     AAVIEVEDDGRGFVPGAAKPGLGSRLVTAFLQRLKGRSDVRSSPEGTVHRLRLPLD"
     gene            2631..3128
                     /locus_tag="VF547_12335"
     CDS             2631..3128
                     /locus_tag="VF547_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8263652.1"
                     /translation="MAPRSAGILLYRRDSGGARILLVHPGGPFWSGRDEGAWMIPKGL
                     VEPGEDPVAAALREFEEELGARPAGEPRPLCTVRQAGGKWVEAFALEGDFDCAGLKSN
                     RFTIEYPPRSGQFRDFPEVDDARWFTLAEARSKILKSQAPILDALEALLLNSAPRFGG
                     EGNRA"
     gene            complement(3217..4071)
                     /locus_tag="VF547_12340"
     CDS             complement(3217..4071)
                     /locus_tag="VF547_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX8263653.1"
                     /translation="MPELRFVQVDAFADKPFTGNPAAVIPLTEWLPDETLQAIALENN
                     LSETAFTVPLGDGQADYELRWFTPTTEVDLCGHATLASGHALMGERQSLEFRTRKAGH
                     LTVAREGDGYAVSLPRTNVKRATEAQLRDVVARDGASIVDIITHDGASDSAPPELYLS
                     TDGVEGVALLLFPDEAAVRACRPDMKRLAEIDYMAIVTAPGAPGAGYDVVSRVFVPAW
                     GVDEDPVTGAAHAALMPFWTNRLERQHFTAYQASRRGGHLTVRREHGDRAVLGGKCVT
                     VIEGVFRF"
     gene            complement(4182..5165)
                     /locus_tag="VF547_12345"
     CDS             complement(4182..5165)
                     /locus_tag="VF547_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8263654.1"
                     /translation="MSGAHPRHEAHGTLTRRAALASVSVALFLLVLKVYAAWATGSVA
                     MLGSLADTGLDVLASLITLYGVRIAAEPADLDHRYGHGKAEALAALAQVGIIAFSALG
                     IGWRALDRLLGQAPTANAEHGIAVSLAAIAATLGLLAYQRRIIARTGSVAIGTDYVHY
                     QSDLLLNLSVIAALVLDQYAGLTGADPVFGILIALWLAWGAWRASSRAVDQLMDREWP
                     EEKRRRFVEVAARHPELKGLHDLRTRTSGARDFVQFHVWMDPAMTIAAAHEVIDEVEA
                     KLEKEFPGCEILIHIDPEGQVDQPGNLLVETDLTNLNHQGSASPDDPIRSS"
     gene            complement(5162..5740)
                     /gene="pdxH"
                     /locus_tag="VF547_12350"
     CDS             complement(5162..5740)
                     /gene="pdxH"
                     /locus_tag="VF547_12350"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX8263655.1"
                     /translation="MADDPHALFEEWLTEARLAEPNDPTAMALATADGDGRPAVRMVL
                     MKGHDERGFVFYTNLDSRKGGELADNPRAALLFHWKSLRRQVRIEGPVEPVSDAEADA
                     YFATRGRDSQLGAWASDQSRPLESRALFEARYDGVRRRFEGVDVPRPPRWGGWRVKPE
                     RIEFWNDRAHRLHERRLFTRAAEGWSEGLLYP"
     gene            5898..6860
                     /locus_tag="VF547_12355"
     CDS             5898..6860
                     /locus_tag="VF547_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8263656.1"
                     /translation="MADLYSQLGVKRDASEAEIKKAYRKLAKQLHPDKNKDNPKASER
                     FSKVTQAYDILTDKDKRARYDRGEIDDEGVARAPFGYGGGGGGAGPRPGPGGFGRSPG
                     PDFGGGEEIDLSDLFDGLFGGAGQRRGGGGFSGFGRGRTAPPPQKGADTAYRLPISFE
                     DAATLKEQRVALAGGKTVSIKLPPGVEEGARIRLAGQGQPGPGGNGDAIVTISIKPHR
                     FFSRDGDNIRLDLPISLDEAVLGAKVKVPTVDGPVMLSVPAGTSSGKVLRLKGKGFTA
                     RNGQRGDQLVTLMVEVPVDDAELARFVEGWSRRGKGNPRAALGV"
     gene            6895..7860
                     /locus_tag="VF547_12360"
     CDS             6895..7860
                     /locus_tag="VF547_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8263657.1"
                     /translation="MQDISPESPEARRKRLARAKARFGKIRSRMKPGEYPFEVIKRVA
                     VGVYNDGFIHAGNLAYLALMSLFPFVIVAAAVARIFGQTEEGLAAVNGILQTMPPDVA
                     EVLRKPISDVINARSGGLLWLGALVGLWTTGSFIETIRDIIRRAYGVTFSRPFWEYRL
                     GSVGMIIGAVIVAMVAFSLSVLLSGVQEFIVGLIPRGEELVGLVTLFRVVPALMLFGS
                     LYLIFYSLTPKRYRTRGCPKWPGPLFVTVWWLATTALLPVVLSGLGGYDLTYGSLAGV
                     IIGLTFFFVVGLGVVIGAELNAALAETPEEALKEGEEPFYKRESI"
     gene            7857..8660
                     /gene="fabI"
                     /locus_tag="VF547_12365"
     CDS             7857..8660
                     /gene="fabI"
                     /locus_tag="VF547_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX8263658.1"
                     /translation="MTGLMDGKRGLIMGLANDKSLAWGISKRLAEQGAQLAFSYQGEA
                     LQKRVGPLAAEVGSDFMIECDVADMADLDRLFETLKARWETIDFVVHAIGFSDKNELR
                     GKYVDTSLDNFLMSMNISAYSFTAVARRARDMMPNGGALLTLSYYGAEKVIPHYNVMG
                     LAKAALETSVKYLAMDLGPENIRVNAISAGPIKTLAASGIGDFRYILKWNEYNSPLRR
                     NVTIEDVGGAGLYLLSDLASGVTGEIHHVDAGYNVVGMKAEDAPDIATT"
     gene            8663..>9155
                     /locus_tag="VF547_12370"
     CDS             8663..>9155
                     /locus_tag="VF547_12370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX8263659.1"
                     /translation="MLVRPEAAEDAEGIRAVHLAAFPTAAEADLVARLHEDFESEISL
                     VAERSGEIVGHVLLSRMTVSGGGRGYRALGLGPVGVKPGAQGSGLGTALIRAALGLAE
                     TLGEEVVFVLGEPDYYARFGFSAAAAAPFASPYAGPYFMALWLRSDLDAPADGSAAYA
                     PAFG"
BASE COUNT         1415 a         3102 c         3237 g         1401 t
ORIGIN      
        1 ggttcgaggt cccggaattc ggcgtcgccc cgggccaggc cgcggtcatc tacgaagggt
       61 cgagggtgct cggcggcggc tggatcgagg agacggtgcc cttccgggcg gcgaccctcg
      121 ccgcggcgta aagaagggtc ccggcgaccg ccagggccgc gagcacggcc cacagcacct
      181 ccggcggata attggggttg gtgttcctta tggcggccca gacgcagatg gcgacgccgg
      241 cggccatcat cgcccacacc agggctgtcg gccgctcgtg cttcggcagc gaggcgatgc
      301 agatcgagta gacgatgagg cgggcgagcg tgctcaccgc cgcaagcacc gcgaagctgc
      361 cgctcaccgc aagcagcgcg atcagggcgc ccatgaaggc gattgagttg accggcgtcg
      421 catagcgtgc gtcgattcgt ccgaaccatc ccggaagcag cccgtcccgg cccatcgcgt
      481 aggtcgtgcg ggtcgtcccg gtgaggccgc cgctcacatt gccgaggatc gagcagaccg
      541 cgacgaccgt cagggcgaga ccgccggcgg cgccgaacaa ggccgttccg aaggcgagca
      601 gcggcgcctc cttcccctcg cccggctcca tcaccgcgac gaaggcgagc tggatcagga
      661 aatagagagc ggcggtcgac acgatcgtgg cgacgagggc acgcgggatg gtgcgttgcg
      721 ggtcggccgt ttcgccggcg ggcacgacgc tgttctcgaa gccgacgaag gcatagagga
      781 cgaggagcgc cgcggcctcg aactgggtga aggggggcgg accctcgacc ggatcgatcg
      841 aggctccggc gacgaccagc ccggccagcg ccacgacgag caggggcgcc gccttgagca
      901 gggtgagcgc gtcgagcagc cgaacggcgc gtttcacccc gaccgcgttg acggcgacca
      961 gggcggcgac cacggcgact atggtggcgc cgcgggggat ggggtccgcc agcggcggcc
     1021 agagcttgcc gagataggtg acgaggaccg tcaggttcgc ggccagcgcc gccgaccgcg
     1081 cgacgtagta gagccagccg gcctggaacg aggcgacggg gccgaaggcg gcggcgtaga
     1141 cgaccggtcc gccggatacg cgatgatggc cggcgacgcg tgcgaagggc agcgcgacca
     1201 tcaggacgag caggccgaaa agcgggaaga ggtaggggct gaaggcgccg aacttctcgt
     1261 acagggtgcc gggaagcgcg aagatcccgg cgccgaccac tccgttgaac gacaggaaag
     1321 cggagccgaa gaagccgatg tcgcggcgga ggggctcgtc cttgtccgcg gtcattgcac
     1381 cctctcacgc cggtccgatt ccggttctgg ccgccgcgcc ccttccccgc aaggccttgt
     1441 aatccgagcc ggttccgccg gtctcggacg cccgctgggc cggggccgcc gcgagcgcgc
     1501 cggcgggttc ggcggaaatc gccgctgccg gtgcaacgga ttgattttga aggtttaact
     1561 ttaatcagcg tggttcaggt ggccgctggt ggcccgattg tggtagcagc ggcgccgggg
     1621 aaacggggga aagtgcagag gctcgtcacc atagacctgc cgcggcgcct gtcgcgtcgg
     1681 ctgccgaaag cggtgacgga gctcgtcgtc ggcgtgggca tcaccgcgat cttcgtcgcg
     1741 atccgagtcg ccctgtcgcc gctgatcggc ggagtggcgc ccttcgcgct gtcgatcatc
     1801 gcggtcgtcc tcgccgccct cgtcggcggc tggcgcagcg gactggtgtc gatggtgctc
     1861 ggctcgctgc tcatctggta cttcgtgctg ccgcccgaac gaagcttcgc gctcgaaacc
     1921 gccgcgacgg cgctcactct cgcggcgggg atgctgaccg aggcgatcct cctgctcgcc
     1981 ctcggcctct accagcgcga ggtccgcgcc ggccagacgg agcgggcccg gcggatcaac
     2041 ttcctcggcc acgcgctcag ggagatggat caccgcacca agaacaactt ccagatcgtc
     2101 accagcctcc tgacgctgca ggccaaccgc gcgaccagcg acgaggtgaa gacggccctc
     2161 ggcgaggcga tcgagcggct gaaggcggtc tcggcggtct acgaagctct cgcgcccagc
     2221 agccagggcc tggcggcggt gcggctgcag gaccagctgc aggagatctg cgaccagatc
     2281 cggcgcggca tcctgcccga cggaatcacg ctctcgaccg agctcgagcc gatgctggtc
     2341 ccgcacgaga cggcggtctc gatcgggatc atcgtcaacg agctcgtcac caacgcctgc
     2401 aagcatgcct tcgccgacgg cgtcgggtcg atccgggtgc gggcgtggcg cgagcccggc
     2461 gccgccgtca tcgaggtcga ggacgacggc cgcgggttcg tcccgggcgc ggccaagccg
     2521 ggcctgggca gccggctcgt caccgccttc ctccagcgtc tgaagggccg cagcgacgtc
     2581 cgctcgtctc cggagggcac ggtccaccgg ctgcgcctgc cgctggactg atggcgccgc
     2641 gcagcgccgg catcctgctc taccgccgcg attcgggcgg agcgcgcatc ctcctggtcc
     2701 atcccggcgg gccgttctgg agcggccgcg acgagggcgc gtggatgatc cccaagggcc
     2761 tggtcgagcc gggcgaggac ccggtggccg ccgccctgcg cgagttcgag gaggagctgg
     2821 gagccaggcc ggcgggcgag ccgcggccgc tctgcacggt ccgccaggcc ggcggcaaat
     2881 gggtggaggc cttcgcgctg gaaggcgatt tcgactgcgc gggcctgaag agcaaccgct
     2941 tcacgattga gtatccgccc aggagcggcc agttccggga ctttcccgaa gtcgacgacg
     3001 cgcgctggtt cacgctcgcc gaggctcggt cgaaaatcct gaagagccag gcgccgatcc
     3061 tggatgcgct ggaggcgctt ctcctaaatt ccgccccgcg ctttggcggg gaggggaacc
     3121 gcgcgtagcg cggtggaggg gttctacgga cttcgcgaga accccctcca ccagccttcg
     3181 gctggtcccc ctcccctgca aatggggggg aggcatttag aagcggaaga ccccctcgat
     3241 caccgtcacg catttgccgc cgagcacggc acggtcgcca tgctcccggc ggacggtgag
     3301 atggccgccc cgccggctcg cctggtaggc ggtgaagtgc tggcgctcga gccggttggt
     3361 ccagaagggc atcagggcgg cgtgggcggc tccggtcacc ggatcctcgt cgacccccca
     3421 ggccgggacg aagacccggc tgaccacgtc gtagccggcg cctggcgccc ccggggcggt
     3481 gacgatcgcc atatagtcga tctccgccag ccgcttcatg tccggccggc acgcccgaac
     3541 cgccgcctcg tcggggaaga gcaggagcgc gacgccctcg accccgtcgg tgctgaggta
     3601 gagctccggc ggcgccgaat cgctcgcccc gtcatgggtg atgatgtcga cgatggaagc
     3661 gccgtcgcgg gcgacgacgt cgcgcagctg cgcctcggtc gcgcgcttga cgttggtccg
     3721 cggcaggctg accgcatagc cgtcgccctc gcgcgcgacg gtgaggtggc cggccttgcg
     3781 cgtccggaac tcgaggctct ggcgctcgcc catcagcgca tggccgctcg ccagggtggc
     3841 gtggccgcac aggtcgacct cggtcgtcgg ggtgaaccag cgcagctcgt aatcggcctg
     3901 gccgtcgccg agcgggacgg tgaaggcggt ctccgagagg ttgttctcga gcgcgatcgc
     3961 ctggagggtc tcgtcgggaa gccattccgt caacgggatg acggcggccg gattgccggt
     4021 gaacggcttg tcggcgaagg cgtcgacctg gacgaagcgc agctcaggca tttggggtat
     4081 ctcctttcgc tcccgctcat cccgagtaac cgctgagcga agtcgaagcg gcgtatcgaa
     4141 ggaccccctt cgatacggcc ttcgccaggc tcagtcccta ctcaggatga gcggattgga
     4201 tcatcgggcg atgccgatcc ttggtggttg aggttcgtca agtccgtttc gaccaggaga
     4261 ttccccggct ggtcgacctg gccctcggga tcgatgtgga tcaggatctc gcagccgggg
     4321 aattccttct ccagctttgc ttcgacctcg tcgatcacct cgtgggcggc ggcgatcgtc
     4381 attgccgggt ccatccagac gtggaactgg acgaagtcgc gggcgccgct ggtccgggtc
     4441 ctgaggtcgt gcaggccctt cagctcgggg tggcgggcgg cgacctcgac gaagcggcgt
     4501 cgcttctcct ccggccattc ccgatccatc agctgatcga cggcgcggct cgaggcgcgc
     4561 catgcccccc aggccagcca cagggcgatg aggatcccga agaccgggtc ggcgccggtc
     4621 aggccggcat attggtcgag gaccagcgcc gcgatcaccg acaggttgag cagcaggtcg
     4681 ctctggtaat ggacatagtc ggtgccgatc gcgaccgatc cggtgcgggc gatgatccgg
     4741 cgctggtagg cgagcaggcc gagcgtggcg gcgatcgccg ccagcgacac ggcgatgccg
     4801 tgttcggcgt tcgccgtcgg cgcctggccg agcagccggt cgagcgcccg ccagccgatt
     4861 cccagcgccg agaaggcgat gatgccgacc tgggcgaggg cggccagggc ctcggccttg
     4921 ccgtggccgt agcggtggtc gaggtccgcc ggctcggcgg cgattcgcac tccgtagagc
     4981 gtgatcagcg aggcgagaac gtcgaggccg gtgtcggcga gcgagccgag catcgccacc
     5041 gatccggtcg cccaggcggc atagaccttc agcaccagca ggaagagggc gaccgagacg
     5101 ctggccagag ccgcgcggcg agtgagagtg ccgtgcgcct cgtgacgcgg atgagcgccg
     5161 ctcacgggta gagcagccct tcgctccaac cctccgcggc gcgggtgaac agccggcgct
     5221 cgtgtagccg gtgggcgcga tcgttccaga attcgatccg ctcgggcttg acccgccagc
     5281 cgccccagcg cggcggcctg ggcacgtcga cgccctcgaa gcggcggcgc acgccgtcgt
     5341 agcgcgcctc gaacagggcc cggctctcga gcggccgcga ctgatcggac gcccacgccc
     5401 cgagctgtga gtcgcggccg cgggtcgcga aataggcgtc ggcttcggca tcgcttaccg
     5461 gctcgacggg gccctcgatg cgcacctgcc ggcgcagcga cttccagtgg aagagcaggg
     5521 cggcgcgggg gttgtcggcg agctcgccgc ccttgcggct gtccagattg gtgtagaaga
     5581 cgaagccgcg ctcgtcgtgg cccttcatca gcaccatccg caccgccggg cgcccgtcgc
     5641 cgtcggcggt ggcgagggcc atggcggtcg gatcgttcgg ctcggcaagc cgggcctcgg
     5701 tcagccattc ctcgaacagg gcgtgggggt cgtcggccat ggtggcctct tgccgtctgt
     5761 gcgccggggg cacaagcatg gcctcccgtc gggacgacga tggccgcttg cgccgccgag
     5821 gtgcgttacc catctgatac agcgttgccg cacgcaacag tggacttttc aaccggtctg
     5881 gagaacgaag cgcaaagatg gcggacctct attcacagct gggcgtgaaa cgcgacgcga
     5941 gcgaggccga gatcaagaag gcctatcgca agctcgccaa gcagctccat ccggacaaga
     6001 acaaggacaa tcccaaggcc tcggagcgct tctcgaaggt cacccaggcc tacgatatcc
     6061 tcaccgacaa ggataaacgc gcgcgctacg atcgcggcga gatcgacgac gagggagtcg
     6121 ctcgcgcccc gttcggctat ggcggcggcg gcggcggcgc ggggcctcgg ccgggcccgg
     6181 gcgggttcgg ccgctcgccc gggcccgatt tcggcggcgg cgaggagatc gacctttccg
     6241 acctgttcga cggcctgttc ggcggcgccg gccagcgccg cggcggcggc ggcttcagtg
     6301 gattcggacg gggccgtacc gcgcccccgc cccagaaggg cgccgacacc gcctatcgcc
     6361 tgccgatcag cttcgaggac gcggcgaccc tgaaggagca gcgagtcgcg ctcgccggcg
     6421 gcaagacggt ctcgatcaag ctcccgccgg gagtcgagga gggggcccgg atccggctcg
     6481 ccggccaagg ccagcccggc ccggggggca atggcgacgc catcgtcacc atctcgatca
     6541 agccccaccg cttcttctcc cgcgacgggg acaatatccg gctcgatctg cccatctcgc
     6601 tcgacgaggc ggtgctcggc gccaaggtca aggttccgac cgtcgacggc ccggtgatgc
     6661 tgagcgtccc ggccggcacc agctcgggca aggtgctgcg cctcaagggc aagggattca
     6721 ccgcccggaa cgggcagcgc ggcgaccagc tcgtcaccct gatggtcgag gttcccgtcg
     6781 acgacgccga gctcgcccgc ttcgtcgagg gctggagccg cagaggcaaa ggaaacccgc
     6841 gggcggccct gggcgtctag cggctcatga accgtctttg gtcatagccg cctgatgcag
     6901 gacatctccc ccgaatcgcc ggaagccagg cgcaagcgtc tcgcccgggc caaggcgcgg
     6961 ttcggcaaga tccgcagccg gatgaagccg ggggaatatc cgttcgaggt gatcaagcgg
     7021 gtcgcggtcg gcgtctacaa cgacggtttc atccacgccg gcaacctcgc ctacctcgcc
     7081 ttgatgtcgc tcttcccctt cgtcatcgtc gccgccgcgg tggcccgcat cttcggccag
     7141 accgaggagg ggctggccgc ggtcaacgga atcctgcaga cgatgccgcc cgacgtcgcc
     7201 gaggtgcttc gcaagccgat cagcgacgtg atcaacgcgc gctccggcgg cctgctctgg
     7261 ctgggcgccc tggtcgggtt gtggaccacg ggcagcttca tcgagaccat ccgcgacatc
     7321 atccgccgcg cctatggggt caccttcagc cggccgttct gggaatatcg gctcggctcg
     7381 gtcgggatga tcatcggggc ggtgatcgtc gcgatggtcg ccttcagcct ttccgtgctc
     7441 ctctccggcg ttcaggagtt catcgtcggg ctgatccccc gcggggagga gctggtgggc
     7501 ttggtcacgc tgttccgggt ggtgccggcg ctgatgctgt tcgggtcgct ctacctcatc
     7561 ttctattcgt tgacccccaa gcgctaccga acccgggggt gccccaaatg gcccggtccg
     7621 ctgttcgtca ccgtctggtg gctggcgacc acggcgctgc tcccggtcgt cctgtcgggg
     7681 ttgggcggct acgatctcac ctatggcagc ctggccgggg tgatcatcgg gcttaccttc
     7741 ttcttcgtcg tcggcttggg cgtggtgatc ggggccgagc tcaacgccgc gctggcggag
     7801 acgccggaag aagccctaaa ggagggtgaa gagcctttct acaagcggga aagcatatga
     7861 ccggattgat ggacggaaag cgcggcctga tcatgggcct cgccaacgac aagtcgctgg
     7921 cctgggggat atcgaagcgg ctcgccgagc agggggccca gctcgctttc tcctaccagg
     7981 gcgaggcgct gcagaagcgg gtcggcccgc tcgccgcgga ggtcggctcc gacttcatga
     8041 tcgagtgcga tgtcgccgac atggccgatc tcgaccggtt gttcgagacg ctcaaggcgc
     8101 gctgggagac gatcgacttc gtcgtccacg ccatcggctt ctcggacaag aacgagcttc
     8161 gcggcaaata tgtcgacacc agcctcgaca atttcctgat gtcgatgaac atatccgcct
     8221 acagcttcac cgcagtggcc cgccgagccc gcgacatgat gccgaacggc ggcgccctgc
     8281 tcaccttgtc ctattacggc gccgagaagg tcatcccgca ttacaacgtc atgggcctcg
     8341 ccaaggcggc gctcgagacc agcgtcaaat atctagccat ggacctcggt cccgagaaca
     8401 tccgggtcaa cgccatctcg gcaggcccga tcaagaccct ggcggcgagc ggaatcggcg
     8461 acttccgcta catcctcaag tggaacgaat ataattcccc tctgcgccgc aacgtcacca
     8521 tcgaggacgt cggcggcgcc ggcctctacc tcctctccga cctcgcctcg ggcgtcacag
     8581 gcgagatcca ccacgtcgac gccggctaca acgtcgtcgg catgaaggcc gaggacgccc
     8641 ccgacatcgc caccacgtga ccatgctcgt tcggccggaa gcggcggagg acgccgaagg
     8701 catacgcgcc gtccatctcg cggctttccc gaccgcggcg gaagccgatc tcgtcgcccg
     8761 cctgcacgag gatttcgagt ccgaaatctc gctggtggcg gagcggtcgg gcgagatcgt
     8821 cggccatgtc ctgctcagcc gcatgacggt ctcgggcggc ggacgaggtt atcgcgcgct
     8881 cggcctgggg ccggtcggag tcaagccggg cgcgcagggc agcggcctcg gcaccgcgct
     8941 gatccgcgcc gcgctgggcc tagcggagac cctcggcgag gaggtggttt tcgtcctcgg
     9001 cgagcccgat tattacgccc gtttcggctt ttcggcggcg gcggcggccc ccttcgcctc
     9061 cccttatgcg ggaccctatt tcatggcgct gtggcttcgc tccgacctcg acgccccggc
     9121 ggacggctcc gcggcctacg cccccgcctt cggca
//
LOCUS       DASVQZ010000438         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_668483, whole genome shotgun sequence.
ACCESSION   DASVQZ010000438 DASVQZ010000000
VERSION     DASVQZ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668483"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VF547_12375"
     CDS             complement(<1..1273)
                     /locus_tag="VF547_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8263660.1"
                     /translation="MNSELKIAPELRQDPRPRSAVSTGVGMAGLAGLFAWVAAARTFG
                     LSDENGALIALLACGVPMVLWSLFVDKVHRSPTTGIDWDSPARPLSQSLDYSVVKIAG
                     LWATWAAIGFLYCVGRWYWDGIYLFSMEVLGLAAAPLLALSIPYVVWIDRRLVEPRDG
                     AWHFGQLLIGQSALVDREAVYEHLRCWAVKGFFMAFMIAIVPGNWTGTVAPSGQEITF
                     NLSNLTRWLITVMFMIDTVFATVGYALTMKPLDAHIRSVNPYAAGWTAALICYPPFVL
                     MGGGGPLDYHSGTMGENGWSYWLDGYPLLMALVGFLLVVLTAIYAWATVAFGIRFSNL
                     THRGVLTHGPYAWTKHPAYVSKNLFWWLATLPFLATTGNLTDVIRNSFIMGVVAGVYY
                     WRARTEERHLSADPAYRDYAEWMERNAPLPRL"
     gene            1471..2112
                     /locus_tag="VF547_12380"
     CDS             1471..2112
                     /locus_tag="VF547_12380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8263661.1"
                     /translation="MLSNECHEETIFSFSQRAPQPPERRRDARHLTILRVGALIGPNG
                     RELCLIRNISAGGLMAHVYSHHATGDAVAVELKSNEAVSGRVVWADQSNVGIEFDSPI
                     DVEEMLSSQAALDNGWRPRLPRVEVDRLATLRCGARLYGVNTRDISQGGVKIETDQPF
                     DVGREVVLTMERFRPLAGVVRWCHEGLAGIAFNHLIPFHELMQWLRPEAAAKR"
     gene            2188..2274
                     /locus_tag="VF547_12385"
     tRNA            2188..2274
                     /locus_tag="VF547_12385"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2222..2224,aa:Tyr,seq:gta)
     gene            2387..2457
                     /locus_tag="VF547_12390"
     tRNA            2387..2457
                     /locus_tag="VF547_12390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2419..2421,aa:Gly,seq:tcc)
     gene            complement(2462..3322)
                     /locus_tag="VF547_12395"
     CDS             complement(2462..3322)
                     /locus_tag="VF547_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8263662.1"
                     /translation="MRKALRWAGIGLGAVVALLLAAALIIFAWSEIVIRTSYSAAPET
                     LPPPTAAQAAQAPRLARVLGCLSCHGEGLKGRVMFDAPGVARVFAPNITEVAAKSSDQ
                     QLAAGIRQGIGHDGRGLFIMPSPMYSRLTDGETASLIGWIRSLPRAEGQGRGLSAGPI
                     GRLGLVIGTFRPAPKLVAEYRRQVPIALGPKHEPARHLAATACSECHGPSLMGGSAGP
                     DEKAPDLRIVAGYDPAQFKVLMRTGRPPTGRDLGLMTTVAKEDLSHLTDAEIDALYAY
                     LVARAKRLGS"
     gene            3390..4241
                     /locus_tag="VF547_12400"
     CDS             3390..4241
                     /locus_tag="VF547_12400"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8263663.1"
                     /translation="MNRLRLPLLAVLLAGCTAEVRESDLIRPLRAGGLSADAVAAAAP
                     AYTLAEHRLPAPDGANLYAVHLRQPGAGATILYFGGNGYTIGRFGAWTASVFAPLGVD
                     LIIVDHRGYGLSEGKPSASAMEDDALAAFDHARRLVGPAGRIVVHGHSLGSFIAGHVA
                     ANRAVAGVVLESSATTTEDWVKTGTPGIAKPFIRKVEIDPTLRGRGNLSVVPRIDEPL
                     LLLVGAKDGTTPPSLSQRLYAASPLPEGRKTLTVVAGAGHADVMTKPAAAGAYRAFLA
                     KLAAPAS"
     gene            4377..5387
                     /locus_tag="VF547_12405"
     CDS             4377..5387
                     /locus_tag="VF547_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263664.1"
                     /translation="MRNLILLAGTAALALAAPAAAEKGGKGGGHGHGGGKPAKVEGGG
                     GHGGGHGGGKPARAERGGGQGGGHAMRAPKPSKAERQAFRAERPSRMERQVLRESRRD
                     DRFVRRAAEDRRDLRRDFRRDDDFRPRERFVGFDRACPPGLARKGNGCLPPGQAKKRF
                     GIGERLDRAWFDGYRMPAAYQSFYHDTPDYSYRYDDDGYIYRTDRGSDLISAIIPLFG
                     GGFGVGQPLPAGYDVYNVPMQYRDTWHDNDESLYRYGDNAIYRVDSESGLIEGIVALL
                     AGDLNVGQPLPDGYDAYNLPLDYRDEYVDDDEHLYRYADGNIYQVDAKTQMIQAIVEM
                     LA"
BASE COUNT          888 a         1910 c         1841 g          791 t
ORIGIN      
        1 tgaggcgcgg caagggcgcg ttgcgctcca tccactcggc ataatcgcga taggccgggt
       61 cggcggagag gtggcgctcc tcggtccggg cgcgccagta atagacgccg gcgacgaccc
      121 ccatgatgaa gctgttgcgg atcacgtcgg tgaggttgcc cgtcgtcgcc aggaaaggca
      181 gggtcgcgag ccaccagaac aggttcttcg acacataggc cggatgcttg gtccaggcgt
      241 aggggccgtg ggtcaggact ccgcggtggg tcaggttgga gaagcggatt ccgaatgcga
      301 ccgtcgccca ggcgtagatc gcggtcagca ccaccagcag gaagccgacc agcgccatca
      361 gcagcgggta gccgtcgagc cagtagctcc agccgttctc gcccatcgtc ccggaatggt
      421 agtcgagcgg cccgccgccg cccatcagca cgaagggcgg atagcagatc agcgccgcgg
      481 tccagcccgc cgcataggga ttgacggagc ggatatgggc gtcgagcggc ttcatcgtca
      541 gcgcgtagcc gaccgttgcg aagaccgtgt cgatcatgaa catgacggtg atcagccagc
      601 gggtcaggtt gctcaggttg aaggtgatct cctggcccga cggagcgacc gtcccggtcc
      661 aattgccggg gacgatggcg atcatgaagg ccatgaagaa gcccttgacc gcccagcagc
      721 gcagatgctc gtagaccgcc tcgcggtcga cgagggccga ctggccgatc agcagctggc
      781 cgaaatgcca ggctccgtcg cgcggctcga ccagtcggcg gtcgatccag acgacatagg
      841 ggatggacag agcgagcagc ggcgcggcgg cgagccccag cacttccatc gagaacaggt
      901 agatgccgtc ccaataccag cggcccacgc aatagaggaa gccgatggcg gcccaggtcg
      961 cccacaggcc ggcgatcttg acgacgctgt agtcgagcga ctggctcagc ggccgggccg
     1021 ggctgtccca gtcgatgccg gtggtcgggc tgcgatggac cttgtcgacg aacagggacc
     1081 acagcaccat cggcacgccg caggcgagga gcgcgatcag cgccccgttc tcgtcggaga
     1141 gcccgaaggt gcgggcggcc gccacccagg cgaacagccc ggcgaggccg gccatgccga
     1201 ccccggtgct caccgcggag cggggacgcg gatcctggcg cagctcgggc gcgatcttca
     1261 gctcggagtt catcgagacg cgggcttaag gccgaatggt aagcaagccg tgaacggccg
     1321 ccccaaggag cgggtccagt gcagacttcg cgccaattcg gaccaattcc gctcggcccc
     1381 gtaagaggtt cttaaatctc tggcgcgata tggcttttcg cggcgggcga agtggggcgt
     1441 cgacgccgtg agcgatggcg gggagggacg atgctgtcga acgaatgtca cgaggagacg
     1501 atcttctcct tctcgcaacg cgcgccccag ccgcccgagc ggcggcgcga cgcgcggcac
     1561 ctcaccatcc tgcgcgtcgg cgctctgatc ggacccaatg gccgcgagct ctgcctgatc
     1621 cgcaacatct ccgccggcgg gctgatggcc catgtctatt cgcaccacgc caccggcgac
     1681 gccgtcgcgg tcgagctcaa gagcaatgag gccgtttccg gccgggtggt ctgggccgac
     1741 cagtccaatg tcggcatcga gttcgattct ccgatcgacg tcgaggagat gctgtcgagc
     1801 caggcggcgc tggacaatgg ctggcggccg cggctgccgc gggtcgaggt cgaccggctg
     1861 gcgaccctgc gctgcggcgc ccggctctac ggggtcaaca cgcgcgacat cagccagggc
     1921 ggggtcaaga tcgagaccga ccagccgttc gacgtcggcc gcgaagtggt tctgacgatg
     1981 gagagattcc gcccgctcgc cggagtggtg cgatggtgcc acgagggcct cgccggaatc
     2041 gccttcaacc acctgatccc gttccacgag ctgatgcagt ggctgaggcc ggaggcggcc
     2101 gccaagcgct gaaccgcccg gcttgcgggc gttgacagcc cccgcccctt tcgccaatgg
     2161 gtcgccctcc cccactgggt gcctcctgga caggtggccg agtggttaaa ggcagcagac
     2221 tgtaaatctg cccgggtttc ccgtacgctg gttcgaatcc agccctgtcc accaaccgcc
     2281 gctcaagctt cgcccaaggt gcggccggca agccgtccat ccggtgccgg tgacggcggg
     2341 cttgcccgcg gaagcccggt tcgcctaaag ggcgaagctg gcgcaggcgg gtgtagctca
     2401 atggtagagc agaagccttc caagcttacg acgagggttc gattcccttc acccgctccg
     2461 ctcagctccc cagccgcttc gccctcgcca ccagataggc atagagggcg tcgatctcgg
     2521 cgtcggtgag gtggctgagg tcctctttgg ccaccgtcgt catcagcccg aggtcgcggc
     2581 cggtgggcgg gcggccggtc cgcatcagga ccttgaactg ggccggatcg tagccggcga
     2641 cgatcctcag gtcgggcgcc ttctcgtcgg gaccggccga accgcccatc agcgagggcc
     2701 cgtggcattc ggagcaggcc gtcgccgcga ggtgccgcgc cggctcgtgc ttcggaccga
     2761 gcgcgatcgg gacctgcctt cgatattcgg ccacgagctt cggcgccggc cggaatgtcc
     2821 cgatcaccag cccgagacgc ccgatcggcc cggcgctgag cccccgcccc tggccttccg
     2881 ctcgaggcag gcttcgaatc cagccgatca gggaagccgt ctcgccgtcg gtcaggcggg
     2941 agtacatcgg cgagggcatg atgaagagcc ccctgccgtc gtggccgata ccctggcgga
     3001 tccccgcggc gagctgctgg tcgctcgact tcgccgcgac ctcggtgatg ttgggagcga
     3061 agaccctggc gacgcccggg gcgtcgaaca tcaccctccc cttcagccct tcgccatggc
     3121 agctgaggca gccgagcacc cgcgccagcc gaggcgcctg ggccgcctgc gccgccgtcg
     3181 gcggaggcag ggtctccggc gctgcggaat agctcgttcg aatgacgatc tccgaccagg
     3241 cgaagatgat cagcgccgcc gccagcagca gggcgaccac ggctccgagg ccgatgccgg
     3301 cccaccgcaa cgctttcctc atggcgaaac cccctgtttg ccgccaaata ggtgtcacga
     3361 tccgttcctt ccgacaagag tcgaccagga tgaaccgtct tcgcctcccc ctcctcgccg
     3421 tcctgctcgc cggctgcacc gccgaggtgc gggaaagcga cctcatccgg cctcttcgcg
     3481 ccggcggcct ctccgccgat gcggtcgccg cggcagcgcc ggcctacacg ctcgccgagc
     3541 accgcctccc cgcgccggac ggggccaacc tctacgccgt ccatctgcgc cagccgggcg
     3601 ccggggcgac gatcctctat ttcggcggca acggctacac catcggccgc ttcggcgcct
     3661 ggaccgccag cgtcttcgcc ccgctcgggg tcgacctgat catcgtcgac catcgcggct
     3721 acggactcag cgagggaaag ccgagcgcga gcgcgatgga ggacgatgcc ctcgccgcct
     3781 tcgaccatgc gcgccggctg gtcggcccgg cgggacgcat cgtcgttcac ggccattcgc
     3841 tcggcagctt catcgccggc catgtcgccg ccaaccgcgc ggtggcgggc gtggtcctcg
     3901 aaagctcggc gacgaccacc gaggactggg tgaagaccgg cacgccgggc atcgccaagc
     3961 cgttcatcag gaaggtcgag atcgacccga cgctgcgcgg ccgcggcaac ctctccgtcg
     4021 tcccgcgcat cgacgagccg ctgctcctgc tggtcggcgc gaaggacggc accaccccgc
     4081 cgagcctgtc gcagcggctc tatgcggcct cccccctgcc cgagggccgc aagaccctga
     4141 cggtggtggc cggcgccggc catgccgacg tgatgacgaa gccggcggcg gcgggcgcct
     4201 atcgcgcgtt cctggcgaag ctggccgcgc ccgcttcata gggcgttcag gcgccgacag
     4261 gcggcgttca gcttggcggg accagtggag ccggcaatcc ggacgagacg tgcgctcgcg
     4321 gcgggaacgg caatcgccgc ctgcccgttc atctgtggaa ccacgggagc tttctcatgc
     4381 gtaacctcat ccttctcgcc ggcaccgccg cgctcgccct cgccgcgccg gccgcggccg
     4441 aaaaaggggg caagggcggc ggccacggcc atggcggcgg caagccggcc aaggtcgagg
     4501 gcggcggcgg ccatgggggt ggtcatggcg gcggcaagcc ggcccgagcc gaacgtggcg
     4561 gcggccaggg cggcggccat gcgatgcgcg cgcccaagcc gtccaaggcc gagcgccagg
     4621 cgttccgcgc cgagcgtcct tcgaggatgg aacggcaggt cctccgcgaa agtcgtcggg
     4681 acgaccgctt cgtccgccga gccgccgagg accgccggga ccttcgccgc gatttccgcc
     4741 gcgacgacga tttccgtccc cgcgagcgct tcgtcggctt cgaccgcgcg tgcccgccgg
     4801 gactggccag gaagggcaat ggctgcctgc ccccgggcca ggcgaagaag cgcttcggca
     4861 tcggcgagcg gctcgaccgg gcctggttcg acggctaccg catgccggcc gcctaccaga
     4921 gcttctatca cgacacgccg gactattcct atcgctacga cgacgacggc tacatctacc
     4981 gcaccgaccg gggcagcgac ctgatcagcg cgatcatccc cctgttcggc ggcggcttcg
     5041 gagtcggcca gccgctgccg gccggctacg acgtctacaa cgtcccgatg cagtatcgcg
     5101 acacctggca cgacaatgac gagtcgctct accgctacgg cgacaacgcc atctaccggg
     5161 tcgattcgga atccgggctg atcgagggca tcgtcgccct gctcgccggc gacctcaacg
     5221 tcggccagcc gctgcccgac ggctacgacg cctacaatct tccgctcgac tatcgcgacg
     5281 aatatgtgga cgacgacgag catctctacc gctatgccga cggcaacatc taccaggtcg
     5341 acgccaagac gcagatgatc caggcgatcg tcgagatgct cgcctgaccg aaaaggaccc
     5401 agcatgcgcc aagcagctct cgccgccgcc
//
LOCUS       DASVQZ010000439         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_955617, whole genome shotgun sequence.
ACCESSION   DASVQZ010000439 DASVQZ010000000
VERSION     DASVQZ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955617"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VF547_12410"
     CDS             complement(<1..774)
                     /locus_tag="VF547_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263665.1"
                     /translation="MTKSRRMLAAPVALAALTGLTPPPTADEEPPVPVELAQLDPTAP
                     SEAAPAAPSDPAPAEPAKVAQAEPRLPPAVVGDVFYSSDSDRSEVTRAAIELGLGDAR
                     DERSIGVRVERARYNPTGRGWESRDRVFLRGAAKLGHWQLRGRVGTDGDGVIGAVSLN
                     DDSRFRKEFFVERDIVETRQGLDRGLYSTFAGAAVDLPVDDRNVFTALAGVQAFTGDN
                     VRLHLRGSYIHVVEPKIGLSAQLRGRYFRSSRPGEFDYYS"
     gene            complement(771..>2139)
                     /locus_tag="VF547_12415"
     CDS             complement(771..>2139)
                     /locus_tag="VF547_12415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8263666.1"
                     /translation="EQGIAGRSAGPVREAGASGKCGLQFAVAAAFAFAWTVFSIWVSK
                     PWLGDLGKLTHPVPALAALTFVAYLPGFMNAFLLSSLLMAPKRRRRRQLCYPDVSILI
                     AAYQEEEAIAGTLRSVRDAGYPGRIEIVVLDDGSADGTSAAVDYCRTALFAETPRIEL
                     RLIRFERNRGKAMALNAGLEQARSELVVTIDADTHLHPGSLAAIVDRLLSDPPDTVAV
                     AGAVLVGNAGESLIAGAQQWDYFHGIAAVKRMQSMYGGTLVAQGAFSIYRRSALLDAG
                     GWPDTVGEDIVLTWALLRKGHRIGYAEGAVAWTHAPPTLAELARQRTRWARGMIEALA
                     HHRPLLFKPRLTTMFIWWNLLFISLDLTFTLVFIPGLVLALFGFYWLAGPITLLVLPL
                     AALWNLVIFRIQMRMLRRSGIEMKRSLAGFVLFALAYPLLMQPVSLWGYVAELTGRKK
                     EWGGT"
BASE COUNT          348 a          713 c          785 g          293 t
ORIGIN      
        1 cgaataataa tcgaactccc cggggcggct gctccggaaa tagcggccgc gaagctgggc
       61 gctgaggccg atcttcggct cgaccacgtg gatgtagctg ccgcgcaggt gaagccggac
      121 gttgtcgccg gtgaaggcct ggacgccggc gagggcggtg aagacgttgc ggtcgtcgac
      181 cgggaggtcg acggcggcgc ccgcgaaagt ggaatagagg ccccgatcca ggccctgccg
      241 ggtctcgacg atgtcgcgct cgacgaagaa ttcctttcgg aacctcgagt cgtcgttgag
      301 cgagacggcg ccgatgacgc cgtcgccgtc ggtgccgacg cggccgcgca gctgccagtg
      361 gccgagcttc gcggcgccgc gcaggaagac gcggtcgcgc gattcccagc cccggccggt
      421 cggattgtag cgcgcccgct cgacccgcac gccgatcgag cgctcgtccc gggcgtcccc
      481 gaggccgagc tcgatcgccg ccctcgtcac ctcgctgcgg tcgctgtcgg acgagtagaa
      541 gacgtcgccg accaccgccg ggggaagccg cggctcggcc tgagcgacct tggccggctc
      601 cgccggagcg gggtcggacg gcgcggccgg agcggcctcg gacggcgcgg ttggatcgag
      661 ctgggcgagc tcgaccggca cgggcggctc ctcatcggcg gtcggcggcg gggtcaggcc
      721 ggtgagggcg gcgagagcga ccggggccgc gagcatccgt cgggatttcg tcatgttccg
      781 ccccattcct tctttcttcc ggtgagctcg gcgacatagc cccagagcga gaccggctgc
      841 atgagcagcg gataggcgag ggcgaagagg acgaagcccg ccaggctgcg cttcatctcg
      901 ataccggagc gcctgagcat ccgcatctgg atccggaaga tcaccaggtt ccacagagcc
      961 gccagcggca gcacgagcag ggtgatcggc cccgccagcc agtagaagcc gaaaagggcc
     1021 agcaccaggc cgggaatgaa caccagggtg aaggtgagat cgagcgagat gaacagcagg
     1081 ttccaccaga tgaacatcgt cgtcagccgc ggcttgaaca gcagcggccg gtggtgcgcg
     1141 agcgcctcga tcatgccccg cgcccagcgg gtccgctgcc gggcgagctc ggcgagggtg
     1201 ggcggcgcat gggtccaggc gacggcgccc tccgcatagc cgatccggtg gcccttgcgg
     1261 agcagcgccc aagtgaggac gatgtcctcg ccgaccgtgt ccggccagcc gccggcgtcg
     1321 aggagggcgc ttcgccggta gatcgagaag gcgccctggg cgacgagcgt gccgccatac
     1381 atgctctgca tccgcttcac cgcggcgatg ccgtggaaat aatcccattg ctgggcgccc
     1441 gcgatcaggc tctcgccggc attgccgacc agcacggcgc ccgccaccgc caccgtgtcc
     1501 ggcgggtcgg agagcagccg gtcgacgatc gcggcgaggc ttcccggatg gaggtgggtg
     1561 tcggcgtcga tggtgacgac gagctcgctt cgcgcctgtt cgaggccggc gttgagggcc
     1621 atcgccttgc cccggttgcg ctcgaagcgg atcaggcgaa gctcgatccg gggcgtttcg
     1681 gcgaagagcg ccgtccggca atagtccacc gcggcggacg tgccgtcggc cgaaccgtcg
     1741 tcgagcacga cgatctcgat tcggcccgga tagccggcgt cgcggacgct ccgcagcgtc
     1801 ccggcgatcg cctcctcctc ctgataggcg gcgatcagga tgctgacgtc cggatagcag
     1861 agctgccgcc gccgccggcg cttcggcgcc atcagcaggc tggacaggag gaaggcgttc
     1921 atgaagccgg gcaggtaggc gacgaaggtg agagcggcca gcgccgggac cggatgggtc
     1981 agcttgccaa gatcgccgag ccagggcttg gacacccaga tgctgaacac cgtccaggcg
     2041 aaggcgaagg cggcggcgac cgcaaattgc agaccgcatt tgccgcttgc cccggcctcg
     2101 cggacgggac cagccgacct gccagcaatg ccctgttcc
//
LOCUS       DASVQZ010000440         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_862416, whole genome shotgun sequence.
ACCESSION   DASVQZ010000440 DASVQZ010000000
VERSION     DASVQZ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862416"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1386
                     /locus_tag="VF547_12420"
     CDS             190..1386
                     /locus_tag="VF547_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX8263667.1"
                     /translation="MGPAEPLTPGPSPRRERASRSEETDMLEVKADALEASFEALERE
                     DQEIAALREEMAQLKSRVDAQTATARPALSGAKGERSGFVENYLRKGLEAGVELKAVT
                     GTSDSAGGYAVPEEIDAEIDRLLTSISPIRAIANVVKVGTSGYRKLVTTGGTPSGWVS
                     ETAARPETDTPAFVEIAPPFGDLYANPAASQAMLNDAAFDVEAWLASEIATEFARAEG
                     AAFVSGSGINRPKGFLSAPVSAAGDATRPFGTLQYVASGAAGGFAASAPQDRLIDLVQ
                     ALRPPYRQGAVFVMNSATASLIRKFKTSSGAFLWQPSLSVGQPDTLLGYPVVEAEDMP
                     DVAADSLSIAFGNFKAGYLIAERAETQILRDPYTHKPFVHFYATKRVGGQVSNSEAIK
                     LMKFSA"
BASE COUNT          243 a          504 c          608 g          237 t
ORIGIN      
        1 gggcgctgcc ttcgaggtga gcgaacatgc tgcgcagccg ctcgacgcgg gctgcatcga
       61 ataggtcctg gtggcgctcg gcgccgtcgg gatcgaaccg cattcgtaga gtttagggcc
      121 gcggccggcg cggcggaagg acttcctctc ccgacaggga gggggaacgg ggtgaggcgc
      181 ggcctttttt tgggcccggc cgaacccctc accccgggcc cctctccccg acgggagagg
      241 gcgtcacgtt ctgaggagac tgatatgttg gaagtgaagg cggacgcgct cgaggcctcg
      301 ttcgaggcgc tggagcggga ggatcaggag attgcggcgc tgcgcgagga gatggcgcag
      361 ctcaagagcc gtgtggacgc ccaaacggcg acggcgcggc cggcgctttc gggcgcgaag
      421 ggcgagcggt cgggcttcgt cgagaattat cttcgcaagg ggctggaagc gggggtcgag
      481 ctcaaggccg ttaccggcac gtcggacagc gcgggcggct atgcggtgcc cgaggagatc
      541 gacgccgaga tcgaccggct gctgacatcg atctcgccga tccgggcgat cgccaacgtg
      601 gtgaaggtcg ggacctccgg ctaccgcaag ctggtgacca ccggcggcac gccatcggga
      661 tgggtcagcg aaacggcggc gcggccggag accgacacgc ccgcctttgt cgagatcgcc
      721 ccgccgttcg gcgacctcta cgccaatccg gcggcgagcc aggcgatgct caacgacgcg
      781 gccttcgacg tcgaggcgtg gctcgcgagc gagatcgcga ccgagttcgc gcgggcggaa
      841 ggggcggcct tcgtctcggg atcgggcatc aaccggccca agggcttcct ttcggcgccg
      901 gtctcggcgg cgggggatgc gacgcggccg ttcggcacgc tgcaatatgt cgcgtccggc
      961 gccgccggcg gctttgcggc ttcggcgccg caggaccggc tgattgacct cgtccaggcg
     1021 ctgcggccgc cctaccggca gggcgcggtg ttcgtgatga attcggccac ggcatcgctg
     1081 atccgcaagt tcaagacctc gagcggcgcc ttcctttggc agccgtcgct gagcgtcggg
     1141 cagccggaca ctttgctcgg ctatccggtg gtggaggcgg aggacatgcc ggatgtggcg
     1201 gcggacagcc tctccatcgc gttcggcaac ttcaaggcgg gctatctgat cgccgagcgg
     1261 gcggagacgc agatcctgcg cgatccgtac acgcacaagc cgttcgtcca cttctacgcg
     1321 accaagcggg tcggcgggca ggtgtcgaac agcgaggcga tcaagctgat gaagttcagc
     1381 gcctgaggcc gcgcgtcgcc cggttctcct cacggaggcc gggcggcgat gccgtcggcg
     1441 gcggagcgcc gcgtggagag agggacgatc atgatcgaca cggacgcgct gacgctgccc
     1501 tccgaagcgg cggcggaggc ggcgactttc ctgcgcgccg gcggcgccgg cgaggagacg
     1561 ctgatcgccg agctgaccca ggcggcggcc tg
//
LOCUS       DASVQZ010000441         4047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_955941, whole genome shotgun sequence.
ACCESSION   DASVQZ010000441 DASVQZ010000000
VERSION     DASVQZ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955941"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF547_12425"
     CDS             complement(<1..206)
                     /locus_tag="VF547_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263668.1"
                     /translation="MGDRYADGAARLADSATGRLLPPIDAPAAARIVATRYTGKARIA
                     AVGRTSAEAPPLDLRRKVAAWRVT"
     gene            complement(542..1183)
                     /locus_tag="VF547_12430"
     CDS             complement(542..1183)
                     /locus_tag="VF547_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263669.1"
                     /translation="MKALAGSAAAASLLLAACGPKPLQLPADPVDRAATCAVVAAATA
                     RTGVADLKAPLPLPAQGRIFHYALLAASEGGEFKADTANRVNKRMRELQEAITGGKWQ
                     DLEPPCETAFPLAGKTRIELPAGRLDAQLACEELAEFTSVALDGEEIHYGNELAAYRR
                     MRSDLNDALGPGLRARAGADLEAQSRERKRALAAAAQLGSPMAVLDACAERFD"
     gene            complement(1256..2653)
                     /gene="lpdA"
                     /locus_tag="VF547_12435"
     CDS             complement(1256..2653)
                     /gene="lpdA"
                     /locus_tag="VF547_12435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8263670.1"
                     /translation="MSETEFDVIVIGAGPGGYVAAIRAAQLGLKTACAESRETLGGTC
                     LNVGCIPSKALLHASELFEEAAHGTLAKWGIRATGVELDLEAMHSGRREAVKGLTGGV
                     EFLFKKNKVTWLKGHAKFTGPNAIDVAGQAYSAKSIVIATGSSVTPLTGVEIDQQRIV
                     DSTGALELEKVPGHLVVIGGGVIGLELGSVWRRLGAKVTVVEYLDQILPGMDGEVRKE
                     AAKIFKKQGFDIRTSTKVTGAQKKGEGVVLTVEPAKGGEAQAIEADVVLVSIGRRPNT
                     DGLDLEAAGVRPNPRGQIEVDHDFRTGVEGVYAIGDVTPGPMLAHKAEDEGIAVAEIL
                     AGQVGIVNHDVIPSVVYTFPEIASVGKTEEELKEAGVEVKIGKFPMLGNSRAKTNRET
                     DGFVKVIADARTDRVLGVHIIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEA
                     VKEAAMAVTGKPIHV"
     gene            complement(2738..4039)
                     /gene="odhB"
                     /locus_tag="VF547_12440"
     CDS             complement(2738..4039)
                     /gene="odhB"
                     /locus_tag="VF547_12440"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX8263671.1"
                     /translation="MATDVKVPTLGESITEATLGQWLKMPGDAVKADEPVASLETDKV
                     AVEAPSPVDGVMGDQLVAVGDTVAVGAAIARIESGSGMPATPKTEQATPAEVPVNPEG
                     AGENVELRESSADDGDESPHLTLSPAVRRIVLEHHLDPSRIKGTGKDGRLTKDDVLAA
                     AKAKEAAPVPPPSSPATAAAQPAPAQASAPASAGAQRAAGERREERVRMTRLRQTVAR
                     RLKEAQNTAAMLTTFNDVDMTAVMEARNRYKDLFEKKHGIRLGFMSFFVKAAVLAARD
                     VPAVNAYIEGEEIVYHDYVDVSVAVSAPNGLVVPVIRDAQALGFAEIEKTIADFGKRA
                     KDGTLTMADMEGGTFTISNGGVFGSLISTPIINPPQSAVLGMHRIEERPVVRGGQIVV
                     RPMMYLALSYDHRLVDGREAVTFLVRMKEALEDPTRLLIDL"
BASE COUNT          538 a         1447 c         1395 g          667 t
ORIGIN      
        1 atcgtcaccc gccaggcggc gaccttgcgg cgcaggtcga gcggcggcgc ctccgccgag
       61 gttcggccga ccgcggcgat acgcgccttg ccggtgtagc gcgtcgcgac gatgcgcgcg
      121 gccgcggggg cgtcgatcgg cggcagcagc cggcccgtgg ccgagtccgc caggcgcgcc
      181 gccccgtcgg cgtagcgatc acccatttcg gcccgtcggg ccggggctcg aggcggagcg
      241 aggcgaccgg ccgcggaccg atcgccggcg gcgtcgggac gaggccggag ggaagcgcgg
      301 gcgcctcggc gatcagcccc tcgccgcgca ccgtctcgat cggccgcgcc accatgacca
      361 ggccggacag cgtccagaaa aggaaaggaa ggccgacgat ccagcccagc cagatgtgcc
      421 agcgcctgag cctgtgccga attgtcgcca cgcttcctcc gtcccgccga aagcaagggc
      481 gcagccttgc gcgccgagcg gaataaggaa aggcggattc ccgctttcga cggaaatgac
      541 gctagtcgaa ccgctcggcg caggcgtcga gcaccgccat cggcgatccc agctgcgccg
      601 ccgccgccag cgcccgcttg cgctcgcgcg actgcgcctc cagatcggcg ccggcgcgcg
      661 cgcgcaggcc agggccgagc gcgtcgttga ggtcgctgcg catccgccgg taggcggcga
      721 gctcgttgcc gtaatgtatc tcctcgccgt cgagcgcgac gctcgtgaac tcggccagct
      781 cctcgcacgc gagctgggcg tcgagccgtc ccgccgggag ctcgatccgg gtcttcccgg
      841 cgagggggaa ggcggtttcg cagggcggct cgaggtcctg ccacttgccg ccggtgatcg
      901 cctcctgcag ctcgcgcatg cgcttgttga cgcggttcgc cgtgtccgcc ttgaactcgc
      961 cgccctcgga tgcggcgagc agggcataat ggaagatcct gccctgcgcg ggcagcggca
     1021 gcggcgcctt gaggtcggcc accccggtcc gcgccgtcgc ggcggcgacg acggcgcagg
     1081 tggcggcgcg gtcgaccggg tcggcgggaa gctggagcgg cttggggccg caggcggcga
     1141 gcaggagcga ggcggccgcg gcggatccgg ctagggcttt caacggcggg actcccaatt
     1201 cgttacggcc tgccaacgga gcaaagaccc gtcccgcctt ccgcgtcaac tccgctcaga
     1261 cgtggatcgg cttgccggtc accgccatcg cggcttcctt gacggcttcc gaatgggtgg
     1321 gatgggcgtg gcaggtatag gcgatgtcct cgctggtcgc tccgaactcc atcgcctgcg
     1381 ccgcctgggc gatcatcgtc ccggcgaccg aggcgatgat gtggacgccg agcacccggt
     1441 cggtgcgcgc gtcggcgatc accttgacga agccgtcggt ctcccggttg gtcttggcgc
     1501 ggctgttgcc gagcatcggg aacttgccga tcttcacctc cacgcccgct tccttcagct
     1561 cctcctccgt cttgccgacc gaggcgatct ccgggaaggt gtagacgacg ctggggatga
     1621 cgtcgtggtt gacgatcccg acctggccgg cgaggatctc ggcgaccgcg atgccctcgt
     1681 cctcggcctt gtgggcgagc atcgggcccg gcgtgacgtc gccgatcgcg tagacgcctt
     1741 cgactccggt cctgaaatcg tggtcgacct cgatctggcc gcgcgggttg ggccgtaccc
     1801 cggcggcctc gaggtcgagg ccgtcggtgt tcggacgccg gccgatcgag accagcacca
     1861 cgtccgcctc gatcgcctgc gcctcgccgc ccttggccgg ctcgaccgtc agcacgacgc
     1921 cttcgccctt cttctgggcc ccggtgacct tggtcgaggt gcggatgtcg aagccctgct
     1981 tcttgaagat cttcgccgcc tccttgcgga cctcgccatc cattcccggc aatatctggt
     2041 cgagatattc gacgaccgtc accttggcgc cgagccgccg ccagaccgag cccagctcga
     2101 gtccgatcac gcccccgccg atcaccacca gatggcccgg caccttctcc agctccagcg
     2161 ccccggtgga gtcgacgatc cgctgctggt cgatctcgac tccggtcagc ggagtcaccg
     2221 acgagccggt ggcgatgacg atgctcttcg ccgaataggc ctggccggcg acgtcgatcg
     2281 cgttcgggcc ggtgaacttc gcgtggccct tgagccaggt caccttgttc ttcttgaaca
     2341 ggaactcgac gccgcccgtc aggcccttca ccgcctcgcg gcggccggaa tgcatcgcct
     2401 cgaggtcgag ctcgacgccc gtcgccctga tcccccattt ggcgagcgtt ccgtgcgcgg
     2461 cctcctcgaa cagctcggac gcgtggagaa gggccttcga ggggatgcag ccgacgttga
     2521 ggcaggtgcc gccgagcgtc tcgcggctct cggcgcaggc ggtcttcagc cccagttgcg
     2581 cggcgcggat cgcggcgacg tagccgcccg gcccggctcc gatcacgatc acgtcgaatt
     2641 cagtctctga cattcacttc atccttattc gtcatgccag cgagggctgg catccatgcc
     2701 ggctcagaat ggaccccggc cttcgccggg gtgacggtta gaggtcgatc agcaatctcg
     2761 tcgggtcttc cagcgcttcc ttcatccgca ccaggaaggt gaccgcctcg cggccgtcga
     2821 ccaggcggtg gtcgtagctg agcgccaggt acatcatcgg ccggacgacg atctggccgc
     2881 cgcgcacgac cggccgctcc tcgatcctgt gcatgccgag cacggcggac tggggcggat
     2941 tgatgatcgg ggtcgagatc agcgagccga acaccccgcc gttggagatg gtgaaggtgc
     3001 cgccctccat gtcggccatg gtcaaggtgc cgtccttggc gcgcttgccg aagtccgcga
     3061 tcgtcttctc gatctcggcg aagcccagcg cctgggcgtc gcggatgacc gggacgacca
     3121 ggccgttcgg cgccgagacc gcgaccgaca cgtcgacata gtcgtgatag acgatctcct
     3181 cgccctctat gtaggcgttg accgccggca cgtcgcgcgc ggccagcacc gccgccttca
     3241 cgaagaagct catgaagccg aggcggatgc cgtgcttctt ctcgaacagg tccttgtagc
     3301 ggttcctcgc ctccatcacc gccgtcatgt cgacgtcgtt gaaggtggtc agcatcgccg
     3361 ccgtgttctg ggcctccttg agccggcggg cgacggtctg gcgaagccgg gtcatccgaa
     3421 cccgctcctc gcggcgctcg ccggcagctc tttgagcgcc ggcggaggcc ggggccgagg
     3481 cttgcgccgg cgccggctgg gcggcggccg tcgccgggga gctcggtggc gggacgggcg
     3541 ccgcctcctt ggccttggcg gcggccagga cgtcgtcctt ggtcagccgc ccgtccttgc
     3601 cggtgccctt gatccgcgac gggtcgagat gatgctcgag cacgatccgc cgaaccgccg
     3661 gcgacagcgt gagatgcggg ctctcgtccc cgtcgtccgc cgaggactcg cggagctcga
     3721 cattctcgcc cgcgccctcc gggttgaccg gaacctccgc cggcgtcgcc tgctccgtct
     3781 tcggggtcgc cggcatcccg gatccgctct cgatccgggc gatggcggcg ccgaccgcga
     3841 ccgtgtcgcc gacggcgacg agctggtcgc ccatcacgcc gtcgacgggc gagggcgcct
     3901 cgacggcgac cttgtcggtc tccaggctcg ccaccggctc gtcggccttc acggcgtcgc
     3961 cgggcatctt cagccactgg ccgagggtgg cctcggtgat cgattcgccg agcgttggga
     4021 ctttgacgtc ggtcgccatg tcgttcc
//
LOCUS       DASVQZ010000442         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_288173, whole genome shotgun sequence.
ACCESSION   DASVQZ010000442 DASVQZ010000000
VERSION     DASVQZ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288173"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VF547_12445"
     CDS             complement(<1..283)
                     /locus_tag="VF547_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8263672.1"
                     /translation="MSEIDETRAPLMEHLIELRRRLIWSFAALGVAFGISFYFAGEIF
                     AFLVQPLIAGFGGPGEGRLVYTKLYEAFFTEVRVALFAAFFLAFPIIAIQ"
     gene            complement(280..624)
                     /gene="tatB"
                     /locus_tag="VF547_12450"
     CDS             complement(280..624)
                     /gene="tatB"
                     /locus_tag="VF547_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX8263673.1"
                     /translation="MFDIGYSELLLIAVVALIVIGPKDLPRVMRTVGQWVGRARGMAR
                     HFRSGIDTMMREAELEEMEKKWREENERILRDHPFADPLADPLALGAPAEPPSAAEVE
                     APPAGDRPGERP"
     gene            complement(652..921)
                     /locus_tag="VF547_12455"
     CDS             complement(652..921)
                     /locus_tag="VF547_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8263674.1"
                     /translation="MGGLSIWHWLVVGVIILLLFGKGRFSDMMGDVAKGLKSFKKGMS
                     EDDAPPVGASPPPPPPPRITEARPVDPAPAERTHEADAATERREP"
     gene            complement(1201..1791)
                     /gene="scpB"
                     /locus_tag="VF547_12460"
     CDS             complement(1201..1791)
                     /gene="scpB"
                     /locus_tag="VF547_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX8263675.1"
                     /translation="MIGMDDSLRAVEAVLFASPEPLTAEDIAAHVGGEVDVAAALAEL
                     ARHYDGRGIELVERGKRWHFQTAPDLAFILRRERDETRKLSRAAVETLAIVAYHEPVS
                     RAEIEAIRGVQISKGTLDVLMEAGWVRPAGRREAPGRPLIYATTPAFLAHFGLESRRD
                     LPGLDDLKAAGLLDPIDAAMEQLGLAGLEDEEEADA"
     gene            complement(1880..2650)
                     /locus_tag="VF547_12465"
     CDS             complement(1880..2650)
                     /locus_tag="VF547_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX8263676.1"
                     /translation="MGGDDAFDEFETPAPVREADRLTLDLDGWEGPLDLLLTLARSQK
                     VDLARISILALVEQYLAFIADAKKLKLEIAADYLVMAAWLAYLKSCLLLPRDQEADPS
                     PEELAMRLQLRLQRLSAMREAGARLLGRDRLGRDVFLRGAPEGLRVVRKSLWQAELYD
                     LIAAYGSVRARSEPAFHIVARRPVMTLEEALLRVERMIGTKLEWTRIEAFLPDTQDGE
                     FRKSALASSFVAALELARKGRVEIEQKGAFEPLYIRAA"
     gene            2799..4799
                     /locus_tag="VF547_12470"
     CDS             2799..4799
                     /locus_tag="VF547_12470"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX8263677.1"
                     /translation="MRTPLLLASGLAALVLASGPGLGQRPQPGVAAALPSQPRGGLAA
                     FRSDAELRAYLRRLKRQQEEAAPPFVPQPAPMPPPPPPPPAPATAGADGAVTVTGTRI
                     ARPNLTSSSPIAVVGAEESITNNQEASVDEGDIVKVRGEHLVILRRGRLFTVSLAGGR
                     MRPVDSINAFPPGVSGRGDWYDEMLIAGDRIIVVGYSYARGGTEINRFRLDPAGRLRF
                     EDAWHLKSNDYYSSRNYASRLIGNRLIFYTPLFLDWDEDPLEALPGVRRWRGDGRGAF
                     RRIAAARRVYIAPAMRDSRDADVDTLHSVTSCDLAAAELGCSAVAVLGPASRSFYVSP
                     QAVYLWVSDSWRDGGRRRGPDSFLYRLPFGRERPSAVGARGAPVDQFSFREDGDEGVI
                     NVLLRGRSGGDFMFSPEVSGTGVALARVRFSDLGDGSRELPRSRYRVLPDPGRNAWSF
                     QNRFAGNYVLYGGGAFGQTPGSAKLVVAPLRGGRIAELAVPHSVDRIDLLGTDAIAIG
                     GAPGGLGFSTVELPAGGPRLGDHYLLPDAAEGETRSHAFFFSPDRDSPDGASGTLGLP
                     VARRADPALARFFGSSAAMLFLRRERRRLSRAGELEAQSRGVADDACEASCVDWYGNA
                     RPIFLGGRTFALLGYELVEGELDRGRIREVGRISFAPTERRQ"
BASE COUNT          684 a         1798 c         1675 g          712 t
ORIGIN      
        1 gctggatggc gatgatcggg aaggcgagga agaaggcggc gaacagcgcc acccggacct
       61 cggtgaagaa cgcctcgtag agcttggtgt agaccaggcg gccctcgccg ggcccgccga
      121 agccggcgat cagcggctgc accaggaagg cgaagatctc cccggcgaag tagaagctta
      181 tgccgaacgc gaccccgagc gcggcgaacg accagatcag ccggcgccga agctcgatca
      241 gatgctccat cagcggagcg cgggtctcgt ctatctcgct catggccgct cgcccggcct
      301 gtccccggcg ggcggcgcct cgacctccgc cgccgacggc ggctcggccg gagctccgag
      361 cgcgagcgga tcggcgagcg gatcggcgaa cggatggtcc ctcaagatcc gctcattctc
      421 ctcgcgccat ttcttctcca tctcctcgag ctcggcctcg cgcatcatcg tgtcgatgcc
      481 cgagcggaag tgtcgggcca tgccgcgggc gcggccgacc cactggccga cggtgcgcat
      541 cacccgcggc agatccttcg ggccgatgac gatgagggcg accaccgcga tcagcagcag
      601 ctcggaatag ccgatgtcga acatggcgcc ccttcgcggc ggcgacggag gctagggctc
      661 gcgccgctcc gtcgcggcat cggcctcgtg ggtgcgctcg gcgggcgccg gatcgaccgg
      721 cctggcctcg gtgatgcgcg gcggcggcgg cgggggcgga gaggctccga cgggcggggc
      781 gtcgtcctcg ctcatcccct tcttgaagct cttcaggccc ttggcgacat cgcccatcat
      841 gtcggagaag cggcccttgc cgaagagcag caggatgatc actccgacga ccagccagtg
      901 ccaaatgctc aacccgccca tgatccgtct ccaatggcca atcccgtcat gccggcgaag
      961 gccggcaccc ctagagagaa aaggcccggc ccttcccccc agagccccgg cttgcgccgg
     1021 ggtgacggga ggtacctagt cctcctcgtc ctcactttcc agcccgcagc gcggccgccg
     1081 ggccgatcca ctccgtcatt gggagcgaag cgaagccatc cagtgcggcc tccaggtccc
     1141 cctggatcgc ttcgtcgctc cgctcctcgc aatgacggga aaccgcccgg gggtcggaga
     1201 tcaggcgtcc gcttcctctt cgtcctccag cccggccagc cccagctgct ccatcgccgc
     1261 gtcgatcggg tcgagcaggc cggccgcctt gaggtcgtcg aggccgggca ggtcgcggcg
     1321 gctctcaagc ccgaaatggg cgaggaaggc cggggtggtg gcgtagatga gcggccggcc
     1381 gggcgcctcg cgccgccccg ccggccgaac ccagcccgcc tccatcagca cgtcgagcgt
     1441 ccccttcgag atctggactc cgcggatcgc ctcgatctcg gcgcggctga ccggctcgtg
     1501 ataggcgacg atcgccagcg tctcgaccgc cgcgcgcgac agcttgcggg tctcgtcgcg
     1561 ctcgcgcctg aggatgaagg cgaggtcggg cgcggtctgg aaatgccacc gcttgccgcg
     1621 ctcgaccagc tcgatgccgc ggccgtcgta atggcgcgcg agctcggcca gggccgcggc
     1681 gacgtcgacc tcgccgccga catgcgccgc gatgtcctcg gcggtcagcg gctcgggcga
     1741 ggcgaacagc accgcctcga ccgcgcgcag gctgtcgtcc atcccgatca tttcctcgcc
     1801 tccctgcaag ggacgggccg agggtgggcc aacgcggagc gggctcgatg cccgcgaaac
     1861 gcttcggcgc cagccatttt cacgcggccc tgatgtaaag aggctcgaac gcgcctttct
     1921 gctcgatctc gacccggccc ttgcgggcca gctcgagcgc ggcgacgaag ctcgaggcga
     1981 gcgcggactt cctgaactcg ccgtcctggg tgtcggggag gaaggcctcg atccgggtcc
     2041 attcgagctt cgtcccgatc atcctctcga cccgcagcag ggcctcctcg agcgtcatca
     2101 ccggccggcg ggcgacgatg tggaaggccg gctcgcttcg cgcccgaacc gagccgtagg
     2161 cggcgatgag atcgtacagc tccgcctgcc agagcgactt cctgaccacc ctcagccctt
     2221 ccggcgcgcc gcgcaggaag acgtcgcggc cgagccggtc gcggccgagc agccgcgccc
     2281 cggcctcccg catcgccgac aggcgctgga gcctgagctg gagccgcatc gccagctcct
     2341 cggggctggg atcggcttcc tggtcgcggg ggagcagcag gcaggatttg agataggcga
     2401 gccaggcggc catgacgaga tagtcggcgg cgatctcgag cttgagcttc ttggcgtcgg
     2461 cgatgaaggc cagatactgc tcgaccaggg cgaggatcga gatcctggcc aggtcgacct
     2521 tctgcgaccg cgccagggtc aggagcaggt cgagcggccc ctcccagccg tcgaggtcga
     2581 gggtcagccg gtcggcctcg cgcaccggcg caggcgtctc gaattcgtcg aaggcatcgt
     2641 ccccgcccat cgcccgagac taggggctgg gcggcgcgaa gcgaaggggg attgaagcaa
     2701 ttatgcacag cattttttga ccttcgcagg ccgatttgcc ccttgccgca cgaccttgca
     2761 tgatacaaag tatccagaac tgacgtgagg gagaagccat gcgtaccccc ctgctgctcg
     2821 cttccggcct tgccgccctc gtcctcgctt cggggccggg cctcggccag cggccccagc
     2881 ccggagtcgc cgccgccctc cccagccagc ctcgcggagg cctcgccgcc ttccgctccg
     2941 acgccgagct tcgagcctat ctccgccgcc tcaagcgcca gcaggaggag gcggcgccgc
     3001 ccttcgtgcc gcagcccgcg ccgatgccgc cgccgccgcc gccgccgccg gccccggcca
     3061 cggccggcgc cgacggcgcg gtgaccgtca ccggcacgcg aatcgcccgg cccaacctca
     3121 ccagcagctc gccgatcgcc gtcgtcggcg ccgaggaatc gatcaccaac aaccaggaag
     3181 ccagcgtcga cgagggcgac atcgtcaagg tgcgcggcga gcatctggtc atcctgcgcc
     3241 gcggccgact gttcaccgtc tcgctcgccg gcggccggat gcggccggtc gattcgatca
     3301 acgccttccc gcccggggtc agcgggcgcg gcgactggta tgacgagatg ctgatcgccg
     3361 gcgatcggat catcgtcgtc ggctacagct atgcccgcgg cggaaccgag atcaaccgct
     3421 tccggctcga tccggcgggg cggcttcgct tcgaggacgc ctggcacctc aagtcgaacg
     3481 attattattc gtcgcgcaac tacgcctcgc ggctgatcgg caaccggctg atcttctaca
     3541 cgccgctctt cctcgactgg gacgaggatc cgctcgaggc gctgccggga gtccggcgct
     3601 ggcggggcga cgggcgcggc gccttccggc ggatcgccgc ggcgcgccgg gtctacatcg
     3661 ccccggcgat gcgcgattcc cgcgatgcgg acgtcgacac gctgcacagc gtcaccagct
     3721 gcgacctcgc ggcggcggag ctcggctgct cggccgtcgc cgtgctcggg ccggccagcc
     3781 gaagcttcta cgtctcgccc caggccgtct atctgtgggt ctccgactcc tggcgcgacg
     3841 gcgggcgccg ccgcggcccg gacagcttcc tctaccgcct ccccttcggc cgcgagcgcc
     3901 cctcggcggt cggcgcgcgc ggcgctccgg tcgaccaatt ctcgttccgc gaggacggcg
     3961 acgagggcgt gatcaacgtc ctgctgcgcg gtcggagcgg cggcgacttc atgttctcgc
     4021 cggaggtgtc ggggaccggc gtcgccctgg cgcgggtgcg gttcagcgac ctcggcgacg
     4081 gctcgcgcga gctgccgcga agccgctacc gggtgcttcc cgatcccggc cgcaacgcct
     4141 ggtcgttcca gaaccggttc gccggcaatt acgtcctcta cgggggaggc gccttcggcc
     4201 agaccccggg ctcggccaag ctggtcgtcg ccccgctccg cggcggccgg atcgccgagc
     4261 tggccgtccc ccattcggtc gaccggatcg accttctggg gaccgacgcg atcgcgatcg
     4321 gcggagcgcc gggcgggctc ggcttcagca ccgtcgagct cccggccggc ggcccgcgcc
     4381 tcggcgacca ttatcttctc cccgacgcgg cggagggcga gacgcgcagc cacgccttct
     4441 tcttcagccc ggaccgggac agtccggacg gggcctccgg aacgctcggc ctcccggtcg
     4501 cgcgccgcgc cgatccggcg ctcgcccgct tcttcggaag ctcggcggcg atgctgttcc
     4561 tgcgccgcga gcgccggcgc ctgtcccggg ccggcgagct cgaggcgcag tcgcggggag
     4621 tggccgacga cgcctgcgag gcgagctgcg tcgactggta cggcaatgcg cggccgatct
     4681 tcctcggcgg ccggaccttc gccctgctcg gctacgagct ggtcgagggc gagctcgacc
     4741 gaggccggat ccgggaggtc ggccggatca gcttcgcacc gacggagcgc cggcaatgac
     4801 gcagacggtt gccaccgtcg tgcgcggctg cgtagactcc gccccatgct cggcccgacg
     4861 ctggaaacc
//
LOCUS       DASVQZ010000443         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_570269, whole genome shotgun sequence.
ACCESSION   DASVQZ010000443 DASVQZ010000000
VERSION     DASVQZ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570269"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1417
                     /locus_tag="VF547_12475"
     CDS             308..1417
                     /locus_tag="VF547_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263678.1"
                     /translation="MTGRDDDIAAALPKAPSPAPARRAAAIAEAMRRFDGGWEALSSV
                     PGGRTSAAPAPWRSRFNRPQAGALAGALLVALVALPLAWSSLPDQAREGGGKRTQSPD
                     PASIAAPAAGPASPSADPAPDLAEPVLANPATPPAPAGRPVSASTDEPAADPPGGRSG
                     IAVARSPAEQRDSDLPAPAPAAADAEEIVVTGSRIRNPDLGSPLAVTSIGGEEFDRAG
                     KADRRGDWNACTIDDPRRDLRACRSDVASVARGASGKASAPLAEGLSNAWKGDLEAAV
                     AAFDRAIQLAPRSAAAHLNRGLARRRLGDGDRALADLNRAVTYAPQSARVYYHRSLLL
                     RERGEARRALADERRAVELDADYAELVRAQRRRAR"
     gene            1414..>1847
                     /locus_tag="VF547_12480"
     CDS             1414..>1847
                     /locus_tag="VF547_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8263679.1"
                     /translation="MIAAPRRRFWTSLYFQVLVAIALGVLTGWLFPEFGTSLKPLGDA
                     FIKLVKMIITPVIFLTVTTGIAGMSDLKAFGRVGAKAMAYFLTFSTLALAVGLVVGNL
                     VRPGDGVNADPATLDQGAVATYVDKAHDQSITGFVLDIIPDT"
BASE COUNT          257 a          674 c          647 g          269 t
ORIGIN      
        1 gctcgatggg ctatgacgac gtccttcggg tcgtgtcggc ggcccggggc gaggatccgt
       61 tcggctaccg gtccgagctg atccagctgg tgcggacggc gagctcggcc aggtcgatgg
      121 acgatggggg caacggcacc cagtaagcct cgcgccaccc cctcccgcgg cggcgggagg
      181 gggaaaggcg aaagggaaga tcgtgcgttc gaccggccgc cgctttcgct ataaggtccg
      241 caccgatgcc gagccccctg cccttccgcc gccgtagagg cggcccagcc gccggaggtg
      301 acaggccgtg accggcaggg acgacgacat cgccgccgcg cttccgaagg cgccgtctcc
      361 ggcgcctgcc cggcgggcgg ccgcgatcgc cgaggcgatg cgaaggttcg acggcggctg
      421 ggaggcgcta tcgtcggtcc cgggaggccg aacctccgcc gccccggcgc cgtggcgcag
      481 ccgcttcaac cgtccccagg ccggagccct tgcaggcgcg ctcctggtcg ccctcgtcgc
      541 tctgcctctg gcctggtcct cgctccccga ccaggcgcgc gagggcggcg ggaagcggac
      601 ccagtccccc gatccggcct cgatcgcggc tccggccgcc ggtcccgctt cgccgtcggc
      661 ggatccggcg cccgacctgg cggagccggt gcttgccaat cccgcgacgc cgccggcacc
      721 ggccggcagg ccggtctcgg cttcgacgga tgagcctgcc gcagatccgc cgggcggcag
      781 gtccggaata gccgtcgccc gatcgccggc cgagcagcgg gattcggatc ttccagcgcc
      841 ggcgccggcg gcggccgacg cagaagagat cgtcgtcacc ggatcgcgga tccgaaatcc
      901 cgacctcgga tcgccccttg ccgtcacgtc gatcggcggc gaagagttcg accgggccgg
      961 caaggcggat cggcgcgggg attggaacgc ctgcacgatc gacgatccga ggcgggacct
     1021 tcgcgcctgc cgaagtgatg tggcgtcggt cgcgagaggc gcttcgggaa aggcgtcggc
     1081 tcccctcgcc gaggggctgt ccaatgcctg gaagggcgat ctggaggcgg cggtggcggc
     1141 cttcgaccgg gcgatccagc tcgcgccgcg atcggcggcg gcccacctca atcgcggcct
     1201 cgcccgccgg cggctcggcg acggcgaccg cgccctcgcc gatctcaacc gagcggtcac
     1261 ctatgcgccc cagtcggcgc gggtttatta tcaccggagc ctcctgcttc gcgagcgcgg
     1321 cgaggccagg cgcgccctgg ccgacgagcg gcgcgccgtc gagctggacg ccgactatgc
     1381 cgagctggtg agagcgcaaa ggagacgagc gcgatgatcg ctgccccgag gaggcgcttc
     1441 tggacgagcc tctatttcca ggtcctggtc gcgatcgccc ttggcgttct caccggctgg
     1501 ctcttccccg agttcgggac ttcgctgaag ccgctcggcg acgccttcat caagctcgtc
     1561 aagatgatca tcacaccggt catcttcctc accgtcacca ccggaatcgc cggaatgtcc
     1621 gacctcaagg ccttcggacg ggtcggggcg aaggccatgg cctatttcct caccttctcc
     1681 actctcgcgc tggcggtcgg gctcgtcgtc ggcaacctcg tcaggccggg cgacggagtc
     1741 aacgcggatc cggcgaccct cgaccagggg gcggtcgcga cctatgtcga caaggcgcac
     1801 gaccagtcga tcaccggctt cgtcctcgac atcatccccg acacctt
//
LOCUS       DASVQZ010000444         5619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_190628, whole genome shotgun sequence.
ACCESSION   DASVQZ010000444 DASVQZ010000000
VERSION     DASVQZ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190628"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VF547_12485"
     CDS             complement(<1..1063)
                     /locus_tag="VF547_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8263680.1"
                     /translation="MIRRLTPLLLCLLALVGAGPAAAQQSEVQTTWRLLDYVAVDYRE
                     AVRDGRVVNDGEYKEMAEFAASVRERLGALPQKPAKAKLVAGAAALQAGVARKAAPPE
                     IERQARGLAAELIAAYPVPLAPRRMPDPARGQALYAQHCASCHGASGDARTPLAASLD
                     PAPIAFNDRARARERSLFALYQVVDQGLEGTAMTSFGHLPEDDKWALALAAGRFAYPP
                     ALAEQGRRIWESDAELRKLVPDAAALTGLSEAALAARIGPARAPAVIAYLRSHPAAVT
                     QAGGSSLAVARERLRQSLAAYEAGDRKGATELALSAYLDGFEPVEPMLAARDAGLMAR
                     VERAMGELRAAIAGNRDPAE"
     gene            1176..2432
                     /locus_tag="VF547_12490"
     CDS             1176..2432
                     /locus_tag="VF547_12490"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX8263681.1"
                     /translation="MRRTTLAALALGLLAAPAPAQDVDRRVQRVLRQTPVIDGHNDLP
                     WEIRDKYDFWRKPLDLDADTSRLEARLQTDLPRMKRGGMGAQFWSVWIPNERKGDEAI
                     RVTLEQIDIVHRMVARYPERLEMASTAADIRRIEKAGRIASLIGIEGGHQIGNSPAAL
                     RQFHALGARYMTLTHSKNNDWADSATDDPVHDGLTPFGKAIVREMNRLGMIVDLSHVS
                     PKTMLDALAATRAPVIFSHSNARALMDHARNVPDDVLKLMPANGGVVMVNFYPGFLSN
                     AYRRRMAERSAEEARLANLYSGQPERRKAALDAWDTAHPPVEGTLAEVADHIEHVRRV
                     AGVDHVGIGSDFDGIDGAAPKGLEGVDAYPALLAELARRGWSDSDLAKLAGGNLLRAM
                     ERVERVAAGMKGEPPLIATIEQLDRQ"
     gene            complement(2476..2880)
                     /locus_tag="VF547_12495"
     CDS             complement(2476..2880)
                     /locus_tag="VF547_12495"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEX8263682.1"
                     /translation="MEVRRIAEIASYHAHVYFDPETQRGDAVAIREAAADRFSVRLGT
                     VWDRPVGPHSKAMYQIAFAPELFASLVPWLMLNNRGLSILIHPNTTNQKRDHLLDAVW
                     IGAPVELDGEVLPEEGEAELAGPANTSPTIAP"
     gene            complement(2884..3549)
                     /locus_tag="VF547_12500"
     CDS             complement(2884..3549)
                     /locus_tag="VF547_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEX8263683.1"
                     /translation="MPDIAPTEMTLDELRMALAPLIPGNAVFDGWSDEALGMAAAELG
                     VPAPRARLAFPGGAADMIDAWFDAIDRAMAAAFPLERIETMKIRERISSLVMFRLEVI
                     NPHKEALRRALAILALPQNLALAGRLGWRAADRMWRIAGDRATDFNHYSKRAILMGVY
                     GSTSLVYLDDASEGLAVTRAFLARRIDDVMRFEKVKAQWRGNRDRLPSLSRFLGRLRY
                     PVV"
     gene            complement(3632..4534)
                     /locus_tag="VF547_12505"
     CDS             complement(3632..4534)
                     /locus_tag="VF547_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8263684.1"
                     /translation="MQHHRSTSSAGAGRHAFAALIVGNLILALGPWMVRLADVGPVAS
                     GFWRVALAVPFLALLAWRQQSGRAPAPLGLVAMVAVGGLFFAADLAAWHVGIGLTKLA
                     NASLFGNASSFVLVLYGFVIVRRLPRKLQALALLLAAAGTALLMGSSYELSPRNFTGD
                     LFALTAGLLYTGYLIAVDRARRTMAPLPVLAIASAAATLPLLLLALALGQKVMPGDWT
                     PLLILSFGSQVVGQGLLVYAVGHLSPVVVGLGLLAQPAAGAVIGRFAYGETFSLADAI
                     GAALIAVALVLIRLPEKGPSGAGA"
     gene            4587..>5619
                     /locus_tag="VF547_12510"
     CDS             4587..>5619
                     /locus_tag="VF547_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX8263685.1"
                     /translation="MPTLFDPIQLGATLCPNRIVMAPLTRGRATREHVPTELMATYYS
                     QRAGAGLIISEATGISRQGLGWPYAPGIWNEAQRDGWKPVVEAVHEAGGRIFCQLWHM
                     GRVVHPSFLGGQPPVSASATTALYKAHTYEGRQAYVEARALELDEIKSLLDDYGRAAA
                     YAVSAGFDGVQLHAANGYLIDQFLRDGTNLRDDDYGGPIENRIRLLLEATRALADMVG
                     PERTAVRLSPNSETQGCEDSDPHALFAAAAAGLAEIGIAFLELREPGPEGTFGKSDVP
                     PVSPAIRKLFAAPLILNSDFDLARAEAALAEERADAISFGRPFISNPDLPVRLRDRAP
                     LEPDVMATWY"
BASE COUNT          891 a         1991 c         1968 g          769 t
ORIGIN      
        1 cctcggcggg gtcgcgattc ccggcgatgg cggcgcgaag ctcgcccatc gcgcgctcga
       61 ctcgcgccat cagccccgcg tcgcgagcgg cgagcatcgg ctcgaccggc tcgaagccgt
      121 cgagataggc ggacagcgcg agctcggtcg cccctttgcg gtcgccggcc tcgtaggcgg
      181 caaggctctg ccggaggcgc tcgcgcgcga cggcgaggga cgagcccccg gcctgggtga
      241 cggcggccgg atggctcctc agataggcga tcacagccgg cgcccttgcc ggcccgatcc
      301 gcgccgcgag cgccgcttcg ctcaatccgg tcagggcggc cgcgtcgggg acgagcttgc
      361 gcaattccgc atccgactcc caaatccgcc ggccctgctc tgcgagcgcg ggaggatagg
      421 cgaagcggcc cgcggccagc gcgagcgccc atttgtcgtc ctcgggcaga tggccgaagc
      481 tggtcatggc ggtgccttcg aggccctggt cgaccacctg gtagagggcg aacaggctcc
      541 gttcgcgcgc ccgcgcccgg tcgttgaagg cgatcggcgc cgggtccagg ctcgccgcga
      601 gcggcgtcct cgcgtcgccc gaggcgccgt ggcagctcgc gcaatgctgg gcatagagcg
      661 cctgcccgcg cgccgggtcg ggcatccgcc ggggcgcgag cggcaccgga taggccgcga
      721 tcagctccgc ggcgaggcct cgcgcctgcc tctcgatctc gggaggcgcc gccttgcgcg
      781 ccacccccgc ctggagcgct gccgcgcctg cgacgagctt cgccttggcc ggcttttgcg
      841 gcagggcccc cagccgctcg cgcaccgagg cggcgaattc ggccatttcc ttatattcgc
      901 cgtcgttgac gactcggccg tcgcgcaccg cctctcggta atcgacggcg acatagtcga
      961 gcagccgcca ggtcgtctgg acctctgact gttgagcggc ggccgggccg gcgccgacca
     1021 gggcgaggag acagaggaga agcggcgtca gtcggcggat catcggggca gggctaatcc
     1081 agttgcaact gattatcaat aacgacaagc tccgacgact tcggacttcc accgttgcgg
     1141 cgcccgctct aggctggccg cgaacaggag gtcccatgag aagaacgacc ttggccgcgc
     1201 tcgccctcgg gctgctcgcc gcgccggctc cggcgcagga cgtcgaccgg cgcgtgcagc
     1261 gtgtgctccg gcagacgccg gtgatcgacg gccataatga cctgccctgg gagatacgcg
     1321 acaaatatga tttctggcgc aagccgctcg acctcgacgc cgacacgtcg cgcctagagg
     1381 cgcggctgca gaccgacctg cccaggatga agcgcggtgg catgggcgct caattctggt
     1441 cggtgtggat cccgaacgag cggaagggcg acgaggcgat ccgggtgacg ctcgagcaga
     1501 tcgacatcgt ccaccggatg gtcgcgcgtt atcccgagcg gctggaaatg gcgtcaaccg
     1561 ccgccgacat ccgccgaatc gagaaagccg gacggatcgc gtcgctgatc gggatcgaag
     1621 gcgggcacca gatcggcaac tcgcccgcgg cgctccgcca gttccacgcg ctcggcgccc
     1681 gctacatgac cctgacccat tcgaagaaca acgactgggc cgattcggcc accgacgatc
     1741 cggtccacga cgggctcacc ccgttcggca aggcgatcgt gcgcgagatg aaccggctcg
     1801 gcatgatcgt cgacctcagc cacgtctccc ccaagacgat gctcgacgcc ctcgccgcaa
     1861 cccgggcgcc ggtcatcttc tcccattcca acgctcgcgc gctgatggac catgcccgca
     1921 acgtccccga cgacgtgctc aagctcatgc ccgccaacgg cggcgtcgtc atggtcaatt
     1981 tctatccggg cttcctctcc aacgcctatc gccgccggat ggcggagcgc agcgccgagg
     2041 aggcccggct cgccaacctc tattcgggcc agcccgaacg gcgcaaggct gcgctcgacg
     2101 cctgggacac ggcccatccg ccggtcgagg ggacgctggc cgaggtcgcc gaccatatcg
     2161 agcatgtccg ccgggtcgcc ggggtcgacc atgtcgggat cggctccgac ttcgacggaa
     2221 tcgacggcgc cgctccgaaa ggactcgaag gcgtcgacgc ctatcccgcg ctgctcgccg
     2281 agctcgcccg ccgcggctgg agcgattccg acctggcgaa gctggcgggg ggcaatctgc
     2341 ttcgggcgat ggagcgggtg gagcgggtcg ccgccggaat gaagggcgag ccgcccctga
     2401 tcgcgacgat cgagcaactc gaccggcaat gaaaggtcgc cggcggggac ggcgcccgct
     2461 gtcgggcccg gccgcctagg gggcgatcgt cggcgacgtg ttcgccggcc ccgcaagctc
     2521 cgcctcgccc tcctcgggca gcacctcgcc gtccagctcg accggcgctc cgatccacac
     2581 ggcatcgagc aggtggtccc gcttctgatt ggtggtgttg gggtggatga ggatgctcag
     2641 gccgcgattg ttgagcatca gccagggcac caacgatgcg aacagctcgg gggcgaaggc
     2701 gatctggtac atcgccttgc tgtgcgggcc gaccggccgg tcccacaccg tcccgagccg
     2761 aactgagaag cggtcggccg cagcctcccg gatcgccacc gcgtcgccgc gctgcgtctc
     2821 cgggtcgaaa tagacatggg cgtggtagct ggcgatctcc gcgatccgcc ggacctccat
     2881 cgctcagacc accgggtagc gcagccggcc gaggaagcgg ctgagcgaag gaagccggtc
     2941 gcgattgccg cgccactggg ccttgacctt ctcgaagcgc atgacgtcgt cgatgcgccg
     3001 cgcgaggaag gcgcgggtga cggcaagccc ctcgctggcg tcgtcaagat agacgaggct
     3061 ggtcgagccg tagacgccca tcaatatcgc gcgcttcgaa taatggttga agtcggtcgc
     3121 cctgtcgccg gcgatccgcc acatgcggtc ggccgcccgc cagccgaggc ggccggcgag
     3181 cgccaggttc tgcggcaagg cgaggatggc gagcgcccgg cgaagcgcct ccttgtgcgg
     3241 gttgatcacc tcgagccgga acatcaccag cgaggatatg cgctcgcgaa tcttcatcgt
     3301 ctcgatccgc tcgagcggga aggcggcggc catggcccgg tcgatggcgt cgaaccaggc
     3361 gtcgatcatg tcggcggcgc cgccgggaaa ggcgagccgc gcccgcgggg ccggcacgcc
     3421 cagctccgcc gcggccatgc cgagcgcctc gtcgctccag ccgtcgaaca cggcgttgcc
     3481 gggaatgagg ggggcgagcg ccattcgaag ctcgtcgagg gtcatctcgg tcggagcgat
     3541 gtcgggcatg ggcagtcaat gatcctcgaa agcgaaggat gcaagccgcg gatcgccgct
     3601 cctaccgccg accggaggcg gcggcgcgac atcaggcccc cgcgccgctc gggcccttct
     3661 ccggcagccg gatcagcacc agcgccacgg cgatcagcgc cgcgccgatc gcgtcggcga
     3721 ggctgaacgt ctcgccatag gcgaaccggc cgatcaccgc gcccgcggcc ggctgggcga
     3781 gcaggccgag gccgacgacc accggcgaga gatgaccgac cgcgtagacg aggaggccct
     3841 ggccgacgac ctggctcccg aaggagagga tgagcagggg cgtccagtcg ccgggcatca
     3901 ccttctggcc gagcgccagg gcgagcagca gcagcgggag ggtcgccgcg gcgctggcga
     3961 tggcgagcac cggcagcggc gccatcgtcc gccgcgcccg gtccacggcg atgagatagc
     4021 cggtgtagag cagcccggcg gtgagggcga agaggtcgcc ggtgaagttc cttggcgaca
     4081 gctcgtagct cgagcccatc agcagggcgg tgccggcggc ggccaggagc agcgccagcg
     4141 cctgcagctt gcgcggcagc cggcggacga tgacgaagcc gtaaagcacg agcacgaagc
     4201 tcgacgcatt gccgaacagg ctcgcattgg cgagcttggt gagtccgatc ccgacatgcc
     4261 aggcggcgag gtcggcggcg aagaacaggc ctccgaccgc gaccatcgcg accaggccca
     4321 acggcgccgg cgcccgcccc gactgctgcc gccaggcgag cagcgccagg aaggggacgg
     4381 cgagcgcgac tcgccagaag cccgacgcga ccggtccgac gtcggccagc cggaccatcc
     4441 acgggcccag cgcgaggatc agattgccca cgatcagcgc cgcgaaggcg tggcggcccg
     4501 cgccggccga ggaggttgat cgatgatgct gcattgtcgc tttcgcgccg ctaggggtcg
     4561 ccgctcctcc ccgtcaagga aagtagatgc cgaccctctt cgacccgatc cagctgggtg
     4621 cgacattatg cccgaaccga atcgtcatgg cgccgctcac ccgcggccgg gccacgcgcg
     4681 agcatgtccc caccgaactt atggcgacct attattccca gcgcgccggc gccggcctga
     4741 tcatcagcga ggcgaccgga atcagccgcc agggcctcgg ctggccctat gcgcccggca
     4801 tctggaacga ggcccagcgc gacggctgga agcccgtggt cgaggcggtg cacgaggcag
     4861 gcgggcggat cttctgccag ctctggcaca tgggacgggt ggtccacccc agcttcctcg
     4921 gcggccagcc gccggtttcg gcctcggcca ccacggctct ctacaaggcc cacacctatg
     4981 aggggcgcca ggcttatgtc gaagcgcgcg cgctggagct cgacgagatt aagagcctgc
     5041 tcgacgatta cggccgcgcc gccgcctacg ccgtctcggc cggattcgac ggggtccagc
     5101 tccacgccgc caatggctat ctgatcgacc agtttcttcg cgacggcacc aatctgcgcg
     5161 acgacgacta tggcggcccg atcgagaacc ggatccggct gctgctcgag gcgacccggg
     5221 cgctcgccga catggtcggt cccgagcgca ccgcggtgcg cctgtcgccg aacagcgaga
     5281 cccagggctg cgaggacagc gatccgcacg ccttgttcgc ggcggcggcg gccggcctcg
     5341 ccgagatcgg catcgccttt ctggagctgc gcgagccggg cccggagggc acgttcggga
     5401 agtccgacgt cccgccggta tcgccggcga tccgcaagct gtttgcagcg ccgctgatcc
     5461 tcaactccga cttcgatctc gcccgggcgg aggccgcgct cgcggaggag cgggccgacg
     5521 cgatcagctt cggccggccg ttcatctcca atcccgacct gccggtccga ctgcgcgacc
     5581 gggcgccgct cgagccggac gtcatggcga cctggtaca
//
LOCUS       DASVQZ010000445         5022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_863125, whole genome shotgun sequence.
ACCESSION   DASVQZ010000445 DASVQZ010000000
VERSION     DASVQZ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863125"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VF547_12515"
     CDS             <1..564
                     /locus_tag="VF547_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8263686.1"
                     /translation="GSDGRLVNRGFLIDSAGEIRARYDKMHLFDVDLPTGESWRESAL
                     YAPGGRAVVADSPAGRLGLSICYDLRFPDLYRALTDAGAQVLAIPAAFTVPTGRAHWH
                     VLLRARAIEAGAFVVAAAQSGRHEDGRETFGHSLVVGPWGDVRLDLGEGTGVGFAELD
                     LAEAEAVRARLPALRHRRPIPPPERAP"
     gene            561..1004
                     /locus_tag="VF547_12520"
     CDS             561..1004
                     /locus_tag="VF547_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX8263687.1"
                     /translation="MIIFDLKCGPQGHVFEAWFASSTAFAEQQARGLVACPLCGSSEV
                     AKAPMAPAVASRSNARSAATSPTSSSDDKAMLAAAADLQKRLLEGSEAVGDRFADEAR
                     AIHLGDAESRIIHGRATREEAAGLVEDGVPIAPLPFPVPSPGEEN"
     gene            1042..1118
                     /locus_tag="VF547_12525"
     tRNA            1042..1118
                     /locus_tag="VF547_12525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1076..1078,aa:Arg,seq:cct)
     gene            1141..1758
                     /gene="folE"
                     /locus_tag="VF547_12530"
     CDS             1141..1758
                     /gene="folE"
                     /locus_tag="VF547_12530"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX8263688.1"
                     /translation="MAEHRESADGDLVRSPARQPVPEEVQEAVRTLIRWAGDDPDREG
                     LLDTPARVGRAWKEYARGYEEDPSHHLSRTFEEVGGYDEIVLLKDIPFQSHCEHHMAP
                     IIGKASIAYLPAERVVGISKLARVLHGFARRLQVQERLTAQVADCICEHLRPQGVAVV
                     IEASHACMTARGVHTPGVMMTTSRMMGVFREDERSRREVLALMGY"
     gene            complement(1886..2524)
                     /gene="yihA"
                     /locus_tag="VF547_12535"
     CDS             complement(1886..2524)
                     /gene="yihA"
                     /locus_tag="VF547_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX8263689.1"
                     /translation="MSEAPPDLDEVARKLFSGPIAFLKSAPGLRFLPDPGVPEVAFAG
                     RSNVGKSSLLNALTNRNGLARTSNTPGRTQELNFFDVGSPPRFRLVDMPGYGFAKAPK
                     DVVKTWRQLINSYLRGRQVLKRALVLVDSRHGLKDIDIDVMKMLDDAAVSYHLVLTKA
                     DKVKASELAEVEAATAEQGRKHPAAHPEIIVTSAEKGMGIERLRTAVLEAVL"
     gene            complement(2521..4233)
                     /gene="yidC"
                     /locus_tag="VF547_12540"
     CDS             complement(2521..4233)
                     /gene="yidC"
                     /locus_tag="VF547_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX8263690.1"
                     /translation="MNDHRNMILAIVLSAIVLIGWSFVSEQYFPTARPPATKVVDGKS
                     VPVPQPKADPTADGPIAIRDRRQVLGETPRVPIRTPHLAGSINLKGARIDDLVLTTHR
                     ESLARNSPAIRLLSPAGSPAAYFASFGWAGRNVPGPETVWTPSATRLAPGSPVTLSWD
                     NGQGQLFLIKLAVDDNYLFSVEQRVVNRGAGDIAVRPYSVLSRIGASKDPDPWTAHAG
                     PMGVFNGAADYGNDYSDVAEAGERRFNSRGGWIGFTDTFWLAAVIPDQGRSVDASFRH
                     NRSTDSYQADAIAAPAITPRGAQSAYTSHLFAGAKEIQLLDRYTEQLGTPLERAIDWG
                     WFRWSMKPIFSLLNWLYALIGNFGVAIICLTLIVRTLMFPIAQKQFKSMAAMRVLQPK
                     MKALQDRYKDDKPRLQQEMLKLYQEEKVNPMAGCLPILIQIPIFYALYKVLMVSVEMR
                     HKPFVLWIKDLSAPDPLTPVNLFGYLPFTPPGFLALGVLPILLGITMWLQFKLNPPAT
                     DPVQRQMFAIMPWVLMFVMAPFAAGLQLYWTVNNVLTILQQKWLYSRYPGMNAPAEAA
                     APAK"
     gene            complement(4244..4456)
                     /gene="yidD"
                     /locus_tag="VF547_12545"
     CDS             complement(4244..4456)
                     /gene="yidD"
                     /locus_tag="VF547_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX8263691.1"
                     /translation="MLARALVLIARGWQLGPSRVLPPSCRYEPSCSAYAITALERYGA
                     LRGSWLAAKRLLRCHPWGGSGPDPVP"
     gene            complement(4459..4887)
                     /gene="rnpA"
                     /locus_tag="VF547_12550"
     CDS             complement(4459..4887)
                     /gene="rnpA"
                     /locus_tag="VF547_12550"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8263692.1"
                     /translation="MSALTRRADFLAANRGRRAPMPGFVLLVRPRDDGDPSIRLGITV
                     TRKIGGAVVRNRMKRRLRNIARELLPAKGLAGADHVLIGRQAGIERDFALLKGEMELA
                     LAKLAKRSVRPDPGEGPSSASSSNAKQGKASTGSARTDGG"
     gene            complement(4884..5018)
                     /gene="rpmH"
                     /locus_tag="VF547_12555"
     CDS             complement(4884..5018)
                     /gene="rpmH"
                     /locus_tag="VF547_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8263693.1"
                     /translation="MKRTFQPSNLVRKRRHGFRARTATVGGRKVLAARRARGRKTLSA
                     "
BASE COUNT          767 a         1697 c         1768 g          790 t
ORIGIN      
        1 gggagcgacg gccggctcgt aaatcgcggc ttcctgatcg attccgccgg cgagatccgc
       61 gcccgctacg acaagatgca cctgttcgac gtcgacctcc cgaccgggga gagctggcgc
      121 gaatcggcgc tctacgcgcc cggcggccgc gccgtcgtcg ccgactcgcc ggccggccgg
      181 ctcggccttt ccatctgcta cgatctgcgc ttccccgacc tctaccgggc gctgaccgac
      241 gcgggcgcgc aagtgcttgc gattccagcg gctttcacgg tgccgacagg gcgggcgcac
      301 tggcatgtgc tgcttcgcgc ccgggcgatc gaggcgggcg cgttcgtcgt cgccgcggcc
      361 cagtcgggcc gccacgagga cggccgcgag acgttcggcc acagcctcgt cgtcggcccc
      421 tggggcgacg tccggctcga cctgggggag gggacggggg tcggcttcgc cgagctcgac
      481 ctcgccgagg cggaggccgt gcgcgcccgc ttgccggccc tgcgacaccg ccgcccgatc
      541 ccgccgccgg agcgggctcc atgatcatat tcgatcttaa gtgtgggccc caaggccatg
      601 ttttcgaagc ctggtttgct tcgtcgaccg ccttcgccga gcagcaggcg agagggctgg
      661 tcgcctgtcc cctgtgcggc tcgtccgagg tcgccaaggc gccgatggcg ccggcggtgg
      721 cgtcccgctc gaatgcacgt tccgcggcaa cttcgccaac atcctcctcc gacgacaagg
      781 cgatgctcgc cgccgccgcc gatctccaga agcgcctgct cgaaggctcg gaggcggtcg
      841 gcgaccgctt cgccgacgag gcccgggcga tccacctcgg cgacgccgag agccggatca
      901 tccacggccg agcgacccgc gaggaggcgg ccgggctggt cgaggacgga gtgccgatcg
      961 cgcctctgcc cttcccggtc cccagtcccg gcgaggagaa ttgacccggg cgccgtcgcg
     1021 ccctagtccg tcgcaggcgg cggccccgta gctcagcagg atagagcatc agattcctaa
     1081 tctgagggcc acaggttcga atcctgtcgg ggtcaccacc gccgccgggc ggagaggatc
     1141 atggccgaac atcgcgaatc cgccgacgga gatctcgtcc gctcccccgc ccggcagccg
     1201 gtgcccgagg aggtgcagga ggcggtccgc accctgatcc gctgggccgg cgacgatccc
     1261 gaccgcgagg ggctgctcga caccccggcg cgcgtcggcc gggcctggaa ggaatatgcg
     1321 cgcggctacg aggaggaccc gtcccaccac ctcagccgaa ccttcgagga ggtcggcggc
     1381 tatgacgaga tcgtgctctt gaaggacatc cccttccagt cgcattgcga gcatcacatg
     1441 gcgccgatca tcggcaaggc gtcgatcgcc tatcttccgg ccgagcgagt cgtcggaatc
     1501 tccaagctcg cccgagtcct ccacggcttc gcccgccggc tccaggtcca ggagcggctg
     1561 accgcgcagg tcgccgactg catctgcgag catctgcgcc cgcagggcgt cgccgtcgtc
     1621 atcgaggcga gccacgcctg catgaccgcc cgcggggtcc acacgcccgg ggtgatgatg
     1681 acgacgagcc ggatgatggg cgtcttccgc gaggacgaac gaagccgccg cgaggtgctg
     1741 gcgctgatgg gctattgagg ccgtccttcg atacgggcct tcgccttcgc tcagtcccta
     1801 ctcaggatga gcggttggag gtgtccacca ccgctcatcc tgagtagccg gtgagcctgt
     1861 cgaaccggcg tatcgaagga cgctcctaca gcaccgcctc cagcaccgcc gtcctcagcc
     1921 gctcgatccc catgcccttc tcggccgagg tgacgatgat ctccgggtgg gcggcgggat
     1981 gcttccggcc ttgctcggcg gtcgcggcct cgacctcggc cagctccgag gccttcacct
     2041 tgtcggcctt ggtgaggacg aggtggtagc tgacggccgc gtcgtcgagc atcttcatga
     2101 cgtcgatgtc gatgtccttg agaccgtgcc tcgaatcgac gaggacgagc gcgcgcttca
     2161 ggacctgccg gccgcgcagg taggaattga tcagctggcg ccacgtcttg acgacgtcct
     2221 tgggcgcctt ggcgaagccg tagccgggca tgtcgaccag ccggaagcgc ggcggcgagc
     2281 cgacgtcgaa gaaattgagc tcctgggtcc ggcccggcgt gttcgaggtg cgggcgaggc
     2341 cgttgcggtt ggtgagggcg ttgagaagcg acgacttgcc gacgttggag cggcccgcga
     2401 aggccacttc gggaacgccg ggatcgggca ggaacctgag gcccggcgcc gacttcagga
     2461 aggcgatcgg gcccgagaac agcttccggg cgacctcgtc cagatccggc ggagcctcgc
     2521 tcacttggcc ggcgcggccg cttcggccgg cgcgttcatc cccggatagc ggctgtagag
     2581 ccacttctgc tggaggatgg tgaggacgtt gttgaccgtc cagtagagct ggagccccgc
     2641 cgcgaacggc gccatcacga acatcagcac ccagggcatg atcgcgaaca tctgcctctg
     2701 caccggatcg gtggccggcg ggttgagctt gaactggagc cacatcgtga tcccgagcag
     2761 gatcggaagc acgcccagtg ccaggaaccc aggcggcgtg aagggcaggt agccgaacag
     2821 gttgaccggg gtcagcgggt cgggggcgga gaggtccttg atccacagca cgaacggctt
     2881 gtggcgcatc tccaccgaaa ccatcagcac cttgtagagc gcgtagaaga tcgggatctg
     2941 gatgaggatg ggcaggcacc cggccatcgg gttgaccttc tcctcctggt agagcttcag
     3001 catctcctgc tggaggcgcg gcttgtcgtc cttgtagcgg tcctgaagcg ccttcatctt
     3061 cggctggagg acccgcatcg ccgccatcga cttgaactgc ttctgggcga tcgggaacat
     3121 cagcgtcctc acgatcaggg tgaggcagat gatcgccact ccgaaattgc cgatcagcgc
     3181 gtagagccag ttcagaagcg agaagatcgg cttcatggac cagcggaacc agccccagtc
     3241 gatcgcccgc tcgagcggcg tgccgagctg ctcggtatag cggtccagga gctggatctc
     3301 cttcgcgccg gcgaagagat gggaggtgta ggccgactga gcgccgcgcg gggtgatcgc
     3361 cggcgccgcg atcgcgtcgg cctggtagct gtcggtggag cggttgtggc ggaacgaggc
     3421 gtcgaccgat ctgccctggt ccgggatcac cgccgccagc cagaaggtgt cggtgaagcc
     3481 gatccagccg ccgcggctgt tgaagcgccg ctcgcccgcc tcggcgacgt cgctatagtc
     3541 gttgccgtaa tccgccgcgc cgttgaacac gcccatcggt ccggcatggg ccgtccacgg
     3601 atcgggatcc ttcgacgcgc cgatccggct cagcaccgaa taggggcgca cggcgatgtc
     3661 gccggcgccg cgattgacca ctctctgctc caccgagaac aggtaattgt cgtcgaccgc
     3721 cagcttgatc aggaacagct ggccctggcc gttgtcccag ctcagggtga ccgggctgcc
     3781 cggcgccagc cgggtcgcgc tcggcgtcca gaccgtctcc gggcccggca cgttccggcc
     3841 cgcccagccg aagctggcga aataggcggc ggggcttccg gcgggcgaga gcaggcggat
     3901 cgccggcgag ttgcgggcca ggctctcgcg atgggtggtg agcacgagat cgtcgatgcg
     3961 cgcgcccttg aggttgatcg atccggccag atgcggcgtc cggatcggca cccgaggggt
     4021 ctcgccgagc acctggcggc ggtcgcggat ggcgatcggg ccgtcggcgg tcgggtcggc
     4081 cttgggctgc ggcaccggca ccgacttgcc gtcgacgacc ttggtcgcgg gggggcgggc
     4141 ggtggggaaa tactgctcgc tgacgaagct ccagccgatg agcacgatcg ccgacaggac
     4201 gatcgccagg atcatgttgc ggtggtcgtt cacctatttt ccctcacggt accggatcgg
     4261 ggcccgagcc cccccagggg tggcatctga gcagacgttt ggcggccagc caggagccgc
     4321 gcagcgcccc atagcgctca agcgcggtga ttgcataggc cgagcagctg ggctcgtagc
     4381 ggcagctcgg cggcagcacc cgggaaggcc cgagctgcca cccgcgcgcg atcaggacga
     4441 gcgcacgagc gagcaacctc atcccccatc cgttcgcgct gagcctgtcg aagccttccc
     4501 ttgctttgca ttcgaggagg aagcggagga tgggccttcg ccaggatcag gccgaaccga
     4561 gcgtttggcc aatttggcga gcgcaagctc catctcgccc ttcagcaagg cgaaatcgcg
     4621 ctcgatcccg gcctggcggc caatcagcac atggtccgcg ccggccaggc ccttcgcggg
     4681 cagcagttcg cgggcgatgt tccgcaagcg gcgcttcatc cggttgcgga ccacggcgcc
     4741 gccgatcttc cttgtgacgg tgattccgag ccggatcgac gggtcgccgt cgtccctcgg
     4801 ccggacgagc aggacgaagc cgggcatggg cgctcggcgg ccgcgattgg cggcgaggaa
     4861 atcggcccgg cgggtgaggg cgctcaggcg gagagggtct tgcggccccg cgcccggcgc
     4921 gcggccagaa ccttgcggcc gccgacggtc gccgtccggg cccggaagcc gtgccggcgc
     4981 ttgcgcacga ggttgctcgg ctggaaggtg cgcttcatcg ct
//
LOCUS       DASVQZ010000446         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_956797, whole genome shotgun sequence.
ACCESSION   DASVQZ010000446 DASVQZ010000000
VERSION     DASVQZ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956797"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VF547_12560"
     CDS             <1..556
                     /locus_tag="VF547_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8263694.1"
                     /translation="EGRHASGISIEVEGAAAQLNQKPITGAVLAGLMKVYSDSVNMVN
                     APFLAKERGLDVREVRHDREGDYHTLVRVSVATPDGPHAVEGTLFGNAAPRLVGMFGI
                     RVESELEGEMIFIVNEDTPGFIGRLGTLLGEAGVNIGTFSLGRREAGGEAIALVSVDS
                     HIGEGLVEALQGLPGVKRVEPLRF"
     gene            737..2026
                     /locus_tag="VF547_12565"
     CDS             737..2026
                     /locus_tag="VF547_12565"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX8263695.1"
                     /translation="MANVTVIGVQWGDEGKGKIVDWLASRADVVARFQGGHNAGHTLV
                     VGEQVYKLSLLPSGIVRGTLSVIGNGVVLDPWALKAEIERLAEQGVAVTAETLHIADT
                     CPLILPFHRDLDALREDASGQGKIGTTRRGIGPAYEDKVGRRAIRVCDLAHLGHLGPQ
                     IDRLCAHHDALRAGFGEPPIDRVRLERDLGEIAEFVLRFARPVWKTLNEARARGKRIL
                     FEGAQGVLLDVDHGTYPFVTSSNTIAGTAAGGSGLGPAAAGFVLGIVKAYTTRVGAGP
                     FPTELDDATGQRLGERGREFGTVTGRKRRCGWFDAVLVRQACAVAGVTGVALTKLDVL
                     DGLDEIRICTGYRLRGKAFDHLPAHAADQAAVEPVYETMKGWTESTAGARSWADLPAQ
                     AIKYIRRIEELCRISVALVSTSPEREDTILVRDPFAD"
     gene            2097..>2403
                     /locus_tag="VF547_12570"
     CDS             2097..>2403
                     /locus_tag="VF547_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8263696.1"
                     /translation="MTQLNILVTGSSRGIGKAIVAALAGHKVASHSSRGGDGLIAADL
                     AEPGAAEALWNEALERLGGRIHILVNNAGIFEAAPVDLPAGEWTAQWQRTMRVNLTAS
                     "
BASE COUNT          367 a          850 c          847 g          339 t
ORIGIN      
        1 cgaagggcgc cacgccagcg gcatctcgat cgaggtggaa ggcgccgcgg cccagctcaa
       61 ccagaagccg atcaccggcg ccgtcctggc cgggctgatg aaggtctatt cggacagcgt
      121 gaacatggtc aacgcgccct tcctggcgaa ggagcgcggg ctcgacgtcc gcgaggtgcg
      181 gcacgaccgc gagggcgact atcacactct ggtgcgggtc agcgtcgcca cgcccgacgg
      241 accgcacgcg gtcgagggca ccttgttcgg caatgccgca ccgcgcctgg tcggaatgtt
      301 cggaatccgg gtcgagtcgg agctcgaggg cgagatgatc ttcatcgtca acgaggacac
      361 gccgggcttc atcggccggc tcggcaccct gctcggcgag gccggggtca acatcggcac
      421 gttcagcctc ggccgccgcg aggcgggcgg cgaggcgatc gcgctggtct cggtcgacag
      481 ccatatcggc gaggggctgg tcgaggcgct tcagggcctg ccgggggtga aaagggtcga
      541 gccgctgcgc ttttgatttc cggtccgtcc cgttaaagcg gcggtggttc gggtccagcg
      601 ccgttcgggc tgagcttgtc gaagccctct tcttcccctg aaggcaggaa ggaagaccct
      661 tcgacaggct ctgggtgaac ggaggccagg agttccgacc tgctgaattc caagggcacc
      721 agcacaggag ccgcaattgg ccaacgtcac cgtcatcgga gtccagtggg gcgacgaggg
      781 gaagggcaag atcgtcgact ggctggcgag ccgggccgac gtcgtcgccc gcttccaggg
      841 cggccataac gcgggccaca cgctggtcgt cggcgagcag gtctacaagc tgtcgcttct
      901 tccctccggg atcgtccggg gcaccctctc ggtcatcggc aacggcgtcg tcctcgatcc
      961 ctgggcgctg aaggccgaga tcgagcggct cgccgagcag ggcgtggcgg tgaccgccga
     1021 gaccctgcac atcgccgaca cctgcccgct catcctcccc ttccaccgcg atctcgacgc
     1081 gctcagggag gacgcgtccg gccagggcaa gatcggcacc acccgccggg gcatcggccc
     1141 ggcctatgag gacaaggtcg gacgccgcgc gatccgggtc tgcgacctcg cccatctcgg
     1201 ccatctcggc ccgcagatcg accgcctctg cgcccatcac gacgcccttc gcgccggctt
     1261 cggcgagccg ccgatcgacc gcgtccggct cgagcgcgac ctcggcgaga tcgccgagtt
     1321 cgtgcttcgc ttcgcgcgcc cggtctggaa gacgctcaac gaggcgcggg cgcggggcaa
     1381 gcggatcctg ttcgaaggcg cgcagggcgt tctgctcgac gtcgaccacg gcacctatcc
     1441 gttcgtcacc tcgtcgaaca cgatcgccgg caccgcggcc ggcggcagcg gcctcgggcc
     1501 ggccgcggcg ggcttcgtgc tgggcatcgt caaggcctac accacccggg tcggcgcggg
     1561 ccccttcccg accgagctgg acgacgccac cggccagcgc ctcggcgagc gcggacgcga
     1621 gttcggcacc gtgacgggac gcaagcggcg ctgcggctgg ttcgacgcgg tgctcgtccg
     1681 ccaggcctgc gcggtggccg gcgtgaccgg cgtcgcgctg accaaattgg acgttctcga
     1741 cggcctcgac gagatccgga tctgcaccgg ctaccggctg cgcggcaagg cgttcgacca
     1801 tctgcccgcc cacgccgccg accaggctgc cgtcgagccg gtctacgaga cgatgaaggg
     1861 atggacggaa tcgaccgccg gagcgcgcag ctgggccgac ctgccggccc aggcgatcaa
     1921 gtacatccgc cggatcgagg agctgtgccg gatctcggtc gcgctcgtct cgaccagccc
     1981 ggagcgcgag gacacgatcc tggtgcgcga cccgttcgcc gactgacggc ctccgatgcg
     2041 cggcctgggg aggcaaagag cttcccctgc gctgcgtcgg cgactatcgc gccgccatga
     2101 cccagctcaa catcctcgtc accggcagca gccgcggaat cggcaaggcg atcgtcgccg
     2161 cgctcgccgg ccacaaggtc gccagccatt cgagccgcgg cggcgacggc ctgatcgccg
     2221 ccgacctcgc cgagccgggg gcggccgagg cgctctggaa cgaagcgctc gagcggctcg
     2281 ggggccggat ccacatcctg gtcaacaatg ccgggatctt cgaggcggcg ccggtcgacc
     2341 tgccggccgg cgagtggacc gcgcagtggc agcggacgat gcgggtcaac ctcaccgcct
     2401 ccg
//
LOCUS       DASVQZ010000447         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_479417, whole genome shotgun sequence.
ACCESSION   DASVQZ010000447 DASVQZ010000000
VERSION     DASVQZ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479417"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..494)
                     /locus_tag="VF547_12575"
     CDS             complement(69..494)
                     /locus_tag="VF547_12575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8263697.1"
                     /translation="MSMTLAAALLAAAAAPAPEPAPGTKLIVDTGTTMAGQPLAVPAS
                     PFRVVATRVELPAGSAVPTHMHIWARYVYVERGEVQLTLADSGTSRTFRAGELIVEPI
                     GKWHSGRIVSDTILVAVEQVPTARCNTVKPPEPGKSNDC"
     gene            complement(553..876)
                     /locus_tag="VF547_12580"
     CDS             complement(553..876)
                     /locus_tag="VF547_12580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8263698.1"
                     /translation="MSFTAELSTSPDHDGRRAARAEVVLGAGLRQRGAHAVTVQVVDL
                     SITGFRAATHLELMPGADIWLKLPGLESLHSRVVWMKGHWLGCEFVRPLHPAVLDMIV
                     RNSAR"
     gene            complement(886..1317)
                     /locus_tag="VF547_12585"
     CDS             complement(886..1317)
                     /locus_tag="VF547_12585"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8263699.1"
                     /translation="MGVEHFVDGPVAGKRKAKRARVLLAARLQTASGEIEARLRDLSR
                     KGALVECQTVPPVGTEVVFSRGNTSIPARIAWTGPDRVGLEFAYMIDEHEVLVQLKRT
                     SGDQIQPRFRRPRLFGEAMSDQEKQLARLWGVSVGIAIPGE"
     gene            1488..>1974
                     /locus_tag="VF547_12590"
     CDS             1488..>1974
                     /locus_tag="VF547_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263700.1"
                     /translation="MRIVKWLIGTAVAASVAASAASAAGPEPSVAAFEAAMLDLDYGR
                     ASEIADALASRHRLAQKETRPDALLSGLFGRLFLKRGLPAVALPYLQHADAPDLPAPQ
                     RLAAGFARAEAEEALGQWAAAAATLERLLPLPLDAEQRFEARLGLARVRLADDPRAAF
                     AE"
BASE COUNT          285 a          699 c          701 g          289 t
ORIGIN      
        1 tggtaaggct gaggtcgtga gttcaatcct caccggcagc accattcgcc gtcgcggccc
       61 gtcccgggtc agcagtcgtt ggacttgccc ggctcgggcg gcttcaccgt gttgcagcgg
      121 gcggtgggca cctgctcgac cgcgaccagg atcgtgtcgc tgacgatccg gcccgaatgc
      181 catttgccga tcggctcgac gatcagctcg ccggccctga aggtccggct cgtccccgaa
      241 tcggcgagcg tcagctgaac ctcgccgcgc tcgacataga cgtagcgggc ccagatatgc
      301 atgtgggtcg gcaccgccga gccggccggc agctcgaccc gagtcgccac cacccggaag
      361 ggcgaggccg gcaccgccag cggctggccg gccatggtcg tgccggtgtc gacgatgagc
      421 ttggtcccgg gcgccggctc gggcgcgggc gccgcggctg cggcgaggag cgcggcggcg
      481 agcgtcatcg acatcgatct tccccttgct ggaccggcgc aggatgcgcc gatccgaggc
      541 gcgggtccac cgtcagcggg cggagttgcg gacgatcatg tcgagcacgg cgggatggag
      601 cggccggacg aactcgcagc cgagccaatg gcccttcatc cacacgaccc gagaatggag
      661 cgattcgagg cccggcagct tcagccagat gtcggcgccg ggcatcagct cgagatgggt
      721 ggcggcccgg aagccggtga tggagagatc gaccacctgg accgtgaccg catgcgcgcc
      781 gcgctgccgc aggcccgcgc cgagcaccac ttcggcccgg gcggcgcggc ggccgtcatg
      841 gtcggggctt gtggagagct cggcggtgaa gctcatggac aggcctcact cccccggaat
      901 ggcgatgccg accgagacgc cccagaggcg ggccagctgc ttctcctggt cgctcatcgc
      961 ctcgccgaac agccgcggac ggcggaagcg gggctggatc tggtcgcccg aggtgcgctt
     1021 cagctgcacc agcacctcgt gctcgtcgat catgtaggcg aattcgaggc cgacacggtc
     1081 cggaccggtc caggcgatgc gggcgggaat gctggtgttg ccgcggctga acacgacctc
     1141 ggtgccgacc ggcggcaccg tctggcactc gaccagcgcg cccttgcgcg agaggtcgcg
     1201 cagccgcgcc tcgatctcgc cggaggccgt ctgcagcctt gcggcgagca gcacccgcgc
     1261 ccgcttcgcc ttgcgcttgc cagccaccgg tccgtcgacg aaatgctcta cgcccatcgc
     1321 gttcttttcc tcattggatg tcggcgaaga gcgataggcc gaaggcacct aaattgtagt
     1381 aaacaagggc gccattttct gctccgcgcg aagccgccgc ggccgccaga atccgctggc
     1441 tcggcggcgc tttagcggca atgttggagg caaggcgcgg ggagcggatg agaatcgtca
     1501 aatggttgat cgggactgcg gtcgccgcct cggtcgcggc ttcggccgcg tccgcagccg
     1561 ggccggaacc gtccgtcgcg gcgttcgaag ccgcgatgct cgacctcgac tacggacgag
     1621 cgtcggagat cgcggacgcc ctggcgagcc gccatcggct cgcccagaag gaaacccggc
     1681 ccgacgcgct gctgagcggg ctcttcggcc ggctgttcct gaagcgcggc ctgcccgccg
     1741 tcgccctgcc ctatctgcaa catgcggatg cgcccgatct gcccgcgccc cagcgcctgg
     1801 cggccggctt cgcgcgcgcc gaagccgaag aagcgctcgg ccagtgggcc gccgccgccg
     1861 ccaccctgga gcgcctgctg ccgctgccgc tcgacgccga gcagcggttc gaggcccgcc
     1921 tcggccttgc ccgagtccgg ctcgccgacg atccgcgcgc ggcgttcgcc gagg
//
LOCUS       DASVQZ010000448         6046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_91696, whole genome shotgun sequence.
ACCESSION   DASVQZ010000448 DASVQZ010000000
VERSION     DASVQZ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91696"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VF547_12595"
     CDS             complement(<1..1495)
                     /locus_tag="VF547_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8263701.1"
                     /translation="MTNRFDSRRSGLLGGVAAFALALGSASPAAAQDVPDTEETSEAA
                     TPSEGTEADVPGTEQDIVVTGFRASLENAVNEKKQQDQIIESVSAEDIGKLPDASIAE
                     SIARLPGLTSQRLSGRANVISIRGFGPDFSMTLLNGREQTSTGDNRAVEFDQYPSEVV
                     RQVNVYKSPSASLVGQGLVGTVDIRTIRPLDFGGRQVLAVGARGSYADLGKLNAGSND
                     KGFRVNATFVDQFADDTVGLAMAASYVDEPYQLQEYNAWGYPGSGTAADPYVIGGSKS
                     YVTSTQLKRLGLQGTMQFRPTRTFTATIDAFYSNFQDDQIKRGIELPLAWGNASLQPG
                     SGKVENGFLVSGIATGVEGVIRNDAFERHAKLFSTGINLNYEGENGWNGFFDFGYSRT
                     DRNELILESYSGTGFGANNGARDTIAFQATERGIIFDPTLNYSDTNLIKLTDPQGWGG
                     GVLPQVGYYNNRVVDDELMQFRGQVERELEGGFLSAIKAGANYTLREK"
     gene            1918..3720
                     /locus_tag="VF547_12600"
     CDS             1918..3720
                     /locus_tag="VF547_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8263702.1"
                     /translation="MRPISTLAALALAAVPVPAAAETYLDRSPADEIVYFVLPDRFEN
                     GDPSNDRGGVAGDRLRTGFDPAAKGFYHGGDLKGLMRRLDYIAGLGATAIRLGPIYRN
                     KPVQGPPGRESAGYHGYWITDFTDVDPHFGSREDLKRLVDAAHARGMKVYLDIITNHS
                     ADVIKYRECPANDCAYRSLADYPHSRRGGPAGETINPGFEGIDKPAQRPDNFARLTRP
                     DFAYTPWVPPAEAGVKKPAWLNDPIYYHNRGDTHWTGESATLGDFSGLDDLFTEHPRV
                     LAGFIEIFGQWIDDFGIDGCRIDTARHVNPEFWQAFVPAMLERARARGIPNFHIFGEV
                     YDPDPGTLARFTRVDSYPTVLDFAFQSAVTDVVAKGQGGARLARLFAADALYAGGEAT
                     ARQLPTFLGNHDMGRFARFVREGHPQASDDEVLKRVVLGHAIMFLSRGVPVVYYGDEQ
                     GFAGDGGDQDAREDMFPSKVAVYNDNRLVGSSATTAQSNFDPQSKLYRAIAALAALRR
                     ADPALRHGEQTVRAAGEAPGLLALSRRAPSGGGETLVLFNSSTAPAAANVVVEPGSER
                     WTSVRGRCAARSAAPGSYRVEVPALDYVVCRTAR"
     gene            3717..5336
                     /locus_tag="VF547_12605"
     CDS             3717..5336
                     /locus_tag="VF547_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8263703.1"
                     /translation="MNQIALDRFGAAAAADGDEWWRGAVIYQIYPRSFADSNGDGIGD
                     LPGIIERLGHVASLGVDGIWLSPFFTSPMKDFGYDIADYRDVDPVFGTLADFDRLLDR
                     AHALGLKVLIDQVYSHSSDRHAWFQESRSSRDNAKADWYVWADARPDGSPPNNWQSVF
                     HGPSWTWDARRGQYYLHNFLAGQPDLNVHKREVQDALIDVARYWLDRGVDGFRLDAIN
                     FAMHDPLLRDNPPVAEGLGRRTRPFDFQHHFHNQSHPAIPDFLGRLRAAVDGYGAAFT
                     VAEVGGEQADREMKLYTKGRNRLNSAYGFNFLYARTLSPDLIDSAMRLWPGEPDEGWP
                     SWAFSNHDAPRVVSRWLGGRDAGAFARQAMLLLMCLRGNVFLYQGEELGLPQADVPFE
                     RLSDPEAIANWPETQGRDGARTPMPWLSGAANAGFSAGEPWLPVDPAHVPLAVDAQDG
                     DPDSVLNLTRRLVALRRSKPALRTGAIRRVDAPAPLLVFERGEGADALLCAFNLGEES
                     VDWAPPGGWRIVERVGEPMAPLSGLVAERAG"
     gene            complement(5468..>6046)
                     /locus_tag="VF547_12610"
     CDS             complement(5468..>6046)
                     /locus_tag="VF547_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8263704.1"
                     /translation="SDARFAVWGAQLPDQSYCSVGSDNLAGGRRATAHLMRLSRHRIV
                     FLGDTEAPEVAQRVRGYVEAHEQLGWPVDPELMVPAHFTVESAEASVDTLLARGIPFN
                     GIVAASDLIALGAIRSLNHHNIKVPRDVSVVGYDDVPFARYSRPALTTVKQDTARAGR
                     LLVSKLLDAGAATDMRSERLPTDLIVRESCGG"
BASE COUNT          899 a         2086 c         2086 g          975 t
ORIGIN      
        1 tcttctcgcg aagggtgtaa ttggcgcctg ccttgatcgc ggaaaggaag ccgccttcga
       61 gctcgcgctc gacctgcccg cggaactgca tcagctcgtc gtcgacgacg cggttgttgt
      121 aatatccgac ctgcggcagg actccgccgc cccagccctg cgggtcggtc agcttgatca
      181 ggttcgtgtc ggaatagttc agggtcggat cgaagatgat gccgcgctcg gtcgcctgga
      241 aggcgatcgt gtcgcgggcg ccgttattcg cgccgaagcc ggtgccggaa tagctttcga
      301 ggatcagctc gttgcggtcg gtgcgcgaat agccgaagtc gaagaagccg ttccagccgt
      361 tctccccctc gtagttgagg ttgattccgg tcgagaagag cttggcatgg cgctcgaagg
      421 cgtcgttgcg gatcacccct tcgacgccgg tggcgatgcc ggagacgagg aagccgttct
      481 cgaccttgcc cgaccccggc tgcaggctgg cattgcccca ggccagcgga agctcgatgc
      541 cgcgcttgat ctgatcgtcc tggaagttcg agtagaaggc gtcgatcgtc gccgtgaagg
      601 tgcgggtcgg ccggaactgc atcgttccct gaaggccgag gcgcttgagc tgggtcgagg
      661 tgacgtagga cttcgagccg ccgatcacat agggatccgc cgcggtgccg ctgcccggat
      721 agccccaggc gttatattcc tgcagctggt agggctcgtc gacgtaggag gcggccatgg
      781 cgaggccgac cgtgtcgtcc gcgaactggt cgacgaaggt ggcgttgacc cggaagccct
      841 tgtcgttcga gcccgcgttg agcttgccca gatcggcgta ggagcctcgg gcgccgacgg
      901 cgagcacctg gcgaccgccg aaatcgagcg gccggatggt gcggatgtcc acggttccga
      961 ccaggccctg gccgaccagg ctcgcgctcg gcgacttgta gacgttgacc tgacggacga
     1021 cctcggaagg atattggtcg aattcgacgg cgcggttgtc gccagtcgag gtctgctcgc
     1081 ggccgttgag gagggtcatc gagaaatcgg gcccgaagcc gcggatcgag atgacgttgg
     1141 cgcggccgga cagacgctgc gaggtgaggc ccgggaggcg agcgatcgat tcggcgatcg
     1201 aggcgtcggg aagcttgccg atgtcctcgg ccgagaccga ctcgatgatc tggtcctgct
     1261 gcttcttctc gttgaccgcg ttctcgagcg aggcccggaa gccggtgacg acgatgtcct
     1321 gctcggtccc cgggacgtcc gcctcggtcc cttcgctcgg cgtcgcggcc tcgctggtct
     1381 cctcggtgtc cggcacgtcc tgcgccgcgg ccgggctcgc cgagcccagc gctagcgcga
     1441 aggcggcgac gccgccgagc agtccggagc ggcgcgagtc gaagcgattc gtcattctcg
     1501 ggtcctctca gttcagccgc cgaatgcccc cacccggacg acccgttgca cccggaggag
     1561 ccctgcgcgc gtcgccgaaa aaacggcgga aaagcgcgta tccgcacccc ctgtttccct
     1621 cggcgccggc ctcggccgga cgacttccgc cggacgccgt tcgccgcgat tggctcgcgg
     1681 tctcgtggct ttcgcaaatt actgcaaact aatggccccc ttggcaagag cgaaattttc
     1741 gcgggctccg aggctgtggc tcatcggcca cggcgcgagg ttgagtttgc tcggttttct
     1801 cccctttccg gcccgtgagg ggcggccctg cgccgctggc cggggctctt gaccgccgcg
     1861 ccggggttct gcaaagaatt gcaaaaagct gctgaggcgg cggagaggag caggccgatg
     1921 cggccgattt caacccttgc ggcgctggcc ttggcggcgg tcccggtccc ggcggcggcg
     1981 gaaacctatc tcgaccggtc gccggccgac gagatcgtct atttcgtgct tccggaccgg
     2041 ttcgagaacg gcgatccgtc caacgaccgg ggcggcgtcg ccggcgaccg gctgaggacc
     2101 ggcttcgatc cggccgccaa gggcttctac catggcggcg acctcaaggg ccttatgcgc
     2161 cggctcgact atatcgccgg cctcggcgcc accgccatca ggctcggccc gatctacagg
     2221 aacaagccgg tgcagggtcc tccgggtcgc gaatcagccg gctatcacgg ctactggatc
     2281 accgacttca cggacgtcga cccgcatttc ggctcccgcg aggacctcaa gcgcctggtc
     2341 gatgcggccc atgcccgggg gatgaaggtc tatctcgaca tcatcaccaa ccacagcgcc
     2401 gacgtgatca aatatcgcga atgcccggcc aacgactgcg cctacaggag cctcgccgac
     2461 tatccgcatt cgcgccgcgg cggccccgcc ggcgagacga tcaaccccgg cttcgagggg
     2521 atcgacaagc ccgcccagcg gcccgacaat ttcgcccggc tgacccggcc cgatttcgcc
     2581 tacacgccct gggtgccgcc ggccgaagcg ggggtgaaga agccggcctg gctcaacgat
     2641 ccgatctact accacaatcg cggcgacacg cactggaccg gcgagagcgc gacgctgggc
     2701 gatttctccg ggctggacga cctgttcacc gagcatccgc gggtcctcgc cgggttcatc
     2761 gagatcttcg gccagtggat cgacgatttc gggatcgacg gctgccggat cgacaccgcc
     2821 aggcacgtca atcccgaatt ctggcaggcc ttcgtcccgg ccatgctcga gcgggcgagg
     2881 gctcggggca ttccgaactt tcacattttc ggcgaggtct acgatcccga cccgggcacc
     2941 ctggcgcggt tcacccgggt cgattcctat ccgaccgtgc tcgacttcgc cttccagtcg
     3001 gccgtgaccg acgtcgtcgc gaagggccag ggcggcgccc ggctggcgcg gctgttcgcg
     3061 gccgacgccc tctatgccgg cggcgaagcg acggcgcggc agctgcccac cttcctcggc
     3121 aatcacgaca tgggccggtt cgcccgcttc gtccgcgaag gccacccgca ggcgagcgac
     3181 gacgaggtcc tgaagcgggt cgtcctcggc cacgcgataa tgttcctctc gcgaggcgtg
     3241 ccggtcgtct attatggcga cgagcagggc ttcgccggcg acggcggcga ccaggacgcg
     3301 cgcgaggaca tgttcccgag caaggtcgcc gtctacaacg acaacaggct ggtcggatcc
     3361 tcggccacga ccgcccagtc caacttcgat ccgcagtcga agctctaccg ggcgatcgcc
     3421 gcgctggccg cgcttcgccg ggccgatccg gcgctccgcc acggcgagca gacggtgcgc
     3481 gccgccggcg aggcgccggg cctgctcgcg ctgtcgcgcc gcgccccgtc cggcggcggc
     3541 gagacgctgg tcctgttcaa cagctcgacc gcgccggccg ccgccaacgt cgtcgtcgag
     3601 ccgggctcgg agcgctggac ctccgtccgc gggcgctgcg ccgccaggtc ggcggccccg
     3661 ggcagctacc gggtcgaggt gcccgcgctc gactatgtgg tctgcaggac ggcgcggtga
     3721 accagatcgc gctcgaccgc ttcggcgccg ccgcggcggc ggacggagac gaatggtggc
     3781 gcggggccgt catctaccag atctatccgc gcagcttcgc ggattcgaac ggcgacggga
     3841 tcggcgacct gcccggaatc atcgagcggc tcggccatgt cgcgtcgctc ggcgtcgacg
     3901 ggatctggct ctcccccttc ttcacctcgc cgatgaagga tttcggctac gacatcgccg
     3961 attatcgcga cgtcgacccg gtgttcggga cgctcgccga tttcgaccgg ctgctcgacc
     4021 gagcccacgc gctcggcctc aaggtgctga tcgaccaggt ctattcgcac agctccgacc
     4081 gccacgcctg gttccaggag agccgctcca gccgggacaa tgccaaggcg gactggtacg
     4141 tctgggccga cgcccggccg gacggctcgc cgcccaacaa ctggcaatcg gtgttccacg
     4201 gcccgagctg gacctgggac gcgcgccgcg gccaatatta tctgcacaac ttcctcgccg
     4261 gccagcccga cctcaacgtc cacaagcggg aggtccagga cgcgctgatc gacgtcgccc
     4321 gctactggct cgaccgcggc gtcgacggct tccggctcga cgcgatcaat ttcgcgatgc
     4381 acgaccccct gctgcgcgac aatccgccgg tggccgaggg ccttggccgg cgcacgcgcc
     4441 cgttcgactt ccagcaccat ttccacaacc agtcgcatcc ggccatcccg gatttcctcg
     4501 gccggctccg ggcggcggtg gacggctatg gcgcggcctt caccgtcgcc gaggtcggcg
     4561 gcgagcaggc cgaccgggag atgaagctct acaccaaggg ccgcaaccgg ctgaacagcg
     4621 cctacggctt caacttcctc tacgcccgga ccctgagccc cgacctcatc gactcggcga
     4681 tgcgcctgtg gccgggcgag ccggacgagg gctggccgag ctgggccttc tccaaccacg
     4741 acgcgccgcg ggtggtctcg cgctggctcg gcggccgcga cgccggggcc ttcgcccgcc
     4801 aggcgatgct tctgctcatg tgcctgcggg gcaatgtctt cctctaccag ggcgaggagc
     4861 tcggactgcc gcaggcggac gttccgttcg agcggctgag cgaccccgaa gccatcgcca
     4921 actggcccga gacccaggga cgcgacggcg cccggacgcc gatgccctgg ctgagcgggg
     4981 cggccaatgc gggcttctcg gccggggagc cgtggctgcc ggtcgatccg gcccatgttc
     5041 cgcttgcggt cgatgcgcag gacggggatc cggattcggt cctgaacctc actcgccgcc
     5101 tggttgcgct tcgccggtcg aagccggcgc tccgcaccgg cgcgatccgc cgcgtcgacg
     5161 cgccggcgcc tttgctggtc ttcgagcggg gcgaaggggc ggacgcgctg ctgtgcgcgt
     5221 tcaacctcgg cgaggagtcg gtggactggg cacctcccgg cggctggcgg atcgtcgagc
     5281 gggtcggcga gccgatggcg ccgctttcgg ggctcgtcgc cgagcgggcc ggatgaaatt
     5341 cctccccgag atttctcggg gagggggacc gcgcgaagcg cggtggaggg gttcttcggc
     5401 ggcaagaacc cctccaccag gcttcgcctg gtccccctcc ccggcaaatg ccgggaagga
     5461 attctcgtca gcccccgcag ctttcccgga cgatgaggtc ggtcgggagc cgctccgagc
     5521 gcatgtccgt cgccgcgccg gcgtcgagca gcttggagac gagcagccgg ccggcccgcg
     5581 ccgtgtcctg cttgaccgtg gtcagggcgg gccggctgta gcgggcgaag ggcacgtcgt
     5641 catagccgac caccgagacg tcgcgcggca ccttgatgtt gtgatggttg agcgagcgga
     5701 tcgcgccgag ggcgatgaga tcgctcgccg cgacgatgcc gttgaacggg attccgcggg
     5761 cgagcagcgt gtcgaccgac gcctcggcgg attcgacggt gaaatgcgcc ggcaccatca
     5821 gctcgggatc gacgggccag ccgagctgct cgtgggcctc aacatagccg cggacccgct
     5881 gggcgacctc cggcgcctcg gtgtcgccga gaaacacgat ccggtggcgg ctgaggcgca
     5941 tcagatgggc cgtcgcgcgg cgaccgccgg ccagattgtc ggagccgacc gagcaatagg
     6001 actggtccgg gagctgggcg ccccagacgg cgaagcgggc gtcgct
//
LOCUS       DASVQZ010000449         4945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_863760, whole genome shotgun sequence.
ACCESSION   DASVQZ010000449 DASVQZ010000000
VERSION     DASVQZ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863760"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..901
                     /locus_tag="VF547_12615"
     CDS             326..901
                     /locus_tag="VF547_12615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8263705.1"
                     /translation="MFDIAVVPGSLRSDSISRRLTGALPTVAPPELRLRTVELAGIEL
                     YNEDLEARPPASWTHFRDSIRRADGILIVTPEYNRSVPGVLKNAVDVGSRPYGASVWA
                     GKPAGIISLSPGGLGGFGANHHLRQSLVFLDCPVLQQPEAYLSQADSLVTGDGTIAER
                     SREFLERFLGAYADWIGRLLPERAAQDRQAA"
     gene            990..1250
                     /locus_tag="VF547_12620"
     CDS             990..1250
                     /locus_tag="VF547_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263706.1"
                     /translation="MGAFKHVYWAVLDQPIFGGETGDVLVVGDDEKAKAEVFAMFERA
                     PFRLLNAGPLINARAIERITMITGALGRSLGTYPRLNWRLLG"
     gene            complement(1366..3813)
                     /locus_tag="VF547_12625"
     CDS             complement(1366..3813)
                     /locus_tag="VF547_12625"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX8263707.1"
                     /translation="MDAPTLGRGTAGEAGESLELPIEGMTCASCVRRVERAIGAVPGV
                     SGVSVNLATEKASVSFEGGRADPGAVAEAIFKAGYMPGRKTTELRIEGMTCASCVGRV
                     EKALGAVPGVQKAQVDLATETARVEGVATATARDLIAAVEKAGYSASLRVPSAAAQSG
                     AEEARRDAASSLELRHVLIAAALSLPLVLPMLLSPFGIELAVPGWLQLLLATPVRFWL
                     GARFYRAGWKAVRAGAGNMDLLVAIGTSAAYGLSVYQLLRPAHHDAMAHYYFEASAMV
                     VTLVLLGKWLEGRAKRQTGSAIRALMALRPERARLVKNGVEAEIPVEQVAVGDLVRVR
                     PGERIPVDGRLIEGITSVDESMLTGESLPVSKGVGDKLTGGSINGEGLVLIETIAVGA
                     ETTLARIVRLVEDAHGAKAPIQRLVDKVSAVFVPVVLGIAVSTFAGWLIAGAGVEVAI
                     LNAVAVMVIACPCALGLATPTAIMAGTGVAARAGVLIKDAEALETAHRVDTVAFDKTG
                     TLTEGKPSLVGLVPAEGVDAGRLLQVAAGIQSGSEHPLAKAVMAAAAVQEVSPLPVQD
                     MQALPGRGIAASIQDGRLALGSTRLMEEEGVDISSLADAAAAFEKEGRSVSWLAELTP
                     GRRLLGLLAFGDEAKASAREAVEALHRRGIRSVMLTGDNPGSANAAAATLGIDHVVAN
                     VLPEGKAEAVAALKEGKGAVAMVGDGVNDAPALAAADVGIAMSTGSDVAMHAAGITLM
                     RGDPRLVADAIDISSRTYSKIRQGLFWAFIYNLVGVPLAALGFLSPVLAGAAMALSSV
                     SVVGNALILRRWRPSAT"
     gene            3992..4195
                     /locus_tag="VF547_12630"
     CDS             3992..4195
                     /locus_tag="VF547_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX8263708.1"
                     /translation="MVEITVPGMSCGPCGRAVTSAVQGVDPGARVEIDLETKLVAIES
                     GADVAAIAGAIEDAGYKVEREPA"
     gene            4270..>4945
                     /gene="dmeF"
                     /locus_tag="VF547_12635"
     CDS             4270..>4945
                     /gene="dmeF"
                     /locus_tag="VF547_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEX8263709.1"
                     /translation="MHSHSAGPWHHDHLFLGENAQGHERRTWLVVGVTIAAMVAEVGA
                     GLAFGSMALLADGLHMATHAGALTLAGAAYAYARLNAEDPRFAFGTGKVGDLAAFASA
                     VVLAMVALLIGWESLERMTAPAKIDYPEAMAVGAAGLLVNLVSAWLLHPSEQSHDPGA
                     SDHRHDHNLRGAYLHVLADALTSVLAIAALLAGSFYGWTWLDPAVGLVGAIVIARWSV
                     GLMKESG"
BASE COUNT          780 a         1762 c         1637 g          766 t
ORIGIN      
        1 gaggccagcg gcagccccgg caagatcgtc atcgcgatct aggcgctgca gggcgacgcc
       61 gcccgccggt ctcttcgtcg catcggcggc cccgggcctc ccggttgcga gcacgcggcg
      121 acggccacgc cccagcgacc ggagcgccct ccctcacgcc gctccggcca gtccgcgcca
      181 gccgccgcaa ggttgctctg cccttccaga tgtcgccgaa gcgagctcgt ccttgcctcc
      241 gtgcaagggc cgcttgcgca atcggccaat tccggccgcg ccccccacgt cttatccaca
      301 tccggctaac cgacaggagg tccagatgtt tgacatagcc gtagtgcccg gcagcctaag
      361 gtcggactcg atcagccggc gcctgacggg ggcgttgccc acggtggccc caccagagct
      421 tcggctccgc accgtcgagc ttgccgggat tgagctctac aacgaggatc tggaagcccg
      481 tccgcccgca agctggacgc actttcggga ctcgatccgg cgggccgacg ggatcctcat
      541 cgtcactccg gaatataacc gctcggtccc gggcgttctc aagaacgcgg tcgacgtcgg
      601 ctccaggccc tatggcgcca gcgtctgggc cggtaagccg gccgggatca tcagtctctc
      661 gccggggggc ttgggcggct tcggagccaa tcaccacctg cgccagtcgc tggtctttct
      721 cgattgtccg gtcctgcagc agccggaagc ctatctcagc caggccgact cgctcgtcac
      781 gggcgacggc acgatcgcgg agcgctccag ggagttcctc gaacgcttcc tgggcgccta
      841 tgcggactgg atcggccgcc tgctccccga gcgcgccgcg caagatcggc aggccgcctg
      901 attgcgcggc gcatatcgcg aacgcgcttc caacctcgac ggaggacccg atgcaaaggc
      961 cgataggcat attgggcacc ggtcaagtcg tgggcgcttt caaacacgtc tactgggccg
     1021 tcctggacca gcctatcttc ggcggcgaga cgggcgacgt gctggtcgtc ggcgacgacg
     1081 agaaggccaa ggcggaggtc ttcgcgatgt tcgagcgcgc tccgttcagg cttttgaacg
     1141 cgggcccgct gatcaacgct cgagctatcg agcgcatcac gatgatcacc ggcgcgcttg
     1201 gtcgaagcct gggcacctat ccccggctga actggcggct gctgggctga gtcgccgtcg
     1261 gcacgctcag gtggagagcc cggcagctcc gggaagatcg tcgacctgat ccaggcgcca
     1321 tggacggcgg cctcccgctt ccgcagggag acccccgccc ggccttcagg tcgccgaggg
     1381 ccgccagcgg cgcaggataa gcgcattgcc caccacgctg acgctcgaca gggccatcgc
     1441 cgcgccggcg aggacggggc tgaggaagcc gagcgccgcc agcgggacgc cgaccagatt
     1501 gtagatgaag gcccagaaga ggccctgacg gatcttcgag taggtgcggc tggagatgtc
     1561 gatcgcgtcg gcaacgaggc gcggatcgcc gcgcatcaag gtgatcccgg ccgcatgcat
     1621 ggcgacgtcg ctgccggtgg acatggcgat gccgacatcc gccgccgcca gagccggagc
     1681 gtcgttcacg ccgtctccaa ccatcgccac cgcccccttg ccctccttca aagccgcgac
     1741 cgcttccgcc ttcccctcgg gaaggacgtt ggcgacgacg tgatcgatgc cgagcgtcgc
     1801 ggccgccgca ttggcgctgc ccggattgtc gccggtgagc atcacgctgc ggatgccgcg
     1861 gcgatgcagg gcctcgaccg cctcccttgc cgaggctttg gcctcgtcac cgaaggcgag
     1921 gaggccgaga agccggcgac ccggcgtaag ctcggcgagc cacgacacgg accggccttc
     1981 cttctcgaag gccgccgcgg cgtccgccag cgacgagatg tcgacaccct cctcctccat
     2041 cagccgagtg ctgcccagcg ccagccgacc gtcttggatc gaagcggcga ttccgcggcc
     2101 cggcaaagcc tgcatgtcct gcaccgggag cgggctcact tcctggaccg ccgcggcggc
     2161 catgaccgcc ttggcgagcg gatgctcgct tccgctctgg atcccggccg ccacctgaag
     2221 aagccgtcca gcgtccacgc cctcggccgg gaccaggccg acgagcgacg gcttgccctc
     2281 ggtcagagtc ccggtcttgt cgaaggcgac ggtatcgacc cggtgcgccg tctccagcgc
     2341 ctccgcatcc ttgatcagca caccggcccg cgcggccact ccggtgccgg ccatgatcgc
     2401 ggtcggcgtg gccaggccca gggcgcaggg gcaggcgatc accatcaccg cgacggcatt
     2461 gaggatcgcg acctcgacac cggcgcccgc gatgagccag ccggcgaagg tcgaaaccgc
     2521 aatgccgaga acaacgggga cgaagacggc gctgaccttg tcgaccaggc gctggatcgg
     2581 agccttggcg ccgtgcgcgt cctcgacgag gcggacgatc cgggccagcg tcgtttccgc
     2641 gccgacggcg atcgtttcga tgagaacgag gccctcgccg ttgatggagc cgccggtgag
     2701 cttgtcgccg actcctttgc tcaccggcag gctctcgccg gtcagcatcg actcgtcgac
     2761 gctggtgatg ccttcgatga gccggccgtc caccgggatc cgctctcccg gccggactcg
     2821 gacgaggtcg ccgaccgcga cctgctcgac gggaatctcg gcttcaacgc cgttcttcac
     2881 caggcgcgct cgctcggggc ggagcgccat caaagccctg atcgctgagc cggtctgccg
     2941 cttggcacgg ccctcgagcc atttgccgag aaggacgagc gtgaccacca tcgccgaggc
     3001 ttcgaaataa taatgggcca tcgcgtcatg atgggccggc cgaagcagct ggtagacgct
     3061 caggccgtag gccgcggacg tcccgatcgc cacgagcagg tccatgttgc cggcgccggc
     3121 tcgcactgcc ttccacccgg cgcgatagaa gcgggcgccg agccagaacc ggaccggagt
     3181 ggccagcagc agctgcagcc agcccgggac ggcaagctcg atgccgaacg gcgaaagcag
     3241 catcggcagg acgagcggaa gcgagagtgc cgcagcgatg aggacgtggc ggagctccag
     3301 gctgctcgcg gcatcgcgcc tcgcctcctc cgcgccggac tgggcggcag ccgagggcac
     3361 acgcaggctg gccgaatatc cggctttctc gacggccgcg atgaggtcgc gagccgtcgc
     3421 cgtggcaacc ccctcgacgc gagcggtctc ggtagccagg tccacctgcg ctttctgcac
     3481 gccgggaacg gcgcccagag ccttctcgac gcggccgacg cacgaggcgc aggtcatgcc
     3541 ctcgatcctg agctcggtcg tcttgcgacc gggcatgtat ccggccttga agatcgcctc
     3601 cgcgacggcg ccgggatcgg cccggccgcc ctcgaagctc accgaggctt tctcggtcgc
     3661 gagattcacg gagacgccgc tcactcccgg aacggcgcct atggcccttt ccacgcggcg
     3721 cacgcacgag gcgcaggtca tgccttcgat gggcagctcg aggctttcgc cggcctcgcc
     3781 cgccgtcccg cgtcccaatg tcggcgcgtc cacttgtcgt ctcctcgaaa tgagtcgacg
     3841 gccaatctgg tggttgacat cgtgtgaagg tcaagaggcc gattcacgac gtcgatctcg
     3901 gcctcgcgaa gagattcttc atgacgccct tgaccctgac atcgtgtcaa atccgacatg
     3961 tccgttctcg caacctcgat tggagaagga catggttgag attaccgttc cgggaatgag
     4021 ctgcggtccc tgcggccggg ccgtcaccag tgcagtccag ggcgtggatc ccggcgccag
     4081 ggtggagatc gacctcgaaa ccaagctggt cgccatcgag tcgggcgcgg acgtcgccgc
     4141 catcgcgggc gcgatcgaag atgccggcta caaggtcgag cgggagccgg cctgagcggc
     4201 tttaagaggg ccggccccga agcgccgccc ctcgcccgac ccgcgacgat ttccaaggag
     4261 ctatgccaaa tgcactcgca ctccgcgggc ccctggcacc acgaccacct cttccttggg
     4321 gaaaatgccc agggccatga acgacgaacc tggctcgttg tgggggtgac gatcgccgcg
     4381 atggtggccg aagtcggcgc cggcctggcc ttcggctcga tggcgctgct cgccgacggg
     4441 ctgcacatgg cgacccacgc cggcgccctt accctggcgg gcgccgccta tgcctacgcc
     4501 cgcctcaatg cggaggatcc gcgcttcgcc ttcggaaccg gcaaggtcgg cgatctcgcg
     4561 gccttcgcga gcgccgtcgt ccttgcgatg gtcgcgctcc tgatcggttg ggaaagcctc
     4621 gagcgaatga ccgcgcccgc caagatcgac tatccggaag cgatggccgt cggtgctgcc
     4681 ggtctgctgg tgaacctggt cagcgcctgg ctgctccacc cgtccgaaca gagccacgac
     4741 cccggcgcct cggaccaccg ccacgaccac aatctgcgcg gcgcctacct ccatgtcctg
     4801 gccgatgccc tgacctcggt cctcgcgatc gcggcgctgc tggcgggctc cttctacggc
     4861 tggacgtggc tcgatccggc agtcggcctt gtcggcgcga tcgtgattgc gcgctggtcc
     4921 gtcggcctga tgaaagagtc ggggg
//
LOCUS       DASVQZ010000450         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_191680, whole genome shotgun sequence.
ACCESSION   DASVQZ010000450 DASVQZ010000000
VERSION     DASVQZ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191680"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VF547_12640"
     CDS             complement(<1..816)
                     /locus_tag="VF547_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX8263710.1"
                     /translation="MSGTNSYFFCGIGGSGMLPLASIVRARGAEVAGSDRALDQGRTA
                     AKFDYLRAQGIALYPQDGSGPRPGQVLVASAAVEESVPDVQAARRLGLERITRAGLLA
                     RLFNAAATGIAVGGTSGKSTVTGMIAWILHRRGLAPTVMNGAVMKNFVGPDSPFASAL
                     VGSGDLFVSEVDESDGSIALFRPGIAVLNNVSLDHKSMEELRALFGDFLGRSGTAVVN
                     LDDAEARALAPAGAVTYGRRAAEADYRASDIAEEPFAVSFTVTKRQAGETARVR"
     gene            869..1684
                     /locus_tag="VF547_12645"
     CDS             869..1684
                     /locus_tag="VF547_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX8263711.1"
                     /translation="MRIGIVAPSTPILADTAERTTAVAREAFPEVELVFHPQCFLRHR
                     HFAGEDPVRAEAFVEVANDPGFDALWFARGGYGSCRIAEAVLARLGPAAREKAYLGYS
                     DAGYVLAGLYKAGFGQVAHGPMPQDVRREGGEAAVGRALAWLARRDPETLEPSLGETP
                     AAAFNITVFSQLLGTALEPDLAGHVLMLEDVSEHMYRTDRALFHLTGNARVREVAGIR
                     LGRCTEVPDNDPDFGASEEEVFLYWCERSGIPYLGRADIGHDAANKVVPFGRS"
     gene            1878..2147
                     /locus_tag="VF547_12650"
     CDS             1878..2147
                     /locus_tag="VF547_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX8263712.1"
                     /translation="MAKGTTGGGARGGLSRPVTPSADLAAITGSDPLPRSQVVSKMWD
                     YIRTNKLQNPDNKREIVADDKLKKIFGADRCSMFEMNKHLSRHLK"
     gene            complement(2274..2969)
                     /locus_tag="VF547_12655"
     CDS             complement(2274..2969)
                     /locus_tag="VF547_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX8263713.1"
                     /translation="MLALLTFAAFLGWGYARNHPEDVPWTKLDLGRPVGAFTGRKLAA
                     LSGEGPACRALLRRAGIRFAALPPRAPQRGCGYDDAVRFARGGAVSLAYRPADLGTSC
                     PVAAGLALWEWHVVQPAALRRFQVPVDRVEHYGSYSCRRIYGRATGAWSEHARANAVD
                     IAAFVLADGRRVSVAADWKSEGAEGRFLRDVRDGACRLFATTLSPDYNAAHRDHLHLD
                     QAERGAMGWRACR"
BASE COUNT          493 a         1026 c         1147 g          442 t
ORIGIN      
        1 ccggacgcgg gcggtctcgc cggcctgccg cttcgtcacg gtgaaggaga cggcgaacgg
       61 ctcctcggcg atgtcggagg cgcgatagtc ggcctcggcc gcgcggcggc cgtaggtgac
      121 cgcgccggcc ggcgccagag cgcgcgcctc ggcatcgtcg aggttgacga cggcggtccc
      181 ggagcggccg aggaagtcgc cgaacagggc gcgcagctcc tccatcgact tgtgatcgag
      241 gctgacgttg ttgagcaccg cgatgcccgg ccggaagagc gcgatcgagc cgtcgctttc
      301 atcgacctcg ctgacgaaga ggtcgccgga gccgaccagg gcgctcgcaa agggcgaatc
      361 cgggccgacg aaattcttca tgacggcgcc gttcatcacc gtcggcgcca gcccgcgccg
      421 gtgcaggatc caggcgatca tgccggtgac ggtcgacttg ccgctggtcc cgccgacggc
      481 gatgccggtg gcggccgcgt tgaacaggcg cgcgagcagg ccggccctgg tgatccgctc
      541 caggccgagg cggcgggccg cctggacgtc ggggacgctc tcctcgaccg cggccgaggc
      601 gaccagcacc tggcccggcc gaggcccgct tccgtcctgc gggtagaggg cgatgccctg
      661 ggcgcgcaga tagtcgaact tggccgccgt ccggccctgg tcgagggcgc gatccgagcc
      721 ggcgacctcg gcgccccggg cgcgcacgat cgaggcgagc ggaagcatgc cgctgccgcc
      781 gataccgcag aagaaatagg agttggtccc ggacatggcg ccgccctatt gaggtcggcg
      841 cggcatttca aacgctgagg gagggggaat gcggatcgga atcgtggcgc cgagcacgcc
      901 gatcctcgcc gacaccgcgg aacggacgac ggcggtcgcg agggaagcct tccccgaggt
      961 cgagctggtc ttccatcccc aatgcttcct ccgccaccgc catttcgccg gcgaggatcc
     1021 ggttcgagcc gaggcgttcg tcgaggtcgc caacgatccc ggcttcgacg ccttgtggtt
     1081 cgcccgcggc ggctacggct cgtgccggat cgccgaggcg gttctggcca ggctggggcc
     1141 ggcggcgcgg gagaaggcct atctgggcta cagcgatgcg ggctacgtcc tcgccgggct
     1201 ctacaaggcc ggcttcggtc aggtcgcgca cggcccgatg ccccaggacg tgcggcgcga
     1261 gggcggggag gccgcggtgg ggcgggccct ggcctggctc gcgcggcgcg atcccgaaac
     1321 cctggagccg agcctggggg agacgccggc ggcggcgttc aacatcaccg tcttctcgca
     1381 gctgctcgga accgcgctcg agccggacct cgccggccac gtgctcatgc tcgaggacgt
     1441 ctccgagcat atgtaccgga ccgaccgggc gctgttccac ctcaccggca acgcccgggt
     1501 gcgcgaggtc gccgggatcc ggctcggccg ctgcaccgag gtgcccgaca acgaccccga
     1561 tttcggcgcg agcgaggagg aggtgttcct ctattggtgc gaacggtccg gaatccccta
     1621 tctcggccgg gccgacatcg gccacgatgc cgccaacaag gtggtgccgt tcggcaggag
     1681 ctgacgggtc gagtcggtcg atcgccgctg gccgacggga catctggcgc tcgaaaggcc
     1741 cgttcgggac attgatccac agcaaaatcg cgttggaacg gcccttagag gcggtccgac
     1801 gaattccttc cttggaatct cttgcgctcg ccgcgcgttt ggggcttatc agcgaagcat
     1861 tcacaggagg gaaaactatg gcaaagggca ctacaggcgg tggggcgagg ggcggactat
     1921 cgcggccggt gactccgtcg gccgatctgg cggcgatcac gggctcggac ccgctgccgc
     1981 gcagccaggt ggtcagcaag atgtgggact atatccgcac caacaagctg cagaaccccg
     2041 acaacaagcg cgagatcgtc gccgacgaca agctcaagaa gattttcggg gccgaccgct
     2101 gctcgatgtt cgagatgaac aagcaccttt cgcgccatct gaaatagcgc ctttcccgct
     2161 tcggcggaga actggatcgg cctcgcccac ccgggcgggg ccgatctgcg ttcggggagg
     2221 gcttcgacaa gctcagcccg aacggaagca agggtagctc acgccctcga cctctagcgg
     2281 catgcccgcc agcccattgc gccgcgctcc gcctggtcga gatggaggtg gtcgcgatgg
     2341 gcggcgttgt agtcggggga caaggtggtg gcgaacaggc ggcaggcgcc gtcgcggacg
     2401 tcgcggagga aacggccctc ggcgccttcg cttttccagt cggcggcgac gctgacccgg
     2461 cggccgtcgg cgagcacgaa ggcggcgatg tcgacggcat tggccctggc atgctcgctc
     2521 caggccccgg tcgcgcggcc gtagatgcgg cggcagctgt agcttccgta atgctcgacg
     2581 cggtcgacgg ggacctggaa acggcgcagc gcggccggct gcaccacgtg ccattcccac
     2641 aaggccaggc cggcggcgac cgggcagctg gtgccgagat ccgcgggccg gtaggccagc
     2701 gagacggcgc cgccgcgcgc gaagcggacc gcgtcgtcat agccgcatcc ccgctgcggt
     2761 gcgcgcggcg gcagggcggc gaaccggatt ccggcgcggc gaagcagggc tcggcaggcc
     2821 gggccttcgc ctgaaagcgc ggcgagcttg cgcccggtga aggcgccgac gggccggccg
     2881 aggtcgagct tcgtccacgg cacgtcctcg ggatggttgc gtgcatagcc ccagccgagg
     2941 aaggcggcga aggtgagcag cgccagcact aggaagattt ttacccggcg gaggtatttc
     3001 actcgatcaa gactccggac gacgggtgcc ggttgcgtgg aaggacgagg agtgacggcg
     3061 aaccgcctcg cttcaaggca ggtcgagaaa ttgtgggggc gcgccgat
//
LOCUS       DASVQZ010000451         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_864175, whole genome shotgun sequence.
ACCESSION   DASVQZ010000451 DASVQZ010000000
VERSION     DASVQZ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864175"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VF547_12660"
     CDS             <1..450
                     /locus_tag="VF547_12660"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8263714.1"
                     /translation="AVGGGMSSRLFQQVREDRGLAYSVYSQLHPYADAGMFYIYAATA
                     RRESAAAAQLIDEIVHEAAATVTAREVDRVRTQSRAALLMSIESPWGQAHYVARQLAV
                     HGRLVEPAEVIAELEAVTVDQVREAGARMLAGPRARATIGFPAVRAA"
     gene            515..1402
                     /locus_tag="VF547_12665"
     CDS             515..1402
                     /locus_tag="VF547_12665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8263715.1"
                     /translation="MRPGVPAFAGTTIVSEPWADYGLVDSGGGRKLERYGRFRFVRPE
                     PQALWAPAASDWDWDGEFVPGSDEDGGGRWQFARPVPREGWELAWEEVRFRAQATPFR
                     HLAFFPDMAPQWAWMRERLKEGGEALNLFGYTGVGTLAMAAKGARVTHVDASRKSVEA
                     GKENALLSGLADRPVRWLVDDAAKFAAREVRRGRRYDGIILDPPKYGRGPNGEVWKLE
                     EGLPGLVADCRRLLDSDSRFLVLTVYAVRMSALAIGELLSQALGDLGGRVECGDMAVL
                     EEARGLLLPTAIFARWSRP"
     gene            complement(1449..2090)
                     /locus_tag="VF547_12670"
     CDS             complement(1449..2090)
                     /locus_tag="VF547_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX8263716.1"
                     /translation="MSALVLRELRLAAAGGRALLPLVFFLLVATLFPFAVGPDAPVLA
                     RIGGGALWTAALLAALLPVDRLVEPDRASGALDQLAVRGLAEESVALAKLAGHWLSFG
                     PPLMFAALPAAALMKLPGPVLIRLELGLLAGTPGLAALSLIVASLTSGLRTTGALAGL
                     LMLPLAVPILIFGAGLVAPGGEGGALKLLAAASLLLVAIAPFAAGTAIRAARD"
     gene            complement(2087..>2454)
                     /locus_tag="VF547_12675"
     CDS             complement(2087..>2454)
                     /locus_tag="VF547_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8263717.1"
                     /translation="ALADEGLALDPRLGLRRALAFWAGLDGADPGPAMAAMGLAALAG
                     VPVRLLSPGQRTRAALARVLASGARLWLLDEPANGLDCDGEARLEAAIAAHRAGGGGV
                     LAASHQPLALPGAARVALG"
BASE COUNT          324 a          894 c          957 g          279 t
ORIGIN      
        1 gcggtcggcg gcggcatgtc gtcgcgcctg ttccagcagg tgcgcgagga tcgcgggctc
       61 gcctactcgg tctattccca gctccatccc tatgccgacg ccggcatgtt ctacatctac
      121 gcggccaccg cccggcgcga atcggccgcc gccgcgcagc tgatcgacga gatcgtccac
      181 gaagccgcgg cgaccgtgac cgcgcgcgag gtcgaccggg tccgcaccca gtcgcgggcg
      241 gcgctgctga tgtcgatcga gagtccctgg ggacaggccc attacgtcgc ccgccagctc
      301 gccgtccacg gccgcctggt cgagccggcg gaggtgatcg ccgagctcga ggccgtgacg
      361 gtcgaccagg tgcgcgaggc gggggccagg atgctggccg gcccgcgggc gcgggcgacg
      421 atcggctttc ccgccgtgag ggcggcgtga ccgctccccg aggccgtcac cccagcgaag
      481 gctggggtcc cggggggtga aaggctccga gaccatccgc cctggggtcc cagccttcgc
      541 tgggacgacg atcgtttccg agccctgggc cgattacggc ctcgtcgaca gcggcggtgg
      601 gcgcaagctc gagcgctacg ggcgattccg cttcgtacgg ccggagccgc aggctctgtg
      661 ggcgccggcc gcctcggact gggattggga cggggagttc gttcccggct ccgacgagga
      721 cggtggcggc cgctggcagt tcgcccggcc ggtgccgcgc gagggctggg agctggcctg
      781 ggaggaggtc cgcttccgcg cccaggcgac gcccttccgc cacctcgcct tcttcccgga
      841 catggcgccg caatgggcct ggatgcgcga gcgcctgaag gagggcggcg aggccctcaa
      901 cctgttcggc tataccggcg tcggcaccct ggcgatggcg gcgaagggcg cgcgcgtgac
      961 ccatgtcgac gcctcgagga agtcggtcga ggccggcaag gagaatgcgc tcctctccgg
     1021 cctggccgac cggccggtcc gctggctggt cgacgacgcc gccaagttcg ccgcgcggga
     1081 ggtgcggcgc gggcggcgct acgacggcat catcctcgac ccgcccaaat atggccgcgg
     1141 gcccaacggc gaggtctgga agctcgagga ggggctcccc gggctggtcg ccgactgccg
     1201 gcgcctgctc gactccgaca gccgcttcct ggtgctgacc gtctacgcgg tgcgcatgtc
     1261 ggcgctggcg atcggcgaac tgctgagcca ggcgctgggc gatctcggcg gccgggtgga
     1321 gtgcggcgac atggccgtgc tcgaggaggc gcgcgggctg ctcctgccca cggcgatctt
     1381 cgctcggtgg tcgcggccct agcccgggcg ggccgggtcg atccgaggtc ggggaaggcg
     1441 ccggcgctct agtcccgagc cgcccggatc gcggtgccgg ccgcgaaggg ggcgatggcg
     1501 acgaggagga gcgacgccgc cgccagcagc ttcagcgccc cgccctcccc ccccggggcg
     1561 accagcccgg ccccgaagat caggatcggg accgcgagcg gcagcatcag caggcccgcc
     1621 agggcgccgg tcgtccgaag ccccgaggtc agcgaagcga cgatcaggct gagcgcggcg
     1681 agccccggcg ttcccgcgag gagcccgagc tcgaggcgga tcagcaccgg gcccggcagc
     1741 ttcatcagcg ccgcggcggg gagggcggcg aacatcaggg gaggtccgaa gctcagccag
     1801 tggccggcga gcttggcgag cgcgacgctc tcctcggcaa ggccgcgcac cgccagctgg
     1861 tcgagcgctc cggaagcccg gtcgggttcg acgaggcggt cgaccggcag aagagcggcg
     1921 agcagggccg cggtccacag ggcgccgccg ccgatccggg cgaggaccgg cgcgtcgggg
     1981 ccgaccgcga acgggaaaag cgtcgcgacc agcaggaaga agacgagcgg aagcagggcg
     2041 cgtccgccgg ccgcggcgag ccggagctcg cgcaggacga gggcgctcat cccagcgcca
     2101 cccgcgccgc cccgggcaag gcgagcggct ggtgcgaggc ggcgagcacg ccgccgccgc
     2161 cggcgcggtg ggcggcgatc gccgcctcca gccgcgcctc gccgtcgcag tcgaggccat
     2221 tggcgggctc gtcgagcagc cacagccggg ctccgctcgc cagcacccgc gccagcgccg
     2281 cgcgcgtgcg ctggccgggc gacagcagcc gcaccggcac cccggcgagc gcggcgaggc
     2341 ccatcgcggc catcgccggc cccgggtccg ccccgtcgag gccggcccag aatgcgagcg
     2401 ctcggcgcag gccgagcctc gggtcgagcg cgaggccctc gtccgcgagc gccg
//
LOCUS       DASVQZ010000452        10092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_480494, whole genome shotgun sequence.
ACCESSION   DASVQZ010000452 DASVQZ010000000
VERSION     DASVQZ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 10092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10092
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480494"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..886
                     /locus_tag="VF547_12680"
     CDS             83..886
                     /locus_tag="VF547_12680"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEX8263718.1"
                     /translation="MSAFARRVAELTGVAESQLERLAGGDLSEVLLLKRPDGRCTVAK
                     GGPAVGTEAAMLRALAGVGVPTPSVEGEHDGVLLLEHVPNDGVFSPNAWADIGVAVRR
                     LHARTGERYGWPVDYRIGTVALDNRQTRDWPAFWAEQRLMATASILDRPVRERVERLA
                     ARLPQLLPASPPPAFLHGDLWSGNILVSGGRVVALIDPACYGGHSEVDLAMLTLFEEP
                     PPDFWEAYGPLEPGWRERRPIYQLFPALVHLRLFGPTYADLVDRLLGRC"
     gene            complement(911..1444)
                     /gene="thpR"
                     /locus_tag="VF547_12685"
     CDS             complement(911..1444)
                     /gene="thpR"
                     /locus_tag="VF547_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX8263719.1"
                     /translation="MHRLFVAIRLPSPVRVRLLGLMGGVAGARWLGEDQLHLTLRFIG
                     EVDRHLARDVDAALSGIHSPRFEIALNGLGLFERRGEPVTLWAGVAPQQPLHALHKKI
                     DQALVRVGVEPDRRAYSPHVTIARLPRGAGPVQSLIEHSGGVAGPPFEVGDFCLYESR
                     LTPQGPVYTIVERYSLV"
     gene            1624..2361
                     /locus_tag="VF547_12690"
     CDS             1624..2361
                     /locus_tag="VF547_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX8263720.1"
                     /translation="MANGFDPRATASPQTAGTVGRESVAYDAGLRSYMLSVYNYMASG
                     VLLTGIVALLFARSGLAAELFINGGILKWVVILAPLGLVMWLSFGINRLSTGSAQALF
                     WVYAAAVGLSLSVIFLVYSPTSIAKTFFATAAAFASLSLWGYTTKKDLSGWGNFLLMG
                     VFGLFGALLINVFWQNNTFDLAISAVGVLIFAGLTAYDTQKIKSTYFVVRGSEVAGKA
                     AIMGALELYLDFINLFLFLLRFMGSRD"
     gene            2464..2853
                     /locus_tag="VF547_12695"
     CDS             2464..2853
                     /locus_tag="VF547_12695"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 family protein"
                     /protein_id="HEX8263721.1"
                     /translation="MDLNLVWATLAGAVLGSLADWLFAGVIFHGRYDLHPDTWRRGAE
                     GKRITAAQVLALITSAAFVALADKLGQVDLRGALKLAAMIWLIAPLPLLAANAMFIKV
                     DHLVTASHAAGWLAKLLLIGATTAWFL"
     gene            complement(2854..3729)
                     /locus_tag="VF547_12700"
     CDS             complement(2854..3729)
                     /locus_tag="VF547_12700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8263722.1"
                     /translation="MSDLRAAAIALYDRFTHEGIDRRAFMAELTRLAGSAAAASVLLA
                     SIAADPAAAALVPERDTRLRTSRMSWPVGGGRIMKGYQAEPRKASGPLGSMLVIHENR
                     GLNGHVEDVARRLAVAGYRAVAPDFLSPVGGTPANEDSAREAIGKLDLGQAAADAVAT
                     LRLLKSLPGSNGKAGAVGFCWGGGMVNRVAVAAGDLLDAGVPFYGPAPDPSLAPQVRA
                     PLQLHYAGLDERVNKTGLPWRDALQAAGKTVEAHVYPGVNHAFHNDTSAERYDKAAAT
                     LAWQRTLDFFGRYVG"
     gene            complement(3731..4381)
                     /locus_tag="VF547_12705"
     CDS             complement(3731..4381)
                     /locus_tag="VF547_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8263723.1"
                     /translation="MSDTSLTFYTNPMSRGRIVRWMLEEVGQPYRTEILEYGTTMKAP
                     AYLAVNPMGKVPAIKHCDTVVTEAGAICAYLADAFPDAGLAPPSGNPRRGPYYRWMFF
                     AAGPVEAAITGKSLGLLAPPDKKMMAGYGSFEETIDTLERCVGSGPYICGDQFTAADV
                     YVGSQIGWGMMFGSIDKRPAFTDYFGRISSRPAAIRAREIDDALMPEQSPPEPNAD"
     gene            complement(4410..4883)
                     /locus_tag="VF547_12710"
     CDS             complement(4410..4883)
                     /locus_tag="VF547_12710"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8263724.1"
                     /translation="MRFPFGTLAPLGAFLLAACATSNGGSAADGEAQVRAGIRAIADA
                     AAERDVDRTLAVVSRDLRLIHPRRGEVRYDDFEAGIRAGLSGPARLSVTAEVDDVVVS
                     GDMAVASITWRTAITAPDGTVTRRGERDQEVWRREADGKWRLLRGASFPLPAVAE"
     gene            complement(4883..7219)
                     /gene="clpA"
                     /locus_tag="VF547_12715"
     CDS             complement(4883..7219)
                     /gene="clpA"
                     /locus_tag="VF547_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX8263725.1"
                     /translation="MPSFARELEQTLHTALAEASGRRHEYATLEHLLLALTEDAHASK
                     VMSACGVDLKELREAVRAYLDSELEALKVDGQTDPSPTSGFQRVVQRAILHVQSSGRD
                     EVTGANVLVALFSERESYAVYFLQQQDMSRLDAVSYISHGVGKGETAAEPQEVKGTED
                     TKPEEGKKKNESALKQFTVNLNEKALSGRVDPLIGRGPEVDRTVQILCRRSKNNPLYV
                     GDPGVGKTAIAEGLARKIVEGDVPEVLKEAVIYALDMGALLAGTRYRGDFEERLKAVV
                     TELEKLPNSILFIDEIHTVIGAGATSGGAMDASNLLKPALSGGTIRCIGSTTYKEFRN
                     HFEKDRALLRRFQKIDVNEPTIEDTVKILGGLRSAFEEHHKVKYTTDAIKAAVELSAR
                     YINDRKLPDKAIDVIDEVGAMQMLVPPSKRKKTITTKEIEAVISTIARIPAKSVSADD
                     KKTLEHLDRDLKRVVFGQDKAIEVLSSAIKLSRAGLRDPDKPIGNYLFSGPTGVGKTE
                     VARQLASIMGIPLQRFDMSEYMERHSVSRLIGAPPGYVGYDQGGLLTDAVDQHPHSVL
                     LLDEIEKAHPDLFNILLQIMDNGRLTDHHGKTVDFRNVILIMTTNAGASDMAREGLGF
                     GNNTREGEDEEAIKRMFTPEFRNRLDAIVPFSYLPTEVVARVVDKFILQLELQLADRN
                     VHIDLDDEARKWLTARGYDKLYGARPMGRLVQEKIKQPLAEELLFGKLIHGGEVKVTI
                     KDDAPSFEMVAAAPKPSKKKGKKAAEPEGEQGQPEEQA"
     gene            complement(7347..7544)
                     /locus_tag="VF547_12720"
     CDS             complement(7347..7544)
                     /locus_tag="VF547_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX8263726.1"
                     /translation="MDLDELFPSKPDDPLILLGRQDLDPLSVDELHARIEALEAEIVR
                     VRAKIDAAVNHRASAEQLFKK"
     gene            7648..8628
                     /locus_tag="VF547_12725"
     CDS             7648..8628
                     /locus_tag="VF547_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX8263727.1"
                     /translation="MMAIDPVRPGGPEVLEPVERPVPRPVLGEVLIRVAAAGVNRPDV
                     MQRLGFYPPPPGAPTIPGLEVAGTVVELGEAADSALLGQSVCALVAGGGYAEYCAAPA
                     GQCLPVPAGFSLVEAAALPETFFTVWTNLFQRGGAKAGDAVLVHGGTSGIGTTAILLG
                     KAFGLIIIVTAGSDDKCARALEIGAACAINYRTQDFVERVREATAGQGVAIVLDMVGG
                     DYIPGNLACLSEEGRHVSIAAQRGPKAEIPLWEVMRKRLVLSGSTLRARSVAFKAALA
                     EELRREVWPHLEAGPIRPVIHSTFPLADAAAAHRLMDSGEHVGKIVLEVA"
     gene            complement(8646..9404)
                     /locus_tag="VF547_12730"
     CDS             complement(8646..9404)
                     /locus_tag="VF547_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263728.1"
                     /translation="MAITALIAAAPASAERIRTYWRLKPVSAESPTSVATGVPFFQQK
                     LLPVGLVALTEPVALGSTTLPAGTFLYLVFNDEGKIGYCTIKDRSPGNKARSLFIPIL
                     DQRPCLVDGDGDGRFDKTFAVYDKYGGPPTARGSINGAQPLPATAGYRRVDVDQFPSP
                     LTVALKLLGKTTPAKMRLDLVFSRNLGAWPQVRAVPVGDGAVFQVMNAEVRVLALNAG
                     IATVRLRWANDVYLSSNNRNTLFWGRLPAFVPRD"
     gene            9525..>10092
                     /locus_tag="VF547_12735"
     CDS             9525..>10092
                     /locus_tag="VF547_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8263729.1"
                     /translation="MTEKLILHEYSQSGNCYKIRLTAALLGLDLERREYDILRGETRT
                     PRFLAEVNPNGRIPVLQVGERFIPESNAACFYLADGTRLVPDDRFDRADMLRWMFFEQ
                     YNHEPNVATLRFWLAFVGEERLSDLQRSLLPGKREAGEAALRLMDQHLAGRRFFVGDR
                     ISLADVALYAYTHVAEEGGFDLGRYPAVA"
BASE COUNT         1520 a         3325 c         3507 g         1740 t
ORIGIN      
        1 gcgaggcgac ccatttcgac gccgcctggg cggacgtggt cgagggcgcc gaggcactcg
       61 cgcgccggat cgcctcgggg ggatgagcgc cttcgcccgc cgggtcgccg agctgaccgg
      121 cgtcgccgag agccagctcg aacgtctcgc cggcggcgat ctttccgagg tgctgctgct
      181 gaagcggccc gacgggcggt gcaccgtcgc caagggcggg ccggcggtgg gcacggaagc
      241 ggcgatgctg cgcgcgctgg ccggcgtggg cgtgccgact ccgtcggtcg aaggcgagca
      301 tgacggggtg ctgctgctcg agcatgtgcc caacgacggc gtgttcagcc cgaacgcctg
      361 ggccgacatc ggggtcgcgg tccggcggct gcacgcccgg accggcgagc gctacggctg
      421 gccggtcgac tatcggatag gcacggtcgc gctcgacaac cgccagacac gcgactggcc
      481 agccttctgg gcggagcagc ggctgatggc gaccgcgagc atcctcgacc ggcctgtccg
      541 cgagcgggtg gagcggctgg ccgcgcggct gccccagcta ctgccggcct cgccgccgcc
      601 ggccttcctc cacggcgacc tgtggagcgg caatatcctc gtctccggcg gcagggtcgt
      661 cgcactgatc gatcccgcct gctatggcgg ccacagcgag gtcgacctcg ccatgctgac
      721 cttgttcgaa gagccgccgc ccgacttctg ggaagcctat ggtccgctcg agccgggctg
      781 gcgggagcgg cggccgatct accagctctt tccggccctg gtgcatcttc gcctgttcgg
      841 gccgacctac gcggacctgg tggacaggct gcttggccgc tgctgactcc ccctccgacg
      901 gcgagggcgc tcagaccagc gaatagcgtt cgacgatggt gtagacgggc ccctggggag
      961 tgaggcggct ctcgtagaga cagaaatcgc cgacctcgaa cggcgggccg gcgacgccgc
     1021 cggaatgctc gatcagcgac tgcaccggcc cggcgccccg ggggagccgg gcgatggtga
     1081 cgtggggcga ataggcgcgc cgatccggct cgacgccgac ccggaccagg gcctgatcga
     1141 ttttcttgtg cagggcgtgg agcggctgtt ggggagcgac gccggcccag agcgtgaccg
     1201 gctcgccgcg ccgctcgaac aggcccaggc cgttcagcgc gatctcgaag cgggggctgt
     1261 ggatgccgct cagcgcggcg tcgacgtcgc gggcgagatg ccggtcgacc tcgccgatga
     1321 agcgcagggt gaggtggagc tggtcctcgc ccagccagcg ggcgccggcg accccaccca
     1381 tcaggccgag gagccggact cgcaccgggg aggggaggcg gatcgcgacg aacaagcggt
     1441 gcatcgtcgg cgcatacgtc atcccggcgg cggcgggaat tgagaacccc cggcgcgcct
     1501 cccgcttttc cgaggccatg gcgggacagg ctcgagcgtc cggttttcct tgaatgtacc
     1561 cctcaatctc ccgatattgg ccgcagcggc gccgcatact cgccgccaaa ggagagactt
     1621 caaatggcta atggatttga cccgcgtgcg accgcctcgc cgcagacggc cggcacggtc
     1681 ggccgggaga gcgtcgctta cgacgcgggc ctgcggtcct acatgctgtc ggtttacaac
     1741 tacatggcgt ccggagtgct gctcaccggc atcgtcgccc ttctgttcgc ccgttcgggc
     1801 ttggccgccg agctgttcat caacggcgga atcctgaagt gggtggtgat cctcgccccg
     1861 ctgggcctgg tgatgtggct cagcttcgga atcaaccggc tctcgaccgg ctcggcgcag
     1921 gccctgttct gggtctatgc ggcggccgtc gggctctccc tgtcggtgat cttcctggtc
     1981 tactcgccga cctcgatcgc caagaccttc ttcgcgacgg ccgcggcctt cgccagcctc
     2041 agcctctggg gctataccac gaagaaggat ttgagcggct ggggcaattt cctgctgatg
     2101 ggcgtgttcg gcctgttcgg cgccctgctc atcaacgtct tctggcagaa caacacgttc
     2161 gacctcgcga tcagcgcggt gggggtgctg atcttcgccg gcctgaccgc ctatgacacc
     2221 cagaaaatca aatcgaccta cttcgtcgtc cggggaagcg aggtcgccgg caaggccgcg
     2281 atcatgggag cgctcgagct gtacctcgat ttcatcaacc tcttcctctt cctgctgcgc
     2341 ttcatgggca gccgcgactg agcgagtgcg acgatcggct caagggaggc ccggcggcga
     2401 cgccgggcct ttccttttgc cgcttccgct tccgtgctag cttggcgtcg aagggggtga
     2461 gctatggacc tcaaccttgt ctgggcgacg ctggccggag ccgtgctcgg ctcgctggcc
     2521 gactggctgt tcgccggcgt gatctttcac ggccgctacg accttcatcc cgacacctgg
     2581 cgccgaggcg ccgagggcaa gcggatcacg gcggcgcagg tcctggcgct gatcacctcc
     2641 gcggccttcg tggcgctggc ggacaagctc ggccaggtcg acctgcgcgg cgccctcaag
     2701 ctcgccgcga tgatctggct gatcgcgccg ctgcccctgc tcgcggcgaa cgcgatgttc
     2761 atcaaggtcg accatctggt gaccgcgagc cacgccgcgg gctggctggc gaagctgctc
     2821 ctgatcggag cgacgaccgc ctggttcctc tagtcagccg acgtagcggc cgaagaagtc
     2881 gagggttcgc tgccaggcca aagtcgccgc ggccttgtcg taccgctcgg ccgacgtgtc
     2941 gttgtggaag gcgtgattga ctccggggta gacgtgggcc tccacggtct tccccgccgc
     3001 ctgcagagcg tcgcgccagg gcaggccggt cttgttcacg cgctcgtcga ggccggcata
     3061 atggagctgc aggggagcgc gcacctgcgg cgccagcgac gggtcgggcg cggggccgta
     3121 aaagggcacg ccggcgtcga gcaagtcgcc cgcggcgacc gcaacccggt tgaccattcc
     3181 tccgccccag cagaagccga cggcaccggc cttgccgttg ctccccggca gcgacttcag
     3241 gagtcgaagg gtcgcgaccg catccgccgc ggcctggccc agatcgagct tgccgatggc
     3301 ctcgcgcgcg gaatcctcgt tcgccggagt gcctccgacc ggcgacagga aatcgggcgc
     3361 caccgcccgg tagccggcca cggcgagccg ccgggcgaca tcctcgacat ggccgttcag
     3421 cccgcgattc tcgtggatga ccagcatcga gccgagcggg ccggaggcct tgcgaggctc
     3481 ggcctgatag cccttcatga tcctgccgcc gccgaccggc cagctcatcc ggctcgtccg
     3541 caagcgcgtg tccctctcgg ggaccagggc ggcggcggcg gggtcggcgg cgatcgaggc
     3601 gaggagcacc gatgcggccg cggcgctgcc ggcgaggcgg gtcagctcgg ccatgaaggc
     3661 gcgccggtcg atgccctcat gggtgaagcg gtcgtaaagg gctatggcgg cggcgcgcag
     3721 gtcgctcatc tcagtccgcg ttcggctcgg gaggggactg ctcgggcatc agcgcgtcgt
     3781 cgatctcgcg cgctcggatc gcggccgggc ggctgctgat gcgcccgaaa tagtcggtga
     3841 aggccggccg cttgtcgatc gaaccgaaca tcatccccca gccgatctgc gacccgacat
     3901 agacgtccgc ggcggtgaac tggtcgccgc agatataggg gcccgacccg acgcagcgct
     3961 ctagcgtgtc gatcgtctcc tcgaagctcc cgtagccggc catcatcttc ttgtccggcg
     4021 gcgcgagcag gccgagcgac ttgccggtga tcgccgcctc gaccggaccg gcggcgaaga
     4081 acatccagcg ataataaggc ccgcggcggg gattgcccga aggcggggcg agacccgcat
     4141 cgggaaaggc gtcggcgaga taggcgcaga tcgcgcccgc ctcggtgacg acggtatcgc
     4201 aatgcttgat cgccggcacc ttgcccatgg ggttcacggc cagataggcc ggagccttca
     4261 tggtcgttcc gtattcgagg atctcggtgc ggtagggctg cccgacctcc tcgagcatcc
     4321 agcgcacgat ccgcccccgc gacatcgggt tggtgtagaa ggtgaggctg gtgtcggaca
     4381 tggcgcggct ccctggtcga agcagctgat tactccgcaa ccgccggcaa agggaaggat
     4441 gcgccgcgaa gaaggcgcca ttttccgtcc gcctcgcgcc gccagacctc ctggtcgcgc
     4501 tcgccgcgcc gggtcaccgt tccgtccggg gccgtgatcg ccgtgcgcca ggtgatcgag
     4561 gccaccgcca tgtcgccgct gacgacgacg tcgtcgacct cggcggtcac cgagagccgg
     4621 gccgggccgc tcagcccggc gcgaattccg gcttcgaaat cgtcgtagcg cacttcgccg
     4681 cgccgggggt ggatcaagcg caggtcgcgc gagacgacgg cgagggtcct gtcgacgtcg
     4741 cgctccgccg cggcgtcggc gattgcccgg atgcccgcgc gcacctgggc ttcgccatcc
     4801 gccgccgacc cgccattgct ggtggcgcag gcagccagga ggaacgcgcc gagcggggcg
     4861 agcgtcccga aaggaaagcg catcaggcct gctcttcggg ctggccctgc tctccttccg
     4921 gctccgcggc tttcttgcct ttcttcttcg acggcttggg cgcggcggcg accatctcga
     4981 agctgggcgc gtcgtccttg atcgtgacct tgacctcgcc gccgtggatc agcttgccga
     5041 agagcagctc ctcggcgagc ggctgcttga tcttctcctg gaccagccgg cccatcgggc
     5101 gggcgccgta gagcttgtcg tagccgcgcg ccgtcagcca tttgcgagcc tcgtcgtcga
     5161 ggtcgatatg gacgttgcgg tcggccagct gcagctcgag ctggaggatg aacttgtcga
     5221 cgacccgggc gacgacctcg gtcggcaggt agctgaaggg gacgatcgca tcgagccggt
     5281 tgcggaactc gggcgtgaac atccgcttga tcgcctcctc gtcctcgcct tcgcgggtgt
     5341 tgttgccgaa gccgaggccc tcgcgcgcca tgtcgctggc gcccgcattg gtggtcatga
     5401 tcaggatgac gttgcggaag tcgaccgtct tgccgtgatg gtcggtcagc ctcccgttgt
     5461 ccatgatctg gaggaggatg ttgaacaggt ccggatgcgc cttctcgatc tcgtcgagca
     5521 ggagcaccga atggggatgc tggtcgaccg catcggtcag aaggccgccc tggtcgtagc
     5581 cgacatagcc gggaggcgcg ccgatcaggc gggagacgct gtggcgctcc atatattcgg
     5641 acatgtcgaa ccgctggagc gggatgccca tgatcgaggc gagctggcgc gcgacctcgg
     5701 tcttgccgac gccggtcggg cccgagaaga ggtagttgcc gatcggcttg tcgggatcgc
     5761 gaaggccggc tcggctcagc ttgatcgcgg aggagagcac ctcgatcgcc ttgtcctggc
     5821 cgaagacgac ccgcttcaag tcgcggtcga gatgctcgag cgtcttcttg tcgtcggccg
     5881 agacgctctt cgccgggatc cgggcgatgg tcgagatcac cgcctcgatc tccttggtgg
     5941 tgatcgtctt cttgcgcttg gagggcggca ccagcatctg catcgcgccg acctcgtcga
     6001 tgacgtcgat cgccttgtcg ggaagcttgc ggtcgttgat gtagcgggcg gaaagctcga
     6061 ccgccgcctt gatcgcatcg gtcgtgtact tgaccttgtg atgctcctcg aaggcggagc
     6121 gcaggccgcc gaggatcttc accgtatcct cgatcgtcgg ctcgttgacg tcgatcttct
     6181 ggaagcggcg gagcagcgcc cggtccttct cgaaatggtt gcggaactcc ttgtaggtgg
     6241 tcgagccgat gcagcggatc gtcccgcccg aaagcgcggg cttcaggagg ttcgaggcgt
     6301 ccatcgcgcc gcccgaggtg gctccggcgc cgatgacggt gtggatctcg tcgatgaaca
     6361 ggatcgagtt gggaagcttc tcgagctccg tcaccaccgc cttcagccgc tcctcgaagt
     6421 cgccccgata gcgggtgccg gcgaggaggg cgcccatgtc gagcgcgtag atgaccgcct
     6481 ccttgaggac ctcgggaacg tcgccctcga cgatcttgcg ggcgaggcct tcggcgatcg
     6541 ccgtcttgcc gactccggga tcgccgacat agagcgggtt gttcttcgag cggcggcaga
     6601 ggatctggac ggtgcggtcc acctccggcc cgcggccgat cagcggatcg acccggccgc
     6661 tcagcgcctt ctcgttcaga ttgacggtga actgcttgag agccgattca ttcttcttct
     6721 tgccctcttc gggcttggta tcctcggtcc ccttgacctc ctgcggctcg gcggcggtct
     6781 cgcccttgcc gacgccgtgg ctgatgtagc tgaccgcgtc gaggcggctc atgtcctgct
     6841 gctggaggaa atagacggcg tagctctccc gctcggagaa gagggcgacg aggacgttgg
     6901 cgccggtcac ctcgtcgcgg cccgaggact ggacgtggag gatggcgcgc tggacgacgc
     6961 gctggaagcc gctggtcggc gagggatcgg tctggccgtc gaccttcaga gcctcgagct
     7021 cgctgtcaag ataggcgcga accgcttcgc gaagctcctt caggtcgacc ccgcaggcgc
     7081 tcatcacctt ggaggcgtga gcgtcctcgg tcagcgcgag cagcaggtgc tcgagcgtcg
     7141 catactcatg gcggcggccg ctggcttccg cgagagcggt atgcagggtc tgttcgagtt
     7201 cgcgggcgaa ggaaggcaat tcaactactc ctcaaagccc gcgaaaattc acggactaca
     7261 tggactcaat gtcgggcgtc gcgctgccgg catcaagcac atctccaaaa aagctgggga
     7321 aaaggcggaa tgttccccat gcttcgtcat ttcttgaata actgctcggc gcttgcccgg
     7381 tggttaaccg cggcatcgat cttcgcgcgt acccggacga tctcggcttc gagcgcctcg
     7441 atccgggcgt ggagctcgtc gacggagagc ggatcgaggt cctgccgtcc gagcaggatc
     7501 agcggatcgt cgggcttgct ggggaagagc tcgtccaggt ccatgggcgc agaatctccc
     7561 gtgggggggc cgtctgtcaa gcgcaggggc ttgcgcggcc gcgctttcgg cgcgaggcgt
     7621 gaccgcatgg acccaatccc tccgacgatg atggcgatcg accccgtccg cccagggggg
     7681 ccggaggtgc tggagcctgt cgaaaggccg gtgccgaggc cggtgctggg cgaggtgttg
     7741 atccgggtag ctgcagccgg cgtcaaccgg ccggacgtca tgcagcggct cggcttctat
     7801 ccgcctccgc caggagcgcc aacaattccc gggctcgaag tggcgggaac ggtcgtcgaa
     7861 ctcggggaag cggcggattc ggcgctgctc gggcagtcgg tatgcgctct cgtcgctggc
     7921 ggcggttatg ccgaatactg cgccgcaccg gccggacagt gcctgccggt gccggctggc
     7981 ttcagtcttg tcgaggcggc ggcccttccg gaaaccttct tcaccgtctg gaccaatctc
     8041 ttccagcgcg ggggagccaa ggccggcgat gcggtgctgg tccatggcgg cacgagcggc
     8101 atcggcacca ccgcgatcct gctcggcaag gcgttcggat tgatcatcat cgtcaccgcc
     8161 ggctctgacg acaaatgcgc gcgcgcgctg gagatcgggg ccgcttgcgc gatcaactat
     8221 cggacgcagg atttcgtgga gagggtaagg gaggcaacgg ccggccaggg tgtcgcgatc
     8281 gtgctcgaca tggtgggagg cgattatatc cctgggaacc tggcctgcct ttccgaggaa
     8341 gggcggcacg tctccatcgc cgcgcagcgg ggtccgaaag ccgagattcc gctgtgggag
     8401 gtcatgcgca aacgactggt tctgtccggt tccacccttc gcgctcgttc cgttgccttc
     8461 aaggcggcgc tcgccgagga gctcaggcgc gaggtctggc cgcatctgga ggccgggccg
     8521 atccggccgg tcatccattc cactttcccg ctcgccgatg ccgccgcagc ccaccgcctg
     8581 atggacagcg gcgagcatgt cgggaagatc gtgctggagg tggcttaagg cggccctccc
     8641 cgtcgtcagt cgcgcggcac gaaagcaggc aaacgccccc agaacagcgt gttccgattg
     8701 ttcgaagaga gatagacgtc gttcgcccag cgcaggcgca ccgtagcgat tccggcgttg
     8761 agcgcgagga cgcggacctc agcgttcatc acctggaaga cggcgccgtc gccgaccgga
     8821 acggcgcgaa cctggggcca ggctccgagg tttcgcgaaa agaccaggtc caggcgcatc
     8881 ttggcgggag tggttttgcc cagcagcttg agtgccacgg tcagcgggga cgggaattga
     8941 tccacgtcca cccgccgata accggcggtc gccggcagcg gttgggcgcc attgatgctg
     9001 cctcgggcgg tcggcgggcc gccgtacttg tcgtagacgg cgaaagtctt gtcgaagcgg
     9061 ccgtcgccgt ctccgtccac gaggcaaggc cgctgatcga ggatcggaat gaacaggctc
     9121 cgcgccttgt ttccgggcga ccggtccttg atcgtgcaat agccgatctt cccctcgtcg
     9181 ttgaagacga ggtagaggaa agtcccggcc ggcagggtgg tgctgcccaa agcgaccggc
     9241 tcggtcaggg cgaccagccc gaccggaagc agtttctgct ggaaaaaggg gacgccggtc
     9301 gcgaccgagg tcgggctttc cgcggacacc ggcttgagcc gccaataggt cctgatcctt
     9361 tccgcggatg cgggcgcagc cgcgatcaac gccgtgatgg ccaacagagc ttttcccgca
     9421 agccttccct gcatcccgtc cttctccccg accggccgtc ggtatgtgca gtctttcccc
     9481 ggatcaacgt tgcggagccg cgcgacgcgt gccacggatc acgagtgacc gaaaaactga
     9541 tcctccacga atattcgcag tcgggcaatt gctacaagat ccggctgacc gccgcactgc
     9601 tcggactgga cctggagcgg cgcgaatatg acatcctgag gggcgagacg cgcacgcccc
     9661 ggttcctcgc cgaggtcaat ccgaacgggc gcattcccgt cctccaggtc ggcgagcgct
     9721 tcatcccgga gagcaacgcc gcctgtttct atctcgccga tgggacgagg ctggtgcccg
     9781 acgaccgctt cgaccgcgcc gacatgctgc gctggatgtt cttcgagcaa tataatcacg
     9841 agcccaacgt cgcgaccttg cgattctggc tcgccttcgt cggcgaggag cggctgagcg
     9901 acctccagcg cagcctgctg cctggcaagc gcgaggcggg tgaggcggcg ctcaggctga
     9961 tggaccagca tctcgccggc cgccgcttct tcgtcggcga ccggatcagc ctcgccgacg
    10021 tcgcgctcta cgcctacacg catgtggccg aggagggcgg gttcgatctc ggccgctatc
    10081 cggcggtcgc gc
//
LOCUS       DASVQZ010000453         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_571957, whole genome shotgun sequence.
ACCESSION   DASVQZ010000453 DASVQZ010000000
VERSION     DASVQZ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571957"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VF547_12740"
     CDS             complement(<1..654)
                     /locus_tag="VF547_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX8263730.1"
                     /translation="MRIASVLKLAGAASLAVAGAAAAQVSSVDPNAPPPASSSGQASA
                     VPAPPAPQDDYQPVDSGPQAPAPNAPAPASAAPSASERASASIRGDTVPRKDVFSAAE
                     GVFGKGAEGLGDLVEKLLREQGEPTAYITGREAGGAFVVGVRYGSGTLHHQIEGDRPV
                     YWTGPSVGFDAGADASKVFVLVYNLHDSQRLYRRYPAAEGNAYVVGGFTASYLRRGDI
                     "
     gene            815..907
                     /locus_tag="VF547_12745"
     tRNA            815..907
                     /locus_tag="VF547_12745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Ser,seq:gct)
     gene            complement(924..1175)
                     /locus_tag="VF547_12750"
     CDS             complement(924..1175)
                     /locus_tag="VF547_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263731.1"
                     /translation="MALDESWELNERGQVKFAALMGYSMATMPEGGLVRLEYAETLEA
                     IDRRTPSGLQLHLTAPQAREIGEALRRLADTLEEQAQGT"
     gene            complement(1251..2441)
                     /locus_tag="VF547_12755"
     CDS             complement(1251..2441)
                     /locus_tag="VF547_12755"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8263732.1"
                     /translation="MPETNARQRYDAIVLGAGGAGLMCAAVAGQRGRRVLLIDHARDP
                     GKKILISGGGRCNFTNLEAGPDRYLSENPHFARSALSRYRAEDFLALVEGHGIPWHEK
                     KLGQLFCDGSAVQIVDMLLAECPPETVRLQLGSPIRDVSHADGLFRVSVGDAEAEATA
                     LVIATGGPSIPKIGATGFAYDLARRFGLKIVEPRPALVPLTLTGEAALFRTLSGVATD
                     VVARSGKAAFAEAALFTHRGLSGPAVLQISSYWRHGQEIGIDFLPSAAPDCLIALKRA
                     RPQARFPAALATLLPARLAETLAERLGLDGDLGNLTDRRLAEAERRLRDWRFLPSGTE
                     GFAKAEVAAGGIATAALSSRTMEAASIPGLYAIGEAVDVTGWLGGYNFQWAWASGWAA
                     GQAL"
     gene            complement(2441..3085)
                     /locus_tag="VF547_12760"
     CDS             complement(2441..3085)
                     /locus_tag="VF547_12760"
                     /inference="COORDINATES: protein motif:HMM:NF027558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEX8263733.1"
                     /translation="MTRLRPALSLVPALVALLGACQTAAPPPRPRVSVSVEAAEAWRA
                     QATPADAELLDTLPTRWNQALEQARAARLTRRVSAEGALLDPKTALARAAPAPGPYGC
                     RVIRFPAPGTKMRWDQSANFFCFVGAEADGPSLTVDGVPQRLGGYLFEEKGGTRLVFL
                     GAARTRRGALAAYGDAAGTNAIGMVERIGDFRYRLTVPQRGGGLAVYEMVASPR"
BASE COUNT          468 a         1066 c         1207 g          425 t
ORIGIN      
        1 gatgtcgccg cgccgcaggt aggaagcggt gaagccgccg acgacatagg cgttgccctc
       61 cgccgcggga tagcggcggt aaaggcgctg gctgtcgtgg agattgtaga ccagcacgaa
      121 caccttgctg gcgtccgcgc cggcgtcgaa gccgaccgac ggcccggtcc agtagaccgg
      181 gcggtcgccc tcgatctggt ggtggagcgt gcccgagccg tagcgcacgc cgacgacgaa
      241 ggcgccgccg gcttcgcgcc cggtgatgta ggccgtaggc tcgccctgct ccctgagcag
      301 tttctcgacc aggtcgccaa gcccttccgc gccttttccg aagacgcctt cggcggcgct
      361 gaacacgtcc ttgcggggca cggtgtcgcc gcggatcgag gcgctggcgc gctcagaggc
      421 ggacggggcg gcgctggccg gggccggagc gttaggcgcg ggggcctgcg gcccggaatc
      481 gaccggctgg taatcgtcct gcggcgcggg cggcgcgggg acggcgctcg cctggccgga
      541 gctcgaggcg ggcggcggcg cgttggggtc gacggaggag acctgcgcgg cggcggcgcc
      601 ggcgacggcc aggctcgcgg ccccggccag cttcaacacg gacgcgatac gcatggcggc
      661 attcccctct cgctctcggg cgaagctaaa cccggcgcgg ctgtcgcggc aatgaacggc
      721 gcgacgagcc gagtcgttct cgcggcggaa cggccgcgtg gacgcggttg aacccgcggg
      781 aacgcctcgc tatagccgcc gcctcggcac atccggagac gtgggtgagt ggctgaaacc
      841 agcggtttgc taaaccgccg tagggggatt actcctaccg agggttcgaa tccctccgtc
      901 tccgccagcc ttcttgcggc cgctcaagta ccctgcgcct gttcttcgag cgtatcggcg
      961 aggcggcgca gcgcttcgcc gatctcgcga gcctgcggtg ccgtcaggtg gagctggagg
     1021 ccggaaggcg tccgccggtc gatcgcctcg agcgtctcgg catattcgag gcggacgagg
     1081 ccgccctcgg gcatggtcgc catcgaatag cccatcaagg cggcgaactt cacctggccg
     1141 cgctcgttca gttcccaact ttcatccaaa gccatgtccg tgttcctcag aggggcaggg
     1201 gcgccctcta gcggaggccg gcgcgccggc aaaccgcccg gtgatgggcc ctagagcgcc
     1261 tgcccggccg cccagccgct ggcccaggcc cattgaaaat tatagccgcc cagccagccg
     1321 gtgacgtcca ccgcctcgcc gatcgcgtag aggccgggga tagaggcggc ttccatcgtg
     1381 cgcgaggaca aggccgcggt cgcgattcct cccgccgcga cctccgcctt ggcgaagccc
     1441 tccgttccgc tcggcaggaa gcgccagtcg cgcaggcgcc gttcggcttc ggcgaggcgg
     1501 cggtcggtaa ggttgccgag atcgccgtcg aggccgaggc gctcggccag cgtctccgcc
     1561 aggcgagcgg ggagcagagt ggcgagcgcg gcggggaaac gggcctgcgg gcgggcgcgc
     1621 ttcaaagcga tcaggcaatc gggcgcggcg gacggcagga agtcgatgcc gatctcctgc
     1681 ccgtgccgcc agtaggagga aatctgcagg acggcggggc cggaaaggcc gcggtgcgtg
     1741 aagagggcgg cttccgcaaa ggcggccttg ccggaccgcg cgacgacgtc cgtggcgacg
     1801 ccggacaggg tgcggaacaa agccgcctcg ccggtgaggg tcagcggcac gagggcgggg
     1861 cgaggctcga cgattttgag gccgaagcgg cgtgccagat cgtaggcgaa gccggtggcg
     1921 ccgatcttcg gaatggacgg cccgccggtc gcgatcacca gcgccgtcgc ctccgcctcc
     1981 gcgtctccga ccgagacgcg aaagaggccg tcggcgtggc tcacgtcccg gatcggcgac
     2041 ccgagctgca gccggaccgt ctccggcggg cattcggcga gcagcatgtc gacgatctgg
     2101 acggcggatc cgtcgcagaa aagctggccg agcttcttct cgtgccaggg gatgccgtgg
     2161 ccttcgacca gggcgaggaa atcctccgcc cggtagcggc tgagcgcgga gcgggcgaaa
     2221 tgcgggttct cggagaggta acggtcgggc cccgcctcca gattggtgaa gttgcagcgg
     2281 ccgccgccgg aaatgaggat cttctttccc gggtctctcg catggtcgat cagcaggacg
     2341 cgccgcccgc gctggccggc caccgcggcg cacatcagcc cggccccgcc ggcgccgagc
     2401 acgatcgcgt cgtagcgctg ccgggcgttc gtctccggca ctagcgaggc gaagcgacca
     2461 tttcgtagac ggcgaggccg ccgccgcgct ggggcacagt cagccggtaa cggaagtcgc
     2521 cgatccgctc gaccatgccg atggcgttcg tgcccgccgc gtcgccatag gcggcgaggg
     2581 cgccgcgccg ggtccgcgcg gcgccgagga agacgaggcg agtgccgccc ttctcctcga
     2641 acagatagcc gccgaggcgc tgcgggacgc cgtcgacggt gaggctcggc ccgtcggctt
     2701 cggcgccgac gaagcagaag aagttggcgc tctggtccca gcgcatcttc gtaccgggcg
     2761 cgggaaagcg gatgacccgg cagccatagg ggccgggtgc cggcgcggcg cgggcgagcg
     2821 cggtcttggg atcgagcagc gcgccttcgg cggagacgcg gcgggtgagc ctggcggcgc
     2881 gcgcctgctc cagcgcctgg ttccagcgag tgggaagggt gtcgagcagt tcggcgtcgg
     2941 ccggcgtcgc ctgcgcgcgc cacgcctcgg ccgcctccac cgagacgctg acgcggggac
     3001 gcgggggcgg ggcggccgtc tggcaggcgc ccagcaaggc gacgagggcg ggaaccaggg
     3061 atagggcagg gcgaaggcga gtcatgcgtg tctccacgcc ctactgcgcc cgcacatggt
     3121 taacaaaagg taaagcccgc cgcggggcga ggcgcccgcg cggcgg
//
LOCUS       DASVQZ010000454        11943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_92888, whole genome shotgun sequence.
ACCESSION   DASVQZ010000454 DASVQZ010000000
VERSION     DASVQZ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92888"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VF547_12765"
     CDS             <1..484
                     /locus_tag="VF547_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263734.1"
                     /translation="IQLSPPDGRVEVEVRYKNFPIQREVIADDVDNFTFSFPARGRFT
                     VGRNIIGIILSVAVVLGIVLVAFYLGAQGKVLPLALGVVLVATALTLAFAFKDANPLQ
                     QQLVRSTFAIGVGALASQVPGLLNINVTIGTTATISAAGAIAAFVVTFFFVPAGQPQP
                     "
     gene            complement(513..2102)
                     /locus_tag="VF547_12770"
     CDS             complement(513..2102)
                     /locus_tag="VF547_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8263735.1"
                     /translation="MLGGMQDWPLRIMRILDHAAREHGARELVSLYADGTTVRTDWAG
                     IARDSRRLALALERLGIEPGDRVGTLAMNHSRHVVAWYGTIGMGGIIHTINPRLFDDQ
                     LVYIANHAEDRVLFYDRAFAPIVERLKPRWTSIERFICFENGEFDDLLAAEGDDYAWV
                     EGDERDPAMLCYTSGTTGNPKGVLYEHRSTVLHAMVQMSPDVFDLSARAVVLPVVPMF
                     HAAGWALPFCTAMAGGKMVFSTDYTPQAMCDLMKAEGVTHTAGVPTVWLALVNHVDST
                     GEDLGKLTTVHIGGSAAPRAMIEWFMDRGIRVGHAWGMTEMSPLGTVGAGSGDWDELS
                     REQRVALLCKQGRVPFGVELRTVDEAGNVLPRDGRTSGRLQTRGPWIIRQYFQDPAGD
                     VIDEHGWFDTGDVAALHPDGVLQITDRSKDVIKSGGEWISSIDLENAAVGCPGVAEAA
                     AVGVKHPKWDERPLLLVVRKAGAEVTPQQILDHLSGHVAKWWLPDEILFVDSLPHTAT
                     GKLLKTALREQYRDYKLASAA"
     gene            complement(2138..5722)
                     /gene="dnaE"
                     /locus_tag="VF547_12775"
     CDS             complement(2138..5722)
                     /gene="dnaE"
                     /locus_tag="VF547_12775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8263736.1"
                     /translation="MAGPYVPLRIFSSYTMLEGAIDPKAIAKLAKRLDFPAAALTDRN
                     GLYAAMAFTDACREAGVQPIVGTMLAVARPDCAGGPPALDWIALYAQNDRGYDNLCTL
                     VSAAHLGRPVEEPAHVSLDALDGRTDGLIALTAGADGALVRLISQGQDAAADLILERL
                     MSLFPGRLYIEISRRQDEGEQRSEARLVELAYRHDLPLVATNPAAYADAHFHEAHDAL
                     LCIAQSSQLDRDDRRRSSSETWLKPAAEMRRLFADLPEAIENSGVVARRCAFGAPQRR
                     PILPSIAGDVEAEADQLRRDARAGLEARLADRGELTGEERQAYFDRLDYEADIIVRMG
                     FSAYFLIVADFIKWAKENDIPVGPGRGSGAGSVVAWALTITDLDPIRLGLLFERFLNP
                     ERVSMPDFDIDFCETRRGEVIRYVQQRYGSDKVAQIITFGKLKARAVLKDTGRVLQMP
                     YGQVDRLAKAIPNHPTDPWTLDRALNGVSELAAEYKNDAQVRRLFDLAMKLEGLPRHA
                     STHAAGVVIGDRPLDKLVPLYRDPRSDMPVTQFDMKYVEAAGLVKFDFLGLKTLSVLQ
                     KAVQMLAKRGVEADLDRLAWDDPLVYELLQHGDTVGVFQLESEGMRRTLAAVKPTTFE
                     DIIALVSLYRPGPMDNIPTFGARKNGREPIAYPHPLLEPVLKETYGIFVYQEQVMEAA
                     KVLAGFSLGEADLLRRAMGKKIKSEMDAQRAGFVAGCERVNSIPKAKANELFDLIDKF
                     AGYGFNKSHAAAYALIAYQTAWLKAHHPVEFYAASMCFDIHQTDKLAVFVDDMRRMEV
                     ECLPPSINDSEAEFAVEEVTPQPFGLSLSKASPSSAGKPEKEGFDKLSPNGEGVEPQR
                     LAVRYALGALKGVGEKAMELLVEERRGNGRFKSLEDFAERVDPRLLNRRQIESLAGGG
                     AFDALDSNRAAVFAAAETILAHAASAADARESGQGGLFGGGDSNVVPIRMPVAATWTL
                     AQRMAAEKESFGFYFSAHPVDRYRHLAEAHGARTFAALASLPAPPEGGRTGAVMAGMV
                     EEARWRTSARGRRYLMATMSDSSGQFVATVFDDDVCVQVEAAAKSGGCALLNVELDRR
                     PGEEAPRVTIRSLQLFESLSKRSRLQLEVEVDEADAIDRLRSVVAGERGGQGELRLKV
                     RLDGGEALLVLGRDFQLDAELAARLERLPGVAAVRLAVAPQPRLALVS"
     gene            complement(5796..6749)
                     /locus_tag="VF547_12780"
     CDS             complement(5796..6749)
                     /locus_tag="VF547_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263737.1"
                     /translation="MNAKTRISLRTFLLAAAAASLLAGCDWMKKKDPPQAQSKAELAA
                     AQRERIRKACASEGTYDRLKELVFDEASRIRKSDPRNLDPLAAAAVVRMEDPVVKSRD
                     DSLNVTVCGGRFVLELPPGAENAFDGERRLTAEVEYAAQAALDGSGLVYQMDGAEPII
                     YRLAAFGLPGQPMPRIVAMPQPSAPPGVPPQVAVVEPEAPMPPARPVEPVRPAVEKRK
                     AAQAPPPTRVTARPSFNCRYAKTGTEKMICGSPRLAARDREMASLWYDLMARSDPATR
                     NHLRRSRDAFLGRRERCGSEGCVDSAYASRMAELRRIAQGD"
     gene            complement(6793..7416)
                     /gene="lepB"
                     /locus_tag="VF547_12785"
     CDS             complement(6793..7416)
                     /gene="lepB"
                     /locus_tag="VF547_12785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8263738.1"
                     /translation="MSPSLRRAAWGAAAAVGGTIAVLIVALLGLFAVGYLKNFHLPAE
                     AMAPTLEKGDRFVALMNGGRDLKRGDVIVLAVHDATYVKRVAALGGDTIGLENGVVSL
                     NGRIVPQRLLKEERRRGSGGAPVRRLAEQFPGEEAPHEILDSGPSVVDDVPEVRVPSG
                     FLFVLGDNRDVSADSRVSRDQMGVALLPVADVKGRALFCTMGCKLGR"
     gene            complement(7413..8084)
                     /locus_tag="VF547_12790"
     CDS             complement(7413..8084)
                     /locus_tag="VF547_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8263739.1"
                     /translation="MNEVLEVRGLKRSFRQGGVTIDVLRGIGLEVRSGEIVALLGPSG
                     SGKSTLLQAVGLLEGGFEGSIRIAGEEVAELSDGNRTRVRRDTLGFVYQFHHLLPDFT
                     ALENVVIPQLVKGAGREEAEERARTLLATLGLADRLTHRPSKLSGGEQQRVAVARALA
                     NRPALVLADEPTGNLDEATADIVFAEFLRLVRGEGTAALVATHNERIAAKMDRILRLH
                     EGVLE"
     gene            complement(8077..9327)
                     /locus_tag="VF547_12795"
     CDS             complement(8077..9327)
                     /locus_tag="VF547_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX8263740.1"
                     /translation="MILSRYERMIARRYLLPGKGEGFIFLVAAISLVAVALGVAALIV
                     VMSVMNGFRAELFDKIVGLNGHAVVQGYGGQLPDWREVVRQARATPGVTDALPLIEQP
                     LMVSHDGRVEGVLVRGMDRADILRNDTLQGKVLSGSLESLRPGVGNVAIGARLAEALG
                     ANVGGQISLISPDGRTTPFGTVPRIVSYNVSAIFEVGIYDYDKSFVIMPIEDAQTLLL
                     MGDAVGMVEVQTADAHRVGEILAPLRAQVSGRAAVADWRSMNQSLFEALQVERVAMFV
                     ILSIIILVAVFNILSSLIMLVRAKTRDIAILRTMGATRRGLMKVFVTVGVTIGVIGIA
                     AGLGLGALLLYFRQPFVNFFQFLTGQNLWDPSIRFLTELPSKTDPFEVLAIVTMALVL
                     SFLATLYPAWKAASTDPVQVLRYE"
     gene            complement(9434..10738)
                     /locus_tag="VF547_12800"
     CDS             complement(9434..10738)
                     /locus_tag="VF547_12800"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013934052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX8263741.1"
                     /translation="MRLSRYFLPVMKETPADAQIVSHRLMLRAGLVRQTAAGIYAWLP
                     LGLRVLRRIEGIVREEQDRAGAVELLMPTLQSADLWRQSGRYDAYGPEMLRIRDRHDR
                     DMLYGPTNEEMVTALFRDGARSYRDLPRILYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLDEAGLRSSYEAMFVAYLRTFARLGLTAVPVKAPTGPIGGDLSHEFHILADTGE
                     SALFYDAAIEDISRDELLAADSSTIARLTGLYAMEEEEHGKVADCPVPPDRLRTRRGI
                     EVGHIFAFGTKYSAAMGLSLQTREGEQVHPQMGSYGIGVSRLMGAIIEASHDDKGIVW
                     PDAVAPFEVGLVNMRSDDGNVTSAADEIYGRLQEAGVETLYDDRDERGGAKFATMDLI
                     GLPWQLIVGPKGLERGVVELKRRAGGEREELSIESALARLTA"
     gene            11440..11703
                     /gene="infA"
                     /locus_tag="VF547_12805"
     CDS             11440..11703
                     /gene="infA"
                     /locus_tag="VF547_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX8263742.1"
                     /translation="MAKEATLTVEGQIDEIYPDGRFGVMLDNQHRVIVYTAGKMRRFR
                     IRSVVGDRVHVEMTPYDLTKGRLIFRERTPGQGPGPRRPGAKR"
     gene            11714..11941
                     /locus_tag="VF547_12810"
     CDS             11714..11941
                     /locus_tag="VF547_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263743.1"
                     /translation="MGGTPDSNRAAIKPFLIAKDGDGKVRLTLRETRYNGQGYPIVTT
                     TVHDEPFATAAAARAYAKEHFGAEPGQFASK"
BASE COUNT         1830 a         4062 c         4206 g         1845 t
ORIGIN      
        1 aattcagctg tcgccgcccg acgggcgggt cgaggtcgaa gtccgttaca agaatttccc
       61 catacagcgg gaggtgattg cggacgatgt cgataacttc acgttcagct ttccagcgag
      121 ggggagattt acggtgggaa ggaacatcat aggcataatt ctgagtgtgg ccgtagtcct
      181 ggggatagtt ttggtggcat tctatctcgg ggcgcaaggg aaggttttgc ctctcgctct
      241 aggggtggtg ctggtggcca cggccttgac gctggccttt gccttcaagg atgccaatcc
      301 gcttcagcag cagttggtgc ggtcgacctt tgctattgga gtcggagcct tggcctccca
      361 agtgcccggg cttctgaaca taaatgtcac gatcggcacg acggcgacga tcagcgccgc
      421 cggagcgatc gcggcgttcg tggtgacctt cttcttcgtg ccggcaggcc agccgcagcc
      481 ctgacccgag acgaagaccc cggggcgacg ggtcaggcgg cgctggcgag cttgtagtcg
      541 cgatactgct ccctcagcgc cgtcttcaga agcttgcccg tcgcggtgtg gggcaggctg
      601 tcgacgaaca atatctcgtc cggcagccac catttggcga catggccgga aaggtggtcg
      661 aggatctgct gcggagtcac ttcggcgccg gccttgcgca ccaccagcag cagcggccgc
      721 tcgtcccatt tggggtgctt gaccccgacc gccgccgcct cggccacgcc cgggcagccg
      781 acggcggcgt tctcgaggtc gatcgagctg atccattcgc cgccggactt gatcacgtcc
      841 ttggaacggt cggtgatctg cagcactccg tcgggatgga gcgcggcgac gtcgcccgtg
      901 tcgaaccagc catgctcgtc gatgacgtcg ccggccggat cctggaaata ctggcggatg
      961 atccagggcc cccgggtctg gaggcggccg gacgtcctgc cgtcgcgggg aaggacgttg
     1021 cccgcctcgt cgacggtgcg cagctcgacc ccgaacggca cccggccctg cttgcacagc
     1081 agggcgaccc gctgctcgcg gctgagctca tcccagtcgc cgctgcccgc gccgacggtg
     1141 ccgagcggcg acatctcggt catgccccag gcgtggccga cccggatgcc gcggtccatg
     1201 aaccattcga tcatcgcccg cggcgcagcc gagccgccga tatggacggt ggtgagcttg
     1261 cccaggtcct cgccggtcga gtcgacgtgg ttgacgaggg cgagccagac cgtcggcact
     1321 ccggccgtgt gggtcacgcc ctcggccttc atcaggtcgc acatggcctg gggcgtgtag
     1381 tcggtcgaga agaccatctt gccgcccgcc atcgcggtgc agaagggcaa ggcccagccg
     1441 gcggcgtgaa acatcggcac caccggaagc accaccgccc gggcggagag gtcgaagacg
     1501 tcggggctca tctgcaccat ggcgtggagc acggtcgagc gatgctcgta gagcacgccc
     1561 ttcgggttgc cggtggtgcc gctggtgtag cagagcatcg ccgggtcgcg ttcgtcgccc
     1621 tcgacccagg cgtaatcgtc gccctcggcg gcgagcaggt cgtcgaactc gccattttcg
     1681 aagcagatga aacgctcgat gctggtccag cgcggcttca gccgctcgac gatcggcgcg
     1741 aacgcgcggt cgtagaacag cacccggtcc tcggcatggt tggcgatgta gacgagctgg
     1801 tcgtcgaaca ggcgcggatt gatggtgtgg atgatgccgc ccatgccgat cgtcccgtac
     1861 caggcgacga cgtggcggct gtggttcatc gccagcgtgc cgacccggtc gcccggctcg
     1921 atccccagcc gctccagcgc cagagcgagc cgtcgcgagt cccgggcgat cccggcccag
     1981 tcggtgcgca cggtggtgcc gtcggcgtag agggagacga gctcgcgcgc gccgtgctcg
     2041 cgcgccgcat ggtcgaggat ccgcatgatc ctcagcggcc agtcctgcat cccgcccagc
     2101 atgagcccct cctcggtcag gagcgcagtt tatgcgctca ggacacgagc gcaagccggg
     2161 gctggggcgc caccgccagc cgaaccgcgg cgacgccggg gagccgctcg aggcgggcgg
     2221 cgagctcggc gtcgagctgg aagtcgcggc cgagcacgag cagcgcctcg ccgccgtcga
     2281 gccggacctt gaggcgaagc tcgccctggc cgccgcgctc gcccgccacg accgagcgga
     2341 ggcgatcgat cgcgtccgct tcgtcgacct cgacctcgag ctggagccgg ctgcgcttcg
     2401 acaggctctc gaacaattgc agcgagcgga tggtgactcg cggcgcctcc tcgcccggcc
     2461 gccggtcgag ctcgacgttg agcagggcgc agccgcccga cttggccgcg gcctcgacct
     2521 ggacgcagac gtcgtcgtcg aacacggtgg cgacgaactg gccggacgaa tcggacatcg
     2581 tcgccatcag gtagcgccgg ccgcgggcgg aggtccgcca gcgcgcctcc tccaccatcc
     2641 cggccatgac cgcgccggta cgaccgcctt ccgggggcgc gggcagcgag gccagagcgg
     2701 cgaaggtccg ggcgccgtgc gcctcggcga ggtggcggta gcggtcgacc ggatgggcgg
     2761 agaagtagaa gccgaacgac tccttctcgg cggccatgcg ctgcgccagc gtccaggtcg
     2821 cggcgaccgg catgcggatc ggcacgacgt tcgaatcgcc gccgccgaac aggcctccct
     2881 gcccgctctc gcgcgcgtcg gcggcgctgg cggcgtgggc gaggatggtc tcggcggcgg
     2941 cgaagaccgc ggcgcggttc gaatcgagcg cgtcgaaggc gccgccgccg gcgaggctct
     3001 cgatctggcg gcggttgagg aggcgcggat ccacccgttc ggcgaagtcc tcgagcgact
     3061 tgaagcggcc gttgccgcgc cgctcctcga ccagcagctc catcgccttc tcgccgacgc
     3121 ccttgagggc gcccagcgcg tagcggacgg cgagacgttg cggctcgacc ccttcaccgt
     3181 tcgggctgag cttgtcgaag ccctcttttt ccggcttgcc cgcggaagaa ggagaagcct
     3241 tcgacaggct caggccgaac ggctggggag tgacctcctc gaccgcgaac tcggcctcgc
     3301 tgtcgttgat cgagggcggc aggcactcga cctccatccg ccgcatgtcg tcgacgaaga
     3361 cggccagctt gtcggtctgg tggatgtcga agcacatcga ggcggcgtag aactcgaccg
     3421 gatgatgcgc cttcagccag gccgtctggt aggcgatcag ggcgtaggcg gcggcgtggc
     3481 tcttgttgaa gccgtagccg gcgaacttgt cgatcaagtc gaacagctcg ttggccttgg
     3541 ccttggggat cgagttgacc cgctcgcagc cggcgacgaa gccggcgcgc tgggcgtcca
     3601 tctcggactt gatcttcttg cccatcgccc gccgcagcag gtcggcctcg ccgaggctga
     3661 agccggccag caccttggcc gcctccatca cctgctcctg gtagacgaag atgccgtagg
     3721 tctccttcag caccggctcg agcaggggat gcggataggc gatcggctcg cggccgttct
     3781 tcctcgcgcc gaaggtcggg atattgtcca tcggccccgg ccgatagagc gagaccagcg
     3841 cgatgatgtc ctcgaacgtg gtcggcttca ccgccgccag agtgcgccgc atgccctccg
     3901 attcgagctg gaagacgccg accgtgtcgc cgtgctgaag caattcgtag actaggggat
     3961 cgtcccaggc gaggcggtcg agatcggcct cgacgccccg cttcgccagc atctgcaccg
     4021 ccttctgaag caccgacaga gtcttgaggc cgaggaagtc gaacttcacc aggcccgccg
     4081 cctcgacata tttcatgtcg aactgggtga ccggcatgtc cgagcgtggg tcgcggtaga
     4141 gcggcaccag cttgtcgagc ggccggtcgc cgatgaccac gccggcggca tgggtggagg
     4201 cgtggcgcgg caggccctcg agcttcatcg cgaggtcgaa caggcggcgg acctgcgcgt
     4261 cgttcttata ttcggcggcg agctcggaga ctccgttcag cgcgcgatcg agcgtccagg
     4321 ggtcggtcgg gtggttgggg atcgccttgg cgagccggtc gacctggccg tagggcatct
     4381 ggaggacgcg gccggtgtcc ttgaggacgg cgcgggcctt cagcttgccg aaggtgatga
     4441 tctgggcgac cttgtcgctg ccgtagcgct gctggacgta gcggatgacc tcgccgcgcc
     4501 tcgtctcgca aaagtcgatg tcgaagtccg gcatcgacac gcgctccggg ttgaggaagc
     4561 gctcgaacag caggccgagc cggatcggat cgagatcggt gatggtgagc gcccaggcga
     4621 cgacggagcc ggcgccggag ccgcggcccg gccccaccgg tatgtcgttc tccttggccc
     4681 atttgatgaa gtcggcgacg atcaggaaat aggccgaaaa gcccatccgg acgatgatgt
     4741 ccgcctcata gtcgaggcgg tcgaaatagg cttggcgctc ctcccccgtc agctcgccgc
     4801 gatcggcgag ccgcgcctcc aggcccgcac gggcgtcgcg gcggagctgg tcggcctcgg
     4861 cctcgacgtc gccggcgatg cttgggagga tcggcctgcg ctgcggcgcg ccgaacgcgc
     4921 agcggcgcgc gacgactccg ctattctcga tcgcttcggg caagtcggcg aacagccggc
     4981 gcatctccgc ggccggcttc agccaggttt cggatgagga tctccggcgg tcgtcgcggt
     5041 cgagctggct cgactgggcg atgcacagca aggcgtcatg cgcctcgtgg aaatgggcgt
     5101 cggcataggc tgcgggattg gtcgcgacca gcggcagatc gtgccgatag gcgagctcga
     5161 cgaggcgggc ttccgagcgc tgctcgccct cgtcctggcg gcgcgagatc tcgatgtaga
     5221 gccgccccgg gaaaagcgac atgaggcgtt cgaggatgag gtcggcggcg gcgtcctggc
     5281 cctggctgat cagcctcacc agcgcgccgt cggcgccggc ggtcagcgcg atcagcccgt
     5341 cggttcggcc gtccagcgcg tcgaggctga catgggccgg ctcctcgacc gggcggccga
     5401 gatgggcggc ggagacgagc gtgcacagat tgtcgtagcc gcggtcgttc tgcgcgtaga
     5461 gcgcgatcca gtcgagcgcc ggcggccctc cggcgcaatc cggccgggcc accgcgagca
     5521 tcgttccgac gatcggctgc accccggcct cgcggcaggc gtcggtgaac gccatcgccg
     5581 cgtagaggcc gttgcggtcg gtcagcgccg cggccgggaa atcgagccgt ttcgccagct
     5641 tggcgatcgc cttcggatcg atcgcccctt cgagcatcgt gtagctcgag aagatgcgca
     5701 gtgggacgta ggggccggcc atggcgtcac tatggtggct gcggcgatcc gatggaagcg
     5761 ccgcccgccg ggttttccac acctaatcgt cccggtcagt cgccctgcgc tatccgtctc
     5821 agctcggcca tgcgcgaggc gtaggcggag tcgacgcagc cctcgcttcc gcagcgttcg
     5881 cgccggccga ggaaagcatc gcggctgcgc ctcaaatgat tgcgggtggc ggggtccgag
     5941 cgggccatca aatcgtacca gagggaggcc atctcgcggt cccgggccgc gaggcggggc
     6001 gagccgcaga tcatcttctc ggtgccggtc ttcgcgtagc ggcagttgaa gctcggccgc
     6061 gccgtcaccc gagtcggcgg aggcgcttgg gcggccttgc gcttttccac ggccggccgg
     6121 accggttcca ccggccttgc cggcggcatc ggcgcttcgg gctccacgac cgcgacctgg
     6181 ggcggcacgc ccggcggcgc gctcggctgg ggcatcgcga cgatcctggg catcggctgg
     6241 cccggcagcc cgaaggcggc gaggcgatag atgatcggtt cggccccatc catctggtag
     6301 acgaggccgc tgccgtcgag cgcggcctgc gcggcatatt cgacctcggc ggtcagtcgg
     6361 cgctcgccgt cgaacgcatt ctcggctccg ggcggcagct cgagcacgaa acggccgccg
     6421 cagacggtga cgttgagcga gtcatcccgg ctcttgacca ccggatcctc cattcggacc
     6481 acggcggccg cggcgagcgg atcgaggttg cgggggtcgg acttgcggat ccgcgacgcc
     6541 tcgtcgaaga cgagctcctt gagccggtcg tacgtccctt ccgaagcgca ggccttgcgg
     6601 atccgctccc gctgcgcggc ggcgagctcc gccttgctct gcgcctgcgg cggatccttc
     6661 ttcttcatcc agtcgcagcc ggcgagcagc gacgcggcgg cggccgcgag cagaaacgtc
     6721 ctcagactga tgcgggtctt cgcgttcatc cggcgccaac gcagcagcgg cggcatctat
     6781 cccccgcacc gctcagcggc cgagcttgca gcccatcgtg cagaagaggg ctcgcccctt
     6841 gacgtccgcg accggcagca gcgcgacgcc catctggtcc cgcgagaccc ggctgtcggc
     6901 ggagacgtcg cgattgtcgc cgaggacgaa gaggaagccg gacgggaccc gaacttcggg
     6961 aacgtcgtcg accacggacg gccccgaatc caatatctcg tgaggcgcct cctcgccggg
     7021 gaattgctcg gccaggcgcc ggacgggcgc gcccccgctt ccccgccggc gctcctcctt
     7081 cagcagtcgc tgagggacga tgcggccgtt gaggctgacg accccgttct cgaggccgat
     7141 cgtgtcgccg cccagcgcgg cgacccgctt gacgtacgtc gcatcgtgca ccgccaggac
     7201 gatgacgtcg ccgcgcttga ggtcgcggcc gccgttcatc agcgcgacga accggtctcc
     7261 cttttcgagg gtcggggcca tcgcctccgc cgggaggtgg aaattcttga ggtagccgac
     7321 cgcgaagagt ccgagcagcg ccacgatcag cacggcgatc gttccgccga ccgcggccgc
     7381 cgctccccac gcggcgcggc ggagcgaagg gctcattcca gcacgccttc gtgcagcctg
     7441 aggattcggt ccatcttggc ggcgatccgc tcattatggg tggccaccag ggcggcggtg
     7501 ccctcgccgc ggacgagtcg gaggaactcg gcgaagacga tgtcggcggt ggcctcgtcg
     7561 agattgccgg tgggctcgtc ggccagcacc agcgccggtc ggttggccag cgcccgggcg
     7621 acggcgaccc gctgctgctc gccgcccgac aatttgctcg gccggtgggt cagccgatcg
     7681 gccaggccga gcgtggccag cagggtccgc gcccgctcct cggcctcctc ccggccggcc
     7741 cccttgacca gctgcgggat gacaacgttc tcgagcgcgg tgaagtcggg gagcaggtgg
     7801 tggaactggt agacgaagcc gagcgtgtcg cggcgcacgc gggtgcggtt gccgtcgctg
     7861 agctcggcca cctcctcgcc cgcgattcgg atcgagcctt cgaacccgcc ctcgagcagc
     7921 cccacggcct gcagcagggt ggacttgccc gagcccgagg ggccgagcag cgcgacgatc
     7981 tcgccgcttc gcacctcgag gcctatgccg cgcagcacgt cgatggtgac gcccccctgc
     8041 cggaagctgc gcttcaggcc gcggacctcg agcacctcat tcatagcgga gcacctgcac
     8101 cggatcggtg ctcgccgcct tccacgccgg gtagagcgtc gcgaggaagc tcagcaccag
     8161 ggccatggtg acgatcgcca gcacctcgaa cgggtcggtc ttggacggca gctcggtgag
     8221 gaatcggatc gaggggtccc acaggttctg gccggtcagg aactggaaga aattgacgaa
     8281 gggctggcgg aagtagagca gcagcgcgcc gaggccgagg ccggcggcga tgccgatcac
     8341 tccgatcgtg acgccgacgg tgacgaacac cttcatcagc ccgcgccggg tcgcgcccat
     8401 ggtccgcagg atggcgatgt cgcgggtctt ggcgcgcacc agcatgatca gcgaggaaag
     8461 gatgttgaac accgcgacca ggatgatgat cgacaggatg acgaacatcg ccacccgctc
     8521 cacctgcagc gcctcgaaca gcgactggtt catcgagcgc cagtcggcga ccgccgcccg
     8581 gccgctgacc tgggctcgaa gcggcgccag gatctcgccg acgcggtggg cgtcggccgt
     8641 ctggacctcg accattccga ccgcgtcgcc catcagcagc agggtctgcg cgtcctctat
     8701 cggcatgatc acgaacgact tgtcgtaatc gtagattccc acctcgaaga tcgcggagac
     8761 gttgtaggag acgattcggg gcaccgttcc gaacggggtc gtccgaccgt cggggctgat
     8821 caggctgatc tggccgccga cattggcccc gagcgcctcg gcgaggcggg cgccgatcgc
     8881 gacgttgccg accccgggcc gaagcgactc cagcgagccc gagaggacct tgccctgcag
     8941 ggtgtcgttg cgcaggatgt cggcgcggtc catgccgcgg accagcacgc cttccaccct
     9001 tccgtcgtgg ctcaccatca acggctgctc gatgagcggc aaggcgtcgg tgactcccgg
     9061 cgtggcgcgc gcctgcctga cgacctcgcg ccaatcggga agctggccgc catagccctg
     9121 aaccacggcg tggccgttca gcccgacgat cttgtcgaac agctcggccc tgaagccgtt
     9181 catcacgctc atcacgacga tcagggccgc gactccgagc gcgaccgcga ccaggctgat
     9241 cgccgcgacg aggaagatga acccctcgcc cttccccggc agcaggtagc gccgggcgat
     9301 catccgctcg tagcgggaca ggatcatgac ggctccccca aaccgtcatt gcgagcgaag
     9361 cgaagccatc cagaacggcg catgctcccg ctggattgcc gcgtcgcttc gctgctcgca
     9421 atgacggcgt ccattacgcc gtcagcctcg ccagggcgct ctcgatcgac agctcctcgc
     9481 gctcgccgcc ggcgcggcgc ttgagctcga cgacgccgcg ctccagcccc ttgggcccga
     9541 cgatcagctg ccaggggagc ccgatcaggt ccatggtggc gaacttggcg ccgccgcgct
     9601 cgtcgcggtc gtcgtagaga gtctcgactc cggcctcctg gagccggccg tagatctcgt
     9661 cggcggcgct ggtgacgttg ccgtcgtcgg agcgcatgtt gaccagcccg acctcgaacg
     9721 gggccacggc gtcgggccac acgatgcctt tgtcgtcgtg gctggcctcg atgatcgccc
     9781 ccatcagacg cgacacgccg atgccgtagc tgcccatctg cgggtggacc tgctcgccct
     9841 cgcgggtctg gaggctgagg cccatcgcgg cgctgtactt ggtgccgaag gcgaagatgt
     9901 ggccgacctc gatgccgcgg cgggttcgga gccggtcggg cggcaccggg cagtcggcga
     9961 ccttgccatg ctcctcctcc tccatcgcgt agagcccggt gaggcgggcg atggtcgagg
    10021 agtcggcggc gagcagctcg tcgcgcgaga tgtcctcgat cgcggcgtcg tagaacagcg
    10081 cgctctcgcc ggtgtcggcg aggatgtgga attcgtggct gaggtcgccg ccgatcgggc
    10141 ccgtcggcgc cttgaccgga accgcggtga ggccgaggcg ggcgaaggtc ctgaggtagg
    10201 cgacgaacat cgcctcgtag ctggagcgga gccccgcctc gtcgaggtcg aagctgtagg
    10261 cgtccttcat caggaactcg cggccgcgca tcactccgaa gcgggggcgg acctcgtcgc
    10321 ggaacttcca ctggatatgg tagaggatgc ggggaaggtc gcgatagctc ctggcgccgt
    10381 cgcggaagag ggcggtgacc atctcctcgt tggtgggccc gtagagcatg tcgcggtcgt
    10441 ggcggtcgcg gatccggagc atctcggggc cgtaggcgtc gtagcggccg gactggcgcc
    10501 agaggtcggc cgattgcagc gtcggcatca gcagctcgac cgctccggcg cggtcctgct
    10561 cctcgcggac gatgccctcg atccgcctga gcaccctcag tccgaggggg agccaggcgt
    10621 agattccggc ggcggtctgg cggacgaggc cggcgcgcag catcagccgg tggctgacga
    10681 tctgggcgtc ggcgggagtc tccttcatga ccgggaggaa gtaacgggac agtcgcatgg
    10741 caagcgcctc tacgggccgc cccccgccaa ggcaaccgcg ccggcctgtt gccctgccga
    10801 cacaggccct gagcaaagtg cggcaagccc ctggataggc agtgacaaga cgccgagtcg
    10861 ctcatagcgt cccggccacg aacgaaggcg acagccgggt tcggggaggc cttgtgcccc
    10921 gcatcctcat gcgagggagg atgtgagcgg gcgggggcat cttacaaggg ggtcggcgtg
    10981 caagcgccgc gacggtgccc gggcagcgat gtccgggcac tttcaatttg ccccgcgccg
    11041 aggaggatgt tggcaaaggt gccgcgcagc gtgacggggg cgccgggcaa tggcggaaat
    11101 ccgccgttcc cgcgcccgca atcgccagaa aacgcgcagg gaccgccatt cggcgcaggc
    11161 gcttgggcgg cgcgcggggg cagaaggacg agaacgcccc ctcctcgctc gacgctccag
    11221 ctgggggccg cgacgcacgg gacgcccagc cgcgccgcgg gcccgacatt gctaaaaccg
    11281 cccccagcgc ctatctagag cgggctaacg tcgcctgcga cggatttcgg cgccctccag
    11341 cggccccgcc ttccctttca agcttctcgc ccacgcctgc ggcctgcgcc gcccgcgcgg
    11401 ccatccaatg ccggccgcgc gccgagggag atacgaagca tggcgaagga agcgactctc
    11461 accgtcgagg gccagatcga cgagatctat cccgacgggc ggttcggggt gatgctggac
    11521 aaccagcacc gggtgatcgt ctacaccgcc ggcaagatgc ggcgcttccg gatccgctcg
    11581 gtggtcggcg accgggtcca tgtcgagatg accccctacg acctcaccaa gggccggctg
    11641 atcttccgcg agcgcacgcc cggccagggc cccggcccgc gccggcccgg cgccaagcgg
    11701 taggagacga gacatgggcg gaacccccga cagcaatcgc gccgcgatca agcccttcct
    11761 gatcgccaag gacggcgacg gcaaggtccg tctcaccctg cgcgagaccc gctacaacgg
    11821 ccagggctat ccgatcgtca cgacgaccgt gcacgacgag ccgttcgcca ccgcggccgc
    11881 ggcgcgcgcc tatgccaagg agcatttcgg cgccgagccc gggcagttcg cgagcaagtg
    11941 acg
//
LOCUS       DASVQZ010000455         6252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_958314, whole genome shotgun sequence.
ACCESSION   DASVQZ010000455 DASVQZ010000000
VERSION     DASVQZ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958314"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1490
                     /gene="prsR"
                     /locus_tag="VF547_12815"
     CDS             126..1490
                     /gene="prsR"
                     /locus_tag="VF547_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX8263744.1"
                     /translation="MSVDTRAKLLIVEDDEGLQRQLRWAYDDYDVLVASNRDAAINLL
                     RSEEPPVVTLDLGLPPDPNGVTEGFATLETILSLKPETKVIVASGHGARESALRAIAS
                     GAYDFYQKPVDIDQLGLIVRRAFQLHGIETENRKLEERVGEDQTVLGTMITGAPEMLK
                     VARTIERVANTGVSVMLLGASGTGKELLARGLHSASNRAGGAFVAINCAAIPENLLEA
                     ELFGYEKGAFTGAIKTTEGKIELAHGGTLFLDEVGDIPLPLQVKLLRFIQERVIERIG
                     GRKPIAVDTRIVSATHQDLPEMIKAGSFREDLYYRLAEIVVRIPSLSERHGDSVLLAK
                     HFLHRFAREMNPQVKGLSASALAAIDEWPWPGNVRELENRMKRAVIMADGKLVAAEDL
                     DLDAPAEAALPVNLKAARELADRRAIRQALARTENNISGAAKLLGISRPTLYDLMKQY
                     DLQP"
     gene            1487..3409
                     /locus_tag="VF547_12820"
     CDS             1487..3409
                     /locus_tag="VF547_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8263745.1"
                     /translation="MSARAALIALTLLGAAACSNVASSPDEAYRRGLQALADGQPRTA
                     RIEFLNAIKADPDNKALRVAQARTYLLLGDGAAAEDEIKRANALGVPETETAHLMAHA
                     LLLQGQAERAVAEAGKAGPAHAAYAQRMLGRAQLALGRLEEAARAFDRALAAAPADGA
                     LWADIGRFRRDTGEVAGAIAAADRAVALDPRNVEALVLRGELTRNQYGLAAAMPWFDR
                     ALEIDPSHVPALIERAATLGDLGRTREMLADTRRILAVSPGNPSGYYYQAVLAARGGD
                     YELARSLYRRTNGAFDKRPAAMLLAGTIELGAGNAALATRPLKRLLDAQPGNLKARRL
                     LGSAQWRSGDSSGAVRTLRPIADRPDADAYTLSVIGKAYARLGDEALAARYLARAAAP
                     RPSSLAALENPLRDQEIEALRREADRDPRSAPAQVALIRALLGRGLGPEALQRARRLQ
                     AANKGAPDAHVLVGDALAIQGDYAAAASAYRMAANLAFSEPVAMRLVEALRNSGDERG
                     ASQALALFLQQNPQNVAAQIVAANANLQARRWPEAIALYERARKRVGDRDATLLNNLA
                     WAYAETGDYGRALPLARRAWALEPRNPATADTLGWLLFKSGEDRVGGLALLEQAVRGA
                     PSDAEIRAHLKDARGG"
     gene            complement(3538..4152)
                     /locus_tag="VF547_12825"
     CDS             complement(3538..4152)
                     /locus_tag="VF547_12825"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX8263746.1"
                     /translation="MDSTDAADLIAEIHEDRAENAAAETFRNRAALLIALLAAVLAIG
                     GLGGGNATDDMIASNIKASDTWAFYQAKNVRQTMYEIEAEKLKAQLAAGTVPAAERPA
                     AEARLKDYEATIARYDSEPDPKDPTKGEGKKELSAQARAHETAFEQASERDNNFDYAE
                     VLLQLALVLGSVAILAVNRWILGLSLGLGVLGSLLTLNGFFLLV"
     gene            4284..5924
                     /locus_tag="VF547_12830"
     CDS             4284..5924
                     /locus_tag="VF547_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8263747.1"
                     /translation="MKLARLAALLSLPLAAAAAAAPPDKPKLVVVISVDQFSADLFAQ
                     YRARFDGGLERLSQGVVFPAGYQGHNATETCPGHSTILTGSRPARTGIIANNWFNLGL
                     AREDKGVYCSEDPRVPGSSSKGGQYTVSSYHLRVPALGDRMKRADPGSRVAVVSGKDR
                     AAIMMGGYGPDQRWWWDHDAKAFVNPGAPAAPRSVAEANQAVTEALSRPRPARDLPDV
                     CEAHSRAVPVTGGGKPVGAWRFDRAAGDKNLFRASPDFDRATLDLGEALRREMKLGEG
                     ESTDLLILGLSATDYIGHSFGTQGSEMCIQLLALDEALGAFFRNLDSTGIDYLVTLTA
                     DHGGVDIPERARAQASGDAMRVDPALNAGNMAKALAQRLKLTGDLLYGDGAFGDMYVD
                     RRLTAAQRARVLREAVAAYRRHPQVAAVFTKAELAAAAGPSGPPDSWSLLQRAKASFD
                     SERSGDFVVLLKPRVTPIFDTSRGYVATHGSPWDYDRRVPILFWRKGMARFEQPLAVE
                     TADILPTLAAVLAVPIAPGTIDGRCLDLDAGPGNSCVR"
     gene            complement(5979..>6252)
                     /locus_tag="VF547_12835"
     CDS             complement(5979..>6252)
                     /locus_tag="VF547_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HEX8263748.1"
                     /translation="AAKPAAAPARPQTKLSYKDQRDYDLLPDRIQALEAQIARDEEAL
                     HDPDLYARDPRRFAALTAAIEAARAERDAAEHRWLELAEKVEALAG"
BASE COUNT          918 a         2262 c         2185 g          887 t
ORIGIN      
        1 cgaagcctcg tcgcggccat gggcggccgc atcgaggtga gcagccaggt cggccagggc
       61 agtcgattca ccatcctttt accgctgggt gagaccagct tcagccaagt cgatcggaaa
      121 gtcgcatgag cgtggacacc agggcgaaac tgctgatcgt cgaggacgat gaggggctgc
      181 agcgccagtt gcgctgggcc tatgacgatt acgacgtgct ggtcgcctcc aaccgcgacg
      241 cagcgatcaa cctgttgcgc tcggaggagc cgccggtggt cacgctcgac ctcggcctgc
      301 cgcccgaccc caacggagtc acggagggct tcgcgacgct ggagacgatt ctgtcgctga
      361 agcccgagac caaggtcatc gtcgcttccg gccacggagc gcgcgagagc gccctgaggg
      421 cgatcgcgtc cggcgcctat gatttctacc agaagccggt cgacatcgac cagctcgggc
      481 tgatcgtgcg ccgcgccttc cagctgcacg gcatcgagac cgagaaccgc aagctcgagg
      541 aacgcgtcgg cgaggaccag acggtgcttg ggacgatgat caccggcgct cccgagatgc
      601 tcaaggtcgc tcggacgatc gagcgcgtcg cgaacaccgg cgtctccgtg atgctgctcg
      661 gcgccagcgg caccggcaag gaattgctcg cgcgcggcct gcacagcgcc agcaaccggg
      721 ccggcggggc cttcgtcgcg atcaactgcg ccgccatccc cgagaatctg ctcgaggccg
      781 aattgttcgg ctacgagaag ggggcgttca ccggcgcgat caagaccacc gagggcaaga
      841 tcgagctcgc ccatggcggc accttgttcc tggatgaggt cggcgacatc ccgctgcccc
      901 tccaggtcaa gctgctgcgg ttcatccagg agagggtgat cgagcggatc ggcggccgca
      961 agccgatcgc ggtcgacacc cgaatcgtca gcgcgaccca ccaggacctg cccgagatga
     1021 tcaaggcggg cagcttccgc gaggacctct attaccgcct cgccgagatc gtcgtgcgca
     1081 tccccagcct cagcgagcgc cacggcgact cggtgctgct cgccaagcat ttcctgcacc
     1141 gcttcgcccg cgagatgaac cctcaggtca agggcctcag cgcgagcgct ctggcggcga
     1201 tcgacgaatg gccctggccg ggcaacgtcc gcgagctcga gaaccggatg aagcgggcgg
     1261 tgatcatggc cgacggcaag ctggtggcgg ccgaggatct cgacctcgac gcgccggccg
     1321 aggcggcctt gccggtcaac ctcaaggccg cgcgcgagct cgccgaccgc cgggcgatcc
     1381 gccaggcctt ggcgcggacc gagaacaaca tctcgggcgc cgcaaagctg ctcgggatca
     1441 gccggccgac gctctacgac ctcatgaagc aatacgatct ccagccgtga gcgcgcgggc
     1501 cgccctgatc gcgctgaccc tgctcggcgc cgccgcctgc tcgaacgtgg cgtcgtcgcc
     1561 cgacgaggcc tatcgccgcg ggcttcaggc gctggcggac gggcagccgc gcaccgcccg
     1621 gatcgagttc ctgaacgcga tcaaggccga tccggacaat aaggcgctgc gagtcgccca
     1681 ggcgcggacc tacctgctgc tgggcgacgg cgccgccgcc gaggacgaga tcaagcgggc
     1741 caacgcgctc ggagtgcccg aaaccgagac cgcgcacctg atggcccatg cgctgctgct
     1801 ccaggggcag gccgagcgcg ccgtcgcgga ggccggcaag gccggccccg cccacgcggc
     1861 ctatgcccag cgcatgctcg gccgcgccca gctggcgctg ggccggctgg aggaggcggc
     1921 cagggccttc gaccgggcgc tggcggcagc gcccgcggac ggcgcgctgt gggccgacat
     1981 cggccgcttc cgccgcgaca ccggcgaggt ggccggtgcg atcgccgccg ccgaccgcgc
     2041 ggtcgccctc gacccccgca atgtcgaggc gctggtgctt cgcggcgagc tcacccgcaa
     2101 ccaatacggc ctcgccgccg ccatgccctg gttcgaccgc gcgctggaga tcgatccgag
     2161 ccacgtcccg gcgctgatcg agcgcgccgc cacgctcggc gacctcggcc gcacccgcga
     2221 gatgcttgcc gacacccgcc ggatcctcgc cgtttcgccc ggcaacccct cgggctatta
     2281 ctaccaggcg gtgcttgccg cgcgcggcgg cgactacgag ctggcccgga gcctctatcg
     2341 ccgaaccaac ggagcgttcg acaagcgccc ggccgcgatg ctgctggcgg ggacgatcga
     2401 gctcggcgcc ggcaacgccg cgctcgcgac ccggccgctg aagcggctgc tcgacgctca
     2461 gccgggcaat ctcaaggcgc gccgcctgct cggctcggcc cagtggcgca gcggtgactc
     2521 gtccggggcg gtgcggaccc tgcgtccgat cgccgaccgg ccggacgccg acgcctatac
     2581 cctgagcgtg atcggcaagg cctatgcgcg gctcggcgac gaagcgctcg ccgcccgcta
     2641 tctcgcgcgc gccgcggcgc cccgcccatc gtccctcgcc gcgctcgaaa acccgctccg
     2701 ggaccaggag atcgaggcgt tgcgccgcga ggccgaccgc gacccccgtt cggcgcccgc
     2761 gcaggtcgcg ctgatccgcg ccctgctcgg ccgcggcctc gggccggagg ctctgcagcg
     2821 ggcgcgtcgg ctgcaggcgg ccaacaaggg cgcgcccgac gcccatgtgc tggtcggcga
     2881 cgctctcgcc atccagggcg attatgcggc cgccgcgtcg gcctatcgga tggcggcgaa
     2941 tctcgccttc agcgagccgg tcgcgatgcg gctggtcgag gcgctccgca attccggcga
     3001 cgagcgcgga gcctcgcagg cgctggccct gttcctccag cagaatccgc agaacgtcgc
     3061 cgcccagatc gtcgcggcga acgccaatct gcaggcccgg cgctggccgg aggcgatcgc
     3121 gctctacgag cgggcgcgca agcgcgtcgg cgaccgcgac gccaccctgc tcaacaacct
     3181 cgcctgggcc tatgccgaga cgggcgacta cggtcgcgcc ctcccactcg cccgccgcgc
     3241 ctgggcgctc gagccgcgca atccggccac cgccgacaca ttgggctggc ttctgttcaa
     3301 gagcggcgag gaccgcgtcg gcggcctcgc cttgctcgag caggcggtcc gcggagcgcc
     3361 cagcgacgcg gagataaggg cgcatcttaa ggacgcgcga ggggggtgaa aattcctccc
     3421 cggcatttgc cggggagggg gaccaggcga agcctggtgg aggggtcctc ggtcgccgca
     3481 gagcccctcc accgcggtac gcgcggtccc cctcccccgg aaatccgggg aggaatttca
     3541 gaccagcagg aagaagccgt tcagggtcag gagcgacccc aggacgccga gccccagcga
     3601 caggccgagg atccagcggt tgacggcgag gatggcgacg gagccgagta cgagggcgag
     3661 ctggagcagg acctcggcat aatcgaaatt attgtcccgc tcggacgctt gttcgaacgc
     3721 cgtctcgtgg gcgcgggcct gggcggaaag ctccttcttg ccttcgccct tcgtcggatc
     3781 cttcgggtcc ggctcactgt cgtagcgggc gatggtcgcc tcatagtcct tgagccgcgc
     3841 ctcggccgcc ggccgctcgg cggcgggcac ggtgccggcg gcgagctggg ccttcagctt
     3901 ctccgcctcg atctcgtaca tggtctggcg gacgttcttg gcctgataga aggcccaggt
     3961 gtcgctcgcc ttgatgttgc tcgcgatcat gtcgtcggtg gcattgccgc cgccgaggcc
     4021 gccgatggcg agcaccgccg ccagcagggc aatcagcagg gcggcgcggt tgcggaaggt
     4081 ctcggcggcg gcgttctccg ccctgtcctc gtgaatttcg gcaatcagat cagcggcgtc
     4141 ggtcgaatcc atggcgtgtc ggggccccct ttcgcgcttc ggctcgcaaa ttttcgacgg
     4201 caagtcgagc ggcttcatgg cccttgcccg aagcccgtgc cggtgtagat gcggaggtcg
     4261 agaacgataa tggggaaacg ccgatgaaac tcgcccgcct cgccgccctg ctctctttgc
     4321 cgcttgccgc cgcggccgcc gcggcgccgc ccgacaagcc gaagctggtg gtggtgatct
     4381 cggtcgacca attctccgcc gatctgttcg cccaataccg ggcccggttc gacggcggcc
     4441 tcgagcggtt gtcccaaggc gtggtcttcc cggccggcta tcagggccac aacgccaccg
     4501 agacctgccc aggccattcg accatcctca ccggctcgcg gccggcgcgg acgggcatca
     4561 tcgccaacaa ctggttcaac ctcggcctcg cccgcgagga caagggggtc tattgctccg
     4621 aggatccgcg ggtccccggc agcagctcga agggcggcca atacaccgtc tcgtcctatc
     4681 atctgcgggt gccggcgctg ggcgaccgga tgaagcgcgc cgatccggga agccgggtgg
     4741 ccgtcgtctc ggggaaggac cgggcggcga tcatgatggg cggctacggg ccggaccagc
     4801 gctggtggtg ggaccatgac gccaaggcct tcgtcaaccc cggcgcgccg gccgcgccgc
     4861 ggtcggtggc cgaggccaat caggcggtga ccgaggccct gtcgcggccc cgtccggccc
     4921 gcgacctccc cgacgtgtgc gaggcccaca gccgggcggt cccggtgacg ggcggcggca
     4981 agccggtcgg cgcctggcgt ttcgaccgcg cggcggggga caagaacctg ttccgcgcct
     5041 cgcccgattt cgaccgggcc actctcgacc tcggcgaggc gctccggcgc gagatgaagc
     5101 tcggcgaggg cgagtcgacc gacctcctca tcctcggcct gtcggcgacc gactatatcg
     5161 gccactcgtt cggcacccag ggcagcgaga tgtgcatcca gctgctcgcg ctcgacgagg
     5221 ctctgggcgc cttcttccgc aatctcgact ccaccgggat cgactatctg gtgaccctca
     5281 ccgccgatca tggcggagtc gacataccgg agcgcgcgcg cgcgcaggcg tccggcgacg
     5341 cgatgcgcgt cgatccggcc ctcaacgccg gcaacatggc caaggcgctc gcccagcggc
     5401 tcaagctgac cggcgacctg ctctacggcg acggcgcatt cggcgacatg tacgtcgacc
     5461 gcaggctgac ggccgcccag cgcgcgcggg tcctgcgcga ggcggtggcg gcctaccggc
     5521 gccaccccca ggtggcggcg gtgttcacca aggccgagct ggcggcggcg gcggggccgt
     5581 cggggccgcc cgacagctgg agcctcctcc agcgggcgaa ggccagcttc gactccgagc
     5641 gctccgggga cttcgtcgtg ctcctgaagc cccgggtgac tccgatcttc gacaccagtc
     5701 gcggctatgt cgccacccac ggcagcccct gggattacga ccggcgcgtg ccgatcctgt
     5761 tctggcgcaa ggggatggcc cggttcgagc agccgctggc ggtcgagacg gccgacatat
     5821 tgccgaccct ggcggcggtt ctcgccgtgc cgatcgcgcc ggggacgatc gacggccgct
     5881 gcctcgatct cgacgcgggg ccgggcaaca gctgcgtccg gtagcgatcc gggcttcctc
     5941 gcctcccgcg gcaacgcggc ggggcggggc gctgcaagtc agccagccag ggcctcgacc
     6001 ttttccgcca gctcgagcca gcggtgctcc gccgcgtcgc gctccgcccg cgccgcctcg
     6061 atcgccgcgg tgagcgccgc gaagcgccga ggatcgcgcg catagaggtc gggatcgtga
     6121 agggcctcct cgtcgcgggc gatctgcgct tccagagcct ggatccggtc ggggagcagg
     6181 tcgtagtcgc gctggtcctt gtagctcagc ttggtctgcg gccgggcggg cgccgccgcc
     6241 ggcttcgcgg cc
//
LOCUS       DASVQZ010000456         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_864917, whole genome shotgun sequence.
ACCESSION   DASVQZ010000456 DASVQZ010000000
VERSION     DASVQZ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864917"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VF547_12840"
     CDS             complement(<1..968)
                     /locus_tag="VF547_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX8263749.1"
                     /translation="MTSWKVSLPCTRAEAESLKDDISPLAWLDFPPVLMTSESDSARP
                     EQWRLDAYFDREPTTEMIGLLAALAPSAAGTAPAVEKVEERDWVTLSQQGLEPIRAGR
                     FFVHTPRHRGEAPAGAIALEIDAGRAFGTGQHETTSGCLIALSRLKAGGAAVSDLLDL
                     GTGTGLLAFAALRLWPSARAAASDNDPVAIEVSEENAAINGFRLGRARGQLELLVAEG
                     MDHPRLQARSPYDLVVANILAGPLIDLAPSVARAIAPGGRLILAGLLGHQAPAVAGAF
                     RRRGLMLTGRVDRGEWPTLVMRKRRALSSPSAKRRGRGTTRRVVEG"
     gene            complement(997..>1545)
                     /locus_tag="VF547_12845"
     CDS             complement(997..>1545)
                     /locus_tag="VF547_12845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8263750.1"
                     /translation="WSEALDRLGGRIHVLVNNAGIFEAAPVDLPAEEWTAQWERTMRV
                     NLTTSAELCRLAVLHFRASGAGGRIVNVASRAAHRGDSPAHWHYAASKAGMIGMTKSI
                     ARGYAADGVLAFAVSPGFTASEMIDDYMASRGGRQIVADIPLGRVASADEVAETVRWL
                     AVDAPASATGTVIDVNGASYVR"
BASE COUNT          219 a          571 c          539 g          216 t
ORIGIN      
        1 tacccctcca ccacccttcg ggtggtcccc ctccccctcc gcttcgcgga cggggaggac
       61 agggcccgcc gtttgcgcat caccagggtg ggccactcgc cgcgatcgac gcggccggtc
      121 agcatcaacc cccggcgccg aaaggctccc gccaccgccg gcgcctggtg gccgagcagc
      181 ccggcgagga tcagccggcc gcccggagcg atcgccctgg ccaccgacgg cgcgaggtcg
      241 atcagcggcc cggcgaggat gttggcgacg acgaggtcgt agggcgagcg cgcctgcagc
      301 cgcggatggt ccatcccctc ggcgacgaga agctccagct gtccgcgggc gcggccgagc
      361 ctgaagccgt tgatcgcggc attctcctcg ctcacctcga tcgcgaccgg atcgttgtcg
      421 gaagccgcgg cgcgcgccga cggccacagc cgaagcgcgg cgaaggcgag aagcccggtg
      481 ccggtgccga ggtcgagcag atcggacacc gccgctccgc ccgccttcag tcggctgagc
      541 gcgatcaggc agccgctggt ggtctcgtgc tggccggtgc cgaaggcccg gccggcgtcg
      601 atctcaagcg cgatcgcccc cgccggagcc tcgccccggt gcctcggcgt atggacgaag
      661 aagcgcccgg cccggatcgg ctccagcccc tgctggctca gcgtcaccca gtcgcgctcc
      721 tcgaccttct cgacagcggg cgcggtcccg gccgcgctgg gcgccagggc cgcgagcagg
      781 ccgatcatct ccgtcgtcgg ctcgcgatcg aaataggcgt cgagccgcca ttgctcgggc
      841 cgggcggagt ccgactcgct ggtcatcagc accggcggga agtcgagcca ggcgagcggc
      901 gagatgtcgt ccttgagcga ttcggcctcg gcacgagtgc aggggaggct gaccttccag
      961 ctggtcacaa tgctcctccc cgttccgggg aggagctcat ctgacatagc tcgccccgtt
     1021 gacgtcgatc accgttccgg tcgcggaggc aggcgcgtcc acggccagcc agcgcaccgt
     1081 ctcggcgact tcatcggcgc tcgccacccg ccccaggggg atgtcggcga cgatctgccg
     1141 gccgcctcgg ctcgccatat aatcgtcgat catctccgag gcggtgaagc ccggcgagac
     1201 ggcgaaggcg aggacgccgt ccgccgcata gccgcgcgcg atcgatttgg tcatgccgat
     1261 catcccggcc ttggaggcgg cataatgcca gtgcgccggg ctgtcgccgc gatgggcggc
     1321 gcggctggcg acgttgacga tccggccgcc ggctccgctc gcgcggaaat gcagcaccgc
     1381 gagacggcaa agctcggccg aagtggtgag gttgacccgc atcgtccgct cccactgggc
     1441 cgtccactcc tccgccggca ggtcgaccgg cgccgcctcg aagattccgg cattgttgac
     1501 gaggacgtgg atccggccgc cgagccggtc gagcgcttcg ctcca
//
LOCUS       DASVQZ010000457         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_958710, whole genome shotgun sequence.
ACCESSION   DASVQZ010000457 DASVQZ010000000
VERSION     DASVQZ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958710"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF547_12850"
     CDS             complement(<1..331)
                     /locus_tag="VF547_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263751.1"
                     /translation="MLDADAAATEAALPYQGSAAMEALGRFASLGDQPPMRTLCAATI
                     AAGLAGGNRRLARAGLRMLLAHTLATFAKDFVKQRVDRTRPRSKNGEGKDHIPRPGRH
                     TAKETTSF"
     gene            complement(351..1055)
                     /locus_tag="VF547_12855"
     CDS             complement(351..1055)
                     /locus_tag="VF547_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX8263752.1"
                     /translation="MTDDPPFHRCPGAAPRAALFDVDGTLVDTNDLHAAAWREAFLHF
                     GLDRPLEEIRWQVGKGGDNLIPTLFPDLPESRREEIEDFRGDLFKRSFLPRAVPFAGV
                     RPLFERLAADGVLIVLASSSHSEEVDFYLSLIGCEDLVAAKTSKDDVESSKPCPDIFE
                     AALAKAAPFGADEAAVIGDTPWDAKAAARLGLRTIGFRSGGFPDGALTEAGACELYDG
                     PEDLLRRYDTSVFGRG"
     gene            complement(1091..1288)
                     /locus_tag="VF547_12860"
     CDS             complement(1091..1288)
                     /locus_tag="VF547_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263753.1"
                     /translation="MRNAVPSPRPLADPPRLELVLRLADGGPWRLSLSAGMERFIGYY
                     PRVEGLDPGGPLLRFRVADPA"
     gene            1486..2391
                     /locus_tag="VF547_12865"
     CDS             1486..2391
                     /locus_tag="VF547_12865"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8263754.1"
                     /translation="MLAMSLREERQVAEALSPPVAWPTLRLALLLPVAQAALIAAALL
                     GAVPYWAVILPLGYVHFAYYTLVHESIHGNVVRDPRHAWVHQLLGWWGSLNMMTSYPA
                     LRRIHLLHHRHTNTDDDPDYNLCHGPLWKSVGRVVFLRTLLLLPLPIVRLVPAASYTI
                     KQARLTPDEMKIHAVTVTALQLAFWVAVALGYAGIAILLYWIPMMIGLVLLNVFFQWL
                     PHVPFDKTDRYGKARVYLPVAHRILLGQNLHLGHHLWPSVPFYNYRPLNERLRPQFES
                     AGARLDGFLPASGRDQPLRAGLRGS"
     gene            complement(2358..2549)
                     /locus_tag="VF547_12870"
     CDS             complement(2358..2549)
                     /locus_tag="VF547_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263755.1"
                     /translation="MYAAAPRPGSKVQLLLRTPDGGGWGLTLSHGMEQFIGHYPRVAG
                     LGSGGPLLRYEPLSPARSG"
     gene            2745..>3471
                     /locus_tag="VF547_12875"
     CDS             2745..>3471
                     /locus_tag="VF547_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8263756.1"
                     /translation="MRRSLVETLFASATSLGAGAVAGAACSTAIALASGDPWLTTLAV
                     AIALTGALRLAHAVFFHHVDDQDPSKSARSETVYELGAWTYAALLGAEVMITLMRTDE
                     ALYHLLAAVLTTGYAAGICGRNAGRPVIAIGQLALASTPMSIGLFLSSDPLRWVLGAV
                     NCAFIVGMVDVTLQTYSAVLKAMSATREQKSLTDRFERLARFDAMTGLENRGAFQERL
                     EMELAAVAADGGQLAVLWVDLDRF"
BASE COUNT          514 a         1216 c         1199 g          542 t
ORIGIN      
        1 gaaagctggt cgtctccttg gcggtatggc ggcccggcct cgggatatgg tccttcccct
       61 cgccgttctt cgaacgcggc cgggtgcggt cgaccctctg cttgacgaaa tccttggcga
      121 acgtcgccag ggtgtgggcg agcagcatcc gcaggccggc gcgggcgagc cggcggttgc
      181 cgccggcgag gccggccgcg atcgtcgcgg cgcacagggt gcgcatcggc ggctggtcgc
      241 cgagcgaggc gaaacggccg agcgcctcca tcgccgccga gccctggtag ggcagcgccg
      301 cctcggtcgc cgcggcgtcg gcgtcgagca ccgcccggcc tgccttcgtc ctagccacgg
      361 ccgaagaccg acgtgtcgta gcggcgcagc aggtcctcgg gcccgtcgta gagctcgcag
      421 gcgccggcct cggtcagcgc gccgtcgggg aagccgccgc ttcgaaagcc gatcgtccgc
      481 aggccgagcc gagccgccgc cttcgcgtcc cagggcgtgt cgccgatcac cgccgcctcg
      541 tccgcgccga acggggccgc cttggcgagc gccgcctcga agatgtcggg gcagggcttg
      601 gagctttcga cgtcgtcctt cgaggtcttg gcggcgacga gatcctcgca gccgatcagg
      661 ctcaggtaga aatccacttc ctccgaatgg gacgaggacg cgagcacgat cagcacgccg
      721 tcggcggcca gccgctcgaa cagcggccgg actccggcga acgggacggc gcggggcagg
      781 aagcttcgct tgaacaggtc gccgcggaaa tcctcgatct cctcgcgcct cgattccggc
      841 aggtcgggga acagggtcgg gatgagattg tcgccgccct tgccgacctg ccagcgaatc
      901 tcctcgagcg gccggtccag cccgaaatgg aggaaggcct cgcgccaggc cgcggcatgg
      961 aggtcattgg tgtcgacgag cgttccgtcg acatcgaaaa gcgccgcccg cggggcggcg
     1021 ccggggcatc ggtggaaggg tggatcgtcc gtcatcgccc tcccaacgcc gggaggcgcg
     1081 gcaatttccc tcaggccggg tcggcgaccc ggaagcggag cagcggcccg ccggggtcga
     1141 ggccctcgac gcgcggataa tagccgatga agcgctccat tccggccgac aggctcagcc
     1201 gccacgggcc gccgtcggcg aggcgcagca ccagttccag gcggggcggg tccgccaggg
     1261 gacggggcga gggaacggcg ttgcgcaccc cgccaccatg acagcgacag gttcacaaag
     1321 ggtttccgaa agctcgtaac tcccgccgtc ggccctgtgc cgttccgtga cggcggcggc
     1381 gacggcgacc ggacccgaag cggagagctt ccttcggcct ccaactgtgg taaaacccgc
     1441 cgaaaccata ttgggggccg cgagggcgcg gcgggggagc atcgaatgct ggcaatgtcg
     1501 ctgagggagg aacggcaggt ggcggaggcg ctgtccccgc cggtggcatg gccgaccctg
     1561 cgcctcgcac tgcttctgcc cgtcgcgcag gcggcgctga tcgccgccgc cctgctcgga
     1621 gcggtgccct attgggcggt gatcctgccg ctcggctacg tgcatttcgc ctattacacg
     1681 ctggtccacg agagcatcca cggcaatgtc gtgcgcgacc cccgccacgc ctgggttcat
     1741 cagctgctcg gctggtgggg ctcgctcaac atgatgacca gctatccggc gcttcgccgg
     1801 atccacctcc tccatcaccg ccacaccaac accgacgacg atccggacta caacctgtgc
     1861 cacggaccgc tctggaagag cgtcggccgg gtcgtcttcc tgcgcaccct gctcctgctt
     1921 ccgctgccga tcgttcggct ggtgccggcg gcgagctaca cgatcaagca ggcgcggctc
     1981 acgcccgacg agatgaagat ccatgccgtc accgtgaccg cgctgcagct cgccttctgg
     2041 gtcgccgtgg cgctcggcta tgccgggatc gccatattgc tctactggat accgatgatg
     2101 atcggcctgg tcttgctgaa cgtcttcttc cagtggctgc cgcacgttcc gttcgacaag
     2161 accgaccgct acggcaaggc gcgggtctac ctgcccgtcg ctcatcgcat cctgctcggg
     2221 cagaacctgc atctcggcca ccatctgtgg ccgtcggtgc cgttctacaa ttaccgcccg
     2281 ctcaacgagc ggcttcgtcc gcagttcgaa agcgcgggcg cccggctcga cggcttcctc
     2341 ccggcctcgg ggcgggatca gccgctgcgg gcggggctga gaggctcgta gcggagcagc
     2401 ggaccgccgg atcccagccc ggcgacgcgc ggatagtgcc cgatgaactg ctccatcccg
     2461 tgcgacaggg tcaggcccca gccgccgcca tccggcgtgc gcagaaggag ctggactttc
     2521 gagccgggcc ggggggccgc ggcgtacatc ggtgaggcca tgcgctgaaa accgccgccg
     2581 ccacgggcgg gttcctcggc ccgtcgcgag ggcgggatcg atcgccctta attcaagata
     2641 aagaagtttc cgccacaaga accgctcctc gggggagaca cgtggcaagc atcgtcgcga
     2701 agatgcgcgg tatcgctaaa accgatcgga cggtcgcgga ggaagtgcgc cgttcgctgg
     2761 tcgagacgtt gttcgcctcc gccacgtcgc tgggggcggg cgccgtcgcc ggcgccgcct
     2821 gcagcacggc catcgcgctt gcgagcggcg acccctggct gaccaccctc gccgtcgcga
     2881 tcgcgctgac cggcgccctg cgcctcgccc acgcggtctt cttccaccat gtggacgatc
     2941 aggatccttc caagagcgcc cggtcggaga cggtctacga gcttggggcc tggacctatg
     3001 ccgcgctgct cggcgccgag gtcatgatca ccctgatgcg gacggacgag gcgctctacc
     3061 acctcctcgc ggcggtcctg acgacgggct atgccgccgg catctgcggg cgcaacgccg
     3121 gccggccggt gatcgccatc ggccagctcg ccttggcgtc gacgccgatg tcgatcggcc
     3181 ttttcctgag ctcggacccg ctccgatggg tgctgggggc ggtcaattgc gccttcatcg
     3241 tcggcatggt cgacgtcacc ctccagacct attcggcggt gctcaaggcg atgtcggcga
     3301 cccgcgagca aaagagcctg accgaccgtt tcgagcggct ggcgcgcttc gatgcgatga
     3361 ccgggctcga gaatcgcggc gccttccagg aacggcttga gatggagctt gccgcggtgg
     3421 ccgccgacgg cggccagctc gccgtcctgt gggtcgatct cgaccgcttc a
//
LOCUS       DASVQZ010000458         5483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_959232, whole genome shotgun sequence.
ACCESSION   DASVQZ010000458 DASVQZ010000000
VERSION     DASVQZ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959232"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VF547_12880"
     CDS             complement(<1..1484)
                     /locus_tag="VF547_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEX8263757.1"
                     /translation="MATSPSENAYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHM
                     VFEVSDNAIDEALAGHCDLIRITLNPDGSVSVEDNGRGIPTGIHAEEGVSAAEVIMTQ
                     LHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLDLTIWRDGEEHYMRFRHGDAE
                     APLKVVGPAGEKKGTRVTFLPSPETFKITEFDWDTLEHRFRELAFLNSGVRLILTDAR
                     HEDEKSVELYYEGGISAFVRYLDRTKTALIPDPISVTGQRDDVGIEVALEWNDSYYEQ
                     TLCFTNNIPQRDGGTHLAAFRAALTRTLNNYADKSGMMKREKVSLTGDDMREGLTAIV
                     SVKLPDPKFGSQTKDKLVSSEVRQPLESLMADKLAEWLEENPGHARTIIQKIIDAAAA
                     REAARKAREASRKSVLGVASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRNRHN
                     QAILPLKGKILNVERARMDRMLSSKEVGTIIQALGTGIATGMDRS"
     gene            1826..2047
                     /locus_tag="VF547_12885"
     CDS             1826..2047
                     /locus_tag="VF547_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263758.1"
                     /translation="MTLRSALAAALALAAPAAAHVPVAQMAPAIYTDPPHDKAHPAAM
                     EVLHIPSGGSGSTASPTKRPAPVRTRCWC"
     gene            2094..2696
                     /locus_tag="VF547_12890"
     CDS             2094..2696
                     /locus_tag="VF547_12890"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8263759.1"
                     /translation="MRRAGWTVVTFNYRGSWGSPGRFRFAGNLEDARAVLAYLRNPAN
                     SARLAVDTKRIVVAGHSMGGWVAAMTAERDPALAGAILISAANMGGRKMTRKEAVALM
                     ADNMEALAGVTAESMADEMLASTAAFDWTPGAAGLARHPLLVLTSDDGLGPMSDALAA
                     AVKAAPGARVSAVHAGTDHGWSSARIRLAHEVITWLQALP"
     gene            complement(2884..3939)
                     /gene="recF"
                     /locus_tag="VF547_12895"
     CDS             complement(2884..3939)
                     /gene="recF"
                     /locus_tag="VF547_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX8263760.1"
                     /translation="MALARVALTDFRNHRDALLAPGPGLVVLTGENGAGKTNILEAVS
                     LLSPGRGLRGAALSEMARTGGSGGFAVAARLGETDIGTGTSPSAPERRQVRINGAPAS
                     ATSLSEWLSVLWLTPAMDRLFQEGAGGRRRFLDRLVLALEPGHAVHAARYEAAMRARN
                     KLLAEPGPPDEAWLSALEARMAEHGDALREARARTVALLAGRLAGAPEGPFARAGLAL
                     EGGDEDLERDLARGRARDAAAGRTLAGPHRTDLAVTHAGKGQPAVHCSTGEQKALLLA
                     IVLAHADLVAERSGRRPVLLLDEVAAHLDPRRRAALFERLEAGGGQVWMTGTEPAMFD
                     GLGSAASRFELSDGAIA"
     gene            complement(4120..4869)
                     /locus_tag="VF547_12900"
     CDS             complement(4120..4869)
                     /locus_tag="VF547_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEX8263761.1"
                     /translation="MASQSFNPAVPMSRDWGTAFRALRRLLANGDDTVQVFRIMRALN
                     GDTSHRNYRRLIATMEGGRLAYERVELAPRLCDRAWIDSFPEGSVGAAYRAFLDRTGY
                     SADGLAEVSVAETDIPPNVEHPYAWFGRRERDIHDIWHVLTGYEADEPLGEACLVAFS
                     YAQTGGLGWAFIGAGAALKSLRITRGTAFARAVWEGWRHGKRAAWLHAEDYEALMGEP
                     LDSARARLRIAEPRRYREAQRSLAAAGLTGI"
     gene            complement(4927..5361)
                     /locus_tag="VF547_12905"
     CDS             complement(4927..5361)
                     /locus_tag="VF547_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263762.1"
                     /translation="MAEEARGTGIYAAALVVTIVCLAVANFVIAWLGGLAHVEAARPA
                     AAALAFAQGLVGALLAGFASRQLFKSVGPADFWLKWIVLLVAALFAIAIVWLLVTGRS
                     GRALNWTTYAGLAALLGTEVGRRLARGLFRRPASDPVAASPS"
     gene            complement(5364..>5483)
                     /locus_tag="VF547_12910"
     CDS             complement(5364..>5483)
                     /locus_tag="VF547_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX8263763.1"
                     /translation="LGQIEGDHVEMHLADPAAPTLLRENDKAAALYVLMPMRV"
BASE COUNT          825 a         1847 c         1981 g          830 t
ORIGIN      
        1 gaacggtcca tgccagtagc gatgccggtg ccgagcgcct ggatgatggt gccgacttcc
       61 tttgaagaca gcatgcggtc catgcgggcg cgctcgacgt tcaggatctt gcccttcagc
      121 ggaaggatgg cctgattgtg ccgattgcgg ccctgcttgg cggagccgcc ggcgctgtcg
      181 ccctcgacca ggaagagctc gcacttggcg gggtcgcgct cctggcagtc ggcgagcttg
      241 cccgggaggg aagcaactcc gagcaccgac ttgcggctcg cctcgcgcgc cttccgggcc
      301 gcctcgcggg cggcggcggc gtcgatgatc ttctggatga tcgtcctcgc gtggccggga
      361 ttctcctcga gccattcggc gagcttgtcg gccatcaggc tctcgagcgg ctggcgcacc
      421 tcggagctga cgagcttgtc cttggtctgc gagccgaact tggggtcggg cagcttgacc
      481 gagacgatcg cggtcagccc ttcgcgcatg tcgtcgccgg tgaggctgac cttctcgcgc
      541 ttcatcattc ccgacttgtc ggcataattg ttcagcgtgc gggtcaaagc ggcgcggaag
      601 gcggcgaggt gggtgccgcc gtcgcgctgg gggatgttgt tggtgaagca gagggtctgc
      661 tcgtaatagc tgtcgttcca ctcgagcgcg acctcgatgc cgacgtcgtc gcgctggccg
      721 gttaccgaga tcgggtccgg gatcagcgcc gtcttggttc ggtcgagata gcggacgaag
      781 gccgagatcc cgccctcgta atagagctcg accgatttct catcctcgtg ccgggcgtcg
      841 gtgaggatca gccggactcc ggagttgagg aaggcgagct cgcggaagcg gtgctcgagc
      901 gtgtcccagt cgaactcggt gatcttgaac gtctcgggcg agggcaggaa ggtgacccgg
      961 gtgcccttct tctcgccggc cgggccgacg accttcaacg gcgcctcggc gtcgccgtgg
     1021 cggaagcgca tgtaatgctc ctcgccgtcg cgccagatgg tgaggtcgag ccattcggac
     1081 agcgcgttga ccacggagac cccgacgccg tgcaggccgc cggagacctt gtaggcattg
     1141 tcgtcggacg tgttctcgaa cttacccccg gcgtggagct gggtcatgat cacctcggcc
     1201 gccgacacgc cctcctcggc gtggatcccg gtcgggatgc cgcggccgtt gtcttcgacg
     1261 gagaccgacc cgtcggggtt caaggtgatg cggatgaggt cgcaatgacc ggccagggcc
     1321 tcgtcgatcg cgttgtcgga gacctcgaag accatgtggt ggaggcccga gccgtcgtcg
     1381 gtgtcgccga tatacattcc gggccgcttg cggacggcgt cgaggccctt cagcaccttg
     1441 atggagtcgg cgccgtatgc gttttcgctg ggggatgttg ccatcatcgg gatatagcca
     1501 ttggagggcc gaaacccaag gaaattggcc ggattccggc cccttttttc gcagttgcag
     1561 catcggccgg gagcgacggc cggttatgca agctgagccg ccgcctttat acgcccgccg
     1621 cataattatg cggaaaatgt ggattgtcgc cgctggaccc cgccggctcg gatcccctgt
     1681 ctccgttcgg gctgagcttg tcgaagccct ctcctttcgc cttgcaaaac gggaaggaag
     1741 gcccttcgag aggctcagga cgaacgggtt cgagtgattc aacctgttcg cacatcgctc
     1801 cacgccgaac gaagggagac aagacttgac gttgagatca gccctcgcag cggccctggc
     1861 gctcgccgcg ccggcggccg cgcacgttcc ggtcgcgcag atggcccccg ccatctacac
     1921 cgatcccccg cacgacaagg cccatccggc ggccatggag gtgctccaca tcccctcggg
     1981 cgggtcagga tcaacggcct cgcctacaaa gcggccggcg ccggtccgca cccggtgctg
     2041 gtgctgatgc acggcctgcc ggggaacgag aagaacctcg atctcgccca gcggtgcggc
     2101 gggcgggctg gaccgtggtc acgttcaact atcgcggctc ctggggcagc ccggggcggt
     2161 tccgcttcgc cggcaatctc gaggatgccc gggccgtcct cgcctatctt cgcaatcccg
     2221 ccaattcggc gaggctggcg gtggatacga agcggatcgt cgtcgccggc cattcgatgg
     2281 gcggctgggt cgcggcgatg accgcggagc gcgatccggc cttggccgga gcgatcctca
     2341 tctcggcagc taatatgggc gggcgcaaga tgacgcgcaa agaggcggtc gcgctgatgg
     2401 ccgacaatat ggaggcgctg gccggcgtga ccgcggagag catggccgac gaaatgctgg
     2461 ccagtaccgc cgccttcgac tggacccccg gcgcggccgg gctcgcccgg catccgctcc
     2521 tcgtcctcac ctccgacgac gggctcgggc cgatgagcga cgcgctcgcg gcggcggtga
     2581 aggccgcgcc gggggcccgg gtctcggcag tccatgccgg caccgaccac ggctggtcga
     2641 gcgcccgcat ccgcctcgcc cacgaggtga tcacctggct ccaggcactg ccctaggaca
     2701 tcgaggggct ggatcgaatc tcccgcatcc gtccgccctg agcttgtcga agggcctgcc
     2761 tgttcgatgc gcggaggagg agaggccttc gacgagctca gccggaacgg agacggggtg
     2821 ccggttcgac aagctcgcgg ctagtcggga tgagcggatc gggatcgacc tgacgatcat
     2881 tcgctaggcg atcgcgccgt cggacagctc gaagcggctc gcggcggagc ccaggccgtc
     2941 gaacatggcc ggttccgtgc ccgtcatcca gacctggccc ccgcccgcct cgagccgctc
     3001 gaacagcgcg gcccgccgcc gcgggtcgag atgggcggcg acctcgtcga ggaggaggac
     3061 cgggcggcgg ccggagcgct cggcgacgag gtcggcatgg gcgaggacga tcgcgagcag
     3121 aagcgccttc tgctcgccgg tcgagcaatg cacggcgggc tggcccttgc cggcatgggt
     3181 gaccgcgagg tcggtgcggt gcgggccggc cagggtccgg ccggcggcgg cgtcgcgggc
     3241 gcggccgcgc gcaaggtcgc gctccagatc ctcgtcgccg ccttccagcg cgaggccggc
     3301 gcgggcgaac gggccttcgg gcgcgccggc gagacgcccg gcgagcagcg cgacggtgcg
     3361 cgcccgcgcc tccctcaagg catcgccgtg ctcggccatc cgcgcttcca gcgccgacag
     3421 ccaggcctcg tcgggcggac cgggctcggc cagcagcttg ttcctcgccc gcatcgcggc
     3481 ctcgtagcgg gcggcgtgga cggcgtggcc gggctccagc gccagcacca gccggtcgag
     3541 gaagcggcgg cggccgccgg cgccttcctg gaacaggcgg tccatcgccg gggtgagcca
     3601 gagcacggag agccactcgg aaagcgaggt ggccgaggcc ggagcgccgt tgatccggac
     3661 ctggcggcgc tccggcgccg acggcgaggt gccggtgccg atgtcggtct cgccgagccg
     3721 cgccgcgacc gcgaagccgc cgcttccccc ggtcctcgcc atctcggaca gggccgcgcc
     3781 gcgcagaccg cgcccgggcg agagcagcga caccgcctcg agtatgttgg tcttgcccgc
     3841 gccgttctcg ccggtgagca cgaccaggcc cgggccgggg gcgagcagcg cgtcgcggtg
     3901 gttgcggaaa tcggtcaggg cgacgcgggc gagggccatc gcccgtccct agcggaagcg
     3961 gcgggccgcg aacaatgcgg gccaaattcc tccccggcac tggccgggga gggggaccag
     4021 gcgaagcctg gtggaggggt ctctcggaaa ccgaagaacc cctccaccgc gcttcgcgcg
     4081 gtccccctcc cccggaaatc cgggggagga actcagagtt cagatcccgg tcagcccggc
     4141 ggcggcgagg ctgcgttgcg cctcgcggta gcgcctcggc tcggcgattc gaagcctcgc
     4201 ccgcgccgaa tccagcggct cgcccatcag cgcctcgtaa tcctcggcgt ggagccaggc
     4261 ggcgcgcttg ccgtggcgcc agccttccca gaccgctcgg gcgaaggcgg tgccgcgggt
     4321 gatccgaagg ctcttcagcg ccgcgccggc gccgatgaag gcccagccga gcccgccggt
     4381 ctgggcgtag gagaaggcga ccaggcaggc ctcgccgagc ggctcgtcgg cctcgtagcc
     4441 ggtcagcaca tgccagatgt catggatgtc gcgctcgcgc cggccgaacc aggcataggg
     4501 atgctcgaca ttgggaggaa tgtcggtctc ggcgacgctg acctcggcga ggccgtctgc
     4561 cgaatagccg gtgcggtcga ggaaggcgcg gtaggcggcg ccgacgctgc cttccgggaa
     4621 ggagtcgatc caggcgcggt cgcagagccg cggcgccagc tccacccgct cataggcgag
     4681 gcggccgcct tccatggtgg cgatcaggcg ccggtagttg cggtgggacg tgtcgccgtt
     4741 cagcgcccgc atgatcctga agacctgaac ggtgtcgtcg ccattggcga gcagccggcg
     4801 caacgccctg aaggccgtgc cccagtcgcg gctcatcgga acggcgggat tgaacgactg
     4861 gctcgccatc ggcgactcca ctgctgacac ggatgtcagt gtggcacgcc cggcggcccg
     4921 tttcaatcag gaaggagagg cggcgaccgg gtcggaggcg ggccggcgga agaggcccct
     4981 ggcaagcctt cggcccacct cggtgccgag gagcgccgcg aggccggcat aggtcgtcca
     5041 gttcaacgcc cgaccggacc tgccggtgac cagcagccag acgatcgcga tcgcgaacag
     5101 ggccgcgacc agcaggacga tccatttgag ccagaaatcg gcgggaccga ccgacttgaa
     5161 cagctgcctc gacgcgaagc cggccaacag ggctccgacc aggccctggg cgaaggccag
     5221 agcggcggcg gcgggccttg cggcctcgac gtgcgccaga ccgccgagcc aggcgatgac
     5281 gaaattggcg acggcgaggc agacgatcgt gacgaccagg gccgccgcat agatcccggt
     5341 gccgcgggct tcctcagcca tggtcacacc cgcatcggca tcagcacgta gagcgccgcc
     5401 gccttgtcgt tctcgcgaag caaagtcggt gccgccgggt cggcgaggtg catctcgaca
     5461 tgatcgccct cgatctgccc gag
//
LOCUS       DASVQZ010000459         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_575665, whole genome shotgun sequence.
ACCESSION   DASVQZ010000459 DASVQZ010000000
VERSION     DASVQZ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575665"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VF547_12915"
     CDS             complement(<1..229)
                     /locus_tag="VF547_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263764.1"
                     /translation="MAAVGAGALLTGAMANAAETLTDALVKTYAGNPTIMAQRAQLRS
                     LDESVAIARAQGRPQLSGNVGLNQDVISSNTG"
     gene            complement(262..927)
                     /locus_tag="VF547_12920"
     CDS             complement(262..927)
                     /locus_tag="VF547_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX8263765.1"
                     /translation="MRQREMTEHNFEHMRRAMIASQLRTTGTNDPRVLAAMGEVPRER
                     FVPAERISLAYADALVPLKPGRDFNSPMALGRLLTEAAPRPGERALAVGTATGYAAAV
                     LARLVGRCVAVEEDAELAEAARANLAGYAVELVEGPLAEGCAARGPYDLILIDGAVEL
                     VPEALVAQLADGGRLVAGLLENGVTRLAIGRRAGDGFGMAAFADAAAAILPGFAKPRA
                     FKF"
     gene            1179..1252
                     /locus_tag="VF547_12925"
     tRNA            1179..1252
                     /locus_tag="VF547_12925"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1211..1213,aa:Cys,seq:gca)
     gene            1379..>1852
                     /locus_tag="VF547_12930"
     CDS             1379..>1852
                     /locus_tag="VF547_12930"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263766.1"
                     /translation="MSFQSTMIRAGALAAASTFAIALASPASAQGATCATYAAGNPVA
                     PAQGTATAVGQTACGNLAAATGVSATAVGDRAVASGNFASAFGDFAAATGIQSTAVGD
                     VASALSTNATAVGRNANVVAAATNGTALGSETLSGGVNSTAVGWRAGATGASSTAV"
BASE COUNT          266 a          697 c          622 g          267 t
ORIGIN      
        1 cgccggtatt gctgctgatc acgtcctggt tgaggccgac gttgccggag agctggggac
       61 ggccctgcgc ccgggcgatg gcgacgctct cgtcgagcga gcgcagctgg gcgcgctggg
      121 ccatgatcgt gggattgccg gcataggtct tcacgagcgc gtcggtgaga gtctcggcgg
      181 cgttcgccat cgctcccgtc agcagcgccc ccgcccccac ggcggccatc aaccctcccc
      241 gcattttcct cgactccact gtcaaaattt gaacgcccgc ggtttcgcga agccgggcag
      301 gatcgccgcc gccgcgtcgg cgaatgccgc cattccgaac ccatcgcccg cccggcggcc
      361 gatggcaagc cgggtgactc cgttttccag aagccccgcg acgagccgtc cgccgtcggc
      421 gagctgggcc accagcgcct ccgggacgag ctcgaccgcg ccgtcgatca ggatgaggtc
      481 gtaggggccc cgggccgcgc agccctcggc gagcggtcct tcgaccagct ccaccgcgta
      541 gccggcgagg ttggcgcggg cggcctcggc cagctccgca tcctcctcga ccgcgacgca
      601 ccggccgacc agccgcgcaa ggacggcggc ggcgtagccg gtggcggtcc cgaccgccag
      661 tgcgcgctcg ccgggccgcg gcgccgcctc ggtcagcagc cgccccagag ccatcgggct
      721 gttgaagtcg cggccgggct tcaggggcac cagcgcgtcg gcataagcga gcgagatccg
      781 ctcggccggc acgaaccgct cgcgcggcac ctcgcccatg gcggcgagga cccgcggatc
      841 gttggtgccg gtggtccgca gctggctggc gatcatcgcg cggcgcatat gctcgaaatt
      901 atgctcggtc atctcacgct gcctcatcgc acaactgtct tactgacgca atacaccttg
      961 tcgccttggc ggcttttatc ggcaagccgg cggcaggcca agcggagagt ccggtcatga
     1021 gccttggggc agcaagaccc gtgccagagc cgccgggtcc gcgccgcggg cgccgccgcc
     1081 gccgccgccc tgtccgattg cggacaccgg cgtccgcggg cggacaggag cgaagacggt
     1141 tgacttggcg ctccatcatg cacaagtccg gccgcggcgg cccgatggcg gagtggttac
     1201 gcagaggact gcaaatcctt gcacgccggt tcgattccgg ctcgggcctc catcttccgc
     1261 taaattcggc gacagcttcc gcgcaaagga aggatttgcg ttgccaattc ccttgcccgg
     1321 caactaaaag gccgcagacg cgcctcacaa gagccgactc agcagggagt gaaccataat
     1381 gtcctttcaa agcacgatga tccgggccgg agccttggct gccgccagca ccttcgccat
     1441 cgccctcgcc tcgccggcgt cggcccaggg tgcgacctgc gccacctatg ctgccggcaa
     1501 ccccgtcgct ccggcgcagg gcacggcgac cgccgtcggc cagaccgcct gcggcaatct
     1561 cgcggccgcg accggagtct ccgcgaccgc cgtcggcgac cgcgccgtcg cctcgggcaa
     1621 cttcgcgtcg gccttcggtg acttcgccgc cgcgaccggg atccagtcga ccgcggtcgg
     1681 cgacgtcgcc agcgccctgt cgacgaacgc cacggcggtc ggccgcaacg ccaacgtcgt
     1741 cgccgccgcc accaacggca cggcgctggg cagcgagacc ctctcgggcg gcgtgaattc
     1801 cacggccgtg ggctggcgcg ccggcgccac cggcgcctcg tctaccgccg tc
//
LOCUS       DASVQZ010000460         5400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allosphingosinicella sp. isolate SMAG_U3221
            k141_576259, whole genome shotgun sequence.
ACCESSION   DASVQZ010000460 DASVQZ010000000
VERSION     DASVQZ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959846
            Sequence Read Archive: SRR10953934
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allosphingosinicella sp. (soil metagenome)
  ORGANISM  Allosphingosinicella sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Allosphingosinicella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:44:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,592
            CDSs (total)                      :: 2,556
            Genes (coding)                    :: 2,536
            CDSs (with protein)               :: 2,536
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 3 of 20
            Pseudo Genes (incomplete)         :: 16 of 20
            Pseudo Genes (internal stop)      :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Allosphingosinicella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576259"
                     /isolate="SMAG_U3221"
                     /isolation_source="soil"
                     /db_xref="taxon:2823234"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="34.7856 N 115.66 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VF547_12935"
     CDS             <1..937
                     /locus_tag="VF547_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HEX8263767.1"
                     /translation="EAASAAAAAAAAERQGEEALRETEARLAQVGEALATAREARAGA
                     AARADNQDSRRVEMGRISGERFECPPPLLPERLGFDEATIVPAAEESGRHERLSAERE
                     RIGPVNLIAERELAELDESRAASQAEREELGLAINRLRGSIGSLNREGRARLLTAFET
                     VDRHFRSLFTTLFEGGQAHLELVESDDPLEAGLEIMAQPPGKRLATLTLLSGGEQALT
                     AIALIFALFLTNPAPICVLDEVDAPLDDANIERFCDLLDRMVNETDTRYLIVTHNAVT
                     MSRMHRLFGVTMIEKGVSRLVSVDLGGAERLLAAE"
     gene            943..1497
                     /locus_tag="VF547_12940"
     CDS             943..1497
                     /locus_tag="VF547_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263768.1"
                     /translation="MAAGSRAWPALLPLVLTACWVGEPFYAAEEVRAPIAPGLYRTAG
                     TESPSDRSRYRVSVRRDGYTSLAQLDAGETTTVGFAPLPGEAGVFVAWFQESVEKGWE
                     PGTLPYGLLERRGREYRLSFPMCRETRALAEAAGAVFLPDPKTPLCRFADRKSLEAGL
                     RRAAAEGPVESLRLVLAGEGEEDD"
     gene            1622..2053
                     /locus_tag="VF547_12945"
                     /pseudo
     CDS             1622..2053
                     /locus_tag="VF547_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
     gene            2292..2825
                     /locus_tag="VF547_12950"
     CDS             2292..2825
                     /locus_tag="VF547_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8263769.1"
                     /translation="MSWALFLLAEQPELQERLAGEAASALAEGEGADLPARLPKLRLA
                     LEETLRLYPSAPRFDRQAVESDRLCGEEVRPGDIVSIWPWLLHRHKALWDDPDVFDAE
                     RFAEGSRHRFQYIPFGGGPRLCVGARFATAEALTILAHWLSHWRFRPVPGHVVRVHGM
                     VTLRPAGGLPLILSRRD"
     gene            2977..5103
                     /locus_tag="VF547_12955"
     CDS             2977..5103
                     /locus_tag="VF547_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX8263770.1"
                     /translation="MYRRTVSMAALTAALAMPASAAELAATAAAAADAEPTIVVTGQR
                     PEYGAQETCTATRTCTEVKDVPQSLSVVTESQIEDQAMRSIADVLMYVPGATPGTGEG
                     NRDQITLRGNNTTADFFVNGVRDDVQYFRDLYNAERIEVLRGPNAMIFGRGGGGGVVN
                     RVTKRSSFGTYRELAVQGDSEGGLRLTGDVDQPLGESIGLRLNGVYENGESFRRHVEL
                     ERFGINPVLGARLGDTRIDLSYEYFHDRRTADRGLPAAGSRPLRGADRTFFGDPDLSF
                     ADVDAHVASLAVEHRFSDALTLRNRTSYGDFDKFYQNIFPSALNTATGLVSVSAYNDS
                     VDRQNLFSQTDLIVEGDLGGFSHTLLLGFEIGRQETLSRRRNGFFTSAANPLGVPSIS
                     VPVTSPTIDASLVFLPVNTNPARTPANFNESEAKVLAFYAQEQLRLSDAIEIVAGLRY
                     DRFELDVLNRNTGQTFSRTDELLSPRLGLVVKPMPSLSLYASYGRSYLPSGGDQFTSL
                     DLTGAALKPERFDNYEIGAKWEPVQGLLATVALYQLDRTNTRAAGPTPGTVVLTGAQR
                     SRGLELGLERNVTDRWQVSAGYALQEARIRRTTAAAPAGREVPLVPKHQFSVWNRYNV
                     DRGLGFGLGVVAASRSYASISNAVTLPGYARVDAAVFYELTDGIEAQVNVENLLGADY
                     FPTAHNDNNIAPGAPTTARATLRFNF"
     gene            5194..>5400
                     /locus_tag="VF547_12960"
     CDS             5194..>5400
                     /locus_tag="VF547_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8263771.1"
                     /translation="MIKSLLTMVALLSAAPASAQAGTQAGGSIRSNDQIRGALFGSTM
                     SAQAEMRGAAHRRESQRRTVSDGTA"
BASE COUNT          809 a         1978 c         1812 g          801 t
ORIGIN      
        1 cgaggccgct tcggccgccg cggcggcggc ggcggcggaa cgccagggcg aggaagcctt
       61 gcgcgagacc gaggcccggc tggcgcaggt cggcgaagcg ctcgccaccg cccgcgaggc
      121 acgcgccggc gctgccgccc gcgccgacaa tcaggacagc cgccgcgtcg agatgggccg
      181 catttccggc gagcggttcg aatgcccgcc gccgctcctg cccgagcggc tcggcttcga
      241 cgaagccacc atcgtccccg cggccgagga gtcgggccgc catgagcggc tctcggccga
      301 acgcgagcgg atcggcccgg tcaacctcat cgccgagcgc gagctggccg agctcgacga
      361 gagccgcgcc gcctcgcagg ccgagcgcga ggagctcggc ctcgcgatca accggctgcg
      421 cggctcgatc ggcagcctca accgcgaagg gcgcgcccgc ctgctcaccg ccttcgagac
      481 ggtggatcgc catttccgaa gcctgttcac gacgctcttc gaaggcggcc aggcccatct
      541 ggagctggtc gagtcggacg atccgctcga ggccgggctc gagatcatgg cccagccgcc
      601 cggcaagcgc ctcgccaccc tcaccttgct ctccggcggc gagcaggcgc tgaccgccat
      661 cgccctcatc ttcgcccttt tcctcaccaa tcccgccccg atctgcgttc tcgacgaagt
      721 cgacgccccc ctcgatgacg ccaacatcga gcgcttctgc gacctgctcg accggatggt
      781 gaacgagacc gacacccgct acctgatcgt cacccacaat gcggtgacga tgagccgaat
      841 gcaccgcctg ttcggagtca ccatgatcga gaagggcgtc agccgcctgg tcagcgtcga
      901 tctcggcggc gccgagaggc tgctggcggc ggagtaggga aggtggcggc cgggtcccgc
      961 gcctggccgg cgcttctgcc gctggtcctg accgcctgct gggtcgggga gcccttctac
     1021 gccgcggaag aggtccgggc gccgatcgcg ccgggcctgt accggacggc cggcaccgaa
     1081 tctccctccg accggagccg ctaccgggtg tcggtccgcc gcgacggcta tacgtcgctg
     1141 gcgcagctcg acgcgggcga gacgacgacg gtcggcttcg cgccgctccc cggcgaagcc
     1201 ggcgttttcg tcgcctggtt ccaggaatcg gtcgagaagg gctgggagcc gggaaccctg
     1261 ccttacgggc tgctcgagcg gcgcggtcgg gaatatcggc tcagcttccc gatgtgccgc
     1321 gagacccggg cgctggcgga agcggcgggg gcggtgttcc tgccggatcc caagaccccc
     1381 ctgtgccgct tcgccgaccg caagagcctg gaggccgggc ttcgcagggc cgcggccgag
     1441 ggtccggtcg aatcgcttcg cctcgtcctg gccggcgagg gtgaagagga tgactaagcc
     1501 cttcatcccg cctcatccgc cacgcagccc ggggcccgtc gccagctggc gcggcttctt
     1561 cggcgagcgg gcccggaccg ccgtctacgg ctggtcggag caggccttcc ggctgcccta
     1621 catgcggcgc aagatcctcg gcttccgggt ccacatcccg ctcgagccgg acgacgtgca
     1681 gcatgtgctg ctcgacaatg cggccaatta cgccaagccc gacgtcgtca aggcgctgct
     1741 cgcccccgcc atcgggcagg ggctgctgac ctcggacggc agcctgtggc gcgagcagag
     1801 gcggatcgtc gccgccaatt tcgcgccggc cgccgtcgac gccctggtcc cggagttcgg
     1861 cgcggtcggc cgggaggcgc tcgggcgctg gcagccgggc gctcggaaca tggccgtcga
     1921 ggcgacccag gcgacgatgc gggtcatctc caacaccttg ttcgggggcg acgcccggct
     1981 caccagcgac gccgccatgg cgcatatcgc cgccgcgctg gagggggtga gcgaggcgcg
     2041 cctccaggcc atgctcgggc tgccgcggat cccgtggagc cgcaagggcc tggtcgcgca
     2101 gcgcggccag gcctatttgc gccggacgct ggaggacgtg gtccgcgaac gactgccgga
     2161 aggcggcccg gacgactttc tcggccgcct gatccgcggc ctcaacgaac gtttcgagcc
     2221 gaagcaggcc gcggccctcg cggcggacaa tgcgatgacc ttctatctcg ccggccacga
     2281 ccgccaatgc ggtgagctgg gcgcttttcc tcctggccga gcagccggag ctccaggagc
     2341 ggctggccgg ggaggcggca tccgcgctgg cggaagggga gggggccgat cttccggccc
     2401 ggctgccgaa gctgcgcctg gctctggagg agacgctccg cctctacccg tcggcgccgc
     2461 gcttcgaccg ccaggcggtg gagtcggacc gcctctgcgg cgaggaggtc cgacccggcg
     2521 acatcgtctc gatctggccg tggctgctgc accggcacaa ggcgctgtgg gacgatccgg
     2581 acgtgttcga tgccgagcgc ttcgctgagg gaagccggca ccgcttccaa tatatcccgt
     2641 tcggcggcgg cccgcggctg tgcgtcggcg cccgcttcgc caccgccgag gcgctgacca
     2701 tcctcgccca ctggctgagc cattggcgtt tccggccggt cccgggccat gtggtgaggg
     2761 tgcacggcat ggtgaccctg cggccggccg gaggcttgcc gctgatcctg tcccggcgcg
     2821 actgatccac ccgtcctgcg gccgattgct cgtccggcgg cgcacagcag ccggttgacc
     2881 tcctcggcac aagctgtcta agtgctattg cgaataattc tcaagagcga taggacggcc
     2941 gcgcgacgca aagctgcgcg cttgaaaggg gttcgaatgt atcggcgtac cgtctccatg
     3001 gccgccctga cggcggcgct cgcgatgccg gcttccgcgg ccgagctggc cgccaccgct
     3061 gctgcggccg ccgacgccga gccgacgatc gtcgtcaccg gccagcgccc ggaatatgga
     3121 gctcaggaaa cctgcaccgc gacccggacc tgcaccgagg tcaaggacgt gccgcagtcg
     3181 ctgtccgtcg tcaccgagag ccagatcgag gaccaggcga tgcgctcgat cgccgacgtg
     3241 ctgatgtacg tcccgggcgc gacccccggc accggcgagg gcaatcgcga ccagatcacc
     3301 ctgcgcggca acaacacgac cgccgacttc ttcgtcaacg gcgttcgcga cgacgtccaa
     3361 tatttccgcg acctctacaa cgccgagcgc atcgaggtgc ttcgcggccc caacgcgatg
     3421 atcttcggcc gaggcggcgg cggcggcgtc gtcaaccgcg tgaccaagcg ctcgagcttc
     3481 ggcacctatc gcgagctcgc cgtccagggc gacagcgaag gcggcctccg cctgaccggc
     3541 gacgtcgacc agccgctggg cgaatcgatc ggcctcaggc tgaacggcgt ctacgaaaat
     3601 ggggagagct tccggcgcca tgtggagctg gagcgcttcg gcatcaatcc ggtgctcggc
     3661 gccaggcttg gcgacacccg gattgacctc tcctacgaat atttccatga ccgccggacc
     3721 gccgaccgcg gcctgccggc ggccggatcg cggccgctgc gcggcgccga ccgcaccttc
     3781 ttcggcgacc cggacctcag tttcgccgat gtcgacgcgc atgtcgccag cctggccgtc
     3841 gagcaccggt tctccgatgc gctcaccttg cgcaaccgca ccagctacgg ggatttcgac
     3901 aaattctacc agaacatctt tccgtcggct ctgaacacgg ccaccggcct ggtgagcgtg
     3961 tccgcctata atgacagcgt cgatcggcag aacctgttca gccagaccga cctgatcgtc
     4021 gagggcgatc tcggcggctt ctcccacacc ctcctgctcg gcttcgagat cggtcggcag
     4081 gagaccctca gccgccgccg caacggcttc ttcacctcgg cggcgaatcc gctcggagtc
     4141 ccctcgatca gcgtgccggt gacgtcgccg acgatcgacg cgagcctcgt cttcctgccg
     4201 gtcaacacca accccgcgcg caccccggcc aatttcaacg agagcgaagc gaaggtgctc
     4261 gccttctacg cgcaggagca gctgcgcctg tccgacgcga tcgagatcgt cgccggcctg
     4321 cgctacgacc gtttcgagct cgacgtcctc aaccgcaaca ccggccagac cttcagccgc
     4381 accgacgagc tgctctcgcc gcgcctcggg ctggtcgtga agccgatgcc gagcctgtcc
     4441 ctctatgcca gctacggccg ctcctatctg ccgagcggag gggaccagtt cacctcgctc
     4501 gacctcaccg gcgcggcgct caagcccgag cggttcgaca attacgagat cggcgccaaa
     4561 tgggagccgg tccaaggcct gctcgccacg gtcgcgctct accagctcga ccgcaccaac
     4621 acccgcgccg cgggaccgac tccggggacg gtcgtgctga ccggagccca gcgcagccgc
     4681 ggcctcgagc tcgggctcga gcgcaacgtc acggaccgct ggcaggtctc ggccggctat
     4741 gcgcttcagg aggcccggat ccggcgaacg accgcggcgg cgccggccgg ccgcgaggtg
     4801 ccgctcgtgc cgaagcacca attctcggtc tggaaccgct acaatgtcga tcgcgggctc
     4861 ggcttcggcc tcggcgtcgt cgccgcctcc agatcctatg cctcgatcag taatgcggtg
     4921 actctgccgg gctacgcgcg ggtcgacgcc gccgtcttct acgagctgac cgacgggatc
     4981 gaggcgcagg tgaacgtcga gaacctgctc ggcgccgact atttcccgac cgcgcacaac
     5041 gacaacaaca tcgcccccgg cgctccgacc acggcgcgtg cgaccctgcg cttcaacttc
     5101 tgacggcgcg ggagccgcgc acgttgcgcc ggcggttccg aactcctaaa ctgctcctgc
     5161 ggcgcgcttt tcccgggccg cgggagccag acgatgatca agagcctgtt gacgatggtt
     5221 gccctgttgt cagccgcgcc ggcctcggca caggcgggca cgcaagccgg cggctccatt
     5281 cgctcaaacg atcagattcg gggcgcgttg ttcggctcca ccatgtcggc acaggccgaa
     5341 atgaggggcg cggcccacag gcgggaatcg cagcgccgca ccgtttccga cggaaccgcc
//
